Fbln2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fbln2
Entrez Gene ID	14115
Full Name	fibulin 2
Synonyms	5730577E14Rik,FIBL-2
General protein information	Preferred Names fibulin 2 Names fibulin-2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 D1\|6 40.42 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006505530.4	XP_006505593.1	fibulin-2 isoform X1
XM_030255156.1	XP_030111016.1	fibulin-2 isoform X2
NM_007992.2	NP_032018.2	fibulin-2 isoform a precursor
XM_030255157.1	XP_030111017.1	fibulin-2 isoform X3
XM_006505530.3	XP_006505593.1	fibulin-2 isoform X1
NM_001081437.1	NP_001074906.1	fibulin-2 isoform b precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	FBLN2	XP_425156.4
Drosophila melanogaster (fruit fly)	CG31999	NP_726551.2
Pan troglodytes (chimpanzee)	FBLN2	XP_003309683.1
Canis lupus familiaris (dog)	FBLN2	XP_541748.2
Mus musculus (house mouse)	Fbln2	NP_032018.2
Rattus norvegicus (Norway rat)	Fbln2	XP_006236985.1
Danio rerio (zebrafish)	fbln2	XP_001341277.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011322	XP_309330.4
Homo sapiens (human)	FBLN2	NP_001158507.1
Bos taurus (cattle)	FBLN2	XP_002697177.1

Gene Ontology
Bibliography

Related articles in PubMed

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Rutledge EA, Benazet JD, McMahon AP
Development (Cambridge, England)144(17)3177-3188(2017 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our study indicates that Ang II cannot induce TGF-beta activation without fibulin-2 and that fibulin-2 has an essential role in Ang II-induced TGF-beta signaling and subsequent myocardial fibrosis. Fibulin-2 can be considered as a critical regulator of TGF-beta that induces myocardial fibrosis.
Title: Fibulin-2 is essential for angiotensin II-induced myocardial fibrosis mediated by transforming growth factor (TGF)-?.

FBLN2 and VCAN were both preferentially expressed in the mammary gland during times of epithelial expansion in TEB-enriched tissue and co-localised specifically in cap cell.
Title: Fibulin-2 is involved in early extracellular matrix development of the outgrowing mouse mammary epithelium.

These findings demonstrate a prominent role for alpha3beta1 in immortalized/transformed keratinocytes in regulating fibulin-2 and other genes that promote matrix remodeling and invasion.
Title: Regulation of fibulin-2 gene expression by integrin ?3?1 contributes to the invasive phenotype of transformed keratinocytes.

integrin alpha3beta1 has a role in basement membrane stability through fibulin-2 induction, both in neonatal skin and in adult wounds
Title: Reduced fibulin-2 contributes to loss of basement membrane integrity and skin blistering in mice lacking integrin ?3?1 in the epidermis.

levels of fibulin 2 dramatically increased in the retinal pigment epithelium following retinal detachment, suggesting a direct role for fibulin 2 in the re-attachment of the retina to the retinal pigment epithelium.
Title: Fibulin 2, a tyrosine O-sulfated protein, is up-regulated following retinal detachment.

The following Fbln2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fbln2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01192	XM_006505530.4 Latest version!	Mus musculus fibulin 2 (Fbln2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu02441	XM_030255156.1 Latest version!	Mus musculus fibulin 2 (Fbln2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu89126	XM_030255157.1 Latest version!	Mus musculus fibulin 2 (Fbln2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu01192	NM_007992.2 Latest version!	Mus musculus fibulin 2 (Fbln2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu02441	NM_001081437.1 Latest version!	Mus musculus fibulin 2 (Fbln2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$692.30 $989.00
View Old Accession Versions >>
OMu01192	XM_006505530.3	Mus musculus fibulin 2 (Fbln2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fbln2 ( XM_006505530.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505593.1 Mus musculus fibulin 2 (Fbln2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu01192
Clone ID Related Accession (Same CDS sequence)	XM_006505530.4 , NM_007992.2 , XM_006505530.3
Accession Version	XM_006505530.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	fibulin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006505530.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCC AGGAATCTGC AGGAGTGTGG CTGGCCTTGG CTCTGGTCAC AGCTCTGACT 
CCGAGCCCAA GCATGGCCGT CCCGTGGCAG GATTGCACAG GTGCTGAGTG TCCCCTGCTG 
GAGAACTGCA TCGAGGAGGC GTTGGAGCCA GGAGCCTGCT GTGCTACCTG TGTGCAGCAG 
GGCTGTGCCT GCGAAGGCTA CCAGTACTAC GACTGCGTAC AAGGAGGGTT TGTGGATGGC 
CGTGTACCAG CCGGCCAGTC CTACTTTGTG GACTTTGGCA GCACCGAGTG CTCCTGCCCA 
CCCGGTGGTG GCAAGATCAG CTGCCAGTTC ATGCTGTGCC CTGAGCTACC ACCCAACTGC 
ATTGAAGCTG TAGTAGTAGC CGACAGCTGC CCGCAGTGTG GCCAGGTGGG CTGTGTCCAC 
AGTGGCCGTA AGTATGCTGC TGGCCATACC GTCCACCTCT CATCCTGCCG GGCTTGCCAC 
TGTCCTGACG CTGGTGGTGA GCTCATTTGC TACCAGCTTC CTGGTTGCCA TGGGAACTTC 
TCGGATGCTG AGGAGGGTGA CTCTGAGAGG CAGTATGAAG ACCCCTACAG CTATGACCAG 
GAGGTGGCAG AGGCAGAAGC TACTACGGCC ATTGTGAACG AGGTCCAGGC AGGTGCAGAG 
GGCCCCCCAG CTGCTCTGGG AGGAGGGAAT CTGCCACCGT CCTCCATCAG AGTAACACCC 
TGGCCAGTTG CCCTTCCCAG ACCCACAGCA GCTGCTGCCC TGGGTCCCCC AGCACCTGTG 
CAGGCCAAAG CCAGGAGAGT GACATTGGAC ACTGAAGAGG ATGAGGAAGA AGAAGAGGAG 
GAGACACTAG TCACTGAGCC ACCAACAGCA GGCAGTCCTG GAAGGCTGGA CAGCCTGCCC 
ACAAGATCCC CAGCCAGACC TGGTTTCCCT GTCCAGGAGA AGGAGGCTGA AGCTAAGGCA 
GGGCCTGAGG AAAACCTTAT TCCTGATGCC CAGGTCACTC CTCGAAGTGT CATGCAGGAG 
GGTGCTGCAC CTCTGCCAAG GTCAGGCCTG GCTGCTCTCA GTCCATCTCT GGCCACTGAC 
AGCTCTTCAG AGGACCCAGT AAAGCCCAGC GATCACCCCA CTCTATCTAC ACTGCCACCT 
GACCGAGCCC AGGTCTCCCC CTCCCCAGAG ACACCTGAAG AAATACCCCA GCATCCACAG 
TTGCTACCTC GATTCCGGGC AGAAGAAGAC ATAGACCCCA ACTCAGTACA TTCTGTCCCC 
AGAGGTGATC TTGATGGCTC CACCAAGGAC CTAATCGAGA CCTGTTGTGC AGCGGGCCAG 
CAGTGGGCCA TCGACAATGA TGAGTGCCAG GAGATCCCCG AGAATGGTGC CCAGAGTGAC 
ATCTGTAGGA TAGCCCAGAG GCAATGCTGC ATCTCCTACT TGAAAGAGAA GAGCTGTGTG 
GCTGGGGTCA TGGGAGCCAA GGAGGGTGAG ACCTGTGGAG CAGAGGACAA TGATACCTGC 
GGCGTCTCAC TCTACAAGCA ATGCTGTGAC TGCTGTGGCC TGGGACTCCG AGTGCGGGCT 
GAGGGCCAGT CCTGTGAGTC CAACCCCAAC CTGGGCTATC CCTGCAACCA TGTCATGCTC 
TCCTGCTGTG AAGGTGAAGA GCCCCTCATA GTGCCTGAGG TCCGCCGGCC ACCAGAGCCC 
GAGGCTGCTC CTCGCAGAGT TTCAGAGATG GAGATGGCAA GCCGGGAGGC CCTGTCACTG 
GGCACAGAGG CTGAACTGCC CAATAGCCTG CCGGGAGATG ACCAGGATGA GTGCCTGATG 
CTCCCTGGGG AGCTTTGCCA GCATCTTTGC ATCAACACCG TCGGCTCCTA CCGCTGTGCC 
TGCTTCCCTG GATTCGAGCT GCAGGGTGAT GGCCGTACCT GCCGCCCAGA TAGAGGTGCC 
CCACAGTTGG ACACTGCACG AGAGTCTGCA CCGAGGTCTG AGTCTGCCCA GGTATCTCCC 
AACACCATCC CACTGCCGGT GCCACAGCCC AACACCTGCA AAGACAATGG GCCCTGCCGT 
CAGGTGTGTC GTGTTGTTGG GGACACAGCT ATGTGCTCCT GCTTCCCTGG CTACGCCATC 
ATGGCAGATG GTGTGTCCTG TGAAGACCAA GACGAGTGTT TGATGGGCAC TCACGATTGT 
AGCTGGAAAC AGTTCTGTGT GAACACCCTG GGATCCTTCT ACTGTGTCAA CCACACAGTG 
CTGTGTGCAG AGGGCTATAT CCTCAACGCA CATAGGAAGT GTGTGGACAT CAATGAGTGT 
GTGACAGACC TGCACACGTG CACCCGGGCC GAGCACTGTG TGAACACGCC AGGTTCCTTC 
CAATGCTACA AGGCGCTCAC CTGTGAGCCA GGCTATGTCC TCACAGATGG TGAATGCACA 
GACGTGGACG AATGTGTGAC GGGCACACAC AACTGCCAGG CAGGCTTCTC ATGCCAGAAC 
ACCAAGGGCT CCTTCTACTG CCAGGCCCGG CAACGCTGCA TGGATGGCTT CTTGCAGGAC 
CCCGAGGGCA ACTGTGTGGA CATCAACGAA TGCACATCGT TACTGGAGCC TTGCCGCTCA 
GGGTTCAGCT GCATCAACAC AGTGGGCTCC TACACCTGTC AGAGGAACCC ACTGGTCTGC 
GGTCGCGGTT ACCATGCTAA CGAGGAGGGC TCTGAATGTG TGGATGTGAA TGAGTGTGAG 
ACAGGTGTGC ATCGCTGTGG CGAGGGCCAA CTGTGCTATA ACCTCCCTGG ATCCTACCGC 
TGTGACTGCA AGCCCGGCTT CCAGAGGGAT GCATTCGGCA GGACTTGCAT TGATGTGAAC 
GAATGCTGGG TCTCGCCGGG CCGCCTGTGC CAGCACACAT GTGAGAACAC ACCGGGCTCC 
TACCGCTGCT CCTGCGCTGC TGGCTTCCTT TTGGCCGCAG ATGGCAAACA TTGTGAAGAT 
GTGAACGAGT GCGAGACTCG GCGCTGCAGC CAGGAATGTG CCAACATCTA TGGCTCCTAT 
CAGTGCTACT GCCGTCAGGG CTACCAGCTG GCAGAGGATG GGCATACCTG CACAGACATC 
GATGAGTGTG CACAGGGCGC GGGCATTCTC TGTACCTTCC GCTGTGTCAA CGTGCCTGGG 
AGCTACCAGT GTGCATGCCC AGAGCAAGGG TATACAATGA TGGCCAACGG GAGGTCCTGC 
AAGGACCTGG ATGAGTGTGC ACTGGGCACC CACAACTGCT CTGAGGCTGA GACCTGCCAC 
AATATCCAGG GGAGTTTCCG CTGCCTGCGC TTTGATTGTC CACCCAACTA TGTCCGTGTC 
TCAGAAACGA AGTGCGAGCG CACCACATGC CAGGATATCA CGGAATGTCA AACCTCACCA 
GCTCGCATCA CGCACTACCA GCTCAATTTC CAGACAGGCC TACTGGTACC TGCACATATC 
TTCCGCATCG GCCCTGCTCC CGCCTTTGCT GGGGACACCA TCTCCCTGAC CATCACGAAG 
GGCAATGAGG AGGGCTACTT CGTCACACGC AGACTCAATG CCTACACTGG TGTGGTATCC 
CTGCAGCGGT CTGTTCTGGA GCCGCGGGAC TTTGCCCTAG ATGTGGAGAT GAAGCTTTGG 
CGCCAGGGCT CTGTCACTAC CTTCCTGGCC AAGATGTACA TCTTCTTCAC CACTTTTGCC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505593.1
CDS	306..3971
Translation	MLLQESAGVWLALALVTALTPSPSMAVPWQDCTGAECPLLENCIEEALEPGACCATCVQQGCACEGYQYYDCVQGGFVDGRVPAGQSYFVDFGSTECSCPPGGGKISCQFMLCPELPPNCIEAVVVADSCPQCGQVGCVHSGRKYAAGHTVHLSSCRACHCPDAGGELICYQLPGCHGNFSDAEEGDSERQYEDPYSYDQEVAEAEATTAIVNEVQAGAEGPPAALGGGNLPPSSIRVTPWPVALPRPTAAAALGPPAPVQAKARRVTLDTEEDEEEEEEETLVTEPPTAGSPGRLDSLPTRSPARPGFPVQEKEAEAKAGPEENLIPDAQVTPRSVMQEGAAPLPRSGLAALSPSLATDSSSEDPVKPSDHPTLSTLPPDRAQVSPSPETPEEIPQHPQLLPRFRAEEDIDPNSVHSVPRGDLDGSTKDLIETCCAAGQQWAIDNDECQEIPENGAQSDICRIAQRQCCISYLKEKSCVAGVMGAKEGETCGAEDNDTCGVSLYKQCCDCCGLGLRVRAEGQSCESNPNLGYPCNHVMLSCCEGEEPLIVPEVRRPPEPEAAPRRVSEMEMASREALSLGTEAELPNSLPGDDQDECLMLPGELCQHLCINTVGSYRCACFPGFELQGDGRTCRPDRGAPQLDTARESAPRSESAQVSPNTIPLPVPQPNTCKDNGPCRQVCRVVGDTAMCSCFPGYAIMADGVSCEDQDECLMGTHDCSWKQFCVNTLGSFYCVNHTVLCAEGYILNAHRKCVDINECVTDLHTCTRAEHCVNTPGSFQCYKALTCEPGYVLTDGECTDVDECVTGTHNCQAGFSCQNTKGSFYCQARQRCMDGFLQDPEGNCVDINECTSLLEPCRSGFSCINTVGSYTCQRNPLVCGRGYHANEEGSECVDVNECETGVHRCGEGQLCYNLPGSYRCDCKPGFQRDAFGRTCIDVNECWVSPGRLCQHTCENTPGSYRCSCAAGFLLAADGKHCEDVNECETRRCSQECANIYGSYQCYCRQGYQLAEDGHTCTDIDECAQGAGILCTFRCVNVPGSYQCACPEQGYTMMANGRSCKDLDECALGTHNCSEAETCHNIQGSFRCLRFDCPPNYVRVSETKCERTTCQDITECQTSPARITHYQLNFQTGLLVPAHIFRIGPAPAFAGDTISLTITKGNEEGYFVTRRLNAYTGVVSLQRSVLEPRDFALDVEMKLWRQGSVTTFLAKMYIFFTTFAP

Target ORF information:

RefSeq Version	XM_006505530.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus fibulin 2 (Fbln2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505530.4

ORF Insert Sequence:

ATGCTGCTCC AGGAATCTGC AGGAGTGTGG CTGGCCTTGG CTCTGGTCAC AGCTCTGACT 
CCGAGCCCAA GCATGGCCGT CCCGTGGCAG GATTGCACAG GTGCTGAGTG TCCCCTGCTG 
GAGAACTGCA TCGAGGAGGC GTTGGAGCCA GGAGCCTGCT GTGCTACCTG TGTGCAGCAG 
GGCTGTGCCT GCGAAGGCTA CCAGTACTAC GACTGCGTAC AAGGAGGGTT TGTGGATGGC 
CGTGTACCAG CCGGCCAGTC CTACTTTGTG GACTTTGGCA GCACCGAGTG CTCCTGCCCA 
CCCGGTGGTG GCAAGATCAG CTGCCAGTTC ATGCTGTGCC CTGAGCTACC ACCCAACTGC 
ATTGAAGCTG TAGTAGTAGC CGACAGCTGC CCGCAGTGTG GCCAGGTGGG CTGTGTCCAC 
AGTGGCCGTA AGTATGCTGC TGGCCATACC GTCCACCTCT CATCCTGCCG GGCTTGCCAC 
TGTCCTGACG CTGGTGGTGA GCTCATTTGC TACCAGCTTC CTGGTTGCCA TGGGAACTTC 
TCGGATGCTG AGGAGGGTGA CTCTGAGAGG CAGTATGAAG ACCCCTACAG CTATGACCAG 
GAGGTGGCAG AGGCAGAAGC TACTACGGCC ATTGTGAACG AGGTCCAGGC AGGTGCAGAG 
GGCCCCCCAG CTGCTCTGGG AGGAGGGAAT CTGCCACCGT CCTCCATCAG AGTAACACCC 
TGGCCAGTTG CCCTTCCCAG ACCCACAGCA GCTGCTGCCC TGGGTCCCCC AGCACCTGTG 
CAGGCCAAAG CCAGGAGAGT GACATTGGAC ACTGAAGAGG ATGAGGAAGA AGAAGAGGAG 
GAGACACTAG TCACTGAGCC ACCAACAGCA GGCAGTCCTG GAAGGCTGGA CAGCCTGCCC 
ACAAGATCCC CAGCCAGACC TGGTTTCCCT GTCCAGGAGA AGGAGGCTGA AGCTAAGGCA 
GGGCCTGAGG AAAACCTTAT TCCTGATGCC CAGGTCACTC CTCGAAGTGT CATGCAGGAG 
GGTGCTGCAC CTCTGCCAAG GTCAGGCCTG GCTGCTCTCA GTCCATCTCT GGCCACTGAC 
AGCTCTTCAG AGGACCCAGT AAAGCCCAGC GATCACCCCA CTCTATCTAC ACTGCCACCT 
GACCGAGCCC AGGTCTCCCC CTCCCCAGAG ACACCTGAAG AAATACCCCA GCATCCACAG 
TTGCTACCTC GATTCCGGGC AGAAGAAGAC ATAGACCCCA ACTCAGTACA TTCTGTCCCC 
AGAGGTGATC TTGATGGCTC CACCAAGGAC CTAATCGAGA CCTGTTGTGC AGCGGGCCAG 
CAGTGGGCCA TCGACAATGA TGAGTGCCAG GAGATCCCCG AGAATGGTGC CCAGAGTGAC 
ATCTGTAGGA TAGCCCAGAG GCAATGCTGC ATCTCCTACT TGAAAGAGAA GAGCTGTGTG 
GCTGGGGTCA TGGGAGCCAA GGAGGGTGAG ACCTGTGGAG CAGAGGACAA TGATACCTGC 
GGCGTCTCAC TCTACAAGCA ATGCTGTGAC TGCTGTGGCC TGGGACTCCG AGTGCGGGCT 
GAGGGCCAGT CCTGTGAGTC CAACCCCAAC CTGGGCTATC CCTGCAACCA TGTCATGCTC 
TCCTGCTGTG AAGGTGAAGA GCCCCTCATA GTGCCTGAGG TCCGCCGGCC ACCAGAGCCC 
GAGGCTGCTC CTCGCAGAGT TTCAGAGATG GAGATGGCAA GCCGGGAGGC CCTGTCACTG 
GGCACAGAGG CTGAACTGCC CAATAGCCTG CCGGGAGATG ACCAGGATGA GTGCCTGATG 
CTCCCTGGGG AGCTTTGCCA GCATCTTTGC ATCAACACCG TCGGCTCCTA CCGCTGTGCC 
TGCTTCCCTG GATTCGAGCT GCAGGGTGAT GGCCGTACCT GCCGCCCAGA TAGAGGTGCC 
CCACAGTTGG ACACTGCACG AGAGTCTGCA CCGAGGTCTG AGTCTGCCCA GGTATCTCCC 
AACACCATCC CACTGCCGGT GCCACAGCCC AACACCTGCA AAGACAATGG GCCCTGCCGT 
CAGGTGTGTC GTGTTGTTGG GGACACAGCT ATGTGCTCCT GCTTCCCTGG CTACGCCATC 
ATGGCAGATG GTGTGTCCTG TGAAGACCAA GACGAGTGTT TGATGGGCAC TCACGATTGT 
AGCTGGAAAC AGTTCTGTGT GAACACCCTG GGATCCTTCT ACTGTGTCAA CCACACAGTG 
CTGTGTGCAG AGGGCTATAT CCTCAACGCA CATAGGAAGT GTGTGGACAT CAATGAGTGT 
GTGACAGACC TGCACACGTG CACCCGGGCC GAGCACTGTG TGAACACGCC AGGTTCCTTC 
CAATGCTACA AGGCGCTCAC CTGTGAGCCA GGCTATGTCC TCACAGATGG TGAATGCACA 
GACGTGGACG AATGTGTGAC GGGCACACAC AACTGCCAGG CAGGCTTCTC ATGCCAGAAC 
ACCAAGGGCT CCTTCTACTG CCAGGCCCGG CAACGCTGCA TGGATGGCTT CTTGCAGGAC 
CCCGAGGGCA ACTGTGTGGA CATCAACGAA TGCACATCGT TACTGGAGCC TTGCCGCTCA 
GGGTTCAGCT GCATCAACAC AGTGGGCTCC TACACCTGTC AGAGGAACCC ACTGGTCTGC 
GGTCGCGGTT ACCATGCTAA CGAGGAGGGC TCTGAATGTG TGGATGTGAA TGAGTGTGAG 
ACAGGTGTGC ATCGCTGTGG CGAGGGCCAA CTGTGCTATA ACCTCCCTGG ATCCTACCGC 
TGTGACTGCA AGCCCGGCTT CCAGAGGGAT GCATTCGGCA GGACTTGCAT TGATGTGAAC 
GAATGCTGGG TCTCGCCGGG CCGCCTGTGC CAGCACACAT GTGAGAACAC ACCGGGCTCC 
TACCGCTGCT CCTGCGCTGC TGGCTTCCTT TTGGCCGCAG ATGGCAAACA TTGTGAAGAT 
GTGAACGAGT GCGAGACTCG GCGCTGCAGC CAGGAATGTG CCAACATCTA TGGCTCCTAT 
CAGTGCTACT GCCGTCAGGG CTACCAGCTG GCAGAGGATG GGCATACCTG CACAGACATC 
GATGAGTGTG CACAGGGCGC GGGCATTCTC TGTACCTTCC GCTGTGTCAA CGTGCCTGGG 
AGCTACCAGT GTGCATGCCC AGAGCAAGGG TATACAATGA TGGCCAACGG GAGGTCCTGC 
AAGGACCTGG ATGAGTGTGC ACTGGGCACC CACAACTGCT CTGAGGCTGA GACCTGCCAC 
AATATCCAGG GGAGTTTCCG CTGCCTGCGC TTTGATTGTC CACCCAACTA TGTCCGTGTC 
TCAGAAACGA AGTGCGAGCG CACCACATGC CAGGATATCA CGGAATGTCA AACCTCACCA 
GCTCGCATCA CGCACTACCA GCTCAATTTC CAGACAGGCC TACTGGTACC TGCACATATC 
TTCCGCATCG GCCCTGCTCC CGCCTTTGCT GGGGACACCA TCTCCCTGAC CATCACGAAG 
GGCAATGAGG AGGGCTACTT CGTCACACGC AGACTCAATG CCTACACTGG TGTGGTATCC 
CTGCAGCGGT CTGTTCTGGA GCCGCGGGAC TTTGCCCTAG ATGTGGAGAT GAAGCTTTGG 
CGCCAGGGCT CTGTCACTAC CTTCCTGGCC AAGATGTACA TCTTCTTCAC CACTTTTGCC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fbln2 ( XM_030255156.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030111016.1 Mus musculus fibulin 2 (Fbln2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu02441
Clone ID Related Accession (Same CDS sequence)	XM_030255156.1 , NM_001081437.1
Accession Version	XM_030255156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	fibulin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCC AGGAATCTGC AGGAGTGTGG CTGGCCTTGG CTCTGGTCAC AGCTCTGACT 
CCGAGCCCAA GCATGGCCGT CCCGTGGCAG GATTGCACAG GTGCTGAGTG TCCCCTGCTG 
GAGAACTGCA TCGAGGAGGC GTTGGAGCCA GGAGCCTGCT GTGCTACCTG TGTGCAGCAG 
GGCTGTGCCT GCGAAGGCTA CCAGTACTAC GACTGCGTAC AAGGAGGGTT TGTGGATGGC 
CGTGTACCAG CCGGCCAGTC CTACTTTGTG GACTTTGGCA GCACCGAGTG CTCCTGCCCA 
CCCGGTGGTG GCAAGATCAG CTGCCAGTTC ATGCTGTGCC CTGAGCTACC ACCCAACTGC 
ATTGAAGCTG TAGTAGTAGC CGACAGCTGC CCGCAGTGTG GCCAGGTGGG CTGTGTCCAC 
AGTGGCCGTA AGTATGCTGC TGGCCATACC GTCCACCTCT CATCCTGCCG GGCTTGCCAC 
TGTCCTGACG CTGGTGGTGA GCTCATTTGC TACCAGCTTC CTGGTTGCCA TGGGAACTTC 
TCGGATGCTG AGGAGGGTGA CTCTGAGAGG CAGTATGAAG ACCCCTACAG CTATGACCAG 
GAGGTGGCAG AGGCAGAAGC TACTACGGCC ATTGTGAACG AGGTCCAGGC AGGTGCAGAG 
GGCCCCCCAG CTGCTCTGGG AGGAGGGAAT CTGCCACCGT CCTCCATCAG AGTAACACCC 
TGGCCAGTTG CCCTTCCCAG ACCCACAGCA GCTGCTGCCC TGGGTCCCCC AGCACCTGTG 
CAGGCCAAAG CCAGGAGAGT GACATTGGAC ACTGAAGAGG ATGAGGAAGA AGAAGAGGAG 
GAGACACTAG TCACTGAGCC ACCAACAGCA GGCAGTCCTG GAAGGCTGGA CAGCCTGCCC 
ACAAGATCCC CAGCCAGACC TGGTTTCCCT GTCCAGGAGA AGGAGGCTGA AGCTAAGGCA 
GGGCCTGAGG AAAACCTTAT TCCTGATGCC CAGGTCACTC CTCGAAGTGT CATGCAGGAG 
GGTGCTGCAC CTCTGCCAAG GTCAGGCCTG GCTGCTCTCA GTCCATCTCT GGCCACTGAC 
AGCTCTTCAG AGGACCCAGT AAAGCCCAGC GATCACCCCA CTCTATCTAC ACTGCCACCT 
GACCGAGCCC AGGTCTCCCC CTCCCCAGAG ACACCTGAAG AAATACCCCA GCATCCACAG 
TTGCTACCTC GATTCCGGGC AGAAGAAGAC ATAGACCCCA ACTCAGTACA TTCTGTCCCC 
AGAGGTGATC TTGATGGCTC CACCAAGGAC CTAATCGAGA CCTGTTGTGC AGCGGGCCAG 
CAGTGGGCCA TCGACAATGA TGAGTGCCAG GAGATCCCCG AGAATGGTGC CCAGAGTGAC 
ATCTGTAGGA TAGCCCAGAG GCAATGCTGC ATCTCCTACT TGAAAGAGAA GAGCTGTGTG 
GCTGGGGTCA TGGGAGCCAA GGAGGGTGAG ACCTGTGGAG CAGAGGACAA TGATACCTGC 
GGCGTCTCAC TCTACAAGCA ATGCTGTGAC TGCTGTGGCC TGGGACTCCG AGTGCGGGCT 
GAGGGCCAGT CCTGTGAGTC CAACCCCAAC CTGGGCTATC CCTGCAACCA TGTCATGCTC 
TCCTGCTGTG AAGGTGAAGA GCCCCTCATA GTGCCTGAGG TCCGCCGGCC ACCAGAGCCC 
GAGGCTGCTC CTCGCAGAGT TTCAGAGATG GAGATGGCAA GCCGGGAGGC CCTGTCACTG 
GGCACAGAGG CTGAACTGCC CAATAGCCTG CCGGGAGATG ACCAGGATGA GTGCCTGATG 
CTCCCTGGGG AGCTTTGCCA GCATCTTTGC ATCAACACCG TCGGCTCCTA CCGCTGTGCC 
TGCTTCCCTG GATTCGAGCT GCAGGGTGAT GGCCGTACCT GCCGCCCAGA TAGAGGTGCC 
CCACAGTTGG ACACTGCACG AGAGTCTGCA CCGAGGTCTG AGTCTGCCCA GGTATCTCCC 
AACACCATCC CACTGCCGGT GCCACAGCCC AACACCTGCA AAGACAATGG GCCCTGCCGT 
CAGGTGTGTC GTGTTGTTGG GGACACAGCT ATGTGCTCCT GCTTCCCTGG CTACGCCATC 
ATGGCAGATG GTGTGTCCTG TGAAGACATC AATGAGTGTG TGACAGACCT GCACACGTGC 
ACCCGGGCCG AGCACTGTGT GAACACGCCA GGTTCCTTCC AATGCTACAA GGCGCTCACC 
TGTGAGCCAG GCTATGTCCT CACAGATGGT GAATGCACAG ACGTGGACGA ATGTGTGACG 
GGCACACACA ACTGCCAGGC AGGCTTCTCA TGCCAGAACA CCAAGGGCTC CTTCTACTGC 
CAGGCCCGGC AACGCTGCAT GGATGGCTTC TTGCAGGACC CCGAGGGCAA CTGTGTGGAC 
ATCAACGAAT GCACATCGTT ACTGGAGCCT TGCCGCTCAG GGTTCAGCTG CATCAACACA 
GTGGGCTCCT ACACCTGTCA GAGGAACCCA CTGGTCTGCG GTCGCGGTTA CCATGCTAAC 
GAGGAGGGCT CTGAATGTGT GGATGTGAAT GAGTGTGAGA CAGGTGTGCA TCGCTGTGGC 
GAGGGCCAAC TGTGCTATAA CCTCCCTGGA TCCTACCGCT GTGACTGCAA GCCCGGCTTC 
CAGAGGGATG CATTCGGCAG GACTTGCATT GATGTGAACG AATGCTGGGT CTCGCCGGGC 
CGCCTGTGCC AGCACACATG TGAGAACACA CCGGGCTCCT ACCGCTGCTC CTGCGCTGCT 
GGCTTCCTTT TGGCCGCAGA TGGCAAACAT TGTGAAGATG TGAACGAGTG CGAGACTCGG 
CGCTGCAGCC AGGAATGTGC CAACATCTAT GGCTCCTATC AGTGCTACTG CCGTCAGGGC 
TACCAGCTGG CAGAGGATGG GCATACCTGC ACAGACATCG ATGAGTGTGC ACAGGGCGCG 
GGCATTCTCT GTACCTTCCG CTGTGTCAAC GTGCCTGGGA GCTACCAGTG TGCATGCCCA 
GAGCAAGGGT ATACAATGAT GGCCAACGGG AGGTCCTGCA AGGACCTGGA TGAGTGTGCA 
CTGGGCACCC ACAACTGCTC TGAGGCTGAG ACCTGCCACA ATATCCAGGG GAGTTTCCGC 
TGCCTGCGCT TTGATTGTCC ACCCAACTAT GTCCGTGTCT CAGAAACGAA GTGCGAGCGC 
ACCACATGCC AGGATATCAC GGAATGTCAA ACCTCACCAG CTCGCATCAC GCACTACCAG 
CTCAATTTCC AGACAGGCCT ACTGGTACCT GCACATATCT TCCGCATCGG CCCTGCTCCC 
GCCTTTGCTG GGGACACCAT CTCCCTGACC ATCACGAAGG GCAATGAGGA GGGCTACTTC 
GTCACACGCA GACTCAATGC CTACACTGGT GTGGTATCCC TGCAGCGGTC TGTTCTGGAG 
CCGCGGGACT TTGCCCTAGA TGTGGAGATG AAGCTTTGGC GCCAGGGCTC TGTCACTACC 
TTCCTGGCCA AGATGTACAT CTTCTTCACC ACTTTTGCCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030111016.1
CDS	284..3808
Translation	MLLQESAGVWLALALVTALTPSPSMAVPWQDCTGAECPLLENCIEEALEPGACCATCVQQGCACEGYQYYDCVQGGFVDGRVPAGQSYFVDFGSTECSCPPGGGKISCQFMLCPELPPNCIEAVVVADSCPQCGQVGCVHSGRKYAAGHTVHLSSCRACHCPDAGGELICYQLPGCHGNFSDAEEGDSERQYEDPYSYDQEVAEAEATTAIVNEVQAGAEGPPAALGGGNLPPSSIRVTPWPVALPRPTAAAALGPPAPVQAKARRVTLDTEEDEEEEEEETLVTEPPTAGSPGRLDSLPTRSPARPGFPVQEKEAEAKAGPEENLIPDAQVTPRSVMQEGAAPLPRSGLAALSPSLATDSSSEDPVKPSDHPTLSTLPPDRAQVSPSPETPEEIPQHPQLLPRFRAEEDIDPNSVHSVPRGDLDGSTKDLIETCCAAGQQWAIDNDECQEIPENGAQSDICRIAQRQCCISYLKEKSCVAGVMGAKEGETCGAEDNDTCGVSLYKQCCDCCGLGLRVRAEGQSCESNPNLGYPCNHVMLSCCEGEEPLIVPEVRRPPEPEAAPRRVSEMEMASREALSLGTEAELPNSLPGDDQDECLMLPGELCQHLCINTVGSYRCACFPGFELQGDGRTCRPDRGAPQLDTARESAPRSESAQVSPNTIPLPVPQPNTCKDNGPCRQVCRVVGDTAMCSCFPGYAIMADGVSCEDINECVTDLHTCTRAEHCVNTPGSFQCYKALTCEPGYVLTDGECTDVDECVTGTHNCQAGFSCQNTKGSFYCQARQRCMDGFLQDPEGNCVDINECTSLLEPCRSGFSCINTVGSYTCQRNPLVCGRGYHANEEGSECVDVNECETGVHRCGEGQLCYNLPGSYRCDCKPGFQRDAFGRTCIDVNECWVSPGRLCQHTCENTPGSYRCSCAAGFLLAADGKHCEDVNECETRRCSQECANIYGSYQCYCRQGYQLAEDGHTCTDIDECAQGAGILCTFRCVNVPGSYQCACPEQGYTMMANGRSCKDLDECALGTHNCSEAETCHNIQGSFRCLRFDCPPNYVRVSETKCERTTCQDITECQTSPARITHYQLNFQTGLLVPAHIFRIGPAPAFAGDTISLTITKGNEEGYFVTRRLNAYTGVVSLQRSVLEPRDFALDVEMKLWRQGSVTTFLAKMYIFFTTFAP

Target ORF information:

RefSeq Version	XM_030255156.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus fibulin 2 (Fbln2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030255156.1

ORF Insert Sequence:

ATGCTGCTCC AGGAATCTGC AGGAGTGTGG CTGGCCTTGG CTCTGGTCAC AGCTCTGACT 
CCGAGCCCAA GCATGGCCGT CCCGTGGCAG GATTGCACAG GTGCTGAGTG TCCCCTGCTG 
GAGAACTGCA TCGAGGAGGC GTTGGAGCCA GGAGCCTGCT GTGCTACCTG TGTGCAGCAG 
GGCTGTGCCT GCGAAGGCTA CCAGTACTAC GACTGCGTAC AAGGAGGGTT TGTGGATGGC 
CGTGTACCAG CCGGCCAGTC CTACTTTGTG GACTTTGGCA GCACCGAGTG CTCCTGCCCA 
CCCGGTGGTG GCAAGATCAG CTGCCAGTTC ATGCTGTGCC CTGAGCTACC ACCCAACTGC 
ATTGAAGCTG TAGTAGTAGC CGACAGCTGC CCGCAGTGTG GCCAGGTGGG CTGTGTCCAC 
AGTGGCCGTA AGTATGCTGC TGGCCATACC GTCCACCTCT CATCCTGCCG GGCTTGCCAC 
TGTCCTGACG CTGGTGGTGA GCTCATTTGC TACCAGCTTC CTGGTTGCCA TGGGAACTTC 
TCGGATGCTG AGGAGGGTGA CTCTGAGAGG CAGTATGAAG ACCCCTACAG CTATGACCAG 
GAGGTGGCAG AGGCAGAAGC TACTACGGCC ATTGTGAACG AGGTCCAGGC AGGTGCAGAG 
GGCCCCCCAG CTGCTCTGGG AGGAGGGAAT CTGCCACCGT CCTCCATCAG AGTAACACCC 
TGGCCAGTTG CCCTTCCCAG ACCCACAGCA GCTGCTGCCC TGGGTCCCCC AGCACCTGTG 
CAGGCCAAAG CCAGGAGAGT GACATTGGAC ACTGAAGAGG ATGAGGAAGA AGAAGAGGAG 
GAGACACTAG TCACTGAGCC ACCAACAGCA GGCAGTCCTG GAAGGCTGGA CAGCCTGCCC 
ACAAGATCCC CAGCCAGACC TGGTTTCCCT GTCCAGGAGA AGGAGGCTGA AGCTAAGGCA 
GGGCCTGAGG AAAACCTTAT TCCTGATGCC CAGGTCACTC CTCGAAGTGT CATGCAGGAG 
GGTGCTGCAC CTCTGCCAAG GTCAGGCCTG GCTGCTCTCA GTCCATCTCT GGCCACTGAC 
AGCTCTTCAG AGGACCCAGT AAAGCCCAGC GATCACCCCA CTCTATCTAC ACTGCCACCT 
GACCGAGCCC AGGTCTCCCC CTCCCCAGAG ACACCTGAAG AAATACCCCA GCATCCACAG 
TTGCTACCTC GATTCCGGGC AGAAGAAGAC ATAGACCCCA ACTCAGTACA TTCTGTCCCC 
AGAGGTGATC TTGATGGCTC CACCAAGGAC CTAATCGAGA CCTGTTGTGC AGCGGGCCAG 
CAGTGGGCCA TCGACAATGA TGAGTGCCAG GAGATCCCCG AGAATGGTGC CCAGAGTGAC 
ATCTGTAGGA TAGCCCAGAG GCAATGCTGC ATCTCCTACT TGAAAGAGAA GAGCTGTGTG 
GCTGGGGTCA TGGGAGCCAA GGAGGGTGAG ACCTGTGGAG CAGAGGACAA TGATACCTGC 
GGCGTCTCAC TCTACAAGCA ATGCTGTGAC TGCTGTGGCC TGGGACTCCG AGTGCGGGCT 
GAGGGCCAGT CCTGTGAGTC CAACCCCAAC CTGGGCTATC CCTGCAACCA TGTCATGCTC 
TCCTGCTGTG AAGGTGAAGA GCCCCTCATA GTGCCTGAGG TCCGCCGGCC ACCAGAGCCC 
GAGGCTGCTC CTCGCAGAGT TTCAGAGATG GAGATGGCAA GCCGGGAGGC CCTGTCACTG 
GGCACAGAGG CTGAACTGCC CAATAGCCTG CCGGGAGATG ACCAGGATGA GTGCCTGATG 
CTCCCTGGGG AGCTTTGCCA GCATCTTTGC ATCAACACCG TCGGCTCCTA CCGCTGTGCC 
TGCTTCCCTG GATTCGAGCT GCAGGGTGAT GGCCGTACCT GCCGCCCAGA TAGAGGTGCC 
CCACAGTTGG ACACTGCACG AGAGTCTGCA CCGAGGTCTG AGTCTGCCCA GGTATCTCCC 
AACACCATCC CACTGCCGGT GCCACAGCCC AACACCTGCA AAGACAATGG GCCCTGCCGT 
CAGGTGTGTC GTGTTGTTGG GGACACAGCT ATGTGCTCCT GCTTCCCTGG CTACGCCATC 
ATGGCAGATG GTGTGTCCTG TGAAGACATC AATGAGTGTG TGACAGACCT GCACACGTGC 
ACCCGGGCCG AGCACTGTGT GAACACGCCA GGTTCCTTCC AATGCTACAA GGCGCTCACC 
TGTGAGCCAG GCTATGTCCT CACAGATGGT GAATGCACAG ACGTGGACGA ATGTGTGACG 
GGCACACACA ACTGCCAGGC AGGCTTCTCA TGCCAGAACA CCAAGGGCTC CTTCTACTGC 
CAGGCCCGGC AACGCTGCAT GGATGGCTTC TTGCAGGACC CCGAGGGCAA CTGTGTGGAC 
ATCAACGAAT GCACATCGTT ACTGGAGCCT TGCCGCTCAG GGTTCAGCTG CATCAACACA 
GTGGGCTCCT ACACCTGTCA GAGGAACCCA CTGGTCTGCG GTCGCGGTTA CCATGCTAAC 
GAGGAGGGCT CTGAATGTGT GGATGTGAAT GAGTGTGAGA CAGGTGTGCA TCGCTGTGGC 
GAGGGCCAAC TGTGCTATAA CCTCCCTGGA TCCTACCGCT GTGACTGCAA GCCCGGCTTC 
CAGAGGGATG CATTCGGCAG GACTTGCATT GATGTGAACG AATGCTGGGT CTCGCCGGGC 
CGCCTGTGCC AGCACACATG TGAGAACACA CCGGGCTCCT ACCGCTGCTC CTGCGCTGCT 
GGCTTCCTTT TGGCCGCAGA TGGCAAACAT TGTGAAGATG TGAACGAGTG CGAGACTCGG 
CGCTGCAGCC AGGAATGTGC CAACATCTAT GGCTCCTATC AGTGCTACTG CCGTCAGGGC 
TACCAGCTGG CAGAGGATGG GCATACCTGC ACAGACATCG ATGAGTGTGC ACAGGGCGCG 
GGCATTCTCT GTACCTTCCG CTGTGTCAAC GTGCCTGGGA GCTACCAGTG TGCATGCCCA 
GAGCAAGGGT ATACAATGAT GGCCAACGGG AGGTCCTGCA AGGACCTGGA TGAGTGTGCA 
CTGGGCACCC ACAACTGCTC TGAGGCTGAG ACCTGCCACA ATATCCAGGG GAGTTTCCGC 
TGCCTGCGCT TTGATTGTCC ACCCAACTAT GTCCGTGTCT CAGAAACGAA GTGCGAGCGC 
ACCACATGCC AGGATATCAC GGAATGTCAA ACCTCACCAG CTCGCATCAC GCACTACCAG 
CTCAATTTCC AGACAGGCCT ACTGGTACCT GCACATATCT TCCGCATCGG CCCTGCTCCC 
GCCTTTGCTG GGGACACCAT CTCCCTGACC ATCACGAAGG GCAATGAGGA GGGCTACTTC 
GTCACACGCA GACTCAATGC CTACACTGGT GTGGTATCCC TGCAGCGGTC TGTTCTGGAG 
CCGCGGGACT TTGCCCTAGA TGTGGAGATG AAGCTTTGGC GCCAGGGCTC TGTCACTACC 
TTCCTGGCCA AGATGTACAT CTTCTTCACC ACTTTTGCCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fbln2 ( XM_030255157.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030111017.1 Mus musculus fibulin 2 (Fbln2), transcript variant X3, mRNA.

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CloneID	OMu89126
Clone ID Related Accession (Same CDS sequence)	XM_030255157.1
Accession Version	XM_030255157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	fibulin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCC AGGAATCTGC AGGAGTGTGG CTGGCCTTGG CTCTGGTCAC AGCTCTGACT 
CCGAGCCCAA GCATGGCCGT CCCGTGGCAG GATTGCACAG GTGCTGAGTG TCCCCTGCTG 
GAGAACTGCA TCGAGGAGGC GTTGGAGCCA GGAGCCTGCT GTGCTACCTG TGTGCAGCAG 
GGCTGTGCCT GCGAAGGCTA CCAGTACTAC GACTGCGTAC AAGGAGGGTT TGTGGATGGC 
CGTGTACCAG CCGGCCAGTC CTACTTTGTG GACTTTGGCA GCACCGAGTG CTCCTGCCCA 
CCCGGTGGTG GCAAGATCAG CTGCCAGTTC ATGCTGTGCC CTGAGCTACC ACCCAACTGC 
ATTGAAGCTG TAGTAGTAGC CGACAGCTGC CCGCAGTGTG GCCAGGTGGG CTGTGTCCAC 
AGTGGCCGTA AGTATGCTGC TGGCCATACC GTCCACCTCT CATCCTGCCG GGCTTGCCAC 
TGTCCTGACG CTGGTGGTGA GCTCATTTGC TACCAGCTTC CTGGTTGCCA TGGGAACTTC 
TCGGATGCTG AGGAGGGTGA CTCTGAGAGG CAGTATGAAG ACCCCTACAG CTATGACCAG 
GAGGTGGCAG AGGCAGAAGC TACTACGGCC ATTGTGAACG AGGTCCAGGC AGGTGCAGAG 
GGCCCCCCAG CTGCTCTGGG AGGAGGGAAT CTGCCACCGT CCTCCATCAG AGTAACACCC 
TGGCCAGTTG CCCTTCCCAG ACCCACAGCA GCTGCTGCCC TGGGTCCCCC AGCACCTGTG 
CAGGCCAAAG CCAGGAGAGT GACATTGGAC ACTGAAGAGG ATGAGGAAGA AGAAGAGGAG 
GAGACACTAG TCACTGAGCC ACCAACAGCA GGCAGTCCTG GAAGGCTGGA CAGCCTGCCC 
ACAAGATCCC CAGCCAGACC TGGTTTCCCT GTCCAGGAGA AGGAGGCTGA AGCTAAGGCA 
GGGCCTGAGG AAAACCTTAT TCCTGATGCC CAGGTCACTC CTCGAAGTGT CATGCAGGAG 
GGTGCTGCAC CTCTGCCAAG GTCAGGCCTG GCTGCTCTCA GTCCATCTCT GGCCACTGAC 
AGCTCTTCAG AGGACCCAGT AAAGCCCAGC GATCACCCCA CTCTATCTAC ACTGCCACCT 
GACCGAGCCC AGGTCTCCCC CTCCCCAGAG ACACCTGAAG AAATACCCCA GCATCCACAG 
TTGCTACCTC GATTCCGGGC AGAAGAAGAC ATAGACCCCA ACTCAGTACA TTCTGTCCCC 
AGAGGTGATC TTGATGGCTC CACCAAGGAC CTAATCGAGA CCTGTTGTGC AGCGGGCCAG 
CAGTGGGCCA TCGACAATGA TGAGTGCCAG GAGATCCCCG AGAATGGTGC CCAGAGTGAC 
ATCTGTAGGA TAGCCCAGAG GCAATGCTGC ATCTCCTACT TGAAAGAGAA GAGCTGTGTG 
GCTGGGGTCA TGGGAGCCAA GGAGGGTGAG ACCTGTGGAG CAGAGGACAA TGATACCTGC 
GGCGTCTCAC TCTACAAGCA ATGCTGTGAC TGCTGTGGCC TGGGACTCCG AGTGCGGGCT 
GAGGGCCAGT CCTGTGAGTC CAACCCCAAC CTGGGCTATC CCTGCAACCA TGTCATGCTC 
TCCTGCTGTG AAGGTGAAGA GCCCCTCATA GTGCCTGAGG TCCGCCGGCC ACCAGAGCCC 
GAGGCTGCTC CTCGCAGAGT TTCAGAGATG GAGATGGCAA GCCGGGAGGC CCTGTCACTG 
GGCACAGAGG CTGAACTGCC CAATAGCCTG CCGGGAGATG ACCAGGATGA GTGCCTGATG 
CTCCCTGGGG AGCTTTGCCA GCATCTTTGC ATCAACACCG TCGGCTCCTA CCGCTGTGCC 
TGCTTCCCTG GATTCGAGCT GCAGGGTGAT GGCCGTACCT GCCGCCCAGA TAGAGGTGCC 
CCACAGTTGG ACACTGCACG AGAGTCTGCA CCGAGGTCTG AGTCTGCCCA GGTATCTCCC 
AACACCATCC CACTGCCGGT GCCACAGCCC AACACCTGCA AAGACAATGG GCCCTGCCGT 
CAGGTGTGTC GTGTTGTTGG GGACACAGCT ATGTGCTCCT GCTTCCCTGG CTACGCCATC 
ATGGCAGATG GTGTGTCCTG TGAAGACCAA GACGAGTGTT TGATGGGCAC TCACGATTGT 
AGCTGGAAAC AGTTCTGTGT GAACACCCTG GGATCCTTCT ACTGTGTCAA CCACACAGTG 
CTGTGTGCAG AGGGCTATAT CCTCAACGCA CATAGGAAGT GTGTGGACAT CAATGAGTGT 
GTGACAGACC TGCACACGTG CACCCGGGCC GAGCACTGTG TGAACACGCC AGGTTCCTTC 
CAATGCTACA AGGCGCTCAC CTGTGAGCCA GGCTATGTCC TCACAGATGG TGAATGCACA 
GACGTGGACG AATGTGTGAC GGGCACACAC AACTGCCAGG CAGGCTTCTC ATGCCAGAAC 
ACCAAGGGCT CCTTCTACTG CCAGGCCCGG CAACGCTGCA TGGATGGCTT CTTGCAGGAC 
CCCGAGGGCA ACTGTGTGGA CATCAACGAA TGCACATCGT TACTGGAGCC TTGCCGCTCA 
GGGTTCAGCT GCATCAACAC AGTGGGCTCC TACACCTGTC AGAGGAACCC ACTGGTCTGC 
GGTCGCGGTT ACCATGCTAA CGAGGAGGGC TCTGAATGTG TGGATGTGAA TGAGTGTGAG 
ACAGGTGTGC ATCGCTGTGG CGAGGGCCAA CTGTGCTATA ACCTCCCTGG ATCCTACCGC 
TGTGACTGCA AGCCCGGCTT CCAGAGGGAT GCATTCGGCA GGACTTGCAT TGATGTGAAC 
GAATGCTGGG TCTCGCCGGG CCGCCTGTGC CAGCACACAT GTGAGAACAC ACCGGGCTCC 
TACCGCTGCT CCTGCGCTGC TGGCTTCCTT TTGGCCGCAG ATGGCAAACA TTGTGAAGAT 
GTGAACGAGT GCGAGACTCG GCGCTGCAGC CAGGAATGTG CCAACATCTA TGGCTCCTAT 
CAGTGCTACT GCCGTCAGGG CTACCAGCTG GCAGAGGATG GGCATACCTG CACAGACATC 
GATGAGTGTG CACAGGGCGC GGGCATTCTC TGTACCTTCC GCTGTGTCAA CGTGCCTGGG 
AGCTACCAGT GTGCATGCCC AGAGCAAGGG TATACAATGA TGGCCAACGG GAGGTCCTGC 
AAGGACCTGG ATGAGTGTGC ACTGGGCACC CACAACTGCT CTGAGGCTGA GACCTGCCAC 
AATATCCAGG GGAGTTTCCG CTGCCTGCGC TTTGATTGTC CACCCAACTA TGTCCGTGTC 
TCAGAAACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030111017.1
CDS	455..3766
Translation	MLLQESAGVWLALALVTALTPSPSMAVPWQDCTGAECPLLENCIEEALEPGACCATCVQQGCACEGYQYYDCVQGGFVDGRVPAGQSYFVDFGSTECSCPPGGGKISCQFMLCPELPPNCIEAVVVADSCPQCGQVGCVHSGRKYAAGHTVHLSSCRACHCPDAGGELICYQLPGCHGNFSDAEEGDSERQYEDPYSYDQEVAEAEATTAIVNEVQAGAEGPPAALGGGNLPPSSIRVTPWPVALPRPTAAAALGPPAPVQAKARRVTLDTEEDEEEEEEETLVTEPPTAGSPGRLDSLPTRSPARPGFPVQEKEAEAKAGPEENLIPDAQVTPRSVMQEGAAPLPRSGLAALSPSLATDSSSEDPVKPSDHPTLSTLPPDRAQVSPSPETPEEIPQHPQLLPRFRAEEDIDPNSVHSVPRGDLDGSTKDLIETCCAAGQQWAIDNDECQEIPENGAQSDICRIAQRQCCISYLKEKSCVAGVMGAKEGETCGAEDNDTCGVSLYKQCCDCCGLGLRVRAEGQSCESNPNLGYPCNHVMLSCCEGEEPLIVPEVRRPPEPEAAPRRVSEMEMASREALSLGTEAELPNSLPGDDQDECLMLPGELCQHLCINTVGSYRCACFPGFELQGDGRTCRPDRGAPQLDTARESAPRSESAQVSPNTIPLPVPQPNTCKDNGPCRQVCRVVGDTAMCSCFPGYAIMADGVSCEDQDECLMGTHDCSWKQFCVNTLGSFYCVNHTVLCAEGYILNAHRKCVDINECVTDLHTCTRAEHCVNTPGSFQCYKALTCEPGYVLTDGECTDVDECVTGTHNCQAGFSCQNTKGSFYCQARQRCMDGFLQDPEGNCVDINECTSLLEPCRSGFSCINTVGSYTCQRNPLVCGRGYHANEEGSECVDVNECETGVHRCGEGQLCYNLPGSYRCDCKPGFQRDAFGRTCIDVNECWVSPGRLCQHTCENTPGSYRCSCAAGFLLAADGKHCEDVNECETRRCSQECANIYGSYQCYCRQGYQLAEDGHTCTDIDECAQGAGILCTFRCVNVPGSYQCACPEQGYTMMANGRSCKDLDECALGTHNCSEAETCHNIQGSFRCLRFDCPPNYVRVSET

Target ORF information:

RefSeq Version	XM_030255157.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus fibulin 2 (Fbln2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030255157.1

ORF Insert Sequence:

ATGCTGCTCC AGGAATCTGC AGGAGTGTGG CTGGCCTTGG CTCTGGTCAC AGCTCTGACT 
CCGAGCCCAA GCATGGCCGT CCCGTGGCAG GATTGCACAG GTGCTGAGTG TCCCCTGCTG 
GAGAACTGCA TCGAGGAGGC GTTGGAGCCA GGAGCCTGCT GTGCTACCTG TGTGCAGCAG 
GGCTGTGCCT GCGAAGGCTA CCAGTACTAC GACTGCGTAC AAGGAGGGTT TGTGGATGGC 
CGTGTACCAG CCGGCCAGTC CTACTTTGTG GACTTTGGCA GCACCGAGTG CTCCTGCCCA 
CCCGGTGGTG GCAAGATCAG CTGCCAGTTC ATGCTGTGCC CTGAGCTACC ACCCAACTGC 
ATTGAAGCTG TAGTAGTAGC CGACAGCTGC CCGCAGTGTG GCCAGGTGGG CTGTGTCCAC 
AGTGGCCGTA AGTATGCTGC TGGCCATACC GTCCACCTCT CATCCTGCCG GGCTTGCCAC 
TGTCCTGACG CTGGTGGTGA GCTCATTTGC TACCAGCTTC CTGGTTGCCA TGGGAACTTC 
TCGGATGCTG AGGAGGGTGA CTCTGAGAGG CAGTATGAAG ACCCCTACAG CTATGACCAG 
GAGGTGGCAG AGGCAGAAGC TACTACGGCC ATTGTGAACG AGGTCCAGGC AGGTGCAGAG 
GGCCCCCCAG CTGCTCTGGG AGGAGGGAAT CTGCCACCGT CCTCCATCAG AGTAACACCC 
TGGCCAGTTG CCCTTCCCAG ACCCACAGCA GCTGCTGCCC TGGGTCCCCC AGCACCTGTG 
CAGGCCAAAG CCAGGAGAGT GACATTGGAC ACTGAAGAGG ATGAGGAAGA AGAAGAGGAG 
GAGACACTAG TCACTGAGCC ACCAACAGCA GGCAGTCCTG GAAGGCTGGA CAGCCTGCCC 
ACAAGATCCC CAGCCAGACC TGGTTTCCCT GTCCAGGAGA AGGAGGCTGA AGCTAAGGCA 
GGGCCTGAGG AAAACCTTAT TCCTGATGCC CAGGTCACTC CTCGAAGTGT CATGCAGGAG 
GGTGCTGCAC CTCTGCCAAG GTCAGGCCTG GCTGCTCTCA GTCCATCTCT GGCCACTGAC 
AGCTCTTCAG AGGACCCAGT AAAGCCCAGC GATCACCCCA CTCTATCTAC ACTGCCACCT 
GACCGAGCCC AGGTCTCCCC CTCCCCAGAG ACACCTGAAG AAATACCCCA GCATCCACAG 
TTGCTACCTC GATTCCGGGC AGAAGAAGAC ATAGACCCCA ACTCAGTACA TTCTGTCCCC 
AGAGGTGATC TTGATGGCTC CACCAAGGAC CTAATCGAGA CCTGTTGTGC AGCGGGCCAG 
CAGTGGGCCA TCGACAATGA TGAGTGCCAG GAGATCCCCG AGAATGGTGC CCAGAGTGAC 
ATCTGTAGGA TAGCCCAGAG GCAATGCTGC ATCTCCTACT TGAAAGAGAA GAGCTGTGTG 
GCTGGGGTCA TGGGAGCCAA GGAGGGTGAG ACCTGTGGAG CAGAGGACAA TGATACCTGC 
GGCGTCTCAC TCTACAAGCA ATGCTGTGAC TGCTGTGGCC TGGGACTCCG AGTGCGGGCT 
GAGGGCCAGT CCTGTGAGTC CAACCCCAAC CTGGGCTATC CCTGCAACCA TGTCATGCTC 
TCCTGCTGTG AAGGTGAAGA GCCCCTCATA GTGCCTGAGG TCCGCCGGCC ACCAGAGCCC 
GAGGCTGCTC CTCGCAGAGT TTCAGAGATG GAGATGGCAA GCCGGGAGGC CCTGTCACTG 
GGCACAGAGG CTGAACTGCC CAATAGCCTG CCGGGAGATG ACCAGGATGA GTGCCTGATG 
CTCCCTGGGG AGCTTTGCCA GCATCTTTGC ATCAACACCG TCGGCTCCTA CCGCTGTGCC 
TGCTTCCCTG GATTCGAGCT GCAGGGTGAT GGCCGTACCT GCCGCCCAGA TAGAGGTGCC 
CCACAGTTGG ACACTGCACG AGAGTCTGCA CCGAGGTCTG AGTCTGCCCA GGTATCTCCC 
AACACCATCC CACTGCCGGT GCCACAGCCC AACACCTGCA AAGACAATGG GCCCTGCCGT 
CAGGTGTGTC GTGTTGTTGG GGACACAGCT ATGTGCTCCT GCTTCCCTGG CTACGCCATC 
ATGGCAGATG GTGTGTCCTG TGAAGACCAA GACGAGTGTT TGATGGGCAC TCACGATTGT 
AGCTGGAAAC AGTTCTGTGT GAACACCCTG GGATCCTTCT ACTGTGTCAA CCACACAGTG 
CTGTGTGCAG AGGGCTATAT CCTCAACGCA CATAGGAAGT GTGTGGACAT CAATGAGTGT 
GTGACAGACC TGCACACGTG CACCCGGGCC GAGCACTGTG TGAACACGCC AGGTTCCTTC 
CAATGCTACA AGGCGCTCAC CTGTGAGCCA GGCTATGTCC TCACAGATGG TGAATGCACA 
GACGTGGACG AATGTGTGAC GGGCACACAC AACTGCCAGG CAGGCTTCTC ATGCCAGAAC 
ACCAAGGGCT CCTTCTACTG CCAGGCCCGG CAACGCTGCA TGGATGGCTT CTTGCAGGAC 
CCCGAGGGCA ACTGTGTGGA CATCAACGAA TGCACATCGT TACTGGAGCC TTGCCGCTCA 
GGGTTCAGCT GCATCAACAC AGTGGGCTCC TACACCTGTC AGAGGAACCC ACTGGTCTGC 
GGTCGCGGTT ACCATGCTAA CGAGGAGGGC TCTGAATGTG TGGATGTGAA TGAGTGTGAG 
ACAGGTGTGC ATCGCTGTGG CGAGGGCCAA CTGTGCTATA ACCTCCCTGG ATCCTACCGC 
TGTGACTGCA AGCCCGGCTT CCAGAGGGAT GCATTCGGCA GGACTTGCAT TGATGTGAAC 
GAATGCTGGG TCTCGCCGGG CCGCCTGTGC CAGCACACAT GTGAGAACAC ACCGGGCTCC 
TACCGCTGCT CCTGCGCTGC TGGCTTCCTT TTGGCCGCAG ATGGCAAACA TTGTGAAGAT 
GTGAACGAGT GCGAGACTCG GCGCTGCAGC CAGGAATGTG CCAACATCTA TGGCTCCTAT 
CAGTGCTACT GCCGTCAGGG CTACCAGCTG GCAGAGGATG GGCATACCTG CACAGACATC 
GATGAGTGTG CACAGGGCGC GGGCATTCTC TGTACCTTCC GCTGTGTCAA CGTGCCTGGG 
AGCTACCAGT GTGCATGCCC AGAGCAAGGG TATACAATGA TGGCCAACGG GAGGTCCTGC 
AAGGACCTGG ATGAGTGTGC ACTGGGCACC CACAACTGCT CTGAGGCTGA GACCTGCCAC 
AATATCCAGG GGAGTTTCCG CTGCCTGCGC TTTGATTGTC CACCCAACTA TGTCCGTGTC 
TCAGAAACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fbln2 ( NM_007992.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032018.2 Mus musculus fibulin 2 (Fbln2), transcript variant 1, mRNA.

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CloneID	OMu01192
Clone ID Related Accession (Same CDS sequence)	XM_006505530.4 , NM_007992.2 , XM_006505530.3
Accession Version	NM_007992.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	fibulin-2 isoform a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC131764.3 and AC121990.3. On Feb 7, 2007 this sequence version replaced NM_007992.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X75285.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTCC AGGAATCTGC AGGAGTGTGG CTGGCCTTGG CTCTGGTCAC AGCTCTGACT 
CCGAGCCCAA GCATGGCCGT CCCGTGGCAG GATTGCACAG GTGCTGAGTG TCCCCTGCTG 
GAGAACTGCA TCGAGGAGGC GTTGGAGCCA GGAGCCTGCT GTGCTACCTG TGTGCAGCAG 
GGCTGTGCCT GCGAAGGCTA CCAGTACTAC GACTGCGTAC AAGGAGGGTT TGTGGATGGC 
CGTGTACCAG CCGGCCAGTC CTACTTTGTG GACTTTGGCA GCACCGAGTG CTCCTGCCCA 
CCCGGTGGTG GCAAGATCAG CTGCCAGTTC ATGCTGTGCC CTGAGCTACC ACCCAACTGC 
ATTGAAGCTG TAGTAGTAGC CGACAGCTGC CCGCAGTGTG GCCAGGTGGG CTGTGTCCAC 
AGTGGCCGTA AGTATGCTGC TGGCCATACC GTCCACCTCT CATCCTGCCG GGCTTGCCAC 
TGTCCTGACG CTGGTGGTGA GCTCATTTGC TACCAGCTTC CTGGTTGCCA TGGGAACTTC 
TCGGATGCTG AGGAGGGTGA CTCTGAGAGG CAGTATGAAG ACCCCTACAG CTATGACCAG 
GAGGTGGCAG AGGCAGAAGC TACTACGGCC ATTGTGAACG AGGTCCAGGC AGGTGCAGAG 
GGCCCCCCAG CTGCTCTGGG AGGAGGGAAT CTGCCACCGT CCTCCATCAG AGTAACACCC 
TGGCCAGTTG CCCTTCCCAG ACCCACAGCA GCTGCTGCCC TGGGTCCCCC AGCACCTGTG 
CAGGCCAAAG CCAGGAGAGT GACATTGGAC ACTGAAGAGG ATGAGGAAGA AGAAGAGGAG 
GAGACACTAG TCACTGAGCC ACCAACAGCA GGCAGTCCTG GAAGGCTGGA CAGCCTGCCC 
ACAAGATCCC CAGCCAGACC TGGTTTCCCT GTCCAGGAGA AGGAGGCTGA AGCTAAGGCA 
GGGCCTGAGG AAAACCTTAT TCCTGATGCC CAGGTCACTC CTCGAAGTGT CATGCAGGAG 
GGTGCTGCAC CTCTGCCAAG GTCAGGCCTG GCTGCTCTCA GTCCATCTCT GGCCACTGAC 
AGCTCTTCAG AGGACCCAGT AAAGCCCAGC GATCACCCCA CTCTATCTAC ACTGCCACCT 
GACCGAGCCC AGGTCTCCCC CTCCCCAGAG ACACCTGAAG AAATACCCCA GCATCCACAG 
TTGCTACCTC GATTCCGGGC AGAAGAAGAC ATAGACCCCA ACTCAGTACA TTCTGTCCCC 
AGAGGTGATC TTGATGGCTC CACCAAGGAC CTAATCGAGA CCTGTTGTGC AGCGGGCCAG 
CAGTGGGCCA TCGACAATGA TGAGTGCCAG GAGATCCCCG AGAATGGTGC CCAGAGTGAC 
ATCTGTAGGA TAGCCCAGAG GCAATGCTGC ATCTCCTACT TGAAAGAGAA GAGCTGTGTG 
GCTGGGGTCA TGGGAGCCAA GGAGGGTGAG ACCTGTGGAG CAGAGGACAA TGATACCTGC 
GGCGTCTCAC TCTACAAGCA ATGCTGTGAC TGCTGTGGCC TGGGACTCCG AGTGCGGGCT 
GAGGGCCAGT CCTGTGAGTC CAACCCCAAC CTGGGCTATC CCTGCAACCA TGTCATGCTC 
TCCTGCTGTG AAGGTGAAGA GCCCCTCATA GTGCCTGAGG TCCGCCGGCC ACCAGAGCCC 
GAGGCTGCTC CTCGCAGAGT TTCAGAGATG GAGATGGCAA GCCGGGAGGC CCTGTCACTG 
GGCACAGAGG CTGAACTGCC CAATAGCCTG CCGGGAGATG ACCAGGATGA GTGCCTGATG 
CTCCCTGGGG AGCTTTGCCA GCATCTTTGC ATCAACACCG TCGGCTCCTA CCGCTGTGCC 
TGCTTCCCTG GATTCGAGCT GCAGGGTGAT GGCCGTACCT GCCGCCCAGA TAGAGGTGCC 
CCACAGTTGG ACACTGCACG AGAGTCTGCA CCGAGGTCTG AGTCTGCCCA GGTATCTCCC 
AACACCATCC CACTGCCGGT GCCACAGCCC AACACCTGCA AAGACAATGG GCCCTGCCGT 
CAGGTGTGTC GTGTTGTTGG GGACACAGCT ATGTGCTCCT GCTTCCCTGG CTACGCCATC 
ATGGCAGATG GTGTGTCCTG TGAAGACCAA GACGAGTGTT TGATGGGCAC TCACGATTGT 
AGCTGGAAAC AGTTCTGTGT GAACACCCTG GGATCCTTCT ACTGTGTCAA CCACACAGTG 
CTGTGTGCAG AGGGCTATAT CCTCAACGCA CATAGGAAGT GTGTGGACAT CAATGAGTGT 
GTGACAGACC TGCACACGTG CACCCGGGCC GAGCACTGTG TGAACACGCC AGGTTCCTTC 
CAATGCTACA AGGCGCTCAC CTGTGAGCCA GGCTATGTCC TCACAGATGG TGAATGCACA 
GACGTGGACG AATGTGTGAC GGGCACACAC AACTGCCAGG CAGGCTTCTC ATGCCAGAAC 
ACCAAGGGCT CCTTCTACTG CCAGGCCCGG CAACGCTGCA TGGATGGCTT CTTGCAGGAC 
CCCGAGGGCA ACTGTGTGGA CATCAACGAA TGCACATCGT TACTGGAGCC TTGCCGCTCA 
GGGTTCAGCT GCATCAACAC AGTGGGCTCC TACACCTGTC AGAGGAACCC ACTGGTCTGC 
GGTCGCGGTT ACCATGCTAA CGAGGAGGGC TCTGAATGTG TGGATGTGAA TGAGTGTGAG 
ACAGGTGTGC ATCGCTGTGG CGAGGGCCAA CTGTGCTATA ACCTCCCTGG ATCCTACCGC 
TGTGACTGCA AGCCCGGCTT CCAGAGGGAT GCATTCGGCA GGACTTGCAT TGATGTGAAC 
GAATGCTGGG TCTCGCCGGG CCGCCTGTGC CAGCACACAT GTGAGAACAC ACCGGGCTCC 
TACCGCTGCT CCTGCGCTGC TGGCTTCCTT TTGGCCGCAG ATGGCAAACA TTGTGAAGAT 
GTGAACGAGT GCGAGACTCG GCGCTGCAGC CAGGAATGTG CCAACATCTA TGGCTCCTAT 
CAGTGCTACT GCCGTCAGGG CTACCAGCTG GCAGAGGATG GGCATACCTG CACAGACATC 
GATGAGTGTG CACAGGGCGC GGGCATTCTC TGTACCTTCC GCTGTGTCAA CGTGCCTGGG 
AGCTACCAGT GTGCATGCCC AGAGCAAGGG TATACAATGA TGGCCAACGG GAGGTCCTGC 
AAGGACCTGG ATGAGTGTGC ACTGGGCACC CACAACTGCT CTGAGGCTGA GACCTGCCAC 
AATATCCAGG GGAGTTTCCG CTGCCTGCGC TTTGATTGTC CACCCAACTA TGTCCGTGTC 
TCAGAAACGA AGTGCGAGCG CACCACATGC CAGGATATCA CGGAATGTCA AACCTCACCA 
GCTCGCATCA CGCACTACCA GCTCAATTTC CAGACAGGCC TACTGGTACC TGCACATATC 
TTCCGCATCG GCCCTGCTCC CGCCTTTGCT GGGGACACCA TCTCCCTGAC CATCACGAAG 
GGCAATGAGG AGGGCTACTT CGTCACACGC AGACTCAATG CCTACACTGG TGTGGTATCC 
CTGCAGCGGT CTGTTCTGGA GCCGCGGGAC TTTGCCCTAG ATGTGGAGAT GAAGCTTTGG 
CGCCAGGGCT CTGTCACTAC CTTCCTGGCC AAGATGTACA TCTTCTTCAC CACTTTTGCC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032018.2
CDS	151..3816
Translation	MLLQESAGVWLALALVTALTPSPSMAVPWQDCTGAECPLLENCIEEALEPGACCATCVQQGCACEGYQYYDCVQGGFVDGRVPAGQSYFVDFGSTECSCPPGGGKISCQFMLCPELPPNCIEAVVVADSCPQCGQVGCVHSGRKYAAGHTVHLSSCRACHCPDAGGELICYQLPGCHGNFSDAEEGDSERQYEDPYSYDQEVAEAEATTAIVNEVQAGAEGPPAALGGGNLPPSSIRVTPWPVALPRPTAAAALGPPAPVQAKARRVTLDTEEDEEEEEEETLVTEPPTAGSPGRLDSLPTRSPARPGFPVQEKEAEAKAGPEENLIPDAQVTPRSVMQEGAAPLPRSGLAALSPSLATDSSSEDPVKPSDHPTLSTLPPDRAQVSPSPETPEEIPQHPQLLPRFRAEEDIDPNSVHSVPRGDLDGSTKDLIETCCAAGQQWAIDNDECQEIPENGAQSDICRIAQRQCCISYLKEKSCVAGVMGAKEGETCGAEDNDTCGVSLYKQCCDCCGLGLRVRAEGQSCESNPNLGYPCNHVMLSCCEGEEPLIVPEVRRPPEPEAAPRRVSEMEMASREALSLGTEAELPNSLPGDDQDECLMLPGELCQHLCINTVGSYRCACFPGFELQGDGRTCRPDRGAPQLDTARESAPRSESAQVSPNTIPLPVPQPNTCKDNGPCRQVCRVVGDTAMCSCFPGYAIMADGVSCEDQDECLMGTHDCSWKQFCVNTLGSFYCVNHTVLCAEGYILNAHRKCVDINECVTDLHTCTRAEHCVNTPGSFQCYKALTCEPGYVLTDGECTDVDECVTGTHNCQAGFSCQNTKGSFYCQARQRCMDGFLQDPEGNCVDINECTSLLEPCRSGFSCINTVGSYTCQRNPLVCGRGYHANEEGSECVDVNECETGVHRCGEGQLCYNLPGSYRCDCKPGFQRDAFGRTCIDVNECWVSPGRLCQHTCENTPGSYRCSCAAGFLLAADGKHCEDVNECETRRCSQECANIYGSYQCYCRQGYQLAEDGHTCTDIDECAQGAGILCTFRCVNVPGSYQCACPEQGYTMMANGRSCKDLDECALGTHNCSEAETCHNIQGSFRCLRFDCPPNYVRVSETKCERTTCQDITECQTSPARITHYQLNFQTGLLVPAHIFRIGPAPAFAGDTISLTITKGNEEGYFVTRRLNAYTGVVSLQRSVLEPRDFALDVEMKLWRQGSVTTFLAKMYIFFTTFAP

Target ORF information:

RefSeq Version	NM_007992.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus fibulin 2 (Fbln2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007992.2

ORF Insert Sequence:

ATGCTGCTCC AGGAATCTGC AGGAGTGTGG CTGGCCTTGG CTCTGGTCAC AGCTCTGACT 
CCGAGCCCAA GCATGGCCGT CCCGTGGCAG GATTGCACAG GTGCTGAGTG TCCCCTGCTG 
GAGAACTGCA TCGAGGAGGC GTTGGAGCCA GGAGCCTGCT GTGCTACCTG TGTGCAGCAG 
GGCTGTGCCT GCGAAGGCTA CCAGTACTAC GACTGCGTAC AAGGAGGGTT TGTGGATGGC 
CGTGTACCAG CCGGCCAGTC CTACTTTGTG GACTTTGGCA GCACCGAGTG CTCCTGCCCA 
CCCGGTGGTG GCAAGATCAG CTGCCAGTTC ATGCTGTGCC CTGAGCTACC ACCCAACTGC 
ATTGAAGCTG TAGTAGTAGC CGACAGCTGC CCGCAGTGTG GCCAGGTGGG CTGTGTCCAC 
AGTGGCCGTA AGTATGCTGC TGGCCATACC GTCCACCTCT CATCCTGCCG GGCTTGCCAC 
TGTCCTGACG CTGGTGGTGA GCTCATTTGC TACCAGCTTC CTGGTTGCCA TGGGAACTTC 
TCGGATGCTG AGGAGGGTGA CTCTGAGAGG CAGTATGAAG ACCCCTACAG CTATGACCAG 
GAGGTGGCAG AGGCAGAAGC TACTACGGCC ATTGTGAACG AGGTCCAGGC AGGTGCAGAG 
GGCCCCCCAG CTGCTCTGGG AGGAGGGAAT CTGCCACCGT CCTCCATCAG AGTAACACCC 
TGGCCAGTTG CCCTTCCCAG ACCCACAGCA GCTGCTGCCC TGGGTCCCCC AGCACCTGTG 
CAGGCCAAAG CCAGGAGAGT GACATTGGAC ACTGAAGAGG ATGAGGAAGA AGAAGAGGAG 
GAGACACTAG TCACTGAGCC ACCAACAGCA GGCAGTCCTG GAAGGCTGGA CAGCCTGCCC 
ACAAGATCCC CAGCCAGACC TGGTTTCCCT GTCCAGGAGA AGGAGGCTGA AGCTAAGGCA 
GGGCCTGAGG AAAACCTTAT TCCTGATGCC CAGGTCACTC CTCGAAGTGT CATGCAGGAG 
GGTGCTGCAC CTCTGCCAAG GTCAGGCCTG GCTGCTCTCA GTCCATCTCT GGCCACTGAC 
AGCTCTTCAG AGGACCCAGT AAAGCCCAGC GATCACCCCA CTCTATCTAC ACTGCCACCT 
GACCGAGCCC AGGTCTCCCC CTCCCCAGAG ACACCTGAAG AAATACCCCA GCATCCACAG 
TTGCTACCTC GATTCCGGGC AGAAGAAGAC ATAGACCCCA ACTCAGTACA TTCTGTCCCC 
AGAGGTGATC TTGATGGCTC CACCAAGGAC CTAATCGAGA CCTGTTGTGC AGCGGGCCAG 
CAGTGGGCCA TCGACAATGA TGAGTGCCAG GAGATCCCCG AGAATGGTGC CCAGAGTGAC 
ATCTGTAGGA TAGCCCAGAG GCAATGCTGC ATCTCCTACT TGAAAGAGAA GAGCTGTGTG 
GCTGGGGTCA TGGGAGCCAA GGAGGGTGAG ACCTGTGGAG CAGAGGACAA TGATACCTGC 
GGCGTCTCAC TCTACAAGCA ATGCTGTGAC TGCTGTGGCC TGGGACTCCG AGTGCGGGCT 
GAGGGCCAGT CCTGTGAGTC CAACCCCAAC CTGGGCTATC CCTGCAACCA TGTCATGCTC 
TCCTGCTGTG AAGGTGAAGA GCCCCTCATA GTGCCTGAGG TCCGCCGGCC ACCAGAGCCC 
GAGGCTGCTC CTCGCAGAGT TTCAGAGATG GAGATGGCAA GCCGGGAGGC CCTGTCACTG 
GGCACAGAGG CTGAACTGCC CAATAGCCTG CCGGGAGATG ACCAGGATGA GTGCCTGATG 
CTCCCTGGGG AGCTTTGCCA GCATCTTTGC ATCAACACCG TCGGCTCCTA CCGCTGTGCC 
TGCTTCCCTG GATTCGAGCT GCAGGGTGAT GGCCGTACCT GCCGCCCAGA TAGAGGTGCC 
CCACAGTTGG ACACTGCACG AGAGTCTGCA CCGAGGTCTG AGTCTGCCCA GGTATCTCCC 
AACACCATCC CACTGCCGGT GCCACAGCCC AACACCTGCA AAGACAATGG GCCCTGCCGT 
CAGGTGTGTC GTGTTGTTGG GGACACAGCT ATGTGCTCCT GCTTCCCTGG CTACGCCATC 
ATGGCAGATG GTGTGTCCTG TGAAGACCAA GACGAGTGTT TGATGGGCAC TCACGATTGT 
AGCTGGAAAC AGTTCTGTGT GAACACCCTG GGATCCTTCT ACTGTGTCAA CCACACAGTG 
CTGTGTGCAG AGGGCTATAT CCTCAACGCA CATAGGAAGT GTGTGGACAT CAATGAGTGT 
GTGACAGACC TGCACACGTG CACCCGGGCC GAGCACTGTG TGAACACGCC AGGTTCCTTC 
CAATGCTACA AGGCGCTCAC CTGTGAGCCA GGCTATGTCC TCACAGATGG TGAATGCACA 
GACGTGGACG AATGTGTGAC GGGCACACAC AACTGCCAGG CAGGCTTCTC ATGCCAGAAC 
ACCAAGGGCT CCTTCTACTG CCAGGCCCGG CAACGCTGCA TGGATGGCTT CTTGCAGGAC 
CCCGAGGGCA ACTGTGTGGA CATCAACGAA TGCACATCGT TACTGGAGCC TTGCCGCTCA 
GGGTTCAGCT GCATCAACAC AGTGGGCTCC TACACCTGTC AGAGGAACCC ACTGGTCTGC 
GGTCGCGGTT ACCATGCTAA CGAGGAGGGC TCTGAATGTG TGGATGTGAA TGAGTGTGAG 
ACAGGTGTGC ATCGCTGTGG CGAGGGCCAA CTGTGCTATA ACCTCCCTGG ATCCTACCGC 
TGTGACTGCA AGCCCGGCTT CCAGAGGGAT GCATTCGGCA GGACTTGCAT TGATGTGAAC 
GAATGCTGGG TCTCGCCGGG CCGCCTGTGC CAGCACACAT GTGAGAACAC ACCGGGCTCC 
TACCGCTGCT CCTGCGCTGC TGGCTTCCTT TTGGCCGCAG ATGGCAAACA TTGTGAAGAT 
GTGAACGAGT GCGAGACTCG GCGCTGCAGC CAGGAATGTG CCAACATCTA TGGCTCCTAT 
CAGTGCTACT GCCGTCAGGG CTACCAGCTG GCAGAGGATG GGCATACCTG CACAGACATC 
GATGAGTGTG CACAGGGCGC GGGCATTCTC TGTACCTTCC GCTGTGTCAA CGTGCCTGGG 
AGCTACCAGT GTGCATGCCC AGAGCAAGGG TATACAATGA TGGCCAACGG GAGGTCCTGC 
AAGGACCTGG ATGAGTGTGC ACTGGGCACC CACAACTGCT CTGAGGCTGA GACCTGCCAC 
AATATCCAGG GGAGTTTCCG CTGCCTGCGC TTTGATTGTC CACCCAACTA TGTCCGTGTC 
TCAGAAACGA AGTGCGAGCG CACCACATGC CAGGATATCA CGGAATGTCA AACCTCACCA 
GCTCGCATCA CGCACTACCA GCTCAATTTC CAGACAGGCC TACTGGTACC TGCACATATC 
TTCCGCATCG GCCCTGCTCC CGCCTTTGCT GGGGACACCA TCTCCCTGAC CATCACGAAG 
GGCAATGAGG AGGGCTACTT CGTCACACGC AGACTCAATG CCTACACTGG TGTGGTATCC 
CTGCAGCGGT CTGTTCTGGA GCCGCGGGAC TTTGCCCTAG ATGTGGAGAT GAAGCTTTGG 
CGCCAGGGCT CTGTCACTAC CTTCCTGGCC AAGATGTACA TCTTCTTCAC CACTTTTGCC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fbln2 ( NM_001081437.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074906.1 Mus musculus fibulin 2 (Fbln2), transcript variant 2, mRNA.

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CloneID	OMu02441
Clone ID Related Accession (Same CDS sequence)	XM_030255156.1 , NM_001081437.1
Accession Version	NM_001081437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Mus musculus(house mouse)
Product	fibulin-2 isoform b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC131764.3, AK168688.1 and BY690088.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform b, which lacks an internal segment and is shorter, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK168688.1, AK161522.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTCC AGGAATCTGC AGGAGTGTGG CTGGCCTTGG CTCTGGTCAC AGCTCTGACT 
CCGAGCCCAA GCATGGCCGT CCCGTGGCAG GATTGCACAG GTGCTGAGTG TCCCCTGCTG 
GAGAACTGCA TCGAGGAGGC GTTGGAGCCA GGAGCCTGCT GTGCTACCTG TGTGCAGCAG 
GGCTGTGCCT GCGAAGGCTA CCAGTACTAC GACTGCGTAC AAGGAGGGTT TGTGGATGGC 
CGTGTACCAG CCGGCCAGTC CTACTTTGTG GACTTTGGCA GCACCGAGTG CTCCTGCCCA 
CCCGGTGGTG GCAAGATCAG CTGCCAGTTC ATGCTGTGCC CTGAGCTACC ACCCAACTGC 
ATTGAAGCTG TAGTAGTAGC CGACAGCTGC CCGCAGTGTG GCCAGGTGGG CTGTGTCCAC 
AGTGGCCGTA AGTATGCTGC TGGCCATACC GTCCACCTCT CATCCTGCCG GGCTTGCCAC 
TGTCCTGACG CTGGTGGTGA GCTCATTTGC TACCAGCTTC CTGGTTGCCA TGGGAACTTC 
TCGGATGCTG AGGAGGGTGA CTCTGAGAGG CAGTATGAAG ACCCCTACAG CTATGACCAG 
GAGGTGGCAG AGGCAGAAGC TACTACGGCC ATTGTGAACG AGGTCCAGGC AGGTGCAGAG 
GGCCCCCCAG CTGCTCTGGG AGGAGGGAAT CTGCCACCGT CCTCCATCAG AGTAACACCC 
TGGCCAGTTG CCCTTCCCAG ACCCACAGCA GCTGCTGCCC TGGGTCCCCC AGCACCTGTG 
CAGGCCAAAG CCAGGAGAGT GACATTGGAC ACTGAAGAGG ATGAGGAAGA AGAAGAGGAG 
GAGACACTAG TCACTGAGCC ACCAACAGCA GGCAGTCCTG GAAGGCTGGA CAGCCTGCCC 
ACAAGATCCC CAGCCAGACC TGGTTTCCCT GTCCAGGAGA AGGAGGCTGA AGCTAAGGCA 
GGGCCTGAGG AAAACCTTAT TCCTGATGCC CAGGTCACTC CTCGAAGTGT CATGCAGGAG 
GGTGCTGCAC CTCTGCCAAG GTCAGGCCTG GCTGCTCTCA GTCCATCTCT GGCCACTGAC 
AGCTCTTCAG AGGACCCAGT AAAGCCCAGC GATCACCCCA CTCTATCTAC ACTGCCACCT 
GACCGAGCCC AGGTCTCCCC CTCCCCAGAG ACACCTGAAG AAATACCCCA GCATCCACAG 
TTGCTACCTC GATTCCGGGC AGAAGAAGAC ATAGACCCCA ACTCAGTACA TTCTGTCCCC 
AGAGGTGATC TTGATGGCTC CACCAAGGAC CTAATCGAGA CCTGTTGTGC AGCGGGCCAG 
CAGTGGGCCA TCGACAATGA TGAGTGCCAG GAGATCCCCG AGAATGGTGC CCAGAGTGAC 
ATCTGTAGGA TAGCCCAGAG GCAATGCTGC ATCTCCTACT TGAAAGAGAA GAGCTGTGTG 
GCTGGGGTCA TGGGAGCCAA GGAGGGTGAG ACCTGTGGAG CAGAGGACAA TGATACCTGC 
GGCGTCTCAC TCTACAAGCA ATGCTGTGAC TGCTGTGGCC TGGGACTCCG AGTGCGGGCT 
GAGGGCCAGT CCTGTGAGTC CAACCCCAAC CTGGGCTATC CCTGCAACCA TGTCATGCTC 
TCCTGCTGTG AAGGTGAAGA GCCCCTCATA GTGCCTGAGG TCCGCCGGCC ACCAGAGCCC 
GAGGCTGCTC CTCGCAGAGT TTCAGAGATG GAGATGGCAA GCCGGGAGGC CCTGTCACTG 
GGCACAGAGG CTGAACTGCC CAATAGCCTG CCGGGAGATG ACCAGGATGA GTGCCTGATG 
CTCCCTGGGG AGCTTTGCCA GCATCTTTGC ATCAACACCG TCGGCTCCTA CCGCTGTGCC 
TGCTTCCCTG GATTCGAGCT GCAGGGTGAT GGCCGTACCT GCCGCCCAGA TAGAGGTGCC 
CCACAGTTGG ACACTGCACG AGAGTCTGCA CCGAGGTCTG AGTCTGCCCA GGTATCTCCC 
AACACCATCC CACTGCCGGT GCCACAGCCC AACACCTGCA AAGACAATGG GCCCTGCCGT 
CAGGTGTGTC GTGTTGTTGG GGACACAGCT ATGTGCTCCT GCTTCCCTGG CTACGCCATC 
ATGGCAGATG GTGTGTCCTG TGAAGACATC AATGAGTGTG TGACAGACCT GCACACGTGC 
ACCCGGGCCG AGCACTGTGT GAACACGCCA GGTTCCTTCC AATGCTACAA GGCGCTCACC 
TGTGAGCCAG GCTATGTCCT CACAGATGGT GAATGCACAG ACGTGGACGA ATGTGTGACG 
GGCACACACA ACTGCCAGGC AGGCTTCTCA TGCCAGAACA CCAAGGGCTC CTTCTACTGC 
CAGGCCCGGC AACGCTGCAT GGATGGCTTC TTGCAGGACC CCGAGGGCAA CTGTGTGGAC 
ATCAACGAAT GCACATCGTT ACTGGAGCCT TGCCGCTCAG GGTTCAGCTG CATCAACACA 
GTGGGCTCCT ACACCTGTCA GAGGAACCCA CTGGTCTGCG GTCGCGGTTA CCATGCTAAC 
GAGGAGGGCT CTGAATGTGT GGATGTGAAT GAGTGTGAGA CAGGTGTGCA TCGCTGTGGC 
GAGGGCCAAC TGTGCTATAA CCTCCCTGGA TCCTACCGCT GTGACTGCAA GCCCGGCTTC 
CAGAGGGATG CATTCGGCAG GACTTGCATT GATGTGAACG AATGCTGGGT CTCGCCGGGC 
CGCCTGTGCC AGCACACATG TGAGAACACA CCGGGCTCCT ACCGCTGCTC CTGCGCTGCT 
GGCTTCCTTT TGGCCGCAGA TGGCAAACAT TGTGAAGATG TGAACGAGTG CGAGACTCGG 
CGCTGCAGCC AGGAATGTGC CAACATCTAT GGCTCCTATC AGTGCTACTG CCGTCAGGGC 
TACCAGCTGG CAGAGGATGG GCATACCTGC ACAGACATCG ATGAGTGTGC ACAGGGCGCG 
GGCATTCTCT GTACCTTCCG CTGTGTCAAC GTGCCTGGGA GCTACCAGTG TGCATGCCCA 
GAGCAAGGGT ATACAATGAT GGCCAACGGG AGGTCCTGCA AGGACCTGGA TGAGTGTGCA 
CTGGGCACCC ACAACTGCTC TGAGGCTGAG ACCTGCCACA ATATCCAGGG GAGTTTCCGC 
TGCCTGCGCT TTGATTGTCC ACCCAACTAT GTCCGTGTCT CAGAAACGAA GTGCGAGCGC 
ACCACATGCC AGGATATCAC GGAATGTCAA ACCTCACCAG CTCGCATCAC GCACTACCAG 
CTCAATTTCC AGACAGGCCT ACTGGTACCT GCACATATCT TCCGCATCGG CCCTGCTCCC 
GCCTTTGCTG GGGACACCAT CTCCCTGACC ATCACGAAGG GCAATGAGGA GGGCTACTTC 
GTCACACGCA GACTCAATGC CTACACTGGT GTGGTATCCC TGCAGCGGTC TGTTCTGGAG 
CCGCGGGACT TTGCCCTAGA TGTGGAGATG AAGCTTTGGC GCCAGGGCTC TGTCACTACC 
TTCCTGGCCA AGATGTACAT CTTCTTCACC ACTTTTGCCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074906.1
CDS	151..3675
Translation	MLLQESAGVWLALALVTALTPSPSMAVPWQDCTGAECPLLENCIEEALEPGACCATCVQQGCACEGYQYYDCVQGGFVDGRVPAGQSYFVDFGSTECSCPPGGGKISCQFMLCPELPPNCIEAVVVADSCPQCGQVGCVHSGRKYAAGHTVHLSSCRACHCPDAGGELICYQLPGCHGNFSDAEEGDSERQYEDPYSYDQEVAEAEATTAIVNEVQAGAEGPPAALGGGNLPPSSIRVTPWPVALPRPTAAAALGPPAPVQAKARRVTLDTEEDEEEEEEETLVTEPPTAGSPGRLDSLPTRSPARPGFPVQEKEAEAKAGPEENLIPDAQVTPRSVMQEGAAPLPRSGLAALSPSLATDSSSEDPVKPSDHPTLSTLPPDRAQVSPSPETPEEIPQHPQLLPRFRAEEDIDPNSVHSVPRGDLDGSTKDLIETCCAAGQQWAIDNDECQEIPENGAQSDICRIAQRQCCISYLKEKSCVAGVMGAKEGETCGAEDNDTCGVSLYKQCCDCCGLGLRVRAEGQSCESNPNLGYPCNHVMLSCCEGEEPLIVPEVRRPPEPEAAPRRVSEMEMASREALSLGTEAELPNSLPGDDQDECLMLPGELCQHLCINTVGSYRCACFPGFELQGDGRTCRPDRGAPQLDTARESAPRSESAQVSPNTIPLPVPQPNTCKDNGPCRQVCRVVGDTAMCSCFPGYAIMADGVSCEDINECVTDLHTCTRAEHCVNTPGSFQCYKALTCEPGYVLTDGECTDVDECVTGTHNCQAGFSCQNTKGSFYCQARQRCMDGFLQDPEGNCVDINECTSLLEPCRSGFSCINTVGSYTCQRNPLVCGRGYHANEEGSECVDVNECETGVHRCGEGQLCYNLPGSYRCDCKPGFQRDAFGRTCIDVNECWVSPGRLCQHTCENTPGSYRCSCAAGFLLAADGKHCEDVNECETRRCSQECANIYGSYQCYCRQGYQLAEDGHTCTDIDECAQGAGILCTFRCVNVPGSYQCACPEQGYTMMANGRSCKDLDECALGTHNCSEAETCHNIQGSFRCLRFDCPPNYVRVSETKCERTTCQDITECQTSPARITHYQLNFQTGLLVPAHIFRIGPAPAFAGDTISLTITKGNEEGYFVTRRLNAYTGVVSLQRSVLEPRDFALDVEMKLWRQGSVTTFLAKMYIFFTTFAP

Target ORF information:

RefSeq Version	NM_001081437.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus fibulin 2 (Fbln2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081437.1

ORF Insert Sequence:

ATGCTGCTCC AGGAATCTGC AGGAGTGTGG CTGGCCTTGG CTCTGGTCAC AGCTCTGACT 
CCGAGCCCAA GCATGGCCGT CCCGTGGCAG GATTGCACAG GTGCTGAGTG TCCCCTGCTG 
GAGAACTGCA TCGAGGAGGC GTTGGAGCCA GGAGCCTGCT GTGCTACCTG TGTGCAGCAG 
GGCTGTGCCT GCGAAGGCTA CCAGTACTAC GACTGCGTAC AAGGAGGGTT TGTGGATGGC 
CGTGTACCAG CCGGCCAGTC CTACTTTGTG GACTTTGGCA GCACCGAGTG CTCCTGCCCA 
CCCGGTGGTG GCAAGATCAG CTGCCAGTTC ATGCTGTGCC CTGAGCTACC ACCCAACTGC 
ATTGAAGCTG TAGTAGTAGC CGACAGCTGC CCGCAGTGTG GCCAGGTGGG CTGTGTCCAC 
AGTGGCCGTA AGTATGCTGC TGGCCATACC GTCCACCTCT CATCCTGCCG GGCTTGCCAC 
TGTCCTGACG CTGGTGGTGA GCTCATTTGC TACCAGCTTC CTGGTTGCCA TGGGAACTTC 
TCGGATGCTG AGGAGGGTGA CTCTGAGAGG CAGTATGAAG ACCCCTACAG CTATGACCAG 
GAGGTGGCAG AGGCAGAAGC TACTACGGCC ATTGTGAACG AGGTCCAGGC AGGTGCAGAG 
GGCCCCCCAG CTGCTCTGGG AGGAGGGAAT CTGCCACCGT CCTCCATCAG AGTAACACCC 
TGGCCAGTTG CCCTTCCCAG ACCCACAGCA GCTGCTGCCC TGGGTCCCCC AGCACCTGTG 
CAGGCCAAAG CCAGGAGAGT GACATTGGAC ACTGAAGAGG ATGAGGAAGA AGAAGAGGAG 
GAGACACTAG TCACTGAGCC ACCAACAGCA GGCAGTCCTG GAAGGCTGGA CAGCCTGCCC 
ACAAGATCCC CAGCCAGACC TGGTTTCCCT GTCCAGGAGA AGGAGGCTGA AGCTAAGGCA 
GGGCCTGAGG AAAACCTTAT TCCTGATGCC CAGGTCACTC CTCGAAGTGT CATGCAGGAG 
GGTGCTGCAC CTCTGCCAAG GTCAGGCCTG GCTGCTCTCA GTCCATCTCT GGCCACTGAC 
AGCTCTTCAG AGGACCCAGT AAAGCCCAGC GATCACCCCA CTCTATCTAC ACTGCCACCT 
GACCGAGCCC AGGTCTCCCC CTCCCCAGAG ACACCTGAAG AAATACCCCA GCATCCACAG 
TTGCTACCTC GATTCCGGGC AGAAGAAGAC ATAGACCCCA ACTCAGTACA TTCTGTCCCC 
AGAGGTGATC TTGATGGCTC CACCAAGGAC CTAATCGAGA CCTGTTGTGC AGCGGGCCAG 
CAGTGGGCCA TCGACAATGA TGAGTGCCAG GAGATCCCCG AGAATGGTGC CCAGAGTGAC 
ATCTGTAGGA TAGCCCAGAG GCAATGCTGC ATCTCCTACT TGAAAGAGAA GAGCTGTGTG 
GCTGGGGTCA TGGGAGCCAA GGAGGGTGAG ACCTGTGGAG CAGAGGACAA TGATACCTGC 
GGCGTCTCAC TCTACAAGCA ATGCTGTGAC TGCTGTGGCC TGGGACTCCG AGTGCGGGCT 
GAGGGCCAGT CCTGTGAGTC CAACCCCAAC CTGGGCTATC CCTGCAACCA TGTCATGCTC 
TCCTGCTGTG AAGGTGAAGA GCCCCTCATA GTGCCTGAGG TCCGCCGGCC ACCAGAGCCC 
GAGGCTGCTC CTCGCAGAGT TTCAGAGATG GAGATGGCAA GCCGGGAGGC CCTGTCACTG 
GGCACAGAGG CTGAACTGCC CAATAGCCTG CCGGGAGATG ACCAGGATGA GTGCCTGATG 
CTCCCTGGGG AGCTTTGCCA GCATCTTTGC ATCAACACCG TCGGCTCCTA CCGCTGTGCC 
TGCTTCCCTG GATTCGAGCT GCAGGGTGAT GGCCGTACCT GCCGCCCAGA TAGAGGTGCC 
CCACAGTTGG ACACTGCACG AGAGTCTGCA CCGAGGTCTG AGTCTGCCCA GGTATCTCCC 
AACACCATCC CACTGCCGGT GCCACAGCCC AACACCTGCA AAGACAATGG GCCCTGCCGT 
CAGGTGTGTC GTGTTGTTGG GGACACAGCT ATGTGCTCCT GCTTCCCTGG CTACGCCATC 
ATGGCAGATG GTGTGTCCTG TGAAGACATC AATGAGTGTG TGACAGACCT GCACACGTGC 
ACCCGGGCCG AGCACTGTGT GAACACGCCA GGTTCCTTCC AATGCTACAA GGCGCTCACC 
TGTGAGCCAG GCTATGTCCT CACAGATGGT GAATGCACAG ACGTGGACGA ATGTGTGACG 
GGCACACACA ACTGCCAGGC AGGCTTCTCA TGCCAGAACA CCAAGGGCTC CTTCTACTGC 
CAGGCCCGGC AACGCTGCAT GGATGGCTTC TTGCAGGACC CCGAGGGCAA CTGTGTGGAC 
ATCAACGAAT GCACATCGTT ACTGGAGCCT TGCCGCTCAG GGTTCAGCTG CATCAACACA 
GTGGGCTCCT ACACCTGTCA GAGGAACCCA CTGGTCTGCG GTCGCGGTTA CCATGCTAAC 
GAGGAGGGCT CTGAATGTGT GGATGTGAAT GAGTGTGAGA CAGGTGTGCA TCGCTGTGGC 
GAGGGCCAAC TGTGCTATAA CCTCCCTGGA TCCTACCGCT GTGACTGCAA GCCCGGCTTC 
CAGAGGGATG CATTCGGCAG GACTTGCATT GATGTGAACG AATGCTGGGT CTCGCCGGGC 
CGCCTGTGCC AGCACACATG TGAGAACACA CCGGGCTCCT ACCGCTGCTC CTGCGCTGCT 
GGCTTCCTTT TGGCCGCAGA TGGCAAACAT TGTGAAGATG TGAACGAGTG CGAGACTCGG 
CGCTGCAGCC AGGAATGTGC CAACATCTAT GGCTCCTATC AGTGCTACTG CCGTCAGGGC 
TACCAGCTGG CAGAGGATGG GCATACCTGC ACAGACATCG ATGAGTGTGC ACAGGGCGCG 
GGCATTCTCT GTACCTTCCG CTGTGTCAAC GTGCCTGGGA GCTACCAGTG TGCATGCCCA 
GAGCAAGGGT ATACAATGAT GGCCAACGGG AGGTCCTGCA AGGACCTGGA TGAGTGTGCA 
CTGGGCACCC ACAACTGCTC TGAGGCTGAG ACCTGCCACA ATATCCAGGG GAGTTTCCGC 
TGCCTGCGCT TTGATTGTCC ACCCAACTAT GTCCGTGTCT CAGAAACGAA GTGCGAGCGC 
ACCACATGCC AGGATATCAC GGAATGTCAA ACCTCACCAG CTCGCATCAC GCACTACCAG 
CTCAATTTCC AGACAGGCCT ACTGGTACCT GCACATATCT TCCGCATCGG CCCTGCTCCC 
GCCTTTGCTG GGGACACCAT CTCCCTGACC ATCACGAAGG GCAATGAGGA GGGCTACTTC 
GTCACACGCA GACTCAATGC CTACACTGGT GTGGTATCCC TGCAGCGGTC TGTTCTGGAG 
CCGCGGGACT TTGCCCTAGA TGTGGAGATG AAGCTTTGGC GCCAGGGCTC TGTCACTACC 
TTCCTGGCCA AGATGTACAT CTTCTTCACC ACTTTTGCCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fbln2 ( XM_006505530.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505593.1 Mus musculus fibulin 2 (Fbln2), transcript variant X1, mRNA.

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CloneID	OMu01192
Clone ID Related Accession (Same CDS sequence)	XM_006505530.4 , NM_007992.2 , XM_006505530.3
Accession Version	XM_006505530.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	fibulin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006505530.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCC AGGAATCTGC AGGAGTGTGG CTGGCCTTGG CTCTGGTCAC AGCTCTGACT 
CCGAGCCCAA GCATGGCCGT CCCGTGGCAG GATTGCACAG GTGCTGAGTG TCCCCTGCTG 
GAGAACTGCA TCGAGGAGGC GTTGGAGCCA GGAGCCTGCT GTGCTACCTG TGTGCAGCAG 
GGCTGTGCCT GCGAAGGCTA CCAGTACTAC GACTGCGTAC AAGGAGGGTT TGTGGATGGC 
CGTGTACCAG CCGGCCAGTC CTACTTTGTG GACTTTGGCA GCACCGAGTG CTCCTGCCCA 
CCCGGTGGTG GCAAGATCAG CTGCCAGTTC ATGCTGTGCC CTGAGCTACC ACCCAACTGC 
ATTGAAGCTG TAGTAGTAGC CGACAGCTGC CCGCAGTGTG GCCAGGTGGG CTGTGTCCAC 
AGTGGCCGTA AGTATGCTGC TGGCCATACC GTCCACCTCT CATCCTGCCG GGCTTGCCAC 
TGTCCTGACG CTGGTGGTGA GCTCATTTGC TACCAGCTTC CTGGTTGCCA TGGGAACTTC 
TCGGATGCTG AGGAGGGTGA CTCTGAGAGG CAGTATGAAG ACCCCTACAG CTATGACCAG 
GAGGTGGCAG AGGCAGAAGC TACTACGGCC ATTGTGAACG AGGTCCAGGC AGGTGCAGAG 
GGCCCCCCAG CTGCTCTGGG AGGAGGGAAT CTGCCACCGT CCTCCATCAG AGTAACACCC 
TGGCCAGTTG CCCTTCCCAG ACCCACAGCA GCTGCTGCCC TGGGTCCCCC AGCACCTGTG 
CAGGCCAAAG CCAGGAGAGT GACATTGGAC ACTGAAGAGG ATGAGGAAGA AGAAGAGGAG 
GAGACACTAG TCACTGAGCC ACCAACAGCA GGCAGTCCTG GAAGGCTGGA CAGCCTGCCC 
ACAAGATCCC CAGCCAGACC TGGTTTCCCT GTCCAGGAGA AGGAGGCTGA AGCTAAGGCA 
GGGCCTGAGG AAAACCTTAT TCCTGATGCC CAGGTCACTC CTCGAAGTGT CATGCAGGAG 
GGTGCTGCAC CTCTGCCAAG GTCAGGCCTG GCTGCTCTCA GTCCATCTCT GGCCACTGAC 
AGCTCTTCAG AGGACCCAGT AAAGCCCAGC GATCACCCCA CTCTATCTAC ACTGCCACCT 
GACCGAGCCC AGGTCTCCCC CTCCCCAGAG ACACCTGAAG AAATACCCCA GCATCCACAG 
TTGCTACCTC GATTCCGGGC AGAAGAAGAC ATAGACCCCA ACTCAGTACA TTCTGTCCCC 
AGAGGTGATC TTGATGGCTC CACCAAGGAC CTAATCGAGA CCTGTTGTGC AGCGGGCCAG 
CAGTGGGCCA TCGACAATGA TGAGTGCCAG GAGATCCCCG AGAATGGTGC CCAGAGTGAC 
ATCTGTAGGA TAGCCCAGAG GCAATGCTGC ATCTCCTACT TGAAAGAGAA GAGCTGTGTG 
GCTGGGGTCA TGGGAGCCAA GGAGGGTGAG ACCTGTGGAG CAGAGGACAA TGATACCTGC 
GGCGTCTCAC TCTACAAGCA ATGCTGTGAC TGCTGTGGCC TGGGACTCCG AGTGCGGGCT 
GAGGGCCAGT CCTGTGAGTC CAACCCCAAC CTGGGCTATC CCTGCAACCA TGTCATGCTC 
TCCTGCTGTG AAGGTGAAGA GCCCCTCATA GTGCCTGAGG TCCGCCGGCC ACCAGAGCCC 
GAGGCTGCTC CTCGCAGAGT TTCAGAGATG GAGATGGCAA GCCGGGAGGC CCTGTCACTG 
GGCACAGAGG CTGAACTGCC CAATAGCCTG CCGGGAGATG ACCAGGATGA GTGCCTGATG 
CTCCCTGGGG AGCTTTGCCA GCATCTTTGC ATCAACACCG TCGGCTCCTA CCGCTGTGCC 
TGCTTCCCTG GATTCGAGCT GCAGGGTGAT GGCCGTACCT GCCGCCCAGA TAGAGGTGCC 
CCACAGTTGG ACACTGCACG AGAGTCTGCA CCGAGGTCTG AGTCTGCCCA GGTATCTCCC 
AACACCATCC CACTGCCGGT GCCACAGCCC AACACCTGCA AAGACAATGG GCCCTGCCGT 
CAGGTGTGTC GTGTTGTTGG GGACACAGCT ATGTGCTCCT GCTTCCCTGG CTACGCCATC 
ATGGCAGATG GTGTGTCCTG TGAAGACCAA GACGAGTGTT TGATGGGCAC TCACGATTGT 
AGCTGGAAAC AGTTCTGTGT GAACACCCTG GGATCCTTCT ACTGTGTCAA CCACACAGTG 
CTGTGTGCAG AGGGCTATAT CCTCAACGCA CATAGGAAGT GTGTGGACAT CAATGAGTGT 
GTGACAGACC TGCACACGTG CACCCGGGCC GAGCACTGTG TGAACACGCC AGGTTCCTTC 
CAATGCTACA AGGCGCTCAC CTGTGAGCCA GGCTATGTCC TCACAGATGG TGAATGCACA 
GACGTGGACG AATGTGTGAC GGGCACACAC AACTGCCAGG CAGGCTTCTC ATGCCAGAAC 
ACCAAGGGCT CCTTCTACTG CCAGGCCCGG CAACGCTGCA TGGATGGCTT CTTGCAGGAC 
CCCGAGGGCA ACTGTGTGGA CATCAACGAA TGCACATCGT TACTGGAGCC TTGCCGCTCA 
GGGTTCAGCT GCATCAACAC AGTGGGCTCC TACACCTGTC AGAGGAACCC ACTGGTCTGC 
GGTCGCGGTT ACCATGCTAA CGAGGAGGGC TCTGAATGTG TGGATGTGAA TGAGTGTGAG 
ACAGGTGTGC ATCGCTGTGG CGAGGGCCAA CTGTGCTATA ACCTCCCTGG ATCCTACCGC 
TGTGACTGCA AGCCCGGCTT CCAGAGGGAT GCATTCGGCA GGACTTGCAT TGATGTGAAC 
GAATGCTGGG TCTCGCCGGG CCGCCTGTGC CAGCACACAT GTGAGAACAC ACCGGGCTCC 
TACCGCTGCT CCTGCGCTGC TGGCTTCCTT TTGGCCGCAG ATGGCAAACA TTGTGAAGAT 
GTGAACGAGT GCGAGACTCG GCGCTGCAGC CAGGAATGTG CCAACATCTA TGGCTCCTAT 
CAGTGCTACT GCCGTCAGGG CTACCAGCTG GCAGAGGATG GGCATACCTG CACAGACATC 
GATGAGTGTG CACAGGGCGC GGGCATTCTC TGTACCTTCC GCTGTGTCAA CGTGCCTGGG 
AGCTACCAGT GTGCATGCCC AGAGCAAGGG TATACAATGA TGGCCAACGG GAGGTCCTGC 
AAGGACCTGG ATGAGTGTGC ACTGGGCACC CACAACTGCT CTGAGGCTGA GACCTGCCAC 
AATATCCAGG GGAGTTTCCG CTGCCTGCGC TTTGATTGTC CACCCAACTA TGTCCGTGTC 
TCAGAAACGA AGTGCGAGCG CACCACATGC CAGGATATCA CGGAATGTCA AACCTCACCA 
GCTCGCATCA CGCACTACCA GCTCAATTTC CAGACAGGCC TACTGGTACC TGCACATATC 
TTCCGCATCG GCCCTGCTCC CGCCTTTGCT GGGGACACCA TCTCCCTGAC CATCACGAAG 
GGCAATGAGG AGGGCTACTT CGTCACACGC AGACTCAATG CCTACACTGG TGTGGTATCC 
CTGCAGCGGT CTGTTCTGGA GCCGCGGGAC TTTGCCCTAG ATGTGGAGAT GAAGCTTTGG 
CGCCAGGGCT CTGTCACTAC CTTCCTGGCC AAGATGTACA TCTTCTTCAC CACTTTTGCC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505593.1
CDS	305..3970
Translation	MLLQESAGVWLALALVTALTPSPSMAVPWQDCTGAECPLLENCIEEALEPGACCATCVQQGCACEGYQYYDCVQGGFVDGRVPAGQSYFVDFGSTECSCPPGGGKISCQFMLCPELPPNCIEAVVVADSCPQCGQVGCVHSGRKYAAGHTVHLSSCRACHCPDAGGELICYQLPGCHGNFSDAEEGDSERQYEDPYSYDQEVAEAEATTAIVNEVQAGAEGPPAALGGGNLPPSSIRVTPWPVALPRPTAAAALGPPAPVQAKARRVTLDTEEDEEEEEEETLVTEPPTAGSPGRLDSLPTRSPARPGFPVQEKEAEAKAGPEENLIPDAQVTPRSVMQEGAAPLPRSGLAALSPSLATDSSSEDPVKPSDHPTLSTLPPDRAQVSPSPETPEEIPQHPQLLPRFRAEEDIDPNSVHSVPRGDLDGSTKDLIETCCAAGQQWAIDNDECQEIPENGAQSDICRIAQRQCCISYLKEKSCVAGVMGAKEGETCGAEDNDTCGVSLYKQCCDCCGLGLRVRAEGQSCESNPNLGYPCNHVMLSCCEGEEPLIVPEVRRPPEPEAAPRRVSEMEMASREALSLGTEAELPNSLPGDDQDECLMLPGELCQHLCINTVGSYRCACFPGFELQGDGRTCRPDRGAPQLDTARESAPRSESAQVSPNTIPLPVPQPNTCKDNGPCRQVCRVVGDTAMCSCFPGYAIMADGVSCEDQDECLMGTHDCSWKQFCVNTLGSFYCVNHTVLCAEGYILNAHRKCVDINECVTDLHTCTRAEHCVNTPGSFQCYKALTCEPGYVLTDGECTDVDECVTGTHNCQAGFSCQNTKGSFYCQARQRCMDGFLQDPEGNCVDINECTSLLEPCRSGFSCINTVGSYTCQRNPLVCGRGYHANEEGSECVDVNECETGVHRCGEGQLCYNLPGSYRCDCKPGFQRDAFGRTCIDVNECWVSPGRLCQHTCENTPGSYRCSCAAGFLLAADGKHCEDVNECETRRCSQECANIYGSYQCYCRQGYQLAEDGHTCTDIDECAQGAGILCTFRCVNVPGSYQCACPEQGYTMMANGRSCKDLDECALGTHNCSEAETCHNIQGSFRCLRFDCPPNYVRVSETKCERTTCQDITECQTSPARITHYQLNFQTGLLVPAHIFRIGPAPAFAGDTISLTITKGNEEGYFVTRRLNAYTGVVSLQRSVLEPRDFALDVEMKLWRQGSVTTFLAKMYIFFTTFAP

Target ORF information:

RefSeq Version	XM_006505530.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus fibulin 2 (Fbln2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505530.3

ORF Insert Sequence:

ATGCTGCTCC AGGAATCTGC AGGAGTGTGG CTGGCCTTGG CTCTGGTCAC AGCTCTGACT 
CCGAGCCCAA GCATGGCCGT CCCGTGGCAG GATTGCACAG GTGCTGAGTG TCCCCTGCTG 
GAGAACTGCA TCGAGGAGGC GTTGGAGCCA GGAGCCTGCT GTGCTACCTG TGTGCAGCAG 
GGCTGTGCCT GCGAAGGCTA CCAGTACTAC GACTGCGTAC AAGGAGGGTT TGTGGATGGC 
CGTGTACCAG CCGGCCAGTC CTACTTTGTG GACTTTGGCA GCACCGAGTG CTCCTGCCCA 
CCCGGTGGTG GCAAGATCAG CTGCCAGTTC ATGCTGTGCC CTGAGCTACC ACCCAACTGC 
ATTGAAGCTG TAGTAGTAGC CGACAGCTGC CCGCAGTGTG GCCAGGTGGG CTGTGTCCAC 
AGTGGCCGTA AGTATGCTGC TGGCCATACC GTCCACCTCT CATCCTGCCG GGCTTGCCAC 
TGTCCTGACG CTGGTGGTGA GCTCATTTGC TACCAGCTTC CTGGTTGCCA TGGGAACTTC 
TCGGATGCTG AGGAGGGTGA CTCTGAGAGG CAGTATGAAG ACCCCTACAG CTATGACCAG 
GAGGTGGCAG AGGCAGAAGC TACTACGGCC ATTGTGAACG AGGTCCAGGC AGGTGCAGAG 
GGCCCCCCAG CTGCTCTGGG AGGAGGGAAT CTGCCACCGT CCTCCATCAG AGTAACACCC 
TGGCCAGTTG CCCTTCCCAG ACCCACAGCA GCTGCTGCCC TGGGTCCCCC AGCACCTGTG 
CAGGCCAAAG CCAGGAGAGT GACATTGGAC ACTGAAGAGG ATGAGGAAGA AGAAGAGGAG 
GAGACACTAG TCACTGAGCC ACCAACAGCA GGCAGTCCTG GAAGGCTGGA CAGCCTGCCC 
ACAAGATCCC CAGCCAGACC TGGTTTCCCT GTCCAGGAGA AGGAGGCTGA AGCTAAGGCA 
GGGCCTGAGG AAAACCTTAT TCCTGATGCC CAGGTCACTC CTCGAAGTGT CATGCAGGAG 
GGTGCTGCAC CTCTGCCAAG GTCAGGCCTG GCTGCTCTCA GTCCATCTCT GGCCACTGAC 
AGCTCTTCAG AGGACCCAGT AAAGCCCAGC GATCACCCCA CTCTATCTAC ACTGCCACCT 
GACCGAGCCC AGGTCTCCCC CTCCCCAGAG ACACCTGAAG AAATACCCCA GCATCCACAG 
TTGCTACCTC GATTCCGGGC AGAAGAAGAC ATAGACCCCA ACTCAGTACA TTCTGTCCCC 
AGAGGTGATC TTGATGGCTC CACCAAGGAC CTAATCGAGA CCTGTTGTGC AGCGGGCCAG 
CAGTGGGCCA TCGACAATGA TGAGTGCCAG GAGATCCCCG AGAATGGTGC CCAGAGTGAC 
ATCTGTAGGA TAGCCCAGAG GCAATGCTGC ATCTCCTACT TGAAAGAGAA GAGCTGTGTG 
GCTGGGGTCA TGGGAGCCAA GGAGGGTGAG ACCTGTGGAG CAGAGGACAA TGATACCTGC 
GGCGTCTCAC TCTACAAGCA ATGCTGTGAC TGCTGTGGCC TGGGACTCCG AGTGCGGGCT 
GAGGGCCAGT CCTGTGAGTC CAACCCCAAC CTGGGCTATC CCTGCAACCA TGTCATGCTC 
TCCTGCTGTG AAGGTGAAGA GCCCCTCATA GTGCCTGAGG TCCGCCGGCC ACCAGAGCCC 
GAGGCTGCTC CTCGCAGAGT TTCAGAGATG GAGATGGCAA GCCGGGAGGC CCTGTCACTG 
GGCACAGAGG CTGAACTGCC CAATAGCCTG CCGGGAGATG ACCAGGATGA GTGCCTGATG 
CTCCCTGGGG AGCTTTGCCA GCATCTTTGC ATCAACACCG TCGGCTCCTA CCGCTGTGCC 
TGCTTCCCTG GATTCGAGCT GCAGGGTGAT GGCCGTACCT GCCGCCCAGA TAGAGGTGCC 
CCACAGTTGG ACACTGCACG AGAGTCTGCA CCGAGGTCTG AGTCTGCCCA GGTATCTCCC 
AACACCATCC CACTGCCGGT GCCACAGCCC AACACCTGCA AAGACAATGG GCCCTGCCGT 
CAGGTGTGTC GTGTTGTTGG GGACACAGCT ATGTGCTCCT GCTTCCCTGG CTACGCCATC 
ATGGCAGATG GTGTGTCCTG TGAAGACCAA GACGAGTGTT TGATGGGCAC TCACGATTGT 
AGCTGGAAAC AGTTCTGTGT GAACACCCTG GGATCCTTCT ACTGTGTCAA CCACACAGTG 
CTGTGTGCAG AGGGCTATAT CCTCAACGCA CATAGGAAGT GTGTGGACAT CAATGAGTGT 
GTGACAGACC TGCACACGTG CACCCGGGCC GAGCACTGTG TGAACACGCC AGGTTCCTTC 
CAATGCTACA AGGCGCTCAC CTGTGAGCCA GGCTATGTCC TCACAGATGG TGAATGCACA 
GACGTGGACG AATGTGTGAC GGGCACACAC AACTGCCAGG CAGGCTTCTC ATGCCAGAAC 
ACCAAGGGCT CCTTCTACTG CCAGGCCCGG CAACGCTGCA TGGATGGCTT CTTGCAGGAC 
CCCGAGGGCA ACTGTGTGGA CATCAACGAA TGCACATCGT TACTGGAGCC TTGCCGCTCA 
GGGTTCAGCT GCATCAACAC AGTGGGCTCC TACACCTGTC AGAGGAACCC ACTGGTCTGC 
GGTCGCGGTT ACCATGCTAA CGAGGAGGGC TCTGAATGTG TGGATGTGAA TGAGTGTGAG 
ACAGGTGTGC ATCGCTGTGG CGAGGGCCAA CTGTGCTATA ACCTCCCTGG ATCCTACCGC 
TGTGACTGCA AGCCCGGCTT CCAGAGGGAT GCATTCGGCA GGACTTGCAT TGATGTGAAC 
GAATGCTGGG TCTCGCCGGG CCGCCTGTGC CAGCACACAT GTGAGAACAC ACCGGGCTCC 
TACCGCTGCT CCTGCGCTGC TGGCTTCCTT TTGGCCGCAG ATGGCAAACA TTGTGAAGAT 
GTGAACGAGT GCGAGACTCG GCGCTGCAGC CAGGAATGTG CCAACATCTA TGGCTCCTAT 
CAGTGCTACT GCCGTCAGGG CTACCAGCTG GCAGAGGATG GGCATACCTG CACAGACATC 
GATGAGTGTG CACAGGGCGC GGGCATTCTC TGTACCTTCC GCTGTGTCAA CGTGCCTGGG 
AGCTACCAGT GTGCATGCCC AGAGCAAGGG TATACAATGA TGGCCAACGG GAGGTCCTGC 
AAGGACCTGG ATGAGTGTGC ACTGGGCACC CACAACTGCT CTGAGGCTGA GACCTGCCAC 
AATATCCAGG GGAGTTTCCG CTGCCTGCGC TTTGATTGTC CACCCAACTA TGTCCGTGTC 
TCAGAAACGA AGTGCGAGCG CACCACATGC CAGGATATCA CGGAATGTCA AACCTCACCA 
GCTCGCATCA CGCACTACCA GCTCAATTTC CAGACAGGCC TACTGGTACC TGCACATATC 
TTCCGCATCG GCCCTGCTCC CGCCTTTGCT GGGGACACCA TCTCCCTGAC CATCACGAAG 
GGCAATGAGG AGGGCTACTT CGTCACACGC AGACTCAATG CCTACACTGG TGTGGTATCC 
CTGCAGCGGT CTGTTCTGGA GCCGCGGGAC TTTGCCCTAG ATGTGGAGAT GAAGCTTTGG 
CGCCAGGGCT CTGTCACTAC CTTCCTGGCC AAGATGTACA TCTTCTTCAC CACTTTTGCC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Development (Cambridge, England)144(17)3177-3188(2017 09)
Rutledge EA,Benazet JD,McMahon AP

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Fbln2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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