COL4A5 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_033380.2	NP_203699.1	collagen alpha-5(IV) chain isoform 2 precursor
NM_000495.4	NP_000486.1	collagen alpha-5(IV) chain isoform 1 precursor
XM_017029259.2	XP_016884748.1	collagen alpha-5(IV) chain isoform X2
XM_017029263.2	XP_016884752.1	collagen alpha-5(IV) chain isoform X6
NM_033380.3	NP_203699.1	collagen alpha-5(IV) chain isoform 2 precursor
NM_000495.5	NP_000486.1	collagen alpha-5(IV) chain isoform 1 precursor
XM_017029259.1	XP_016884748.1	collagen alpha-5(IV) chain isoform X2
XM_011530849.2	XP_011529151.2	collagen alpha-5(IV) chain isoform X1
XM_017029260.1	XP_016884749.1	collagen alpha-5(IV) chain isoform X3
XM_017029262.2	XP_016884751.1	collagen alpha-5(IV) chain isoform X5
XM_017029263.1	XP_016884752.1	collagen alpha-5(IV) chain isoform X6
XM_017029262.1	XP_016884751.1	collagen alpha-5(IV) chain isoform X5
XM_017029261.1	XP_016884750.1	collagen alpha-5(IV) chain isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16745	NM_033380.3 Latest version!	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu16784	NM_000495.5 Latest version!	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu99143	XM_011530849.2 Latest version!	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu99144	XM_017029259.2 Latest version!	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu99145	XM_017029260.1 Latest version!	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu99146	XM_017029261.1 Latest version!	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu99147	XM_017029262.2 Latest version!	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu99148	XM_017029263.2 Latest version!	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
View Old Accession Versions >>
OHu16745	NM_033380.2	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu16784	NM_000495.4	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu99144	XM_017029259.1	Homo sapiens collagen type IV alpha 5 (COL4A5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu99148	XM_017029263.1	Homo sapiens collagen type IV alpha 5 (COL4A5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu99147	XM_017029262.1	Homo sapiens collagen type IV alpha 5 (COL4A5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

COL4A5 ( NM_033380.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_203699.1 Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16745
Clone ID Related Accession (Same CDS sequence)	NM_033380.2 , NM_033380.3
Accession Version	NM_033380.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	collagen alpha-5(IV) chain isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA461056.1, BC151846.1, BC035387.1, M31115.1 and BU688037.1. On Dec 30, 2011 this sequence version replaced NM_033380.1. Transcript Variant: This variant (2) encodes the longer isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC151846.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCGGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCACGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCCGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
ATACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CTAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAATGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACACTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGGTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTATGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGACGGACA GCCTGGGGCT CCTGGGCTTC CAGGGCCTCC TGGCCCTGCT 
GGCCCTCACA TTCCTCCTAG TGATGAGATA TGTGAACCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AAGGGGAAAA AGGACAAGCT 
GGTGCAACTG GTCCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCCCCTG GTTTCGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCGGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGATGT AGGTCTTCCA 
GGTGACCCTG GACTTCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCCGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAGCA CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCGG GTCCTCCAGG ACGCACTGGC TTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCCGGAGCA CCTGGTCCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTAC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGT GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTTC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGCG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCCGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCCGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCATCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGGATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCACGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CTGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CTACCAGGTC CAGAAGGTCC TCCAGGTCTC 
CCTGGAAATG GAGGTATTAA AGGAGAGAAG GGAAATCCAG GCCAACCTGG GCTACCTGGC 
TTGCCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC AGGGTAATCC TGGCCGGCCG 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGATCAGCT GGCCCTGAGG GGGAACCGGG ACTTATTGGT 
CCTCCAGGTC CTCCTGGATT ACCTGGTCCT TCAGGACAGA GTATCATAAT TAAAGGAGAT 
GCTGGTCCTC CAGGAATCCC TGGCCAGCCT GGGCTAAAGG GTCTACCAGG ACCCCAAGGA 
CCTCAAGGCT TACCAGGTCC AACTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGTC TGCCAGGACA GCCAGGTACC 
CGTGGTTTGG ATGGTCCCCC TGGTCCAGAT GGATTGCAAG GTCCCCCAGG TCCCCCTGGA 
ACCTCCTCTG TTGCACATGG ATTTCTTATT ACACGCCACA GCCAGACAAC GGATGCACCA 
CAATGCCCAC AGGGAACACT TCAGGTCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CCCACGGTCA AGACTTGGGG ACGGCTGGCA GCTGCCTTCG TCGCTTTAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAC 
TATTCTTACT GGCTCTCTAC CCCAGAGCCC ATGCCAATGA GCATGCAACC CCTAAAGGGC 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCAGC TGTGGTGATC 
GCAGTTCACA GTCAGACGAT CCAGATTCCC CATTGTCCTC AGGGATGGGA TTCTCTGTGG 
ATTGGTTATT CCTTCATGAT GCATACAAGT GCAGGGGCAG AAGGCTCAGG TCAAGCCCTA 
GCCTCCCCTG GTTCCTGCTT GGAAGAGTTT CGTTCAGCTC CCTTCATCGA ATGTCATGGG 
AGGGGTACCT GTAACTACTA TGCCAACTCC TACAGCTTTT GGCTGGCAAC TGTAGATGTG 
TCAGACATGT TCAGTAAACC TCAGTCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGA 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_203699.1
CDS	283..5358
Translation	MKLRGVSLAAGLFLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVMGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPAGPHIPPSDEICEPGPPGPPGSPGDKGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGPKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGDVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGALGPKGDRGFPGPPGPPGRTGLDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGPIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPTGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPSGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGEPGPVGGGGHPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGPTGFQGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSAGPEGEPGLIGPPGPPGLPGPSGQSIIIKGDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSVAHGFLITRHSQTTDAPQCPQGTLQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	NM_033380.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033380.2

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCGGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCACGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCCGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
ATACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CTAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAATGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACACTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGGTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTATGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGACGGACA GCCTGGGGCT CCTGGGCTTC CAGGGCCTCC TGGCCCTGCT 
GGCCCTCACA TTCCTCCTAG TGATGAGATA TGTGAACCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AAGGGGAAAA AGGACAAGCT 
GGTGCAACTG GTCCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCCCCTG GTTTCGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCGGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGATGT AGGTCTTCCA 
GGTGACCCTG GACTTCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCCGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAGCA CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCGG GTCCTCCAGG ACGCACTGGC TTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCCGGAGCA CCTGGTCCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTAC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGT GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTTC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGCG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCCGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCCGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCATCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGGATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCACGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CTGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CTACCAGGTC CAGAAGGTCC TCCAGGTCTC 
CCTGGAAATG GAGGTATTAA AGGAGAGAAG GGAAATCCAG GCCAACCTGG GCTACCTGGC 
TTGCCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC AGGGTAATCC TGGCCGGCCG 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGATCAGCT GGCCCTGAGG GGGAACCGGG ACTTATTGGT 
CCTCCAGGTC CTCCTGGATT ACCTGGTCCT TCAGGACAGA GTATCATAAT TAAAGGAGAT 
GCTGGTCCTC CAGGAATCCC TGGCCAGCCT GGGCTAAAGG GTCTACCAGG ACCCCAAGGA 
CCTCAAGGCT TACCAGGTCC AACTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGTC TGCCAGGACA GCCAGGTACC 
CGTGGTTTGG ATGGTCCCCC TGGTCCAGAT GGATTGCAAG GTCCCCCAGG TCCCCCTGGA 
ACCTCCTCTG TTGCACATGG ATTTCTTATT ACACGCCACA GCCAGACAAC GGATGCACCA 
CAATGCCCAC AGGGAACACT TCAGGTCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CCCACGGTCA AGACTTGGGG ACGGCTGGCA GCTGCCTTCG TCGCTTTAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAC 
TATTCTTACT GGCTCTCTAC CCCAGAGCCC ATGCCAATGA GCATGCAACC CCTAAAGGGC 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCAGC TGTGGTGATC 
GCAGTTCACA GTCAGACGAT CCAGATTCCC CATTGTCCTC AGGGATGGGA TTCTCTGTGG 
ATTGGTTATT CCTTCATGAT GCATACAAGT GCAGGGGCAG AAGGCTCAGG TCAAGCCCTA 
GCCTCCCCTG GTTCCTGCTT GGAAGAGTTT CGTTCAGCTC CCTTCATCGA ATGTCATGGG 
AGGGGTACCT GTAACTACTA TGCCAACTCC TACAGCTTTT GGCTGGCAAC TGTAGATGTG 
TCAGACATGT TCAGTAAACC TCAGTCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGA 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( NM_033380.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_203699.1 Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 2, mRNA.

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CloneID	OHu16745
Clone ID Related Accession (Same CDS sequence)	NM_033380.2 , NM_033380.3
Accession Version	NM_033380.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	collagen alpha-5(IV) chain isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL034369.1, DA461056.1, BC151846.1, BC035387.1, M31115.1 and BU688037.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_033380.2. Transcript Variant: This variant (2) encodes the longer isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC151846.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCGGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCACGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCCGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
ATACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CTAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAATGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACACTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGGTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTATGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGACGGACA GCCTGGGGCT CCTGGGCTTC CAGGGCCTCC TGGCCCTGCT 
GGCCCTCACA TTCCTCCTAG TGATGAGATA TGTGAACCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AAGGGGAAAA AGGACAAGCT 
GGTGCAACTG GTCCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCCCCTG GTTTCGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCGGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGATGT AGGTCTTCCA 
GGTGACCCTG GACTTCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCCGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAGCA CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCGG GTCCTCCAGG ACGCACTGGC TTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCCGGAGCA CCTGGTCCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTAC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGT GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTTC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGCG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCCGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCCGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCATCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGGATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCACGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CTGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CTACCAGGTC CAGAAGGTCC TCCAGGTCTC 
CCTGGAAATG GAGGTATTAA AGGAGAGAAG GGAAATCCAG GCCAACCTGG GCTACCTGGC 
TTGCCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC AGGGTAATCC TGGCCGGCCG 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGATCAGCT GGCCCTGAGG GGGAACCGGG ACTTATTGGT 
CCTCCAGGTC CTCCTGGATT ACCTGGTCCT TCAGGACAGA GTATCATAAT TAAAGGAGAT 
GCTGGTCCTC CAGGAATCCC TGGCCAGCCT GGGCTAAAGG GTCTACCAGG ACCCCAAGGA 
CCTCAAGGCT TACCAGGTCC AACTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGTC TGCCAGGACA GCCAGGTACC 
CGTGGTTTGG ATGGTCCCCC TGGTCCAGAT GGATTGCAAG GTCCCCCAGG TCCCCCTGGA 
ACCTCCTCTG TTGCACATGG ATTTCTTATT ACACGCCACA GCCAGACAAC GGATGCACCA 
CAATGCCCAC AGGGAACACT TCAGGTCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CCCACGGTCA AGACTTGGGG ACGGCTGGCA GCTGCCTTCG TCGCTTTAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAC 
TATTCTTACT GGCTCTCTAC CCCAGAGCCC ATGCCAATGA GCATGCAACC CCTAAAGGGC 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCAGC TGTGGTGATC 
GCAGTTCACA GTCAGACGAT CCAGATTCCC CATTGTCCTC AGGGATGGGA TTCTCTGTGG 
ATTGGTTATT CCTTCATGAT GCATACAAGT GCAGGGGCAG AAGGCTCAGG TCAAGCCCTA 
GCCTCCCCTG GTTCCTGCTT GGAAGAGTTT CGTTCAGCTC CCTTCATCGA ATGTCATGGG 
AGGGGTACCT GTAACTACTA TGCCAACTCC TACAGCTTTT GGCTGGCAAC TGTAGATGTG 
TCAGACATGT TCAGTAAACC TCAGTCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGA 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_203699.1
CDS	289..5364
Translation	MKLRGVSLAAGLFLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVMGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPAGPHIPPSDEICEPGPPGPPGSPGDKGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGPKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGDVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGALGPKGDRGFPGPPGPPGRTGLDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGPIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPTGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPSGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGEPGPVGGGGHPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGPTGFQGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSAGPEGEPGLIGPPGPPGLPGPSGQSIIIKGDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSVAHGFLITRHSQTTDAPQCPQGTLQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	NM_033380.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033380.3

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCGGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCACGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCCGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
ATACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CTAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAATGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACACTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGGTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTATGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGACGGACA GCCTGGGGCT CCTGGGCTTC CAGGGCCTCC TGGCCCTGCT 
GGCCCTCACA TTCCTCCTAG TGATGAGATA TGTGAACCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AAGGGGAAAA AGGACAAGCT 
GGTGCAACTG GTCCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCCCCTG GTTTCGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCGGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGATGT AGGTCTTCCA 
GGTGACCCTG GACTTCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCCGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAGCA CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCGG GTCCTCCAGG ACGCACTGGC TTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCCGGAGCA CCTGGTCCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTAC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGT GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTTC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGCG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCCGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCCGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCATCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGGATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCACGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CTGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CTACCAGGTC CAGAAGGTCC TCCAGGTCTC 
CCTGGAAATG GAGGTATTAA AGGAGAGAAG GGAAATCCAG GCCAACCTGG GCTACCTGGC 
TTGCCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC AGGGTAATCC TGGCCGGCCG 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGATCAGCT GGCCCTGAGG GGGAACCGGG ACTTATTGGT 
CCTCCAGGTC CTCCTGGATT ACCTGGTCCT TCAGGACAGA GTATCATAAT TAAAGGAGAT 
GCTGGTCCTC CAGGAATCCC TGGCCAGCCT GGGCTAAAGG GTCTACCAGG ACCCCAAGGA 
CCTCAAGGCT TACCAGGTCC AACTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGTC TGCCAGGACA GCCAGGTACC 
CGTGGTTTGG ATGGTCCCCC TGGTCCAGAT GGATTGCAAG GTCCCCCAGG TCCCCCTGGA 
ACCTCCTCTG TTGCACATGG ATTTCTTATT ACACGCCACA GCCAGACAAC GGATGCACCA 
CAATGCCCAC AGGGAACACT TCAGGTCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CCCACGGTCA AGACTTGGGG ACGGCTGGCA GCTGCCTTCG TCGCTTTAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAC 
TATTCTTACT GGCTCTCTAC CCCAGAGCCC ATGCCAATGA GCATGCAACC CCTAAAGGGC 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCAGC TGTGGTGATC 
GCAGTTCACA GTCAGACGAT CCAGATTCCC CATTGTCCTC AGGGATGGGA TTCTCTGTGG 
ATTGGTTATT CCTTCATGAT GCATACAAGT GCAGGGGCAG AAGGCTCAGG TCAAGCCCTA 
GCCTCCCCTG GTTCCTGCTT GGAAGAGTTT CGTTCAGCTC CCTTCATCGA ATGTCATGGG 
AGGGGTACCT GTAACTACTA TGCCAACTCC TACAGCTTTT GGCTGGCAAC TGTAGATGTG 
TCAGACATGT TCAGTAAACC TCAGTCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGA 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( NM_000495.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000486.1 Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16784
Clone ID Related Accession (Same CDS sequence)	NM_000495.4 , NM_000495.5
Accession Version	NM_000495.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	collagen alpha-5(IV) chain isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA461056.1, BC151846.1, BC035387.1, M31115.1 and BU688037.1. On Dec 30, 2011 this sequence version replaced NM_000495.3. Transcript Variant: This variant (1) lacks an in-frame segment in the coding region, compared to variant 2. The resulting isoform (1) lacks two Gly-X-Y repeats, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.97910.1, SRR1803617.107038.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCGGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCACGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCCGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
ATACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CTAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAATGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACACTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGGTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTATGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGACGGACA GCCTGGGGCT CCTGGGCTTC CAGGGCCTCC TGGCCCTGCT 
GGCCCTCACA TTCCTCCTAG TGATGAGATA TGTGAACCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AAGGGGAAAA AGGACAAGCT 
GGTGCAACTG GTCCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCCCCTG GTTTCGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCGGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGATGT AGGTCTTCCA 
GGTGACCCTG GACTTCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCCGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAGCA CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCGG GTCCTCCAGG ACGCACTGGC TTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCCGGAGCA CCTGGTCCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTAC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGT GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTTC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGCG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCCGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCCGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCATCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGGATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCACGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CTGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTCTACCAGG TCCAGAAGGT CCTCCAGGTC TCCCTGGAAA TGGAGGTATT 
AAAGGAGAGA AGGGAAATCC AGGCCAACCT GGGCTACCTG GCTTGCCTGG TTTGAAAGGA 
GATCAAGGAC CACCAGGACT CCAGGGTAAT CCTGGCCGGC CGGGTCTCAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGCA TGAAAGGACC CAGTGGAGTA 
CCTGGATCAG CTGGCCCTGA GGGGGAACCG GGACTTATTG GTCCTCCAGG TCCTCCTGGA 
TTACCTGGTC CTTCAGGACA GAGTATCATA ATTAAAGGAG ATGCTGGTCC TCCAGGAATC 
CCTGGCCAGC CTGGGCTAAA GGGTCTACCA GGACCCCAAG GACCTCAAGG CTTACCAGGT 
CCAACTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA 
GGGCGCAAAG GAGACCCAGG TCTGCCAGGA CAGCCAGGTA CCCGTGGTTT GGATGGTCCC 
CCTGGTCCAG ATGGATTGCA AGGTCCCCCA GGTCCCCCTG GAACCTCCTC TGTTGCACAT 
GGATTTCTTA TTACACGCCA CAGCCAGACA ACGGATGCAC CACAATGCCC ACAGGGAACA 
CTTCAGGTCT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCCCACGGT 
CAAGACTTGG GGACGGCTGG CAGCTGCCTT CGTCGCTTTA GTACCATGCC TTTCATGTTC 
TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTCTCT 
ACCCCAGAGC CCATGCCAAT GAGCATGCAA CCCCTAAAGG GCCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAAGCTCCA GCTGTGGTGA TCGCAGTTCA CAGTCAGACG 
ATCCAGATTC CCCATTGTCC TCAGGGATGG GATTCTCTGT GGATTGGTTA TTCCTTCATG 
ATGCATACAA GTGCAGGGGC AGAAGGCTCA GGTCAAGCCC TAGCCTCCCC TGGTTCCTGC 
TTGGAAGAGT TTCGTTCAGC TCCCTTCATC GAATGTCATG GGAGGGGTAC CTGTAACTAC 
TATGCCAACT CCTACAGCTT TTGGCTGGCA ACTGTAGATG TGTCAGACAT GTTCAGTAAA 
CCTCAGTCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GAATTAGCCG ATGTCAAGTG 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000486.1
CDS	283..5340
Translation	MKLRGVSLAAGLFLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVMGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPAGPHIPPSDEICEPGPPGPPGSPGDKGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGPKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGDVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGALGPKGDRGFPGPPGPPGRTGLDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGPIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPTGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPSGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGEPGPVGGGGHPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSAGPEGEPGLIGPPGPPGLPGPSGQSIIIKGDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSVAHGFLITRHSQTTDAPQCPQGTLQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	NM_000495.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000495.4

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCGGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCACGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCCGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
ATACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CTAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAATGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACACTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGGTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTATGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGACGGACA GCCTGGGGCT CCTGGGCTTC CAGGGCCTCC TGGCCCTGCT 
GGCCCTCACA TTCCTCCTAG TGATGAGATA TGTGAACCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AAGGGGAAAA AGGACAAGCT 
GGTGCAACTG GTCCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCCCCTG GTTTCGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCGGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGATGT AGGTCTTCCA 
GGTGACCCTG GACTTCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCCGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAGCA CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCGG GTCCTCCAGG ACGCACTGGC TTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCCGGAGCA CCTGGTCCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTAC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGT GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTTC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGCG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCCGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCCGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCATCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGGATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCACGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CTGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTCTACCAGG TCCAGAAGGT CCTCCAGGTC TCCCTGGAAA TGGAGGTATT 
AAAGGAGAGA AGGGAAATCC AGGCCAACCT GGGCTACCTG GCTTGCCTGG TTTGAAAGGA 
GATCAAGGAC CACCAGGACT CCAGGGTAAT CCTGGCCGGC CGGGTCTCAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGCA TGAAAGGACC CAGTGGAGTA 
CCTGGATCAG CTGGCCCTGA GGGGGAACCG GGACTTATTG GTCCTCCAGG TCCTCCTGGA 
TTACCTGGTC CTTCAGGACA GAGTATCATA ATTAAAGGAG ATGCTGGTCC TCCAGGAATC 
CCTGGCCAGC CTGGGCTAAA GGGTCTACCA GGACCCCAAG GACCTCAAGG CTTACCAGGT 
CCAACTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA 
GGGCGCAAAG GAGACCCAGG TCTGCCAGGA CAGCCAGGTA CCCGTGGTTT GGATGGTCCC 
CCTGGTCCAG ATGGATTGCA AGGTCCCCCA GGTCCCCCTG GAACCTCCTC TGTTGCACAT 
GGATTTCTTA TTACACGCCA CAGCCAGACA ACGGATGCAC CACAATGCCC ACAGGGAACA 
CTTCAGGTCT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCCCACGGT 
CAAGACTTGG GGACGGCTGG CAGCTGCCTT CGTCGCTTTA GTACCATGCC TTTCATGTTC 
TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTCTCT 
ACCCCAGAGC CCATGCCAAT GAGCATGCAA CCCCTAAAGG GCCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAAGCTCCA GCTGTGGTGA TCGCAGTTCA CAGTCAGACG 
ATCCAGATTC CCCATTGTCC TCAGGGATGG GATTCTCTGT GGATTGGTTA TTCCTTCATG 
ATGCATACAA GTGCAGGGGC AGAAGGCTCA GGTCAAGCCC TAGCCTCCCC TGGTTCCTGC 
TTGGAAGAGT TTCGTTCAGC TCCCTTCATC GAATGTCATG GGAGGGGTAC CTGTAACTAC 
TATGCCAACT CCTACAGCTT TTGGCTGGCA ACTGTAGATG TGTCAGACAT GTTCAGTAAA 
CCTCAGTCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GAATTAGCCG ATGTCAAGTG 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( NM_000495.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000486.1 Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 1, mRNA.

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CloneID	OHu16784
Clone ID Related Accession (Same CDS sequence)	NM_000495.4 , NM_000495.5
Accession Version	NM_000495.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	collagen alpha-5(IV) chain isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL034369.1, DA461056.1, BC151846.1, BC035387.1, M31115.1 and BU688037.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_000495.4. Transcript Variant: This variant (1) lacks an in-frame segment in the coding region, compared to variant 2. The resulting isoform (1) lacks two Gly-X-Y repeats, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.198342.1, SRR1803617.107038.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCGGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCACGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCCGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
ATACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CTAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAATGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACACTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGGTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTATGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGACGGACA GCCTGGGGCT CCTGGGCTTC CAGGGCCTCC TGGCCCTGCT 
GGCCCTCACA TTCCTCCTAG TGATGAGATA TGTGAACCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AAGGGGAAAA AGGACAAGCT 
GGTGCAACTG GTCCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCCCCTG GTTTCGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCGGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGATGT AGGTCTTCCA 
GGTGACCCTG GACTTCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCCGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAGCA CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCGG GTCCTCCAGG ACGCACTGGC TTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCCGGAGCA CCTGGTCCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTAC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGT GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTTC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGCG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCCGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCCGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCATCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGGATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCACGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CTGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTCTACCAGG TCCAGAAGGT CCTCCAGGTC TCCCTGGAAA TGGAGGTATT 
AAAGGAGAGA AGGGAAATCC AGGCCAACCT GGGCTACCTG GCTTGCCTGG TTTGAAAGGA 
GATCAAGGAC CACCAGGACT CCAGGGTAAT CCTGGCCGGC CGGGTCTCAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGCA TGAAAGGACC CAGTGGAGTA 
CCTGGATCAG CTGGCCCTGA GGGGGAACCG GGACTTATTG GTCCTCCAGG TCCTCCTGGA 
TTACCTGGTC CTTCAGGACA GAGTATCATA ATTAAAGGAG ATGCTGGTCC TCCAGGAATC 
CCTGGCCAGC CTGGGCTAAA GGGTCTACCA GGACCCCAAG GACCTCAAGG CTTACCAGGT 
CCAACTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA 
GGGCGCAAAG GAGACCCAGG TCTGCCAGGA CAGCCAGGTA CCCGTGGTTT GGATGGTCCC 
CCTGGTCCAG ATGGATTGCA AGGTCCCCCA GGTCCCCCTG GAACCTCCTC TGTTGCACAT 
GGATTTCTTA TTACACGCCA CAGCCAGACA ACGGATGCAC CACAATGCCC ACAGGGAACA 
CTTCAGGTCT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCCCACGGT 
CAAGACTTGG GGACGGCTGG CAGCTGCCTT CGTCGCTTTA GTACCATGCC TTTCATGTTC 
TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTCTCT 
ACCCCAGAGC CCATGCCAAT GAGCATGCAA CCCCTAAAGG GCCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAAGCTCCA GCTGTGGTGA TCGCAGTTCA CAGTCAGACG 
ATCCAGATTC CCCATTGTCC TCAGGGATGG GATTCTCTGT GGATTGGTTA TTCCTTCATG 
ATGCATACAA GTGCAGGGGC AGAAGGCTCA GGTCAAGCCC TAGCCTCCCC TGGTTCCTGC 
TTGGAAGAGT TTCGTTCAGC TCCCTTCATC GAATGTCATG GGAGGGGTAC CTGTAACTAC 
TATGCCAACT CCTACAGCTT TTGGCTGGCA ACTGTAGATG TGTCAGACAT GTTCAGTAAA 
CCTCAGTCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GAATTAGCCG ATGTCAAGTG 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000486.1
CDS	289..5346
Translation	MKLRGVSLAAGLFLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVMGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPAGPHIPPSDEICEPGPPGPPGSPGDKGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGPKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGDVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGALGPKGDRGFPGPPGPPGRTGLDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGPIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPTGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPSGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGEPGPVGGGGHPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSAGPEGEPGLIGPPGPPGLPGPSGQSIIIKGDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSVAHGFLITRHSQTTDAPQCPQGTLQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	NM_000495.5
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000495.5

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCT CCGGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCACGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCCGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
ATACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CTAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAATGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACACTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGGTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGT 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTATGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGACGGACA GCCTGGGGCT CCTGGGCTTC CAGGGCCTCC TGGCCCTGCT 
GGCCCTCACA TTCCTCCTAG TGATGAGATA TGTGAACCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAGTGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGTTTC CCTGGACAGA AAGGGGAAAA AGGACAAGCT 
GGTGCAACTG GTCCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCCCCTG GTTTCGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCGGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGATGT AGGTCTTCCA 
GGTGACCCTG GACTTCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCCGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAGCA CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCGG GTCCTCCAGG ACGCACTGGC TTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCCGGAGCA CCTGGTCCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTAC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGT GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTTC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGCG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCCGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCCGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCATCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGGATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCACGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CTGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTCTACCAGG TCCAGAAGGT CCTCCAGGTC TCCCTGGAAA TGGAGGTATT 
AAAGGAGAGA AGGGAAATCC AGGCCAACCT GGGCTACCTG GCTTGCCTGG TTTGAAAGGA 
GATCAAGGAC CACCAGGACT CCAGGGTAAT CCTGGCCGGC CGGGTCTCAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGCA TGAAAGGACC CAGTGGAGTA 
CCTGGATCAG CTGGCCCTGA GGGGGAACCG GGACTTATTG GTCCTCCAGG TCCTCCTGGA 
TTACCTGGTC CTTCAGGACA GAGTATCATA ATTAAAGGAG ATGCTGGTCC TCCAGGAATC 
CCTGGCCAGC CTGGGCTAAA GGGTCTACCA GGACCCCAAG GACCTCAAGG CTTACCAGGT 
CCAACTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA 
GGGCGCAAAG GAGACCCAGG TCTGCCAGGA CAGCCAGGTA CCCGTGGTTT GGATGGTCCC 
CCTGGTCCAG ATGGATTGCA AGGTCCCCCA GGTCCCCCTG GAACCTCCTC TGTTGCACAT 
GGATTTCTTA TTACACGCCA CAGCCAGACA ACGGATGCAC CACAATGCCC ACAGGGAACA 
CTTCAGGTCT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCCCACGGT 
CAAGACTTGG GGACGGCTGG CAGCTGCCTT CGTCGCTTTA GTACCATGCC TTTCATGTTC 
TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTCTCT 
ACCCCAGAGC CCATGCCAAT GAGCATGCAA CCCCTAAAGG GCCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAAGCTCCA GCTGTGGTGA TCGCAGTTCA CAGTCAGACG 
ATCCAGATTC CCCATTGTCC TCAGGGATGG GATTCTCTGT GGATTGGTTA TTCCTTCATG 
ATGCATACAA GTGCAGGGGC AGAAGGCTCA GGTCAAGCCC TAGCCTCCCC TGGTTCCTGC 
TTGGAAGAGT TTCGTTCAGC TCCCTTCATC GAATGTCATG GGAGGGGTAC CTGTAACTAC 
TATGCCAACT CCTACAGCTT TTGGCTGGCA ACTGTAGATG TGTCAGACAT GTTCAGTAAA 
CCTCAGTCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GAATTAGCCG ATGTCAAGTG 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_011530849.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529151.2 Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X1, mRNA.

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CloneID	OHu99143
Clone ID Related Accession (Same CDS sequence)	XM_011530849.2
Accession Version	XM_011530849.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-5(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA 
TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG 
GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA 
CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC 
CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG 
GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT 
CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT 
CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG 
GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT 
CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT 
GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT 
GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC 
ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA 
GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT 
CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT 
CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA 
ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG 
CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT 
CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT 
GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA 
CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG 
GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA 
GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA 
ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTCCT ACAGGTTTTC AAGGTCTACC AGGTCCAGAA 
GGTCCTCCAG GTCTCCCTGG AAATGGAGGT ATTAAAGGAG AGAAGGGAAA TCCAGGCCAA 
CCTGGGCTAC CTGGCTTGCC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCAGGGT 
AATCCTGGCC GGCCGGGTCT CAATGGAATG AAAGGAGATC CTGGTCTCCC TGGTGTTCCA 
GGATTCCCAG GCATGAAAGG ACCCAGTGGA GTACCTGGAT CAGCTGGCCC TGAGGGGGAA 
CCGGGACTTA TTGGTCCTCC AGGTCCTCCT GGATTACCTG GTCCTTCAGG ACAGAGTATC 
ATAATTAAAG GAGATGCTGG TCCTCCAGGA ATCCCTGGCC AGCCTGGGCT AAAGGGTCTA 
CCAGGACCCC AAGGACCTCA AGGCTTACCA GGTCCAACTG GCCCTCCAGG AGATCCTGGA 
CGCAATGGAC TCCCTGGCTT TGATGGTGCA GGAGGGCGCA AAGGAGACCC AGGTCTGCCA 
GGACAGCCAG GTACCCGTGG TTTGGATGGT CCCCCTGGTC CAGATGGATT GCAAGGTCCC 
CCAGGTCCCC CTGGAACCTC CTCTGTTGCA CATGGATTTC TTATTACACG CCACAGCCAG 
ACAACGGATG CACCACAATG CCCACAGGGA ACACTTCAGG TCTATGAAGG CTTTTCTCTC 
CTGTATGTAC AAGGAAATAA AAGAGCCCAC GGTCAAGACT TGGGGACGGC TGGCAGCTGC 
CTTCGTCGCT TTAGTACCAT GCCTTTCATG TTCTGCAACA TCAATAATGT TTGCAACTTT 
GCTTCAAGAA ATGACTATTC TTACTGGCTC TCTACCCCAG AGCCCATGCC AATGAGCATG 
CAACCCCTAA AGGGCCAGAG CATCCAGCCA TTCATTAGTC GATGTGCAGT ATGTGAAGCT 
CCAGCTGTGG TGATCGCAGT TCACAGTCAG ACGATCCAGA TTCCCCATTG TCCTCAGGGA 
TGGGATTCTC TGTGGATTGG TTATTCCTTC ATGATGCATA CAAGTGCAGG GGCAGAAGGC 
TCAGGTCAAG CCCTAGCCTC CCCTGGTTCC TGCTTGGAAG AGTTTCGTTC AGCTCCCTTC 
ATCGAATGTC ATGGGAGGGG TACCTGTAAC TACTATGCCA ACTCCTACAG CTTTTGGCTG 
GCAACTGTAG ATGTGTCAGA CATGTTCAGT AAACCTCAGT CAGAAACGCT GAAAGCAGGA 
GACTTGAGGA CACGAATTAG CCGATGTCAA GTGTGCATGA AGAGGACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529151.2
CDS	16..5106
Translation	MEVDSGKTENRDWEGFCYSTSAYWKNLYDGLLACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVMGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPAGPHIPPSDEICEPGPPGPPGSPGDKGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGPKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGDVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGALGPKGDRGFPGPPGPPGRTGLDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGPIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPTGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPSGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGEPGPVGGGGHPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGPTGFQGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSAGPEGEPGLIGPPGPPGLPGPSGQSIIIKGDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSVAHGFLITRHSQTTDAPQCPQGTLQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_011530849.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530849.2

ORF Insert Sequence:

ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA 
TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG 
GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA 
CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC 
CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG 
GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT 
CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT 
CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG 
GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT 
CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT 
GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT 
GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC 
ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA 
GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT 
CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT 
CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA 
ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG 
CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT 
CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT 
GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA 
CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG 
GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA 
GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA 
ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTCCT ACAGGTTTTC AAGGTCTACC AGGTCCAGAA 
GGTCCTCCAG GTCTCCCTGG AAATGGAGGT ATTAAAGGAG AGAAGGGAAA TCCAGGCCAA 
CCTGGGCTAC CTGGCTTGCC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCAGGGT 
AATCCTGGCC GGCCGGGTCT CAATGGAATG AAAGGAGATC CTGGTCTCCC TGGTGTTCCA 
GGATTCCCAG GCATGAAAGG ACCCAGTGGA GTACCTGGAT CAGCTGGCCC TGAGGGGGAA 
CCGGGACTTA TTGGTCCTCC AGGTCCTCCT GGATTACCTG GTCCTTCAGG ACAGAGTATC 
ATAATTAAAG GAGATGCTGG TCCTCCAGGA ATCCCTGGCC AGCCTGGGCT AAAGGGTCTA 
CCAGGACCCC AAGGACCTCA AGGCTTACCA GGTCCAACTG GCCCTCCAGG AGATCCTGGA 
CGCAATGGAC TCCCTGGCTT TGATGGTGCA GGAGGGCGCA AAGGAGACCC AGGTCTGCCA 
GGACAGCCAG GTACCCGTGG TTTGGATGGT CCCCCTGGTC CAGATGGATT GCAAGGTCCC 
CCAGGTCCCC CTGGAACCTC CTCTGTTGCA CATGGATTTC TTATTACACG CCACAGCCAG 
ACAACGGATG CACCACAATG CCCACAGGGA ACACTTCAGG TCTATGAAGG CTTTTCTCTC 
CTGTATGTAC AAGGAAATAA AAGAGCCCAC GGTCAAGACT TGGGGACGGC TGGCAGCTGC 
CTTCGTCGCT TTAGTACCAT GCCTTTCATG TTCTGCAACA TCAATAATGT TTGCAACTTT 
GCTTCAAGAA ATGACTATTC TTACTGGCTC TCTACCCCAG AGCCCATGCC AATGAGCATG 
CAACCCCTAA AGGGCCAGAG CATCCAGCCA TTCATTAGTC GATGTGCAGT ATGTGAAGCT 
CCAGCTGTGG TGATCGCAGT TCACAGTCAG ACGATCCAGA TTCCCCATTG TCCTCAGGGA 
TGGGATTCTC TGTGGATTGG TTATTCCTTC ATGATGCATA CAAGTGCAGG GGCAGAAGGC 
TCAGGTCAAG CCCTAGCCTC CCCTGGTTCC TGCTTGGAAG AGTTTCGTTC AGCTCCCTTC 
ATCGAATGTC ATGGGAGGGG TACCTGTAAC TACTATGCCA ACTCCTACAG CTTTTGGCTG 
GCAACTGTAG ATGTGTCAGA CATGTTCAGT AAACCTCAGT CAGAAACGCT GAAAGCAGGA 
GACTTGAGGA CACGAATTAG CCGATGTCAA GTGTGCATGA AGAGGACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_017029259.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884748.1 Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X2, mRNA.

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CloneID	OHu99144
Clone ID Related Accession (Same CDS sequence)	XM_017029259.2 , XM_017029259.1
Accession Version	XM_017029259.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-5(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029259.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA 
TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG 
GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA 
CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC 
CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG 
GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT 
CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT 
CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG 
GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT 
CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT 
GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT 
GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC 
ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA 
GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT 
CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT 
CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA 
ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG 
CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT 
CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT 
GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA 
CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG 
GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA 
GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA 
ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTTTT CAAGGTCTAC CAGGTCCAGA AGGTCCTCCA 
GGTCTCCCTG GAAATGGAGG TATTAAAGGA GAGAAGGGAA ATCCAGGCCA ACCTGGGCTA 
CCTGGCTTGC CTGGTTTGAA AGGAGATCAA GGACCACCAG GACTCCAGGG TAATCCTGGC 
CGGCCGGGTC TCAATGGAAT GAAAGGAGAT CCTGGTCTCC CTGGTGTTCC AGGATTCCCA 
GGCATGAAAG GACCCAGTGG AGTACCTGGA TCAGCTGGCC CTGAGGGGGA ACCGGGACTT 
ATTGGTCCTC CAGGTCCTCC TGGATTACCT GGTCCTTCAG GACAGAGTAT CATAATTAAA 
GGAGATGCTG GTCCTCCAGG AATCCCTGGC CAGCCTGGGC TAAAGGGTCT ACCAGGACCC 
CAAGGACCTC AAGGCTTACC AGGTCCAACT GGCCCTCCAG GAGATCCTGG ACGCAATGGA 
CTCCCTGGCT TTGATGGTGC AGGAGGGCGC AAAGGAGACC CAGGTCTGCC AGGACAGCCA 
GGTACCCGTG GTTTGGATGG TCCCCCTGGT CCAGATGGAT TGCAAGGTCC CCCAGGTCCC 
CCTGGAACCT CCTCTGTTGC ACATGGATTT CTTATTACAC GCCACAGCCA GACAACGGAT 
GCACCACAAT GCCCACAGGG AACACTTCAG GTCTATGAAG GCTTTTCTCT CCTGTATGTA 
CAAGGAAATA AAAGAGCCCA CGGTCAAGAC TTGGGGACGG CTGGCAGCTG CCTTCGTCGC 
TTTAGTACCA TGCCTTTCAT GTTCTGCAAC ATCAATAATG TTTGCAACTT TGCTTCAAGA 
AATGACTATT CTTACTGGCT CTCTACCCCA GAGCCCATGC CAATGAGCAT GCAACCCCTA 
AAGGGCCAGA GCATCCAGCC ATTCATTAGT CGATGTGCAG TATGTGAAGC TCCAGCTGTG 
GTGATCGCAG TTCACAGTCA GACGATCCAG ATTCCCCATT GTCCTCAGGG ATGGGATTCT 
CTGTGGATTG GTTATTCCTT CATGATGCAT ACAAGTGCAG GGGCAGAAGG CTCAGGTCAA 
GCCCTAGCCT CCCCTGGTTC CTGCTTGGAA GAGTTTCGTT CAGCTCCCTT CATCGAATGT 
CATGGGAGGG GTACCTGTAA CTACTATGCC AACTCCTACA GCTTTTGGCT GGCAACTGTA 
GATGTGTCAG ACATGTTCAG TAAACCTCAG TCAGAAACGC TGAAAGCAGG AGACTTGAGG 
ACACGAATTA GCCGATGTCA AGTGTGCATG AAGAGGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884748.1
CDS	16..5097
Translation	MEVDSGKTENRDWEGFCYSTSAYWKNLYDGLLACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVMGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPAGPHIPPSDEICEPGPPGPPGSPGDKGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGPKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGDVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGALGPKGDRGFPGPPGPPGRTGLDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGPIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPTGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPSGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGEPGPVGGGGHPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGFQGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSAGPEGEPGLIGPPGPPGLPGPSGQSIIIKGDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSVAHGFLITRHSQTTDAPQCPQGTLQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_017029259.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029259.2

ORF Insert Sequence:

ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA 
TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG 
GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA 
CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC 
CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG 
GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT 
CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT 
CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG 
GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT 
CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT 
GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT 
GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC 
ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA 
GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT 
CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT 
CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA 
ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG 
CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT 
CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT 
GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA 
CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG 
GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA 
GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA 
ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTTTT CAAGGTCTAC CAGGTCCAGA AGGTCCTCCA 
GGTCTCCCTG GAAATGGAGG TATTAAAGGA GAGAAGGGAA ATCCAGGCCA ACCTGGGCTA 
CCTGGCTTGC CTGGTTTGAA AGGAGATCAA GGACCACCAG GACTCCAGGG TAATCCTGGC 
CGGCCGGGTC TCAATGGAAT GAAAGGAGAT CCTGGTCTCC CTGGTGTTCC AGGATTCCCA 
GGCATGAAAG GACCCAGTGG AGTACCTGGA TCAGCTGGCC CTGAGGGGGA ACCGGGACTT 
ATTGGTCCTC CAGGTCCTCC TGGATTACCT GGTCCTTCAG GACAGAGTAT CATAATTAAA 
GGAGATGCTG GTCCTCCAGG AATCCCTGGC CAGCCTGGGC TAAAGGGTCT ACCAGGACCC 
CAAGGACCTC AAGGCTTACC AGGTCCAACT GGCCCTCCAG GAGATCCTGG ACGCAATGGA 
CTCCCTGGCT TTGATGGTGC AGGAGGGCGC AAAGGAGACC CAGGTCTGCC AGGACAGCCA 
GGTACCCGTG GTTTGGATGG TCCCCCTGGT CCAGATGGAT TGCAAGGTCC CCCAGGTCCC 
CCTGGAACCT CCTCTGTTGC ACATGGATTT CTTATTACAC GCCACAGCCA GACAACGGAT 
GCACCACAAT GCCCACAGGG AACACTTCAG GTCTATGAAG GCTTTTCTCT CCTGTATGTA 
CAAGGAAATA AAAGAGCCCA CGGTCAAGAC TTGGGGACGG CTGGCAGCTG CCTTCGTCGC 
TTTAGTACCA TGCCTTTCAT GTTCTGCAAC ATCAATAATG TTTGCAACTT TGCTTCAAGA 
AATGACTATT CTTACTGGCT CTCTACCCCA GAGCCCATGC CAATGAGCAT GCAACCCCTA 
AAGGGCCAGA GCATCCAGCC ATTCATTAGT CGATGTGCAG TATGTGAAGC TCCAGCTGTG 
GTGATCGCAG TTCACAGTCA GACGATCCAG ATTCCCCATT GTCCTCAGGG ATGGGATTCT 
CTGTGGATTG GTTATTCCTT CATGATGCAT ACAAGTGCAG GGGCAGAAGG CTCAGGTCAA 
GCCCTAGCCT CCCCTGGTTC CTGCTTGGAA GAGTTTCGTT CAGCTCCCTT CATCGAATGT 
CATGGGAGGG GTACCTGTAA CTACTATGCC AACTCCTACA GCTTTTGGCT GGCAACTGTA 
GATGTGTCAG ACATGTTCAG TAAACCTCAG TCAGAAACGC TGAAAGCAGG AGACTTGAGG 
ACACGAATTA GCCGATGTCA AGTGTGCATG AAGAGGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_017029259.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884748.1 Homo sapiens collagen type IV alpha 5 (COL4A5), transcript variant X2, mRNA.

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CloneID	OHu99144
Clone ID Related Accession (Same CDS sequence)	XM_017029259.2 , XM_017029259.1
Accession Version	XM_017029259.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-5(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA 
TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG 
GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA 
CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC 
CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG 
GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT 
CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT 
CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG 
GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT 
CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT 
GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT 
GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC 
ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA 
GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT 
CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT 
CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA 
ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG 
CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT 
CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT 
GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA 
CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG 
GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA 
GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA 
ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTTTT CAAGGTCTAC CAGGTCCAGA AGGTCCTCCA 
GGTCTCCCTG GAAATGGAGG TATTAAAGGA GAGAAGGGAA ATCCAGGCCA ACCTGGGCTA 
CCTGGCTTGC CTGGTTTGAA AGGAGATCAA GGACCACCAG GACTCCAGGG TAATCCTGGC 
CGGCCGGGTC TCAATGGAAT GAAAGGAGAT CCTGGTCTCC CTGGTGTTCC AGGATTCCCA 
GGCATGAAAG GACCCAGTGG AGTACCTGGA TCAGCTGGCC CTGAGGGGGA ACCGGGACTT 
ATTGGTCCTC CAGGTCCTCC TGGATTACCT GGTCCTTCAG GACAGAGTAT CATAATTAAA 
GGAGATGCTG GTCCTCCAGG AATCCCTGGC CAGCCTGGGC TAAAGGGTCT ACCAGGACCC 
CAAGGACCTC AAGGCTTACC AGGTCCAACT GGCCCTCCAG GAGATCCTGG ACGCAATGGA 
CTCCCTGGCT TTGATGGTGC AGGAGGGCGC AAAGGAGACC CAGGTCTGCC AGGACAGCCA 
GGTACCCGTG GTTTGGATGG TCCCCCTGGT CCAGATGGAT TGCAAGGTCC CCCAGGTCCC 
CCTGGAACCT CCTCTGTTGC ACATGGATTT CTTATTACAC GCCACAGCCA GACAACGGAT 
GCACCACAAT GCCCACAGGG AACACTTCAG GTCTATGAAG GCTTTTCTCT CCTGTATGTA 
CAAGGAAATA AAAGAGCCCA CGGTCAAGAC TTGGGGACGG CTGGCAGCTG CCTTCGTCGC 
TTTAGTACCA TGCCTTTCAT GTTCTGCAAC ATCAATAATG TTTGCAACTT TGCTTCAAGA 
AATGACTATT CTTACTGGCT CTCTACCCCA GAGCCCATGC CAATGAGCAT GCAACCCCTA 
AAGGGCCAGA GCATCCAGCC ATTCATTAGT CGATGTGCAG TATGTGAAGC TCCAGCTGTG 
GTGATCGCAG TTCACAGTCA GACGATCCAG ATTCCCCATT GTCCTCAGGG ATGGGATTCT 
CTGTGGATTG GTTATTCCTT CATGATGCAT ACAAGTGCAG GGGCAGAAGG CTCAGGTCAA 
GCCCTAGCCT CCCCTGGTTC CTGCTTGGAA GAGTTTCGTT CAGCTCCCTT CATCGAATGT 
CATGGGAGGG GTACCTGTAA CTACTATGCC AACTCCTACA GCTTTTGGCT GGCAACTGTA 
GATGTGTCAG ACATGTTCAG TAAACCTCAG TCAGAAACGC TGAAAGCAGG AGACTTGAGG 
ACACGAATTA GCCGATGTCA AGTGTGCATG AAGAGGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884748.1
CDS	16..5097
Translation	MEVDSGKTENRDWEGFCYSTSAYWKNLYDGLLACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVMGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPAGPHIPPSDEICEPGPPGPPGSPGDKGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGPKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGDVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGALGPKGDRGFPGPPGPPGRTGLDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGPIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPTGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPSGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGEPGPVGGGGHPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGFQGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSAGPEGEPGLIGPPGPPGLPGPSGQSIIIKGDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSVAHGFLITRHSQTTDAPQCPQGTLQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_017029259.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 5 (COL4A5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029259.1

ORF Insert Sequence:

ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA 
TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG 
GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA 
CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC 
CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG 
GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT 
CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT 
CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG 
GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT 
CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT 
GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT 
GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC 
ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA 
GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT 
CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT 
CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA 
ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG 
CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT 
CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT 
GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA 
CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG 
GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA 
GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA 
ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTTTT CAAGGTCTAC CAGGTCCAGA AGGTCCTCCA 
GGTCTCCCTG GAAATGGAGG TATTAAAGGA GAGAAGGGAA ATCCAGGCCA ACCTGGGCTA 
CCTGGCTTGC CTGGTTTGAA AGGAGATCAA GGACCACCAG GACTCCAGGG TAATCCTGGC 
CGGCCGGGTC TCAATGGAAT GAAAGGAGAT CCTGGTCTCC CTGGTGTTCC AGGATTCCCA 
GGCATGAAAG GACCCAGTGG AGTACCTGGA TCAGCTGGCC CTGAGGGGGA ACCGGGACTT 
ATTGGTCCTC CAGGTCCTCC TGGATTACCT GGTCCTTCAG GACAGAGTAT CATAATTAAA 
GGAGATGCTG GTCCTCCAGG AATCCCTGGC CAGCCTGGGC TAAAGGGTCT ACCAGGACCC 
CAAGGACCTC AAGGCTTACC AGGTCCAACT GGCCCTCCAG GAGATCCTGG ACGCAATGGA 
CTCCCTGGCT TTGATGGTGC AGGAGGGCGC AAAGGAGACC CAGGTCTGCC AGGACAGCCA 
GGTACCCGTG GTTTGGATGG TCCCCCTGGT CCAGATGGAT TGCAAGGTCC CCCAGGTCCC 
CCTGGAACCT CCTCTGTTGC ACATGGATTT CTTATTACAC GCCACAGCCA GACAACGGAT 
GCACCACAAT GCCCACAGGG AACACTTCAG GTCTATGAAG GCTTTTCTCT CCTGTATGTA 
CAAGGAAATA AAAGAGCCCA CGGTCAAGAC TTGGGGACGG CTGGCAGCTG CCTTCGTCGC 
TTTAGTACCA TGCCTTTCAT GTTCTGCAAC ATCAATAATG TTTGCAACTT TGCTTCAAGA 
AATGACTATT CTTACTGGCT CTCTACCCCA GAGCCCATGC CAATGAGCAT GCAACCCCTA 
AAGGGCCAGA GCATCCAGCC ATTCATTAGT CGATGTGCAG TATGTGAAGC TCCAGCTGTG 
GTGATCGCAG TTCACAGTCA GACGATCCAG ATTCCCCATT GTCCTCAGGG ATGGGATTCT 
CTGTGGATTG GTTATTCCTT CATGATGCAT ACAAGTGCAG GGGCAGAAGG CTCAGGTCAA 
GCCCTAGCCT CCCCTGGTTC CTGCTTGGAA GAGTTTCGTT CAGCTCCCTT CATCGAATGT 
CATGGGAGGG GTACCTGTAA CTACTATGCC AACTCCTACA GCTTTTGGCT GGCAACTGTA 
GATGTGTCAG ACATGTTCAG TAAACCTCAG TCAGAAACGC TGAAAGCAGG AGACTTGAGG 
ACACGAATTA GCCGATGTCA AGTGTGCATG AAGAGGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_017029260.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884749.1 Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X3, mRNA.

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CloneID	OHu99145
Clone ID Related Accession (Same CDS sequence)	XM_017029260.1
Accession Version	XM_017029260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-5(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA 
TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG 
GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA 
CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC 
CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG 
GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT 
CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT 
CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG 
GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT 
CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT 
GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT 
GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC 
ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA 
GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT 
CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT 
CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA 
ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG 
CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT 
CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT 
GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA 
CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG 
GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA 
GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA 
ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTCTA CCAGGTCCAG AAGGTCCTCC AGGTCTCCCT 
GGAAATGGAG GTATTAAAGG AGAGAAGGGA AATCCAGGCC AACCTGGGCT ACCTGGCTTG 
CCTGGTTTGA AAGGAGATCA AGGACCACCA GGACTCCAGG GTAATCCTGG CCGGCCGGGT 
CTCAATGGAA TGAAAGGAGA TCCTGGTCTC CCTGGTGTTC CAGGATTCCC AGGCATGAAA 
GGACCCAGTG GAGTACCTGG ATCAGCTGGC CCTGAGGGGG AACCGGGACT TATTGGTCCT 
CCAGGTCCTC CTGGATTACC TGGTCCTTCA GGACAGAGTA TCATAATTAA AGGAGATGCT 
GGTCCTCCAG GAATCCCTGG CCAGCCTGGG CTAAAGGGTC TACCAGGACC CCAAGGACCT 
CAAGGCTTAC CAGGTCCAAC TGGCCCTCCA GGAGATCCTG GACGCAATGG ACTCCCTGGC 
TTTGATGGTG CAGGAGGGCG CAAAGGAGAC CCAGGTCTGC CAGGACAGCC AGGTACCCGT 
GGTTTGGATG GTCCCCCTGG TCCAGATGGA TTGCAAGGTC CCCCAGGTCC CCCTGGAACC 
TCCTCTGTTG CACATGGATT TCTTATTACA CGCCACAGCC AGACAACGGA TGCACCACAA 
TGCCCACAGG GAACACTTCA GGTCTATGAA GGCTTTTCTC TCCTGTATGT ACAAGGAAAT 
AAAAGAGCCC ACGGTCAAGA CTTGGGGACG GCTGGCAGCT GCCTTCGTCG CTTTAGTACC 
ATGCCTTTCA TGTTCTGCAA CATCAATAAT GTTTGCAACT TTGCTTCAAG AAATGACTAT 
TCTTACTGGC TCTCTACCCC AGAGCCCATG CCAATGAGCA TGCAACCCCT AAAGGGCCAG 
AGCATCCAGC CATTCATTAG TCGATGTGCA GTATGTGAAG CTCCAGCTGT GGTGATCGCA 
GTTCACAGTC AGACGATCCA GATTCCCCAT TGTCCTCAGG GATGGGATTC TCTGTGGATT 
GGTTATTCCT TCATGATGCA TACAAGTGCA GGGGCAGAAG GCTCAGGTCA AGCCCTAGCC 
TCCCCTGGTT CCTGCTTGGA AGAGTTTCGT TCAGCTCCCT TCATCGAATG TCATGGGAGG 
GGTACCTGTA ACTACTATGC CAACTCCTAC AGCTTTTGGC TGGCAACTGT AGATGTGTCA 
GACATGTTCA GTAAACCTCA GTCAGAAACG CTGAAAGCAG GAGACTTGAG GACACGAATT 
AGCCGATGTC AAGTGTGCAT GAAGAGGACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884749.1
CDS	16..5088
Translation	MEVDSGKTENRDWEGFCYSTSAYWKNLYDGLLACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVMGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPAGPHIPPSDEICEPGPPGPPGSPGDKGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGPKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGDVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGALGPKGDRGFPGPPGPPGRTGLDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGPIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPTGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPSGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGEPGPVGGGGHPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSAGPEGEPGLIGPPGPPGLPGPSGQSIIIKGDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSVAHGFLITRHSQTTDAPQCPQGTLQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_017029260.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029260.1

ORF Insert Sequence:

ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA 
TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG 
GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA 
CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC 
CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG 
GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT 
CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT 
CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG 
GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT 
CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT 
GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT 
GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC 
ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA 
GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT 
CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT 
CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA 
ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG 
CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT 
CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT 
GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA 
CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG 
GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA 
GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA 
ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTCTA CCAGGTCCAG AAGGTCCTCC AGGTCTCCCT 
GGAAATGGAG GTATTAAAGG AGAGAAGGGA AATCCAGGCC AACCTGGGCT ACCTGGCTTG 
CCTGGTTTGA AAGGAGATCA AGGACCACCA GGACTCCAGG GTAATCCTGG CCGGCCGGGT 
CTCAATGGAA TGAAAGGAGA TCCTGGTCTC CCTGGTGTTC CAGGATTCCC AGGCATGAAA 
GGACCCAGTG GAGTACCTGG ATCAGCTGGC CCTGAGGGGG AACCGGGACT TATTGGTCCT 
CCAGGTCCTC CTGGATTACC TGGTCCTTCA GGACAGAGTA TCATAATTAA AGGAGATGCT 
GGTCCTCCAG GAATCCCTGG CCAGCCTGGG CTAAAGGGTC TACCAGGACC CCAAGGACCT 
CAAGGCTTAC CAGGTCCAAC TGGCCCTCCA GGAGATCCTG GACGCAATGG ACTCCCTGGC 
TTTGATGGTG CAGGAGGGCG CAAAGGAGAC CCAGGTCTGC CAGGACAGCC AGGTACCCGT 
GGTTTGGATG GTCCCCCTGG TCCAGATGGA TTGCAAGGTC CCCCAGGTCC CCCTGGAACC 
TCCTCTGTTG CACATGGATT TCTTATTACA CGCCACAGCC AGACAACGGA TGCACCACAA 
TGCCCACAGG GAACACTTCA GGTCTATGAA GGCTTTTCTC TCCTGTATGT ACAAGGAAAT 
AAAAGAGCCC ACGGTCAAGA CTTGGGGACG GCTGGCAGCT GCCTTCGTCG CTTTAGTACC 
ATGCCTTTCA TGTTCTGCAA CATCAATAAT GTTTGCAACT TTGCTTCAAG AAATGACTAT 
TCTTACTGGC TCTCTACCCC AGAGCCCATG CCAATGAGCA TGCAACCCCT AAAGGGCCAG 
AGCATCCAGC CATTCATTAG TCGATGTGCA GTATGTGAAG CTCCAGCTGT GGTGATCGCA 
GTTCACAGTC AGACGATCCA GATTCCCCAT TGTCCTCAGG GATGGGATTC TCTGTGGATT 
GGTTATTCCT TCATGATGCA TACAAGTGCA GGGGCAGAAG GCTCAGGTCA AGCCCTAGCC 
TCCCCTGGTT CCTGCTTGGA AGAGTTTCGT TCAGCTCCCT TCATCGAATG TCATGGGAGG 
GGTACCTGTA ACTACTATGC CAACTCCTAC AGCTTTTGGC TGGCAACTGT AGATGTGTCA 
GACATGTTCA GTAAACCTCA GTCAGAAACG CTGAAAGCAG GAGACTTGAG GACACGAATT 
AGCCGATGTC AAGTGTGCAT GAAGAGGACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_017029261.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884750.1 Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X4, mRNA.

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CloneID	OHu99146
Clone ID Related Accession (Same CDS sequence)	XM_017029261.1
Accession Version	XM_017029261.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-5(IV) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA 
TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG 
GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA 
CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC 
CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG 
GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT 
CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT 
CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG 
GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT 
CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT 
GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT 
GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC 
ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA 
GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT 
CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT 
CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA 
ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG 
CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT 
CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT 
GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA 
CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG 
GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA 
GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA 
ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTCCT ACAGGTTTTC AAGGTCTACC AGGTCCAGAA 
GGTCCTCCAG GTCTCCCTGG AAATGGAGGT ATTAAAGGAG AGAAGGGAAA TCCAGGCCAA 
CCTGGGCTAC CTGGCTTGCC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCAGTGG 
TTTGACTTGA TTGTACAAAG GGAGATCCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884750.1
CDS	16..4008
Translation	MEVDSGKTENRDWEGFCYSTSAYWKNLYDGLLACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVMGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPAGPHIPPSDEICEPGPPGPPGSPGDKGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGPKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGDVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGALGPKGDRGFPGPPGPPGRTGLDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGPIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPTGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPSGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGEPGPVGGGGHPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGPTGFQGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQWFDLIVQREIL

Target ORF information:

RefSeq Version	XM_017029261.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029261.1

ORF Insert Sequence:

ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA 
TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG 
GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA 
CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC 
CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG 
GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT 
CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT 
CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG 
GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT 
CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT 
GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT 
GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC 
ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA 
GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT 
CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT 
CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA 
ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG 
CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT 
CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT 
GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA 
CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG 
GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA 
GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA 
ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTCCT ACAGGTTTTC AAGGTCTACC AGGTCCAGAA 
GGTCCTCCAG GTCTCCCTGG AAATGGAGGT ATTAAAGGAG AGAAGGGAAA TCCAGGCCAA 
CCTGGGCTAC CTGGCTTGCC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCAGTGG 
TTTGACTTGA TTGTACAAAG GGAGATCCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_017029262.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884751.1 Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu99147
Clone ID Related Accession (Same CDS sequence)	XM_017029262.2 , XM_017029262.1
Accession Version	XM_017029262.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-5(IV) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029262.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA 
TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG 
GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA 
CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC 
CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG 
GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT 
CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT 
CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG 
GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT 
CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT 
GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT 
GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC 
ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA 
GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT 
CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT 
CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA 
ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG 
CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT 
CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT 
GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA 
CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG 
GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA 
GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA 
ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTTTT CAAACTATCC CACCTTCACA TTTTGGGTTG 
TCACAAAGTT CTCAATTCTA CTGCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884751.1
CDS	16..3885
Translation	MEVDSGKTENRDWEGFCYSTSAYWKNLYDGLLACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVMGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPAGPHIPPSDEICEPGPPGPPGSPGDKGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGPKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGDVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGALGPKGDRGFPGPPGPPGRTGLDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGPIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPTGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPSGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGEPGPVGGGGHPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGFQTIPPSHFGLSQSSQFYCC

Target ORF information:

RefSeq Version	XM_017029262.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029262.2

ORF Insert Sequence:

ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA 
TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG 
GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA 
CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC 
CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG 
GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT 
CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT 
CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG 
GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT 
CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT 
GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT 
GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC 
ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA 
GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT 
CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT 
CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA 
ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG 
CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT 
CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT 
GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA 
CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG 
GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA 
GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA 
ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTTTT CAAACTATCC CACCTTCACA TTTTGGGTTG 
TCACAAAGTT CTCAATTCTA CTGCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_017029262.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884751.1 Homo sapiens collagen type IV alpha 5 (COL4A5), transcript variant X5, mRNA.

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CloneID	OHu99147
Clone ID Related Accession (Same CDS sequence)	XM_017029262.2 , XM_017029262.1
Accession Version	XM_017029262.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-5(IV) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA 
TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG 
GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA 
CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC 
CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG 
GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT 
CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT 
CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG 
GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT 
CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT 
GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT 
GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC 
ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA 
GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT 
CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT 
CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA 
ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG 
CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT 
CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT 
GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA 
CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG 
GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA 
GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA 
ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTTTT CAAACTATCC CACCTTCACA TTTTGGGTTG 
TCACAAAGTT CTCAATTCTA CTGCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884751.1
CDS	16..3885
Translation	MEVDSGKTENRDWEGFCYSTSAYWKNLYDGLLACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVMGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPAGPHIPPSDEICEPGPPGPPGSPGDKGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGPKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGDVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGALGPKGDRGFPGPPGPPGRTGLDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGPIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPTGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPSGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGEPGPVGGGGHPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGFQTIPPSHFGLSQSSQFYCC

Target ORF information:

RefSeq Version	XM_017029262.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 5 (COL4A5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029262.1

ORF Insert Sequence:

ATGGAGGTAG ACAGTGGAAA GACAGAGAAC AGAGACTGGG AAGGATTCTG CTATTCAACT 
TCAGCTTACT GGAAGAATCT TTACGATGGG TTGCTGGCTT GCTATGGGTG TTCTCCAGGA 
TCAAAGTGTG ACTGCAGTGG CATAAAAGGG GAAAAGGGAG AGAGAGGGTT TCCAGGTTTG 
GAAGGACACC CAGGATTGCC TGGATTTCCA GGTCCAGAAG GGCCTCCGGG GCCTCGGGGA 
CAAAAGGGTG ATGATGGAAT TCCAGGGCCA CCAGGACCAA AAGGAATCAG AGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGAA TGCCAGGCCA CGATGGGGCC 
CCAGGACCTC AAGGTATTCC CGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCCGGTT TTCCTGGTTT ACAGGGTCCT CCAGGACCCC CTGGGATCCC AGGTATGAAG 
GGTGAACCAG GTAGTATAAT TATGTCATCA CTGCCAGGAC CAAAGGGTAA TCCAGGATAT 
CCAGGTCCTC CTGGAATACA AGGCCTACCT GGTCCCACTG GTATACCAGG GCCAATTGGT 
CCCCCAGGAC CACCAGGTTT GATGGGCCCT CCTGGTCCAC CAGGACTTCC AGGACCTAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGTGAGCA AGGTCTTCAG 
GGCCCACCTG GGCCACCTGG GCAGATCAGT GAACAGAAAA GACCAATTGA TGTAGAGTTT 
CAGAAAGGAG ATCAGGGACT TCCTGGTGAC CGAGGGCCTC CTGGACCTCC AGGGATACGT 
GGTCCTCCAG GTCCCCCAGG TGGTGAGAAA GGTGAGAAGG GTGAGCAAGG AGAGCCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAA AATGGCCAAC CAGGAATTCC TGGTTTGCCT 
GGTGATCCTG GTTACCCTGG TGAACCCGGA AGGGATGGTG AAAAGGGCCA AAAAGGTGAC 
ACTGGCCCAC CTGGACCTCC TGGACTTGTA ATTCCTAGAC CTGGGACTGG TATAACTATA 
GGAGAAAAAG GAAACATTGG GTTGCCTGGG TTGCCTGGAG AAAAAGGAGA GCGAGGATTT 
CCTGGAATAC AGGGTCCACC TGGCCTTCCT GGACCTCCAG GGGCTGCAGT TATGGGTCCT 
CCTGGCCCTC CTGGATTTCC TGGAGAAAGG GGTCAGAAAG GTGATGAAGG ACCACCTGGA 
ATTTCCATTC CTGGACCTCC TGGACTTGAC GGACAGCCTG GGGCTCCTGG GCTTCCAGGG 
CCTCCTGGCC CTGCTGGCCC TCACATTCCT CCTAGTGATG AGATATGTGA ACCAGGCCCT 
CCAGGCCCCC CAGGATCTCC AGGTGATAAA GGACTCCAAG GAGAACAAGG AGTGAAAGGT 
GACAAAGGTG ACACTTGCTT CAACTGCATT GGAACTGGTA TTTCAGGGCC TCCAGGTCAA 
CCTGGTTTGC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GTTTCCCTGG ACAGAAAGGG 
GAAAAAGGAC AAGCTGGTGC AACTGGTCCC AAAGGATTAC CAGGCATTCC AGGAGCTCCA 
GGTGCTCCAG GCTTTCCTGG ATCTAAAGGT GAACCTGGTG ATATCCTCAC TTTTCCAGGA 
ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTTTT CAAACTATCC CACCTTCACA TTTTGGGTTG 
TCACAAAGTT CTCAATTCTA CTGCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_017029263.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884752.1 Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu99148
Clone ID Related Accession (Same CDS sequence)	XM_017029263.2 , XM_017029263.1
Accession Version	XM_017029263.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-5(IV) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029263.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTCCT ACAGGTTTTC AAGGTCTACC AGGTCCAGAA 
GGTCCTCCAG GTCTCCCTGG AAATGGAGGT ATTAAAGGAG AGAAGGGAAA TCCAGGCCAA 
CCTGGGCTAC CTGGCTTGCC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCAGGGT 
AATCCTGGCC GGCCGGGTCT CAATGGAATG AAAGGAGATC CTGGTCTCCC TGGTGTTCCA 
GGATTCCCAG GCATGAAAGG ACCCAGTGGA GTACCTGGAT CAGCTGGCCC TGAGGGGGAA 
CCGGGACTTA TTGGTCCTCC AGGTCCTCCT GGATTACCTG GTCCTTCAGG ACAGAGTATC 
ATAATTAAAG GAGATGCTGG TCCTCCAGGA ATCCCTGGCC AGCCTGGGCT AAAGGGTCTA 
CCAGGACCCC AAGGACCTCA AGGCTTACCA GGTCCAACTG GCCCTCCAGG AGATCCTGGA 
CGCAATGGAC TCCCTGGCTT TGATGGTGCA GGAGGGCGCA AAGGAGACCC AGGTCTGCCA 
GGACAGCCAG GTACCCGTGG TTTGGATGGT CCCCCTGGTC CAGATGGATT GCAAGGTCCC 
CCAGGTCCCC CTGGAACCTC CTCTGTTGCA CATGGATTTC TTATTACACG CCACAGCCAG 
ACAACGGATG CACCACAATG CCCACAGGGA ACACTTCAGG TCTATGAAGG CTTTTCTCTC 
CTGTATGTAC AAGGAAATAA AAGAGCCCAC GGTCAAGACT TGGGGACGGC TGGCAGCTGC 
CTTCGTCGCT TTAGTACCAT GCCTTTCATG TTCTGCAACA TCAATAATGT TTGCAACTTT 
GCTTCAAGAA ATGACTATTC TTACTGGCTC TCTACCCCAG AGCCCATGCC AATGAGCATG 
CAACCCCTAA AGGGCCAGAG CATCCAGCCA TTCATTAGTC GATGTGCAGT ATGTGAAGCT 
CCAGCTGTGG TGATCGCAGT TCACAGTCAG ACGATCCAGA TTCCCCATTG TCCTCAGGGA 
TGGGATTCTC TGTGGATTGG TTATTCCTTC ATGATGCATA CAAGTGCAGG GGCAGAAGGC 
TCAGGTCAAG CCCTAGCCTC CCCTGGTTCC TGCTTGGAAG AGTTTCGTTC AGCTCCCTTC 
ATCGAATGTC ATGGGAGGGG TACCTGTAAC TACTATGCCA ACTCCTACAG CTTTTGGCTG 
GCAACTGTAG ATGTGTCAGA CATGTTCAGT AAACCTCAGT CAGAAACGCT GAAAGCAGGA 
GACTTGAGGA CACGAATTAG CCGATGTCAA GTGTGCATGA AGAGGACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884752.1
CDS	780..4190
Translation	MKGDKGELGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGDVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGALGPKGDRGFPGPPGPPGRTGLDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGPIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPTGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPSGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGEPGPVGGGGHPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGPTGFQGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSAGPEGEPGLIGPPGPPGLPGPSGQSIIIKGDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSVAHGFLITRHSQTTDAPQCPQGTLQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_017029263.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 5 chain (COL4A5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029263.2

ORF Insert Sequence:

ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTCCT ACAGGTTTTC AAGGTCTACC AGGTCCAGAA 
GGTCCTCCAG GTCTCCCTGG AAATGGAGGT ATTAAAGGAG AGAAGGGAAA TCCAGGCCAA 
CCTGGGCTAC CTGGCTTGCC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCAGGGT 
AATCCTGGCC GGCCGGGTCT CAATGGAATG AAAGGAGATC CTGGTCTCCC TGGTGTTCCA 
GGATTCCCAG GCATGAAAGG ACCCAGTGGA GTACCTGGAT CAGCTGGCCC TGAGGGGGAA 
CCGGGACTTA TTGGTCCTCC AGGTCCTCCT GGATTACCTG GTCCTTCAGG ACAGAGTATC 
ATAATTAAAG GAGATGCTGG TCCTCCAGGA ATCCCTGGCC AGCCTGGGCT AAAGGGTCTA 
CCAGGACCCC AAGGACCTCA AGGCTTACCA GGTCCAACTG GCCCTCCAGG AGATCCTGGA 
CGCAATGGAC TCCCTGGCTT TGATGGTGCA GGAGGGCGCA AAGGAGACCC AGGTCTGCCA 
GGACAGCCAG GTACCCGTGG TTTGGATGGT CCCCCTGGTC CAGATGGATT GCAAGGTCCC 
CCAGGTCCCC CTGGAACCTC CTCTGTTGCA CATGGATTTC TTATTACACG CCACAGCCAG 
ACAACGGATG CACCACAATG CCCACAGGGA ACACTTCAGG TCTATGAAGG CTTTTCTCTC 
CTGTATGTAC AAGGAAATAA AAGAGCCCAC GGTCAAGACT TGGGGACGGC TGGCAGCTGC 
CTTCGTCGCT TTAGTACCAT GCCTTTCATG TTCTGCAACA TCAATAATGT TTGCAACTTT 
GCTTCAAGAA ATGACTATTC TTACTGGCTC TCTACCCCAG AGCCCATGCC AATGAGCATG 
CAACCCCTAA AGGGCCAGAG CATCCAGCCA TTCATTAGTC GATGTGCAGT ATGTGAAGCT 
CCAGCTGTGG TGATCGCAGT TCACAGTCAG ACGATCCAGA TTCCCCATTG TCCTCAGGGA 
TGGGATTCTC TGTGGATTGG TTATTCCTTC ATGATGCATA CAAGTGCAGG GGCAGAAGGC 
TCAGGTCAAG CCCTAGCCTC CCCTGGTTCC TGCTTGGAAG AGTTTCGTTC AGCTCCCTTC 
ATCGAATGTC ATGGGAGGGG TACCTGTAAC TACTATGCCA ACTCCTACAG CTTTTGGCTG 
GCAACTGTAG ATGTGTCAGA CATGTTCAGT AAACCTCAGT CAGAAACGCT GAAAGCAGGA 
GACTTGAGGA CACGAATTAG CCGATGTCAA GTGTGCATGA AGAGGACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_017029263.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884752.1 Homo sapiens collagen type IV alpha 5 (COL4A5), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu99148
Clone ID Related Accession (Same CDS sequence)	XM_017029263.2 , XM_017029263.1
Accession Version	XM_017029263.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-5(IV) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTCCT ACAGGTTTTC AAGGTCTACC AGGTCCAGAA 
GGTCCTCCAG GTCTCCCTGG AAATGGAGGT ATTAAAGGAG AGAAGGGAAA TCCAGGCCAA 
CCTGGGCTAC CTGGCTTGCC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCAGGGT 
AATCCTGGCC GGCCGGGTCT CAATGGAATG AAAGGAGATC CTGGTCTCCC TGGTGTTCCA 
GGATTCCCAG GCATGAAAGG ACCCAGTGGA GTACCTGGAT CAGCTGGCCC TGAGGGGGAA 
CCGGGACTTA TTGGTCCTCC AGGTCCTCCT GGATTACCTG GTCCTTCAGG ACAGAGTATC 
ATAATTAAAG GAGATGCTGG TCCTCCAGGA ATCCCTGGCC AGCCTGGGCT AAAGGGTCTA 
CCAGGACCCC AAGGACCTCA AGGCTTACCA GGTCCAACTG GCCCTCCAGG AGATCCTGGA 
CGCAATGGAC TCCCTGGCTT TGATGGTGCA GGAGGGCGCA AAGGAGACCC AGGTCTGCCA 
GGACAGCCAG GTACCCGTGG TTTGGATGGT CCCCCTGGTC CAGATGGATT GCAAGGTCCC 
CCAGGTCCCC CTGGAACCTC CTCTGTTGCA CATGGATTTC TTATTACACG CCACAGCCAG 
ACAACGGATG CACCACAATG CCCACAGGGA ACACTTCAGG TCTATGAAGG CTTTTCTCTC 
CTGTATGTAC AAGGAAATAA AAGAGCCCAC GGTCAAGACT TGGGGACGGC TGGCAGCTGC 
CTTCGTCGCT TTAGTACCAT GCCTTTCATG TTCTGCAACA TCAATAATGT TTGCAACTTT 
GCTTCAAGAA ATGACTATTC TTACTGGCTC TCTACCCCAG AGCCCATGCC AATGAGCATG 
CAACCCCTAA AGGGCCAGAG CATCCAGCCA TTCATTAGTC GATGTGCAGT ATGTGAAGCT 
CCAGCTGTGG TGATCGCAGT TCACAGTCAG ACGATCCAGA TTCCCCATTG TCCTCAGGGA 
TGGGATTCTC TGTGGATTGG TTATTCCTTC ATGATGCATA CAAGTGCAGG GGCAGAAGGC 
TCAGGTCAAG CCCTAGCCTC CCCTGGTTCC TGCTTGGAAG AGTTTCGTTC AGCTCCCTTC 
ATCGAATGTC ATGGGAGGGG TACCTGTAAC TACTATGCCA ACTCCTACAG CTTTTGGCTG 
GCAACTGTAG ATGTGTCAGA CATGTTCAGT AAACCTCAGT CAGAAACGCT GAAAGCAGGA 
GACTTGAGGA CACGAATTAG CCGATGTCAA GTGTGCATGA AGAGGACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884752.1
CDS	774..4184
Translation	MKGDKGELGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGDVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGALGPKGDRGFPGPPGPPGRTGLDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGPIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPTGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPSGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGEPGPVGGGGHPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGPTGFQGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSAGPEGEPGLIGPPGPPGLPGPSGQSIIIKGDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSVAHGFLITRHSQTTDAPQCPQGTLQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_017029263.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 5 (COL4A5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029263.1

ORF Insert Sequence:

ATGAAGGGTG ACAAAGGAGA GTTGGGTTCC CCTGGAGCTC CAGGGCTTCC TGGTTTACCT 
GGCACTCCTG GACAGGATGG ATTGCCAGGG CTTCCTGGCC CGAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCCCCT GGGAACCCAG GTTTACCAGG CCTCCCAGGG 
AATATAGGGC CTATGGGTCC CCCTGGTTTC GGCCCTCCAG GCCCAGTAGG TGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCAGGAATAC CAGGTCCTAA AGGGGATCCA 
GGTCAGACTA TAACCCAGCC GGGGAAGCCT GGCTTGCCTG GTAACCCAGG CAGAGATGGT 
GATGTAGGTC TTCCAGGTGA CCCTGGACTT CCAGGGCAAC CAGGCTTGCC AGGGATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGA ATTGGGCTTC CTGGACCACC TGGTCCCAAA 
GGCTTTCCTG GAATTCCAGG ACCTCCAGGA GCACCTGGGA CACCTGGAAG AATTGGTCTA 
GAAGGCCCTC CTGGGCCACC CGGCTTTCCA GGACCAAAGG GTGAACCAGG ATTTGCATTA 
CCTGGGCCAC CTGGGCCACC AGGACTTCCA GGTTTCAAAG GAGCACTTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TCCGGGTCCT CCAGGACGCA CTGGCTTAGA TGGGCTCCCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGAC CAATGGGACC TCCTGGGCTG 
CCAGGAATAG GTGTTCAGGG ACCACCAGGA CCACCAGGGA TTCCTGGGCC AATAGGTCAA 
CCTGGTTTAC ATGGAATACC AGGAGAGAAG GGGGATCCAG GACCTCCTGG ACTTGATGTT 
CCAGGACCCC CAGGTGAAAG AGGCAGTCCA GGGATCCCCG GAGCACCTGG TCCTATAGGA 
CCTCCAGGAT CACCAGGGCT TCCAGGAAAA GCAGGTGCCT CTGGATTTCC AGGTACCAAA 
GGTGAAATGG GTATGATGGG ACCTCCAGGC CCACCAGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGTAC CTGGTCTTAA AGGTGATGAT GGCTTGCAGG GTCAGCCAGG ACTTCCTGGC 
CCTACAGGAG AAAAAGGTAG TAAAGGAGAG CCTGGCCTTC CAGGCCCTCC TGGACCAATG 
GATCCAAATC TTCTGGGCTC AAAAGGAGAG AAGGGGGAAC CTGGCTTACC AGGTATACCT 
GGAGTTTCAG GGCCAAAAGG TTATCAGGGT TTGCCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CTGGATTACC AGGACCACCA GGTCCCAAAG GTAACCCTGG TCTCCCTGGA 
CAGCCAGGTC TTATAGGACC TCCTGGACTT AAAGGAACCA TCGGTGATAT GGGTTTTCCA 
GGGCCTCAGG GTGTGGAAGG GCCTCCTGGA CCTTCTGGAG TTCCTGGACA ACCTGGCTCC 
CCAGGATTAC CTGGACAGAA AGGCGACAAA GGTGATCCTG GTATTTCAAG CATTGGTCTT 
CCAGGTCTTC CTGGTCCAAA GGGTGAGCCT GGTCTGCCTG GATACCCAGG GAACCCTGGT 
ATCAAAGGTT CTGTGGGAGA TCCTGGTTTG CCCGGATTAC CAGGAACCCC TGGAGCAAAA 
GGACAACCAG GCCTTCCTGG ATTCCCAGGA ACCCCAGGCC CTCCTGGACC AAAAGGTATT 
AGTGGCCCTC CTGGGAACCC CGGCCTTCCA GGAGAACCTG GTCCTGTAGG TGGTGGAGGT 
CATCCTGGGC AACCAGGGCC TCCAGGCGAA AAAGGCAAAC CCGGTCAAGA TGGTATTCCT 
GGACCAGCTG GACAGAAGGG TGAACCAGGT CAACCAGGCT TTGGAAACCC AGGACCCCCT 
GGACTTCCAG GACTTTCTGG CCAAAAGGGT GATGGAGGAT TACCTGGGAT TCCAGGAAAT 
CCTGGCCTTC CAGGTCCAAA GGGCGAACCA GGCTTTCACG GTTTCCCTGG TGTGCAGGGT 
CCCCCAGGCC CTCCTGGTTC TCCGGGTCCA GCTCTGGAAG GACCTAAAGG CAACCCTGGG 
CCCCAAGGTC CTCCTGGGAG ACCAGGTCCT ACAGGTTTTC AAGGTCTACC AGGTCCAGAA 
GGTCCTCCAG GTCTCCCTGG AAATGGAGGT ATTAAAGGAG AGAAGGGAAA TCCAGGCCAA 
CCTGGGCTAC CTGGCTTGCC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCAGGGT 
AATCCTGGCC GGCCGGGTCT CAATGGAATG AAAGGAGATC CTGGTCTCCC TGGTGTTCCA 
GGATTCCCAG GCATGAAAGG ACCCAGTGGA GTACCTGGAT CAGCTGGCCC TGAGGGGGAA 
CCGGGACTTA TTGGTCCTCC AGGTCCTCCT GGATTACCTG GTCCTTCAGG ACAGAGTATC 
ATAATTAAAG GAGATGCTGG TCCTCCAGGA ATCCCTGGCC AGCCTGGGCT AAAGGGTCTA 
CCAGGACCCC AAGGACCTCA AGGCTTACCA GGTCCAACTG GCCCTCCAGG AGATCCTGGA 
CGCAATGGAC TCCCTGGCTT TGATGGTGCA GGAGGGCGCA AAGGAGACCC AGGTCTGCCA 
GGACAGCCAG GTACCCGTGG TTTGGATGGT CCCCCTGGTC CAGATGGATT GCAAGGTCCC 
CCAGGTCCCC CTGGAACCTC CTCTGTTGCA CATGGATTTC TTATTACACG CCACAGCCAG 
ACAACGGATG CACCACAATG CCCACAGGGA ACACTTCAGG TCTATGAAGG CTTTTCTCTC 
CTGTATGTAC AAGGAAATAA AAGAGCCCAC GGTCAAGACT TGGGGACGGC TGGCAGCTGC 
CTTCGTCGCT TTAGTACCAT GCCTTTCATG TTCTGCAACA TCAATAATGT TTGCAACTTT 
GCTTCAAGAA ATGACTATTC TTACTGGCTC TCTACCCCAG AGCCCATGCC AATGAGCATG 
CAACCCCTAA AGGGCCAGAG CATCCAGCCA TTCATTAGTC GATGTGCAGT ATGTGAAGCT 
CCAGCTGTGG TGATCGCAGT TCACAGTCAG ACGATCCAGA TTCCCCATTG TCCTCAGGGA 
TGGGATTCTC TGTGGATTGG TTATTCCTTC ATGATGCATA CAAGTGCAGG GGCAGAAGGC 
TCAGGTCAAG CCCTAGCCTC CCCTGGTTCC TGCTTGGAAG AGTTTCGTTC AGCTCCCTTC 
ATCGAATGTC ATGGGAGGGG TACCTGTAAC TACTATGCCA ACTCCTACAG CTTTTGGCTG 
GCAACTGTAG ATGTGTCAGA CATGTTCAGT AAACCTCAGT CAGAAACGCT GAAAGCAGGA 
GACTTGAGGA CACGAATTAG CCGATGTCAA GTGTGCATGA AGAGGACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	COL4A5
Entrez Gene ID	1287
Full Name	collagen type IV alpha 5 chain
Synonyms	ASLN,ATS,CA54
General protein information	Preferred Names collagen type IV alpha 5 chain Names collagen alpha-5(IV) chain collagen IV, alpha-5 polypeptide collagen of basement membrane, alpha-5 chain collagen, type IV, alpha 5 dA149D17.3 dA24A23.1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq22.3
Summary	This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010].
Disorder MIM:	303630
Disorder Html:	Alport syndrome, 301050 (3)

Pan troglodytes (chimpanzee)	COL4A5	XP_003317667.2
Homo sapiens (human)	COL4A5	NP_000486.1
Mus musculus (house mouse)	Col4a5	NP_031762.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

COL4A5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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