CARMIL2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CARMIL2
Entrez Gene ID	146206
Full Name	capping protein regulator and myosin 1 linker 2
Synonyms	CARMIL2b,LRRC16C,RLTPR
General protein information	Preferred Names capping protein regulator and myosin 1 linker 2 Names capping protein, Arp2/3 and myosin-I linker protein 2 F-actin-uncapping protein RLTPR RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing RGD, leucine-rich repeat, tropomodulin and proline-rich containing protein leucine rich repeat containing 16C leucine-rich repeat-containing protein 16C
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q22.1
Summary	This gene encodes a member of the CARMIL (capping protein, Arp2/3, myosin-I linker) family of proteins. The encoded protein interacts with and negatively regulates the heterodimeric capping protein and promotes cell migration. Reduced expression of this gene has been observed in human psoriasis patients. Mutations in this gene cause a human immunodeficiency syndrome characterized by smooth muscle tumors and impaired T-cell function. [provided by RefSeq, May 2017].
Disorder MIM:	610859

mRNA and Protein(s)

mRNA	Protein	Name
XM_017022953.1	XP_016878442.1	capping protein, Arp2/3 and myosin-I linker protein 2 isoform X2
XM_011522874.1	XP_011521176.1	capping protein, Arp2/3 and myosin-I linker protein 2 isoform X3
NM_001013838.3	NP_001013860.1	capping protein, Arp2/3 and myosin-I linker protein 2 isoform 1
XM_011522875.2	XP_011521177.2	capping protein, Arp2/3 and myosin-I linker protein 2 isoform X1
NM_001317026.3	NP_001303955.1	capping protein, Arp2/3 and myosin-I linker protein 2 isoform 2
NM_001317026.2	NP_001303955.1	capping protein, Arp2/3 and myosin-I linker protein 2 isoform 2
NM_001013838.2	NP_001013860.1	capping protein, Arp2/3 and myosin-I linker protein 2 isoform 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	si:dkey-6e12.4	XP_696152.5
Homo sapiens (human)	RLTPR	NP_001013860.1
	LOC699104	XP_002802556.1
Bos taurus (cattle)	RLTPR	XP_003587270.2
Xenopus tropicalis (tropical clawed frog)	LOC100491722	XP_002935414.2
Mus musculus (house mouse)	Rltpr	NP_001028492.1
Rattus norvegicus (Norway rat)	Rltpr	XP_006255611.1
Gallus gallus (chicken)	RLTPR	XP_414033.4

Gene Ontology
Bibliography

Related articles in PubMed

Genomic analysis of 220 CTCLs identifies a novel recurrent gain-of-function alteration in RLTPR (p.Q575E).
Park J, Yang J, Wenzel AT, Ramachandran A, Lee WJ, Daniels JC, Kim J, Martinez-Escala E, Amankulor N, Pro B, Guitart J, Mendillo ML, Savas JN, Boggon TJ, Choi J
Blood130(12)1430-1440(2017 09)

"Exome sequencing identifies a novel homozygous variant in NDRG4 in a family with infantile myofibromatosis (Linhares et?al., 2014)" turns out to be EBV+ leiomyomatosis caused by CARMIL2 mutations.
Linhares ND, Freire MC, Cardenas RG, Pena HB, Bahia M, Pena SD
European journal of medical genetics(2017 Feb)

A human immunodeficiency syndrome caused by mutations in CARMIL2.
Schober T, Magg T, Laschinger M, Rohlfs M, Linhares ND, Puchalka J, Weisser T, Fehlner K, Mautner J, Walz C, Hussein K, Jaeger G, Kammer B, Schmid I, Bahia M, Pena SD, Behrends U, Belohradsky BH, Klein C, Hauck F
Nature communications814209(2017 Jan)

A potential founder variant in
Sorte HS, Osnes LT, Fevang B, Aukrust P, Erichsen HC, Backe PH, Abrahamsen TG, Kittang OB, ?verland T, Jhangiani SN, Muzny DM, Vigeland MD, Samarakoon P, Gambin T, Akdemir ZH, Gibbs RA, R?dningen OK, Lyle R, Lupski JR, Stray-Pedersen A
Molecular genetics & genomic medicine4(6)604-616(2016 Nov)

Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations.
Wang Y, Ma CS, Ling Y, Bousfiha A, Camcioglu Y, Jacquot S, Payne K, Crestani E, Roncagalli R, Belkadi A, Kerner G, Lorenzo L, Deswarte C, Chrabieh M, Patin E, Vincent QB, M?ller-Fleckenstein I, Fleckenstein B, Ailal F, Quintana-Murci L, Fraitag S, Alyanakian MA, Leruez-Ville M, Picard C, Puel A, Bustamante J, Boisson-Dupuis S, Malissen M, Malissen B, Abel L, Hovnanian A, Notarangelo LD, Jouanguy E, Tangye SG, B?ziat V, Casanova JL
The Journal of experimental medicine213(11)2413-2435(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RLTPR (p.Q575E) increases binding of RLTPR to downstream components of the NF-kappaB signaling pathway, selectively upregulates the NF-kappaB pathway in activated T cells.
Title: Genomic analysis of 220 CTCLs identifies a novel recurrent gain-of-function alteration in RLTPR (p.Q575E).

Human RLTPR deficiency is a Combined immunodeficiency affecting at least the CD28-responsive pathway in T cells and the BCR-responsive pathway in B cells.
Title: Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations.

the scaffolding role of RLTPR predominates during CD28 co-stimulation and underpins the similar function of RLTPR in human and mouse T cells.
Title: The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells.

Data suggest that CARMIL2 displays two localization patterns in cells; one coincides with vimentin intermediate filaments, and the other coincides with cell membrane at leading edge of migrating cells.
Title: Cell Migration and Invadopodia Formation Require a Membrane-binding Domain of CARMIL2.

On the basis of these results, we propose a model in which dynamic vimentin filaments target CARMIL2 to critical membrane-associated locations, where CARMIL2 regulates CP, and thus actin assembly, to create cell protrusions
Title: CARMIL2 is a novel molecular connection between vimentin and actin essential for cell migration and invadopodia formation.

The following CARMIL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CARMIL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04135	NM_001013838.3 Latest version!	Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu49273	NM_001317026.3 Latest version!	Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu76205	XM_011522874.1 Latest version!	Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu85864	XM_011522875.2 Latest version!	Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu85865	XM_017022953.1 Latest version!	Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
View Old Accession Versions >>
OHu49273	NM_001317026.2	Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu04135	NM_001013838.2	Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CARMIL2 ( NM_001013838.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001013860.1 Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04135
Clone ID Related Accession (Same CDS sequence)	NM_001013838.3 , NM_001013838.2
Accession Version	NM_001013838.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	capping protein, Arp2/3 and myosin-I linker protein 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB113647.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001013838.2. Transcript Variant: This variant (1) retains an intron in the 5' coding region and contains an alternate in-frame exon in the 3' coding region compared to variant 2. The encoded isoform (1, also known as CARMIL2a) has the same N- and C-termini but is longer compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB113647.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000334583.11/ ENSP00000334958.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCAGA CCCCCGACGG CATCTCCTGT GAGCTCCGAG GCGAGATCAC CAGGTTCCTG 
TGGCCCAAAG AGGTGGAGCT GCTGCTGAAA ACCTGGCTAC CCGGGGAGGG TGCTGTGCAA 
AACCATGTCC TGGCACTGCT ACGATGGAGA GCCTACCTGC TGCACACCAC CTGCCTCCCG 
CTGAGGGTGG ACTGCACGTT CAGCTACCTG GAGGTCCAGG CCATGGCGCT GCAGGAGACA 
CCCCCTCAGG TCACCTTTGA GCTGGAGTCC CTGCGTGAGC TGGTCCTGGA GTTTCCTGGT 
GTGGCCGCCC TGGAACAGCT GGCCCAGCAC GTGGCTGCAG CCATCAAGAA GGTCTTCCCT 
CGCTCGACCC TTGGGAAGCT ATTCCGGAGG CCCACACCAG CCTCCATGCT GGCTCGGCTG 
GAGAGAAGCA GCCCCTCGGA GTCCACTGAC CCCTGCAGCC CCTGTGGTGG CTTCTTGGAG 
ACATACGAGG CTCTGTGTGA CTACAATGGC TTCCCTTTCC GAGAGGAGAT TCAGTGGGAC 
GTGGACACCA TTTACCATCG CCAGGGCTGC CGCCATTTCA GCCTGGGAGA CTTCAGCCAC 
CTCGGCAGTC GGGACCTGGC CTTGAGTGTG GCTGCCCTGT CCTACAACCT GTGGTTCCGG 
TGCCTCTCCT GTGTGGACAT GAAGCTGAGC CTTGAGGTCT CAGAACAGAT TCTGCACATG 
ATGAGTCAGT CATCACACCT GGAGGAGCTG GTGCTGGAGA CCTGCAGCCT GAGGGGAGAC 
TTTGTCCGAC GACTGGCCCA GGCGCTGGCG GGACACTCAA GCTCTGGGCT GCGGGAGCTC 
AGCCTCGCGG GGAACCTGCT GGATGACCGA GGCATGACTG CACTCAGCAG ACACCTCGAG 
CGTTGTCCAG GAGCCCTGAG GAGACTCAGC CTGGCCCAGA CAGGGTTGAC ACCGCGAGGA 
ATGAGGGCTC TGGGCCGGGC ACTGGCCACC AATGCCGCCT TCGACTCCAC CCTGACCCAC 
CTGGACCTTT CTGGGAATCC TGGGGCGCTG GGGGCCTCCG AGGACAGTGG GGGCCTCTAT 
AGCTTCCTGA GCCGTCCTAA CGTACTGTCG TTCCTGAATC TCGCAGGCAC CGACACTGCC 
CTGGACACTG TGAGGGGGTG CTCCGTGGGG GGATGGATGA CCGGCAGGGC GGACTGGAGG 
GCGGGACGGG GAGGGCTCGG TCCCCCCGCG GGTGTAGCCA ACAGCCTCCC CCCGCAGCTC 
TTCGCAGCGG TATCCCGAGG CTGCTGCACC AGCCTTACCC ACCTCGACGC TTCGAGGAAC 
GTCTTCTCCC GCACGAAGTC CCGCGCCGCG CCGGCCGCGC TGCAGCTCTT CCTCAGCCGC 
GCGCGGACGC TGCGGCACCT GGGCCTGGCG GGCTGCAAGC TGCCGCCCGA CGCGCTCAGA 
GCCCTTTTGG ATGGCCTCGC GCTCAACACG CACCTCCGCG ACCTGCACCT GGACCTCAGC 
GCTTGCGAGC TGCGCTCGGC CGGCGCCCAG GTGATACAAG ACTTAGTGTG CGACGCAGGC 
GCTGTGAGCT CCCTGGATCT GGCGGATAAC GGCTTCGGCT CAGACATGGT GACTCTGGTG 
CTGGCCATCG GGAGAAGCCG GTCCCTGAGA CATGTGGCGC TTGGAAGGAA CTTCAACGTC 
CGGTGCAAGG AGACCCTGGA CGACGTCCTG CACCGGATTG TCCAGCTCAT GCAGGACGAC 
GATTGTCCTC TTCAGTCTCT GTCGGTGGCT GAGTCTCGGC TGAAGCTGGG TGCCAGCGTC 
CTACTCCGGG CCCTAGCCAC CAATCCTAAC CTGACCGCGC TGGATATCAG CGGCAACGCC 
ATGGGGGACG CGGGCGCCAA GTTGCTGGCC AAGGCGCTGC GGGTCAACTC GAGGCTCCGG 
TCTGTGGTCT GGGACCGGAA CCACACATCT GCTTTGGGTC TGCTGGACGT GGCGCAGGCG 
CTGGAGCAGA ACCACAGCCT GAAGGCCATG CCTCTGCCAC TGAACGACGT GGCCCAGGCG 
CAGCGCAGCC GCCCGGAACT GACAGCACGT GCAGTCCATC AGATCCAAGC CTGTCTCTTG 
AGGAACAACC GCGCAGACCC TGCCTCTTCT GACCACACGA CCCGCCTTCA GCCACTTGGT 
CTGGTCTCAG ACCCCTCAGA GCAGGAAGTG AATGAATTGT GTCAGTCGGT GCAGGAGCAT 
GTGGAGCTGC TGGGCTGTGG GGCTGGGCCC CAGGGTGAAG CCGCTGTGCG CCAGGCCGAG 
GATGCCATCC AAAATGCCAA CTTCTCTCTC AGCATTCTCC CCATTCTATA TGAAGCTGGA 
AGCTCCCCAA GCCATCACTG GCAGCTTGGG CAGAAGCTGG AGGGCCTTCT GAGACAGGTG 
GGCGAGGTCT GCCGCCAGGA CATCCAGGAC TTCACTCAGG CCACACTGGA CACAGCAAGG 
AGCCTCTGCC CACAGATGCT GCAGGGATCC AGCTGGAGGG AGCAGCTAGA GGGGGTCCTG 
GCAGGCTCGA GGGGCCTCCC GGAGCTGCTC CCAGAGCAGC TGCTGCAAGA TGCCTTCACT 
AGGCTCAGGG ACATGCGGCT ATCAATCACG GGGACCTTGG CAGAGAGCAT TGTGGCTCAG 
GCTTTGGCAG GCCTGAGTGC AGCCCGGGAT CAGCTGGTGG AGAGTCTGGC TCAGCAGGCA 
ACAGTGACAA TGCCCCCTGC CCTACCAGCA CCGGATGGAG GTGAGCCCAG CCTCCTTGAG 
CCTGGGGAAT TGGAAGGTCT TTTCTTCCCC GAGGAGAAGG AAGAGGAGAA GGAGAAGGAT 
GACAGTCCTC CACAGAAATG GCCTGAGCTC AGCCACGGTC TTCACCTGGT CCCCTTCATT 
CACAGTGCTG CTGAGGAAGC GGAGCCGGAG CCCGAGCTGG CGGCTCCGGG AGAAGATGCA 
GAGCCGCAGG CGGGGCCGTC CGCGCGCGGC TCTCCGAGCC CTGCCGCCCC TGGGCCCCCG 
GCCGGCCCGC TGCCCCGCAT GGACCTGCCA CTGGCGGGGC AGCCCCTGCG CCATCCGACC 
CGGGCCCGGC CGCGGCCGCG CCGCCAGCAC CACCACCGCC CGCCGCCGGG GGGCCCCCAG 
GTACCCCCAG CCTTGCCGCA GGAAGGGAAT GGGCTCAGTG CCCGCGTGGA CGAGGGCGTG 
GAGGAATTCT TCTCCAAAAG GCTGATCCAG CAGGATCGCC TCTGGGCCCC CGAGGAGGAC 
CCGGCCACTG AGGGGGGCGC CACTCCTGTC CCCCGTACAC TGCGAAAGAA GCTGGGCACC 
CTCTTTGCCT TCAAGAAGCC TCGTTCAACG CGGGGTCCAC GGACTGATCT AGAGACCAGC 
CCTGGGGCAG CTCCCCGAAC CCGAAAAACT ACATTTGGCG ACCTACTGCG GCCGCCAACC 
CGTCCCAGCC GTGGTGAGGA GCTTGGTGGG GCTGAGGGGG ACACCAGCAG CCCTGACCCT 
GCCGGCAGGA GCCGACCTCG CTACACAAGA GATAGCAAGG CCTACTCGAT GATACTGCTG 
CCTGCCGAGG AGGAGGCAAC GCTGGGTGCC AGACCCGACA AGCGGCGGCC CCTGGAGCGG 
GGAGAAACAG AACTGGCTCC ATCCTTTGAA CAGCGGGTAC AAGTAATGCT GCAGAGGATA 
GGCGTCAGCC GAGGCAGCGG GGGTGCCGAA GGCAAGAGGA AGCAAAGCAA AGATGGCGAG 
ATCAAGAAAG CTGGCTCAGA TGGTGACATT ATGGACAGTT CCACGGAGGC CCCTCCCATC 
TCGATCAAGT CCCGCACCCA CTCTGTGTCT GCTGACCCTT CCTGCAGACC TGGCCCAGGG 
AGCCAGGGGC CTGAGTCTGC CACCTGGAAG ACACTGGGGC AGCAGTTGAA TGCGGAGCTC 
AGGAGCCGTG GTTGGGGCCA ACAGGATGGT CCAGGCCCTC CCTCCCCTGG TCAAAGCCCA 
AGTCCCTGCA GAACCAGCCC CTCCCCAGAC AGCCTGGGCC TCCCAGAGGA CCCTTGCTTG 
GGCCCCAGAA ATGAAGATGG CCAGCTGAGG CCGAGGCCTC TCTCGGCAGG GCGGCGAGCA 
GTGTCTGTGC ATGAGGACCA GCTCCAGGCC CCTGCTGAAC GGCCCCTGAG GCTGCAGCGC 
TCCCCCGTCC TCAAACGCAG GCCAAAACTC GAGGCACCTC CATCCCCAAG CCTAGGATCT 
GGCCTTGGAA CCGAGCCTCT GCCCCCACAG CCCACAGAGC CCTCCAGCCC TGAGCGGAGC 
CCACCCTCCC CAGCCACAGA CCAAAGAGGC GGCGGCCCCA ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013860.1
CDS	104..4411
Translation	MAQTPDGISCELRGEITRFLWPKEVELLLKTWLPGEGAVQNHVLALLRWRAYLLHTTCLPLRVDCTFSYLEVQAMALQETPPQVTFELESLRELVLEFPGVAALEQLAQHVAAAIKKVFPRSTLGKLFRRPTPASMLARLERSSPSESTDPCSPCGGFLETYEALCDYNGFPFREEIQWDVDTIYHRQGCRHFSLGDFSHLGSRDLALSVAALSYNLWFRCLSCVDMKLSLEVSEQILHMMSQSSHLEELVLETCSLRGDFVRRLAQALAGHSSSGLRELSLAGNLLDDRGMTALSRHLERCPGALRRLSLAQTGLTPRGMRALGRALATNAAFDSTLTHLDLSGNPGALGASEDSGGLYSFLSRPNVLSFLNLAGTDTALDTVRGCSVGGWMTGRADWRAGRGGLGPPAGVANSLPPQLFAAVSRGCCTSLTHLDASRNVFSRTKSRAAPAALQLFLSRARTLRHLGLAGCKLPPDALRALLDGLALNTHLRDLHLDLSACELRSAGAQVIQDLVCDAGAVSSLDLADNGFGSDMVTLVLAIGRSRSLRHVALGRNFNVRCKETLDDVLHRIVQLMQDDDCPLQSLSVAESRLKLGASVLLRALATNPNLTALDISGNAMGDAGAKLLAKALRVNSRLRSVVWDRNHTSALGLLDVAQALEQNHSLKAMPLPLNDVAQAQRSRPELTARAVHQIQACLLRNNRADPASSDHTTRLQPLGLVSDPSEQEVNELCQSVQEHVELLGCGAGPQGEAAVRQAEDAIQNANFSLSILPILYEAGSSPSHHWQLGQKLEGLLRQVGEVCRQDIQDFTQATLDTARSLCPQMLQGSSWREQLEGVLAGSRGLPELLPEQLLQDAFTRLRDMRLSITGTLAESIVAQALAGLSAARDQLVESLAQQATVTMPPALPAPDGGEPSLLEPGELEGLFFPEEKEEEKEKDDSPPQKWPELSHGLHLVPFIHSAAEEAEPEPELAAPGEDAEPQAGPSARGSPSPAAPGPPAGPLPRMDLPLAGQPLRHPTRARPRPRRQHHHRPPPGGPQVPPALPQEGNGLSARVDEGVEEFFSKRLIQQDRLWAPEEDPATEGGATPVPRTLRKKLGTLFAFKKPRSTRGPRTDLETSPGAAPRTRKTTFGDLLRPPTRPSRGEELGGAEGDTSSPDPAGRSRPRYTRDSKAYSMILLPAEEEATLGARPDKRRPLERGETELAPSFEQRVQVMLQRIGVSRGSGGAEGKRKQSKDGEIKKAGSDGDIMDSSTEAPPISIKSRTHSVSADPSCRPGPGSQGPESATWKTLGQQLNAELRSRGWGQQDGPGPPSPGQSPSPCRTSPSPDSLGLPEDPCLGPRNEDGQLRPRPLSAGRRAVSVHEDQLQAPAERPLRLQRSPVLKRRPKLEAPPSPSLGSGLGTEPLPPQPTEPSSPERSPPSPATDQRGGGPNP

Target ORF information:

RefSeq Version	NM_001013838.3
Organism	Homo sapiens(human)
Definition	Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013838.3

ORF Insert Sequence:

ATGGCCCAGA CCCCCGACGG CATCTCCTGT GAGCTCCGAG GCGAGATCAC CAGGTTCCTG 
TGGCCCAAAG AGGTGGAGCT GCTGCTGAAA ACCTGGCTAC CCGGGGAGGG TGCTGTGCAA 
AACCATGTCC TGGCACTGCT ACGATGGAGA GCCTACCTGC TGCACACCAC CTGCCTCCCG 
CTGAGGGTGG ACTGCACGTT CAGCTACCTG GAGGTCCAGG CCATGGCGCT GCAGGAGACA 
CCCCCTCAGG TCACCTTTGA GCTGGAGTCC CTGCGTGAGC TGGTCCTGGA GTTTCCTGGT 
GTGGCCGCCC TGGAACAGCT GGCCCAGCAC GTGGCTGCAG CCATCAAGAA GGTCTTCCCT 
CGCTCGACCC TTGGGAAGCT ATTCCGGAGG CCCACACCAG CCTCCATGCT GGCTCGGCTG 
GAGAGAAGCA GCCCCTCGGA GTCCACTGAC CCCTGCAGCC CCTGTGGTGG CTTCTTGGAG 
ACATACGAGG CTCTGTGTGA CTACAATGGC TTCCCTTTCC GAGAGGAGAT TCAGTGGGAC 
GTGGACACCA TTTACCATCG CCAGGGCTGC CGCCATTTCA GCCTGGGAGA CTTCAGCCAC 
CTCGGCAGTC GGGACCTGGC CTTGAGTGTG GCTGCCCTGT CCTACAACCT GTGGTTCCGG 
TGCCTCTCCT GTGTGGACAT GAAGCTGAGC CTTGAGGTCT CAGAACAGAT TCTGCACATG 
ATGAGTCAGT CATCACACCT GGAGGAGCTG GTGCTGGAGA CCTGCAGCCT GAGGGGAGAC 
TTTGTCCGAC GACTGGCCCA GGCGCTGGCG GGACACTCAA GCTCTGGGCT GCGGGAGCTC 
AGCCTCGCGG GGAACCTGCT GGATGACCGA GGCATGACTG CACTCAGCAG ACACCTCGAG 
CGTTGTCCAG GAGCCCTGAG GAGACTCAGC CTGGCCCAGA CAGGGTTGAC ACCGCGAGGA 
ATGAGGGCTC TGGGCCGGGC ACTGGCCACC AATGCCGCCT TCGACTCCAC CCTGACCCAC 
CTGGACCTTT CTGGGAATCC TGGGGCGCTG GGGGCCTCCG AGGACAGTGG GGGCCTCTAT 
AGCTTCCTGA GCCGTCCTAA CGTACTGTCG TTCCTGAATC TCGCAGGCAC CGACACTGCC 
CTGGACACTG TGAGGGGGTG CTCCGTGGGG GGATGGATGA CCGGCAGGGC GGACTGGAGG 
GCGGGACGGG GAGGGCTCGG TCCCCCCGCG GGTGTAGCCA ACAGCCTCCC CCCGCAGCTC 
TTCGCAGCGG TATCCCGAGG CTGCTGCACC AGCCTTACCC ACCTCGACGC TTCGAGGAAC 
GTCTTCTCCC GCACGAAGTC CCGCGCCGCG CCGGCCGCGC TGCAGCTCTT CCTCAGCCGC 
GCGCGGACGC TGCGGCACCT GGGCCTGGCG GGCTGCAAGC TGCCGCCCGA CGCGCTCAGA 
GCCCTTTTGG ATGGCCTCGC GCTCAACACG CACCTCCGCG ACCTGCACCT GGACCTCAGC 
GCTTGCGAGC TGCGCTCGGC CGGCGCCCAG GTGATACAAG ACTTAGTGTG CGACGCAGGC 
GCTGTGAGCT CCCTGGATCT GGCGGATAAC GGCTTCGGCT CAGACATGGT GACTCTGGTG 
CTGGCCATCG GGAGAAGCCG GTCCCTGAGA CATGTGGCGC TTGGAAGGAA CTTCAACGTC 
CGGTGCAAGG AGACCCTGGA CGACGTCCTG CACCGGATTG TCCAGCTCAT GCAGGACGAC 
GATTGTCCTC TTCAGTCTCT GTCGGTGGCT GAGTCTCGGC TGAAGCTGGG TGCCAGCGTC 
CTACTCCGGG CCCTAGCCAC CAATCCTAAC CTGACCGCGC TGGATATCAG CGGCAACGCC 
ATGGGGGACG CGGGCGCCAA GTTGCTGGCC AAGGCGCTGC GGGTCAACTC GAGGCTCCGG 
TCTGTGGTCT GGGACCGGAA CCACACATCT GCTTTGGGTC TGCTGGACGT GGCGCAGGCG 
CTGGAGCAGA ACCACAGCCT GAAGGCCATG CCTCTGCCAC TGAACGACGT GGCCCAGGCG 
CAGCGCAGCC GCCCGGAACT GACAGCACGT GCAGTCCATC AGATCCAAGC CTGTCTCTTG 
AGGAACAACC GCGCAGACCC TGCCTCTTCT GACCACACGA CCCGCCTTCA GCCACTTGGT 
CTGGTCTCAG ACCCCTCAGA GCAGGAAGTG AATGAATTGT GTCAGTCGGT GCAGGAGCAT 
GTGGAGCTGC TGGGCTGTGG GGCTGGGCCC CAGGGTGAAG CCGCTGTGCG CCAGGCCGAG 
GATGCCATCC AAAATGCCAA CTTCTCTCTC AGCATTCTCC CCATTCTATA TGAAGCTGGA 
AGCTCCCCAA GCCATCACTG GCAGCTTGGG CAGAAGCTGG AGGGCCTTCT GAGACAGGTG 
GGCGAGGTCT GCCGCCAGGA CATCCAGGAC TTCACTCAGG CCACACTGGA CACAGCAAGG 
AGCCTCTGCC CACAGATGCT GCAGGGATCC AGCTGGAGGG AGCAGCTAGA GGGGGTCCTG 
GCAGGCTCGA GGGGCCTCCC GGAGCTGCTC CCAGAGCAGC TGCTGCAAGA TGCCTTCACT 
AGGCTCAGGG ACATGCGGCT ATCAATCACG GGGACCTTGG CAGAGAGCAT TGTGGCTCAG 
GCTTTGGCAG GCCTGAGTGC AGCCCGGGAT CAGCTGGTGG AGAGTCTGGC TCAGCAGGCA 
ACAGTGACAA TGCCCCCTGC CCTACCAGCA CCGGATGGAG GTGAGCCCAG CCTCCTTGAG 
CCTGGGGAAT TGGAAGGTCT TTTCTTCCCC GAGGAGAAGG AAGAGGAGAA GGAGAAGGAT 
GACAGTCCTC CACAGAAATG GCCTGAGCTC AGCCACGGTC TTCACCTGGT CCCCTTCATT 
CACAGTGCTG CTGAGGAAGC GGAGCCGGAG CCCGAGCTGG CGGCTCCGGG AGAAGATGCA 
GAGCCGCAGG CGGGGCCGTC CGCGCGCGGC TCTCCGAGCC CTGCCGCCCC TGGGCCCCCG 
GCCGGCCCGC TGCCCCGCAT GGACCTGCCA CTGGCGGGGC AGCCCCTGCG CCATCCGACC 
CGGGCCCGGC CGCGGCCGCG CCGCCAGCAC CACCACCGCC CGCCGCCGGG GGGCCCCCAG 
GTACCCCCAG CCTTGCCGCA GGAAGGGAAT GGGCTCAGTG CCCGCGTGGA CGAGGGCGTG 
GAGGAATTCT TCTCCAAAAG GCTGATCCAG CAGGATCGCC TCTGGGCCCC CGAGGAGGAC 
CCGGCCACTG AGGGGGGCGC CACTCCTGTC CCCCGTACAC TGCGAAAGAA GCTGGGCACC 
CTCTTTGCCT TCAAGAAGCC TCGTTCAACG CGGGGTCCAC GGACTGATCT AGAGACCAGC 
CCTGGGGCAG CTCCCCGAAC CCGAAAAACT ACATTTGGCG ACCTACTGCG GCCGCCAACC 
CGTCCCAGCC GTGGTGAGGA GCTTGGTGGG GCTGAGGGGG ACACCAGCAG CCCTGACCCT 
GCCGGCAGGA GCCGACCTCG CTACACAAGA GATAGCAAGG CCTACTCGAT GATACTGCTG 
CCTGCCGAGG AGGAGGCAAC GCTGGGTGCC AGACCCGACA AGCGGCGGCC CCTGGAGCGG 
GGAGAAACAG AACTGGCTCC ATCCTTTGAA CAGCGGGTAC AAGTAATGCT GCAGAGGATA 
GGCGTCAGCC GAGGCAGCGG GGGTGCCGAA GGCAAGAGGA AGCAAAGCAA AGATGGCGAG 
ATCAAGAAAG CTGGCTCAGA TGGTGACATT ATGGACAGTT CCACGGAGGC CCCTCCCATC 
TCGATCAAGT CCCGCACCCA CTCTGTGTCT GCTGACCCTT CCTGCAGACC TGGCCCAGGG 
AGCCAGGGGC CTGAGTCTGC CACCTGGAAG ACACTGGGGC AGCAGTTGAA TGCGGAGCTC 
AGGAGCCGTG GTTGGGGCCA ACAGGATGGT CCAGGCCCTC CCTCCCCTGG TCAAAGCCCA 
AGTCCCTGCA GAACCAGCCC CTCCCCAGAC AGCCTGGGCC TCCCAGAGGA CCCTTGCTTG 
GGCCCCAGAA ATGAAGATGG CCAGCTGAGG CCGAGGCCTC TCTCGGCAGG GCGGCGAGCA 
GTGTCTGTGC ATGAGGACCA GCTCCAGGCC CCTGCTGAAC GGCCCCTGAG GCTGCAGCGC 
TCCCCCGTCC TCAAACGCAG GCCAAAACTC GAGGCACCTC CATCCCCAAG CCTAGGATCT 
GGCCTTGGAA CCGAGCCTCT GCCCCCACAG CCCACAGAGC CCTCCAGCCC TGAGCGGAGC 
CCACCCTCCC CAGCCACAGA CCAAAGAGGC GGCGGCCCCA ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CARMIL2 ( NM_001013838.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001013860.1 Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04135
Clone ID Related Accession (Same CDS sequence)	NM_001013838.3 , NM_001013838.2
Accession Version	NM_001013838.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-03
Organism	Homo sapiens(human)
Product	capping protein, Arp2/3 and myosin-I linker protein 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009095.7 and AB113647.1. This sequence is a reference standard in the RefSeqGene project. On May 4, 2017 this sequence version replaced NM_001013838.1. Transcript Variant: This variant (1) retains an intron in the 5' coding region and contains an alternate in-frame exon in the 3' coding region compared to variant 2. The encoded isoform (1, also known as CARMIL2a) has the same N- and C-termini but is longer compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB113647.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAGA CCCCCGACGG CATCTCCTGT GAGCTCCGAG GCGAGATCAC CAGGTTCCTG 
TGGCCCAAAG AGGTGGAGCT GCTGCTGAAA ACCTGGCTAC CCGGGGAGGG TGCTGTGCAA 
AACCATGTCC TGGCACTGCT ACGATGGAGA GCCTACCTGC TGCACACCAC CTGCCTCCCG 
CTGAGGGTGG ACTGCACGTT CAGCTACCTG GAGGTCCAGG CCATGGCGCT GCAGGAGACA 
CCCCCTCAGG TCACCTTTGA GCTGGAGTCC CTGCGTGAGC TGGTCCTGGA GTTTCCTGGT 
GTGGCCGCCC TGGAACAGCT GGCCCAGCAC GTGGCTGCAG CCATCAAGAA GGTCTTCCCT 
CGCTCGACCC TTGGGAAGCT ATTCCGGAGG CCCACACCAG CCTCCATGCT GGCTCGGCTG 
GAGAGAAGCA GCCCCTCGGA GTCCACTGAC CCCTGCAGCC CCTGTGGTGG CTTCTTGGAG 
ACATACGAGG CTCTGTGTGA CTACAATGGC TTCCCTTTCC GAGAGGAGAT TCAGTGGGAC 
GTGGACACCA TTTACCATCG CCAGGGCTGC CGCCATTTCA GCCTGGGAGA CTTCAGCCAC 
CTCGGCAGTC GGGACCTGGC CTTGAGTGTG GCTGCCCTGT CCTACAACCT GTGGTTCCGG 
TGCCTCTCCT GTGTGGACAT GAAGCTGAGC CTTGAGGTCT CAGAACAGAT TCTGCACATG 
ATGAGTCAGT CATCACACCT GGAGGAGCTG GTGCTGGAGA CCTGCAGCCT GAGGGGAGAC 
TTTGTCCGAC GACTGGCCCA GGCGCTGGCG GGACACTCAA GCTCTGGGCT GCGGGAGCTC 
AGCCTCGCGG GGAACCTGCT GGATGACCGA GGCATGACTG CACTCAGCAG ACACCTCGAG 
CGTTGTCCAG GAGCCCTGAG GAGACTCAGC CTGGCCCAGA CAGGGTTGAC ACCGCGAGGA 
ATGAGGGCTC TGGGCCGGGC ACTGGCCACC AATGCCGCCT TCGACTCCAC CCTGACCCAC 
CTGGACCTTT CTGGGAATCC TGGGGCGCTG GGGGCCTCCG AGGACAGTGG GGGCCTCTAT 
AGCTTCCTGA GCCGTCCTAA CGTACTGTCG TTCCTGAATC TCGCAGGCAC CGACACTGCC 
CTGGACACTG TGAGGGGGTG CTCCGTGGGG GGATGGATGA CCGGCAGGGC GGACTGGAGG 
GCGGGACGGG GAGGGCTCGG TCCCCCCGCG GGTGTAGCCA ACAGCCTCCC CCCGCAGCTC 
TTCGCAGCGG TATCCCGAGG CTGCTGCACC AGCCTTACCC ACCTCGACGC TTCGAGGAAC 
GTCTTCTCCC GCACGAAGTC CCGCGCCGCG CCGGCCGCGC TGCAGCTCTT CCTCAGCCGC 
GCGCGGACGC TGCGGCACCT GGGCCTGGCG GGCTGCAAGC TGCCGCCCGA CGCGCTCAGA 
GCCCTTTTGG ATGGCCTCGC GCTCAACACG CACCTCCGCG ACCTGCACCT GGACCTCAGC 
GCTTGCGAGC TGCGCTCGGC CGGCGCCCAG GTGATACAAG ACTTAGTGTG CGACGCAGGC 
GCTGTGAGCT CCCTGGATCT GGCGGATAAC GGCTTCGGCT CAGACATGGT GACTCTGGTG 
CTGGCCATCG GGAGAAGCCG GTCCCTGAGA CATGTGGCGC TTGGAAGGAA CTTCAACGTC 
CGGTGCAAGG AGACCCTGGA CGACGTCCTG CACCGGATTG TCCAGCTCAT GCAGGACGAC 
GATTGTCCTC TTCAGTCTCT GTCGGTGGCT GAGTCTCGGC TGAAGCTGGG TGCCAGCGTC 
CTACTCCGGG CCCTAGCCAC CAATCCTAAC CTGACCGCGC TGGATATCAG CGGCAACGCC 
ATGGGGGACG CGGGCGCCAA GTTGCTGGCC AAGGCGCTGC GGGTCAACTC GAGGCTCCGG 
TCTGTGGTCT GGGACCGGAA CCACACATCT GCTTTGGGTC TGCTGGACGT GGCGCAGGCG 
CTGGAGCAGA ACCACAGCCT GAAGGCCATG CCTCTGCCAC TGAACGACGT GGCCCAGGCG 
CAGCGCAGCC GCCCGGAACT GACAGCACGT GCAGTCCATC AGATCCAAGC CTGTCTCTTG 
AGGAACAACC GCGCAGACCC TGCCTCTTCT GACCACACGA CCCGCCTTCA GCCACTTGGT 
CTGGTCTCAG ACCCCTCAGA GCAGGAAGTG AATGAATTGT GTCAGTCGGT GCAGGAGCAT 
GTGGAGCTGC TGGGCTGTGG GGCTGGGCCC CAGGGTGAAG CCGCTGTGCG CCAGGCCGAG 
GATGCCATCC AAAATGCCAA CTTCTCTCTC AGCATTCTCC CCATTCTATA TGAAGCTGGA 
AGCTCCCCAA GCCATCACTG GCAGCTTGGG CAGAAGCTGG AGGGCCTTCT GAGACAGGTG 
GGCGAGGTCT GCCGCCAGGA CATCCAGGAC TTCACTCAGG CCACACTGGA CACAGCAAGG 
AGCCTCTGCC CACAGATGCT GCAGGGATCC AGCTGGAGGG AGCAGCTAGA GGGGGTCCTG 
GCAGGCTCGA GGGGCCTCCC GGAGCTGCTC CCAGAGCAGC TGCTGCAAGA TGCCTTCACT 
AGGCTCAGGG ACATGCGGCT ATCAATCACG GGGACCTTGG CAGAGAGCAT TGTGGCTCAG 
GCTTTGGCAG GCCTGAGTGC AGCCCGGGAT CAGCTGGTGG AGAGTCTGGC TCAGCAGGCA 
ACAGTGACAA TGCCCCCTGC CCTACCAGCA CCGGATGGAG GTGAGCCCAG CCTCCTTGAG 
CCTGGGGAAT TGGAAGGTCT TTTCTTCCCC GAGGAGAAGG AAGAGGAGAA GGAGAAGGAT 
GACAGTCCTC CACAGAAATG GCCTGAGCTC AGCCACGGTC TTCACCTGGT CCCCTTCATT 
CACAGTGCTG CTGAGGAAGC GGAGCCGGAG CCCGAGCTGG CGGCTCCGGG AGAAGATGCA 
GAGCCGCAGG CGGGGCCGTC CGCGCGCGGC TCTCCGAGCC CTGCCGCCCC TGGGCCCCCG 
GCCGGCCCGC TGCCCCGCAT GGACCTGCCA CTGGCGGGGC AGCCCCTGCG CCATCCGACC 
CGGGCCCGGC CGCGGCCGCG CCGCCAGCAC CACCACCGCC CGCCGCCGGG GGGCCCCCAG 
GTACCCCCAG CCTTGCCGCA GGAAGGGAAT GGGCTCAGTG CCCGCGTGGA CGAGGGCGTG 
GAGGAATTCT TCTCCAAAAG GCTGATCCAG CAGGATCGCC TCTGGGCCCC CGAGGAGGAC 
CCGGCCACTG AGGGGGGCGC CACTCCTGTC CCCCGTACAC TGCGAAAGAA GCTGGGCACC 
CTCTTTGCCT TCAAGAAGCC TCGTTCAACG CGGGGTCCAC GGACTGATCT AGAGACCAGC 
CCTGGGGCAG CTCCCCGAAC CCGAAAAACT ACATTTGGCG ACCTACTGCG GCCGCCAACC 
CGTCCCAGCC GTGGTGAGGA GCTTGGTGGG GCTGAGGGGG ACACCAGCAG CCCTGACCCT 
GCCGGCAGGA GCCGACCTCG CTACACAAGA GATAGCAAGG CCTACTCGAT GATACTGCTG 
CCTGCCGAGG AGGAGGCAAC GCTGGGTGCC AGACCCGACA AGCGGCGGCC CCTGGAGCGG 
GGAGAAACAG AACTGGCTCC ATCCTTTGAA CAGCGGGTAC AAGTAATGCT GCAGAGGATA 
GGCGTCAGCC GAGGCAGCGG GGGTGCCGAA GGCAAGAGGA AGCAAAGCAA AGATGGCGAG 
ATCAAGAAAG CTGGCTCAGA TGGTGACATT ATGGACAGTT CCACGGAGGC CCCTCCCATC 
TCGATCAAGT CCCGCACCCA CTCTGTGTCT GCTGACCCTT CCTGCAGACC TGGCCCAGGG 
AGCCAGGGGC CTGAGTCTGC CACCTGGAAG ACACTGGGGC AGCAGTTGAA TGCGGAGCTC 
AGGAGCCGTG GTTGGGGCCA ACAGGATGGT CCAGGCCCTC CCTCCCCTGG TCAAAGCCCA 
AGTCCCTGCA GAACCAGCCC CTCCCCAGAC AGCCTGGGCC TCCCAGAGGA CCCTTGCTTG 
GGCCCCAGAA ATGAAGATGG CCAGCTGAGG CCGAGGCCTC TCTCGGCAGG GCGGCGAGCA 
GTGTCTGTGC ATGAGGACCA GCTCCAGGCC CCTGCTGAAC GGCCCCTGAG GCTGCAGCGC 
TCCCCCGTCC TCAAACGCAG GCCAAAACTC GAGGCACCTC CATCCCCAAG CCTAGGATCT 
GGCCTTGGAA CCGAGCCTCT GCCCCCACAG CCCACAGAGC CCTCCAGCCC TGAGCGGAGC 
CCACCCTCCC CAGCCACAGA CCAAAGAGGC GGCGGCCCCA ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013860.1
CDS	308..4615
Translation	MAQTPDGISCELRGEITRFLWPKEVELLLKTWLPGEGAVQNHVLALLRWRAYLLHTTCLPLRVDCTFSYLEVQAMALQETPPQVTFELESLRELVLEFPGVAALEQLAQHVAAAIKKVFPRSTLGKLFRRPTPASMLARLERSSPSESTDPCSPCGGFLETYEALCDYNGFPFREEIQWDVDTIYHRQGCRHFSLGDFSHLGSRDLALSVAALSYNLWFRCLSCVDMKLSLEVSEQILHMMSQSSHLEELVLETCSLRGDFVRRLAQALAGHSSSGLRELSLAGNLLDDRGMTALSRHLERCPGALRRLSLAQTGLTPRGMRALGRALATNAAFDSTLTHLDLSGNPGALGASEDSGGLYSFLSRPNVLSFLNLAGTDTALDTVRGCSVGGWMTGRADWRAGRGGLGPPAGVANSLPPQLFAAVSRGCCTSLTHLDASRNVFSRTKSRAAPAALQLFLSRARTLRHLGLAGCKLPPDALRALLDGLALNTHLRDLHLDLSACELRSAGAQVIQDLVCDAGAVSSLDLADNGFGSDMVTLVLAIGRSRSLRHVALGRNFNVRCKETLDDVLHRIVQLMQDDDCPLQSLSVAESRLKLGASVLLRALATNPNLTALDISGNAMGDAGAKLLAKALRVNSRLRSVVWDRNHTSALGLLDVAQALEQNHSLKAMPLPLNDVAQAQRSRPELTARAVHQIQACLLRNNRADPASSDHTTRLQPLGLVSDPSEQEVNELCQSVQEHVELLGCGAGPQGEAAVRQAEDAIQNANFSLSILPILYEAGSSPSHHWQLGQKLEGLLRQVGEVCRQDIQDFTQATLDTARSLCPQMLQGSSWREQLEGVLAGSRGLPELLPEQLLQDAFTRLRDMRLSITGTLAESIVAQALAGLSAARDQLVESLAQQATVTMPPALPAPDGGEPSLLEPGELEGLFFPEEKEEEKEKDDSPPQKWPELSHGLHLVPFIHSAAEEAEPEPELAAPGEDAEPQAGPSARGSPSPAAPGPPAGPLPRMDLPLAGQPLRHPTRARPRPRRQHHHRPPPGGPQVPPALPQEGNGLSARVDEGVEEFFSKRLIQQDRLWAPEEDPATEGGATPVPRTLRKKLGTLFAFKKPRSTRGPRTDLETSPGAAPRTRKTTFGDLLRPPTRPSRGEELGGAEGDTSSPDPAGRSRPRYTRDSKAYSMILLPAEEEATLGARPDKRRPLERGETELAPSFEQRVQVMLQRIGVSRGSGGAEGKRKQSKDGEIKKAGSDGDIMDSSTEAPPISIKSRTHSVSADPSCRPGPGSQGPESATWKTLGQQLNAELRSRGWGQQDGPGPPSPGQSPSPCRTSPSPDSLGLPEDPCLGPRNEDGQLRPRPLSAGRRAVSVHEDQLQAPAERPLRLQRSPVLKRRPKLEAPPSPSLGSGLGTEPLPPQPTEPSSPERSPPSPATDQRGGGPNP

Target ORF information:

RefSeq Version	NM_001013838.2
Organism	Homo sapiens(human)
Definition	Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013838.2

ORF Insert Sequence:

ATGGCCCAGA CCCCCGACGG CATCTCCTGT GAGCTCCGAG GCGAGATCAC CAGGTTCCTG 
TGGCCCAAAG AGGTGGAGCT GCTGCTGAAA ACCTGGCTAC CCGGGGAGGG TGCTGTGCAA 
AACCATGTCC TGGCACTGCT ACGATGGAGA GCCTACCTGC TGCACACCAC CTGCCTCCCG 
CTGAGGGTGG ACTGCACGTT CAGCTACCTG GAGGTCCAGG CCATGGCGCT GCAGGAGACA 
CCCCCTCAGG TCACCTTTGA GCTGGAGTCC CTGCGTGAGC TGGTCCTGGA GTTTCCTGGT 
GTGGCCGCCC TGGAACAGCT GGCCCAGCAC GTGGCTGCAG CCATCAAGAA GGTCTTCCCT 
CGCTCGACCC TTGGGAAGCT ATTCCGGAGG CCCACACCAG CCTCCATGCT GGCTCGGCTG 
GAGAGAAGCA GCCCCTCGGA GTCCACTGAC CCCTGCAGCC CCTGTGGTGG CTTCTTGGAG 
ACATACGAGG CTCTGTGTGA CTACAATGGC TTCCCTTTCC GAGAGGAGAT TCAGTGGGAC 
GTGGACACCA TTTACCATCG CCAGGGCTGC CGCCATTTCA GCCTGGGAGA CTTCAGCCAC 
CTCGGCAGTC GGGACCTGGC CTTGAGTGTG GCTGCCCTGT CCTACAACCT GTGGTTCCGG 
TGCCTCTCCT GTGTGGACAT GAAGCTGAGC CTTGAGGTCT CAGAACAGAT TCTGCACATG 
ATGAGTCAGT CATCACACCT GGAGGAGCTG GTGCTGGAGA CCTGCAGCCT GAGGGGAGAC 
TTTGTCCGAC GACTGGCCCA GGCGCTGGCG GGACACTCAA GCTCTGGGCT GCGGGAGCTC 
AGCCTCGCGG GGAACCTGCT GGATGACCGA GGCATGACTG CACTCAGCAG ACACCTCGAG 
CGTTGTCCAG GAGCCCTGAG GAGACTCAGC CTGGCCCAGA CAGGGTTGAC ACCGCGAGGA 
ATGAGGGCTC TGGGCCGGGC ACTGGCCACC AATGCCGCCT TCGACTCCAC CCTGACCCAC 
CTGGACCTTT CTGGGAATCC TGGGGCGCTG GGGGCCTCCG AGGACAGTGG GGGCCTCTAT 
AGCTTCCTGA GCCGTCCTAA CGTACTGTCG TTCCTGAATC TCGCAGGCAC CGACACTGCC 
CTGGACACTG TGAGGGGGTG CTCCGTGGGG GGATGGATGA CCGGCAGGGC GGACTGGAGG 
GCGGGACGGG GAGGGCTCGG TCCCCCCGCG GGTGTAGCCA ACAGCCTCCC CCCGCAGCTC 
TTCGCAGCGG TATCCCGAGG CTGCTGCACC AGCCTTACCC ACCTCGACGC TTCGAGGAAC 
GTCTTCTCCC GCACGAAGTC CCGCGCCGCG CCGGCCGCGC TGCAGCTCTT CCTCAGCCGC 
GCGCGGACGC TGCGGCACCT GGGCCTGGCG GGCTGCAAGC TGCCGCCCGA CGCGCTCAGA 
GCCCTTTTGG ATGGCCTCGC GCTCAACACG CACCTCCGCG ACCTGCACCT GGACCTCAGC 
GCTTGCGAGC TGCGCTCGGC CGGCGCCCAG GTGATACAAG ACTTAGTGTG CGACGCAGGC 
GCTGTGAGCT CCCTGGATCT GGCGGATAAC GGCTTCGGCT CAGACATGGT GACTCTGGTG 
CTGGCCATCG GGAGAAGCCG GTCCCTGAGA CATGTGGCGC TTGGAAGGAA CTTCAACGTC 
CGGTGCAAGG AGACCCTGGA CGACGTCCTG CACCGGATTG TCCAGCTCAT GCAGGACGAC 
GATTGTCCTC TTCAGTCTCT GTCGGTGGCT GAGTCTCGGC TGAAGCTGGG TGCCAGCGTC 
CTACTCCGGG CCCTAGCCAC CAATCCTAAC CTGACCGCGC TGGATATCAG CGGCAACGCC 
ATGGGGGACG CGGGCGCCAA GTTGCTGGCC AAGGCGCTGC GGGTCAACTC GAGGCTCCGG 
TCTGTGGTCT GGGACCGGAA CCACACATCT GCTTTGGGTC TGCTGGACGT GGCGCAGGCG 
CTGGAGCAGA ACCACAGCCT GAAGGCCATG CCTCTGCCAC TGAACGACGT GGCCCAGGCG 
CAGCGCAGCC GCCCGGAACT GACAGCACGT GCAGTCCATC AGATCCAAGC CTGTCTCTTG 
AGGAACAACC GCGCAGACCC TGCCTCTTCT GACCACACGA CCCGCCTTCA GCCACTTGGT 
CTGGTCTCAG ACCCCTCAGA GCAGGAAGTG AATGAATTGT GTCAGTCGGT GCAGGAGCAT 
GTGGAGCTGC TGGGCTGTGG GGCTGGGCCC CAGGGTGAAG CCGCTGTGCG CCAGGCCGAG 
GATGCCATCC AAAATGCCAA CTTCTCTCTC AGCATTCTCC CCATTCTATA TGAAGCTGGA 
AGCTCCCCAA GCCATCACTG GCAGCTTGGG CAGAAGCTGG AGGGCCTTCT GAGACAGGTG 
GGCGAGGTCT GCCGCCAGGA CATCCAGGAC TTCACTCAGG CCACACTGGA CACAGCAAGG 
AGCCTCTGCC CACAGATGCT GCAGGGATCC AGCTGGAGGG AGCAGCTAGA GGGGGTCCTG 
GCAGGCTCGA GGGGCCTCCC GGAGCTGCTC CCAGAGCAGC TGCTGCAAGA TGCCTTCACT 
AGGCTCAGGG ACATGCGGCT ATCAATCACG GGGACCTTGG CAGAGAGCAT TGTGGCTCAG 
GCTTTGGCAG GCCTGAGTGC AGCCCGGGAT CAGCTGGTGG AGAGTCTGGC TCAGCAGGCA 
ACAGTGACAA TGCCCCCTGC CCTACCAGCA CCGGATGGAG GTGAGCCCAG CCTCCTTGAG 
CCTGGGGAAT TGGAAGGTCT TTTCTTCCCC GAGGAGAAGG AAGAGGAGAA GGAGAAGGAT 
GACAGTCCTC CACAGAAATG GCCTGAGCTC AGCCACGGTC TTCACCTGGT CCCCTTCATT 
CACAGTGCTG CTGAGGAAGC GGAGCCGGAG CCCGAGCTGG CGGCTCCGGG AGAAGATGCA 
GAGCCGCAGG CGGGGCCGTC CGCGCGCGGC TCTCCGAGCC CTGCCGCCCC TGGGCCCCCG 
GCCGGCCCGC TGCCCCGCAT GGACCTGCCA CTGGCGGGGC AGCCCCTGCG CCATCCGACC 
CGGGCCCGGC CGCGGCCGCG CCGCCAGCAC CACCACCGCC CGCCGCCGGG GGGCCCCCAG 
GTACCCCCAG CCTTGCCGCA GGAAGGGAAT GGGCTCAGTG CCCGCGTGGA CGAGGGCGTG 
GAGGAATTCT TCTCCAAAAG GCTGATCCAG CAGGATCGCC TCTGGGCCCC CGAGGAGGAC 
CCGGCCACTG AGGGGGGCGC CACTCCTGTC CCCCGTACAC TGCGAAAGAA GCTGGGCACC 
CTCTTTGCCT TCAAGAAGCC TCGTTCAACG CGGGGTCCAC GGACTGATCT AGAGACCAGC 
CCTGGGGCAG CTCCCCGAAC CCGAAAAACT ACATTTGGCG ACCTACTGCG GCCGCCAACC 
CGTCCCAGCC GTGGTGAGGA GCTTGGTGGG GCTGAGGGGG ACACCAGCAG CCCTGACCCT 
GCCGGCAGGA GCCGACCTCG CTACACAAGA GATAGCAAGG CCTACTCGAT GATACTGCTG 
CCTGCCGAGG AGGAGGCAAC GCTGGGTGCC AGACCCGACA AGCGGCGGCC CCTGGAGCGG 
GGAGAAACAG AACTGGCTCC ATCCTTTGAA CAGCGGGTAC AAGTAATGCT GCAGAGGATA 
GGCGTCAGCC GAGGCAGCGG GGGTGCCGAA GGCAAGAGGA AGCAAAGCAA AGATGGCGAG 
ATCAAGAAAG CTGGCTCAGA TGGTGACATT ATGGACAGTT CCACGGAGGC CCCTCCCATC 
TCGATCAAGT CCCGCACCCA CTCTGTGTCT GCTGACCCTT CCTGCAGACC TGGCCCAGGG 
AGCCAGGGGC CTGAGTCTGC CACCTGGAAG ACACTGGGGC AGCAGTTGAA TGCGGAGCTC 
AGGAGCCGTG GTTGGGGCCA ACAGGATGGT CCAGGCCCTC CCTCCCCTGG TCAAAGCCCA 
AGTCCCTGCA GAACCAGCCC CTCCCCAGAC AGCCTGGGCC TCCCAGAGGA CCCTTGCTTG 
GGCCCCAGAA ATGAAGATGG CCAGCTGAGG CCGAGGCCTC TCTCGGCAGG GCGGCGAGCA 
GTGTCTGTGC ATGAGGACCA GCTCCAGGCC CCTGCTGAAC GGCCCCTGAG GCTGCAGCGC 
TCCCCCGTCC TCAAACGCAG GCCAAAACTC GAGGCACCTC CATCCCCAAG CCTAGGATCT 
GGCCTTGGAA CCGAGCCTCT GCCCCCACAG CCCACAGAGC CCTCCAGCCC TGAGCGGAGC 
CCACCCTCCC CAGCCACAGA CCAAAGAGGC GGCGGCCCCA ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CARMIL2 ( NM_001317026.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303955.1 Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu49273
Clone ID Related Accession (Same CDS sequence)	NM_001317026.3 , NM_001317026.2
Accession Version	NM_001317026.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	capping protein, Arp2/3 and myosin-I linker protein 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB113647.1 and FJ026014.1. On May 31, 2019 this sequence version replaced NM_001317026.2. Transcript Variant: This variant (2) represents the shorter transcript and encodes the shorter isoform (2, also known as CARMIL2b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ026014.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAGA CCCCCGACGG CATCTCCTGT GAGCTCCGAG GCGAGATCAC CAGGTTCCTG 
TGGCCCAAAG AGGTGGAGCT GCTGCTGAAA ACCTGGCTAC CCGGGGAGGG TGCTGTGCAA 
AACCATGTCC TGGCACTGCT ACGATGGAGA GCCTACCTGC TGCACACCAC CTGCCTCCCG 
CTGAGGGTGG ACTGCACGTT CAGCTACCTG GAGGTCCAGG CCATGGCGCT GCAGGAGACA 
CCCCCTCAGG TCACCTTTGA GCTGGAGTCC CTGCGTGAGC TGGTCCTGGA GTTTCCTGGT 
GTGGCCGCCC TGGAACAGCT GGCCCAGCAC GTGGCTGCAG CCATCAAGAA GGTCTTCCCT 
CGCTCGACCC TTGGGAAGCT ATTCCGGAGG CCCACACCAG CCTCCATGCT GGCTCGGCTG 
GAGAGAAGCA GCCCCTCGGA GTCCACTGAC CCCTGCAGCC CCTGTGGTGG CTTCTTGGAG 
ACATACGAGG CTCTGTGTGA CTACAATGGC TTCCCTTTCC GAGAGGAGAT TCAGTGGGAC 
GTGGACACCA TTTACCATCG CCAGGGCTGC CGCCATTTCA GCCTGGGAGA CTTCAGCCAC 
CTCGGCAGTC GGGACCTGGC CTTGAGTGTG GCTGCCCTGT CCTACAACCT GTGGTTCCGG 
TGCCTCTCCT GTGTGGACAT GAAGCTGAGC CTTGAGGTCT CAGAACAGAT TCTGCACATG 
ATGAGTCAGT CATCACACCT GGAGGAGCTG GTGCTGGAGA CCTGCAGCCT GAGGGGAGAC 
TTTGTCCGAC GACTGGCCCA GGCGCTGGCG GGACACTCAA GCTCTGGGCT GCGGGAGCTC 
AGCCTCGCGG GGAACCTGCT GGATGACCGA GGCATGACTG CACTCAGCAG ACACCTCGAG 
CGTTGTCCAG GAGCCCTGAG GAGACTCAGC CTGGCCCAGA CAGGGTTGAC ACCGCGAGGA 
ATGAGGGCTC TGGGCCGGGC ACTGGCCACC AATGCCGCCT TCGACTCCAC CCTGACCCAC 
CTGGACCTTT CTGGGAATCC TGGGGCGCTG GGGGCCTCCG AGGACAGTGG GGGCCTCTAT 
AGCTTCCTGA GCCGTCCTAA CGTACTGTCG TTCCTGAATC TCGCAGGCAC CGACACTGCC 
CTGGACACTC TCTTCGCAGC GGTATCCCGA GGCTGCTGCA CCAGCCTTAC CCACCTCGAC 
GCTTCGAGGA ACGTCTTCTC CCGCACGAAG TCCCGCGCCG CGCCGGCCGC GCTGCAGCTC 
TTCCTCAGCC GCGCGCGGAC GCTGCGGCAC CTGGGCCTGG CGGGCTGCAA GCTGCCGCCC 
GACGCGCTCA GAGCCCTTTT GGATGGCCTC GCGCTCAACA CGCACCTCCG CGACCTGCAC 
CTGGACCTCA GCGCTTGCGA GCTGCGCTCG GCCGGCGCCC AGGTGATACA AGACTTAGTG 
TGCGACGCAG GCGCTGTGAG CTCCCTGGAT CTGGCGGATA ACGGCTTCGG CTCAGACATG 
GTGACTCTGG TGCTGGCCAT CGGGAGAAGC CGGTCCCTGA GACATGTGGC GCTTGGAAGG 
AACTTCAACG TCCGGTGCAA GGAGACCCTG GACGACGTCC TGCACCGGAT TGTCCAGCTC 
ATGCAGGACG ACGATTGTCC TCTTCAGTCT CTGTCGGTGG CTGAGTCTCG GCTGAAGCTG 
GGTGCCAGCG TCCTACTCCG GGCCCTAGCC ACCAATCCTA ACCTGACCGC GCTGGATATC 
AGCGGCAACG CCATGGGGGA CGCGGGCGCC AAGTTGCTGG CCAAGGCGCT GCGGGTCAAC 
TCGAGGCTCC GGTCTGTGGT CTGGGACCGG AACCACACAT CTGCTTTGGG TCTGCTGGAC 
GTGGCGCAGG CGCTGGAGCA GAACCACAGC CTGAAGGCCA TGCCTCTGCC ACTGAACGAC 
GTGGCCCAGG CGCAGCGCAG CCGCCCGGAA CTGACAGCAC GTGCAGTCCA TCAGATCCAA 
GCCTGTCTCT TGAGGAACAA CCGCGCAGAC CCTGCCTCTT CTGACCACAC GACCCGCCTT 
CAGCCACTTG GTCTGGTCTC AGACCCCTCA GAGCAGGAAG TGAATGAATT GTGTCAGTCG 
GTGCAGGAGC ATGTGGAGCT GCTGGGCTGT GGGGCTGGGC CCCAGGGTGA AGCCGCTGTG 
CGCCAGGCCG AGGATGCCAT CCAAAATGCC AACTTCTCTC TCAGCATTCT CCCCATTCTA 
TATGAAGCTG GAAGCTCCCC AAGCCATCAC TGGCAGCTTG GGCAGAAGCT GGAGGGCCTT 
CTGAGACAGG TGGGCGAGGT CTGCCGCCAG GACATCCAGG ACTTCACTCA GGCCACACTG 
GACACAGCAA GGAGCCTCTG CCCACAGATG CTGCAGGGAT CCAGCTGGAG GGAGCAGCTA 
GAGGGGGTCC TGGCAGGCTC GAGGGGCCTC CCGGAGCTGC TCCCAGAGCA GCTGCTGCAA 
GATGCCTTCA CTAGGCTCAG GGACATGCGG CTATCAATCA CGGGGACCTT GGCAGAGAGC 
ATTGTGGCTC AGGCTTTGGC AGGCCTGAGT GCAGCCCGGG ATCAGCTGGT GGAGAGTCTG 
GCTCAGCAGG CAACAGTGAC AATGCCCCCT GCCCTACCAG CACCGGATGG AGGTGAGCCC 
AGCCTCCTTG AGCCTGGGGA ATTGGAAGGT CTTTTCTTCC CCGAGGAGAA GGAAGAGGAG 
AAGGAGAAGG ATGACAGTCC TCCACAGAAA TGGCCTGAGC TCAGCCACGG TCTTCACCTG 
GTCCCCTTCA TTCACAGTGC TGCTGAGGAA GCGGAGCCGG AGCCCGAGCT GGCGGCTCCG 
GGAGAAGATG CAGAGCCGCA GGCGGGGCCG TCCGCGCGCG GCTCTCCGAG CCCTGCCGCC 
CCTGGGCCCC CGGCCGGCCC GCTGCCCCGC ATGGACCTGC CACTGGCGGG GCAGCCCCTG 
CGCCATCCGA CCCGGGCCCG GCCGCGGCCG CGCCGCCAGC ACCACCACCG CCCGCCGCCG 
GGGGGCCCCC AGGTACCCCC AGCCTTGCCG CAGGAAGGGA ATGGGCTCAG TGCCCGCGTG 
GACGAGGGCG TGGAGGAATT CTTCTCCAAA AGGCTGATCC AGCAGGATCG CCTCTGGGCC 
CCCGAGGAGG ACCCGGCCAC TGAGGGGGGC GCCACTCCTG TCCCCCGTAC ACTGCGAAAG 
AAGCTGGGCA CCCTCTTTGC CTTCAAGAAG CCTCGTTCAA CGCGGGGTCC ACGGACTGAT 
CTAGAGACCA GCCCTGGGGC AGCTCCCCGA ACCCGAAAAA CTACATTTGG CGACCTACTG 
CGGCCGCCAA CCCGTCCCAG CCGTGGTGAG GAGCTTGGTG GGGCTGAGGG GGACACCAGC 
AGCCCTGACC CTGCCGGCAG GAGCCGACCT CGCTACACAA GAGATAGCAA GGCCTACTCG 
ATGATACTGC TGCCTGCCGA GGAGGAGGCA ACGCTGGGTG CCAGACCCGA CAAGCGGCGG 
CCCCTGGAGC GGGGAGAAAC AGAACTGGCT CCATCCTTTG AACAGCGGGT ACAAGTAATG 
CTGCAGAGGA TAGGCGTCAG CCGAGGCAGC GGGGGTGCCG AAGGCAAGAG GAAGCAAAGC 
AAAGATGGCG AGATCAAGAA AGCTGGCTCA GATGGTGACA TTATGGACAG TTCCACGGAG 
GCCCCTCCCA TCTCGATCAA GTCCCGCACC CACTCTGTGT CTGCTGACCC TTCCTGCAGA 
CCTGGCCCAG GGAGCCAGGG GCCTGAGTCT GCCACCTGGA AGACACTGGG GCAGCAGTTG 
AATGCGGAGC TCAGGAGCCG TGGTTGGGGC CAACAGGATG GTCCAGGCCC TCCCTCCCCT 
GGTCAAAGCC CAAGTCCCTG CAGAACCAGC CCCTCCCCAG ACAGCCTGGG CCTCCCAGAG 
GACCCTTGCT TGGGCCCCAG AAATGAAGAA CGGCCCCTGA GGCTGCAGCG CTCCCCCGTC 
CTCAAACGCA GGCCAAAACT CGAGGCACCT CCATCCCCAA GCCTAGGATC TGGCCTTGGA 
ACCGAGCCTC TGCCCCCACA GCCCACAGAG CCCTCCAGCC CTGAGCGGAG CCCACCCTCC 
CCAGCCACAG ACCAAAGAGG CGGCGGCCCC AATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303955.1
CDS	104..4222
Translation	MAQTPDGISCELRGEITRFLWPKEVELLLKTWLPGEGAVQNHVLALLRWRAYLLHTTCLPLRVDCTFSYLEVQAMALQETPPQVTFELESLRELVLEFPGVAALEQLAQHVAAAIKKVFPRSTLGKLFRRPTPASMLARLERSSPSESTDPCSPCGGFLETYEALCDYNGFPFREEIQWDVDTIYHRQGCRHFSLGDFSHLGSRDLALSVAALSYNLWFRCLSCVDMKLSLEVSEQILHMMSQSSHLEELVLETCSLRGDFVRRLAQALAGHSSSGLRELSLAGNLLDDRGMTALSRHLERCPGALRRLSLAQTGLTPRGMRALGRALATNAAFDSTLTHLDLSGNPGALGASEDSGGLYSFLSRPNVLSFLNLAGTDTALDTLFAAVSRGCCTSLTHLDASRNVFSRTKSRAAPAALQLFLSRARTLRHLGLAGCKLPPDALRALLDGLALNTHLRDLHLDLSACELRSAGAQVIQDLVCDAGAVSSLDLADNGFGSDMVTLVLAIGRSRSLRHVALGRNFNVRCKETLDDVLHRIVQLMQDDDCPLQSLSVAESRLKLGASVLLRALATNPNLTALDISGNAMGDAGAKLLAKALRVNSRLRSVVWDRNHTSALGLLDVAQALEQNHSLKAMPLPLNDVAQAQRSRPELTARAVHQIQACLLRNNRADPASSDHTTRLQPLGLVSDPSEQEVNELCQSVQEHVELLGCGAGPQGEAAVRQAEDAIQNANFSLSILPILYEAGSSPSHHWQLGQKLEGLLRQVGEVCRQDIQDFTQATLDTARSLCPQMLQGSSWREQLEGVLAGSRGLPELLPEQLLQDAFTRLRDMRLSITGTLAESIVAQALAGLSAARDQLVESLAQQATVTMPPALPAPDGGEPSLLEPGELEGLFFPEEKEEEKEKDDSPPQKWPELSHGLHLVPFIHSAAEEAEPEPELAAPGEDAEPQAGPSARGSPSPAAPGPPAGPLPRMDLPLAGQPLRHPTRARPRPRRQHHHRPPPGGPQVPPALPQEGNGLSARVDEGVEEFFSKRLIQQDRLWAPEEDPATEGGATPVPRTLRKKLGTLFAFKKPRSTRGPRTDLETSPGAAPRTRKTTFGDLLRPPTRPSRGEELGGAEGDTSSPDPAGRSRPRYTRDSKAYSMILLPAEEEATLGARPDKRRPLERGETELAPSFEQRVQVMLQRIGVSRGSGGAEGKRKQSKDGEIKKAGSDGDIMDSSTEAPPISIKSRTHSVSADPSCRPGPGSQGPESATWKTLGQQLNAELRSRGWGQQDGPGPPSPGQSPSPCRTSPSPDSLGLPEDPCLGPRNEERPLRLQRSPVLKRRPKLEAPPSPSLGSGLGTEPLPPQPTEPSSPERSPPSPATDQRGGGPNP

Target ORF information:

RefSeq Version	NM_001317026.3
Organism	Homo sapiens(human)
Definition	Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317026.3

ORF Insert Sequence:

ATGGCCCAGA CCCCCGACGG CATCTCCTGT GAGCTCCGAG GCGAGATCAC CAGGTTCCTG 
TGGCCCAAAG AGGTGGAGCT GCTGCTGAAA ACCTGGCTAC CCGGGGAGGG TGCTGTGCAA 
AACCATGTCC TGGCACTGCT ACGATGGAGA GCCTACCTGC TGCACACCAC CTGCCTCCCG 
CTGAGGGTGG ACTGCACGTT CAGCTACCTG GAGGTCCAGG CCATGGCGCT GCAGGAGACA 
CCCCCTCAGG TCACCTTTGA GCTGGAGTCC CTGCGTGAGC TGGTCCTGGA GTTTCCTGGT 
GTGGCCGCCC TGGAACAGCT GGCCCAGCAC GTGGCTGCAG CCATCAAGAA GGTCTTCCCT 
CGCTCGACCC TTGGGAAGCT ATTCCGGAGG CCCACACCAG CCTCCATGCT GGCTCGGCTG 
GAGAGAAGCA GCCCCTCGGA GTCCACTGAC CCCTGCAGCC CCTGTGGTGG CTTCTTGGAG 
ACATACGAGG CTCTGTGTGA CTACAATGGC TTCCCTTTCC GAGAGGAGAT TCAGTGGGAC 
GTGGACACCA TTTACCATCG CCAGGGCTGC CGCCATTTCA GCCTGGGAGA CTTCAGCCAC 
CTCGGCAGTC GGGACCTGGC CTTGAGTGTG GCTGCCCTGT CCTACAACCT GTGGTTCCGG 
TGCCTCTCCT GTGTGGACAT GAAGCTGAGC CTTGAGGTCT CAGAACAGAT TCTGCACATG 
ATGAGTCAGT CATCACACCT GGAGGAGCTG GTGCTGGAGA CCTGCAGCCT GAGGGGAGAC 
TTTGTCCGAC GACTGGCCCA GGCGCTGGCG GGACACTCAA GCTCTGGGCT GCGGGAGCTC 
AGCCTCGCGG GGAACCTGCT GGATGACCGA GGCATGACTG CACTCAGCAG ACACCTCGAG 
CGTTGTCCAG GAGCCCTGAG GAGACTCAGC CTGGCCCAGA CAGGGTTGAC ACCGCGAGGA 
ATGAGGGCTC TGGGCCGGGC ACTGGCCACC AATGCCGCCT TCGACTCCAC CCTGACCCAC 
CTGGACCTTT CTGGGAATCC TGGGGCGCTG GGGGCCTCCG AGGACAGTGG GGGCCTCTAT 
AGCTTCCTGA GCCGTCCTAA CGTACTGTCG TTCCTGAATC TCGCAGGCAC CGACACTGCC 
CTGGACACTC TCTTCGCAGC GGTATCCCGA GGCTGCTGCA CCAGCCTTAC CCACCTCGAC 
GCTTCGAGGA ACGTCTTCTC CCGCACGAAG TCCCGCGCCG CGCCGGCCGC GCTGCAGCTC 
TTCCTCAGCC GCGCGCGGAC GCTGCGGCAC CTGGGCCTGG CGGGCTGCAA GCTGCCGCCC 
GACGCGCTCA GAGCCCTTTT GGATGGCCTC GCGCTCAACA CGCACCTCCG CGACCTGCAC 
CTGGACCTCA GCGCTTGCGA GCTGCGCTCG GCCGGCGCCC AGGTGATACA AGACTTAGTG 
TGCGACGCAG GCGCTGTGAG CTCCCTGGAT CTGGCGGATA ACGGCTTCGG CTCAGACATG 
GTGACTCTGG TGCTGGCCAT CGGGAGAAGC CGGTCCCTGA GACATGTGGC GCTTGGAAGG 
AACTTCAACG TCCGGTGCAA GGAGACCCTG GACGACGTCC TGCACCGGAT TGTCCAGCTC 
ATGCAGGACG ACGATTGTCC TCTTCAGTCT CTGTCGGTGG CTGAGTCTCG GCTGAAGCTG 
GGTGCCAGCG TCCTACTCCG GGCCCTAGCC ACCAATCCTA ACCTGACCGC GCTGGATATC 
AGCGGCAACG CCATGGGGGA CGCGGGCGCC AAGTTGCTGG CCAAGGCGCT GCGGGTCAAC 
TCGAGGCTCC GGTCTGTGGT CTGGGACCGG AACCACACAT CTGCTTTGGG TCTGCTGGAC 
GTGGCGCAGG CGCTGGAGCA GAACCACAGC CTGAAGGCCA TGCCTCTGCC ACTGAACGAC 
GTGGCCCAGG CGCAGCGCAG CCGCCCGGAA CTGACAGCAC GTGCAGTCCA TCAGATCCAA 
GCCTGTCTCT TGAGGAACAA CCGCGCAGAC CCTGCCTCTT CTGACCACAC GACCCGCCTT 
CAGCCACTTG GTCTGGTCTC AGACCCCTCA GAGCAGGAAG TGAATGAATT GTGTCAGTCG 
GTGCAGGAGC ATGTGGAGCT GCTGGGCTGT GGGGCTGGGC CCCAGGGTGA AGCCGCTGTG 
CGCCAGGCCG AGGATGCCAT CCAAAATGCC AACTTCTCTC TCAGCATTCT CCCCATTCTA 
TATGAAGCTG GAAGCTCCCC AAGCCATCAC TGGCAGCTTG GGCAGAAGCT GGAGGGCCTT 
CTGAGACAGG TGGGCGAGGT CTGCCGCCAG GACATCCAGG ACTTCACTCA GGCCACACTG 
GACACAGCAA GGAGCCTCTG CCCACAGATG CTGCAGGGAT CCAGCTGGAG GGAGCAGCTA 
GAGGGGGTCC TGGCAGGCTC GAGGGGCCTC CCGGAGCTGC TCCCAGAGCA GCTGCTGCAA 
GATGCCTTCA CTAGGCTCAG GGACATGCGG CTATCAATCA CGGGGACCTT GGCAGAGAGC 
ATTGTGGCTC AGGCTTTGGC AGGCCTGAGT GCAGCCCGGG ATCAGCTGGT GGAGAGTCTG 
GCTCAGCAGG CAACAGTGAC AATGCCCCCT GCCCTACCAG CACCGGATGG AGGTGAGCCC 
AGCCTCCTTG AGCCTGGGGA ATTGGAAGGT CTTTTCTTCC CCGAGGAGAA GGAAGAGGAG 
AAGGAGAAGG ATGACAGTCC TCCACAGAAA TGGCCTGAGC TCAGCCACGG TCTTCACCTG 
GTCCCCTTCA TTCACAGTGC TGCTGAGGAA GCGGAGCCGG AGCCCGAGCT GGCGGCTCCG 
GGAGAAGATG CAGAGCCGCA GGCGGGGCCG TCCGCGCGCG GCTCTCCGAG CCCTGCCGCC 
CCTGGGCCCC CGGCCGGCCC GCTGCCCCGC ATGGACCTGC CACTGGCGGG GCAGCCCCTG 
CGCCATCCGA CCCGGGCCCG GCCGCGGCCG CGCCGCCAGC ACCACCACCG CCCGCCGCCG 
GGGGGCCCCC AGGTACCCCC AGCCTTGCCG CAGGAAGGGA ATGGGCTCAG TGCCCGCGTG 
GACGAGGGCG TGGAGGAATT CTTCTCCAAA AGGCTGATCC AGCAGGATCG CCTCTGGGCC 
CCCGAGGAGG ACCCGGCCAC TGAGGGGGGC GCCACTCCTG TCCCCCGTAC ACTGCGAAAG 
AAGCTGGGCA CCCTCTTTGC CTTCAAGAAG CCTCGTTCAA CGCGGGGTCC ACGGACTGAT 
CTAGAGACCA GCCCTGGGGC AGCTCCCCGA ACCCGAAAAA CTACATTTGG CGACCTACTG 
CGGCCGCCAA CCCGTCCCAG CCGTGGTGAG GAGCTTGGTG GGGCTGAGGG GGACACCAGC 
AGCCCTGACC CTGCCGGCAG GAGCCGACCT CGCTACACAA GAGATAGCAA GGCCTACTCG 
ATGATACTGC TGCCTGCCGA GGAGGAGGCA ACGCTGGGTG CCAGACCCGA CAAGCGGCGG 
CCCCTGGAGC GGGGAGAAAC AGAACTGGCT CCATCCTTTG AACAGCGGGT ACAAGTAATG 
CTGCAGAGGA TAGGCGTCAG CCGAGGCAGC GGGGGTGCCG AAGGCAAGAG GAAGCAAAGC 
AAAGATGGCG AGATCAAGAA AGCTGGCTCA GATGGTGACA TTATGGACAG TTCCACGGAG 
GCCCCTCCCA TCTCGATCAA GTCCCGCACC CACTCTGTGT CTGCTGACCC TTCCTGCAGA 
CCTGGCCCAG GGAGCCAGGG GCCTGAGTCT GCCACCTGGA AGACACTGGG GCAGCAGTTG 
AATGCGGAGC TCAGGAGCCG TGGTTGGGGC CAACAGGATG GTCCAGGCCC TCCCTCCCCT 
GGTCAAAGCC CAAGTCCCTG CAGAACCAGC CCCTCCCCAG ACAGCCTGGG CCTCCCAGAG 
GACCCTTGCT TGGGCCCCAG AAATGAAGAA CGGCCCCTGA GGCTGCAGCG CTCCCCCGTC 
CTCAAACGCA GGCCAAAACT CGAGGCACCT CCATCCCCAA GCCTAGGATC TGGCCTTGGA 
ACCGAGCCTC TGCCCCCACA GCCCACAGAG CCCTCCAGCC CTGAGCGGAG CCCACCCTCC 
CCAGCCACAG ACCAAAGAGG CGGCGGCCCC AATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CARMIL2 ( NM_001317026.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303955.1 Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant 2, mRNA.

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CloneID	OHu49273
Clone ID Related Accession (Same CDS sequence)	NM_001317026.3 , NM_001317026.2
Accession Version	NM_001317026.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-17
Organism	Homo sapiens(human)
Product	capping protein, Arp2/3 and myosin-I linker protein 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009095.7, AB113647.1 and FJ026014.1. On May 4, 2017 this sequence version replaced NM_001317026.1. Transcript Variant: This variant (2) represents the shorter transcript and encodes the shorter isoform (2, also known as CARMIL2b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ026014.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAGA CCCCCGACGG CATCTCCTGT GAGCTCCGAG GCGAGATCAC CAGGTTCCTG 
TGGCCCAAAG AGGTGGAGCT GCTGCTGAAA ACCTGGCTAC CCGGGGAGGG TGCTGTGCAA 
AACCATGTCC TGGCACTGCT ACGATGGAGA GCCTACCTGC TGCACACCAC CTGCCTCCCG 
CTGAGGGTGG ACTGCACGTT CAGCTACCTG GAGGTCCAGG CCATGGCGCT GCAGGAGACA 
CCCCCTCAGG TCACCTTTGA GCTGGAGTCC CTGCGTGAGC TGGTCCTGGA GTTTCCTGGT 
GTGGCCGCCC TGGAACAGCT GGCCCAGCAC GTGGCTGCAG CCATCAAGAA GGTCTTCCCT 
CGCTCGACCC TTGGGAAGCT ATTCCGGAGG CCCACACCAG CCTCCATGCT GGCTCGGCTG 
GAGAGAAGCA GCCCCTCGGA GTCCACTGAC CCCTGCAGCC CCTGTGGTGG CTTCTTGGAG 
ACATACGAGG CTCTGTGTGA CTACAATGGC TTCCCTTTCC GAGAGGAGAT TCAGTGGGAC 
GTGGACACCA TTTACCATCG CCAGGGCTGC CGCCATTTCA GCCTGGGAGA CTTCAGCCAC 
CTCGGCAGTC GGGACCTGGC CTTGAGTGTG GCTGCCCTGT CCTACAACCT GTGGTTCCGG 
TGCCTCTCCT GTGTGGACAT GAAGCTGAGC CTTGAGGTCT CAGAACAGAT TCTGCACATG 
ATGAGTCAGT CATCACACCT GGAGGAGCTG GTGCTGGAGA CCTGCAGCCT GAGGGGAGAC 
TTTGTCCGAC GACTGGCCCA GGCGCTGGCG GGACACTCAA GCTCTGGGCT GCGGGAGCTC 
AGCCTCGCGG GGAACCTGCT GGATGACCGA GGCATGACTG CACTCAGCAG ACACCTCGAG 
CGTTGTCCAG GAGCCCTGAG GAGACTCAGC CTGGCCCAGA CAGGGTTGAC ACCGCGAGGA 
ATGAGGGCTC TGGGCCGGGC ACTGGCCACC AATGCCGCCT TCGACTCCAC CCTGACCCAC 
CTGGACCTTT CTGGGAATCC TGGGGCGCTG GGGGCCTCCG AGGACAGTGG GGGCCTCTAT 
AGCTTCCTGA GCCGTCCTAA CGTACTGTCG TTCCTGAATC TCGCAGGCAC CGACACTGCC 
CTGGACACTC TCTTCGCAGC GGTATCCCGA GGCTGCTGCA CCAGCCTTAC CCACCTCGAC 
GCTTCGAGGA ACGTCTTCTC CCGCACGAAG TCCCGCGCCG CGCCGGCCGC GCTGCAGCTC 
TTCCTCAGCC GCGCGCGGAC GCTGCGGCAC CTGGGCCTGG CGGGCTGCAA GCTGCCGCCC 
GACGCGCTCA GAGCCCTTTT GGATGGCCTC GCGCTCAACA CGCACCTCCG CGACCTGCAC 
CTGGACCTCA GCGCTTGCGA GCTGCGCTCG GCCGGCGCCC AGGTGATACA AGACTTAGTG 
TGCGACGCAG GCGCTGTGAG CTCCCTGGAT CTGGCGGATA ACGGCTTCGG CTCAGACATG 
GTGACTCTGG TGCTGGCCAT CGGGAGAAGC CGGTCCCTGA GACATGTGGC GCTTGGAAGG 
AACTTCAACG TCCGGTGCAA GGAGACCCTG GACGACGTCC TGCACCGGAT TGTCCAGCTC 
ATGCAGGACG ACGATTGTCC TCTTCAGTCT CTGTCGGTGG CTGAGTCTCG GCTGAAGCTG 
GGTGCCAGCG TCCTACTCCG GGCCCTAGCC ACCAATCCTA ACCTGACCGC GCTGGATATC 
AGCGGCAACG CCATGGGGGA CGCGGGCGCC AAGTTGCTGG CCAAGGCGCT GCGGGTCAAC 
TCGAGGCTCC GGTCTGTGGT CTGGGACCGG AACCACACAT CTGCTTTGGG TCTGCTGGAC 
GTGGCGCAGG CGCTGGAGCA GAACCACAGC CTGAAGGCCA TGCCTCTGCC ACTGAACGAC 
GTGGCCCAGG CGCAGCGCAG CCGCCCGGAA CTGACAGCAC GTGCAGTCCA TCAGATCCAA 
GCCTGTCTCT TGAGGAACAA CCGCGCAGAC CCTGCCTCTT CTGACCACAC GACCCGCCTT 
CAGCCACTTG GTCTGGTCTC AGACCCCTCA GAGCAGGAAG TGAATGAATT GTGTCAGTCG 
GTGCAGGAGC ATGTGGAGCT GCTGGGCTGT GGGGCTGGGC CCCAGGGTGA AGCCGCTGTG 
CGCCAGGCCG AGGATGCCAT CCAAAATGCC AACTTCTCTC TCAGCATTCT CCCCATTCTA 
TATGAAGCTG GAAGCTCCCC AAGCCATCAC TGGCAGCTTG GGCAGAAGCT GGAGGGCCTT 
CTGAGACAGG TGGGCGAGGT CTGCCGCCAG GACATCCAGG ACTTCACTCA GGCCACACTG 
GACACAGCAA GGAGCCTCTG CCCACAGATG CTGCAGGGAT CCAGCTGGAG GGAGCAGCTA 
GAGGGGGTCC TGGCAGGCTC GAGGGGCCTC CCGGAGCTGC TCCCAGAGCA GCTGCTGCAA 
GATGCCTTCA CTAGGCTCAG GGACATGCGG CTATCAATCA CGGGGACCTT GGCAGAGAGC 
ATTGTGGCTC AGGCTTTGGC AGGCCTGAGT GCAGCCCGGG ATCAGCTGGT GGAGAGTCTG 
GCTCAGCAGG CAACAGTGAC AATGCCCCCT GCCCTACCAG CACCGGATGG AGGTGAGCCC 
AGCCTCCTTG AGCCTGGGGA ATTGGAAGGT CTTTTCTTCC CCGAGGAGAA GGAAGAGGAG 
AAGGAGAAGG ATGACAGTCC TCCACAGAAA TGGCCTGAGC TCAGCCACGG TCTTCACCTG 
GTCCCCTTCA TTCACAGTGC TGCTGAGGAA GCGGAGCCGG AGCCCGAGCT GGCGGCTCCG 
GGAGAAGATG CAGAGCCGCA GGCGGGGCCG TCCGCGCGCG GCTCTCCGAG CCCTGCCGCC 
CCTGGGCCCC CGGCCGGCCC GCTGCCCCGC ATGGACCTGC CACTGGCGGG GCAGCCCCTG 
CGCCATCCGA CCCGGGCCCG GCCGCGGCCG CGCCGCCAGC ACCACCACCG CCCGCCGCCG 
GGGGGCCCCC AGGTACCCCC AGCCTTGCCG CAGGAAGGGA ATGGGCTCAG TGCCCGCGTG 
GACGAGGGCG TGGAGGAATT CTTCTCCAAA AGGCTGATCC AGCAGGATCG CCTCTGGGCC 
CCCGAGGAGG ACCCGGCCAC TGAGGGGGGC GCCACTCCTG TCCCCCGTAC ACTGCGAAAG 
AAGCTGGGCA CCCTCTTTGC CTTCAAGAAG CCTCGTTCAA CGCGGGGTCC ACGGACTGAT 
CTAGAGACCA GCCCTGGGGC AGCTCCCCGA ACCCGAAAAA CTACATTTGG CGACCTACTG 
CGGCCGCCAA CCCGTCCCAG CCGTGGTGAG GAGCTTGGTG GGGCTGAGGG GGACACCAGC 
AGCCCTGACC CTGCCGGCAG GAGCCGACCT CGCTACACAA GAGATAGCAA GGCCTACTCG 
ATGATACTGC TGCCTGCCGA GGAGGAGGCA ACGCTGGGTG CCAGACCCGA CAAGCGGCGG 
CCCCTGGAGC GGGGAGAAAC AGAACTGGCT CCATCCTTTG AACAGCGGGT ACAAGTAATG 
CTGCAGAGGA TAGGCGTCAG CCGAGGCAGC GGGGGTGCCG AAGGCAAGAG GAAGCAAAGC 
AAAGATGGCG AGATCAAGAA AGCTGGCTCA GATGGTGACA TTATGGACAG TTCCACGGAG 
GCCCCTCCCA TCTCGATCAA GTCCCGCACC CACTCTGTGT CTGCTGACCC TTCCTGCAGA 
CCTGGCCCAG GGAGCCAGGG GCCTGAGTCT GCCACCTGGA AGACACTGGG GCAGCAGTTG 
AATGCGGAGC TCAGGAGCCG TGGTTGGGGC CAACAGGATG GTCCAGGCCC TCCCTCCCCT 
GGTCAAAGCC CAAGTCCCTG CAGAACCAGC CCCTCCCCAG ACAGCCTGGG CCTCCCAGAG 
GACCCTTGCT TGGGCCCCAG AAATGAAGAA CGGCCCCTGA GGCTGCAGCG CTCCCCCGTC 
CTCAAACGCA GGCCAAAACT CGAGGCACCT CCATCCCCAA GCCTAGGATC TGGCCTTGGA 
ACCGAGCCTC TGCCCCCACA GCCCACAGAG CCCTCCAGCC CTGAGCGGAG CCCACCCTCC 
CCAGCCACAG ACCAAAGAGG CGGCGGCCCC AATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303955.1
CDS	308..4426
Translation	MAQTPDGISCELRGEITRFLWPKEVELLLKTWLPGEGAVQNHVLALLRWRAYLLHTTCLPLRVDCTFSYLEVQAMALQETPPQVTFELESLRELVLEFPGVAALEQLAQHVAAAIKKVFPRSTLGKLFRRPTPASMLARLERSSPSESTDPCSPCGGFLETYEALCDYNGFPFREEIQWDVDTIYHRQGCRHFSLGDFSHLGSRDLALSVAALSYNLWFRCLSCVDMKLSLEVSEQILHMMSQSSHLEELVLETCSLRGDFVRRLAQALAGHSSSGLRELSLAGNLLDDRGMTALSRHLERCPGALRRLSLAQTGLTPRGMRALGRALATNAAFDSTLTHLDLSGNPGALGASEDSGGLYSFLSRPNVLSFLNLAGTDTALDTLFAAVSRGCCTSLTHLDASRNVFSRTKSRAAPAALQLFLSRARTLRHLGLAGCKLPPDALRALLDGLALNTHLRDLHLDLSACELRSAGAQVIQDLVCDAGAVSSLDLADNGFGSDMVTLVLAIGRSRSLRHVALGRNFNVRCKETLDDVLHRIVQLMQDDDCPLQSLSVAESRLKLGASVLLRALATNPNLTALDISGNAMGDAGAKLLAKALRVNSRLRSVVWDRNHTSALGLLDVAQALEQNHSLKAMPLPLNDVAQAQRSRPELTARAVHQIQACLLRNNRADPASSDHTTRLQPLGLVSDPSEQEVNELCQSVQEHVELLGCGAGPQGEAAVRQAEDAIQNANFSLSILPILYEAGSSPSHHWQLGQKLEGLLRQVGEVCRQDIQDFTQATLDTARSLCPQMLQGSSWREQLEGVLAGSRGLPELLPEQLLQDAFTRLRDMRLSITGTLAESIVAQALAGLSAARDQLVESLAQQATVTMPPALPAPDGGEPSLLEPGELEGLFFPEEKEEEKEKDDSPPQKWPELSHGLHLVPFIHSAAEEAEPEPELAAPGEDAEPQAGPSARGSPSPAAPGPPAGPLPRMDLPLAGQPLRHPTRARPRPRRQHHHRPPPGGPQVPPALPQEGNGLSARVDEGVEEFFSKRLIQQDRLWAPEEDPATEGGATPVPRTLRKKLGTLFAFKKPRSTRGPRTDLETSPGAAPRTRKTTFGDLLRPPTRPSRGEELGGAEGDTSSPDPAGRSRPRYTRDSKAYSMILLPAEEEATLGARPDKRRPLERGETELAPSFEQRVQVMLQRIGVSRGSGGAEGKRKQSKDGEIKKAGSDGDIMDSSTEAPPISIKSRTHSVSADPSCRPGPGSQGPESATWKTLGQQLNAELRSRGWGQQDGPGPPSPGQSPSPCRTSPSPDSLGLPEDPCLGPRNEERPLRLQRSPVLKRRPKLEAPPSPSLGSGLGTEPLPPQPTEPSSPERSPPSPATDQRGGGPNP

Target ORF information:

RefSeq Version	NM_001317026.2
Organism	Homo sapiens(human)
Definition	Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317026.2

ORF Insert Sequence:

ATGGCCCAGA CCCCCGACGG CATCTCCTGT GAGCTCCGAG GCGAGATCAC CAGGTTCCTG 
TGGCCCAAAG AGGTGGAGCT GCTGCTGAAA ACCTGGCTAC CCGGGGAGGG TGCTGTGCAA 
AACCATGTCC TGGCACTGCT ACGATGGAGA GCCTACCTGC TGCACACCAC CTGCCTCCCG 
CTGAGGGTGG ACTGCACGTT CAGCTACCTG GAGGTCCAGG CCATGGCGCT GCAGGAGACA 
CCCCCTCAGG TCACCTTTGA GCTGGAGTCC CTGCGTGAGC TGGTCCTGGA GTTTCCTGGT 
GTGGCCGCCC TGGAACAGCT GGCCCAGCAC GTGGCTGCAG CCATCAAGAA GGTCTTCCCT 
CGCTCGACCC TTGGGAAGCT ATTCCGGAGG CCCACACCAG CCTCCATGCT GGCTCGGCTG 
GAGAGAAGCA GCCCCTCGGA GTCCACTGAC CCCTGCAGCC CCTGTGGTGG CTTCTTGGAG 
ACATACGAGG CTCTGTGTGA CTACAATGGC TTCCCTTTCC GAGAGGAGAT TCAGTGGGAC 
GTGGACACCA TTTACCATCG CCAGGGCTGC CGCCATTTCA GCCTGGGAGA CTTCAGCCAC 
CTCGGCAGTC GGGACCTGGC CTTGAGTGTG GCTGCCCTGT CCTACAACCT GTGGTTCCGG 
TGCCTCTCCT GTGTGGACAT GAAGCTGAGC CTTGAGGTCT CAGAACAGAT TCTGCACATG 
ATGAGTCAGT CATCACACCT GGAGGAGCTG GTGCTGGAGA CCTGCAGCCT GAGGGGAGAC 
TTTGTCCGAC GACTGGCCCA GGCGCTGGCG GGACACTCAA GCTCTGGGCT GCGGGAGCTC 
AGCCTCGCGG GGAACCTGCT GGATGACCGA GGCATGACTG CACTCAGCAG ACACCTCGAG 
CGTTGTCCAG GAGCCCTGAG GAGACTCAGC CTGGCCCAGA CAGGGTTGAC ACCGCGAGGA 
ATGAGGGCTC TGGGCCGGGC ACTGGCCACC AATGCCGCCT TCGACTCCAC CCTGACCCAC 
CTGGACCTTT CTGGGAATCC TGGGGCGCTG GGGGCCTCCG AGGACAGTGG GGGCCTCTAT 
AGCTTCCTGA GCCGTCCTAA CGTACTGTCG TTCCTGAATC TCGCAGGCAC CGACACTGCC 
CTGGACACTC TCTTCGCAGC GGTATCCCGA GGCTGCTGCA CCAGCCTTAC CCACCTCGAC 
GCTTCGAGGA ACGTCTTCTC CCGCACGAAG TCCCGCGCCG CGCCGGCCGC GCTGCAGCTC 
TTCCTCAGCC GCGCGCGGAC GCTGCGGCAC CTGGGCCTGG CGGGCTGCAA GCTGCCGCCC 
GACGCGCTCA GAGCCCTTTT GGATGGCCTC GCGCTCAACA CGCACCTCCG CGACCTGCAC 
CTGGACCTCA GCGCTTGCGA GCTGCGCTCG GCCGGCGCCC AGGTGATACA AGACTTAGTG 
TGCGACGCAG GCGCTGTGAG CTCCCTGGAT CTGGCGGATA ACGGCTTCGG CTCAGACATG 
GTGACTCTGG TGCTGGCCAT CGGGAGAAGC CGGTCCCTGA GACATGTGGC GCTTGGAAGG 
AACTTCAACG TCCGGTGCAA GGAGACCCTG GACGACGTCC TGCACCGGAT TGTCCAGCTC 
ATGCAGGACG ACGATTGTCC TCTTCAGTCT CTGTCGGTGG CTGAGTCTCG GCTGAAGCTG 
GGTGCCAGCG TCCTACTCCG GGCCCTAGCC ACCAATCCTA ACCTGACCGC GCTGGATATC 
AGCGGCAACG CCATGGGGGA CGCGGGCGCC AAGTTGCTGG CCAAGGCGCT GCGGGTCAAC 
TCGAGGCTCC GGTCTGTGGT CTGGGACCGG AACCACACAT CTGCTTTGGG TCTGCTGGAC 
GTGGCGCAGG CGCTGGAGCA GAACCACAGC CTGAAGGCCA TGCCTCTGCC ACTGAACGAC 
GTGGCCCAGG CGCAGCGCAG CCGCCCGGAA CTGACAGCAC GTGCAGTCCA TCAGATCCAA 
GCCTGTCTCT TGAGGAACAA CCGCGCAGAC CCTGCCTCTT CTGACCACAC GACCCGCCTT 
CAGCCACTTG GTCTGGTCTC AGACCCCTCA GAGCAGGAAG TGAATGAATT GTGTCAGTCG 
GTGCAGGAGC ATGTGGAGCT GCTGGGCTGT GGGGCTGGGC CCCAGGGTGA AGCCGCTGTG 
CGCCAGGCCG AGGATGCCAT CCAAAATGCC AACTTCTCTC TCAGCATTCT CCCCATTCTA 
TATGAAGCTG GAAGCTCCCC AAGCCATCAC TGGCAGCTTG GGCAGAAGCT GGAGGGCCTT 
CTGAGACAGG TGGGCGAGGT CTGCCGCCAG GACATCCAGG ACTTCACTCA GGCCACACTG 
GACACAGCAA GGAGCCTCTG CCCACAGATG CTGCAGGGAT CCAGCTGGAG GGAGCAGCTA 
GAGGGGGTCC TGGCAGGCTC GAGGGGCCTC CCGGAGCTGC TCCCAGAGCA GCTGCTGCAA 
GATGCCTTCA CTAGGCTCAG GGACATGCGG CTATCAATCA CGGGGACCTT GGCAGAGAGC 
ATTGTGGCTC AGGCTTTGGC AGGCCTGAGT GCAGCCCGGG ATCAGCTGGT GGAGAGTCTG 
GCTCAGCAGG CAACAGTGAC AATGCCCCCT GCCCTACCAG CACCGGATGG AGGTGAGCCC 
AGCCTCCTTG AGCCTGGGGA ATTGGAAGGT CTTTTCTTCC CCGAGGAGAA GGAAGAGGAG 
AAGGAGAAGG ATGACAGTCC TCCACAGAAA TGGCCTGAGC TCAGCCACGG TCTTCACCTG 
GTCCCCTTCA TTCACAGTGC TGCTGAGGAA GCGGAGCCGG AGCCCGAGCT GGCGGCTCCG 
GGAGAAGATG CAGAGCCGCA GGCGGGGCCG TCCGCGCGCG GCTCTCCGAG CCCTGCCGCC 
CCTGGGCCCC CGGCCGGCCC GCTGCCCCGC ATGGACCTGC CACTGGCGGG GCAGCCCCTG 
CGCCATCCGA CCCGGGCCCG GCCGCGGCCG CGCCGCCAGC ACCACCACCG CCCGCCGCCG 
GGGGGCCCCC AGGTACCCCC AGCCTTGCCG CAGGAAGGGA ATGGGCTCAG TGCCCGCGTG 
GACGAGGGCG TGGAGGAATT CTTCTCCAAA AGGCTGATCC AGCAGGATCG CCTCTGGGCC 
CCCGAGGAGG ACCCGGCCAC TGAGGGGGGC GCCACTCCTG TCCCCCGTAC ACTGCGAAAG 
AAGCTGGGCA CCCTCTTTGC CTTCAAGAAG CCTCGTTCAA CGCGGGGTCC ACGGACTGAT 
CTAGAGACCA GCCCTGGGGC AGCTCCCCGA ACCCGAAAAA CTACATTTGG CGACCTACTG 
CGGCCGCCAA CCCGTCCCAG CCGTGGTGAG GAGCTTGGTG GGGCTGAGGG GGACACCAGC 
AGCCCTGACC CTGCCGGCAG GAGCCGACCT CGCTACACAA GAGATAGCAA GGCCTACTCG 
ATGATACTGC TGCCTGCCGA GGAGGAGGCA ACGCTGGGTG CCAGACCCGA CAAGCGGCGG 
CCCCTGGAGC GGGGAGAAAC AGAACTGGCT CCATCCTTTG AACAGCGGGT ACAAGTAATG 
CTGCAGAGGA TAGGCGTCAG CCGAGGCAGC GGGGGTGCCG AAGGCAAGAG GAAGCAAAGC 
AAAGATGGCG AGATCAAGAA AGCTGGCTCA GATGGTGACA TTATGGACAG TTCCACGGAG 
GCCCCTCCCA TCTCGATCAA GTCCCGCACC CACTCTGTGT CTGCTGACCC TTCCTGCAGA 
CCTGGCCCAG GGAGCCAGGG GCCTGAGTCT GCCACCTGGA AGACACTGGG GCAGCAGTTG 
AATGCGGAGC TCAGGAGCCG TGGTTGGGGC CAACAGGATG GTCCAGGCCC TCCCTCCCCT 
GGTCAAAGCC CAAGTCCCTG CAGAACCAGC CCCTCCCCAG ACAGCCTGGG CCTCCCAGAG 
GACCCTTGCT TGGGCCCCAG AAATGAAGAA CGGCCCCTGA GGCTGCAGCG CTCCCCCGTC 
CTCAAACGCA GGCCAAAACT CGAGGCACCT CCATCCCCAA GCCTAGGATC TGGCCTTGGA 
ACCGAGCCTC TGCCCCCACA GCCCACAGAG CCCTCCAGCC CTGAGCGGAG CCCACCCTCC 
CCAGCCACAG ACCAAAGAGG CGGCGGCCCC AATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CARMIL2 ( XM_011522874.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521176.1 Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu76205
Clone ID Related Accession (Same CDS sequence)	XM_011522874.1
Accession Version	XM_011522874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	capping protein, Arp2/3 and myosin-I linker protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA CCCCCGACGG CATCTCCTGT GAGCTCCGAG GCGAGATCAC CAGGTTCCTG 
TGGCCCAAAG AGGTGGAGCT GCTGCTGAAA ACCTGGCTAC CCGGGGAGGG TGCTGTGCAA 
AACCATGTCC TGGCACTGCT ACGATGGAGA GCCTACCTGC TGCACACCAC CTGCCTCCCG 
CTGAGGGTGG ACTGCACGTT CAGCTACCTG GAGGTCCAGG CCATGGCGCT GCAGGAGACA 
CCCCCTCAGG TCACCTTTGA GCTGGAGTCC CTGCGTGAGC TGGTCCTGGA GTTTCCTGGT 
GTGGCCGCCC TGGAACAGCT GGCCCAGCAC GTGGCTGCAG CCATCAAGAA GGTCTTCCCT 
CGCTCGACCC TTGGGAAGCT ATTCCGGAGG CCCACACCAG CCTCCATGCT GGCTCGGCTG 
GAGAGAAGCA GCCCCTCGGA GTCCACTGAC CCCTGCAGCC CCTGTGGTGG CTTCTTGGAG 
ACATACGAGG CTCTGTGTGA CTACAATGGC TTCCCTTTCC GAGAGGAGAT TCAGTGGGAC 
GTGGACACCA TTTACCATCG CCAGGGCTGC CGCCATTTCA GCCTGGGAGA CTTCAGCCAC 
CTCGGCAGTC GGGACCTGGC CTTGAGTGTG GCTGCCCTGT CCTACAACCT GTGGTTCCGG 
TGCCTCTCCT GTGTGGACAT GAAGCTGGTG AGGGGGTTCG GGAGCCTTGA GGTCTCAGAA 
CAGATTCTGC ACATGATGAG TCAGTCATCA CACCTGGAGG AGCTGGTGCT GGAGACCTGC 
AGCCTGAGGG GAGACTTTGT CCGACGACTG GCCCAGGCGC TGGCGGGACA CTCAAGCTCT 
GGGCTGCGGG AGCTCAGCCT CGCGGGGAAC CTGCTGGATG ACCGAGGCAT GACTGCACTC 
AGCAGACACC TCGAGCGTTG TCCAGGAGCC CTGAGGAGAC TCAGCCTGGC CCAGACAGGG 
TTGACACCGC GAGGAATGAG GGCTCTGGGC CGGGCACTGG CCACCAATGC CGCCTTCGAC 
TCCACCCTGA CCCACCTGGA CCTTTCTGGG AATCCTGGGG CGCTGGGGGC CTCCGAGGAC 
AGTGGGGGCC TCTATAGCTT CCTGAGCCGT CCTAACGTAC TGTCGTTCCT GAATCTCGCA 
GGCACCGACA CTGCCCTGGA CACTCTCTTC GCAGCGGTAT CCCGAGGCTG CTGCACCAGC 
CTTACCCACC TCGACGCTTC GAGGAACGTC TTCTCCCGCA CGAAGTCCCG CGCCGCGCCG 
GCCGCGCTGC AGCTCTTCCT CAGCCGCGCG CGGACGCTGC GGCACCTGGG CCTGGCGGGC 
TGCAAGCTGC CGCCCGACGC GCTCAGAGCC CTTTTGGATG GCCTCGCGCT CAACACGCAC 
CTCCGCGACC TGCACCTGGA CCTCAGCGCT TGCGAGCTGC GCTCGGCCGG CGCCCAGGTG 
ATACAAGACT TAGTGTGCGA CGCAGGCGCT GTGAGCTCCC TGGATCTGGC GGATAACGGC 
TTCGGCTCAG ACATGGTGAC TCTGGTGCTG GCCATCGGGA GAAGCCGGTC CCTGAGACAT 
GTGGCGCTTG GAAGGAACTT CAACGTCCGG TGCAAGGAGA CCCTGGACGA CGTCCTGCAC 
CGGATTGTCC AGCTCATGCA GGACGACGAT TGTCCTCTTC AGTCTCTGTC GGTGGCTGAG 
TCTCGGCTGA AGCTGGGTGC CAGCGTCCTA CTCCGGGCCC TAGCCACCAA TCCTAACCTG 
ACCGCGCTGG ATATCAGCGG CAACGCCATG GGGGACGCGG GCGCCAAGTT GCTGGCCAAG 
GCGCTGCGGG TCAACTCGAG GCTCCGGTCT GTGGTCTGGG ACCGGAACCA CACATCTGCT 
TTGGGTCTGC TGGACGTGGC GCAGGCGCTG GAGCAGAACC ACAGCCTGAA GGCCATGCCT 
CTGCCACTGA ACGACGTGGC CCAGGCGCAG CGCAGCCGCC CGGAACTGAC AGCACGTGCA 
GTCCATCAGA TCCAAGCCTG TCTCTTGAGG AACAACCGCG CAGACCCTGC CTCTTCTGAC 
CACACGACCC GCCTTCAGCC ACTTGGTCTG GTCTCAGACC CCTCAGAGCA GGAAGTGAAT 
GAATTGTGTC AGTCGGTGCA GGAGCATGTG GAGCTGCTGG GCTGTGGGGC TGGGCCCCAG 
GGTGAAGCCG CTGTGCGCCA GGCCGAGGAT GCCATCCAAA ATGCCAACTT CTCTCTCAGC 
ATTCTCCCCA TTCTATATGA AGCTGGAAGC TCCCCAAGCC ATCACTGGCA GCTTGGGCAG 
AAGCTGGAGG GCCTTCTGAG ACAGGTGGGC GAGGTCTGCC GCCAGGACAT CCAGGACTTC 
ACTCAGGCCA CACTGGACAC AGCAAGGAGC CTCTGCCCAC AGATGCTGCA GGGATCCAGC 
TGGAGGGAGC AGCTAGAGGG GGTCCTGGCA GGCTCGAGGG GCCTCCCGGA GCTGCTCCCA 
GAGCAGCTGC TGCAAGATGC CTTCACTAGG CTCAGGGACA TGCGGCTATC AATCACGGGG 
ACCTTGGCAG AGAGCATTGT GGCTCAGGCT TTGGCAGGCC TGAGTGCAGC CCGGGATCAG 
CTGGTGGAGA GTCTGGCTCA GCAGGCAACA GTGACAATGC CCCCTGCCCT ACCAGCACCG 
GATGGAGGTG AGCCCAGCCT CCTTGAGCCT GGGGAATTGG AAGGTCTTTT CTTCCCCGAG 
GAGAAGGAAG AGGAGAAGGA GAAGGATGAC AGTCCTCCAC AGAAATGGCC TGAGCTCAGC 
CACGGTCTTC ACCTGGTCCC CTTCATTCAC AGTGCTGCTG AGGAAGCGGA GCCGGAGCCC 
GAGCTGGCGG CTCCGGGAGA AGATGCAGAG CCGCAGGCGG GGCCGTCCGC GCGCGGCTCT 
CCGAGCCCTG CCGCCCCTGG GCCCCCGGCC GGCCCGCTGC CCCGCATGGA CCTGCCACTG 
GCGGGGCAGC CCCTGCGCCA TCCGACCCGG GCCCGGCCGC GGCCGCGCCG CCAGCACCAC 
CACCGCCCGC CGCCGGGGGG CCCCCAGGTA CCCCCAGCCT TGCCGCAGGA AGGGAATGGG 
CTCAGTGCCC GCGTGGACGA GGGCGTGGAG GAATTCTTCT CCAAAAGGCT GATCCAGCAG 
GATCGCCTCT GGGCCCCCGA GGAGGACCCG GCCACTGAGG GGGGCGCCAC TCCTGTCCCC 
CGTACACTGC GAAAGAAGCT GGGCACCCTC TTTGCCTTCA AGAAGCCTCG TTCAACGCGG 
GGTCCACGGA CTGATCTAGA GACCAGCCCT GGGGCAGCTC CCCGAACCCG AAAAACTACA 
TTTGGCGACC TACTGCGGCC GCCAACCCGT CCCAGCCGTG GTGAGGAGCT TGGTGGGGCT 
GAGGGGGACA CCAGCAGCCC TGACCCTGCC GGCAGGAGCC GACCTCGCTA CACAAGAGAT 
AGCAAGGCCT ACTCGATGAT ACTGCTGCCT GCCGAGGAGG AGGCAACGCT GGGTGCCAGA 
CCCGACAAGC GGCGGCCCCT GGAGCGGGGA GAAACAGAAC TGGCTCCATC CTTTGAACAG 
CGGGTACAAG TAATGCTGCA GAGGATAGGC GTCAGCCGAG GCAGCGGGGG TGCCGAAGGC 
AAGAGGAAGC AAAGCAAAGA TGGCGAGATC AAGAAAGCTG GCTCAGATGG TGACATTATG 
GACAGTTCCA CGGAGGCCCC TCCCATCTCG ATCAAGTCCC GCACCCACTC TGTGTCTGCT 
GACCCTTCCT GCAGACCTGG CCCAGGGAGC CAGGGGCCTG AGTCTGCCAC CTGGAAGACA 
CTGGGGCAGC AGTTGAATGC GGAGCTCAGG AGCCGTGGTT GGGGCCAACA GGATGGTCCA 
GGCCCTCCCT CCCCTGGTCA AAGCCCAAGT CCCTGCAGAA CCAGCCCCTC CCCAGACAGC 
CTGGGCCTCC CAGAGGACCC TTGCTTGGGC CCCAGAAATG AAGAACGGCC CCTGAGGCTG 
CAGCGCTCCC CCGTCCTCAA ACGCAGGCCA AAACTCGAGG CACCTCCATC CCCAAGCCTA 
GGATCTGGCC TTGGAACCGA GCCTCTGCCC CCACAGCCCA CAGAGCCCTC CAGCCCTGAG 
CGGAGCCCAC CCTCCCCAGC CACAGACCAA AGAGGCGGCG GCCCCAATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521176.1
CDS	244..4377
Translation	MAQTPDGISCELRGEITRFLWPKEVELLLKTWLPGEGAVQNHVLALLRWRAYLLHTTCLPLRVDCTFSYLEVQAMALQETPPQVTFELESLRELVLEFPGVAALEQLAQHVAAAIKKVFPRSTLGKLFRRPTPASMLARLERSSPSESTDPCSPCGGFLETYEALCDYNGFPFREEIQWDVDTIYHRQGCRHFSLGDFSHLGSRDLALSVAALSYNLWFRCLSCVDMKLVRGFGSLEVSEQILHMMSQSSHLEELVLETCSLRGDFVRRLAQALAGHSSSGLRELSLAGNLLDDRGMTALSRHLERCPGALRRLSLAQTGLTPRGMRALGRALATNAAFDSTLTHLDLSGNPGALGASEDSGGLYSFLSRPNVLSFLNLAGTDTALDTLFAAVSRGCCTSLTHLDASRNVFSRTKSRAAPAALQLFLSRARTLRHLGLAGCKLPPDALRALLDGLALNTHLRDLHLDLSACELRSAGAQVIQDLVCDAGAVSSLDLADNGFGSDMVTLVLAIGRSRSLRHVALGRNFNVRCKETLDDVLHRIVQLMQDDDCPLQSLSVAESRLKLGASVLLRALATNPNLTALDISGNAMGDAGAKLLAKALRVNSRLRSVVWDRNHTSALGLLDVAQALEQNHSLKAMPLPLNDVAQAQRSRPELTARAVHQIQACLLRNNRADPASSDHTTRLQPLGLVSDPSEQEVNELCQSVQEHVELLGCGAGPQGEAAVRQAEDAIQNANFSLSILPILYEAGSSPSHHWQLGQKLEGLLRQVGEVCRQDIQDFTQATLDTARSLCPQMLQGSSWREQLEGVLAGSRGLPELLPEQLLQDAFTRLRDMRLSITGTLAESIVAQALAGLSAARDQLVESLAQQATVTMPPALPAPDGGEPSLLEPGELEGLFFPEEKEEEKEKDDSPPQKWPELSHGLHLVPFIHSAAEEAEPEPELAAPGEDAEPQAGPSARGSPSPAAPGPPAGPLPRMDLPLAGQPLRHPTRARPRPRRQHHHRPPPGGPQVPPALPQEGNGLSARVDEGVEEFFSKRLIQQDRLWAPEEDPATEGGATPVPRTLRKKLGTLFAFKKPRSTRGPRTDLETSPGAAPRTRKTTFGDLLRPPTRPSRGEELGGAEGDTSSPDPAGRSRPRYTRDSKAYSMILLPAEEEATLGARPDKRRPLERGETELAPSFEQRVQVMLQRIGVSRGSGGAEGKRKQSKDGEIKKAGSDGDIMDSSTEAPPISIKSRTHSVSADPSCRPGPGSQGPESATWKTLGQQLNAELRSRGWGQQDGPGPPSPGQSPSPCRTSPSPDSLGLPEDPCLGPRNEERPLRLQRSPVLKRRPKLEAPPSPSLGSGLGTEPLPPQPTEPSSPERSPPSPATDQRGGGPNP

Target ORF information:

RefSeq Version	XM_011522874.1
Organism	Homo sapiens(human)
Definition	Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522874.1

ORF Insert Sequence:

ATGGCCCAGA CCCCCGACGG CATCTCCTGT GAGCTCCGAG GCGAGATCAC CAGGTTCCTG 
TGGCCCAAAG AGGTGGAGCT GCTGCTGAAA ACCTGGCTAC CCGGGGAGGG TGCTGTGCAA 
AACCATGTCC TGGCACTGCT ACGATGGAGA GCCTACCTGC TGCACACCAC CTGCCTCCCG 
CTGAGGGTGG ACTGCACGTT CAGCTACCTG GAGGTCCAGG CCATGGCGCT GCAGGAGACA 
CCCCCTCAGG TCACCTTTGA GCTGGAGTCC CTGCGTGAGC TGGTCCTGGA GTTTCCTGGT 
GTGGCCGCCC TGGAACAGCT GGCCCAGCAC GTGGCTGCAG CCATCAAGAA GGTCTTCCCT 
CGCTCGACCC TTGGGAAGCT ATTCCGGAGG CCCACACCAG CCTCCATGCT GGCTCGGCTG 
GAGAGAAGCA GCCCCTCGGA GTCCACTGAC CCCTGCAGCC CCTGTGGTGG CTTCTTGGAG 
ACATACGAGG CTCTGTGTGA CTACAATGGC TTCCCTTTCC GAGAGGAGAT TCAGTGGGAC 
GTGGACACCA TTTACCATCG CCAGGGCTGC CGCCATTTCA GCCTGGGAGA CTTCAGCCAC 
CTCGGCAGTC GGGACCTGGC CTTGAGTGTG GCTGCCCTGT CCTACAACCT GTGGTTCCGG 
TGCCTCTCCT GTGTGGACAT GAAGCTGGTG AGGGGGTTCG GGAGCCTTGA GGTCTCAGAA 
CAGATTCTGC ACATGATGAG TCAGTCATCA CACCTGGAGG AGCTGGTGCT GGAGACCTGC 
AGCCTGAGGG GAGACTTTGT CCGACGACTG GCCCAGGCGC TGGCGGGACA CTCAAGCTCT 
GGGCTGCGGG AGCTCAGCCT CGCGGGGAAC CTGCTGGATG ACCGAGGCAT GACTGCACTC 
AGCAGACACC TCGAGCGTTG TCCAGGAGCC CTGAGGAGAC TCAGCCTGGC CCAGACAGGG 
TTGACACCGC GAGGAATGAG GGCTCTGGGC CGGGCACTGG CCACCAATGC CGCCTTCGAC 
TCCACCCTGA CCCACCTGGA CCTTTCTGGG AATCCTGGGG CGCTGGGGGC CTCCGAGGAC 
AGTGGGGGCC TCTATAGCTT CCTGAGCCGT CCTAACGTAC TGTCGTTCCT GAATCTCGCA 
GGCACCGACA CTGCCCTGGA CACTCTCTTC GCAGCGGTAT CCCGAGGCTG CTGCACCAGC 
CTTACCCACC TCGACGCTTC GAGGAACGTC TTCTCCCGCA CGAAGTCCCG CGCCGCGCCG 
GCCGCGCTGC AGCTCTTCCT CAGCCGCGCG CGGACGCTGC GGCACCTGGG CCTGGCGGGC 
TGCAAGCTGC CGCCCGACGC GCTCAGAGCC CTTTTGGATG GCCTCGCGCT CAACACGCAC 
CTCCGCGACC TGCACCTGGA CCTCAGCGCT TGCGAGCTGC GCTCGGCCGG CGCCCAGGTG 
ATACAAGACT TAGTGTGCGA CGCAGGCGCT GTGAGCTCCC TGGATCTGGC GGATAACGGC 
TTCGGCTCAG ACATGGTGAC TCTGGTGCTG GCCATCGGGA GAAGCCGGTC CCTGAGACAT 
GTGGCGCTTG GAAGGAACTT CAACGTCCGG TGCAAGGAGA CCCTGGACGA CGTCCTGCAC 
CGGATTGTCC AGCTCATGCA GGACGACGAT TGTCCTCTTC AGTCTCTGTC GGTGGCTGAG 
TCTCGGCTGA AGCTGGGTGC CAGCGTCCTA CTCCGGGCCC TAGCCACCAA TCCTAACCTG 
ACCGCGCTGG ATATCAGCGG CAACGCCATG GGGGACGCGG GCGCCAAGTT GCTGGCCAAG 
GCGCTGCGGG TCAACTCGAG GCTCCGGTCT GTGGTCTGGG ACCGGAACCA CACATCTGCT 
TTGGGTCTGC TGGACGTGGC GCAGGCGCTG GAGCAGAACC ACAGCCTGAA GGCCATGCCT 
CTGCCACTGA ACGACGTGGC CCAGGCGCAG CGCAGCCGCC CGGAACTGAC AGCACGTGCA 
GTCCATCAGA TCCAAGCCTG TCTCTTGAGG AACAACCGCG CAGACCCTGC CTCTTCTGAC 
CACACGACCC GCCTTCAGCC ACTTGGTCTG GTCTCAGACC CCTCAGAGCA GGAAGTGAAT 
GAATTGTGTC AGTCGGTGCA GGAGCATGTG GAGCTGCTGG GCTGTGGGGC TGGGCCCCAG 
GGTGAAGCCG CTGTGCGCCA GGCCGAGGAT GCCATCCAAA ATGCCAACTT CTCTCTCAGC 
ATTCTCCCCA TTCTATATGA AGCTGGAAGC TCCCCAAGCC ATCACTGGCA GCTTGGGCAG 
AAGCTGGAGG GCCTTCTGAG ACAGGTGGGC GAGGTCTGCC GCCAGGACAT CCAGGACTTC 
ACTCAGGCCA CACTGGACAC AGCAAGGAGC CTCTGCCCAC AGATGCTGCA GGGATCCAGC 
TGGAGGGAGC AGCTAGAGGG GGTCCTGGCA GGCTCGAGGG GCCTCCCGGA GCTGCTCCCA 
GAGCAGCTGC TGCAAGATGC CTTCACTAGG CTCAGGGACA TGCGGCTATC AATCACGGGG 
ACCTTGGCAG AGAGCATTGT GGCTCAGGCT TTGGCAGGCC TGAGTGCAGC CCGGGATCAG 
CTGGTGGAGA GTCTGGCTCA GCAGGCAACA GTGACAATGC CCCCTGCCCT ACCAGCACCG 
GATGGAGGTG AGCCCAGCCT CCTTGAGCCT GGGGAATTGG AAGGTCTTTT CTTCCCCGAG 
GAGAAGGAAG AGGAGAAGGA GAAGGATGAC AGTCCTCCAC AGAAATGGCC TGAGCTCAGC 
CACGGTCTTC ACCTGGTCCC CTTCATTCAC AGTGCTGCTG AGGAAGCGGA GCCGGAGCCC 
GAGCTGGCGG CTCCGGGAGA AGATGCAGAG CCGCAGGCGG GGCCGTCCGC GCGCGGCTCT 
CCGAGCCCTG CCGCCCCTGG GCCCCCGGCC GGCCCGCTGC CCCGCATGGA CCTGCCACTG 
GCGGGGCAGC CCCTGCGCCA TCCGACCCGG GCCCGGCCGC GGCCGCGCCG CCAGCACCAC 
CACCGCCCGC CGCCGGGGGG CCCCCAGGTA CCCCCAGCCT TGCCGCAGGA AGGGAATGGG 
CTCAGTGCCC GCGTGGACGA GGGCGTGGAG GAATTCTTCT CCAAAAGGCT GATCCAGCAG 
GATCGCCTCT GGGCCCCCGA GGAGGACCCG GCCACTGAGG GGGGCGCCAC TCCTGTCCCC 
CGTACACTGC GAAAGAAGCT GGGCACCCTC TTTGCCTTCA AGAAGCCTCG TTCAACGCGG 
GGTCCACGGA CTGATCTAGA GACCAGCCCT GGGGCAGCTC CCCGAACCCG AAAAACTACA 
TTTGGCGACC TACTGCGGCC GCCAACCCGT CCCAGCCGTG GTGAGGAGCT TGGTGGGGCT 
GAGGGGGACA CCAGCAGCCC TGACCCTGCC GGCAGGAGCC GACCTCGCTA CACAAGAGAT 
AGCAAGGCCT ACTCGATGAT ACTGCTGCCT GCCGAGGAGG AGGCAACGCT GGGTGCCAGA 
CCCGACAAGC GGCGGCCCCT GGAGCGGGGA GAAACAGAAC TGGCTCCATC CTTTGAACAG 
CGGGTACAAG TAATGCTGCA GAGGATAGGC GTCAGCCGAG GCAGCGGGGG TGCCGAAGGC 
AAGAGGAAGC AAAGCAAAGA TGGCGAGATC AAGAAAGCTG GCTCAGATGG TGACATTATG 
GACAGTTCCA CGGAGGCCCC TCCCATCTCG ATCAAGTCCC GCACCCACTC TGTGTCTGCT 
GACCCTTCCT GCAGACCTGG CCCAGGGAGC CAGGGGCCTG AGTCTGCCAC CTGGAAGACA 
CTGGGGCAGC AGTTGAATGC GGAGCTCAGG AGCCGTGGTT GGGGCCAACA GGATGGTCCA 
GGCCCTCCCT CCCCTGGTCA AAGCCCAAGT CCCTGCAGAA CCAGCCCCTC CCCAGACAGC 
CTGGGCCTCC CAGAGGACCC TTGCTTGGGC CCCAGAAATG AAGAACGGCC CCTGAGGCTG 
CAGCGCTCCC CCGTCCTCAA ACGCAGGCCA AAACTCGAGG CACCTCCATC CCCAAGCCTA 
GGATCTGGCC TTGGAACCGA GCCTCTGCCC CCACAGCCCA CAGAGCCCTC CAGCCCTGAG 
CGGAGCCCAC CCTCCCCAGC CACAGACCAA AGAGGCGGCG GCCCCAATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CARMIL2 ( XM_011522875.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521177.2 Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu85864
Clone ID Related Accession (Same CDS sequence)	XM_011522875.2
Accession Version	XM_011522875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	capping protein, Arp2/3 and myosin-I linker protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522875.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA CCCCCGACGG CATCTCCTGT GAGCTCCGAG GCGAGATCAC CAGGTTCCTG 
TGGCCCAAAG AGGTGGAGCT GCTGCTGAAA ACCTGGCTAC CCGGGGAGGG TGCTGTGCAA 
AACCATGTCC TGGCACTGCT ACGATGGAGA GCCTACCTGC TGCACACCAC CTGCCTCCCG 
CTGAGGGTGG ACTGCACGTT CAGCTACCTG GAGGTCCAGG CCATGGCGCT GCAGGAGACA 
CCCCCTCAGG TCACCTTTGA GCTGGAGTCC CTGCGTGAGC TGGTCCTGGA GTTTCCTGGT 
GTGGCCGCCC TGGAACAGCT GGCCCAGCAC GTGGCTGCAG CCATCAAGAA GGTCTTCCCT 
CGCTCGACCC TTGGGAAGCT ATTCCGGAGG CCCACACCAG CCTCCATGCT GGCTCGGCTG 
GAGAGAAGCA GCCCCTCGGA GTCCACTGAC CCCTGCAGCC CCTGTGGTGG CTTCTTGGAG 
ACATACGAGG CTCTGTGTGA CTACAATGGC TTCCCTTTCC GAGAGGAGAT TCAGTGGGAC 
GTGGACACCA TTTACCATCG CCAGGGCTGC CGCCATTTCA GCCTGGGAGA CTTCAGCCAC 
CTCGGCAGTC GGGACCTGGC CTTGAGTGTG GCTGCCCTGT CCTACAACCT GTGGTTCCGG 
TGCCTCTCCT GTGTGGACAT GAAGCTGGTG AGGGGGTTCG GGAGCCTTGA GGTCTCAGAA 
CAGATTCTGC ACATGATGAG TCAGTCATCA CACCTGGAGG AGCTGGTGCT GGAGACCTGC 
AGCCTGAGGG GAGACTTTGT CCGACGACTG GCCCAGGCGC TGGCGGGACA CTCAAGCTCT 
GGGCTGCGGG AGCTCAGCCT CGCGGGGAAC CTGCTGGATG ACCGAGGCAT GACTGCACTC 
AGCAGACACC TCGAGCGTTG TCCAGGAGCC CTGAGGAGAC TCAGCCTGGC CCAGACAGGG 
TTGACACCGC GAGGAATGAG GGCTCTGGGC CGGGCACTGG CCACCAATGC CGCCTTCGAC 
TCCACCCTGA CCCACCTGGA CCTTTCTGGG AATCCTGGGG CGCTGGGGGC CTCCGAGGAC 
AGTGGGGGCC TCTATAGCTT CCTGAGCCGT CCTAACGTAC TGTCGTTCCT GAATCTCGCA 
GGCACCGACA CTGCCCTGGA CACTCTCTTC GCAGCGGTAT CCCGAGGCTG CTGCACCAGC 
CTTACCCACC TCGACGCTTC GAGGAACGTC TTCTCCCGCA CGAAGTCCCG CGCCGCGCCG 
GCCGCGCTGC AGCTCTTCCT CAGCCGCGCG CGGACGCTGC GGCACCTGGG CCTGGCGGGC 
TGCAAGCTGC CGCCCGACGC GCTCAGAGCC CTTTTGGATG GCCTCGCGCT CAACACGCAC 
CTCCGCGACC TGCACCTGGA CCTCAGCGCT TGCGAGCTGC GCTCGGCCGG CGCCCAGGTG 
ATACAAGACT TAGTGTGCGA CGCAGGCGCT GTGAGCTCCC TGGATCTGGC GGATAACGGC 
TTCGGCTCAG ACATGGTGAC TCTGGTGCTG GCCATCGGGA GAAGCCGGTC CCTGAGACAT 
GTGGCGCTTG GAAGGAACTT CAACGTCCGG TGCAAGGAGA CCCTGGACGA CGTCCTGCAC 
CGGATTGTCC AGCTCATGCA GGACGACGAT TGTCCTCTTC AGTCTCTGTC GGTGGCTGAG 
TCTCGGCTGA AGCTGGGTGC CAGCGTCCTA CTCCGGGCCC TAGCCACCAA TCCTAACCTG 
ACCGCGCTGG ATATCAGCGG CAACGCCATG GGGGACGCGG GCGCCAAGTT GCTGGCCAAG 
GCGCTGCGGG TCAACTCGAG GCTCCGGTCT GTGGTCTGGG ACCGGAACCA CACATCTGCT 
TTGGGTCTGC TGGACGTGGC GCAGGCGCTG GAGCAGAACC ACAGCCTGAA GGCCATGCCT 
CTGCCACTGA ACGACGTGGC CCAGGCGCAG CGCAGCCGCC CGGAACTGAC AGCACGTGCA 
GTCCATCAGA TCCAAGCCTG TCTCTTGAGG AACAACCGCG CAGACCCTGC CTCTTCTGAC 
CACACGACCC GCCTTCAGCC ACTTGGTCTG GTCTCAGACC CCTCAGAGCA GGAAGTGAAT 
GAATTGTGTC AGTCGGTGCA GGAGCATGTG GAGCTGCTGG GCTGTGGGGC TGGGCCCCAG 
GGTGAAGCCG CTGTGCGCCA GGCCGAGGAT GCCATCCAAA ATGCCAACTT CTCTCTCAGC 
ATTCTCCCCA TTCTATATGA AGCTGGAAGC TCCCCAAGCC ATCACTGGCA GCTTGGGCAG 
AAGCTGGAGG GCCTTCTGAG ACAGGTGGGC GAGGTCTGCC GCCAGGACAT CCAGGACTTC 
ACTCAGGCCA CACTGGACAC AGCAAGGAGC CTCTGCCCAC AGATGCTGCA GGGATCCAGC 
TGGAGGGAGC AGCTAGAGGG GGTCCTGGCA GGCTCGAGGG GCCTCCCGGA GCTGCTCCCA 
GAGCAGCTGC TGCAAGATGC CTTCACTAGG CTCAGGGACA TGCGGCTATC AATCACGGGG 
ACCTTGGCAG AGAGCATTGT GGCTCAGGCT TTGGCAGGCC TGAGTGCAGC CCGGGATCAG 
CTGGTGGAGA GTCTGGCTCA GCAGGCAACA GTGACAATGC CCCCTGCCCT ACCAGCACCG 
GATGGAGGTG AGCCCAGCCT CCTTGAGCCT GGGGAATTGG AAGGTCTTTT CTTCCCCGAG 
GAGAAGGAAG AGGAGAAGGA GAAGGATGAC AGTCCTCCAC AGAAATGGCC TGAGCTCAGC 
CACGGTCTTC ACCTGGTCCC CTTCATTCAC AGTGCTGCTG AGGAAGCGGA GCCGGAGCCC 
GAGCTGGCGG CTCCGGGAGA AGATGCAGAG CCGCAGGCGG GGCCGTCCGC GCGCGGCTCT 
CCGAGCCCTG CCGCCCCTGG GCCCCCGGCC GGCCCGCTGC CCCGCATGGA CCTGCCACTG 
GCGGGGCAGC CCCTGCGCCA TCCGACCCGG GCCCGGCCGC GGCCGCGCCG CCAGCACCAC 
CACCGCCCGC CGCCGGGGGG CCCCCAGGTA CCCCCAGCCT TGCCGCAGGA AGGGAATGGG 
CTCAGTGCCC GCGTGGACGA GGGCGTGGAG GAATTCTTCT CCAAAAGGCT GATCCAGCAG 
GATCGCCTCT GGGCCCCCGA GGAGGACCCG GCCACTGAGG GGGGCGCCAC TCCTGTCCCC 
CGTACACTGC GAAAGAAGCT GGGCACCCTC TTTGCCTTCA AGAAGCCTCG TTCAACGCGG 
GGTCCACGGA CTGATCTAGA GACCAGCCCT GGGGCAGCTC CCCGAACCCG AAAAACTACA 
TTTGGCGACC TACTGCGGCC GCCAACCCGT CCCAGCCGTG GTGAGGAGCT TGGTGGGGCT 
GAGGGGGACA CCAGCAGCCC TGACCCTGCC GGCAGGAGCC GACCTCGCTA CACAAGAGAT 
AGCAAGGCCT ACTCGATGAT ACTGCTGCCT GCCGAGGAGG AGGCAACGCT GGGTGCCAGA 
CCCGACAAGC GGCGGCCCCT GGAGCGGGGA GAAACAGAAC TGGCTCCATC CTTTGAACAG 
CGGGTACAAG TAATGCTGCA GAGGATAGGC GTCAGCCGAG GCAGCGGGGG TGCCGAAGGC 
AAGAGGAAGC AAAGCAAAGA TGGCGAGATC AAGAAAGCTG GCTCAGATGG TGACATTATG 
GACAGTTCCA CGGAGGCCCC TCCCATCTCG ATCAAGTCCC GCACCCACTC TGTGTCTGCT 
GACCCTTCCT GCAGACCTGG CCCAGGGAGC CAGGGGCCTG AGTCTGCCAC CTGGAAGACA 
CTGGGGCAGC AGTTGAATGC GGAGCTCAGG AGCCGTGGTT GGGGCCAACA GGATGGTCCA 
GGCCCTCCCT CCCCTGGTCA AAGCCCAAGT CCCTGCAGAA CCAGCCCCTC CCCAGACAGC 
CTGGGCCTCC CAGAGGACCC TTGCTTGGGC CCCAGAAATG AAGATGGCCA GCTGAGGCCG 
AGGCCTCTCT CGGCAGGGCG GCGAGCAGTG TCTGTGCATG AGGACCAGCT CCAGGCCCCT 
GCTGCCTTTC TGTGTCCCTT AGAACGGCCC CTGAGGCTGC AGCGCTCCCC CGTCCTCAAA 
CGCAGGCCAA AACTCGAGGC ACCTCCATCC CCAAGCCTAG GATCTGGCCT TGGAACCGAG 
CCTCTGCCCC CACAGCCCAC AGAGCCCTCC AGCCCTGAGC GGAGCCCACC CTCCCCAGCC 
ACAGACCAAA GAGGCGGCGG CCCCAATCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521177.2
CDS	244..4476
Translation	MAQTPDGISCELRGEITRFLWPKEVELLLKTWLPGEGAVQNHVLALLRWRAYLLHTTCLPLRVDCTFSYLEVQAMALQETPPQVTFELESLRELVLEFPGVAALEQLAQHVAAAIKKVFPRSTLGKLFRRPTPASMLARLERSSPSESTDPCSPCGGFLETYEALCDYNGFPFREEIQWDVDTIYHRQGCRHFSLGDFSHLGSRDLALSVAALSYNLWFRCLSCVDMKLVRGFGSLEVSEQILHMMSQSSHLEELVLETCSLRGDFVRRLAQALAGHSSSGLRELSLAGNLLDDRGMTALSRHLERCPGALRRLSLAQTGLTPRGMRALGRALATNAAFDSTLTHLDLSGNPGALGASEDSGGLYSFLSRPNVLSFLNLAGTDTALDTLFAAVSRGCCTSLTHLDASRNVFSRTKSRAAPAALQLFLSRARTLRHLGLAGCKLPPDALRALLDGLALNTHLRDLHLDLSACELRSAGAQVIQDLVCDAGAVSSLDLADNGFGSDMVTLVLAIGRSRSLRHVALGRNFNVRCKETLDDVLHRIVQLMQDDDCPLQSLSVAESRLKLGASVLLRALATNPNLTALDISGNAMGDAGAKLLAKALRVNSRLRSVVWDRNHTSALGLLDVAQALEQNHSLKAMPLPLNDVAQAQRSRPELTARAVHQIQACLLRNNRADPASSDHTTRLQPLGLVSDPSEQEVNELCQSVQEHVELLGCGAGPQGEAAVRQAEDAIQNANFSLSILPILYEAGSSPSHHWQLGQKLEGLLRQVGEVCRQDIQDFTQATLDTARSLCPQMLQGSSWREQLEGVLAGSRGLPELLPEQLLQDAFTRLRDMRLSITGTLAESIVAQALAGLSAARDQLVESLAQQATVTMPPALPAPDGGEPSLLEPGELEGLFFPEEKEEEKEKDDSPPQKWPELSHGLHLVPFIHSAAEEAEPEPELAAPGEDAEPQAGPSARGSPSPAAPGPPAGPLPRMDLPLAGQPLRHPTRARPRPRRQHHHRPPPGGPQVPPALPQEGNGLSARVDEGVEEFFSKRLIQQDRLWAPEEDPATEGGATPVPRTLRKKLGTLFAFKKPRSTRGPRTDLETSPGAAPRTRKTTFGDLLRPPTRPSRGEELGGAEGDTSSPDPAGRSRPRYTRDSKAYSMILLPAEEEATLGARPDKRRPLERGETELAPSFEQRVQVMLQRIGVSRGSGGAEGKRKQSKDGEIKKAGSDGDIMDSSTEAPPISIKSRTHSVSADPSCRPGPGSQGPESATWKTLGQQLNAELRSRGWGQQDGPGPPSPGQSPSPCRTSPSPDSLGLPEDPCLGPRNEDGQLRPRPLSAGRRAVSVHEDQLQAPAAFLCPLERPLRLQRSPVLKRRPKLEAPPSPSLGSGLGTEPLPPQPTEPSSPERSPPSPATDQRGGGPNP

Target ORF information:

RefSeq Version	XM_011522875.2
Organism	Homo sapiens(human)
Definition	Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522875.2

ORF Insert Sequence:

ATGGCCCAGA CCCCCGACGG CATCTCCTGT GAGCTCCGAG GCGAGATCAC CAGGTTCCTG 
TGGCCCAAAG AGGTGGAGCT GCTGCTGAAA ACCTGGCTAC CCGGGGAGGG TGCTGTGCAA 
AACCATGTCC TGGCACTGCT ACGATGGAGA GCCTACCTGC TGCACACCAC CTGCCTCCCG 
CTGAGGGTGG ACTGCACGTT CAGCTACCTG GAGGTCCAGG CCATGGCGCT GCAGGAGACA 
CCCCCTCAGG TCACCTTTGA GCTGGAGTCC CTGCGTGAGC TGGTCCTGGA GTTTCCTGGT 
GTGGCCGCCC TGGAACAGCT GGCCCAGCAC GTGGCTGCAG CCATCAAGAA GGTCTTCCCT 
CGCTCGACCC TTGGGAAGCT ATTCCGGAGG CCCACACCAG CCTCCATGCT GGCTCGGCTG 
GAGAGAAGCA GCCCCTCGGA GTCCACTGAC CCCTGCAGCC CCTGTGGTGG CTTCTTGGAG 
ACATACGAGG CTCTGTGTGA CTACAATGGC TTCCCTTTCC GAGAGGAGAT TCAGTGGGAC 
GTGGACACCA TTTACCATCG CCAGGGCTGC CGCCATTTCA GCCTGGGAGA CTTCAGCCAC 
CTCGGCAGTC GGGACCTGGC CTTGAGTGTG GCTGCCCTGT CCTACAACCT GTGGTTCCGG 
TGCCTCTCCT GTGTGGACAT GAAGCTGGTG AGGGGGTTCG GGAGCCTTGA GGTCTCAGAA 
CAGATTCTGC ACATGATGAG TCAGTCATCA CACCTGGAGG AGCTGGTGCT GGAGACCTGC 
AGCCTGAGGG GAGACTTTGT CCGACGACTG GCCCAGGCGC TGGCGGGACA CTCAAGCTCT 
GGGCTGCGGG AGCTCAGCCT CGCGGGGAAC CTGCTGGATG ACCGAGGCAT GACTGCACTC 
AGCAGACACC TCGAGCGTTG TCCAGGAGCC CTGAGGAGAC TCAGCCTGGC CCAGACAGGG 
TTGACACCGC GAGGAATGAG GGCTCTGGGC CGGGCACTGG CCACCAATGC CGCCTTCGAC 
TCCACCCTGA CCCACCTGGA CCTTTCTGGG AATCCTGGGG CGCTGGGGGC CTCCGAGGAC 
AGTGGGGGCC TCTATAGCTT CCTGAGCCGT CCTAACGTAC TGTCGTTCCT GAATCTCGCA 
GGCACCGACA CTGCCCTGGA CACTCTCTTC GCAGCGGTAT CCCGAGGCTG CTGCACCAGC 
CTTACCCACC TCGACGCTTC GAGGAACGTC TTCTCCCGCA CGAAGTCCCG CGCCGCGCCG 
GCCGCGCTGC AGCTCTTCCT CAGCCGCGCG CGGACGCTGC GGCACCTGGG CCTGGCGGGC 
TGCAAGCTGC CGCCCGACGC GCTCAGAGCC CTTTTGGATG GCCTCGCGCT CAACACGCAC 
CTCCGCGACC TGCACCTGGA CCTCAGCGCT TGCGAGCTGC GCTCGGCCGG CGCCCAGGTG 
ATACAAGACT TAGTGTGCGA CGCAGGCGCT GTGAGCTCCC TGGATCTGGC GGATAACGGC 
TTCGGCTCAG ACATGGTGAC TCTGGTGCTG GCCATCGGGA GAAGCCGGTC CCTGAGACAT 
GTGGCGCTTG GAAGGAACTT CAACGTCCGG TGCAAGGAGA CCCTGGACGA CGTCCTGCAC 
CGGATTGTCC AGCTCATGCA GGACGACGAT TGTCCTCTTC AGTCTCTGTC GGTGGCTGAG 
TCTCGGCTGA AGCTGGGTGC CAGCGTCCTA CTCCGGGCCC TAGCCACCAA TCCTAACCTG 
ACCGCGCTGG ATATCAGCGG CAACGCCATG GGGGACGCGG GCGCCAAGTT GCTGGCCAAG 
GCGCTGCGGG TCAACTCGAG GCTCCGGTCT GTGGTCTGGG ACCGGAACCA CACATCTGCT 
TTGGGTCTGC TGGACGTGGC GCAGGCGCTG GAGCAGAACC ACAGCCTGAA GGCCATGCCT 
CTGCCACTGA ACGACGTGGC CCAGGCGCAG CGCAGCCGCC CGGAACTGAC AGCACGTGCA 
GTCCATCAGA TCCAAGCCTG TCTCTTGAGG AACAACCGCG CAGACCCTGC CTCTTCTGAC 
CACACGACCC GCCTTCAGCC ACTTGGTCTG GTCTCAGACC CCTCAGAGCA GGAAGTGAAT 
GAATTGTGTC AGTCGGTGCA GGAGCATGTG GAGCTGCTGG GCTGTGGGGC TGGGCCCCAG 
GGTGAAGCCG CTGTGCGCCA GGCCGAGGAT GCCATCCAAA ATGCCAACTT CTCTCTCAGC 
ATTCTCCCCA TTCTATATGA AGCTGGAAGC TCCCCAAGCC ATCACTGGCA GCTTGGGCAG 
AAGCTGGAGG GCCTTCTGAG ACAGGTGGGC GAGGTCTGCC GCCAGGACAT CCAGGACTTC 
ACTCAGGCCA CACTGGACAC AGCAAGGAGC CTCTGCCCAC AGATGCTGCA GGGATCCAGC 
TGGAGGGAGC AGCTAGAGGG GGTCCTGGCA GGCTCGAGGG GCCTCCCGGA GCTGCTCCCA 
GAGCAGCTGC TGCAAGATGC CTTCACTAGG CTCAGGGACA TGCGGCTATC AATCACGGGG 
ACCTTGGCAG AGAGCATTGT GGCTCAGGCT TTGGCAGGCC TGAGTGCAGC CCGGGATCAG 
CTGGTGGAGA GTCTGGCTCA GCAGGCAACA GTGACAATGC CCCCTGCCCT ACCAGCACCG 
GATGGAGGTG AGCCCAGCCT CCTTGAGCCT GGGGAATTGG AAGGTCTTTT CTTCCCCGAG 
GAGAAGGAAG AGGAGAAGGA GAAGGATGAC AGTCCTCCAC AGAAATGGCC TGAGCTCAGC 
CACGGTCTTC ACCTGGTCCC CTTCATTCAC AGTGCTGCTG AGGAAGCGGA GCCGGAGCCC 
GAGCTGGCGG CTCCGGGAGA AGATGCAGAG CCGCAGGCGG GGCCGTCCGC GCGCGGCTCT 
CCGAGCCCTG CCGCCCCTGG GCCCCCGGCC GGCCCGCTGC CCCGCATGGA CCTGCCACTG 
GCGGGGCAGC CCCTGCGCCA TCCGACCCGG GCCCGGCCGC GGCCGCGCCG CCAGCACCAC 
CACCGCCCGC CGCCGGGGGG CCCCCAGGTA CCCCCAGCCT TGCCGCAGGA AGGGAATGGG 
CTCAGTGCCC GCGTGGACGA GGGCGTGGAG GAATTCTTCT CCAAAAGGCT GATCCAGCAG 
GATCGCCTCT GGGCCCCCGA GGAGGACCCG GCCACTGAGG GGGGCGCCAC TCCTGTCCCC 
CGTACACTGC GAAAGAAGCT GGGCACCCTC TTTGCCTTCA AGAAGCCTCG TTCAACGCGG 
GGTCCACGGA CTGATCTAGA GACCAGCCCT GGGGCAGCTC CCCGAACCCG AAAAACTACA 
TTTGGCGACC TACTGCGGCC GCCAACCCGT CCCAGCCGTG GTGAGGAGCT TGGTGGGGCT 
GAGGGGGACA CCAGCAGCCC TGACCCTGCC GGCAGGAGCC GACCTCGCTA CACAAGAGAT 
AGCAAGGCCT ACTCGATGAT ACTGCTGCCT GCCGAGGAGG AGGCAACGCT GGGTGCCAGA 
CCCGACAAGC GGCGGCCCCT GGAGCGGGGA GAAACAGAAC TGGCTCCATC CTTTGAACAG 
CGGGTACAAG TAATGCTGCA GAGGATAGGC GTCAGCCGAG GCAGCGGGGG TGCCGAAGGC 
AAGAGGAAGC AAAGCAAAGA TGGCGAGATC AAGAAAGCTG GCTCAGATGG TGACATTATG 
GACAGTTCCA CGGAGGCCCC TCCCATCTCG ATCAAGTCCC GCACCCACTC TGTGTCTGCT 
GACCCTTCCT GCAGACCTGG CCCAGGGAGC CAGGGGCCTG AGTCTGCCAC CTGGAAGACA 
CTGGGGCAGC AGTTGAATGC GGAGCTCAGG AGCCGTGGTT GGGGCCAACA GGATGGTCCA 
GGCCCTCCCT CCCCTGGTCA AAGCCCAAGT CCCTGCAGAA CCAGCCCCTC CCCAGACAGC 
CTGGGCCTCC CAGAGGACCC TTGCTTGGGC CCCAGAAATG AAGATGGCCA GCTGAGGCCG 
AGGCCTCTCT CGGCAGGGCG GCGAGCAGTG TCTGTGCATG AGGACCAGCT CCAGGCCCCT 
GCTGCCTTTC TGTGTCCCTT AGAACGGCCC CTGAGGCTGC AGCGCTCCCC CGTCCTCAAA 
CGCAGGCCAA AACTCGAGGC ACCTCCATCC CCAAGCCTAG GATCTGGCCT TGGAACCGAG 
CCTCTGCCCC CACAGCCCAC AGAGCCCTCC AGCCCTGAGC GGAGCCCACC CTCCCCAGCC 
ACAGACCAAA GAGGCGGCGG CCCCAATCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CARMIL2 ( XM_017022953.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878442.1 Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant X2, mRNA.

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CloneID	OHu85865
Clone ID Related Accession (Same CDS sequence)	XM_017022953.1
Accession Version	XM_017022953.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	capping protein, Arp2/3 and myosin-I linker protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA CCCCCGACGG CATCTCCTGT GAGCTCCGAG GCGAGATCAC CAGGTTCCTG 
TGGCCCAAAG AGGTGGAGCT GCTGCTGAAA ACCTGGCTAC CCGGGGAGGG TGCTGTGCAA 
AACCATGTCC TGGCACTGCT ACGATGGAGA GCCTACCTGC TGCACACCAC CTGCCTCCCG 
CTGAGGGTGG ACTGCACGTT CAGCTACCTG GAGGTCCAGG CCATGGCGCT GCAGGAGACA 
CCCCCTCAGG TCACCTTTGA GCTGGAGTCC CTGCGTGAGC TGGTCCTGGA GTTTCCTGGT 
GTGGCCGCCC TGGAACAGCT GGCCCAGCAC GTGGCTGCAG CCATCAAGAA GGTCTTCCCT 
CGCTCGACCC TTGGGAAGCT ATTCCGGAGG CCCACACCAG CCTCCATGCT GGCTCGGCTG 
GAGAGAAGCA GCCCCTCGGA GTCCACTGAC CCCTGCAGCC CCTGTGGTGG CTTCTTGGAG 
ACATACGAGG CTCTGTGTGA CTACAATGGC TTCCCTTTCC GAGAGGAGAT TCAGTGGGAC 
GTGGACACCA TTTACCATCG CCAGGGCTGC CGCCATTTCA GCCTGGGAGA CTTCAGCCAC 
CTCGGCAGTC GGGACCTGGC CTTGAGTGTG GCTGCCCTGT CCTACAACCT GTGGTTCCGG 
TGCCTCTCCT GTGTGGACAT GAAGCTGGTG AGGGGGTTCG GGAGCCTTGA GGTCTCAGAA 
CAGATTCTGC ACATGATGAG TCAGTCATCA CACCTGGAGG AGCTGGTGCT GGAGACCTGC 
AGCCTGAGGG GAGACTTTGT CCGACGACTG GCCCAGGCGC TGGCGGGACA CTCAAGCTCT 
GGGCTGCGGG AGCTCAGCCT CGCGGGGAAC CTGCTGGATG ACCGAGGCAT GACTGCACTC 
AGCAGACACC TCGAGCGTTG TCCAGGAGCC CTGAGGAGAC TCAGCCTGGC CCAGACAGGG 
TTGACACCGC GAGGAATGAG GGCTCTGGGC CGGGCACTGG CCACCAATGC CGCCTTCGAC 
TCCACCCTGA CCCACCTGGA CCTTTCTGGG AATCCTGGGG CGCTGGGGGC CTCCGAGGAC 
AGTGGGGGCC TCTATAGCTT CCTGAGCCGT CCTAACGTAC TGTCGTTCCT GAATCTCGCA 
GGCACCGACA CTGCCCTGGA CACTCTCTTC GCAGCGGTAT CCCGAGGCTG CTGCACCAGC 
CTTACCCACC TCGACGCTTC GAGGAACGTC TTCTCCCGCA CGAAGTCCCG CGCCGCGCCG 
GCCGCGCTGC AGCTCTTCCT CAGCCGCGCG CGGACGCTGC GGCACCTGGG CCTGGCGGGC 
TGCAAGCTGC CGCCCGACGC GCTCAGAGCC CTTTTGGATG GCCTCGCGCT CAACACGCAC 
CTCCGCGACC TGCACCTGGA CCTCAGCGCT TGCGAGCTGC GCTCGGCCGG CGCCCAGGTG 
ATACAAGACT TAGTGTGCGA CGCAGGCGCT GTGAGCTCCC TGGATCTGGC GGATAACGGC 
TTCGGCTCAG ACATGGTGAC TCTGGTGCTG GCCATCGGGA GAAGCCGGTC CCTGAGACAT 
GTGGCGCTTG GAAGGAACTT CAACGTCCGG TGCAAGGAGA CCCTGGACGA CGTCCTGCAC 
CGGATTGTCC AGCTCATGCA GGACGACGAT TGTCCTCTTC AGTCTCTGTC GGTGGCTGAG 
TCTCGGCTGA AGCTGGGTGC CAGCGTCCTA CTCCGGGCCC TAGCCACCAA TCCTAACCTG 
ACCGCGCTGG ATATCAGCGG CAACGCCATG GGGGACGCGG GCGCCAAGTT GCTGGCCAAG 
GCGCTGCGGG TCAACTCGAG GCTCCGGTCT GTGGTCTGGG ACCGGAACCA CACATCTGCT 
TTGGGTCTGC TGGACGTGGC GCAGGCGCTG GAGCAGAACC ACAGCCTGAA GGCCATGCCT 
CTGCCACTGA ACGACGTGGC CCAGGCGCAG CGCAGCCGCC CGGAACTGAC AGCACGTGCA 
GTCCATCAGA TCCAAGCCTG TCTCTTGAGG AACAACCGCG CAGACCCTGC CTCTTCTGAC 
CACACGACCC GCCTTCAGCC ACTTGGTCTG GTCTCAGACC CCTCAGAGCA GGAAGTGAAT 
GAATTGTGTC AGTCGGTGCA GGAGCATGTG GAGCTGCTGG GCTGTGGGGC TGGGCCCCAG 
GGTGAAGCCG CTGTGCGCCA GGCCGAGGAT GCCATCCAAA ATGCCAACTT CTCTCTCAGC 
ATTCTCCCCA TTCTATATGA AGCTGGAAGC TCCCCAAGCC ATCACTGGCA GCTTGGGCAG 
AAGCTGGAGG GCCTTCTGAG ACAGGTGGGC GAGGTCTGCC GCCAGGACAT CCAGGACTTC 
ACTCAGGCCA CACTGGACAC AGCAAGGAGC CTCTGCCCAC AGATGCTGCA GGGATCCAGC 
TGGAGGGAGC AGCTAGAGGG GGTCCTGGCA GGCTCGAGGG GCCTCCCGGA GCTGCTCCCA 
GAGCAGCTGC TGCAAGATGC CTTCACTAGG CTCAGGGACA TGCGGCTATC AATCACGGGG 
ACCTTGGCAG AGAGCATTGT GGCTCAGGCT TTGGCAGGCC TGAGTGCAGC CCGGGATCAG 
CTGGTGGAGA GTCTGGCTCA GCAGGCAACA GTGACAATGC CCCCTGCCCT ACCAGCACCG 
GATGGAGGTG AGCCCAGCCT CCTTGAGCCT GGGGAATTGG AAGGTCTTTT CTTCCCCGAG 
GAGAAGGAAG AGGAGAAGGA GAAGGATGAC AGTCCTCCAC AGAAATGGCC TGAGCTCAGC 
CACGGTCTTC ACCTGGTCCC CTTCATTCAC AGTGCTGCTG AGGAAGCGGA GCCGGAGCCC 
GAGCTGGCGG CTCCGGGAGA AGATGCAGAG CCGCAGGCGG GGCCGTCCGC GCGCGGCTCT 
CCGAGCCCTG CCGCCCCTGG GCCCCCGGCC GGCCCGCTGC CCCGCATGGA CCTGCCACTG 
GCGGGGCAGC CCCTGCGCCA TCCGACCCGG GCCCGGCCGC GGCCGCGCCG CCAGCACCAC 
CACCGCCCGC CGCCGGGGGG CCCCCAGGTA CCCCCAGCCT TGCCGCAGGA AGGGAATGGG 
CTCAGTGCCC GCGTGGACGA GGGCGTGGAG GAATTCTTCT CCAAAAGGCT GATCCAGCAG 
GATCGCCTCT GGGCCCCCGA GGAGGACCCG GCCACTGAGG GGGGCGCCAC TCCTGTCCCC 
CGTACACTGC GAAAGAAGCT GGGCACCCTC TTTGCCTTCA AGAAGCCTCG TTCAACGCGG 
GGTCCACGGA CTGATCTAGA GACCAGCCCT GGGGCAGCTC CCCGAACCCG AAAAACTACA 
TTTGGCGACC TACTGCGGCC GCCAACCCGT CCCAGCCGTG GTGAGGAGCT TGGTGGGGCT 
GAGGGGGACA CCAGCAGCCC TGACCCTGCC GGCAGGAGCC GACCTCGCTA CACAAGAGAT 
AGCAAGGCCT ACTCGATGAT ACTGCTGCCT GCCGAGGAGG AGGCAACGCT GGGTGCCAGA 
CCCGACAAGC GGCGGCCCCT GGAGCGGGGA GAAACAGAAC TGGCTCCATC CTTTGAACAG 
CGGGTACAAG TAATGCTGCA GAGGATAGGC GTCAGCCGAG GCAGCGGGGG TGCCGAAGGC 
AAGAGGAAGC AAAGCAAAGA TGGCGAGATC AAGAAAGCTG GCTCAGATGG TGACATTATG 
GACAGTTCCA CGGAGGCCCC TCCCATCTCG ATCAAGTCCC GCACCCACTC TGTGTCTGCT 
GACCCTTCCT GCAGACCTGG CCCAGGGAGC CAGGGGCCTG AGTCTGCCAC CTGGAAGACA 
CTGGGGCAGC AGTTGAATGC GGAGCTCAGG AGCCGTGGTT GGGGCCAACA GGATGGTCCA 
GGCCCTCCCT CCCCTGGTCA AAGCCCAAGT CCCTGCAGAA CCAGCCCCTC CCCAGACAGC 
CTGGGCCTCC CAGAGGACCC TTGCTTGGGC CCCAGAAATG AAGGGCGGCG AGCAGTGTCT 
GTGCATGAGG ACCAGCTCCA GGCCCCTGCT GCCTTTCTGT GTCCCTTAGA ACGGCCCCTG 
AGGCTGCAGC GCTCCCCCGT CCTCAAACGC AGGCCAAAAC TCGAGGCACC TCCATCCCCA 
AGCCTAGGAT CTGGCCTTGG AACCGAGCCT CTGCCCCCAC AGCCCACAGA GCCCTCCAGC 
CCTGAGCGGA GCCCACCCTC CCCAGCCACA GACCAAAGAG GCGGCGGCCC CAATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878442.1
CDS	244..4443
Translation	MAQTPDGISCELRGEITRFLWPKEVELLLKTWLPGEGAVQNHVLALLRWRAYLLHTTCLPLRVDCTFSYLEVQAMALQETPPQVTFELESLRELVLEFPGVAALEQLAQHVAAAIKKVFPRSTLGKLFRRPTPASMLARLERSSPSESTDPCSPCGGFLETYEALCDYNGFPFREEIQWDVDTIYHRQGCRHFSLGDFSHLGSRDLALSVAALSYNLWFRCLSCVDMKLVRGFGSLEVSEQILHMMSQSSHLEELVLETCSLRGDFVRRLAQALAGHSSSGLRELSLAGNLLDDRGMTALSRHLERCPGALRRLSLAQTGLTPRGMRALGRALATNAAFDSTLTHLDLSGNPGALGASEDSGGLYSFLSRPNVLSFLNLAGTDTALDTLFAAVSRGCCTSLTHLDASRNVFSRTKSRAAPAALQLFLSRARTLRHLGLAGCKLPPDALRALLDGLALNTHLRDLHLDLSACELRSAGAQVIQDLVCDAGAVSSLDLADNGFGSDMVTLVLAIGRSRSLRHVALGRNFNVRCKETLDDVLHRIVQLMQDDDCPLQSLSVAESRLKLGASVLLRALATNPNLTALDISGNAMGDAGAKLLAKALRVNSRLRSVVWDRNHTSALGLLDVAQALEQNHSLKAMPLPLNDVAQAQRSRPELTARAVHQIQACLLRNNRADPASSDHTTRLQPLGLVSDPSEQEVNELCQSVQEHVELLGCGAGPQGEAAVRQAEDAIQNANFSLSILPILYEAGSSPSHHWQLGQKLEGLLRQVGEVCRQDIQDFTQATLDTARSLCPQMLQGSSWREQLEGVLAGSRGLPELLPEQLLQDAFTRLRDMRLSITGTLAESIVAQALAGLSAARDQLVESLAQQATVTMPPALPAPDGGEPSLLEPGELEGLFFPEEKEEEKEKDDSPPQKWPELSHGLHLVPFIHSAAEEAEPEPELAAPGEDAEPQAGPSARGSPSPAAPGPPAGPLPRMDLPLAGQPLRHPTRARPRPRRQHHHRPPPGGPQVPPALPQEGNGLSARVDEGVEEFFSKRLIQQDRLWAPEEDPATEGGATPVPRTLRKKLGTLFAFKKPRSTRGPRTDLETSPGAAPRTRKTTFGDLLRPPTRPSRGEELGGAEGDTSSPDPAGRSRPRYTRDSKAYSMILLPAEEEATLGARPDKRRPLERGETELAPSFEQRVQVMLQRIGVSRGSGGAEGKRKQSKDGEIKKAGSDGDIMDSSTEAPPISIKSRTHSVSADPSCRPGPGSQGPESATWKTLGQQLNAELRSRGWGQQDGPGPPSPGQSPSPCRTSPSPDSLGLPEDPCLGPRNEGRRAVSVHEDQLQAPAAFLCPLERPLRLQRSPVLKRRPKLEAPPSPSLGSGLGTEPLPPQPTEPSSPERSPPSPATDQRGGGPNP

Target ORF information:

RefSeq Version	XM_017022953.1
Organism	Homo sapiens(human)
Definition	Homo sapiens capping protein regulator and myosin 1 linker 2 (CARMIL2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022953.1

ORF Insert Sequence:

ATGGCCCAGA CCCCCGACGG CATCTCCTGT GAGCTCCGAG GCGAGATCAC CAGGTTCCTG 
TGGCCCAAAG AGGTGGAGCT GCTGCTGAAA ACCTGGCTAC CCGGGGAGGG TGCTGTGCAA 
AACCATGTCC TGGCACTGCT ACGATGGAGA GCCTACCTGC TGCACACCAC CTGCCTCCCG 
CTGAGGGTGG ACTGCACGTT CAGCTACCTG GAGGTCCAGG CCATGGCGCT GCAGGAGACA 
CCCCCTCAGG TCACCTTTGA GCTGGAGTCC CTGCGTGAGC TGGTCCTGGA GTTTCCTGGT 
GTGGCCGCCC TGGAACAGCT GGCCCAGCAC GTGGCTGCAG CCATCAAGAA GGTCTTCCCT 
CGCTCGACCC TTGGGAAGCT ATTCCGGAGG CCCACACCAG CCTCCATGCT GGCTCGGCTG 
GAGAGAAGCA GCCCCTCGGA GTCCACTGAC CCCTGCAGCC CCTGTGGTGG CTTCTTGGAG 
ACATACGAGG CTCTGTGTGA CTACAATGGC TTCCCTTTCC GAGAGGAGAT TCAGTGGGAC 
GTGGACACCA TTTACCATCG CCAGGGCTGC CGCCATTTCA GCCTGGGAGA CTTCAGCCAC 
CTCGGCAGTC GGGACCTGGC CTTGAGTGTG GCTGCCCTGT CCTACAACCT GTGGTTCCGG 
TGCCTCTCCT GTGTGGACAT GAAGCTGGTG AGGGGGTTCG GGAGCCTTGA GGTCTCAGAA 
CAGATTCTGC ACATGATGAG TCAGTCATCA CACCTGGAGG AGCTGGTGCT GGAGACCTGC 
AGCCTGAGGG GAGACTTTGT CCGACGACTG GCCCAGGCGC TGGCGGGACA CTCAAGCTCT 
GGGCTGCGGG AGCTCAGCCT CGCGGGGAAC CTGCTGGATG ACCGAGGCAT GACTGCACTC 
AGCAGACACC TCGAGCGTTG TCCAGGAGCC CTGAGGAGAC TCAGCCTGGC CCAGACAGGG 
TTGACACCGC GAGGAATGAG GGCTCTGGGC CGGGCACTGG CCACCAATGC CGCCTTCGAC 
TCCACCCTGA CCCACCTGGA CCTTTCTGGG AATCCTGGGG CGCTGGGGGC CTCCGAGGAC 
AGTGGGGGCC TCTATAGCTT CCTGAGCCGT CCTAACGTAC TGTCGTTCCT GAATCTCGCA 
GGCACCGACA CTGCCCTGGA CACTCTCTTC GCAGCGGTAT CCCGAGGCTG CTGCACCAGC 
CTTACCCACC TCGACGCTTC GAGGAACGTC TTCTCCCGCA CGAAGTCCCG CGCCGCGCCG 
GCCGCGCTGC AGCTCTTCCT CAGCCGCGCG CGGACGCTGC GGCACCTGGG CCTGGCGGGC 
TGCAAGCTGC CGCCCGACGC GCTCAGAGCC CTTTTGGATG GCCTCGCGCT CAACACGCAC 
CTCCGCGACC TGCACCTGGA CCTCAGCGCT TGCGAGCTGC GCTCGGCCGG CGCCCAGGTG 
ATACAAGACT TAGTGTGCGA CGCAGGCGCT GTGAGCTCCC TGGATCTGGC GGATAACGGC 
TTCGGCTCAG ACATGGTGAC TCTGGTGCTG GCCATCGGGA GAAGCCGGTC CCTGAGACAT 
GTGGCGCTTG GAAGGAACTT CAACGTCCGG TGCAAGGAGA CCCTGGACGA CGTCCTGCAC 
CGGATTGTCC AGCTCATGCA GGACGACGAT TGTCCTCTTC AGTCTCTGTC GGTGGCTGAG 
TCTCGGCTGA AGCTGGGTGC CAGCGTCCTA CTCCGGGCCC TAGCCACCAA TCCTAACCTG 
ACCGCGCTGG ATATCAGCGG CAACGCCATG GGGGACGCGG GCGCCAAGTT GCTGGCCAAG 
GCGCTGCGGG TCAACTCGAG GCTCCGGTCT GTGGTCTGGG ACCGGAACCA CACATCTGCT 
TTGGGTCTGC TGGACGTGGC GCAGGCGCTG GAGCAGAACC ACAGCCTGAA GGCCATGCCT 
CTGCCACTGA ACGACGTGGC CCAGGCGCAG CGCAGCCGCC CGGAACTGAC AGCACGTGCA 
GTCCATCAGA TCCAAGCCTG TCTCTTGAGG AACAACCGCG CAGACCCTGC CTCTTCTGAC 
CACACGACCC GCCTTCAGCC ACTTGGTCTG GTCTCAGACC CCTCAGAGCA GGAAGTGAAT 
GAATTGTGTC AGTCGGTGCA GGAGCATGTG GAGCTGCTGG GCTGTGGGGC TGGGCCCCAG 
GGTGAAGCCG CTGTGCGCCA GGCCGAGGAT GCCATCCAAA ATGCCAACTT CTCTCTCAGC 
ATTCTCCCCA TTCTATATGA AGCTGGAAGC TCCCCAAGCC ATCACTGGCA GCTTGGGCAG 
AAGCTGGAGG GCCTTCTGAG ACAGGTGGGC GAGGTCTGCC GCCAGGACAT CCAGGACTTC 
ACTCAGGCCA CACTGGACAC AGCAAGGAGC CTCTGCCCAC AGATGCTGCA GGGATCCAGC 
TGGAGGGAGC AGCTAGAGGG GGTCCTGGCA GGCTCGAGGG GCCTCCCGGA GCTGCTCCCA 
GAGCAGCTGC TGCAAGATGC CTTCACTAGG CTCAGGGACA TGCGGCTATC AATCACGGGG 
ACCTTGGCAG AGAGCATTGT GGCTCAGGCT TTGGCAGGCC TGAGTGCAGC CCGGGATCAG 
CTGGTGGAGA GTCTGGCTCA GCAGGCAACA GTGACAATGC CCCCTGCCCT ACCAGCACCG 
GATGGAGGTG AGCCCAGCCT CCTTGAGCCT GGGGAATTGG AAGGTCTTTT CTTCCCCGAG 
GAGAAGGAAG AGGAGAAGGA GAAGGATGAC AGTCCTCCAC AGAAATGGCC TGAGCTCAGC 
CACGGTCTTC ACCTGGTCCC CTTCATTCAC AGTGCTGCTG AGGAAGCGGA GCCGGAGCCC 
GAGCTGGCGG CTCCGGGAGA AGATGCAGAG CCGCAGGCGG GGCCGTCCGC GCGCGGCTCT 
CCGAGCCCTG CCGCCCCTGG GCCCCCGGCC GGCCCGCTGC CCCGCATGGA CCTGCCACTG 
GCGGGGCAGC CCCTGCGCCA TCCGACCCGG GCCCGGCCGC GGCCGCGCCG CCAGCACCAC 
CACCGCCCGC CGCCGGGGGG CCCCCAGGTA CCCCCAGCCT TGCCGCAGGA AGGGAATGGG 
CTCAGTGCCC GCGTGGACGA GGGCGTGGAG GAATTCTTCT CCAAAAGGCT GATCCAGCAG 
GATCGCCTCT GGGCCCCCGA GGAGGACCCG GCCACTGAGG GGGGCGCCAC TCCTGTCCCC 
CGTACACTGC GAAAGAAGCT GGGCACCCTC TTTGCCTTCA AGAAGCCTCG TTCAACGCGG 
GGTCCACGGA CTGATCTAGA GACCAGCCCT GGGGCAGCTC CCCGAACCCG AAAAACTACA 
TTTGGCGACC TACTGCGGCC GCCAACCCGT CCCAGCCGTG GTGAGGAGCT TGGTGGGGCT 
GAGGGGGACA CCAGCAGCCC TGACCCTGCC GGCAGGAGCC GACCTCGCTA CACAAGAGAT 
AGCAAGGCCT ACTCGATGAT ACTGCTGCCT GCCGAGGAGG AGGCAACGCT GGGTGCCAGA 
CCCGACAAGC GGCGGCCCCT GGAGCGGGGA GAAACAGAAC TGGCTCCATC CTTTGAACAG 
CGGGTACAAG TAATGCTGCA GAGGATAGGC GTCAGCCGAG GCAGCGGGGG TGCCGAAGGC 
AAGAGGAAGC AAAGCAAAGA TGGCGAGATC AAGAAAGCTG GCTCAGATGG TGACATTATG 
GACAGTTCCA CGGAGGCCCC TCCCATCTCG ATCAAGTCCC GCACCCACTC TGTGTCTGCT 
GACCCTTCCT GCAGACCTGG CCCAGGGAGC CAGGGGCCTG AGTCTGCCAC CTGGAAGACA 
CTGGGGCAGC AGTTGAATGC GGAGCTCAGG AGCCGTGGTT GGGGCCAACA GGATGGTCCA 
GGCCCTCCCT CCCCTGGTCA AAGCCCAAGT CCCTGCAGAA CCAGCCCCTC CCCAGACAGC 
CTGGGCCTCC CAGAGGACCC TTGCTTGGGC CCCAGAAATG AAGGGCGGCG AGCAGTGTCT 
GTGCATGAGG ACCAGCTCCA GGCCCCTGCT GCCTTTCTGT GTCCCTTAGA ACGGCCCCTG 
AGGCTGCAGC GCTCCCCCGT CCTCAAACGC AGGCCAAAAC TCGAGGCACC TCCATCCCCA 
AGCCTAGGAT CTGGCCTTGG AACCGAGCCT CTGCCCCCAC AGCCCACAGA GCCCTCCAGC 
CCTGAGCGGA GCCCACCCTC CCCAGCCACA GACCAAAGAG GCGGCGGCCC CAATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genomic analysis of 220 CTCLs identifies a novel recurrent gain-of-function alteration in RLTPR (p.Q575E).
Blood130(12)1430-1440(2017 09)
Park J,Yang J,Wenzel AT,Ramachandran A,Lee WJ,Daniels JC,Kim J,Martinez-Escala E,Amankulor N,Pro B,Guitart J,Mendillo ML,Savas JN,Boggon TJ,Choi J

"Exome sequencing identifies a novel homozygous variant in NDRG4 in a family with infantile myofibromatosis (Linhares et?al., 2014)" turns out to be EBV+ leiomyomatosis caused by CARMIL2 mutations.
European journal of medical genetics(2017 Feb)
Linhares ND,Freire MC,Cardenas RG,Pena HB,Bahia M,Pena SD

A human immunodeficiency syndrome caused by mutations in CARMIL2.
Nature communications814209(2017 Jan)
Schober T,Magg T,Laschinger M,Rohlfs M,Linhares ND,Puchalka J,Weisser T,Fehlner K,Mautner J,Walz C,Hussein K,Jaeger G,Kammer B,Schmid I,Bahia M,Pena SD,Behrends U,Belohradsky BH,Klein C,Hauck F

A potential founder variant in
Molecular genetics & genomic medicine4(6)604-616(2016 Nov)
Sorte HS,Osnes LT,Fevang B,Aukrust P,Erichsen HC,Backe PH,Abrahamsen TG,Kittang OB,?verland T,Jhangiani SN,Muzny DM,Vigeland MD,Samarakoon P,Gambin T,Akdemir ZH,Gibbs RA,R?dningen OK,Lyle R,Lupski JR,Stray-Pedersen A

Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations.
The Journal of experimental medicine213(11)2413-2435(2016 Oct)
Wang Y,Ma CS,Ling Y,Bousfiha A,Camcioglu Y,Jacquot S,Payne K,Crestani E,Roncagalli R,Belkadi A,Kerner G,Lorenzo L,Deswarte C,Chrabieh M,Patin E,Vincent QB,M?ller-Fleckenstein I,Fleckenstein B,Ailal F,Quintana-Murci L,Fraitag S,Alyanakian MA,Leruez-Ville M,Picard C,Puel A,Bustamante J,Boisson-Dupuis S,Malissen M,Malissen B,Abel L,Hovnanian A,Notarangelo LD,Jouanguy E,Tangye SG,B?ziat V,Casanova JL

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CARMIL2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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