DACT2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DACT2
Entrez Gene ID	168002
Full Name	dishevelled binding antagonist of beta catenin 2
Synonyms	C6orf116,DAPPER2,DPR2,bA503C24.7
General protein information	Preferred Names dishevelled binding antagonist of beta catenin 2 Names dapper homolog 2 dapper antagonist of catenin 2 dapper homolog 2, antagonist of beta-catenin dapper, antagonist of beta-catenin, homolog 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q27
Disorder MIM:	608966

mRNA and Protein(s)

mRNA	Protein	Name
NM_001286350.1	NP_001273279.1	dapper homolog 2 isoform b
NM_214462.5	NP_999627.2	dapper homolog 2 isoform a
XM_011535507.2	XP_011533809.1	dapper homolog 2 isoform X1
NM_214462.4	NP_999627.2	dapper homolog 2 isoform a
NM_001286351.2	NP_001273280.1	dapper homolog 2 isoform c
NM_001286351.1	NP_001273280.1	dapper homolog 2 isoform c
NM_001286350.2	NP_001273279.1	dapper homolog 2 isoform b

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DACT2	XP_002690443.2
Pan troglodytes (chimpanzee)	DACT2	XP_001145122.2
Homo sapiens (human)	DACT2	NP_999627.2
	DACT2	XP_001083736.1
Mus musculus (house mouse)	Dact2	NP_766414.3
Rattus norvegicus (Norway rat)	Dact2	NP_001100934.2
Canis lupus familiaris (dog)	DACT2	XP_855310.3
Gallus gallus (chicken)	DACT2	XP_419602.4

Gene Ontology
Bibliography

Related articles in PubMed

DACT2 Epigenetic Stimulator Exerts Dual Efficacy for Colorectal Cancer Prevention and Treatment.
Lu L, Wang Y, Ou R, Feng Q, Ji L, Zheng H, Guo Y, Qi X, Kong AN, Liu Z
Pharmacological research(2017 12)

Methylation of DACT2 promotes breast cancer development by activating Wnt signaling.
Li J, Zhang M, He T, Li H, Cao T, Zheng L, Guo M
Scientific reports7(1)3325(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The simultaneous methylation of DACT1 and DACT2 may play important roles in progression of ESCC and may serve as prognostic methylation biomarkers for ESCC patients.
Title: Aberrant methylation of DACT1 and DACT2 are associated with tumor progression and poor prognosis in esophageal squamous cell carcinoma.

DACT2 is frequently methylated in human esophageal cancer and its expression is regulated by promoter region methylation. DACT2 suppresses esophageal cancer growth by inhibiting Wnt signaling.
Title: Methylation of DACT2 accelerates esophageal cancer development by activating Wnt signaling.

This study demonstrated that Genes overexpressed in Pilomyxoid Astrocytoma vs. Pilocytic Astrocytoma, ranked according to fold-change, included developmental genes H19, DACT2,COL2A1; COL1A1 and IMP3.
Title: Pilomyxoid Astrocytoma (PMA) Shows Significant Differences in Gene Expression vs. Pilocytic Astrocytoma (PA) and Variable Tendency Toward Maturation to PA.

There was no statistical difference between groups concerning DACT1 and DACT2 either in promoter hypermethylation or transcript levels. Age was associated with DACT2 promoter hypermethylation, especially over 56 years old.
Title: Expression and epigenetic regulation of DACT1 and DACT2 in oral squamous cell carcinoma.

DACT2 suppresses papillary thyroid cancer proliferation and metastasis by inhibiting Wnt signaling.
Title: Methylation of DACT2 promotes papillary thyroid cancer metastasis by activating Wnt signaling.

The following DACT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DACT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09961	NM_214462.5 Latest version!	Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu14407	NM_001286350.2 Latest version!	Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu77362	XM_011535507.2 Latest version!	Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu12028	NM_001286351.2 Latest version!	Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
View Old Accession Versions >>
OHu09961	NM_214462.4	Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu12028	NM_001286351.1	Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu14407	NM_001286350.1	Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DACT2 ( NM_214462.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_999627.2 Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09961
Clone ID Related Accession (Same CDS sequence)	NM_214462.5 , NM_214462.4
Accession Version	NM_214462.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Homo sapiens(human)
Product	dapper homolog 2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL606970.12, BC092498.1, BC111790.1, AF318336.1 and BX377941.2. On Nov 23, 2018 this sequence version replaced NM_214462.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF318336.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000366795.4/ ENSP00000355760.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGGACGC CGGGCGGACC CCCGGGGTCC GCGGGCTGGG ACCGCCGTAG GTTGGGCGCG 
AGGTTGCGCG CGGCGTTCGC GGGGCTGCAG GAGCTGCAGG GGCTGCGAGC CACGCAGCAG 
GAGCGGGTAC GGGGCGCCCT GGCCCTGCAG CCCCCGCCCG CGCCCGCCGC GCCCTGCGGC 
CCCCACGGCC TCCACGGCCC CGAGCAGCAG CTGGAGGCGG CGCTGGCCGC GCTGCAGGAG 
CAGCTGTCCC GGCTGAGACA ACAGGACATC GGCCTGAAGA CCCACCTGGA CCAGCTGGAC 
CTGCAGATTA GCAAGCTGCA GCTGGATGTG GGCACAGCCT CAGGGGAGGC CCTGGACAGC 
GACAGCAGGC CCAGCTCAGG CTTTTACGAG ATGAGCGACG GTGGATCCTG CTCCCTGTCC 
ACGTCCTGTG CCTCCGTCTG CAGTGACCAC ATCTCTCCCT CGCTGGGCAG TTTGTTGCCT 
GTGGCCCAGG CCCACAAGGC CAGGCCCAGC ATGGGGGACT GGAGGCCCCG GTCGGTTGAT 
GAGACTACTG TGCCAGCGTG GAGACCCCAG GCTACCGAGG AGGGCGCCAG GCCCCCAGGG 
AGCGTGGAGG ATGCAGGCCA GCCGTGGGGC ACATTCTGGC CCAGGCCTGT GTCTACAGGT 
GATCTTGACA GAGCCCTGCC GGCGGACACG GGGCTCCAGA AAGCCAGCGC GGACGCCGAG 
CTCCTCGGGC TCCTCTGCCA GGGGGTGGAT ATCCCGCTGC ACGTGCCGGA CCCCAAGTAT 
CGGCAGGACC TGGTGTCCCA GGGCGGCAGG GAGGTGTACC CGTACCCCAG CCCCCTGCAC 
GCCGTGGCTC TACAGAGCCC CCTGTTTGTC CTGACTAAGG AAACCCCACA GAGAGGTGGC 
CCCTCGTTCC CTAGGGAGAG CCCCAGGGGC CCCGCAGGTC TGAACACCAT CCAGACTGGG 
CCGGTCCTCG AGGCTGGCCC GGCCAGGGCC AGAGCTTATA TCGACAGGTT GCTGCATCTG 
TGGGGCCGGG AGACCCCAGC AAAGGGTAGC GAGGGAGAAC AGGGACCTCT AAGGCATGCA 
GCGTCCCCAT CTCCACAGAG GCAGGGTGGC TGGAGTACAG ACGGTGGAGG GCGACTGCTG 
GTCTTCGCCC CAGGGAGGGA GGACGAGGGA GGGCCAGCTC AGAGCAGGGG TGCCGGCAGG 
GGCGGGCCCC AGCAGCAGGG ATACATGCCC CTTGAGGGTC CCCAGCAGTC TGGCAGCCTT 
CCAGAGGAGG GCTCCAAGCC CTCAAACAGC TGTGTCCTCA GGGAGACCAT GGTGCAGGCT 
TCTCCCAGCT CAAAGGCCCA GCAGACACCC TCAGCTCAGG ACTATGGACG AGGCAACATC 
ATATCCCCAT CCAGGATGCT GGACAAGAGC CCCTCACCGG CCTCTGGGCA CTTTGCCCAC 
CCATCCTTTG CTGCCAGCCT GAAAATGGGT CCCCCCAAGA GCAAGGCTGA AAAAATCAAG 
AGAAGTCCCA TGGACAAGGT GCTGAGGTTT GCAAGGCAGC CGCTGCTTCT ACTGGACAGG 
CCTGAGGGAG CCCATGCAGC CCCCCAGCCA TCCCTGGAGT GGGACCCTGC CCACTGGCCC 
ACAGGGAGGG GCGGGCTCCA GCGGAGGCCA GCCCTGGCCT GGGAGGCACC CGGGCGCTCC 
TGTTCTGAGT CCACCCTCTA CCCCATGCCT GTCCTCGTCC CCTTGGCAGT GGCCCCGCAG 
GAGAGCCACC GGACCTCAGC CCAAGCCCTG TTCCCCTTTG AGGCGTCACT GCTCACCTCA 
GTGGCCAGGA GGAAGCATCG CCGCTGGCAG TCCACCGTGG AGATCTCGGC CCGGGCCCGC 
CTGGCCAGCT GTCCTGAGTC TAACCTGGGG CCCCCCAGGC CCGTGGCCAG GAGAGCAGGT 
GGCCCACTGG CCCGGGGCCG TCCCTCACTG GTCCGCCAGG ACGCCTACAC CAGGAGCGAC 
TCAGAGCCCT CCAAGCACTC GGCCGAGTGT GACCCGCGGT TCCCGTCAGT CATCCCGGAG 
ACCAGCGAGG GAGAGTCCAG TGACCACACC ACCAACCGAT TCGGAGACCG TGAGTCCAGC 
AGCAGCGACG AGGAGGGCGG CGCCCAGAGC AGGGACTGTG ACCTGGCACT GGGCTATGTG 
GCGGCCGGGC ATGCGGAGCT GGCCTGGACC CAGGAGGCCC CGGTCAGCTC GGGGCCACTC 
CTGTCCCCCG TGCCCAAGCT GTGCCGTATT AAGGCCTCCA AGGCCCTGAA GAAGAAGATC 
CGCAGGTTCC AGCCGACGGC CCTGAAGGTC ATGACCATGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999627.2
CDS	145..2469
Translation	MWTPGGPPGSAGWDRRRLGARLRAAFAGLQELQGLRATQQERVRGALALQPPPAPAAPCGPHGLHGPEQQLEAALAALQEQLSRLRQQDIGLKTHLDQLDLQISKLQLDVGTASGEALDSDSRPSSGFYEMSDGGSCSLSTSCASVCSDHISPSLGSLLPVAQAHKARPSMGDWRPRSVDETTVPAWRPQATEEGARPPGSVEDAGQPWGTFWPRPVSTGDLDRALPADTGLQKASADAELLGLLCQGVDIPLHVPDPKYRQDLVSQGGREVYPYPSPLHAVALQSPLFVLTKETPQRGGPSFPRESPRGPAGLNTIQTGPVLEAGPARARAYIDRLLHLWGRETPAKGSEGEQGPLRHAASPSPQRQGGWSTDGGGRLLVFAPGREDEGGPAQSRGAGRGGPQQQGYMPLEGPQQSGSLPEEGSKPSNSCVLRETMVQASPSSKAQQTPSAQDYGRGNIISPSRMLDKSPSPASGHFAHPSFAASLKMGPPKSKAEKIKRSPMDKVLRFARQPLLLLDRPEGAHAAPQPSLEWDPAHWPTGRGGLQRRPALAWEAPGRSCSESTLYPMPVLVPLAVAPQESHRTSAQALFPFEASLLTSVARRKHRRWQSTVEISARARLASCPESNLGPPRPVARRAGGPLARGRPSLVRQDAYTRSDSEPSKHSAECDPRFPSVIPETSEGESSDHTTNRFGDRESSSSDEEGGAQSRDCDLALGYVAAGHAELAWTQEAPVSSGPLLSPVPKLCRIKASKALKKKIRRFQPTALKVMTMV

Target ORF information:

RefSeq Version	NM_214462.5
Organism	Homo sapiens(human)
Definition	Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214462.5

ORF Insert Sequence:

ATGTGGACGC CGGGCGGACC CCCGGGGTCC GCGGGCTGGG ACCGCCGTAG GTTGGGCGCG 
AGGTTGCGCG CGGCGTTCGC GGGGCTGCAG GAGCTGCAGG GGCTGCGAGC CACGCAGCAG 
GAGCGGGTAC GGGGCGCCCT GGCCCTGCAG CCCCCGCCCG CGCCCGCCGC GCCCTGCGGC 
CCCCACGGCC TCCACGGCCC CGAGCAGCAG CTGGAGGCGG CGCTGGCCGC GCTGCAGGAG 
CAGCTGTCCC GGCTGAGACA ACAGGACATC GGCCTGAAGA CCCACCTGGA CCAGCTGGAC 
CTGCAGATTA GCAAGCTGCA GCTGGATGTG GGCACAGCCT CAGGGGAGGC CCTGGACAGC 
GACAGCAGGC CCAGCTCAGG CTTTTACGAG ATGAGCGACG GTGGATCCTG CTCCCTGTCC 
ACGTCCTGTG CCTCCGTCTG CAGTGACCAC ATCTCTCCCT CGCTGGGCAG TTTGTTGCCT 
GTGGCCCAGG CCCACAAGGC CAGGCCCAGC ATGGGGGACT GGAGGCCCCG GTCGGTTGAT 
GAGACTACTG TGCCAGCGTG GAGACCCCAG GCTACCGAGG AGGGCGCCAG GCCCCCAGGG 
AGCGTGGAGG ATGCAGGCCA GCCGTGGGGC ACATTCTGGC CCAGGCCTGT GTCTACAGGT 
GATCTTGACA GAGCCCTGCC GGCGGACACG GGGCTCCAGA AAGCCAGCGC GGACGCCGAG 
CTCCTCGGGC TCCTCTGCCA GGGGGTGGAT ATCCCGCTGC ACGTGCCGGA CCCCAAGTAT 
CGGCAGGACC TGGTGTCCCA GGGCGGCAGG GAGGTGTACC CGTACCCCAG CCCCCTGCAC 
GCCGTGGCTC TACAGAGCCC CCTGTTTGTC CTGACTAAGG AAACCCCACA GAGAGGTGGC 
CCCTCGTTCC CTAGGGAGAG CCCCAGGGGC CCCGCAGGTC TGAACACCAT CCAGACTGGG 
CCGGTCCTCG AGGCTGGCCC GGCCAGGGCC AGAGCTTATA TCGACAGGTT GCTGCATCTG 
TGGGGCCGGG AGACCCCAGC AAAGGGTAGC GAGGGAGAAC AGGGACCTCT AAGGCATGCA 
GCGTCCCCAT CTCCACAGAG GCAGGGTGGC TGGAGTACAG ACGGTGGAGG GCGACTGCTG 
GTCTTCGCCC CAGGGAGGGA GGACGAGGGA GGGCCAGCTC AGAGCAGGGG TGCCGGCAGG 
GGCGGGCCCC AGCAGCAGGG ATACATGCCC CTTGAGGGTC CCCAGCAGTC TGGCAGCCTT 
CCAGAGGAGG GCTCCAAGCC CTCAAACAGC TGTGTCCTCA GGGAGACCAT GGTGCAGGCT 
TCTCCCAGCT CAAAGGCCCA GCAGACACCC TCAGCTCAGG ACTATGGACG AGGCAACATC 
ATATCCCCAT CCAGGATGCT GGACAAGAGC CCCTCACCGG CCTCTGGGCA CTTTGCCCAC 
CCATCCTTTG CTGCCAGCCT GAAAATGGGT CCCCCCAAGA GCAAGGCTGA AAAAATCAAG 
AGAAGTCCCA TGGACAAGGT GCTGAGGTTT GCAAGGCAGC CGCTGCTTCT ACTGGACAGG 
CCTGAGGGAG CCCATGCAGC CCCCCAGCCA TCCCTGGAGT GGGACCCTGC CCACTGGCCC 
ACAGGGAGGG GCGGGCTCCA GCGGAGGCCA GCCCTGGCCT GGGAGGCACC CGGGCGCTCC 
TGTTCTGAGT CCACCCTCTA CCCCATGCCT GTCCTCGTCC CCTTGGCAGT GGCCCCGCAG 
GAGAGCCACC GGACCTCAGC CCAAGCCCTG TTCCCCTTTG AGGCGTCACT GCTCACCTCA 
GTGGCCAGGA GGAAGCATCG CCGCTGGCAG TCCACCGTGG AGATCTCGGC CCGGGCCCGC 
CTGGCCAGCT GTCCTGAGTC TAACCTGGGG CCCCCCAGGC CCGTGGCCAG GAGAGCAGGT 
GGCCCACTGG CCCGGGGCCG TCCCTCACTG GTCCGCCAGG ACGCCTACAC CAGGAGCGAC 
TCAGAGCCCT CCAAGCACTC GGCCGAGTGT GACCCGCGGT TCCCGTCAGT CATCCCGGAG 
ACCAGCGAGG GAGAGTCCAG TGACCACACC ACCAACCGAT TCGGAGACCG TGAGTCCAGC 
AGCAGCGACG AGGAGGGCGG CGCCCAGAGC AGGGACTGTG ACCTGGCACT GGGCTATGTG 
GCGGCCGGGC ATGCGGAGCT GGCCTGGACC CAGGAGGCCC CGGTCAGCTC GGGGCCACTC 
CTGTCCCCCG TGCCCAAGCT GTGCCGTATT AAGGCCTCCA AGGCCCTGAA GAAGAAGATC 
CGCAGGTTCC AGCCGACGGC CCTGAAGGTC ATGACCATGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DACT2 ( NM_214462.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_999627.2 Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09961
Clone ID Related Accession (Same CDS sequence)	NM_214462.5 , NM_214462.4
Accession Version	NM_214462.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	dapper homolog 2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC092498.1, BC111790.1, AF318336.1 and BX377941.2. On Nov 2, 2013 this sequence version replaced NM_214462.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF318336.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGACGC CGGGCGGACC CCCGGGGTCC GCGGGCTGGG ACCGCCGTAG GTTGGGCGCG 
AGGTTGCGCG CGGCGTTCGC GGGGCTGCAG GAGCTGCAGG GGCTGCGAGC CACGCAGCAG 
GAGCGGGTAC GGGGCGCCCT GGCCCTGCAG CCCCCGCCCG CGCCCGCCGC GCCCTGCGGC 
CCCCACGGCC TCCACGGCCC CGAGCAGCAG CTGGAGGCGG CGCTGGCCGC GCTGCAGGAG 
CAGCTGTCCC GGCTGAGACA ACAGGACATC GGCCTGAAGA CCCACCTGGA CCAGCTGGAC 
CTGCAGATTA GCAAGCTGCA GCTGGATGTG GGCACAGCCT CAGGGGAGGC CCTGGACAGC 
GACAGCAGGC CCAGCTCAGG CTTTTACGAG ATGAGCGACG GTGGATCCTG CTCCCTGTCC 
ACGTCCTGTG CCTCCGTCTG CAGTGACCAC ATCTCTCCCT CGCTGGGCAG TTTGTTGCCT 
GTGGCCCAGG CCCACAAGGC CAGGCCCAGC ATGGGGGACT GGAGGCCCCG GTCGGTTGAT 
GAGACTACTG TGCCAGCGTG GAGACCCCAG GCTACCGAGG AGGGCGCCAG GCCCCCAGGG 
AGCGTGGAGG ATGCAGGCCA GCCGTGGGGC ACATTCTGGC CCAGGCCTGT GTCTACAGGT 
GATCTTGACA GAGCCCTGCC GGCGGACACG GGGCTCCAGA AAGCCAGCGC GGACGCCGAG 
CTCCTCGGGC TCCTCTGCCA GGGGGTGGAT ATCCCGCTGC ACGTGCCGGA CCCCAAGTAT 
CGGCAGGACC TGGTGTCCCA GGGCGGCAGG GAGGTGTACC CGTACCCCAG CCCCCTGCAC 
GCCGTGGCTC TACAGAGCCC CCTGTTTGTC CTGACTAAGG AAACCCCACA GAGAGGTGGC 
CCCTCGTTCC CTAGGGAGAG CCCCAGGGGC CCCGCAGGTC TGAACACCAT CCAGACTGGG 
CCGGTCCTCG AGGCTGGCCC GGCCAGGGCC AGAGCTTATA TCGACAGGTT GCTGCATCTG 
TGGGGCCGGG AGACCCCAGC AAAGGGTAGC GAGGGAGAAC AGGGACCTCT AAGGCATGCA 
GCGTCCCCAT CTCCACAGAG GCAGGGTGGC TGGAGTACAG ACGGTGGAGG GCGACTGCTG 
GTCTTCGCCC CAGGGAGGGA GGACGAGGGA GGGCCAGCTC AGAGCAGGGG TGCCGGCAGG 
GGCGGGCCCC AGCAGCAGGG ATACATGCCC CTTGAGGGTC CCCAGCAGTC TGGCAGCCTT 
CCAGAGGAGG GCTCCAAGCC CTCAAACAGC TGTGTCCTCA GGGAGACCAT GGTGCAGGCT 
TCTCCCAGCT CAAAGGCCCA GCAGACACCC TCAGCTCAGG ACTATGGACG AGGCAACATC 
ATATCCCCAT CCAGGATGCT GGACAAGAGC CCCTCACCGG CCTCTGGGCA CTTTGCCCAC 
CCATCCTTTG CTGCCAGCCT GAAAATGGGT CCCCCCAAGA GCAAGGCTGA AAAAATCAAG 
AGAAGTCCCA TGGACAAGGT GCTGAGGTTT GCAAGGCAGC CGCTGCTTCT ACTGGACAGG 
CCTGAGGGAG CCCATGCAGC CCCCCAGCCA TCCCTGGAGT GGGACCCTGC CCACTGGCCC 
ACAGGGAGGG GCGGGCTCCA GCGGAGGCCA GCCCTGGCCT GGGAGGCACC CGGGCGCTCC 
TGTTCTGAGT CCACCCTCTA CCCCATGCCT GTCCTCGTCC CCTTGGCAGT GGCCCCGCAG 
GAGAGCCACC GGACCTCAGC CCAAGCCCTG TTCCCCTTTG AGGCGTCACT GCTCACCTCA 
GTGGCCAGGA GGAAGCATCG CCGCTGGCAG TCCACCGTGG AGATCTCGGC CCGGGCCCGC 
CTGGCCAGCT GTCCTGAGTC TAACCTGGGG CCCCCCAGGC CCGTGGCCAG GAGAGCAGGT 
GGCCCACTGG CCCGGGGCCG TCCCTCACTG GTCCGCCAGG ACGCCTACAC CAGGAGCGAC 
TCAGAGCCCT CCAAGCACTC GGCCGAGTGT GACCCGCGGT TCCCGTCAGT CATCCCGGAG 
ACCAGCGAGG GAGAGTCCAG TGACCACACC ACCAACCGAT TCGGAGACCG TGAGTCCAGC 
AGCAGCGACG AGGAGGGCGG CGCCCAGAGC AGGGACTGTG ACCTGGCACT GGGCTATGTG 
GCGGCCGGGC ATGCGGAGCT GGCCTGGACC CAGGAGGCCC CGGTCAGCTC GGGGCCACTC 
CTGTCCCCCG TGCCCAAGCT GTGCCGTATT AAGGCCTCCA AGGCCCTGAA GAAGAAGATC 
CGCAGGTTCC AGCCGACGGC CCTGAAGGTC ATGACCATGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999627.2
CDS	122..2446
Translation	MWTPGGPPGSAGWDRRRLGARLRAAFAGLQELQGLRATQQERVRGALALQPPPAPAAPCGPHGLHGPEQQLEAALAALQEQLSRLRQQDIGLKTHLDQLDLQISKLQLDVGTASGEALDSDSRPSSGFYEMSDGGSCSLSTSCASVCSDHISPSLGSLLPVAQAHKARPSMGDWRPRSVDETTVPAWRPQATEEGARPPGSVEDAGQPWGTFWPRPVSTGDLDRALPADTGLQKASADAELLGLLCQGVDIPLHVPDPKYRQDLVSQGGREVYPYPSPLHAVALQSPLFVLTKETPQRGGPSFPRESPRGPAGLNTIQTGPVLEAGPARARAYIDRLLHLWGRETPAKGSEGEQGPLRHAASPSPQRQGGWSTDGGGRLLVFAPGREDEGGPAQSRGAGRGGPQQQGYMPLEGPQQSGSLPEEGSKPSNSCVLRETMVQASPSSKAQQTPSAQDYGRGNIISPSRMLDKSPSPASGHFAHPSFAASLKMGPPKSKAEKIKRSPMDKVLRFARQPLLLLDRPEGAHAAPQPSLEWDPAHWPTGRGGLQRRPALAWEAPGRSCSESTLYPMPVLVPLAVAPQESHRTSAQALFPFEASLLTSVARRKHRRWQSTVEISARARLASCPESNLGPPRPVARRAGGPLARGRPSLVRQDAYTRSDSEPSKHSAECDPRFPSVIPETSEGESSDHTTNRFGDRESSSSDEEGGAQSRDCDLALGYVAAGHAELAWTQEAPVSSGPLLSPVPKLCRIKASKALKKKIRRFQPTALKVMTMV

Target ORF information:

RefSeq Version	NM_214462.4
Organism	Homo sapiens(human)
Definition	Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214462.4

ORF Insert Sequence:

ATGTGGACGC CGGGCGGACC CCCGGGGTCC GCGGGCTGGG ACCGCCGTAG GTTGGGCGCG 
AGGTTGCGCG CGGCGTTCGC GGGGCTGCAG GAGCTGCAGG GGCTGCGAGC CACGCAGCAG 
GAGCGGGTAC GGGGCGCCCT GGCCCTGCAG CCCCCGCCCG CGCCCGCCGC GCCCTGCGGC 
CCCCACGGCC TCCACGGCCC CGAGCAGCAG CTGGAGGCGG CGCTGGCCGC GCTGCAGGAG 
CAGCTGTCCC GGCTGAGACA ACAGGACATC GGCCTGAAGA CCCACCTGGA CCAGCTGGAC 
CTGCAGATTA GCAAGCTGCA GCTGGATGTG GGCACAGCCT CAGGGGAGGC CCTGGACAGC 
GACAGCAGGC CCAGCTCAGG CTTTTACGAG ATGAGCGACG GTGGATCCTG CTCCCTGTCC 
ACGTCCTGTG CCTCCGTCTG CAGTGACCAC ATCTCTCCCT CGCTGGGCAG TTTGTTGCCT 
GTGGCCCAGG CCCACAAGGC CAGGCCCAGC ATGGGGGACT GGAGGCCCCG GTCGGTTGAT 
GAGACTACTG TGCCAGCGTG GAGACCCCAG GCTACCGAGG AGGGCGCCAG GCCCCCAGGG 
AGCGTGGAGG ATGCAGGCCA GCCGTGGGGC ACATTCTGGC CCAGGCCTGT GTCTACAGGT 
GATCTTGACA GAGCCCTGCC GGCGGACACG GGGCTCCAGA AAGCCAGCGC GGACGCCGAG 
CTCCTCGGGC TCCTCTGCCA GGGGGTGGAT ATCCCGCTGC ACGTGCCGGA CCCCAAGTAT 
CGGCAGGACC TGGTGTCCCA GGGCGGCAGG GAGGTGTACC CGTACCCCAG CCCCCTGCAC 
GCCGTGGCTC TACAGAGCCC CCTGTTTGTC CTGACTAAGG AAACCCCACA GAGAGGTGGC 
CCCTCGTTCC CTAGGGAGAG CCCCAGGGGC CCCGCAGGTC TGAACACCAT CCAGACTGGG 
CCGGTCCTCG AGGCTGGCCC GGCCAGGGCC AGAGCTTATA TCGACAGGTT GCTGCATCTG 
TGGGGCCGGG AGACCCCAGC AAAGGGTAGC GAGGGAGAAC AGGGACCTCT AAGGCATGCA 
GCGTCCCCAT CTCCACAGAG GCAGGGTGGC TGGAGTACAG ACGGTGGAGG GCGACTGCTG 
GTCTTCGCCC CAGGGAGGGA GGACGAGGGA GGGCCAGCTC AGAGCAGGGG TGCCGGCAGG 
GGCGGGCCCC AGCAGCAGGG ATACATGCCC CTTGAGGGTC CCCAGCAGTC TGGCAGCCTT 
CCAGAGGAGG GCTCCAAGCC CTCAAACAGC TGTGTCCTCA GGGAGACCAT GGTGCAGGCT 
TCTCCCAGCT CAAAGGCCCA GCAGACACCC TCAGCTCAGG ACTATGGACG AGGCAACATC 
ATATCCCCAT CCAGGATGCT GGACAAGAGC CCCTCACCGG CCTCTGGGCA CTTTGCCCAC 
CCATCCTTTG CTGCCAGCCT GAAAATGGGT CCCCCCAAGA GCAAGGCTGA AAAAATCAAG 
AGAAGTCCCA TGGACAAGGT GCTGAGGTTT GCAAGGCAGC CGCTGCTTCT ACTGGACAGG 
CCTGAGGGAG CCCATGCAGC CCCCCAGCCA TCCCTGGAGT GGGACCCTGC CCACTGGCCC 
ACAGGGAGGG GCGGGCTCCA GCGGAGGCCA GCCCTGGCCT GGGAGGCACC CGGGCGCTCC 
TGTTCTGAGT CCACCCTCTA CCCCATGCCT GTCCTCGTCC CCTTGGCAGT GGCCCCGCAG 
GAGAGCCACC GGACCTCAGC CCAAGCCCTG TTCCCCTTTG AGGCGTCACT GCTCACCTCA 
GTGGCCAGGA GGAAGCATCG CCGCTGGCAG TCCACCGTGG AGATCTCGGC CCGGGCCCGC 
CTGGCCAGCT GTCCTGAGTC TAACCTGGGG CCCCCCAGGC CCGTGGCCAG GAGAGCAGGT 
GGCCCACTGG CCCGGGGCCG TCCCTCACTG GTCCGCCAGG ACGCCTACAC CAGGAGCGAC 
TCAGAGCCCT CCAAGCACTC GGCCGAGTGT GACCCGCGGT TCCCGTCAGT CATCCCGGAG 
ACCAGCGAGG GAGAGTCCAG TGACCACACC ACCAACCGAT TCGGAGACCG TGAGTCCAGC 
AGCAGCGACG AGGAGGGCGG CGCCCAGAGC AGGGACTGTG ACCTGGCACT GGGCTATGTG 
GCGGCCGGGC ATGCGGAGCT GGCCTGGACC CAGGAGGCCC CGGTCAGCTC GGGGCCACTC 
CTGTCCCCCG TGCCCAAGCT GTGCCGTATT AAGGCCTCCA AGGCCCTGAA GAAGAAGATC 
CGCAGGTTCC AGCCGACGGC CCTGAAGGTC ATGACCATGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DACT2 ( NM_001286350.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273279.1 Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 2, mRNA.

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CloneID	OHu14407
Clone ID Related Accession (Same CDS sequence)	NM_001286350.1 , NM_001286350.2
Accession Version	NM_001286350.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	dapper homolog 2 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC092498.1, BC111790.1, BX377941.2 and AF318336.1. Transcript Variant: This variant (2) lacks a portion of the 5' UTR and 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC111790.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGACT GGAGGCCCCG GTCGGTTGAT GAGACTACTG TGCCAGCGTG GAGACCCCAG 
GCTACCGAGG AGGGCGCCAG GCCCCCAGGG AGCGTGGAGG ATGCAGGCCA GCCGTGGGGC 
ACATTCTGGC CCAGGCCTGT GTCTACAGGT GATCTTGACA GAGCCCTGCC GGCGGACACG 
GGGCTCCAGA AAGCCAGCGC GGACGCCGAG CTCCTCGGGC TCCTCTGCCA GGGGGTGGAT 
ATCCCGCTGC ACGTGCCGGA CCCCAAGTAT CGGCAGGACC TGGTGTCCCA GGGCGGCAGG 
GAGGTGTACC CGTACCCCAG CCCCCTGCAC GCCGTGGCTC TACAGAGCCC CCTGTTTGTC 
CTGACTAAGG AAACCCCACA GAGAGGTGGC CCCTCGTTCC CTAGGGAGAG CCCCAGGGGC 
CCCGCAGGTC TGAACACCAT CCAGACTGGG CCGGTCCTCG AGGCTGGCCC GGCCAGGGCC 
AGAGCTTATA TCGACAGGTT GCTGCATCTG TGGGGCCGGG AGACCCCAGC AAAGGGTAGC 
GAGGGAGAAC AGGGACCTCT AAGGCATGCA GCGTCCCCAT CTCCACAGAG GCAGGGTGGC 
TGGAGTACAG ACGGTGGAGG GCGACTGCTG GTCTTCGCCC CAGGGAGGGA GGACGAGGGA 
GGGCCAGCTC AGAGCAGGGG TGCCGGCAGG GGCGGGCCCC AGCAGCAGGG ATACATGCCC 
CTTGAGGGTC CCCAGCAGTC TGGCAGCCTT CCAGAGGAGG GCTCCAAGCC CTCAAACAGC 
TGTGTCCTCA GGGAGACCAT GGTGCAGGCT TCTCCCAGCT CAAAGGCCCA GCAGACACCC 
TCAGCTCAGG ACTATGGACG AGGCAACATC ATATCCCCAT CCAGGATGCT GGACAAGAGC 
CCCTCACCGG CCTCTGGGCA CTTTGCCCAC CCATCCTTTG CTGCCAGCCT GAAAATGGGT 
CCCCCCAAGA GCAAGGCTGA AAAAATCAAG AGAAGTCCCA TGGACAAGGT GCTGAGGTTT 
GCAAGGCAGC CGCTGCTTCT ACTGGACAGG CCTGAGGGAG CCCATGCAGC CCCCCAGCCA 
TCCCTGGAGT GGGACCCTGC CCACTGGCCC ACAGGGAGGG GCGGGCTCCA GCGGAGGCCA 
GCCCTGGCCT GGGAGGCACC CGGGCGCTCC TGTTCTGAGT CCACCCTCTA CCCCATGCCT 
GTCCTCGTCC CCTTGGCAGT GGCCCCGCAG GAGAGCCACC GGACCTCAGC CCAAGCCCTG 
TTCCCCTTTG AGGCGTCACT GCTCACCTCA GTGGCCAGGA GGAAGCATCG CCGCTGGCAG 
TCCACCGTGG AGATCTCGGC CCGGGCCCGC CTGGCCAGCT GTCCTGAGTC TAACCTGGGG 
CCCCCCAGGC CCGTGGCCAG GAGAGCAGGT GGCCCACTGG CCCGGGGCCG TCCCTCACTG 
GTCCGCCAGG ACGCCTACAC CAGGAGCGAC TCAGAGCCCT CCAAGCACTC GGCCGAGTGT 
GACCCGCGGT TCCCGTCAGT CATCCCGGAG ACCAGCGAGG GAGAGTCCAG TGACCACACC 
ACCAACCGAT TCGGAGACCG TGAGTCCAGC AGCAGCGACG AGGAGGGCGG CGCCCAGAGC 
AGGGACTGTG ACCTGGCACT GGGCTATGTG GCGGCCGGGC ATGCGGAGCT GGCCTGGACC 
CAGGAGGCCC CGGTCAGCTC GGGGCCACTC CTGTCCCCCG TGCCCAAGCT GTGCCGTATT 
AAGGCCTCCA AGGCCCTGAA GAAGAAGATC CGCAGGTTCC AGCCGACGGC CCTGAAGGTC 
ATGACCATGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273279.1
CDS	109..1923
Translation	MGDWRPRSVDETTVPAWRPQATEEGARPPGSVEDAGQPWGTFWPRPVSTGDLDRALPADTGLQKASADAELLGLLCQGVDIPLHVPDPKYRQDLVSQGGREVYPYPSPLHAVALQSPLFVLTKETPQRGGPSFPRESPRGPAGLNTIQTGPVLEAGPARARAYIDRLLHLWGRETPAKGSEGEQGPLRHAASPSPQRQGGWSTDGGGRLLVFAPGREDEGGPAQSRGAGRGGPQQQGYMPLEGPQQSGSLPEEGSKPSNSCVLRETMVQASPSSKAQQTPSAQDYGRGNIISPSRMLDKSPSPASGHFAHPSFAASLKMGPPKSKAEKIKRSPMDKVLRFARQPLLLLDRPEGAHAAPQPSLEWDPAHWPTGRGGLQRRPALAWEAPGRSCSESTLYPMPVLVPLAVAPQESHRTSAQALFPFEASLLTSVARRKHRRWQSTVEISARARLASCPESNLGPPRPVARRAGGPLARGRPSLVRQDAYTRSDSEPSKHSAECDPRFPSVIPETSEGESSDHTTNRFGDRESSSSDEEGGAQSRDCDLALGYVAAGHAELAWTQEAPVSSGPLLSPVPKLCRIKASKALKKKIRRFQPTALKVMTMV

Target ORF information:

RefSeq Version	NM_001286350.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286350.1

ORF Insert Sequence:

ATGGGGGACT GGAGGCCCCG GTCGGTTGAT GAGACTACTG TGCCAGCGTG GAGACCCCAG 
GCTACCGAGG AGGGCGCCAG GCCCCCAGGG AGCGTGGAGG ATGCAGGCCA GCCGTGGGGC 
ACATTCTGGC CCAGGCCTGT GTCTACAGGT GATCTTGACA GAGCCCTGCC GGCGGACACG 
GGGCTCCAGA AAGCCAGCGC GGACGCCGAG CTCCTCGGGC TCCTCTGCCA GGGGGTGGAT 
ATCCCGCTGC ACGTGCCGGA CCCCAAGTAT CGGCAGGACC TGGTGTCCCA GGGCGGCAGG 
GAGGTGTACC CGTACCCCAG CCCCCTGCAC GCCGTGGCTC TACAGAGCCC CCTGTTTGTC 
CTGACTAAGG AAACCCCACA GAGAGGTGGC CCCTCGTTCC CTAGGGAGAG CCCCAGGGGC 
CCCGCAGGTC TGAACACCAT CCAGACTGGG CCGGTCCTCG AGGCTGGCCC GGCCAGGGCC 
AGAGCTTATA TCGACAGGTT GCTGCATCTG TGGGGCCGGG AGACCCCAGC AAAGGGTAGC 
GAGGGAGAAC AGGGACCTCT AAGGCATGCA GCGTCCCCAT CTCCACAGAG GCAGGGTGGC 
TGGAGTACAG ACGGTGGAGG GCGACTGCTG GTCTTCGCCC CAGGGAGGGA GGACGAGGGA 
GGGCCAGCTC AGAGCAGGGG TGCCGGCAGG GGCGGGCCCC AGCAGCAGGG ATACATGCCC 
CTTGAGGGTC CCCAGCAGTC TGGCAGCCTT CCAGAGGAGG GCTCCAAGCC CTCAAACAGC 
TGTGTCCTCA GGGAGACCAT GGTGCAGGCT TCTCCCAGCT CAAAGGCCCA GCAGACACCC 
TCAGCTCAGG ACTATGGACG AGGCAACATC ATATCCCCAT CCAGGATGCT GGACAAGAGC 
CCCTCACCGG CCTCTGGGCA CTTTGCCCAC CCATCCTTTG CTGCCAGCCT GAAAATGGGT 
CCCCCCAAGA GCAAGGCTGA AAAAATCAAG AGAAGTCCCA TGGACAAGGT GCTGAGGTTT 
GCAAGGCAGC CGCTGCTTCT ACTGGACAGG CCTGAGGGAG CCCATGCAGC CCCCCAGCCA 
TCCCTGGAGT GGGACCCTGC CCACTGGCCC ACAGGGAGGG GCGGGCTCCA GCGGAGGCCA 
GCCCTGGCCT GGGAGGCACC CGGGCGCTCC TGTTCTGAGT CCACCCTCTA CCCCATGCCT 
GTCCTCGTCC CCTTGGCAGT GGCCCCGCAG GAGAGCCACC GGACCTCAGC CCAAGCCCTG 
TTCCCCTTTG AGGCGTCACT GCTCACCTCA GTGGCCAGGA GGAAGCATCG CCGCTGGCAG 
TCCACCGTGG AGATCTCGGC CCGGGCCCGC CTGGCCAGCT GTCCTGAGTC TAACCTGGGG 
CCCCCCAGGC CCGTGGCCAG GAGAGCAGGT GGCCCACTGG CCCGGGGCCG TCCCTCACTG 
GTCCGCCAGG ACGCCTACAC CAGGAGCGAC TCAGAGCCCT CCAAGCACTC GGCCGAGTGT 
GACCCGCGGT TCCCGTCAGT CATCCCGGAG ACCAGCGAGG GAGAGTCCAG TGACCACACC 
ACCAACCGAT TCGGAGACCG TGAGTCCAGC AGCAGCGACG AGGAGGGCGG CGCCCAGAGC 
AGGGACTGTG ACCTGGCACT GGGCTATGTG GCGGCCGGGC ATGCGGAGCT GGCCTGGACC 
CAGGAGGCCC CGGTCAGCTC GGGGCCACTC CTGTCCCCCG TGCCCAAGCT GTGCCGTATT 
AAGGCCTCCA AGGCCCTGAA GAAGAAGATC CGCAGGTTCC AGCCGACGGC CCTGAAGGTC 
ATGACCATGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DACT2 ( NM_001286350.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273279.1 Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 2, mRNA.

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CloneID	OHu14407
Clone ID Related Accession (Same CDS sequence)	NM_001286350.1 , NM_001286350.2
Accession Version	NM_001286350.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Homo sapiens(human)
Product	dapper homolog 2 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL606970.12, BC092498.1, BC111790.1, BX377941.2 and AF318336.1. On May 31, 2019 this sequence version replaced NM_001286350.1. Transcript Variant: This variant (2) lacks a portion of the 5' UTR and 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC111790.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGACT GGAGGCCCCG GTCGGTTGAT GAGACTACTG TGCCAGCGTG GAGACCCCAG 
GCTACCGAGG AGGGCGCCAG GCCCCCAGGG AGCGTGGAGG ATGCAGGCCA GCCGTGGGGC 
ACATTCTGGC CCAGGCCTGT GTCTACAGGT GATCTTGACA GAGCCCTGCC GGCGGACACG 
GGGCTCCAGA AAGCCAGCGC GGACGCCGAG CTCCTCGGGC TCCTCTGCCA GGGGGTGGAT 
ATCCCGCTGC ACGTGCCGGA CCCCAAGTAT CGGCAGGACC TGGTGTCCCA GGGCGGCAGG 
GAGGTGTACC CGTACCCCAG CCCCCTGCAC GCCGTGGCTC TACAGAGCCC CCTGTTTGTC 
CTGACTAAGG AAACCCCACA GAGAGGTGGC CCCTCGTTCC CTAGGGAGAG CCCCAGGGGC 
CCCGCAGGTC TGAACACCAT CCAGACTGGG CCGGTCCTCG AGGCTGGCCC GGCCAGGGCC 
AGAGCTTATA TCGACAGGTT GCTGCATCTG TGGGGCCGGG AGACCCCAGC AAAGGGTAGC 
GAGGGAGAAC AGGGACCTCT AAGGCATGCA GCGTCCCCAT CTCCACAGAG GCAGGGTGGC 
TGGAGTACAG ACGGTGGAGG GCGACTGCTG GTCTTCGCCC CAGGGAGGGA GGACGAGGGA 
GGGCCAGCTC AGAGCAGGGG TGCCGGCAGG GGCGGGCCCC AGCAGCAGGG ATACATGCCC 
CTTGAGGGTC CCCAGCAGTC TGGCAGCCTT CCAGAGGAGG GCTCCAAGCC CTCAAACAGC 
TGTGTCCTCA GGGAGACCAT GGTGCAGGCT TCTCCCAGCT CAAAGGCCCA GCAGACACCC 
TCAGCTCAGG ACTATGGACG AGGCAACATC ATATCCCCAT CCAGGATGCT GGACAAGAGC 
CCCTCACCGG CCTCTGGGCA CTTTGCCCAC CCATCCTTTG CTGCCAGCCT GAAAATGGGT 
CCCCCCAAGA GCAAGGCTGA AAAAATCAAG AGAAGTCCCA TGGACAAGGT GCTGAGGTTT 
GCAAGGCAGC CGCTGCTTCT ACTGGACAGG CCTGAGGGAG CCCATGCAGC CCCCCAGCCA 
TCCCTGGAGT GGGACCCTGC CCACTGGCCC ACAGGGAGGG GCGGGCTCCA GCGGAGGCCA 
GCCCTGGCCT GGGAGGCACC CGGGCGCTCC TGTTCTGAGT CCACCCTCTA CCCCATGCCT 
GTCCTCGTCC CCTTGGCAGT GGCCCCGCAG GAGAGCCACC GGACCTCAGC CCAAGCCCTG 
TTCCCCTTTG AGGCGTCACT GCTCACCTCA GTGGCCAGGA GGAAGCATCG CCGCTGGCAG 
TCCACCGTGG AGATCTCGGC CCGGGCCCGC CTGGCCAGCT GTCCTGAGTC TAACCTGGGG 
CCCCCCAGGC CCGTGGCCAG GAGAGCAGGT GGCCCACTGG CCCGGGGCCG TCCCTCACTG 
GTCCGCCAGG ACGCCTACAC CAGGAGCGAC TCAGAGCCCT CCAAGCACTC GGCCGAGTGT 
GACCCGCGGT TCCCGTCAGT CATCCCGGAG ACCAGCGAGG GAGAGTCCAG TGACCACACC 
ACCAACCGAT TCGGAGACCG TGAGTCCAGC AGCAGCGACG AGGAGGGCGG CGCCCAGAGC 
AGGGACTGTG ACCTGGCACT GGGCTATGTG GCGGCCGGGC ATGCGGAGCT GGCCTGGACC 
CAGGAGGCCC CGGTCAGCTC GGGGCCACTC CTGTCCCCCG TGCCCAAGCT GTGCCGTATT 
AAGGCCTCCA AGGCCCTGAA GAAGAAGATC CGCAGGTTCC AGCCGACGGC CCTGAAGGTC 
ATGACCATGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273279.1
CDS	132..1946
Translation	MGDWRPRSVDETTVPAWRPQATEEGARPPGSVEDAGQPWGTFWPRPVSTGDLDRALPADTGLQKASADAELLGLLCQGVDIPLHVPDPKYRQDLVSQGGREVYPYPSPLHAVALQSPLFVLTKETPQRGGPSFPRESPRGPAGLNTIQTGPVLEAGPARARAYIDRLLHLWGRETPAKGSEGEQGPLRHAASPSPQRQGGWSTDGGGRLLVFAPGREDEGGPAQSRGAGRGGPQQQGYMPLEGPQQSGSLPEEGSKPSNSCVLRETMVQASPSSKAQQTPSAQDYGRGNIISPSRMLDKSPSPASGHFAHPSFAASLKMGPPKSKAEKIKRSPMDKVLRFARQPLLLLDRPEGAHAAPQPSLEWDPAHWPTGRGGLQRRPALAWEAPGRSCSESTLYPMPVLVPLAVAPQESHRTSAQALFPFEASLLTSVARRKHRRWQSTVEISARARLASCPESNLGPPRPVARRAGGPLARGRPSLVRQDAYTRSDSEPSKHSAECDPRFPSVIPETSEGESSDHTTNRFGDRESSSSDEEGGAQSRDCDLALGYVAAGHAELAWTQEAPVSSGPLLSPVPKLCRIKASKALKKKIRRFQPTALKVMTMV

Target ORF information:

RefSeq Version	NM_001286350.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286350.2

ORF Insert Sequence:

ATGGGGGACT GGAGGCCCCG GTCGGTTGAT GAGACTACTG TGCCAGCGTG GAGACCCCAG 
GCTACCGAGG AGGGCGCCAG GCCCCCAGGG AGCGTGGAGG ATGCAGGCCA GCCGTGGGGC 
ACATTCTGGC CCAGGCCTGT GTCTACAGGT GATCTTGACA GAGCCCTGCC GGCGGACACG 
GGGCTCCAGA AAGCCAGCGC GGACGCCGAG CTCCTCGGGC TCCTCTGCCA GGGGGTGGAT 
ATCCCGCTGC ACGTGCCGGA CCCCAAGTAT CGGCAGGACC TGGTGTCCCA GGGCGGCAGG 
GAGGTGTACC CGTACCCCAG CCCCCTGCAC GCCGTGGCTC TACAGAGCCC CCTGTTTGTC 
CTGACTAAGG AAACCCCACA GAGAGGTGGC CCCTCGTTCC CTAGGGAGAG CCCCAGGGGC 
CCCGCAGGTC TGAACACCAT CCAGACTGGG CCGGTCCTCG AGGCTGGCCC GGCCAGGGCC 
AGAGCTTATA TCGACAGGTT GCTGCATCTG TGGGGCCGGG AGACCCCAGC AAAGGGTAGC 
GAGGGAGAAC AGGGACCTCT AAGGCATGCA GCGTCCCCAT CTCCACAGAG GCAGGGTGGC 
TGGAGTACAG ACGGTGGAGG GCGACTGCTG GTCTTCGCCC CAGGGAGGGA GGACGAGGGA 
GGGCCAGCTC AGAGCAGGGG TGCCGGCAGG GGCGGGCCCC AGCAGCAGGG ATACATGCCC 
CTTGAGGGTC CCCAGCAGTC TGGCAGCCTT CCAGAGGAGG GCTCCAAGCC CTCAAACAGC 
TGTGTCCTCA GGGAGACCAT GGTGCAGGCT TCTCCCAGCT CAAAGGCCCA GCAGACACCC 
TCAGCTCAGG ACTATGGACG AGGCAACATC ATATCCCCAT CCAGGATGCT GGACAAGAGC 
CCCTCACCGG CCTCTGGGCA CTTTGCCCAC CCATCCTTTG CTGCCAGCCT GAAAATGGGT 
CCCCCCAAGA GCAAGGCTGA AAAAATCAAG AGAAGTCCCA TGGACAAGGT GCTGAGGTTT 
GCAAGGCAGC CGCTGCTTCT ACTGGACAGG CCTGAGGGAG CCCATGCAGC CCCCCAGCCA 
TCCCTGGAGT GGGACCCTGC CCACTGGCCC ACAGGGAGGG GCGGGCTCCA GCGGAGGCCA 
GCCCTGGCCT GGGAGGCACC CGGGCGCTCC TGTTCTGAGT CCACCCTCTA CCCCATGCCT 
GTCCTCGTCC CCTTGGCAGT GGCCCCGCAG GAGAGCCACC GGACCTCAGC CCAAGCCCTG 
TTCCCCTTTG AGGCGTCACT GCTCACCTCA GTGGCCAGGA GGAAGCATCG CCGCTGGCAG 
TCCACCGTGG AGATCTCGGC CCGGGCCCGC CTGGCCAGCT GTCCTGAGTC TAACCTGGGG 
CCCCCCAGGC CCGTGGCCAG GAGAGCAGGT GGCCCACTGG CCCGGGGCCG TCCCTCACTG 
GTCCGCCAGG ACGCCTACAC CAGGAGCGAC TCAGAGCCCT CCAAGCACTC GGCCGAGTGT 
GACCCGCGGT TCCCGTCAGT CATCCCGGAG ACCAGCGAGG GAGAGTCCAG TGACCACACC 
ACCAACCGAT TCGGAGACCG TGAGTCCAGC AGCAGCGACG AGGAGGGCGG CGCCCAGAGC 
AGGGACTGTG ACCTGGCACT GGGCTATGTG GCGGCCGGGC ATGCGGAGCT GGCCTGGACC 
CAGGAGGCCC CGGTCAGCTC GGGGCCACTC CTGTCCCCCG TGCCCAAGCT GTGCCGTATT 
AAGGCCTCCA AGGCCCTGAA GAAGAAGATC CGCAGGTTCC AGCCGACGGC CCTGAAGGTC 
ATGACCATGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DACT2 ( XM_011535507.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533809.1 Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu77362
Clone ID Related Accession (Same CDS sequence)	XM_011535507.2
Accession Version	XM_011535507.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dapper homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535507.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGACG GTGGATCCTG CTCCCTGTCC ACGTCCTGTG CCTCCGTCTG CAGTGACCAC 
ATCTCTCCCT CGCTGGGCAG TTTGTTGCCT GTGGCCCAGG CCCACAAGGC CAGGCCCAGC 
ATGGGGGACT GGAGGCCCCG GTCGGTTGAT GAGACTACTG TGCCAGCGTG GAGACCCCAG 
GCTACCGAGG AGGGCGCCAG GCCCCCAGGG AGCGTGGAGG ATGCAGGCCA GCCGTGGGGC 
ACATTCTGGC CCAGGCCTGT GTCTACAGGT GATCTTGACA GAGCCCTGCC GGCGGACACG 
GGGCTCCAGA AAGCCAGCGC GGACGCCGAG CTCCTCGGGC TCCTCTGCCA GGGGGTGGAT 
ATCCCGCTGC ACGTGCCGGA CCCCAAGTAT CGGCAGGACC TGGTGTCCCA GGGCGGCAGG 
GAGGTGTACC CGTACCCCAG CCCCCTGCAC GCCGTGGCTC TACAGAGCCC CCTGTTTGTC 
CTGACTAAGG AAACCCCACA GAGAGGTGGC CCCTCGTTCC CTAGGGAGAG CCCCAGGGGC 
CCCGCAGGTC TGAACACCAT CCAGACTGGG CCGGTCCTCG AGGCTGGCCC GGCCAGGGCC 
AGAGCTTATA TCGACAGGTT GCTGCATCTG TGGGGCCGGG AGACCCCAGC AAAGGGTAGC 
GAGGGAGAAC AGGGACCTCT AAGGCATGCA GCGTCCCCAT CTCCACAGAG GCAGGGTGGC 
TGGAGTACAG ACGGTGGAGG GCGACTGCTG GTCTTCGCCC CAGGGAGGGA GGACGAGGGA 
GGGCCAGCTC AGAGCAGGGG TGCCGGCAGG GGCGGGCCCC AGCAGCAGGG ATACATGCCC 
CTTGAGGGTC CCCAGCAGTC TGGCAGCCTT CCAGAGGAGG GCTCCAAGCC CTCAAACAGC 
TGTGTCCTCA GGGAGACCAT GGTGCAGGCT TCTCCCAGCT CAAAGGCCCA GCAGACACCC 
TCAGCTCAGG ACTATGGACG AGGCAACATC ATATCCCCAT CCAGGATGCT GGACAAGAGC 
CCCTCACCGG CCTCTGGGCA CTTTGCCCAC CCATCCTTTG CTGCCAGCCT GAAAATGGGT 
CCCCCCAAGA GCAAGGCTGA AAAAATCAAG AGAAGTCCCA TGGACAAGGT GCTGAGGTTT 
GCAAGGCAGC CGCTGCTTCT ACTGGACAGG CCTGAGGGAG CCCATGCAGC CCCCCAGCCA 
TCCCTGGAGT GGGACCCTGC CCACTGGCCC ACAGGGAGGG GCGGGCTCCA GCGGAGGCCA 
GCCCTGGCCT GGGAGGCACC CGGGCGCTCC TGTTCTGAGT CCACCCTCTA CCCCATGCCT 
GTCCTCGTCC CCTTGGCAGT GGCCCCGCAG GAGAGCCACC GGACCTCAGC CCAAGCCCTG 
TTCCCCTTTG AGGCGTCACT GCTCACCTCA GTGGCCAGGA GGAAGCATCG CCGCTGGCAG 
TCCACCGTGG AGATCTCGGC CCGGGCCCGC CTGGCCAGCT GTCCTGAGTC TAACCTGGGG 
CCCCCCAGGC CCGTGGCCAG GAGAGCAGGT GGCCCACTGG CCCGGGGCCG TCCCTCACTG 
GTCCGCCAGG ACGCCTACAC CAGGAGCGAC TCAGAGCCCT CCAAGCACTC GGCCGAGTGT 
GACCCGCGGT TCCCGTCAGT CATCCCGGAG ACCAGCGAGG GAGAGTCCAG TGACCACACC 
ACCAACCGAT TCGGAGACCG TGAGTCCAGC AGCAGCGACG AGGAGGGCGG CGCCCAGAGC 
AGGGACTGTG ACCTGGCACT GGGCTATGTG GCGGCCGGGC ATGCGGAGCT GGCCTGGACC 
CAGGAGGCCC CGGTCAGCTC GGGGCCACTC CTGTCCCCCG TGCCCAAGCT GTGCCGTATT 
AAGGCCTCCA AGGCCCTGAA GAAGAAGATC CGCAGGTTCC AGCCGACGGC CCTGAAGGTC 
ATGACCATGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533809.1
CDS	274..2208
Translation	MSDGGSCSLSTSCASVCSDHISPSLGSLLPVAQAHKARPSMGDWRPRSVDETTVPAWRPQATEEGARPPGSVEDAGQPWGTFWPRPVSTGDLDRALPADTGLQKASADAELLGLLCQGVDIPLHVPDPKYRQDLVSQGGREVYPYPSPLHAVALQSPLFVLTKETPQRGGPSFPRESPRGPAGLNTIQTGPVLEAGPARARAYIDRLLHLWGRETPAKGSEGEQGPLRHAASPSPQRQGGWSTDGGGRLLVFAPGREDEGGPAQSRGAGRGGPQQQGYMPLEGPQQSGSLPEEGSKPSNSCVLRETMVQASPSSKAQQTPSAQDYGRGNIISPSRMLDKSPSPASGHFAHPSFAASLKMGPPKSKAEKIKRSPMDKVLRFARQPLLLLDRPEGAHAAPQPSLEWDPAHWPTGRGGLQRRPALAWEAPGRSCSESTLYPMPVLVPLAVAPQESHRTSAQALFPFEASLLTSVARRKHRRWQSTVEISARARLASCPESNLGPPRPVARRAGGPLARGRPSLVRQDAYTRSDSEPSKHSAECDPRFPSVIPETSEGESSDHTTNRFGDRESSSSDEEGGAQSRDCDLALGYVAAGHAELAWTQEAPVSSGPLLSPVPKLCRIKASKALKKKIRRFQPTALKVMTMV

Target ORF information:

RefSeq Version	XM_011535507.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535507.2

ORF Insert Sequence:

ATGAGCGACG GTGGATCCTG CTCCCTGTCC ACGTCCTGTG CCTCCGTCTG CAGTGACCAC 
ATCTCTCCCT CGCTGGGCAG TTTGTTGCCT GTGGCCCAGG CCCACAAGGC CAGGCCCAGC 
ATGGGGGACT GGAGGCCCCG GTCGGTTGAT GAGACTACTG TGCCAGCGTG GAGACCCCAG 
GCTACCGAGG AGGGCGCCAG GCCCCCAGGG AGCGTGGAGG ATGCAGGCCA GCCGTGGGGC 
ACATTCTGGC CCAGGCCTGT GTCTACAGGT GATCTTGACA GAGCCCTGCC GGCGGACACG 
GGGCTCCAGA AAGCCAGCGC GGACGCCGAG CTCCTCGGGC TCCTCTGCCA GGGGGTGGAT 
ATCCCGCTGC ACGTGCCGGA CCCCAAGTAT CGGCAGGACC TGGTGTCCCA GGGCGGCAGG 
GAGGTGTACC CGTACCCCAG CCCCCTGCAC GCCGTGGCTC TACAGAGCCC CCTGTTTGTC 
CTGACTAAGG AAACCCCACA GAGAGGTGGC CCCTCGTTCC CTAGGGAGAG CCCCAGGGGC 
CCCGCAGGTC TGAACACCAT CCAGACTGGG CCGGTCCTCG AGGCTGGCCC GGCCAGGGCC 
AGAGCTTATA TCGACAGGTT GCTGCATCTG TGGGGCCGGG AGACCCCAGC AAAGGGTAGC 
GAGGGAGAAC AGGGACCTCT AAGGCATGCA GCGTCCCCAT CTCCACAGAG GCAGGGTGGC 
TGGAGTACAG ACGGTGGAGG GCGACTGCTG GTCTTCGCCC CAGGGAGGGA GGACGAGGGA 
GGGCCAGCTC AGAGCAGGGG TGCCGGCAGG GGCGGGCCCC AGCAGCAGGG ATACATGCCC 
CTTGAGGGTC CCCAGCAGTC TGGCAGCCTT CCAGAGGAGG GCTCCAAGCC CTCAAACAGC 
TGTGTCCTCA GGGAGACCAT GGTGCAGGCT TCTCCCAGCT CAAAGGCCCA GCAGACACCC 
TCAGCTCAGG ACTATGGACG AGGCAACATC ATATCCCCAT CCAGGATGCT GGACAAGAGC 
CCCTCACCGG CCTCTGGGCA CTTTGCCCAC CCATCCTTTG CTGCCAGCCT GAAAATGGGT 
CCCCCCAAGA GCAAGGCTGA AAAAATCAAG AGAAGTCCCA TGGACAAGGT GCTGAGGTTT 
GCAAGGCAGC CGCTGCTTCT ACTGGACAGG CCTGAGGGAG CCCATGCAGC CCCCCAGCCA 
TCCCTGGAGT GGGACCCTGC CCACTGGCCC ACAGGGAGGG GCGGGCTCCA GCGGAGGCCA 
GCCCTGGCCT GGGAGGCACC CGGGCGCTCC TGTTCTGAGT CCACCCTCTA CCCCATGCCT 
GTCCTCGTCC CCTTGGCAGT GGCCCCGCAG GAGAGCCACC GGACCTCAGC CCAAGCCCTG 
TTCCCCTTTG AGGCGTCACT GCTCACCTCA GTGGCCAGGA GGAAGCATCG CCGCTGGCAG 
TCCACCGTGG AGATCTCGGC CCGGGCCCGC CTGGCCAGCT GTCCTGAGTC TAACCTGGGG 
CCCCCCAGGC CCGTGGCCAG GAGAGCAGGT GGCCCACTGG CCCGGGGCCG TCCCTCACTG 
GTCCGCCAGG ACGCCTACAC CAGGAGCGAC TCAGAGCCCT CCAAGCACTC GGCCGAGTGT 
GACCCGCGGT TCCCGTCAGT CATCCCGGAG ACCAGCGAGG GAGAGTCCAG TGACCACACC 
ACCAACCGAT TCGGAGACCG TGAGTCCAGC AGCAGCGACG AGGAGGGCGG CGCCCAGAGC 
AGGGACTGTG ACCTGGCACT GGGCTATGTG GCGGCCGGGC ATGCGGAGCT GGCCTGGACC 
CAGGAGGCCC CGGTCAGCTC GGGGCCACTC CTGTCCCCCG TGCCCAAGCT GTGCCGTATT 
AAGGCCTCCA AGGCCCTGAA GAAGAAGATC CGCAGGTTCC AGCCGACGGC CCTGAAGGTC 
ATGACCATGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DACT2 ( NM_001286351.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273280.1 Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 3, mRNA.

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CloneID	OHu12028
Clone ID Related Accession (Same CDS sequence)	NM_001286351.2 , NM_001286351.1
Accession Version	NM_001286351.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Homo sapiens(human)
Product	dapper homolog 2 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL606970.12, BC092498.1, BC111790.1 and AF318336.1. On May 31, 2019 this sequence version replaced NM_001286351.1. Transcript Variant: This variant (3) contains alternate 3' exon structure and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (c) has a distinct C-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC092498.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGACGC CGGGCGGACC CCCGGGGTCC GCGGGCTGGG ACCGCCGTAG GTTGGGCGCG 
AGGTTGCGCG CGGCGTTCGC GGGGCTGCAG GAGCTGCAGG GGCTGCGAGC CACGCAGCAG 
GAGCGGGTAC GGGGCGCCCT GGCCCTGCAG CCCCCGCCCG CGCCCGCCGC GCCCTGCGGC 
CCCCACGGCC TCCACGGCCC CGAGCAGCAG CTGGAGGCGG CGCTGGCCGC GCTGCAGGAG 
CAGCTGTCCC GGCTGAGACA ACAGGACATC GGCCTGAAGA CCCACCTGGA CCAGCTGGAC 
CTGCAGATTA GCAAGCTGCA GCTGGATGTG GGCACAGCCT CAGGGGAGGC CCTGGACAGC 
GACAGCAGGC CCAGCTCAGG CTTTTACGAG ATGAGCGACG GTGGATCCTG CTCCCTGTCC 
ACGTCCTGTG CCTCCGTCTG CAGTGACCAC ATCTCTCCCT CGCTGGGCAG TTTGTTGCCT 
GTGGCCCAGG CCCACAAGGC CAGGCCCAGC ATGGGGGACT GGAGGCCCCG GTCGGTTGAT 
GAGACTACTG TGCCAGCGTG GAGACCCCAG GCTACCGAGG AGGGCGCCAG GCCCCCAGGG 
AGCGTGGAGG ATGCAGGCCA GCCGTGGGGC ACATTCTGGC CCAGGCCTGT GTCTACAGAG 
CTGTGCAATG CTCCAGGTGA ACTTGACATG CATGCTCCGC CTGCAGGCTG CACCTCCAGT 
TCACTGACAG GTGTAGGTTC TGGTTTGAGA GGAAAGTGTG GCCTCTGTGG CTGTCAGCTC 
CCTTTCTGCT CAGTGAACAC AAGTTCAAAG ACAAAATCAT CAGGAATTTC AAGACAGCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273280.1
CDS	145..987
Translation	MWTPGGPPGSAGWDRRRLGARLRAAFAGLQELQGLRATQQERVRGALALQPPPAPAAPCGPHGLHGPEQQLEAALAALQEQLSRLRQQDIGLKTHLDQLDLQISKLQLDVGTASGEALDSDSRPSSGFYEMSDGGSCSLSTSCASVCSDHISPSLGSLLPVAQAHKARPSMGDWRPRSVDETTVPAWRPQATEEGARPPGSVEDAGQPWGTFWPRPVSTELCNAPGELDMHAPPAGCTSSSLTGVGSGLRGKCGLCGCQLPFCSVNTSSKTKSSGISRQQ

Target ORF information:

RefSeq Version	NM_001286351.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286351.2

ORF Insert Sequence:

ATGTGGACGC CGGGCGGACC CCCGGGGTCC GCGGGCTGGG ACCGCCGTAG GTTGGGCGCG 
AGGTTGCGCG CGGCGTTCGC GGGGCTGCAG GAGCTGCAGG GGCTGCGAGC CACGCAGCAG 
GAGCGGGTAC GGGGCGCCCT GGCCCTGCAG CCCCCGCCCG CGCCCGCCGC GCCCTGCGGC 
CCCCACGGCC TCCACGGCCC CGAGCAGCAG CTGGAGGCGG CGCTGGCCGC GCTGCAGGAG 
CAGCTGTCCC GGCTGAGACA ACAGGACATC GGCCTGAAGA CCCACCTGGA CCAGCTGGAC 
CTGCAGATTA GCAAGCTGCA GCTGGATGTG GGCACAGCCT CAGGGGAGGC CCTGGACAGC 
GACAGCAGGC CCAGCTCAGG CTTTTACGAG ATGAGCGACG GTGGATCCTG CTCCCTGTCC 
ACGTCCTGTG CCTCCGTCTG CAGTGACCAC ATCTCTCCCT CGCTGGGCAG TTTGTTGCCT 
GTGGCCCAGG CCCACAAGGC CAGGCCCAGC ATGGGGGACT GGAGGCCCCG GTCGGTTGAT 
GAGACTACTG TGCCAGCGTG GAGACCCCAG GCTACCGAGG AGGGCGCCAG GCCCCCAGGG 
AGCGTGGAGG ATGCAGGCCA GCCGTGGGGC ACATTCTGGC CCAGGCCTGT GTCTACAGAG 
CTGTGCAATG CTCCAGGTGA ACTTGACATG CATGCTCCGC CTGCAGGCTG CACCTCCAGT 
TCACTGACAG GTGTAGGTTC TGGTTTGAGA GGAAAGTGTG GCCTCTGTGG CTGTCAGCTC 
CCTTTCTGCT CAGTGAACAC AAGTTCAAAG ACAAAATCAT CAGGAATTTC AAGACAGCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DACT2 ( NM_001286351.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273280.1 Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu12028
Clone ID Related Accession (Same CDS sequence)	NM_001286351.2 , NM_001286351.1
Accession Version	NM_001286351.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	dapper homolog 2 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC092498.1, BC111790.1, AF318336.1 and AL606970.12. On Nov 2, 2013 this sequence version replaced XM_005266837.1. Transcript Variant: This variant (3) contains alternate 3' exon structure and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (c) has a distinct C-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC092498.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGACGC CGGGCGGACC CCCGGGGTCC GCGGGCTGGG ACCGCCGTAG GTTGGGCGCG 
AGGTTGCGCG CGGCGTTCGC GGGGCTGCAG GAGCTGCAGG GGCTGCGAGC CACGCAGCAG 
GAGCGGGTAC GGGGCGCCCT GGCCCTGCAG CCCCCGCCCG CGCCCGCCGC GCCCTGCGGC 
CCCCACGGCC TCCACGGCCC CGAGCAGCAG CTGGAGGCGG CGCTGGCCGC GCTGCAGGAG 
CAGCTGTCCC GGCTGAGACA ACAGGACATC GGCCTGAAGA CCCACCTGGA CCAGCTGGAC 
CTGCAGATTA GCAAGCTGCA GCTGGATGTG GGCACAGCCT CAGGGGAGGC CCTGGACAGC 
GACAGCAGGC CCAGCTCAGG CTTTTACGAG ATGAGCGACG GTGGATCCTG CTCCCTGTCC 
ACGTCCTGTG CCTCCGTCTG CAGTGACCAC ATCTCTCCCT CGCTGGGCAG TTTGTTGCCT 
GTGGCCCAGG CCCACAAGGC CAGGCCCAGC ATGGGGGACT GGAGGCCCCG GTCGGTTGAT 
GAGACTACTG TGCCAGCGTG GAGACCCCAG GCTACCGAGG AGGGCGCCAG GCCCCCAGGG 
AGCGTGGAGG ATGCAGGCCA GCCGTGGGGC ACATTCTGGC CCAGGCCTGT GTCTACAGAG 
CTGTGCAATG CTCCAGGTGA ACTTGACATG CATGCTCCGC CTGCAGGCTG CACCTCCAGT 
TCACTGACAG GTGTAGGTTC TGGTTTGAGA GGAAAGTGTG GCCTCTGTGG CTGTCAGCTC 
CCTTTCTGCT CAGTGAACAC AAGTTCAAAG ACAAAATCAT CAGGAATTTC AAGACAGCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273280.1
CDS	122..964
Translation	MWTPGGPPGSAGWDRRRLGARLRAAFAGLQELQGLRATQQERVRGALALQPPPAPAAPCGPHGLHGPEQQLEAALAALQEQLSRLRQQDIGLKTHLDQLDLQISKLQLDVGTASGEALDSDSRPSSGFYEMSDGGSCSLSTSCASVCSDHISPSLGSLLPVAQAHKARPSMGDWRPRSVDETTVPAWRPQATEEGARPPGSVEDAGQPWGTFWPRPVSTELCNAPGELDMHAPPAGCTSSSLTGVGSGLRGKCGLCGCQLPFCSVNTSSKTKSSGISRQQ

Target ORF information:

RefSeq Version	NM_001286351.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286351.1

ORF Insert Sequence:

ATGTGGACGC CGGGCGGACC CCCGGGGTCC GCGGGCTGGG ACCGCCGTAG GTTGGGCGCG 
AGGTTGCGCG CGGCGTTCGC GGGGCTGCAG GAGCTGCAGG GGCTGCGAGC CACGCAGCAG 
GAGCGGGTAC GGGGCGCCCT GGCCCTGCAG CCCCCGCCCG CGCCCGCCGC GCCCTGCGGC 
CCCCACGGCC TCCACGGCCC CGAGCAGCAG CTGGAGGCGG CGCTGGCCGC GCTGCAGGAG 
CAGCTGTCCC GGCTGAGACA ACAGGACATC GGCCTGAAGA CCCACCTGGA CCAGCTGGAC 
CTGCAGATTA GCAAGCTGCA GCTGGATGTG GGCACAGCCT CAGGGGAGGC CCTGGACAGC 
GACAGCAGGC CCAGCTCAGG CTTTTACGAG ATGAGCGACG GTGGATCCTG CTCCCTGTCC 
ACGTCCTGTG CCTCCGTCTG CAGTGACCAC ATCTCTCCCT CGCTGGGCAG TTTGTTGCCT 
GTGGCCCAGG CCCACAAGGC CAGGCCCAGC ATGGGGGACT GGAGGCCCCG GTCGGTTGAT 
GAGACTACTG TGCCAGCGTG GAGACCCCAG GCTACCGAGG AGGGCGCCAG GCCCCCAGGG 
AGCGTGGAGG ATGCAGGCCA GCCGTGGGGC ACATTCTGGC CCAGGCCTGT GTCTACAGAG 
CTGTGCAATG CTCCAGGTGA ACTTGACATG CATGCTCCGC CTGCAGGCTG CACCTCCAGT 
TCACTGACAG GTGTAGGTTC TGGTTTGAGA GGAAAGTGTG GCCTCTGTGG CTGTCAGCTC 
CCTTTCTGCT CAGTGAACAC AAGTTCAAAG ACAAAATCAT CAGGAATTTC AAGACAGCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DACT2 Epigenetic Stimulator Exerts Dual Efficacy for Colorectal Cancer Prevention and Treatment.
Pharmacological research(2017 12)
Lu L,Wang Y,Ou R,Feng Q,Ji L,Zheng H,Guo Y,Qi X,Kong AN,Liu Z

Methylation of DACT2 promotes breast cancer development by activating Wnt signaling.
Scientific reports7(1)3325(2017 Jun)
Li J,Zhang M,He T,Li H,Cao T,Zheng L,Guo M

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DACT2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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電話

FAX

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