CG31999 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG31999
Entrez Gene ID	43777
Full Name	CG31999 gene product from transcript CG31999-RC
Synonyms	CG10323,CG11288,CT31491,CT7856,Dmel\CG31999
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102A8-102B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001297783.1	NP_001284712.1	uncharacterized protein, isoform C
NM_166745.3	NP_726551.2	uncharacterized protein, isoform A

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	FBLN2	XP_425156.4
Drosophila melanogaster (fruit fly)	CG31999	NP_726551.2
Pan troglodytes (chimpanzee)	FBLN2	XP_003309683.1
Canis lupus familiaris (dog)	FBLN2	XP_541748.2
Mus musculus (house mouse)	Fbln2	NP_032018.2
Rattus norvegicus (Norway rat)	Fbln2	XP_006236985.1
Danio rerio (zebrafish)	fbln2	XP_001341277.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011322	XP_309330.4
Homo sapiens (human)	FBLN2	NP_001158507.1
Bos taurus (cattle)	FBLN2	XP_002697177.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG31999 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG31999 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00243	NM_166745.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG31999), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00243	NM_001297783.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG31999), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG31999 ( NM_001297783.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284712.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG31999), mRNA.

Price & Availability↑

CloneID	OFa00243
Clone ID Related Accession (Same CDS sequence)	NM_001297783.1 , NM_166745.3
Accession Version	NM_001297783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTTATA AACTATGTGC TTTTATGTGT TTCTTGATAG TTGGAGGGCA TGTCACCACC 
ACAATGGCCA GTGACAGCAT ATCGGGTTAT ATACGAAAAT GCTGTATAAA TGGACTTCGA 
CATGCTCGTA CGACCGCCTC TTGCAAAAAA ATCGACATAG CCCCAACAAT AATCCCGCAG 
CTCTGGCTTG GACTTTGTCA TTCTACACTT GAAGTCTGCT GTTCTCGAGA ACTGGACCAT 
CAAGATTGCG AGCTAGGTCG ACTGGCTGCT TTAGACGGTA CTCGCTGTGA CGGAGAAGGA 
AATGTAACAT CTAGCTCCTA TGCGACATGT TGTCGTTCTT GTCAAATCGG ATTAGCAGTT 
AAAGCGAGTA AAGCCAATTG CAAAGATCCA TTATTTTCAT TTATTTTCTT AATTGAATCA 
TACCGAGCGT GCTGTTACGG ATCTGCTGAC TTTAAAGACC AGCCCGGTAT CGACGAAATC 
GATAAGGCTA ATTCTATTAC TGATGAAGGT GAATTGCCGT TTGTTTCTGA AGAGGATATG 
AATGTAACTA TTGTTCTTAC CGGCGATGAT GACATATGTG GAAAAATTGA AAACCTTTGC 
GCTCACATAT GCGAAAACAC TTTCGACGCA TACCAATGTA AGTGCCACCC CGGGTTTATG 
TTAGATAATA ACAACGTAAC ATGCTCTCCG ATGAAAACCC AAATATGTCC CAGCGGTTAC 
AATTTAGATA AACTGGATAA CAAATGCATT GACATTGATG AATGCCGGGA AGATCTTCAT 
GACTGCAAAT CATCCCAATA TTGCCATAAT ACGAATGGCG GATACCACTG CTTAAATGTG 
AAAGAAAAAG AGTGTCCACC AGGATTTCAT TATGACCATG ATTACGATGC ATGTAAAGAT 
GATTACAAAT GCAAAGATCG TAAATGCGTT AAAATTCAAT CTTGTGATAA AGGATTCAGC 
TTGCATAACG GTACCTGTTC CGATATTGAC GAGTGCTCAC ATAAATCATT AAACAACTGT 
CATGTCAATA GTAATCAGGA ATGTGTTAAC ACAGTGGGAA GTTATTCCTG CAATTGCCTG 
CCTGGATTTA ATTTGGATGC TACACTAAAT AAGTGTGTTG ATATAAATGA ATGTTCAATA 
AATAATCACA ACTGCCTTCC AACTCAGAGA TGTGATAACA CGATCGGATC ATATATATGC 
ACGCGCCTAC AAAGCTGCGG AACAGGCTAT ACCTTAAATG CAGAAACGGG GAATTGTGAC 
GATGATGATG AATGCACCCT TAGTACACAT AACTGTCCGT CAAACTATGA TTGTCACAAC 
ACGAGGGGAT CATTCCGATG TTATAGAAAA ATCTCAACAA TGTTAACAAC AAGAACGACA 
TCAACTACAG TTCCGCCTTT ATCTTTGGAA AACGCACGAA GGAGTTTTAC TAGCCGATAC 
CCATACCCAT TAGCCGTACA TCCGGAATAC TCACAAAACA ACGATTCCAT TTCTACGAAT 
CGGCGTGTAG ACTGCTCCCC TGGATTTTAT AGGAATACTC TTGGTGCCTG CATAGATACA 
AATGAATGCA TGGAACAAAA TCCGTGCGGA AACCATGAAC GATGCATTAA CACAAATGGC 
CATTTCCGCT GCGAAAGTCT GCTCCAGTGC TCGCCCGGCT ACAAATCAAC AGTGGACGGT 
AAATCATGCA TAGACATTGA TGAGTGCGAT ACTGGTGAAC ACAATTGTGG CGAAAGACAG 
ATTTGCCGTA ATCGAAACGG TGGCTTTGTG TGCTCCTGCC CGATAGGTCA TGAGCTCAAA 
CGATCCATTG GTGGTGCTAG TACATGTGTT GACACGAACG AATGCGCTCT AGAACAACGT 
GTATGTCCAT TGAACGCCCA GTGCTTTAAC ACTATTGGAG CTTACTACTG CGAATGCAAA 
GCTGGCTTTC AAAAGAAATC TGATGGTAAT AATAGCACAC AATGTTTTGA TATCGATGAG 
TGTCAAGTGA TTCCGGGACT TTGTCAACAA AAATGTCTCA ACTTTTGGGG AGGATATCGA 
TGCACTTGTA ACTCTGGATA CCAATTGGGA CCGGACAACC GGACATGCAA TGATATAAAT 
GAATGCGAAG TACATAAGGA TTACAAACTA TGTATGGGAT TATGCATAAA CACTCCTGGT 
TCGTATCAAT GTTCCTGTCC ACGTGGATAT ATATTGGCTG CAGATATGAA TACGTGTCGT 
GATGTCGATG AATGCGCAAC AGATTCCATT AATCAAGTTT GTACTGGCCG GAATGACATT 
TGCACCAATA TCAGAGGAAG CTACAAATGT ACTACCGTTA ATTGTCCACT TGGATACAGC 
ATTGACCCAG AACAAAAAAA CCGGTGCCGC CAGAATCTTA ACTTTTGTGA AGGAGAAGAA 
TGTTACACCC AACCGTCAGC TTTCACATAT AATTTTATAA CATTCGTTTC TAAGCTAATG 
ATACCTCCAG ATGGTCGTAC CATATTTACA TTGAGAGGTC CACTTTGGTA TGATAATATC 
GAATTTGATC TTAAAATTGT TCGCATACAA GCAACCACCA ATATTCAAAA GGCAACCGAT 
GGGAGTTTTG ACACTTTACA AAACAACAAC CAAGTTAACG TGATATTAAA GAAATCACTG 
GAGGGACCCC AGGACATCGA GTTGGAGTTA AGTATGACAG TGTACACAAA CGGAATGCCT 
CGTGGAAAAA GTGTAGCAAA ATTATTTCTG TTTGTTTCTC AACATACATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284712.1
CDS	790..3543
Translation	MFYKLCAFMCFLIVGGHVTTTMASDSISGYIRKCCINGLRHARTTASCKKIDIAPTIIPQLWLGLCHSTLEVCCSRELDHQDCELGRLAALDGTRCDGEGNVTSSSYATCCRSCQIGLAVKASKANCKDPLFSFIFLIESYRACCYGSADFKDQPGIDEIDKANSITDEGELPFVSEEDMNVTIVLTGDDDICGKIENLCAHICENTFDAYQCKCHPGFMLDNNNVTCSPMKTQICPSGYNLDKLDNKCIDIDECREDLHDCKSSQYCHNTNGGYHCLNVKEKECPPGFHYDHDYDACKDDYKCKDRKCVKIQSCDKGFSLHNGTCSDIDECSHKSLNNCHVNSNQECVNTVGSYSCNCLPGFNLDATLNKCVDINECSINNHNCLPTQRCDNTIGSYICTRLQSCGTGYTLNAETGNCDDDDECTLSTHNCPSNYDCHNTRGSFRCYRKISTMLTTRTTSTTVPPLSLENARRSFTSRYPYPLAVHPEYSQNNDSISTNRRVDCSPGFYRNTLGACIDTNECMEQNPCGNHERCINTNGHFRCESLLQCSPGYKSTVDGKSCIDIDECDTGEHNCGERQICRNRNGGFVCSCPIGHELKRSIGGASTCVDTNECALEQRVCPLNAQCFNTIGAYYCECKAGFQKKSDGNNSTQCFDIDECQVIPGLCQQKCLNFWGGYRCTCNSGYQLGPDNRTCNDINECEVHKDYKLCMGLCINTPGSYQCSCPRGYILAADMNTCRDVDECATDSINQVCTGRNDICTNIRGSYKCTTVNCPLGYSIDPEQKNRCRQNLNFCEGEECYTQPSAFTYNFITFVSKLMIPPDGRTIFTLRGPLWYDNIEFDLKIVRIQATTNIQKATDGSFDTLQNNNQVNVILKKSLEGPQDIELELSMTVYTNGMPRGKSVAKLFLFVSQHTF

Target ORF information:

RefSeq Version	NM_001297783.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG31999), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297783.1

ORF Insert Sequence:

ATGTTTTATA AACTATGTGC TTTTATGTGT TTCTTGATAG TTGGAGGGCA TGTCACCACC 
ACAATGGCCA GTGACAGCAT ATCGGGTTAT ATACGAAAAT GCTGTATAAA TGGACTTCGA 
CATGCTCGTA CGACCGCCTC TTGCAAAAAA ATCGACATAG CCCCAACAAT AATCCCGCAG 
CTCTGGCTTG GACTTTGTCA TTCTACACTT GAAGTCTGCT GTTCTCGAGA ACTGGACCAT 
CAAGATTGCG AGCTAGGTCG ACTGGCTGCT TTAGACGGTA CTCGCTGTGA CGGAGAAGGA 
AATGTAACAT CTAGCTCCTA TGCGACATGT TGTCGTTCTT GTCAAATCGG ATTAGCAGTT 
AAAGCGAGTA AAGCCAATTG CAAAGATCCA TTATTTTCAT TTATTTTCTT AATTGAATCA 
TACCGAGCGT GCTGTTACGG ATCTGCTGAC TTTAAAGACC AGCCCGGTAT CGACGAAATC 
GATAAGGCTA ATTCTATTAC TGATGAAGGT GAATTGCCGT TTGTTTCTGA AGAGGATATG 
AATGTAACTA TTGTTCTTAC CGGCGATGAT GACATATGTG GAAAAATTGA AAACCTTTGC 
GCTCACATAT GCGAAAACAC TTTCGACGCA TACCAATGTA AGTGCCACCC CGGGTTTATG 
TTAGATAATA ACAACGTAAC ATGCTCTCCG ATGAAAACCC AAATATGTCC CAGCGGTTAC 
AATTTAGATA AACTGGATAA CAAATGCATT GACATTGATG AATGCCGGGA AGATCTTCAT 
GACTGCAAAT CATCCCAATA TTGCCATAAT ACGAATGGCG GATACCACTG CTTAAATGTG 
AAAGAAAAAG AGTGTCCACC AGGATTTCAT TATGACCATG ATTACGATGC ATGTAAAGAT 
GATTACAAAT GCAAAGATCG TAAATGCGTT AAAATTCAAT CTTGTGATAA AGGATTCAGC 
TTGCATAACG GTACCTGTTC CGATATTGAC GAGTGCTCAC ATAAATCATT AAACAACTGT 
CATGTCAATA GTAATCAGGA ATGTGTTAAC ACAGTGGGAA GTTATTCCTG CAATTGCCTG 
CCTGGATTTA ATTTGGATGC TACACTAAAT AAGTGTGTTG ATATAAATGA ATGTTCAATA 
AATAATCACA ACTGCCTTCC AACTCAGAGA TGTGATAACA CGATCGGATC ATATATATGC 
ACGCGCCTAC AAAGCTGCGG AACAGGCTAT ACCTTAAATG CAGAAACGGG GAATTGTGAC 
GATGATGATG AATGCACCCT TAGTACACAT AACTGTCCGT CAAACTATGA TTGTCACAAC 
ACGAGGGGAT CATTCCGATG TTATAGAAAA ATCTCAACAA TGTTAACAAC AAGAACGACA 
TCAACTACAG TTCCGCCTTT ATCTTTGGAA AACGCACGAA GGAGTTTTAC TAGCCGATAC 
CCATACCCAT TAGCCGTACA TCCGGAATAC TCACAAAACA ACGATTCCAT TTCTACGAAT 
CGGCGTGTAG ACTGCTCCCC TGGATTTTAT AGGAATACTC TTGGTGCCTG CATAGATACA 
AATGAATGCA TGGAACAAAA TCCGTGCGGA AACCATGAAC GATGCATTAA CACAAATGGC 
CATTTCCGCT GCGAAAGTCT GCTCCAGTGC TCGCCCGGCT ACAAATCAAC AGTGGACGGT 
AAATCATGCA TAGACATTGA TGAGTGCGAT ACTGGTGAAC ACAATTGTGG CGAAAGACAG 
ATTTGCCGTA ATCGAAACGG TGGCTTTGTG TGCTCCTGCC CGATAGGTCA TGAGCTCAAA 
CGATCCATTG GTGGTGCTAG TACATGTGTT GACACGAACG AATGCGCTCT AGAACAACGT 
GTATGTCCAT TGAACGCCCA GTGCTTTAAC ACTATTGGAG CTTACTACTG CGAATGCAAA 
GCTGGCTTTC AAAAGAAATC TGATGGTAAT AATAGCACAC AATGTTTTGA TATCGATGAG 
TGTCAAGTGA TTCCGGGACT TTGTCAACAA AAATGTCTCA ACTTTTGGGG AGGATATCGA 
TGCACTTGTA ACTCTGGATA CCAATTGGGA CCGGACAACC GGACATGCAA TGATATAAAT 
GAATGCGAAG TACATAAGGA TTACAAACTA TGTATGGGAT TATGCATAAA CACTCCTGGT 
TCGTATCAAT GTTCCTGTCC ACGTGGATAT ATATTGGCTG CAGATATGAA TACGTGTCGT 
GATGTCGATG AATGCGCAAC AGATTCCATT AATCAAGTTT GTACTGGCCG GAATGACATT 
TGCACCAATA TCAGAGGAAG CTACAAATGT ACTACCGTTA ATTGTCCACT TGGATACAGC 
ATTGACCCAG AACAAAAAAA CCGGTGCCGC CAGAATCTTA ACTTTTGTGA AGGAGAAGAA 
TGTTACACCC AACCGTCAGC TTTCACATAT AATTTTATAA CATTCGTTTC TAAGCTAATG 
ATACCTCCAG ATGGTCGTAC CATATTTACA TTGAGAGGTC CACTTTGGTA TGATAATATC 
GAATTTGATC TTAAAATTGT TCGCATACAA GCAACCACCA ATATTCAAAA GGCAACCGAT 
GGGAGTTTTG ACACTTTACA AAACAACAAC CAAGTTAACG TGATATTAAA GAAATCACTG 
GAGGGACCCC AGGACATCGA GTTGGAGTTA AGTATGACAG TGTACACAAA CGGAATGCCT 
CGTGGAAAAA GTGTAGCAAA ATTATTTCTG TTTGTTTCTC AACATACATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG31999 ( NM_166745.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726551.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG31999), mRNA.

Price & Availability↑

CloneID	OFa00243
Clone ID Related Accession (Same CDS sequence)	NM_001297783.1 , NM_166745.3
Accession Version	NM_166745.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166745.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTTATA AACTATGTGC TTTTATGTGT TTCTTGATAG TTGGAGGGCA TGTCACCACC 
ACAATGGCCA GTGACAGCAT ATCGGGTTAT ATACGAAAAT GCTGTATAAA TGGACTTCGA 
CATGCTCGTA CGACCGCCTC TTGCAAAAAA ATCGACATAG CCCCAACAAT AATCCCGCAG 
CTCTGGCTTG GACTTTGTCA TTCTACACTT GAAGTCTGCT GTTCTCGAGA ACTGGACCAT 
CAAGATTGCG AGCTAGGTCG ACTGGCTGCT TTAGACGGTA CTCGCTGTGA CGGAGAAGGA 
AATGTAACAT CTAGCTCCTA TGCGACATGT TGTCGTTCTT GTCAAATCGG ATTAGCAGTT 
AAAGCGAGTA AAGCCAATTG CAAAGATCCA TTATTTTCAT TTATTTTCTT AATTGAATCA 
TACCGAGCGT GCTGTTACGG ATCTGCTGAC TTTAAAGACC AGCCCGGTAT CGACGAAATC 
GATAAGGCTA ATTCTATTAC TGATGAAGGT GAATTGCCGT TTGTTTCTGA AGAGGATATG 
AATGTAACTA TTGTTCTTAC CGGCGATGAT GACATATGTG GAAAAATTGA AAACCTTTGC 
GCTCACATAT GCGAAAACAC TTTCGACGCA TACCAATGTA AGTGCCACCC CGGGTTTATG 
TTAGATAATA ACAACGTAAC ATGCTCTCCG ATGAAAACCC AAATATGTCC CAGCGGTTAC 
AATTTAGATA AACTGGATAA CAAATGCATT GACATTGATG AATGCCGGGA AGATCTTCAT 
GACTGCAAAT CATCCCAATA TTGCCATAAT ACGAATGGCG GATACCACTG CTTAAATGTG 
AAAGAAAAAG AGTGTCCACC AGGATTTCAT TATGACCATG ATTACGATGC ATGTAAAGAT 
GATTACAAAT GCAAAGATCG TAAATGCGTT AAAATTCAAT CTTGTGATAA AGGATTCAGC 
TTGCATAACG GTACCTGTTC CGATATTGAC GAGTGCTCAC ATAAATCATT AAACAACTGT 
CATGTCAATA GTAATCAGGA ATGTGTTAAC ACAGTGGGAA GTTATTCCTG CAATTGCCTG 
CCTGGATTTA ATTTGGATGC TACACTAAAT AAGTGTGTTG ATATAAATGA ATGTTCAATA 
AATAATCACA ACTGCCTTCC AACTCAGAGA TGTGATAACA CGATCGGATC ATATATATGC 
ACGCGCCTAC AAAGCTGCGG AACAGGCTAT ACCTTAAATG CAGAAACGGG GAATTGTGAC 
GATGATGATG AATGCACCCT TAGTACACAT AACTGTCCGT CAAACTATGA TTGTCACAAC 
ACGAGGGGAT CATTCCGATG TTATAGAAAA ATCTCAACAA TGTTAACAAC AAGAACGACA 
TCAACTACAG TTCCGCCTTT ATCTTTGGAA AACGCACGAA GGAGTTTTAC TAGCCGATAC 
CCATACCCAT TAGCCGTACA TCCGGAATAC TCACAAAACA ACGATTCCAT TTCTACGAAT 
CGGCGTGTAG ACTGCTCCCC TGGATTTTAT AGGAATACTC TTGGTGCCTG CATAGATACA 
AATGAATGCA TGGAACAAAA TCCGTGCGGA AACCATGAAC GATGCATTAA CACAAATGGC 
CATTTCCGCT GCGAAAGTCT GCTCCAGTGC TCGCCCGGCT ACAAATCAAC AGTGGACGGT 
AAATCATGCA TAGACATTGA TGAGTGCGAT ACTGGTGAAC ACAATTGTGG CGAAAGACAG 
ATTTGCCGTA ATCGAAACGG TGGCTTTGTG TGCTCCTGCC CGATAGGTCA TGAGCTCAAA 
CGATCCATTG GTGGTGCTAG TACATGTGTT GACACGAACG AATGCGCTCT AGAACAACGT 
GTATGTCCAT TGAACGCCCA GTGCTTTAAC ACTATTGGAG CTTACTACTG CGAATGCAAA 
GCTGGCTTTC AAAAGAAATC TGATGGTAAT AATAGCACAC AATGTTTTGA TATCGATGAG 
TGTCAAGTGA TTCCGGGACT TTGTCAACAA AAATGTCTCA ACTTTTGGGG AGGATATCGA 
TGCACTTGTA ACTCTGGATA CCAATTGGGA CCGGACAACC GGACATGCAA TGATATAAAT 
GAATGCGAAG TACATAAGGA TTACAAACTA TGTATGGGAT TATGCATAAA CACTCCTGGT 
TCGTATCAAT GTTCCTGTCC ACGTGGATAT ATATTGGCTG CAGATATGAA TACGTGTCGT 
GATGTCGATG AATGCGCAAC AGATTCCATT AATCAAGTTT GTACTGGCCG GAATGACATT 
TGCACCAATA TCAGAGGAAG CTACAAATGT ACTACCGTTA ATTGTCCACT TGGATACAGC 
ATTGACCCAG AACAAAAAAA CCGGTGCCGC CAGAATCTTA ACTTTTGTGA AGGAGAAGAA 
TGTTACACCC AACCGTCAGC TTTCACATAT AATTTTATAA CATTCGTTTC TAAGCTAATG 
ATACCTCCAG ATGGTCGTAC CATATTTACA TTGAGAGGTC CACTTTGGTA TGATAATATC 
GAATTTGATC TTAAAATTGT TCGCATACAA GCAACCACCA ATATTCAAAA GGCAACCGAT 
GGGAGTTTTG ACACTTTACA AAACAACAAC CAAGTTAACG TGATATTAAA GAAATCACTG 
GAGGGACCCC AGGACATCGA GTTGGAGTTA AGTATGACAG TGTACACAAA CGGAATGCCT 
CGTGGAAAAA GTGTAGCAAA ATTATTTCTG TTTGTTTCTC AACATACATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726551.2
CDS	123..2876
Translation	MFYKLCAFMCFLIVGGHVTTTMASDSISGYIRKCCINGLRHARTTASCKKIDIAPTIIPQLWLGLCHSTLEVCCSRELDHQDCELGRLAALDGTRCDGEGNVTSSSYATCCRSCQIGLAVKASKANCKDPLFSFIFLIESYRACCYGSADFKDQPGIDEIDKANSITDEGELPFVSEEDMNVTIVLTGDDDICGKIENLCAHICENTFDAYQCKCHPGFMLDNNNVTCSPMKTQICPSGYNLDKLDNKCIDIDECREDLHDCKSSQYCHNTNGGYHCLNVKEKECPPGFHYDHDYDACKDDYKCKDRKCVKIQSCDKGFSLHNGTCSDIDECSHKSLNNCHVNSNQECVNTVGSYSCNCLPGFNLDATLNKCVDINECSINNHNCLPTQRCDNTIGSYICTRLQSCGTGYTLNAETGNCDDDDECTLSTHNCPSNYDCHNTRGSFRCYRKISTMLTTRTTSTTVPPLSLENARRSFTSRYPYPLAVHPEYSQNNDSISTNRRVDCSPGFYRNTLGACIDTNECMEQNPCGNHERCINTNGHFRCESLLQCSPGYKSTVDGKSCIDIDECDTGEHNCGERQICRNRNGGFVCSCPIGHELKRSIGGASTCVDTNECALEQRVCPLNAQCFNTIGAYYCECKAGFQKKSDGNNSTQCFDIDECQVIPGLCQQKCLNFWGGYRCTCNSGYQLGPDNRTCNDINECEVHKDYKLCMGLCINTPGSYQCSCPRGYILAADMNTCRDVDECATDSINQVCTGRNDICTNIRGSYKCTTVNCPLGYSIDPEQKNRCRQNLNFCEGEECYTQPSAFTYNFITFVSKLMIPPDGRTIFTLRGPLWYDNIEFDLKIVRIQATTNIQKATDGSFDTLQNNNQVNVILKKSLEGPQDIELELSMTVYTNGMPRGKSVAKLFLFVSQHTF

Target ORF information:

RefSeq Version	NM_166745.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG31999), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166745.3

ORF Insert Sequence:

ATGTTTTATA AACTATGTGC TTTTATGTGT TTCTTGATAG TTGGAGGGCA TGTCACCACC 
ACAATGGCCA GTGACAGCAT ATCGGGTTAT ATACGAAAAT GCTGTATAAA TGGACTTCGA 
CATGCTCGTA CGACCGCCTC TTGCAAAAAA ATCGACATAG CCCCAACAAT AATCCCGCAG 
CTCTGGCTTG GACTTTGTCA TTCTACACTT GAAGTCTGCT GTTCTCGAGA ACTGGACCAT 
CAAGATTGCG AGCTAGGTCG ACTGGCTGCT TTAGACGGTA CTCGCTGTGA CGGAGAAGGA 
AATGTAACAT CTAGCTCCTA TGCGACATGT TGTCGTTCTT GTCAAATCGG ATTAGCAGTT 
AAAGCGAGTA AAGCCAATTG CAAAGATCCA TTATTTTCAT TTATTTTCTT AATTGAATCA 
TACCGAGCGT GCTGTTACGG ATCTGCTGAC TTTAAAGACC AGCCCGGTAT CGACGAAATC 
GATAAGGCTA ATTCTATTAC TGATGAAGGT GAATTGCCGT TTGTTTCTGA AGAGGATATG 
AATGTAACTA TTGTTCTTAC CGGCGATGAT GACATATGTG GAAAAATTGA AAACCTTTGC 
GCTCACATAT GCGAAAACAC TTTCGACGCA TACCAATGTA AGTGCCACCC CGGGTTTATG 
TTAGATAATA ACAACGTAAC ATGCTCTCCG ATGAAAACCC AAATATGTCC CAGCGGTTAC 
AATTTAGATA AACTGGATAA CAAATGCATT GACATTGATG AATGCCGGGA AGATCTTCAT 
GACTGCAAAT CATCCCAATA TTGCCATAAT ACGAATGGCG GATACCACTG CTTAAATGTG 
AAAGAAAAAG AGTGTCCACC AGGATTTCAT TATGACCATG ATTACGATGC ATGTAAAGAT 
GATTACAAAT GCAAAGATCG TAAATGCGTT AAAATTCAAT CTTGTGATAA AGGATTCAGC 
TTGCATAACG GTACCTGTTC CGATATTGAC GAGTGCTCAC ATAAATCATT AAACAACTGT 
CATGTCAATA GTAATCAGGA ATGTGTTAAC ACAGTGGGAA GTTATTCCTG CAATTGCCTG 
CCTGGATTTA ATTTGGATGC TACACTAAAT AAGTGTGTTG ATATAAATGA ATGTTCAATA 
AATAATCACA ACTGCCTTCC AACTCAGAGA TGTGATAACA CGATCGGATC ATATATATGC 
ACGCGCCTAC AAAGCTGCGG AACAGGCTAT ACCTTAAATG CAGAAACGGG GAATTGTGAC 
GATGATGATG AATGCACCCT TAGTACACAT AACTGTCCGT CAAACTATGA TTGTCACAAC 
ACGAGGGGAT CATTCCGATG TTATAGAAAA ATCTCAACAA TGTTAACAAC AAGAACGACA 
TCAACTACAG TTCCGCCTTT ATCTTTGGAA AACGCACGAA GGAGTTTTAC TAGCCGATAC 
CCATACCCAT TAGCCGTACA TCCGGAATAC TCACAAAACA ACGATTCCAT TTCTACGAAT 
CGGCGTGTAG ACTGCTCCCC TGGATTTTAT AGGAATACTC TTGGTGCCTG CATAGATACA 
AATGAATGCA TGGAACAAAA TCCGTGCGGA AACCATGAAC GATGCATTAA CACAAATGGC 
CATTTCCGCT GCGAAAGTCT GCTCCAGTGC TCGCCCGGCT ACAAATCAAC AGTGGACGGT 
AAATCATGCA TAGACATTGA TGAGTGCGAT ACTGGTGAAC ACAATTGTGG CGAAAGACAG 
ATTTGCCGTA ATCGAAACGG TGGCTTTGTG TGCTCCTGCC CGATAGGTCA TGAGCTCAAA 
CGATCCATTG GTGGTGCTAG TACATGTGTT GACACGAACG AATGCGCTCT AGAACAACGT 
GTATGTCCAT TGAACGCCCA GTGCTTTAAC ACTATTGGAG CTTACTACTG CGAATGCAAA 
GCTGGCTTTC AAAAGAAATC TGATGGTAAT AATAGCACAC AATGTTTTGA TATCGATGAG 
TGTCAAGTGA TTCCGGGACT TTGTCAACAA AAATGTCTCA ACTTTTGGGG AGGATATCGA 
TGCACTTGTA ACTCTGGATA CCAATTGGGA CCGGACAACC GGACATGCAA TGATATAAAT 
GAATGCGAAG TACATAAGGA TTACAAACTA TGTATGGGAT TATGCATAAA CACTCCTGGT 
TCGTATCAAT GTTCCTGTCC ACGTGGATAT ATATTGGCTG CAGATATGAA TACGTGTCGT 
GATGTCGATG AATGCGCAAC AGATTCCATT AATCAAGTTT GTACTGGCCG GAATGACATT 
TGCACCAATA TCAGAGGAAG CTACAAATGT ACTACCGTTA ATTGTCCACT TGGATACAGC 
ATTGACCCAG AACAAAAAAA CCGGTGCCGC CAGAATCTTA ACTTTTGTGA AGGAGAAGAA 
TGTTACACCC AACCGTCAGC TTTCACATAT AATTTTATAA CATTCGTTTC TAAGCTAATG 
ATACCTCCAG ATGGTCGTAC CATATTTACA TTGAGAGGTC CACTTTGGTA TGATAATATC 
GAATTTGATC TTAAAATTGT TCGCATACAA GCAACCACCA ATATTCAAAA GGCAACCGAT 
GGGAGTTTTG ACACTTTACA AAACAACAAC CAAGTTAACG TGATATTAAA GAAATCACTG 
GAGGGACCCC AGGACATCGA GTTGGAGTTA AGTATGACAG TGTACACAAA CGGAATGCCT 
CGTGGAAAAA GTGTAGCAAA ATTATTTCTG TTTGTTTCTC AACATACATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG31999 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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