SPECC1L cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPECC1L
Entrez Gene ID	23384
Full Name	sperm antigen with calponin homology and coiled-coil domains 1 like
Synonyms	CYTSA,GBBB2,OBLFC1
General protein information	Preferred Names sperm antigen with calponin homology and coiled-coil domains 1 like Names cytospin-A SPECC1-like protein cytokinesis and spindle organization A renal carcinoma antigen NY-REN-22
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q11.23
Summary	This gene encodes a coiled-coil domain containing protein. The encoded protein may play a critical role in actin-cytoskeletal reorganization during facial morphogenesis. Mutations in this gene are a cause of oblique facial clefting-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A read-through transcript composed of SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and the downstream ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013].
Disorder MIM:	614140
Disorder Html:	?Facial clefting, oblique, 1, 600251 (3); Opitz GBBB syndrome, type II, 145410 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001145468.3	NP_001138940.3	cytospin-A isoform 1
NM_015330.4	NP_056145.4	cytospin-A isoform 1
NM_001254733.1	NP_001241662.1	cytospin-A isoform 3
NM_001254732.2	NP_001241661.2	cytospin-A isoform 2
NM_015330.5	NP_056145.4	cytospin-A isoform 1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SPECC1L	NP_001038102.1
Homo sapiens (human)	SPECC1L	NP_001138940.3
Pan troglodytes (chimpanzee)	SPECC1L	NP_001035052.1
Bos taurus (cattle)	SPECC1L	NP_001192793.1
Canis lupus familiaris (dog)	SPECC1L	NP_001041579.1
Mus musculus (house mouse)	Specc1l	NP_001139298.1
Xenopus tropicalis (tropical clawed frog)	specc1l	NP_001037875.1
	SPECC1L	XP_001094607.1
Rattus norvegicus (Norway rat)	Specc1l	NP_001034544.1
Danio rerio (zebrafish)	specc1la	NP_001034905.1

Gene Ontology
Bibliography

Related articles in PubMed

SPECC1L deficiency results in increased adherens junction stability and reduced cranial neural crest cell delamination.
Wilson NR, Olm-Shipman AJ, Acevedo DS, Palaniyandi K, Hall EG, Kosa E, Stumpff KM, Smith GJ, Pitstick L, Liao EC, Bjork BC, Czirok A, Saadi I
Scientific reports617735(2016 Jan)

Expanding the SPECC1L mutation phenotypic spectrum to include Teebi hypertelorism syndrome.
Bhoj EJ, Li D, Harr MH, Tian L, Wang T, Zhao Y, Qiu H, Kim C, Hoffman JD, Hakonarson H, Zackai EH
American journal of medical genetics. Part A167A(11)2497-502(2015 Nov)

Mutations in SPECC1L, encoding sperm antigen with calponin homology and coiled-coil domains 1-like, are found in some cases of autosomal dominant Opitz G/BBB syndrome.
Kruszka P, Li D, Harr MH, Wilson NR, Swarr D, McCormick EM, Chiavacci RM, Li M, Martinez AF, Hart RA, McDonald-McGinn DM, Deardorff MA, Falk MJ, Allanson JE, Hudson C, Johnson JP, Saadi I, Hakonarson H, Muenke M, Zackai EH
Journal of medical genetics52(2)104-10(2015 Feb)

Functional analysis of SPECC1L in craniofacial development and oblique facial cleft pathogenesis.
Gfrerer L, Shubinets V, Hoyos T, Kong Y, Nguyen C, Pietschmann P, Morton CC, Maas RL, Liao EC
Plastic and reconstructive surgery134(4)748-59(2014 Oct)

Genome-wide association study identifies three novel susceptibility loci for severe Acne vulgaris.
Navarini AA, Simpson MA, Weale M, Knight J, Carlavan I, Reiniche P, Burden DA, Layton A, Bataille V, Allen M, Pleass R, Pink A, Creamer D, English J, Munn S, Walton S, , Willis C, D?ret S, Voegel JJ, Spector T, Smith CH, Trembath RC, Barker JN
Nature communications54020(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPECC1L as a novel modulator of PI3K-AKT signaling and AJ biology, required for neural tube closure and CNCC delamination.
Title: SPECC1L deficiency results in increased adherens junction stability and reduced cranial neural crest cell delamination.

two unrelated families with a Teebi hypertelorism-like syndrome and Teebi hypertelorism phenotype who have missense mutations in Sperm Antigen With Calponin Homology And Coiled-Coil Domains (SPECC1L), are reported.
Title: Expanding the SPECC1L mutation phenotypic spectrum to include Teebi hypertelorism syndrome.

SPECC1L mutations can cause syndromic forms of facial clefting including some cases of autosomal dominant Opitz G/BBB syndrome.
Title: Mutations in SPECC1L, encoding sperm antigen with calponin homology and coiled-coil domains 1-like, are found in some cases of autosomal dominant Opitz G/BBB syndrome.

The authors confirm the role of SPECC1L in orofacial cleft pathogenesis in the first animal model of Tessier cleft, providing morphogenetic insight into the mechanisms of normal craniofacial development and oblique facial cleft pathogenesis
Title: Functional analysis of SPECC1L in craniofacial development and oblique facial cleft pathogenesis.

The authors use rapid amplification of cDNA ends, tiling arrays, and deep RNA sequencing to identify chimeric transcripts on human chromosomes 21 and 22. They found that for 492 protein coding genes studied, 85% of these genes had boundaries that extended beyond the current annotated termini.
Title: Evidence for transcript networks composed of chimeric RNAs in human cells.

The following SPECC1L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPECC1L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08245	NM_001254733.1 Latest version!	Homo sapiens sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu00640	NM_015330.5 Latest version!	Homo sapiens sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu00640	NM_001145468.3 Latest version!	Homo sapiens sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu02602	NM_001254732.2 Latest version!	Homo sapiens sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu00640	NM_015330.4	Homo sapiens sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPECC1L ( NM_001254733.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001241662.1 Homo sapiens sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu08245
Clone ID Related Accession (Same CDS sequence)	NM_001254733.1
Accession Version	NM_001254733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	cytospin-A isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC068611.1, AP000354.1, CN334545.1, BC021132.1, BM126792.1 and AA773104.1. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC068611.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAGTG CCAAGACCCT CTCAGTGTCT CGACGAAGTA GTGAAGAAAT GAAACGGGAC 
ATTTCTGCAC AGGAGGGAGC GTCGCCAGCC TCTCTGATGG CTATGGGAAC CACGTCTCCA 
CAGCTTTCCC TGTCCTCTTC TCCAACGGCA TCTGTGACTC CCACCACCCG AAGCCGAATA 
AGAGAAGAAA GGAAAGACCC TCTCTCAGCA TTGGCCAGAG AATATGGAGG ATCAAAGAGG 
AACGCCTTGC TGAAGTGGTG TCAGAAGAAA ACAGAAGGCT ATCAGAATAT TGACATTACA 
AACTTCAGCA GCAGCTGGAA TGATGGGCTG GCCTTCTGTG CCCTCCTGCA TACATATCTC 
CCTGCCCACA TTCCATATCA AGAACTGAAC AGCCAGGATA AGAGAAGGAA CTTCATGCTG 
GCTTTCCAGG CAGCTGAAAG TGTCGGCATC AAATCCACAC TGGACATTAA TGAAATGGTA 
CGGACTGAAC GACCCGACTG GCAGAACGTG ATGCTGTATG TGACGGCGAT CTACAAGTAC 
TTTGAGACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001241662.1
CDS	546..1097
Translation	MESAKTLSVSRRSSEEMKRDISAQEGASPASLMAMGTTSPQLSLSSSPTASVTPTTRSRIREERKDPLSALAREYGGSKRNALLKWCQKKTEGYQNIDITNFSSSWNDGLAFCALLHTYLPAHIPYQELNSQDKRRNFMLAFQAAESVGIKSTLDINEMVRTERPDWQNVMLYVTAIYKYFET

Target ORF information:

RefSeq Version	NM_001254733.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001254733.1

ORF Insert Sequence:

ATGGAAAGTG CCAAGACCCT CTCAGTGTCT CGACGAAGTA GTGAAGAAAT GAAACGGGAC 
ATTTCTGCAC AGGAGGGAGC GTCGCCAGCC TCTCTGATGG CTATGGGAAC CACGTCTCCA 
CAGCTTTCCC TGTCCTCTTC TCCAACGGCA TCTGTGACTC CCACCACCCG AAGCCGAATA 
AGAGAAGAAA GGAAAGACCC TCTCTCAGCA TTGGCCAGAG AATATGGAGG ATCAAAGAGG 
AACGCCTTGC TGAAGTGGTG TCAGAAGAAA ACAGAAGGCT ATCAGAATAT TGACATTACA 
AACTTCAGCA GCAGCTGGAA TGATGGGCTG GCCTTCTGTG CCCTCCTGCA TACATATCTC 
CCTGCCCACA TTCCATATCA AGAACTGAAC AGCCAGGATA AGAGAAGGAA CTTCATGCTG 
GCTTTCCAGG CAGCTGAAAG TGTCGGCATC AAATCCACAC TGGACATTAA TGAAATGGTA 
CGGACTGAAC GACCCGACTG GCAGAACGTG ATGCTGTATG TGACGGCGAT CTACAAGTAC 
TTTGAGACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPECC1L ( NM_001145468.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138940.3 Homo sapiens sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu00640
Clone ID Related Accession (Same CDS sequence)	NM_001145468.3 , NM_015330.4 , NM_015330.5
Accession Version	NM_001145468.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	cytospin-A isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY105216.1, BC136471.1, BQ929263.1, BM126792.1 and AA773104.1. On Jun 20, 2013 this sequence version replaced NM_001145468.2. Transcript Variant: This variant (2) lacks an exon in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC136471.1, SRR1660805.146763.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGAAAG CAAGCAGGAG TGTTGGCTCA GTGCCTAAAG TGTCTGCAAT AAGTAAAACG 
CAAACAGCAG AAAAAATTAA ACCTGAAAAC AGCTCTTCAG CATCTACGGG AGGCAAACTT 
GTAAAACCTG GAACAGCAGC ATCATTGTCA AAGACCAAGA GCAGTGATGA CCTTTTAGCT 
GGAATGGCCG GAGGGGTAAC GGTGACTAAT GGTGTTAAAG GAAAGAAAAG CACCTGCCCA 
TCTGCAGCAC CTTCAGCATC TGCCCCTGCC ATGACCACCG TGGAGAACAA ATCCAAGATT 
AGCACAGGCA CAGCTTCTTC AACCAAGCGG AGCACTTCTA CAGGTAATAA AGAATCCAGT 
TCTACTAGAG AAAGATTACG TGAACGTACC CGATTAAACC AGAGCAAAAA ACTACCTTCT 
GCAGGTCAGG GAGCTAATGA CATGGCATTG GCCAAACGTT CCCGCAGTCG AACTGCTACA 
GAATGTGACG TTCGTATGAG CAAGTCTAAG TCAGACAATC AGATCAGTGA CAGAGCTGCT 
TTGGAGGCCA AAGTGAAGGA TCTTCTCACG CTGGCAAAAA CCAAAGACGT AGAAATTTTA 
CATTTGAGAA ATGAACTGCG AGACATGCGT GCCCAGCTGG GCATTAATGA GGATCATTCT 
GAGGGTGATG AAAAATCTGA GAAGGAAACT ATTATGGCTC ACCAGCCGAC TGATGTGGAG 
TCCACTTTAT TGCAGTTGCA GGAACAGAAT ACTGCCATCC GTGAAGAACT CAACCAGCTG 
AAAAATGAAA ACAGAATGTT AAAGGACAGG TTGAATGCAT TGGGCTTTTC CCTAGAGCAG 
AGGTTAGACA ATTCTGAAAA ACTGTTTGGC TATCAGTCCC TGAGCCCAGA AATCACCCCT 
GGTAACCAGA GCGATGGAGG AGGAACTCTG ACTTCTTCAG TGGAAGGCTC TGCCCCTGGC 
TCAGTGGAGG ATCTCTTGAG TCAGGATGAA AATACACTAA TGGACCATCA GCACAGTAAC 
TCCATGGACA ATTTAGACAG TGAGTGCAGT GAGGTCTACC AGCCCCTCAC ATCGAGCGAT 
GATGCGCTGG ATGCACCATC CTCCTCAGAG TCGGAAGGCA TCCCCAGCAT AGAGCGCTCC 
CGGAAGGGGA GCAGCGGGAA TGCCAGTGAA GTGTCCGTGG CTTGCCTGAC TGAACGGATA 
CACCAGATGG AAGAGAACCA ACACAGTACA AGTGAGGAAC TCCAGGCAAC CCTGCAAGAG 
CTAGCTGATT TACAGCAGAT TACCCAGGAA CTGAATAGTG AAAACGAAAG GCTTGGAGAA 
GAGAAGGTTA TTCTGATGGA GTCTTTATGT CAGCAGAGCG ATAAGTTGGA ACACTTTAGT 
CGACAGATTG AATACTTCCG CTCTCTTCTA GATGAGCATC ACATTTCTTA TGTCATAGAT 
GAAGATGTAA AAAGTGGGCG CTATATGGAA TTAGAGCAAC GTTACATGGA CCTCGCTGAG 
AATGCCCGTT TTGAACGGGA GCAGCTTCTT GGTGTCCAGC AGCATTTAAG CAATACTTTG 
AAAATGGCAG AACAAGACAA TAAGGAAGCT CAAGAAATGA TAGGGGCACT CAAAGAACGC 
AGTCACCATA TGGAGCGAAT TATTGAGTCT GAGCAGAAAG GAAAAGCAGC CTTGGCAGCC 
ACGTTAGAGG AATACAAAGC CACAGTGGCC AGTGACCAGA TAGAGATGAA TCGCCTGAAG 
GCTCAGCTGG AGAATGAAAA GCAGAAAGTG GCAGAGCTGT ATTCTATCCA TAACTCTGGA 
GACAAATCTG ATATTCAGGA CCTCCTGGAG AGTGTCAGGC TGGACAAAGA AAAAGCAGAG 
ACTTTGGCTA GTAGCTTGCA GGAAGATCTG GCTCATACCC GAAATGATGC CAATCGATTA 
CAGGATGCCA TTGCTAAGGT AGAGGATGAA TACCGAGCCT TCCAAGAAGA AGCTAAGAAA 
CAAATTGAAG ATTTGAATAT GACGTTAGAA AAATTAAGAT CAGACCTGGA TGAAAAAGAA 
ACAGAAAGGA GTGACATGAA AGAAACCATC TTTGAACTTG AAGATGAAGT AGAACAACAT 
CGTGCTGTGA AACTTCATGA CAACCTCATT ATTTCTGATC TAGAGAATAC AGTTAAAAAA 
CTCCAGGACC AAAAGCACGA CATGGAAAGA GAAATAAAGA CACTCCACAG AAGACTTCGG 
GAAGAATCTG CGGAATGGCG GCAGTTTCAG GCTGATCTCC AGACTGCAGT AGTCATTGCA 
AATGACATTA AATCTGAAGC CCAAGAGGAG ATTGGTGATC TAAAGCGCCG GTTACATGAG 
GCTCAAGAAA AAAATGAGAA ACTCACAAAA GAATTGGAGG AAATAAAGTC ACGCAAGCAA 
GAGGAGGAGC GAGGCCGGGT ATACAATTAC ATGAATGCCG TTGAGAGAGA TTTGGCAGCC 
TTAAGGCAGG GAATGGGACT GAGTAGAAGG TCCTCGACTT CCTCAGAGCC AACTCCTACA 
GTAAAAACCC TCATCAAGTC CTTTGACAGT GCATCTCAAG TACCAAACCC TGCTGCAGCT 
GCAATTCCTC GAACGCCCCT GAGCCCAAGT CCTATGAAAA CCCCTCCTGC AGCAGCTGTG 
TCCCCTATGC AGAGACATTC CATAAGTGGA CCAATCTCAA CATCCAAACC CCTGACAGCC 
CTGTCAGATA AGAGACCAAA CTATGGGGAA ATCCCTGTTC AAGAGCATCT GTTAAGAACA 
TCTTCAGCCA GCCGGCCTGC TTCCCTGCCA AGAGTGCCTG CGATGGAAAG TGCCAAGACC 
CTCTCAGTGT CTCGACGAAG TAGTGAAGAA ATGAAACGGG ACATTTCTGC ACAGGAGGGA 
GCGTCGCCAG CCTCTCTGAT GGCTATGGGA ACCACGTCTC CACAGCTTTC CCTGTCCTCT 
TCTCCAACGG CATCTGTGAC TCCCACCACC CGAAGCCGAA TAAGAGAAGA AAGGAAAGAC 
CCTCTCTCAG CATTGGCCAG AGAATATGGA GGATCAAAGA GGAACGCCTT GCTGAAGTGG 
TGTCAGAAGA AAACAGAAGG CTATCAGAAT ATTGACATTA CAAACTTCAG CAGCAGCTGG 
AATGATGGGC TGGCCTTCTG TGCCCTCCTG CATACATATC TCCCTGCCCA CATTCCATAT 
CAAGAACTGA ACAGCCAGGA TAAGAGAAGG AACTTCATGC TGGCTTTCCA GGCAGCTGAA 
AGTGTCGGCA TCAAATCCAC ACTGGACATT AATGAAATGG TACGGACTGA ACGACCCGAC 
TGGCAGAACG TGATGCTGTA TGTGACGGCG ATCTACAAGT ACTTTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138940.3
CDS	205..3558
Translation	MKKASRSVGSVPKVSAISKTQTAEKIKPENSSSASTGGKLVKPGTAASLSKTKSSDDLLAGMAGGVTVTNGVKGKKSTCPSAAPSASAPAMTTVENKSKISTGTASSTKRSTSTGNKESSSTRERLRERTRLNQSKKLPSAGQGANDMALAKRSRSRTATECDVRMSKSKSDNQISDRAALEAKVKDLLTLAKTKDVEILHLRNELRDMRAQLGINEDHSEGDEKSEKETIMAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLEQRLDNSEKLFGYQSLSPEITPGNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSECSEVYQPLTSSDDALDAPSSSESEGIPSIERSRKGSSGNASEVSVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNSENERLGEEKVILMESLCQQSDKLEHFSRQIEYFRSLLDEHHISYVIDEDVKSGRYMELEQRYMDLAENARFEREQLLGVQQHLSNTLKMAEQDNKEAQEMIGALKERSHHMERIIESEQKGKAALAATLEEYKATVASDQIEMNRLKAQLENEKQKVAELYSIHNSGDKSDIQDLLESVRLDKEKAETLASSLQEDLAHTRNDANRLQDAIAKVEDEYRAFQEEAKKQIEDLNMTLEKLRSDLDEKETERSDMKETIFELEDEVEQHRAVKLHDNLIISDLENTVKKLQDQKHDMEREIKTLHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLKRRLHEAQEKNEKLTKELEEIKSRKQEEERGRVYNYMNAVERDLAALRQGMGLSRRSSTSSEPTPTVKTLIKSFDSASQVPNPAAAAIPRTPLSPSPMKTPPAAAVSPMQRHSISGPISTSKPLTALSDKRPNYGEIPVQEHLLRTSSASRPASLPRVPAMESAKTLSVSRRSSEEMKRDISAQEGASPASLMAMGTTSPQLSLSSSPTASVTPTTRSRIREERKDPLSALAREYGGSKRNALLKWCQKKTEGYQNIDITNFSSSWNDGLAFCALLHTYLPAHIPYQELNSQDKRRNFMLAFQAAESVGIKSTLDINEMVRTERPDWQNVMLYVTAIYKYFET

Target ORF information:

RefSeq Version	NM_001145468.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145468.3

ORF Insert Sequence:

ATGAAGAAAG CAAGCAGGAG TGTTGGCTCA GTGCCTAAAG TGTCTGCAAT AAGTAAAACG 
CAAACAGCAG AAAAAATTAA ACCTGAAAAC AGCTCTTCAG CATCTACGGG AGGCAAACTT 
GTAAAACCTG GAACAGCAGC ATCATTGTCA AAGACCAAGA GCAGTGATGA CCTTTTAGCT 
GGAATGGCCG GAGGGGTAAC GGTGACTAAT GGTGTTAAAG GAAAGAAAAG CACCTGCCCA 
TCTGCAGCAC CTTCAGCATC TGCCCCTGCC ATGACCACCG TGGAGAACAA ATCCAAGATT 
AGCACAGGCA CAGCTTCTTC AACCAAGCGG AGCACTTCTA CAGGTAATAA AGAATCCAGT 
TCTACTAGAG AAAGATTACG TGAACGTACC CGATTAAACC AGAGCAAAAA ACTACCTTCT 
GCAGGTCAGG GAGCTAATGA CATGGCATTG GCCAAACGTT CCCGCAGTCG AACTGCTACA 
GAATGTGACG TTCGTATGAG CAAGTCTAAG TCAGACAATC AGATCAGTGA CAGAGCTGCT 
TTGGAGGCCA AAGTGAAGGA TCTTCTCACG CTGGCAAAAA CCAAAGACGT AGAAATTTTA 
CATTTGAGAA ATGAACTGCG AGACATGCGT GCCCAGCTGG GCATTAATGA GGATCATTCT 
GAGGGTGATG AAAAATCTGA GAAGGAAACT ATTATGGCTC ACCAGCCGAC TGATGTGGAG 
TCCACTTTAT TGCAGTTGCA GGAACAGAAT ACTGCCATCC GTGAAGAACT CAACCAGCTG 
AAAAATGAAA ACAGAATGTT AAAGGACAGG TTGAATGCAT TGGGCTTTTC CCTAGAGCAG 
AGGTTAGACA ATTCTGAAAA ACTGTTTGGC TATCAGTCCC TGAGCCCAGA AATCACCCCT 
GGTAACCAGA GCGATGGAGG AGGAACTCTG ACTTCTTCAG TGGAAGGCTC TGCCCCTGGC 
TCAGTGGAGG ATCTCTTGAG TCAGGATGAA AATACACTAA TGGACCATCA GCACAGTAAC 
TCCATGGACA ATTTAGACAG TGAGTGCAGT GAGGTCTACC AGCCCCTCAC ATCGAGCGAT 
GATGCGCTGG ATGCACCATC CTCCTCAGAG TCGGAAGGCA TCCCCAGCAT AGAGCGCTCC 
CGGAAGGGGA GCAGCGGGAA TGCCAGTGAA GTGTCCGTGG CTTGCCTGAC TGAACGGATA 
CACCAGATGG AAGAGAACCA ACACAGTACA AGTGAGGAAC TCCAGGCAAC CCTGCAAGAG 
CTAGCTGATT TACAGCAGAT TACCCAGGAA CTGAATAGTG AAAACGAAAG GCTTGGAGAA 
GAGAAGGTTA TTCTGATGGA GTCTTTATGT CAGCAGAGCG ATAAGTTGGA ACACTTTAGT 
CGACAGATTG AATACTTCCG CTCTCTTCTA GATGAGCATC ACATTTCTTA TGTCATAGAT 
GAAGATGTAA AAAGTGGGCG CTATATGGAA TTAGAGCAAC GTTACATGGA CCTCGCTGAG 
AATGCCCGTT TTGAACGGGA GCAGCTTCTT GGTGTCCAGC AGCATTTAAG CAATACTTTG 
AAAATGGCAG AACAAGACAA TAAGGAAGCT CAAGAAATGA TAGGGGCACT CAAAGAACGC 
AGTCACCATA TGGAGCGAAT TATTGAGTCT GAGCAGAAAG GAAAAGCAGC CTTGGCAGCC 
ACGTTAGAGG AATACAAAGC CACAGTGGCC AGTGACCAGA TAGAGATGAA TCGCCTGAAG 
GCTCAGCTGG AGAATGAAAA GCAGAAAGTG GCAGAGCTGT ATTCTATCCA TAACTCTGGA 
GACAAATCTG ATATTCAGGA CCTCCTGGAG AGTGTCAGGC TGGACAAAGA AAAAGCAGAG 
ACTTTGGCTA GTAGCTTGCA GGAAGATCTG GCTCATACCC GAAATGATGC CAATCGATTA 
CAGGATGCCA TTGCTAAGGT AGAGGATGAA TACCGAGCCT TCCAAGAAGA AGCTAAGAAA 
CAAATTGAAG ATTTGAATAT GACGTTAGAA AAATTAAGAT CAGACCTGGA TGAAAAAGAA 
ACAGAAAGGA GTGACATGAA AGAAACCATC TTTGAACTTG AAGATGAAGT AGAACAACAT 
CGTGCTGTGA AACTTCATGA CAACCTCATT ATTTCTGATC TAGAGAATAC AGTTAAAAAA 
CTCCAGGACC AAAAGCACGA CATGGAAAGA GAAATAAAGA CACTCCACAG AAGACTTCGG 
GAAGAATCTG CGGAATGGCG GCAGTTTCAG GCTGATCTCC AGACTGCAGT AGTCATTGCA 
AATGACATTA AATCTGAAGC CCAAGAGGAG ATTGGTGATC TAAAGCGCCG GTTACATGAG 
GCTCAAGAAA AAAATGAGAA ACTCACAAAA GAATTGGAGG AAATAAAGTC ACGCAAGCAA 
GAGGAGGAGC GAGGCCGGGT ATACAATTAC ATGAATGCCG TTGAGAGAGA TTTGGCAGCC 
TTAAGGCAGG GAATGGGACT GAGTAGAAGG TCCTCGACTT CCTCAGAGCC AACTCCTACA 
GTAAAAACCC TCATCAAGTC CTTTGACAGT GCATCTCAAG TACCAAACCC TGCTGCAGCT 
GCAATTCCTC GAACGCCCCT GAGCCCAAGT CCTATGAAAA CCCCTCCTGC AGCAGCTGTG 
TCCCCTATGC AGAGACATTC CATAAGTGGA CCAATCTCAA CATCCAAACC CCTGACAGCC 
CTGTCAGATA AGAGACCAAA CTATGGGGAA ATCCCTGTTC AAGAGCATCT GTTAAGAACA 
TCTTCAGCCA GCCGGCCTGC TTCCCTGCCA AGAGTGCCTG CGATGGAAAG TGCCAAGACC 
CTCTCAGTGT CTCGACGAAG TAGTGAAGAA ATGAAACGGG ACATTTCTGC ACAGGAGGGA 
GCGTCGCCAG CCTCTCTGAT GGCTATGGGA ACCACGTCTC CACAGCTTTC CCTGTCCTCT 
TCTCCAACGG CATCTGTGAC TCCCACCACC CGAAGCCGAA TAAGAGAAGA AAGGAAAGAC 
CCTCTCTCAG CATTGGCCAG AGAATATGGA GGATCAAAGA GGAACGCCTT GCTGAAGTGG 
TGTCAGAAGA AAACAGAAGG CTATCAGAAT ATTGACATTA CAAACTTCAG CAGCAGCTGG 
AATGATGGGC TGGCCTTCTG TGCCCTCCTG CATACATATC TCCCTGCCCA CATTCCATAT 
CAAGAACTGA ACAGCCAGGA TAAGAGAAGG AACTTCATGC TGGCTTTCCA GGCAGCTGAA 
AGTGTCGGCA TCAAATCCAC ACTGGACATT AATGAAATGG TACGGACTGA ACGACCCGAC 
TGGCAGAACG TGATGCTGTA TGTGACGGCG ATCTACAAGT ACTTTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPECC1L ( NM_015330.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056145.4 Homo sapiens sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00640
Clone ID Related Accession (Same CDS sequence)	NM_001145468.3 , NM_015330.4 , NM_015330.5
Accession Version	NM_015330.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	cytospin-A isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY105216.1, AL832425.1, BC013085.1, BC021132.1, BM126792.1 and AA773104.1. This sequence is a reference standard in the RefSeqGene project. On Jun 20, 2013 this sequence version replaced NM_015330.3. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGAAAG CAAGCAGGAG TGTTGGCTCA GTGCCTAAAG TGTCTGCAAT AAGTAAAACG 
CAAACAGCAG AAAAAATTAA ACCTGAAAAC AGCTCTTCAG CATCTACGGG AGGCAAACTT 
GTAAAACCTG GAACAGCAGC ATCATTGTCA AAGACCAAGA GCAGTGATGA CCTTTTAGCT 
GGAATGGCCG GAGGGGTAAC GGTGACTAAT GGTGTTAAAG GAAAGAAAAG CACCTGCCCA 
TCTGCAGCAC CTTCAGCATC TGCCCCTGCC ATGACCACCG TGGAGAACAA ATCCAAGATT 
AGCACAGGCA CAGCTTCTTC AACCAAGCGG AGCACTTCTA CAGGTAATAA AGAATCCAGT 
TCTACTAGAG AAAGATTACG TGAACGTACC CGATTAAACC AGAGCAAAAA ACTACCTTCT 
GCAGGTCAGG GAGCTAATGA CATGGCATTG GCCAAACGTT CCCGCAGTCG AACTGCTACA 
GAATGTGACG TTCGTATGAG CAAGTCTAAG TCAGACAATC AGATCAGTGA CAGAGCTGCT 
TTGGAGGCCA AAGTGAAGGA TCTTCTCACG CTGGCAAAAA CCAAAGACGT AGAAATTTTA 
CATTTGAGAA ATGAACTGCG AGACATGCGT GCCCAGCTGG GCATTAATGA GGATCATTCT 
GAGGGTGATG AAAAATCTGA GAAGGAAACT ATTATGGCTC ACCAGCCGAC TGATGTGGAG 
TCCACTTTAT TGCAGTTGCA GGAACAGAAT ACTGCCATCC GTGAAGAACT CAACCAGCTG 
AAAAATGAAA ACAGAATGTT AAAGGACAGG TTGAATGCAT TGGGCTTTTC CCTAGAGCAG 
AGGTTAGACA ATTCTGAAAA ACTGTTTGGC TATCAGTCCC TGAGCCCAGA AATCACCCCT 
GGTAACCAGA GCGATGGAGG AGGAACTCTG ACTTCTTCAG TGGAAGGCTC TGCCCCTGGC 
TCAGTGGAGG ATCTCTTGAG TCAGGATGAA AATACACTAA TGGACCATCA GCACAGTAAC 
TCCATGGACA ATTTAGACAG TGAGTGCAGT GAGGTCTACC AGCCCCTCAC ATCGAGCGAT 
GATGCGCTGG ATGCACCATC CTCCTCAGAG TCGGAAGGCA TCCCCAGCAT AGAGCGCTCC 
CGGAAGGGGA GCAGCGGGAA TGCCAGTGAA GTGTCCGTGG CTTGCCTGAC TGAACGGATA 
CACCAGATGG AAGAGAACCA ACACAGTACA AGTGAGGAAC TCCAGGCAAC CCTGCAAGAG 
CTAGCTGATT TACAGCAGAT TACCCAGGAA CTGAATAGTG AAAACGAAAG GCTTGGAGAA 
GAGAAGGTTA TTCTGATGGA GTCTTTATGT CAGCAGAGCG ATAAGTTGGA ACACTTTAGT 
CGACAGATTG AATACTTCCG CTCTCTTCTA GATGAGCATC ACATTTCTTA TGTCATAGAT 
GAAGATGTAA AAAGTGGGCG CTATATGGAA TTAGAGCAAC GTTACATGGA CCTCGCTGAG 
AATGCCCGTT TTGAACGGGA GCAGCTTCTT GGTGTCCAGC AGCATTTAAG CAATACTTTG 
AAAATGGCAG AACAAGACAA TAAGGAAGCT CAAGAAATGA TAGGGGCACT CAAAGAACGC 
AGTCACCATA TGGAGCGAAT TATTGAGTCT GAGCAGAAAG GAAAAGCAGC CTTGGCAGCC 
ACGTTAGAGG AATACAAAGC CACAGTGGCC AGTGACCAGA TAGAGATGAA TCGCCTGAAG 
GCTCAGCTGG AGAATGAAAA GCAGAAAGTG GCAGAGCTGT ATTCTATCCA TAACTCTGGA 
GACAAATCTG ATATTCAGGA CCTCCTGGAG AGTGTCAGGC TGGACAAAGA AAAAGCAGAG 
ACTTTGGCTA GTAGCTTGCA GGAAGATCTG GCTCATACCC GAAATGATGC CAATCGATTA 
CAGGATGCCA TTGCTAAGGT AGAGGATGAA TACCGAGCCT TCCAAGAAGA AGCTAAGAAA 
CAAATTGAAG ATTTGAATAT GACGTTAGAA AAATTAAGAT CAGACCTGGA TGAAAAAGAA 
ACAGAAAGGA GTGACATGAA AGAAACCATC TTTGAACTTG AAGATGAAGT AGAACAACAT 
CGTGCTGTGA AACTTCATGA CAACCTCATT ATTTCTGATC TAGAGAATAC AGTTAAAAAA 
CTCCAGGACC AAAAGCACGA CATGGAAAGA GAAATAAAGA CACTCCACAG AAGACTTCGG 
GAAGAATCTG CGGAATGGCG GCAGTTTCAG GCTGATCTCC AGACTGCAGT AGTCATTGCA 
AATGACATTA AATCTGAAGC CCAAGAGGAG ATTGGTGATC TAAAGCGCCG GTTACATGAG 
GCTCAAGAAA AAAATGAGAA ACTCACAAAA GAATTGGAGG AAATAAAGTC ACGCAAGCAA 
GAGGAGGAGC GAGGCCGGGT ATACAATTAC ATGAATGCCG TTGAGAGAGA TTTGGCAGCC 
TTAAGGCAGG GAATGGGACT GAGTAGAAGG TCCTCGACTT CCTCAGAGCC AACTCCTACA 
GTAAAAACCC TCATCAAGTC CTTTGACAGT GCATCTCAAG TACCAAACCC TGCTGCAGCT 
GCAATTCCTC GAACGCCCCT GAGCCCAAGT CCTATGAAAA CCCCTCCTGC AGCAGCTGTG 
TCCCCTATGC AGAGACATTC CATAAGTGGA CCAATCTCAA CATCCAAACC CCTGACAGCC 
CTGTCAGATA AGAGACCAAA CTATGGGGAA ATCCCTGTTC AAGAGCATCT GTTAAGAACA 
TCTTCAGCCA GCCGGCCTGC TTCCCTGCCA AGAGTGCCTG CGATGGAAAG TGCCAAGACC 
CTCTCAGTGT CTCGACGAAG TAGTGAAGAA ATGAAACGGG ACATTTCTGC ACAGGAGGGA 
GCGTCGCCAG CCTCTCTGAT GGCTATGGGA ACCACGTCTC CACAGCTTTC CCTGTCCTCT 
TCTCCAACGG CATCTGTGAC TCCCACCACC CGAAGCCGAA TAAGAGAAGA AAGGAAAGAC 
CCTCTCTCAG CATTGGCCAG AGAATATGGA GGATCAAAGA GGAACGCCTT GCTGAAGTGG 
TGTCAGAAGA AAACAGAAGG CTATCAGAAT ATTGACATTA CAAACTTCAG CAGCAGCTGG 
AATGATGGGC TGGCCTTCTG TGCCCTCCTG CATACATATC TCCCTGCCCA CATTCCATAT 
CAAGAACTGA ACAGCCAGGA TAAGAGAAGG AACTTCATGC TGGCTTTCCA GGCAGCTGAA 
AGTGTCGGCA TCAAATCCAC ACTGGACATT AATGAAATGG TACGGACTGA ACGACCCGAC 
TGGCAGAACG TGATGCTGTA TGTGACGGCG ATCTACAAGT ACTTTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056145.4
CDS	309..3662
Translation	MKKASRSVGSVPKVSAISKTQTAEKIKPENSSSASTGGKLVKPGTAASLSKTKSSDDLLAGMAGGVTVTNGVKGKKSTCPSAAPSASAPAMTTVENKSKISTGTASSTKRSTSTGNKESSSTRERLRERTRLNQSKKLPSAGQGANDMALAKRSRSRTATECDVRMSKSKSDNQISDRAALEAKVKDLLTLAKTKDVEILHLRNELRDMRAQLGINEDHSEGDEKSEKETIMAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLEQRLDNSEKLFGYQSLSPEITPGNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSECSEVYQPLTSSDDALDAPSSSESEGIPSIERSRKGSSGNASEVSVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNSENERLGEEKVILMESLCQQSDKLEHFSRQIEYFRSLLDEHHISYVIDEDVKSGRYMELEQRYMDLAENARFEREQLLGVQQHLSNTLKMAEQDNKEAQEMIGALKERSHHMERIIESEQKGKAALAATLEEYKATVASDQIEMNRLKAQLENEKQKVAELYSIHNSGDKSDIQDLLESVRLDKEKAETLASSLQEDLAHTRNDANRLQDAIAKVEDEYRAFQEEAKKQIEDLNMTLEKLRSDLDEKETERSDMKETIFELEDEVEQHRAVKLHDNLIISDLENTVKKLQDQKHDMEREIKTLHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLKRRLHEAQEKNEKLTKELEEIKSRKQEEERGRVYNYMNAVERDLAALRQGMGLSRRSSTSSEPTPTVKTLIKSFDSASQVPNPAAAAIPRTPLSPSPMKTPPAAAVSPMQRHSISGPISTSKPLTALSDKRPNYGEIPVQEHLLRTSSASRPASLPRVPAMESAKTLSVSRRSSEEMKRDISAQEGASPASLMAMGTTSPQLSLSSSPTASVTPTTRSRIREERKDPLSALAREYGGSKRNALLKWCQKKTEGYQNIDITNFSSSWNDGLAFCALLHTYLPAHIPYQELNSQDKRRNFMLAFQAAESVGIKSTLDINEMVRTERPDWQNVMLYVTAIYKYFET

Target ORF information:

RefSeq Version	NM_015330.4
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015330.4

ORF Insert Sequence:

ATGAAGAAAG CAAGCAGGAG TGTTGGCTCA GTGCCTAAAG TGTCTGCAAT AAGTAAAACG 
CAAACAGCAG AAAAAATTAA ACCTGAAAAC AGCTCTTCAG CATCTACGGG AGGCAAACTT 
GTAAAACCTG GAACAGCAGC ATCATTGTCA AAGACCAAGA GCAGTGATGA CCTTTTAGCT 
GGAATGGCCG GAGGGGTAAC GGTGACTAAT GGTGTTAAAG GAAAGAAAAG CACCTGCCCA 
TCTGCAGCAC CTTCAGCATC TGCCCCTGCC ATGACCACCG TGGAGAACAA ATCCAAGATT 
AGCACAGGCA CAGCTTCTTC AACCAAGCGG AGCACTTCTA CAGGTAATAA AGAATCCAGT 
TCTACTAGAG AAAGATTACG TGAACGTACC CGATTAAACC AGAGCAAAAA ACTACCTTCT 
GCAGGTCAGG GAGCTAATGA CATGGCATTG GCCAAACGTT CCCGCAGTCG AACTGCTACA 
GAATGTGACG TTCGTATGAG CAAGTCTAAG TCAGACAATC AGATCAGTGA CAGAGCTGCT 
TTGGAGGCCA AAGTGAAGGA TCTTCTCACG CTGGCAAAAA CCAAAGACGT AGAAATTTTA 
CATTTGAGAA ATGAACTGCG AGACATGCGT GCCCAGCTGG GCATTAATGA GGATCATTCT 
GAGGGTGATG AAAAATCTGA GAAGGAAACT ATTATGGCTC ACCAGCCGAC TGATGTGGAG 
TCCACTTTAT TGCAGTTGCA GGAACAGAAT ACTGCCATCC GTGAAGAACT CAACCAGCTG 
AAAAATGAAA ACAGAATGTT AAAGGACAGG TTGAATGCAT TGGGCTTTTC CCTAGAGCAG 
AGGTTAGACA ATTCTGAAAA ACTGTTTGGC TATCAGTCCC TGAGCCCAGA AATCACCCCT 
GGTAACCAGA GCGATGGAGG AGGAACTCTG ACTTCTTCAG TGGAAGGCTC TGCCCCTGGC 
TCAGTGGAGG ATCTCTTGAG TCAGGATGAA AATACACTAA TGGACCATCA GCACAGTAAC 
TCCATGGACA ATTTAGACAG TGAGTGCAGT GAGGTCTACC AGCCCCTCAC ATCGAGCGAT 
GATGCGCTGG ATGCACCATC CTCCTCAGAG TCGGAAGGCA TCCCCAGCAT AGAGCGCTCC 
CGGAAGGGGA GCAGCGGGAA TGCCAGTGAA GTGTCCGTGG CTTGCCTGAC TGAACGGATA 
CACCAGATGG AAGAGAACCA ACACAGTACA AGTGAGGAAC TCCAGGCAAC CCTGCAAGAG 
CTAGCTGATT TACAGCAGAT TACCCAGGAA CTGAATAGTG AAAACGAAAG GCTTGGAGAA 
GAGAAGGTTA TTCTGATGGA GTCTTTATGT CAGCAGAGCG ATAAGTTGGA ACACTTTAGT 
CGACAGATTG AATACTTCCG CTCTCTTCTA GATGAGCATC ACATTTCTTA TGTCATAGAT 
GAAGATGTAA AAAGTGGGCG CTATATGGAA TTAGAGCAAC GTTACATGGA CCTCGCTGAG 
AATGCCCGTT TTGAACGGGA GCAGCTTCTT GGTGTCCAGC AGCATTTAAG CAATACTTTG 
AAAATGGCAG AACAAGACAA TAAGGAAGCT CAAGAAATGA TAGGGGCACT CAAAGAACGC 
AGTCACCATA TGGAGCGAAT TATTGAGTCT GAGCAGAAAG GAAAAGCAGC CTTGGCAGCC 
ACGTTAGAGG AATACAAAGC CACAGTGGCC AGTGACCAGA TAGAGATGAA TCGCCTGAAG 
GCTCAGCTGG AGAATGAAAA GCAGAAAGTG GCAGAGCTGT ATTCTATCCA TAACTCTGGA 
GACAAATCTG ATATTCAGGA CCTCCTGGAG AGTGTCAGGC TGGACAAAGA AAAAGCAGAG 
ACTTTGGCTA GTAGCTTGCA GGAAGATCTG GCTCATACCC GAAATGATGC CAATCGATTA 
CAGGATGCCA TTGCTAAGGT AGAGGATGAA TACCGAGCCT TCCAAGAAGA AGCTAAGAAA 
CAAATTGAAG ATTTGAATAT GACGTTAGAA AAATTAAGAT CAGACCTGGA TGAAAAAGAA 
ACAGAAAGGA GTGACATGAA AGAAACCATC TTTGAACTTG AAGATGAAGT AGAACAACAT 
CGTGCTGTGA AACTTCATGA CAACCTCATT ATTTCTGATC TAGAGAATAC AGTTAAAAAA 
CTCCAGGACC AAAAGCACGA CATGGAAAGA GAAATAAAGA CACTCCACAG AAGACTTCGG 
GAAGAATCTG CGGAATGGCG GCAGTTTCAG GCTGATCTCC AGACTGCAGT AGTCATTGCA 
AATGACATTA AATCTGAAGC CCAAGAGGAG ATTGGTGATC TAAAGCGCCG GTTACATGAG 
GCTCAAGAAA AAAATGAGAA ACTCACAAAA GAATTGGAGG AAATAAAGTC ACGCAAGCAA 
GAGGAGGAGC GAGGCCGGGT ATACAATTAC ATGAATGCCG TTGAGAGAGA TTTGGCAGCC 
TTAAGGCAGG GAATGGGACT GAGTAGAAGG TCCTCGACTT CCTCAGAGCC AACTCCTACA 
GTAAAAACCC TCATCAAGTC CTTTGACAGT GCATCTCAAG TACCAAACCC TGCTGCAGCT 
GCAATTCCTC GAACGCCCCT GAGCCCAAGT CCTATGAAAA CCCCTCCTGC AGCAGCTGTG 
TCCCCTATGC AGAGACATTC CATAAGTGGA CCAATCTCAA CATCCAAACC CCTGACAGCC 
CTGTCAGATA AGAGACCAAA CTATGGGGAA ATCCCTGTTC AAGAGCATCT GTTAAGAACA 
TCTTCAGCCA GCCGGCCTGC TTCCCTGCCA AGAGTGCCTG CGATGGAAAG TGCCAAGACC 
CTCTCAGTGT CTCGACGAAG TAGTGAAGAA ATGAAACGGG ACATTTCTGC ACAGGAGGGA 
GCGTCGCCAG CCTCTCTGAT GGCTATGGGA ACCACGTCTC CACAGCTTTC CCTGTCCTCT 
TCTCCAACGG CATCTGTGAC TCCCACCACC CGAAGCCGAA TAAGAGAAGA AAGGAAAGAC 
CCTCTCTCAG CATTGGCCAG AGAATATGGA GGATCAAAGA GGAACGCCTT GCTGAAGTGG 
TGTCAGAAGA AAACAGAAGG CTATCAGAAT ATTGACATTA CAAACTTCAG CAGCAGCTGG 
AATGATGGGC TGGCCTTCTG TGCCCTCCTG CATACATATC TCCCTGCCCA CATTCCATAT 
CAAGAACTGA ACAGCCAGGA TAAGAGAAGG AACTTCATGC TGGCTTTCCA GGCAGCTGAA 
AGTGTCGGCA TCAAATCCAC ACTGGACATT AATGAAATGG TACGGACTGA ACGACCCGAC 
TGGCAGAACG TGATGCTGTA TGTGACGGCG ATCTACAAGT ACTTTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPECC1L ( NM_015330.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_056145.4 Homo sapiens sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00640
Clone ID Related Accession (Same CDS sequence)	NM_001145468.3 , NM_015330.4 , NM_015330.5
Accession Version	NM_015330.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	cytospin-A isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY105216.1, AL832425.1, BC013085.1, BC021132.1 and BM126792.1. On Nov 22, 2018 this sequence version replaced NM_015330.4. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660803.23307.1, AL832425.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAGAAAG CAAGCAGGAG TGTTGGCTCA GTGCCTAAAG TGTCTGCAAT AAGTAAAACG 
CAAACAGCAG AAAAAATTAA ACCTGAAAAC AGCTCTTCAG CATCTACGGG AGGCAAACTT 
GTAAAACCTG GAACAGCAGC ATCATTGTCA AAGACCAAGA GCAGTGATGA CCTTTTAGCT 
GGAATGGCCG GAGGGGTAAC GGTGACTAAT GGTGTTAAAG GAAAGAAAAG CACCTGCCCA 
TCTGCAGCAC CTTCAGCATC TGCCCCTGCC ATGACCACCG TGGAGAACAA ATCCAAGATT 
AGCACAGGCA CAGCTTCTTC AACCAAGCGG AGCACTTCTA CAGGTAATAA AGAATCCAGT 
TCTACTAGAG AAAGATTACG TGAACGTACC CGATTAAACC AGAGCAAAAA ACTACCTTCT 
GCAGGTCAGG GAGCTAATGA CATGGCATTG GCCAAACGTT CCCGCAGTCG AACTGCTACA 
GAATGTGACG TTCGTATGAG CAAGTCTAAG TCAGACAATC AGATCAGTGA CAGAGCTGCT 
TTGGAGGCCA AAGTGAAGGA TCTTCTCACG CTGGCAAAAA CCAAAGACGT AGAAATTTTA 
CATTTGAGAA ATGAACTGCG AGACATGCGT GCCCAGCTGG GCATTAATGA GGATCATTCT 
GAGGGTGATG AAAAATCTGA GAAGGAAACT ATTATGGCTC ACCAGCCGAC TGATGTGGAG 
TCCACTTTAT TGCAGTTGCA GGAACAGAAT ACTGCCATCC GTGAAGAACT CAACCAGCTG 
AAAAATGAAA ACAGAATGTT AAAGGACAGG TTGAATGCAT TGGGCTTTTC CCTAGAGCAG 
AGGTTAGACA ATTCTGAAAA ACTGTTTGGC TATCAGTCCC TGAGCCCAGA AATCACCCCT 
GGTAACCAGA GCGATGGAGG AGGAACTCTG ACTTCTTCAG TGGAAGGCTC TGCCCCTGGC 
TCAGTGGAGG ATCTCTTGAG TCAGGATGAA AATACACTAA TGGACCATCA GCACAGTAAC 
TCCATGGACA ATTTAGACAG TGAGTGCAGT GAGGTCTACC AGCCCCTCAC ATCGAGCGAT 
GATGCGCTGG ATGCACCATC CTCCTCAGAG TCGGAAGGCA TCCCCAGCAT AGAGCGCTCC 
CGGAAGGGGA GCAGCGGGAA TGCCAGTGAA GTGTCCGTGG CTTGCCTGAC TGAACGGATA 
CACCAGATGG AAGAGAACCA ACACAGTACA AGTGAGGAAC TCCAGGCAAC CCTGCAAGAG 
CTAGCTGATT TACAGCAGAT TACCCAGGAA CTGAATAGTG AAAACGAAAG GCTTGGAGAA 
GAGAAGGTTA TTCTGATGGA GTCTTTATGT CAGCAGAGCG ATAAGTTGGA ACACTTTAGT 
CGACAGATTG AATACTTCCG CTCTCTTCTA GATGAGCATC ACATTTCTTA TGTCATAGAT 
GAAGATGTAA AAAGTGGGCG CTATATGGAA TTAGAGCAAC GTTACATGGA CCTCGCTGAG 
AATGCCCGTT TTGAACGGGA GCAGCTTCTT GGTGTCCAGC AGCATTTAAG CAATACTTTG 
AAAATGGCAG AACAAGACAA TAAGGAAGCT CAAGAAATGA TAGGGGCACT CAAAGAACGC 
AGTCACCATA TGGAGCGAAT TATTGAGTCT GAGCAGAAAG GAAAAGCAGC CTTGGCAGCC 
ACGTTAGAGG AATACAAAGC CACAGTGGCC AGTGACCAGA TAGAGATGAA TCGCCTGAAG 
GCTCAGCTGG AGAATGAAAA GCAGAAAGTG GCAGAGCTGT ATTCTATCCA TAACTCTGGA 
GACAAATCTG ATATTCAGGA CCTCCTGGAG AGTGTCAGGC TGGACAAAGA AAAAGCAGAG 
ACTTTGGCTA GTAGCTTGCA GGAAGATCTG GCTCATACCC GAAATGATGC CAATCGATTA 
CAGGATGCCA TTGCTAAGGT AGAGGATGAA TACCGAGCCT TCCAAGAAGA AGCTAAGAAA 
CAAATTGAAG ATTTGAATAT GACGTTAGAA AAATTAAGAT CAGACCTGGA TGAAAAAGAA 
ACAGAAAGGA GTGACATGAA AGAAACCATC TTTGAACTTG AAGATGAAGT AGAACAACAT 
CGTGCTGTGA AACTTCATGA CAACCTCATT ATTTCTGATC TAGAGAATAC AGTTAAAAAA 
CTCCAGGACC AAAAGCACGA CATGGAAAGA GAAATAAAGA CACTCCACAG AAGACTTCGG 
GAAGAATCTG CGGAATGGCG GCAGTTTCAG GCTGATCTCC AGACTGCAGT AGTCATTGCA 
AATGACATTA AATCTGAAGC CCAAGAGGAG ATTGGTGATC TAAAGCGCCG GTTACATGAG 
GCTCAAGAAA AAAATGAGAA ACTCACAAAA GAATTGGAGG AAATAAAGTC ACGCAAGCAA 
GAGGAGGAGC GAGGCCGGGT ATACAATTAC ATGAATGCCG TTGAGAGAGA TTTGGCAGCC 
TTAAGGCAGG GAATGGGACT GAGTAGAAGG TCCTCGACTT CCTCAGAGCC AACTCCTACA 
GTAAAAACCC TCATCAAGTC CTTTGACAGT GCATCTCAAG TACCAAACCC TGCTGCAGCT 
GCAATTCCTC GAACGCCCCT GAGCCCAAGT CCTATGAAAA CCCCTCCTGC AGCAGCTGTG 
TCCCCTATGC AGAGACATTC CATAAGTGGA CCAATCTCAA CATCCAAACC CCTGACAGCC 
CTGTCAGATA AGAGACCAAA CTATGGGGAA ATCCCTGTTC AAGAGCATCT GTTAAGAACA 
TCTTCAGCCA GCCGGCCTGC TTCCCTGCCA AGAGTGCCTG CGATGGAAAG TGCCAAGACC 
CTCTCAGTGT CTCGACGAAG TAGTGAAGAA ATGAAACGGG ACATTTCTGC ACAGGAGGGA 
GCGTCGCCAG CCTCTCTGAT GGCTATGGGA ACCACGTCTC CACAGCTTTC CCTGTCCTCT 
TCTCCAACGG CATCTGTGAC TCCCACCACC CGAAGCCGAA TAAGAGAAGA AAGGAAAGAC 
CCTCTCTCAG CATTGGCCAG AGAATATGGA GGATCAAAGA GGAACGCCTT GCTGAAGTGG 
TGTCAGAAGA AAACAGAAGG CTATCAGAAT ATTGACATTA CAAACTTCAG CAGCAGCTGG 
AATGATGGGC TGGCCTTCTG TGCCCTCCTG CATACATATC TCCCTGCCCA CATTCCATAT 
CAAGAACTGA ACAGCCAGGA TAAGAGAAGG AACTTCATGC TGGCTTTCCA GGCAGCTGAA 
AGTGTCGGCA TCAAATCCAC ACTGGACATT AATGAAATGG TACGGACTGA ACGACCCGAC 
TGGCAGAACG TGATGCTGTA TGTGACGGCG ATCTACAAGT ACTTTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056145.4
CDS	295..3648
Translation	MKKASRSVGSVPKVSAISKTQTAEKIKPENSSSASTGGKLVKPGTAASLSKTKSSDDLLAGMAGGVTVTNGVKGKKSTCPSAAPSASAPAMTTVENKSKISTGTASSTKRSTSTGNKESSSTRERLRERTRLNQSKKLPSAGQGANDMALAKRSRSRTATECDVRMSKSKSDNQISDRAALEAKVKDLLTLAKTKDVEILHLRNELRDMRAQLGINEDHSEGDEKSEKETIMAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLEQRLDNSEKLFGYQSLSPEITPGNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSECSEVYQPLTSSDDALDAPSSSESEGIPSIERSRKGSSGNASEVSVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNSENERLGEEKVILMESLCQQSDKLEHFSRQIEYFRSLLDEHHISYVIDEDVKSGRYMELEQRYMDLAENARFEREQLLGVQQHLSNTLKMAEQDNKEAQEMIGALKERSHHMERIIESEQKGKAALAATLEEYKATVASDQIEMNRLKAQLENEKQKVAELYSIHNSGDKSDIQDLLESVRLDKEKAETLASSLQEDLAHTRNDANRLQDAIAKVEDEYRAFQEEAKKQIEDLNMTLEKLRSDLDEKETERSDMKETIFELEDEVEQHRAVKLHDNLIISDLENTVKKLQDQKHDMEREIKTLHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLKRRLHEAQEKNEKLTKELEEIKSRKQEEERGRVYNYMNAVERDLAALRQGMGLSRRSSTSSEPTPTVKTLIKSFDSASQVPNPAAAAIPRTPLSPSPMKTPPAAAVSPMQRHSISGPISTSKPLTALSDKRPNYGEIPVQEHLLRTSSASRPASLPRVPAMESAKTLSVSRRSSEEMKRDISAQEGASPASLMAMGTTSPQLSLSSSPTASVTPTTRSRIREERKDPLSALAREYGGSKRNALLKWCQKKTEGYQNIDITNFSSSWNDGLAFCALLHTYLPAHIPYQELNSQDKRRNFMLAFQAAESVGIKSTLDINEMVRTERPDWQNVMLYVTAIYKYFET

Target ORF information:

RefSeq Version	NM_015330.5
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015330.5

ORF Insert Sequence:

ATGAAGAAAG CAAGCAGGAG TGTTGGCTCA GTGCCTAAAG TGTCTGCAAT AAGTAAAACG 
CAAACAGCAG AAAAAATTAA ACCTGAAAAC AGCTCTTCAG CATCTACGGG AGGCAAACTT 
GTAAAACCTG GAACAGCAGC ATCATTGTCA AAGACCAAGA GCAGTGATGA CCTTTTAGCT 
GGAATGGCCG GAGGGGTAAC GGTGACTAAT GGTGTTAAAG GAAAGAAAAG CACCTGCCCA 
TCTGCAGCAC CTTCAGCATC TGCCCCTGCC ATGACCACCG TGGAGAACAA ATCCAAGATT 
AGCACAGGCA CAGCTTCTTC AACCAAGCGG AGCACTTCTA CAGGTAATAA AGAATCCAGT 
TCTACTAGAG AAAGATTACG TGAACGTACC CGATTAAACC AGAGCAAAAA ACTACCTTCT 
GCAGGTCAGG GAGCTAATGA CATGGCATTG GCCAAACGTT CCCGCAGTCG AACTGCTACA 
GAATGTGACG TTCGTATGAG CAAGTCTAAG TCAGACAATC AGATCAGTGA CAGAGCTGCT 
TTGGAGGCCA AAGTGAAGGA TCTTCTCACG CTGGCAAAAA CCAAAGACGT AGAAATTTTA 
CATTTGAGAA ATGAACTGCG AGACATGCGT GCCCAGCTGG GCATTAATGA GGATCATTCT 
GAGGGTGATG AAAAATCTGA GAAGGAAACT ATTATGGCTC ACCAGCCGAC TGATGTGGAG 
TCCACTTTAT TGCAGTTGCA GGAACAGAAT ACTGCCATCC GTGAAGAACT CAACCAGCTG 
AAAAATGAAA ACAGAATGTT AAAGGACAGG TTGAATGCAT TGGGCTTTTC CCTAGAGCAG 
AGGTTAGACA ATTCTGAAAA ACTGTTTGGC TATCAGTCCC TGAGCCCAGA AATCACCCCT 
GGTAACCAGA GCGATGGAGG AGGAACTCTG ACTTCTTCAG TGGAAGGCTC TGCCCCTGGC 
TCAGTGGAGG ATCTCTTGAG TCAGGATGAA AATACACTAA TGGACCATCA GCACAGTAAC 
TCCATGGACA ATTTAGACAG TGAGTGCAGT GAGGTCTACC AGCCCCTCAC ATCGAGCGAT 
GATGCGCTGG ATGCACCATC CTCCTCAGAG TCGGAAGGCA TCCCCAGCAT AGAGCGCTCC 
CGGAAGGGGA GCAGCGGGAA TGCCAGTGAA GTGTCCGTGG CTTGCCTGAC TGAACGGATA 
CACCAGATGG AAGAGAACCA ACACAGTACA AGTGAGGAAC TCCAGGCAAC CCTGCAAGAG 
CTAGCTGATT TACAGCAGAT TACCCAGGAA CTGAATAGTG AAAACGAAAG GCTTGGAGAA 
GAGAAGGTTA TTCTGATGGA GTCTTTATGT CAGCAGAGCG ATAAGTTGGA ACACTTTAGT 
CGACAGATTG AATACTTCCG CTCTCTTCTA GATGAGCATC ACATTTCTTA TGTCATAGAT 
GAAGATGTAA AAAGTGGGCG CTATATGGAA TTAGAGCAAC GTTACATGGA CCTCGCTGAG 
AATGCCCGTT TTGAACGGGA GCAGCTTCTT GGTGTCCAGC AGCATTTAAG CAATACTTTG 
AAAATGGCAG AACAAGACAA TAAGGAAGCT CAAGAAATGA TAGGGGCACT CAAAGAACGC 
AGTCACCATA TGGAGCGAAT TATTGAGTCT GAGCAGAAAG GAAAAGCAGC CTTGGCAGCC 
ACGTTAGAGG AATACAAAGC CACAGTGGCC AGTGACCAGA TAGAGATGAA TCGCCTGAAG 
GCTCAGCTGG AGAATGAAAA GCAGAAAGTG GCAGAGCTGT ATTCTATCCA TAACTCTGGA 
GACAAATCTG ATATTCAGGA CCTCCTGGAG AGTGTCAGGC TGGACAAAGA AAAAGCAGAG 
ACTTTGGCTA GTAGCTTGCA GGAAGATCTG GCTCATACCC GAAATGATGC CAATCGATTA 
CAGGATGCCA TTGCTAAGGT AGAGGATGAA TACCGAGCCT TCCAAGAAGA AGCTAAGAAA 
CAAATTGAAG ATTTGAATAT GACGTTAGAA AAATTAAGAT CAGACCTGGA TGAAAAAGAA 
ACAGAAAGGA GTGACATGAA AGAAACCATC TTTGAACTTG AAGATGAAGT AGAACAACAT 
CGTGCTGTGA AACTTCATGA CAACCTCATT ATTTCTGATC TAGAGAATAC AGTTAAAAAA 
CTCCAGGACC AAAAGCACGA CATGGAAAGA GAAATAAAGA CACTCCACAG AAGACTTCGG 
GAAGAATCTG CGGAATGGCG GCAGTTTCAG GCTGATCTCC AGACTGCAGT AGTCATTGCA 
AATGACATTA AATCTGAAGC CCAAGAGGAG ATTGGTGATC TAAAGCGCCG GTTACATGAG 
GCTCAAGAAA AAAATGAGAA ACTCACAAAA GAATTGGAGG AAATAAAGTC ACGCAAGCAA 
GAGGAGGAGC GAGGCCGGGT ATACAATTAC ATGAATGCCG TTGAGAGAGA TTTGGCAGCC 
TTAAGGCAGG GAATGGGACT GAGTAGAAGG TCCTCGACTT CCTCAGAGCC AACTCCTACA 
GTAAAAACCC TCATCAAGTC CTTTGACAGT GCATCTCAAG TACCAAACCC TGCTGCAGCT 
GCAATTCCTC GAACGCCCCT GAGCCCAAGT CCTATGAAAA CCCCTCCTGC AGCAGCTGTG 
TCCCCTATGC AGAGACATTC CATAAGTGGA CCAATCTCAA CATCCAAACC CCTGACAGCC 
CTGTCAGATA AGAGACCAAA CTATGGGGAA ATCCCTGTTC AAGAGCATCT GTTAAGAACA 
TCTTCAGCCA GCCGGCCTGC TTCCCTGCCA AGAGTGCCTG CGATGGAAAG TGCCAAGACC 
CTCTCAGTGT CTCGACGAAG TAGTGAAGAA ATGAAACGGG ACATTTCTGC ACAGGAGGGA 
GCGTCGCCAG CCTCTCTGAT GGCTATGGGA ACCACGTCTC CACAGCTTTC CCTGTCCTCT 
TCTCCAACGG CATCTGTGAC TCCCACCACC CGAAGCCGAA TAAGAGAAGA AAGGAAAGAC 
CCTCTCTCAG CATTGGCCAG AGAATATGGA GGATCAAAGA GGAACGCCTT GCTGAAGTGG 
TGTCAGAAGA AAACAGAAGG CTATCAGAAT ATTGACATTA CAAACTTCAG CAGCAGCTGG 
AATGATGGGC TGGCCTTCTG TGCCCTCCTG CATACATATC TCCCTGCCCA CATTCCATAT 
CAAGAACTGA ACAGCCAGGA TAAGAGAAGG AACTTCATGC TGGCTTTCCA GGCAGCTGAA 
AGTGTCGGCA TCAAATCCAC ACTGGACATT AATGAAATGG TACGGACTGA ACGACCCGAC 
TGGCAGAACG TGATGCTGTA TGTGACGGCG ATCTACAAGT ACTTTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPECC1L ( NM_001254732.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001241661.2 Homo sapiens sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant 3, mRNA.

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CloneID	OHu02602
Clone ID Related Accession (Same CDS sequence)	NM_001254732.2
Accession Version	NM_001254732.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	cytospin-A isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY105216.1, AK301482.1, BM556934.1, BM720120.1, BC021132.1, BM126792.1 and AA773104.1. On Jun 20, 2013 this sequence version replaced NM_001254732.1. Transcript Variant: This variant (3) differs in the 5' UTR and lacks an exon in the 3' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK301482.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGAAAG CAAGCAGGAG TGTTGGCTCA GTGCCTAAAG TGTCTGCAAT AAGTAAAACG 
CAAACAGCAG AAAAAATTAA ACCTGAAAAC AGCTCTTCAG CATCTACGGG AGGCAAACTT 
GTAAAACCTG GAACAGCAGC ATCATTGTCA AAGACCAAGA GCAGTGATGA CCTTTTAGCT 
GGAATGGCCG GAGGGGTAAC GGTGACTAAT GGTGTTAAAG GAAAGAAAAG CACCTGCCCA 
TCTGCAGCAC CTTCAGCATC TGCCCCTGCC ATGACCACCG TGGAGAACAA ATCCAAGATT 
AGCACAGGCA CAGCTTCTTC AACCAAGCGG AGCACTTCTA CAGGTAATAA AGAATCCAGT 
TCTACTAGAG AAAGATTACG TGAACGTACC CGATTAAACC AGAGCAAAAA ACTACCTTCT 
GCAGGTCAGG GAGCTAATGA CATGGCATTG GCCAAACGTT CCCGCAGTCG AACTGCTACA 
GAATGTGACG TTCGTATGAG CAAGTCTAAG TCAGACAATC AGATCAGTGA CAGAGCTGCT 
TTGGAGGCCA AAGTGAAGGA TCTTCTCACG CTGGCAAAAA CCAAAGACGT AGAAATTTTA 
CATTTGAGAA ATGAACTGCG AGACATGCGT GCCCAGCTGG GCATTAATGA GGATCATTCT 
GAGGGTGATG AAAAATCTGA GAAGGAAACT ATTATGGCTC ACCAGCCGAC TGATGTGGAG 
TCCACTTTAT TGCAGTTGCA GGAACAGAAT ACTGCCATCC GTGAAGAACT CAACCAGCTG 
AAAAATGAAA ACAGAATGTT AAAGGACAGG TTGAATGCAT TGGGCTTTTC CCTAGAGCAG 
AGGTTAGACA ATTCTGAAAA ACTGTTTGGC TATCAGTCCC TGAGCCCAGA AATCACCCCT 
GGTAACCAGA GCGATGGAGG AGGAACTCTG ACTTCTTCAG TGGAAGGCTC TGCCCCTGGC 
TCAGTGGAGG ATCTCTTGAG TCAGGATGAA AATACACTAA TGGACCATCA GCACAGTAAC 
TCCATGGACA ATTTAGACAG TGAGTGCAGT GAGGTCTACC AGCCCCTCAC ATCGAGCGAT 
GATGCGCTGG ATGCACCATC CTCCTCAGAG TCGGAAGGCA TCCCCAGCAT AGAGCGCTCC 
CGGAAGGGGA GCAGCGGGAA TGCCAGTGAA GTGTCCGTGG CTTGCCTGAC TGAACGGATA 
CACCAGATGG AAGAGAACCA ACACAGTACA AGTGAGGAAC TCCAGGCAAC CCTGCAAGAG 
CTAGCTGATT TACAGCAGAT TACCCAGGAA CTGAATAGTG AAAACGAAAG GCTTGGAGAA 
GAGAAGGTTA TTCTGATGGA GTCTTTATGT CAGCAGAGCG ATAAGTTGGA ACACTTTAGT 
CGACAGATTG AATACTTCCG CTCTCTTCTA GATGAGCATC ACATTTCTTA TGTCATAGAT 
GAAGATGTAA AAAGTGGGCG CTATATGGAA TTAGAGCAAC GTTACATGGA CCTCGCTGAG 
AATGCCCGTT TTGAACGGGA GCAGCTTCTT GGTGTCCAGC AGCATTTAAG CAATACTTTG 
AAAATGGCAG AACAAGACAA TAAGGAAGCT CAAGAAATGA TAGGGGCACT CAAAGAACGC 
AGTCACCATA TGGAGCGAAT TATTGAGTCT GAGCAGAAAG GAAAAGCAGC CTTGGCAGCC 
ACGTTAGAGG AATACAAAGC CACAGTGGCC AGTGACCAGA TAGAGATGAA TCGCCTGAAG 
GCTCAGCTGG AGAATGAAAA GCAGAAAGTG GCAGAGCTGT ATTCTATCCA TAACTCTGGA 
GACAAATCTG ATATTCAGGA CCTCCTGGAG AGTGTCAGGC TGGACAAAGA AAAAGCAGAG 
ACTTTGGCTA GTAGCTTGCA GGAAGATCTG GCTCATACCC GAAATGATGC CAATCGATTA 
CAGGATGCCA TTGCTAAGGT AGAGGATGAA TACCGAGCCT TCCAAGAAGA AGCTAAGAAA 
CAAATTGAAG ATTTGAATAT GACGTTAGAA AAATTAAGAT CAGACCTGGA TGAAAAAGAA 
ACAGAAAGGA GTGACATGAA AGAAACCATC TTTGAACTTG AAGATGAAGT AGAACAACAT 
CGTGCTGTGA AACTTCATGA CAACCTCATT ATTTCTGATC TAGAGAATAC AGTTAAAAAA 
CTCCAGGACC AAAAGCACGA CATGGAAAGA GAAATAAAGA CACTCCACAG AAGACTTCGG 
GAAGAATCTG CGGAATGGCG GCAGTTTCAG GCTGATCTCC AGACTGCAGT AGTCATTGCA 
AATGACATTA AATCTGAAGC CCAAGAGGAG ATTGGTGATC TAAAGCGCCG GTTACATGAG 
GCTCAAGAAA AAAATGAGAA ACTCACAAAA GAATTGGAGG AAATAAAGTC ACGCAAGCAA 
GAGGAGGAGC GAGGCCGGGT ATACAATTAC ATGAATGCCG TTGAGAGAGA TTTGGCAGCC 
TTAAGGCAGG GAATGGGACT GAGTAGAAGG TCCTCGACTT CCTCAGAGCC AACTCCTACA 
GTAAAAACCC TCATCAAGTC CTTTGACAGT GCATCTCAAG TACCAAACCC TGCTGCAGCT 
GCAATTCCTC GAACGCCCCT GAGCCCAAGT CCTATGAAAA CCCCTCCTGC AGCAGCTGTG 
TCCCCTATGC AGAGACATTC CATAAGTGGA CCAATCTCAA CATCCAAACC CCTGACAGCC 
CTGTCAGATA AGAGACCAAA CTATGGGGAA ATCCCTGTTC AAGAGCATCT GTTAAGAACA 
TCTTCAGCCA GCCGGCCTGC TTCCCTGCCA AGAGTGCCTG CGATGGAAAG TGCCAAGACC 
CTCTCAGTGT CTCGACGAAG TAGTGAAGAA ATGAAACGGG ACATTTCTGC ACAGGAGGGA 
GCGTCGCCAG CCTCTCTGAT GGCTATGGGA ACCACGTCTC CACAGCTTTC CCTGTCCTCT 
TCTCCAACGG CATCTGTGAC TCCCACCACC CGAAGCCGAA TAAGAGAAGA AAGGAAAGAC 
CCTCTCTCAG CATTGGCCAG AGAATATGGA GGATCAAAGA GGAACGCCTT GCTGAAGTGG 
TGTCAGAAGA AAACAGAAGG CTATCAGAGA AGGAACTTCA TGCTGGCTTT CCAGGCAGCT 
GAAAGTGTCG GCATCAAATC CACACTGGAC ATTAATGAAA TGGTACGGAC TGAACGACCC 
GACTGGCAGA ACGTGATGCT GTATGTGACG GCGATCTACA AGTACTTTGA GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001241661.2
CDS	205..3441
Translation	MKKASRSVGSVPKVSAISKTQTAEKIKPENSSSASTGGKLVKPGTAASLSKTKSSDDLLAGMAGGVTVTNGVKGKKSTCPSAAPSASAPAMTTVENKSKISTGTASSTKRSTSTGNKESSSTRERLRERTRLNQSKKLPSAGQGANDMALAKRSRSRTATECDVRMSKSKSDNQISDRAALEAKVKDLLTLAKTKDVEILHLRNELRDMRAQLGINEDHSEGDEKSEKETIMAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLEQRLDNSEKLFGYQSLSPEITPGNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSECSEVYQPLTSSDDALDAPSSSESEGIPSIERSRKGSSGNASEVSVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNSENERLGEEKVILMESLCQQSDKLEHFSRQIEYFRSLLDEHHISYVIDEDVKSGRYMELEQRYMDLAENARFEREQLLGVQQHLSNTLKMAEQDNKEAQEMIGALKERSHHMERIIESEQKGKAALAATLEEYKATVASDQIEMNRLKAQLENEKQKVAELYSIHNSGDKSDIQDLLESVRLDKEKAETLASSLQEDLAHTRNDANRLQDAIAKVEDEYRAFQEEAKKQIEDLNMTLEKLRSDLDEKETERSDMKETIFELEDEVEQHRAVKLHDNLIISDLENTVKKLQDQKHDMEREIKTLHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLKRRLHEAQEKNEKLTKELEEIKSRKQEEERGRVYNYMNAVERDLAALRQGMGLSRRSSTSSEPTPTVKTLIKSFDSASQVPNPAAAAIPRTPLSPSPMKTPPAAAVSPMQRHSISGPISTSKPLTALSDKRPNYGEIPVQEHLLRTSSASRPASLPRVPAMESAKTLSVSRRSSEEMKRDISAQEGASPASLMAMGTTSPQLSLSSSPTASVTPTTRSRIREERKDPLSALAREYGGSKRNALLKWCQKKTEGYQRRNFMLAFQAAESVGIKSTLDINEMVRTERPDWQNVMLYVTAIYKYFET

Target ORF information:

RefSeq Version	NM_001254732.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001254732.2

ORF Insert Sequence:

ATGAAGAAAG CAAGCAGGAG TGTTGGCTCA GTGCCTAAAG TGTCTGCAAT AAGTAAAACG 
CAAACAGCAG AAAAAATTAA ACCTGAAAAC AGCTCTTCAG CATCTACGGG AGGCAAACTT 
GTAAAACCTG GAACAGCAGC ATCATTGTCA AAGACCAAGA GCAGTGATGA CCTTTTAGCT 
GGAATGGCCG GAGGGGTAAC GGTGACTAAT GGTGTTAAAG GAAAGAAAAG CACCTGCCCA 
TCTGCAGCAC CTTCAGCATC TGCCCCTGCC ATGACCACCG TGGAGAACAA ATCCAAGATT 
AGCACAGGCA CAGCTTCTTC AACCAAGCGG AGCACTTCTA CAGGTAATAA AGAATCCAGT 
TCTACTAGAG AAAGATTACG TGAACGTACC CGATTAAACC AGAGCAAAAA ACTACCTTCT 
GCAGGTCAGG GAGCTAATGA CATGGCATTG GCCAAACGTT CCCGCAGTCG AACTGCTACA 
GAATGTGACG TTCGTATGAG CAAGTCTAAG TCAGACAATC AGATCAGTGA CAGAGCTGCT 
TTGGAGGCCA AAGTGAAGGA TCTTCTCACG CTGGCAAAAA CCAAAGACGT AGAAATTTTA 
CATTTGAGAA ATGAACTGCG AGACATGCGT GCCCAGCTGG GCATTAATGA GGATCATTCT 
GAGGGTGATG AAAAATCTGA GAAGGAAACT ATTATGGCTC ACCAGCCGAC TGATGTGGAG 
TCCACTTTAT TGCAGTTGCA GGAACAGAAT ACTGCCATCC GTGAAGAACT CAACCAGCTG 
AAAAATGAAA ACAGAATGTT AAAGGACAGG TTGAATGCAT TGGGCTTTTC CCTAGAGCAG 
AGGTTAGACA ATTCTGAAAA ACTGTTTGGC TATCAGTCCC TGAGCCCAGA AATCACCCCT 
GGTAACCAGA GCGATGGAGG AGGAACTCTG ACTTCTTCAG TGGAAGGCTC TGCCCCTGGC 
TCAGTGGAGG ATCTCTTGAG TCAGGATGAA AATACACTAA TGGACCATCA GCACAGTAAC 
TCCATGGACA ATTTAGACAG TGAGTGCAGT GAGGTCTACC AGCCCCTCAC ATCGAGCGAT 
GATGCGCTGG ATGCACCATC CTCCTCAGAG TCGGAAGGCA TCCCCAGCAT AGAGCGCTCC 
CGGAAGGGGA GCAGCGGGAA TGCCAGTGAA GTGTCCGTGG CTTGCCTGAC TGAACGGATA 
CACCAGATGG AAGAGAACCA ACACAGTACA AGTGAGGAAC TCCAGGCAAC CCTGCAAGAG 
CTAGCTGATT TACAGCAGAT TACCCAGGAA CTGAATAGTG AAAACGAAAG GCTTGGAGAA 
GAGAAGGTTA TTCTGATGGA GTCTTTATGT CAGCAGAGCG ATAAGTTGGA ACACTTTAGT 
CGACAGATTG AATACTTCCG CTCTCTTCTA GATGAGCATC ACATTTCTTA TGTCATAGAT 
GAAGATGTAA AAAGTGGGCG CTATATGGAA TTAGAGCAAC GTTACATGGA CCTCGCTGAG 
AATGCCCGTT TTGAACGGGA GCAGCTTCTT GGTGTCCAGC AGCATTTAAG CAATACTTTG 
AAAATGGCAG AACAAGACAA TAAGGAAGCT CAAGAAATGA TAGGGGCACT CAAAGAACGC 
AGTCACCATA TGGAGCGAAT TATTGAGTCT GAGCAGAAAG GAAAAGCAGC CTTGGCAGCC 
ACGTTAGAGG AATACAAAGC CACAGTGGCC AGTGACCAGA TAGAGATGAA TCGCCTGAAG 
GCTCAGCTGG AGAATGAAAA GCAGAAAGTG GCAGAGCTGT ATTCTATCCA TAACTCTGGA 
GACAAATCTG ATATTCAGGA CCTCCTGGAG AGTGTCAGGC TGGACAAAGA AAAAGCAGAG 
ACTTTGGCTA GTAGCTTGCA GGAAGATCTG GCTCATACCC GAAATGATGC CAATCGATTA 
CAGGATGCCA TTGCTAAGGT AGAGGATGAA TACCGAGCCT TCCAAGAAGA AGCTAAGAAA 
CAAATTGAAG ATTTGAATAT GACGTTAGAA AAATTAAGAT CAGACCTGGA TGAAAAAGAA 
ACAGAAAGGA GTGACATGAA AGAAACCATC TTTGAACTTG AAGATGAAGT AGAACAACAT 
CGTGCTGTGA AACTTCATGA CAACCTCATT ATTTCTGATC TAGAGAATAC AGTTAAAAAA 
CTCCAGGACC AAAAGCACGA CATGGAAAGA GAAATAAAGA CACTCCACAG AAGACTTCGG 
GAAGAATCTG CGGAATGGCG GCAGTTTCAG GCTGATCTCC AGACTGCAGT AGTCATTGCA 
AATGACATTA AATCTGAAGC CCAAGAGGAG ATTGGTGATC TAAAGCGCCG GTTACATGAG 
GCTCAAGAAA AAAATGAGAA ACTCACAAAA GAATTGGAGG AAATAAAGTC ACGCAAGCAA 
GAGGAGGAGC GAGGCCGGGT ATACAATTAC ATGAATGCCG TTGAGAGAGA TTTGGCAGCC 
TTAAGGCAGG GAATGGGACT GAGTAGAAGG TCCTCGACTT CCTCAGAGCC AACTCCTACA 
GTAAAAACCC TCATCAAGTC CTTTGACAGT GCATCTCAAG TACCAAACCC TGCTGCAGCT 
GCAATTCCTC GAACGCCCCT GAGCCCAAGT CCTATGAAAA CCCCTCCTGC AGCAGCTGTG 
TCCCCTATGC AGAGACATTC CATAAGTGGA CCAATCTCAA CATCCAAACC CCTGACAGCC 
CTGTCAGATA AGAGACCAAA CTATGGGGAA ATCCCTGTTC AAGAGCATCT GTTAAGAACA 
TCTTCAGCCA GCCGGCCTGC TTCCCTGCCA AGAGTGCCTG CGATGGAAAG TGCCAAGACC 
CTCTCAGTGT CTCGACGAAG TAGTGAAGAA ATGAAACGGG ACATTTCTGC ACAGGAGGGA 
GCGTCGCCAG CCTCTCTGAT GGCTATGGGA ACCACGTCTC CACAGCTTTC CCTGTCCTCT 
TCTCCAACGG CATCTGTGAC TCCCACCACC CGAAGCCGAA TAAGAGAAGA AAGGAAAGAC 
CCTCTCTCAG CATTGGCCAG AGAATATGGA GGATCAAAGA GGAACGCCTT GCTGAAGTGG 
TGTCAGAAGA AAACAGAAGG CTATCAGAGA AGGAACTTCA TGCTGGCTTT CCAGGCAGCT 
GAAAGTGTCG GCATCAAATC CACACTGGAC ATTAATGAAA TGGTACGGAC TGAACGACCC 
GACTGGCAGA ACGTGATGCT GTATGTGACG GCGATCTACA AGTACTTTGA GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SPECC1L deficiency results in increased adherens junction stability and reduced cranial neural crest cell delamination.
Scientific reports617735(2016 Jan)
Wilson NR,Olm-Shipman AJ,Acevedo DS,Palaniyandi K,Hall EG,Kosa E,Stumpff KM,Smith GJ,Pitstick L,Liao EC,Bjork BC,Czirok A,Saadi I

Expanding the SPECC1L mutation phenotypic spectrum to include Teebi hypertelorism syndrome.
American journal of medical genetics. Part A167A(11)2497-502(2015 Nov)
Bhoj EJ,Li D,Harr MH,Tian L,Wang T,Zhao Y,Qiu H,Kim C,Hoffman JD,Hakonarson H,Zackai EH

Mutations in SPECC1L, encoding sperm antigen with calponin homology and coiled-coil domains 1-like, are found in some cases of autosomal dominant Opitz G/BBB syndrome.
Journal of medical genetics52(2)104-10(2015 Feb)
Kruszka P,Li D,Harr MH,Wilson NR,Swarr D,McCormick EM,Chiavacci RM,Li M,Martinez AF,Hart RA,McDonald-McGinn DM,Deardorff MA,Falk MJ,Allanson JE,Hudson C,Johnson JP,Saadi I,Hakonarson H,Muenke M,Zackai EH

Functional analysis of SPECC1L in craniofacial development and oblique facial cleft pathogenesis.
Plastic and reconstructive surgery134(4)748-59(2014 Oct)
Gfrerer L,Shubinets V,Hoyos T,Kong Y,Nguyen C,Pietschmann P,Morton CC,Maas RL,Liao EC

Genome-wide association study identifies three novel susceptibility loci for severe Acne vulgaris.
Nature communications54020(2014 Jun)
Navarini AA,Simpson MA,Weale M,Knight J,Carlavan I,Reiniche P,Burden DA,Layton A,Bataille V,Allen M,Pleass R,Pink A,Creamer D,English J,Munn S,Walton S,,Willis C,D?ret S,Voegel JJ,Spector T,Smith CH,Trembath RC,Barker JN

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SPECC1L cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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