FBLN2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FBLN2
Entrez Gene ID	2199
Full Name	fibulin 2
General protein information	Preferred Names fibulin 2 Names fibulin-2 FIBL-2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p25.1
Summary	This gene encodes an extracellular matrix protein, which belongs to the fibulin family. This protein binds various extracellular ligands and calcium. It may play a role during organ development, in particular, during the differentiation of heart, skeletal and neuronal structures. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].
Disorder MIM:	135821

mRNA and Protein(s)

mRNA	Protein	Name
XM_006713026.2	XP_006713089.1	fibulin-2 isoform X1
NM_001998.3	NP_001989.2	fibulin-2 isoform b precursor
NM_001004019.1	NP_001004019.1	fibulin-2 isoform a precursor
NM_001165035.1	NP_001158507.1	fibulin-2 isoform a precursor
NM_001998.2	NP_001989.2	fibulin-2 isoform b precursor
NM_001165035.2	NP_001158507.1	fibulin-2 isoform a precursor
NM_001004019.2	NP_001004019.1	fibulin-2 isoform a precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	FBLN2	XP_425156.4
Drosophila melanogaster (fruit fly)	CG31999	NP_726551.2
Pan troglodytes (chimpanzee)	FBLN2	XP_003309683.1
Canis lupus familiaris (dog)	FBLN2	XP_541748.2
Mus musculus (house mouse)	Fbln2	NP_032018.2
Rattus norvegicus (Norway rat)	Fbln2	XP_006236985.1
Danio rerio (zebrafish)	fbln2	XP_001341277.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011322	XP_309330.4
Homo sapiens (human)	FBLN2	NP_001158507.1
Bos taurus (cattle)	FBLN2	XP_002697177.1

Gene Ontology
Bibliography

Related articles in PubMed

Comprehensive proteomic characterization of stem cell-derived extracellular matrices.
Ragelle H, Naba A, Larson BL, Zhou F, Priji? M, Whittaker CA, Del Rosario A, Langer R, Hynes RO, Anderson DG
Biomaterials128147-159(2017 06)

Cleavage of Fibulin-2 by the aggrecanases ADAMTS-4 and ADAMTS-5 contributes to the tumorigenic potential of breast cancer cells.
Fontanil T, ?lvarez-Teijeiro S, Villaronga M?, Mohamedi Y, Solares L, Moncada-Pazos A, Vega JA, Garc?a-Su?rez O, P?rez-Basterrechea M, Garc?a-Pedrero JM, Obaya AJ, Cal S
Oncotarget8(8)13716-13729(2017 Feb)

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

Extracellular matrix signatures of human primary metastatic colon cancers and their metastases to liver.
Naba A, Clauser KR, Whittaker CA, Carr SA, Tanabe KK, Hynes RO
BMC cancer14518(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Using in vitro approaches and cultured breast cancer cell lines authors demonstrate that Fibulin-2 is a better substrate for ADAMTS-5 than it is for ADAMTS-4. Fibulin-2 degradation is associated to an enhancement of the invasive potential of T47D, MCF-7 and SK-BR-3 cells.
Title: Cleavage of Fibulin-2 by the aggrecanases ADAMTS-4 and ADAMTS-5 contributes to the tumorigenic potential of breast cancer cells.

levels of fibulin 2 dramatically increased in the retinal pigment epithelium following retinal detachment, suggesting a direct role for fibulin 2 in the re-attachment of the retina to the retinal pigment epithelium.
Title: Fibulin 2, a tyrosine O-sulfated protein, is up-regulated following retinal detachment.

Study identified and validated a novel bona fide target of miR-1, Fibullin-2 (Fbln2), a secreted protein implicated in extracellular matrix remodeling.
Title: Therapeutic cardiac-targeted delivery of miR-1 reverses pressure overload-induced cardiac hypertrophy and attenuates pathological remodeling.

Data provide evidence that FBLN2 short isoform (FBLN2S) has an important tumor-suppressive and anti-angiogenic role in nasopharyngeal carcinoma (NPC).
Title: Anti-angiogenic and tumor-suppressive roles of candidate tumor-suppressor gene, Fibulin-2, in nasopharyngeal carcinoma.

Two variants in the fibulin2 gene are associated with lower systolic blood pressure and decreased risk of hypertension.
Title: Two variants in the fibulin2 gene are associated with lower systolic blood pressure and decreased risk of hypertension.

The following FBLN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FBLN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08593	NM_001004019.2 Latest version!	Homo sapiens fibulin 2 (FBLN2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu08593	NM_001165035.2 Latest version!	Homo sapiens fibulin 2 (FBLN2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu29481	NM_001998.3 Latest version!	Homo sapiens fibulin 2 (FBLN2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu37044	XM_006713026.2 Latest version!	Homo sapiens fibulin 2 (FBLN2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu29481	NM_001998.2	Homo sapiens fibulin 2 (FBLN2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu08593	NM_001165035.1	Homo sapiens fibulin 2 (FBLN2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu08593	NM_001004019.1	Homo sapiens fibulin 2 (FBLN2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FBLN2 ( NM_001004019.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001004019.1 Homo sapiens fibulin 2 (FBLN2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08593
Clone ID Related Accession (Same CDS sequence)	NM_001004019.1 , NM_001165035.1 , NM_001165035.2 , NM_001004019.2
Accession Version	NM_001004019.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	fibulin-2 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB959783.1, BC051690.1, AY130459.1 and AL050095.1. On Nov 22, 2018 this sequence version replaced NM_001004019.1. Transcript Variant: This variant (1) represents use of an alternate promoter and 5' UTR, compared to variant 3, and encodes the longer isoform (a). Both variants 1 and 3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.255294.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000404922.8/ ENSP00000384169.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGCTGC TCTGGGAGCC TGCAGGAGCC TGGCTTGCTC TGGGCCTGGC CCTGGCCCTG 
GGCCCCAGCG TGGCCGCAGC TGCCCCTCGG CAGGACTGCA CGGGCGTGGA GTGCCCGCCG 
CTGGAGAACT GCATTGAGGA GGCGCTGGAG CCGGGTGCCT GCTGTGCCAC GTGTGTGCAG 
CAGGGCTGCG CCTGCGAGGG CTACCAGTAC TATGACTGCC TACAGGGTGG CTTCGTGCGC 
GGCCGCGTGC CCGCCGGTCA GTCCTATTTT GTGGACTTCG GGAGCACTGA GTGCTCCTGC 
CCACCAGGCG GCGGCAAGAT CAGCTGCCAG TTCATGCTGT GCCCGGAGCT GCCGCCCAAC 
TGCATCGAGG CTGTAGTGGT GGCTGACAGC TGCCCACAGT GCGGCCAGGT GGGCTGCGTC 
CACGCGGGCC ACAAGTACGC CGCTGGCCAC ACTGTTCACC TGCCGCCCTG CCGGGCCTGC 
CACTGCCCTG ACGCCGGTGG AGAGCTCATC TGCTACCAGC TCCCCGGTTG CCACGGGAAC 
TTCTCAGATG CCGAGGAGGG TGACCCCGAG CGACACTACG AAGACCCCTA CAGCTATGAC 
CAGGAGGTGG CCGAGGTGGA AGCAGCAACA GCCCTGGGGG GTGAGGTCCA GGCGGGTGCA 
GTCCAGGCAG GCGCAGGGGG CCCCCCAGCT GCTCTGGGAG GTGGGAGTCA GCCACTGTCC 
ACCATCCAGG CACCCCCCTG GCCAGCTGTC CTCCCCAGGC CCACAGCGGC TGCTGCCCTG 
GGTCCCCCAG CCCCAGTGCA GGCCAAAGCT AGGAGAGTGA CCGAGGACAG TGAGGAGGAA 
GAAGAGGAGG AGGAGGAGAG AGAGGAAATG GCTGTCACTG AGCAGCTGGC AGCAGGTGGC 
CACAGGGGGC TGGATGGGCT GCCCACTACA GCCCCAGCTG GACCCAGTCT TCCTATCCAG 
GAGGAGAGGG CAGAAGCTGG GGCAAGGGCA GAAGCTGGGG CAAGGCCTGA AGAGAACCTC 
ATCCTGGATG CCCAAGCCAC GTCCCGCAGC ACTGGGCCGG AGGGCGTGAC GCATGCACCG 
AGCCTGGGCA AGGCTGCTCT CGTCCCAACT CAGGCCGTGC CTGGCTCTCC CAGGGACCCA 
GTCAAGCCCA GCCCCCACAA CATCCTGTCC ACATCACTGC CTGATGCAGC CTGGATCCCA 
CCCACCCGAG AAGTGCCCAG GAAGCCGCAA GTTCTGCCCC ATTCCCACGT GGAGGAGGAC 
ACAGACCCCA ACTCTGTCCA TTCTATCCCC AGAAGTAGCC CTGAAGGCTC CACCAAGGAC 
CTGATCGAGA CTTGCTGCGC AGCCGGACAG CAGTGGGCCA TTGACAATGA CGAGTGCCTG 
GAGATCCCTG AGAGTGGCAC TGAGGACAAC GTCTGCAGGA CAGCCCAGAG GCACTGCTGT 
GTCTCCTACT TGCAGGAGAA GAGCTGCATG GCCGGCGTCC TGGGAGCCAA GGAGGGTGAG 
ACCTGTGGGG CTGAGGACAA CGACAGCTGC GGCATCTCCC TGTACAAGCA ATGCTGTGAC 
TGCTGTGGCC TGGGCCTCCG CGTGCGGGCC GAGGGCCAGT CGTGTGAGTC CAATCCTAAC 
CTGGGCTATC CCTGCAATCA TGTCATGCTC TCCTGCTGTG AGGGTGAAGA GCCTCTCATA 
GTACCTGAGG TTCGCCGACC TCCAGAGCCC GCAGCTGCAC CACGGAGAGT TTCAGAGGCA 
GAGATGGCGG GCCGAGAGGC CCTGTCACTG GGCACAGAGG CCGAGCTGCC GAACAGCCTG 
CCGGGCGATG ACCAGGATGA GTGCCTTCTC CTCCCGGGAG AGCTGTGCCA GCACCTTTGC 
ATCAATACTG TGGGTTCTTA CCACTGTGCC TGCTTTCCTG GCTTCTCACT GCAGGACGAT 
GGCCGCACTT GCCGCCCAGA GGGTCACCCT CCACAGCCGG AAGCCCCACA GGAGCCTGCA 
CTGAAGTCAG AATTTTCCCA GGTGGCCTCT AACACCATCC CGCTGCCACT GCCGCAGCCC 
AATACCTGCA AAGACAATGG ACCCTGCAAG CAGGTGTGCA GCACTGTTGG GGGCTCAGCC 
ATATGCTCCT GTTTTCCCGG CTATGCCATC ATGGCGGATG GCGTGTCCTG TGAAGACCAA 
GACGAGTGCC TGATGGGTGC TCACGATTGT AGCCGGCGAC AGTTCTGTGT GAACACCCTG 
GGATCCTTCT ACTGTGTCAA CCACACAGTG CTCTGTGCCG ATGGCTATAT CCTCAATGCG 
CACAGGAAGT GCGTGGACAT CAACGAGTGT GTGACGGACC TGCACACGTG CAGCCGGGGC 
GAGCACTGTG TGAACACACT GGGCTCCTTC CACTGCTACA AGGCACTCAC CTGTGAGCCA 
GGCTATGCCC TCAAGGATGG CGAGTGCGAA GACGTGGATG AGTGTGCGAT GGGCACGCAC 
ACCTGCCAGC CGGGCTTCTT GTGCCAGAAC ACCAAGGGCT CCTTCTACTG CCAGGCCAGG 
CAGCGCTGCA TGGATGGCTT CCTGCAGGAT CCTGAAGGCA ACTGTGTGGA CATCAACGAG 
TGCACGTCAC TGTCCGAGCC ATGTCGGCCA GGCTTCAGCT GCATCAACAC GGTGGGCTCC 
TACACATGCC AGAGGAACCC GCTGATCTGC GCGCGCGGCT ACCACGCCAG CGATGATGGG 
ACCAAGTGTG TGGACGTGAA TGAGTGTGAG ACAGGTGTGC ACCGCTGCGG TGAGGGCCAA 
GTGTGCCACA ACCTCCCTGG CTCCTACCGC TGTGACTGCA AAGCCGGCTT TCAGCGGGAT 
GCCTTTGGCC GGGGCTGCAT CGACGTGAAT GAGTGCTGGG CCTCGCCAGG CCGCCTGTGC 
CAGCACACGT GTGAGAACAC ACTCGGCTCC TACCGCTGTT CCTGCGCCTC CGGGTTCCTG 
CTAGCAGCGG ACGGCAAGCG CTGTGAAGAC GTGAATGAGT GTGAGGCCCA GCGCTGCAGC 
CAGGAGTGTG CCAACATCTA TGGCTCCTAC CAGTGCTACT GCCGCCAGGG CTACCAGCTG 
GCTGAGGATG GGCACACCTG CACAGACATC GACGAGTGTG CTCAAGGCGC CGGCATCCTC 
TGCACCTTCC GCTGTCTCAA CGTGCCAGGG AGCTACCAGT GTGCATGCCC TGAGCAGGGC 
TACACCATGA CGGCCAACGG GAGGTCCTGC AAGGACGTGG ATGAGTGTGC ACTGGGTACC 
CACAACTGTT CCGAGGCTGA GACCTGCCAC AACATCCAGG GTAGCTTCCG CTGCCTGCGC 
TTCGAGTGTC CTCCCAACTA TGTCCAAGTC TCCAAAACGA AGTGCGAGCG CACCACGTGC 
CATGACTTCC TGGAGTGCCA GAACTCGCCA GCGCGCATCA CGCACTACCA GCTCAACTTC 
CAGACGGGCC TCCTGGTGCC TGCGCATATC TTCCGCATTG GCCCCGCGCC AGCCTTCACG 
GGGGACACCA TCGCCCTGAA CATCATCAAG GGCAATGAGG AGGGCTACTT TGGCACGCGC 
AGGCTCAATG CCTACACGGG TGTGGTCTAC CTGCAGCGGG CCGTGCTGGA GCCCCGGGAC 
TTTGCCCTGG ACGTGGAGAT GAAGCTCTGG AGGCAGGGCT CCGTCACCAC CTTCCTGGCC 
AAGATGCACA TCTTCTTCAC CACCTTTGCC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004019.1
CDS	126..3821
Translation	MVLLWEPAGAWLALGLALALGPSVAAAAPRQDCTGVECPPLENCIEEALEPGACCATCVQQGCACEGYQYYDCLQGGFVRGRVPAGQSYFVDFGSTECSCPPGGGKISCQFMLCPELPPNCIEAVVVADSCPQCGQVGCVHAGHKYAAGHTVHLPPCRACHCPDAGGELICYQLPGCHGNFSDAEEGDPERHYEDPYSYDQEVAEVEAATALGGEVQAGAVQAGAGGPPAALGGGSQPLSTIQAPPWPAVLPRPTAAAALGPPAPVQAKARRVTEDSEEEEEEEEEREEMAVTEQLAAGGHRGLDGLPTTAPAGPSLPIQEERAEAGARAEAGARPEENLILDAQATSRSTGPEGVTHAPSLGKAALVPTQAVPGSPRDPVKPSPHNILSTSLPDAAWIPPTREVPRKPQVLPHSHVEEDTDPNSVHSIPRSSPEGSTKDLIETCCAAGQQWAIDNDECLEIPESGTEDNVCRTAQRHCCVSYLQEKSCMAGVLGAKEGETCGAEDNDSCGISLYKQCCDCCGLGLRVRAEGQSCESNPNLGYPCNHVMLSCCEGEEPLIVPEVRRPPEPAAAPRRVSEAEMAGREALSLGTEAELPNSLPGDDQDECLLLPGELCQHLCINTVGSYHCACFPGFSLQDDGRTCRPEGHPPQPEAPQEPALKSEFSQVASNTIPLPLPQPNTCKDNGPCKQVCSTVGGSAICSCFPGYAIMADGVSCEDQDECLMGAHDCSRRQFCVNTLGSFYCVNHTVLCADGYILNAHRKCVDINECVTDLHTCSRGEHCVNTLGSFHCYKALTCEPGYALKDGECEDVDECAMGTHTCQPGFLCQNTKGSFYCQARQRCMDGFLQDPEGNCVDINECTSLSEPCRPGFSCINTVGSYTCQRNPLICARGYHASDDGTKCVDVNECETGVHRCGEGQVCHNLPGSYRCDCKAGFQRDAFGRGCIDVNECWASPGRLCQHTCENTLGSYRCSCASGFLLAADGKRCEDVNECEAQRCSQECANIYGSYQCYCRQGYQLAEDGHTCTDIDECAQGAGILCTFRCLNVPGSYQCACPEQGYTMTANGRSCKDVDECALGTHNCSEAETCHNIQGSFRCLRFECPPNYVQVSKTKCERTTCHDFLECQNSPARITHYQLNFQTGLLVPAHIFRIGPAPAFTGDTIALNIIKGNEEGYFGTRRLNAYTGVVYLQRAVLEPRDFALDVEMKLWRQGSVTTFLAKMHIFFTTFAL

Target ORF information:

RefSeq Version	NM_001004019.2
Organism	Homo sapiens(human)
Definition	Homo sapiens fibulin 2 (FBLN2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004019.2

ORF Insert Sequence:

ATGGTGCTGC TCTGGGAGCC TGCAGGAGCC TGGCTTGCTC TGGGCCTGGC CCTGGCCCTG 
GGCCCCAGCG TGGCCGCAGC TGCCCCTCGG CAGGACTGCA CGGGCGTGGA GTGCCCGCCG 
CTGGAGAACT GCATTGAGGA GGCGCTGGAG CCGGGTGCCT GCTGTGCCAC GTGTGTGCAG 
CAGGGCTGCG CCTGCGAGGG CTACCAGTAC TATGACTGCC TACAGGGTGG CTTCGTGCGC 
GGCCGCGTGC CCGCCGGTCA GTCCTATTTT GTGGACTTCG GGAGCACTGA GTGCTCCTGC 
CCACCAGGCG GCGGCAAGAT CAGCTGCCAG TTCATGCTGT GCCCGGAGCT GCCGCCCAAC 
TGCATCGAGG CTGTAGTGGT GGCTGACAGC TGCCCACAGT GCGGCCAGGT GGGCTGCGTC 
CACGCGGGCC ACAAGTACGC CGCTGGCCAC ACTGTTCACC TGCCGCCCTG CCGGGCCTGC 
CACTGCCCTG ACGCCGGTGG AGAGCTCATC TGCTACCAGC TCCCCGGTTG CCACGGGAAC 
TTCTCAGATG CCGAGGAGGG TGACCCCGAG CGACACTACG AAGACCCCTA CAGCTATGAC 
CAGGAGGTGG CCGAGGTGGA AGCAGCAACA GCCCTGGGGG GTGAGGTCCA GGCGGGTGCA 
GTCCAGGCAG GCGCAGGGGG CCCCCCAGCT GCTCTGGGAG GTGGGAGTCA GCCACTGTCC 
ACCATCCAGG CACCCCCCTG GCCAGCTGTC CTCCCCAGGC CCACAGCGGC TGCTGCCCTG 
GGTCCCCCAG CCCCAGTGCA GGCCAAAGCT AGGAGAGTGA CCGAGGACAG TGAGGAGGAA 
GAAGAGGAGG AGGAGGAGAG AGAGGAAATG GCTGTCACTG AGCAGCTGGC AGCAGGTGGC 
CACAGGGGGC TGGATGGGCT GCCCACTACA GCCCCAGCTG GACCCAGTCT TCCTATCCAG 
GAGGAGAGGG CAGAAGCTGG GGCAAGGGCA GAAGCTGGGG CAAGGCCTGA AGAGAACCTC 
ATCCTGGATG CCCAAGCCAC GTCCCGCAGC ACTGGGCCGG AGGGCGTGAC GCATGCACCG 
AGCCTGGGCA AGGCTGCTCT CGTCCCAACT CAGGCCGTGC CTGGCTCTCC CAGGGACCCA 
GTCAAGCCCA GCCCCCACAA CATCCTGTCC ACATCACTGC CTGATGCAGC CTGGATCCCA 
CCCACCCGAG AAGTGCCCAG GAAGCCGCAA GTTCTGCCCC ATTCCCACGT GGAGGAGGAC 
ACAGACCCCA ACTCTGTCCA TTCTATCCCC AGAAGTAGCC CTGAAGGCTC CACCAAGGAC 
CTGATCGAGA CTTGCTGCGC AGCCGGACAG CAGTGGGCCA TTGACAATGA CGAGTGCCTG 
GAGATCCCTG AGAGTGGCAC TGAGGACAAC GTCTGCAGGA CAGCCCAGAG GCACTGCTGT 
GTCTCCTACT TGCAGGAGAA GAGCTGCATG GCCGGCGTCC TGGGAGCCAA GGAGGGTGAG 
ACCTGTGGGG CTGAGGACAA CGACAGCTGC GGCATCTCCC TGTACAAGCA ATGCTGTGAC 
TGCTGTGGCC TGGGCCTCCG CGTGCGGGCC GAGGGCCAGT CGTGTGAGTC CAATCCTAAC 
CTGGGCTATC CCTGCAATCA TGTCATGCTC TCCTGCTGTG AGGGTGAAGA GCCTCTCATA 
GTACCTGAGG TTCGCCGACC TCCAGAGCCC GCAGCTGCAC CACGGAGAGT TTCAGAGGCA 
GAGATGGCGG GCCGAGAGGC CCTGTCACTG GGCACAGAGG CCGAGCTGCC GAACAGCCTG 
CCGGGCGATG ACCAGGATGA GTGCCTTCTC CTCCCGGGAG AGCTGTGCCA GCACCTTTGC 
ATCAATACTG TGGGTTCTTA CCACTGTGCC TGCTTTCCTG GCTTCTCACT GCAGGACGAT 
GGCCGCACTT GCCGCCCAGA GGGTCACCCT CCACAGCCGG AAGCCCCACA GGAGCCTGCA 
CTGAAGTCAG AATTTTCCCA GGTGGCCTCT AACACCATCC CGCTGCCACT GCCGCAGCCC 
AATACCTGCA AAGACAATGG ACCCTGCAAG CAGGTGTGCA GCACTGTTGG GGGCTCAGCC 
ATATGCTCCT GTTTTCCCGG CTATGCCATC ATGGCGGATG GCGTGTCCTG TGAAGACCAA 
GACGAGTGCC TGATGGGTGC TCACGATTGT AGCCGGCGAC AGTTCTGTGT GAACACCCTG 
GGATCCTTCT ACTGTGTCAA CCACACAGTG CTCTGTGCCG ATGGCTATAT CCTCAATGCG 
CACAGGAAGT GCGTGGACAT CAACGAGTGT GTGACGGACC TGCACACGTG CAGCCGGGGC 
GAGCACTGTG TGAACACACT GGGCTCCTTC CACTGCTACA AGGCACTCAC CTGTGAGCCA 
GGCTATGCCC TCAAGGATGG CGAGTGCGAA GACGTGGATG AGTGTGCGAT GGGCACGCAC 
ACCTGCCAGC CGGGCTTCTT GTGCCAGAAC ACCAAGGGCT CCTTCTACTG CCAGGCCAGG 
CAGCGCTGCA TGGATGGCTT CCTGCAGGAT CCTGAAGGCA ACTGTGTGGA CATCAACGAG 
TGCACGTCAC TGTCCGAGCC ATGTCGGCCA GGCTTCAGCT GCATCAACAC GGTGGGCTCC 
TACACATGCC AGAGGAACCC GCTGATCTGC GCGCGCGGCT ACCACGCCAG CGATGATGGG 
ACCAAGTGTG TGGACGTGAA TGAGTGTGAG ACAGGTGTGC ACCGCTGCGG TGAGGGCCAA 
GTGTGCCACA ACCTCCCTGG CTCCTACCGC TGTGACTGCA AAGCCGGCTT TCAGCGGGAT 
GCCTTTGGCC GGGGCTGCAT CGACGTGAAT GAGTGCTGGG CCTCGCCAGG CCGCCTGTGC 
CAGCACACGT GTGAGAACAC ACTCGGCTCC TACCGCTGTT CCTGCGCCTC CGGGTTCCTG 
CTAGCAGCGG ACGGCAAGCG CTGTGAAGAC GTGAATGAGT GTGAGGCCCA GCGCTGCAGC 
CAGGAGTGTG CCAACATCTA TGGCTCCTAC CAGTGCTACT GCCGCCAGGG CTACCAGCTG 
GCTGAGGATG GGCACACCTG CACAGACATC GACGAGTGTG CTCAAGGCGC CGGCATCCTC 
TGCACCTTCC GCTGTCTCAA CGTGCCAGGG AGCTACCAGT GTGCATGCCC TGAGCAGGGC 
TACACCATGA CGGCCAACGG GAGGTCCTGC AAGGACGTGG ATGAGTGTGC ACTGGGTACC 
CACAACTGTT CCGAGGCTGA GACCTGCCAC AACATCCAGG GTAGCTTCCG CTGCCTGCGC 
TTCGAGTGTC CTCCCAACTA TGTCCAAGTC TCCAAAACGA AGTGCGAGCG CACCACGTGC 
CATGACTTCC TGGAGTGCCA GAACTCGCCA GCGCGCATCA CGCACTACCA GCTCAACTTC 
CAGACGGGCC TCCTGGTGCC TGCGCATATC TTCCGCATTG GCCCCGCGCC AGCCTTCACG 
GGGGACACCA TCGCCCTGAA CATCATCAAG GGCAATGAGG AGGGCTACTT TGGCACGCGC 
AGGCTCAATG CCTACACGGG TGTGGTCTAC CTGCAGCGGG CCGTGCTGGA GCCCCGGGAC 
TTTGCCCTGG ACGTGGAGAT GAAGCTCTGG AGGCAGGGCT CCGTCACCAC CTTCCTGGCC 
AAGATGCACA TCTTCTTCAC CACCTTTGCC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FBLN2 ( NM_001004019.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001004019.1 Homo sapiens fibulin 2 (FBLN2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08593
Clone ID Related Accession (Same CDS sequence)	NM_001004019.1 , NM_001165035.1 , NM_001165035.2 , NM_001004019.2
Accession Version	NM_001004019.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	fibulin-2 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB959783.1, BC051690.1, AY130459.1 and AL050095.1. On Aug 31, 2013 this sequence version replaced XM_005264948.1. Transcript Variant: This variant (1) represents use of an alternate promoter and 5' UTR, compared to variant 3, and encodes the longer isoform (a). Both variants 1 and 3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCTGC TCTGGGAGCC TGCAGGAGCC TGGCTTGCTC TGGGCCTGGC CCTGGCCCTG 
GGCCCCAGCG TGGCCGCAGC TGCCCCTCGG CAGGACTGCA CGGGCGTGGA GTGCCCGCCG 
CTGGAGAACT GCATTGAGGA GGCGCTGGAG CCGGGTGCCT GCTGTGCCAC GTGTGTGCAG 
CAGGGCTGCG CCTGCGAGGG CTACCAGTAC TATGACTGCC TACAGGGTGG CTTCGTGCGC 
GGCCGCGTGC CCGCCGGTCA GTCCTATTTT GTGGACTTCG GGAGCACTGA GTGCTCCTGC 
CCACCAGGCG GCGGCAAGAT CAGCTGCCAG TTCATGCTGT GCCCGGAGCT GCCGCCCAAC 
TGCATCGAGG CTGTAGTGGT GGCTGACAGC TGCCCACAGT GCGGCCAGGT GGGCTGCGTC 
CACGCGGGCC ACAAGTACGC CGCTGGCCAC ACTGTTCACC TGCCGCCCTG CCGGGCCTGC 
CACTGCCCTG ACGCCGGTGG AGAGCTCATC TGCTACCAGC TCCCCGGTTG CCACGGGAAC 
TTCTCAGATG CCGAGGAGGG TGACCCCGAG CGACACTACG AAGACCCCTA CAGCTATGAC 
CAGGAGGTGG CCGAGGTGGA AGCAGCAACA GCCCTGGGGG GTGAGGTCCA GGCGGGTGCA 
GTCCAGGCAG GCGCAGGGGG CCCCCCAGCT GCTCTGGGAG GTGGGAGTCA GCCACTGTCC 
ACCATCCAGG CACCCCCCTG GCCAGCTGTC CTCCCCAGGC CCACAGCGGC TGCTGCCCTG 
GGTCCCCCAG CCCCAGTGCA GGCCAAAGCT AGGAGAGTGA CCGAGGACAG TGAGGAGGAA 
GAAGAGGAGG AGGAGGAGAG AGAGGAAATG GCTGTCACTG AGCAGCTGGC AGCAGGTGGC 
CACAGGGGGC TGGATGGGCT GCCCACTACA GCCCCAGCTG GACCCAGTCT TCCTATCCAG 
GAGGAGAGGG CAGAAGCTGG GGCAAGGGCA GAAGCTGGGG CAAGGCCTGA AGAGAACCTC 
ATCCTGGATG CCCAAGCCAC GTCCCGCAGC ACTGGGCCGG AGGGCGTGAC GCATGCACCG 
AGCCTGGGCA AGGCTGCTCT CGTCCCAACT CAGGCCGTGC CTGGCTCTCC CAGGGACCCA 
GTCAAGCCCA GCCCCCACAA CATCCTGTCC ACATCACTGC CTGATGCAGC CTGGATCCCA 
CCCACCCGAG AAGTGCCCAG GAAGCCGCAA GTTCTGCCCC ATTCCCACGT GGAGGAGGAC 
ACAGACCCCA ACTCTGTCCA TTCTATCCCC AGAAGTAGCC CTGAAGGCTC CACCAAGGAC 
CTGATCGAGA CTTGCTGCGC AGCCGGACAG CAGTGGGCCA TTGACAATGA CGAGTGCCTG 
GAGATCCCTG AGAGTGGCAC TGAGGACAAC GTCTGCAGGA CAGCCCAGAG GCACTGCTGT 
GTCTCCTACT TGCAGGAGAA GAGCTGCATG GCCGGCGTCC TGGGAGCCAA GGAGGGTGAG 
ACCTGTGGGG CTGAGGACAA CGACAGCTGC GGCATCTCCC TGTACAAGCA ATGCTGTGAC 
TGCTGTGGCC TGGGCCTCCG CGTGCGGGCC GAGGGCCAGT CGTGTGAGTC CAATCCTAAC 
CTGGGCTATC CCTGCAATCA TGTCATGCTC TCCTGCTGTG AGGGTGAAGA GCCTCTCATA 
GTACCTGAGG TTCGCCGACC TCCAGAGCCC GCAGCTGCAC CACGGAGAGT TTCAGAGGCA 
GAGATGGCGG GCCGAGAGGC CCTGTCACTG GGCACAGAGG CCGAGCTGCC GAACAGCCTG 
CCGGGCGATG ACCAGGATGA GTGCCTTCTC CTCCCGGGAG AGCTGTGCCA GCACCTTTGC 
ATCAATACTG TGGGTTCTTA CCACTGTGCC TGCTTTCCTG GCTTCTCACT GCAGGACGAT 
GGCCGCACTT GCCGCCCAGA GGGTCACCCT CCACAGCCGG AAGCCCCACA GGAGCCTGCA 
CTGAAGTCAG AATTTTCCCA GGTGGCCTCT AACACCATCC CGCTGCCACT GCCGCAGCCC 
AATACCTGCA AAGACAATGG ACCCTGCAAG CAGGTGTGCA GCACTGTTGG GGGCTCAGCC 
ATATGCTCCT GTTTTCCCGG CTATGCCATC ATGGCGGATG GCGTGTCCTG TGAAGACCAA 
GACGAGTGCC TGATGGGTGC TCACGATTGT AGCCGGCGAC AGTTCTGTGT GAACACCCTG 
GGATCCTTCT ACTGTGTCAA CCACACAGTG CTCTGTGCCG ATGGCTATAT CCTCAATGCG 
CACAGGAAGT GCGTGGACAT CAACGAGTGT GTGACGGACC TGCACACGTG CAGCCGGGGC 
GAGCACTGTG TGAACACACT GGGCTCCTTC CACTGCTACA AGGCACTCAC CTGTGAGCCA 
GGCTATGCCC TCAAGGATGG CGAGTGCGAA GACGTGGATG AGTGTGCGAT GGGCACGCAC 
ACCTGCCAGC CGGGCTTCTT GTGCCAGAAC ACCAAGGGCT CCTTCTACTG CCAGGCCAGG 
CAGCGCTGCA TGGATGGCTT CCTGCAGGAT CCTGAAGGCA ACTGTGTGGA CATCAACGAG 
TGCACGTCAC TGTCCGAGCC ATGTCGGCCA GGCTTCAGCT GCATCAACAC GGTGGGCTCC 
TACACATGCC AGAGGAACCC GCTGATCTGC GCGCGCGGCT ACCACGCCAG CGATGATGGG 
ACCAAGTGTG TGGACGTGAA TGAGTGTGAG ACAGGTGTGC ACCGCTGCGG TGAGGGCCAA 
GTGTGCCACA ACCTCCCTGG CTCCTACCGC TGTGACTGCA AAGCCGGCTT TCAGCGGGAT 
GCCTTTGGCC GGGGCTGCAT CGACGTGAAT GAGTGCTGGG CCTCGCCAGG CCGCCTGTGC 
CAGCACACGT GTGAGAACAC ACTCGGCTCC TACCGCTGTT CCTGCGCCTC CGGGTTCCTG 
CTAGCAGCGG ACGGCAAGCG CTGTGAAGAC GTGAATGAGT GTGAGGCCCA GCGCTGCAGC 
CAGGAGTGTG CCAACATCTA TGGCTCCTAC CAGTGCTACT GCCGCCAGGG CTACCAGCTG 
GCTGAGGATG GGCACACCTG CACAGACATC GACGAGTGTG CTCAAGGCGC CGGCATCCTC 
TGCACCTTCC GCTGTCTCAA CGTGCCAGGG AGCTACCAGT GTGCATGCCC TGAGCAGGGC 
TACACCATGA CGGCCAACGG GAGGTCCTGC AAGGACGTGG ATGAGTGTGC ACTGGGTACC 
CACAACTGTT CCGAGGCTGA GACCTGCCAC AACATCCAGG GTAGCTTCCG CTGCCTGCGC 
TTCGAGTGTC CTCCCAACTA TGTCCAAGTC TCCAAAACGA AGTGCGAGCG CACCACGTGC 
CATGACTTCC TGGAGTGCCA GAACTCGCCA GCGCGCATCA CGCACTACCA GCTCAACTTC 
CAGACGGGCC TCCTGGTGCC TGCGCATATC TTCCGCATTG GCCCCGCGCC AGCCTTCACG 
GGGGACACCA TCGCCCTGAA CATCATCAAG GGCAATGAGG AGGGCTACTT TGGCACGCGC 
AGGCTCAATG CCTACACGGG TGTGGTCTAC CTGCAGCGGG CCGTGCTGGA GCCCCGGGAC 
TTTGCCCTGG ACGTGGAGAT GAAGCTCTGG AGGCAGGGCT CCGTCACCAC CTTCCTGGCC 
AAGATGCACA TCTTCTTCAC CACCTTTGCC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004019.1
CDS	126..3821
Translation	MVLLWEPAGAWLALGLALALGPSVAAAAPRQDCTGVECPPLENCIEEALEPGACCATCVQQGCACEGYQYYDCLQGGFVRGRVPAGQSYFVDFGSTECSCPPGGGKISCQFMLCPELPPNCIEAVVVADSCPQCGQVGCVHAGHKYAAGHTVHLPPCRACHCPDAGGELICYQLPGCHGNFSDAEEGDPERHYEDPYSYDQEVAEVEAATALGGEVQAGAVQAGAGGPPAALGGGSQPLSTIQAPPWPAVLPRPTAAAALGPPAPVQAKARRVTEDSEEEEEEEEEREEMAVTEQLAAGGHRGLDGLPTTAPAGPSLPIQEERAEAGARAEAGARPEENLILDAQATSRSTGPEGVTHAPSLGKAALVPTQAVPGSPRDPVKPSPHNILSTSLPDAAWIPPTREVPRKPQVLPHSHVEEDTDPNSVHSIPRSSPEGSTKDLIETCCAAGQQWAIDNDECLEIPESGTEDNVCRTAQRHCCVSYLQEKSCMAGVLGAKEGETCGAEDNDSCGISLYKQCCDCCGLGLRVRAEGQSCESNPNLGYPCNHVMLSCCEGEEPLIVPEVRRPPEPAAAPRRVSEAEMAGREALSLGTEAELPNSLPGDDQDECLLLPGELCQHLCINTVGSYHCACFPGFSLQDDGRTCRPEGHPPQPEAPQEPALKSEFSQVASNTIPLPLPQPNTCKDNGPCKQVCSTVGGSAICSCFPGYAIMADGVSCEDQDECLMGAHDCSRRQFCVNTLGSFYCVNHTVLCADGYILNAHRKCVDINECVTDLHTCSRGEHCVNTLGSFHCYKALTCEPGYALKDGECEDVDECAMGTHTCQPGFLCQNTKGSFYCQARQRCMDGFLQDPEGNCVDINECTSLSEPCRPGFSCINTVGSYTCQRNPLICARGYHASDDGTKCVDVNECETGVHRCGEGQVCHNLPGSYRCDCKAGFQRDAFGRGCIDVNECWASPGRLCQHTCENTLGSYRCSCASGFLLAADGKRCEDVNECEAQRCSQECANIYGSYQCYCRQGYQLAEDGHTCTDIDECAQGAGILCTFRCLNVPGSYQCACPEQGYTMTANGRSCKDVDECALGTHNCSEAETCHNIQGSFRCLRFECPPNYVQVSKTKCERTTCHDFLECQNSPARITHYQLNFQTGLLVPAHIFRIGPAPAFTGDTIALNIIKGNEEGYFGTRRLNAYTGVVYLQRAVLEPRDFALDVEMKLWRQGSVTTFLAKMHIFFTTFAL

Target ORF information:

RefSeq Version	NM_001004019.1
Organism	Homo sapiens(human)
Definition	Homo sapiens fibulin 2 (FBLN2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004019.1

ORF Insert Sequence:

ATGGTGCTGC TCTGGGAGCC TGCAGGAGCC TGGCTTGCTC TGGGCCTGGC CCTGGCCCTG 
GGCCCCAGCG TGGCCGCAGC TGCCCCTCGG CAGGACTGCA CGGGCGTGGA GTGCCCGCCG 
CTGGAGAACT GCATTGAGGA GGCGCTGGAG CCGGGTGCCT GCTGTGCCAC GTGTGTGCAG 
CAGGGCTGCG CCTGCGAGGG CTACCAGTAC TATGACTGCC TACAGGGTGG CTTCGTGCGC 
GGCCGCGTGC CCGCCGGTCA GTCCTATTTT GTGGACTTCG GGAGCACTGA GTGCTCCTGC 
CCACCAGGCG GCGGCAAGAT CAGCTGCCAG TTCATGCTGT GCCCGGAGCT GCCGCCCAAC 
TGCATCGAGG CTGTAGTGGT GGCTGACAGC TGCCCACAGT GCGGCCAGGT GGGCTGCGTC 
CACGCGGGCC ACAAGTACGC CGCTGGCCAC ACTGTTCACC TGCCGCCCTG CCGGGCCTGC 
CACTGCCCTG ACGCCGGTGG AGAGCTCATC TGCTACCAGC TCCCCGGTTG CCACGGGAAC 
TTCTCAGATG CCGAGGAGGG TGACCCCGAG CGACACTACG AAGACCCCTA CAGCTATGAC 
CAGGAGGTGG CCGAGGTGGA AGCAGCAACA GCCCTGGGGG GTGAGGTCCA GGCGGGTGCA 
GTCCAGGCAG GCGCAGGGGG CCCCCCAGCT GCTCTGGGAG GTGGGAGTCA GCCACTGTCC 
ACCATCCAGG CACCCCCCTG GCCAGCTGTC CTCCCCAGGC CCACAGCGGC TGCTGCCCTG 
GGTCCCCCAG CCCCAGTGCA GGCCAAAGCT AGGAGAGTGA CCGAGGACAG TGAGGAGGAA 
GAAGAGGAGG AGGAGGAGAG AGAGGAAATG GCTGTCACTG AGCAGCTGGC AGCAGGTGGC 
CACAGGGGGC TGGATGGGCT GCCCACTACA GCCCCAGCTG GACCCAGTCT TCCTATCCAG 
GAGGAGAGGG CAGAAGCTGG GGCAAGGGCA GAAGCTGGGG CAAGGCCTGA AGAGAACCTC 
ATCCTGGATG CCCAAGCCAC GTCCCGCAGC ACTGGGCCGG AGGGCGTGAC GCATGCACCG 
AGCCTGGGCA AGGCTGCTCT CGTCCCAACT CAGGCCGTGC CTGGCTCTCC CAGGGACCCA 
GTCAAGCCCA GCCCCCACAA CATCCTGTCC ACATCACTGC CTGATGCAGC CTGGATCCCA 
CCCACCCGAG AAGTGCCCAG GAAGCCGCAA GTTCTGCCCC ATTCCCACGT GGAGGAGGAC 
ACAGACCCCA ACTCTGTCCA TTCTATCCCC AGAAGTAGCC CTGAAGGCTC CACCAAGGAC 
CTGATCGAGA CTTGCTGCGC AGCCGGACAG CAGTGGGCCA TTGACAATGA CGAGTGCCTG 
GAGATCCCTG AGAGTGGCAC TGAGGACAAC GTCTGCAGGA CAGCCCAGAG GCACTGCTGT 
GTCTCCTACT TGCAGGAGAA GAGCTGCATG GCCGGCGTCC TGGGAGCCAA GGAGGGTGAG 
ACCTGTGGGG CTGAGGACAA CGACAGCTGC GGCATCTCCC TGTACAAGCA ATGCTGTGAC 
TGCTGTGGCC TGGGCCTCCG CGTGCGGGCC GAGGGCCAGT CGTGTGAGTC CAATCCTAAC 
CTGGGCTATC CCTGCAATCA TGTCATGCTC TCCTGCTGTG AGGGTGAAGA GCCTCTCATA 
GTACCTGAGG TTCGCCGACC TCCAGAGCCC GCAGCTGCAC CACGGAGAGT TTCAGAGGCA 
GAGATGGCGG GCCGAGAGGC CCTGTCACTG GGCACAGAGG CCGAGCTGCC GAACAGCCTG 
CCGGGCGATG ACCAGGATGA GTGCCTTCTC CTCCCGGGAG AGCTGTGCCA GCACCTTTGC 
ATCAATACTG TGGGTTCTTA CCACTGTGCC TGCTTTCCTG GCTTCTCACT GCAGGACGAT 
GGCCGCACTT GCCGCCCAGA GGGTCACCCT CCACAGCCGG AAGCCCCACA GGAGCCTGCA 
CTGAAGTCAG AATTTTCCCA GGTGGCCTCT AACACCATCC CGCTGCCACT GCCGCAGCCC 
AATACCTGCA AAGACAATGG ACCCTGCAAG CAGGTGTGCA GCACTGTTGG GGGCTCAGCC 
ATATGCTCCT GTTTTCCCGG CTATGCCATC ATGGCGGATG GCGTGTCCTG TGAAGACCAA 
GACGAGTGCC TGATGGGTGC TCACGATTGT AGCCGGCGAC AGTTCTGTGT GAACACCCTG 
GGATCCTTCT ACTGTGTCAA CCACACAGTG CTCTGTGCCG ATGGCTATAT CCTCAATGCG 
CACAGGAAGT GCGTGGACAT CAACGAGTGT GTGACGGACC TGCACACGTG CAGCCGGGGC 
GAGCACTGTG TGAACACACT GGGCTCCTTC CACTGCTACA AGGCACTCAC CTGTGAGCCA 
GGCTATGCCC TCAAGGATGG CGAGTGCGAA GACGTGGATG AGTGTGCGAT GGGCACGCAC 
ACCTGCCAGC CGGGCTTCTT GTGCCAGAAC ACCAAGGGCT CCTTCTACTG CCAGGCCAGG 
CAGCGCTGCA TGGATGGCTT CCTGCAGGAT CCTGAAGGCA ACTGTGTGGA CATCAACGAG 
TGCACGTCAC TGTCCGAGCC ATGTCGGCCA GGCTTCAGCT GCATCAACAC GGTGGGCTCC 
TACACATGCC AGAGGAACCC GCTGATCTGC GCGCGCGGCT ACCACGCCAG CGATGATGGG 
ACCAAGTGTG TGGACGTGAA TGAGTGTGAG ACAGGTGTGC ACCGCTGCGG TGAGGGCCAA 
GTGTGCCACA ACCTCCCTGG CTCCTACCGC TGTGACTGCA AAGCCGGCTT TCAGCGGGAT 
GCCTTTGGCC GGGGCTGCAT CGACGTGAAT GAGTGCTGGG CCTCGCCAGG CCGCCTGTGC 
CAGCACACGT GTGAGAACAC ACTCGGCTCC TACCGCTGTT CCTGCGCCTC CGGGTTCCTG 
CTAGCAGCGG ACGGCAAGCG CTGTGAAGAC GTGAATGAGT GTGAGGCCCA GCGCTGCAGC 
CAGGAGTGTG CCAACATCTA TGGCTCCTAC CAGTGCTACT GCCGCCAGGG CTACCAGCTG 
GCTGAGGATG GGCACACCTG CACAGACATC GACGAGTGTG CTCAAGGCGC CGGCATCCTC 
TGCACCTTCC GCTGTCTCAA CGTGCCAGGG AGCTACCAGT GTGCATGCCC TGAGCAGGGC 
TACACCATGA CGGCCAACGG GAGGTCCTGC AAGGACGTGG ATGAGTGTGC ACTGGGTACC 
CACAACTGTT CCGAGGCTGA GACCTGCCAC AACATCCAGG GTAGCTTCCG CTGCCTGCGC 
TTCGAGTGTC CTCCCAACTA TGTCCAAGTC TCCAAAACGA AGTGCGAGCG CACCACGTGC 
CATGACTTCC TGGAGTGCCA GAACTCGCCA GCGCGCATCA CGCACTACCA GCTCAACTTC 
CAGACGGGCC TCCTGGTGCC TGCGCATATC TTCCGCATTG GCCCCGCGCC AGCCTTCACG 
GGGGACACCA TCGCCCTGAA CATCATCAAG GGCAATGAGG AGGGCTACTT TGGCACGCGC 
AGGCTCAATG CCTACACGGG TGTGGTCTAC CTGCAGCGGG CCGTGCTGGA GCCCCGGGAC 
TTTGCCCTGG ACGTGGAGAT GAAGCTCTGG AGGCAGGGCT CCGTCACCAC CTTCCTGGCC 
AAGATGCACA TCTTCTTCAC CACCTTTGCC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FBLN2 ( NM_001165035.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158507.1 Homo sapiens fibulin 2 (FBLN2), transcript variant 3, mRNA.

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CloneID	OHu08593
Clone ID Related Accession (Same CDS sequence)	NM_001004019.1 , NM_001165035.1 , NM_001165035.2 , NM_001004019.2
Accession Version	NM_001165035.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Homo sapiens(human)
Product	fibulin-2 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC090509.2, AK304827.1, AL050095.1, BC051690.1 and AY130459.1. Transcript Variant: This variant (3) represents the longest transcript, and encodes the longer isoform (a). Both variants 1 and 3 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304827.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCTGC TCTGGGAGCC TGCAGGAGCC TGGCTTGCTC TGGGCCTGGC CCTGGCCCTG 
GGCCCCAGCG TGGCCGCAGC TGCCCCTCGG CAGGACTGCA CGGGCGTGGA GTGCCCGCCG 
CTGGAGAACT GCATTGAGGA GGCGCTGGAG CCGGGTGCCT GCTGTGCCAC GTGTGTGCAG 
CAGGGCTGCG CCTGCGAGGG CTACCAGTAC TATGACTGCC TACAGGGTGG CTTCGTGCGC 
GGCCGCGTGC CCGCCGGTCA GTCCTATTTT GTGGACTTCG GGAGCACTGA GTGCTCCTGC 
CCACCAGGCG GCGGCAAGAT CAGCTGCCAG TTCATGCTGT GCCCGGAGCT GCCGCCCAAC 
TGCATCGAGG CTGTAGTGGT GGCTGACAGC TGCCCACAGT GCGGCCAGGT GGGCTGCGTC 
CACGCGGGCC ACAAGTACGC CGCTGGCCAC ACTGTTCACC TGCCGCCCTG CCGGGCCTGC 
CACTGCCCTG ACGCCGGTGG AGAGCTCATC TGCTACCAGC TCCCCGGTTG CCACGGGAAC 
TTCTCAGATG CCGAGGAGGG TGACCCCGAG CGACACTACG AAGACCCCTA CAGCTATGAC 
CAGGAGGTGG CCGAGGTGGA AGCAGCAACA GCCCTGGGGG GTGAGGTCCA GGCGGGTGCA 
GTCCAGGCAG GCGCAGGGGG CCCCCCAGCT GCTCTGGGAG GTGGGAGTCA GCCACTGTCC 
ACCATCCAGG CACCCCCCTG GCCAGCTGTC CTCCCCAGGC CCACAGCGGC TGCTGCCCTG 
GGTCCCCCAG CCCCAGTGCA GGCCAAAGCT AGGAGAGTGA CCGAGGACAG TGAGGAGGAA 
GAAGAGGAGG AGGAGGAGAG AGAGGAAATG GCTGTCACTG AGCAGCTGGC AGCAGGTGGC 
CACAGGGGGC TGGATGGGCT GCCCACTACA GCCCCAGCTG GACCCAGTCT TCCTATCCAG 
GAGGAGAGGG CAGAAGCTGG GGCAAGGGCA GAAGCTGGGG CAAGGCCTGA AGAGAACCTC 
ATCCTGGATG CCCAAGCCAC GTCCCGCAGC ACTGGGCCGG AGGGCGTGAC GCATGCACCG 
AGCCTGGGCA AGGCTGCTCT CGTCCCAACT CAGGCCGTGC CTGGCTCTCC CAGGGACCCA 
GTCAAGCCCA GCCCCCACAA CATCCTGTCC ACATCACTGC CTGATGCAGC CTGGATCCCA 
CCCACCCGAG AAGTGCCCAG GAAGCCGCAA GTTCTGCCCC ATTCCCACGT GGAGGAGGAC 
ACAGACCCCA ACTCTGTCCA TTCTATCCCC AGAAGTAGCC CTGAAGGCTC CACCAAGGAC 
CTGATCGAGA CTTGCTGCGC AGCCGGACAG CAGTGGGCCA TTGACAATGA CGAGTGCCTG 
GAGATCCCTG AGAGTGGCAC TGAGGACAAC GTCTGCAGGA CAGCCCAGAG GCACTGCTGT 
GTCTCCTACT TGCAGGAGAA GAGCTGCATG GCCGGCGTCC TGGGAGCCAA GGAGGGTGAG 
ACCTGTGGGG CTGAGGACAA CGACAGCTGC GGCATCTCCC TGTACAAGCA ATGCTGTGAC 
TGCTGTGGCC TGGGCCTCCG CGTGCGGGCC GAGGGCCAGT CGTGTGAGTC CAATCCTAAC 
CTGGGCTATC CCTGCAATCA TGTCATGCTC TCCTGCTGTG AGGGTGAAGA GCCTCTCATA 
GTACCTGAGG TTCGCCGACC TCCAGAGCCC GCAGCTGCAC CACGGAGAGT TTCAGAGGCA 
GAGATGGCGG GCCGAGAGGC CCTGTCACTG GGCACAGAGG CCGAGCTGCC GAACAGCCTG 
CCGGGCGATG ACCAGGATGA GTGCCTTCTC CTCCCGGGAG AGCTGTGCCA GCACCTTTGC 
ATCAATACTG TGGGTTCTTA CCACTGTGCC TGCTTTCCTG GCTTCTCACT GCAGGACGAT 
GGCCGCACTT GCCGCCCAGA GGGTCACCCT CCACAGCCGG AAGCCCCACA GGAGCCTGCA 
CTGAAGTCAG AATTTTCCCA GGTGGCCTCT AACACCATCC CGCTGCCACT GCCGCAGCCC 
AATACCTGCA AAGACAATGG ACCCTGCAAG CAGGTGTGCA GCACTGTTGG GGGCTCAGCC 
ATATGCTCCT GTTTTCCCGG CTATGCCATC ATGGCGGATG GCGTGTCCTG TGAAGACCAA 
GACGAGTGCC TGATGGGTGC TCACGATTGT AGCCGGCGAC AGTTCTGTGT GAACACCCTG 
GGATCCTTCT ACTGTGTCAA CCACACAGTG CTCTGTGCCG ATGGCTATAT CCTCAATGCG 
CACAGGAAGT GCGTGGACAT CAACGAGTGT GTGACGGACC TGCACACGTG CAGCCGGGGC 
GAGCACTGTG TGAACACACT GGGCTCCTTC CACTGCTACA AGGCACTCAC CTGTGAGCCA 
GGCTATGCCC TCAAGGATGG CGAGTGCGAA GACGTGGATG AGTGTGCGAT GGGCACGCAC 
ACCTGCCAGC CGGGCTTCTT GTGCCAGAAC ACCAAGGGCT CCTTCTACTG CCAGGCCAGG 
CAGCGCTGCA TGGATGGCTT CCTGCAGGAT CCTGAAGGCA ACTGTGTGGA CATCAACGAG 
TGCACGTCAC TGTCCGAGCC ATGTCGGCCA GGCTTCAGCT GCATCAACAC GGTGGGCTCC 
TACACATGCC AGAGGAACCC GCTGATCTGC GCGCGCGGCT ACCACGCCAG CGATGATGGG 
ACCAAGTGTG TGGACGTGAA TGAGTGTGAG ACAGGTGTGC ACCGCTGCGG TGAGGGCCAA 
GTGTGCCACA ACCTCCCTGG CTCCTACCGC TGTGACTGCA AAGCCGGCTT TCAGCGGGAT 
GCCTTTGGCC GGGGCTGCAT CGACGTGAAT GAGTGCTGGG CCTCGCCAGG CCGCCTGTGC 
CAGCACACGT GTGAGAACAC ACTCGGCTCC TACCGCTGTT CCTGCGCCTC CGGGTTCCTG 
CTAGCAGCGG ACGGCAAGCG CTGTGAAGAC GTGAATGAGT GTGAGGCCCA GCGCTGCAGC 
CAGGAGTGTG CCAACATCTA TGGCTCCTAC CAGTGCTACT GCCGCCAGGG CTACCAGCTG 
GCTGAGGATG GGCACACCTG CACAGACATC GACGAGTGTG CTCAAGGCGC CGGCATCCTC 
TGCACCTTCC GCTGTCTCAA CGTGCCAGGG AGCTACCAGT GTGCATGCCC TGAGCAGGGC 
TACACCATGA CGGCCAACGG GAGGTCCTGC AAGGACGTGG ATGAGTGTGC ACTGGGTACC 
CACAACTGTT CCGAGGCTGA GACCTGCCAC AACATCCAGG GTAGCTTCCG CTGCCTGCGC 
TTCGAGTGTC CTCCCAACTA TGTCCAAGTC TCCAAAACGA AGTGCGAGCG CACCACGTGC 
CATGACTTCC TGGAGTGCCA GAACTCGCCA GCGCGCATCA CGCACTACCA GCTCAACTTC 
CAGACGGGCC TCCTGGTGCC TGCGCATATC TTCCGCATTG GCCCCGCGCC AGCCTTCACG 
GGGGACACCA TCGCCCTGAA CATCATCAAG GGCAATGAGG AGGGCTACTT TGGCACGCGC 
AGGCTCAATG CCTACACGGG TGTGGTCTAC CTGCAGCGGG CCGTGCTGGA GCCCCGGGAC 
TTTGCCCTGG ACGTGGAGAT GAAGCTCTGG AGGCAGGGCT CCGTCACCAC CTTCCTGGCC 
AAGATGCACA TCTTCTTCAC CACCTTTGCC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158507.1
CDS	383..4078
Translation	MVLLWEPAGAWLALGLALALGPSVAAAAPRQDCTGVECPPLENCIEEALEPGACCATCVQQGCACEGYQYYDCLQGGFVRGRVPAGQSYFVDFGSTECSCPPGGGKISCQFMLCPELPPNCIEAVVVADSCPQCGQVGCVHAGHKYAAGHTVHLPPCRACHCPDAGGELICYQLPGCHGNFSDAEEGDPERHYEDPYSYDQEVAEVEAATALGGEVQAGAVQAGAGGPPAALGGGSQPLSTIQAPPWPAVLPRPTAAAALGPPAPVQAKARRVTEDSEEEEEEEEEREEMAVTEQLAAGGHRGLDGLPTTAPAGPSLPIQEERAEAGARAEAGARPEENLILDAQATSRSTGPEGVTHAPSLGKAALVPTQAVPGSPRDPVKPSPHNILSTSLPDAAWIPPTREVPRKPQVLPHSHVEEDTDPNSVHSIPRSSPEGSTKDLIETCCAAGQQWAIDNDECLEIPESGTEDNVCRTAQRHCCVSYLQEKSCMAGVLGAKEGETCGAEDNDSCGISLYKQCCDCCGLGLRVRAEGQSCESNPNLGYPCNHVMLSCCEGEEPLIVPEVRRPPEPAAAPRRVSEAEMAGREALSLGTEAELPNSLPGDDQDECLLLPGELCQHLCINTVGSYHCACFPGFSLQDDGRTCRPEGHPPQPEAPQEPALKSEFSQVASNTIPLPLPQPNTCKDNGPCKQVCSTVGGSAICSCFPGYAIMADGVSCEDQDECLMGAHDCSRRQFCVNTLGSFYCVNHTVLCADGYILNAHRKCVDINECVTDLHTCSRGEHCVNTLGSFHCYKALTCEPGYALKDGECEDVDECAMGTHTCQPGFLCQNTKGSFYCQARQRCMDGFLQDPEGNCVDINECTSLSEPCRPGFSCINTVGSYTCQRNPLICARGYHASDDGTKCVDVNECETGVHRCGEGQVCHNLPGSYRCDCKAGFQRDAFGRGCIDVNECWASPGRLCQHTCENTLGSYRCSCASGFLLAADGKRCEDVNECEAQRCSQECANIYGSYQCYCRQGYQLAEDGHTCTDIDECAQGAGILCTFRCLNVPGSYQCACPEQGYTMTANGRSCKDVDECALGTHNCSEAETCHNIQGSFRCLRFECPPNYVQVSKTKCERTTCHDFLECQNSPARITHYQLNFQTGLLVPAHIFRIGPAPAFTGDTIALNIIKGNEEGYFGTRRLNAYTGVVYLQRAVLEPRDFALDVEMKLWRQGSVTTFLAKMHIFFTTFAL

Target ORF information:

RefSeq Version	NM_001165035.1
Organism	Homo sapiens(human)
Definition	Homo sapiens fibulin 2 (FBLN2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165035.1

ORF Insert Sequence:

ATGGTGCTGC TCTGGGAGCC TGCAGGAGCC TGGCTTGCTC TGGGCCTGGC CCTGGCCCTG 
GGCCCCAGCG TGGCCGCAGC TGCCCCTCGG CAGGACTGCA CGGGCGTGGA GTGCCCGCCG 
CTGGAGAACT GCATTGAGGA GGCGCTGGAG CCGGGTGCCT GCTGTGCCAC GTGTGTGCAG 
CAGGGCTGCG CCTGCGAGGG CTACCAGTAC TATGACTGCC TACAGGGTGG CTTCGTGCGC 
GGCCGCGTGC CCGCCGGTCA GTCCTATTTT GTGGACTTCG GGAGCACTGA GTGCTCCTGC 
CCACCAGGCG GCGGCAAGAT CAGCTGCCAG TTCATGCTGT GCCCGGAGCT GCCGCCCAAC 
TGCATCGAGG CTGTAGTGGT GGCTGACAGC TGCCCACAGT GCGGCCAGGT GGGCTGCGTC 
CACGCGGGCC ACAAGTACGC CGCTGGCCAC ACTGTTCACC TGCCGCCCTG CCGGGCCTGC 
CACTGCCCTG ACGCCGGTGG AGAGCTCATC TGCTACCAGC TCCCCGGTTG CCACGGGAAC 
TTCTCAGATG CCGAGGAGGG TGACCCCGAG CGACACTACG AAGACCCCTA CAGCTATGAC 
CAGGAGGTGG CCGAGGTGGA AGCAGCAACA GCCCTGGGGG GTGAGGTCCA GGCGGGTGCA 
GTCCAGGCAG GCGCAGGGGG CCCCCCAGCT GCTCTGGGAG GTGGGAGTCA GCCACTGTCC 
ACCATCCAGG CACCCCCCTG GCCAGCTGTC CTCCCCAGGC CCACAGCGGC TGCTGCCCTG 
GGTCCCCCAG CCCCAGTGCA GGCCAAAGCT AGGAGAGTGA CCGAGGACAG TGAGGAGGAA 
GAAGAGGAGG AGGAGGAGAG AGAGGAAATG GCTGTCACTG AGCAGCTGGC AGCAGGTGGC 
CACAGGGGGC TGGATGGGCT GCCCACTACA GCCCCAGCTG GACCCAGTCT TCCTATCCAG 
GAGGAGAGGG CAGAAGCTGG GGCAAGGGCA GAAGCTGGGG CAAGGCCTGA AGAGAACCTC 
ATCCTGGATG CCCAAGCCAC GTCCCGCAGC ACTGGGCCGG AGGGCGTGAC GCATGCACCG 
AGCCTGGGCA AGGCTGCTCT CGTCCCAACT CAGGCCGTGC CTGGCTCTCC CAGGGACCCA 
GTCAAGCCCA GCCCCCACAA CATCCTGTCC ACATCACTGC CTGATGCAGC CTGGATCCCA 
CCCACCCGAG AAGTGCCCAG GAAGCCGCAA GTTCTGCCCC ATTCCCACGT GGAGGAGGAC 
ACAGACCCCA ACTCTGTCCA TTCTATCCCC AGAAGTAGCC CTGAAGGCTC CACCAAGGAC 
CTGATCGAGA CTTGCTGCGC AGCCGGACAG CAGTGGGCCA TTGACAATGA CGAGTGCCTG 
GAGATCCCTG AGAGTGGCAC TGAGGACAAC GTCTGCAGGA CAGCCCAGAG GCACTGCTGT 
GTCTCCTACT TGCAGGAGAA GAGCTGCATG GCCGGCGTCC TGGGAGCCAA GGAGGGTGAG 
ACCTGTGGGG CTGAGGACAA CGACAGCTGC GGCATCTCCC TGTACAAGCA ATGCTGTGAC 
TGCTGTGGCC TGGGCCTCCG CGTGCGGGCC GAGGGCCAGT CGTGTGAGTC CAATCCTAAC 
CTGGGCTATC CCTGCAATCA TGTCATGCTC TCCTGCTGTG AGGGTGAAGA GCCTCTCATA 
GTACCTGAGG TTCGCCGACC TCCAGAGCCC GCAGCTGCAC CACGGAGAGT TTCAGAGGCA 
GAGATGGCGG GCCGAGAGGC CCTGTCACTG GGCACAGAGG CCGAGCTGCC GAACAGCCTG 
CCGGGCGATG ACCAGGATGA GTGCCTTCTC CTCCCGGGAG AGCTGTGCCA GCACCTTTGC 
ATCAATACTG TGGGTTCTTA CCACTGTGCC TGCTTTCCTG GCTTCTCACT GCAGGACGAT 
GGCCGCACTT GCCGCCCAGA GGGTCACCCT CCACAGCCGG AAGCCCCACA GGAGCCTGCA 
CTGAAGTCAG AATTTTCCCA GGTGGCCTCT AACACCATCC CGCTGCCACT GCCGCAGCCC 
AATACCTGCA AAGACAATGG ACCCTGCAAG CAGGTGTGCA GCACTGTTGG GGGCTCAGCC 
ATATGCTCCT GTTTTCCCGG CTATGCCATC ATGGCGGATG GCGTGTCCTG TGAAGACCAA 
GACGAGTGCC TGATGGGTGC TCACGATTGT AGCCGGCGAC AGTTCTGTGT GAACACCCTG 
GGATCCTTCT ACTGTGTCAA CCACACAGTG CTCTGTGCCG ATGGCTATAT CCTCAATGCG 
CACAGGAAGT GCGTGGACAT CAACGAGTGT GTGACGGACC TGCACACGTG CAGCCGGGGC 
GAGCACTGTG TGAACACACT GGGCTCCTTC CACTGCTACA AGGCACTCAC CTGTGAGCCA 
GGCTATGCCC TCAAGGATGG CGAGTGCGAA GACGTGGATG AGTGTGCGAT GGGCACGCAC 
ACCTGCCAGC CGGGCTTCTT GTGCCAGAAC ACCAAGGGCT CCTTCTACTG CCAGGCCAGG 
CAGCGCTGCA TGGATGGCTT CCTGCAGGAT CCTGAAGGCA ACTGTGTGGA CATCAACGAG 
TGCACGTCAC TGTCCGAGCC ATGTCGGCCA GGCTTCAGCT GCATCAACAC GGTGGGCTCC 
TACACATGCC AGAGGAACCC GCTGATCTGC GCGCGCGGCT ACCACGCCAG CGATGATGGG 
ACCAAGTGTG TGGACGTGAA TGAGTGTGAG ACAGGTGTGC ACCGCTGCGG TGAGGGCCAA 
GTGTGCCACA ACCTCCCTGG CTCCTACCGC TGTGACTGCA AAGCCGGCTT TCAGCGGGAT 
GCCTTTGGCC GGGGCTGCAT CGACGTGAAT GAGTGCTGGG CCTCGCCAGG CCGCCTGTGC 
CAGCACACGT GTGAGAACAC ACTCGGCTCC TACCGCTGTT CCTGCGCCTC CGGGTTCCTG 
CTAGCAGCGG ACGGCAAGCG CTGTGAAGAC GTGAATGAGT GTGAGGCCCA GCGCTGCAGC 
CAGGAGTGTG CCAACATCTA TGGCTCCTAC CAGTGCTACT GCCGCCAGGG CTACCAGCTG 
GCTGAGGATG GGCACACCTG CACAGACATC GACGAGTGTG CTCAAGGCGC CGGCATCCTC 
TGCACCTTCC GCTGTCTCAA CGTGCCAGGG AGCTACCAGT GTGCATGCCC TGAGCAGGGC 
TACACCATGA CGGCCAACGG GAGGTCCTGC AAGGACGTGG ATGAGTGTGC ACTGGGTACC 
CACAACTGTT CCGAGGCTGA GACCTGCCAC AACATCCAGG GTAGCTTCCG CTGCCTGCGC 
TTCGAGTGTC CTCCCAACTA TGTCCAAGTC TCCAAAACGA AGTGCGAGCG CACCACGTGC 
CATGACTTCC TGGAGTGCCA GAACTCGCCA GCGCGCATCA CGCACTACCA GCTCAACTTC 
CAGACGGGCC TCCTGGTGCC TGCGCATATC TTCCGCATTG GCCCCGCGCC AGCCTTCACG 
GGGGACACCA TCGCCCTGAA CATCATCAAG GGCAATGAGG AGGGCTACTT TGGCACGCGC 
AGGCTCAATG CCTACACGGG TGTGGTCTAC CTGCAGCGGG CCGTGCTGGA GCCCCGGGAC 
TTTGCCCTGG ACGTGGAGAT GAAGCTCTGG AGGCAGGGCT CCGTCACCAC CTTCCTGGCC 
AAGATGCACA TCTTCTTCAC CACCTTTGCC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FBLN2 ( NM_001165035.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158507.1 Homo sapiens fibulin 2 (FBLN2), transcript variant 3, mRNA.

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CloneID	OHu08593
Clone ID Related Accession (Same CDS sequence)	NM_001004019.1 , NM_001165035.1 , NM_001165035.2 , NM_001004019.2
Accession Version	NM_001165035.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	fibulin-2 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC090509.2, AK304827.1, AL050095.1 and BC051690.1. On May 31, 2019 this sequence version replaced NM_001165035.1. Transcript Variant: This variant (3) represents the longest transcript, and encodes the longer isoform (a). Both variants 1 and 3 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304827.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCTGC TCTGGGAGCC TGCAGGAGCC TGGCTTGCTC TGGGCCTGGC CCTGGCCCTG 
GGCCCCAGCG TGGCCGCAGC TGCCCCTCGG CAGGACTGCA CGGGCGTGGA GTGCCCGCCG 
CTGGAGAACT GCATTGAGGA GGCGCTGGAG CCGGGTGCCT GCTGTGCCAC GTGTGTGCAG 
CAGGGCTGCG CCTGCGAGGG CTACCAGTAC TATGACTGCC TACAGGGTGG CTTCGTGCGC 
GGCCGCGTGC CCGCCGGTCA GTCCTATTTT GTGGACTTCG GGAGCACTGA GTGCTCCTGC 
CCACCAGGCG GCGGCAAGAT CAGCTGCCAG TTCATGCTGT GCCCGGAGCT GCCGCCCAAC 
TGCATCGAGG CTGTAGTGGT GGCTGACAGC TGCCCACAGT GCGGCCAGGT GGGCTGCGTC 
CACGCGGGCC ACAAGTACGC CGCTGGCCAC ACTGTTCACC TGCCGCCCTG CCGGGCCTGC 
CACTGCCCTG ACGCCGGTGG AGAGCTCATC TGCTACCAGC TCCCCGGTTG CCACGGGAAC 
TTCTCAGATG CCGAGGAGGG TGACCCCGAG CGACACTACG AAGACCCCTA CAGCTATGAC 
CAGGAGGTGG CCGAGGTGGA AGCAGCAACA GCCCTGGGGG GTGAGGTCCA GGCGGGTGCA 
GTCCAGGCAG GCGCAGGGGG CCCCCCAGCT GCTCTGGGAG GTGGGAGTCA GCCACTGTCC 
ACCATCCAGG CACCCCCCTG GCCAGCTGTC CTCCCCAGGC CCACAGCGGC TGCTGCCCTG 
GGTCCCCCAG CCCCAGTGCA GGCCAAAGCT AGGAGAGTGA CCGAGGACAG TGAGGAGGAA 
GAAGAGGAGG AGGAGGAGAG AGAGGAAATG GCTGTCACTG AGCAGCTGGC AGCAGGTGGC 
CACAGGGGGC TGGATGGGCT GCCCACTACA GCCCCAGCTG GACCCAGTCT TCCTATCCAG 
GAGGAGAGGG CAGAAGCTGG GGCAAGGGCA GAAGCTGGGG CAAGGCCTGA AGAGAACCTC 
ATCCTGGATG CCCAAGCCAC GTCCCGCAGC ACTGGGCCGG AGGGCGTGAC GCATGCACCG 
AGCCTGGGCA AGGCTGCTCT CGTCCCAACT CAGGCCGTGC CTGGCTCTCC CAGGGACCCA 
GTCAAGCCCA GCCCCCACAA CATCCTGTCC ACATCACTGC CTGATGCAGC CTGGATCCCA 
CCCACCCGAG AAGTGCCCAG GAAGCCGCAA GTTCTGCCCC ATTCCCACGT GGAGGAGGAC 
ACAGACCCCA ACTCTGTCCA TTCTATCCCC AGAAGTAGCC CTGAAGGCTC CACCAAGGAC 
CTGATCGAGA CTTGCTGCGC AGCCGGACAG CAGTGGGCCA TTGACAATGA CGAGTGCCTG 
GAGATCCCTG AGAGTGGCAC TGAGGACAAC GTCTGCAGGA CAGCCCAGAG GCACTGCTGT 
GTCTCCTACT TGCAGGAGAA GAGCTGCATG GCCGGCGTCC TGGGAGCCAA GGAGGGTGAG 
ACCTGTGGGG CTGAGGACAA CGACAGCTGC GGCATCTCCC TGTACAAGCA ATGCTGTGAC 
TGCTGTGGCC TGGGCCTCCG CGTGCGGGCC GAGGGCCAGT CGTGTGAGTC CAATCCTAAC 
CTGGGCTATC CCTGCAATCA TGTCATGCTC TCCTGCTGTG AGGGTGAAGA GCCTCTCATA 
GTACCTGAGG TTCGCCGACC TCCAGAGCCC GCAGCTGCAC CACGGAGAGT TTCAGAGGCA 
GAGATGGCGG GCCGAGAGGC CCTGTCACTG GGCACAGAGG CCGAGCTGCC GAACAGCCTG 
CCGGGCGATG ACCAGGATGA GTGCCTTCTC CTCCCGGGAG AGCTGTGCCA GCACCTTTGC 
ATCAATACTG TGGGTTCTTA CCACTGTGCC TGCTTTCCTG GCTTCTCACT GCAGGACGAT 
GGCCGCACTT GCCGCCCAGA GGGTCACCCT CCACAGCCGG AAGCCCCACA GGAGCCTGCA 
CTGAAGTCAG AATTTTCCCA GGTGGCCTCT AACACCATCC CGCTGCCACT GCCGCAGCCC 
AATACCTGCA AAGACAATGG ACCCTGCAAG CAGGTGTGCA GCACTGTTGG GGGCTCAGCC 
ATATGCTCCT GTTTTCCCGG CTATGCCATC ATGGCGGATG GCGTGTCCTG TGAAGACCAA 
GACGAGTGCC TGATGGGTGC TCACGATTGT AGCCGGCGAC AGTTCTGTGT GAACACCCTG 
GGATCCTTCT ACTGTGTCAA CCACACAGTG CTCTGTGCCG ATGGCTATAT CCTCAATGCG 
CACAGGAAGT GCGTGGACAT CAACGAGTGT GTGACGGACC TGCACACGTG CAGCCGGGGC 
GAGCACTGTG TGAACACACT GGGCTCCTTC CACTGCTACA AGGCACTCAC CTGTGAGCCA 
GGCTATGCCC TCAAGGATGG CGAGTGCGAA GACGTGGATG AGTGTGCGAT GGGCACGCAC 
ACCTGCCAGC CGGGCTTCTT GTGCCAGAAC ACCAAGGGCT CCTTCTACTG CCAGGCCAGG 
CAGCGCTGCA TGGATGGCTT CCTGCAGGAT CCTGAAGGCA ACTGTGTGGA CATCAACGAG 
TGCACGTCAC TGTCCGAGCC ATGTCGGCCA GGCTTCAGCT GCATCAACAC GGTGGGCTCC 
TACACATGCC AGAGGAACCC GCTGATCTGC GCGCGCGGCT ACCACGCCAG CGATGATGGG 
ACCAAGTGTG TGGACGTGAA TGAGTGTGAG ACAGGTGTGC ACCGCTGCGG TGAGGGCCAA 
GTGTGCCACA ACCTCCCTGG CTCCTACCGC TGTGACTGCA AAGCCGGCTT TCAGCGGGAT 
GCCTTTGGCC GGGGCTGCAT CGACGTGAAT GAGTGCTGGG CCTCGCCAGG CCGCCTGTGC 
CAGCACACGT GTGAGAACAC ACTCGGCTCC TACCGCTGTT CCTGCGCCTC CGGGTTCCTG 
CTAGCAGCGG ACGGCAAGCG CTGTGAAGAC GTGAATGAGT GTGAGGCCCA GCGCTGCAGC 
CAGGAGTGTG CCAACATCTA TGGCTCCTAC CAGTGCTACT GCCGCCAGGG CTACCAGCTG 
GCTGAGGATG GGCACACCTG CACAGACATC GACGAGTGTG CTCAAGGCGC CGGCATCCTC 
TGCACCTTCC GCTGTCTCAA CGTGCCAGGG AGCTACCAGT GTGCATGCCC TGAGCAGGGC 
TACACCATGA CGGCCAACGG GAGGTCCTGC AAGGACGTGG ATGAGTGTGC ACTGGGTACC 
CACAACTGTT CCGAGGCTGA GACCTGCCAC AACATCCAGG GTAGCTTCCG CTGCCTGCGC 
TTCGAGTGTC CTCCCAACTA TGTCCAAGTC TCCAAAACGA AGTGCGAGCG CACCACGTGC 
CATGACTTCC TGGAGTGCCA GAACTCGCCA GCGCGCATCA CGCACTACCA GCTCAACTTC 
CAGACGGGCC TCCTGGTGCC TGCGCATATC TTCCGCATTG GCCCCGCGCC AGCCTTCACG 
GGGGACACCA TCGCCCTGAA CATCATCAAG GGCAATGAGG AGGGCTACTT TGGCACGCGC 
AGGCTCAATG CCTACACGGG TGTGGTCTAC CTGCAGCGGG CCGTGCTGGA GCCCCGGGAC 
TTTGCCCTGG ACGTGGAGAT GAAGCTCTGG AGGCAGGGCT CCGTCACCAC CTTCCTGGCC 
AAGATGCACA TCTTCTTCAC CACCTTTGCC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158507.1
CDS	383..4078
Translation	MVLLWEPAGAWLALGLALALGPSVAAAAPRQDCTGVECPPLENCIEEALEPGACCATCVQQGCACEGYQYYDCLQGGFVRGRVPAGQSYFVDFGSTECSCPPGGGKISCQFMLCPELPPNCIEAVVVADSCPQCGQVGCVHAGHKYAAGHTVHLPPCRACHCPDAGGELICYQLPGCHGNFSDAEEGDPERHYEDPYSYDQEVAEVEAATALGGEVQAGAVQAGAGGPPAALGGGSQPLSTIQAPPWPAVLPRPTAAAALGPPAPVQAKARRVTEDSEEEEEEEEEREEMAVTEQLAAGGHRGLDGLPTTAPAGPSLPIQEERAEAGARAEAGARPEENLILDAQATSRSTGPEGVTHAPSLGKAALVPTQAVPGSPRDPVKPSPHNILSTSLPDAAWIPPTREVPRKPQVLPHSHVEEDTDPNSVHSIPRSSPEGSTKDLIETCCAAGQQWAIDNDECLEIPESGTEDNVCRTAQRHCCVSYLQEKSCMAGVLGAKEGETCGAEDNDSCGISLYKQCCDCCGLGLRVRAEGQSCESNPNLGYPCNHVMLSCCEGEEPLIVPEVRRPPEPAAAPRRVSEAEMAGREALSLGTEAELPNSLPGDDQDECLLLPGELCQHLCINTVGSYHCACFPGFSLQDDGRTCRPEGHPPQPEAPQEPALKSEFSQVASNTIPLPLPQPNTCKDNGPCKQVCSTVGGSAICSCFPGYAIMADGVSCEDQDECLMGAHDCSRRQFCVNTLGSFYCVNHTVLCADGYILNAHRKCVDINECVTDLHTCSRGEHCVNTLGSFHCYKALTCEPGYALKDGECEDVDECAMGTHTCQPGFLCQNTKGSFYCQARQRCMDGFLQDPEGNCVDINECTSLSEPCRPGFSCINTVGSYTCQRNPLICARGYHASDDGTKCVDVNECETGVHRCGEGQVCHNLPGSYRCDCKAGFQRDAFGRGCIDVNECWASPGRLCQHTCENTLGSYRCSCASGFLLAADGKRCEDVNECEAQRCSQECANIYGSYQCYCRQGYQLAEDGHTCTDIDECAQGAGILCTFRCLNVPGSYQCACPEQGYTMTANGRSCKDVDECALGTHNCSEAETCHNIQGSFRCLRFECPPNYVQVSKTKCERTTCHDFLECQNSPARITHYQLNFQTGLLVPAHIFRIGPAPAFTGDTIALNIIKGNEEGYFGTRRLNAYTGVVYLQRAVLEPRDFALDVEMKLWRQGSVTTFLAKMHIFFTTFAL

Target ORF information:

RefSeq Version	NM_001165035.2
Organism	Homo sapiens(human)
Definition	Homo sapiens fibulin 2 (FBLN2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165035.2

ORF Insert Sequence:

ATGGTGCTGC TCTGGGAGCC TGCAGGAGCC TGGCTTGCTC TGGGCCTGGC CCTGGCCCTG 
GGCCCCAGCG TGGCCGCAGC TGCCCCTCGG CAGGACTGCA CGGGCGTGGA GTGCCCGCCG 
CTGGAGAACT GCATTGAGGA GGCGCTGGAG CCGGGTGCCT GCTGTGCCAC GTGTGTGCAG 
CAGGGCTGCG CCTGCGAGGG CTACCAGTAC TATGACTGCC TACAGGGTGG CTTCGTGCGC 
GGCCGCGTGC CCGCCGGTCA GTCCTATTTT GTGGACTTCG GGAGCACTGA GTGCTCCTGC 
CCACCAGGCG GCGGCAAGAT CAGCTGCCAG TTCATGCTGT GCCCGGAGCT GCCGCCCAAC 
TGCATCGAGG CTGTAGTGGT GGCTGACAGC TGCCCACAGT GCGGCCAGGT GGGCTGCGTC 
CACGCGGGCC ACAAGTACGC CGCTGGCCAC ACTGTTCACC TGCCGCCCTG CCGGGCCTGC 
CACTGCCCTG ACGCCGGTGG AGAGCTCATC TGCTACCAGC TCCCCGGTTG CCACGGGAAC 
TTCTCAGATG CCGAGGAGGG TGACCCCGAG CGACACTACG AAGACCCCTA CAGCTATGAC 
CAGGAGGTGG CCGAGGTGGA AGCAGCAACA GCCCTGGGGG GTGAGGTCCA GGCGGGTGCA 
GTCCAGGCAG GCGCAGGGGG CCCCCCAGCT GCTCTGGGAG GTGGGAGTCA GCCACTGTCC 
ACCATCCAGG CACCCCCCTG GCCAGCTGTC CTCCCCAGGC CCACAGCGGC TGCTGCCCTG 
GGTCCCCCAG CCCCAGTGCA GGCCAAAGCT AGGAGAGTGA CCGAGGACAG TGAGGAGGAA 
GAAGAGGAGG AGGAGGAGAG AGAGGAAATG GCTGTCACTG AGCAGCTGGC AGCAGGTGGC 
CACAGGGGGC TGGATGGGCT GCCCACTACA GCCCCAGCTG GACCCAGTCT TCCTATCCAG 
GAGGAGAGGG CAGAAGCTGG GGCAAGGGCA GAAGCTGGGG CAAGGCCTGA AGAGAACCTC 
ATCCTGGATG CCCAAGCCAC GTCCCGCAGC ACTGGGCCGG AGGGCGTGAC GCATGCACCG 
AGCCTGGGCA AGGCTGCTCT CGTCCCAACT CAGGCCGTGC CTGGCTCTCC CAGGGACCCA 
GTCAAGCCCA GCCCCCACAA CATCCTGTCC ACATCACTGC CTGATGCAGC CTGGATCCCA 
CCCACCCGAG AAGTGCCCAG GAAGCCGCAA GTTCTGCCCC ATTCCCACGT GGAGGAGGAC 
ACAGACCCCA ACTCTGTCCA TTCTATCCCC AGAAGTAGCC CTGAAGGCTC CACCAAGGAC 
CTGATCGAGA CTTGCTGCGC AGCCGGACAG CAGTGGGCCA TTGACAATGA CGAGTGCCTG 
GAGATCCCTG AGAGTGGCAC TGAGGACAAC GTCTGCAGGA CAGCCCAGAG GCACTGCTGT 
GTCTCCTACT TGCAGGAGAA GAGCTGCATG GCCGGCGTCC TGGGAGCCAA GGAGGGTGAG 
ACCTGTGGGG CTGAGGACAA CGACAGCTGC GGCATCTCCC TGTACAAGCA ATGCTGTGAC 
TGCTGTGGCC TGGGCCTCCG CGTGCGGGCC GAGGGCCAGT CGTGTGAGTC CAATCCTAAC 
CTGGGCTATC CCTGCAATCA TGTCATGCTC TCCTGCTGTG AGGGTGAAGA GCCTCTCATA 
GTACCTGAGG TTCGCCGACC TCCAGAGCCC GCAGCTGCAC CACGGAGAGT TTCAGAGGCA 
GAGATGGCGG GCCGAGAGGC CCTGTCACTG GGCACAGAGG CCGAGCTGCC GAACAGCCTG 
CCGGGCGATG ACCAGGATGA GTGCCTTCTC CTCCCGGGAG AGCTGTGCCA GCACCTTTGC 
ATCAATACTG TGGGTTCTTA CCACTGTGCC TGCTTTCCTG GCTTCTCACT GCAGGACGAT 
GGCCGCACTT GCCGCCCAGA GGGTCACCCT CCACAGCCGG AAGCCCCACA GGAGCCTGCA 
CTGAAGTCAG AATTTTCCCA GGTGGCCTCT AACACCATCC CGCTGCCACT GCCGCAGCCC 
AATACCTGCA AAGACAATGG ACCCTGCAAG CAGGTGTGCA GCACTGTTGG GGGCTCAGCC 
ATATGCTCCT GTTTTCCCGG CTATGCCATC ATGGCGGATG GCGTGTCCTG TGAAGACCAA 
GACGAGTGCC TGATGGGTGC TCACGATTGT AGCCGGCGAC AGTTCTGTGT GAACACCCTG 
GGATCCTTCT ACTGTGTCAA CCACACAGTG CTCTGTGCCG ATGGCTATAT CCTCAATGCG 
CACAGGAAGT GCGTGGACAT CAACGAGTGT GTGACGGACC TGCACACGTG CAGCCGGGGC 
GAGCACTGTG TGAACACACT GGGCTCCTTC CACTGCTACA AGGCACTCAC CTGTGAGCCA 
GGCTATGCCC TCAAGGATGG CGAGTGCGAA GACGTGGATG AGTGTGCGAT GGGCACGCAC 
ACCTGCCAGC CGGGCTTCTT GTGCCAGAAC ACCAAGGGCT CCTTCTACTG CCAGGCCAGG 
CAGCGCTGCA TGGATGGCTT CCTGCAGGAT CCTGAAGGCA ACTGTGTGGA CATCAACGAG 
TGCACGTCAC TGTCCGAGCC ATGTCGGCCA GGCTTCAGCT GCATCAACAC GGTGGGCTCC 
TACACATGCC AGAGGAACCC GCTGATCTGC GCGCGCGGCT ACCACGCCAG CGATGATGGG 
ACCAAGTGTG TGGACGTGAA TGAGTGTGAG ACAGGTGTGC ACCGCTGCGG TGAGGGCCAA 
GTGTGCCACA ACCTCCCTGG CTCCTACCGC TGTGACTGCA AAGCCGGCTT TCAGCGGGAT 
GCCTTTGGCC GGGGCTGCAT CGACGTGAAT GAGTGCTGGG CCTCGCCAGG CCGCCTGTGC 
CAGCACACGT GTGAGAACAC ACTCGGCTCC TACCGCTGTT CCTGCGCCTC CGGGTTCCTG 
CTAGCAGCGG ACGGCAAGCG CTGTGAAGAC GTGAATGAGT GTGAGGCCCA GCGCTGCAGC 
CAGGAGTGTG CCAACATCTA TGGCTCCTAC CAGTGCTACT GCCGCCAGGG CTACCAGCTG 
GCTGAGGATG GGCACACCTG CACAGACATC GACGAGTGTG CTCAAGGCGC CGGCATCCTC 
TGCACCTTCC GCTGTCTCAA CGTGCCAGGG AGCTACCAGT GTGCATGCCC TGAGCAGGGC 
TACACCATGA CGGCCAACGG GAGGTCCTGC AAGGACGTGG ATGAGTGTGC ACTGGGTACC 
CACAACTGTT CCGAGGCTGA GACCTGCCAC AACATCCAGG GTAGCTTCCG CTGCCTGCGC 
TTCGAGTGTC CTCCCAACTA TGTCCAAGTC TCCAAAACGA AGTGCGAGCG CACCACGTGC 
CATGACTTCC TGGAGTGCCA GAACTCGCCA GCGCGCATCA CGCACTACCA GCTCAACTTC 
CAGACGGGCC TCCTGGTGCC TGCGCATATC TTCCGCATTG GCCCCGCGCC AGCCTTCACG 
GGGGACACCA TCGCCCTGAA CATCATCAAG GGCAATGAGG AGGGCTACTT TGGCACGCGC 
AGGCTCAATG CCTACACGGG TGTGGTCTAC CTGCAGCGGG CCGTGCTGGA GCCCCGGGAC 
TTTGCCCTGG ACGTGGAGAT GAAGCTCTGG AGGCAGGGCT CCGTCACCAC CTTCCTGGCC 
AAGATGCACA TCTTCTTCAC CACCTTTGCC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FBLN2 ( NM_001998.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001989.2 Homo sapiens fibulin 2 (FBLN2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu29481
Clone ID Related Accession (Same CDS sequence)	NM_001998.3 , NM_001998.2
Accession Version	NM_001998.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	fibulin-2 isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB959783.1, BC051690.1 and AL050095.1. On May 31, 2019 this sequence version replaced NM_001998.2. Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR and lacks an alternate in-frame exon in the central coding region, compared to variant 3. The resulting isoform (b) lacks an internal segment, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1454417.1, SRR7346977.1874397.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCTGC TCTGGGAGCC TGCAGGAGCC TGGCTTGCTC TGGGCCTGGC CCTGGCCCTG 
GGCCCCAGCG TGGCCGCAGC TGCCCCTCGG CAGGACTGCA CGGGCGTGGA GTGCCCGCCG 
CTGGAGAACT GCATTGAGGA GGCGCTGGAG CCGGGTGCCT GCTGTGCCAC GTGTGTGCAG 
CAGGGCTGCG CCTGCGAGGG CTACCAGTAC TATGACTGCC TACAGGGTGG CTTCGTGCGC 
GGCCGCGTGC CCGCCGGTCA GTCCTATTTT GTGGACTTCG GGAGCACTGA GTGCTCCTGC 
CCACCAGGCG GCGGCAAGAT CAGCTGCCAG TTCATGCTGT GCCCGGAGCT GCCGCCCAAC 
TGCATCGAGG CTGTAGTGGT GGCTGACAGC TGCCCACAGT GCGGCCAGGT GGGCTGCGTC 
CACGCGGGCC ACAAGTACGC CGCTGGCCAC ACTGTTCACC TGCCGCCCTG CCGGGCCTGC 
CACTGCCCTG ACGCCGGTGG AGAGCTCATC TGCTACCAGC TCCCCGGTTG CCACGGGAAC 
TTCTCAGATG CCGAGGAGGG TGACCCCGAG CGACACTACG AAGACCCCTA CAGCTATGAC 
CAGGAGGTGG CCGAGGTGGA AGCAGCAACA GCCCTGGGGG GTGAGGTCCA GGCGGGTGCA 
GTCCAGGCAG GCGCAGGGGG CCCCCCAGCT GCTCTGGGAG GTGGGAGTCA GCCACTGTCC 
ACCATCCAGG CACCCCCCTG GCCAGCTGTC CTCCCCAGGC CCACAGCGGC TGCTGCCCTG 
GGTCCCCCAG CCCCAGTGCA GGCCAAAGCT AGGAGAGTGA CCGAGGACAG TGAGGAGGAA 
GAAGAGGAGG AGGAGGAGAG AGAGGAAATG GCTGTCACTG AGCAGCTGGC AGCAGGTGGC 
CACAGGGGGC TGGATGGGCT GCCCACTACA GCCCCAGCTG GACCCAGTCT TCCTATCCAG 
GAGGAGAGGG CAGAAGCTGG GGCAAGGGCA GAAGCTGGGG CAAGGCCTGA AGAGAACCTC 
ATCCTGGATG CCCAAGCCAC GTCCCGCAGC ACTGGGCCGG AGGGCGTGAC GCATGCACCG 
AGCCTGGGCA AGGCTGCTCT CGTCCCAACT CAGGCCGTGC CTGGCTCTCC CAGGGACCCA 
GTCAAGCCCA GCCCCCACAA CATCCTGTCC ACATCACTGC CTGATGCAGC CTGGATCCCA 
CCCACCCGAG AAGTGCCCAG GAAGCCGCAA GTTCTGCCCC ATTCCCACGT GGAGGAGGAC 
ACAGACCCCA ACTCTGTCCA TTCTATCCCC AGAAGTAGCC CTGAAGGCTC CACCAAGGAC 
CTGATCGAGA CTTGCTGCGC AGCCGGACAG CAGTGGGCCA TTGACAATGA CGAGTGCCTG 
GAGATCCCTG AGAGTGGCAC TGAGGACAAC GTCTGCAGGA CAGCCCAGAG GCACTGCTGT 
GTCTCCTACT TGCAGGAGAA GAGCTGCATG GCCGGCGTCC TGGGAGCCAA GGAGGGTGAG 
ACCTGTGGGG CTGAGGACAA CGACAGCTGC GGCATCTCCC TGTACAAGCA ATGCTGTGAC 
TGCTGTGGCC TGGGCCTCCG CGTGCGGGCC GAGGGCCAGT CGTGTGAGTC CAATCCTAAC 
CTGGGCTATC CCTGCAATCA TGTCATGCTC TCCTGCTGTG AGGGTGAAGA GCCTCTCATA 
GTACCTGAGG TTCGCCGACC TCCAGAGCCC GCAGCTGCAC CACGGAGAGT TTCAGAGGCA 
GAGATGGCGG GCCGAGAGGC CCTGTCACTG GGCACAGAGG CCGAGCTGCC GAACAGCCTG 
CCGGGCGATG ACCAGGATGA GTGCCTTCTC CTCCCGGGAG AGCTGTGCCA GCACCTTTGC 
ATCAATACTG TGGGTTCTTA CCACTGTGCC TGCTTTCCTG GCTTCTCACT GCAGGACGAT 
GGCCGCACTT GCCGCCCAGA GGGTCACCCT CCACAGCCGG AAGCCCCACA GGAGCCTGCA 
CTGAAGTCAG AATTTTCCCA GGTGGCCTCT AACACCATCC CGCTGCCACT GCCGCAGCCC 
AATACCTGCA AAGACAATGG ACCCTGCAAG CAGGTGTGCA GCACTGTTGG GGGCTCAGCC 
ATATGCTCCT GTTTTCCCGG CTATGCCATC ATGGCGGATG GCGTGTCCTG TGAAGACATC 
AACGAGTGTG TGACGGACCT GCACACGTGC AGCCGGGGCG AGCACTGTGT GAACACACTG 
GGCTCCTTCC ACTGCTACAA GGCACTCACC TGTGAGCCAG GCTATGCCCT CAAGGATGGC 
GAGTGCGAAG ACGTGGATGA GTGTGCGATG GGCACGCACA CCTGCCAGCC GGGCTTCTTG 
TGCCAGAACA CCAAGGGCTC CTTCTACTGC CAGGCCAGGC AGCGCTGCAT GGATGGCTTC 
CTGCAGGATC CTGAAGGCAA CTGTGTGGAC ATCAACGAGT GCACGTCACT GTCCGAGCCA 
TGTCGGCCAG GCTTCAGCTG CATCAACACG GTGGGCTCCT ACACATGCCA GAGGAACCCG 
CTGATCTGCG CGCGCGGCTA CCACGCCAGC GATGATGGGA CCAAGTGTGT GGACGTGAAT 
GAGTGTGAGA CAGGTGTGCA CCGCTGCGGT GAGGGCCAAG TGTGCCACAA CCTCCCTGGC 
TCCTACCGCT GTGACTGCAA AGCCGGCTTT CAGCGGGATG CCTTTGGCCG GGGCTGCATC 
GACGTGAATG AGTGCTGGGC CTCGCCAGGC CGCCTGTGCC AGCACACGTG TGAGAACACA 
CTCGGCTCCT ACCGCTGTTC CTGCGCCTCC GGGTTCCTGC TAGCAGCGGA CGGCAAGCGC 
TGTGAAGACG TGAATGAGTG TGAGGCCCAG CGCTGCAGCC AGGAGTGTGC CAACATCTAT 
GGCTCCTACC AGTGCTACTG CCGCCAGGGC TACCAGCTGG CTGAGGATGG GCACACCTGC 
ACAGACATCG ACGAGTGTGC TCAAGGCGCC GGCATCCTCT GCACCTTCCG CTGTCTCAAC 
GTGCCAGGGA GCTACCAGTG TGCATGCCCT GAGCAGGGCT ACACCATGAC GGCCAACGGG 
AGGTCCTGCA AGGACGTGGA TGAGTGTGCA CTGGGTACCC ACAACTGTTC CGAGGCTGAG 
ACCTGCCACA ACATCCAGGG TAGCTTCCGC TGCCTGCGCT TCGAGTGTCC TCCCAACTAT 
GTCCAAGTCT CCAAAACGAA GTGCGAGCGC ACCACGTGCC ATGACTTCCT GGAGTGCCAG 
AACTCGCCAG CGCGCATCAC GCACTACCAG CTCAACTTCC AGACGGGCCT CCTGGTGCCT 
GCGCATATCT TCCGCATTGG CCCCGCGCCA GCCTTCACGG GGGACACCAT CGCCCTGAAC 
ATCATCAAGG GCAATGAGGA GGGCTACTTT GGCACGCGCA GGCTCAATGC CTACACGGGT 
GTGGTCTACC TGCAGCGGGC CGTGCTGGAG CCCCGGGACT TTGCCCTGGA CGTGGAGATG 
AAGCTCTGGA GGCAGGGCTC CGTCACCACC TTCCTGGCCA AGATGCACAT CTTCTTCACC 
ACCTTTGCCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001989.2
CDS	126..3680
Translation	MVLLWEPAGAWLALGLALALGPSVAAAAPRQDCTGVECPPLENCIEEALEPGACCATCVQQGCACEGYQYYDCLQGGFVRGRVPAGQSYFVDFGSTECSCPPGGGKISCQFMLCPELPPNCIEAVVVADSCPQCGQVGCVHAGHKYAAGHTVHLPPCRACHCPDAGGELICYQLPGCHGNFSDAEEGDPERHYEDPYSYDQEVAEVEAATALGGEVQAGAVQAGAGGPPAALGGGSQPLSTIQAPPWPAVLPRPTAAAALGPPAPVQAKARRVTEDSEEEEEEEEEREEMAVTEQLAAGGHRGLDGLPTTAPAGPSLPIQEERAEAGARAEAGARPEENLILDAQATSRSTGPEGVTHAPSLGKAALVPTQAVPGSPRDPVKPSPHNILSTSLPDAAWIPPTREVPRKPQVLPHSHVEEDTDPNSVHSIPRSSPEGSTKDLIETCCAAGQQWAIDNDECLEIPESGTEDNVCRTAQRHCCVSYLQEKSCMAGVLGAKEGETCGAEDNDSCGISLYKQCCDCCGLGLRVRAEGQSCESNPNLGYPCNHVMLSCCEGEEPLIVPEVRRPPEPAAAPRRVSEAEMAGREALSLGTEAELPNSLPGDDQDECLLLPGELCQHLCINTVGSYHCACFPGFSLQDDGRTCRPEGHPPQPEAPQEPALKSEFSQVASNTIPLPLPQPNTCKDNGPCKQVCSTVGGSAICSCFPGYAIMADGVSCEDINECVTDLHTCSRGEHCVNTLGSFHCYKALTCEPGYALKDGECEDVDECAMGTHTCQPGFLCQNTKGSFYCQARQRCMDGFLQDPEGNCVDINECTSLSEPCRPGFSCINTVGSYTCQRNPLICARGYHASDDGTKCVDVNECETGVHRCGEGQVCHNLPGSYRCDCKAGFQRDAFGRGCIDVNECWASPGRLCQHTCENTLGSYRCSCASGFLLAADGKRCEDVNECEAQRCSQECANIYGSYQCYCRQGYQLAEDGHTCTDIDECAQGAGILCTFRCLNVPGSYQCACPEQGYTMTANGRSCKDVDECALGTHNCSEAETCHNIQGSFRCLRFECPPNYVQVSKTKCERTTCHDFLECQNSPARITHYQLNFQTGLLVPAHIFRIGPAPAFTGDTIALNIIKGNEEGYFGTRRLNAYTGVVYLQRAVLEPRDFALDVEMKLWRQGSVTTFLAKMHIFFTTFAL

Target ORF information:

RefSeq Version	NM_001998.3
Organism	Homo sapiens(human)
Definition	Homo sapiens fibulin 2 (FBLN2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001998.3

ORF Insert Sequence:

ATGGTGCTGC TCTGGGAGCC TGCAGGAGCC TGGCTTGCTC TGGGCCTGGC CCTGGCCCTG 
GGCCCCAGCG TGGCCGCAGC TGCCCCTCGG CAGGACTGCA CGGGCGTGGA GTGCCCGCCG 
CTGGAGAACT GCATTGAGGA GGCGCTGGAG CCGGGTGCCT GCTGTGCCAC GTGTGTGCAG 
CAGGGCTGCG CCTGCGAGGG CTACCAGTAC TATGACTGCC TACAGGGTGG CTTCGTGCGC 
GGCCGCGTGC CCGCCGGTCA GTCCTATTTT GTGGACTTCG GGAGCACTGA GTGCTCCTGC 
CCACCAGGCG GCGGCAAGAT CAGCTGCCAG TTCATGCTGT GCCCGGAGCT GCCGCCCAAC 
TGCATCGAGG CTGTAGTGGT GGCTGACAGC TGCCCACAGT GCGGCCAGGT GGGCTGCGTC 
CACGCGGGCC ACAAGTACGC CGCTGGCCAC ACTGTTCACC TGCCGCCCTG CCGGGCCTGC 
CACTGCCCTG ACGCCGGTGG AGAGCTCATC TGCTACCAGC TCCCCGGTTG CCACGGGAAC 
TTCTCAGATG CCGAGGAGGG TGACCCCGAG CGACACTACG AAGACCCCTA CAGCTATGAC 
CAGGAGGTGG CCGAGGTGGA AGCAGCAACA GCCCTGGGGG GTGAGGTCCA GGCGGGTGCA 
GTCCAGGCAG GCGCAGGGGG CCCCCCAGCT GCTCTGGGAG GTGGGAGTCA GCCACTGTCC 
ACCATCCAGG CACCCCCCTG GCCAGCTGTC CTCCCCAGGC CCACAGCGGC TGCTGCCCTG 
GGTCCCCCAG CCCCAGTGCA GGCCAAAGCT AGGAGAGTGA CCGAGGACAG TGAGGAGGAA 
GAAGAGGAGG AGGAGGAGAG AGAGGAAATG GCTGTCACTG AGCAGCTGGC AGCAGGTGGC 
CACAGGGGGC TGGATGGGCT GCCCACTACA GCCCCAGCTG GACCCAGTCT TCCTATCCAG 
GAGGAGAGGG CAGAAGCTGG GGCAAGGGCA GAAGCTGGGG CAAGGCCTGA AGAGAACCTC 
ATCCTGGATG CCCAAGCCAC GTCCCGCAGC ACTGGGCCGG AGGGCGTGAC GCATGCACCG 
AGCCTGGGCA AGGCTGCTCT CGTCCCAACT CAGGCCGTGC CTGGCTCTCC CAGGGACCCA 
GTCAAGCCCA GCCCCCACAA CATCCTGTCC ACATCACTGC CTGATGCAGC CTGGATCCCA 
CCCACCCGAG AAGTGCCCAG GAAGCCGCAA GTTCTGCCCC ATTCCCACGT GGAGGAGGAC 
ACAGACCCCA ACTCTGTCCA TTCTATCCCC AGAAGTAGCC CTGAAGGCTC CACCAAGGAC 
CTGATCGAGA CTTGCTGCGC AGCCGGACAG CAGTGGGCCA TTGACAATGA CGAGTGCCTG 
GAGATCCCTG AGAGTGGCAC TGAGGACAAC GTCTGCAGGA CAGCCCAGAG GCACTGCTGT 
GTCTCCTACT TGCAGGAGAA GAGCTGCATG GCCGGCGTCC TGGGAGCCAA GGAGGGTGAG 
ACCTGTGGGG CTGAGGACAA CGACAGCTGC GGCATCTCCC TGTACAAGCA ATGCTGTGAC 
TGCTGTGGCC TGGGCCTCCG CGTGCGGGCC GAGGGCCAGT CGTGTGAGTC CAATCCTAAC 
CTGGGCTATC CCTGCAATCA TGTCATGCTC TCCTGCTGTG AGGGTGAAGA GCCTCTCATA 
GTACCTGAGG TTCGCCGACC TCCAGAGCCC GCAGCTGCAC CACGGAGAGT TTCAGAGGCA 
GAGATGGCGG GCCGAGAGGC CCTGTCACTG GGCACAGAGG CCGAGCTGCC GAACAGCCTG 
CCGGGCGATG ACCAGGATGA GTGCCTTCTC CTCCCGGGAG AGCTGTGCCA GCACCTTTGC 
ATCAATACTG TGGGTTCTTA CCACTGTGCC TGCTTTCCTG GCTTCTCACT GCAGGACGAT 
GGCCGCACTT GCCGCCCAGA GGGTCACCCT CCACAGCCGG AAGCCCCACA GGAGCCTGCA 
CTGAAGTCAG AATTTTCCCA GGTGGCCTCT AACACCATCC CGCTGCCACT GCCGCAGCCC 
AATACCTGCA AAGACAATGG ACCCTGCAAG CAGGTGTGCA GCACTGTTGG GGGCTCAGCC 
ATATGCTCCT GTTTTCCCGG CTATGCCATC ATGGCGGATG GCGTGTCCTG TGAAGACATC 
AACGAGTGTG TGACGGACCT GCACACGTGC AGCCGGGGCG AGCACTGTGT GAACACACTG 
GGCTCCTTCC ACTGCTACAA GGCACTCACC TGTGAGCCAG GCTATGCCCT CAAGGATGGC 
GAGTGCGAAG ACGTGGATGA GTGTGCGATG GGCACGCACA CCTGCCAGCC GGGCTTCTTG 
TGCCAGAACA CCAAGGGCTC CTTCTACTGC CAGGCCAGGC AGCGCTGCAT GGATGGCTTC 
CTGCAGGATC CTGAAGGCAA CTGTGTGGAC ATCAACGAGT GCACGTCACT GTCCGAGCCA 
TGTCGGCCAG GCTTCAGCTG CATCAACACG GTGGGCTCCT ACACATGCCA GAGGAACCCG 
CTGATCTGCG CGCGCGGCTA CCACGCCAGC GATGATGGGA CCAAGTGTGT GGACGTGAAT 
GAGTGTGAGA CAGGTGTGCA CCGCTGCGGT GAGGGCCAAG TGTGCCACAA CCTCCCTGGC 
TCCTACCGCT GTGACTGCAA AGCCGGCTTT CAGCGGGATG CCTTTGGCCG GGGCTGCATC 
GACGTGAATG AGTGCTGGGC CTCGCCAGGC CGCCTGTGCC AGCACACGTG TGAGAACACA 
CTCGGCTCCT ACCGCTGTTC CTGCGCCTCC GGGTTCCTGC TAGCAGCGGA CGGCAAGCGC 
TGTGAAGACG TGAATGAGTG TGAGGCCCAG CGCTGCAGCC AGGAGTGTGC CAACATCTAT 
GGCTCCTACC AGTGCTACTG CCGCCAGGGC TACCAGCTGG CTGAGGATGG GCACACCTGC 
ACAGACATCG ACGAGTGTGC TCAAGGCGCC GGCATCCTCT GCACCTTCCG CTGTCTCAAC 
GTGCCAGGGA GCTACCAGTG TGCATGCCCT GAGCAGGGCT ACACCATGAC GGCCAACGGG 
AGGTCCTGCA AGGACGTGGA TGAGTGTGCA CTGGGTACCC ACAACTGTTC CGAGGCTGAG 
ACCTGCCACA ACATCCAGGG TAGCTTCCGC TGCCTGCGCT TCGAGTGTCC TCCCAACTAT 
GTCCAAGTCT CCAAAACGAA GTGCGAGCGC ACCACGTGCC ATGACTTCCT GGAGTGCCAG 
AACTCGCCAG CGCGCATCAC GCACTACCAG CTCAACTTCC AGACGGGCCT CCTGGTGCCT 
GCGCATATCT TCCGCATTGG CCCCGCGCCA GCCTTCACGG GGGACACCAT CGCCCTGAAC 
ATCATCAAGG GCAATGAGGA GGGCTACTTT GGCACGCGCA GGCTCAATGC CTACACGGGT 
GTGGTCTACC TGCAGCGGGC CGTGCTGGAG CCCCGGGACT TTGCCCTGGA CGTGGAGATG 
AAGCTCTGGA GGCAGGGCTC CGTCACCACC TTCCTGGCCA AGATGCACAT CTTCTTCACC 
ACCTTTGCCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FBLN2 ( NM_001998.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001989.2 Homo sapiens fibulin 2 (FBLN2), transcript variant 2, mRNA.

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CloneID	OHu29481
Clone ID Related Accession (Same CDS sequence)	NM_001998.3 , NM_001998.2
Accession Version	NM_001998.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	fibulin-2 isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB959783.1, BC051690.1, AL050095.1 and AY130459.1. On or before Aug 31, 2013 this sequence version replaced XM_005264950.1, NM_001998.1. Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR and lacks an alternate in-frame exon in the central coding region, compared to variant 3. The resulting isoform (b) lacks an internal segment, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X82494.1, SRR1803616.220385.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCTGC TCTGGGAGCC TGCAGGAGCC TGGCTTGCTC TGGGCCTGGC CCTGGCCCTG 
GGCCCCAGCG TGGCCGCAGC TGCCCCTCGG CAGGACTGCA CGGGCGTGGA GTGCCCGCCG 
CTGGAGAACT GCATTGAGGA GGCGCTGGAG CCGGGTGCCT GCTGTGCCAC GTGTGTGCAG 
CAGGGCTGCG CCTGCGAGGG CTACCAGTAC TATGACTGCC TACAGGGTGG CTTCGTGCGC 
GGCCGCGTGC CCGCCGGTCA GTCCTATTTT GTGGACTTCG GGAGCACTGA GTGCTCCTGC 
CCACCAGGCG GCGGCAAGAT CAGCTGCCAG TTCATGCTGT GCCCGGAGCT GCCGCCCAAC 
TGCATCGAGG CTGTAGTGGT GGCTGACAGC TGCCCACAGT GCGGCCAGGT GGGCTGCGTC 
CACGCGGGCC ACAAGTACGC CGCTGGCCAC ACTGTTCACC TGCCGCCCTG CCGGGCCTGC 
CACTGCCCTG ACGCCGGTGG AGAGCTCATC TGCTACCAGC TCCCCGGTTG CCACGGGAAC 
TTCTCAGATG CCGAGGAGGG TGACCCCGAG CGACACTACG AAGACCCCTA CAGCTATGAC 
CAGGAGGTGG CCGAGGTGGA AGCAGCAACA GCCCTGGGGG GTGAGGTCCA GGCGGGTGCA 
GTCCAGGCAG GCGCAGGGGG CCCCCCAGCT GCTCTGGGAG GTGGGAGTCA GCCACTGTCC 
ACCATCCAGG CACCCCCCTG GCCAGCTGTC CTCCCCAGGC CCACAGCGGC TGCTGCCCTG 
GGTCCCCCAG CCCCAGTGCA GGCCAAAGCT AGGAGAGTGA CCGAGGACAG TGAGGAGGAA 
GAAGAGGAGG AGGAGGAGAG AGAGGAAATG GCTGTCACTG AGCAGCTGGC AGCAGGTGGC 
CACAGGGGGC TGGATGGGCT GCCCACTACA GCCCCAGCTG GACCCAGTCT TCCTATCCAG 
GAGGAGAGGG CAGAAGCTGG GGCAAGGGCA GAAGCTGGGG CAAGGCCTGA AGAGAACCTC 
ATCCTGGATG CCCAAGCCAC GTCCCGCAGC ACTGGGCCGG AGGGCGTGAC GCATGCACCG 
AGCCTGGGCA AGGCTGCTCT CGTCCCAACT CAGGCCGTGC CTGGCTCTCC CAGGGACCCA 
GTCAAGCCCA GCCCCCACAA CATCCTGTCC ACATCACTGC CTGATGCAGC CTGGATCCCA 
CCCACCCGAG AAGTGCCCAG GAAGCCGCAA GTTCTGCCCC ATTCCCACGT GGAGGAGGAC 
ACAGACCCCA ACTCTGTCCA TTCTATCCCC AGAAGTAGCC CTGAAGGCTC CACCAAGGAC 
CTGATCGAGA CTTGCTGCGC AGCCGGACAG CAGTGGGCCA TTGACAATGA CGAGTGCCTG 
GAGATCCCTG AGAGTGGCAC TGAGGACAAC GTCTGCAGGA CAGCCCAGAG GCACTGCTGT 
GTCTCCTACT TGCAGGAGAA GAGCTGCATG GCCGGCGTCC TGGGAGCCAA GGAGGGTGAG 
ACCTGTGGGG CTGAGGACAA CGACAGCTGC GGCATCTCCC TGTACAAGCA ATGCTGTGAC 
TGCTGTGGCC TGGGCCTCCG CGTGCGGGCC GAGGGCCAGT CGTGTGAGTC CAATCCTAAC 
CTGGGCTATC CCTGCAATCA TGTCATGCTC TCCTGCTGTG AGGGTGAAGA GCCTCTCATA 
GTACCTGAGG TTCGCCGACC TCCAGAGCCC GCAGCTGCAC CACGGAGAGT TTCAGAGGCA 
GAGATGGCGG GCCGAGAGGC CCTGTCACTG GGCACAGAGG CCGAGCTGCC GAACAGCCTG 
CCGGGCGATG ACCAGGATGA GTGCCTTCTC CTCCCGGGAG AGCTGTGCCA GCACCTTTGC 
ATCAATACTG TGGGTTCTTA CCACTGTGCC TGCTTTCCTG GCTTCTCACT GCAGGACGAT 
GGCCGCACTT GCCGCCCAGA GGGTCACCCT CCACAGCCGG AAGCCCCACA GGAGCCTGCA 
CTGAAGTCAG AATTTTCCCA GGTGGCCTCT AACACCATCC CGCTGCCACT GCCGCAGCCC 
AATACCTGCA AAGACAATGG ACCCTGCAAG CAGGTGTGCA GCACTGTTGG GGGCTCAGCC 
ATATGCTCCT GTTTTCCCGG CTATGCCATC ATGGCGGATG GCGTGTCCTG TGAAGACATC 
AACGAGTGTG TGACGGACCT GCACACGTGC AGCCGGGGCG AGCACTGTGT GAACACACTG 
GGCTCCTTCC ACTGCTACAA GGCACTCACC TGTGAGCCAG GCTATGCCCT CAAGGATGGC 
GAGTGCGAAG ACGTGGATGA GTGTGCGATG GGCACGCACA CCTGCCAGCC GGGCTTCTTG 
TGCCAGAACA CCAAGGGCTC CTTCTACTGC CAGGCCAGGC AGCGCTGCAT GGATGGCTTC 
CTGCAGGATC CTGAAGGCAA CTGTGTGGAC ATCAACGAGT GCACGTCACT GTCCGAGCCA 
TGTCGGCCAG GCTTCAGCTG CATCAACACG GTGGGCTCCT ACACATGCCA GAGGAACCCG 
CTGATCTGCG CGCGCGGCTA CCACGCCAGC GATGATGGGA CCAAGTGTGT GGACGTGAAT 
GAGTGTGAGA CAGGTGTGCA CCGCTGCGGT GAGGGCCAAG TGTGCCACAA CCTCCCTGGC 
TCCTACCGCT GTGACTGCAA AGCCGGCTTT CAGCGGGATG CCTTTGGCCG GGGCTGCATC 
GACGTGAATG AGTGCTGGGC CTCGCCAGGC CGCCTGTGCC AGCACACGTG TGAGAACACA 
CTCGGCTCCT ACCGCTGTTC CTGCGCCTCC GGGTTCCTGC TAGCAGCGGA CGGCAAGCGC 
TGTGAAGACG TGAATGAGTG TGAGGCCCAG CGCTGCAGCC AGGAGTGTGC CAACATCTAT 
GGCTCCTACC AGTGCTACTG CCGCCAGGGC TACCAGCTGG CTGAGGATGG GCACACCTGC 
ACAGACATCG ACGAGTGTGC TCAAGGCGCC GGCATCCTCT GCACCTTCCG CTGTCTCAAC 
GTGCCAGGGA GCTACCAGTG TGCATGCCCT GAGCAGGGCT ACACCATGAC GGCCAACGGG 
AGGTCCTGCA AGGACGTGGA TGAGTGTGCA CTGGGTACCC ACAACTGTTC CGAGGCTGAG 
ACCTGCCACA ACATCCAGGG TAGCTTCCGC TGCCTGCGCT TCGAGTGTCC TCCCAACTAT 
GTCCAAGTCT CCAAAACGAA GTGCGAGCGC ACCACGTGCC ATGACTTCCT GGAGTGCCAG 
AACTCGCCAG CGCGCATCAC GCACTACCAG CTCAACTTCC AGACGGGCCT CCTGGTGCCT 
GCGCATATCT TCCGCATTGG CCCCGCGCCA GCCTTCACGG GGGACACCAT CGCCCTGAAC 
ATCATCAAGG GCAATGAGGA GGGCTACTTT GGCACGCGCA GGCTCAATGC CTACACGGGT 
GTGGTCTACC TGCAGCGGGC CGTGCTGGAG CCCCGGGACT TTGCCCTGGA CGTGGAGATG 
AAGCTCTGGA GGCAGGGCTC CGTCACCACC TTCCTGGCCA AGATGCACAT CTTCTTCACC 
ACCTTTGCCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001989.2
CDS	126..3680
Translation	MVLLWEPAGAWLALGLALALGPSVAAAAPRQDCTGVECPPLENCIEEALEPGACCATCVQQGCACEGYQYYDCLQGGFVRGRVPAGQSYFVDFGSTECSCPPGGGKISCQFMLCPELPPNCIEAVVVADSCPQCGQVGCVHAGHKYAAGHTVHLPPCRACHCPDAGGELICYQLPGCHGNFSDAEEGDPERHYEDPYSYDQEVAEVEAATALGGEVQAGAVQAGAGGPPAALGGGSQPLSTIQAPPWPAVLPRPTAAAALGPPAPVQAKARRVTEDSEEEEEEEEEREEMAVTEQLAAGGHRGLDGLPTTAPAGPSLPIQEERAEAGARAEAGARPEENLILDAQATSRSTGPEGVTHAPSLGKAALVPTQAVPGSPRDPVKPSPHNILSTSLPDAAWIPPTREVPRKPQVLPHSHVEEDTDPNSVHSIPRSSPEGSTKDLIETCCAAGQQWAIDNDECLEIPESGTEDNVCRTAQRHCCVSYLQEKSCMAGVLGAKEGETCGAEDNDSCGISLYKQCCDCCGLGLRVRAEGQSCESNPNLGYPCNHVMLSCCEGEEPLIVPEVRRPPEPAAAPRRVSEAEMAGREALSLGTEAELPNSLPGDDQDECLLLPGELCQHLCINTVGSYHCACFPGFSLQDDGRTCRPEGHPPQPEAPQEPALKSEFSQVASNTIPLPLPQPNTCKDNGPCKQVCSTVGGSAICSCFPGYAIMADGVSCEDINECVTDLHTCSRGEHCVNTLGSFHCYKALTCEPGYALKDGECEDVDECAMGTHTCQPGFLCQNTKGSFYCQARQRCMDGFLQDPEGNCVDINECTSLSEPCRPGFSCINTVGSYTCQRNPLICARGYHASDDGTKCVDVNECETGVHRCGEGQVCHNLPGSYRCDCKAGFQRDAFGRGCIDVNECWASPGRLCQHTCENTLGSYRCSCASGFLLAADGKRCEDVNECEAQRCSQECANIYGSYQCYCRQGYQLAEDGHTCTDIDECAQGAGILCTFRCLNVPGSYQCACPEQGYTMTANGRSCKDVDECALGTHNCSEAETCHNIQGSFRCLRFECPPNYVQVSKTKCERTTCHDFLECQNSPARITHYQLNFQTGLLVPAHIFRIGPAPAFTGDTIALNIIKGNEEGYFGTRRLNAYTGVVYLQRAVLEPRDFALDVEMKLWRQGSVTTFLAKMHIFFTTFAL

Target ORF information:

RefSeq Version	NM_001998.2
Organism	Homo sapiens(human)
Definition	Homo sapiens fibulin 2 (FBLN2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001998.2

ORF Insert Sequence:

ATGGTGCTGC TCTGGGAGCC TGCAGGAGCC TGGCTTGCTC TGGGCCTGGC CCTGGCCCTG 
GGCCCCAGCG TGGCCGCAGC TGCCCCTCGG CAGGACTGCA CGGGCGTGGA GTGCCCGCCG 
CTGGAGAACT GCATTGAGGA GGCGCTGGAG CCGGGTGCCT GCTGTGCCAC GTGTGTGCAG 
CAGGGCTGCG CCTGCGAGGG CTACCAGTAC TATGACTGCC TACAGGGTGG CTTCGTGCGC 
GGCCGCGTGC CCGCCGGTCA GTCCTATTTT GTGGACTTCG GGAGCACTGA GTGCTCCTGC 
CCACCAGGCG GCGGCAAGAT CAGCTGCCAG TTCATGCTGT GCCCGGAGCT GCCGCCCAAC 
TGCATCGAGG CTGTAGTGGT GGCTGACAGC TGCCCACAGT GCGGCCAGGT GGGCTGCGTC 
CACGCGGGCC ACAAGTACGC CGCTGGCCAC ACTGTTCACC TGCCGCCCTG CCGGGCCTGC 
CACTGCCCTG ACGCCGGTGG AGAGCTCATC TGCTACCAGC TCCCCGGTTG CCACGGGAAC 
TTCTCAGATG CCGAGGAGGG TGACCCCGAG CGACACTACG AAGACCCCTA CAGCTATGAC 
CAGGAGGTGG CCGAGGTGGA AGCAGCAACA GCCCTGGGGG GTGAGGTCCA GGCGGGTGCA 
GTCCAGGCAG GCGCAGGGGG CCCCCCAGCT GCTCTGGGAG GTGGGAGTCA GCCACTGTCC 
ACCATCCAGG CACCCCCCTG GCCAGCTGTC CTCCCCAGGC CCACAGCGGC TGCTGCCCTG 
GGTCCCCCAG CCCCAGTGCA GGCCAAAGCT AGGAGAGTGA CCGAGGACAG TGAGGAGGAA 
GAAGAGGAGG AGGAGGAGAG AGAGGAAATG GCTGTCACTG AGCAGCTGGC AGCAGGTGGC 
CACAGGGGGC TGGATGGGCT GCCCACTACA GCCCCAGCTG GACCCAGTCT TCCTATCCAG 
GAGGAGAGGG CAGAAGCTGG GGCAAGGGCA GAAGCTGGGG CAAGGCCTGA AGAGAACCTC 
ATCCTGGATG CCCAAGCCAC GTCCCGCAGC ACTGGGCCGG AGGGCGTGAC GCATGCACCG 
AGCCTGGGCA AGGCTGCTCT CGTCCCAACT CAGGCCGTGC CTGGCTCTCC CAGGGACCCA 
GTCAAGCCCA GCCCCCACAA CATCCTGTCC ACATCACTGC CTGATGCAGC CTGGATCCCA 
CCCACCCGAG AAGTGCCCAG GAAGCCGCAA GTTCTGCCCC ATTCCCACGT GGAGGAGGAC 
ACAGACCCCA ACTCTGTCCA TTCTATCCCC AGAAGTAGCC CTGAAGGCTC CACCAAGGAC 
CTGATCGAGA CTTGCTGCGC AGCCGGACAG CAGTGGGCCA TTGACAATGA CGAGTGCCTG 
GAGATCCCTG AGAGTGGCAC TGAGGACAAC GTCTGCAGGA CAGCCCAGAG GCACTGCTGT 
GTCTCCTACT TGCAGGAGAA GAGCTGCATG GCCGGCGTCC TGGGAGCCAA GGAGGGTGAG 
ACCTGTGGGG CTGAGGACAA CGACAGCTGC GGCATCTCCC TGTACAAGCA ATGCTGTGAC 
TGCTGTGGCC TGGGCCTCCG CGTGCGGGCC GAGGGCCAGT CGTGTGAGTC CAATCCTAAC 
CTGGGCTATC CCTGCAATCA TGTCATGCTC TCCTGCTGTG AGGGTGAAGA GCCTCTCATA 
GTACCTGAGG TTCGCCGACC TCCAGAGCCC GCAGCTGCAC CACGGAGAGT TTCAGAGGCA 
GAGATGGCGG GCCGAGAGGC CCTGTCACTG GGCACAGAGG CCGAGCTGCC GAACAGCCTG 
CCGGGCGATG ACCAGGATGA GTGCCTTCTC CTCCCGGGAG AGCTGTGCCA GCACCTTTGC 
ATCAATACTG TGGGTTCTTA CCACTGTGCC TGCTTTCCTG GCTTCTCACT GCAGGACGAT 
GGCCGCACTT GCCGCCCAGA GGGTCACCCT CCACAGCCGG AAGCCCCACA GGAGCCTGCA 
CTGAAGTCAG AATTTTCCCA GGTGGCCTCT AACACCATCC CGCTGCCACT GCCGCAGCCC 
AATACCTGCA AAGACAATGG ACCCTGCAAG CAGGTGTGCA GCACTGTTGG GGGCTCAGCC 
ATATGCTCCT GTTTTCCCGG CTATGCCATC ATGGCGGATG GCGTGTCCTG TGAAGACATC 
AACGAGTGTG TGACGGACCT GCACACGTGC AGCCGGGGCG AGCACTGTGT GAACACACTG 
GGCTCCTTCC ACTGCTACAA GGCACTCACC TGTGAGCCAG GCTATGCCCT CAAGGATGGC 
GAGTGCGAAG ACGTGGATGA GTGTGCGATG GGCACGCACA CCTGCCAGCC GGGCTTCTTG 
TGCCAGAACA CCAAGGGCTC CTTCTACTGC CAGGCCAGGC AGCGCTGCAT GGATGGCTTC 
CTGCAGGATC CTGAAGGCAA CTGTGTGGAC ATCAACGAGT GCACGTCACT GTCCGAGCCA 
TGTCGGCCAG GCTTCAGCTG CATCAACACG GTGGGCTCCT ACACATGCCA GAGGAACCCG 
CTGATCTGCG CGCGCGGCTA CCACGCCAGC GATGATGGGA CCAAGTGTGT GGACGTGAAT 
GAGTGTGAGA CAGGTGTGCA CCGCTGCGGT GAGGGCCAAG TGTGCCACAA CCTCCCTGGC 
TCCTACCGCT GTGACTGCAA AGCCGGCTTT CAGCGGGATG CCTTTGGCCG GGGCTGCATC 
GACGTGAATG AGTGCTGGGC CTCGCCAGGC CGCCTGTGCC AGCACACGTG TGAGAACACA 
CTCGGCTCCT ACCGCTGTTC CTGCGCCTCC GGGTTCCTGC TAGCAGCGGA CGGCAAGCGC 
TGTGAAGACG TGAATGAGTG TGAGGCCCAG CGCTGCAGCC AGGAGTGTGC CAACATCTAT 
GGCTCCTACC AGTGCTACTG CCGCCAGGGC TACCAGCTGG CTGAGGATGG GCACACCTGC 
ACAGACATCG ACGAGTGTGC TCAAGGCGCC GGCATCCTCT GCACCTTCCG CTGTCTCAAC 
GTGCCAGGGA GCTACCAGTG TGCATGCCCT GAGCAGGGCT ACACCATGAC GGCCAACGGG 
AGGTCCTGCA AGGACGTGGA TGAGTGTGCA CTGGGTACCC ACAACTGTTC CGAGGCTGAG 
ACCTGCCACA ACATCCAGGG TAGCTTCCGC TGCCTGCGCT TCGAGTGTCC TCCCAACTAT 
GTCCAAGTCT CCAAAACGAA GTGCGAGCGC ACCACGTGCC ATGACTTCCT GGAGTGCCAG 
AACTCGCCAG CGCGCATCAC GCACTACCAG CTCAACTTCC AGACGGGCCT CCTGGTGCCT 
GCGCATATCT TCCGCATTGG CCCCGCGCCA GCCTTCACGG GGGACACCAT CGCCCTGAAC 
ATCATCAAGG GCAATGAGGA GGGCTACTTT GGCACGCGCA GGCTCAATGC CTACACGGGT 
GTGGTCTACC TGCAGCGGGC CGTGCTGGAG CCCCGGGACT TTGCCCTGGA CGTGGAGATG 
AAGCTCTGGA GGCAGGGCTC CGTCACCACC TTCCTGGCCA AGATGCACAT CTTCTTCACC 
ACCTTTGCCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FBLN2 ( XM_006713026.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713089.1 Homo sapiens fibulin 2 (FBLN2), transcript variant X1, mRNA.

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CloneID	OHu37044
Clone ID Related Accession (Same CDS sequence)	XM_006713026.2
Accession Version	XM_006713026.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	fibulin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006713026.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGC TCTGGGAGCC TGCAGGAGCC TGGCTTGCTC TGGGCCTGGC CCTGGCCCTG 
GGCCCCAGCG TGGCCGCAGC TGCCCCTCGG CAGGACTGCA CGGGCGTGGA GTGCCCGCCG 
CTGGAGAACT GCATTGAGGA GGCGCTGGAG CCGGGTGCCT GCTGTGCCAC GTGTGTGCAG 
CAGGGCTGCG CCTGCGAGGG CTACCAGTAC TATGACTGCC TACAGGGTGG CTTCGTGCGC 
GGCCGCGTGC CCGCCGGTCA GTCCTATTTT GTGGACTTCG GGAGCACTGA GTGCTCCTGC 
CCACCAGGCG GCGGCAAGAT CAGCTGCCAG TTCATGCTGT GCCCGGAGCT GCCGCCCAAC 
TGCATCGAGG CTGTAGTGGT GGCTGACAGC TGCCCACAGT GCGGCCAGGT GGGCTGCGTC 
CACGCGGGCC ACAAGTACGC CGCTGGCCAC ACTGTTCACC TGCCGCCCTG CCGGGCCTGC 
CACTGCCCTG ACGCCGGTGG AGAGCTCATC TGCTACCAGC TCCCCGGTTG CCACGGGAAC 
TTCTCAGATG CCGAGGAGGG TGACCCCGAG CGACACTACG AAGACCCCTA CAGCTATGAC 
CAGGAGGTGG CCGAGGTGGA AGCAGCAACA GCCCTGGGGG GTGAGGTCCA GGCGGGTGCA 
GTCCAGGCAG GCGCAGGGGG CCCCCCAGCT GCTCTGGGAG GTGGGAGTCA GCCACTGTCC 
ACCATCCAGG CACCCCCCTG GCCAGCTGTC CTCCCCAGGC CCACAGCGGC TGCTGCCCTG 
GGTCCCCCAG CCCCAGTGCA GGCCAAAGCT AGGAGAGTGA CCGAGGACAG TGAGGAGGAA 
GAAGAGGAGG AGGAGGAGAG AGAGGAAATG GCTGTCACTG AGCAGCTGGC AGCAGGTGGC 
CACAGGGGGC TGGATGGGCT GCCCACTACA GCCCCAGCTG GACCCAGTCT TCCTATCCAG 
GAGGAGAGGG CAGAAGCTGG GGCAAGGGCA GAAGCTGGGG CAAGGCCTGA AGAGAACCTC 
ATCCTGGATG CCCAAGCCAC GTCCCGCAGC ACTGGGCCGG AGGGCGTGAC GCATGCACCG 
AGCCTGGGCA AGGCTGCTCT CGTCCCAACT CAGGCCGTGC CTGGCTCTCC CAGGGACCCA 
GTCAAGCCCA GCCCCCACAA CATCCTGTCC ACATCACTGC CTGATGCAGC CTGGATCCCA 
CCCACCCGAG AAGTGCCCAG GAAGCCGCAA GTTCTGCCCC ATTCCCACGT GGAGGAGGAC 
ACAGACCCCA ACTCTGTCCA TTCTATCCCC AGAAGTAGCC CTGAAGGCTC CACCAAGGAC 
CTGATCGAGA CTTGCTGCGC AGCCGGACAG CAGTGGGCCA TTGACAATGA CGAGTGCCTG 
GAGATCCCTG AGAGTGGCAC TGAGGACAAC GTCTGCAGGA CAGCCCAGAG GCACTGCTGT 
GTCTCCTACT TGCAGGAGAA GAGCTGCATG GCCGGCGTCC TGGGAGCCAA GGAGGGTGAG 
ACCTGTGGGG CTGAGGACAA CGACAGCTGC GGCATCTCCC TGTACAAGCA ATGCTGTGAC 
TGCTGTGGCC TGGGCCTCCG CGTGCGGGCC GAGGGCCAGT CGTGTGAGTC CAATCCTAAC 
CTGGGCTATC CCTGCAATCA TGTCATGCTC TCCTGCTGTG AGGGTGAAGA GCCTCTCATA 
GTACCTGAGG TTCGCCGACC TCCAGAGCCC GCAGCTGCAC CACGGAGAGT TTCAGAGGCA 
GAGATGGCGG GCCGAGAGGC CCTGTCACTG GGCACAGAGG CCGAGCTGCC GAACAGCCTG 
CCGGGCGATG ACCAGGATGA GTGCCTTCTC CTCCCGGGAG AGCTGTGCCA GCACCTTTGC 
ATCAATACTG TGGGTTCTTA CCACTGTGCC TGCTTTCCTG GCTTCTCACT GCAGGACGAT 
GGCCGCACTT GCCGCCCAGA GGGTCACCCT CCACAGCCGG AAGCCCCACA GGAGCCTGCA 
CTGAAGTCAG AATTTTCCCA GGTGGCCTCT AACACCATCC CGCTGCCACT GCCGCAGCCC 
AATACCTGCA AAGACAATGG ACCCTGCAAG CAGGTGTGCA GCACTGTTGG GGGCTCAGCC 
ATATGCTCCT GTTTTCCCGG CTATGCCATC ATGGCGGATG GCGTGTCCTG TGAAGACCAA 
GACGAGTGCC TGATGGGTGC TCACGATTGT AGCCGGCGAC AGTTCTGTGT GAACACCCTG 
GGATCCTTCT ACTGTGTCAA CCACACAGTG CTCTGTGCCG ATGGCTATAT CCTCAATGCG 
CACAGGAAGT GCGTGGACAT CAACGAGTGT GTGACGGACC TGCACACGTG CAGCCGGGGC 
GAGCACTGTG TGAACACACT GGGCTCCTTC CACTGCTACA AGGCACTCAC CTGTGAGCCA 
GGCTATGCCC TCAAGGATGG CGAGTGCGAA GACGTGGATG AGTGTGCGAT GGGCACGCAC 
ACCTGCCAGC CGGGCTTCTT GTGCCAGAAC ACCAAGGGCT CCTTCTACTG CCAGGCCAGG 
CAGCGCTGCA TGGATGGCTT CCTGCAGGAT CCTGAAGGCA ACTGTGTGGA CATCAACGAG 
TGCACGTCAC TGTCCGAGCC ATGTCGGCCA GGCTTCAGCT GCATCAACAC GGTGGGCTCC 
TACACATGCC AGAGGAACCC GCTGATCTGC GCGCGCGGCT ACCACGCCAG CGATGATGGG 
ACCAAGTGTG TGGACGTGAA TGAGTGTGAG ACAGGTGTGC ACCGCTGCGG TGAGGGCCAA 
GTGTGCCACA ACCTCCCTGG CTCCTACCGC TGTGACTGCA AAGCCGGCTT TCAGCGGGAT 
GCCTTTGGCC GGGGCTGCAT CGACGTGAAT GAGTGCTGGG CCTCGCCAGG CCGCCTGTGC 
CAGCACACGT GTGAGAACAC ACTCGGCTCC TACCGCTGTT CCTGCGCCTC CGGGTTCCTG 
CTAGCAGCGG ACGGCAAGCG CTGTGAAGAC ATCGACGAGT GTGCTCAAGG CGCCGGCATC 
CTCTGCACCT TCCGCTGTCT CAACGTGCCA GGGAGCTACC AGTGTGCATG CCCTGAGCAG 
GGCTACACCA TGACGGCCAA CGGGAGGTCC TGCAAGGACG TGGATGAGTG TGCACTGGGT 
ACCCACAACT GTTCCGAGGC TGAGACCTGC CACAACATCC AGGGTAGCTT CCGCTGCCTG 
CGCTTCGAGT GTCCTCCCAA CTATGTCCAA GTCTCCAAAA CGAAGTGCGA GCGCACCACG 
TGCCATGACT TCCTGGAGTG CCAGAACTCG CCAGCGCGCA TCACGCACTA CCAGCTCAAC 
TTCCAGACGG GCCTCCTGGT GCCTGCGCAT ATCTTCCGCA TTGGCCCCGC GCCAGCCTTC 
ACGGGGGACA CCATCGCCCT GAACATCATC AAGGGCAATG AGGAGGGCTA CTTTGGCACG 
CGCAGGCTCA ATGCCTACAC GGGTGTGGTC TACCTGCAGC GGGCCGTGCT GGAGCCCCGG 
GACTTTGCCC TGGACGTGGA GATGAAGCTC TGGAGGCAGG GCTCCGTCAC CACCTTCCTG 
GCCAAGATGC ACATCTTCTT CACCACCTTT GCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713089.1
CDS	35..3613
Translation	MVLLWEPAGAWLALGLALALGPSVAAAAPRQDCTGVECPPLENCIEEALEPGACCATCVQQGCACEGYQYYDCLQGGFVRGRVPAGQSYFVDFGSTECSCPPGGGKISCQFMLCPELPPNCIEAVVVADSCPQCGQVGCVHAGHKYAAGHTVHLPPCRACHCPDAGGELICYQLPGCHGNFSDAEEGDPERHYEDPYSYDQEVAEVEAATALGGEVQAGAVQAGAGGPPAALGGGSQPLSTIQAPPWPAVLPRPTAAAALGPPAPVQAKARRVTEDSEEEEEEEEEREEMAVTEQLAAGGHRGLDGLPTTAPAGPSLPIQEERAEAGARAEAGARPEENLILDAQATSRSTGPEGVTHAPSLGKAALVPTQAVPGSPRDPVKPSPHNILSTSLPDAAWIPPTREVPRKPQVLPHSHVEEDTDPNSVHSIPRSSPEGSTKDLIETCCAAGQQWAIDNDECLEIPESGTEDNVCRTAQRHCCVSYLQEKSCMAGVLGAKEGETCGAEDNDSCGISLYKQCCDCCGLGLRVRAEGQSCESNPNLGYPCNHVMLSCCEGEEPLIVPEVRRPPEPAAAPRRVSEAEMAGREALSLGTEAELPNSLPGDDQDECLLLPGELCQHLCINTVGSYHCACFPGFSLQDDGRTCRPEGHPPQPEAPQEPALKSEFSQVASNTIPLPLPQPNTCKDNGPCKQVCSTVGGSAICSCFPGYAIMADGVSCEDQDECLMGAHDCSRRQFCVNTLGSFYCVNHTVLCADGYILNAHRKCVDINECVTDLHTCSRGEHCVNTLGSFHCYKALTCEPGYALKDGECEDVDECAMGTHTCQPGFLCQNTKGSFYCQARQRCMDGFLQDPEGNCVDINECTSLSEPCRPGFSCINTVGSYTCQRNPLICARGYHASDDGTKCVDVNECETGVHRCGEGQVCHNLPGSYRCDCKAGFQRDAFGRGCIDVNECWASPGRLCQHTCENTLGSYRCSCASGFLLAADGKRCEDIDECAQGAGILCTFRCLNVPGSYQCACPEQGYTMTANGRSCKDVDECALGTHNCSEAETCHNIQGSFRCLRFECPPNYVQVSKTKCERTTCHDFLECQNSPARITHYQLNFQTGLLVPAHIFRIGPAPAFTGDTIALNIIKGNEEGYFGTRRLNAYTGVVYLQRAVLEPRDFALDVEMKLWRQGSVTTFLAKMHIFFTTFAL

Target ORF information:

RefSeq Version	XM_006713026.2
Organism	Homo sapiens(human)
Definition	Homo sapiens fibulin 2 (FBLN2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713026.2

ORF Insert Sequence:

ATGGTGCTGC TCTGGGAGCC TGCAGGAGCC TGGCTTGCTC TGGGCCTGGC CCTGGCCCTG 
GGCCCCAGCG TGGCCGCAGC TGCCCCTCGG CAGGACTGCA CGGGCGTGGA GTGCCCGCCG 
CTGGAGAACT GCATTGAGGA GGCGCTGGAG CCGGGTGCCT GCTGTGCCAC GTGTGTGCAG 
CAGGGCTGCG CCTGCGAGGG CTACCAGTAC TATGACTGCC TACAGGGTGG CTTCGTGCGC 
GGCCGCGTGC CCGCCGGTCA GTCCTATTTT GTGGACTTCG GGAGCACTGA GTGCTCCTGC 
CCACCAGGCG GCGGCAAGAT CAGCTGCCAG TTCATGCTGT GCCCGGAGCT GCCGCCCAAC 
TGCATCGAGG CTGTAGTGGT GGCTGACAGC TGCCCACAGT GCGGCCAGGT GGGCTGCGTC 
CACGCGGGCC ACAAGTACGC CGCTGGCCAC ACTGTTCACC TGCCGCCCTG CCGGGCCTGC 
CACTGCCCTG ACGCCGGTGG AGAGCTCATC TGCTACCAGC TCCCCGGTTG CCACGGGAAC 
TTCTCAGATG CCGAGGAGGG TGACCCCGAG CGACACTACG AAGACCCCTA CAGCTATGAC 
CAGGAGGTGG CCGAGGTGGA AGCAGCAACA GCCCTGGGGG GTGAGGTCCA GGCGGGTGCA 
GTCCAGGCAG GCGCAGGGGG CCCCCCAGCT GCTCTGGGAG GTGGGAGTCA GCCACTGTCC 
ACCATCCAGG CACCCCCCTG GCCAGCTGTC CTCCCCAGGC CCACAGCGGC TGCTGCCCTG 
GGTCCCCCAG CCCCAGTGCA GGCCAAAGCT AGGAGAGTGA CCGAGGACAG TGAGGAGGAA 
GAAGAGGAGG AGGAGGAGAG AGAGGAAATG GCTGTCACTG AGCAGCTGGC AGCAGGTGGC 
CACAGGGGGC TGGATGGGCT GCCCACTACA GCCCCAGCTG GACCCAGTCT TCCTATCCAG 
GAGGAGAGGG CAGAAGCTGG GGCAAGGGCA GAAGCTGGGG CAAGGCCTGA AGAGAACCTC 
ATCCTGGATG CCCAAGCCAC GTCCCGCAGC ACTGGGCCGG AGGGCGTGAC GCATGCACCG 
AGCCTGGGCA AGGCTGCTCT CGTCCCAACT CAGGCCGTGC CTGGCTCTCC CAGGGACCCA 
GTCAAGCCCA GCCCCCACAA CATCCTGTCC ACATCACTGC CTGATGCAGC CTGGATCCCA 
CCCACCCGAG AAGTGCCCAG GAAGCCGCAA GTTCTGCCCC ATTCCCACGT GGAGGAGGAC 
ACAGACCCCA ACTCTGTCCA TTCTATCCCC AGAAGTAGCC CTGAAGGCTC CACCAAGGAC 
CTGATCGAGA CTTGCTGCGC AGCCGGACAG CAGTGGGCCA TTGACAATGA CGAGTGCCTG 
GAGATCCCTG AGAGTGGCAC TGAGGACAAC GTCTGCAGGA CAGCCCAGAG GCACTGCTGT 
GTCTCCTACT TGCAGGAGAA GAGCTGCATG GCCGGCGTCC TGGGAGCCAA GGAGGGTGAG 
ACCTGTGGGG CTGAGGACAA CGACAGCTGC GGCATCTCCC TGTACAAGCA ATGCTGTGAC 
TGCTGTGGCC TGGGCCTCCG CGTGCGGGCC GAGGGCCAGT CGTGTGAGTC CAATCCTAAC 
CTGGGCTATC CCTGCAATCA TGTCATGCTC TCCTGCTGTG AGGGTGAAGA GCCTCTCATA 
GTACCTGAGG TTCGCCGACC TCCAGAGCCC GCAGCTGCAC CACGGAGAGT TTCAGAGGCA 
GAGATGGCGG GCCGAGAGGC CCTGTCACTG GGCACAGAGG CCGAGCTGCC GAACAGCCTG 
CCGGGCGATG ACCAGGATGA GTGCCTTCTC CTCCCGGGAG AGCTGTGCCA GCACCTTTGC 
ATCAATACTG TGGGTTCTTA CCACTGTGCC TGCTTTCCTG GCTTCTCACT GCAGGACGAT 
GGCCGCACTT GCCGCCCAGA GGGTCACCCT CCACAGCCGG AAGCCCCACA GGAGCCTGCA 
CTGAAGTCAG AATTTTCCCA GGTGGCCTCT AACACCATCC CGCTGCCACT GCCGCAGCCC 
AATACCTGCA AAGACAATGG ACCCTGCAAG CAGGTGTGCA GCACTGTTGG GGGCTCAGCC 
ATATGCTCCT GTTTTCCCGG CTATGCCATC ATGGCGGATG GCGTGTCCTG TGAAGACCAA 
GACGAGTGCC TGATGGGTGC TCACGATTGT AGCCGGCGAC AGTTCTGTGT GAACACCCTG 
GGATCCTTCT ACTGTGTCAA CCACACAGTG CTCTGTGCCG ATGGCTATAT CCTCAATGCG 
CACAGGAAGT GCGTGGACAT CAACGAGTGT GTGACGGACC TGCACACGTG CAGCCGGGGC 
GAGCACTGTG TGAACACACT GGGCTCCTTC CACTGCTACA AGGCACTCAC CTGTGAGCCA 
GGCTATGCCC TCAAGGATGG CGAGTGCGAA GACGTGGATG AGTGTGCGAT GGGCACGCAC 
ACCTGCCAGC CGGGCTTCTT GTGCCAGAAC ACCAAGGGCT CCTTCTACTG CCAGGCCAGG 
CAGCGCTGCA TGGATGGCTT CCTGCAGGAT CCTGAAGGCA ACTGTGTGGA CATCAACGAG 
TGCACGTCAC TGTCCGAGCC ATGTCGGCCA GGCTTCAGCT GCATCAACAC GGTGGGCTCC 
TACACATGCC AGAGGAACCC GCTGATCTGC GCGCGCGGCT ACCACGCCAG CGATGATGGG 
ACCAAGTGTG TGGACGTGAA TGAGTGTGAG ACAGGTGTGC ACCGCTGCGG TGAGGGCCAA 
GTGTGCCACA ACCTCCCTGG CTCCTACCGC TGTGACTGCA AAGCCGGCTT TCAGCGGGAT 
GCCTTTGGCC GGGGCTGCAT CGACGTGAAT GAGTGCTGGG CCTCGCCAGG CCGCCTGTGC 
CAGCACACGT GTGAGAACAC ACTCGGCTCC TACCGCTGTT CCTGCGCCTC CGGGTTCCTG 
CTAGCAGCGG ACGGCAAGCG CTGTGAAGAC ATCGACGAGT GTGCTCAAGG CGCCGGCATC 
CTCTGCACCT TCCGCTGTCT CAACGTGCCA GGGAGCTACC AGTGTGCATG CCCTGAGCAG 
GGCTACACCA TGACGGCCAA CGGGAGGTCC TGCAAGGACG TGGATGAGTG TGCACTGGGT 
ACCCACAACT GTTCCGAGGC TGAGACCTGC CACAACATCC AGGGTAGCTT CCGCTGCCTG 
CGCTTCGAGT GTCCTCCCAA CTATGTCCAA GTCTCCAAAA CGAAGTGCGA GCGCACCACG 
TGCCATGACT TCCTGGAGTG CCAGAACTCG CCAGCGCGCA TCACGCACTA CCAGCTCAAC 
TTCCAGACGG GCCTCCTGGT GCCTGCGCAT ATCTTCCGCA TTGGCCCCGC GCCAGCCTTC 
ACGGGGGACA CCATCGCCCT GAACATCATC AAGGGCAATG AGGAGGGCTA CTTTGGCACG 
CGCAGGCTCA ATGCCTACAC GGGTGTGGTC TACCTGCAGC GGGCCGTGCT GGAGCCCCGG 
GACTTTGCCC TGGACGTGGA GATGAAGCTC TGGAGGCAGG GCTCCGTCAC CACCTTCCTG 
GCCAAGATGC ACATCTTCTT CACCACCTTT GCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comprehensive proteomic characterization of stem cell-derived extracellular matrices.
Biomaterials128147-159(2017 06)
Ragelle H,Naba A,Larson BL,Zhou F,Priji? M,Whittaker CA,Del Rosario A,Langer R,Hynes RO,Anderson DG

Cleavage of Fibulin-2 by the aggrecanases ADAMTS-4 and ADAMTS-5 contributes to the tumorigenic potential of breast cancer cells.
Oncotarget8(8)13716-13729(2017 Feb)
Fontanil T,?lvarez-Teijeiro S,Villaronga M?,Mohamedi Y,Solares L,Moncada-Pazos A,Vega JA,Garc?a-Su?rez O,P?rez-Basterrechea M,Garc?a-Pedrero JM,Obaya AJ,Cal S

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

Extracellular matrix signatures of human primary metastatic colon cancers and their metastases to liver.
BMC cancer14518(2014 Jul)
Naba A,Clauser KR,Whittaker CA,Carr SA,Tanabe KK,Hynes RO

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FBLN2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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