AFF4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	AFF4
Entrez Gene ID	541306
Full Name	AF4/FMR2 family member 4
General protein information	Preferred Names AF4/FMR2 family member 4 Names AF4/FMR2 family member 4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001191129.1	NP_001178058.1	AF4/FMR2 family member 4
XM_005209304.4	XP_005209361.1	AF4/FMR2 family member 4 isoform X1
XM_005209304.3	XP_005209361.1	AF4/FMR2 family member 4 isoform X1
XM_010807254.2	XP_010805556.1	AF4/FMR2 family member 4 isoform X2
XM_024994567.1	XP_024850335.1	AF4/FMR2 family member 4 isoform X1
XM_010807254.3	XP_010805556.1	AF4/FMR2 family member 4 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AFF4	XP_517928.2
Canis lupus familiaris (dog)	AFF4	XP_850309.1
Mus musculus (house mouse)	Aff4	NP_291043.1
Gallus gallus (chicken)	AFF4	XP_004945088.1
Danio rerio (zebrafish)	aff4	XP_684327.6
Rattus norvegicus (Norway rat)	Aff4	NP_001100471.1
Xenopus tropicalis (tropical clawed frog)	aff4	XP_002935317.2
Homo sapiens (human)	AFF4	NP_055238.1
Bos taurus (cattle)	AFF4	NP_001178058.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AFF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa21434	NM_001191129.1 Latest version!	Bos taurus AF4/FMR2 family member 4 (AFF4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa21434	XM_024994567.1 Latest version!	Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa21434	XM_005209304.4 Latest version!	Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa21435	XM_010807254.3 Latest version!	Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa21434	XM_005209304.3	Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa21435	XM_010807254.2	Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFF4 ( NM_001191129.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178058.1 Bos taurus AF4/FMR2 family member 4 (AFF4), mRNA.

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CloneID	OBa21434
Clone ID Related Accession (Same CDS sequence)	NM_001191129.1 , XM_005209304.4 , XM_005209304.3 , XM_024994567.1
Accession Version	NM_001191129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000007.1. On Jul 17, 2010 this sequence version replaced XM_001249664.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR6764281.23887.1, SRR5571260.50474.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG 
AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT 
CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC 
AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG 
AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT 
TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT 
GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT 
AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA 
GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAGTTT TGGCCCTGGA 
GAACAAAAAA GATATAATCC TTCTTCTAAA ACTTCAAATG CACACCAGTC TAAATCTATG 
TTAAAAGATG ACTTAAAGCT AAGCAGCAGT GAAGACAGTG ATGGAGAACA GGATTGTGAT 
AAGACAATGC CAAGGAGTAC ACCAGGAAGT AACTCTGAAC CTTCACACCA TAATAGTGAA 
GGGGCAGATA ACTCCAGGGA TGATTCTAGC AGCCACAGTG GATCTGAAAG CAGCTCTGGA 
TCTGACTCAG AGAGTGAAAG TAGTTCCAGT GACAGTGAGG CAAATGAGCC ATCCCAGAGT 
GCATCTCCTG AGCCTGAACC ACCTCCCACA AATAAATGGC AACTTGATAA CTGGCTGAAT 
AAAGTAAATC CACATAAAGT ATCACCAGCT TCTTCTGTGG ACAGTAATAT CCCATCATCT 
CAAGGCTATA AAAAGGAAGG CCGAGAACAG GGCACAGGGA ATAGCTACAC AGATCCAGGT 
GGTCCTAAAG AAACAAGTTC TGCTACTCCT GGACGAGACT CCAAAACTGT CCAAAAGGGA 
TCAGAAAGTG GGCGTGGGAG GCAAAAGTCT CCTGCCCAGA GTGACAGCAC AGCACAGAGG 
AGAACTGTAG GGAAAAAACA ACCCAAAAAG ACTGAGAAGG CAGCTGCTGA AGAGCCCCGT 
GGAGGCCTGA AGATAGAGAG TGAAACTCCT GTAGACATGG CTACCAGCAT GCCCTCCAGC 
AGACACAAAG CGGCCACCAA AGGCTCAAGA AAACCCAATA TAAAAAAGGA GTCCAAATCT 
TCCCCTCGAC CTTCAGCAGA GAAAAAGAAA TACAAGTCAA CAAGTAAATC TTCCCAGAAA 
TCAAGGGAAA TTATAGAAAC AGATACCTCA TCCTCAGATT CGGATGAAAG CGAGAGCCTT 
CCTCCTTCCT CACAAACTCC TAAGTACCCT GAGAGTAATA GGACTCCCGT TAAACCCTCC 
TCAGTGGAGG AAGAAGATAG CTTTTTTCGG CAACGAATGT TCTCTCCTAT GGAAGAGAAG 
GAACTTCTTT CACCCCTCAG TGAGCCTGAT GACAGGTATC CACTTATTGT GAAGATTGAC 
CTGAATCTCT TGACTAGAAT ACCAGGAAAG CCTTACAAAG AAACAGAGCC ACCCAAGGGG 
GAAAAGAAAA ATGTGCCAGA AAAGCACACA AGAGAGGCTC AGAAACAAGC CTCAGAGAAA 
GTTTCCAACA AAGGCAAGAG GAAACATAAG AATGAAGATG ATAACCGAGC CAATGAAAGC 
AAGAAACCTA AAACAGAGGA CAAGAATATG GCAGGCCACA AGCCATCCAG CAACAAAGAG 
TCATCTAAAC AGAGTGCTTC TAAAGAAAAA GATTTATTGC CTTCTCCTGC TGGGCCTGTT 
CCTTCAAAAG ATCCAAAAAC AGAGCATGGC TCTCGGAAGA GGACTGTTAG TCAGTCTTCT 
TCCTTAAAGT CAAGTAATAA CAGCAACAAG GAAAATAGTG CCAGCAGCAA AAACAGTTCC 
TCCGCATCAA AGCAGAAGAG GACTGAAGGG AAGACTTCCA GTAGCTCTAA GGAGGTCAAG 
GAAAAAGCTC CAAATAGTTC TTCTAACTGT CCTCCATCTG CACCAACTCC TGATGCTTCT 
AAGCCTCGGA GAACGAAGCT TGCCTTTGAT GACAGAAATT ATTCAGCAGA CCATTATTTA 
CAAGAAGCAA AAAAGCTAAA GCACAATGCA GATGCATTGT CTGATAGGTT TGAGAAAGCT 
GTATACTATC TTGATGCTGT GGTATCTTTC ATTGAATGTG GGAATGCATT AGAGAAAAAT 
GCTCAGGAAT CCAAATCCCC ATTCCTTATG TATTCAGAAA CGGTGGAGCT CATCAAATAC 
ACTATGAAGC TAAAGAATTA CTTGGCACCA GATGCTACAG CTGCAGATAA AAGACTTACA 
GTGCTTTGCC TGCGATGCCA GTCTTTGCTG TACCTGAGGC TCTTCAAACT GAAGAAAGAA 
ACTGCTCTGA AGTATTCAAA GACACTGACA GAGCATCTGA AGAATTCTTA CAATAACTCT 
CAAGCACCAT CACCAGGCTT GGGAAGCAAA GCTGTTGGGA TGCCTTCCCC TGTTTCTCCA 
AAGCTGTCAC CAGGCAATTC AGGAAATTAT TCTTCTGGAG CCAGTAGTGC TTCAGCAAGT 
GGTTCTTCAG TGACCATTCC ACAGAGGATT CACCACATGG CAGCCAGCTA TGTTCAGGTT 
ACATCCAACT TCCTCTATGC CACTGAAATT TGGGACCAAG CTGAACAGCT TTCCAAAGAG 
CAAAAAGAAT TCTTTGCTGA ACTGGATAAA GTAATGGGCC CTCTCATCTT TAATGCAAGC 
ATCATGACAG ACCTAGTTCG TTACACCCGG CAGGGACTGC ACTGGCTTCG CCAGGATGCC 
AAGTTGATAT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178058.1
CDS	170..3664
Translation	MNREDRNVLRMKERERRNQEIQQGDDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPTADEKSNPNFFEQRHGSSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRASAGASGGTNSSGQRHDRDSYSSGGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLSSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSNQSFPPSLMSKSSSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSSFGPGEQKRYNPSSKTSNAHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQGTGNSYTDPGGPKETSSATPGRDSKTVQKGSESGRGRQKSPAQSDSTAQRRTVGKKQPKKTEKAAAEEPRGGLKIESETPVDMATSMPSSRHKAATKGSRKPNIKKESKSSPRPSAEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRANESKKPKTEDKNMAGHKPSSNKESSKQSASKEKDLLPSPAGPVPSKDPKTEHGSRKRTVSQSSSLKSSNNSNKENSASSKNSSSASKQKRTEGKTSSSSKEVKEKAPNSSSNCPPSAPTPDASKPRRTKLAFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFLMYSETVELIKYTMKLKNYLAPDATAADKRLTVLCLRCQSLLYLRLFKLKKETALKYSKTLTEHLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQRIHHMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	NM_001191129.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 4 (AFF4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191129.1

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG 
AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT 
CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC 
AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG 
AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT 
TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT 
GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT 
AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA 
GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAGTTT TGGCCCTGGA 
GAACAAAAAA GATATAATCC TTCTTCTAAA ACTTCAAATG CACACCAGTC TAAATCTATG 
TTAAAAGATG ACTTAAAGCT AAGCAGCAGT GAAGACAGTG ATGGAGAACA GGATTGTGAT 
AAGACAATGC CAAGGAGTAC ACCAGGAAGT AACTCTGAAC CTTCACACCA TAATAGTGAA 
GGGGCAGATA ACTCCAGGGA TGATTCTAGC AGCCACAGTG GATCTGAAAG CAGCTCTGGA 
TCTGACTCAG AGAGTGAAAG TAGTTCCAGT GACAGTGAGG CAAATGAGCC ATCCCAGAGT 
GCATCTCCTG AGCCTGAACC ACCTCCCACA AATAAATGGC AACTTGATAA CTGGCTGAAT 
AAAGTAAATC CACATAAAGT ATCACCAGCT TCTTCTGTGG ACAGTAATAT CCCATCATCT 
CAAGGCTATA AAAAGGAAGG CCGAGAACAG GGCACAGGGA ATAGCTACAC AGATCCAGGT 
GGTCCTAAAG AAACAAGTTC TGCTACTCCT GGACGAGACT CCAAAACTGT CCAAAAGGGA 
TCAGAAAGTG GGCGTGGGAG GCAAAAGTCT CCTGCCCAGA GTGACAGCAC AGCACAGAGG 
AGAACTGTAG GGAAAAAACA ACCCAAAAAG ACTGAGAAGG CAGCTGCTGA AGAGCCCCGT 
GGAGGCCTGA AGATAGAGAG TGAAACTCCT GTAGACATGG CTACCAGCAT GCCCTCCAGC 
AGACACAAAG CGGCCACCAA AGGCTCAAGA AAACCCAATA TAAAAAAGGA GTCCAAATCT 
TCCCCTCGAC CTTCAGCAGA GAAAAAGAAA TACAAGTCAA CAAGTAAATC TTCCCAGAAA 
TCAAGGGAAA TTATAGAAAC AGATACCTCA TCCTCAGATT CGGATGAAAG CGAGAGCCTT 
CCTCCTTCCT CACAAACTCC TAAGTACCCT GAGAGTAATA GGACTCCCGT TAAACCCTCC 
TCAGTGGAGG AAGAAGATAG CTTTTTTCGG CAACGAATGT TCTCTCCTAT GGAAGAGAAG 
GAACTTCTTT CACCCCTCAG TGAGCCTGAT GACAGGTATC CACTTATTGT GAAGATTGAC 
CTGAATCTCT TGACTAGAAT ACCAGGAAAG CCTTACAAAG AAACAGAGCC ACCCAAGGGG 
GAAAAGAAAA ATGTGCCAGA AAAGCACACA AGAGAGGCTC AGAAACAAGC CTCAGAGAAA 
GTTTCCAACA AAGGCAAGAG GAAACATAAG AATGAAGATG ATAACCGAGC CAATGAAAGC 
AAGAAACCTA AAACAGAGGA CAAGAATATG GCAGGCCACA AGCCATCCAG CAACAAAGAG 
TCATCTAAAC AGAGTGCTTC TAAAGAAAAA GATTTATTGC CTTCTCCTGC TGGGCCTGTT 
CCTTCAAAAG ATCCAAAAAC AGAGCATGGC TCTCGGAAGA GGACTGTTAG TCAGTCTTCT 
TCCTTAAAGT CAAGTAATAA CAGCAACAAG GAAAATAGTG CCAGCAGCAA AAACAGTTCC 
TCCGCATCAA AGCAGAAGAG GACTGAAGGG AAGACTTCCA GTAGCTCTAA GGAGGTCAAG 
GAAAAAGCTC CAAATAGTTC TTCTAACTGT CCTCCATCTG CACCAACTCC TGATGCTTCT 
AAGCCTCGGA GAACGAAGCT TGCCTTTGAT GACAGAAATT ATTCAGCAGA CCATTATTTA 
CAAGAAGCAA AAAAGCTAAA GCACAATGCA GATGCATTGT CTGATAGGTT TGAGAAAGCT 
GTATACTATC TTGATGCTGT GGTATCTTTC ATTGAATGTG GGAATGCATT AGAGAAAAAT 
GCTCAGGAAT CCAAATCCCC ATTCCTTATG TATTCAGAAA CGGTGGAGCT CATCAAATAC 
ACTATGAAGC TAAAGAATTA CTTGGCACCA GATGCTACAG CTGCAGATAA AAGACTTACA 
GTGCTTTGCC TGCGATGCCA GTCTTTGCTG TACCTGAGGC TCTTCAAACT GAAGAAAGAA 
ACTGCTCTGA AGTATTCAAA GACACTGACA GAGCATCTGA AGAATTCTTA CAATAACTCT 
CAAGCACCAT CACCAGGCTT GGGAAGCAAA GCTGTTGGGA TGCCTTCCCC TGTTTCTCCA 
AAGCTGTCAC CAGGCAATTC AGGAAATTAT TCTTCTGGAG CCAGTAGTGC TTCAGCAAGT 
GGTTCTTCAG TGACCATTCC ACAGAGGATT CACCACATGG CAGCCAGCTA TGTTCAGGTT 
ACATCCAACT TCCTCTATGC CACTGAAATT TGGGACCAAG CTGAACAGCT TTCCAAAGAG 
CAAAAAGAAT TCTTTGCTGA ACTGGATAAA GTAATGGGCC CTCTCATCTT TAATGCAAGC 
ATCATGACAG ACCTAGTTCG TTACACCCGG CAGGGACTGC ACTGGCTTCG CCAGGATGCC 
AAGTTGATAT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_005209304.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209361.1 Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa21434
Clone ID Related Accession (Same CDS sequence)	NM_001191129.1 , XM_005209304.4 , XM_005209304.3 , XM_024994567.1
Accession Version	XM_005209304.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209304.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG 
AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT 
CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC 
AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG 
AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT 
TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT 
GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT 
AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA 
GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAGTTT TGGCCCTGGA 
GAACAAAAAA GATATAATCC TTCTTCTAAA ACTTCAAATG CACACCAGTC TAAATCTATG 
TTAAAAGATG ACTTAAAGCT AAGCAGCAGT GAAGACAGTG ATGGAGAACA GGATTGTGAT 
AAGACAATGC CAAGGAGTAC ACCAGGAAGT AACTCTGAAC CTTCACACCA TAATAGTGAA 
GGGGCAGATA ACTCCAGGGA TGATTCTAGC AGCCACAGTG GATCTGAAAG CAGCTCTGGA 
TCTGACTCAG AGAGTGAAAG TAGTTCCAGT GACAGTGAGG CAAATGAGCC ATCCCAGAGT 
GCATCTCCTG AGCCTGAACC ACCTCCCACA AATAAATGGC AACTTGATAA CTGGCTGAAT 
AAAGTAAATC CACATAAAGT ATCACCAGCT TCTTCTGTGG ACAGTAATAT CCCATCATCT 
CAAGGCTATA AAAAGGAAGG CCGAGAACAG GGCACAGGGA ATAGCTACAC AGATCCAGGT 
GGTCCTAAAG AAACAAGTTC TGCTACTCCT GGACGAGACT CCAAAACTGT CCAAAAGGGA 
TCAGAAAGTG GGCGTGGGAG GCAAAAGTCT CCTGCCCAGA GTGACAGCAC AGCACAGAGG 
AGAACTGTAG GGAAAAAACA ACCCAAAAAG ACTGAGAAGG CAGCTGCTGA AGAGCCCCGT 
GGAGGCCTGA AGATAGAGAG TGAAACTCCT GTAGACATGG CTACCAGCAT GCCCTCCAGC 
AGACACAAAG CGGCCACCAA AGGCTCAAGA AAACCCAATA TAAAAAAGGA GTCCAAATCT 
TCCCCTCGAC CTTCAGCAGA GAAAAAGAAA TACAAGTCAA CAAGTAAATC TTCCCAGAAA 
TCAAGGGAAA TTATAGAAAC AGATACCTCA TCCTCAGATT CGGATGAAAG CGAGAGCCTT 
CCTCCTTCCT CACAAACTCC TAAGTACCCT GAGAGTAATA GGACTCCCGT TAAACCCTCC 
TCAGTGGAGG AAGAAGATAG CTTTTTTCGG CAACGAATGT TCTCTCCTAT GGAAGAGAAG 
GAACTTCTTT CACCCCTCAG TGAGCCTGAT GACAGGTATC CACTTATTGT GAAGATTGAC 
CTGAATCTCT TGACTAGAAT ACCAGGAAAG CCTTACAAAG AAACAGAGCC ACCCAAGGGG 
GAAAAGAAAA ATGTGCCAGA AAAGCACACA AGAGAGGCTC AGAAACAAGC CTCAGAGAAA 
GTTTCCAACA AAGGCAAGAG GAAACATAAG AATGAAGATG ATAACCGAGC CAATGAAAGC 
AAGAAACCTA AAACAGAGGA CAAGAATATG GCAGGCCACA AGCCATCCAG CAACAAAGAG 
TCATCTAAAC AGAGTGCTTC TAAAGAAAAA GATTTATTGC CTTCTCCTGC TGGGCCTGTT 
CCTTCAAAAG ATCCAAAAAC AGAGCATGGC TCTCGGAAGA GGACTGTTAG TCAGTCTTCT 
TCCTTAAAGT CAAGTAATAA CAGCAACAAG GAAAATAGTG CCAGCAGCAA AAACAGTTCC 
TCCGCATCAA AGCAGAAGAG GACTGAAGGG AAGACTTCCA GTAGCTCTAA GGAGGTCAAG 
GAAAAAGCTC CAAATAGTTC TTCTAACTGT CCTCCATCTG CACCAACTCC TGATGCTTCT 
AAGCCTCGGA GAACGAAGCT TGCCTTTGAT GACAGAAATT ATTCAGCAGA CCATTATTTA 
CAAGAAGCAA AAAAGCTAAA GCACAATGCA GATGCATTGT CTGATAGGTT TGAGAAAGCT 
GTATACTATC TTGATGCTGT GGTATCTTTC ATTGAATGTG GGAATGCATT AGAGAAAAAT 
GCTCAGGAAT CCAAATCCCC ATTCCTTATG TATTCAGAAA CGGTGGAGCT CATCAAATAC 
ACTATGAAGC TAAAGAATTA CTTGGCACCA GATGCTACAG CTGCAGATAA AAGACTTACA 
GTGCTTTGCC TGCGATGCCA GTCTTTGCTG TACCTGAGGC TCTTCAAACT GAAGAAAGAA 
ACTGCTCTGA AGTATTCAAA GACACTGACA GAGCATCTGA AGAATTCTTA CAATAACTCT 
CAAGCACCAT CACCAGGCTT GGGAAGCAAA GCTGTTGGGA TGCCTTCCCC TGTTTCTCCA 
AAGCTGTCAC CAGGCAATTC AGGAAATTAT TCTTCTGGAG CCAGTAGTGC TTCAGCAAGT 
GGTTCTTCAG TGACCATTCC ACAGAGGATT CACCACATGG CAGCCAGCTA TGTTCAGGTT 
ACATCCAACT TCCTCTATGC CACTGAAATT TGGGACCAAG CTGAACAGCT TTCCAAAGAG 
CAAAAAGAAT TCTTTGCTGA ACTGGATAAA GTAATGGGCC CTCTCATCTT TAATGCAAGC 
ATCATGACAG ACCTAGTTCG TTACACCCGG CAGGGACTGC ACTGGCTTCG CCAGGATGCC 
AAGTTGATAT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209361.1
CDS	365..3859
Translation	MNREDRNVLRMKERERRNQEIQQGDDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPTADEKSNPNFFEQRHGSSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRASAGASGGTNSSGQRHDRDSYSSGGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLSSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSNQSFPPSLMSKSSSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSSFGPGEQKRYNPSSKTSNAHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQGTGNSYTDPGGPKETSSATPGRDSKTVQKGSESGRGRQKSPAQSDSTAQRRTVGKKQPKKTEKAAAEEPRGGLKIESETPVDMATSMPSSRHKAATKGSRKPNIKKESKSSPRPSAEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRANESKKPKTEDKNMAGHKPSSNKESSKQSASKEKDLLPSPAGPVPSKDPKTEHGSRKRTVSQSSSLKSSNNSNKENSASSKNSSSASKQKRTEGKTSSSSKEVKEKAPNSSSNCPPSAPTPDASKPRRTKLAFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFLMYSETVELIKYTMKLKNYLAPDATAADKRLTVLCLRCQSLLYLRLFKLKKETALKYSKTLTEHLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQRIHHMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	XM_005209304.4
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209304.4

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG 
AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT 
CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC 
AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG 
AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT 
TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT 
GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT 
AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA 
GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAGTTT TGGCCCTGGA 
GAACAAAAAA GATATAATCC TTCTTCTAAA ACTTCAAATG CACACCAGTC TAAATCTATG 
TTAAAAGATG ACTTAAAGCT AAGCAGCAGT GAAGACAGTG ATGGAGAACA GGATTGTGAT 
AAGACAATGC CAAGGAGTAC ACCAGGAAGT AACTCTGAAC CTTCACACCA TAATAGTGAA 
GGGGCAGATA ACTCCAGGGA TGATTCTAGC AGCCACAGTG GATCTGAAAG CAGCTCTGGA 
TCTGACTCAG AGAGTGAAAG TAGTTCCAGT GACAGTGAGG CAAATGAGCC ATCCCAGAGT 
GCATCTCCTG AGCCTGAACC ACCTCCCACA AATAAATGGC AACTTGATAA CTGGCTGAAT 
AAAGTAAATC CACATAAAGT ATCACCAGCT TCTTCTGTGG ACAGTAATAT CCCATCATCT 
CAAGGCTATA AAAAGGAAGG CCGAGAACAG GGCACAGGGA ATAGCTACAC AGATCCAGGT 
GGTCCTAAAG AAACAAGTTC TGCTACTCCT GGACGAGACT CCAAAACTGT CCAAAAGGGA 
TCAGAAAGTG GGCGTGGGAG GCAAAAGTCT CCTGCCCAGA GTGACAGCAC AGCACAGAGG 
AGAACTGTAG GGAAAAAACA ACCCAAAAAG ACTGAGAAGG CAGCTGCTGA AGAGCCCCGT 
GGAGGCCTGA AGATAGAGAG TGAAACTCCT GTAGACATGG CTACCAGCAT GCCCTCCAGC 
AGACACAAAG CGGCCACCAA AGGCTCAAGA AAACCCAATA TAAAAAAGGA GTCCAAATCT 
TCCCCTCGAC CTTCAGCAGA GAAAAAGAAA TACAAGTCAA CAAGTAAATC TTCCCAGAAA 
TCAAGGGAAA TTATAGAAAC AGATACCTCA TCCTCAGATT CGGATGAAAG CGAGAGCCTT 
CCTCCTTCCT CACAAACTCC TAAGTACCCT GAGAGTAATA GGACTCCCGT TAAACCCTCC 
TCAGTGGAGG AAGAAGATAG CTTTTTTCGG CAACGAATGT TCTCTCCTAT GGAAGAGAAG 
GAACTTCTTT CACCCCTCAG TGAGCCTGAT GACAGGTATC CACTTATTGT GAAGATTGAC 
CTGAATCTCT TGACTAGAAT ACCAGGAAAG CCTTACAAAG AAACAGAGCC ACCCAAGGGG 
GAAAAGAAAA ATGTGCCAGA AAAGCACACA AGAGAGGCTC AGAAACAAGC CTCAGAGAAA 
GTTTCCAACA AAGGCAAGAG GAAACATAAG AATGAAGATG ATAACCGAGC CAATGAAAGC 
AAGAAACCTA AAACAGAGGA CAAGAATATG GCAGGCCACA AGCCATCCAG CAACAAAGAG 
TCATCTAAAC AGAGTGCTTC TAAAGAAAAA GATTTATTGC CTTCTCCTGC TGGGCCTGTT 
CCTTCAAAAG ATCCAAAAAC AGAGCATGGC TCTCGGAAGA GGACTGTTAG TCAGTCTTCT 
TCCTTAAAGT CAAGTAATAA CAGCAACAAG GAAAATAGTG CCAGCAGCAA AAACAGTTCC 
TCCGCATCAA AGCAGAAGAG GACTGAAGGG AAGACTTCCA GTAGCTCTAA GGAGGTCAAG 
GAAAAAGCTC CAAATAGTTC TTCTAACTGT CCTCCATCTG CACCAACTCC TGATGCTTCT 
AAGCCTCGGA GAACGAAGCT TGCCTTTGAT GACAGAAATT ATTCAGCAGA CCATTATTTA 
CAAGAAGCAA AAAAGCTAAA GCACAATGCA GATGCATTGT CTGATAGGTT TGAGAAAGCT 
GTATACTATC TTGATGCTGT GGTATCTTTC ATTGAATGTG GGAATGCATT AGAGAAAAAT 
GCTCAGGAAT CCAAATCCCC ATTCCTTATG TATTCAGAAA CGGTGGAGCT CATCAAATAC 
ACTATGAAGC TAAAGAATTA CTTGGCACCA GATGCTACAG CTGCAGATAA AAGACTTACA 
GTGCTTTGCC TGCGATGCCA GTCTTTGCTG TACCTGAGGC TCTTCAAACT GAAGAAAGAA 
ACTGCTCTGA AGTATTCAAA GACACTGACA GAGCATCTGA AGAATTCTTA CAATAACTCT 
CAAGCACCAT CACCAGGCTT GGGAAGCAAA GCTGTTGGGA TGCCTTCCCC TGTTTCTCCA 
AAGCTGTCAC CAGGCAATTC AGGAAATTAT TCTTCTGGAG CCAGTAGTGC TTCAGCAAGT 
GGTTCTTCAG TGACCATTCC ACAGAGGATT CACCACATGG CAGCCAGCTA TGTTCAGGTT 
ACATCCAACT TCCTCTATGC CACTGAAATT TGGGACCAAG CTGAACAGCT TTCCAAAGAG 
CAAAAAGAAT TCTTTGCTGA ACTGGATAAA GTAATGGGCC CTCTCATCTT TAATGCAAGC 
ATCATGACAG ACCTAGTTCG TTACACCCGG CAGGGACTGC ACTGGCTTCG CCAGGATGCC 
AAGTTGATAT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_005209304.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209361.1 Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.

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CloneID	OBa21434
Clone ID Related Accession (Same CDS sequence)	NM_001191129.1 , XM_005209304.4 , XM_005209304.3 , XM_024994567.1
Accession Version	XM_005209304.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209304.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG 
AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT 
CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC 
AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG 
AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT 
TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT 
GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT 
AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA 
GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAGTTT TGGCCCTGGA 
GAACAAAAAA GATATAATCC TTCTTCTAAA ACTTCAAATG CACACCAGTC TAAATCTATG 
TTAAAAGATG ACTTAAAGCT AAGCAGCAGT GAAGACAGTG ATGGAGAACA GGATTGTGAT 
AAGACAATGC CAAGGAGTAC ACCAGGAAGT AACTCTGAAC CTTCACACCA TAATAGTGAA 
GGGGCAGATA ACTCCAGGGA TGATTCTAGC AGCCACAGTG GATCTGAAAG CAGCTCTGGA 
TCTGACTCAG AGAGTGAAAG TAGTTCCAGT GACAGTGAGG CAAATGAGCC ATCCCAGAGT 
GCATCTCCTG AGCCTGAACC ACCTCCCACA AATAAATGGC AACTTGATAA CTGGCTGAAT 
AAAGTAAATC CACATAAAGT ATCACCAGCT TCTTCTGTGG ACAGTAATAT CCCATCATCT 
CAAGGCTATA AAAAGGAAGG CCGAGAACAG GGCACAGGGA ATAGCTACAC AGATCCAGGT 
GGTCCTAAAG AAACAAGTTC TGCTACTCCT GGACGAGACT CCAAAACTGT CCAAAAGGGA 
TCAGAAAGTG GGCGTGGGAG GCAAAAGTCT CCTGCCCAGA GTGACAGCAC AGCACAGAGG 
AGAACTGTAG GGAAAAAACA ACCCAAAAAG ACTGAGAAGG CAGCTGCTGA AGAGCCCCGT 
GGAGGCCTGA AGATAGAGAG TGAAACTCCT GTAGACATGG CTACCAGCAT GCCCTCCAGC 
AGACACAAAG CGGCCACCAA AGGCTCAAGA AAACCCAATA TAAAAAAGGA GTCCAAATCT 
TCCCCTCGAC CTTCAGCAGA GAAAAAGAAA TACAAGTCAA CAAGTAAATC TTCCCAGAAA 
TCAAGGGAAA TTATAGAAAC AGATACCTCA TCCTCAGATT CGGATGAAAG CGAGAGCCTT 
CCTCCTTCCT CACAAACTCC TAAGTACCCT GAGAGTAATA GGACTCCCGT TAAACCCTCC 
TCAGTGGAGG AAGAAGATAG CTTTTTTCGG CAACGAATGT TCTCTCCTAT GGAAGAGAAG 
GAACTTCTTT CACCCCTCAG TGAGCCTGAT GACAGGTATC CACTTATTGT GAAGATTGAC 
CTGAATCTCT TGACTAGAAT ACCAGGAAAG CCTTACAAAG AAACAGAGCC ACCCAAGGGG 
GAAAAGAAAA ATGTGCCAGA AAAGCACACA AGAGAGGCTC AGAAACAAGC CTCAGAGAAA 
GTTTCCAACA AAGGCAAGAG GAAACATAAG AATGAAGATG ATAACCGAGC CAATGAAAGC 
AAGAAACCTA AAACAGAGGA CAAGAATATG GCAGGCCACA AGCCATCCAG CAACAAAGAG 
TCATCTAAAC AGAGTGCTTC TAAAGAAAAA GATTTATTGC CTTCTCCTGC TGGGCCTGTT 
CCTTCAAAAG ATCCAAAAAC AGAGCATGGC TCTCGGAAGA GGACTGTTAG TCAGTCTTCT 
TCCTTAAAGT CAAGTAATAA CAGCAACAAG GAAAATAGTG CCAGCAGCAA AAACAGTTCC 
TCCGCATCAA AGCAGAAGAG GACTGAAGGG AAGACTTCCA GTAGCTCTAA GGAGGTCAAG 
GAAAAAGCTC CAAATAGTTC TTCTAACTGT CCTCCATCTG CACCAACTCC TGATGCTTCT 
AAGCCTCGGA GAACGAAGCT TGCCTTTGAT GACAGAAATT ATTCAGCAGA CCATTATTTA 
CAAGAAGCAA AAAAGCTAAA GCACAATGCA GATGCATTGT CTGATAGGTT TGAGAAAGCT 
GTATACTATC TTGATGCTGT GGTATCTTTC ATTGAATGTG GGAATGCATT AGAGAAAAAT 
GCTCAGGAAT CCAAATCCCC ATTCCTTATG TATTCAGAAA CGGTGGAGCT CATCAAATAC 
ACTATGAAGC TAAAGAATTA CTTGGCACCA GATGCTACAG CTGCAGATAA AAGACTTACA 
GTGCTTTGCC TGCGATGCCA GTCTTTGCTG TACCTGAGGC TCTTCAAACT GAAGAAAGAA 
ACTGCTCTGA AGTATTCAAA GACACTGACA GAGCATCTGA AGAATTCTTA CAATAACTCT 
CAAGCACCAT CACCAGGCTT GGGAAGCAAA GCTGTTGGGA TGCCTTCCCC TGTTTCTCCA 
AAGCTGTCAC CAGGCAATTC AGGAAATTAT TCTTCTGGAG CCAGTAGTGC TTCAGCAAGT 
GGTTCTTCAG TGACCATTCC ACAGAGGATT CACCACATGG CAGCCAGCTA TGTTCAGGTT 
ACATCCAACT TCCTCTATGC CACTGAAATT TGGGACCAAG CTGAACAGCT TTCCAAAGAG 
CAAAAAGAAT TCTTTGCTGA ACTGGATAAA GTAATGGGCC CTCTCATCTT TAATGCAAGC 
ATCATGACAG ACCTAGTTCG TTACACCCGG CAGGGACTGC ACTGGCTTCG CCAGGATGCC 
AAGTTGATAT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209361.1
CDS	367..3861
Translation	MNREDRNVLRMKERERRNQEIQQGDDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPTADEKSNPNFFEQRHGSSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRASAGASGGTNSSGQRHDRDSYSSGGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLSSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSNQSFPPSLMSKSSSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSSFGPGEQKRYNPSSKTSNAHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQGTGNSYTDPGGPKETSSATPGRDSKTVQKGSESGRGRQKSPAQSDSTAQRRTVGKKQPKKTEKAAAEEPRGGLKIESETPVDMATSMPSSRHKAATKGSRKPNIKKESKSSPRPSAEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRANESKKPKTEDKNMAGHKPSSNKESSKQSASKEKDLLPSPAGPVPSKDPKTEHGSRKRTVSQSSSLKSSNNSNKENSASSKNSSSASKQKRTEGKTSSSSKEVKEKAPNSSSNCPPSAPTPDASKPRRTKLAFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFLMYSETVELIKYTMKLKNYLAPDATAADKRLTVLCLRCQSLLYLRLFKLKKETALKYSKTLTEHLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQRIHHMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	XM_005209304.3
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209304.3

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG 
AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT 
CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC 
AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG 
AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT 
TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT 
GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT 
AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA 
GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAGTTT TGGCCCTGGA 
GAACAAAAAA GATATAATCC TTCTTCTAAA ACTTCAAATG CACACCAGTC TAAATCTATG 
TTAAAAGATG ACTTAAAGCT AAGCAGCAGT GAAGACAGTG ATGGAGAACA GGATTGTGAT 
AAGACAATGC CAAGGAGTAC ACCAGGAAGT AACTCTGAAC CTTCACACCA TAATAGTGAA 
GGGGCAGATA ACTCCAGGGA TGATTCTAGC AGCCACAGTG GATCTGAAAG CAGCTCTGGA 
TCTGACTCAG AGAGTGAAAG TAGTTCCAGT GACAGTGAGG CAAATGAGCC ATCCCAGAGT 
GCATCTCCTG AGCCTGAACC ACCTCCCACA AATAAATGGC AACTTGATAA CTGGCTGAAT 
AAAGTAAATC CACATAAAGT ATCACCAGCT TCTTCTGTGG ACAGTAATAT CCCATCATCT 
CAAGGCTATA AAAAGGAAGG CCGAGAACAG GGCACAGGGA ATAGCTACAC AGATCCAGGT 
GGTCCTAAAG AAACAAGTTC TGCTACTCCT GGACGAGACT CCAAAACTGT CCAAAAGGGA 
TCAGAAAGTG GGCGTGGGAG GCAAAAGTCT CCTGCCCAGA GTGACAGCAC AGCACAGAGG 
AGAACTGTAG GGAAAAAACA ACCCAAAAAG ACTGAGAAGG CAGCTGCTGA AGAGCCCCGT 
GGAGGCCTGA AGATAGAGAG TGAAACTCCT GTAGACATGG CTACCAGCAT GCCCTCCAGC 
AGACACAAAG CGGCCACCAA AGGCTCAAGA AAACCCAATA TAAAAAAGGA GTCCAAATCT 
TCCCCTCGAC CTTCAGCAGA GAAAAAGAAA TACAAGTCAA CAAGTAAATC TTCCCAGAAA 
TCAAGGGAAA TTATAGAAAC AGATACCTCA TCCTCAGATT CGGATGAAAG CGAGAGCCTT 
CCTCCTTCCT CACAAACTCC TAAGTACCCT GAGAGTAATA GGACTCCCGT TAAACCCTCC 
TCAGTGGAGG AAGAAGATAG CTTTTTTCGG CAACGAATGT TCTCTCCTAT GGAAGAGAAG 
GAACTTCTTT CACCCCTCAG TGAGCCTGAT GACAGGTATC CACTTATTGT GAAGATTGAC 
CTGAATCTCT TGACTAGAAT ACCAGGAAAG CCTTACAAAG AAACAGAGCC ACCCAAGGGG 
GAAAAGAAAA ATGTGCCAGA AAAGCACACA AGAGAGGCTC AGAAACAAGC CTCAGAGAAA 
GTTTCCAACA AAGGCAAGAG GAAACATAAG AATGAAGATG ATAACCGAGC CAATGAAAGC 
AAGAAACCTA AAACAGAGGA CAAGAATATG GCAGGCCACA AGCCATCCAG CAACAAAGAG 
TCATCTAAAC AGAGTGCTTC TAAAGAAAAA GATTTATTGC CTTCTCCTGC TGGGCCTGTT 
CCTTCAAAAG ATCCAAAAAC AGAGCATGGC TCTCGGAAGA GGACTGTTAG TCAGTCTTCT 
TCCTTAAAGT CAAGTAATAA CAGCAACAAG GAAAATAGTG CCAGCAGCAA AAACAGTTCC 
TCCGCATCAA AGCAGAAGAG GACTGAAGGG AAGACTTCCA GTAGCTCTAA GGAGGTCAAG 
GAAAAAGCTC CAAATAGTTC TTCTAACTGT CCTCCATCTG CACCAACTCC TGATGCTTCT 
AAGCCTCGGA GAACGAAGCT TGCCTTTGAT GACAGAAATT ATTCAGCAGA CCATTATTTA 
CAAGAAGCAA AAAAGCTAAA GCACAATGCA GATGCATTGT CTGATAGGTT TGAGAAAGCT 
GTATACTATC TTGATGCTGT GGTATCTTTC ATTGAATGTG GGAATGCATT AGAGAAAAAT 
GCTCAGGAAT CCAAATCCCC ATTCCTTATG TATTCAGAAA CGGTGGAGCT CATCAAATAC 
ACTATGAAGC TAAAGAATTA CTTGGCACCA GATGCTACAG CTGCAGATAA AAGACTTACA 
GTGCTTTGCC TGCGATGCCA GTCTTTGCTG TACCTGAGGC TCTTCAAACT GAAGAAAGAA 
ACTGCTCTGA AGTATTCAAA GACACTGACA GAGCATCTGA AGAATTCTTA CAATAACTCT 
CAAGCACCAT CACCAGGCTT GGGAAGCAAA GCTGTTGGGA TGCCTTCCCC TGTTTCTCCA 
AAGCTGTCAC CAGGCAATTC AGGAAATTAT TCTTCTGGAG CCAGTAGTGC TTCAGCAAGT 
GGTTCTTCAG TGACCATTCC ACAGAGGATT CACCACATGG CAGCCAGCTA TGTTCAGGTT 
ACATCCAACT TCCTCTATGC CACTGAAATT TGGGACCAAG CTGAACAGCT TTCCAAAGAG 
CAAAAAGAAT TCTTTGCTGA ACTGGATAAA GTAATGGGCC CTCTCATCTT TAATGCAAGC 
ATCATGACAG ACCTAGTTCG TTACACCCGG CAGGGACTGC ACTGGCTTCG CCAGGATGCC 
AAGTTGATAT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_024994567.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850335.1 Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.

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CloneID	OBa21434
Clone ID Related Accession (Same CDS sequence)	NM_001191129.1 , XM_005209304.4 , XM_005209304.3 , XM_024994567.1
Accession Version	XM_024994567.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG 
AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT 
CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC 
AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG 
AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT 
TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT 
GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT 
AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA 
GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAGTTT TGGCCCTGGA 
GAACAAAAAA GATATAATCC TTCTTCTAAA ACTTCAAATG CACACCAGTC TAAATCTATG 
TTAAAAGATG ACTTAAAGCT AAGCAGCAGT GAAGACAGTG ATGGAGAACA GGATTGTGAT 
AAGACAATGC CAAGGAGTAC ACCAGGAAGT AACTCTGAAC CTTCACACCA TAATAGTGAA 
GGGGCAGATA ACTCCAGGGA TGATTCTAGC AGCCACAGTG GATCTGAAAG CAGCTCTGGA 
TCTGACTCAG AGAGTGAAAG TAGTTCCAGT GACAGTGAGG CAAATGAGCC ATCCCAGAGT 
GCATCTCCTG AGCCTGAACC ACCTCCCACA AATAAATGGC AACTTGATAA CTGGCTGAAT 
AAAGTAAATC CACATAAAGT ATCACCAGCT TCTTCTGTGG ACAGTAATAT CCCATCATCT 
CAAGGCTATA AAAAGGAAGG CCGAGAACAG GGCACAGGGA ATAGCTACAC AGATCCAGGT 
GGTCCTAAAG AAACAAGTTC TGCTACTCCT GGACGAGACT CCAAAACTGT CCAAAAGGGA 
TCAGAAAGTG GGCGTGGGAG GCAAAAGTCT CCTGCCCAGA GTGACAGCAC AGCACAGAGG 
AGAACTGTAG GGAAAAAACA ACCCAAAAAG ACTGAGAAGG CAGCTGCTGA AGAGCCCCGT 
GGAGGCCTGA AGATAGAGAG TGAAACTCCT GTAGACATGG CTACCAGCAT GCCCTCCAGC 
AGACACAAAG CGGCCACCAA AGGCTCAAGA AAACCCAATA TAAAAAAGGA GTCCAAATCT 
TCCCCTCGAC CTTCAGCAGA GAAAAAGAAA TACAAGTCAA CAAGTAAATC TTCCCAGAAA 
TCAAGGGAAA TTATAGAAAC AGATACCTCA TCCTCAGATT CGGATGAAAG CGAGAGCCTT 
CCTCCTTCCT CACAAACTCC TAAGTACCCT GAGAGTAATA GGACTCCCGT TAAACCCTCC 
TCAGTGGAGG AAGAAGATAG CTTTTTTCGG CAACGAATGT TCTCTCCTAT GGAAGAGAAG 
GAACTTCTTT CACCCCTCAG TGAGCCTGAT GACAGGTATC CACTTATTGT GAAGATTGAC 
CTGAATCTCT TGACTAGAAT ACCAGGAAAG CCTTACAAAG AAACAGAGCC ACCCAAGGGG 
GAAAAGAAAA ATGTGCCAGA AAAGCACACA AGAGAGGCTC AGAAACAAGC CTCAGAGAAA 
GTTTCCAACA AAGGCAAGAG GAAACATAAG AATGAAGATG ATAACCGAGC CAATGAAAGC 
AAGAAACCTA AAACAGAGGA CAAGAATATG GCAGGCCACA AGCCATCCAG CAACAAAGAG 
TCATCTAAAC AGAGTGCTTC TAAAGAAAAA GATTTATTGC CTTCTCCTGC TGGGCCTGTT 
CCTTCAAAAG ATCCAAAAAC AGAGCATGGC TCTCGGAAGA GGACTGTTAG TCAGTCTTCT 
TCCTTAAAGT CAAGTAATAA CAGCAACAAG GAAAATAGTG CCAGCAGCAA AAACAGTTCC 
TCCGCATCAA AGCAGAAGAG GACTGAAGGG AAGACTTCCA GTAGCTCTAA GGAGGTCAAG 
GAAAAAGCTC CAAATAGTTC TTCTAACTGT CCTCCATCTG CACCAACTCC TGATGCTTCT 
AAGCCTCGGA GAACGAAGCT TGCCTTTGAT GACAGAAATT ATTCAGCAGA CCATTATTTA 
CAAGAAGCAA AAAAGCTAAA GCACAATGCA GATGCATTGT CTGATAGGTT TGAGAAAGCT 
GTATACTATC TTGATGCTGT GGTATCTTTC ATTGAATGTG GGAATGCATT AGAGAAAAAT 
GCTCAGGAAT CCAAATCCCC ATTCCTTATG TATTCAGAAA CGGTGGAGCT CATCAAATAC 
ACTATGAAGC TAAAGAATTA CTTGGCACCA GATGCTACAG CTGCAGATAA AAGACTTACA 
GTGCTTTGCC TGCGATGCCA GTCTTTGCTG TACCTGAGGC TCTTCAAACT GAAGAAAGAA 
ACTGCTCTGA AGTATTCAAA GACACTGACA GAGCATCTGA AGAATTCTTA CAATAACTCT 
CAAGCACCAT CACCAGGCTT GGGAAGCAAA GCTGTTGGGA TGCCTTCCCC TGTTTCTCCA 
AAGCTGTCAC CAGGCAATTC AGGAAATTAT TCTTCTGGAG CCAGTAGTGC TTCAGCAAGT 
GGTTCTTCAG TGACCATTCC ACAGAGGATT CACCACATGG CAGCCAGCTA TGTTCAGGTT 
ACATCCAACT TCCTCTATGC CACTGAAATT TGGGACCAAG CTGAACAGCT TTCCAAAGAG 
CAAAAAGAAT TCTTTGCTGA ACTGGATAAA GTAATGGGCC CTCTCATCTT TAATGCAAGC 
ATCATGACAG ACCTAGTTCG TTACACCCGG CAGGGACTGC ACTGGCTTCG CCAGGATGCC 
AAGTTGATAT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850335.1
CDS	24..3518
Translation	MNREDRNVLRMKERERRNQEIQQGDDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPTADEKSNPNFFEQRHGSSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRASAGASGGTNSSGQRHDRDSYSSGGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLSSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSNQSFPPSLMSKSSSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSSFGPGEQKRYNPSSKTSNAHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQGTGNSYTDPGGPKETSSATPGRDSKTVQKGSESGRGRQKSPAQSDSTAQRRTVGKKQPKKTEKAAAEEPRGGLKIESETPVDMATSMPSSRHKAATKGSRKPNIKKESKSSPRPSAEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRANESKKPKTEDKNMAGHKPSSNKESSKQSASKEKDLLPSPAGPVPSKDPKTEHGSRKRTVSQSSSLKSSNNSNKENSASSKNSSSASKQKRTEGKTSSSSKEVKEKAPNSSSNCPPSAPTPDASKPRRTKLAFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFLMYSETVELIKYTMKLKNYLAPDATAADKRLTVLCLRCQSLLYLRLFKLKKETALKYSKTLTEHLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQRIHHMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	XM_024994567.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024994567.1

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG 
AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT 
CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC 
AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG 
AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT 
TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT 
GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT 
AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA 
GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAGTTT TGGCCCTGGA 
GAACAAAAAA GATATAATCC TTCTTCTAAA ACTTCAAATG CACACCAGTC TAAATCTATG 
TTAAAAGATG ACTTAAAGCT AAGCAGCAGT GAAGACAGTG ATGGAGAACA GGATTGTGAT 
AAGACAATGC CAAGGAGTAC ACCAGGAAGT AACTCTGAAC CTTCACACCA TAATAGTGAA 
GGGGCAGATA ACTCCAGGGA TGATTCTAGC AGCCACAGTG GATCTGAAAG CAGCTCTGGA 
TCTGACTCAG AGAGTGAAAG TAGTTCCAGT GACAGTGAGG CAAATGAGCC ATCCCAGAGT 
GCATCTCCTG AGCCTGAACC ACCTCCCACA AATAAATGGC AACTTGATAA CTGGCTGAAT 
AAAGTAAATC CACATAAAGT ATCACCAGCT TCTTCTGTGG ACAGTAATAT CCCATCATCT 
CAAGGCTATA AAAAGGAAGG CCGAGAACAG GGCACAGGGA ATAGCTACAC AGATCCAGGT 
GGTCCTAAAG AAACAAGTTC TGCTACTCCT GGACGAGACT CCAAAACTGT CCAAAAGGGA 
TCAGAAAGTG GGCGTGGGAG GCAAAAGTCT CCTGCCCAGA GTGACAGCAC AGCACAGAGG 
AGAACTGTAG GGAAAAAACA ACCCAAAAAG ACTGAGAAGG CAGCTGCTGA AGAGCCCCGT 
GGAGGCCTGA AGATAGAGAG TGAAACTCCT GTAGACATGG CTACCAGCAT GCCCTCCAGC 
AGACACAAAG CGGCCACCAA AGGCTCAAGA AAACCCAATA TAAAAAAGGA GTCCAAATCT 
TCCCCTCGAC CTTCAGCAGA GAAAAAGAAA TACAAGTCAA CAAGTAAATC TTCCCAGAAA 
TCAAGGGAAA TTATAGAAAC AGATACCTCA TCCTCAGATT CGGATGAAAG CGAGAGCCTT 
CCTCCTTCCT CACAAACTCC TAAGTACCCT GAGAGTAATA GGACTCCCGT TAAACCCTCC 
TCAGTGGAGG AAGAAGATAG CTTTTTTCGG CAACGAATGT TCTCTCCTAT GGAAGAGAAG 
GAACTTCTTT CACCCCTCAG TGAGCCTGAT GACAGGTATC CACTTATTGT GAAGATTGAC 
CTGAATCTCT TGACTAGAAT ACCAGGAAAG CCTTACAAAG AAACAGAGCC ACCCAAGGGG 
GAAAAGAAAA ATGTGCCAGA AAAGCACACA AGAGAGGCTC AGAAACAAGC CTCAGAGAAA 
GTTTCCAACA AAGGCAAGAG GAAACATAAG AATGAAGATG ATAACCGAGC CAATGAAAGC 
AAGAAACCTA AAACAGAGGA CAAGAATATG GCAGGCCACA AGCCATCCAG CAACAAAGAG 
TCATCTAAAC AGAGTGCTTC TAAAGAAAAA GATTTATTGC CTTCTCCTGC TGGGCCTGTT 
CCTTCAAAAG ATCCAAAAAC AGAGCATGGC TCTCGGAAGA GGACTGTTAG TCAGTCTTCT 
TCCTTAAAGT CAAGTAATAA CAGCAACAAG GAAAATAGTG CCAGCAGCAA AAACAGTTCC 
TCCGCATCAA AGCAGAAGAG GACTGAAGGG AAGACTTCCA GTAGCTCTAA GGAGGTCAAG 
GAAAAAGCTC CAAATAGTTC TTCTAACTGT CCTCCATCTG CACCAACTCC TGATGCTTCT 
AAGCCTCGGA GAACGAAGCT TGCCTTTGAT GACAGAAATT ATTCAGCAGA CCATTATTTA 
CAAGAAGCAA AAAAGCTAAA GCACAATGCA GATGCATTGT CTGATAGGTT TGAGAAAGCT 
GTATACTATC TTGATGCTGT GGTATCTTTC ATTGAATGTG GGAATGCATT AGAGAAAAAT 
GCTCAGGAAT CCAAATCCCC ATTCCTTATG TATTCAGAAA CGGTGGAGCT CATCAAATAC 
ACTATGAAGC TAAAGAATTA CTTGGCACCA GATGCTACAG CTGCAGATAA AAGACTTACA 
GTGCTTTGCC TGCGATGCCA GTCTTTGCTG TACCTGAGGC TCTTCAAACT GAAGAAAGAA 
ACTGCTCTGA AGTATTCAAA GACACTGACA GAGCATCTGA AGAATTCTTA CAATAACTCT 
CAAGCACCAT CACCAGGCTT GGGAAGCAAA GCTGTTGGGA TGCCTTCCCC TGTTTCTCCA 
AAGCTGTCAC CAGGCAATTC AGGAAATTAT TCTTCTGGAG CCAGTAGTGC TTCAGCAAGT 
GGTTCTTCAG TGACCATTCC ACAGAGGATT CACCACATGG CAGCCAGCTA TGTTCAGGTT 
ACATCCAACT TCCTCTATGC CACTGAAATT TGGGACCAAG CTGAACAGCT TTCCAAAGAG 
CAAAAAGAAT TCTTTGCTGA ACTGGATAAA GTAATGGGCC CTCTCATCTT TAATGCAAGC 
ATCATGACAG ACCTAGTTCG TTACACCCGG CAGGGACTGC ACTGGCTTCG CCAGGATGCC 
AAGTTGATAT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_010807254.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805556.1 Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.

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CloneID	OBa21435
Clone ID Related Accession (Same CDS sequence)	XM_010807254.2 , XM_010807254.3
Accession Version	XM_010807254.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807254.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG 
AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT 
CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC 
AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG 
AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT 
TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT 
GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT 
AAAGAGTCTC AGCAGTCCAG TTTTGGCCCT GGAGAACAAA AAAGATATAA TCCTTCTTCT 
AAAACTTCAA ATGCACACCA GTCTAAATCT ATGTTAAAAG ATGACTTAAA GCTAAGCAGC 
AGTGAAGACA GTGATGGAGA ACAGGATTGT GATAAGACAA TGCCAAGGAG TACACCAGGA 
AGTAACTCTG AACCTTCACA CCATAATAGT GAAGGGGCAG ATAACTCCAG GGATGATTCT 
AGCAGCCACA GTGGATCTGA AAGCAGCTCT GGATCTGACT CAGAGAGTGA AAGTAGTTCC 
AGTGACAGTG AGGCAAATGA GCCATCCCAG AGTGCATCTC CTGAGCCTGA ACCACCTCCC 
ACAAATAAAT GGCAACTTGA TAACTGGCTG AATAAAGTAA ATCCACATAA AGTATCACCA 
GCTTCTTCTG TGGACAGTAA TATCCCATCA TCTCAAGGCT ATAAAAAGGA AGGCCGAGAA 
CAGGGCACAG GGAATAGCTA CACAGATCCA GGTGGTCCTA AAGAAACAAG TTCTGCTACT 
CCTGGACGAG ACTCCAAAAC TGTCCAAAAG GGATCAGAAA GTGGGCGTGG GAGGCAAAAG 
TCTCCTGCCC AGAGTGACAG CACAGCACAG AGGAGAACTG TAGGGAAAAA ACAACCCAAA 
AAGACTGAGA AGGCAGCTGC TGAAGAGCCC CGTGGAGGCC TGAAGATAGA GAGTGAAACT 
CCTGTAGACA TGGCTACCAG CATGCCCTCC AGCAGACACA AAGCGGCCAC CAAAGGCTCA 
AGAAAACCCA ATATAAAAAA GGAGTCCAAA TCTTCCCCTC GACCTTCAGC AGAGAAAAAG 
AAATACAAGT CAACAAGTAA ATCTTCCCAG AAATCAAGGG AAATTATAGA AACAGATACC 
TCATCCTCAG ATTCGGATGA AAGCGAGAGC CTTCCTCCTT CCTCACAAAC TCCTAAGTAC 
CCTGAGAGTA ATAGGACTCC CGTTAAACCC TCCTCAGTGG AGGAAGAAGA TAGCTTTTTT 
CGGCAACGAA TGTTCTCTCC TATGGAAGAG AAGGAACTTC TTTCACCCCT CAGTGAGCCT 
GATGACAGGT ATCCACTTAT TGTGAAGATT GACCTGAATC TCTTGACTAG AATACCAGGA 
AAGCCTTACA AAGAAACAGA GCCACCCAAG GGGGAAAAGA AAAATGTGCC AGAAAAGCAC 
ACAAGAGAGG CTCAGAAACA AGCCTCAGAG AAAGTTTCCA ACAAAGGCAA GAGGAAACAT 
AAGAATGAAG ATGATAACCG AGCCAATGAA AGCAAGAAAC CTAAAACAGA GGACAAGAAT 
ATGGCAGGCC ACAAGCCATC CAGCAACAAA GAGTCATCTA AACAGAGTGC TTCTAAAGAA 
AAAGATTTAT TGCCTTCTCC TGCTGGGCCT GTTCCTTCAA AAGATCCAAA AACAGAGCAT 
GGCTCTCGGA AGAGGACTGT TAGTCAGTCT TCTTCCTTAA AGTCAAGTAA TAACAGCAAC 
AAGGAAAATA GTGCCAGCAG CAAAAACAGT TCCTCCGCAT CAAAGCAGAA GAGGACTGAA 
GGGAAGACTT CCAGTAGCTC TAAGGAGGTC AAGGAAAAAG CTCCAAATAG TTCTTCTAAC 
TGTCCTCCAT CTGCACCAAC TCCTGATGCT TCTAAGCCTC GGAGAACGAA GCTTGCCTTT 
GATGACAGAA ATTATTCAGC AGACCATTAT TTACAAGAAG CAAAAAAGCT AAAGCACAAT 
GCAGATGCAT TGTCTGATAG GTTTGAGAAA GCTGTATACT ATCTTGATGC TGTGGTATCT 
TTCATTGAAT GTGGGAATGC ATTAGAGAAA AATGCTCAGG AATCCAAATC CCCATTCCTT 
ATGTATTCAG AAACGGTGGA GCTCATCAAA TACACTATGA AGCTAAAGAA TTACTTGGCA 
CCAGATGCTA CAGCTGCAGA TAAAAGACTT ACAGTGCTTT GCCTGCGATG CCAGTCTTTG 
CTGTACCTGA GGCTCTTCAA ACTGAAGAAA GAAACTGCTC TGAAGTATTC AAAGACACTG 
ACAGAGCATC TGAAGAATTC TTACAATAAC TCTCAAGCAC CATCACCAGG CTTGGGAAGC 
AAAGCTGTTG GGATGCCTTC CCCTGTTTCT CCAAAGCTGT CACCAGGCAA TTCAGGAAAT 
TATTCTTCTG GAGCCAGTAG TGCTTCAGCA AGTGGTTCTT CAGTGACCAT TCCACAGAGG 
ATTCACCACA TGGCAGCCAG CTATGTTCAG GTTACATCCA ACTTCCTCTA TGCCACTGAA 
ATTTGGGACC AAGCTGAACA GCTTTCCAAA GAGCAAAAAG AATTCTTTGC TGAACTGGAT 
AAAGTAATGG GCCCTCTCAT CTTTAATGCA AGCATCATGA CAGACCTAGT TCGTTACACC 
CGGCAGGGAC TGCACTGGCT TCGCCAGGAT GCCAAGTTGA TATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805556.1
CDS	367..3774
Translation	MNREDRNVLRMKERERRNQEIQQGDDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPTADEKSNPNFFEQRHGSSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRASAGASGGTNSSGQRHDRDSYSSGGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLSSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSNQSFPPSLMSKSSSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKESQQSSFGPGEQKRYNPSSKTSNAHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQGTGNSYTDPGGPKETSSATPGRDSKTVQKGSESGRGRQKSPAQSDSTAQRRTVGKKQPKKTEKAAAEEPRGGLKIESETPVDMATSMPSSRHKAATKGSRKPNIKKESKSSPRPSAEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRANESKKPKTEDKNMAGHKPSSNKESSKQSASKEKDLLPSPAGPVPSKDPKTEHGSRKRTVSQSSSLKSSNNSNKENSASSKNSSSASKQKRTEGKTSSSSKEVKEKAPNSSSNCPPSAPTPDASKPRRTKLAFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFLMYSETVELIKYTMKLKNYLAPDATAADKRLTVLCLRCQSLLYLRLFKLKKETALKYSKTLTEHLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQRIHHMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	XM_010807254.2
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807254.2

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG 
AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT 
CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC 
AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG 
AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT 
TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT 
GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT 
AAAGAGTCTC AGCAGTCCAG TTTTGGCCCT GGAGAACAAA AAAGATATAA TCCTTCTTCT 
AAAACTTCAA ATGCACACCA GTCTAAATCT ATGTTAAAAG ATGACTTAAA GCTAAGCAGC 
AGTGAAGACA GTGATGGAGA ACAGGATTGT GATAAGACAA TGCCAAGGAG TACACCAGGA 
AGTAACTCTG AACCTTCACA CCATAATAGT GAAGGGGCAG ATAACTCCAG GGATGATTCT 
AGCAGCCACA GTGGATCTGA AAGCAGCTCT GGATCTGACT CAGAGAGTGA AAGTAGTTCC 
AGTGACAGTG AGGCAAATGA GCCATCCCAG AGTGCATCTC CTGAGCCTGA ACCACCTCCC 
ACAAATAAAT GGCAACTTGA TAACTGGCTG AATAAAGTAA ATCCACATAA AGTATCACCA 
GCTTCTTCTG TGGACAGTAA TATCCCATCA TCTCAAGGCT ATAAAAAGGA AGGCCGAGAA 
CAGGGCACAG GGAATAGCTA CACAGATCCA GGTGGTCCTA AAGAAACAAG TTCTGCTACT 
CCTGGACGAG ACTCCAAAAC TGTCCAAAAG GGATCAGAAA GTGGGCGTGG GAGGCAAAAG 
TCTCCTGCCC AGAGTGACAG CACAGCACAG AGGAGAACTG TAGGGAAAAA ACAACCCAAA 
AAGACTGAGA AGGCAGCTGC TGAAGAGCCC CGTGGAGGCC TGAAGATAGA GAGTGAAACT 
CCTGTAGACA TGGCTACCAG CATGCCCTCC AGCAGACACA AAGCGGCCAC CAAAGGCTCA 
AGAAAACCCA ATATAAAAAA GGAGTCCAAA TCTTCCCCTC GACCTTCAGC AGAGAAAAAG 
AAATACAAGT CAACAAGTAA ATCTTCCCAG AAATCAAGGG AAATTATAGA AACAGATACC 
TCATCCTCAG ATTCGGATGA AAGCGAGAGC CTTCCTCCTT CCTCACAAAC TCCTAAGTAC 
CCTGAGAGTA ATAGGACTCC CGTTAAACCC TCCTCAGTGG AGGAAGAAGA TAGCTTTTTT 
CGGCAACGAA TGTTCTCTCC TATGGAAGAG AAGGAACTTC TTTCACCCCT CAGTGAGCCT 
GATGACAGGT ATCCACTTAT TGTGAAGATT GACCTGAATC TCTTGACTAG AATACCAGGA 
AAGCCTTACA AAGAAACAGA GCCACCCAAG GGGGAAAAGA AAAATGTGCC AGAAAAGCAC 
ACAAGAGAGG CTCAGAAACA AGCCTCAGAG AAAGTTTCCA ACAAAGGCAA GAGGAAACAT 
AAGAATGAAG ATGATAACCG AGCCAATGAA AGCAAGAAAC CTAAAACAGA GGACAAGAAT 
ATGGCAGGCC ACAAGCCATC CAGCAACAAA GAGTCATCTA AACAGAGTGC TTCTAAAGAA 
AAAGATTTAT TGCCTTCTCC TGCTGGGCCT GTTCCTTCAA AAGATCCAAA AACAGAGCAT 
GGCTCTCGGA AGAGGACTGT TAGTCAGTCT TCTTCCTTAA AGTCAAGTAA TAACAGCAAC 
AAGGAAAATA GTGCCAGCAG CAAAAACAGT TCCTCCGCAT CAAAGCAGAA GAGGACTGAA 
GGGAAGACTT CCAGTAGCTC TAAGGAGGTC AAGGAAAAAG CTCCAAATAG TTCTTCTAAC 
TGTCCTCCAT CTGCACCAAC TCCTGATGCT TCTAAGCCTC GGAGAACGAA GCTTGCCTTT 
GATGACAGAA ATTATTCAGC AGACCATTAT TTACAAGAAG CAAAAAAGCT AAAGCACAAT 
GCAGATGCAT TGTCTGATAG GTTTGAGAAA GCTGTATACT ATCTTGATGC TGTGGTATCT 
TTCATTGAAT GTGGGAATGC ATTAGAGAAA AATGCTCAGG AATCCAAATC CCCATTCCTT 
ATGTATTCAG AAACGGTGGA GCTCATCAAA TACACTATGA AGCTAAAGAA TTACTTGGCA 
CCAGATGCTA CAGCTGCAGA TAAAAGACTT ACAGTGCTTT GCCTGCGATG CCAGTCTTTG 
CTGTACCTGA GGCTCTTCAA ACTGAAGAAA GAAACTGCTC TGAAGTATTC AAAGACACTG 
ACAGAGCATC TGAAGAATTC TTACAATAAC TCTCAAGCAC CATCACCAGG CTTGGGAAGC 
AAAGCTGTTG GGATGCCTTC CCCTGTTTCT CCAAAGCTGT CACCAGGCAA TTCAGGAAAT 
TATTCTTCTG GAGCCAGTAG TGCTTCAGCA AGTGGTTCTT CAGTGACCAT TCCACAGAGG 
ATTCACCACA TGGCAGCCAG CTATGTTCAG GTTACATCCA ACTTCCTCTA TGCCACTGAA 
ATTTGGGACC AAGCTGAACA GCTTTCCAAA GAGCAAAAAG AATTCTTTGC TGAACTGGAT 
AAAGTAATGG GCCCTCTCAT CTTTAATGCA AGCATCATGA CAGACCTAGT TCGTTACACC 
CGGCAGGGAC TGCACTGGCT TCGCCAGGAT GCCAAGTTGA TATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_010807254.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805556.1 Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X3, mRNA.

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CloneID	OBa21435
Clone ID Related Accession (Same CDS sequence)	XM_010807254.2 , XM_010807254.3
Accession Version	XM_010807254.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807254.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG 
AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT 
CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC 
AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG 
AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT 
TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT 
GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT 
AAAGAGTCTC AGCAGTCCAG TTTTGGCCCT GGAGAACAAA AAAGATATAA TCCTTCTTCT 
AAAACTTCAA ATGCACACCA GTCTAAATCT ATGTTAAAAG ATGACTTAAA GCTAAGCAGC 
AGTGAAGACA GTGATGGAGA ACAGGATTGT GATAAGACAA TGCCAAGGAG TACACCAGGA 
AGTAACTCTG AACCTTCACA CCATAATAGT GAAGGGGCAG ATAACTCCAG GGATGATTCT 
AGCAGCCACA GTGGATCTGA AAGCAGCTCT GGATCTGACT CAGAGAGTGA AAGTAGTTCC 
AGTGACAGTG AGGCAAATGA GCCATCCCAG AGTGCATCTC CTGAGCCTGA ACCACCTCCC 
ACAAATAAAT GGCAACTTGA TAACTGGCTG AATAAAGTAA ATCCACATAA AGTATCACCA 
GCTTCTTCTG TGGACAGTAA TATCCCATCA TCTCAAGGCT ATAAAAAGGA AGGCCGAGAA 
CAGGGCACAG GGAATAGCTA CACAGATCCA GGTGGTCCTA AAGAAACAAG TTCTGCTACT 
CCTGGACGAG ACTCCAAAAC TGTCCAAAAG GGATCAGAAA GTGGGCGTGG GAGGCAAAAG 
TCTCCTGCCC AGAGTGACAG CACAGCACAG AGGAGAACTG TAGGGAAAAA ACAACCCAAA 
AAGACTGAGA AGGCAGCTGC TGAAGAGCCC CGTGGAGGCC TGAAGATAGA GAGTGAAACT 
CCTGTAGACA TGGCTACCAG CATGCCCTCC AGCAGACACA AAGCGGCCAC CAAAGGCTCA 
AGAAAACCCA ATATAAAAAA GGAGTCCAAA TCTTCCCCTC GACCTTCAGC AGAGAAAAAG 
AAATACAAGT CAACAAGTAA ATCTTCCCAG AAATCAAGGG AAATTATAGA AACAGATACC 
TCATCCTCAG ATTCGGATGA AAGCGAGAGC CTTCCTCCTT CCTCACAAAC TCCTAAGTAC 
CCTGAGAGTA ATAGGACTCC CGTTAAACCC TCCTCAGTGG AGGAAGAAGA TAGCTTTTTT 
CGGCAACGAA TGTTCTCTCC TATGGAAGAG AAGGAACTTC TTTCACCCCT CAGTGAGCCT 
GATGACAGGT ATCCACTTAT TGTGAAGATT GACCTGAATC TCTTGACTAG AATACCAGGA 
AAGCCTTACA AAGAAACAGA GCCACCCAAG GGGGAAAAGA AAAATGTGCC AGAAAAGCAC 
ACAAGAGAGG CTCAGAAACA AGCCTCAGAG AAAGTTTCCA ACAAAGGCAA GAGGAAACAT 
AAGAATGAAG ATGATAACCG AGCCAATGAA AGCAAGAAAC CTAAAACAGA GGACAAGAAT 
ATGGCAGGCC ACAAGCCATC CAGCAACAAA GAGTCATCTA AACAGAGTGC TTCTAAAGAA 
AAAGATTTAT TGCCTTCTCC TGCTGGGCCT GTTCCTTCAA AAGATCCAAA AACAGAGCAT 
GGCTCTCGGA AGAGGACTGT TAGTCAGTCT TCTTCCTTAA AGTCAAGTAA TAACAGCAAC 
AAGGAAAATA GTGCCAGCAG CAAAAACAGT TCCTCCGCAT CAAAGCAGAA GAGGACTGAA 
GGGAAGACTT CCAGTAGCTC TAAGGAGGTC AAGGAAAAAG CTCCAAATAG TTCTTCTAAC 
TGTCCTCCAT CTGCACCAAC TCCTGATGCT TCTAAGCCTC GGAGAACGAA GCTTGCCTTT 
GATGACAGAA ATTATTCAGC AGACCATTAT TTACAAGAAG CAAAAAAGCT AAAGCACAAT 
GCAGATGCAT TGTCTGATAG GTTTGAGAAA GCTGTATACT ATCTTGATGC TGTGGTATCT 
TTCATTGAAT GTGGGAATGC ATTAGAGAAA AATGCTCAGG AATCCAAATC CCCATTCCTT 
ATGTATTCAG AAACGGTGGA GCTCATCAAA TACACTATGA AGCTAAAGAA TTACTTGGCA 
CCAGATGCTA CAGCTGCAGA TAAAAGACTT ACAGTGCTTT GCCTGCGATG CCAGTCTTTG 
CTGTACCTGA GGCTCTTCAA ACTGAAGAAA GAAACTGCTC TGAAGTATTC AAAGACACTG 
ACAGAGCATC TGAAGAATTC TTACAATAAC TCTCAAGCAC CATCACCAGG CTTGGGAAGC 
AAAGCTGTTG GGATGCCTTC CCCTGTTTCT CCAAAGCTGT CACCAGGCAA TTCAGGAAAT 
TATTCTTCTG GAGCCAGTAG TGCTTCAGCA AGTGGTTCTT CAGTGACCAT TCCACAGAGG 
ATTCACCACA TGGCAGCCAG CTATGTTCAG GTTACATCCA ACTTCCTCTA TGCCACTGAA 
ATTTGGGACC AAGCTGAACA GCTTTCCAAA GAGCAAAAAG AATTCTTTGC TGAACTGGAT 
AAAGTAATGG GCCCTCTCAT CTTTAATGCA AGCATCATGA CAGACCTAGT TCGTTACACC 
CGGCAGGGAC TGCACTGGCT TCGCCAGGAT GCCAAGTTGA TATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805556.1
CDS	364..3771
Translation	MNREDRNVLRMKERERRNQEIQQGDDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPTADEKSNPNFFEQRHGSSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRASAGASGGTNSSGQRHDRDSYSSGGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLSSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSNQSFPPSLMSKSSSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKESQQSSFGPGEQKRYNPSSKTSNAHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQGTGNSYTDPGGPKETSSATPGRDSKTVQKGSESGRGRQKSPAQSDSTAQRRTVGKKQPKKTEKAAAEEPRGGLKIESETPVDMATSMPSSRHKAATKGSRKPNIKKESKSSPRPSAEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRANESKKPKTEDKNMAGHKPSSNKESSKQSASKEKDLLPSPAGPVPSKDPKTEHGSRKRTVSQSSSLKSSNNSNKENSASSKNSSSASKQKRTEGKTSSSSKEVKEKAPNSSSNCPPSAPTPDASKPRRTKLAFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFLMYSETVELIKYTMKLKNYLAPDATAADKRLTVLCLRCQSLLYLRLFKLKKETALKYSKTLTEHLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQRIHHMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS

Target ORF information:

RefSeq Version	XM_010807254.3
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807254.3

ORF Insert Sequence:

ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA 
GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT 
GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT 
ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG 
AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT 
CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC 
AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG 
AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC 
CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT 
GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG 
GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT 
TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG 
GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT 
GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA 
CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT 
AAAGAGTCTC AGCAGTCCAG TTTTGGCCCT GGAGAACAAA AAAGATATAA TCCTTCTTCT 
AAAACTTCAA ATGCACACCA GTCTAAATCT ATGTTAAAAG ATGACTTAAA GCTAAGCAGC 
AGTGAAGACA GTGATGGAGA ACAGGATTGT GATAAGACAA TGCCAAGGAG TACACCAGGA 
AGTAACTCTG AACCTTCACA CCATAATAGT GAAGGGGCAG ATAACTCCAG GGATGATTCT 
AGCAGCCACA GTGGATCTGA AAGCAGCTCT GGATCTGACT CAGAGAGTGA AAGTAGTTCC 
AGTGACAGTG AGGCAAATGA GCCATCCCAG AGTGCATCTC CTGAGCCTGA ACCACCTCCC 
ACAAATAAAT GGCAACTTGA TAACTGGCTG AATAAAGTAA ATCCACATAA AGTATCACCA 
GCTTCTTCTG TGGACAGTAA TATCCCATCA TCTCAAGGCT ATAAAAAGGA AGGCCGAGAA 
CAGGGCACAG GGAATAGCTA CACAGATCCA GGTGGTCCTA AAGAAACAAG TTCTGCTACT 
CCTGGACGAG ACTCCAAAAC TGTCCAAAAG GGATCAGAAA GTGGGCGTGG GAGGCAAAAG 
TCTCCTGCCC AGAGTGACAG CACAGCACAG AGGAGAACTG TAGGGAAAAA ACAACCCAAA 
AAGACTGAGA AGGCAGCTGC TGAAGAGCCC CGTGGAGGCC TGAAGATAGA GAGTGAAACT 
CCTGTAGACA TGGCTACCAG CATGCCCTCC AGCAGACACA AAGCGGCCAC CAAAGGCTCA 
AGAAAACCCA ATATAAAAAA GGAGTCCAAA TCTTCCCCTC GACCTTCAGC AGAGAAAAAG 
AAATACAAGT CAACAAGTAA ATCTTCCCAG AAATCAAGGG AAATTATAGA AACAGATACC 
TCATCCTCAG ATTCGGATGA AAGCGAGAGC CTTCCTCCTT CCTCACAAAC TCCTAAGTAC 
CCTGAGAGTA ATAGGACTCC CGTTAAACCC TCCTCAGTGG AGGAAGAAGA TAGCTTTTTT 
CGGCAACGAA TGTTCTCTCC TATGGAAGAG AAGGAACTTC TTTCACCCCT CAGTGAGCCT 
GATGACAGGT ATCCACTTAT TGTGAAGATT GACCTGAATC TCTTGACTAG AATACCAGGA 
AAGCCTTACA AAGAAACAGA GCCACCCAAG GGGGAAAAGA AAAATGTGCC AGAAAAGCAC 
ACAAGAGAGG CTCAGAAACA AGCCTCAGAG AAAGTTTCCA ACAAAGGCAA GAGGAAACAT 
AAGAATGAAG ATGATAACCG AGCCAATGAA AGCAAGAAAC CTAAAACAGA GGACAAGAAT 
ATGGCAGGCC ACAAGCCATC CAGCAACAAA GAGTCATCTA AACAGAGTGC TTCTAAAGAA 
AAAGATTTAT TGCCTTCTCC TGCTGGGCCT GTTCCTTCAA AAGATCCAAA AACAGAGCAT 
GGCTCTCGGA AGAGGACTGT TAGTCAGTCT TCTTCCTTAA AGTCAAGTAA TAACAGCAAC 
AAGGAAAATA GTGCCAGCAG CAAAAACAGT TCCTCCGCAT CAAAGCAGAA GAGGACTGAA 
GGGAAGACTT CCAGTAGCTC TAAGGAGGTC AAGGAAAAAG CTCCAAATAG TTCTTCTAAC 
TGTCCTCCAT CTGCACCAAC TCCTGATGCT TCTAAGCCTC GGAGAACGAA GCTTGCCTTT 
GATGACAGAA ATTATTCAGC AGACCATTAT TTACAAGAAG CAAAAAAGCT AAAGCACAAT 
GCAGATGCAT TGTCTGATAG GTTTGAGAAA GCTGTATACT ATCTTGATGC TGTGGTATCT 
TTCATTGAAT GTGGGAATGC ATTAGAGAAA AATGCTCAGG AATCCAAATC CCCATTCCTT 
ATGTATTCAG AAACGGTGGA GCTCATCAAA TACACTATGA AGCTAAAGAA TTACTTGGCA 
CCAGATGCTA CAGCTGCAGA TAAAAGACTT ACAGTGCTTT GCCTGCGATG CCAGTCTTTG 
CTGTACCTGA GGCTCTTCAA ACTGAAGAAA GAAACTGCTC TGAAGTATTC AAAGACACTG 
ACAGAGCATC TGAAGAATTC TTACAATAAC TCTCAAGCAC CATCACCAGG CTTGGGAAGC 
AAAGCTGTTG GGATGCCTTC CCCTGTTTCT CCAAAGCTGT CACCAGGCAA TTCAGGAAAT 
TATTCTTCTG GAGCCAGTAG TGCTTCAGCA AGTGGTTCTT CAGTGACCAT TCCACAGAGG 
ATTCACCACA TGGCAGCCAG CTATGTTCAG GTTACATCCA ACTTCCTCTA TGCCACTGAA 
ATTTGGGACC AAGCTGAACA GCTTTCCAAA GAGCAAAAAG AATTCTTTGC TGAACTGGAT 
AAAGTAATGG GCCCTCTCAT CTTTAATGCA AGCATCATGA CAGACCTAGT TCGTTACACC 
CGGCAGGGAC TGCACTGGCT TCGCCAGGAT GCCAAGTTGA TATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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AFF4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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