Aff4 cDNA ORF clone, Mus musculus(house mouse)

The following Aff4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aff4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu15411 XM_011249331.3
Latest version!
Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu47156 XM_030246443.1
Latest version!
Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu15411 NM_033565.2
Latest version!
Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu47156 NM_001363331.1
Latest version!
Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu47156 XM_006534560.3
Latest version!
Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OMu15411 XM_011249331.2 Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu15411
    Clone ID Related Accession (Same CDS sequence) XM_011249331.3 , NM_033565.2 , XM_011249331.2
    Accession Version XM_011249331.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product AF4/FMR2 family member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249331.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA
    GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT
    GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA
    GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT
    CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG
    AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA
    AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG
    GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG
    GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC
    GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA
    TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT
    GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT
    GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG
    GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GATGACGCAT
    TCATGGCCTC CCCCTCTAAC GGCTATTCAT ACACCATGCA AAACAGAACC TTCCAAATTC
    CCTTTTCCAA CTAAGGAGTC GCAACAGTCT AACTTTGGCC CTGGAGAACA AAAAAGATAC
    AGTACTGCTA AAACATCAAA TGGACACCAG TCAAAATCTA TGTTAAAAGA TGACTTAAAA
    CTAAGCAGCA GTGAAGACAG TGATGGGGAA CAGGATTGTG ATAAGACAAT GCCAAGGAGT
    ACACCAGGAA GTAACTCTGA ACCTTCACAC CATAACAGTG AGGGAGCAGA CAACTCCAGG
    GATGACTCGA GCAGCCACAG TGGATCTGAG AGCAGCTCTG GGTCTGACTC GGAGAGTGAG
    AGCAGCTCCA GTGACAGTGA GGCTAATGAG CCGTCGCAGA GCGCATCTCC TGAGCCTGAA
    CCACCACCAA CAAACAAGTG GCAACTTGAT AATTGGTTGA ATAAAGTGAA CCCACATAAA
    GTATCCCCAG CTTCCTCTGT AGACAGCAAC ATCCCATCTT CTCAAGCCTA CAAGAAAGAA
    GGCCGGGAGC AGGGCACTGC CAGTAACTAC ACAGATCCAG GGGGGACAAA AGAAACGAGC
    TCTGCCACCC CAGGACGAGA CTCTAAAACC ATCCAAAAGG GATCAGAAAG TGGCCGTGGG
    AGGCAGAAGT CTCCTGCACA GAGCGACAGT ACCACTCAGA GGAGAACTGT CGGCAAAAAA
    CAACCCAAAA AACCTGAGAA GTCAGCTGCT GAAGAGCCTC GTGGAGGCCT GAAGATAGAA
    AGTGAGACCC CTGTGGACAT GGCTGCCAGC ATGCCCTCCA GCAGGCACAA AGCAGCCACC
    AAGGGCTCGA GGAAACCCAA CATCAAAAAG GAATCAAAAT CTTCTCCTCG ACCTACGGCA
    GAGAAGAAGA AGTACAAGTC AGCCAGTAAA CCGTCCCAGA AGTCTCGGGA GATCATAGAG
    ACTGACACCT CGTCCTCAGA CTCGGACGGC AGCGAGAGCC TGCCTCCCTC CTCACAGACT
    CCTAAGTACC CTGAAAGCAA CAGGACTCCT GTCAAACCCT CCTCAGTGGA GGAAGAAGAT
    AGCTTTTTTC GGCAACGAAT GTTCTCTCCT ATGGAAGAGA AGGAGCTCCT TTCCCCACTC
    AGTGAGCCTG ATGACAGGTA TCCACTCATT GTGAAGATTG ACCTCAATCT TCTGACTCGA
    ATACCCGGGA AGCCTTACAA AGAAACAGAG CCACCCAAGG GGGAAAAGAA AAATGTTCCA
    GAGAAACACT CGAGAGAGGT GCAGAAGCAA GCCTCAGAAA AGGCTTCCAA CAAAGGCAAG
    AGGAAACACA AGAATGATGA TGATACCCGA GCCAGTGAGA GCAAAAAACC CAAAACAGAG
    GACAAGAACT CATCAGGCCA CAAGCCATCC AGCAGCAGAG AGTCATCTAA GCAGAGTAGT
    ACAAAAGAAA AGGACTTGCT ACCTTCACCT GCTGGGCCCA TTCTTTCAAA AGATTCAAAA
    ACAGAGCATG GTTCTCGGAA GAGGACTGTC AGTCAGTCGT CGTCCTTAAA GTCCAGTGGG
    ACCAGCAGCA AAGAGAACAG TGGCAGCAGC AGCAAGAGCA GCTCCTCCTC CACAGCGAAG
    CAGAAGAAGA CTGAAGGCAA GGGTCCCAGC AGCTCCAAGG AGGCCAAGGA GAAGGCTCCA
    AATAGCTCCT CTAATTGTCC TCCGTCTACA CCAACTTCTG AGTCTTCGAA GCCTCGGAGA
    ACAAAGCTTG CCTTTGATGA CAGAAATTAT TCAGCAGACC ATTATTTACA AGAAGCAAAA
    AAACTCAAAC ACAATGCAGA TGCTTTGTCT GACAGGTTTG AGAAAGCCGT GTACTACCTT
    GATGCTGTAG TGTCTTTCAT TGAGTGTGGG AATGCATTAG AGAAGAACGC TCAGGAATCC
    AAGTCCCCAT TCCCTATGTA CTCAGACACG GTGGAGCTCA TTAAGTACAC TATGAAACTA
    AAGAATTATT TGGCACCAGA TGCTACAGCT GCAGACAAGA GACTCACAGT ACTTTGCCTG
    AGATGCCAGT CATTATTGTA CCTGAGGCTG TTCAAACTGA AGAAGGAGAA CGCTCTCAAG
    TACTCCAAGA CGCTAACTGA GCACCTGAAG AATTCTTACA GTAATTCTCA AGCACCTTCA
    CCCGGCTTGG GGAGCAAAGC TGTTGGGATG CCCTCCCCTG TTTCTCCAAA ACTGTCACCA
    GGCAATTCTG GAAGTTACTC TTCTGGGGGA AGTAGTGCTT CAGCAAGTGG TTCTTCAGTG
    ACCATTCCAC AGAAAATCCA CCAGATGGCA GCAAGCTATG TCCAGGTTAC ATCTAACTTC
    CTCTATGCCA CAGAAATTTG GGACCAAGCT GAACAGCTTT CCAAAGAACA AAAAGAATTC
    TTTGCTGAAC TGGACAAAGT GATGGGCCCT CTCATCTTTA ATGCAAGCAT CATGACAGAT
    CTGGCTCGTT ACACCCGGCA GGGACTGCAT TGGCTTCGGC AGGATGCCAA GCTGATATCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011247633.1
    CDS526..4008
    Translation

    Target ORF information:

    RefSeq Version XM_011249331.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011249331.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA
    GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT
    GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA
    GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT
    CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG
    AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA
    AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG
    GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG
    GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC
    GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA
    TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT
    GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT
    GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG
    GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GATGACGCAT
    TCATGGCCTC CCCCTCTAAC GGCTATTCAT ACACCATGCA AAACAGAACC TTCCAAATTC
    CCTTTTCCAA CTAAGGAGTC GCAACAGTCT AACTTTGGCC CTGGAGAACA AAAAAGATAC
    AGTACTGCTA AAACATCAAA TGGACACCAG TCAAAATCTA TGTTAAAAGA TGACTTAAAA
    CTAAGCAGCA GTGAAGACAG TGATGGGGAA CAGGATTGTG ATAAGACAAT GCCAAGGAGT
    ACACCAGGAA GTAACTCTGA ACCTTCACAC CATAACAGTG AGGGAGCAGA CAACTCCAGG
    GATGACTCGA GCAGCCACAG TGGATCTGAG AGCAGCTCTG GGTCTGACTC GGAGAGTGAG
    AGCAGCTCCA GTGACAGTGA GGCTAATGAG CCGTCGCAGA GCGCATCTCC TGAGCCTGAA
    CCACCACCAA CAAACAAGTG GCAACTTGAT AATTGGTTGA ATAAAGTGAA CCCACATAAA
    GTATCCCCAG CTTCCTCTGT AGACAGCAAC ATCCCATCTT CTCAAGCCTA CAAGAAAGAA
    GGCCGGGAGC AGGGCACTGC CAGTAACTAC ACAGATCCAG GGGGGACAAA AGAAACGAGC
    TCTGCCACCC CAGGACGAGA CTCTAAAACC ATCCAAAAGG GATCAGAAAG TGGCCGTGGG
    AGGCAGAAGT CTCCTGCACA GAGCGACAGT ACCACTCAGA GGAGAACTGT CGGCAAAAAA
    CAACCCAAAA AACCTGAGAA GTCAGCTGCT GAAGAGCCTC GTGGAGGCCT GAAGATAGAA
    AGTGAGACCC CTGTGGACAT GGCTGCCAGC ATGCCCTCCA GCAGGCACAA AGCAGCCACC
    AAGGGCTCGA GGAAACCCAA CATCAAAAAG GAATCAAAAT CTTCTCCTCG ACCTACGGCA
    GAGAAGAAGA AGTACAAGTC AGCCAGTAAA CCGTCCCAGA AGTCTCGGGA GATCATAGAG
    ACTGACACCT CGTCCTCAGA CTCGGACGGC AGCGAGAGCC TGCCTCCCTC CTCACAGACT
    CCTAAGTACC CTGAAAGCAA CAGGACTCCT GTCAAACCCT CCTCAGTGGA GGAAGAAGAT
    AGCTTTTTTC GGCAACGAAT GTTCTCTCCT ATGGAAGAGA AGGAGCTCCT TTCCCCACTC
    AGTGAGCCTG ATGACAGGTA TCCACTCATT GTGAAGATTG ACCTCAATCT TCTGACTCGA
    ATACCCGGGA AGCCTTACAA AGAAACAGAG CCACCCAAGG GGGAAAAGAA AAATGTTCCA
    GAGAAACACT CGAGAGAGGT GCAGAAGCAA GCCTCAGAAA AGGCTTCCAA CAAAGGCAAG
    AGGAAACACA AGAATGATGA TGATACCCGA GCCAGTGAGA GCAAAAAACC CAAAACAGAG
    GACAAGAACT CATCAGGCCA CAAGCCATCC AGCAGCAGAG AGTCATCTAA GCAGAGTAGT
    ACAAAAGAAA AGGACTTGCT ACCTTCACCT GCTGGGCCCA TTCTTTCAAA AGATTCAAAA
    ACAGAGCATG GTTCTCGGAA GAGGACTGTC AGTCAGTCGT CGTCCTTAAA GTCCAGTGGG
    ACCAGCAGCA AAGAGAACAG TGGCAGCAGC AGCAAGAGCA GCTCCTCCTC CACAGCGAAG
    CAGAAGAAGA CTGAAGGCAA GGGTCCCAGC AGCTCCAAGG AGGCCAAGGA GAAGGCTCCA
    AATAGCTCCT CTAATTGTCC TCCGTCTACA CCAACTTCTG AGTCTTCGAA GCCTCGGAGA
    ACAAAGCTTG CCTTTGATGA CAGAAATTAT TCAGCAGACC ATTATTTACA AGAAGCAAAA
    AAACTCAAAC ACAATGCAGA TGCTTTGTCT GACAGGTTTG AGAAAGCCGT GTACTACCTT
    GATGCTGTAG TGTCTTTCAT TGAGTGTGGG AATGCATTAG AGAAGAACGC TCAGGAATCC
    AAGTCCCCAT TCCCTATGTA CTCAGACACG GTGGAGCTCA TTAAGTACAC TATGAAACTA
    AAGAATTATT TGGCACCAGA TGCTACAGCT GCAGACAAGA GACTCACAGT ACTTTGCCTG
    AGATGCCAGT CATTATTGTA CCTGAGGCTG TTCAAACTGA AGAAGGAGAA CGCTCTCAAG
    TACTCCAAGA CGCTAACTGA GCACCTGAAG AATTCTTACA GTAATTCTCA AGCACCTTCA
    CCCGGCTTGG GGAGCAAAGC TGTTGGGATG CCCTCCCCTG TTTCTCCAAA ACTGTCACCA
    GGCAATTCTG GAAGTTACTC TTCTGGGGGA AGTAGTGCTT CAGCAAGTGG TTCTTCAGTG
    ACCATTCCAC AGAAAATCCA CCAGATGGCA GCAAGCTATG TCCAGGTTAC ATCTAACTTC
    CTCTATGCCA CAGAAATTTG GGACCAAGCT GAACAGCTTT CCAAAGAACA AAAAGAATTC
    TTTGCTGAAC TGGACAAAGT GATGGGCCCT CTCATCTTTA ATGCAAGCAT CATGACAGAT
    CTGGCTCGTT ACACCCGGCA GGGACTGCAT TGGCTTCGGC AGGATGCCAA GCTGATATCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu47156
    Clone ID Related Accession (Same CDS sequence) NM_001363331.1 , XM_006534560.3 , XM_030246443.1
    Accession Version XM_030246443.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product AF4/FMR2 family member 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA
    GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT
    GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA
    GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT
    CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG
    AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA
    AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG
    GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG
    GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC
    GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA
    TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT
    GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT
    GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG
    GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GTCGCAACAG
    TCTAACTTTG GCCCTGGAGA ACAAAAAAGA TACAGTACTG CTAAAACATC AAATGGACAC
    CAGTCAAAAT CTATGTTAAA AGATGACTTA AAACTAAGCA GCAGTGAAGA CAGTGATGGG
    GAACAGGATT GTGATAAGAC AATGCCAAGG AGTACACCAG GAAGTAACTC TGAACCTTCA
    CACCATAACA GTGAGGGAGC AGACAACTCC AGGGATGACT CGAGCAGCCA CAGTGGATCT
    GAGAGCAGCT CTGGGTCTGA CTCGGAGAGT GAGAGCAGCT CCAGTGACAG TGAGGCTAAT
    GAGCCGTCGC AGAGCGCATC TCCTGAGCCT GAACCACCAC CAACAAACAA GTGGCAACTT
    GATAATTGGT TGAATAAAGT GAACCCACAT AAAGTATCCC CAGCTTCCTC TGTAGACAGC
    AACATCCCAT CTTCTCAAGC CTACAAGAAA GAAGGCCGGG AGCAGGGCAC TGCCAGTAAC
    TACACAGATC CAGGGGGGAC AAAAGAAACG AGCTCTGCCA CCCCAGGACG AGACTCTAAA
    ACCATCCAAA AGGGATCAGA AAGTGGCCGT GGGAGGCAGA AGTCTCCTGC ACAGAGCGAC
    AGTACCACTC AGAGGAGAAC TGTCGGCAAA AAACAACCCA AAAAACCTGA GAAGTCAGCT
    GCTGAAGAGC CTCGTGGAGG CCTGAAGATA GAAAGTGAGA CCCCTGTGGA CATGGCTGCC
    AGCATGCCCT CCAGCAGGCA CAAAGCAGCC ACCAAGGGCT CGAGGAAACC CAACATCAAA
    AAGGAATCAA AATCTTCTCC TCGACCTACG GCAGAGAAGA AGAAGTACAA GTCAGCCAGT
    AAACCGTCCC AGAAGTCTCG GGAGATCATA GAGACTGACA CCTCGTCCTC AGACTCGGAC
    GGCAGCGAGA GCCTGCCTCC CTCCTCACAG ACTCCTAAGT ACCCTGAAAG CAACAGGACT
    CCTGTCAAAC CCTCCTCAGT GGAGGAAGAA GATAGCTTTT TTCGGCAACG AATGTTCTCT
    CCTATGGAAG AGAAGGAGCT CCTTTCCCCA CTCAGTGAGC CTGATGACAG GTATCCACTC
    ATTGTGAAGA TTGACCTCAA TCTTCTGACT CGAATACCCG GGAAGCCTTA CAAAGAAACA
    GAGCCACCCA AGGGGGAAAA GAAAAATGTT CCAGAGAAAC ACTCGAGAGA GGTGCAGAAG
    CAAGCCTCAG AAAAGGCTTC CAACAAAGGC AAGAGGAAAC ACAAGAATGA TGATGATACC
    CGAGCCAGTG AGAGCAAAAA ACCCAAAACA GAGGACAAGA ACTCATCAGG CCACAAGCCA
    TCCAGCAGCA GAGAGTCATC TAAGCAGAGT AGTACAAAAG AAAAGGACTT GCTACCTTCA
    CCTGCTGGGC CCATTCTTTC AAAAGATTCA AAAACAGAGC ATGGTTCTCG GAAGAGGACT
    GTCAGTCAGT CGTCGTCCTT AAAGTCCAGT GGGACCAGCA GCAAAGAGAA CAGTGGCAGC
    AGCAGCAAGA GCAGCTCCTC CTCCACAGCG AAGCAGAAGA AGACTGAAGG CAAGGGTCCC
    AGCAGCTCCA AGGAGGCCAA GGAGAAGGCT CCAAATAGCT CCTCTAATTG TCCTCCGTCT
    ACACCAACTT CTGAGTCTTC GAAGCCTCGG AGAACAAAGC TTGCCTTTGA TGACAGAAAT
    TATTCAGCAG ACCATTATTT ACAAGAAGCA AAAAAACTCA AACACAATGC AGATGCTTTG
    TCTGACAGGT TTGAGAAAGC CGTGTACTAC CTTGATGCTG TAGTGTCTTT CATTGAGTGT
    GGGAATGCAT TAGAGAAGAA CGCTCAGGAA TCCAAGTCCC CATTCCCTAT GTACTCAGAC
    ACGGTGGAGC TCATTAAGTA CACTATGAAA CTAAAGAATT ATTTGGCACC AGATGCTACA
    GCTGCAGACA AGAGACTCAC AGTACTTTGC CTGAGATGCC AGTCATTATT GTACCTGAGG
    CTGTTCAAAC TGAAGAAGGA GAACGCTCTC AAGTACTCCA AGACGCTAAC TGAGCACCTG
    AAGAATTCTT ACAGTAATTC TCAAGCACCT TCACCCGGCT TGGGGAGCAA AGCTGTTGGG
    ATGCCCTCCC CTGTTTCTCC AAAACTGTCA CCAGGCAATT CTGGAAGTTA CTCTTCTGGG
    GGAAGTAGTG CTTCAGCAAG TGGTTCTTCA GTGACCATTC CACAGAAAAT CCACCAGATG
    GCAGCAAGCT ATGTCCAGGT TACATCTAAC TTCCTCTATG CCACAGAAAT TTGGGACCAA
    GCTGAACAGC TTTCCAAAGA ACAAAAAGAA TTCTTTGCTG AACTGGACAA AGTGATGGGC
    CCTCTCATCT TTAATGCAAG CATCATGACA GATCTGGCTC GTTACACCCG GCAGGGACTG
    CATTGGCTTC GGCAGGATGC CAAGCTGATA TCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030102303.1
    CDS587..3982
    Translation

    Target ORF information:

    RefSeq Version XM_030246443.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030246443.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA
    GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT
    GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA
    GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT
    CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG
    AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA
    AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG
    GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG
    GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC
    GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA
    TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT
    GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT
    GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG
    GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GTCGCAACAG
    TCTAACTTTG GCCCTGGAGA ACAAAAAAGA TACAGTACTG CTAAAACATC AAATGGACAC
    CAGTCAAAAT CTATGTTAAA AGATGACTTA AAACTAAGCA GCAGTGAAGA CAGTGATGGG
    GAACAGGATT GTGATAAGAC AATGCCAAGG AGTACACCAG GAAGTAACTC TGAACCTTCA
    CACCATAACA GTGAGGGAGC AGACAACTCC AGGGATGACT CGAGCAGCCA CAGTGGATCT
    GAGAGCAGCT CTGGGTCTGA CTCGGAGAGT GAGAGCAGCT CCAGTGACAG TGAGGCTAAT
    GAGCCGTCGC AGAGCGCATC TCCTGAGCCT GAACCACCAC CAACAAACAA GTGGCAACTT
    GATAATTGGT TGAATAAAGT GAACCCACAT AAAGTATCCC CAGCTTCCTC TGTAGACAGC
    AACATCCCAT CTTCTCAAGC CTACAAGAAA GAAGGCCGGG AGCAGGGCAC TGCCAGTAAC
    TACACAGATC CAGGGGGGAC AAAAGAAACG AGCTCTGCCA CCCCAGGACG AGACTCTAAA
    ACCATCCAAA AGGGATCAGA AAGTGGCCGT GGGAGGCAGA AGTCTCCTGC ACAGAGCGAC
    AGTACCACTC AGAGGAGAAC TGTCGGCAAA AAACAACCCA AAAAACCTGA GAAGTCAGCT
    GCTGAAGAGC CTCGTGGAGG CCTGAAGATA GAAAGTGAGA CCCCTGTGGA CATGGCTGCC
    AGCATGCCCT CCAGCAGGCA CAAAGCAGCC ACCAAGGGCT CGAGGAAACC CAACATCAAA
    AAGGAATCAA AATCTTCTCC TCGACCTACG GCAGAGAAGA AGAAGTACAA GTCAGCCAGT
    AAACCGTCCC AGAAGTCTCG GGAGATCATA GAGACTGACA CCTCGTCCTC AGACTCGGAC
    GGCAGCGAGA GCCTGCCTCC CTCCTCACAG ACTCCTAAGT ACCCTGAAAG CAACAGGACT
    CCTGTCAAAC CCTCCTCAGT GGAGGAAGAA GATAGCTTTT TTCGGCAACG AATGTTCTCT
    CCTATGGAAG AGAAGGAGCT CCTTTCCCCA CTCAGTGAGC CTGATGACAG GTATCCACTC
    ATTGTGAAGA TTGACCTCAA TCTTCTGACT CGAATACCCG GGAAGCCTTA CAAAGAAACA
    GAGCCACCCA AGGGGGAAAA GAAAAATGTT CCAGAGAAAC ACTCGAGAGA GGTGCAGAAG
    CAAGCCTCAG AAAAGGCTTC CAACAAAGGC AAGAGGAAAC ACAAGAATGA TGATGATACC
    CGAGCCAGTG AGAGCAAAAA ACCCAAAACA GAGGACAAGA ACTCATCAGG CCACAAGCCA
    TCCAGCAGCA GAGAGTCATC TAAGCAGAGT AGTACAAAAG AAAAGGACTT GCTACCTTCA
    CCTGCTGGGC CCATTCTTTC AAAAGATTCA AAAACAGAGC ATGGTTCTCG GAAGAGGACT
    GTCAGTCAGT CGTCGTCCTT AAAGTCCAGT GGGACCAGCA GCAAAGAGAA CAGTGGCAGC
    AGCAGCAAGA GCAGCTCCTC CTCCACAGCG AAGCAGAAGA AGACTGAAGG CAAGGGTCCC
    AGCAGCTCCA AGGAGGCCAA GGAGAAGGCT CCAAATAGCT CCTCTAATTG TCCTCCGTCT
    ACACCAACTT CTGAGTCTTC GAAGCCTCGG AGAACAAAGC TTGCCTTTGA TGACAGAAAT
    TATTCAGCAG ACCATTATTT ACAAGAAGCA AAAAAACTCA AACACAATGC AGATGCTTTG
    TCTGACAGGT TTGAGAAAGC CGTGTACTAC CTTGATGCTG TAGTGTCTTT CATTGAGTGT
    GGGAATGCAT TAGAGAAGAA CGCTCAGGAA TCCAAGTCCC CATTCCCTAT GTACTCAGAC
    ACGGTGGAGC TCATTAAGTA CACTATGAAA CTAAAGAATT ATTTGGCACC AGATGCTACA
    GCTGCAGACA AGAGACTCAC AGTACTTTGC CTGAGATGCC AGTCATTATT GTACCTGAGG
    CTGTTCAAAC TGAAGAAGGA GAACGCTCTC AAGTACTCCA AGACGCTAAC TGAGCACCTG
    AAGAATTCTT ACAGTAATTC TCAAGCACCT TCACCCGGCT TGGGGAGCAA AGCTGTTGGG
    ATGCCCTCCC CTGTTTCTCC AAAACTGTCA CCAGGCAATT CTGGAAGTTA CTCTTCTGGG
    GGAAGTAGTG CTTCAGCAAG TGGTTCTTCA GTGACCATTC CACAGAAAAT CCACCAGATG
    GCAGCAAGCT ATGTCCAGGT TACATCTAAC TTCCTCTATG CCACAGAAAT TTGGGACCAA
    GCTGAACAGC TTTCCAAAGA ACAAAAAGAA TTCTTTGCTG AACTGGACAA AGTGATGGGC
    CCTCTCATCT TTAATGCAAG CATCATGACA GATCTGGCTC GTTACACCCG GCAGGGACTG
    CATTGGCTTC GGCAGGATGC CAAGCTGATA TCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu15411
    Clone ID Related Accession (Same CDS sequence) XM_011249331.3 , NM_033565.2 , XM_011249331.2
    Accession Version NM_033565.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Mus musculus(house mouse)
    Product AF4/FMR2 family member 4 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ064959.1, BC138999.1 and AI536326.1. On Jul 18, 2009 this sequence version replaced NM_033565.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF190449.1, BC138999.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA
    GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT
    GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA
    GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT
    CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG
    AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA
    AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG
    GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG
    GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC
    GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA
    TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT
    GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT
    GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG
    GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GATGACGCAT
    TCATGGCCTC CCCCTCTAAC GGCTATTCAT ACACCATGCA AAACAGAACC TTCCAAATTC
    CCTTTTCCAA CTAAGGAGTC GCAACAGTCT AACTTTGGCC CTGGAGAACA AAAAAGATAC
    AGTACTGCTA AAACATCAAA TGGACACCAG TCAAAATCTA TGTTAAAAGA TGACTTAAAA
    CTAAGCAGCA GTGAAGACAG TGATGGGGAA CAGGATTGTG ATAAGACAAT GCCAAGGAGT
    ACACCAGGAA GTAACTCTGA ACCTTCACAC CATAACAGTG AGGGAGCAGA CAACTCCAGG
    GATGACTCGA GCAGCCACAG TGGATCTGAG AGCAGCTCTG GGTCTGACTC GGAGAGTGAG
    AGCAGCTCCA GTGACAGTGA GGCTAATGAG CCGTCGCAGA GCGCATCTCC TGAGCCTGAA
    CCACCACCAA CAAACAAGTG GCAACTTGAT AATTGGTTGA ATAAAGTGAA CCCACATAAA
    GTATCCCCAG CTTCCTCTGT AGACAGCAAC ATCCCATCTT CTCAAGCCTA CAAGAAAGAA
    GGCCGGGAGC AGGGCACTGC CAGTAACTAC ACAGATCCAG GGGGGACAAA AGAAACGAGC
    TCTGCCACCC CAGGACGAGA CTCTAAAACC ATCCAAAAGG GATCAGAAAG TGGCCGTGGG
    AGGCAGAAGT CTCCTGCACA GAGCGACAGT ACCACTCAGA GGAGAACTGT CGGCAAAAAA
    CAACCCAAAA AACCTGAGAA GTCAGCTGCT GAAGAGCCTC GTGGAGGCCT GAAGATAGAA
    AGTGAGACCC CTGTGGACAT GGCTGCCAGC ATGCCCTCCA GCAGGCACAA AGCAGCCACC
    AAGGGCTCGA GGAAACCCAA CATCAAAAAG GAATCAAAAT CTTCTCCTCG ACCTACGGCA
    GAGAAGAAGA AGTACAAGTC AGCCAGTAAA CCGTCCCAGA AGTCTCGGGA GATCATAGAG
    ACTGACACCT CGTCCTCAGA CTCGGACGGC AGCGAGAGCC TGCCTCCCTC CTCACAGACT
    CCTAAGTACC CTGAAAGCAA CAGGACTCCT GTCAAACCCT CCTCAGTGGA GGAAGAAGAT
    AGCTTTTTTC GGCAACGAAT GTTCTCTCCT ATGGAAGAGA AGGAGCTCCT TTCCCCACTC
    AGTGAGCCTG ATGACAGGTA TCCACTCATT GTGAAGATTG ACCTCAATCT TCTGACTCGA
    ATACCCGGGA AGCCTTACAA AGAAACAGAG CCACCCAAGG GGGAAAAGAA AAATGTTCCA
    GAGAAACACT CGAGAGAGGT GCAGAAGCAA GCCTCAGAAA AGGCTTCCAA CAAAGGCAAG
    AGGAAACACA AGAATGATGA TGATACCCGA GCCAGTGAGA GCAAAAAACC CAAAACAGAG
    GACAAGAACT CATCAGGCCA CAAGCCATCC AGCAGCAGAG AGTCATCTAA GCAGAGTAGT
    ACAAAAGAAA AGGACTTGCT ACCTTCACCT GCTGGGCCCA TTCTTTCAAA AGATTCAAAA
    ACAGAGCATG GTTCTCGGAA GAGGACTGTC AGTCAGTCGT CGTCCTTAAA GTCCAGTGGG
    ACCAGCAGCA AAGAGAACAG TGGCAGCAGC AGCAAGAGCA GCTCCTCCTC CACAGCGAAG
    CAGAAGAAGA CTGAAGGCAA GGGTCCCAGC AGCTCCAAGG AGGCCAAGGA GAAGGCTCCA
    AATAGCTCCT CTAATTGTCC TCCGTCTACA CCAACTTCTG AGTCTTCGAA GCCTCGGAGA
    ACAAAGCTTG CCTTTGATGA CAGAAATTAT TCAGCAGACC ATTATTTACA AGAAGCAAAA
    AAACTCAAAC ACAATGCAGA TGCTTTGTCT GACAGGTTTG AGAAAGCCGT GTACTACCTT
    GATGCTGTAG TGTCTTTCAT TGAGTGTGGG AATGCATTAG AGAAGAACGC TCAGGAATCC
    AAGTCCCCAT TCCCTATGTA CTCAGACACG GTGGAGCTCA TTAAGTACAC TATGAAACTA
    AAGAATTATT TGGCACCAGA TGCTACAGCT GCAGACAAGA GACTCACAGT ACTTTGCCTG
    AGATGCCAGT CATTATTGTA CCTGAGGCTG TTCAAACTGA AGAAGGAGAA CGCTCTCAAG
    TACTCCAAGA CGCTAACTGA GCACCTGAAG AATTCTTACA GTAATTCTCA AGCACCTTCA
    CCCGGCTTGG GGAGCAAAGC TGTTGGGATG CCCTCCCCTG TTTCTCCAAA ACTGTCACCA
    GGCAATTCTG GAAGTTACTC TTCTGGGGGA AGTAGTGCTT CAGCAAGTGG TTCTTCAGTG
    ACCATTCCAC AGAAAATCCA CCAGATGGCA GCAAGCTATG TCCAGGTTAC ATCTAACTTC
    CTCTATGCCA CAGAAATTTG GGACCAAGCT GAACAGCTTT CCAAAGAACA AAAAGAATTC
    TTTGCTGAAC TGGACAAAGT GATGGGCCCT CTCATCTTTA ATGCAAGCAT CATGACAGAT
    CTGGCTCGTT ACACCCGGCA GGGACTGCAT TGGCTTCGGC AGGATGCCAA GCTGATATCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_291043.1
    CDS368..3850
    Translation

    Target ORF information:

    RefSeq Version NM_033565.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033565.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA
    GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT
    GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA
    GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT
    CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG
    AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA
    AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG
    GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG
    GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC
    GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA
    TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT
    GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT
    GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG
    GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GATGACGCAT
    TCATGGCCTC CCCCTCTAAC GGCTATTCAT ACACCATGCA AAACAGAACC TTCCAAATTC
    CCTTTTCCAA CTAAGGAGTC GCAACAGTCT AACTTTGGCC CTGGAGAACA AAAAAGATAC
    AGTACTGCTA AAACATCAAA TGGACACCAG TCAAAATCTA TGTTAAAAGA TGACTTAAAA
    CTAAGCAGCA GTGAAGACAG TGATGGGGAA CAGGATTGTG ATAAGACAAT GCCAAGGAGT
    ACACCAGGAA GTAACTCTGA ACCTTCACAC CATAACAGTG AGGGAGCAGA CAACTCCAGG
    GATGACTCGA GCAGCCACAG TGGATCTGAG AGCAGCTCTG GGTCTGACTC GGAGAGTGAG
    AGCAGCTCCA GTGACAGTGA GGCTAATGAG CCGTCGCAGA GCGCATCTCC TGAGCCTGAA
    CCACCACCAA CAAACAAGTG GCAACTTGAT AATTGGTTGA ATAAAGTGAA CCCACATAAA
    GTATCCCCAG CTTCCTCTGT AGACAGCAAC ATCCCATCTT CTCAAGCCTA CAAGAAAGAA
    GGCCGGGAGC AGGGCACTGC CAGTAACTAC ACAGATCCAG GGGGGACAAA AGAAACGAGC
    TCTGCCACCC CAGGACGAGA CTCTAAAACC ATCCAAAAGG GATCAGAAAG TGGCCGTGGG
    AGGCAGAAGT CTCCTGCACA GAGCGACAGT ACCACTCAGA GGAGAACTGT CGGCAAAAAA
    CAACCCAAAA AACCTGAGAA GTCAGCTGCT GAAGAGCCTC GTGGAGGCCT GAAGATAGAA
    AGTGAGACCC CTGTGGACAT GGCTGCCAGC ATGCCCTCCA GCAGGCACAA AGCAGCCACC
    AAGGGCTCGA GGAAACCCAA CATCAAAAAG GAATCAAAAT CTTCTCCTCG ACCTACGGCA
    GAGAAGAAGA AGTACAAGTC AGCCAGTAAA CCGTCCCAGA AGTCTCGGGA GATCATAGAG
    ACTGACACCT CGTCCTCAGA CTCGGACGGC AGCGAGAGCC TGCCTCCCTC CTCACAGACT
    CCTAAGTACC CTGAAAGCAA CAGGACTCCT GTCAAACCCT CCTCAGTGGA GGAAGAAGAT
    AGCTTTTTTC GGCAACGAAT GTTCTCTCCT ATGGAAGAGA AGGAGCTCCT TTCCCCACTC
    AGTGAGCCTG ATGACAGGTA TCCACTCATT GTGAAGATTG ACCTCAATCT TCTGACTCGA
    ATACCCGGGA AGCCTTACAA AGAAACAGAG CCACCCAAGG GGGAAAAGAA AAATGTTCCA
    GAGAAACACT CGAGAGAGGT GCAGAAGCAA GCCTCAGAAA AGGCTTCCAA CAAAGGCAAG
    AGGAAACACA AGAATGATGA TGATACCCGA GCCAGTGAGA GCAAAAAACC CAAAACAGAG
    GACAAGAACT CATCAGGCCA CAAGCCATCC AGCAGCAGAG AGTCATCTAA GCAGAGTAGT
    ACAAAAGAAA AGGACTTGCT ACCTTCACCT GCTGGGCCCA TTCTTTCAAA AGATTCAAAA
    ACAGAGCATG GTTCTCGGAA GAGGACTGTC AGTCAGTCGT CGTCCTTAAA GTCCAGTGGG
    ACCAGCAGCA AAGAGAACAG TGGCAGCAGC AGCAAGAGCA GCTCCTCCTC CACAGCGAAG
    CAGAAGAAGA CTGAAGGCAA GGGTCCCAGC AGCTCCAAGG AGGCCAAGGA GAAGGCTCCA
    AATAGCTCCT CTAATTGTCC TCCGTCTACA CCAACTTCTG AGTCTTCGAA GCCTCGGAGA
    ACAAAGCTTG CCTTTGATGA CAGAAATTAT TCAGCAGACC ATTATTTACA AGAAGCAAAA
    AAACTCAAAC ACAATGCAGA TGCTTTGTCT GACAGGTTTG AGAAAGCCGT GTACTACCTT
    GATGCTGTAG TGTCTTTCAT TGAGTGTGGG AATGCATTAG AGAAGAACGC TCAGGAATCC
    AAGTCCCCAT TCCCTATGTA CTCAGACACG GTGGAGCTCA TTAAGTACAC TATGAAACTA
    AAGAATTATT TGGCACCAGA TGCTACAGCT GCAGACAAGA GACTCACAGT ACTTTGCCTG
    AGATGCCAGT CATTATTGTA CCTGAGGCTG TTCAAACTGA AGAAGGAGAA CGCTCTCAAG
    TACTCCAAGA CGCTAACTGA GCACCTGAAG AATTCTTACA GTAATTCTCA AGCACCTTCA
    CCCGGCTTGG GGAGCAAAGC TGTTGGGATG CCCTCCCCTG TTTCTCCAAA ACTGTCACCA
    GGCAATTCTG GAAGTTACTC TTCTGGGGGA AGTAGTGCTT CAGCAAGTGG TTCTTCAGTG
    ACCATTCCAC AGAAAATCCA CCAGATGGCA GCAAGCTATG TCCAGGTTAC ATCTAACTTC
    CTCTATGCCA CAGAAATTTG GGACCAAGCT GAACAGCTTT CCAAAGAACA AAAAGAATTC
    TTTGCTGAAC TGGACAAAGT GATGGGCCCT CTCATCTTTA ATGCAAGCAT CATGACAGAT
    CTGGCTCGTT ACACCCGGCA GGGACTGCAT TGGCTTCGGC AGGATGCCAA GCTGATATCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu47156
    Clone ID Related Accession (Same CDS sequence) NM_001363331.1 , XM_006534560.3 , XM_030246443.1
    Accession Version NM_001363331.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-16
    Organism Mus musculus(house mouse)
    Product AF4/FMR2 family member 4 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL592489.13. On May 17, 2018 this sequence version replaced XM_006534560.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC145490.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA
    GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT
    GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA
    GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT
    CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG
    AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA
    AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG
    GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG
    GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC
    GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA
    TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT
    GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT
    GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG
    GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GTCGCAACAG
    TCTAACTTTG GCCCTGGAGA ACAAAAAAGA TACAGTACTG CTAAAACATC AAATGGACAC
    CAGTCAAAAT CTATGTTAAA AGATGACTTA AAACTAAGCA GCAGTGAAGA CAGTGATGGG
    GAACAGGATT GTGATAAGAC AATGCCAAGG AGTACACCAG GAAGTAACTC TGAACCTTCA
    CACCATAACA GTGAGGGAGC AGACAACTCC AGGGATGACT CGAGCAGCCA CAGTGGATCT
    GAGAGCAGCT CTGGGTCTGA CTCGGAGAGT GAGAGCAGCT CCAGTGACAG TGAGGCTAAT
    GAGCCGTCGC AGAGCGCATC TCCTGAGCCT GAACCACCAC CAACAAACAA GTGGCAACTT
    GATAATTGGT TGAATAAAGT GAACCCACAT AAAGTATCCC CAGCTTCCTC TGTAGACAGC
    AACATCCCAT CTTCTCAAGC CTACAAGAAA GAAGGCCGGG AGCAGGGCAC TGCCAGTAAC
    TACACAGATC CAGGGGGGAC AAAAGAAACG AGCTCTGCCA CCCCAGGACG AGACTCTAAA
    ACCATCCAAA AGGGATCAGA AAGTGGCCGT GGGAGGCAGA AGTCTCCTGC ACAGAGCGAC
    AGTACCACTC AGAGGAGAAC TGTCGGCAAA AAACAACCCA AAAAACCTGA GAAGTCAGCT
    GCTGAAGAGC CTCGTGGAGG CCTGAAGATA GAAAGTGAGA CCCCTGTGGA CATGGCTGCC
    AGCATGCCCT CCAGCAGGCA CAAAGCAGCC ACCAAGGGCT CGAGGAAACC CAACATCAAA
    AAGGAATCAA AATCTTCTCC TCGACCTACG GCAGAGAAGA AGAAGTACAA GTCAGCCAGT
    AAACCGTCCC AGAAGTCTCG GGAGATCATA GAGACTGACA CCTCGTCCTC AGACTCGGAC
    GGCAGCGAGA GCCTGCCTCC CTCCTCACAG ACTCCTAAGT ACCCTGAAAG CAACAGGACT
    CCTGTCAAAC CCTCCTCAGT GGAGGAAGAA GATAGCTTTT TTCGGCAACG AATGTTCTCT
    CCTATGGAAG AGAAGGAGCT CCTTTCCCCA CTCAGTGAGC CTGATGACAG GTATCCACTC
    ATTGTGAAGA TTGACCTCAA TCTTCTGACT CGAATACCCG GGAAGCCTTA CAAAGAAACA
    GAGCCACCCA AGGGGGAAAA GAAAAATGTT CCAGAGAAAC ACTCGAGAGA GGTGCAGAAG
    CAAGCCTCAG AAAAGGCTTC CAACAAAGGC AAGAGGAAAC ACAAGAATGA TGATGATACC
    CGAGCCAGTG AGAGCAAAAA ACCCAAAACA GAGGACAAGA ACTCATCAGG CCACAAGCCA
    TCCAGCAGCA GAGAGTCATC TAAGCAGAGT AGTACAAAAG AAAAGGACTT GCTACCTTCA
    CCTGCTGGGC CCATTCTTTC AAAAGATTCA AAAACAGAGC ATGGTTCTCG GAAGAGGACT
    GTCAGTCAGT CGTCGTCCTT AAAGTCCAGT GGGACCAGCA GCAAAGAGAA CAGTGGCAGC
    AGCAGCAAGA GCAGCTCCTC CTCCACAGCG AAGCAGAAGA AGACTGAAGG CAAGGGTCCC
    AGCAGCTCCA AGGAGGCCAA GGAGAAGGCT CCAAATAGCT CCTCTAATTG TCCTCCGTCT
    ACACCAACTT CTGAGTCTTC GAAGCCTCGG AGAACAAAGC TTGCCTTTGA TGACAGAAAT
    TATTCAGCAG ACCATTATTT ACAAGAAGCA AAAAAACTCA AACACAATGC AGATGCTTTG
    TCTGACAGGT TTGAGAAAGC CGTGTACTAC CTTGATGCTG TAGTGTCTTT CATTGAGTGT
    GGGAATGCAT TAGAGAAGAA CGCTCAGGAA TCCAAGTCCC CATTCCCTAT GTACTCAGAC
    ACGGTGGAGC TCATTAAGTA CACTATGAAA CTAAAGAATT ATTTGGCACC AGATGCTACA
    GCTGCAGACA AGAGACTCAC AGTACTTTGC CTGAGATGCC AGTCATTATT GTACCTGAGG
    CTGTTCAAAC TGAAGAAGGA GAACGCTCTC AAGTACTCCA AGACGCTAAC TGAGCACCTG
    AAGAATTCTT ACAGTAATTC TCAAGCACCT TCACCCGGCT TGGGGAGCAA AGCTGTTGGG
    ATGCCCTCCC CTGTTTCTCC AAAACTGTCA CCAGGCAATT CTGGAAGTTA CTCTTCTGGG
    GGAAGTAGTG CTTCAGCAAG TGGTTCTTCA GTGACCATTC CACAGAAAAT CCACCAGATG
    GCAGCAAGCT ATGTCCAGGT TACATCTAAC TTCCTCTATG CCACAGAAAT TTGGGACCAA
    GCTGAACAGC TTTCCAAAGA ACAAAAAGAA TTCTTTGCTG AACTGGACAA AGTGATGGGC
    CCTCTCATCT TTAATGCAAG CATCATGACA GATCTGGCTC GTTACACCCG GCAGGGACTG
    CATTGGCTTC GGCAGGATGC CAAGCTGATA TCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001350260.1
    CDS368..3763
    Translation

    Target ORF information:

    RefSeq Version NM_001363331.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001363331.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA
    GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT
    GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA
    GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT
    CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG
    AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA
    AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG
    GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG
    GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC
    GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA
    TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT
    GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT
    GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG
    GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GTCGCAACAG
    TCTAACTTTG GCCCTGGAGA ACAAAAAAGA TACAGTACTG CTAAAACATC AAATGGACAC
    CAGTCAAAAT CTATGTTAAA AGATGACTTA AAACTAAGCA GCAGTGAAGA CAGTGATGGG
    GAACAGGATT GTGATAAGAC AATGCCAAGG AGTACACCAG GAAGTAACTC TGAACCTTCA
    CACCATAACA GTGAGGGAGC AGACAACTCC AGGGATGACT CGAGCAGCCA CAGTGGATCT
    GAGAGCAGCT CTGGGTCTGA CTCGGAGAGT GAGAGCAGCT CCAGTGACAG TGAGGCTAAT
    GAGCCGTCGC AGAGCGCATC TCCTGAGCCT GAACCACCAC CAACAAACAA GTGGCAACTT
    GATAATTGGT TGAATAAAGT GAACCCACAT AAAGTATCCC CAGCTTCCTC TGTAGACAGC
    AACATCCCAT CTTCTCAAGC CTACAAGAAA GAAGGCCGGG AGCAGGGCAC TGCCAGTAAC
    TACACAGATC CAGGGGGGAC AAAAGAAACG AGCTCTGCCA CCCCAGGACG AGACTCTAAA
    ACCATCCAAA AGGGATCAGA AAGTGGCCGT GGGAGGCAGA AGTCTCCTGC ACAGAGCGAC
    AGTACCACTC AGAGGAGAAC TGTCGGCAAA AAACAACCCA AAAAACCTGA GAAGTCAGCT
    GCTGAAGAGC CTCGTGGAGG CCTGAAGATA GAAAGTGAGA CCCCTGTGGA CATGGCTGCC
    AGCATGCCCT CCAGCAGGCA CAAAGCAGCC ACCAAGGGCT CGAGGAAACC CAACATCAAA
    AAGGAATCAA AATCTTCTCC TCGACCTACG GCAGAGAAGA AGAAGTACAA GTCAGCCAGT
    AAACCGTCCC AGAAGTCTCG GGAGATCATA GAGACTGACA CCTCGTCCTC AGACTCGGAC
    GGCAGCGAGA GCCTGCCTCC CTCCTCACAG ACTCCTAAGT ACCCTGAAAG CAACAGGACT
    CCTGTCAAAC CCTCCTCAGT GGAGGAAGAA GATAGCTTTT TTCGGCAACG AATGTTCTCT
    CCTATGGAAG AGAAGGAGCT CCTTTCCCCA CTCAGTGAGC CTGATGACAG GTATCCACTC
    ATTGTGAAGA TTGACCTCAA TCTTCTGACT CGAATACCCG GGAAGCCTTA CAAAGAAACA
    GAGCCACCCA AGGGGGAAAA GAAAAATGTT CCAGAGAAAC ACTCGAGAGA GGTGCAGAAG
    CAAGCCTCAG AAAAGGCTTC CAACAAAGGC AAGAGGAAAC ACAAGAATGA TGATGATACC
    CGAGCCAGTG AGAGCAAAAA ACCCAAAACA GAGGACAAGA ACTCATCAGG CCACAAGCCA
    TCCAGCAGCA GAGAGTCATC TAAGCAGAGT AGTACAAAAG AAAAGGACTT GCTACCTTCA
    CCTGCTGGGC CCATTCTTTC AAAAGATTCA AAAACAGAGC ATGGTTCTCG GAAGAGGACT
    GTCAGTCAGT CGTCGTCCTT AAAGTCCAGT GGGACCAGCA GCAAAGAGAA CAGTGGCAGC
    AGCAGCAAGA GCAGCTCCTC CTCCACAGCG AAGCAGAAGA AGACTGAAGG CAAGGGTCCC
    AGCAGCTCCA AGGAGGCCAA GGAGAAGGCT CCAAATAGCT CCTCTAATTG TCCTCCGTCT
    ACACCAACTT CTGAGTCTTC GAAGCCTCGG AGAACAAAGC TTGCCTTTGA TGACAGAAAT
    TATTCAGCAG ACCATTATTT ACAAGAAGCA AAAAAACTCA AACACAATGC AGATGCTTTG
    TCTGACAGGT TTGAGAAAGC CGTGTACTAC CTTGATGCTG TAGTGTCTTT CATTGAGTGT
    GGGAATGCAT TAGAGAAGAA CGCTCAGGAA TCCAAGTCCC CATTCCCTAT GTACTCAGAC
    ACGGTGGAGC TCATTAAGTA CACTATGAAA CTAAAGAATT ATTTGGCACC AGATGCTACA
    GCTGCAGACA AGAGACTCAC AGTACTTTGC CTGAGATGCC AGTCATTATT GTACCTGAGG
    CTGTTCAAAC TGAAGAAGGA GAACGCTCTC AAGTACTCCA AGACGCTAAC TGAGCACCTG
    AAGAATTCTT ACAGTAATTC TCAAGCACCT TCACCCGGCT TGGGGAGCAA AGCTGTTGGG
    ATGCCCTCCC CTGTTTCTCC AAAACTGTCA CCAGGCAATT CTGGAAGTTA CTCTTCTGGG
    GGAAGTAGTG CTTCAGCAAG TGGTTCTTCA GTGACCATTC CACAGAAAAT CCACCAGATG
    GCAGCAAGCT ATGTCCAGGT TACATCTAAC TTCCTCTATG CCACAGAAAT TTGGGACCAA
    GCTGAACAGC TTTCCAAAGA ACAAAAAGAA TTCTTTGCTG AACTGGACAA AGTGATGGGC
    CCTCTCATCT TTAATGCAAG CATCATGACA GATCTGGCTC GTTACACCCG GCAGGGACTG
    CATTGGCTTC GGCAGGATGC CAAGCTGATA TCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu15411
    Clone ID Related Accession (Same CDS sequence) XM_011249331.3 , NM_033565.2 , XM_011249331.2
    Accession Version XM_011249331.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product AF4/FMR2 family member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249331.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA
    GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT
    GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA
    GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT
    CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG
    AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA
    AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG
    GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG
    GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC
    GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA
    TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT
    GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT
    GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG
    GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GATGACGCAT
    TCATGGCCTC CCCCTCTAAC GGCTATTCAT ACACCATGCA AAACAGAACC TTCCAAATTC
    CCTTTTCCAA CTAAGGAGTC GCAACAGTCT AACTTTGGCC CTGGAGAACA AAAAAGATAC
    AGTACTGCTA AAACATCAAA TGGACACCAG TCAAAATCTA TGTTAAAAGA TGACTTAAAA
    CTAAGCAGCA GTGAAGACAG TGATGGGGAA CAGGATTGTG ATAAGACAAT GCCAAGGAGT
    ACACCAGGAA GTAACTCTGA ACCTTCACAC CATAACAGTG AGGGAGCAGA CAACTCCAGG
    GATGACTCGA GCAGCCACAG TGGATCTGAG AGCAGCTCTG GGTCTGACTC GGAGAGTGAG
    AGCAGCTCCA GTGACAGTGA GGCTAATGAG CCGTCGCAGA GCGCATCTCC TGAGCCTGAA
    CCACCACCAA CAAACAAGTG GCAACTTGAT AATTGGTTGA ATAAAGTGAA CCCACATAAA
    GTATCCCCAG CTTCCTCTGT AGACAGCAAC ATCCCATCTT CTCAAGCCTA CAAGAAAGAA
    GGCCGGGAGC AGGGCACTGC CAGTAACTAC ACAGATCCAG GGGGGACAAA AGAAACGAGC
    TCTGCCACCC CAGGACGAGA CTCTAAAACC ATCCAAAAGG GATCAGAAAG TGGCCGTGGG
    AGGCAGAAGT CTCCTGCACA GAGCGACAGT ACCACTCAGA GGAGAACTGT CGGCAAAAAA
    CAACCCAAAA AACCTGAGAA GTCAGCTGCT GAAGAGCCTC GTGGAGGCCT GAAGATAGAA
    AGTGAGACCC CTGTGGACAT GGCTGCCAGC ATGCCCTCCA GCAGGCACAA AGCAGCCACC
    AAGGGCTCGA GGAAACCCAA CATCAAAAAG GAATCAAAAT CTTCTCCTCG ACCTACGGCA
    GAGAAGAAGA AGTACAAGTC AGCCAGTAAA CCGTCCCAGA AGTCTCGGGA GATCATAGAG
    ACTGACACCT CGTCCTCAGA CTCGGACGGC AGCGAGAGCC TGCCTCCCTC CTCACAGACT
    CCTAAGTACC CTGAAAGCAA CAGGACTCCT GTCAAACCCT CCTCAGTGGA GGAAGAAGAT
    AGCTTTTTTC GGCAACGAAT GTTCTCTCCT ATGGAAGAGA AGGAGCTCCT TTCCCCACTC
    AGTGAGCCTG ATGACAGGTA TCCACTCATT GTGAAGATTG ACCTCAATCT TCTGACTCGA
    ATACCCGGGA AGCCTTACAA AGAAACAGAG CCACCCAAGG GGGAAAAGAA AAATGTTCCA
    GAGAAACACT CGAGAGAGGT GCAGAAGCAA GCCTCAGAAA AGGCTTCCAA CAAAGGCAAG
    AGGAAACACA AGAATGATGA TGATACCCGA GCCAGTGAGA GCAAAAAACC CAAAACAGAG
    GACAAGAACT CATCAGGCCA CAAGCCATCC AGCAGCAGAG AGTCATCTAA GCAGAGTAGT
    ACAAAAGAAA AGGACTTGCT ACCTTCACCT GCTGGGCCCA TTCTTTCAAA AGATTCAAAA
    ACAGAGCATG GTTCTCGGAA GAGGACTGTC AGTCAGTCGT CGTCCTTAAA GTCCAGTGGG
    ACCAGCAGCA AAGAGAACAG TGGCAGCAGC AGCAAGAGCA GCTCCTCCTC CACAGCGAAG
    CAGAAGAAGA CTGAAGGCAA GGGTCCCAGC AGCTCCAAGG AGGCCAAGGA GAAGGCTCCA
    AATAGCTCCT CTAATTGTCC TCCGTCTACA CCAACTTCTG AGTCTTCGAA GCCTCGGAGA
    ACAAAGCTTG CCTTTGATGA CAGAAATTAT TCAGCAGACC ATTATTTACA AGAAGCAAAA
    AAACTCAAAC ACAATGCAGA TGCTTTGTCT GACAGGTTTG AGAAAGCCGT GTACTACCTT
    GATGCTGTAG TGTCTTTCAT TGAGTGTGGG AATGCATTAG AGAAGAACGC TCAGGAATCC
    AAGTCCCCAT TCCCTATGTA CTCAGACACG GTGGAGCTCA TTAAGTACAC TATGAAACTA
    AAGAATTATT TGGCACCAGA TGCTACAGCT GCAGACAAGA GACTCACAGT ACTTTGCCTG
    AGATGCCAGT CATTATTGTA CCTGAGGCTG TTCAAACTGA AGAAGGAGAA CGCTCTCAAG
    TACTCCAAGA CGCTAACTGA GCACCTGAAG AATTCTTACA GTAATTCTCA AGCACCTTCA
    CCCGGCTTGG GGAGCAAAGC TGTTGGGATG CCCTCCCCTG TTTCTCCAAA ACTGTCACCA
    GGCAATTCTG GAAGTTACTC TTCTGGGGGA AGTAGTGCTT CAGCAAGTGG TTCTTCAGTG
    ACCATTCCAC AGAAAATCCA CCAGATGGCA GCAAGCTATG TCCAGGTTAC ATCTAACTTC
    CTCTATGCCA CAGAAATTTG GGACCAAGCT GAACAGCTTT CCAAAGAACA AAAAGAATTC
    TTTGCTGAAC TGGACAAAGT GATGGGCCCT CTCATCTTTA ATGCAAGCAT CATGACAGAT
    CTGGCTCGTT ACACCCGGCA GGGACTGCAT TGGCTTCGGC AGGATGCCAA GCTGATATCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011247633.1
    CDS745..4227
    Translation

    Target ORF information:

    RefSeq Version XM_011249331.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011249331.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA
    GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT
    GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA
    GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT
    CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG
    AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA
    AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG
    GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG
    GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC
    GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA
    TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT
    GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT
    GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG
    GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GATGACGCAT
    TCATGGCCTC CCCCTCTAAC GGCTATTCAT ACACCATGCA AAACAGAACC TTCCAAATTC
    CCTTTTCCAA CTAAGGAGTC GCAACAGTCT AACTTTGGCC CTGGAGAACA AAAAAGATAC
    AGTACTGCTA AAACATCAAA TGGACACCAG TCAAAATCTA TGTTAAAAGA TGACTTAAAA
    CTAAGCAGCA GTGAAGACAG TGATGGGGAA CAGGATTGTG ATAAGACAAT GCCAAGGAGT
    ACACCAGGAA GTAACTCTGA ACCTTCACAC CATAACAGTG AGGGAGCAGA CAACTCCAGG
    GATGACTCGA GCAGCCACAG TGGATCTGAG AGCAGCTCTG GGTCTGACTC GGAGAGTGAG
    AGCAGCTCCA GTGACAGTGA GGCTAATGAG CCGTCGCAGA GCGCATCTCC TGAGCCTGAA
    CCACCACCAA CAAACAAGTG GCAACTTGAT AATTGGTTGA ATAAAGTGAA CCCACATAAA
    GTATCCCCAG CTTCCTCTGT AGACAGCAAC ATCCCATCTT CTCAAGCCTA CAAGAAAGAA
    GGCCGGGAGC AGGGCACTGC CAGTAACTAC ACAGATCCAG GGGGGACAAA AGAAACGAGC
    TCTGCCACCC CAGGACGAGA CTCTAAAACC ATCCAAAAGG GATCAGAAAG TGGCCGTGGG
    AGGCAGAAGT CTCCTGCACA GAGCGACAGT ACCACTCAGA GGAGAACTGT CGGCAAAAAA
    CAACCCAAAA AACCTGAGAA GTCAGCTGCT GAAGAGCCTC GTGGAGGCCT GAAGATAGAA
    AGTGAGACCC CTGTGGACAT GGCTGCCAGC ATGCCCTCCA GCAGGCACAA AGCAGCCACC
    AAGGGCTCGA GGAAACCCAA CATCAAAAAG GAATCAAAAT CTTCTCCTCG ACCTACGGCA
    GAGAAGAAGA AGTACAAGTC AGCCAGTAAA CCGTCCCAGA AGTCTCGGGA GATCATAGAG
    ACTGACACCT CGTCCTCAGA CTCGGACGGC AGCGAGAGCC TGCCTCCCTC CTCACAGACT
    CCTAAGTACC CTGAAAGCAA CAGGACTCCT GTCAAACCCT CCTCAGTGGA GGAAGAAGAT
    AGCTTTTTTC GGCAACGAAT GTTCTCTCCT ATGGAAGAGA AGGAGCTCCT TTCCCCACTC
    AGTGAGCCTG ATGACAGGTA TCCACTCATT GTGAAGATTG ACCTCAATCT TCTGACTCGA
    ATACCCGGGA AGCCTTACAA AGAAACAGAG CCACCCAAGG GGGAAAAGAA AAATGTTCCA
    GAGAAACACT CGAGAGAGGT GCAGAAGCAA GCCTCAGAAA AGGCTTCCAA CAAAGGCAAG
    AGGAAACACA AGAATGATGA TGATACCCGA GCCAGTGAGA GCAAAAAACC CAAAACAGAG
    GACAAGAACT CATCAGGCCA CAAGCCATCC AGCAGCAGAG AGTCATCTAA GCAGAGTAGT
    ACAAAAGAAA AGGACTTGCT ACCTTCACCT GCTGGGCCCA TTCTTTCAAA AGATTCAAAA
    ACAGAGCATG GTTCTCGGAA GAGGACTGTC AGTCAGTCGT CGTCCTTAAA GTCCAGTGGG
    ACCAGCAGCA AAGAGAACAG TGGCAGCAGC AGCAAGAGCA GCTCCTCCTC CACAGCGAAG
    CAGAAGAAGA CTGAAGGCAA GGGTCCCAGC AGCTCCAAGG AGGCCAAGGA GAAGGCTCCA
    AATAGCTCCT CTAATTGTCC TCCGTCTACA CCAACTTCTG AGTCTTCGAA GCCTCGGAGA
    ACAAAGCTTG CCTTTGATGA CAGAAATTAT TCAGCAGACC ATTATTTACA AGAAGCAAAA
    AAACTCAAAC ACAATGCAGA TGCTTTGTCT GACAGGTTTG AGAAAGCCGT GTACTACCTT
    GATGCTGTAG TGTCTTTCAT TGAGTGTGGG AATGCATTAG AGAAGAACGC TCAGGAATCC
    AAGTCCCCAT TCCCTATGTA CTCAGACACG GTGGAGCTCA TTAAGTACAC TATGAAACTA
    AAGAATTATT TGGCACCAGA TGCTACAGCT GCAGACAAGA GACTCACAGT ACTTTGCCTG
    AGATGCCAGT CATTATTGTA CCTGAGGCTG TTCAAACTGA AGAAGGAGAA CGCTCTCAAG
    TACTCCAAGA CGCTAACTGA GCACCTGAAG AATTCTTACA GTAATTCTCA AGCACCTTCA
    CCCGGCTTGG GGAGCAAAGC TGTTGGGATG CCCTCCCCTG TTTCTCCAAA ACTGTCACCA
    GGCAATTCTG GAAGTTACTC TTCTGGGGGA AGTAGTGCTT CAGCAAGTGG TTCTTCAGTG
    ACCATTCCAC AGAAAATCCA CCAGATGGCA GCAAGCTATG TCCAGGTTAC ATCTAACTTC
    CTCTATGCCA CAGAAATTTG GGACCAAGCT GAACAGCTTT CCAAAGAACA AAAAGAATTC
    TTTGCTGAAC TGGACAAAGT GATGGGCCCT CTCATCTTTA ATGCAAGCAT CATGACAGAT
    CTGGCTCGTT ACACCCGGCA GGGACTGCAT TGGCTTCGGC AGGATGCCAA GCTGATATCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu47156
    Clone ID Related Accession (Same CDS sequence) NM_001363331.1 , XM_006534560.3 , XM_030246443.1
    Accession Version XM_006534560.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product AF4/FMR2 family member 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534560.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA
    GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT
    GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA
    GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT
    CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG
    AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA
    AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG
    GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG
    GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC
    GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA
    TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT
    GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT
    GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG
    GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GTCGCAACAG
    TCTAACTTTG GCCCTGGAGA ACAAAAAAGA TACAGTACTG CTAAAACATC AAATGGACAC
    CAGTCAAAAT CTATGTTAAA AGATGACTTA AAACTAAGCA GCAGTGAAGA CAGTGATGGG
    GAACAGGATT GTGATAAGAC AATGCCAAGG AGTACACCAG GAAGTAACTC TGAACCTTCA
    CACCATAACA GTGAGGGAGC AGACAACTCC AGGGATGACT CGAGCAGCCA CAGTGGATCT
    GAGAGCAGCT CTGGGTCTGA CTCGGAGAGT GAGAGCAGCT CCAGTGACAG TGAGGCTAAT
    GAGCCGTCGC AGAGCGCATC TCCTGAGCCT GAACCACCAC CAACAAACAA GTGGCAACTT
    GATAATTGGT TGAATAAAGT GAACCCACAT AAAGTATCCC CAGCTTCCTC TGTAGACAGC
    AACATCCCAT CTTCTCAAGC CTACAAGAAA GAAGGCCGGG AGCAGGGCAC TGCCAGTAAC
    TACACAGATC CAGGGGGGAC AAAAGAAACG AGCTCTGCCA CCCCAGGACG AGACTCTAAA
    ACCATCCAAA AGGGATCAGA AAGTGGCCGT GGGAGGCAGA AGTCTCCTGC ACAGAGCGAC
    AGTACCACTC AGAGGAGAAC TGTCGGCAAA AAACAACCCA AAAAACCTGA GAAGTCAGCT
    GCTGAAGAGC CTCGTGGAGG CCTGAAGATA GAAAGTGAGA CCCCTGTGGA CATGGCTGCC
    AGCATGCCCT CCAGCAGGCA CAAAGCAGCC ACCAAGGGCT CGAGGAAACC CAACATCAAA
    AAGGAATCAA AATCTTCTCC TCGACCTACG GCAGAGAAGA AGAAGTACAA GTCAGCCAGT
    AAACCGTCCC AGAAGTCTCG GGAGATCATA GAGACTGACA CCTCGTCCTC AGACTCGGAC
    GGCAGCGAGA GCCTGCCTCC CTCCTCACAG ACTCCTAAGT ACCCTGAAAG CAACAGGACT
    CCTGTCAAAC CCTCCTCAGT GGAGGAAGAA GATAGCTTTT TTCGGCAACG AATGTTCTCT
    CCTATGGAAG AGAAGGAGCT CCTTTCCCCA CTCAGTGAGC CTGATGACAG GTATCCACTC
    ATTGTGAAGA TTGACCTCAA TCTTCTGACT CGAATACCCG GGAAGCCTTA CAAAGAAACA
    GAGCCACCCA AGGGGGAAAA GAAAAATGTT CCAGAGAAAC ACTCGAGAGA GGTGCAGAAG
    CAAGCCTCAG AAAAGGCTTC CAACAAAGGC AAGAGGAAAC ACAAGAATGA TGATGATACC
    CGAGCCAGTG AGAGCAAAAA ACCCAAAACA GAGGACAAGA ACTCATCAGG CCACAAGCCA
    TCCAGCAGCA GAGAGTCATC TAAGCAGAGT AGTACAAAAG AAAAGGACTT GCTACCTTCA
    CCTGCTGGGC CCATTCTTTC AAAAGATTCA AAAACAGAGC ATGGTTCTCG GAAGAGGACT
    GTCAGTCAGT CGTCGTCCTT AAAGTCCAGT GGGACCAGCA GCAAAGAGAA CAGTGGCAGC
    AGCAGCAAGA GCAGCTCCTC CTCCACAGCG AAGCAGAAGA AGACTGAAGG CAAGGGTCCC
    AGCAGCTCCA AGGAGGCCAA GGAGAAGGCT CCAAATAGCT CCTCTAATTG TCCTCCGTCT
    ACACCAACTT CTGAGTCTTC GAAGCCTCGG AGAACAAAGC TTGCCTTTGA TGACAGAAAT
    TATTCAGCAG ACCATTATTT ACAAGAAGCA AAAAAACTCA AACACAATGC AGATGCTTTG
    TCTGACAGGT TTGAGAAAGC CGTGTACTAC CTTGATGCTG TAGTGTCTTT CATTGAGTGT
    GGGAATGCAT TAGAGAAGAA CGCTCAGGAA TCCAAGTCCC CATTCCCTAT GTACTCAGAC
    ACGGTGGAGC TCATTAAGTA CACTATGAAA CTAAAGAATT ATTTGGCACC AGATGCTACA
    GCTGCAGACA AGAGACTCAC AGTACTTTGC CTGAGATGCC AGTCATTATT GTACCTGAGG
    CTGTTCAAAC TGAAGAAGGA GAACGCTCTC AAGTACTCCA AGACGCTAAC TGAGCACCTG
    AAGAATTCTT ACAGTAATTC TCAAGCACCT TCACCCGGCT TGGGGAGCAA AGCTGTTGGG
    ATGCCCTCCC CTGTTTCTCC AAAACTGTCA CCAGGCAATT CTGGAAGTTA CTCTTCTGGG
    GGAAGTAGTG CTTCAGCAAG TGGTTCTTCA GTGACCATTC CACAGAAAAT CCACCAGATG
    GCAGCAAGCT ATGTCCAGGT TACATCTAAC TTCCTCTATG CCACAGAAAT TTGGGACCAA
    GCTGAACAGC TTTCCAAAGA ACAAAAAGAA TTCTTTGCTG AACTGGACAA AGTGATGGGC
    CCTCTCATCT TTAATGCAAG CATCATGACA GATCTGGCTC GTTACACCCG GCAGGGACTG
    CATTGGCTTC GGCAGGATGC CAAGCTGATA TCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006534623.1
    CDS653..4048
    Translation

    Target ORF information:

    RefSeq Version XM_006534560.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus AF4/FMR2 family, member 4 (Aff4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006534560.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACCGTG AAGACCGGAA TGTGCTGCGC ATGAAAGAAA GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GTGAAGACGC CTTCCCACCT AGTTCTCCTC TCTTTGCTGA GCCATACAAA
    GTCACTAGCA AAGAAGATAA GTTATCAAGT CGGATTCAGA GCATGCTTGG AAACTACGAT
    GAAATGAAGG ATTACATAGG AGACAGATCG ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    GCAGTACCAA CGACCACAGA CGAGAAAGCA AACCCAAATT TTTTTGAGCA GAGACATGGA
    GGATCTCATC AGAGTAGCAA ATGGACTCCT GTAGGCCCCG CCCCCAGCAC CTCTCAGTCT
    CAGAAACGCT CCTCTGCCCT GCAGAGTGGA CACAGCAGCC AACGGAGTGG TGCAGGAGGG
    AGTGGCGCCA GCAGCAGTGG CCAGAGGCAT GACCGTGACT CCTACAGCAG TAGCAGGAAA
    AAAGGCCAGC ACGGCTCAGA GCACTCCAAA TCTCGATCCT CAAGCCCTGG AAAACCCCAG
    GCTGTTTCTT CATTAAGTTC TAGTCATTCC AGGTCTCATG GGAATGATCA CCATAGCAAG
    GAACATCAGC GCTCCAAATC ACCTCGGGAC CCTGATGCCA ACTGGGATTC TCCTTCCCGC
    GGACCTTTTT CAAGTGGGCA ACATTCAAGT CAGTCTTTCC CACCTTCTTT GATGTCAAAA
    TCTAGTTCAA TGTTACAGAA ACCCACTGCC TATGTACGGC CCATGGATGG ACAGGAGTCT
    GTGGAACCAA AGCTGTCCTC TGAGCACTAC AGCAGCCAGT CTCATGGTAA TAGCATGACT
    GAGCTGAAGC CCAGCAGCAA AGCACATCTC ACCAAGCTGA AAATTCCTTC CCGACCTTTG
    GATGCATCGG TTTCTGGTGA TGTAAGCTGT GTAGATGAAA TACTTAAAGA GTCGCAACAG
    TCTAACTTTG GCCCTGGAGA ACAAAAAAGA TACAGTACTG CTAAAACATC AAATGGACAC
    CAGTCAAAAT CTATGTTAAA AGATGACTTA AAACTAAGCA GCAGTGAAGA CAGTGATGGG
    GAACAGGATT GTGATAAGAC AATGCCAAGG AGTACACCAG GAAGTAACTC TGAACCTTCA
    CACCATAACA GTGAGGGAGC AGACAACTCC AGGGATGACT CGAGCAGCCA CAGTGGATCT
    GAGAGCAGCT CTGGGTCTGA CTCGGAGAGT GAGAGCAGCT CCAGTGACAG TGAGGCTAAT
    GAGCCGTCGC AGAGCGCATC TCCTGAGCCT GAACCACCAC CAACAAACAA GTGGCAACTT
    GATAATTGGT TGAATAAAGT GAACCCACAT AAAGTATCCC CAGCTTCCTC TGTAGACAGC
    AACATCCCAT CTTCTCAAGC CTACAAGAAA GAAGGCCGGG AGCAGGGCAC TGCCAGTAAC
    TACACAGATC CAGGGGGGAC AAAAGAAACG AGCTCTGCCA CCCCAGGACG AGACTCTAAA
    ACCATCCAAA AGGGATCAGA AAGTGGCCGT GGGAGGCAGA AGTCTCCTGC ACAGAGCGAC
    AGTACCACTC AGAGGAGAAC TGTCGGCAAA AAACAACCCA AAAAACCTGA GAAGTCAGCT
    GCTGAAGAGC CTCGTGGAGG CCTGAAGATA GAAAGTGAGA CCCCTGTGGA CATGGCTGCC
    AGCATGCCCT CCAGCAGGCA CAAAGCAGCC ACCAAGGGCT CGAGGAAACC CAACATCAAA
    AAGGAATCAA AATCTTCTCC TCGACCTACG GCAGAGAAGA AGAAGTACAA GTCAGCCAGT
    AAACCGTCCC AGAAGTCTCG GGAGATCATA GAGACTGACA CCTCGTCCTC AGACTCGGAC
    GGCAGCGAGA GCCTGCCTCC CTCCTCACAG ACTCCTAAGT ACCCTGAAAG CAACAGGACT
    CCTGTCAAAC CCTCCTCAGT GGAGGAAGAA GATAGCTTTT TTCGGCAACG AATGTTCTCT
    CCTATGGAAG AGAAGGAGCT CCTTTCCCCA CTCAGTGAGC CTGATGACAG GTATCCACTC
    ATTGTGAAGA TTGACCTCAA TCTTCTGACT CGAATACCCG GGAAGCCTTA CAAAGAAACA
    GAGCCACCCA AGGGGGAAAA GAAAAATGTT CCAGAGAAAC ACTCGAGAGA GGTGCAGAAG
    CAAGCCTCAG AAAAGGCTTC CAACAAAGGC AAGAGGAAAC ACAAGAATGA TGATGATACC
    CGAGCCAGTG AGAGCAAAAA ACCCAAAACA GAGGACAAGA ACTCATCAGG CCACAAGCCA
    TCCAGCAGCA GAGAGTCATC TAAGCAGAGT AGTACAAAAG AAAAGGACTT GCTACCTTCA
    CCTGCTGGGC CCATTCTTTC AAAAGATTCA AAAACAGAGC ATGGTTCTCG GAAGAGGACT
    GTCAGTCAGT CGTCGTCCTT AAAGTCCAGT GGGACCAGCA GCAAAGAGAA CAGTGGCAGC
    AGCAGCAAGA GCAGCTCCTC CTCCACAGCG AAGCAGAAGA AGACTGAAGG CAAGGGTCCC
    AGCAGCTCCA AGGAGGCCAA GGAGAAGGCT CCAAATAGCT CCTCTAATTG TCCTCCGTCT
    ACACCAACTT CTGAGTCTTC GAAGCCTCGG AGAACAAAGC TTGCCTTTGA TGACAGAAAT
    TATTCAGCAG ACCATTATTT ACAAGAAGCA AAAAAACTCA AACACAATGC AGATGCTTTG
    TCTGACAGGT TTGAGAAAGC CGTGTACTAC CTTGATGCTG TAGTGTCTTT CATTGAGTGT
    GGGAATGCAT TAGAGAAGAA CGCTCAGGAA TCCAAGTCCC CATTCCCTAT GTACTCAGAC
    ACGGTGGAGC TCATTAAGTA CACTATGAAA CTAAAGAATT ATTTGGCACC AGATGCTACA
    GCTGCAGACA AGAGACTCAC AGTACTTTGC CTGAGATGCC AGTCATTATT GTACCTGAGG
    CTGTTCAAAC TGAAGAAGGA GAACGCTCTC AAGTACTCCA AGACGCTAAC TGAGCACCTG
    AAGAATTCTT ACAGTAATTC TCAAGCACCT TCACCCGGCT TGGGGAGCAA AGCTGTTGGG
    ATGCCCTCCC CTGTTTCTCC AAAACTGTCA CCAGGCAATT CTGGAAGTTA CTCTTCTGGG
    GGAAGTAGTG CTTCAGCAAG TGGTTCTTCA GTGACCATTC CACAGAAAAT CCACCAGATG
    GCAGCAAGCT ATGTCCAGGT TACATCTAAC TTCCTCTATG CCACAGAAAT TTGGGACCAA
    GCTGAACAGC TTTCCAAAGA ACAAAAAGAA TTCTTTGCTG AACTGGACAA AGTGATGGGC
    CCTCTCATCT TTAATGCAAG CATCATGACA GATCTGGCTC GTTACACCCG GCAGGGACTG
    CATTGGCTTC GGCAGGATGC CAAGCTGATA TCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.