SPECC1L cDNA ORF clone, Bos taurus(cattle)

The following SPECC1L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPECC1L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa28164 XM_005218229.4
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa93287 XM_015475619.2
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa93285 XM_015475617.2
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa93286 XM_015475618.2
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa28162 NM_001205864.1
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa28162 XM_005218226.4
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa28162 XM_005218227.3
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa28163 XM_005218228.4
Latest version!
Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa28162 XM_005218227.2 Bos taurus sperm antigen with calponin homology and coiled-coil domains 1-like (SPECC1L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa93285 XM_015475617.1 Bos taurus sperm antigen with calponin homology and coiled-coil domains 1-like (SPECC1L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa93287 XM_015475619.1 Bos taurus sperm antigen with calponin homology and coiled-coil domains 1-like (SPECC1L), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa28164 XM_005218229.3 Bos taurus sperm antigen with calponin homology and coiled-coil domains 1-like (SPECC1L), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa28162 XM_005218226.3 Bos taurus sperm antigen with calponin homology and coiled-coil domains 1-like (SPECC1L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa28163 XM_005218228.3 Bos taurus sperm antigen with calponin homology and coiled-coil domains 1-like (SPECC1L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa93286 XM_015475618.1 Bos taurus sperm antigen with calponin homology and coiled-coil domains 1-like (SPECC1L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa28164
    Clone ID Related Accession (Same CDS sequence) XM_005218229.3 , XM_005218229.4
    Accession Version XM_005218229.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104461.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218229.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAAAGAG AAATCAAGAC CCTGCACAGG CGACTGCGGG AGGAATCAGC AGAATGGCGG 
    CAGTTCCAGG CTGATCTGCA GACCGCGGTG GTCATCGCTA ATGACATCAA GTCTGAAGCC
    CAAGAGGAGA TTGGTGACCT GAAGCGCCGC TTACATGAAG CTCAAGAGAA AAACGAGAAG
    CTCACGAAAG AATTGGAGGA AATAAAGTCA CGCAAGCAGG AGGAGGAGCG TGGCCGGGTG
    TATAACTACA TGAATGCTGT GGAGAGAGAC CTGGCGGCCC TGAGGCAGGG CATGGGGCTG
    AGCAGAAGGT CCTCCACGTC CTCGGAGCCG ACGCCCACCG TGAAGACCCT CATCAAGTCC
    TTCGACAGCG CATCTCAAGT GCCCAGCCCT GCCGCGGCAG CGATGCCCCG GACGCCTCTG
    AGCCCAAGCC CTATGAAGAC GCCCCCTGCG GCCGCCGTCT CCCCCATGCA GAGACATTCC
    ATCAGTGGAC CCATCTCCAC GTCCAAACCC CTGACAGCCC TCTCAGATAA GAGACCAAAC
    TACGGGGAGA TCCCTGTGCA AGAGCATCTG CTGAGAACGT CTTCGACCAG CCGACCTGCT
    TCTCTGCCGA GAGTGCCTGC CATGGAGAGC GCAAAGACCC TCTCAGTGTC CAGACGAAGC
    AGCGAGGAGA TGAAGCGCGA CGTGTCTGCT CCCGAGGGCA CGTCGCCCGC CGCGCTCATG
    GCCATGGGGG CCACCTCGCC GCAGCTGTCG CTCTCCTCCT CGCCCACGGC CTCCGTGACC
    CCCACCGCCC GGAGCCGCAT CAGGGAAGAA AGGAAGGATC CTCTGTCGGC CTTGGCGAGA
    GAATATGGGG GTTCCAAGAG GAACGCCTTG CTGAAGTGGT GTCAGAAGAA AACCGAGGGC
    TACCAGAACA TTGACATCAC CAACTTCAGC AGCAGCTGGA ACGATGGGCT GGCCTTCTGC
    GCCCTCCTGC ACACCTACCT CCCCGCACAC ATCCCCTACC AGGAGCTCAA CAGCCAGGAC
    AAGCGGAGGA ACTTCACGCT GGCCTTCCAG GCGGCCGAAA GCGTTGGCAT CAAGTCCACA
    CTGGACATCA ACGAGATGGT GCGGACCGAG CGGCCCGACT GGCAGAACGT GATGCTCTAC
    GTGACCGCCA TCTACAAATA CTTCGAGACC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218286.1
    CDS1409..2581
    Translation

    Target ORF information:

    RefSeq Version XM_005218229.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1-like (SPECC1L), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218229.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAAAGAG AAATCAAGAC CCTGCACAGG CGACTGCGGG AGGAATCAGC AGAATGGCGG 
    CAGTTCCAGG CTGATCTGCA GACCGCGGTG GTCATCGCTA ATGACATCAA GTCTGAAGCC
    CAAGAGGAGA TTGGTGACCT GAAGCGCCGC TTACATGAAG CTCAAGAGAA AAACGAGAAG
    CTCACGAAAG AATTGGAGGA AATAAAGTCA CGCAAGCAGG AGGAGGAGCG TGGCCGGGTG
    TATAACTACA TGAATGCTGT GGAGAGAGAC CTGGCGGCCC TGAGGCAGGG CATGGGGCTG
    AGCAGAAGGT CCTCCACGTC CTCGGAGCCG ACGCCCACCG TGAAGACCCT CATCAAGTCC
    TTCGACAGCG CATCTCAAGT GCCCAGCCCT GCCGCGGCAG CGATGCCCCG GACGCCTCTG
    AGCCCAAGCC CTATGAAGAC GCCCCCTGCG GCCGCCGTCT CCCCCATGCA GAGACATTCC
    ATCAGTGGAC CCATCTCCAC GTCCAAACCC CTGACAGCCC TCTCAGATAA GAGACCAAAC
    TACGGGGAGA TCCCTGTGCA AGAGCATCTG CTGAGAACGT CTTCGACCAG CCGACCTGCT
    TCTCTGCCGA GAGTGCCTGC CATGGAGAGC GCAAAGACCC TCTCAGTGTC CAGACGAAGC
    AGCGAGGAGA TGAAGCGCGA CGTGTCTGCT CCCGAGGGCA CGTCGCCCGC CGCGCTCATG
    GCCATGGGGG CCACCTCGCC GCAGCTGTCG CTCTCCTCCT CGCCCACGGC CTCCGTGACC
    CCCACCGCCC GGAGCCGCAT CAGGGAAGAA AGGAAGGATC CTCTGTCGGC CTTGGCGAGA
    GAATATGGGG GTTCCAAGAG GAACGCCTTG CTGAAGTGGT GTCAGAAGAA AACCGAGGGC
    TACCAGAACA TTGACATCAC CAACTTCAGC AGCAGCTGGA ACGATGGGCT GGCCTTCTGC
    GCCCTCCTGC ACACCTACCT CCCCGCACAC ATCCCCTACC AGGAGCTCAA CAGCCAGGAC
    AAGCGGAGGA ACTTCACGCT GGCCTTCCAG GCGGCCGAAA GCGTTGGCAT CAAGTCCACA
    CTGGACATCA ACGAGATGGT GCGGACCGAG CGGCCCGACT GGCAGAACGT GATGCTCTAC
    GTGACCGCCA TCTACAAATA CTTCGAGACC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28164
    Clone ID Related Accession (Same CDS sequence) XM_005218229.3 , XM_005218229.4
    Accession Version XM_005218229.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218229.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAAAGAG AAATCAAGAC CCTGCACAGG CGACTGCGGG AGGAATCAGC AGAATGGCGG 
    CAGTTCCAGG CTGATCTGCA GACCGCGGTG GTCATCGCTA ATGACATCAA GTCTGAAGCC
    CAAGAGGAGA TTGGTGACCT GAAGCGCCGC TTACATGAAG CTCAAGAGAA AAACGAGAAG
    CTCACGAAAG AATTGGAGGA AATAAAGTCA CGCAAGCAGG AGGAGGAGCG TGGCCGGGTG
    TATAACTACA TGAATGCTGT GGAGAGAGAC CTGGCGGCCC TGAGGCAGGG CATGGGGCTG
    AGCAGAAGGT CCTCCACGTC CTCGGAGCCG ACGCCCACCG TGAAGACCCT CATCAAGTCC
    TTCGACAGCG CATCTCAAGT GCCCAGCCCT GCCGCGGCAG CGATGCCCCG GACGCCTCTG
    AGCCCAAGCC CTATGAAGAC GCCCCCTGCG GCCGCCGTCT CCCCCATGCA GAGACATTCC
    ATCAGTGGAC CCATCTCCAC GTCCAAACCC CTGACAGCCC TCTCAGATAA GAGACCAAAC
    TACGGGGAGA TCCCTGTGCA AGAGCATCTG CTGAGAACGT CTTCGACCAG CCGACCTGCT
    TCTCTGCCGA GAGTGCCTGC CATGGAGAGC GCAAAGACCC TCTCAGTGTC CAGACGAAGC
    AGCGAGGAGA TGAAGCGCGA CGTGTCTGCT CCCGAGGGCA CGTCGCCCGC CGCGCTCATG
    GCCATGGGGG CCACCTCGCC GCAGCTGTCG CTCTCCTCCT CGCCCACGGC CTCCGTGACC
    CCCACCGCCC GGAGCCGCAT CAGGGAAGAA AGGAAGGATC CTCTGTCGGC CTTGGCGAGA
    GAATATGGGG GTTCCAAGAG GAACGCCTTG CTGAAGTGGT GTCAGAAGAA AACCGAGGGC
    TACCAGAACA TTGACATCAC CAACTTCAGC AGCAGCTGGA ACGATGGGCT GGCCTTCTGC
    GCCCTCCTGC ACACCTACCT CCCCGCACAC ATCCCCTACC AGGAGCTCAA CAGCCAGGAC
    AAGCGGAGGA ACTTCACGCT GGCCTTCCAG GCGGCCGAAA GCGTTGGCAT CAAGTCCACA
    CTGGACATCA ACGAGATGGT GCGGACCGAG CGGCCCGACT GGCAGAACGT GATGCTCTAC
    GTGACCGCCA TCTACAAATA CTTCGAGACC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218286.1
    CDS1411..2583
    Translation

    Target ORF information:

    RefSeq Version XM_005218229.4
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218229.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAAAGAG AAATCAAGAC CCTGCACAGG CGACTGCGGG AGGAATCAGC AGAATGGCGG 
    CAGTTCCAGG CTGATCTGCA GACCGCGGTG GTCATCGCTA ATGACATCAA GTCTGAAGCC
    CAAGAGGAGA TTGGTGACCT GAAGCGCCGC TTACATGAAG CTCAAGAGAA AAACGAGAAG
    CTCACGAAAG AATTGGAGGA AATAAAGTCA CGCAAGCAGG AGGAGGAGCG TGGCCGGGTG
    TATAACTACA TGAATGCTGT GGAGAGAGAC CTGGCGGCCC TGAGGCAGGG CATGGGGCTG
    AGCAGAAGGT CCTCCACGTC CTCGGAGCCG ACGCCCACCG TGAAGACCCT CATCAAGTCC
    TTCGACAGCG CATCTCAAGT GCCCAGCCCT GCCGCGGCAG CGATGCCCCG GACGCCTCTG
    AGCCCAAGCC CTATGAAGAC GCCCCCTGCG GCCGCCGTCT CCCCCATGCA GAGACATTCC
    ATCAGTGGAC CCATCTCCAC GTCCAAACCC CTGACAGCCC TCTCAGATAA GAGACCAAAC
    TACGGGGAGA TCCCTGTGCA AGAGCATCTG CTGAGAACGT CTTCGACCAG CCGACCTGCT
    TCTCTGCCGA GAGTGCCTGC CATGGAGAGC GCAAAGACCC TCTCAGTGTC CAGACGAAGC
    AGCGAGGAGA TGAAGCGCGA CGTGTCTGCT CCCGAGGGCA CGTCGCCCGC CGCGCTCATG
    GCCATGGGGG CCACCTCGCC GCAGCTGTCG CTCTCCTCCT CGCCCACGGC CTCCGTGACC
    CCCACCGCCC GGAGCCGCAT CAGGGAAGAA AGGAAGGATC CTCTGTCGGC CTTGGCGAGA
    GAATATGGGG GTTCCAAGAG GAACGCCTTG CTGAAGTGGT GTCAGAAGAA AACCGAGGGC
    TACCAGAACA TTGACATCAC CAACTTCAGC AGCAGCTGGA ACGATGGGCT GGCCTTCTGC
    GCCCTCCTGC ACACCTACCT CCCCGCACAC ATCCCCTACC AGGAGCTCAA CAGCCAGGAC
    AAGCGGAGGA ACTTCACGCT GGCCTTCCAG GCGGCCGAAA GCGTTGGCAT CAAGTCCACA
    CTGGACATCA ACGAGATGGT GCGGACCGAG CGGCCCGACT GGCAGAACGT GATGCTCTAC
    GTGACCGCCA TCTACAAATA CTTCGAGACC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93287
    Clone ID Related Accession (Same CDS sequence) XM_015475619.1 , XM_015475619.2
    Accession Version XM_015475619.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2820bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104461.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGG GAAGAAAGGA
    AGGATCCTCT GTCGGCCTTG GCGAGAGAAT ATGGGGGTTC CAAGAGGAAC GCCTTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331105.1
    CDS165..2984
    Translation

    Target ORF information:

    RefSeq Version XM_015475619.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1-like (SPECC1L), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475619.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGG GAAGAAAGGA
    AGGATCCTCT GTCGGCCTTG GCGAGAGAAT ATGGGGGTTC CAAGAGGAAC GCCTTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93287
    Clone ID Related Accession (Same CDS sequence) XM_015475619.1 , XM_015475619.2
    Accession Version XM_015475619.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2820bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475619.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGG GAAGAAAGGA
    AGGATCCTCT GTCGGCCTTG GCGAGAGAAT ATGGGGGTTC CAAGAGGAAC GCCTTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331105.1
    CDS185..3004
    Translation

    Target ORF information:

    RefSeq Version XM_015475619.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475619.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGG GAAGAAAGGA
    AGGATCCTCT GTCGGCCTTG GCGAGAGAAT ATGGGGGTTC CAAGAGGAAC GCCTTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93285
    Clone ID Related Accession (Same CDS sequence) XM_015475617.2 , XM_015475617.1
    Accession Version XM_015475617.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475617.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTGGAG GGGTGACCGT GACCAATGGT GTCAAAGGCA AGAAGAACGC CTGCTCCTCG 
    GCCGCGCCAG CCGCGCCCGC GCCTGCCGTG AGCGCCACGG AGAACAGGCC CAGGACCGGC
    ACAGGCACGA GTTCTTCAAC CAAGCGGAGC ACTTGCACAG GTAATAAAGA GCCCAGTTCT
    ACTAGAGAAA GATTGCGTGA GCGCACCCGA CTAAACCAGA GCAAAAAACT ACCTTCTGCA
    GGTCAGGGGG CTAATGACGT GGCGTCGGCC AAACGCTCCC GCAGTCGCAC GGCTGCGGAA
    TGCGACGTCC GCATGAGCAA GTCCAAGTCG GACAATCAGA TCAGTGACAA AGCTGCTCTG
    GAGGCCAGAG TGAAGGATCT TCTCACCTTG GCCAAAACCA AGGACGTGGA GATTTTACAT
    CTGCGGAGCG AGCTCCGGGA CATGCGTGCT CAGCTGGGTA TCAGCGAGGA CCCCCTCACC
    GAGGGCGCCG AGACGTCCGA GGAGAAGGAA GCCAGCACCG TGCACCAGCC AACCGACGTG
    GAGTCCACCT TGCTGCAGCT GCAGGAGCAG AACGCCGCCA TCCGCGAAGA GCTCAACCAG
    CTGAAGAACG AGAACCGGAT GCTGAAGGAC CGGCTGAACG CGCTGGGCTT CTCCCTGGAG
    CAGAGGCTGG ACCATTCCGA AAAGCTGTTT GGCTATCAGT CCCTGAGCCC AGAGATCACC
    CCTGGTAACC AGAGCGACGG CGGCGGGACG CTGACCTCCT CGGTGGAGGG CTCGGCCCCT
    GGCTCAGTGG AGGATCTGCT GAGCCAGGAT GAAAACACGC TCATGGACCA TCAGCACAGC
    AACTCCCTGG ACAACCTGGA CAGTGAGTGC AGTGAGGTGT ACCAGCCCCT CACGTCCAGC
    GACGACGCCC TGGACGCGCC CTCGTCCTCG GAGTCAGAAG GCCTTCCGAG CGTAGAGCGC
    TCTCGCAAGG GCAGCAGCGG GAATGCCAGC GAAGTGTCTG TCGCGTGCCT GACGGAGCGG
    ATACACCAGA TGGAGGAGAA CCAGCACAGC ACGAGCGAGG AGCTGCAGGC CACTCTGCAG
    GAGCTGGCTG ACCTGCAGCA GATCACCCAG GAGCTGAACA GCGAGAATGA GCGGCTCGGC
    GAGGAGAAGG TGATTCTCAT GGAGTCCCTG TGTCAGCAGA GCGACAAGCT GGAGCACTTC
    AGCCGGCAGA TTGAGTATTT CCGCTCCCTC CTGGACGAGC ATCACATTTC CTATGTCATC
    GACGAAGACG TGAAAAGCGG GCGCTACATG GAGCTGGAGC AGCGCTACAT GGACCTCGCT
    GAGAACAGCC GCTTTGAACG GGAGCAGCTC CTTGGCGTCC AGCAGCACCT AAGCAACACC
    CTGAAGATGG CCGAGCAGGA CAACAAGGAG GCCCAGGAGC TGATCGGCGC GCTCAAGGAG
    CGCAGCCACC ACATGGAGCG GGCCGTCGAG TCCGAGCAGA AGGGCAAGGC GGCCTTGGCC
    GCCACCCTGG AGGAGCTCAG AGCCACCCTG GCCAGTGACC AGATCGAGAT GAATCGCCTG
    AAGGCCCAGC TGGAGAACGA GAAGCAGAAA GTGGCGGAGC TGTACTCTAT CCATAACTCT
    GGAGACAAGT CTGACATTCA GGACCTCCTG GAGAGCGTCA GGCTGGACAA GGAGAAAGCA
    GAGACTCTGG CCAGTAGCCT GCAGGAAGAC CTGGCCCACA CCCGGAATGA TGCCAACCGG
    CTGCAGGACG CCATCGCAAA GGTGGAAGAC GAGTACCGAG CCTTCCAAGG GGAAGCTAAG
    AAGCAGATAG AGGACCTGAA CGTGGCCGTA GAGAAGCTAC GCTCAGAGCT GGAAGAAAGA
    GACACAGAGA GGAGTGACAT GAAGGAAACG ATCTTTGAGC TTGAGGATGA AGTGGAGCAG
    CACCGGGCAG TGAAGCTGCA CGACAACCTC ATCATCTCGG ACTTGGAGAA TACGGTTAAA
    AAACTCCAGG ACCAAAAACA TGACATGGAA AGAGAAATCA AGACCCTGCA CAGGCGACTG
    CGGGAGGAAT CAGCAGAATG GCGGCAGTTC CAGGCTGATC TGCAGACCGC GGTGGTCATC
    GCTAATGACA TCAAGTCTGA AGCCCAAGAG GAGATTGGTG ACCTGAAGCG CCGCTTACAT
    GAAGCTCAAG AGAAAAACGA GAAGCTCACG AAAGAATTGG AGGAAATAAA GTCACGCAAG
    CAGGAGGAGG AGCGTGGCCG GGTGTATAAC TACATGAATG CTGTGGAGAG AGACCTGGCG
    GCCCTGAGGC AGGGCATGGG GCTGAGCAGA AGGTCCTCCA CGTCCTCGGA GCCGACGCCC
    ACCGTGAAGA CCCTCATCAA GTCCTTCGAC AGCGCATCTC AAGTGCCCAG CCCTGCCGCG
    GCAGCGATGC CCCGGACGCC TCTGAGCCCA AGCCCTATGA AGACGCCCCC TGCGGCCGCC
    GTCTCCCCCA TGCAGAGACA TTCCATCAGT GGACCCATCT CCACGTCCAA ACCCCTGACA
    GCCCTCTCAG ATAAGAGACC AAACTACGGG GAGATCCCTG TGCAAGAGCA TCTGCTGAGA
    ACGTCTTCGA CCAGCCGACC TGCTTCTCTG CCGAGAGTGC CTGCCATGGA GAGCGCAAAG
    ACCCTCTCAG TGTCCAGACG AAGCAGCGAG GAGATGAAGC GCGACGTGTC TGCTCCCGAG
    GGCACGTCGC CCGCCGCGCT CATGGCCATG GGGGCCACCT CGCCGCAGCT GTCGCTCTCC
    TCCTCGCCCA CGGCCTCCGT GACCCCCACC GCCCGGAGCC GCATCAGGGA AGAAAGGAAG
    GATCCTCTGT CGGCCTTGGC GAGAGAATAT GGGGGTTCCA AGAGGAACGC CTTGCTGAAG
    TGGTGTCAGA AGAAAACCGA GGGCTACCAG AACATTGACA TCACCAACTT CAGCAGCAGC
    TGGAACGATG GGCTGGCCTT CTGCGCCCTC CTGCACACCT ACCTCCCCGC ACACATCCCC
    TACCAGGAGC TCAACAGCCA GGACAAGCGG AGGAACTTCA CGCTGGCCTT CCAGGCGGCC
    GAAAGCGTTG GCATCAAGTC CACACTGGAC ATCAACGAGA TGGTGCGGAC CGAGCGGCCC
    GACTGGCAGA ACGTGATGCT CTACGTGACC GCCATCTACA AATACTTCGA GACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331103.1
    CDS225..3401
    Translation

    Target ORF information:

    RefSeq Version XM_015475617.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475617.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTGGAG GGGTGACCGT GACCAATGGT GTCAAAGGCA AGAAGAACGC CTGCTCCTCG 
    GCCGCGCCAG CCGCGCCCGC GCCTGCCGTG AGCGCCACGG AGAACAGGCC CAGGACCGGC
    ACAGGCACGA GTTCTTCAAC CAAGCGGAGC ACTTGCACAG GTAATAAAGA GCCCAGTTCT
    ACTAGAGAAA GATTGCGTGA GCGCACCCGA CTAAACCAGA GCAAAAAACT ACCTTCTGCA
    GGTCAGGGGG CTAATGACGT GGCGTCGGCC AAACGCTCCC GCAGTCGCAC GGCTGCGGAA
    TGCGACGTCC GCATGAGCAA GTCCAAGTCG GACAATCAGA TCAGTGACAA AGCTGCTCTG
    GAGGCCAGAG TGAAGGATCT TCTCACCTTG GCCAAAACCA AGGACGTGGA GATTTTACAT
    CTGCGGAGCG AGCTCCGGGA CATGCGTGCT CAGCTGGGTA TCAGCGAGGA CCCCCTCACC
    GAGGGCGCCG AGACGTCCGA GGAGAAGGAA GCCAGCACCG TGCACCAGCC AACCGACGTG
    GAGTCCACCT TGCTGCAGCT GCAGGAGCAG AACGCCGCCA TCCGCGAAGA GCTCAACCAG
    CTGAAGAACG AGAACCGGAT GCTGAAGGAC CGGCTGAACG CGCTGGGCTT CTCCCTGGAG
    CAGAGGCTGG ACCATTCCGA AAAGCTGTTT GGCTATCAGT CCCTGAGCCC AGAGATCACC
    CCTGGTAACC AGAGCGACGG CGGCGGGACG CTGACCTCCT CGGTGGAGGG CTCGGCCCCT
    GGCTCAGTGG AGGATCTGCT GAGCCAGGAT GAAAACACGC TCATGGACCA TCAGCACAGC
    AACTCCCTGG ACAACCTGGA CAGTGAGTGC AGTGAGGTGT ACCAGCCCCT CACGTCCAGC
    GACGACGCCC TGGACGCGCC CTCGTCCTCG GAGTCAGAAG GCCTTCCGAG CGTAGAGCGC
    TCTCGCAAGG GCAGCAGCGG GAATGCCAGC GAAGTGTCTG TCGCGTGCCT GACGGAGCGG
    ATACACCAGA TGGAGGAGAA CCAGCACAGC ACGAGCGAGG AGCTGCAGGC CACTCTGCAG
    GAGCTGGCTG ACCTGCAGCA GATCACCCAG GAGCTGAACA GCGAGAATGA GCGGCTCGGC
    GAGGAGAAGG TGATTCTCAT GGAGTCCCTG TGTCAGCAGA GCGACAAGCT GGAGCACTTC
    AGCCGGCAGA TTGAGTATTT CCGCTCCCTC CTGGACGAGC ATCACATTTC CTATGTCATC
    GACGAAGACG TGAAAAGCGG GCGCTACATG GAGCTGGAGC AGCGCTACAT GGACCTCGCT
    GAGAACAGCC GCTTTGAACG GGAGCAGCTC CTTGGCGTCC AGCAGCACCT AAGCAACACC
    CTGAAGATGG CCGAGCAGGA CAACAAGGAG GCCCAGGAGC TGATCGGCGC GCTCAAGGAG
    CGCAGCCACC ACATGGAGCG GGCCGTCGAG TCCGAGCAGA AGGGCAAGGC GGCCTTGGCC
    GCCACCCTGG AGGAGCTCAG AGCCACCCTG GCCAGTGACC AGATCGAGAT GAATCGCCTG
    AAGGCCCAGC TGGAGAACGA GAAGCAGAAA GTGGCGGAGC TGTACTCTAT CCATAACTCT
    GGAGACAAGT CTGACATTCA GGACCTCCTG GAGAGCGTCA GGCTGGACAA GGAGAAAGCA
    GAGACTCTGG CCAGTAGCCT GCAGGAAGAC CTGGCCCACA CCCGGAATGA TGCCAACCGG
    CTGCAGGACG CCATCGCAAA GGTGGAAGAC GAGTACCGAG CCTTCCAAGG GGAAGCTAAG
    AAGCAGATAG AGGACCTGAA CGTGGCCGTA GAGAAGCTAC GCTCAGAGCT GGAAGAAAGA
    GACACAGAGA GGAGTGACAT GAAGGAAACG ATCTTTGAGC TTGAGGATGA AGTGGAGCAG
    CACCGGGCAG TGAAGCTGCA CGACAACCTC ATCATCTCGG ACTTGGAGAA TACGGTTAAA
    AAACTCCAGG ACCAAAAACA TGACATGGAA AGAGAAATCA AGACCCTGCA CAGGCGACTG
    CGGGAGGAAT CAGCAGAATG GCGGCAGTTC CAGGCTGATC TGCAGACCGC GGTGGTCATC
    GCTAATGACA TCAAGTCTGA AGCCCAAGAG GAGATTGGTG ACCTGAAGCG CCGCTTACAT
    GAAGCTCAAG AGAAAAACGA GAAGCTCACG AAAGAATTGG AGGAAATAAA GTCACGCAAG
    CAGGAGGAGG AGCGTGGCCG GGTGTATAAC TACATGAATG CTGTGGAGAG AGACCTGGCG
    GCCCTGAGGC AGGGCATGGG GCTGAGCAGA AGGTCCTCCA CGTCCTCGGA GCCGACGCCC
    ACCGTGAAGA CCCTCATCAA GTCCTTCGAC AGCGCATCTC AAGTGCCCAG CCCTGCCGCG
    GCAGCGATGC CCCGGACGCC TCTGAGCCCA AGCCCTATGA AGACGCCCCC TGCGGCCGCC
    GTCTCCCCCA TGCAGAGACA TTCCATCAGT GGACCCATCT CCACGTCCAA ACCCCTGACA
    GCCCTCTCAG ATAAGAGACC AAACTACGGG GAGATCCCTG TGCAAGAGCA TCTGCTGAGA
    ACGTCTTCGA CCAGCCGACC TGCTTCTCTG CCGAGAGTGC CTGCCATGGA GAGCGCAAAG
    ACCCTCTCAG TGTCCAGACG AAGCAGCGAG GAGATGAAGC GCGACGTGTC TGCTCCCGAG
    GGCACGTCGC CCGCCGCGCT CATGGCCATG GGGGCCACCT CGCCGCAGCT GTCGCTCTCC
    TCCTCGCCCA CGGCCTCCGT GACCCCCACC GCCCGGAGCC GCATCAGGGA AGAAAGGAAG
    GATCCTCTGT CGGCCTTGGC GAGAGAATAT GGGGGTTCCA AGAGGAACGC CTTGCTGAAG
    TGGTGTCAGA AGAAAACCGA GGGCTACCAG AACATTGACA TCACCAACTT CAGCAGCAGC
    TGGAACGATG GGCTGGCCTT CTGCGCCCTC CTGCACACCT ACCTCCCCGC ACACATCCCC
    TACCAGGAGC TCAACAGCCA GGACAAGCGG AGGAACTTCA CGCTGGCCTT CCAGGCGGCC
    GAAAGCGTTG GCATCAAGTC CACACTGGAC ATCAACGAGA TGGTGCGGAC CGAGCGGCCC
    GACTGGCAGA ACGTGATGCT CTACGTGACC GCCATCTACA AATACTTCGA GACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93285
    Clone ID Related Accession (Same CDS sequence) XM_015475617.2 , XM_015475617.1
    Accession Version XM_015475617.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104461.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTGGAG GGGTGACCGT GACCAATGGT GTCAAAGGCA AGAAGAACGC CTGCTCCTCG 
    GCCGCGCCAG CCGCGCCCGC GCCTGCCGTG AGCGCCACGG AGAACAGGCC CAGGACCGGC
    ACAGGCACGA GTTCTTCAAC CAAGCGGAGC ACTTGCACAG GTAATAAAGA GCCCAGTTCT
    ACTAGAGAAA GATTGCGTGA GCGCACCCGA CTAAACCAGA GCAAAAAACT ACCTTCTGCA
    GGTCAGGGGG CTAATGACGT GGCGTCGGCC AAACGCTCCC GCAGTCGCAC GGCTGCGGAA
    TGCGACGTCC GCATGAGCAA GTCCAAGTCG GACAATCAGA TCAGTGACAA AGCTGCTCTG
    GAGGCCAGAG TGAAGGATCT TCTCACCTTG GCCAAAACCA AGGACGTGGA GATTTTACAT
    CTGCGGAGCG AGCTCCGGGA CATGCGTGCT CAGCTGGGTA TCAGCGAGGA CCCCCTCACC
    GAGGGCGCCG AGACGTCCGA GGAGAAGGAA GCCAGCACCG TGCACCAGCC AACCGACGTG
    GAGTCCACCT TGCTGCAGCT GCAGGAGCAG AACGCCGCCA TCCGCGAAGA GCTCAACCAG
    CTGAAGAACG AGAACCGGAT GCTGAAGGAC CGGCTGAACG CGCTGGGCTT CTCCCTGGAG
    CAGAGGCTGG ACCATTCCGA AAAGCTGTTT GGCTATCAGT CCCTGAGCCC AGAGATCACC
    CCTGGTAACC AGAGCGACGG CGGCGGGACG CTGACCTCCT CGGTGGAGGG CTCGGCCCCT
    GGCTCAGTGG AGGATCTGCT GAGCCAGGAT GAAAACACGC TCATGGACCA TCAGCACAGC
    AACTCCCTGG ACAACCTGGA CAGTGAGTGC AGTGAGGTGT ACCAGCCCCT CACGTCCAGC
    GACGACGCCC TGGACGCGCC CTCGTCCTCG GAGTCAGAAG GCCTTCCGAG CGTAGAGCGC
    TCTCGCAAGG GCAGCAGCGG GAATGCCAGC GAAGTGTCTG TCGCGTGCCT GACGGAGCGG
    ATACACCAGA TGGAGGAGAA CCAGCACAGC ACGAGCGAGG AGCTGCAGGC CACTCTGCAG
    GAGCTGGCTG ACCTGCAGCA GATCACCCAG GAGCTGAACA GCGAGAATGA GCGGCTCGGC
    GAGGAGAAGG TGATTCTCAT GGAGTCCCTG TGTCAGCAGA GCGACAAGCT GGAGCACTTC
    AGCCGGCAGA TTGAGTATTT CCGCTCCCTC CTGGACGAGC ATCACATTTC CTATGTCATC
    GACGAAGACG TGAAAAGCGG GCGCTACATG GAGCTGGAGC AGCGCTACAT GGACCTCGCT
    GAGAACAGCC GCTTTGAACG GGAGCAGCTC CTTGGCGTCC AGCAGCACCT AAGCAACACC
    CTGAAGATGG CCGAGCAGGA CAACAAGGAG GCCCAGGAGC TGATCGGCGC GCTCAAGGAG
    CGCAGCCACC ACATGGAGCG GGCCGTCGAG TCCGAGCAGA AGGGCAAGGC GGCCTTGGCC
    GCCACCCTGG AGGAGCTCAG AGCCACCCTG GCCAGTGACC AGATCGAGAT GAATCGCCTG
    AAGGCCCAGC TGGAGAACGA GAAGCAGAAA GTGGCGGAGC TGTACTCTAT CCATAACTCT
    GGAGACAAGT CTGACATTCA GGACCTCCTG GAGAGCGTCA GGCTGGACAA GGAGAAAGCA
    GAGACTCTGG CCAGTAGCCT GCAGGAAGAC CTGGCCCACA CCCGGAATGA TGCCAACCGG
    CTGCAGGACG CCATCGCAAA GGTGGAAGAC GAGTACCGAG CCTTCCAAGG GGAAGCTAAG
    AAGCAGATAG AGGACCTGAA CGTGGCCGTA GAGAAGCTAC GCTCAGAGCT GGAAGAAAGA
    GACACAGAGA GGAGTGACAT GAAGGAAACG ATCTTTGAGC TTGAGGATGA AGTGGAGCAG
    CACCGGGCAG TGAAGCTGCA CGACAACCTC ATCATCTCGG ACTTGGAGAA TACGGTTAAA
    AAACTCCAGG ACCAAAAACA TGACATGGAA AGAGAAATCA AGACCCTGCA CAGGCGACTG
    CGGGAGGAAT CAGCAGAATG GCGGCAGTTC CAGGCTGATC TGCAGACCGC GGTGGTCATC
    GCTAATGACA TCAAGTCTGA AGCCCAAGAG GAGATTGGTG ACCTGAAGCG CCGCTTACAT
    GAAGCTCAAG AGAAAAACGA GAAGCTCACG AAAGAATTGG AGGAAATAAA GTCACGCAAG
    CAGGAGGAGG AGCGTGGCCG GGTGTATAAC TACATGAATG CTGTGGAGAG AGACCTGGCG
    GCCCTGAGGC AGGGCATGGG GCTGAGCAGA AGGTCCTCCA CGTCCTCGGA GCCGACGCCC
    ACCGTGAAGA CCCTCATCAA GTCCTTCGAC AGCGCATCTC AAGTGCCCAG CCCTGCCGCG
    GCAGCGATGC CCCGGACGCC TCTGAGCCCA AGCCCTATGA AGACGCCCCC TGCGGCCGCC
    GTCTCCCCCA TGCAGAGACA TTCCATCAGT GGACCCATCT CCACGTCCAA ACCCCTGACA
    GCCCTCTCAG ATAAGAGACC AAACTACGGG GAGATCCCTG TGCAAGAGCA TCTGCTGAGA
    ACGTCTTCGA CCAGCCGACC TGCTTCTCTG CCGAGAGTGC CTGCCATGGA GAGCGCAAAG
    ACCCTCTCAG TGTCCAGACG AAGCAGCGAG GAGATGAAGC GCGACGTGTC TGCTCCCGAG
    GGCACGTCGC CCGCCGCGCT CATGGCCATG GGGGCCACCT CGCCGCAGCT GTCGCTCTCC
    TCCTCGCCCA CGGCCTCCGT GACCCCCACC GCCCGGAGCC GCATCAGGGA AGAAAGGAAG
    GATCCTCTGT CGGCCTTGGC GAGAGAATAT GGGGGTTCCA AGAGGAACGC CTTGCTGAAG
    TGGTGTCAGA AGAAAACCGA GGGCTACCAG AACATTGACA TCACCAACTT CAGCAGCAGC
    TGGAACGATG GGCTGGCCTT CTGCGCCCTC CTGCACACCT ACCTCCCCGC ACACATCCCC
    TACCAGGAGC TCAACAGCCA GGACAAGCGG AGGAACTTCA CGCTGGCCTT CCAGGCGGCC
    GAAAGCGTTG GCATCAAGTC CACACTGGAC ATCAACGAGA TGGTGCGGAC CGAGCGGCCC
    GACTGGCAGA ACGTGATGCT CTACGTGACC GCCATCTACA AATACTTCGA GACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331103.1
    CDS415..3591
    Translation

    Target ORF information:

    RefSeq Version XM_015475617.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1-like (SPECC1L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475617.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTGGAG GGGTGACCGT GACCAATGGT GTCAAAGGCA AGAAGAACGC CTGCTCCTCG 
    GCCGCGCCAG CCGCGCCCGC GCCTGCCGTG AGCGCCACGG AGAACAGGCC CAGGACCGGC
    ACAGGCACGA GTTCTTCAAC CAAGCGGAGC ACTTGCACAG GTAATAAAGA GCCCAGTTCT
    ACTAGAGAAA GATTGCGTGA GCGCACCCGA CTAAACCAGA GCAAAAAACT ACCTTCTGCA
    GGTCAGGGGG CTAATGACGT GGCGTCGGCC AAACGCTCCC GCAGTCGCAC GGCTGCGGAA
    TGCGACGTCC GCATGAGCAA GTCCAAGTCG GACAATCAGA TCAGTGACAA AGCTGCTCTG
    GAGGCCAGAG TGAAGGATCT TCTCACCTTG GCCAAAACCA AGGACGTGGA GATTTTACAT
    CTGCGGAGCG AGCTCCGGGA CATGCGTGCT CAGCTGGGTA TCAGCGAGGA CCCCCTCACC
    GAGGGCGCCG AGACGTCCGA GGAGAAGGAA GCCAGCACCG TGCACCAGCC AACCGACGTG
    GAGTCCACCT TGCTGCAGCT GCAGGAGCAG AACGCCGCCA TCCGCGAAGA GCTCAACCAG
    CTGAAGAACG AGAACCGGAT GCTGAAGGAC CGGCTGAACG CGCTGGGCTT CTCCCTGGAG
    CAGAGGCTGG ACCATTCCGA AAAGCTGTTT GGCTATCAGT CCCTGAGCCC AGAGATCACC
    CCTGGTAACC AGAGCGACGG CGGCGGGACG CTGACCTCCT CGGTGGAGGG CTCGGCCCCT
    GGCTCAGTGG AGGATCTGCT GAGCCAGGAT GAAAACACGC TCATGGACCA TCAGCACAGC
    AACTCCCTGG ACAACCTGGA CAGTGAGTGC AGTGAGGTGT ACCAGCCCCT CACGTCCAGC
    GACGACGCCC TGGACGCGCC CTCGTCCTCG GAGTCAGAAG GCCTTCCGAG CGTAGAGCGC
    TCTCGCAAGG GCAGCAGCGG GAATGCCAGC GAAGTGTCTG TCGCGTGCCT GACGGAGCGG
    ATACACCAGA TGGAGGAGAA CCAGCACAGC ACGAGCGAGG AGCTGCAGGC CACTCTGCAG
    GAGCTGGCTG ACCTGCAGCA GATCACCCAG GAGCTGAACA GCGAGAATGA GCGGCTCGGC
    GAGGAGAAGG TGATTCTCAT GGAGTCCCTG TGTCAGCAGA GCGACAAGCT GGAGCACTTC
    AGCCGGCAGA TTGAGTATTT CCGCTCCCTC CTGGACGAGC ATCACATTTC CTATGTCATC
    GACGAAGACG TGAAAAGCGG GCGCTACATG GAGCTGGAGC AGCGCTACAT GGACCTCGCT
    GAGAACAGCC GCTTTGAACG GGAGCAGCTC CTTGGCGTCC AGCAGCACCT AAGCAACACC
    CTGAAGATGG CCGAGCAGGA CAACAAGGAG GCCCAGGAGC TGATCGGCGC GCTCAAGGAG
    CGCAGCCACC ACATGGAGCG GGCCGTCGAG TCCGAGCAGA AGGGCAAGGC GGCCTTGGCC
    GCCACCCTGG AGGAGCTCAG AGCCACCCTG GCCAGTGACC AGATCGAGAT GAATCGCCTG
    AAGGCCCAGC TGGAGAACGA GAAGCAGAAA GTGGCGGAGC TGTACTCTAT CCATAACTCT
    GGAGACAAGT CTGACATTCA GGACCTCCTG GAGAGCGTCA GGCTGGACAA GGAGAAAGCA
    GAGACTCTGG CCAGTAGCCT GCAGGAAGAC CTGGCCCACA CCCGGAATGA TGCCAACCGG
    CTGCAGGACG CCATCGCAAA GGTGGAAGAC GAGTACCGAG CCTTCCAAGG GGAAGCTAAG
    AAGCAGATAG AGGACCTGAA CGTGGCCGTA GAGAAGCTAC GCTCAGAGCT GGAAGAAAGA
    GACACAGAGA GGAGTGACAT GAAGGAAACG ATCTTTGAGC TTGAGGATGA AGTGGAGCAG
    CACCGGGCAG TGAAGCTGCA CGACAACCTC ATCATCTCGG ACTTGGAGAA TACGGTTAAA
    AAACTCCAGG ACCAAAAACA TGACATGGAA AGAGAAATCA AGACCCTGCA CAGGCGACTG
    CGGGAGGAAT CAGCAGAATG GCGGCAGTTC CAGGCTGATC TGCAGACCGC GGTGGTCATC
    GCTAATGACA TCAAGTCTGA AGCCCAAGAG GAGATTGGTG ACCTGAAGCG CCGCTTACAT
    GAAGCTCAAG AGAAAAACGA GAAGCTCACG AAAGAATTGG AGGAAATAAA GTCACGCAAG
    CAGGAGGAGG AGCGTGGCCG GGTGTATAAC TACATGAATG CTGTGGAGAG AGACCTGGCG
    GCCCTGAGGC AGGGCATGGG GCTGAGCAGA AGGTCCTCCA CGTCCTCGGA GCCGACGCCC
    ACCGTGAAGA CCCTCATCAA GTCCTTCGAC AGCGCATCTC AAGTGCCCAG CCCTGCCGCG
    GCAGCGATGC CCCGGACGCC TCTGAGCCCA AGCCCTATGA AGACGCCCCC TGCGGCCGCC
    GTCTCCCCCA TGCAGAGACA TTCCATCAGT GGACCCATCT CCACGTCCAA ACCCCTGACA
    GCCCTCTCAG ATAAGAGACC AAACTACGGG GAGATCCCTG TGCAAGAGCA TCTGCTGAGA
    ACGTCTTCGA CCAGCCGACC TGCTTCTCTG CCGAGAGTGC CTGCCATGGA GAGCGCAAAG
    ACCCTCTCAG TGTCCAGACG AAGCAGCGAG GAGATGAAGC GCGACGTGTC TGCTCCCGAG
    GGCACGTCGC CCGCCGCGCT CATGGCCATG GGGGCCACCT CGCCGCAGCT GTCGCTCTCC
    TCCTCGCCCA CGGCCTCCGT GACCCCCACC GCCCGGAGCC GCATCAGGGA AGAAAGGAAG
    GATCCTCTGT CGGCCTTGGC GAGAGAATAT GGGGGTTCCA AGAGGAACGC CTTGCTGAAG
    TGGTGTCAGA AGAAAACCGA GGGCTACCAG AACATTGACA TCACCAACTT CAGCAGCAGC
    TGGAACGATG GGCTGGCCTT CTGCGCCCTC CTGCACACCT ACCTCCCCGC ACACATCCCC
    TACCAGGAGC TCAACAGCCA GGACAAGCGG AGGAACTTCA CGCTGGCCTT CCAGGCGGCC
    GAAAGCGTTG GCATCAAGTC CACACTGGAC ATCAACGAGA TGGTGCGGAC CGAGCGGCCC
    GACTGGCAGA ACGTGATGCT CTACGTGACC GCCATCTACA AATACTTCGA GACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93286
    Clone ID Related Accession (Same CDS sequence) XM_015475618.1 , XM_015475618.2
    Accession Version XM_015475618.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3123bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104461.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGA GCATCTGCTG
    AGAACGTCTT CGACCAGCCG ACCTGCTTCT CTGCCGAGAG TGCCTGCCAT GGAGAGCGCA
    AAGACCCTCT CAGTGTCCAG ACGAAGCAGC GAGGAGATGA AGCGCGACGT GTCTGCTCCC
    GAGGGCACGT CGCCCGCCGC GCTCATGGCC ATGGGGGCCA CCTCGCCGCA GCTGTCGCTC
    TCCTCCTCGC CCACGGCCTC CGTGACCCCC ACCGCCCGGA GCCGCATCAG GGAAGAAAGG
    AAGGATCCTC TGTCGGCCTT GGCGAGAGAA TATGGGGGTT CCAAGAGGAA CGCCTTGCTG
    AAGTGGTGTC AGAAGAAAAC CGAGGGCTAC CAGTTGCAGG TGGACAGCAG AGTGATCCAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331104.1
    CDS165..3287
    Translation

    Target ORF information:

    RefSeq Version XM_015475618.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1-like (SPECC1L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475618.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGA GCATCTGCTG
    AGAACGTCTT CGACCAGCCG ACCTGCTTCT CTGCCGAGAG TGCCTGCCAT GGAGAGCGCA
    AAGACCCTCT CAGTGTCCAG ACGAAGCAGC GAGGAGATGA AGCGCGACGT GTCTGCTCCC
    GAGGGCACGT CGCCCGCCGC GCTCATGGCC ATGGGGGCCA CCTCGCCGCA GCTGTCGCTC
    TCCTCCTCGC CCACGGCCTC CGTGACCCCC ACCGCCCGGA GCCGCATCAG GGAAGAAAGG
    AAGGATCCTC TGTCGGCCTT GGCGAGAGAA TATGGGGGTT CCAAGAGGAA CGCCTTGCTG
    AAGTGGTGTC AGAAGAAAAC CGAGGGCTAC CAGTTGCAGG TGGACAGCAG AGTGATCCAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93286
    Clone ID Related Accession (Same CDS sequence) XM_015475618.1 , XM_015475618.2
    Accession Version XM_015475618.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3123bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475618.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGA GCATCTGCTG
    AGAACGTCTT CGACCAGCCG ACCTGCTTCT CTGCCGAGAG TGCCTGCCAT GGAGAGCGCA
    AAGACCCTCT CAGTGTCCAG ACGAAGCAGC GAGGAGATGA AGCGCGACGT GTCTGCTCCC
    GAGGGCACGT CGCCCGCCGC GCTCATGGCC ATGGGGGCCA CCTCGCCGCA GCTGTCGCTC
    TCCTCCTCGC CCACGGCCTC CGTGACCCCC ACCGCCCGGA GCCGCATCAG GGAAGAAAGG
    AAGGATCCTC TGTCGGCCTT GGCGAGAGAA TATGGGGGTT CCAAGAGGAA CGCCTTGCTG
    AAGTGGTGTC AGAAGAAAAC CGAGGGCTAC CAGTTGCAGG TGGACAGCAG AGTGATCCAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331104.1
    CDS184..3306
    Translation

    Target ORF information:

    RefSeq Version XM_015475618.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475618.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGA GCATCTGCTG
    AGAACGTCTT CGACCAGCCG ACCTGCTTCT CTGCCGAGAG TGCCTGCCAT GGAGAGCGCA
    AAGACCCTCT CAGTGTCCAG ACGAAGCAGC GAGGAGATGA AGCGCGACGT GTCTGCTCCC
    GAGGGCACGT CGCCCGCCGC GCTCATGGCC ATGGGGGCCA CCTCGCCGCA GCTGTCGCTC
    TCCTCCTCGC CCACGGCCTC CGTGACCCCC ACCGCCCGGA GCCGCATCAG GGAAGAAAGG
    AAGGATCCTC TGTCGGCCTT GGCGAGAGAA TATGGGGGTT CCAAGAGGAA CGCCTTGCTG
    AAGTGGTGTC AGAAGAAAAC CGAGGGCTAC CAGTTGCAGG TGGACAGCAG AGTGATCCAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28162
    Clone ID Related Accession (Same CDS sequence) XM_005218227.3 , NM_001205864.1 , XM_005218226.4 , XM_005218227.2 , XM_005218226.3
    Accession Version NM_001205864.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product cytospin-A
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000017.1. On Apr 20, 2011 this sequence version replaced XM_597976.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR5571263.24935.1, SRR6764281.40482.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGA GCATCTGCTG
    AGAACGTCTT CGACCAGCCG ACCTGCTTCT CTGCCGAGAG TGCCTGCCAT GGAGAGCGCA
    AAGACCCTCT CAGTGTCCAG ACGAAGCAGC GAGGAGATGA AGCGCGACGT GTCTGCTCCC
    GAGGGCACGT CGCCCGCCGC GCTCATGGCC ATGGGGGCCA CCTCGCCGCA GCTGTCGCTC
    TCCTCCTCGC CCACGGCCTC CGTGACCCCC ACCGCCCGGA GCCGCATCAG GGAAGAAAGG
    AAGGATCCTC TGTCGGCCTT GGCGAGAGAA TATGGGGGTT CCAAGAGGAA CGCCTTGCTG
    AAGTGGTGTC AGAAGAAAAC CGAGGGCTAC CAGAACATTG ACATCACCAA CTTCAGCAGC
    AGCTGGAACG ATGGGCTGGC CTTCTGCGCC CTCCTGCACA CCTACCTCCC CGCACACATC
    CCCTACCAGG AGCTCAACAG CCAGGACAAG CGGAGGAACT TCACGCTGGC CTTCCAGGCG
    GCCGAAAGCG TTGGCATCAA GTCCACACTG GACATCAACG AGATGGTGCG GACCGAGCGG
    CCCGACTGGC AGAACGTGAT GCTCTACGTG ACCGCCATCT ACAAATACTT CGAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192793.1
    CDS1..3360
    Translation

    Target ORF information:

    RefSeq Version NM_001205864.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205864.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGA GCATCTGCTG
    AGAACGTCTT CGACCAGCCG ACCTGCTTCT CTGCCGAGAG TGCCTGCCAT GGAGAGCGCA
    AAGACCCTCT CAGTGTCCAG ACGAAGCAGC GAGGAGATGA AGCGCGACGT GTCTGCTCCC
    GAGGGCACGT CGCCCGCCGC GCTCATGGCC ATGGGGGCCA CCTCGCCGCA GCTGTCGCTC
    TCCTCCTCGC CCACGGCCTC CGTGACCCCC ACCGCCCGGA GCCGCATCAG GGAAGAAAGG
    AAGGATCCTC TGTCGGCCTT GGCGAGAGAA TATGGGGGTT CCAAGAGGAA CGCCTTGCTG
    AAGTGGTGTC AGAAGAAAAC CGAGGGCTAC CAGAACATTG ACATCACCAA CTTCAGCAGC
    AGCTGGAACG ATGGGCTGGC CTTCTGCGCC CTCCTGCACA CCTACCTCCC CGCACACATC
    CCCTACCAGG AGCTCAACAG CCAGGACAAG CGGAGGAACT TCACGCTGGC CTTCCAGGCG
    GCCGAAAGCG TTGGCATCAA GTCCACACTG GACATCAACG AGATGGTGCG GACCGAGCGG
    CCCGACTGGC AGAACGTGAT GCTCTACGTG ACCGCCATCT ACAAATACTT CGAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28162
    Clone ID Related Accession (Same CDS sequence) XM_005218227.3 , NM_001205864.1 , XM_005218226.4 , XM_005218227.2 , XM_005218226.3
    Accession Version XM_005218227.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218227.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGA GCATCTGCTG
    AGAACGTCTT CGACCAGCCG ACCTGCTTCT CTGCCGAGAG TGCCTGCCAT GGAGAGCGCA
    AAGACCCTCT CAGTGTCCAG ACGAAGCAGC GAGGAGATGA AGCGCGACGT GTCTGCTCCC
    GAGGGCACGT CGCCCGCCGC GCTCATGGCC ATGGGGGCCA CCTCGCCGCA GCTGTCGCTC
    TCCTCCTCGC CCACGGCCTC CGTGACCCCC ACCGCCCGGA GCCGCATCAG GGAAGAAAGG
    AAGGATCCTC TGTCGGCCTT GGCGAGAGAA TATGGGGGTT CCAAGAGGAA CGCCTTGCTG
    AAGTGGTGTC AGAAGAAAAC CGAGGGCTAC CAGAACATTG ACATCACCAA CTTCAGCAGC
    AGCTGGAACG ATGGGCTGGC CTTCTGCGCC CTCCTGCACA CCTACCTCCC CGCACACATC
    CCCTACCAGG AGCTCAACAG CCAGGACAAG CGGAGGAACT TCACGCTGGC CTTCCAGGCG
    GCCGAAAGCG TTGGCATCAA GTCCACACTG GACATCAACG AGATGGTGCG GACCGAGCGG
    CCCGACTGGC AGAACGTGAT GCTCTACGTG ACCGCCATCT ACAAATACTT CGAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218284.1
    CDS648..4007
    Translation

    Target ORF information:

    RefSeq Version XM_005218227.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218227.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGA GCATCTGCTG
    AGAACGTCTT CGACCAGCCG ACCTGCTTCT CTGCCGAGAG TGCCTGCCAT GGAGAGCGCA
    AAGACCCTCT CAGTGTCCAG ACGAAGCAGC GAGGAGATGA AGCGCGACGT GTCTGCTCCC
    GAGGGCACGT CGCCCGCCGC GCTCATGGCC ATGGGGGCCA CCTCGCCGCA GCTGTCGCTC
    TCCTCCTCGC CCACGGCCTC CGTGACCCCC ACCGCCCGGA GCCGCATCAG GGAAGAAAGG
    AAGGATCCTC TGTCGGCCTT GGCGAGAGAA TATGGGGGTT CCAAGAGGAA CGCCTTGCTG
    AAGTGGTGTC AGAAGAAAAC CGAGGGCTAC CAGAACATTG ACATCACCAA CTTCAGCAGC
    AGCTGGAACG ATGGGCTGGC CTTCTGCGCC CTCCTGCACA CCTACCTCCC CGCACACATC
    CCCTACCAGG AGCTCAACAG CCAGGACAAG CGGAGGAACT TCACGCTGGC CTTCCAGGCG
    GCCGAAAGCG TTGGCATCAA GTCCACACTG GACATCAACG AGATGGTGCG GACCGAGCGG
    CCCGACTGGC AGAACGTGAT GCTCTACGTG ACCGCCATCT ACAAATACTT CGAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28162
    Clone ID Related Accession (Same CDS sequence) XM_005218227.3 , NM_001205864.1 , XM_005218226.4 , XM_005218227.2 , XM_005218226.3
    Accession Version XM_005218226.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218226.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGA GCATCTGCTG
    AGAACGTCTT CGACCAGCCG ACCTGCTTCT CTGCCGAGAG TGCCTGCCAT GGAGAGCGCA
    AAGACCCTCT CAGTGTCCAG ACGAAGCAGC GAGGAGATGA AGCGCGACGT GTCTGCTCCC
    GAGGGCACGT CGCCCGCCGC GCTCATGGCC ATGGGGGCCA CCTCGCCGCA GCTGTCGCTC
    TCCTCCTCGC CCACGGCCTC CGTGACCCCC ACCGCCCGGA GCCGCATCAG GGAAGAAAGG
    AAGGATCCTC TGTCGGCCTT GGCGAGAGAA TATGGGGGTT CCAAGAGGAA CGCCTTGCTG
    AAGTGGTGTC AGAAGAAAAC CGAGGGCTAC CAGAACATTG ACATCACCAA CTTCAGCAGC
    AGCTGGAACG ATGGGCTGGC CTTCTGCGCC CTCCTGCACA CCTACCTCCC CGCACACATC
    CCCTACCAGG AGCTCAACAG CCAGGACAAG CGGAGGAACT TCACGCTGGC CTTCCAGGCG
    GCCGAAAGCG TTGGCATCAA GTCCACACTG GACATCAACG AGATGGTGCG GACCGAGCGG
    CCCGACTGGC AGAACGTGAT GCTCTACGTG ACCGCCATCT ACAAATACTT CGAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218283.1
    CDS183..3542
    Translation

    Target ORF information:

    RefSeq Version XM_005218226.4
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218226.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGA GCATCTGCTG
    AGAACGTCTT CGACCAGCCG ACCTGCTTCT CTGCCGAGAG TGCCTGCCAT GGAGAGCGCA
    AAGACCCTCT CAGTGTCCAG ACGAAGCAGC GAGGAGATGA AGCGCGACGT GTCTGCTCCC
    GAGGGCACGT CGCCCGCCGC GCTCATGGCC ATGGGGGCCA CCTCGCCGCA GCTGTCGCTC
    TCCTCCTCGC CCACGGCCTC CGTGACCCCC ACCGCCCGGA GCCGCATCAG GGAAGAAAGG
    AAGGATCCTC TGTCGGCCTT GGCGAGAGAA TATGGGGGTT CCAAGAGGAA CGCCTTGCTG
    AAGTGGTGTC AGAAGAAAAC CGAGGGCTAC CAGAACATTG ACATCACCAA CTTCAGCAGC
    AGCTGGAACG ATGGGCTGGC CTTCTGCGCC CTCCTGCACA CCTACCTCCC CGCACACATC
    CCCTACCAGG AGCTCAACAG CCAGGACAAG CGGAGGAACT TCACGCTGGC CTTCCAGGCG
    GCCGAAAGCG TTGGCATCAA GTCCACACTG GACATCAACG AGATGGTGCG GACCGAGCGG
    CCCGACTGGC AGAACGTGAT GCTCTACGTG ACCGCCATCT ACAAATACTT CGAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28162
    Clone ID Related Accession (Same CDS sequence) XM_005218227.3 , NM_001205864.1 , XM_005218226.4 , XM_005218227.2 , XM_005218226.3
    Accession Version XM_005218227.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104461.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005218227.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGA GCATCTGCTG
    AGAACGTCTT CGACCAGCCG ACCTGCTTCT CTGCCGAGAG TGCCTGCCAT GGAGAGCGCA
    AAGACCCTCT CAGTGTCCAG ACGAAGCAGC GAGGAGATGA AGCGCGACGT GTCTGCTCCC
    GAGGGCACGT CGCCCGCCGC GCTCATGGCC ATGGGGGCCA CCTCGCCGCA GCTGTCGCTC
    TCCTCCTCGC CCACGGCCTC CGTGACCCCC ACCGCCCGGA GCCGCATCAG GGAAGAAAGG
    AAGGATCCTC TGTCGGCCTT GGCGAGAGAA TATGGGGGTT CCAAGAGGAA CGCCTTGCTG
    AAGTGGTGTC AGAAGAAAAC CGAGGGCTAC CAGAACATTG ACATCACCAA CTTCAGCAGC
    AGCTGGAACG ATGGGCTGGC CTTCTGCGCC CTCCTGCACA CCTACCTCCC CGCACACATC
    CCCTACCAGG AGCTCAACAG CCAGGACAAG CGGAGGAACT TCACGCTGGC CTTCCAGGCG
    GCCGAAAGCG TTGGCATCAA GTCCACACTG GACATCAACG AGATGGTGCG GACCGAGCGG
    CCCGACTGGC AGAACGTGAT GCTCTACGTG ACCGCCATCT ACAAATACTT CGAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218284.1
    CDS275..3634
    Translation

    Target ORF information:

    RefSeq Version XM_005218227.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1-like (SPECC1L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218227.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGA GCATCTGCTG
    AGAACGTCTT CGACCAGCCG ACCTGCTTCT CTGCCGAGAG TGCCTGCCAT GGAGAGCGCA
    AAGACCCTCT CAGTGTCCAG ACGAAGCAGC GAGGAGATGA AGCGCGACGT GTCTGCTCCC
    GAGGGCACGT CGCCCGCCGC GCTCATGGCC ATGGGGGCCA CCTCGCCGCA GCTGTCGCTC
    TCCTCCTCGC CCACGGCCTC CGTGACCCCC ACCGCCCGGA GCCGCATCAG GGAAGAAAGG
    AAGGATCCTC TGTCGGCCTT GGCGAGAGAA TATGGGGGTT CCAAGAGGAA CGCCTTGCTG
    AAGTGGTGTC AGAAGAAAAC CGAGGGCTAC CAGAACATTG ACATCACCAA CTTCAGCAGC
    AGCTGGAACG ATGGGCTGGC CTTCTGCGCC CTCCTGCACA CCTACCTCCC CGCACACATC
    CCCTACCAGG AGCTCAACAG CCAGGACAAG CGGAGGAACT TCACGCTGGC CTTCCAGGCG
    GCCGAAAGCG TTGGCATCAA GTCCACACTG GACATCAACG AGATGGTGCG GACCGAGCGG
    CCCGACTGGC AGAACGTGAT GCTCTACGTG ACCGCCATCT ACAAATACTT CGAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28162
    Clone ID Related Accession (Same CDS sequence) XM_005218227.3 , NM_001205864.1 , XM_005218226.4 , XM_005218227.2 , XM_005218226.3
    Accession Version XM_005218226.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104461.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218226.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGA GCATCTGCTG
    AGAACGTCTT CGACCAGCCG ACCTGCTTCT CTGCCGAGAG TGCCTGCCAT GGAGAGCGCA
    AAGACCCTCT CAGTGTCCAG ACGAAGCAGC GAGGAGATGA AGCGCGACGT GTCTGCTCCC
    GAGGGCACGT CGCCCGCCGC GCTCATGGCC ATGGGGGCCA CCTCGCCGCA GCTGTCGCTC
    TCCTCCTCGC CCACGGCCTC CGTGACCCCC ACCGCCCGGA GCCGCATCAG GGAAGAAAGG
    AAGGATCCTC TGTCGGCCTT GGCGAGAGAA TATGGGGGTT CCAAGAGGAA CGCCTTGCTG
    AAGTGGTGTC AGAAGAAAAC CGAGGGCTAC CAGAACATTG ACATCACCAA CTTCAGCAGC
    AGCTGGAACG ATGGGCTGGC CTTCTGCGCC CTCCTGCACA CCTACCTCCC CGCACACATC
    CCCTACCAGG AGCTCAACAG CCAGGACAAG CGGAGGAACT TCACGCTGGC CTTCCAGGCG
    GCCGAAAGCG TTGGCATCAA GTCCACACTG GACATCAACG AGATGGTGCG GACCGAGCGG
    CCCGACTGGC AGAACGTGAT GCTCTACGTG ACCGCCATCT ACAAATACTT CGAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218283.1
    CDS163..3522
    Translation

    Target ORF information:

    RefSeq Version XM_005218226.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1-like (SPECC1L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218226.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTAATAA AGAGCCCAGT
    TCTACTAGAG AAAGATTGCG TGAGCGCACC CGACTAAACC AGAGCAAAAA ACTACCTTCT
    GCAGGTCAGG GGGCTAATGA CGTGGCGTCG GCCAAACGCT CCCGCAGTCG CACGGCTGCG
    GAATGCGACG TCCGCATGAG CAAGTCCAAG TCGGACAATC AGATCAGTGA CAAAGCTGCT
    CTGGAGGCCA GAGTGAAGGA TCTTCTCACC TTGGCCAAAA CCAAGGACGT GGAGATTTTA
    CATCTGCGGA GCGAGCTCCG GGACATGCGT GCTCAGCTGG GTATCAGCGA GGACCCCCTC
    ACCGAGGGCG CCGAGACGTC CGAGGAGAAG GAAGCCAGCA CCGTGCACCA GCCAACCGAC
    GTGGAGTCCA CCTTGCTGCA GCTGCAGGAG CAGAACGCCG CCATCCGCGA AGAGCTCAAC
    CAGCTGAAGA ACGAGAACCG GATGCTGAAG GACCGGCTGA ACGCGCTGGG CTTCTCCCTG
    GAGCAGAGGC TGGACCATTC CGAAAAGCTG TTTGGCTATC AGTCCCTGAG CCCAGAGATC
    ACCCCTGGTA ACCAGAGCGA CGGCGGCGGG ACGCTGACCT CCTCGGTGGA GGGCTCGGCC
    CCTGGCTCAG TGGAGGATCT GCTGAGCCAG GATGAAAACA CGCTCATGGA CCATCAGCAC
    AGCAACTCCC TGGACAACCT GGACAGTGAG TGCAGTGAGG TGTACCAGCC CCTCACGTCC
    AGCGACGACG CCCTGGACGC GCCCTCGTCC TCGGAGTCAG AAGGCCTTCC GAGCGTAGAG
    CGCTCTCGCA AGGGCAGCAG CGGGAATGCC AGCGAAGTGT CTGTCGCGTG CCTGACGGAG
    CGGATACACC AGATGGAGGA GAACCAGCAC AGCACGAGCG AGGAGCTGCA GGCCACTCTG
    CAGGAGCTGG CTGACCTGCA GCAGATCACC CAGGAGCTGA ACAGCGAGAA TGAGCGGCTC
    GGCGAGGAGA AGGTGATTCT CATGGAGTCC CTGTGTCAGC AGAGCGACAA GCTGGAGCAC
    TTCAGCCGGC AGATTGAGTA TTTCCGCTCC CTCCTGGACG AGCATCACAT TTCCTATGTC
    ATCGACGAAG ACGTGAAAAG CGGGCGCTAC ATGGAGCTGG AGCAGCGCTA CATGGACCTC
    GCTGAGAACA GCCGCTTTGA ACGGGAGCAG CTCCTTGGCG TCCAGCAGCA CCTAAGCAAC
    ACCCTGAAGA TGGCCGAGCA GGACAACAAG GAGGCCCAGG AGCTGATCGG CGCGCTCAAG
    GAGCGCAGCC ACCACATGGA GCGGGCCGTC GAGTCCGAGC AGAAGGGCAA GGCGGCCTTG
    GCCGCCACCC TGGAGGAGCT CAGAGCCACC CTGGCCAGTG ACCAGATCGA GATGAATCGC
    CTGAAGGCCC AGCTGGAGAA CGAGAAGCAG AAAGTGGCGG AGCTGTACTC TATCCATAAC
    TCTGGAGACA AGTCTGACAT TCAGGACCTC CTGGAGAGCG TCAGGCTGGA CAAGGAGAAA
    GCAGAGACTC TGGCCAGTAG CCTGCAGGAA GACCTGGCCC ACACCCGGAA TGATGCCAAC
    CGGCTGCAGG ACGCCATCGC AAAGGTGGAA GACGAGTACC GAGCCTTCCA AGGGGAAGCT
    AAGAAGCAGA TAGAGGACCT GAACGTGGCC GTAGAGAAGC TACGCTCAGA GCTGGAAGAA
    AGAGACACAG AGAGGAGTGA CATGAAGGAA ACGATCTTTG AGCTTGAGGA TGAAGTGGAG
    CAGCACCGGG CAGTGAAGCT GCACGACAAC CTCATCATCT CGGACTTGGA GAATACGGTT
    AAAAAACTCC AGGACCAAAA ACATGACATG GAAAGAGAAA TCAAGACCCT GCACAGGCGA
    CTGCGGGAGG AATCAGCAGA ATGGCGGCAG TTCCAGGCTG ATCTGCAGAC CGCGGTGGTC
    ATCGCTAATG ACATCAAGTC TGAAGCCCAA GAGGAGATTG GTGACCTGAA GCGCCGCTTA
    CATGAAGCTC AAGAGAAAAA CGAGAAGCTC ACGAAAGAAT TGGAGGAAAT AAAGTCACGC
    AAGCAGGAGG AGGAGCGTGG CCGGGTGTAT AACTACATGA ATGCTGTGGA GAGAGACCTG
    GCGGCCCTGA GGCAGGGCAT GGGGCTGAGC AGAAGGTCCT CCACGTCCTC GGAGCCGACG
    CCCACCGTGA AGACCCTCAT CAAGTCCTTC GACAGCGCAT CTCAAGTGCC CAGCCCTGCC
    GCGGCAGCGA TGCCCCGGAC GCCTCTGAGC CCAAGCCCTA TGAAGACGCC CCCTGCGGCC
    GCCGTCTCCC CCATGCAGAG ACATTCCATC AGTGGACCCA TCTCCACGTC CAAACCCCTG
    ACAGCCCTCT CAGATAAGAG ACCAAACTAC GGGGAGATCC CTGTGCAAGA GCATCTGCTG
    AGAACGTCTT CGACCAGCCG ACCTGCTTCT CTGCCGAGAG TGCCTGCCAT GGAGAGCGCA
    AAGACCCTCT CAGTGTCCAG ACGAAGCAGC GAGGAGATGA AGCGCGACGT GTCTGCTCCC
    GAGGGCACGT CGCCCGCCGC GCTCATGGCC ATGGGGGCCA CCTCGCCGCA GCTGTCGCTC
    TCCTCCTCGC CCACGGCCTC CGTGACCCCC ACCGCCCGGA GCCGCATCAG GGAAGAAAGG
    AAGGATCCTC TGTCGGCCTT GGCGAGAGAA TATGGGGGTT CCAAGAGGAA CGCCTTGCTG
    AAGTGGTGTC AGAAGAAAAC CGAGGGCTAC CAGAACATTG ACATCACCAA CTTCAGCAGC
    AGCTGGAACG ATGGGCTGGC CTTCTGCGCC CTCCTGCACA CCTACCTCCC CGCACACATC
    CCCTACCAGG AGCTCAACAG CCAGGACAAG CGGAGGAACT TCACGCTGGC CTTCCAGGCG
    GCCGAAAGCG TTGGCATCAA GTCCACACTG GACATCAACG AGATGGTGCG GACCGAGCGG
    CCCGACTGGC AGAACGTGAT GCTCTACGTG ACCGCCATCT ACAAATACTT CGAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28163
    Clone ID Related Accession (Same CDS sequence) XM_005218228.3 , XM_005218228.4
    Accession Version XM_005218228.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cytospin-A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104461.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218228.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTCAGGG GGCTAATGAC
    GTGGCGTCGG CCAAACGCTC CCGCAGTCGC ACGGCTGCGG AATGCGACGT CCGCATGAGC
    AAGTCCAAGT CGGACAATCA GATCAGTGAC AAAGCTGCTC TGGAGGCCAG AGTGAAGGAT
    CTTCTCACCT TGGCCAAAAC CAAGGACGTG GAGATTTTAC ATCTGCGGAG CGAGCTCCGG
    GACATGCGTG CTCAGCTGGG TATCAGCGAG GACCCCCTCA CCGAGGGCGC CGAGACGTCC
    GAGGAGAAGG AAGCCAGCAC CGTGCACCAG CCAACCGACG TGGAGTCCAC CTTGCTGCAG
    CTGCAGGAGC AGAACGCCGC CATCCGCGAA GAGCTCAACC AGCTGAAGAA CGAGAACCGG
    ATGCTGAAGG ACCGGCTGAA CGCGCTGGGC TTCTCCCTGG AGCAGAGGCT GGACCATTCC
    GAAAAGCTGT TTGGCTATCA GTCCCTGAGC CCAGAGATCA CCCCTGGTAA CCAGAGCGAC
    GGCGGCGGGA CGCTGACCTC CTCGGTGGAG GGCTCGGCCC CTGGCTCAGT GGAGGATCTG
    CTGAGCCAGG ATGAAAACAC GCTCATGGAC CATCAGCACA GCAACTCCCT GGACAACCTG
    GACAGTGAGT GCAGTGAGGT GTACCAGCCC CTCACGTCCA GCGACGACGC CCTGGACGCG
    CCCTCGTCCT CGGAGTCAGA AGGCCTTCCG AGCGTAGAGC GCTCTCGCAA GGGCAGCAGC
    GGGAATGCCA GCGAAGTGTC TGTCGCGTGC CTGACGGAGC GGATACACCA GATGGAGGAG
    AACCAGCACA GCACGAGCGA GGAGCTGCAG GCCACTCTGC AGGAGCTGGC TGACCTGCAG
    CAGATCACCC AGGAGCTGAA CAGCGAGAAT GAGCGGCTCG GCGAGGAGAA GGTGATTCTC
    ATGGAGTCCC TGTGTCAGCA GAGCGACAAG CTGGAGCACT TCAGCCGGCA GATTGAGTAT
    TTCCGCTCCC TCCTGGACGA GCATCACATT TCCTATGTCA TCGACGAAGA CGTGAAAAGC
    GGGCGCTACA TGGAGCTGGA GCAGCGCTAC ATGGACCTCG CTGAGAACAG CCGCTTTGAA
    CGGGAGCAGC TCCTTGGCGT CCAGCAGCAC CTAAGCAACA CCCTGAAGAT GGCCGAGCAG
    GACAACAAGG AGGCCCAGGA GCTGATCGGC GCGCTCAAGG AGCGCAGCCA CCACATGGAG
    CGGGCCGTCG AGTCCGAGCA GAAGGGCAAG GCGGCCTTGG CCGCCACCCT GGAGGAGCTC
    AGAGCCACCC TGGCCAGTGA CCAGATCGAG ATGAATCGCC TGAAGGCCCA GCTGGAGAAC
    GAGAAGCAGA AAGTGGCGGA GCTGTACTCT ATCCATAACT CTGGAGACAA GTCTGACATT
    CAGGACCTCC TGGAGAGCGT CAGGCTGGAC AAGGAGAAAG CAGAGACTCT GGCCAGTAGC
    CTGCAGGAAG ACCTGGCCCA CACCCGGAAT GATGCCAACC GGCTGCAGGA CGCCATCGCA
    AAGGTGGAAG ACGAGTACCG AGCCTTCCAA GGGGAAGCTA AGAAGCAGAT AGAGGACCTG
    AACGTGGCCG TAGAGAAGCT ACGCTCAGAG CTGGAAGAAA GAGACACAGA GAGGAGTGAC
    ATGAAGGAAA CGATCTTTGA GCTTGAGGAT GAAGTGGAGC AGCACCGGGC AGTGAAGCTG
    CACGACAACC TCATCATCTC GGACTTGGAG AATACGGTTA AAAAACTCCA GGACCAAAAA
    CATGACATGG AAAGAGAAAT CAAGACCCTG CACAGGCGAC TGCGGGAGGA ATCAGCAGAA
    TGGCGGCAGT TCCAGGCTGA TCTGCAGACC GCGGTGGTCA TCGCTAATGA CATCAAGTCT
    GAAGCCCAAG AGGAGATTGG TGACCTGAAG CGCCGCTTAC ATGAAGCTCA AGAGAAAAAC
    GAGAAGCTCA CGAAAGAATT GGAGGAAATA AAGTCACGCA AGCAGGAGGA GGAGCGTGGC
    CGGGTGTATA ACTACATGAA TGCTGTGGAG AGAGACCTGG CGGCCCTGAG GCAGGGCATG
    GGGCTGAGCA GAAGGTCCTC CACGTCCTCG GAGCCGACGC CCACCGTGAA GACCCTCATC
    AAGTCCTTCG ACAGCGCATC TCAAGTGCCC AGCCCTGCCG CGGCAGCGAT GCCCCGGACG
    CCTCTGAGCC CAAGCCCTAT GAAGACGCCC CCTGCGGCCG CCGTCTCCCC CATGCAGAGA
    CATTCCATCA GTGGACCCAT CTCCACGTCC AAACCCCTGA CAGCCCTCTC AGATAAGAGA
    CCAAACTACG GGGAGATCCC TGTGCAAGAG CATCTGCTGA GAACGTCTTC GACCAGCCGA
    CCTGCTTCTC TGCCGAGAGT GCCTGCCATG GAGAGCGCAA AGACCCTCTC AGTGTCCAGA
    CGAAGCAGCG AGGAGATGAA GCGCGACGTG TCTGCTCCCG AGGGCACGTC GCCCGCCGCG
    CTCATGGCCA TGGGGGCCAC CTCGCCGCAG CTGTCGCTCT CCTCCTCGCC CACGGCCTCC
    GTGACCCCCA CCGCCCGGAG CCGCATCAGG GAAGAAAGGA AGGATCCTCT GTCGGCCTTG
    GCGAGAGAAT ATGGGGGTTC CAAGAGGAAC GCCTTGCTGA AGTGGTGTCA GAAGAAAACC
    GAGGGCTACC AGAACATTGA CATCACCAAC TTCAGCAGCA GCTGGAACGA TGGGCTGGCC
    TTCTGCGCCC TCCTGCACAC CTACCTCCCC GCACACATCC CCTACCAGGA GCTCAACAGC
    CAGGACAAGC GGAGGAACTT CACGCTGGCC TTCCAGGCGG CCGAAAGCGT TGGCATCAAG
    TCCACACTGG ACATCAACGA GATGGTGCGG ACCGAGCGGC CCGACTGGCA GAACGTGATG
    CTCTACGTGA CCGCCATCTA CAAATACTTC GAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218285.1
    CDS163..3441
    Translation

    Target ORF information:

    RefSeq Version XM_005218228.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1-like (SPECC1L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218228.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTCAGGG GGCTAATGAC
    GTGGCGTCGG CCAAACGCTC CCGCAGTCGC ACGGCTGCGG AATGCGACGT CCGCATGAGC
    AAGTCCAAGT CGGACAATCA GATCAGTGAC AAAGCTGCTC TGGAGGCCAG AGTGAAGGAT
    CTTCTCACCT TGGCCAAAAC CAAGGACGTG GAGATTTTAC ATCTGCGGAG CGAGCTCCGG
    GACATGCGTG CTCAGCTGGG TATCAGCGAG GACCCCCTCA CCGAGGGCGC CGAGACGTCC
    GAGGAGAAGG AAGCCAGCAC CGTGCACCAG CCAACCGACG TGGAGTCCAC CTTGCTGCAG
    CTGCAGGAGC AGAACGCCGC CATCCGCGAA GAGCTCAACC AGCTGAAGAA CGAGAACCGG
    ATGCTGAAGG ACCGGCTGAA CGCGCTGGGC TTCTCCCTGG AGCAGAGGCT GGACCATTCC
    GAAAAGCTGT TTGGCTATCA GTCCCTGAGC CCAGAGATCA CCCCTGGTAA CCAGAGCGAC
    GGCGGCGGGA CGCTGACCTC CTCGGTGGAG GGCTCGGCCC CTGGCTCAGT GGAGGATCTG
    CTGAGCCAGG ATGAAAACAC GCTCATGGAC CATCAGCACA GCAACTCCCT GGACAACCTG
    GACAGTGAGT GCAGTGAGGT GTACCAGCCC CTCACGTCCA GCGACGACGC CCTGGACGCG
    CCCTCGTCCT CGGAGTCAGA AGGCCTTCCG AGCGTAGAGC GCTCTCGCAA GGGCAGCAGC
    GGGAATGCCA GCGAAGTGTC TGTCGCGTGC CTGACGGAGC GGATACACCA GATGGAGGAG
    AACCAGCACA GCACGAGCGA GGAGCTGCAG GCCACTCTGC AGGAGCTGGC TGACCTGCAG
    CAGATCACCC AGGAGCTGAA CAGCGAGAAT GAGCGGCTCG GCGAGGAGAA GGTGATTCTC
    ATGGAGTCCC TGTGTCAGCA GAGCGACAAG CTGGAGCACT TCAGCCGGCA GATTGAGTAT
    TTCCGCTCCC TCCTGGACGA GCATCACATT TCCTATGTCA TCGACGAAGA CGTGAAAAGC
    GGGCGCTACA TGGAGCTGGA GCAGCGCTAC ATGGACCTCG CTGAGAACAG CCGCTTTGAA
    CGGGAGCAGC TCCTTGGCGT CCAGCAGCAC CTAAGCAACA CCCTGAAGAT GGCCGAGCAG
    GACAACAAGG AGGCCCAGGA GCTGATCGGC GCGCTCAAGG AGCGCAGCCA CCACATGGAG
    CGGGCCGTCG AGTCCGAGCA GAAGGGCAAG GCGGCCTTGG CCGCCACCCT GGAGGAGCTC
    AGAGCCACCC TGGCCAGTGA CCAGATCGAG ATGAATCGCC TGAAGGCCCA GCTGGAGAAC
    GAGAAGCAGA AAGTGGCGGA GCTGTACTCT ATCCATAACT CTGGAGACAA GTCTGACATT
    CAGGACCTCC TGGAGAGCGT CAGGCTGGAC AAGGAGAAAG CAGAGACTCT GGCCAGTAGC
    CTGCAGGAAG ACCTGGCCCA CACCCGGAAT GATGCCAACC GGCTGCAGGA CGCCATCGCA
    AAGGTGGAAG ACGAGTACCG AGCCTTCCAA GGGGAAGCTA AGAAGCAGAT AGAGGACCTG
    AACGTGGCCG TAGAGAAGCT ACGCTCAGAG CTGGAAGAAA GAGACACAGA GAGGAGTGAC
    ATGAAGGAAA CGATCTTTGA GCTTGAGGAT GAAGTGGAGC AGCACCGGGC AGTGAAGCTG
    CACGACAACC TCATCATCTC GGACTTGGAG AATACGGTTA AAAAACTCCA GGACCAAAAA
    CATGACATGG AAAGAGAAAT CAAGACCCTG CACAGGCGAC TGCGGGAGGA ATCAGCAGAA
    TGGCGGCAGT TCCAGGCTGA TCTGCAGACC GCGGTGGTCA TCGCTAATGA CATCAAGTCT
    GAAGCCCAAG AGGAGATTGG TGACCTGAAG CGCCGCTTAC ATGAAGCTCA AGAGAAAAAC
    GAGAAGCTCA CGAAAGAATT GGAGGAAATA AAGTCACGCA AGCAGGAGGA GGAGCGTGGC
    CGGGTGTATA ACTACATGAA TGCTGTGGAG AGAGACCTGG CGGCCCTGAG GCAGGGCATG
    GGGCTGAGCA GAAGGTCCTC CACGTCCTCG GAGCCGACGC CCACCGTGAA GACCCTCATC
    AAGTCCTTCG ACAGCGCATC TCAAGTGCCC AGCCCTGCCG CGGCAGCGAT GCCCCGGACG
    CCTCTGAGCC CAAGCCCTAT GAAGACGCCC CCTGCGGCCG CCGTCTCCCC CATGCAGAGA
    CATTCCATCA GTGGACCCAT CTCCACGTCC AAACCCCTGA CAGCCCTCTC AGATAAGAGA
    CCAAACTACG GGGAGATCCC TGTGCAAGAG CATCTGCTGA GAACGTCTTC GACCAGCCGA
    CCTGCTTCTC TGCCGAGAGT GCCTGCCATG GAGAGCGCAA AGACCCTCTC AGTGTCCAGA
    CGAAGCAGCG AGGAGATGAA GCGCGACGTG TCTGCTCCCG AGGGCACGTC GCCCGCCGCG
    CTCATGGCCA TGGGGGCCAC CTCGCCGCAG CTGTCGCTCT CCTCCTCGCC CACGGCCTCC
    GTGACCCCCA CCGCCCGGAG CCGCATCAGG GAAGAAAGGA AGGATCCTCT GTCGGCCTTG
    GCGAGAGAAT ATGGGGGTTC CAAGAGGAAC GCCTTGCTGA AGTGGTGTCA GAAGAAAACC
    GAGGGCTACC AGAACATTGA CATCACCAAC TTCAGCAGCA GCTGGAACGA TGGGCTGGCC
    TTCTGCGCCC TCCTGCACAC CTACCTCCCC GCACACATCC CCTACCAGGA GCTCAACAGC
    CAGGACAAGC GGAGGAACTT CACGCTGGCC TTCCAGGCGG CCGAAAGCGT TGGCATCAAG
    TCCACACTGG ACATCAACGA GATGGTGCGG ACCGAGCGGC CCGACTGGCA GAACGTGATG
    CTCTACGTGA CCGCCATCTA CAAATACTTC GAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28163
    Clone ID Related Accession (Same CDS sequence) XM_005218228.3 , XM_005218228.4
    Accession Version XM_005218228.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cytospin-A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218228.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTCAGGG GGCTAATGAC
    GTGGCGTCGG CCAAACGCTC CCGCAGTCGC ACGGCTGCGG AATGCGACGT CCGCATGAGC
    AAGTCCAAGT CGGACAATCA GATCAGTGAC AAAGCTGCTC TGGAGGCCAG AGTGAAGGAT
    CTTCTCACCT TGGCCAAAAC CAAGGACGTG GAGATTTTAC ATCTGCGGAG CGAGCTCCGG
    GACATGCGTG CTCAGCTGGG TATCAGCGAG GACCCCCTCA CCGAGGGCGC CGAGACGTCC
    GAGGAGAAGG AAGCCAGCAC CGTGCACCAG CCAACCGACG TGGAGTCCAC CTTGCTGCAG
    CTGCAGGAGC AGAACGCCGC CATCCGCGAA GAGCTCAACC AGCTGAAGAA CGAGAACCGG
    ATGCTGAAGG ACCGGCTGAA CGCGCTGGGC TTCTCCCTGG AGCAGAGGCT GGACCATTCC
    GAAAAGCTGT TTGGCTATCA GTCCCTGAGC CCAGAGATCA CCCCTGGTAA CCAGAGCGAC
    GGCGGCGGGA CGCTGACCTC CTCGGTGGAG GGCTCGGCCC CTGGCTCAGT GGAGGATCTG
    CTGAGCCAGG ATGAAAACAC GCTCATGGAC CATCAGCACA GCAACTCCCT GGACAACCTG
    GACAGTGAGT GCAGTGAGGT GTACCAGCCC CTCACGTCCA GCGACGACGC CCTGGACGCG
    CCCTCGTCCT CGGAGTCAGA AGGCCTTCCG AGCGTAGAGC GCTCTCGCAA GGGCAGCAGC
    GGGAATGCCA GCGAAGTGTC TGTCGCGTGC CTGACGGAGC GGATACACCA GATGGAGGAG
    AACCAGCACA GCACGAGCGA GGAGCTGCAG GCCACTCTGC AGGAGCTGGC TGACCTGCAG
    CAGATCACCC AGGAGCTGAA CAGCGAGAAT GAGCGGCTCG GCGAGGAGAA GGTGATTCTC
    ATGGAGTCCC TGTGTCAGCA GAGCGACAAG CTGGAGCACT TCAGCCGGCA GATTGAGTAT
    TTCCGCTCCC TCCTGGACGA GCATCACATT TCCTATGTCA TCGACGAAGA CGTGAAAAGC
    GGGCGCTACA TGGAGCTGGA GCAGCGCTAC ATGGACCTCG CTGAGAACAG CCGCTTTGAA
    CGGGAGCAGC TCCTTGGCGT CCAGCAGCAC CTAAGCAACA CCCTGAAGAT GGCCGAGCAG
    GACAACAAGG AGGCCCAGGA GCTGATCGGC GCGCTCAAGG AGCGCAGCCA CCACATGGAG
    CGGGCCGTCG AGTCCGAGCA GAAGGGCAAG GCGGCCTTGG CCGCCACCCT GGAGGAGCTC
    AGAGCCACCC TGGCCAGTGA CCAGATCGAG ATGAATCGCC TGAAGGCCCA GCTGGAGAAC
    GAGAAGCAGA AAGTGGCGGA GCTGTACTCT ATCCATAACT CTGGAGACAA GTCTGACATT
    CAGGACCTCC TGGAGAGCGT CAGGCTGGAC AAGGAGAAAG CAGAGACTCT GGCCAGTAGC
    CTGCAGGAAG ACCTGGCCCA CACCCGGAAT GATGCCAACC GGCTGCAGGA CGCCATCGCA
    AAGGTGGAAG ACGAGTACCG AGCCTTCCAA GGGGAAGCTA AGAAGCAGAT AGAGGACCTG
    AACGTGGCCG TAGAGAAGCT ACGCTCAGAG CTGGAAGAAA GAGACACAGA GAGGAGTGAC
    ATGAAGGAAA CGATCTTTGA GCTTGAGGAT GAAGTGGAGC AGCACCGGGC AGTGAAGCTG
    CACGACAACC TCATCATCTC GGACTTGGAG AATACGGTTA AAAAACTCCA GGACCAAAAA
    CATGACATGG AAAGAGAAAT CAAGACCCTG CACAGGCGAC TGCGGGAGGA ATCAGCAGAA
    TGGCGGCAGT TCCAGGCTGA TCTGCAGACC GCGGTGGTCA TCGCTAATGA CATCAAGTCT
    GAAGCCCAAG AGGAGATTGG TGACCTGAAG CGCCGCTTAC ATGAAGCTCA AGAGAAAAAC
    GAGAAGCTCA CGAAAGAATT GGAGGAAATA AAGTCACGCA AGCAGGAGGA GGAGCGTGGC
    CGGGTGTATA ACTACATGAA TGCTGTGGAG AGAGACCTGG CGGCCCTGAG GCAGGGCATG
    GGGCTGAGCA GAAGGTCCTC CACGTCCTCG GAGCCGACGC CCACCGTGAA GACCCTCATC
    AAGTCCTTCG ACAGCGCATC TCAAGTGCCC AGCCCTGCCG CGGCAGCGAT GCCCCGGACG
    CCTCTGAGCC CAAGCCCTAT GAAGACGCCC CCTGCGGCCG CCGTCTCCCC CATGCAGAGA
    CATTCCATCA GTGGACCCAT CTCCACGTCC AAACCCCTGA CAGCCCTCTC AGATAAGAGA
    CCAAACTACG GGGAGATCCC TGTGCAAGAG CATCTGCTGA GAACGTCTTC GACCAGCCGA
    CCTGCTTCTC TGCCGAGAGT GCCTGCCATG GAGAGCGCAA AGACCCTCTC AGTGTCCAGA
    CGAAGCAGCG AGGAGATGAA GCGCGACGTG TCTGCTCCCG AGGGCACGTC GCCCGCCGCG
    CTCATGGCCA TGGGGGCCAC CTCGCCGCAG CTGTCGCTCT CCTCCTCGCC CACGGCCTCC
    GTGACCCCCA CCGCCCGGAG CCGCATCAGG GAAGAAAGGA AGGATCCTCT GTCGGCCTTG
    GCGAGAGAAT ATGGGGGTTC CAAGAGGAAC GCCTTGCTGA AGTGGTGTCA GAAGAAAACC
    GAGGGCTACC AGAACATTGA CATCACCAAC TTCAGCAGCA GCTGGAACGA TGGGCTGGCC
    TTCTGCGCCC TCCTGCACAC CTACCTCCCC GCACACATCC CCTACCAGGA GCTCAACAGC
    CAGGACAAGC GGAGGAACTT CACGCTGGCC TTCCAGGCGG CCGAAAGCGT TGGCATCAAG
    TCCACACTGG ACATCAACGA GATGGTGCGG ACCGAGCGGC CCGACTGGCA GAACGTGATG
    CTCTACGTGA CCGCCATCTA CAAATACTTC GAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218285.1
    CDS183..3461
    Translation

    Target ORF information:

    RefSeq Version XM_005218228.4
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218228.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAAGAAAG CAAGCAGGAG TGTCGGCTCA GCGCCTAAGG TGTCGGGGAC AAGCAGAGTG 
    CAGGCAGCGG ACAAAGCCAG GCCCGAGAGC AGCTCCTCAG CCTCTGCGGG CAGCAGGCCC
    GTGAAGCCTG GGGCCGCAGC AGCGTTGTCG AAGACCAAGA GCAGTGATGA CCTTTTAGCT
    GGGATGGCTG GAGGGGTGAC CGTGACCAAT GGTGTCAAAG GCAAGAAGAA CGCCTGCTCC
    TCGGCCGCGC CAGCCGCGCC CGCGCCTGCC GTGAGCGCCA CGGAGAACAG GCCCAGGACC
    GGCACAGGCA CGAGTTCTTC AACCAAGCGG AGCACTTGCA CAGGTCAGGG GGCTAATGAC
    GTGGCGTCGG CCAAACGCTC CCGCAGTCGC ACGGCTGCGG AATGCGACGT CCGCATGAGC
    AAGTCCAAGT CGGACAATCA GATCAGTGAC AAAGCTGCTC TGGAGGCCAG AGTGAAGGAT
    CTTCTCACCT TGGCCAAAAC CAAGGACGTG GAGATTTTAC ATCTGCGGAG CGAGCTCCGG
    GACATGCGTG CTCAGCTGGG TATCAGCGAG GACCCCCTCA CCGAGGGCGC CGAGACGTCC
    GAGGAGAAGG AAGCCAGCAC CGTGCACCAG CCAACCGACG TGGAGTCCAC CTTGCTGCAG
    CTGCAGGAGC AGAACGCCGC CATCCGCGAA GAGCTCAACC AGCTGAAGAA CGAGAACCGG
    ATGCTGAAGG ACCGGCTGAA CGCGCTGGGC TTCTCCCTGG AGCAGAGGCT GGACCATTCC
    GAAAAGCTGT TTGGCTATCA GTCCCTGAGC CCAGAGATCA CCCCTGGTAA CCAGAGCGAC
    GGCGGCGGGA CGCTGACCTC CTCGGTGGAG GGCTCGGCCC CTGGCTCAGT GGAGGATCTG
    CTGAGCCAGG ATGAAAACAC GCTCATGGAC CATCAGCACA GCAACTCCCT GGACAACCTG
    GACAGTGAGT GCAGTGAGGT GTACCAGCCC CTCACGTCCA GCGACGACGC CCTGGACGCG
    CCCTCGTCCT CGGAGTCAGA AGGCCTTCCG AGCGTAGAGC GCTCTCGCAA GGGCAGCAGC
    GGGAATGCCA GCGAAGTGTC TGTCGCGTGC CTGACGGAGC GGATACACCA GATGGAGGAG
    AACCAGCACA GCACGAGCGA GGAGCTGCAG GCCACTCTGC AGGAGCTGGC TGACCTGCAG
    CAGATCACCC AGGAGCTGAA CAGCGAGAAT GAGCGGCTCG GCGAGGAGAA GGTGATTCTC
    ATGGAGTCCC TGTGTCAGCA GAGCGACAAG CTGGAGCACT TCAGCCGGCA GATTGAGTAT
    TTCCGCTCCC TCCTGGACGA GCATCACATT TCCTATGTCA TCGACGAAGA CGTGAAAAGC
    GGGCGCTACA TGGAGCTGGA GCAGCGCTAC ATGGACCTCG CTGAGAACAG CCGCTTTGAA
    CGGGAGCAGC TCCTTGGCGT CCAGCAGCAC CTAAGCAACA CCCTGAAGAT GGCCGAGCAG
    GACAACAAGG AGGCCCAGGA GCTGATCGGC GCGCTCAAGG AGCGCAGCCA CCACATGGAG
    CGGGCCGTCG AGTCCGAGCA GAAGGGCAAG GCGGCCTTGG CCGCCACCCT GGAGGAGCTC
    AGAGCCACCC TGGCCAGTGA CCAGATCGAG ATGAATCGCC TGAAGGCCCA GCTGGAGAAC
    GAGAAGCAGA AAGTGGCGGA GCTGTACTCT ATCCATAACT CTGGAGACAA GTCTGACATT
    CAGGACCTCC TGGAGAGCGT CAGGCTGGAC AAGGAGAAAG CAGAGACTCT GGCCAGTAGC
    CTGCAGGAAG ACCTGGCCCA CACCCGGAAT GATGCCAACC GGCTGCAGGA CGCCATCGCA
    AAGGTGGAAG ACGAGTACCG AGCCTTCCAA GGGGAAGCTA AGAAGCAGAT AGAGGACCTG
    AACGTGGCCG TAGAGAAGCT ACGCTCAGAG CTGGAAGAAA GAGACACAGA GAGGAGTGAC
    ATGAAGGAAA CGATCTTTGA GCTTGAGGAT GAAGTGGAGC AGCACCGGGC AGTGAAGCTG
    CACGACAACC TCATCATCTC GGACTTGGAG AATACGGTTA AAAAACTCCA GGACCAAAAA
    CATGACATGG AAAGAGAAAT CAAGACCCTG CACAGGCGAC TGCGGGAGGA ATCAGCAGAA
    TGGCGGCAGT TCCAGGCTGA TCTGCAGACC GCGGTGGTCA TCGCTAATGA CATCAAGTCT
    GAAGCCCAAG AGGAGATTGG TGACCTGAAG CGCCGCTTAC ATGAAGCTCA AGAGAAAAAC
    GAGAAGCTCA CGAAAGAATT GGAGGAAATA AAGTCACGCA AGCAGGAGGA GGAGCGTGGC
    CGGGTGTATA ACTACATGAA TGCTGTGGAG AGAGACCTGG CGGCCCTGAG GCAGGGCATG
    GGGCTGAGCA GAAGGTCCTC CACGTCCTCG GAGCCGACGC CCACCGTGAA GACCCTCATC
    AAGTCCTTCG ACAGCGCATC TCAAGTGCCC AGCCCTGCCG CGGCAGCGAT GCCCCGGACG
    CCTCTGAGCC CAAGCCCTAT GAAGACGCCC CCTGCGGCCG CCGTCTCCCC CATGCAGAGA
    CATTCCATCA GTGGACCCAT CTCCACGTCC AAACCCCTGA CAGCCCTCTC AGATAAGAGA
    CCAAACTACG GGGAGATCCC TGTGCAAGAG CATCTGCTGA GAACGTCTTC GACCAGCCGA
    CCTGCTTCTC TGCCGAGAGT GCCTGCCATG GAGAGCGCAA AGACCCTCTC AGTGTCCAGA
    CGAAGCAGCG AGGAGATGAA GCGCGACGTG TCTGCTCCCG AGGGCACGTC GCCCGCCGCG
    CTCATGGCCA TGGGGGCCAC CTCGCCGCAG CTGTCGCTCT CCTCCTCGCC CACGGCCTCC
    GTGACCCCCA CCGCCCGGAG CCGCATCAGG GAAGAAAGGA AGGATCCTCT GTCGGCCTTG
    GCGAGAGAAT ATGGGGGTTC CAAGAGGAAC GCCTTGCTGA AGTGGTGTCA GAAGAAAACC
    GAGGGCTACC AGAACATTGA CATCACCAAC TTCAGCAGCA GCTGGAACGA TGGGCTGGCC
    TTCTGCGCCC TCCTGCACAC CTACCTCCCC GCACACATCC CCTACCAGGA GCTCAACAGC
    CAGGACAAGC GGAGGAACTT CACGCTGGCC TTCCAGGCGG CCGAAAGCGT TGGCATCAAG
    TCCACACTGG ACATCAACGA GATGGTGCGG ACCGAGCGGC CCGACTGGCA GAACGTGATG
    CTCTACGTGA CCGCCATCTA CAAATACTTC GAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.