specc1la cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	specc1la
Entrez Gene ID	561775
Full Name	sperm antigen with calponin homology and coiled-coil domains 1-like a
Synonyms	cytsaa,im:7159316,specc1l
General protein information	Preferred Names sperm antigen with calponin homology and coiled-coil domains 1-like a Names cytospin-A SPECC1-like protein cytospin A, genome duplicate a cytospin-A-A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005167177.4	XP_005167234.1	cytospin-A isoform X1
XM_009304055.2	XP_009302330.1	cytospin-A isoform X2
NM_001039816.1	NP_001034905.1	cytospin-A
XM_005167177.3	XP_005167234.1	cytospin-A isoform X1
XM_009304055.3	XP_009302330.1	cytospin-A isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SPECC1L	NP_001038102.1
Homo sapiens (human)	SPECC1L	NP_001138940.3
Pan troglodytes (chimpanzee)	SPECC1L	NP_001035052.1
Bos taurus (cattle)	SPECC1L	NP_001192793.1
Canis lupus familiaris (dog)	SPECC1L	NP_001041579.1
Mus musculus (house mouse)	Specc1l	NP_001139298.1
Xenopus tropicalis (tropical clawed frog)	specc1l	NP_001037875.1
	SPECC1L	XP_001094607.1
Rattus norvegicus (Norway rat)	Specc1l	NP_001034544.1
Danio rerio (zebrafish)	specc1la	NP_001034905.1

Gene Ontology
Bibliography

Related articles in PubMed

Functional analysis of SPECC1L in craniofacial development and oblique facial cleft pathogenesis.
Gfrerer L, Shubinets V, Hoyos T, Kong Y, Nguyen C, Pietschmann P, Morton CC, Maas RL, Liao EC
Plastic and reconstructive surgery134(4)748-59(2014 Oct)

Deficiency of the cytoskeletal protein SPECC1L leads to oblique facial clefting.
Saadi I, Alkuraya FS, Gisselbrecht SS, Goessling W, Cavallesco R, Turbe-Doan A, Petrin AL, Harris J, Siddiqui U, Grix AW Jr, Hove HD, Leboulch P, Glover TW, Morton CC, Richieri-Costa A, Murray JC, Erickson RP, Maas RL
American journal of human genetics89(1)44-55(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following specc1la gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the specc1la cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa38309	NM_001039816.1 Latest version!	Danio rerio sperm antigen with calponin homology and coiled-coil domains 1-like a (specc1la), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa14378	XM_005167177.4 Latest version!	Danio rerio sperm antigen with calponin homology and coiled-coil domains 1-like a (specc1la), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa43589	XM_009304055.3 Latest version!	Danio rerio sperm antigen with calponin homology and coiled-coil domains 1-like a (specc1la), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa14378	XM_005167177.3	Danio rerio sperm antigen with calponin homology and coiled-coil domains 1-like a (specc1la), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa43589	XM_009304055.2	Danio rerio sperm antigen with calponin homology and coiled-coil domains 1-like a (specc1la), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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Reference Sequences (Refseq)

specc1la ( NM_001039816.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001034905.1 Danio rerio sperm antigen with calponin homology and coiled-coil domains 1-like a (specc1la), mRNA.

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CloneID	ODa38309
Clone ID Related Accession (Same CDS sequence)	NM_001039816.1
Accession Version	NM_001039816.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-15
Organism	Danio rerio(zebrafish)
Product	cytospin-A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY884302.1. On Mar 17, 2006 this sequence version replaced XM_685184.1. ##Evidence-Data-START## Transcript exon combination :: AY884302.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAAGAAAG CAGGCAGGCC AGTGGGGAAT AAGGCAGCAA GTGGAGGAGG TAAAGGTGAC 
TCAGTGACGG CCGGAAACTG TGCTGGCAAA AACACTGTTA AGAGCCCTAC ATCAGCTTCT 
CTCTCCAAGG TGAAAAGCAA TGATGATCTT CTAGCTGCTA TGGCTGGTAG CAGTGCAGGC 
ACAAACAACA GTGTTGCCAA GGGCAAGAAG TCAACGTCTA CTCACTCAAC ATCTTGTGGA 
ACAAACACTA ATAACCCTGA CACCAAGACC AAAACCACCT CAGGTCCTTC TGGCAAACGG 
ACAACATCCA TGACCTCCAA GGAGTCGAAT TCCTCTCGAG AGCGTTTAAG AAACTCTAGA 
AACTCCAGCA GTAAAAAGCA GTCGTCCACT GGTGCTTCAG ATAGAGCTCA GCCCAAGCAT 
TCCCGTGCAC TGGCCCAGAC CTCGGACTCG GAGAGCCGCA TGAGCAAATC AAAGTCTGAC 
GGACAGTTAA GCGATAAAGT GGCCCTGGAG GCCAAAGTGA AAAATCTGTT AGGTCTGGCC 
AAAAGCAAAG ACGTGGAAAT CTTGCAGCTC AGGGGTGAGC TGAGGGACAT GAGGGTTCAG 
CTGGGACTGC CTGAGGAAGA TGAGGAGGAA GAGAGGCCAC CTGAGAGAGA GGCGGCTGCA 
GTGATCACAG CTGCGGATGT GGAGTCAACA TTACTGCTCT TGCAGGAGCA GAATCAGGCC 
ATCCGTGGAG AGCTTAACCT GCTCAAAAAT GAAAACCGCA TGCTGAAGGA CCGCCTCAAT 
GCTCTGGGCT TTTCCCTAGA GCAGAGACTG GACGGGGCTG ATAAGACCTT TGGCTTTCCC 
TCCACCAGTC CTGAACTCTC CTCTGGAGGC GCTGGAGCTC ATGGAGACTG CGCCACGGTG 
GCCTCCTCCG TTGAGGGATC GGCACCAGGC TCTATGGAGG ACCTCCTGAC AGGAGGCCAG 
CGAAGTGGCT CAACGGACAA CTTAGACAGC GAGTCCAGTG AGGTCTACCA AGCAGTGACC 
TCCAGTGATG ATGCCTTGGA TGCTCCTTCA GGCTGCGGTT CTTCATCATC CTCTGAGTCT 
GAAGGTGGCC CTCCGGCCTG CCGCAGTTCC TCTCGTAAAG GAAGCAGTGG CAACACCAGT 
GAGGTGTCCG TGGCGTGCCT GACAGAGCGC ATTCATCAGA TGGAGGAGAA CCAACACAGC 
ACATCAGAAG AGCTCCAAGC CACTCTACAG GAACTGGCTG ACTTACAGCA GATCACACAG 
GAGCTAAACG GCGAAAATGA ACGCCTGGGA GAAGAAAAGG TCCTGCTTAT GGACTCGCTG 
TGTCAACAGA GTGACAAACT AGAGCACTGT GGTCGTCAAA TAGAGTACTT TCGCTCACTC 
CTGGATGAGC ATGGTGTTGC CTACTCGGTA GATGAAGACA TCAAGAGTGG CCGATACATG 
GAGCTGGAGC AGCGCTACGT GGAGCTTGCG GAAAACGCCC GCTTTGAGCG CGAGCAGCTG 
CTAGGCGTGC AGCAGCATCT GAGTAACACT CTAAAGATGG CCGAGCAGGA CAACGCTGAG 
GCTCAAAATG TGATCGCAGC ACTGAAGGAG CGCAACCATC ACATGGAGCG ACTTCTGGAC 
GTGGAAAGGC AAGAGCGAGC CAGCATGGCG GCAGTGCTGG AGGAGTGTAA AGCTGCGGTC 
AACAACGACC AGGCTGAGCT GAGTCGCTGT CGAGTCTTAC TCGAGCAGGA GAGGCAAAAG 
GTTGCAGAAC TCTATTCTAT CCACAATGCT GGAGATAAGA GTGACATCCA CCAGCTCCTG 
GAGGGGGTGA GGCTGGACAA AGAGGAAGCT GAAGCCAAAG CCTCCAAACT ACAGGATGAC 
CTGGGCCATG CTCGTTCTGA GGTTGCATGT CTGCAGGACA CACTCAACAA GCTGGATGCA 
GAATACAGGG ACTTCCAGAG TGTAGTGCAA AAAGAGCTGG CTGAGCAAAA GAGGGCTATA 
GAGAAGCAAC GGGAGGACCT GCAGGAGAAA GAGACTGAGA TCGGAGATAT GAAAGAGACC 
ATCTTTGAGT TGGAGGATGA AGTGGAGCAG CACCGAGCCG TCAAACTTCA TGACAACCTC 
ATCATCAGCG ACCTGGAGAA TTCGATGAAA AAGCTTCAGG ATCAGAAGCA TGACATGGAG 
AGAGAGATAA AGATCCTGCA CCGCAGACTC CGGGAAGAGT CTGCAGAGTG GCGTCAGTTT 
CAGGCAGATC TGCAAACTGC TGTGGTCATT GCTAATGACA TCAAATCAGA GGCTCAAGAG 
GAGATTGGTG ACTTACGGCG CCGCCTGCAA GAAGCCCAAG AGAAGAATGA GAAGTTGGGC 
AAAGAAATAG AGGAAGTTAA AAACCGCAAG CAAGATGAAG AGCGTGGTCG TGTGTATAAC 
TACATGAACG CTGTGGAGAG GGATCTAGCT GCGCTCCGGC AGGGTATGGG ATTGAGTCGA 
CGACCGTCCA CATCCTCCGA ACCATCGCCC ACCGTTAAAA CTCTCATCAA GAGCTTCGAC 
AGTGCCTCAC AGGGTCCTGG AGCTAACGCT ACTGCTGTCG CTGCCGCCGC CGCTGCTGCA 
GCAGCCGCTG CTGCTGTCAC CGTCACAAGC ACAACACCTA CAGCCCCTTT ACCCAGAACA 
CCTCTGAGTC CCAGCCCAAT GAAAACCCCA CCTGCTGCTG CCGTCTCACC CATACAGAGA 
CACTCCATCA CTGGCTCCAT GGCTGCCGCA AAGCCTCTCT CCTCACTTGC AGACAAAAGA 
CCCAGTTACA CAGACATCAG CATGCCAGCT GAGCATCTAC TCAGAGCAGC CAACAGTCGT 
CCAGCTTCAG CTCTTCAGAG AGTCTCAAAT ATGGATACAT CAAAGACCAT CACAGTATCT 
CGGAGAAGCA GTGAGGAGCC GAAGCGGGAT ATCAGCACTC CTGATGGAGG TCCGGCTTCC 
TCTCTGATAA GCATGAGCTC TGCTGCCGCG CTGTCCTCTT CTTCCTCTCC CACAGCCTCC 
GTCAACCCCA CTGCACGAAG CCGCCTTCGA GAGGAGCGAA AAGACCCTTT GTCAGCTTTG 
GCTCGAGAGT ATGGTGGTTC CAAAAGAAAT GCACTGTTGA GGTGGTGTCA GAAGAAGACT 
GAGGGTTATC AGAACATTGA CATTACTAAC TTCAGCAGCA GCTGGAATGA TGGACTTGCC 
TTCTGTGCTG TCCTTCACAC GTACCTGCCT GCTCACATTC CCTACCAAGA GCTCAACAGT 
CAAGACAAGA GACGAAATTT CACCCTGGCC TTCCAAGCAG CAGAAAGTGT GGGCATCAAG 
TCCACTCTGG ACATCAATGA CATGGTCCAT ACAGAAAGAC CGGACTGGCA GAGTGTTATG 
ACTTACGTCA CCTCCATCTA CAAGTATTTT GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034905.1
CDS	38..3436
Translation	MKKAGRPVGNKAASGGGKGDSVTAGNCAGKNTVKSPTSASLSKVKSNDDLLAAMAGSSAGTNNSVAKGKKSTSTHSTSCGTNTNNPDTKTKTTSGPSGKRTTSMTSKESNSSRERLRNSRNSSSKKQSSTGASDRAQPKHSRALAQTSDSESRMSKSKSDGQLSDKVALEAKVKNLLGLAKSKDVEILQLRGELRDMRVQLGLPEEDEEEERPPEREAAAVITAADVESTLLLLQEQNQAIRGELNLLKNENRMLKDRLNALGFSLEQRLDGADKTFGFPSTSPELSSGGAGAHGDCATVASSVEGSAPGSMEDLLTGGQRSGSTDNLDSESSEVYQAVTSSDDALDAPSGCGSSSSSESEGGPPACRSSSRKGSSGNTSEVSVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNGENERLGEEKVLLMDSLCQQSDKLEHCGRQIEYFRSLLDEHGVAYSVDEDIKSGRYMELEQRYVELAENARFEREQLLGVQQHLSNTLKMAEQDNAEAQNVIAALKERNHHMERLLDVERQERASMAAVLEECKAAVNNDQAELSRCRVLLEQERQKVAELYSIHNAGDKSDIHQLLEGVRLDKEEAEAKASKLQDDLGHARSEVACLQDTLNKLDAEYRDFQSVVQKELAEQKRAIEKQREDLQEKETEIGDMKETIFELEDEVEQHRAVKLHDNLIISDLENSMKKLQDQKHDMEREIKILHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLRRRLQEAQEKNEKLGKEIEEVKNRKQDEERGRVYNYMNAVERDLAALRQGMGLSRRPSTSSEPSPTVKTLIKSFDSASQGPGANATAVAAAAAAAAAAAAVTVTSTTPTAPLPRTPLSPSPMKTPPAAAVSPIQRHSITGSMAAAKPLSSLADKRPSYTDISMPAEHLLRAANSRPASALQRVSNMDTSKTITVSRRSSEEPKRDISTPDGGPASSLISMSSAAALSSSSSPTASVNPTARSRLREERKDPLSALAREYGGSKRNALLRWCQKKTEGYQNIDITNFSSSWNDGLAFCAVLHTYLPAHIPYQELNSQDKRRNFTLAFQAAESVGIKSTLDINDMVHTERPDWQSVMTYVTSIYKYFET

Target ORF information:

RefSeq Version	NM_001039816.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sperm antigen with calponin homology and coiled-coil domains 1-like a (specc1la), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039816.1

ORF Insert Sequence:

ATGAAGAAAG CAGGCAGGCC AGTGGGGAAT AAGGCAGCAA GTGGAGGAGG TAAAGGTGAC 
TCAGTGACGG CCGGAAACTG TGCTGGCAAA AACACTGTTA AGAGCCCTAC ATCAGCTTCT 
CTCTCCAAGG TGAAAAGCAA TGATGATCTT CTAGCTGCTA TGGCTGGTAG CAGTGCAGGC 
ACAAACAACA GTGTTGCCAA GGGCAAGAAG TCAACGTCTA CTCACTCAAC ATCTTGTGGA 
ACAAACACTA ATAACCCTGA CACCAAGACC AAAACCACCT CAGGTCCTTC TGGCAAACGG 
ACAACATCCA TGACCTCCAA GGAGTCGAAT TCCTCTCGAG AGCGTTTAAG AAACTCTAGA 
AACTCCAGCA GTAAAAAGCA GTCGTCCACT GGTGCTTCAG ATAGAGCTCA GCCCAAGCAT 
TCCCGTGCAC TGGCCCAGAC CTCGGACTCG GAGAGCCGCA TGAGCAAATC AAAGTCTGAC 
GGACAGTTAA GCGATAAAGT GGCCCTGGAG GCCAAAGTGA AAAATCTGTT AGGTCTGGCC 
AAAAGCAAAG ACGTGGAAAT CTTGCAGCTC AGGGGTGAGC TGAGGGACAT GAGGGTTCAG 
CTGGGACTGC CTGAGGAAGA TGAGGAGGAA GAGAGGCCAC CTGAGAGAGA GGCGGCTGCA 
GTGATCACAG CTGCGGATGT GGAGTCAACA TTACTGCTCT TGCAGGAGCA GAATCAGGCC 
ATCCGTGGAG AGCTTAACCT GCTCAAAAAT GAAAACCGCA TGCTGAAGGA CCGCCTCAAT 
GCTCTGGGCT TTTCCCTAGA GCAGAGACTG GACGGGGCTG ATAAGACCTT TGGCTTTCCC 
TCCACCAGTC CTGAACTCTC CTCTGGAGGC GCTGGAGCTC ATGGAGACTG CGCCACGGTG 
GCCTCCTCCG TTGAGGGATC GGCACCAGGC TCTATGGAGG ACCTCCTGAC AGGAGGCCAG 
CGAAGTGGCT CAACGGACAA CTTAGACAGC GAGTCCAGTG AGGTCTACCA AGCAGTGACC 
TCCAGTGATG ATGCCTTGGA TGCTCCTTCA GGCTGCGGTT CTTCATCATC CTCTGAGTCT 
GAAGGTGGCC CTCCGGCCTG CCGCAGTTCC TCTCGTAAAG GAAGCAGTGG CAACACCAGT 
GAGGTGTCCG TGGCGTGCCT GACAGAGCGC ATTCATCAGA TGGAGGAGAA CCAACACAGC 
ACATCAGAAG AGCTCCAAGC CACTCTACAG GAACTGGCTG ACTTACAGCA GATCACACAG 
GAGCTAAACG GCGAAAATGA ACGCCTGGGA GAAGAAAAGG TCCTGCTTAT GGACTCGCTG 
TGTCAACAGA GTGACAAACT AGAGCACTGT GGTCGTCAAA TAGAGTACTT TCGCTCACTC 
CTGGATGAGC ATGGTGTTGC CTACTCGGTA GATGAAGACA TCAAGAGTGG CCGATACATG 
GAGCTGGAGC AGCGCTACGT GGAGCTTGCG GAAAACGCCC GCTTTGAGCG CGAGCAGCTG 
CTAGGCGTGC AGCAGCATCT GAGTAACACT CTAAAGATGG CCGAGCAGGA CAACGCTGAG 
GCTCAAAATG TGATCGCAGC ACTGAAGGAG CGCAACCATC ACATGGAGCG ACTTCTGGAC 
GTGGAAAGGC AAGAGCGAGC CAGCATGGCG GCAGTGCTGG AGGAGTGTAA AGCTGCGGTC 
AACAACGACC AGGCTGAGCT GAGTCGCTGT CGAGTCTTAC TCGAGCAGGA GAGGCAAAAG 
GTTGCAGAAC TCTATTCTAT CCACAATGCT GGAGATAAGA GTGACATCCA CCAGCTCCTG 
GAGGGGGTGA GGCTGGACAA AGAGGAAGCT GAAGCCAAAG CCTCCAAACT ACAGGATGAC 
CTGGGCCATG CTCGTTCTGA GGTTGCATGT CTGCAGGACA CACTCAACAA GCTGGATGCA 
GAATACAGGG ACTTCCAGAG TGTAGTGCAA AAAGAGCTGG CTGAGCAAAA GAGGGCTATA 
GAGAAGCAAC GGGAGGACCT GCAGGAGAAA GAGACTGAGA TCGGAGATAT GAAAGAGACC 
ATCTTTGAGT TGGAGGATGA AGTGGAGCAG CACCGAGCCG TCAAACTTCA TGACAACCTC 
ATCATCAGCG ACCTGGAGAA TTCGATGAAA AAGCTTCAGG ATCAGAAGCA TGACATGGAG 
AGAGAGATAA AGATCCTGCA CCGCAGACTC CGGGAAGAGT CTGCAGAGTG GCGTCAGTTT 
CAGGCAGATC TGCAAACTGC TGTGGTCATT GCTAATGACA TCAAATCAGA GGCTCAAGAG 
GAGATTGGTG ACTTACGGCG CCGCCTGCAA GAAGCCCAAG AGAAGAATGA GAAGTTGGGC 
AAAGAAATAG AGGAAGTTAA AAACCGCAAG CAAGATGAAG AGCGTGGTCG TGTGTATAAC 
TACATGAACG CTGTGGAGAG GGATCTAGCT GCGCTCCGGC AGGGTATGGG ATTGAGTCGA 
CGACCGTCCA CATCCTCCGA ACCATCGCCC ACCGTTAAAA CTCTCATCAA GAGCTTCGAC 
AGTGCCTCAC AGGGTCCTGG AGCTAACGCT ACTGCTGTCG CTGCCGCCGC CGCTGCTGCA 
GCAGCCGCTG CTGCTGTCAC CGTCACAAGC ACAACACCTA CAGCCCCTTT ACCCAGAACA 
CCTCTGAGTC CCAGCCCAAT GAAAACCCCA CCTGCTGCTG CCGTCTCACC CATACAGAGA 
CACTCCATCA CTGGCTCCAT GGCTGCCGCA AAGCCTCTCT CCTCACTTGC AGACAAAAGA 
CCCAGTTACA CAGACATCAG CATGCCAGCT GAGCATCTAC TCAGAGCAGC CAACAGTCGT 
CCAGCTTCAG CTCTTCAGAG AGTCTCAAAT ATGGATACAT CAAAGACCAT CACAGTATCT 
CGGAGAAGCA GTGAGGAGCC GAAGCGGGAT ATCAGCACTC CTGATGGAGG TCCGGCTTCC 
TCTCTGATAA GCATGAGCTC TGCTGCCGCG CTGTCCTCTT CTTCCTCTCC CACAGCCTCC 
GTCAACCCCA CTGCACGAAG CCGCCTTCGA GAGGAGCGAA AAGACCCTTT GTCAGCTTTG 
GCTCGAGAGT ATGGTGGTTC CAAAAGAAAT GCACTGTTGA GGTGGTGTCA GAAGAAGACT 
GAGGGTTATC AGAACATTGA CATTACTAAC TTCAGCAGCA GCTGGAATGA TGGACTTGCC 
TTCTGTGCTG TCCTTCACAC GTACCTGCCT GCTCACATTC CCTACCAAGA GCTCAACAGT 
CAAGACAAGA GACGAAATTT CACCCTGGCC TTCCAAGCAG CAGAAAGTGT GGGCATCAAG 
TCCACTCTGG ACATCAATGA CATGGTCCAT ACAGAAAGAC CGGACTGGCA GAGTGTTATG 
ACTTACGTCA CCTCCATCTA CAAGTATTTT GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

specc1la ( XM_005167177.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167234.1 Danio rerio sperm antigen with calponin homology and coiled-coil domains 1-like a (specc1la), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa14378
Clone ID Related Accession (Same CDS sequence)	XM_005167177.4 , XM_005167177.3
Accession Version	XM_005167177.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cytospin-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167177.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG CAGGCAGGCC AGTGGGGAAT AAGGCAGCAA GTGGAGGAGG TAAAGGTGAC 
TCAGTGACGG CCGGAAACTG TGCTGGCAAA AACACTGTTA AGAGCCCTAC ATCAGCTCCT 
CTCTCCAAGG TGAAAAGCAA TGATGATCTT CTAGCTGCTA TGGCTGGTAG CAGTGCAGGC 
ACAAACAACA GTGTTGCCAA GGGCAAGAAG TCAACGTCTA CTCACTCAAC ATCTTGTGGA 
ACAAACACTA ATAACCCTGA CACCAAGACC AAAACCACCT CAGGTCCTTC TGGCAAACGG 
ACAACATCCA TGACCTCCAA GGAGTCGAAT TCCTCACGAG AGCGTTTAAG AAACTCTAGA 
AACTCCAGCA GTAAAAAGCA GTCGTCCACT GGTGCTTCAG ATAGAGCTCA GCCCAAGCAT 
TCCCGTGCAC TGGCCCAGAC CTCGGACTCG GAGAGCCGCA TGAGCAAATC AAAGTCTGAC 
GGACAGTTAA GCGATAAAGT GGCCCTGGAG GCCAAAGTGA AAAATCTGTT AGGTCTGGCC 
AAAAGCAAAG ACGTGGAAAT CTTGCAGCTC AGGGGTGAGC TGAGGGACAT GAGGGTTCAG 
CTGGGACTGC CTGAGGAAGA TGAGGAGGAA GAGAGGCCAC CTGAGAGAGA GGCGGCTGCA 
GTGATCACAG CTGCGGATGT GGAGTCAACA TTACTGCTCT TGCAGGAGCA GAATCAGGCC 
ATCCGTGGAG AGCTTAACCT GCTCAAAAAT GAAAACCGCA TGCTGAAGGA CCGCCTCAAT 
GCTCTGGGCT TTTCCCTAGA GCAGAGACTG GACGGGGCTG ATAAGACCTT TGGCTTTCCC 
TCCACCAGTC CTGAACTCTC CTCTGGAGGT GCTGGAGCTC ATGGAGACTG CGCCACGGTG 
GCCTCCTCCG TTGAGGGATC GGCACCAGGC TCTATGGAGG ACCTCCTGAC AGGAGGCCAG 
CGAAGTGGCT CAACGGACAA CTTAGACAGC GAGTCCAGTG AGGTCTACCA AGCAGTGACC 
TCCAGTGATG ATGCCTTGGA TGCTCCTTCA GGCTGCGGTT CTTCATCATC CTCTGAGTCT 
GAAGGTGGCC CTCCGGCCTG CCGCAGTTCC TCTCGTAAAG GAAGCAGTGG CAACACCAGT 
GAGGTGTCCG TGGCGTGCCT GACAGAGCGC ATTCATCAGA TGGAGGAGAA CCAACACAGC 
ACATCAGAAG AGCTCCAAGC CACTCTACAG GAACTGGCTG ACTTACAGCA GATCACACAG 
GAGCTAAACG GCGAAAATGA ACGCCTGGGA GAAGAAAAGG TCCTGCTTAT GGACTCTCTG 
TGTCAACAGA GTGACAAGCT AGAGCACTGT GGTCGTCAAA TAGAGTACTT TCGCTCACTC 
CTGGATGAGC ATGGTGTTGC CTACTCGGTA GATGAAGACA TCAAGAGTGG CCGATACATG 
GAGCTGGAGC AGCGCTACGT TGAGCTTGCG GAAAACGCCC GCTTTGAGCG TGAGCAGCTG 
TTAGGCGTGC AGCAGCATCT GAGTAACACT CTAAAGATGG CCGAACAGGA CAACGCTGAG 
GCTCAAAATG TGATCGCAGC ACTGAAGGAG CGCAACCATC ACATGGAGCG ACTTCTGGAC 
GTGGAAAGGC AAGAGCGAGC CAGCATGGCG GCAGTGCTGG AGGAGTGTAA AGCTGCGGTC 
AACAACGACC AGGCTGAGCT GAGTCGCTGT CGGGTCTTAC TCGAGCAGGA GAGACAAAAG 
GTTGCAGAAC TCTATTCCAT CCACAATGCT GGAGATAAGA GTGACATCCA CCAGCTCCTG 
GAGGGGGTGA GGCTGGACAA AGAGGAAGCT GAAGCCAAAG CCTCCAAACT ACAGGATGAC 
CTGGGCCATG CTCGCTCTGA GGTTGCATGT CTGCAGGACA CACTCAACAA GCTGGATGCA 
GAGTACAGGG ACTTCCAGAG TGTAGTGCAA AAAGAGCTGG CTGAGCAAAA GAGGGCTATA 
GAGAAGCAAC GGGAGGACCT GCAGGAGAAA GAGACTGAGA TCGGAGATAT GAAAGAGACC 
ATCTTTGAGT TGGAGGATGA AGTGGAGCAG CACCGAGCCG TCAAACTTCA TGACAACCTC 
ATCATCAGCG ACCTGGAGAA TTCGATGAAA AAGCTTCAGG ATCAGAAGCA TGACATGGAG 
AGAGAGATAA AGATCCTGCA CCGCAGACTC CGGGAAGAGT CTGCAGAGTG GCGTCAGTTT 
CAGGCAGATC TGCAAACTGC TGTGGTCATT GCTAATGACA TCAAATCAGA GGCTCAAGAG 
GAGATTGGTG ACTTACGGCG CCGCCTGCAA GAAGCCCAAG AGAAGAATGA GAAGTTGGGC 
AAAGAAATAG AGGAAGTTAA AAACCGCAAG CAAGATGAAG AGCGTGGTCG TGTGTATAAC 
TACATGAACG CTGTGGAGAG GGATCTAGCT GCGCTCCGGC AGGGTATGGG ATTGAGTCGA 
CGACCGTCCA CATCCTCCGA ACCATCGCCC ACCGTTAAAA CTCTCATCAA GAGCTTCGAC 
AGTGCCTCAC AGGGTCCTGG AGCTAACGCT ACTGCTGTCG CTGCCGCCGC CGCTGCTGCA 
GCAGCCGCTG CTGCTGTCAC CGTCACAAGC ACAACACCTA CAGCCCCTTT ACCCAGAACA 
CCTCTGAGTC CCAGCCCAAT GAAAACCCCA CCTGCTGCTG CCGTCTCACC CATACAGAGA 
CACTCCATCA CTGGCTCCAT GGCTGCCGCA AAGCCTCTCT CCTCACTTGC AGACAAAAGA 
CCCAGTTACA CAGACATCAG CATGCCAGCT GAGCATCTAC TCAGAGCAGC CAACAGTCGT 
CCAGCTTCAG CTCTTCAGAG AGTCTCAAAT ATGGATACAT CAAAGACCAT CACAGTATCT 
CGGAGAAGCA GTGAGGAGCC GAAGCGGGAT ATCAGCACTC CTGATGGAGG TCCGGCTTCC 
TCTCTGATAA GCATGAGCTC TGCTGCCGCG CTGTCCTCTT CTTCCTCTCC CACAGCCTCC 
GTCAACCCCA CTGCACGAAG CCGCCTTCGA GAGGAGCGAA AAGACCCTTT GTCAGCTTTG 
GCTCGAGAGT ATGGTGGTTC CAAAAGAAAT GCACTGTTGA GGTGGTGTCA GAAGAAGACT 
GAGGGTTATC AGAACATTGA CATTACTAAC TTCAGCAGCA GCTGGAATGA TGGACTTGCC 
TTCTGTGCTG TCCTTCACAC GTACCTGCCT GCTCACATTC CCTACCAAGA GCTCAACAGT 
CAAGACAAGA GACGAAATTT CACCCTGGCC TTCCAAGCAG CAGAAAGTGT GGGCATCAAG 
TCCACTCTGG ACATCACTGA CATGGTCCAT ACAGAAAGAC CGGACTGGCA GAGTGTTATG 
ACTTACGTCA CCTCCATCTA CAAGTATTTT GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167234.1
CDS	230..3628
Translation	MKKAGRPVGNKAASGGGKGDSVTAGNCAGKNTVKSPTSAPLSKVKSNDDLLAAMAGSSAGTNNSVAKGKKSTSTHSTSCGTNTNNPDTKTKTTSGPSGKRTTSMTSKESNSSRERLRNSRNSSSKKQSSTGASDRAQPKHSRALAQTSDSESRMSKSKSDGQLSDKVALEAKVKNLLGLAKSKDVEILQLRGELRDMRVQLGLPEEDEEEERPPEREAAAVITAADVESTLLLLQEQNQAIRGELNLLKNENRMLKDRLNALGFSLEQRLDGADKTFGFPSTSPELSSGGAGAHGDCATVASSVEGSAPGSMEDLLTGGQRSGSTDNLDSESSEVYQAVTSSDDALDAPSGCGSSSSSESEGGPPACRSSSRKGSSGNTSEVSVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNGENERLGEEKVLLMDSLCQQSDKLEHCGRQIEYFRSLLDEHGVAYSVDEDIKSGRYMELEQRYVELAENARFEREQLLGVQQHLSNTLKMAEQDNAEAQNVIAALKERNHHMERLLDVERQERASMAAVLEECKAAVNNDQAELSRCRVLLEQERQKVAELYSIHNAGDKSDIHQLLEGVRLDKEEAEAKASKLQDDLGHARSEVACLQDTLNKLDAEYRDFQSVVQKELAEQKRAIEKQREDLQEKETEIGDMKETIFELEDEVEQHRAVKLHDNLIISDLENSMKKLQDQKHDMEREIKILHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLRRRLQEAQEKNEKLGKEIEEVKNRKQDEERGRVYNYMNAVERDLAALRQGMGLSRRPSTSSEPSPTVKTLIKSFDSASQGPGANATAVAAAAAAAAAAAAVTVTSTTPTAPLPRTPLSPSPMKTPPAAAVSPIQRHSITGSMAAAKPLSSLADKRPSYTDISMPAEHLLRAANSRPASALQRVSNMDTSKTITVSRRSSEEPKRDISTPDGGPASSLISMSSAAALSSSSSPTASVNPTARSRLREERKDPLSALAREYGGSKRNALLRWCQKKTEGYQNIDITNFSSSWNDGLAFCAVLHTYLPAHIPYQELNSQDKRRNFTLAFQAAESVGIKSTLDITDMVHTERPDWQSVMTYVTSIYKYFET

Target ORF information:

RefSeq Version	XM_005167177.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sperm antigen with calponin homology and coiled-coil domains 1-like a (specc1la), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167177.4

ORF Insert Sequence:

ATGAAGAAAG CAGGCAGGCC AGTGGGGAAT AAGGCAGCAA GTGGAGGAGG TAAAGGTGAC 
TCAGTGACGG CCGGAAACTG TGCTGGCAAA AACACTGTTA AGAGCCCTAC ATCAGCTCCT 
CTCTCCAAGG TGAAAAGCAA TGATGATCTT CTAGCTGCTA TGGCTGGTAG CAGTGCAGGC 
ACAAACAACA GTGTTGCCAA GGGCAAGAAG TCAACGTCTA CTCACTCAAC ATCTTGTGGA 
ACAAACACTA ATAACCCTGA CACCAAGACC AAAACCACCT CAGGTCCTTC TGGCAAACGG 
ACAACATCCA TGACCTCCAA GGAGTCGAAT TCCTCACGAG AGCGTTTAAG AAACTCTAGA 
AACTCCAGCA GTAAAAAGCA GTCGTCCACT GGTGCTTCAG ATAGAGCTCA GCCCAAGCAT 
TCCCGTGCAC TGGCCCAGAC CTCGGACTCG GAGAGCCGCA TGAGCAAATC AAAGTCTGAC 
GGACAGTTAA GCGATAAAGT GGCCCTGGAG GCCAAAGTGA AAAATCTGTT AGGTCTGGCC 
AAAAGCAAAG ACGTGGAAAT CTTGCAGCTC AGGGGTGAGC TGAGGGACAT GAGGGTTCAG 
CTGGGACTGC CTGAGGAAGA TGAGGAGGAA GAGAGGCCAC CTGAGAGAGA GGCGGCTGCA 
GTGATCACAG CTGCGGATGT GGAGTCAACA TTACTGCTCT TGCAGGAGCA GAATCAGGCC 
ATCCGTGGAG AGCTTAACCT GCTCAAAAAT GAAAACCGCA TGCTGAAGGA CCGCCTCAAT 
GCTCTGGGCT TTTCCCTAGA GCAGAGACTG GACGGGGCTG ATAAGACCTT TGGCTTTCCC 
TCCACCAGTC CTGAACTCTC CTCTGGAGGT GCTGGAGCTC ATGGAGACTG CGCCACGGTG 
GCCTCCTCCG TTGAGGGATC GGCACCAGGC TCTATGGAGG ACCTCCTGAC AGGAGGCCAG 
CGAAGTGGCT CAACGGACAA CTTAGACAGC GAGTCCAGTG AGGTCTACCA AGCAGTGACC 
TCCAGTGATG ATGCCTTGGA TGCTCCTTCA GGCTGCGGTT CTTCATCATC CTCTGAGTCT 
GAAGGTGGCC CTCCGGCCTG CCGCAGTTCC TCTCGTAAAG GAAGCAGTGG CAACACCAGT 
GAGGTGTCCG TGGCGTGCCT GACAGAGCGC ATTCATCAGA TGGAGGAGAA CCAACACAGC 
ACATCAGAAG AGCTCCAAGC CACTCTACAG GAACTGGCTG ACTTACAGCA GATCACACAG 
GAGCTAAACG GCGAAAATGA ACGCCTGGGA GAAGAAAAGG TCCTGCTTAT GGACTCTCTG 
TGTCAACAGA GTGACAAGCT AGAGCACTGT GGTCGTCAAA TAGAGTACTT TCGCTCACTC 
CTGGATGAGC ATGGTGTTGC CTACTCGGTA GATGAAGACA TCAAGAGTGG CCGATACATG 
GAGCTGGAGC AGCGCTACGT TGAGCTTGCG GAAAACGCCC GCTTTGAGCG TGAGCAGCTG 
TTAGGCGTGC AGCAGCATCT GAGTAACACT CTAAAGATGG CCGAACAGGA CAACGCTGAG 
GCTCAAAATG TGATCGCAGC ACTGAAGGAG CGCAACCATC ACATGGAGCG ACTTCTGGAC 
GTGGAAAGGC AAGAGCGAGC CAGCATGGCG GCAGTGCTGG AGGAGTGTAA AGCTGCGGTC 
AACAACGACC AGGCTGAGCT GAGTCGCTGT CGGGTCTTAC TCGAGCAGGA GAGACAAAAG 
GTTGCAGAAC TCTATTCCAT CCACAATGCT GGAGATAAGA GTGACATCCA CCAGCTCCTG 
GAGGGGGTGA GGCTGGACAA AGAGGAAGCT GAAGCCAAAG CCTCCAAACT ACAGGATGAC 
CTGGGCCATG CTCGCTCTGA GGTTGCATGT CTGCAGGACA CACTCAACAA GCTGGATGCA 
GAGTACAGGG ACTTCCAGAG TGTAGTGCAA AAAGAGCTGG CTGAGCAAAA GAGGGCTATA 
GAGAAGCAAC GGGAGGACCT GCAGGAGAAA GAGACTGAGA TCGGAGATAT GAAAGAGACC 
ATCTTTGAGT TGGAGGATGA AGTGGAGCAG CACCGAGCCG TCAAACTTCA TGACAACCTC 
ATCATCAGCG ACCTGGAGAA TTCGATGAAA AAGCTTCAGG ATCAGAAGCA TGACATGGAG 
AGAGAGATAA AGATCCTGCA CCGCAGACTC CGGGAAGAGT CTGCAGAGTG GCGTCAGTTT 
CAGGCAGATC TGCAAACTGC TGTGGTCATT GCTAATGACA TCAAATCAGA GGCTCAAGAG 
GAGATTGGTG ACTTACGGCG CCGCCTGCAA GAAGCCCAAG AGAAGAATGA GAAGTTGGGC 
AAAGAAATAG AGGAAGTTAA AAACCGCAAG CAAGATGAAG AGCGTGGTCG TGTGTATAAC 
TACATGAACG CTGTGGAGAG GGATCTAGCT GCGCTCCGGC AGGGTATGGG ATTGAGTCGA 
CGACCGTCCA CATCCTCCGA ACCATCGCCC ACCGTTAAAA CTCTCATCAA GAGCTTCGAC 
AGTGCCTCAC AGGGTCCTGG AGCTAACGCT ACTGCTGTCG CTGCCGCCGC CGCTGCTGCA 
GCAGCCGCTG CTGCTGTCAC CGTCACAAGC ACAACACCTA CAGCCCCTTT ACCCAGAACA 
CCTCTGAGTC CCAGCCCAAT GAAAACCCCA CCTGCTGCTG CCGTCTCACC CATACAGAGA 
CACTCCATCA CTGGCTCCAT GGCTGCCGCA AAGCCTCTCT CCTCACTTGC AGACAAAAGA 
CCCAGTTACA CAGACATCAG CATGCCAGCT GAGCATCTAC TCAGAGCAGC CAACAGTCGT 
CCAGCTTCAG CTCTTCAGAG AGTCTCAAAT ATGGATACAT CAAAGACCAT CACAGTATCT 
CGGAGAAGCA GTGAGGAGCC GAAGCGGGAT ATCAGCACTC CTGATGGAGG TCCGGCTTCC 
TCTCTGATAA GCATGAGCTC TGCTGCCGCG CTGTCCTCTT CTTCCTCTCC CACAGCCTCC 
GTCAACCCCA CTGCACGAAG CCGCCTTCGA GAGGAGCGAA AAGACCCTTT GTCAGCTTTG 
GCTCGAGAGT ATGGTGGTTC CAAAAGAAAT GCACTGTTGA GGTGGTGTCA GAAGAAGACT 
GAGGGTTATC AGAACATTGA CATTACTAAC TTCAGCAGCA GCTGGAATGA TGGACTTGCC 
TTCTGTGCTG TCCTTCACAC GTACCTGCCT GCTCACATTC CCTACCAAGA GCTCAACAGT 
CAAGACAAGA GACGAAATTT CACCCTGGCC TTCCAAGCAG CAGAAAGTGT GGGCATCAAG 
TCCACTCTGG ACATCACTGA CATGGTCCAT ACAGAAAGAC CGGACTGGCA GAGTGTTATG 
ACTTACGTCA CCTCCATCTA CAAGTATTTT GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

specc1la ( XM_005167177.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167234.1 Danio rerio sperm antigen with calponin homology and coiled-coil domains 1-like a (specc1la), transcript variant X1, mRNA.

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CloneID	ODa14378
Clone ID Related Accession (Same CDS sequence)	XM_005167177.4 , XM_005167177.3
Accession Version	XM_005167177.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	cytospin-A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167177.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG CAGGCAGGCC AGTGGGGAAT AAGGCAGCAA GTGGAGGAGG TAAAGGTGAC 
TCAGTGACGG CCGGAAACTG TGCTGGCAAA AACACTGTTA AGAGCCCTAC ATCAGCTCCT 
CTCTCCAAGG TGAAAAGCAA TGATGATCTT CTAGCTGCTA TGGCTGGTAG CAGTGCAGGC 
ACAAACAACA GTGTTGCCAA GGGCAAGAAG TCAACGTCTA CTCACTCAAC ATCTTGTGGA 
ACAAACACTA ATAACCCTGA CACCAAGACC AAAACCACCT CAGGTCCTTC TGGCAAACGG 
ACAACATCCA TGACCTCCAA GGAGTCGAAT TCCTCACGAG AGCGTTTAAG AAACTCTAGA 
AACTCCAGCA GTAAAAAGCA GTCGTCCACT GGTGCTTCAG ATAGAGCTCA GCCCAAGCAT 
TCCCGTGCAC TGGCCCAGAC CTCGGACTCG GAGAGCCGCA TGAGCAAATC AAAGTCTGAC 
GGACAGTTAA GCGATAAAGT GGCCCTGGAG GCCAAAGTGA AAAATCTGTT AGGTCTGGCC 
AAAAGCAAAG ACGTGGAAAT CTTGCAGCTC AGGGGTGAGC TGAGGGACAT GAGGGTTCAG 
CTGGGACTGC CTGAGGAAGA TGAGGAGGAA GAGAGGCCAC CTGAGAGAGA GGCGGCTGCA 
GTGATCACAG CTGCGGATGT GGAGTCAACA TTACTGCTCT TGCAGGAGCA GAATCAGGCC 
ATCCGTGGAG AGCTTAACCT GCTCAAAAAT GAAAACCGCA TGCTGAAGGA CCGCCTCAAT 
GCTCTGGGCT TTTCCCTAGA GCAGAGACTG GACGGGGCTG ATAAGACCTT TGGCTTTCCC 
TCCACCAGTC CTGAACTCTC CTCTGGAGGT GCTGGAGCTC ATGGAGACTG CGCCACGGTG 
GCCTCCTCCG TTGAGGGATC GGCACCAGGC TCTATGGAGG ACCTCCTGAC AGGAGGCCAG 
CGAAGTGGCT CAACGGACAA CTTAGACAGC GAGTCCAGTG AGGTCTACCA AGCAGTGACC 
TCCAGTGATG ATGCCTTGGA TGCTCCTTCA GGCTGCGGTT CTTCATCATC CTCTGAGTCT 
GAAGGTGGCC CTCCGGCCTG CCGCAGTTCC TCTCGTAAAG GAAGCAGTGG CAACACCAGT 
GAGGTGTCCG TGGCGTGCCT GACAGAGCGC ATTCATCAGA TGGAGGAGAA CCAACACAGC 
ACATCAGAAG AGCTCCAAGC CACTCTACAG GAACTGGCTG ACTTACAGCA GATCACACAG 
GAGCTAAACG GCGAAAATGA ACGCCTGGGA GAAGAAAAGG TCCTGCTTAT GGACTCTCTG 
TGTCAACAGA GTGACAAGCT AGAGCACTGT GGTCGTCAAA TAGAGTACTT TCGCTCACTC 
CTGGATGAGC ATGGTGTTGC CTACTCGGTA GATGAAGACA TCAAGAGTGG CCGATACATG 
GAGCTGGAGC AGCGCTACGT TGAGCTTGCG GAAAACGCCC GCTTTGAGCG TGAGCAGCTG 
TTAGGCGTGC AGCAGCATCT GAGTAACACT CTAAAGATGG CCGAACAGGA CAACGCTGAG 
GCTCAAAATG TGATCGCAGC ACTGAAGGAG CGCAACCATC ACATGGAGCG ACTTCTGGAC 
GTGGAAAGGC AAGAGCGAGC CAGCATGGCG GCAGTGCTGG AGGAGTGTAA AGCTGCGGTC 
AACAACGACC AGGCTGAGCT GAGTCGCTGT CGGGTCTTAC TCGAGCAGGA GAGACAAAAG 
GTTGCAGAAC TCTATTCCAT CCACAATGCT GGAGATAAGA GTGACATCCA CCAGCTCCTG 
GAGGGGGTGA GGCTGGACAA AGAGGAAGCT GAAGCCAAAG CCTCCAAACT ACAGGATGAC 
CTGGGCCATG CTCGCTCTGA GGTTGCATGT CTGCAGGACA CACTCAACAA GCTGGATGCA 
GAGTACAGGG ACTTCCAGAG TGTAGTGCAA AAAGAGCTGG CTGAGCAAAA GAGGGCTATA 
GAGAAGCAAC GGGAGGACCT GCAGGAGAAA GAGACTGAGA TCGGAGATAT GAAAGAGACC 
ATCTTTGAGT TGGAGGATGA AGTGGAGCAG CACCGAGCCG TCAAACTTCA TGACAACCTC 
ATCATCAGCG ACCTGGAGAA TTCGATGAAA AAGCTTCAGG ATCAGAAGCA TGACATGGAG 
AGAGAGATAA AGATCCTGCA CCGCAGACTC CGGGAAGAGT CTGCAGAGTG GCGTCAGTTT 
CAGGCAGATC TGCAAACTGC TGTGGTCATT GCTAATGACA TCAAATCAGA GGCTCAAGAG 
GAGATTGGTG ACTTACGGCG CCGCCTGCAA GAAGCCCAAG AGAAGAATGA GAAGTTGGGC 
AAAGAAATAG AGGAAGTTAA AAACCGCAAG CAAGATGAAG AGCGTGGTCG TGTGTATAAC 
TACATGAACG CTGTGGAGAG GGATCTAGCT GCGCTCCGGC AGGGTATGGG ATTGAGTCGA 
CGACCGTCCA CATCCTCCGA ACCATCGCCC ACCGTTAAAA CTCTCATCAA GAGCTTCGAC 
AGTGCCTCAC AGGGTCCTGG AGCTAACGCT ACTGCTGTCG CTGCCGCCGC CGCTGCTGCA 
GCAGCCGCTG CTGCTGTCAC CGTCACAAGC ACAACACCTA CAGCCCCTTT ACCCAGAACA 
CCTCTGAGTC CCAGCCCAAT GAAAACCCCA CCTGCTGCTG CCGTCTCACC CATACAGAGA 
CACTCCATCA CTGGCTCCAT GGCTGCCGCA AAGCCTCTCT CCTCACTTGC AGACAAAAGA 
CCCAGTTACA CAGACATCAG CATGCCAGCT GAGCATCTAC TCAGAGCAGC CAACAGTCGT 
CCAGCTTCAG CTCTTCAGAG AGTCTCAAAT ATGGATACAT CAAAGACCAT CACAGTATCT 
CGGAGAAGCA GTGAGGAGCC GAAGCGGGAT ATCAGCACTC CTGATGGAGG TCCGGCTTCC 
TCTCTGATAA GCATGAGCTC TGCTGCCGCG CTGTCCTCTT CTTCCTCTCC CACAGCCTCC 
GTCAACCCCA CTGCACGAAG CCGCCTTCGA GAGGAGCGAA AAGACCCTTT GTCAGCTTTG 
GCTCGAGAGT ATGGTGGTTC CAAAAGAAAT GCACTGTTGA GGTGGTGTCA GAAGAAGACT 
GAGGGTTATC AGAACATTGA CATTACTAAC TTCAGCAGCA GCTGGAATGA TGGACTTGCC 
TTCTGTGCTG TCCTTCACAC GTACCTGCCT GCTCACATTC CCTACCAAGA GCTCAACAGT 
CAAGACAAGA GACGAAATTT CACCCTGGCC TTCCAAGCAG CAGAAAGTGT GGGCATCAAG 
TCCACTCTGG ACATCACTGA CATGGTCCAT ACAGAAAGAC CGGACTGGCA GAGTGTTATG 
ACTTACGTCA CCTCCATCTA CAAGTATTTT GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167234.1
CDS	236..3634
Translation	MKKAGRPVGNKAASGGGKGDSVTAGNCAGKNTVKSPTSAPLSKVKSNDDLLAAMAGSSAGTNNSVAKGKKSTSTHSTSCGTNTNNPDTKTKTTSGPSGKRTTSMTSKESNSSRERLRNSRNSSSKKQSSTGASDRAQPKHSRALAQTSDSESRMSKSKSDGQLSDKVALEAKVKNLLGLAKSKDVEILQLRGELRDMRVQLGLPEEDEEEERPPEREAAAVITAADVESTLLLLQEQNQAIRGELNLLKNENRMLKDRLNALGFSLEQRLDGADKTFGFPSTSPELSSGGAGAHGDCATVASSVEGSAPGSMEDLLTGGQRSGSTDNLDSESSEVYQAVTSSDDALDAPSGCGSSSSSESEGGPPACRSSSRKGSSGNTSEVSVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNGENERLGEEKVLLMDSLCQQSDKLEHCGRQIEYFRSLLDEHGVAYSVDEDIKSGRYMELEQRYVELAENARFEREQLLGVQQHLSNTLKMAEQDNAEAQNVIAALKERNHHMERLLDVERQERASMAAVLEECKAAVNNDQAELSRCRVLLEQERQKVAELYSIHNAGDKSDIHQLLEGVRLDKEEAEAKASKLQDDLGHARSEVACLQDTLNKLDAEYRDFQSVVQKELAEQKRAIEKQREDLQEKETEIGDMKETIFELEDEVEQHRAVKLHDNLIISDLENSMKKLQDQKHDMEREIKILHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLRRRLQEAQEKNEKLGKEIEEVKNRKQDEERGRVYNYMNAVERDLAALRQGMGLSRRPSTSSEPSPTVKTLIKSFDSASQGPGANATAVAAAAAAAAAAAAVTVTSTTPTAPLPRTPLSPSPMKTPPAAAVSPIQRHSITGSMAAAKPLSSLADKRPSYTDISMPAEHLLRAANSRPASALQRVSNMDTSKTITVSRRSSEEPKRDISTPDGGPASSLISMSSAAALSSSSSPTASVNPTARSRLREERKDPLSALAREYGGSKRNALLRWCQKKTEGYQNIDITNFSSSWNDGLAFCAVLHTYLPAHIPYQELNSQDKRRNFTLAFQAAESVGIKSTLDITDMVHTERPDWQSVMTYVTSIYKYFET

Target ORF information:

RefSeq Version	XM_005167177.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sperm antigen with calponin homology and coiled-coil domains 1-like a (specc1la), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167177.3

ORF Insert Sequence:

ATGAAGAAAG CAGGCAGGCC AGTGGGGAAT AAGGCAGCAA GTGGAGGAGG TAAAGGTGAC 
TCAGTGACGG CCGGAAACTG TGCTGGCAAA AACACTGTTA AGAGCCCTAC ATCAGCTCCT 
CTCTCCAAGG TGAAAAGCAA TGATGATCTT CTAGCTGCTA TGGCTGGTAG CAGTGCAGGC 
ACAAACAACA GTGTTGCCAA GGGCAAGAAG TCAACGTCTA CTCACTCAAC ATCTTGTGGA 
ACAAACACTA ATAACCCTGA CACCAAGACC AAAACCACCT CAGGTCCTTC TGGCAAACGG 
ACAACATCCA TGACCTCCAA GGAGTCGAAT TCCTCACGAG AGCGTTTAAG AAACTCTAGA 
AACTCCAGCA GTAAAAAGCA GTCGTCCACT GGTGCTTCAG ATAGAGCTCA GCCCAAGCAT 
TCCCGTGCAC TGGCCCAGAC CTCGGACTCG GAGAGCCGCA TGAGCAAATC AAAGTCTGAC 
GGACAGTTAA GCGATAAAGT GGCCCTGGAG GCCAAAGTGA AAAATCTGTT AGGTCTGGCC 
AAAAGCAAAG ACGTGGAAAT CTTGCAGCTC AGGGGTGAGC TGAGGGACAT GAGGGTTCAG 
CTGGGACTGC CTGAGGAAGA TGAGGAGGAA GAGAGGCCAC CTGAGAGAGA GGCGGCTGCA 
GTGATCACAG CTGCGGATGT GGAGTCAACA TTACTGCTCT TGCAGGAGCA GAATCAGGCC 
ATCCGTGGAG AGCTTAACCT GCTCAAAAAT GAAAACCGCA TGCTGAAGGA CCGCCTCAAT 
GCTCTGGGCT TTTCCCTAGA GCAGAGACTG GACGGGGCTG ATAAGACCTT TGGCTTTCCC 
TCCACCAGTC CTGAACTCTC CTCTGGAGGT GCTGGAGCTC ATGGAGACTG CGCCACGGTG 
GCCTCCTCCG TTGAGGGATC GGCACCAGGC TCTATGGAGG ACCTCCTGAC AGGAGGCCAG 
CGAAGTGGCT CAACGGACAA CTTAGACAGC GAGTCCAGTG AGGTCTACCA AGCAGTGACC 
TCCAGTGATG ATGCCTTGGA TGCTCCTTCA GGCTGCGGTT CTTCATCATC CTCTGAGTCT 
GAAGGTGGCC CTCCGGCCTG CCGCAGTTCC TCTCGTAAAG GAAGCAGTGG CAACACCAGT 
GAGGTGTCCG TGGCGTGCCT GACAGAGCGC ATTCATCAGA TGGAGGAGAA CCAACACAGC 
ACATCAGAAG AGCTCCAAGC CACTCTACAG GAACTGGCTG ACTTACAGCA GATCACACAG 
GAGCTAAACG GCGAAAATGA ACGCCTGGGA GAAGAAAAGG TCCTGCTTAT GGACTCTCTG 
TGTCAACAGA GTGACAAGCT AGAGCACTGT GGTCGTCAAA TAGAGTACTT TCGCTCACTC 
CTGGATGAGC ATGGTGTTGC CTACTCGGTA GATGAAGACA TCAAGAGTGG CCGATACATG 
GAGCTGGAGC AGCGCTACGT TGAGCTTGCG GAAAACGCCC GCTTTGAGCG TGAGCAGCTG 
TTAGGCGTGC AGCAGCATCT GAGTAACACT CTAAAGATGG CCGAACAGGA CAACGCTGAG 
GCTCAAAATG TGATCGCAGC ACTGAAGGAG CGCAACCATC ACATGGAGCG ACTTCTGGAC 
GTGGAAAGGC AAGAGCGAGC CAGCATGGCG GCAGTGCTGG AGGAGTGTAA AGCTGCGGTC 
AACAACGACC AGGCTGAGCT GAGTCGCTGT CGGGTCTTAC TCGAGCAGGA GAGACAAAAG 
GTTGCAGAAC TCTATTCCAT CCACAATGCT GGAGATAAGA GTGACATCCA CCAGCTCCTG 
GAGGGGGTGA GGCTGGACAA AGAGGAAGCT GAAGCCAAAG CCTCCAAACT ACAGGATGAC 
CTGGGCCATG CTCGCTCTGA GGTTGCATGT CTGCAGGACA CACTCAACAA GCTGGATGCA 
GAGTACAGGG ACTTCCAGAG TGTAGTGCAA AAAGAGCTGG CTGAGCAAAA GAGGGCTATA 
GAGAAGCAAC GGGAGGACCT GCAGGAGAAA GAGACTGAGA TCGGAGATAT GAAAGAGACC 
ATCTTTGAGT TGGAGGATGA AGTGGAGCAG CACCGAGCCG TCAAACTTCA TGACAACCTC 
ATCATCAGCG ACCTGGAGAA TTCGATGAAA AAGCTTCAGG ATCAGAAGCA TGACATGGAG 
AGAGAGATAA AGATCCTGCA CCGCAGACTC CGGGAAGAGT CTGCAGAGTG GCGTCAGTTT 
CAGGCAGATC TGCAAACTGC TGTGGTCATT GCTAATGACA TCAAATCAGA GGCTCAAGAG 
GAGATTGGTG ACTTACGGCG CCGCCTGCAA GAAGCCCAAG AGAAGAATGA GAAGTTGGGC 
AAAGAAATAG AGGAAGTTAA AAACCGCAAG CAAGATGAAG AGCGTGGTCG TGTGTATAAC 
TACATGAACG CTGTGGAGAG GGATCTAGCT GCGCTCCGGC AGGGTATGGG ATTGAGTCGA 
CGACCGTCCA CATCCTCCGA ACCATCGCCC ACCGTTAAAA CTCTCATCAA GAGCTTCGAC 
AGTGCCTCAC AGGGTCCTGG AGCTAACGCT ACTGCTGTCG CTGCCGCCGC CGCTGCTGCA 
GCAGCCGCTG CTGCTGTCAC CGTCACAAGC ACAACACCTA CAGCCCCTTT ACCCAGAACA 
CCTCTGAGTC CCAGCCCAAT GAAAACCCCA CCTGCTGCTG CCGTCTCACC CATACAGAGA 
CACTCCATCA CTGGCTCCAT GGCTGCCGCA AAGCCTCTCT CCTCACTTGC AGACAAAAGA 
CCCAGTTACA CAGACATCAG CATGCCAGCT GAGCATCTAC TCAGAGCAGC CAACAGTCGT 
CCAGCTTCAG CTCTTCAGAG AGTCTCAAAT ATGGATACAT CAAAGACCAT CACAGTATCT 
CGGAGAAGCA GTGAGGAGCC GAAGCGGGAT ATCAGCACTC CTGATGGAGG TCCGGCTTCC 
TCTCTGATAA GCATGAGCTC TGCTGCCGCG CTGTCCTCTT CTTCCTCTCC CACAGCCTCC 
GTCAACCCCA CTGCACGAAG CCGCCTTCGA GAGGAGCGAA AAGACCCTTT GTCAGCTTTG 
GCTCGAGAGT ATGGTGGTTC CAAAAGAAAT GCACTGTTGA GGTGGTGTCA GAAGAAGACT 
GAGGGTTATC AGAACATTGA CATTACTAAC TTCAGCAGCA GCTGGAATGA TGGACTTGCC 
TTCTGTGCTG TCCTTCACAC GTACCTGCCT GCTCACATTC CCTACCAAGA GCTCAACAGT 
CAAGACAAGA GACGAAATTT CACCCTGGCC TTCCAAGCAG CAGAAAGTGT GGGCATCAAG 
TCCACTCTGG ACATCACTGA CATGGTCCAT ACAGAAAGAC CGGACTGGCA GAGTGTTATG 
ACTTACGTCA CCTCCATCTA CAAGTATTTT GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

specc1la ( XM_009304055.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302330.1 Danio rerio sperm antigen with calponin homology and coiled-coil domains 1-like a (specc1la), transcript variant X2, mRNA.

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CloneID	ODa43589
Clone ID Related Accession (Same CDS sequence)	XM_009304055.2 , XM_009304055.3
Accession Version	XM_009304055.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	cytospin-A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304055.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG CAGGCAGGCC AGTGGGGAAT AAGGCAGCAA GTGGAGGAGG TAAAGGTGAC 
TCAGTGACGG CCGGAAACTG TGCTGGCAAA AACACTGTTA AGAGCCCTAC ATCAGCTCCT 
CTCTCCAAGG TGAAAAGCAA TGATGATCTT CTAGCTGCTA TGGCTGGTAG CAGTGCAGGC 
ACAAACAACA GTGTTGCCAA GGGCAAGAAG TCAACGTCTA CTCACTCAAC ATCTTGTGGA 
ACAAACACTA ATAACCCTGA CACCAAGACC AAAACCACCT CAGGTCCTTC TGGCAAACGG 
ACAACATCCA TGACCTCCAA GGAGTCGAAT TCCTCACGAG AGCGTTTAAG AAACTCTAGA 
AACTCCAGCA GTAAAAAGCA GTCGTCCACT GGTGCTTCAG ATAGAGCTCA GCCCAAGCAT 
TCCCGTGCAC TGGCCCAGAC CTCGGACTCG GAGAGCCGCA TGAGCAAATC AAAGTCTGAC 
GGACAGTTAA GCGATAAAGT GGCCCTGGAG GCCAAAGTGA AAAATCTGTT AGGTCTGGCC 
AAAAGCAAAG ACGTGGAAAT CTTGCAGCTC AGGGGTGAGC TGAGGGACAT GAGGGTTCAG 
CTGGGACTGC CTGAGGAAGA TGAGGAGGAA GAGAGGCCAC CTGAGAGAGA GGCGGCTGCA 
GTGATCACAG CTGCGGATGT GGAGTCAACA TTACTGCTCT TGCAGGAGCA GAATCAGGCC 
ATCCGTGGAG AGCTTAACCT GCTCAAAAAT GAAAACCGCA TGCTGAAGGA CCGCCTCAAT 
GCTCTGGGCT TTTCCCTAGA GCAGAGACTG GACGGGGCTG ATAAGACCTT TGGCTTTCCC 
TCCACCAGTC CTGAACTCTC CTCTGGAGGT GCTGGAGCTC ATGGAGACTG CGCCACGGTG 
GCCTCCTCCG TTGAGGGATC GGCACCAGGC TCTATGGAGG ACCTCCTGAC AGGAGGCCAG 
CGAAGTGGCT CAACGGACAA CTTAGACAGC GAGTCCAGTG AGGTCTACCA AGCAGTGACC 
TCCAGTGATG ATGCCTTGGA TGCTCCTTCA GGCTGCGGTT CTTCATCATC CTCTGAGTCT 
GAAGGTGGCC CTCCGGCCTG CCGCAGTTCC TCTCGTAAAG GAAGCAGTGG CAACACCAGT 
GAGGTGTCCG TGGCGTGCCT GACAGAGCGC ATTCATCAGA TGGAGGAGAA CCAACACAGC 
ACATCAGAAG AGCTCCAAGC CACTCTACAG GAACTGGCTG ACTTACAGCA GATCACACAG 
GAGCTAAACG GCGAAAATGA ACGCCTGGGA GAAGAAAAGG TCCTGCTTAT GGACTCTCTG 
TGTCAACAGA GTGACAAGCT AGAGCACTGT GGTCGTCAAA TAGAGTACTT TCGCTCACTC 
CTGGATGAGC ATGGTGTTGC CTACTCGGTA GATGAAGACA TCAAGAGTGG CCGATACATG 
GAGCTGGAGC AGCGCTACGT TGAGCTTGCG GAAAACGCCC GCTTTGAGCG TGAGCAGCTG 
TTAGGCGTGC AGCAGCATCT GAGTAACACT CTAAAGATGG CCGAACAGGA CAACGCTGAG 
GCTCAAAATG TGATCGCAGC ACTGAAGGAG CGCAACCATC ACATGGAGCG ACTTCTGGAC 
GTGGAAAGGC AAGAGCGAGC CAGCATGGCG GCAGTGCTGG AGGAGTGTAA AGCTGCGGTC 
AACAACGACC AGGCTGAGCT GAGTCGCTGT CGGGTCTTAC TCGAGCAGGA GAGACAAAAG 
GTTGCAGAAC TCTATTCCAT CCACAATGCT GGAGATAAGA GTGACATCCA CCAGCTCCTG 
GAGGGGGTGA GGCTGGACAA AGAGGAAGCT GAAGCCAAAG CCTCCAAACT ACAGGATGAC 
CTGGGCCATG CTCGCTCTGA GGTTGCATGT CTGCAGGACA CACTCAACAA GCTGGATGCA 
GAGTACAGGG ACTTCCAGAG TGTAGTGCAA AAAGAGCTGG CTGAGCAAAA GAGGGCTATA 
GAGAAGCAAC GGGAGGACCT GCAGGAGAAA GAGACTGAGA TCGGAGATAT GAAAGAGACC 
ATCTTTGAGT TGGAGGATGA AGTGGAGCAG CACCGAGCCG TCAAACTTCA TGACAACCTC 
ATCATCAGCG ACCTGGAGAA TTCGATGAAA AAGCTTCAGG ATCAGAAGCA TGACATGGAG 
AGAGAGATAA AGATCCTGCA CCGCAGACTC CGGGAAGAGT CTGCAGAGTG GCGTCAGTTT 
CAGGCAGATC TGCAAACTGC TGTGGTCATT GCTAATGACA TCAAATCAGA GGCTCAAGAG 
GAGATTGGTG ACTTACGGCG CCGCCTGCAA GAAGCCCAAG AGAAGAATGA GAAGTTGGGC 
AAAGAAATAG AGGAAGTTAA AAACCGCAAG CAAGATGAAG AGCGTGGTCG TGTGTATAAC 
TACATGAACG CTGTGGAGAG GGATCTAGCT GCGCTCCGGC AGGGTATGGG ATTGAGTCGA 
CGACCGTCCA CATCCTCCGA ACCATCGCCC ACCGTTAAAA CTCTCATCAA GAGCTTCGAC 
AGTGCCTCAC AGGGTCCTGG AGCTAACGCT ACTGCTGTCG CTGCCGCCGC CGCTGCTGCA 
GCAGCCGCTG CTGCTGTCAC CGTCACAAGC ACAACACCTA CAGCCCCTTT ACCCAGAACA 
CCTCTGAGTC CCAGCCCAAT GAAAACCCCA CCTGCTGCTG CCGTCTCACC CATACAGAGA 
CACTCCATCA CTGGCTCCAT GGCTGCCGCA AAGCCTCTCT CCTCACTTGC AGACAAAAGA 
CCCAGTTACA CAGACATCAG CATGCCAGCT GAGCATCTAC TCAGAGCAGC CAACAGTCGT 
CCAGCTTCAG CTCTTCAGAG AGTCTCAAAT ATGGATACAT CAAAGACCAT CACAGTATCT 
CGGAGAAGCA GTGAGGAGCC GAAGCGGGAT ATCAGCACTC CTGATGGAGG TCCGGCTTCC 
TCTCTGATAA GCATGAGCTC TGCTGCCGCG CTGTCCTCTT CTTCCTCTCC CACAGCCTCC 
GTCAACCCCA CTGCACGAAG CCGCCTTCGG TACAAAACCT GCCCTACAGC TCAAACTATC 
TCTTGCATAT TTCACATATT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302330.1
CDS	237..3320
Translation	MKKAGRPVGNKAASGGGKGDSVTAGNCAGKNTVKSPTSAPLSKVKSNDDLLAAMAGSSAGTNNSVAKGKKSTSTHSTSCGTNTNNPDTKTKTTSGPSGKRTTSMTSKESNSSRERLRNSRNSSSKKQSSTGASDRAQPKHSRALAQTSDSESRMSKSKSDGQLSDKVALEAKVKNLLGLAKSKDVEILQLRGELRDMRVQLGLPEEDEEEERPPEREAAAVITAADVESTLLLLQEQNQAIRGELNLLKNENRMLKDRLNALGFSLEQRLDGADKTFGFPSTSPELSSGGAGAHGDCATVASSVEGSAPGSMEDLLTGGQRSGSTDNLDSESSEVYQAVTSSDDALDAPSGCGSSSSSESEGGPPACRSSSRKGSSGNTSEVSVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNGENERLGEEKVLLMDSLCQQSDKLEHCGRQIEYFRSLLDEHGVAYSVDEDIKSGRYMELEQRYVELAENARFEREQLLGVQQHLSNTLKMAEQDNAEAQNVIAALKERNHHMERLLDVERQERASMAAVLEECKAAVNNDQAELSRCRVLLEQERQKVAELYSIHNAGDKSDIHQLLEGVRLDKEEAEAKASKLQDDLGHARSEVACLQDTLNKLDAEYRDFQSVVQKELAEQKRAIEKQREDLQEKETEIGDMKETIFELEDEVEQHRAVKLHDNLIISDLENSMKKLQDQKHDMEREIKILHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLRRRLQEAQEKNEKLGKEIEEVKNRKQDEERGRVYNYMNAVERDLAALRQGMGLSRRPSTSSEPSPTVKTLIKSFDSASQGPGANATAVAAAAAAAAAAAAVTVTSTTPTAPLPRTPLSPSPMKTPPAAAVSPIQRHSITGSMAAAKPLSSLADKRPSYTDISMPAEHLLRAANSRPASALQRVSNMDTSKTITVSRRSSEEPKRDISTPDGGPASSLISMSSAAALSSSSSPTASVNPTARSRLRYKTCPTAQTISCIFHIF

Target ORF information:

RefSeq Version	XM_009304055.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sperm antigen with calponin homology and coiled-coil domains 1-like a (specc1la), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304055.2

ORF Insert Sequence:

ATGAAGAAAG CAGGCAGGCC AGTGGGGAAT AAGGCAGCAA GTGGAGGAGG TAAAGGTGAC 
TCAGTGACGG CCGGAAACTG TGCTGGCAAA AACACTGTTA AGAGCCCTAC ATCAGCTCCT 
CTCTCCAAGG TGAAAAGCAA TGATGATCTT CTAGCTGCTA TGGCTGGTAG CAGTGCAGGC 
ACAAACAACA GTGTTGCCAA GGGCAAGAAG TCAACGTCTA CTCACTCAAC ATCTTGTGGA 
ACAAACACTA ATAACCCTGA CACCAAGACC AAAACCACCT CAGGTCCTTC TGGCAAACGG 
ACAACATCCA TGACCTCCAA GGAGTCGAAT TCCTCACGAG AGCGTTTAAG AAACTCTAGA 
AACTCCAGCA GTAAAAAGCA GTCGTCCACT GGTGCTTCAG ATAGAGCTCA GCCCAAGCAT 
TCCCGTGCAC TGGCCCAGAC CTCGGACTCG GAGAGCCGCA TGAGCAAATC AAAGTCTGAC 
GGACAGTTAA GCGATAAAGT GGCCCTGGAG GCCAAAGTGA AAAATCTGTT AGGTCTGGCC 
AAAAGCAAAG ACGTGGAAAT CTTGCAGCTC AGGGGTGAGC TGAGGGACAT GAGGGTTCAG 
CTGGGACTGC CTGAGGAAGA TGAGGAGGAA GAGAGGCCAC CTGAGAGAGA GGCGGCTGCA 
GTGATCACAG CTGCGGATGT GGAGTCAACA TTACTGCTCT TGCAGGAGCA GAATCAGGCC 
ATCCGTGGAG AGCTTAACCT GCTCAAAAAT GAAAACCGCA TGCTGAAGGA CCGCCTCAAT 
GCTCTGGGCT TTTCCCTAGA GCAGAGACTG GACGGGGCTG ATAAGACCTT TGGCTTTCCC 
TCCACCAGTC CTGAACTCTC CTCTGGAGGT GCTGGAGCTC ATGGAGACTG CGCCACGGTG 
GCCTCCTCCG TTGAGGGATC GGCACCAGGC TCTATGGAGG ACCTCCTGAC AGGAGGCCAG 
CGAAGTGGCT CAACGGACAA CTTAGACAGC GAGTCCAGTG AGGTCTACCA AGCAGTGACC 
TCCAGTGATG ATGCCTTGGA TGCTCCTTCA GGCTGCGGTT CTTCATCATC CTCTGAGTCT 
GAAGGTGGCC CTCCGGCCTG CCGCAGTTCC TCTCGTAAAG GAAGCAGTGG CAACACCAGT 
GAGGTGTCCG TGGCGTGCCT GACAGAGCGC ATTCATCAGA TGGAGGAGAA CCAACACAGC 
ACATCAGAAG AGCTCCAAGC CACTCTACAG GAACTGGCTG ACTTACAGCA GATCACACAG 
GAGCTAAACG GCGAAAATGA ACGCCTGGGA GAAGAAAAGG TCCTGCTTAT GGACTCTCTG 
TGTCAACAGA GTGACAAGCT AGAGCACTGT GGTCGTCAAA TAGAGTACTT TCGCTCACTC 
CTGGATGAGC ATGGTGTTGC CTACTCGGTA GATGAAGACA TCAAGAGTGG CCGATACATG 
GAGCTGGAGC AGCGCTACGT TGAGCTTGCG GAAAACGCCC GCTTTGAGCG TGAGCAGCTG 
TTAGGCGTGC AGCAGCATCT GAGTAACACT CTAAAGATGG CCGAACAGGA CAACGCTGAG 
GCTCAAAATG TGATCGCAGC ACTGAAGGAG CGCAACCATC ACATGGAGCG ACTTCTGGAC 
GTGGAAAGGC AAGAGCGAGC CAGCATGGCG GCAGTGCTGG AGGAGTGTAA AGCTGCGGTC 
AACAACGACC AGGCTGAGCT GAGTCGCTGT CGGGTCTTAC TCGAGCAGGA GAGACAAAAG 
GTTGCAGAAC TCTATTCCAT CCACAATGCT GGAGATAAGA GTGACATCCA CCAGCTCCTG 
GAGGGGGTGA GGCTGGACAA AGAGGAAGCT GAAGCCAAAG CCTCCAAACT ACAGGATGAC 
CTGGGCCATG CTCGCTCTGA GGTTGCATGT CTGCAGGACA CACTCAACAA GCTGGATGCA 
GAGTACAGGG ACTTCCAGAG TGTAGTGCAA AAAGAGCTGG CTGAGCAAAA GAGGGCTATA 
GAGAAGCAAC GGGAGGACCT GCAGGAGAAA GAGACTGAGA TCGGAGATAT GAAAGAGACC 
ATCTTTGAGT TGGAGGATGA AGTGGAGCAG CACCGAGCCG TCAAACTTCA TGACAACCTC 
ATCATCAGCG ACCTGGAGAA TTCGATGAAA AAGCTTCAGG ATCAGAAGCA TGACATGGAG 
AGAGAGATAA AGATCCTGCA CCGCAGACTC CGGGAAGAGT CTGCAGAGTG GCGTCAGTTT 
CAGGCAGATC TGCAAACTGC TGTGGTCATT GCTAATGACA TCAAATCAGA GGCTCAAGAG 
GAGATTGGTG ACTTACGGCG CCGCCTGCAA GAAGCCCAAG AGAAGAATGA GAAGTTGGGC 
AAAGAAATAG AGGAAGTTAA AAACCGCAAG CAAGATGAAG AGCGTGGTCG TGTGTATAAC 
TACATGAACG CTGTGGAGAG GGATCTAGCT GCGCTCCGGC AGGGTATGGG ATTGAGTCGA 
CGACCGTCCA CATCCTCCGA ACCATCGCCC ACCGTTAAAA CTCTCATCAA GAGCTTCGAC 
AGTGCCTCAC AGGGTCCTGG AGCTAACGCT ACTGCTGTCG CTGCCGCCGC CGCTGCTGCA 
GCAGCCGCTG CTGCTGTCAC CGTCACAAGC ACAACACCTA CAGCCCCTTT ACCCAGAACA 
CCTCTGAGTC CCAGCCCAAT GAAAACCCCA CCTGCTGCTG CCGTCTCACC CATACAGAGA 
CACTCCATCA CTGGCTCCAT GGCTGCCGCA AAGCCTCTCT CCTCACTTGC AGACAAAAGA 
CCCAGTTACA CAGACATCAG CATGCCAGCT GAGCATCTAC TCAGAGCAGC CAACAGTCGT 
CCAGCTTCAG CTCTTCAGAG AGTCTCAAAT ATGGATACAT CAAAGACCAT CACAGTATCT 
CGGAGAAGCA GTGAGGAGCC GAAGCGGGAT ATCAGCACTC CTGATGGAGG TCCGGCTTCC 
TCTCTGATAA GCATGAGCTC TGCTGCCGCG CTGTCCTCTT CTTCCTCTCC CACAGCCTCC 
GTCAACCCCA CTGCACGAAG CCGCCTTCGG TACAAAACCT GCCCTACAGC TCAAACTATC 
TCTTGCATAT TTCACATATT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

specc1la ( XM_009304055.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302330.1 Danio rerio sperm antigen with calponin homology and coiled-coil domains 1-like a (specc1la), transcript variant X2, mRNA.

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CloneID	ODa43589
Clone ID Related Accession (Same CDS sequence)	XM_009304055.2 , XM_009304055.3
Accession Version	XM_009304055.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cytospin-A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304055.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG CAGGCAGGCC AGTGGGGAAT AAGGCAGCAA GTGGAGGAGG TAAAGGTGAC 
TCAGTGACGG CCGGAAACTG TGCTGGCAAA AACACTGTTA AGAGCCCTAC ATCAGCTCCT 
CTCTCCAAGG TGAAAAGCAA TGATGATCTT CTAGCTGCTA TGGCTGGTAG CAGTGCAGGC 
ACAAACAACA GTGTTGCCAA GGGCAAGAAG TCAACGTCTA CTCACTCAAC ATCTTGTGGA 
ACAAACACTA ATAACCCTGA CACCAAGACC AAAACCACCT CAGGTCCTTC TGGCAAACGG 
ACAACATCCA TGACCTCCAA GGAGTCGAAT TCCTCACGAG AGCGTTTAAG AAACTCTAGA 
AACTCCAGCA GTAAAAAGCA GTCGTCCACT GGTGCTTCAG ATAGAGCTCA GCCCAAGCAT 
TCCCGTGCAC TGGCCCAGAC CTCGGACTCG GAGAGCCGCA TGAGCAAATC AAAGTCTGAC 
GGACAGTTAA GCGATAAAGT GGCCCTGGAG GCCAAAGTGA AAAATCTGTT AGGTCTGGCC 
AAAAGCAAAG ACGTGGAAAT CTTGCAGCTC AGGGGTGAGC TGAGGGACAT GAGGGTTCAG 
CTGGGACTGC CTGAGGAAGA TGAGGAGGAA GAGAGGCCAC CTGAGAGAGA GGCGGCTGCA 
GTGATCACAG CTGCGGATGT GGAGTCAACA TTACTGCTCT TGCAGGAGCA GAATCAGGCC 
ATCCGTGGAG AGCTTAACCT GCTCAAAAAT GAAAACCGCA TGCTGAAGGA CCGCCTCAAT 
GCTCTGGGCT TTTCCCTAGA GCAGAGACTG GACGGGGCTG ATAAGACCTT TGGCTTTCCC 
TCCACCAGTC CTGAACTCTC CTCTGGAGGT GCTGGAGCTC ATGGAGACTG CGCCACGGTG 
GCCTCCTCCG TTGAGGGATC GGCACCAGGC TCTATGGAGG ACCTCCTGAC AGGAGGCCAG 
CGAAGTGGCT CAACGGACAA CTTAGACAGC GAGTCCAGTG AGGTCTACCA AGCAGTGACC 
TCCAGTGATG ATGCCTTGGA TGCTCCTTCA GGCTGCGGTT CTTCATCATC CTCTGAGTCT 
GAAGGTGGCC CTCCGGCCTG CCGCAGTTCC TCTCGTAAAG GAAGCAGTGG CAACACCAGT 
GAGGTGTCCG TGGCGTGCCT GACAGAGCGC ATTCATCAGA TGGAGGAGAA CCAACACAGC 
ACATCAGAAG AGCTCCAAGC CACTCTACAG GAACTGGCTG ACTTACAGCA GATCACACAG 
GAGCTAAACG GCGAAAATGA ACGCCTGGGA GAAGAAAAGG TCCTGCTTAT GGACTCTCTG 
TGTCAACAGA GTGACAAGCT AGAGCACTGT GGTCGTCAAA TAGAGTACTT TCGCTCACTC 
CTGGATGAGC ATGGTGTTGC CTACTCGGTA GATGAAGACA TCAAGAGTGG CCGATACATG 
GAGCTGGAGC AGCGCTACGT TGAGCTTGCG GAAAACGCCC GCTTTGAGCG TGAGCAGCTG 
TTAGGCGTGC AGCAGCATCT GAGTAACACT CTAAAGATGG CCGAACAGGA CAACGCTGAG 
GCTCAAAATG TGATCGCAGC ACTGAAGGAG CGCAACCATC ACATGGAGCG ACTTCTGGAC 
GTGGAAAGGC AAGAGCGAGC CAGCATGGCG GCAGTGCTGG AGGAGTGTAA AGCTGCGGTC 
AACAACGACC AGGCTGAGCT GAGTCGCTGT CGGGTCTTAC TCGAGCAGGA GAGACAAAAG 
GTTGCAGAAC TCTATTCCAT CCACAATGCT GGAGATAAGA GTGACATCCA CCAGCTCCTG 
GAGGGGGTGA GGCTGGACAA AGAGGAAGCT GAAGCCAAAG CCTCCAAACT ACAGGATGAC 
CTGGGCCATG CTCGCTCTGA GGTTGCATGT CTGCAGGACA CACTCAACAA GCTGGATGCA 
GAGTACAGGG ACTTCCAGAG TGTAGTGCAA AAAGAGCTGG CTGAGCAAAA GAGGGCTATA 
GAGAAGCAAC GGGAGGACCT GCAGGAGAAA GAGACTGAGA TCGGAGATAT GAAAGAGACC 
ATCTTTGAGT TGGAGGATGA AGTGGAGCAG CACCGAGCCG TCAAACTTCA TGACAACCTC 
ATCATCAGCG ACCTGGAGAA TTCGATGAAA AAGCTTCAGG ATCAGAAGCA TGACATGGAG 
AGAGAGATAA AGATCCTGCA CCGCAGACTC CGGGAAGAGT CTGCAGAGTG GCGTCAGTTT 
CAGGCAGATC TGCAAACTGC TGTGGTCATT GCTAATGACA TCAAATCAGA GGCTCAAGAG 
GAGATTGGTG ACTTACGGCG CCGCCTGCAA GAAGCCCAAG AGAAGAATGA GAAGTTGGGC 
AAAGAAATAG AGGAAGTTAA AAACCGCAAG CAAGATGAAG AGCGTGGTCG TGTGTATAAC 
TACATGAACG CTGTGGAGAG GGATCTAGCT GCGCTCCGGC AGGGTATGGG ATTGAGTCGA 
CGACCGTCCA CATCCTCCGA ACCATCGCCC ACCGTTAAAA CTCTCATCAA GAGCTTCGAC 
AGTGCCTCAC AGGGTCCTGG AGCTAACGCT ACTGCTGTCG CTGCCGCCGC CGCTGCTGCA 
GCAGCCGCTG CTGCTGTCAC CGTCACAAGC ACAACACCTA CAGCCCCTTT ACCCAGAACA 
CCTCTGAGTC CCAGCCCAAT GAAAACCCCA CCTGCTGCTG CCGTCTCACC CATACAGAGA 
CACTCCATCA CTGGCTCCAT GGCTGCCGCA AAGCCTCTCT CCTCACTTGC AGACAAAAGA 
CCCAGTTACA CAGACATCAG CATGCCAGCT GAGCATCTAC TCAGAGCAGC CAACAGTCGT 
CCAGCTTCAG CTCTTCAGAG AGTCTCAAAT ATGGATACAT CAAAGACCAT CACAGTATCT 
CGGAGAAGCA GTGAGGAGCC GAAGCGGGAT ATCAGCACTC CTGATGGAGG TCCGGCTTCC 
TCTCTGATAA GCATGAGCTC TGCTGCCGCG CTGTCCTCTT CTTCCTCTCC CACAGCCTCC 
GTCAACCCCA CTGCACGAAG CCGCCTTCGG TACAAAACCT GCCCTACAGC TCAAACTATC 
TCTTGCATAT TTCACATATT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302330.1
CDS	230..3313
Translation	MKKAGRPVGNKAASGGGKGDSVTAGNCAGKNTVKSPTSAPLSKVKSNDDLLAAMAGSSAGTNNSVAKGKKSTSTHSTSCGTNTNNPDTKTKTTSGPSGKRTTSMTSKESNSSRERLRNSRNSSSKKQSSTGASDRAQPKHSRALAQTSDSESRMSKSKSDGQLSDKVALEAKVKNLLGLAKSKDVEILQLRGELRDMRVQLGLPEEDEEEERPPEREAAAVITAADVESTLLLLQEQNQAIRGELNLLKNENRMLKDRLNALGFSLEQRLDGADKTFGFPSTSPELSSGGAGAHGDCATVASSVEGSAPGSMEDLLTGGQRSGSTDNLDSESSEVYQAVTSSDDALDAPSGCGSSSSSESEGGPPACRSSSRKGSSGNTSEVSVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNGENERLGEEKVLLMDSLCQQSDKLEHCGRQIEYFRSLLDEHGVAYSVDEDIKSGRYMELEQRYVELAENARFEREQLLGVQQHLSNTLKMAEQDNAEAQNVIAALKERNHHMERLLDVERQERASMAAVLEECKAAVNNDQAELSRCRVLLEQERQKVAELYSIHNAGDKSDIHQLLEGVRLDKEEAEAKASKLQDDLGHARSEVACLQDTLNKLDAEYRDFQSVVQKELAEQKRAIEKQREDLQEKETEIGDMKETIFELEDEVEQHRAVKLHDNLIISDLENSMKKLQDQKHDMEREIKILHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLRRRLQEAQEKNEKLGKEIEEVKNRKQDEERGRVYNYMNAVERDLAALRQGMGLSRRPSTSSEPSPTVKTLIKSFDSASQGPGANATAVAAAAAAAAAAAAVTVTSTTPTAPLPRTPLSPSPMKTPPAAAVSPIQRHSITGSMAAAKPLSSLADKRPSYTDISMPAEHLLRAANSRPASALQRVSNMDTSKTITVSRRSSEEPKRDISTPDGGPASSLISMSSAAALSSSSSPTASVNPTARSRLRYKTCPTAQTISCIFHIF

Target ORF information:

RefSeq Version	XM_009304055.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sperm antigen with calponin homology and coiled-coil domains 1-like a (specc1la), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304055.3

ORF Insert Sequence:

ATGAAGAAAG CAGGCAGGCC AGTGGGGAAT AAGGCAGCAA GTGGAGGAGG TAAAGGTGAC 
TCAGTGACGG CCGGAAACTG TGCTGGCAAA AACACTGTTA AGAGCCCTAC ATCAGCTCCT 
CTCTCCAAGG TGAAAAGCAA TGATGATCTT CTAGCTGCTA TGGCTGGTAG CAGTGCAGGC 
ACAAACAACA GTGTTGCCAA GGGCAAGAAG TCAACGTCTA CTCACTCAAC ATCTTGTGGA 
ACAAACACTA ATAACCCTGA CACCAAGACC AAAACCACCT CAGGTCCTTC TGGCAAACGG 
ACAACATCCA TGACCTCCAA GGAGTCGAAT TCCTCACGAG AGCGTTTAAG AAACTCTAGA 
AACTCCAGCA GTAAAAAGCA GTCGTCCACT GGTGCTTCAG ATAGAGCTCA GCCCAAGCAT 
TCCCGTGCAC TGGCCCAGAC CTCGGACTCG GAGAGCCGCA TGAGCAAATC AAAGTCTGAC 
GGACAGTTAA GCGATAAAGT GGCCCTGGAG GCCAAAGTGA AAAATCTGTT AGGTCTGGCC 
AAAAGCAAAG ACGTGGAAAT CTTGCAGCTC AGGGGTGAGC TGAGGGACAT GAGGGTTCAG 
CTGGGACTGC CTGAGGAAGA TGAGGAGGAA GAGAGGCCAC CTGAGAGAGA GGCGGCTGCA 
GTGATCACAG CTGCGGATGT GGAGTCAACA TTACTGCTCT TGCAGGAGCA GAATCAGGCC 
ATCCGTGGAG AGCTTAACCT GCTCAAAAAT GAAAACCGCA TGCTGAAGGA CCGCCTCAAT 
GCTCTGGGCT TTTCCCTAGA GCAGAGACTG GACGGGGCTG ATAAGACCTT TGGCTTTCCC 
TCCACCAGTC CTGAACTCTC CTCTGGAGGT GCTGGAGCTC ATGGAGACTG CGCCACGGTG 
GCCTCCTCCG TTGAGGGATC GGCACCAGGC TCTATGGAGG ACCTCCTGAC AGGAGGCCAG 
CGAAGTGGCT CAACGGACAA CTTAGACAGC GAGTCCAGTG AGGTCTACCA AGCAGTGACC 
TCCAGTGATG ATGCCTTGGA TGCTCCTTCA GGCTGCGGTT CTTCATCATC CTCTGAGTCT 
GAAGGTGGCC CTCCGGCCTG CCGCAGTTCC TCTCGTAAAG GAAGCAGTGG CAACACCAGT 
GAGGTGTCCG TGGCGTGCCT GACAGAGCGC ATTCATCAGA TGGAGGAGAA CCAACACAGC 
ACATCAGAAG AGCTCCAAGC CACTCTACAG GAACTGGCTG ACTTACAGCA GATCACACAG 
GAGCTAAACG GCGAAAATGA ACGCCTGGGA GAAGAAAAGG TCCTGCTTAT GGACTCTCTG 
TGTCAACAGA GTGACAAGCT AGAGCACTGT GGTCGTCAAA TAGAGTACTT TCGCTCACTC 
CTGGATGAGC ATGGTGTTGC CTACTCGGTA GATGAAGACA TCAAGAGTGG CCGATACATG 
GAGCTGGAGC AGCGCTACGT TGAGCTTGCG GAAAACGCCC GCTTTGAGCG TGAGCAGCTG 
TTAGGCGTGC AGCAGCATCT GAGTAACACT CTAAAGATGG CCGAACAGGA CAACGCTGAG 
GCTCAAAATG TGATCGCAGC ACTGAAGGAG CGCAACCATC ACATGGAGCG ACTTCTGGAC 
GTGGAAAGGC AAGAGCGAGC CAGCATGGCG GCAGTGCTGG AGGAGTGTAA AGCTGCGGTC 
AACAACGACC AGGCTGAGCT GAGTCGCTGT CGGGTCTTAC TCGAGCAGGA GAGACAAAAG 
GTTGCAGAAC TCTATTCCAT CCACAATGCT GGAGATAAGA GTGACATCCA CCAGCTCCTG 
GAGGGGGTGA GGCTGGACAA AGAGGAAGCT GAAGCCAAAG CCTCCAAACT ACAGGATGAC 
CTGGGCCATG CTCGCTCTGA GGTTGCATGT CTGCAGGACA CACTCAACAA GCTGGATGCA 
GAGTACAGGG ACTTCCAGAG TGTAGTGCAA AAAGAGCTGG CTGAGCAAAA GAGGGCTATA 
GAGAAGCAAC GGGAGGACCT GCAGGAGAAA GAGACTGAGA TCGGAGATAT GAAAGAGACC 
ATCTTTGAGT TGGAGGATGA AGTGGAGCAG CACCGAGCCG TCAAACTTCA TGACAACCTC 
ATCATCAGCG ACCTGGAGAA TTCGATGAAA AAGCTTCAGG ATCAGAAGCA TGACATGGAG 
AGAGAGATAA AGATCCTGCA CCGCAGACTC CGGGAAGAGT CTGCAGAGTG GCGTCAGTTT 
CAGGCAGATC TGCAAACTGC TGTGGTCATT GCTAATGACA TCAAATCAGA GGCTCAAGAG 
GAGATTGGTG ACTTACGGCG CCGCCTGCAA GAAGCCCAAG AGAAGAATGA GAAGTTGGGC 
AAAGAAATAG AGGAAGTTAA AAACCGCAAG CAAGATGAAG AGCGTGGTCG TGTGTATAAC 
TACATGAACG CTGTGGAGAG GGATCTAGCT GCGCTCCGGC AGGGTATGGG ATTGAGTCGA 
CGACCGTCCA CATCCTCCGA ACCATCGCCC ACCGTTAAAA CTCTCATCAA GAGCTTCGAC 
AGTGCCTCAC AGGGTCCTGG AGCTAACGCT ACTGCTGTCG CTGCCGCCGC CGCTGCTGCA 
GCAGCCGCTG CTGCTGTCAC CGTCACAAGC ACAACACCTA CAGCCCCTTT ACCCAGAACA 
CCTCTGAGTC CCAGCCCAAT GAAAACCCCA CCTGCTGCTG CCGTCTCACC CATACAGAGA 
CACTCCATCA CTGGCTCCAT GGCTGCCGCA AAGCCTCTCT CCTCACTTGC AGACAAAAGA 
CCCAGTTACA CAGACATCAG CATGCCAGCT GAGCATCTAC TCAGAGCAGC CAACAGTCGT 
CCAGCTTCAG CTCTTCAGAG AGTCTCAAAT ATGGATACAT CAAAGACCAT CACAGTATCT 
CGGAGAAGCA GTGAGGAGCC GAAGCGGGAT ATCAGCACTC CTGATGGAGG TCCGGCTTCC 
TCTCTGATAA GCATGAGCTC TGCTGCCGCG CTGTCCTCTT CTTCCTCTCC CACAGCCTCC 
GTCAACCCCA CTGCACGAAG CCGCCTTCGG TACAAAACCT GCCCTACAGC TCAAACTATC 
TCTTGCATAT TTCACATATT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional analysis of SPECC1L in craniofacial development and oblique facial cleft pathogenesis.
Plastic and reconstructive surgery134(4)748-59(2014 Oct)
Gfrerer L,Shubinets V,Hoyos T,Kong Y,Nguyen C,Pietschmann P,Morton CC,Maas RL,Liao EC

Deficiency of the cytoskeletal protein SPECC1L leads to oblique facial clefting.
American journal of human genetics89(1)44-55(2011 Jul)
Saadi I,Alkuraya FS,Gisselbrecht SS,Goessling W,Cavallesco R,Turbe-Doan A,Petrin AL,Harris J,Siddiqui U,Grix AW Jr,Hove HD,Leboulch P,Glover TW,Morton CC,Richieri-Costa A,Murray JC,Erickson RP,Maas RL

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specc1la cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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