FGD5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FGD5
Entrez Gene ID	152273
Full Name	FYVE, RhoGEF and PH domain containing 5
Synonyms	ZFYVE23
General protein information	Preferred Names FYVE, RhoGEF and PH domain containing 5 Names FYVE, RhoGEF and PH domain-containing protein 5 zinc finger FYVE domain-containing protein 23
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p25.1
Disorder MIM:	614788

mRNA and Protein(s)

mRNA	Protein	Name
NM_152536.3	NP_689749.3	FYVE, RhoGEF and PH domain-containing protein 5 isoform 1
XM_011533422.1	XP_011531724.1	FYVE, RhoGEF and PH domain-containing protein 5 isoform X1
NM_152536.4	NP_689749.3	FYVE, RhoGEF and PH domain-containing protein 5 isoform 1
NM_001320276.1	NP_001307205.1	FYVE, RhoGEF and PH domain-containing protein 5 isoform 2
XM_011533423.1	XP_011531725.1	FYVE, RhoGEF and PH domain-containing protein 5 isoform X2

Pathways from BioSystems

Homologies

	FGD5	XP_002808033.1
Canis lupus familiaris (dog)	FGD5	XP_541754.3
Bos taurus (cattle)	FGD5	XP_005223201.1
Rattus norvegicus (Norway rat)	Fgd5	XP_006236988.1
Gallus gallus (chicken)	FGD5	XP_414463.4
Xenopus tropicalis (tropical clawed frog)	fgd5	XP_002938283.2
Pan troglodytes (chimpanzee)	FGD5	XP_516303.3
Mus musculus (house mouse)	Fgd5	NP_766319.2
Homo sapiens (human)	FGD5	NP_689749.3

Gene Ontology
Bibliography

Related articles in PubMed

FGD5 Regulates VEGF Receptor-2 Coupling to PI3 Kinase and Receptor Recycling.
Farhan MA, Azad AK, Touret N, Murray AG
Arteriosclerosis, thrombosis, and vascular biology37(12)2301-2310(2017 Dec)

FGD5 sustains vascular endothelial growth factor A (VEGFA) signaling through inhibition of proteasome-mediated VEGF receptor 2 degradation.
Heldin J, O'Callaghan P, Hern?ndez Vera R, Fuchs PF, Gerwins P, Kreuger J
Cellular signalling40125-132(2017 Dec)

FGD5 amplification in breast cancer patients is associated with tumour proliferation and a poorer prognosis.
Valla M, Engstr?m MJ, Ytterhus B, Hansen ?K, Akslen LA, Vatten LJ, Opdahl S, Bofin AM
Breast cancer research and treatment162(2)243-253(2017 04)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FGD5 regulates VEGFR2 retention in recycling endosomes and coupling to PI3 (phosphoinositide-3) kinase/mTORC2-dependent cytoskeletal remodeling in endothelial cells.
Title: FGD5 Regulates VEGF Receptor-2 Coupling to PI3 Kinase and Receptor Recycling.

FGD5 is a new prognostic marker in breast cancer, and increased copy number is associated with higher tumour proliferation and poorer long-term prognosis.
Title: FGD5 amplification in breast cancer patients is associated with tumour proliferation and a poorer prognosis.

We provide the first report that pro-angiogenic genes PECAM1, PTGS1, FGD5, and MCAM may play a vital role in pathological dermal angiogenesis disorders of psoriasis.
Title: Expression of pro-angiogenic genes in mesenchymal stem cells derived from dermis of patients with psoriasis.

FGD5 regulates endothelial adhesion, survival, and angiogenesis by modulating phosphatidylinositol 3-kinase signaling.
Title: Facio-genital dysplasia-5 regulates matrix adhesion and survival of human endothelial cells.

FGD5 inhibits neovascularization, indicated by in vitro tube-formation, aortic-ring, and coated-bead assays and by in vivo coated-bead plug assays and studies in the murine retina model.
Title: Endothelial cell-specific FGD5 involvement in vascular pruning defines neovessel fate in mice.

The following FGD5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FGD5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06830	NM_152536.4 Latest version!	Homo sapiens FYVE, RhoGEF and PH domain containing 5 (FGD5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu76722	NM_001320276.1 Latest version!	Homo sapiens FYVE, RhoGEF and PH domain containing 5 (FGD5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu76720	XM_011533422.1 Latest version!	Homo sapiens FYVE, RhoGEF and PH domain containing 5 (FGD5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu76721	XM_011533423.1 Latest version!	Homo sapiens FYVE, RhoGEF and PH domain containing 5 (FGD5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu06830	NM_152536.3	Homo sapiens FYVE, RhoGEF and PH domain containing 5 (FGD5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FGD5 ( NM_152536.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_689749.3 Homo sapiens FYVE, RhoGEF and PH domain containing 5 (FGD5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06830
Clone ID Related Accession (Same CDS sequence)	NM_152536.3 , NM_152536.4
Accession Version	NM_152536.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-16
Organism	Homo sapiens(human)
Product	FYVE, RhoGEF and PH domain-containing protein 5 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC087591.2 and AC090937.2. On Jul 10, 2008 this sequence version replaced NM_152536.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK131078.1, BC132816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCAGGG GTCCGAAGCC CCCCATTGCC CCCAAGCCCA GGCTGACTGC CCCAAACGAG 
TGGAGAGCCA GTGTGTACCT GAATGACAGC TTGAACAAAT GCAGCAACGG GCGGCTGCCC 
TGTGTAGACA GGGGGCTTGA TGAGGGGCCC CGGTCCATCC CAAAGTGCTC TGAGTCGGAG 
ACCGACGAGG ATTACATCGT GGTCCCCAGG GTTCCGCTGA GGGAGGATGA ACCCAAGGAC 
GAGGGCAGTG TGGGGAACAA AGCCCTGGTG TCTCCCGAGT CCTCTGCGGA AGAGGAAGAG 
GAGCGTGAAG AGGGAGGCGA GGCATGTGGC CTGGAGGGTA CAGGAGCTGG TGAGGATTCA 
GTGGCCCCTG CTGCTCCGGG TGCAGGAGCG CTGAGCAGGG AGGGTGAGGA AGGCACAGAC 
CTTGCTCTTG AGGATGAAGG GGAGGGCTGC GCTGATGAGC CAGGGACACT GGAGCAGGTG 
TCCAGAAGTG AGGAGGAAGA GAAGCTAGTG CAGCCACACA GGGAGTGCAG CCTGGAGGAC 
AGTGGGCCTT GGGCTGGAGA GGGGGTCTTC CAGAGCGACC TCCTCCTGCC TCACATCCAT 
GGAGAGGACC AGGAGCCCCC CGACACCCCC GGGGAGGCAG AGGAGGATGA TGAGGAAGGC 
TGTGCCAGCA CAGACCCAGC AGGGGCAGAT GAGGGTTCGG GTCCTGACAG GCCCACGGAG 
GACATGGGAC AGGATGCTGA GGACACCAGT GAGGAGCCCC CTGAGAAGGA GGAGCTGGCC 
GGGGTCCAGG AGGCAGAGAC AGCCACAGAC TGCCCTGAAG TTCTTGAGGA GGGATGTGAA 
GAGGCCACGG GTGTCACAGG TGGGGAACAG GTTGACCTCA GTGAACCACC TGACCACGAG 
AAGAAAACCA ACCAAGAAGT GGCAGCCGCC ACCCTGGAGG ACCATGCACA GGATGAGTCC 
GCCGAGGAGA GCTGCCAGAT TGTCCCTTTT GAGAATGACT GCATGGAGGA CTTCGTGACT 
TCCCTCACAG GAAGCCCCTA TGAGTTCTTC CCAACTGAGA GCACCTCTTT TTGCAGCGAG 
AGCTGTTCTC CTCTTTCTGA ATCAGCGAAA GGTTTAGAAT CAGAGCAGGC ACCAAAGCTG 
GGGCTGCGTG CGGAGGAGAA CCCCATGGTG GGGGCTTTGT GTGGCCAGTG TGGCTCCCTA 
CAGGGTGGAG CGGCCGAGGG TCCCGCAGCC CCTGATGTGG TGGTCGTGCT GGAGGAGGAG 
GCCTTGGATG ATGCACTGGC CAACCCCTAT GTGATGGGAG TGGGCCTGCC CGGTCAGGCG 
GCCCCTGGAG AAGGAGGGCA GGCTGCATCG GACGCCCTGG GTGGTTATGG CTCGAAAGAA 
GAATTGAACT GTGAGGCAGA GGGTGGCCTG GTTCCCGCGG ACAGGAAGAA CACCAGCACG 
AGGGTCCGGC CCCACTCTGG GAAGGTGGCC GGCTATGTCC CAGAAACCGT CCCTGAAGAA 
ACCGGACCTG AGGCGGGCTC GTCAGCCCCT GGCATTGGAG GTGCCGCAGA GGAGGTGGGA 
AAGACGCTTT TGTCATTGGA GGGGAAGCCC TTGGAAGCCA GCAGGGCCTT GCCAGCAAAG 
CCCAGGGCCT TTACTTTATA CCCTCGGTCG TTCTCCGTGG AAGGCCGAGA GATTCCAGTG 
TCCGTGTACC AGGAGCCTGA GGGGTCAGGG TTGGATGACC ACAGGATAAA GAGGAAAGAG 
GACAATCTCT CTCTGTCGTG TGTAATTGGC TCCTCTGGGA GTTTCTCCCA GAGAAACCAC 
CTTCCGTCCA GCGGCACCTC CACGCCTTCT TCCATGGTCG ACATCCCACC TCCTTTCGAC 
CTGGCCTGCA TCACCAAGAA GCCCATCACA AAGAGCTCTC CCTCACTCCT GATCGAGAGC 
GACTCCCCGG ACAAGTACAA GAAGAAGAAG TCATCCTTTA AGCGCTTCCT GGCACTGACG 
TTTAAGAAGA AGACGGAGAA CAAATTGCAT GTGGATGTGA ACGTGTCTTC CTCTAGGTCC 
TCTTCAGAGT CCAGCTACCA CGGGCCTTCC AGGATTCTGG AAGTTGACCG GAGAAGCCTC 
AGCAACTCCC CTCAGCTTAA GTCTCGGACT GGGAAGCTCC GGGCTTCTGA ATCCCCCTCC 
TCCCTCATCT TTTATAGAGA TGGCAAGAGG AAAGGTGTCC CCTTCAGCAG GACGGTGTCC 
AGAGTGGAGT CCTTTGAAGA CCGCTCCCGG CCGCCCTTCC TGCCCTTGCC ACTGACCAAG 
CCACGGTCCA TCTCCTTCCC CAGCGCTGAC ACTTCAGACT ATGAGAACAT TCCAGCCATG 
AACTCGGACT ATGAGAATAT CCAGATTCCA CCCCGGAGAC CTGCCAGGGC TGGCGCGTTC 
ACGAAGCTGT TTGAAGATCA GAGCAGAGCC CTGTCCACAG CAAACGAAAA TGATGGCTAC 
GTGGACATGA GCAGCTTCAA CGCCTTTGAG AGCAAACAGC AGAGTGCAGA CCAGGACGCA 
GAAAGCGCCT ACACAGAGCC CTACAAAGTC TGTCCCATCT CGTCGGCAGC CCCCAAAGAG 
GACCTTACGT CGGATGAAGA GCAGAGAAGC TCGGAGGAGG AGGACAGTGC TTCAAGAGAC 
CCCAGTGTCA CCCACAAGGT GGAAGGACAG TCCAGAGCCC TTGTCATCGC ACAGGAACTG 
CTATCTTCAG AGAAAGCATA CGTGGAGATG CTCCAGCACT TAAATCTGGA TTTCCATGGA 
GCTGTCATGA GGGCCTTGGA TGACATGGAC CATGAAGGCA GAGACACATT GGCCCGGGAG 
GAGCTGAGGC AGGGCCTGAG TGAACTCCCA GCCATCCACG ACCTTCATCA AGGCATCCTG 
GAGGAGCTGG AGGAAAGGCT GTCAAATTGG GAGAGCCAGC AGAAGGTAGC TGACGTCTTC 
CTGGCCCGGG AGCAGGGGTT TGATCACCAC GCCACTCACA TCCTGCAGTT CGACAGGTAC 
CTAGGTCTGC TCAGTGAGAA TTGCCTCCAC TCTCCCCGGC TGGCAGCTGC TGTCCGTGAA 
TTTGAGCAGA GTGTACAAGG AGGCAGCCAG ACTGCGAAGC ATCGGCTGCT GCGGGTGGTT 
CAACGCCTCT TCCAGTACCA AGTGCTCCTC ACAGACTATT TAAACAACCT TTGTCCGGAC 
TCCGCCGAGT ACGACAACAC ACAGGGTGCA CTGAGCCTCA TCTCCAAAGT CACAGACCGT 
GCCAACGACA GCATGGAGCA AGGGGAAAAC CTGCAGAAGC TGGTCCACAT TGAGCACAGC 
GTCCGGGGCC AAGGGGATCT CCTCCAGCCA GGAAGGGAGT TTCTGAAGGA AGGGACGCTG 
ATGAAAGTAA CAGGGAAAAA CAGACGGCCC CGGCACCTAT TTCTGATGAA CGATGTGCTC 
CTGTACACCT ATCCCCAGAA GGATGGGAAG TACCGGCTGA AGAACACATT GGCTGTGGCC 
AACATGAAGG TCAGCCGCCC TGTGATGGAG AAAGTGCCCT ACGCTCTAAA GATTGAGACT 
TCCGAGTCCT GCCTGATGCT GTCTGCGAGC TCCTGTGCAG AGAGGGACGA GTGGTATGGC 
TGTCTGAGCA GAGCCCTCCC TGAGGACTAC AAGGCCCAGG CGCTGGCTGC ATTCCACCAT 
AGCGTGGAGA TACGAGAGAG GCTGGGGGTT AGCCTTGGGG AGAGGCCCCC CACCCTGGTG 
CCTGTCACAC ACGTCATGAT GTGCATGAAC TGCGGCTGCG ACTTCTCCCT CACCCTGCGG 
CGTCATCACT GTCACGCCTG TGGCAAGATC GTGTGCCGGA ACTGTTCGCG GAACAAGTAC 
CCGCTGAAGT ACCTGAAGGA CAGGATGGCC AAGGTCTGCG ACGGCTGCTT CGGGGAGCTG 
AAGAAGCGGG GCAGGGCTGT CCCGGGCCTG ATGAGAGAGC GGCCTGTGAG CATGAGCTTC 
CCGCTGTCTT CACCCCGCTT CTCGGGCAGT GCCTTTTCAT CCGTCTTCCA GAGCATTAAC 
CCCTCGACCT TCAAGAAGCA GAAGAAAGTC CCTTCAGCCC TGACAGAGGT GGCTGCCTCT 
GGAGAGGGCT CTGCCATCAG TGGCTATCTC AGCCGGTGTA AGAGGGGCAA GCGGCACTGG 
AAGAAGCTCT GGTTTGTCAT CAAAGGCAAA GTTCTCTACA CCTACATGGC CAGTGAGGAC 
AAAGTGGCCT TGGAGAGTAT GCCTCTGCTA GGCTTCACCA TTGCTCCAGA AAAGGAAGAG 
GGCAGCAGTG AAGTAGGACC TATTTTTCAC CTTTACCACA AGAAAACCCT ATTTTATAGC 
TTCAAAGCAG AAGATACCAA TTCAGCTCAG AGGTGGATCG AGGCCATGGA AGATGCGAGT 
GTGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689749.3
CDS	111..4499
Translation	MFRGPKPPIAPKPRLTAPNEWRASVYLNDSLNKCSNGRLPCVDRGLDEGPRSIPKCSESETDEDYIVVPRVPLREDEPKDEGSVGNKALVSPESSAEEEEEREEGGEACGLEGTGAGEDSVAPAAPGAGALSREGEEGTDLALEDEGEGCADEPGTLEQVSRSEEEEKLVQPHRECSLEDSGPWAGEGVFQSDLLLPHIHGEDQEPPDTPGEAEEDDEEGCASTDPAGADEGSGPDRPTEDMGQDAEDTSEEPPEKEELAGVQEAETATDCPEVLEEGCEEATGVTGGEQVDLSEPPDHEKKTNQEVAAATLEDHAQDESAEESCQIVPFENDCMEDFVTSLTGSPYEFFPTESTSFCSESCSPLSESAKGLESEQAPKLGLRAEENPMVGALCGQCGSLQGGAAEGPAAPDVVVVLEEEALDDALANPYVMGVGLPGQAAPGEGGQAASDALGGYGSKEELNCEAEGGLVPADRKNTSTRVRPHSGKVAGYVPETVPEETGPEAGSSAPGIGGAAEEVGKTLLSLEGKPLEASRALPAKPRAFTLYPRSFSVEGREIPVSVYQEPEGSGLDDHRIKRKEDNLSLSCVIGSSGSFSQRNHLPSSGTSTPSSMVDIPPPFDLACITKKPITKSSPSLLIESDSPDKYKKKKSSFKRFLALTFKKKTENKLHVDVNVSSSRSSSESSYHGPSRILEVDRRSLSNSPQLKSRTGKLRASESPSSLIFYRDGKRKGVPFSRTVSRVESFEDRSRPPFLPLPLTKPRSISFPSADTSDYENIPAMNSDYENIQIPPRRPARAGAFTKLFEDQSRALSTANENDGYVDMSSFNAFESKQQSADQDAESAYTEPYKVCPISSAAPKEDLTSDEEQRSSEEEDSASRDPSVTHKVEGQSRALVIAQELLSSEKAYVEMLQHLNLDFHGAVMRALDDMDHEGRDTLAREELRQGLSELPAIHDLHQGILEELEERLSNWESQQKVADVFLAREQGFDHHATHILQFDRYLGLLSENCLHSPRLAAAVREFEQSVQGGSQTAKHRLLRVVQRLFQYQVLLTDYLNNLCPDSAEYDNTQGALSLISKVTDRANDSMEQGENLQKLVHIEHSVRGQGDLLQPGREFLKEGTLMKVTGKNRRPRHLFLMNDVLLYTYPQKDGKYRLKNTLAVANMKVSRPVMEKVPYALKIETSESCLMLSASSCAERDEWYGCLSRALPEDYKAQALAAFHHSVEIRERLGVSLGERPPTLVPVTHVMMCMNCGCDFSLTLRRHHCHACGKIVCRNCSRNKYPLKYLKDRMAKVCDGCFGELKKRGRAVPGLMRERPVSMSFPLSSPRFSGSAFSSVFQSINPSTFKKQKKVPSALTEVAASGEGSAISGYLSRCKRGKRHWKKLWFVIKGKVLYTYMASEDKVALESMPLLGFTIAPEKEEGSSEVGPIFHLYHKKTLFYSFKAEDTNSAQRWIEAMEDASVL

Target ORF information:

RefSeq Version	NM_152536.3
Organism	Homo sapiens(human)
Definition	Homo sapiens FYVE, RhoGEF and PH domain containing 5 (FGD5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152536.3

ORF Insert Sequence:

ATGTTCAGGG GTCCGAAGCC CCCCATTGCC CCCAAGCCCA GGCTGACTGC CCCAAACGAG 
TGGAGAGCCA GTGTGTACCT GAATGACAGC TTGAACAAAT GCAGCAACGG GCGGCTGCCC 
TGTGTAGACA GGGGGCTTGA TGAGGGGCCC CGGTCCATCC CAAAGTGCTC TGAGTCGGAG 
ACCGACGAGG ATTACATCGT GGTCCCCAGG GTTCCGCTGA GGGAGGATGA ACCCAAGGAC 
GAGGGCAGTG TGGGGAACAA AGCCCTGGTG TCTCCCGAGT CCTCTGCGGA AGAGGAAGAG 
GAGCGTGAAG AGGGAGGCGA GGCATGTGGC CTGGAGGGTA CAGGAGCTGG TGAGGATTCA 
GTGGCCCCTG CTGCTCCGGG TGCAGGAGCG CTGAGCAGGG AGGGTGAGGA AGGCACAGAC 
CTTGCTCTTG AGGATGAAGG GGAGGGCTGC GCTGATGAGC CAGGGACACT GGAGCAGGTG 
TCCAGAAGTG AGGAGGAAGA GAAGCTAGTG CAGCCACACA GGGAGTGCAG CCTGGAGGAC 
AGTGGGCCTT GGGCTGGAGA GGGGGTCTTC CAGAGCGACC TCCTCCTGCC TCACATCCAT 
GGAGAGGACC AGGAGCCCCC CGACACCCCC GGGGAGGCAG AGGAGGATGA TGAGGAAGGC 
TGTGCCAGCA CAGACCCAGC AGGGGCAGAT GAGGGTTCGG GTCCTGACAG GCCCACGGAG 
GACATGGGAC AGGATGCTGA GGACACCAGT GAGGAGCCCC CTGAGAAGGA GGAGCTGGCC 
GGGGTCCAGG AGGCAGAGAC AGCCACAGAC TGCCCTGAAG TTCTTGAGGA GGGATGTGAA 
GAGGCCACGG GTGTCACAGG TGGGGAACAG GTTGACCTCA GTGAACCACC TGACCACGAG 
AAGAAAACCA ACCAAGAAGT GGCAGCCGCC ACCCTGGAGG ACCATGCACA GGATGAGTCC 
GCCGAGGAGA GCTGCCAGAT TGTCCCTTTT GAGAATGACT GCATGGAGGA CTTCGTGACT 
TCCCTCACAG GAAGCCCCTA TGAGTTCTTC CCAACTGAGA GCACCTCTTT TTGCAGCGAG 
AGCTGTTCTC CTCTTTCTGA ATCAGCGAAA GGTTTAGAAT CAGAGCAGGC ACCAAAGCTG 
GGGCTGCGTG CGGAGGAGAA CCCCATGGTG GGGGCTTTGT GTGGCCAGTG TGGCTCCCTA 
CAGGGTGGAG CGGCCGAGGG TCCCGCAGCC CCTGATGTGG TGGTCGTGCT GGAGGAGGAG 
GCCTTGGATG ATGCACTGGC CAACCCCTAT GTGATGGGAG TGGGCCTGCC CGGTCAGGCG 
GCCCCTGGAG AAGGAGGGCA GGCTGCATCG GACGCCCTGG GTGGTTATGG CTCGAAAGAA 
GAATTGAACT GTGAGGCAGA GGGTGGCCTG GTTCCCGCGG ACAGGAAGAA CACCAGCACG 
AGGGTCCGGC CCCACTCTGG GAAGGTGGCC GGCTATGTCC CAGAAACCGT CCCTGAAGAA 
ACCGGACCTG AGGCGGGCTC GTCAGCCCCT GGCATTGGAG GTGCCGCAGA GGAGGTGGGA 
AAGACGCTTT TGTCATTGGA GGGGAAGCCC TTGGAAGCCA GCAGGGCCTT GCCAGCAAAG 
CCCAGGGCCT TTACTTTATA CCCTCGGTCG TTCTCCGTGG AAGGCCGAGA GATTCCAGTG 
TCCGTGTACC AGGAGCCTGA GGGGTCAGGG TTGGATGACC ACAGGATAAA GAGGAAAGAG 
GACAATCTCT CTCTGTCGTG TGTAATTGGC TCCTCTGGGA GTTTCTCCCA GAGAAACCAC 
CTTCCGTCCA GCGGCACCTC CACGCCTTCT TCCATGGTCG ACATCCCACC TCCTTTCGAC 
CTGGCCTGCA TCACCAAGAA GCCCATCACA AAGAGCTCTC CCTCACTCCT GATCGAGAGC 
GACTCCCCGG ACAAGTACAA GAAGAAGAAG TCATCCTTTA AGCGCTTCCT GGCACTGACG 
TTTAAGAAGA AGACGGAGAA CAAATTGCAT GTGGATGTGA ACGTGTCTTC CTCTAGGTCC 
TCTTCAGAGT CCAGCTACCA CGGGCCTTCC AGGATTCTGG AAGTTGACCG GAGAAGCCTC 
AGCAACTCCC CTCAGCTTAA GTCTCGGACT GGGAAGCTCC GGGCTTCTGA ATCCCCCTCC 
TCCCTCATCT TTTATAGAGA TGGCAAGAGG AAAGGTGTCC CCTTCAGCAG GACGGTGTCC 
AGAGTGGAGT CCTTTGAAGA CCGCTCCCGG CCGCCCTTCC TGCCCTTGCC ACTGACCAAG 
CCACGGTCCA TCTCCTTCCC CAGCGCTGAC ACTTCAGACT ATGAGAACAT TCCAGCCATG 
AACTCGGACT ATGAGAATAT CCAGATTCCA CCCCGGAGAC CTGCCAGGGC TGGCGCGTTC 
ACGAAGCTGT TTGAAGATCA GAGCAGAGCC CTGTCCACAG CAAACGAAAA TGATGGCTAC 
GTGGACATGA GCAGCTTCAA CGCCTTTGAG AGCAAACAGC AGAGTGCAGA CCAGGACGCA 
GAAAGCGCCT ACACAGAGCC CTACAAAGTC TGTCCCATCT CGTCGGCAGC CCCCAAAGAG 
GACCTTACGT CGGATGAAGA GCAGAGAAGC TCGGAGGAGG AGGACAGTGC TTCAAGAGAC 
CCCAGTGTCA CCCACAAGGT GGAAGGACAG TCCAGAGCCC TTGTCATCGC ACAGGAACTG 
CTATCTTCAG AGAAAGCATA CGTGGAGATG CTCCAGCACT TAAATCTGGA TTTCCATGGA 
GCTGTCATGA GGGCCTTGGA TGACATGGAC CATGAAGGCA GAGACACATT GGCCCGGGAG 
GAGCTGAGGC AGGGCCTGAG TGAACTCCCA GCCATCCACG ACCTTCATCA AGGCATCCTG 
GAGGAGCTGG AGGAAAGGCT GTCAAATTGG GAGAGCCAGC AGAAGGTAGC TGACGTCTTC 
CTGGCCCGGG AGCAGGGGTT TGATCACCAC GCCACTCACA TCCTGCAGTT CGACAGGTAC 
CTAGGTCTGC TCAGTGAGAA TTGCCTCCAC TCTCCCCGGC TGGCAGCTGC TGTCCGTGAA 
TTTGAGCAGA GTGTACAAGG AGGCAGCCAG ACTGCGAAGC ATCGGCTGCT GCGGGTGGTT 
CAACGCCTCT TCCAGTACCA AGTGCTCCTC ACAGACTATT TAAACAACCT TTGTCCGGAC 
TCCGCCGAGT ACGACAACAC ACAGGGTGCA CTGAGCCTCA TCTCCAAAGT CACAGACCGT 
GCCAACGACA GCATGGAGCA AGGGGAAAAC CTGCAGAAGC TGGTCCACAT TGAGCACAGC 
GTCCGGGGCC AAGGGGATCT CCTCCAGCCA GGAAGGGAGT TTCTGAAGGA AGGGACGCTG 
ATGAAAGTAA CAGGGAAAAA CAGACGGCCC CGGCACCTAT TTCTGATGAA CGATGTGCTC 
CTGTACACCT ATCCCCAGAA GGATGGGAAG TACCGGCTGA AGAACACATT GGCTGTGGCC 
AACATGAAGG TCAGCCGCCC TGTGATGGAG AAAGTGCCCT ACGCTCTAAA GATTGAGACT 
TCCGAGTCCT GCCTGATGCT GTCTGCGAGC TCCTGTGCAG AGAGGGACGA GTGGTATGGC 
TGTCTGAGCA GAGCCCTCCC TGAGGACTAC AAGGCCCAGG CGCTGGCTGC ATTCCACCAT 
AGCGTGGAGA TACGAGAGAG GCTGGGGGTT AGCCTTGGGG AGAGGCCCCC CACCCTGGTG 
CCTGTCACAC ACGTCATGAT GTGCATGAAC TGCGGCTGCG ACTTCTCCCT CACCCTGCGG 
CGTCATCACT GTCACGCCTG TGGCAAGATC GTGTGCCGGA ACTGTTCGCG GAACAAGTAC 
CCGCTGAAGT ACCTGAAGGA CAGGATGGCC AAGGTCTGCG ACGGCTGCTT CGGGGAGCTG 
AAGAAGCGGG GCAGGGCTGT CCCGGGCCTG ATGAGAGAGC GGCCTGTGAG CATGAGCTTC 
CCGCTGTCTT CACCCCGCTT CTCGGGCAGT GCCTTTTCAT CCGTCTTCCA GAGCATTAAC 
CCCTCGACCT TCAAGAAGCA GAAGAAAGTC CCTTCAGCCC TGACAGAGGT GGCTGCCTCT 
GGAGAGGGCT CTGCCATCAG TGGCTATCTC AGCCGGTGTA AGAGGGGCAA GCGGCACTGG 
AAGAAGCTCT GGTTTGTCAT CAAAGGCAAA GTTCTCTACA CCTACATGGC CAGTGAGGAC 
AAAGTGGCCT TGGAGAGTAT GCCTCTGCTA GGCTTCACCA TTGCTCCAGA AAAGGAAGAG 
GGCAGCAGTG AAGTAGGACC TATTTTTCAC CTTTACCACA AGAAAACCCT ATTTTATAGC 
TTCAAAGCAG AAGATACCAA TTCAGCTCAG AGGTGGATCG AGGCCATGGA AGATGCGAGT 
GTGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FGD5 ( NM_152536.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_689749.3 Homo sapiens FYVE, RhoGEF and PH domain containing 5 (FGD5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06830
Clone ID Related Accession (Same CDS sequence)	NM_152536.3 , NM_152536.4
Accession Version	NM_152536.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	FYVE, RhoGEF and PH domain-containing protein 5 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC087591.2 and AC090937.2. On May 17, 2019 this sequence version replaced NM_152536.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC132816.1, AK131078.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000285046.10/ ENSP00000285046.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCAGGG GTCCGAAGCC CCCCATTGCC CCCAAGCCCA GGCTGACTGC CCCAAACGAG 
TGGAGAGCCA GTGTGTACCT GAATGACAGC TTGAACAAAT GCAGCAACGG GCGGCTGCCC 
TGTGTAGACA GGGGGCTTGA TGAGGGGCCC CGGTCCATCC CAAAGTGCTC TGAGTCGGAG 
ACCGACGAGG ATTACATCGT GGTCCCCAGG GTTCCGCTGA GGGAGGATGA ACCCAAGGAC 
GAGGGCAGTG TGGGGAACAA AGCCCTGGTG TCTCCCGAGT CCTCTGCGGA AGAGGAAGAG 
GAGCGTGAAG AGGGAGGCGA GGCATGTGGC CTGGAGGGTA CAGGAGCTGG TGAGGATTCA 
GTGGCCCCTG CTGCTCCGGG TGCAGGAGCG CTGAGCAGGG AGGGTGAGGA AGGCACAGAC 
CTTGCTCTTG AGGATGAAGG GGAGGGCTGC GCTGATGAGC CAGGGACACT GGAGCAGGTG 
TCCAGAAGTG AGGAGGAAGA GAAGCTAGTG CAGCCACACA GGGAGTGCAG CCTGGAGGAC 
AGTGGGCCTT GGGCTGGAGA GGGGGTCTTC CAGAGCGACC TCCTCCTGCC TCACATCCAT 
GGAGAGGACC AGGAGCCCCC CGACACCCCC GGGGAGGCAG AGGAGGATGA TGAGGAAGGC 
TGTGCCAGCA CAGACCCAGC AGGGGCAGAT GAGGGTTCGG GTCCTGACAG GCCCACGGAG 
GACATGGGAC AGGATGCTGA GGACACCAGT GAGGAGCCCC CTGAGAAGGA GGAGCTGGCC 
GGGGTCCAGG AGGCAGAGAC AGCCACAGAC TGCCCTGAAG TTCTTGAGGA GGGATGTGAA 
GAGGCCACGG GTGTCACAGG TGGGGAACAG GTTGACCTCA GTGAACCACC TGACCACGAG 
AAGAAAACCA ACCAAGAAGT GGCAGCCGCC ACCCTGGAGG ACCATGCACA GGATGAGTCC 
GCCGAGGAGA GCTGCCAGAT TGTCCCTTTT GAGAATGACT GCATGGAGGA CTTCGTGACT 
TCCCTCACAG GAAGCCCCTA TGAGTTCTTC CCAACTGAGA GCACCTCTTT TTGCAGCGAG 
AGCTGTTCTC CTCTTTCTGA ATCAGCGAAA GGTTTAGAAT CAGAGCAGGC ACCAAAGCTG 
GGGCTGCGTG CGGAGGAGAA CCCCATGGTG GGGGCTTTGT GTGGCCAGTG TGGCTCCCTA 
CAGGGTGGAG CGGCCGAGGG TCCCGCAGCC CCTGATGTGG TGGTCGTGCT GGAGGAGGAG 
GCCTTGGATG ATGCACTGGC CAACCCCTAT GTGATGGGAG TGGGCCTGCC CGGTCAGGCG 
GCCCCTGGAG AAGGAGGGCA GGCTGCATCG GACGCCCTGG GTGGTTATGG CTCGAAAGAA 
GAATTGAACT GTGAGGCAGA GGGTGGCCTG GTTCCCGCGG ACAGGAAGAA CACCAGCACG 
AGGGTCCGGC CCCACTCTGG GAAGGTGGCC GGCTATGTCC CAGAAACCGT CCCTGAAGAA 
ACCGGACCTG AGGCGGGCTC GTCAGCCCCT GGCATTGGAG GTGCCGCAGA GGAGGTGGGA 
AAGACGCTTT TGTCATTGGA GGGGAAGCCC TTGGAAGCCA GCAGGGCCTT GCCAGCAAAG 
CCCAGGGCCT TTACTTTATA CCCTCGGTCG TTCTCCGTGG AAGGCCGAGA GATTCCAGTG 
TCCGTGTACC AGGAGCCTGA GGGGTCAGGG TTGGATGACC ACAGGATAAA GAGGAAAGAG 
GACAATCTCT CTCTGTCGTG TGTAATTGGC TCCTCTGGGA GTTTCTCCCA GAGAAACCAC 
CTTCCGTCCA GCGGCACCTC CACGCCTTCT TCCATGGTCG ACATCCCACC TCCTTTCGAC 
CTGGCCTGCA TCACCAAGAA GCCCATCACA AAGAGCTCTC CCTCACTCCT GATCGAGAGC 
GACTCCCCGG ACAAGTACAA GAAGAAGAAG TCATCCTTTA AGCGCTTCCT GGCACTGACG 
TTTAAGAAGA AGACGGAGAA CAAATTGCAT GTGGATGTGA ACGTGTCTTC CTCTAGGTCC 
TCTTCAGAGT CCAGCTACCA CGGGCCTTCC AGGATTCTGG AAGTTGACCG GAGAAGCCTC 
AGCAACTCCC CTCAGCTTAA GTCTCGGACT GGGAAGCTCC GGGCTTCTGA ATCCCCCTCC 
TCCCTCATCT TTTATAGAGA TGGCAAGAGG AAAGGTGTCC CCTTCAGCAG GACGGTGTCC 
AGAGTGGAGT CCTTTGAAGA CCGCTCCCGG CCGCCCTTCC TGCCCTTGCC ACTGACCAAG 
CCACGGTCCA TCTCCTTCCC CAGCGCTGAC ACTTCAGACT ATGAGAACAT TCCAGCCATG 
AACTCGGACT ATGAGAATAT CCAGATTCCA CCCCGGAGAC CTGCCAGGGC TGGCGCGTTC 
ACGAAGCTGT TTGAAGATCA GAGCAGAGCC CTGTCCACAG CAAACGAAAA TGATGGCTAC 
GTGGACATGA GCAGCTTCAA CGCCTTTGAG AGCAAACAGC AGAGTGCAGA CCAGGACGCA 
GAAAGCGCCT ACACAGAGCC CTACAAAGTC TGTCCCATCT CGTCGGCAGC CCCCAAAGAG 
GACCTTACGT CGGATGAAGA GCAGAGAAGC TCGGAGGAGG AGGACAGTGC TTCAAGAGAC 
CCCAGTGTCA CCCACAAGGT GGAAGGACAG TCCAGAGCCC TTGTCATCGC ACAGGAACTG 
CTATCTTCAG AGAAAGCATA CGTGGAGATG CTCCAGCACT TAAATCTGGA TTTCCATGGA 
GCTGTCATGA GGGCCTTGGA TGACATGGAC CATGAAGGCA GAGACACATT GGCCCGGGAG 
GAGCTGAGGC AGGGCCTGAG TGAACTCCCA GCCATCCACG ACCTTCATCA AGGCATCCTG 
GAGGAGCTGG AGGAAAGGCT GTCAAATTGG GAGAGCCAGC AGAAGGTAGC TGACGTCTTC 
CTGGCCCGGG AGCAGGGGTT TGATCACCAC GCCACTCACA TCCTGCAGTT CGACAGGTAC 
CTAGGTCTGC TCAGTGAGAA TTGCCTCCAC TCTCCCCGGC TGGCAGCTGC TGTCCGTGAA 
TTTGAGCAGA GTGTACAAGG AGGCAGCCAG ACTGCGAAGC ATCGGCTGCT GCGGGTGGTT 
CAACGCCTCT TCCAGTACCA AGTGCTCCTC ACAGACTATT TAAACAACCT TTGTCCGGAC 
TCCGCCGAGT ACGACAACAC ACAGGGTGCA CTGAGCCTCA TCTCCAAAGT CACAGACCGT 
GCCAACGACA GCATGGAGCA AGGGGAAAAC CTGCAGAAGC TGGTCCACAT TGAGCACAGC 
GTCCGGGGCC AAGGGGATCT CCTCCAGCCA GGAAGGGAGT TTCTGAAGGA AGGGACGCTG 
ATGAAAGTAA CAGGGAAAAA CAGACGGCCC CGGCACCTAT TTCTGATGAA CGATGTGCTC 
CTGTACACCT ATCCCCAGAA GGATGGGAAG TACCGGCTGA AGAACACATT GGCTGTGGCC 
AACATGAAGG TCAGCCGCCC TGTGATGGAG AAAGTGCCCT ACGCTCTAAA GATTGAGACT 
TCCGAGTCCT GCCTGATGCT GTCTGCGAGC TCCTGTGCAG AGAGGGACGA GTGGTATGGC 
TGTCTGAGCA GAGCCCTCCC TGAGGACTAC AAGGCCCAGG CGCTGGCTGC ATTCCACCAT 
AGCGTGGAGA TACGAGAGAG GCTGGGGGTT AGCCTTGGGG AGAGGCCCCC CACCCTGGTG 
CCTGTCACAC ACGTCATGAT GTGCATGAAC TGCGGCTGCG ACTTCTCCCT CACCCTGCGG 
CGTCATCACT GTCACGCCTG TGGCAAGATC GTGTGCCGGA ACTGTTCGCG GAACAAGTAC 
CCGCTGAAGT ACCTGAAGGA CAGGATGGCC AAGGTCTGCG ACGGCTGCTT CGGGGAGCTG 
AAGAAGCGGG GCAGGGCTGT CCCGGGCCTG ATGAGAGAGC GGCCTGTGAG CATGAGCTTC 
CCGCTGTCTT CACCCCGCTT CTCGGGCAGT GCCTTTTCAT CCGTCTTCCA GAGCATTAAC 
CCCTCGACCT TCAAGAAGCA GAAGAAAGTC CCTTCAGCCC TGACAGAGGT GGCTGCCTCT 
GGAGAGGGCT CTGCCATCAG TGGCTATCTC AGCCGGTGTA AGAGGGGCAA GCGGCACTGG 
AAGAAGCTCT GGTTTGTCAT CAAAGGCAAA GTTCTCTACA CCTACATGGC CAGTGAGGAC 
AAAGTGGCCT TGGAGAGTAT GCCTCTGCTA GGCTTCACCA TTGCTCCAGA AAAGGAAGAG 
GGCAGCAGTG AAGTAGGACC TATTTTTCAC CTTTACCACA AGAAAACCCT ATTTTATAGC 
TTCAAAGCAG AAGATACCAA TTCAGCTCAG AGGTGGATCG AGGCCATGGA AGATGCGAGT 
GTGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689749.3
CDS	111..4499
Translation	MFRGPKPPIAPKPRLTAPNEWRASVYLNDSLNKCSNGRLPCVDRGLDEGPRSIPKCSESETDEDYIVVPRVPLREDEPKDEGSVGNKALVSPESSAEEEEEREEGGEACGLEGTGAGEDSVAPAAPGAGALSREGEEGTDLALEDEGEGCADEPGTLEQVSRSEEEEKLVQPHRECSLEDSGPWAGEGVFQSDLLLPHIHGEDQEPPDTPGEAEEDDEEGCASTDPAGADEGSGPDRPTEDMGQDAEDTSEEPPEKEELAGVQEAETATDCPEVLEEGCEEATGVTGGEQVDLSEPPDHEKKTNQEVAAATLEDHAQDESAEESCQIVPFENDCMEDFVTSLTGSPYEFFPTESTSFCSESCSPLSESAKGLESEQAPKLGLRAEENPMVGALCGQCGSLQGGAAEGPAAPDVVVVLEEEALDDALANPYVMGVGLPGQAAPGEGGQAASDALGGYGSKEELNCEAEGGLVPADRKNTSTRVRPHSGKVAGYVPETVPEETGPEAGSSAPGIGGAAEEVGKTLLSLEGKPLEASRALPAKPRAFTLYPRSFSVEGREIPVSVYQEPEGSGLDDHRIKRKEDNLSLSCVIGSSGSFSQRNHLPSSGTSTPSSMVDIPPPFDLACITKKPITKSSPSLLIESDSPDKYKKKKSSFKRFLALTFKKKTENKLHVDVNVSSSRSSSESSYHGPSRILEVDRRSLSNSPQLKSRTGKLRASESPSSLIFYRDGKRKGVPFSRTVSRVESFEDRSRPPFLPLPLTKPRSISFPSADTSDYENIPAMNSDYENIQIPPRRPARAGAFTKLFEDQSRALSTANENDGYVDMSSFNAFESKQQSADQDAESAYTEPYKVCPISSAAPKEDLTSDEEQRSSEEEDSASRDPSVTHKVEGQSRALVIAQELLSSEKAYVEMLQHLNLDFHGAVMRALDDMDHEGRDTLAREELRQGLSELPAIHDLHQGILEELEERLSNWESQQKVADVFLAREQGFDHHATHILQFDRYLGLLSENCLHSPRLAAAVREFEQSVQGGSQTAKHRLLRVVQRLFQYQVLLTDYLNNLCPDSAEYDNTQGALSLISKVTDRANDSMEQGENLQKLVHIEHSVRGQGDLLQPGREFLKEGTLMKVTGKNRRPRHLFLMNDVLLYTYPQKDGKYRLKNTLAVANMKVSRPVMEKVPYALKIETSESCLMLSASSCAERDEWYGCLSRALPEDYKAQALAAFHHSVEIRERLGVSLGERPPTLVPVTHVMMCMNCGCDFSLTLRRHHCHACGKIVCRNCSRNKYPLKYLKDRMAKVCDGCFGELKKRGRAVPGLMRERPVSMSFPLSSPRFSGSAFSSVFQSINPSTFKKQKKVPSALTEVAASGEGSAISGYLSRCKRGKRHWKKLWFVIKGKVLYTYMASEDKVALESMPLLGFTIAPEKEEGSSEVGPIFHLYHKKTLFYSFKAEDTNSAQRWIEAMEDASVL

Target ORF information:

RefSeq Version	NM_152536.4
Organism	Homo sapiens(human)
Definition	Homo sapiens FYVE, RhoGEF and PH domain containing 5 (FGD5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152536.4

ORF Insert Sequence:

ATGTTCAGGG GTCCGAAGCC CCCCATTGCC CCCAAGCCCA GGCTGACTGC CCCAAACGAG 
TGGAGAGCCA GTGTGTACCT GAATGACAGC TTGAACAAAT GCAGCAACGG GCGGCTGCCC 
TGTGTAGACA GGGGGCTTGA TGAGGGGCCC CGGTCCATCC CAAAGTGCTC TGAGTCGGAG 
ACCGACGAGG ATTACATCGT GGTCCCCAGG GTTCCGCTGA GGGAGGATGA ACCCAAGGAC 
GAGGGCAGTG TGGGGAACAA AGCCCTGGTG TCTCCCGAGT CCTCTGCGGA AGAGGAAGAG 
GAGCGTGAAG AGGGAGGCGA GGCATGTGGC CTGGAGGGTA CAGGAGCTGG TGAGGATTCA 
GTGGCCCCTG CTGCTCCGGG TGCAGGAGCG CTGAGCAGGG AGGGTGAGGA AGGCACAGAC 
CTTGCTCTTG AGGATGAAGG GGAGGGCTGC GCTGATGAGC CAGGGACACT GGAGCAGGTG 
TCCAGAAGTG AGGAGGAAGA GAAGCTAGTG CAGCCACACA GGGAGTGCAG CCTGGAGGAC 
AGTGGGCCTT GGGCTGGAGA GGGGGTCTTC CAGAGCGACC TCCTCCTGCC TCACATCCAT 
GGAGAGGACC AGGAGCCCCC CGACACCCCC GGGGAGGCAG AGGAGGATGA TGAGGAAGGC 
TGTGCCAGCA CAGACCCAGC AGGGGCAGAT GAGGGTTCGG GTCCTGACAG GCCCACGGAG 
GACATGGGAC AGGATGCTGA GGACACCAGT GAGGAGCCCC CTGAGAAGGA GGAGCTGGCC 
GGGGTCCAGG AGGCAGAGAC AGCCACAGAC TGCCCTGAAG TTCTTGAGGA GGGATGTGAA 
GAGGCCACGG GTGTCACAGG TGGGGAACAG GTTGACCTCA GTGAACCACC TGACCACGAG 
AAGAAAACCA ACCAAGAAGT GGCAGCCGCC ACCCTGGAGG ACCATGCACA GGATGAGTCC 
GCCGAGGAGA GCTGCCAGAT TGTCCCTTTT GAGAATGACT GCATGGAGGA CTTCGTGACT 
TCCCTCACAG GAAGCCCCTA TGAGTTCTTC CCAACTGAGA GCACCTCTTT TTGCAGCGAG 
AGCTGTTCTC CTCTTTCTGA ATCAGCGAAA GGTTTAGAAT CAGAGCAGGC ACCAAAGCTG 
GGGCTGCGTG CGGAGGAGAA CCCCATGGTG GGGGCTTTGT GTGGCCAGTG TGGCTCCCTA 
CAGGGTGGAG CGGCCGAGGG TCCCGCAGCC CCTGATGTGG TGGTCGTGCT GGAGGAGGAG 
GCCTTGGATG ATGCACTGGC CAACCCCTAT GTGATGGGAG TGGGCCTGCC CGGTCAGGCG 
GCCCCTGGAG AAGGAGGGCA GGCTGCATCG GACGCCCTGG GTGGTTATGG CTCGAAAGAA 
GAATTGAACT GTGAGGCAGA GGGTGGCCTG GTTCCCGCGG ACAGGAAGAA CACCAGCACG 
AGGGTCCGGC CCCACTCTGG GAAGGTGGCC GGCTATGTCC CAGAAACCGT CCCTGAAGAA 
ACCGGACCTG AGGCGGGCTC GTCAGCCCCT GGCATTGGAG GTGCCGCAGA GGAGGTGGGA 
AAGACGCTTT TGTCATTGGA GGGGAAGCCC TTGGAAGCCA GCAGGGCCTT GCCAGCAAAG 
CCCAGGGCCT TTACTTTATA CCCTCGGTCG TTCTCCGTGG AAGGCCGAGA GATTCCAGTG 
TCCGTGTACC AGGAGCCTGA GGGGTCAGGG TTGGATGACC ACAGGATAAA GAGGAAAGAG 
GACAATCTCT CTCTGTCGTG TGTAATTGGC TCCTCTGGGA GTTTCTCCCA GAGAAACCAC 
CTTCCGTCCA GCGGCACCTC CACGCCTTCT TCCATGGTCG ACATCCCACC TCCTTTCGAC 
CTGGCCTGCA TCACCAAGAA GCCCATCACA AAGAGCTCTC CCTCACTCCT GATCGAGAGC 
GACTCCCCGG ACAAGTACAA GAAGAAGAAG TCATCCTTTA AGCGCTTCCT GGCACTGACG 
TTTAAGAAGA AGACGGAGAA CAAATTGCAT GTGGATGTGA ACGTGTCTTC CTCTAGGTCC 
TCTTCAGAGT CCAGCTACCA CGGGCCTTCC AGGATTCTGG AAGTTGACCG GAGAAGCCTC 
AGCAACTCCC CTCAGCTTAA GTCTCGGACT GGGAAGCTCC GGGCTTCTGA ATCCCCCTCC 
TCCCTCATCT TTTATAGAGA TGGCAAGAGG AAAGGTGTCC CCTTCAGCAG GACGGTGTCC 
AGAGTGGAGT CCTTTGAAGA CCGCTCCCGG CCGCCCTTCC TGCCCTTGCC ACTGACCAAG 
CCACGGTCCA TCTCCTTCCC CAGCGCTGAC ACTTCAGACT ATGAGAACAT TCCAGCCATG 
AACTCGGACT ATGAGAATAT CCAGATTCCA CCCCGGAGAC CTGCCAGGGC TGGCGCGTTC 
ACGAAGCTGT TTGAAGATCA GAGCAGAGCC CTGTCCACAG CAAACGAAAA TGATGGCTAC 
GTGGACATGA GCAGCTTCAA CGCCTTTGAG AGCAAACAGC AGAGTGCAGA CCAGGACGCA 
GAAAGCGCCT ACACAGAGCC CTACAAAGTC TGTCCCATCT CGTCGGCAGC CCCCAAAGAG 
GACCTTACGT CGGATGAAGA GCAGAGAAGC TCGGAGGAGG AGGACAGTGC TTCAAGAGAC 
CCCAGTGTCA CCCACAAGGT GGAAGGACAG TCCAGAGCCC TTGTCATCGC ACAGGAACTG 
CTATCTTCAG AGAAAGCATA CGTGGAGATG CTCCAGCACT TAAATCTGGA TTTCCATGGA 
GCTGTCATGA GGGCCTTGGA TGACATGGAC CATGAAGGCA GAGACACATT GGCCCGGGAG 
GAGCTGAGGC AGGGCCTGAG TGAACTCCCA GCCATCCACG ACCTTCATCA AGGCATCCTG 
GAGGAGCTGG AGGAAAGGCT GTCAAATTGG GAGAGCCAGC AGAAGGTAGC TGACGTCTTC 
CTGGCCCGGG AGCAGGGGTT TGATCACCAC GCCACTCACA TCCTGCAGTT CGACAGGTAC 
CTAGGTCTGC TCAGTGAGAA TTGCCTCCAC TCTCCCCGGC TGGCAGCTGC TGTCCGTGAA 
TTTGAGCAGA GTGTACAAGG AGGCAGCCAG ACTGCGAAGC ATCGGCTGCT GCGGGTGGTT 
CAACGCCTCT TCCAGTACCA AGTGCTCCTC ACAGACTATT TAAACAACCT TTGTCCGGAC 
TCCGCCGAGT ACGACAACAC ACAGGGTGCA CTGAGCCTCA TCTCCAAAGT CACAGACCGT 
GCCAACGACA GCATGGAGCA AGGGGAAAAC CTGCAGAAGC TGGTCCACAT TGAGCACAGC 
GTCCGGGGCC AAGGGGATCT CCTCCAGCCA GGAAGGGAGT TTCTGAAGGA AGGGACGCTG 
ATGAAAGTAA CAGGGAAAAA CAGACGGCCC CGGCACCTAT TTCTGATGAA CGATGTGCTC 
CTGTACACCT ATCCCCAGAA GGATGGGAAG TACCGGCTGA AGAACACATT GGCTGTGGCC 
AACATGAAGG TCAGCCGCCC TGTGATGGAG AAAGTGCCCT ACGCTCTAAA GATTGAGACT 
TCCGAGTCCT GCCTGATGCT GTCTGCGAGC TCCTGTGCAG AGAGGGACGA GTGGTATGGC 
TGTCTGAGCA GAGCCCTCCC TGAGGACTAC AAGGCCCAGG CGCTGGCTGC ATTCCACCAT 
AGCGTGGAGA TACGAGAGAG GCTGGGGGTT AGCCTTGGGG AGAGGCCCCC CACCCTGGTG 
CCTGTCACAC ACGTCATGAT GTGCATGAAC TGCGGCTGCG ACTTCTCCCT CACCCTGCGG 
CGTCATCACT GTCACGCCTG TGGCAAGATC GTGTGCCGGA ACTGTTCGCG GAACAAGTAC 
CCGCTGAAGT ACCTGAAGGA CAGGATGGCC AAGGTCTGCG ACGGCTGCTT CGGGGAGCTG 
AAGAAGCGGG GCAGGGCTGT CCCGGGCCTG ATGAGAGAGC GGCCTGTGAG CATGAGCTTC 
CCGCTGTCTT CACCCCGCTT CTCGGGCAGT GCCTTTTCAT CCGTCTTCCA GAGCATTAAC 
CCCTCGACCT TCAAGAAGCA GAAGAAAGTC CCTTCAGCCC TGACAGAGGT GGCTGCCTCT 
GGAGAGGGCT CTGCCATCAG TGGCTATCTC AGCCGGTGTA AGAGGGGCAA GCGGCACTGG 
AAGAAGCTCT GGTTTGTCAT CAAAGGCAAA GTTCTCTACA CCTACATGGC CAGTGAGGAC 
AAAGTGGCCT TGGAGAGTAT GCCTCTGCTA GGCTTCACCA TTGCTCCAGA AAAGGAAGAG 
GGCAGCAGTG AAGTAGGACC TATTTTTCAC CTTTACCACA AGAAAACCCT ATTTTATAGC 
TTCAAAGCAG AAGATACCAA TTCAGCTCAG AGGTGGATCG AGGCCATGGA AGATGCGAGT 
GTGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FGD5 ( NM_001320276.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307205.1 Homo sapiens FYVE, RhoGEF and PH domain containing 5 (FGD5), transcript variant 2, mRNA.

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CloneID	OHu76722
Clone ID Related Accession (Same CDS sequence)	NM_001320276.1
Accession Version	NM_001320276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	FYVE, RhoGEF and PH domain-containing protein 5 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB293373.1, BC144300.1, AC090937.2 and AW589887.1. On Feb 18, 2016 this sequence version replaced XM_011533424.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC144300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCAGGG GTCCGAAGCC CCCCATTGCC CCCAAGCCCA GGCTGACTGC CCCAAACGAG 
TGGAGAGCCA GTGTGTACCT GAATGACAGC TTGAACAAAT GCAGCAACGG GCGGCTGCCC 
TGTGTAGACA GGGGGCTTGA TGAGGGGCCC CGGTCCATCC CAAAGTGCTC TGAGTCGGAG 
ACCGACGAGG ATTACATCGT GGTCCCCAGG GTTCCGCTGA GGGAGGATGA ACCCAAGGAC 
GAGGGCAGTG TGGGGAACAA AGCCCTGGTG TCTCCCGAGT CCTCTGCGGA AGAGGAAGAG 
GAGCGTGAAG AGGGAGGCGA GGCATGTGGC CTGGAGGGTA CAGGAGCTGG TGAGGATTCA 
GTGGCCCCTG CTGCTCCGGG TGCAGGAGCG CTGAGCAGGG AGGGTGAGGA AGGCACAGAC 
CTTGCTCTTG AGGATGAAGG GGAGGGCTGC GCTGATGAGC CAGGGACACT GGAGCAGGTG 
TCCAGAAGTG AGGAGGAAGA GAAGCTAGTG CAGCCACACA GGGAGTGCAG CCTGGAGGAC 
AGTGGGCCTT GGGCTGGAGA GGGGGTCTTC CAGAGCGACC TCCTCCTGCC TCACATCCAT 
GGAGAGGACC AGGAGCCCCC CGACACCCCC GGGGAGGCAG AGGAGGATGA TGAGGAAGGC 
TGTGCCAGCA CAGACCCAGC AGGGGCAGAT GAGGGTTCGG GTCCTGACAG GCCCACGGAG 
GACATGGGAC AGGATGCTGA GGACACCAGT GAGGAGCCCC CTGAGAAGGA GGAGCTGGCC 
GGGGTCCAGG AGGCAGAGAC AGCCACAGAC TGCCCTGAAG TTCTTGAGGA GGGATGTGAA 
GAGGCCACGG GTGTCACAGG TGGGGAACAG GTTGACCTCA GTGAACCACC TGACCACGAG 
AAGAAAACCA ACCAAGAAGT GGCAGCCGCC ACCCTGGAGG ACCATGCACA GGATGAGTCC 
GCCGAGGAGA GCTGCCAGAT TGTCCCTTTT GAGAATGACT GCATGGAGGA CTTCGTGACT 
TCCCTCACAG GAAGCCCCTA TGAGTTCTTC CCAACTGAGA GCACCTCTTT TTGCAGCGAG 
AGCTGTTCTC CTCTTTCTGA ATCAGCGAAA GGTTTAGAAT CAGAGCAGGC ACCAAAGCTG 
GGGCTGCGTG CGGAGGAGAA CCCCATGGTG GGGGCTTTGT GTGGCCAGTG TGGCTCCCTA 
CAGGGTGGAG CGGCCGAGGG TCCCGCAGCC CCTGATGTGG TGGTCGTGCT GGAGGAGGAG 
GCCTTGGATG ATGCACTGGC CAACCCCTAT GTGATGGGAG TGGGCCTGCC CGGTCAGGCG 
GCCCCTGGAG AAGGAGGGCA GGCTGCATCG GACGCCCTGG GTGGTTATGG CTCGAAAGAA 
GAATTGAACT GTGAGGCAGA GGGTGGCCTG GTTCCCGCGG ACAGGAAGAA CACCAGCACG 
AGGGTCCGGC CCCACTCTGG GAAGGTGGCC GGCTATGTCC CAGAAACCGT CCCTGAAGAA 
ACCGGACCTG AGGCGGGCTC GTCAGCCCCT GGCATTGGAG GTGCCGCAGA GGAGGTGGGA 
AAGACGCTTT TGTCATTGGA GGGGAAGCCC TTGGAAGCCA GCAGGGCCTT GCCAGCAAAG 
CCCAGGGCCT TTACTTTATA CCCTCGGTCG TTCTCCGTGG AAGGCCGAGA GATTCCAGTG 
TCCGTGTACC AGGAGCCTGA GGGGTCAGGG TTGGATGACC ACAGGATAAA GAGGAAAGAG 
GACAATCTCT CTCTGTCGTG TGTAATTGGC TCCTCTGGGA GTTTCTCCCA GAGAAACCAC 
CTTCCGTCCA GCGGCACCTC CACGCCTTCT TCCATGGTCG ACATCCCACC TCCTTTCGAC 
CTGGCCTGCA TCACCAAGAA GCCCATCACA AAGAGCTCTC CCTCACTCCT GATCGAGAGC 
GACTCCCCGG ACAAGTACAA GAAGAAGAAG TCATCCTTTA AGCGCTTCCT GGCACTGACG 
TTTAAGAAGA AGACGGAGAA CAAATTGCAT GTGGATGTGA ACGTGTCTTC CTCTAGGTCC 
TCTTCAGAGT CCAGCTACCA CGGGCCTTCC AGGATTCTGG AAGTTGACCG GAGAAGCCTC 
AGCAACTCCC CTCAGCTTAA GTCTCGGACT GGGAAGCTCC GGGCTTCTGA ATCCCCCTCC 
TCCCTCATCT TTTATAGAGA TGGCAAGAGG AAAGGTGTCC CCTTCAGCAG GACGGTGTCC 
AGAGTGGAGT CCTTTGAAGA CCGCTCCCGG CCGCCCTTCC TGCCCTTGCC ACTGACCAAG 
CCACGGTCCA TCTCCTTCCC CAGCGCTGAC ACTTCAGACT ATGAGAACAT TCCAGCCATG 
AACTCGGACT ATGAGAATAT CCAGATTCCA CCCCGGAGAC CTGCCAGGGC TGGCGCGTTC 
ACGAAGCTGT TTGAAGATCA GAGCAGAGCC CTGTCCACAG CAAACGAAAA TGATGGCTAC 
GTGGACATGA GCAGCTTCAA CGCCTTTGAG AGCAAACAGC AGAGTGCAGA CCAGGACGCA 
GAAAGCGCCT ACACAGAGCC CTACAAAGTC TGTCCCATCT CGTCGGCAGC CCCCAAAGAG 
GACCTTACGT CGGATGAAGA GCAGAGAAGC TCGGAGGAGG AGGACAGTGC TTCAAGAGAC 
CCCAGTGTCA CCCACAAGGT GGAAGGACAG TCCAGAGCCC TTGTCATCGC ACAGGAACTG 
CTATCTTCAG AGAAAGCATA CGTGGAGATG CTCCAGCACT TAAATCTGGA TTTCCATGGA 
GCTGTCATGA GGGCCTTGGA TGACATGGAC CATGAAGGCA GAGACACATT GGCCCGGGAG 
GAGCTGAGGC AGGGCCTGAG TGAACTCCCA GCCATCCACG ACCTTCATCA AGGCATCCTG 
GAGGAGCTGG AGGAAAGGCT GTCAAATTGG GAGAGCCAGC AGAAGGTAGC TGACGTCTTC 
CTGGCCCGGG AGCAGGGGTT TGATCACCAC GCCACTCACA TCCTGCAGTT CGACAGGTAC 
CTAGGTCTGC TCAGTGAGAA TTGCCTCCAC TCTCCCCGGC TGGCAGCTGC TGTCCGTGAA 
TTTGAGCAGA GTGTACAAGG AGGCAGCCAG ACTGCGAAGC ATCGGCTGCT GCGGGTGGTT 
CAACGCCTCT TCCAGTACCA AGTGCTCCTC ACAGACTATT TAAACAACCT TTGTCCGGAC 
TCCGCCGAGT ACGACAACAC ACAGGGTGCA CTGAGCCTCA TCTCCAAAGT CACAGACCGT 
GCCAACGACA GCATGGAGCA AGGGGAAAAC CTGCAGAAGC TGGTCCACAT TGAGCACAGC 
GTCCGGGGCC AAGGGGATCT CCTCCAGCCA GGAAGGGAGT TTCTGAAGGA AGGGACGCTG 
ATGAAAGTAA CAGGGAAAAA CAGACGGCCC CGGCACCTAT TTCTGATGAA CGATGTGCTC 
CTGTACACCT ATCCCCAGAA GGATGGGAAG TACCGGCTGA AGAACACATT GGCTGTGGCC 
AACATGAAGG TCAGCCGCCC TGTGATGGAG AAAGTGCCCT ACGCTCTAAA GATTGAGACT 
TCCGAGTCCT GCCTGATGCT GTCTGCGAGC TCCTGTGCAG AGAGGGACGA GTGGTATGGC 
TGTCTGAGCA GAGCCCTCCC TGAGGACTAC AAGGCCCAGG CGCTGGCTGC ATTCCACCAT 
AGCGTGGAGA TACGAGAGAG GCTGGGGGTT AGCCTTGGGG AGAGGCCCCC CACCCTGGTG 
CCTGTCACAC ACGTCATGAT GTGCATGAAC TGCGGCTGCG ACTTCTCCCT CACCCTGCGG 
CGTCATCACT GTCACGCCTG TGGCAAGATC GTGTGCCGGA ACTGTTCGCG GAACAAGTAC 
CCGCTGAAGT ACCTGAAGGA CAGGATGGCC AAGGTCTGCG ACGGCTGCTT CGGGGAGCTG 
AAGAAGCGGG GCAGGGCTGT CCCGGGCCTG ATGAGAGAGC GGCCTGTGAG CATGAGCTTC 
CCGCTGTCTT CACCCCGCTT CTCGGGCAGT GCCTTTTCAT CCGTCTTCCA GAGCATTAAC 
CCCTCGACCT TCAAGAAGCA GAAGAAAGTC CCTTCAGCCC TGACAGAGGA CAAAGTGGCC 
TTGGAGAGTA TGCCTCTGCT AGGCTTCACC ATTGCTCCAG AAAAGGAAGA GGGCAGCAGT 
GAAGTAGGAC CTATTTTTCA CCTTTACCAC AAGAAAACCC TATTTTATAG CTTCAAAGCA 
GAAGATACCA ATTCAGCTCA GAGGTGGATC GAGGCCATGG AAGATGCGAG TGTGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307205.1
CDS	111..4370
Translation	MFRGPKPPIAPKPRLTAPNEWRASVYLNDSLNKCSNGRLPCVDRGLDEGPRSIPKCSESETDEDYIVVPRVPLREDEPKDEGSVGNKALVSPESSAEEEEEREEGGEACGLEGTGAGEDSVAPAAPGAGALSREGEEGTDLALEDEGEGCADEPGTLEQVSRSEEEEKLVQPHRECSLEDSGPWAGEGVFQSDLLLPHIHGEDQEPPDTPGEAEEDDEEGCASTDPAGADEGSGPDRPTEDMGQDAEDTSEEPPEKEELAGVQEAETATDCPEVLEEGCEEATGVTGGEQVDLSEPPDHEKKTNQEVAAATLEDHAQDESAEESCQIVPFENDCMEDFVTSLTGSPYEFFPTESTSFCSESCSPLSESAKGLESEQAPKLGLRAEENPMVGALCGQCGSLQGGAAEGPAAPDVVVVLEEEALDDALANPYVMGVGLPGQAAPGEGGQAASDALGGYGSKEELNCEAEGGLVPADRKNTSTRVRPHSGKVAGYVPETVPEETGPEAGSSAPGIGGAAEEVGKTLLSLEGKPLEASRALPAKPRAFTLYPRSFSVEGREIPVSVYQEPEGSGLDDHRIKRKEDNLSLSCVIGSSGSFSQRNHLPSSGTSTPSSMVDIPPPFDLACITKKPITKSSPSLLIESDSPDKYKKKKSSFKRFLALTFKKKTENKLHVDVNVSSSRSSSESSYHGPSRILEVDRRSLSNSPQLKSRTGKLRASESPSSLIFYRDGKRKGVPFSRTVSRVESFEDRSRPPFLPLPLTKPRSISFPSADTSDYENIPAMNSDYENIQIPPRRPARAGAFTKLFEDQSRALSTANENDGYVDMSSFNAFESKQQSADQDAESAYTEPYKVCPISSAAPKEDLTSDEEQRSSEEEDSASRDPSVTHKVEGQSRALVIAQELLSSEKAYVEMLQHLNLDFHGAVMRALDDMDHEGRDTLAREELRQGLSELPAIHDLHQGILEELEERLSNWESQQKVADVFLAREQGFDHHATHILQFDRYLGLLSENCLHSPRLAAAVREFEQSVQGGSQTAKHRLLRVVQRLFQYQVLLTDYLNNLCPDSAEYDNTQGALSLISKVTDRANDSMEQGENLQKLVHIEHSVRGQGDLLQPGREFLKEGTLMKVTGKNRRPRHLFLMNDVLLYTYPQKDGKYRLKNTLAVANMKVSRPVMEKVPYALKIETSESCLMLSASSCAERDEWYGCLSRALPEDYKAQALAAFHHSVEIRERLGVSLGERPPTLVPVTHVMMCMNCGCDFSLTLRRHHCHACGKIVCRNCSRNKYPLKYLKDRMAKVCDGCFGELKKRGRAVPGLMRERPVSMSFPLSSPRFSGSAFSSVFQSINPSTFKKQKKVPSALTEDKVALESMPLLGFTIAPEKEEGSSEVGPIFHLYHKKTLFYSFKAEDTNSAQRWIEAMEDASVL

Target ORF information:

RefSeq Version	NM_001320276.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FYVE, RhoGEF and PH domain containing 5 (FGD5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320276.1

ORF Insert Sequence:

ATGTTCAGGG GTCCGAAGCC CCCCATTGCC CCCAAGCCCA GGCTGACTGC CCCAAACGAG 
TGGAGAGCCA GTGTGTACCT GAATGACAGC TTGAACAAAT GCAGCAACGG GCGGCTGCCC 
TGTGTAGACA GGGGGCTTGA TGAGGGGCCC CGGTCCATCC CAAAGTGCTC TGAGTCGGAG 
ACCGACGAGG ATTACATCGT GGTCCCCAGG GTTCCGCTGA GGGAGGATGA ACCCAAGGAC 
GAGGGCAGTG TGGGGAACAA AGCCCTGGTG TCTCCCGAGT CCTCTGCGGA AGAGGAAGAG 
GAGCGTGAAG AGGGAGGCGA GGCATGTGGC CTGGAGGGTA CAGGAGCTGG TGAGGATTCA 
GTGGCCCCTG CTGCTCCGGG TGCAGGAGCG CTGAGCAGGG AGGGTGAGGA AGGCACAGAC 
CTTGCTCTTG AGGATGAAGG GGAGGGCTGC GCTGATGAGC CAGGGACACT GGAGCAGGTG 
TCCAGAAGTG AGGAGGAAGA GAAGCTAGTG CAGCCACACA GGGAGTGCAG CCTGGAGGAC 
AGTGGGCCTT GGGCTGGAGA GGGGGTCTTC CAGAGCGACC TCCTCCTGCC TCACATCCAT 
GGAGAGGACC AGGAGCCCCC CGACACCCCC GGGGAGGCAG AGGAGGATGA TGAGGAAGGC 
TGTGCCAGCA CAGACCCAGC AGGGGCAGAT GAGGGTTCGG GTCCTGACAG GCCCACGGAG 
GACATGGGAC AGGATGCTGA GGACACCAGT GAGGAGCCCC CTGAGAAGGA GGAGCTGGCC 
GGGGTCCAGG AGGCAGAGAC AGCCACAGAC TGCCCTGAAG TTCTTGAGGA GGGATGTGAA 
GAGGCCACGG GTGTCACAGG TGGGGAACAG GTTGACCTCA GTGAACCACC TGACCACGAG 
AAGAAAACCA ACCAAGAAGT GGCAGCCGCC ACCCTGGAGG ACCATGCACA GGATGAGTCC 
GCCGAGGAGA GCTGCCAGAT TGTCCCTTTT GAGAATGACT GCATGGAGGA CTTCGTGACT 
TCCCTCACAG GAAGCCCCTA TGAGTTCTTC CCAACTGAGA GCACCTCTTT TTGCAGCGAG 
AGCTGTTCTC CTCTTTCTGA ATCAGCGAAA GGTTTAGAAT CAGAGCAGGC ACCAAAGCTG 
GGGCTGCGTG CGGAGGAGAA CCCCATGGTG GGGGCTTTGT GTGGCCAGTG TGGCTCCCTA 
CAGGGTGGAG CGGCCGAGGG TCCCGCAGCC CCTGATGTGG TGGTCGTGCT GGAGGAGGAG 
GCCTTGGATG ATGCACTGGC CAACCCCTAT GTGATGGGAG TGGGCCTGCC CGGTCAGGCG 
GCCCCTGGAG AAGGAGGGCA GGCTGCATCG GACGCCCTGG GTGGTTATGG CTCGAAAGAA 
GAATTGAACT GTGAGGCAGA GGGTGGCCTG GTTCCCGCGG ACAGGAAGAA CACCAGCACG 
AGGGTCCGGC CCCACTCTGG GAAGGTGGCC GGCTATGTCC CAGAAACCGT CCCTGAAGAA 
ACCGGACCTG AGGCGGGCTC GTCAGCCCCT GGCATTGGAG GTGCCGCAGA GGAGGTGGGA 
AAGACGCTTT TGTCATTGGA GGGGAAGCCC TTGGAAGCCA GCAGGGCCTT GCCAGCAAAG 
CCCAGGGCCT TTACTTTATA CCCTCGGTCG TTCTCCGTGG AAGGCCGAGA GATTCCAGTG 
TCCGTGTACC AGGAGCCTGA GGGGTCAGGG TTGGATGACC ACAGGATAAA GAGGAAAGAG 
GACAATCTCT CTCTGTCGTG TGTAATTGGC TCCTCTGGGA GTTTCTCCCA GAGAAACCAC 
CTTCCGTCCA GCGGCACCTC CACGCCTTCT TCCATGGTCG ACATCCCACC TCCTTTCGAC 
CTGGCCTGCA TCACCAAGAA GCCCATCACA AAGAGCTCTC CCTCACTCCT GATCGAGAGC 
GACTCCCCGG ACAAGTACAA GAAGAAGAAG TCATCCTTTA AGCGCTTCCT GGCACTGACG 
TTTAAGAAGA AGACGGAGAA CAAATTGCAT GTGGATGTGA ACGTGTCTTC CTCTAGGTCC 
TCTTCAGAGT CCAGCTACCA CGGGCCTTCC AGGATTCTGG AAGTTGACCG GAGAAGCCTC 
AGCAACTCCC CTCAGCTTAA GTCTCGGACT GGGAAGCTCC GGGCTTCTGA ATCCCCCTCC 
TCCCTCATCT TTTATAGAGA TGGCAAGAGG AAAGGTGTCC CCTTCAGCAG GACGGTGTCC 
AGAGTGGAGT CCTTTGAAGA CCGCTCCCGG CCGCCCTTCC TGCCCTTGCC ACTGACCAAG 
CCACGGTCCA TCTCCTTCCC CAGCGCTGAC ACTTCAGACT ATGAGAACAT TCCAGCCATG 
AACTCGGACT ATGAGAATAT CCAGATTCCA CCCCGGAGAC CTGCCAGGGC TGGCGCGTTC 
ACGAAGCTGT TTGAAGATCA GAGCAGAGCC CTGTCCACAG CAAACGAAAA TGATGGCTAC 
GTGGACATGA GCAGCTTCAA CGCCTTTGAG AGCAAACAGC AGAGTGCAGA CCAGGACGCA 
GAAAGCGCCT ACACAGAGCC CTACAAAGTC TGTCCCATCT CGTCGGCAGC CCCCAAAGAG 
GACCTTACGT CGGATGAAGA GCAGAGAAGC TCGGAGGAGG AGGACAGTGC TTCAAGAGAC 
CCCAGTGTCA CCCACAAGGT GGAAGGACAG TCCAGAGCCC TTGTCATCGC ACAGGAACTG 
CTATCTTCAG AGAAAGCATA CGTGGAGATG CTCCAGCACT TAAATCTGGA TTTCCATGGA 
GCTGTCATGA GGGCCTTGGA TGACATGGAC CATGAAGGCA GAGACACATT GGCCCGGGAG 
GAGCTGAGGC AGGGCCTGAG TGAACTCCCA GCCATCCACG ACCTTCATCA AGGCATCCTG 
GAGGAGCTGG AGGAAAGGCT GTCAAATTGG GAGAGCCAGC AGAAGGTAGC TGACGTCTTC 
CTGGCCCGGG AGCAGGGGTT TGATCACCAC GCCACTCACA TCCTGCAGTT CGACAGGTAC 
CTAGGTCTGC TCAGTGAGAA TTGCCTCCAC TCTCCCCGGC TGGCAGCTGC TGTCCGTGAA 
TTTGAGCAGA GTGTACAAGG AGGCAGCCAG ACTGCGAAGC ATCGGCTGCT GCGGGTGGTT 
CAACGCCTCT TCCAGTACCA AGTGCTCCTC ACAGACTATT TAAACAACCT TTGTCCGGAC 
TCCGCCGAGT ACGACAACAC ACAGGGTGCA CTGAGCCTCA TCTCCAAAGT CACAGACCGT 
GCCAACGACA GCATGGAGCA AGGGGAAAAC CTGCAGAAGC TGGTCCACAT TGAGCACAGC 
GTCCGGGGCC AAGGGGATCT CCTCCAGCCA GGAAGGGAGT TTCTGAAGGA AGGGACGCTG 
ATGAAAGTAA CAGGGAAAAA CAGACGGCCC CGGCACCTAT TTCTGATGAA CGATGTGCTC 
CTGTACACCT ATCCCCAGAA GGATGGGAAG TACCGGCTGA AGAACACATT GGCTGTGGCC 
AACATGAAGG TCAGCCGCCC TGTGATGGAG AAAGTGCCCT ACGCTCTAAA GATTGAGACT 
TCCGAGTCCT GCCTGATGCT GTCTGCGAGC TCCTGTGCAG AGAGGGACGA GTGGTATGGC 
TGTCTGAGCA GAGCCCTCCC TGAGGACTAC AAGGCCCAGG CGCTGGCTGC ATTCCACCAT 
AGCGTGGAGA TACGAGAGAG GCTGGGGGTT AGCCTTGGGG AGAGGCCCCC CACCCTGGTG 
CCTGTCACAC ACGTCATGAT GTGCATGAAC TGCGGCTGCG ACTTCTCCCT CACCCTGCGG 
CGTCATCACT GTCACGCCTG TGGCAAGATC GTGTGCCGGA ACTGTTCGCG GAACAAGTAC 
CCGCTGAAGT ACCTGAAGGA CAGGATGGCC AAGGTCTGCG ACGGCTGCTT CGGGGAGCTG 
AAGAAGCGGG GCAGGGCTGT CCCGGGCCTG ATGAGAGAGC GGCCTGTGAG CATGAGCTTC 
CCGCTGTCTT CACCCCGCTT CTCGGGCAGT GCCTTTTCAT CCGTCTTCCA GAGCATTAAC 
CCCTCGACCT TCAAGAAGCA GAAGAAAGTC CCTTCAGCCC TGACAGAGGA CAAAGTGGCC 
TTGGAGAGTA TGCCTCTGCT AGGCTTCACC ATTGCTCCAG AAAAGGAAGA GGGCAGCAGT 
GAAGTAGGAC CTATTTTTCA CCTTTACCAC AAGAAAACCC TATTTTATAG CTTCAAAGCA 
GAAGATACCA ATTCAGCTCA GAGGTGGATC GAGGCCATGG AAGATGCGAG TGTGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FGD5 ( XM_011533422.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531724.1 Homo sapiens FYVE, RhoGEF and PH domain containing 5 (FGD5), transcript variant X1, mRNA.

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CloneID	OHu76720
Clone ID Related Accession (Same CDS sequence)	XM_011533422.1
Accession Version	XM_011533422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FYVE, RhoGEF and PH domain-containing protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGGG GTCCGAAGCC CCCCATTGCC CCCAAGCCCA GGCTGACTGC CCCAAACGAG 
TGGAGAGCCA GTGTGTACCT GAATGACAGC TTGAACAAAT GCAGCAACGG GCGGCTGCCC 
TGTGTAGACA GGGGGCTTGA TGAGGGGCCC CGGTCCATCC CAAAGTGCTC TGAGTCGGAG 
ACCGACGAGG ATTACATCGT GGTCCCCAGG GTTCCGCTGA GGGAGGATGA ACCCAAGGAC 
GAGGGCAGTG TGGGGAACAA AGCCCTGGTG TCTCCCGAGT CCTCTGCGGA AGAGGAAGAG 
GAGCGTGAAG AGGGAGGCGA GGCATGTGGC CTGGAGGGTA CAGGAGCTGG TGAGGATTCA 
GTGGCCCCTG CTGCTCCGGG TGCAGGAGCG CTGAGCAGGG AGGGTGAGGA AGGCACAGAC 
CTTGCTCTTG AGGATGAAGG GGAGGGCTGC GCTGATGAGC CAGGGACACT GGAGCAGGTG 
TCCAGAAGTG AGGAGGAAGA GAAGCTAGTG CAGCCACACA GGGAGTGCAG CCTGGAGGAC 
AGTGGGCCTT GGGCTGGAGA GGGGGTCTTC CAGAGCGACC TCCTCCTGCC TCACATCCAT 
GGAGAGGACC AGGAGCCCCC CGACACCCCC GGGGAGGCAG AGGAGGATGA TGAGGAAGGC 
TGTGCCAGCA CAGACCCAGC AGGGGCAGAT GAGGGTTCGG GTCCTGACAG GCCCACGGAG 
GACATGGGAC AGGATGCTGA GGACACCAGT GAGGAGCCCC CTGAGAAGGA GGAGCTGGCC 
GGGGTCCAGG AGGCAGAGAC AGCCACAGAC TGCCCTGAAG TTCTTGAGGA GGGATGTGAA 
GAGGCCACGG GTGTCACAGG TGGGGAACAG GTTGACCTCA GTGAACCACC TGACCACGAG 
AAGAAAACCA ACCAAGAAGT GGCAGCCGCC ACCCTGGAGG ACCATGCACA GGATGAGTCC 
GCCGAGGAGA GCTGCCAGAT TGTCCCTTTT GAGAATGACT GCATGGAGGA CTTCGTGACT 
TCCCTCACAG GAAGCCCCTA TGAGTTCTTC CCAACTGAGA GCACCTCTTT TTGCAGCGAG 
AGCTGTTCTC CTCTTTCTGA ATCAGCGAAA GGTTTAGAAT CAGAGCAGGC ACCAAAGCTG 
GGGCTGCGTG CGGAGGAGAA CCCCATGGTG GGGGCTTTGT GTGGCCAGTG TGGCTCCCTA 
CAGGGTGGAG CGGCCGAGGG TCCCGCAGCC CCTGATGTGG TGGTCGTGCT GGAGGAGGAG 
GCCTTGGATG ATGCACTGGC CAACCCCTAT GTGATGGGAG TGGGCCTGCC CGGTCAGGCG 
GCCCCTGGAG AAGGAGGGCA GGCTGCATCG GACGCCCTGG GTGGTTATGG CTCGAAAGAA 
GAATTGAACT GTGAGGCAGA GGGTGGCCTG GTTCCCGCGG ACAGGAAGAA CACCAGCACG 
AGGGTCCGGC CCCACTCTGG GAAGGTGGCC GGCTATGTCC CAGAAACCGT CCCTGAAGAA 
ACCGGACCTG AGGCGGGCTC GTCAGCCCCT GGCATTGGAG GTGCCGCAGA GGAGGTGGGA 
AAGACGCTTT TGTCATTGGA GGGGAAGCCC TTGGAAGCCA GCAGGGCCTT GCCAGCAAAG 
CCCAGGGCCT TTACTTTATA CCCTCGGTCG TTCTCCGTGG AAGGCCGAGA GATTCCAGTG 
TCCGTGTACC AGGAGCCTGA GGGGTCAGGG TTGGATGACC ACAGGATAAA GAGGAAAGAG 
GACAATCTCT CTCTGTCGTG TGTAATTGGC TCCTCTGGGA GTTTCTCCCA GAGAAACCAC 
CTTCCGTCCA GCGGCACCTC CACGCCTTCT TCCATGGTCG ACATCCCACC TCCTTTCGAC 
CTGGCCTGCA TCACCAAGAA GCCCATCACA AAGAGCTCTC CCTCACTCCT GATCGAGAGC 
GACTCCCCGG ACAAGTACAA GAAGAAGAAG TCATCCTTTA AGCGCTTCCT GGCACTGACG 
TTTAAGAAGA AGACGGAGAA CAAATTGCAT GTGGATGTGA ACGTGTCTTC CTCTAGGTCC 
TCTTCAGAGT CCAGCTACCA CGGGCCTTCC AGGATTCTGG AAGTTGACCG GAGAAGCCTC 
AGCAACTCCC CTCAGCTTAA GTCTCGGACT GGGAAGCTCC GGGCTTCTGA ATCCCCCTCC 
TCCCTCATCT TTTATAGAGA TGGCAAGAGG AAAGGTGTCC CCTTCAGCAG GACGGTGTCC 
AGAGTGGAGT CCTTTGAAGA CCGCTCCCGG CCGCCCTTCC TGCCCTTGCC ACTGACCAAG 
CCACGGTCCA TCTCCTTCCC CAGCGCTGAC ACTTCAGACT ATGAGAACAT TCCAGCCATG 
AACTCGGACT ATGAGAATAT CCAGATTCCA CCCCGGAGAC CTGCCAGGGC TGGCGCGTTC 
ACGAAGCTGT TTGAAGATCA GAGCAGAGCC CTGTCCACAG CAAACGAAAA TGATGGCTAC 
GTGGACATGA GCAGCTTCAA CGCCTTTGAG AGCAAACAGC AGAGTGCAGA CCAGGACGCA 
GAAAGCGCCT ACACAGAGCC CTACAAAGTC TGTCCCATCT CGTCGGCAGC CCCCAAAGAG 
GACCTTACGT CGGATGAAGA GCAGAGAAGC TCGGAGGAGG AGGACAGTGC TTCAAGAGAC 
CCCAGTGTCA CCCACAAGGT GGAAGGACAG TCCAGAGCCC TTGTCATCGC ACAGGAACTG 
CTATCTTCAG AGAAAGCATA CGTGGAGATG CTCCAGCACT TAAATCTGGA TTTCCATGGA 
GCTGTCATGA GGGCCTTGGA TGACATGGAC CATGAAGGCA GAGACACATT GGCCCGGGAG 
GAGCTGAGGC AGGGCCTGAG TGAACTCCCA GCCATCCACG ACCTTCATCA AGGCATCCTG 
GAGGAGCTGG AGGAAAGGCT GTCAAATTGG GAGAGCCAGC AGAAGGTAGC TGACGTCTTC 
CTGGCCCGGG AGCAGGGGTT TGATCACCAC GCCACTCACA TCCTGCAGTT CGACAGGTAC 
CTAGGTCTGC TCAGTGAGAA TTGCCTCCAC TCTCCCCGGC TGGCAGCTGC TGTCCGTGAA 
TTTGAGCAGA GTGTACAAGG AGGCAGCCAG ACTGCGAAGC ATCGGCTGCT GCGGGTGGTT 
CAACGCCTCT TCCAGTACCA AGTGCTCCTC ACAGACTATT TAAACAACCT TTGTCCGGAC 
TCCGCCGAGT ACGACAACAC ACAGGGTGCA CTGAGCCTCA TCTCCAAAGT CACAGACCGT 
GCCAACGACA GCATGGAGCA AGGGGAAAAC CTGCAGAAGC TGGTCCACAT TGAGCACAGC 
GTCCGGGGCC AAGGGGATCT CCTCCAGCCA GGAAGGGAGT TTCTGAAGGA AGGGACGCTG 
ATGAAAGTAA CAGGGAAAAA CAGACGGCCC CGGCACCTAT TTCTGATGAA CGATGTGCTC 
CTGTACACCT ATCCCCAGAA GGATGGGAAG TACCGGCTGA AGAACACATT GGCTGTGGCC 
AACATGAAGG TCAGCCGCCC TGTGATGGAG AAAGTGCCCT ACGCTCTAAA GATTGAGACT 
TCCGAGTCCT GCCTGATGCT GTCTGCGAGC TCCTGTGCAG AGAGGGACGA GTGGTATGGC 
TGTCTGAGCA GAGCCCTCCC TGAGGACTAC AAGGCCCAGG CGCTGGCTGC ATTCCACCAT 
AGCGTGGAGA TACGAGAGAG GCTGGGGGTT AGCCTTGGGG AGAGGCCCCC CACCCTGGTG 
CCTGTCACAC ACGTCATGAT GTGCATGAAC TGCGGCTGCG ACTTCTCCCT CACCCTGCGG 
CGTCATCACT GTCACGCCTG TGGCAAGATC GTGTGCCGGA ACTGTTCGCG GAACAAGTAC 
CCGCTGAAGT ACCTGAAGGA CAGGATGGCC AAGGTCTGCG ACGGCTGCTT CGGGGAGCTG 
AAGAAGCGGG GCAGGGCTGT CCCGGGCCTG ATGAGAGTTA CAGAGCGGCC TGTGAGCATG 
AGCTTCCCGC TGTCTTCACC CCGCTTCTCG GGCAGTGCCT TTTCATCCGT CTTCCAGAGC 
ATTAACCCCT CGACCTTCAA GAAGCAGAAG AAAGTCCCTT CAGCCCTGAC AGAGGTGGCT 
GCCTCTGGAG AGGGCTCTGC CATCAGTGGC TATCTCAGCC GGTGTAAGAG GGGCAAGCGG 
CACTGGAAGA AGCTCTGGTT TGTCATCAAA GGCAAAGTTC TCTACACCTA CATGGCCAGT 
GAGGACAAAG TGGCCTTGGA GAGTATGCCT CTGCTAGGCT TCACCATTGC TCCAGAAAAG 
GAAGAGGGCA GCAGTGAAGT AGGACCTATT TTTCACCTTT ACCACAAGAA AACCCTATTT 
TATAGCTTCA AAGCAGAAGA TACCAATTCA GCTCAGAGGT GGATCGAGGC CATGGAAGAT 
GCGAGTGTGT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531724.1
CDS	111..4505
Translation	MFRGPKPPIAPKPRLTAPNEWRASVYLNDSLNKCSNGRLPCVDRGLDEGPRSIPKCSESETDEDYIVVPRVPLREDEPKDEGSVGNKALVSPESSAEEEEEREEGGEACGLEGTGAGEDSVAPAAPGAGALSREGEEGTDLALEDEGEGCADEPGTLEQVSRSEEEEKLVQPHRECSLEDSGPWAGEGVFQSDLLLPHIHGEDQEPPDTPGEAEEDDEEGCASTDPAGADEGSGPDRPTEDMGQDAEDTSEEPPEKEELAGVQEAETATDCPEVLEEGCEEATGVTGGEQVDLSEPPDHEKKTNQEVAAATLEDHAQDESAEESCQIVPFENDCMEDFVTSLTGSPYEFFPTESTSFCSESCSPLSESAKGLESEQAPKLGLRAEENPMVGALCGQCGSLQGGAAEGPAAPDVVVVLEEEALDDALANPYVMGVGLPGQAAPGEGGQAASDALGGYGSKEELNCEAEGGLVPADRKNTSTRVRPHSGKVAGYVPETVPEETGPEAGSSAPGIGGAAEEVGKTLLSLEGKPLEASRALPAKPRAFTLYPRSFSVEGREIPVSVYQEPEGSGLDDHRIKRKEDNLSLSCVIGSSGSFSQRNHLPSSGTSTPSSMVDIPPPFDLACITKKPITKSSPSLLIESDSPDKYKKKKSSFKRFLALTFKKKTENKLHVDVNVSSSRSSSESSYHGPSRILEVDRRSLSNSPQLKSRTGKLRASESPSSLIFYRDGKRKGVPFSRTVSRVESFEDRSRPPFLPLPLTKPRSISFPSADTSDYENIPAMNSDYENIQIPPRRPARAGAFTKLFEDQSRALSTANENDGYVDMSSFNAFESKQQSADQDAESAYTEPYKVCPISSAAPKEDLTSDEEQRSSEEEDSASRDPSVTHKVEGQSRALVIAQELLSSEKAYVEMLQHLNLDFHGAVMRALDDMDHEGRDTLAREELRQGLSELPAIHDLHQGILEELEERLSNWESQQKVADVFLAREQGFDHHATHILQFDRYLGLLSENCLHSPRLAAAVREFEQSVQGGSQTAKHRLLRVVQRLFQYQVLLTDYLNNLCPDSAEYDNTQGALSLISKVTDRANDSMEQGENLQKLVHIEHSVRGQGDLLQPGREFLKEGTLMKVTGKNRRPRHLFLMNDVLLYTYPQKDGKYRLKNTLAVANMKVSRPVMEKVPYALKIETSESCLMLSASSCAERDEWYGCLSRALPEDYKAQALAAFHHSVEIRERLGVSLGERPPTLVPVTHVMMCMNCGCDFSLTLRRHHCHACGKIVCRNCSRNKYPLKYLKDRMAKVCDGCFGELKKRGRAVPGLMRVTERPVSMSFPLSSPRFSGSAFSSVFQSINPSTFKKQKKVPSALTEVAASGEGSAISGYLSRCKRGKRHWKKLWFVIKGKVLYTYMASEDKVALESMPLLGFTIAPEKEEGSSEVGPIFHLYHKKTLFYSFKAEDTNSAQRWIEAMEDASVL

Target ORF information:

RefSeq Version	XM_011533422.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FYVE, RhoGEF and PH domain containing 5 (FGD5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533422.1

ORF Insert Sequence:

ATGTTCAGGG GTCCGAAGCC CCCCATTGCC CCCAAGCCCA GGCTGACTGC CCCAAACGAG 
TGGAGAGCCA GTGTGTACCT GAATGACAGC TTGAACAAAT GCAGCAACGG GCGGCTGCCC 
TGTGTAGACA GGGGGCTTGA TGAGGGGCCC CGGTCCATCC CAAAGTGCTC TGAGTCGGAG 
ACCGACGAGG ATTACATCGT GGTCCCCAGG GTTCCGCTGA GGGAGGATGA ACCCAAGGAC 
GAGGGCAGTG TGGGGAACAA AGCCCTGGTG TCTCCCGAGT CCTCTGCGGA AGAGGAAGAG 
GAGCGTGAAG AGGGAGGCGA GGCATGTGGC CTGGAGGGTA CAGGAGCTGG TGAGGATTCA 
GTGGCCCCTG CTGCTCCGGG TGCAGGAGCG CTGAGCAGGG AGGGTGAGGA AGGCACAGAC 
CTTGCTCTTG AGGATGAAGG GGAGGGCTGC GCTGATGAGC CAGGGACACT GGAGCAGGTG 
TCCAGAAGTG AGGAGGAAGA GAAGCTAGTG CAGCCACACA GGGAGTGCAG CCTGGAGGAC 
AGTGGGCCTT GGGCTGGAGA GGGGGTCTTC CAGAGCGACC TCCTCCTGCC TCACATCCAT 
GGAGAGGACC AGGAGCCCCC CGACACCCCC GGGGAGGCAG AGGAGGATGA TGAGGAAGGC 
TGTGCCAGCA CAGACCCAGC AGGGGCAGAT GAGGGTTCGG GTCCTGACAG GCCCACGGAG 
GACATGGGAC AGGATGCTGA GGACACCAGT GAGGAGCCCC CTGAGAAGGA GGAGCTGGCC 
GGGGTCCAGG AGGCAGAGAC AGCCACAGAC TGCCCTGAAG TTCTTGAGGA GGGATGTGAA 
GAGGCCACGG GTGTCACAGG TGGGGAACAG GTTGACCTCA GTGAACCACC TGACCACGAG 
AAGAAAACCA ACCAAGAAGT GGCAGCCGCC ACCCTGGAGG ACCATGCACA GGATGAGTCC 
GCCGAGGAGA GCTGCCAGAT TGTCCCTTTT GAGAATGACT GCATGGAGGA CTTCGTGACT 
TCCCTCACAG GAAGCCCCTA TGAGTTCTTC CCAACTGAGA GCACCTCTTT TTGCAGCGAG 
AGCTGTTCTC CTCTTTCTGA ATCAGCGAAA GGTTTAGAAT CAGAGCAGGC ACCAAAGCTG 
GGGCTGCGTG CGGAGGAGAA CCCCATGGTG GGGGCTTTGT GTGGCCAGTG TGGCTCCCTA 
CAGGGTGGAG CGGCCGAGGG TCCCGCAGCC CCTGATGTGG TGGTCGTGCT GGAGGAGGAG 
GCCTTGGATG ATGCACTGGC CAACCCCTAT GTGATGGGAG TGGGCCTGCC CGGTCAGGCG 
GCCCCTGGAG AAGGAGGGCA GGCTGCATCG GACGCCCTGG GTGGTTATGG CTCGAAAGAA 
GAATTGAACT GTGAGGCAGA GGGTGGCCTG GTTCCCGCGG ACAGGAAGAA CACCAGCACG 
AGGGTCCGGC CCCACTCTGG GAAGGTGGCC GGCTATGTCC CAGAAACCGT CCCTGAAGAA 
ACCGGACCTG AGGCGGGCTC GTCAGCCCCT GGCATTGGAG GTGCCGCAGA GGAGGTGGGA 
AAGACGCTTT TGTCATTGGA GGGGAAGCCC TTGGAAGCCA GCAGGGCCTT GCCAGCAAAG 
CCCAGGGCCT TTACTTTATA CCCTCGGTCG TTCTCCGTGG AAGGCCGAGA GATTCCAGTG 
TCCGTGTACC AGGAGCCTGA GGGGTCAGGG TTGGATGACC ACAGGATAAA GAGGAAAGAG 
GACAATCTCT CTCTGTCGTG TGTAATTGGC TCCTCTGGGA GTTTCTCCCA GAGAAACCAC 
CTTCCGTCCA GCGGCACCTC CACGCCTTCT TCCATGGTCG ACATCCCACC TCCTTTCGAC 
CTGGCCTGCA TCACCAAGAA GCCCATCACA AAGAGCTCTC CCTCACTCCT GATCGAGAGC 
GACTCCCCGG ACAAGTACAA GAAGAAGAAG TCATCCTTTA AGCGCTTCCT GGCACTGACG 
TTTAAGAAGA AGACGGAGAA CAAATTGCAT GTGGATGTGA ACGTGTCTTC CTCTAGGTCC 
TCTTCAGAGT CCAGCTACCA CGGGCCTTCC AGGATTCTGG AAGTTGACCG GAGAAGCCTC 
AGCAACTCCC CTCAGCTTAA GTCTCGGACT GGGAAGCTCC GGGCTTCTGA ATCCCCCTCC 
TCCCTCATCT TTTATAGAGA TGGCAAGAGG AAAGGTGTCC CCTTCAGCAG GACGGTGTCC 
AGAGTGGAGT CCTTTGAAGA CCGCTCCCGG CCGCCCTTCC TGCCCTTGCC ACTGACCAAG 
CCACGGTCCA TCTCCTTCCC CAGCGCTGAC ACTTCAGACT ATGAGAACAT TCCAGCCATG 
AACTCGGACT ATGAGAATAT CCAGATTCCA CCCCGGAGAC CTGCCAGGGC TGGCGCGTTC 
ACGAAGCTGT TTGAAGATCA GAGCAGAGCC CTGTCCACAG CAAACGAAAA TGATGGCTAC 
GTGGACATGA GCAGCTTCAA CGCCTTTGAG AGCAAACAGC AGAGTGCAGA CCAGGACGCA 
GAAAGCGCCT ACACAGAGCC CTACAAAGTC TGTCCCATCT CGTCGGCAGC CCCCAAAGAG 
GACCTTACGT CGGATGAAGA GCAGAGAAGC TCGGAGGAGG AGGACAGTGC TTCAAGAGAC 
CCCAGTGTCA CCCACAAGGT GGAAGGACAG TCCAGAGCCC TTGTCATCGC ACAGGAACTG 
CTATCTTCAG AGAAAGCATA CGTGGAGATG CTCCAGCACT TAAATCTGGA TTTCCATGGA 
GCTGTCATGA GGGCCTTGGA TGACATGGAC CATGAAGGCA GAGACACATT GGCCCGGGAG 
GAGCTGAGGC AGGGCCTGAG TGAACTCCCA GCCATCCACG ACCTTCATCA AGGCATCCTG 
GAGGAGCTGG AGGAAAGGCT GTCAAATTGG GAGAGCCAGC AGAAGGTAGC TGACGTCTTC 
CTGGCCCGGG AGCAGGGGTT TGATCACCAC GCCACTCACA TCCTGCAGTT CGACAGGTAC 
CTAGGTCTGC TCAGTGAGAA TTGCCTCCAC TCTCCCCGGC TGGCAGCTGC TGTCCGTGAA 
TTTGAGCAGA GTGTACAAGG AGGCAGCCAG ACTGCGAAGC ATCGGCTGCT GCGGGTGGTT 
CAACGCCTCT TCCAGTACCA AGTGCTCCTC ACAGACTATT TAAACAACCT TTGTCCGGAC 
TCCGCCGAGT ACGACAACAC ACAGGGTGCA CTGAGCCTCA TCTCCAAAGT CACAGACCGT 
GCCAACGACA GCATGGAGCA AGGGGAAAAC CTGCAGAAGC TGGTCCACAT TGAGCACAGC 
GTCCGGGGCC AAGGGGATCT CCTCCAGCCA GGAAGGGAGT TTCTGAAGGA AGGGACGCTG 
ATGAAAGTAA CAGGGAAAAA CAGACGGCCC CGGCACCTAT TTCTGATGAA CGATGTGCTC 
CTGTACACCT ATCCCCAGAA GGATGGGAAG TACCGGCTGA AGAACACATT GGCTGTGGCC 
AACATGAAGG TCAGCCGCCC TGTGATGGAG AAAGTGCCCT ACGCTCTAAA GATTGAGACT 
TCCGAGTCCT GCCTGATGCT GTCTGCGAGC TCCTGTGCAG AGAGGGACGA GTGGTATGGC 
TGTCTGAGCA GAGCCCTCCC TGAGGACTAC AAGGCCCAGG CGCTGGCTGC ATTCCACCAT 
AGCGTGGAGA TACGAGAGAG GCTGGGGGTT AGCCTTGGGG AGAGGCCCCC CACCCTGGTG 
CCTGTCACAC ACGTCATGAT GTGCATGAAC TGCGGCTGCG ACTTCTCCCT CACCCTGCGG 
CGTCATCACT GTCACGCCTG TGGCAAGATC GTGTGCCGGA ACTGTTCGCG GAACAAGTAC 
CCGCTGAAGT ACCTGAAGGA CAGGATGGCC AAGGTCTGCG ACGGCTGCTT CGGGGAGCTG 
AAGAAGCGGG GCAGGGCTGT CCCGGGCCTG ATGAGAGTTA CAGAGCGGCC TGTGAGCATG 
AGCTTCCCGC TGTCTTCACC CCGCTTCTCG GGCAGTGCCT TTTCATCCGT CTTCCAGAGC 
ATTAACCCCT CGACCTTCAA GAAGCAGAAG AAAGTCCCTT CAGCCCTGAC AGAGGTGGCT 
GCCTCTGGAG AGGGCTCTGC CATCAGTGGC TATCTCAGCC GGTGTAAGAG GGGCAAGCGG 
CACTGGAAGA AGCTCTGGTT TGTCATCAAA GGCAAAGTTC TCTACACCTA CATGGCCAGT 
GAGGACAAAG TGGCCTTGGA GAGTATGCCT CTGCTAGGCT TCACCATTGC TCCAGAAAAG 
GAAGAGGGCA GCAGTGAAGT AGGACCTATT TTTCACCTTT ACCACAAGAA AACCCTATTT 
TATAGCTTCA AAGCAGAAGA TACCAATTCA GCTCAGAGGT GGATCGAGGC CATGGAAGAT 
GCGAGTGTGT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FGD5 ( XM_011533423.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531725.1 Homo sapiens FYVE, RhoGEF and PH domain containing 5 (FGD5), transcript variant X2, mRNA.

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CloneID	OHu76721
Clone ID Related Accession (Same CDS sequence)	XM_011533423.1
Accession Version	XM_011533423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FYVE, RhoGEF and PH domain-containing protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGGG GTCCGAAGCC CCCCATTGCC CCCAAGCCCA GGCTGACTGC CCCAAACGAG 
TGGAGAGCCA GTGTGTACCT GAATGACAGC TTGAACAAAT GCAGCAACGG GCGGCTGCCC 
TGTGTAGACA GGGGGCTTGA TGAGGGGCCC CGGTCCATCC CAAAGTGCTC TGAGTCGGAG 
ACCGACGAGG ATTACATCGT GGTCCCCAGG GTTCCGCTGA GGGAGGATGA ACCCAAGGAC 
GAGGGCAGTG TGGGGAACAA AGCCCTGGTG TCTCCCGAGT CCTCTGCGGA AGAGGAAGAG 
GAGCGTGAAG AGGGAGGCGA GGCATGTGGC CTGGAGGGTA CAGGAGCTGG TGAGGATTCA 
GTGGCCCCTG CTGCTCCGGG TGCAGGAGCG CTGAGCAGGG AGGGTGAGGA AGGCACAGAC 
CTTGCTCTTG AGGATGAAGG GGAGGGCTGC GCTGATGAGC CAGGGACACT GGAGCAGGTG 
TCCAGAAGTG AGGAGGAAGA GAAGCTAGTG CAGCCACACA GGGAGTGCAG CCTGGAGGAC 
AGTGGGCCTT GGGCTGGAGA GGGGGTCTTC CAGAGCGACC TCCTCCTGCC TCACATCCAT 
GGAGAGGACC AGGAGCCCCC CGACACCCCC GGGGAGGCAG AGGAGGATGA TGAGGAAGGC 
TGTGCCAGCA CAGACCCAGC AGGGGCAGAT GAGGGTTCGG GTCCTGACAG GCCCACGGAG 
GACATGGGAC AGGATGCTGA GGACACCAGT GAGGAGCCCC CTGAGAAGGA GGAGCTGGCC 
GGGGTCCAGG AGGCAGAGAC AGCCACAGAC TGCCCTGAAG TTCTTGAGGA GGGATGTGAA 
GAGGCCACGG GTGTCACAGG TGGGGAACAG GTTGACCTCA GTGAACCACC TGACCACGAG 
AAGAAAACCA ACCAAGAAGT GGCAGCCGCC ACCCTGGAGG ACCATGCACA GGATGAGTCC 
GCCGAGGAGA GCTGCCAGAT TGTCCCTTTT GAGAATGACT GCATGGAGGA CTTCGTGACT 
TCCCTCACAG GAAGCCCCTA TGAGTTCTTC CCAACTGAGA GCACCTCTTT TTGCAGCGAG 
AGCTGTTCTC CTCTTTCTGA ATCAGCGAAA GGTTTAGAAT CAGAGCAGGC ACCAAAGCTG 
GGGCTGCGTG CGGAGGAGAA CCCCATGGTG GGGGCTTTGT GTGGCCAGTG TGGCTCCCTA 
CAGGGTGGAG CGGCCGAGGG TCCCGCAGCC CCTGATGTGG TGGTCGTGCT GGAGGAGGAG 
GCCTTGGATG ATGCACTGGC CAACCCCTAT GTGATGGGAG TGGGCCTGCC CGGTCAGGCG 
GCCCCTGGAG AAGGAGGGCA GGCTGCATCG GACGCCCTGG GTGGTTATGG CTCGAAAGAA 
GAATTGAACT GTGAGGCAGA GGGTGGCCTG GTTCCCGCGG ACAGGAAGAA CACCAGCACG 
AGGGTCCGGC CCCACTCTGG GAAGGTGGCC GGCTATGTCC CAGAAACCGT CCCTGAAGAA 
ACCGGACCTG AGGCGGGCTC GTCAGCCCCT GGCATTGGAG GTGCCGCAGA GGAGGTGGGA 
AAGACGCTTT TGTCATTGGA GGGGAAGCCC TTGGAAGCCA GCAGGGCCTT GCCAGCAAAG 
CCCAGGGCCT TTACTTTATA CCCTCGGTCG TTCTCCGTGG AAGGCCGAGA GATTCCAGTG 
TCCGTGTACC AGGAGCCTGA GGGGTCAGGG TTGGATGACC ACAGGATAAA GAGGAAAGAG 
GACAATCTCT CTCTGTCGTG TGTAATTGGC TCCTCTGGGA GTTTCTCCCA GAGAAACCAC 
CTTCCGTCCA GCGGCACCTC CACGCCTTCT TCCATGGTCG ACATCCCACC TCCTTTCGAC 
CTGGCCTGCA TCACCAAGAA GCCCATCACA AAGAGCTCTC CCTCACTCCT GATCGAGAGC 
GACTCCCCGG ACAAGTACAA GAAGAAGAAG TCATCCTTTA AGCGCTTCCT GGCACTGACG 
TTTAAGAAGA AGACGGAGAA CAAATTGCAT GTGGATGTGA ACGTGTCTTC CTCTAGGTCC 
TCTTCAGAGT CCAGCTACCA CGGGCCTTCC AGGATTCTGG AAGTTGACCG GAGAAGCCTC 
AGCAACTCCC CTCAGCTTAA GTCTCGGACT GGGAAGCTCC GGGCTTCTGA ATCCCCCTCC 
TCCCTCATCT TTTATAGAGA TGGCAAGAGG AAAGGTGTCC CCTTCAGCAG GACGGTGTCC 
AGAGTGGAGT CCTTTGAAGA CCGCTCCCGG CCGCCCTTCC TGCCCTTGCC ACTGACCAAG 
CCACGGTCCA TCTCCTTCCC CAGCGCTGAC ACTTCAGACT ATGAGAACAT TCCAGCCATG 
AACTCGGACT ATGAGAATAT CCAGATTCCA CCCCGGAGAC CTGCCAGGGC TGGCGCGTTC 
ACGAAGCTGT TTGAAGATCA GAGCAGAGCC CTGTCCACAG CAAACGAAAA TGATGGCTAC 
GTGGACATGA GCAGCTTCAA CGCCTTTGAG AGCAAACAGC AGAGTGCAGA CCAGGACGCA 
GAAAGCGCCT ACACAGAGCC CTACAAAGTC TGTCCCATCT CGTCGGCAGC CCCCAAAGAG 
GACCTTACGT CGGATGAAGA GCAGAGAAGC TCGGAGGAGG AGGACAGTGC TTCAAGAGAC 
CCCAGTGTCA CCCACAAGGT GGAAGGACAG TCCAGAGCCC TTGTCATCGC ACAGGAACTG 
CTATCTTCAG AGAAAGCATA CGTGGAGATG CTCCAGCACT TAAATCTGGA TTTCCATGGA 
GCTGTCATGA GGGCCTTGGA TGACATGGAC CATGAAGGCA GAGACACATT GGCCCGGGAG 
GAGCTGAGGC AGGGCCTGAG TGAACTCCCA GCCATCCACG ACCTTCATCA AGGCATCCTG 
GAGGAGCTGG AGGAAAGGCT GTCAAATTGG GAGAGCCAGC AGAAGGTAGC TGACGTCTTC 
CTGGCCCGGG AGCAGGGGTT TGATCACCAC GCCACTCACA TCCTGCAGTT CGACAGGTAC 
CTAGGTCTGC TCAGTGAGAA TTGCCTCCAC TCTCCCCGGC TGGCAGCTGC TGTCCGTGAA 
TTTGAGCAGA GTGTACAAGG AGGCAGCCAG ACTGCGAAGC ATCGGCTGCT GCGGGTGGTT 
CAACGCCTCT TCCAGTACCA AGTGCTCCTC ACAGACTATT TAAACAACCT TTGTCCGGAC 
TCCGCCGAGT ACGACAACAC ACAGGGTGCA CTGAGCCTCA TCTCCAAAGT CACAGACCGT 
GCCAACGACA GCATGGAGCA AGGGGAAAAC CTGCAGAAGC TGGTCCACAT TGAGCACAGC 
GTCCGGGGCC AAGGGGATCT CCTCCAGCCA GGAAGGGAGT TTCTGAAGGA AGGGACGCTG 
ATGAAAGTAA CAGGGAAAAA CAGACGGCCC CGGCACCTAT TTCTGATGAA CGATGTGCTC 
CTGTACACCT ATCCCCAGAA GGATGGGAAG TACCGGCTGA AGAACACATT GGCTGTGGCC 
AACATGAAGG TCAGCCGCCC TGTGATGGAG AAAGTGCCCT ACGCTCTAAA GATTGAGACT 
TCCGAGTCCT GCCTGATGCT GTCTGCGAGC TCCTGTGCAG AGAGGGACGA GTGGTATGGC 
TGTCTGAGCA GAGCCCTCCC TGAGGACTAC AAGGCCCAGG CGCTGGCTGC ATTCCACCAT 
AGCGTGGAGA TACGAGAGAG GCTGGGGGTT AGCCTTGGGG AGAGGCCCCC CACCCTGGTG 
CCTGTCACAC ACGTCATGAT GTGCATGAAC TGCGGCTGCG ACTTCTCCCT CACCCTGCGG 
CGTCATCACT GTCACGCCTG TGGCAAGATC GTGTGCCGGA ACTGTTCGCG GAACAAGTAC 
CCGCTGAAGT ACCTGAAGGA CAGGATGGCC AAGGTCTGCG ACGGCTGCTT CGGGGAGCTG 
AAGAAGCGGG GCAGGGCTGT CCCGGGCCTG ATGAGAGTTA CAGAGCGGCC TGTGAGCATG 
AGCTTCCCGC TGTCTTCACC CCGCTTCTCG GGCAGTGCCT TTTCATCCGT CTTCCAGAGC 
ATTAACCCCT CGACCTTCAA GAAGCAGAAG AAAGTCCCTT CAGCCCTGAC AGAGGACAAA 
GTGGCCTTGG AGAGTATGCC TCTGCTAGGC TTCACCATTG CTCCAGAAAA GGAAGAGGGC 
AGCAGTGAAG TAGGACCTAT TTTTCACCTT TACCACAAGA AAACCCTATT TTATAGCTTC 
AAAGCAGAAG ATACCAATTC AGCTCAGAGG TGGATCGAGG CCATGGAAGA TGCGAGTGTG 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531725.1
CDS	111..4376
Translation	MFRGPKPPIAPKPRLTAPNEWRASVYLNDSLNKCSNGRLPCVDRGLDEGPRSIPKCSESETDEDYIVVPRVPLREDEPKDEGSVGNKALVSPESSAEEEEEREEGGEACGLEGTGAGEDSVAPAAPGAGALSREGEEGTDLALEDEGEGCADEPGTLEQVSRSEEEEKLVQPHRECSLEDSGPWAGEGVFQSDLLLPHIHGEDQEPPDTPGEAEEDDEEGCASTDPAGADEGSGPDRPTEDMGQDAEDTSEEPPEKEELAGVQEAETATDCPEVLEEGCEEATGVTGGEQVDLSEPPDHEKKTNQEVAAATLEDHAQDESAEESCQIVPFENDCMEDFVTSLTGSPYEFFPTESTSFCSESCSPLSESAKGLESEQAPKLGLRAEENPMVGALCGQCGSLQGGAAEGPAAPDVVVVLEEEALDDALANPYVMGVGLPGQAAPGEGGQAASDALGGYGSKEELNCEAEGGLVPADRKNTSTRVRPHSGKVAGYVPETVPEETGPEAGSSAPGIGGAAEEVGKTLLSLEGKPLEASRALPAKPRAFTLYPRSFSVEGREIPVSVYQEPEGSGLDDHRIKRKEDNLSLSCVIGSSGSFSQRNHLPSSGTSTPSSMVDIPPPFDLACITKKPITKSSPSLLIESDSPDKYKKKKSSFKRFLALTFKKKTENKLHVDVNVSSSRSSSESSYHGPSRILEVDRRSLSNSPQLKSRTGKLRASESPSSLIFYRDGKRKGVPFSRTVSRVESFEDRSRPPFLPLPLTKPRSISFPSADTSDYENIPAMNSDYENIQIPPRRPARAGAFTKLFEDQSRALSTANENDGYVDMSSFNAFESKQQSADQDAESAYTEPYKVCPISSAAPKEDLTSDEEQRSSEEEDSASRDPSVTHKVEGQSRALVIAQELLSSEKAYVEMLQHLNLDFHGAVMRALDDMDHEGRDTLAREELRQGLSELPAIHDLHQGILEELEERLSNWESQQKVADVFLAREQGFDHHATHILQFDRYLGLLSENCLHSPRLAAAVREFEQSVQGGSQTAKHRLLRVVQRLFQYQVLLTDYLNNLCPDSAEYDNTQGALSLISKVTDRANDSMEQGENLQKLVHIEHSVRGQGDLLQPGREFLKEGTLMKVTGKNRRPRHLFLMNDVLLYTYPQKDGKYRLKNTLAVANMKVSRPVMEKVPYALKIETSESCLMLSASSCAERDEWYGCLSRALPEDYKAQALAAFHHSVEIRERLGVSLGERPPTLVPVTHVMMCMNCGCDFSLTLRRHHCHACGKIVCRNCSRNKYPLKYLKDRMAKVCDGCFGELKKRGRAVPGLMRVTERPVSMSFPLSSPRFSGSAFSSVFQSINPSTFKKQKKVPSALTEDKVALESMPLLGFTIAPEKEEGSSEVGPIFHLYHKKTLFYSFKAEDTNSAQRWIEAMEDASVL

Target ORF information:

RefSeq Version	XM_011533423.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FYVE, RhoGEF and PH domain containing 5 (FGD5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533423.1

ORF Insert Sequence:

ATGTTCAGGG GTCCGAAGCC CCCCATTGCC CCCAAGCCCA GGCTGACTGC CCCAAACGAG 
TGGAGAGCCA GTGTGTACCT GAATGACAGC TTGAACAAAT GCAGCAACGG GCGGCTGCCC 
TGTGTAGACA GGGGGCTTGA TGAGGGGCCC CGGTCCATCC CAAAGTGCTC TGAGTCGGAG 
ACCGACGAGG ATTACATCGT GGTCCCCAGG GTTCCGCTGA GGGAGGATGA ACCCAAGGAC 
GAGGGCAGTG TGGGGAACAA AGCCCTGGTG TCTCCCGAGT CCTCTGCGGA AGAGGAAGAG 
GAGCGTGAAG AGGGAGGCGA GGCATGTGGC CTGGAGGGTA CAGGAGCTGG TGAGGATTCA 
GTGGCCCCTG CTGCTCCGGG TGCAGGAGCG CTGAGCAGGG AGGGTGAGGA AGGCACAGAC 
CTTGCTCTTG AGGATGAAGG GGAGGGCTGC GCTGATGAGC CAGGGACACT GGAGCAGGTG 
TCCAGAAGTG AGGAGGAAGA GAAGCTAGTG CAGCCACACA GGGAGTGCAG CCTGGAGGAC 
AGTGGGCCTT GGGCTGGAGA GGGGGTCTTC CAGAGCGACC TCCTCCTGCC TCACATCCAT 
GGAGAGGACC AGGAGCCCCC CGACACCCCC GGGGAGGCAG AGGAGGATGA TGAGGAAGGC 
TGTGCCAGCA CAGACCCAGC AGGGGCAGAT GAGGGTTCGG GTCCTGACAG GCCCACGGAG 
GACATGGGAC AGGATGCTGA GGACACCAGT GAGGAGCCCC CTGAGAAGGA GGAGCTGGCC 
GGGGTCCAGG AGGCAGAGAC AGCCACAGAC TGCCCTGAAG TTCTTGAGGA GGGATGTGAA 
GAGGCCACGG GTGTCACAGG TGGGGAACAG GTTGACCTCA GTGAACCACC TGACCACGAG 
AAGAAAACCA ACCAAGAAGT GGCAGCCGCC ACCCTGGAGG ACCATGCACA GGATGAGTCC 
GCCGAGGAGA GCTGCCAGAT TGTCCCTTTT GAGAATGACT GCATGGAGGA CTTCGTGACT 
TCCCTCACAG GAAGCCCCTA TGAGTTCTTC CCAACTGAGA GCACCTCTTT TTGCAGCGAG 
AGCTGTTCTC CTCTTTCTGA ATCAGCGAAA GGTTTAGAAT CAGAGCAGGC ACCAAAGCTG 
GGGCTGCGTG CGGAGGAGAA CCCCATGGTG GGGGCTTTGT GTGGCCAGTG TGGCTCCCTA 
CAGGGTGGAG CGGCCGAGGG TCCCGCAGCC CCTGATGTGG TGGTCGTGCT GGAGGAGGAG 
GCCTTGGATG ATGCACTGGC CAACCCCTAT GTGATGGGAG TGGGCCTGCC CGGTCAGGCG 
GCCCCTGGAG AAGGAGGGCA GGCTGCATCG GACGCCCTGG GTGGTTATGG CTCGAAAGAA 
GAATTGAACT GTGAGGCAGA GGGTGGCCTG GTTCCCGCGG ACAGGAAGAA CACCAGCACG 
AGGGTCCGGC CCCACTCTGG GAAGGTGGCC GGCTATGTCC CAGAAACCGT CCCTGAAGAA 
ACCGGACCTG AGGCGGGCTC GTCAGCCCCT GGCATTGGAG GTGCCGCAGA GGAGGTGGGA 
AAGACGCTTT TGTCATTGGA GGGGAAGCCC TTGGAAGCCA GCAGGGCCTT GCCAGCAAAG 
CCCAGGGCCT TTACTTTATA CCCTCGGTCG TTCTCCGTGG AAGGCCGAGA GATTCCAGTG 
TCCGTGTACC AGGAGCCTGA GGGGTCAGGG TTGGATGACC ACAGGATAAA GAGGAAAGAG 
GACAATCTCT CTCTGTCGTG TGTAATTGGC TCCTCTGGGA GTTTCTCCCA GAGAAACCAC 
CTTCCGTCCA GCGGCACCTC CACGCCTTCT TCCATGGTCG ACATCCCACC TCCTTTCGAC 
CTGGCCTGCA TCACCAAGAA GCCCATCACA AAGAGCTCTC CCTCACTCCT GATCGAGAGC 
GACTCCCCGG ACAAGTACAA GAAGAAGAAG TCATCCTTTA AGCGCTTCCT GGCACTGACG 
TTTAAGAAGA AGACGGAGAA CAAATTGCAT GTGGATGTGA ACGTGTCTTC CTCTAGGTCC 
TCTTCAGAGT CCAGCTACCA CGGGCCTTCC AGGATTCTGG AAGTTGACCG GAGAAGCCTC 
AGCAACTCCC CTCAGCTTAA GTCTCGGACT GGGAAGCTCC GGGCTTCTGA ATCCCCCTCC 
TCCCTCATCT TTTATAGAGA TGGCAAGAGG AAAGGTGTCC CCTTCAGCAG GACGGTGTCC 
AGAGTGGAGT CCTTTGAAGA CCGCTCCCGG CCGCCCTTCC TGCCCTTGCC ACTGACCAAG 
CCACGGTCCA TCTCCTTCCC CAGCGCTGAC ACTTCAGACT ATGAGAACAT TCCAGCCATG 
AACTCGGACT ATGAGAATAT CCAGATTCCA CCCCGGAGAC CTGCCAGGGC TGGCGCGTTC 
ACGAAGCTGT TTGAAGATCA GAGCAGAGCC CTGTCCACAG CAAACGAAAA TGATGGCTAC 
GTGGACATGA GCAGCTTCAA CGCCTTTGAG AGCAAACAGC AGAGTGCAGA CCAGGACGCA 
GAAAGCGCCT ACACAGAGCC CTACAAAGTC TGTCCCATCT CGTCGGCAGC CCCCAAAGAG 
GACCTTACGT CGGATGAAGA GCAGAGAAGC TCGGAGGAGG AGGACAGTGC TTCAAGAGAC 
CCCAGTGTCA CCCACAAGGT GGAAGGACAG TCCAGAGCCC TTGTCATCGC ACAGGAACTG 
CTATCTTCAG AGAAAGCATA CGTGGAGATG CTCCAGCACT TAAATCTGGA TTTCCATGGA 
GCTGTCATGA GGGCCTTGGA TGACATGGAC CATGAAGGCA GAGACACATT GGCCCGGGAG 
GAGCTGAGGC AGGGCCTGAG TGAACTCCCA GCCATCCACG ACCTTCATCA AGGCATCCTG 
GAGGAGCTGG AGGAAAGGCT GTCAAATTGG GAGAGCCAGC AGAAGGTAGC TGACGTCTTC 
CTGGCCCGGG AGCAGGGGTT TGATCACCAC GCCACTCACA TCCTGCAGTT CGACAGGTAC 
CTAGGTCTGC TCAGTGAGAA TTGCCTCCAC TCTCCCCGGC TGGCAGCTGC TGTCCGTGAA 
TTTGAGCAGA GTGTACAAGG AGGCAGCCAG ACTGCGAAGC ATCGGCTGCT GCGGGTGGTT 
CAACGCCTCT TCCAGTACCA AGTGCTCCTC ACAGACTATT TAAACAACCT TTGTCCGGAC 
TCCGCCGAGT ACGACAACAC ACAGGGTGCA CTGAGCCTCA TCTCCAAAGT CACAGACCGT 
GCCAACGACA GCATGGAGCA AGGGGAAAAC CTGCAGAAGC TGGTCCACAT TGAGCACAGC 
GTCCGGGGCC AAGGGGATCT CCTCCAGCCA GGAAGGGAGT TTCTGAAGGA AGGGACGCTG 
ATGAAAGTAA CAGGGAAAAA CAGACGGCCC CGGCACCTAT TTCTGATGAA CGATGTGCTC 
CTGTACACCT ATCCCCAGAA GGATGGGAAG TACCGGCTGA AGAACACATT GGCTGTGGCC 
AACATGAAGG TCAGCCGCCC TGTGATGGAG AAAGTGCCCT ACGCTCTAAA GATTGAGACT 
TCCGAGTCCT GCCTGATGCT GTCTGCGAGC TCCTGTGCAG AGAGGGACGA GTGGTATGGC 
TGTCTGAGCA GAGCCCTCCC TGAGGACTAC AAGGCCCAGG CGCTGGCTGC ATTCCACCAT 
AGCGTGGAGA TACGAGAGAG GCTGGGGGTT AGCCTTGGGG AGAGGCCCCC CACCCTGGTG 
CCTGTCACAC ACGTCATGAT GTGCATGAAC TGCGGCTGCG ACTTCTCCCT CACCCTGCGG 
CGTCATCACT GTCACGCCTG TGGCAAGATC GTGTGCCGGA ACTGTTCGCG GAACAAGTAC 
CCGCTGAAGT ACCTGAAGGA CAGGATGGCC AAGGTCTGCG ACGGCTGCTT CGGGGAGCTG 
AAGAAGCGGG GCAGGGCTGT CCCGGGCCTG ATGAGAGTTA CAGAGCGGCC TGTGAGCATG 
AGCTTCCCGC TGTCTTCACC CCGCTTCTCG GGCAGTGCCT TTTCATCCGT CTTCCAGAGC 
ATTAACCCCT CGACCTTCAA GAAGCAGAAG AAAGTCCCTT CAGCCCTGAC AGAGGACAAA 
GTGGCCTTGG AGAGTATGCC TCTGCTAGGC TTCACCATTG CTCCAGAAAA GGAAGAGGGC 
AGCAGTGAAG TAGGACCTAT TTTTCACCTT TACCACAAGA AAACCCTATT TTATAGCTTC 
AAAGCAGAAG ATACCAATTC AGCTCAGAGG TGGATCGAGG CCATGGAAGA TGCGAGTGTG 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FGD5 Regulates VEGF Receptor-2 Coupling to PI3 Kinase and Receptor Recycling.
Arteriosclerosis, thrombosis, and vascular biology37(12)2301-2310(2017 Dec)
Farhan MA,Azad AK,Touret N,Murray AG

FGD5 sustains vascular endothelial growth factor A (VEGFA) signaling through inhibition of proteasome-mediated VEGF receptor 2 degradation.
Cellular signalling40125-132(2017 Dec)
Heldin J,O'Callaghan P,Hern?ndez Vera R,Fuchs PF,Gerwins P,Kreuger J

FGD5 amplification in breast cancer patients is associated with tumour proliferation and a poorer prognosis.
Breast cancer research and treatment162(2)243-253(2017 04)
Valla M,Engstr?m MJ,Ytterhus B,Hansen ?K,Akslen LA,Vatten LJ,Opdahl S,Bofin AM

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FGD5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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