Dact2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dact2
Entrez Gene ID	240025
Full Name	dishevelled-binding antagonist of beta-catenin 2
Synonyms	2900084M21Rik,A630024E20,Dpr2,Frd2,dapper2
General protein information	Preferred Names dishevelled-binding antagonist of beta-catenin 2 Names dapper homolog 2 dapper antagonist of catenin 2 dapper homolog 2, antagonist of beta-catenin dapper, antagonist of beta-catenin, homolog 2 mDpr2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 A2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006524227.4	XP_006524290.1	dapper homolog 2 isoform X1
XM_006524227.3	XP_006524290.1	dapper homolog 2 isoform X1
NM_172826.3	NP_766414.3	dapper homolog 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DACT2	XP_002690443.2
Pan troglodytes (chimpanzee)	DACT2	XP_001145122.2
Homo sapiens (human)	DACT2	NP_999627.2
	DACT2	XP_001083736.1
Mus musculus (house mouse)	Dact2	NP_766414.3
Rattus norvegicus (Norway rat)	Dact2	NP_001100934.2
Canis lupus familiaris (dog)	DACT2	XP_855310.3
Gallus gallus (chicken)	DACT2	XP_419602.4

Gene Ontology
Bibliography

Related articles in PubMed

DACT2 Epigenetic Stimulator Exerts Dual Efficacy for Colorectal Cancer Prevention and Treatment.
Lu L, Wang Y, Ou R, Feng Q, Ji L, Zheng H, Guo Y, Qi X, Kong AN, Liu Z
Pharmacological research(2017 12)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Identification of the genes regulated by Wnt-4, a critical signal for commitment of the ovary.
Naillat F, Yan W, Karjalainen R, Liakhovitskaia A, Samoylenko A, Xu Q, Sun Z, Shen B, Medvinsky A, Quaggin S, Vainio SJ
Experimental cell research332(2)163-78(2015 Mar)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Haploinsufficiency of SAMD9L, an endosome fusion facilitator, causes myeloid malignancies in mice mimicking human diseases with monosomy 7.
Nagamachi A, Matsui H, Asou H, Ozaki Y, Aki D, Kanai A, Takubo K, Suda T, Nakamura T, Wolff L, Honda H, Inaba T
Cancer cell24(3)305-17(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we have identified a novel role for Dact2 as an inhibitor of the canonical Wnt pathway in embryonic tooth development through its regulation of cell proliferation and differentiation.
Title: Dact2 represses PITX2 transcriptional activation and cell proliferation through Wnt/beta-catenin signaling during odontogenesis.

conserved role for Dact2 protein in kinase-regulated biochemistry involving Vangl and Dvl.
Title: All Dact (Dapper/Frodo) scaffold proteins dimerize and exhibit conserved interactions with Vangl, Dvl, and serine/threonine kinases.

Dact2 plays a role in regulating morphogenesis by renal collecting duct cells, probably by protecting cells from overly strong TGF-beta pathway activation.
Title: Dact2 is expressed in the developing ureteric bud/collecting duct system of the kidney and controls morphogenetic behavior of collecting duct cells.

Dact2 may play important signal-modulating roles in the adjacent epithelial cells including the enamel knot signaling centers and pre-ameloblasts.
Title: Dact1-3 mRNAs exhibit distinct expression domains during tooth development.

Dpr2 plays an inhibitory role in the re-epithelialization of adult skin wounds by attenuating TGFbeta signaling.
Title: Accelerated re-epithelialization in Dpr2-deficient mice is associated with enhanced response to TGFbeta signaling.

The following Dact2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dact2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18316	NM_172826.3 Latest version!	Mus musculus dishevelled-binding antagonist of beta-catenin 2 (Dact2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$447.30 $639.00
OMu53553	XM_006524227.4 Latest version!	Mus musculus dishevelled-binding antagonist of beta-catenin 2 (Dact2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu53553	XM_006524227.3	Mus musculus dishevelled-binding antagonist of beta-catenin 2 (Dact2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dact2 ( XM_006524227.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524290.1 Mus musculus dishevelled-binding antagonist of beta-catenin 2 (Dact2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53553
Clone ID Related Accession (Same CDS sequence)	XM_006524227.4 , XM_006524227.3
Accession Version	XM_006524227.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	dapper homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524227.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGAG CTGCACACCT CAGGAACTTT CTAGAAGCTT CCTTAAAGTC ACGGCTAAGG 
AGACAGGATG CTGGCCTGAA GACACACTTG GACCAGCTGG ATCAGCAGAT AAGTGAACTA 
CAGCTGGATG TGAGCAGGTC TTCTTGCGAG GCCTTGGATA GTGACAGCAG ACCCAGCTCA 
GGTTTCTATG AGCTGAGCGA TGCTGGTTCC TGTTCTCTGT CCACCTCCTG CGCATCTGTT 
TGCAGCGACC GTCTATCCCC CTCCCTGGGT AGCTGGCTGC CTGTGTTCCA GCCCTCCAAG 
TCCAGGTCTG GCATCGGGGA CTGGCGACCA CGCTCTGCTG ATGAGACCAC TGTCCCTGCA 
TGGAGCCCAC AGCTCACAGA AGATAGCAGG CTCCTGCATG GTGCAGAGGG CACAGGCCGG 
CTGACGGGCA TGTTCCGGCC CAGACCAGTA TCTACAGGTG ATCTCGAAAG AGTCCTTCCA 
GCTGACGTGG GGCTCCAGAG AGCTGGTACT GATGCTGCAC ATCTCCTGGG CCAGGGGATA 
GAGATCCCAG CCCACGCCCT GGACCCCACG TACCAGCGTG ACCTGGTGGC CAGGGGAGGC 
CAGGAGGTGT ACCCATATCC CAGCCCCCTC CATGCGGTGG CCCTGCAGAG TCCCCTCTTC 
GCTCTGCCCA AAGAAGCCCC GTGTTTTGAC ATCTGCTCAC CTCCCCAGGA GCCTCCTCTG 
GTCCCTGTTG ATGAGAACAG GACTCAACCT GAGCCGATCC GTGAGCTGGG CTCAGCCGAA 
GCCTACATCC ACAGGCTGTT GCATCTGCGG GGCCAAGAGC TCCCCCTGAG AGATGTGGGG 
CAGGAGCAGG GAGGTGACAC AGCTGCTTTT CCACCGAAGC CCTGTGGCCA GAGGTCGGAG 
AGCACATGTC AGCTGGAGAA GCAGGCCTGT GGAGCTGACA GGGGAGGACT GAAACTAGGT 
AGGGGTGCTG CCAAGGACAG CCTCAAGCAA CATGGGCCTG TGTCCCTTGT GGGTGCTGAG 
CCCCTCAGCA GCCCCCTAAA GGAAGAAACC ATTCCTTGGA ATCCCTGTGT CCATGGAGAC 
AATACTGTTG GTTCCTCACC CTGCTCCCAG GCCCAACAGC CTCTTAATGA CTGTGGCCAA 
GGACCAGTCC TGTCACCATC CAGGGTGTTG GGCACTGAGA GCCCACCTCT GGCCCCGGAG 
CCCTTTGCCT ATACATCCTG CACCACTGGT GAAACCTCTC CCGTGAAGCT GAGGATGGGC 
TTTTCCCAAA ACAAGGCCGT GAAGGTCAGA AGAAGAGTTA GTGAGAAAGT CCCGAGGCTA 
GGGAAGCAGC TCCCTCCACA ACCAGAGAGG CAGCGGGTCA CAGAGCGGGA CCCCTCCAGG 
CCCCATCAGG GAGGTCTCAG CAGGAGGCCC ACACTGGCCC GGGAGCCTCC TGGACGCTCC 
TGCTCTGAGT CCACCCTCTA CCCCGTGCCC TTCCTTGTCC CCGTAGTGGT GGCCCAGAGG 
GAAAGTTACC CAACGTCACC CCAAGCGTTC TTCCCAATGG AGGCAGCTCT CCTCAGCTCA 
GCAGCCAGGC GGAAGCAGCG CAGGTGGCAG TCCACCATGG AGATCTCAGC CAAAGCCGGT 
TCAGTCAGCC AACCTGGGCC CAGCATGGGG CTCCCTAGGT CCCCAGCCAA GAGAGGAAGT 
GGTCCCAGGG CCCAGAGTAG GCCCACACTT GCCCGTCAGG ATGCCTGTGC GAGGTGTGAG 
TCGGACCCCT CGGAGCACTC TGCAGACTGC ACCTCACTCT ACCACTCCAC CATTGCCGAG 
ACCAGCGAGG ACGAGGAGGC GAGTGACCAC ACTGCCAACC GCTTTGGGGA CGAGTCCAGC 
AGCAACGATT CAGAAGGGTG TTTCCGGGGC AGCCGCCGTC GCCTGGCAAT AGGCAGTGCA 
GAGGCTGGGC AAGGGGGGTG GGCCTGGCCT CGGGTGCCCC CCCAGCAGCC CTCACGGGCC 
CCAGGAAACA CCAGGCCACC CTTGCCCCCT GTGCCCAAAC TGTGCCGCAT CAAGGCCTCC 
AAGGCCCTGA AGAAGAAGAT CCGAAGGTTT CAGCCAGCAG CCCTGAAGGT CATGACCATG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524290.1
CDS	115..2220
Translation	MVRAAHLRNFLEASLKSRLRRQDAGLKTHLDQLDQQISELQLDVSRSSCEALDSDSRPSSGFYELSDAGSCSLSTSCASVCSDRLSPSLGSWLPVFQPSKSRSGIGDWRPRSADETTVPAWSPQLTEDSRLLHGAEGTGRLTGMFRPRPVSTGDLERVLPADVGLQRAGTDAAHLLGQGIEIPAHALDPTYQRDLVARGGQEVYPYPSPLHAVALQSPLFALPKEAPCFDICSPPQEPPLVPVDENRTQPEPIRELGSAEAYIHRLLHLRGQELPLRDVGQEQGGDTAAFPPKPCGQRSESTCQLEKQACGADRGGLKLGRGAAKDSLKQHGPVSLVGAEPLSSPLKEETIPWNPCVHGDNTVGSSPCSQAQQPLNDCGQGPVLSPSRVLGTESPPLAPEPFAYTSCTTGETSPVKLRMGFSQNKAVKVRRRVSEKVPRLGKQLPPQPERQRVTERDPSRPHQGGLSRRPTLAREPPGRSCSESTLYPVPFLVPVVVAQRESYPTSPQAFFPMEAALLSSAARRKQRRWQSTMEISAKAGSVSQPGPSMGLPRSPAKRGSGPRAQSRPTLARQDACARCESDPSEHSADCTSLYHSTIAETSEDEEASDHTANRFGDESSSNDSEGCFRGSRRRLAIGSAEAGQGGWAWPRVPPQQPSRAPGNTRPPLPPVPKLCRIKASKALKKKIRRFQPAALKVMTMV

Target ORF information:

RefSeq Version	XM_006524227.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus dishevelled-binding antagonist of beta-catenin 2 (Dact2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524227.3

ORF Insert Sequence:

ATGGTGAGAG CTGCACACCT CAGGAACTTT CTAGAAGCTT CCTTAAAGTC ACGGCTAAGG 
AGACAGGATG CTGGCCTGAA GACACACTTG GACCAGCTGG ATCAGCAGAT AAGTGAACTA 
CAGCTGGATG TGAGCAGGTC TTCTTGCGAG GCCTTGGATA GTGACAGCAG ACCCAGCTCA 
GGTTTCTATG AGCTGAGCGA TGCTGGTTCC TGTTCTCTGT CCACCTCCTG CGCATCTGTT 
TGCAGCGACC GTCTATCCCC CTCCCTGGGT AGCTGGCTGC CTGTGTTCCA GCCCTCCAAG 
TCCAGGTCTG GCATCGGGGA CTGGCGACCA CGCTCTGCTG ATGAGACCAC TGTCCCTGCA 
TGGAGCCCAC AGCTCACAGA AGATAGCAGG CTCCTGCATG GTGCAGAGGG CACAGGCCGG 
CTGACGGGCA TGTTCCGGCC CAGACCAGTA TCTACAGGTG ATCTCGAAAG AGTCCTTCCA 
GCTGACGTGG GGCTCCAGAG AGCTGGTACT GATGCTGCAC ATCTCCTGGG CCAGGGGATA 
GAGATCCCAG CCCACGCCCT GGACCCCACG TACCAGCGTG ACCTGGTGGC CAGGGGAGGC 
CAGGAGGTGT ACCCATATCC CAGCCCCCTC CATGCGGTGG CCCTGCAGAG TCCCCTCTTC 
GCTCTGCCCA AAGAAGCCCC GTGTTTTGAC ATCTGCTCAC CTCCCCAGGA GCCTCCTCTG 
GTCCCTGTTG ATGAGAACAG GACTCAACCT GAGCCGATCC GTGAGCTGGG CTCAGCCGAA 
GCCTACATCC ACAGGCTGTT GCATCTGCGG GGCCAAGAGC TCCCCCTGAG AGATGTGGGG 
CAGGAGCAGG GAGGTGACAC AGCTGCTTTT CCACCGAAGC CCTGTGGCCA GAGGTCGGAG 
AGCACATGTC AGCTGGAGAA GCAGGCCTGT GGAGCTGACA GGGGAGGACT GAAACTAGGT 
AGGGGTGCTG CCAAGGACAG CCTCAAGCAA CATGGGCCTG TGTCCCTTGT GGGTGCTGAG 
CCCCTCAGCA GCCCCCTAAA GGAAGAAACC ATTCCTTGGA ATCCCTGTGT CCATGGAGAC 
AATACTGTTG GTTCCTCACC CTGCTCCCAG GCCCAACAGC CTCTTAATGA CTGTGGCCAA 
GGACCAGTCC TGTCACCATC CAGGGTGTTG GGCACTGAGA GCCCACCTCT GGCCCCGGAG 
CCCTTTGCCT ATACATCCTG CACCACTGGT GAAACCTCTC CCGTGAAGCT GAGGATGGGC 
TTTTCCCAAA ACAAGGCCGT GAAGGTCAGA AGAAGAGTTA GTGAGAAAGT CCCGAGGCTA 
GGGAAGCAGC TCCCTCCACA ACCAGAGAGG CAGCGGGTCA CAGAGCGGGA CCCCTCCAGG 
CCCCATCAGG GAGGTCTCAG CAGGAGGCCC ACACTGGCCC GGGAGCCTCC TGGACGCTCC 
TGCTCTGAGT CCACCCTCTA CCCCGTGCCC TTCCTTGTCC CCGTAGTGGT GGCCCAGAGG 
GAAAGTTACC CAACGTCACC CCAAGCGTTC TTCCCAATGG AGGCAGCTCT CCTCAGCTCA 
GCAGCCAGGC GGAAGCAGCG CAGGTGGCAG TCCACCATGG AGATCTCAGC CAAAGCCGGT 
TCAGTCAGCC AACCTGGGCC CAGCATGGGG CTCCCTAGGT CCCCAGCCAA GAGAGGAAGT 
GGTCCCAGGG CCCAGAGTAG GCCCACACTT GCCCGTCAGG ATGCCTGTGC GAGGTGTGAG 
TCGGACCCCT CGGAGCACTC TGCAGACTGC ACCTCACTCT ACCACTCCAC CATTGCCGAG 
ACCAGCGAGG ACGAGGAGGC GAGTGACCAC ACTGCCAACC GCTTTGGGGA CGAGTCCAGC 
AGCAACGATT CAGAAGGGTG TTTCCGGGGC AGCCGCCGTC GCCTGGCAAT AGGCAGTGCA 
GAGGCTGGGC AAGGGGGGTG GGCCTGGCCT CGGGTGCCCC CCCAGCAGCC CTCACGGGCC 
CCAGGAAACA CCAGGCCACC CTTGCCCCCT GTGCCCAAAC TGTGCCGCAT CAAGGCCTCC 
AAGGCCCTGA AGAAGAAGAT CCGAAGGTTT CAGCCAGCAG CCCTGAAGGT CATGACCATG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dact2 ( NM_172826.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766414.3 Mus musculus dishevelled-binding antagonist of beta-catenin 2 (Dact2), mRNA.

Price & Availability↑

CloneID	OMu18316
Clone ID Related Accession (Same CDS sequence)	NM_172826.3
Accession Version	NM_172826.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	dapper homolog 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC151292.2 and BC058740.1. On Apr 3, 2009 this sequence version replaced NM_172826.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058740.1, AK141226.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCAC CGAGCGGCCA GGGGCCCGCG GGTTGGGACC GCCGCAGGGT GGGCGCGCGA 
CTACGAGCCG CACTGGCCGG GCTGCAGGAG CTTCAGGGGC TGCGAGCCAC GCAGCAAGCA 
CGCGTGCGGG GAGCTCTGGG CCTGCACCCT GCGCCAGGGC CCCGCGGCCA GGAGCTGCGT 
CTGGAGGCGG CGCTGACCGC GCTACGGGAG CAGCTGTCAC GGCTAAGGAG ACAGGATGCT 
GGCCTGAAGA CACACTTGGA CCAGCTGGAT CAGCAGATAA GTGAACTACA GCTGGATGTG 
AGCAGGTCTT CTTGCGAGGC CTTGGATAGT GACAGCAGAC CCAGCTCAGG TTTCTATGAG 
CTGAGCGATG CTGGTTCCTG TTCTCTGTCC ACCTCCTGCG CATCTGTTTG CAGCGACCGT 
CTATCCCCCT CCCTGGGTAG CTGGCTGCCT GTGTTCCAGC CCTCCAAGTC CAGGTCTGGC 
ATCGGGGACT GGCGACCACG CTCTGCTGAT GAGACCACTG TCCCTGCATG GAGCCCACAG 
CTCACAGAAG ATAGCAGGCT CCTGCATGGT GCAGAGGGCA CAGGCCGGCT GACGGGCATG 
TTCCGGCCCA GACCAGTATC TACAGGTGAT CTCGAAAGAG TCCTTCCAGC TGACGTGGGG 
CTCCAGAGAG CTGGTACTGA TGCTGCACAT CTCCTGGGCC AGGGGATAGA GATCCCAGCC 
CACGCCCTGG ACCCCACGTA CCAGCGTGAC CTGGTGGCCA GGGGAGGCCA GGAGGTGTAC 
CCATATCCCA GCCCCCTCCA TGCGGTGGCC CTGCAGAGTC CCCTCTTCGC TCTGCCCAAA 
GAAGCCCCGT GTTTTGACAT CTGCTCACCT CCCCAGGAGC CTCCTCTGGT CCCTGTTGAT 
GAGAACAGGA CTCAACCTGA GCCGATCCGT GAGCTGGGCT CAGCCGAAGC CTACATCCAC 
AGGCTGTTGC ATCTGCGGGG CCAAGAGCTC CCCCTGAGAG ATGTGGGGCA GGAGCAGGGA 
GGTGACACAG CTGCTTTTCC ACCGAAGCCC TGTGGCCAGA GGTCGGAGAG CACATGTCAG 
CTGGAGAAGC AGGCCTGTGG AGCTGACAGG GGAGGACTGA AACTAGGTAG GGGTGCTGCC 
AAGGACAGCC TCAAGCAACA TGGGCCTGTG TCCCTTGTGG GTGCTGAGCC CCTCAGCAGC 
CCCCTAAAGG AAGAAACCAT TCCTTGGAAT CCCTGTGTCC ATGGAGACAA TACTGTTGGT 
TCCTCACCCT GCTCCCAGGC CCAACAGCCT CTTAATGACT GTGGCCAAGG ACCAGTCCTG 
TCACCATCCA GGGTGTTGGG CACTGAGAGC CCACCTCTGG CCCCGGAGCC CTTTGCCTAT 
ACATCCTGCA CCACTGGTGA AACCTCTCCC GTGAAGCTGA GGATGGGCTT TTCCCAAAAC 
AAGGCCGTGA AGGTCAGAAG AAGAGTTAGT GAGAAAGTCC CGAGGCTAGG GAAGCAGCTC 
CCTCCACAAC CAGAGAGGCA GCGGGTCACA GAGCGGGACC CCTCCAGGCC CCATCAGGGA 
GGTCTCAGCA GGAGGCCCAC ACTGGCCCGG GAGCCTCCTG GACGCTCCTG CTCTGAGTCC 
ACCCTCTACC CCGTGCCCTT CCTTGTCCCC GTAGTGGTGG CCCAGAGGGA AAGTTACCCA 
ACGTCACCCC AAGCGTTCTT CCCAATGGAG GCAGCTCTCC TCAGCTCAGC AGCCAGGCGG 
AAGCAGCGCA GGTGGCAGTC CACCATGGAG ATCTCAGCCA AAGCCGGTTC AGTCAGCCAA 
CCTGGGCCCA GCATGGGGCT CCCTAGGTCC CCAGCCAAGA GAGGAAGTGG TCCCAGGGCC 
CAGAGTAGGC CCACACTTGC CCGTCAGGAT GCCTGTGCGA GGTGTGAGTC GGACCCCTCG 
GAGCACTCTG CAGACTGCAC CTCACTCTAC CACTCCACCA TTGCCGAGAC CAGCGAGGAC 
GAGGAGGCGA GTGACCACAC TGCCAACCGC TTTGGGGACG AGTCCAGCAG CAACGATTCA 
GAAGGGTGTT TCCGGGGCAG CCGCCGTCGC CTGGCAATAG GCAGTGCAGA GGCTGGGCAA 
GGGGGGTGGG CCTGGCCTCG GGTGCCCCCC CAGCAGCCCT CACGGGCCCC AGGAAACACC 
AGGCCACCCT TGCCCCCTGT GCCCAAACTG TGCCGCATCA AGGCCTCCAA GGCCCTGAAG 
AAGAAGATCC GAAGGTTTCA GCCAGCAGCC CTGAAGGTCA TGACCATGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766414.3
CDS	103..2376
Translation	MWAPSGQGPAGWDRRRVGARLRAALAGLQELQGLRATQQARVRGALGLHPAPGPRGQELRLEAALTALREQLSRLRRQDAGLKTHLDQLDQQISELQLDVSRSSCEALDSDSRPSSGFYELSDAGSCSLSTSCASVCSDRLSPSLGSWLPVFQPSKSRSGIGDWRPRSADETTVPAWSPQLTEDSRLLHGAEGTGRLTGMFRPRPVSTGDLERVLPADVGLQRAGTDAAHLLGQGIEIPAHALDPTYQRDLVARGGQEVYPYPSPLHAVALQSPLFALPKEAPCFDICSPPQEPPLVPVDENRTQPEPIRELGSAEAYIHRLLHLRGQELPLRDVGQEQGGDTAAFPPKPCGQRSESTCQLEKQACGADRGGLKLGRGAAKDSLKQHGPVSLVGAEPLSSPLKEETIPWNPCVHGDNTVGSSPCSQAQQPLNDCGQGPVLSPSRVLGTESPPLAPEPFAYTSCTTGETSPVKLRMGFSQNKAVKVRRRVSEKVPRLGKQLPPQPERQRVTERDPSRPHQGGLSRRPTLAREPPGRSCSESTLYPVPFLVPVVVAQRESYPTSPQAFFPMEAALLSSAARRKQRRWQSTMEISAKAGSVSQPGPSMGLPRSPAKRGSGPRAQSRPTLARQDACARCESDPSEHSADCTSLYHSTIAETSEDEEASDHTANRFGDESSSNDSEGCFRGSRRRLAIGSAEAGQGGWAWPRVPPQQPSRAPGNTRPPLPPVPKLCRIKASKALKKKIRRFQPAALKVMTMV

Target ORF information:

RefSeq Version	NM_172826.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus dishevelled-binding antagonist of beta-catenin 2 (Dact2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172826.3

ORF Insert Sequence:

ATGTGGGCAC CGAGCGGCCA GGGGCCCGCG GGTTGGGACC GCCGCAGGGT GGGCGCGCGA 
CTACGAGCCG CACTGGCCGG GCTGCAGGAG CTTCAGGGGC TGCGAGCCAC GCAGCAAGCA 
CGCGTGCGGG GAGCTCTGGG CCTGCACCCT GCGCCAGGGC CCCGCGGCCA GGAGCTGCGT 
CTGGAGGCGG CGCTGACCGC GCTACGGGAG CAGCTGTCAC GGCTAAGGAG ACAGGATGCT 
GGCCTGAAGA CACACTTGGA CCAGCTGGAT CAGCAGATAA GTGAACTACA GCTGGATGTG 
AGCAGGTCTT CTTGCGAGGC CTTGGATAGT GACAGCAGAC CCAGCTCAGG TTTCTATGAG 
CTGAGCGATG CTGGTTCCTG TTCTCTGTCC ACCTCCTGCG CATCTGTTTG CAGCGACCGT 
CTATCCCCCT CCCTGGGTAG CTGGCTGCCT GTGTTCCAGC CCTCCAAGTC CAGGTCTGGC 
ATCGGGGACT GGCGACCACG CTCTGCTGAT GAGACCACTG TCCCTGCATG GAGCCCACAG 
CTCACAGAAG ATAGCAGGCT CCTGCATGGT GCAGAGGGCA CAGGCCGGCT GACGGGCATG 
TTCCGGCCCA GACCAGTATC TACAGGTGAT CTCGAAAGAG TCCTTCCAGC TGACGTGGGG 
CTCCAGAGAG CTGGTACTGA TGCTGCACAT CTCCTGGGCC AGGGGATAGA GATCCCAGCC 
CACGCCCTGG ACCCCACGTA CCAGCGTGAC CTGGTGGCCA GGGGAGGCCA GGAGGTGTAC 
CCATATCCCA GCCCCCTCCA TGCGGTGGCC CTGCAGAGTC CCCTCTTCGC TCTGCCCAAA 
GAAGCCCCGT GTTTTGACAT CTGCTCACCT CCCCAGGAGC CTCCTCTGGT CCCTGTTGAT 
GAGAACAGGA CTCAACCTGA GCCGATCCGT GAGCTGGGCT CAGCCGAAGC CTACATCCAC 
AGGCTGTTGC ATCTGCGGGG CCAAGAGCTC CCCCTGAGAG ATGTGGGGCA GGAGCAGGGA 
GGTGACACAG CTGCTTTTCC ACCGAAGCCC TGTGGCCAGA GGTCGGAGAG CACATGTCAG 
CTGGAGAAGC AGGCCTGTGG AGCTGACAGG GGAGGACTGA AACTAGGTAG GGGTGCTGCC 
AAGGACAGCC TCAAGCAACA TGGGCCTGTG TCCCTTGTGG GTGCTGAGCC CCTCAGCAGC 
CCCCTAAAGG AAGAAACCAT TCCTTGGAAT CCCTGTGTCC ATGGAGACAA TACTGTTGGT 
TCCTCACCCT GCTCCCAGGC CCAACAGCCT CTTAATGACT GTGGCCAAGG ACCAGTCCTG 
TCACCATCCA GGGTGTTGGG CACTGAGAGC CCACCTCTGG CCCCGGAGCC CTTTGCCTAT 
ACATCCTGCA CCACTGGTGA AACCTCTCCC GTGAAGCTGA GGATGGGCTT TTCCCAAAAC 
AAGGCCGTGA AGGTCAGAAG AAGAGTTAGT GAGAAAGTCC CGAGGCTAGG GAAGCAGCTC 
CCTCCACAAC CAGAGAGGCA GCGGGTCACA GAGCGGGACC CCTCCAGGCC CCATCAGGGA 
GGTCTCAGCA GGAGGCCCAC ACTGGCCCGG GAGCCTCCTG GACGCTCCTG CTCTGAGTCC 
ACCCTCTACC CCGTGCCCTT CCTTGTCCCC GTAGTGGTGG CCCAGAGGGA AAGTTACCCA 
ACGTCACCCC AAGCGTTCTT CCCAATGGAG GCAGCTCTCC TCAGCTCAGC AGCCAGGCGG 
AAGCAGCGCA GGTGGCAGTC CACCATGGAG ATCTCAGCCA AAGCCGGTTC AGTCAGCCAA 
CCTGGGCCCA GCATGGGGCT CCCTAGGTCC CCAGCCAAGA GAGGAAGTGG TCCCAGGGCC 
CAGAGTAGGC CCACACTTGC CCGTCAGGAT GCCTGTGCGA GGTGTGAGTC GGACCCCTCG 
GAGCACTCTG CAGACTGCAC CTCACTCTAC CACTCCACCA TTGCCGAGAC CAGCGAGGAC 
GAGGAGGCGA GTGACCACAC TGCCAACCGC TTTGGGGACG AGTCCAGCAG CAACGATTCA 
GAAGGGTGTT TCCGGGGCAG CCGCCGTCGC CTGGCAATAG GCAGTGCAGA GGCTGGGCAA 
GGGGGGTGGG CCTGGCCTCG GGTGCCCCCC CAGCAGCCCT CACGGGCCCC AGGAAACACC 
AGGCCACCCT TGCCCCCTGT GCCCAAACTG TGCCGCATCA AGGCCTCCAA GGCCCTGAAG 
AAGAAGATCC GAAGGTTTCA GCCAGCAGCC CTGAAGGTCA TGACCATGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dact2 ( XM_006524227.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524290.1 Mus musculus dishevelled-binding antagonist of beta-catenin 2 (Dact2), transcript variant X1, mRNA.

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CloneID	OMu53553
Clone ID Related Accession (Same CDS sequence)	XM_006524227.4 , XM_006524227.3
Accession Version	XM_006524227.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	dapper homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524227.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGAG CTGCACACCT CAGGAACTTT CTAGAAGCTT CCTTAAAGTC ACGGCTAAGG 
AGACAGGATG CTGGCCTGAA GACACACTTG GACCAGCTGG ATCAGCAGAT AAGTGAACTA 
CAGCTGGATG TGAGCAGGTC TTCTTGCGAG GCCTTGGATA GTGACAGCAG ACCCAGCTCA 
GGTTTCTATG AGCTGAGCGA TGCTGGTTCC TGTTCTCTGT CCACCTCCTG CGCATCTGTT 
TGCAGCGACC GTCTATCCCC CTCCCTGGGT AGCTGGCTGC CTGTGTTCCA GCCCTCCAAG 
TCCAGGTCTG GCATCGGGGA CTGGCGACCA CGCTCTGCTG ATGAGACCAC TGTCCCTGCA 
TGGAGCCCAC AGCTCACAGA AGATAGCAGG CTCCTGCATG GTGCAGAGGG CACAGGCCGG 
CTGACGGGCA TGTTCCGGCC CAGACCAGTA TCTACAGGTG ATCTCGAAAG AGTCCTTCCA 
GCTGACGTGG GGCTCCAGAG AGCTGGTACT GATGCTGCAC ATCTCCTGGG CCAGGGGATA 
GAGATCCCAG CCCACGCCCT GGACCCCACG TACCAGCGTG ACCTGGTGGC CAGGGGAGGC 
CAGGAGGTGT ACCCATATCC CAGCCCCCTC CATGCGGTGG CCCTGCAGAG TCCCCTCTTC 
GCTCTGCCCA AAGAAGCCCC GTGTTTTGAC ATCTGCTCAC CTCCCCAGGA GCCTCCTCTG 
GTCCCTGTTG ATGAGAACAG GACTCAACCT GAGCCGATCC GTGAGCTGGG CTCAGCCGAA 
GCCTACATCC ACAGGCTGTT GCATCTGCGG GGCCAAGAGC TCCCCCTGAG AGATGTGGGG 
CAGGAGCAGG GAGGTGACAC AGCTGCTTTT CCACCGAAGC CCTGTGGCCA GAGGTCGGAG 
AGCACATGTC AGCTGGAGAA GCAGGCCTGT GGAGCTGACA GGGGAGGACT GAAACTAGGT 
AGGGGTGCTG CCAAGGACAG CCTCAAGCAA CATGGGCCTG TGTCCCTTGT GGGTGCTGAG 
CCCCTCAGCA GCCCCCTAAA GGAAGAAACC ATTCCTTGGA ATCCCTGTGT CCATGGAGAC 
AATACTGTTG GTTCCTCACC CTGCTCCCAG GCCCAACAGC CTCTTAATGA CTGTGGCCAA 
GGACCAGTCC TGTCACCATC CAGGGTGTTG GGCACTGAGA GCCCACCTCT GGCCCCGGAG 
CCCTTTGCCT ATACATCCTG CACCACTGGT GAAACCTCTC CCGTGAAGCT GAGGATGGGC 
TTTTCCCAAA ACAAGGCCGT GAAGGTCAGA AGAAGAGTTA GTGAGAAAGT CCCGAGGCTA 
GGGAAGCAGC TCCCTCCACA ACCAGAGAGG CAGCGGGTCA CAGAGCGGGA CCCCTCCAGG 
CCCCATCAGG GAGGTCTCAG CAGGAGGCCC ACACTGGCCC GGGAGCCTCC TGGACGCTCC 
TGCTCTGAGT CCACCCTCTA CCCCGTGCCC TTCCTTGTCC CCGTAGTGGT GGCCCAGAGG 
GAAAGTTACC CAACGTCACC CCAAGCGTTC TTCCCAATGG AGGCAGCTCT CCTCAGCTCA 
GCAGCCAGGC GGAAGCAGCG CAGGTGGCAG TCCACCATGG AGATCTCAGC CAAAGCCGGT 
TCAGTCAGCC AACCTGGGCC CAGCATGGGG CTCCCTAGGT CCCCAGCCAA GAGAGGAAGT 
GGTCCCAGGG CCCAGAGTAG GCCCACACTT GCCCGTCAGG ATGCCTGTGC GAGGTGTGAG 
TCGGACCCCT CGGAGCACTC TGCAGACTGC ACCTCACTCT ACCACTCCAC CATTGCCGAG 
ACCAGCGAGG ACGAGGAGGC GAGTGACCAC ACTGCCAACC GCTTTGGGGA CGAGTCCAGC 
AGCAACGATT CAGAAGGGTG TTTCCGGGGC AGCCGCCGTC GCCTGGCAAT AGGCAGTGCA 
GAGGCTGGGC AAGGGGGGTG GGCCTGGCCT CGGGTGCCCC CCCAGCAGCC CTCACGGGCC 
CCAGGAAACA CCAGGCCACC CTTGCCCCCT GTGCCCAAAC TGTGCCGCAT CAAGGCCTCC 
AAGGCCCTGA AGAAGAAGAT CCGAAGGTTT CAGCCAGCAG CCCTGAAGGT CATGACCATG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524290.1
CDS	105..2210
Translation	MVRAAHLRNFLEASLKSRLRRQDAGLKTHLDQLDQQISELQLDVSRSSCEALDSDSRPSSGFYELSDAGSCSLSTSCASVCSDRLSPSLGSWLPVFQPSKSRSGIGDWRPRSADETTVPAWSPQLTEDSRLLHGAEGTGRLTGMFRPRPVSTGDLERVLPADVGLQRAGTDAAHLLGQGIEIPAHALDPTYQRDLVARGGQEVYPYPSPLHAVALQSPLFALPKEAPCFDICSPPQEPPLVPVDENRTQPEPIRELGSAEAYIHRLLHLRGQELPLRDVGQEQGGDTAAFPPKPCGQRSESTCQLEKQACGADRGGLKLGRGAAKDSLKQHGPVSLVGAEPLSSPLKEETIPWNPCVHGDNTVGSSPCSQAQQPLNDCGQGPVLSPSRVLGTESPPLAPEPFAYTSCTTGETSPVKLRMGFSQNKAVKVRRRVSEKVPRLGKQLPPQPERQRVTERDPSRPHQGGLSRRPTLAREPPGRSCSESTLYPVPFLVPVVVAQRESYPTSPQAFFPMEAALLSSAARRKQRRWQSTMEISAKAGSVSQPGPSMGLPRSPAKRGSGPRAQSRPTLARQDACARCESDPSEHSADCTSLYHSTIAETSEDEEASDHTANRFGDESSSNDSEGCFRGSRRRLAIGSAEAGQGGWAWPRVPPQQPSRAPGNTRPPLPPVPKLCRIKASKALKKKIRRFQPAALKVMTMV

Target ORF information:

RefSeq Version	XM_006524227.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus dishevelled-binding antagonist of beta-catenin 2 (Dact2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524227.4

ORF Insert Sequence:

ATGGTGAGAG CTGCACACCT CAGGAACTTT CTAGAAGCTT CCTTAAAGTC ACGGCTAAGG 
AGACAGGATG CTGGCCTGAA GACACACTTG GACCAGCTGG ATCAGCAGAT AAGTGAACTA 
CAGCTGGATG TGAGCAGGTC TTCTTGCGAG GCCTTGGATA GTGACAGCAG ACCCAGCTCA 
GGTTTCTATG AGCTGAGCGA TGCTGGTTCC TGTTCTCTGT CCACCTCCTG CGCATCTGTT 
TGCAGCGACC GTCTATCCCC CTCCCTGGGT AGCTGGCTGC CTGTGTTCCA GCCCTCCAAG 
TCCAGGTCTG GCATCGGGGA CTGGCGACCA CGCTCTGCTG ATGAGACCAC TGTCCCTGCA 
TGGAGCCCAC AGCTCACAGA AGATAGCAGG CTCCTGCATG GTGCAGAGGG CACAGGCCGG 
CTGACGGGCA TGTTCCGGCC CAGACCAGTA TCTACAGGTG ATCTCGAAAG AGTCCTTCCA 
GCTGACGTGG GGCTCCAGAG AGCTGGTACT GATGCTGCAC ATCTCCTGGG CCAGGGGATA 
GAGATCCCAG CCCACGCCCT GGACCCCACG TACCAGCGTG ACCTGGTGGC CAGGGGAGGC 
CAGGAGGTGT ACCCATATCC CAGCCCCCTC CATGCGGTGG CCCTGCAGAG TCCCCTCTTC 
GCTCTGCCCA AAGAAGCCCC GTGTTTTGAC ATCTGCTCAC CTCCCCAGGA GCCTCCTCTG 
GTCCCTGTTG ATGAGAACAG GACTCAACCT GAGCCGATCC GTGAGCTGGG CTCAGCCGAA 
GCCTACATCC ACAGGCTGTT GCATCTGCGG GGCCAAGAGC TCCCCCTGAG AGATGTGGGG 
CAGGAGCAGG GAGGTGACAC AGCTGCTTTT CCACCGAAGC CCTGTGGCCA GAGGTCGGAG 
AGCACATGTC AGCTGGAGAA GCAGGCCTGT GGAGCTGACA GGGGAGGACT GAAACTAGGT 
AGGGGTGCTG CCAAGGACAG CCTCAAGCAA CATGGGCCTG TGTCCCTTGT GGGTGCTGAG 
CCCCTCAGCA GCCCCCTAAA GGAAGAAACC ATTCCTTGGA ATCCCTGTGT CCATGGAGAC 
AATACTGTTG GTTCCTCACC CTGCTCCCAG GCCCAACAGC CTCTTAATGA CTGTGGCCAA 
GGACCAGTCC TGTCACCATC CAGGGTGTTG GGCACTGAGA GCCCACCTCT GGCCCCGGAG 
CCCTTTGCCT ATACATCCTG CACCACTGGT GAAACCTCTC CCGTGAAGCT GAGGATGGGC 
TTTTCCCAAA ACAAGGCCGT GAAGGTCAGA AGAAGAGTTA GTGAGAAAGT CCCGAGGCTA 
GGGAAGCAGC TCCCTCCACA ACCAGAGAGG CAGCGGGTCA CAGAGCGGGA CCCCTCCAGG 
CCCCATCAGG GAGGTCTCAG CAGGAGGCCC ACACTGGCCC GGGAGCCTCC TGGACGCTCC 
TGCTCTGAGT CCACCCTCTA CCCCGTGCCC TTCCTTGTCC CCGTAGTGGT GGCCCAGAGG 
GAAAGTTACC CAACGTCACC CCAAGCGTTC TTCCCAATGG AGGCAGCTCT CCTCAGCTCA 
GCAGCCAGGC GGAAGCAGCG CAGGTGGCAG TCCACCATGG AGATCTCAGC CAAAGCCGGT 
TCAGTCAGCC AACCTGGGCC CAGCATGGGG CTCCCTAGGT CCCCAGCCAA GAGAGGAAGT 
GGTCCCAGGG CCCAGAGTAG GCCCACACTT GCCCGTCAGG ATGCCTGTGC GAGGTGTGAG 
TCGGACCCCT CGGAGCACTC TGCAGACTGC ACCTCACTCT ACCACTCCAC CATTGCCGAG 
ACCAGCGAGG ACGAGGAGGC GAGTGACCAC ACTGCCAACC GCTTTGGGGA CGAGTCCAGC 
AGCAACGATT CAGAAGGGTG TTTCCGGGGC AGCCGCCGTC GCCTGGCAAT AGGCAGTGCA 
GAGGCTGGGC AAGGGGGGTG GGCCTGGCCT CGGGTGCCCC CCCAGCAGCC CTCACGGGCC 
CCAGGAAACA CCAGGCCACC CTTGCCCCCT GTGCCCAAAC TGTGCCGCAT CAAGGCCTCC 
AAGGCCCTGA AGAAGAAGAT CCGAAGGTTT CAGCCAGCAG CCCTGAAGGT CATGACCATG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DACT2 Epigenetic Stimulator Exerts Dual Efficacy for Colorectal Cancer Prevention and Treatment.
Pharmacological research(2017 12)
Lu L,Wang Y,Ou R,Feng Q,Ji L,Zheng H,Guo Y,Qi X,Kong AN,Liu Z

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Identification of the genes regulated by Wnt-4, a critical signal for commitment of the ovary.
Experimental cell research332(2)163-78(2015 Mar)
Naillat F,Yan W,Karjalainen R,Liakhovitskaia A,Samoylenko A,Xu Q,Sun Z,Shen B,Medvinsky A,Quaggin S,Vainio SJ

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Haploinsufficiency of SAMD9L, an endosome fusion facilitator, causes myeloid malignancies in mice mimicking human diseases with monosomy 7.
Cancer cell24(3)305-17(2013 Sep)
Nagamachi A,Matsui H,Asou H,Ozaki Y,Aki D,Kanai A,Takubo K,Suda T,Nakamura T,Wolff L,Honda H,Inaba T

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Dact2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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