Col4a5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col4a5
Entrez Gene ID	12830
Full Name	collagen, type IV, alpha 5
General protein information	Preferred Names collagen, type IV, alpha 5 Names collagen alpha-5(IV) chain collagen type IV alpha5 chain procollagen type IV alpha 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X F2\|X 62.16 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_007736.4	NP_031762.2	collagen alpha-5(IV) chain precursor
XM_017318372.2	XP_017173861.1	collagen alpha-5(IV) chain isoform X3
NM_001163155.1	NP_001156627.1	collagen alpha-5(IV) chain precursor
XM_006528697.3	XP_006528760.1	collagen alpha-5(IV) chain isoform X2
XM_017318372.1	XP_017173861.1	collagen alpha-5(IV) chain isoform X3
XM_006528696.3	XP_006528759.1	collagen alpha-5(IV) chain isoform X1
XM_006528697.4	XP_006528760.1	collagen alpha-5(IV) chain isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL4A5	XP_003317667.2
Homo sapiens (human)	COL4A5	NP_000486.1
Mus musculus (house mouse)	Col4a5	NP_031762.2

Gene Ontology
Bibliography

Related articles in PubMed

Pathogenicity of a Human Laminin
Funk SD, Bayer RH, Malone AF, McKee KK, Yurchenco PD, Miner JH
Journal of the American Society of Nephrology : JASN(2017 Dec)

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

A step towards clinical application of acellular matrix: A clue from macrophage polarization.
Petrosyan A, Da Sacco S, Tripuraneni N, Kreuser U, Lavarreda-Pearce M, Tamburrini R, De Filippo RE, Orlando G, Cravedi P, Perin L
Matrix biology : journal of the International Society for Matrix Biology57-58334-346(2017 Jan)

A potential target gene for the host-directed therapy of mycobacterial infection in murine macrophages.
Bao Z, Chen R, Zhang P, Lu S, Chen X, Yao Y, Jin X, Sun Y, Zhou J
International journal of molecular medicine38(3)823-33(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings indicate a role for collagen alpha-5(IV) chain (Col4a5) in the regulation of macrophage responses to mycobacterial infection and identify Col4a5 as a potential target for the host-directed anti-mycobacterial therapy.
Title: A potential target gene for the host-directed therapy of mycobacterial infection in murine macrophages.

alpha5(IV), but not alpha1(IV), promotes lung cancer cell proliferation and tumor angiogenesis through non-integrin collagen receptor DDR1-mediated ERK activation.
Title: Minor Type IV Collagen ?5 Chain Promotes Cancer Progression through Discoidin Domain Receptor-1.

Report novel mouse model of Alport syndrome with accumulation abnormal collagen alpha3alpha4alpha5(IV) trimers in the glomerular basement membrane.
Title: A mouse Col4a4 mutation causing Alport glomerulosclerosis with abnormal collagen ?3?4?5(IV) trimers.

the proximal bifunctional promoter regulates the expression of the alpha5(IV) and alpha6(IV) genes in a cell-specific manner and demonstrates promoter plasticity in growth factor regulation of type IV collagen genes in different tissues of the body.
Title: Bifunctional promoter of type IV collagen COL4A5 and COL4A6 genes regulates the expression of alpha5 and alpha6 chains in a distinct cell-specific fashion.

Alpha3(IV), alpha4(IV), and alpha5(IV) chains form a complex, which is a heterotrimer, and a defect in complex formation might be one of the molecular mechanisms underlying the pathogenesis of Alport syndrome.
Title: Characterization of assembly of recombinant type IV collagen alpha3, alpha4, and alpha5 chains in transfected cell strains.

The following Col4a5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col4a5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu56584	XM_006528697.4 Latest version!	Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu83066	XM_017318372.2 Latest version!	Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu00702	NM_007736.4 Latest version!	Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OMu00702	NM_001163155.1 Latest version!	Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OMu56583	XM_006528696.3 Latest version!	Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OMu83066	XM_017318372.1	Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu56584	XM_006528697.3	Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col4a5 ( XM_006528697.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528760.1 Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant X2, mRNA.

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CloneID	OMu56584
Clone ID Related Accession (Same CDS sequence)	XM_006528697.3 , XM_006528697.4
Accession Version	XM_006528697.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-5(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528697.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTGC GTGGAGTGTG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCCG CAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGGTTTCCAG GTTTAGAAGG TCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGCCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGGATGCCA GGCCATGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC GGGCTTTCCT 
GGTTTACAAG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGGGA ACCAGGCAGT 
ATAATCATGT CACCACTGCC AGGACCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGCC CACCTGGTCC CAAGGGTATA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GACCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGC GAACAAGGTC TTCAGGGCCC ACCTGGTCCA 
CCTGGGCAGA TCAGCGAACA GAAAAGACCA ATTGACATAG AATTTCAGAA AGGAGATCAG 
GGAGTTCCTG GTGATAGAGG GCCTCCTGGA CCTCCAGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCAGGAGAGG CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG CCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGAGAAC CAGGAAGAGA TGGTGAAAAG GGTCAAAAGG GTGATATTGG CCCACCTGGA 
CCTCCCGGAC TTGTAATTCC TGCACCTGGA ACTGGTGTGA CTATGGGAGA AAAAGGAAAT 
ATCGGGTTAC CTGGTTTGCC TGGAGAAAAG GGAGAGCGAG GATTTCCTGG AATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGAACA GCAGTTGTGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGCCTTC CAGGACCTCC TGGCCCCCCT 
GGCCCCCAGT TGCCATCCAG AGATGAAATC TGTAAAGCAG GCCCTCCTGG GCCTCCAGGA 
CCTCCAGGTG ATAAAGGACT CCAAGGAGAG CGAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCTGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGGGACAA AGGACAAGCT 
GGGATAACTG GTCCGAAAGG ATTGCCAGGC ATACCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGGGATGTC CTCACTCTTC CAGGAATGAA AGGTGACAAA 
GGAGAGTTGG GTTTCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTCCC TGGCCCCAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CTCCTGGGAG CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCAGTTG GTTTTGGGCC TCCAGGCCCC ATAGGCGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCGGG ATTACCAGGT CCTAAAGGAG ATCCAGGCCA GACCATAACC 
CAGCCAGGAA AGCCTGGTTT GCCAGGTAGT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCTGGA CCACCTGGTC CTAAAGGTTT TCCAGGAATT 
CCGGGACCTC CAGGAGCTCC TGGGGCCCCT GGAAGAATGG GTCCAGATGG GCCTCCTGGG 
CCACCAGGCT TCCCAGGACC AAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTTCAG GTCCTCCAGG ACGCACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGCCCAGTA GGGCCTCCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGG 
ATTCCAGGAG AGAAGGGGGA CCCAGGACCT CCTGGGTTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTGTCCCTG GAAAAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC TGGACCTTTG GGAATTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGATGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGC AGGAGAAAAG 
GGCGGTAAGG GAGAGCCTGG CCTTCCAGGT CCTCCTGGAC CAATGCATCC AGATTTCCTG 
GGATCGAAAG GAGAAAAGGG TGAGCCCGGC TTACCAGGTA TTCCTGGACT TTCAGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCAGGA 
TCACCAGGAC TCCCAGGTCC CAAGGGTAAT CCTGGTCTCC CTGGTCCACC AGGACTTACA 
GGACCTCCTG GACCAAAAGG AAACATTGGT GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCCC CTGGACCTCC TGGATTTCCT GGACAACCTG GCTCTCCTGG GTTACCTGGC 
CAGAAAGGAA GTAAAGGAGA GCCTGGGGTT TCAGGCATTG GACTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGGTCGGTG 
GGAGAGACTG GTCTTCCTGG ATTACCAGGA AACCCTGGAG CAAAAGGTCA ACCGGGCCTG 
CCTGGATTCC CAGGAACACC AGGACTTCCT GGACCAAAAG GTATGAATGG TCCTCCTGGT 
AACCCTGGCT TTCCCGGAGA ACCTGGTCCT GTAGGTGCCG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGGCC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGACAAA AGGGTGATGG AGGATCACCT GGACTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AGCCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCTCCT 
GGTTCTCCTG GTCAAGCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCACCAGG TACAGAAGGT CCTCGTGGAC CCCCTGGAAA TGGAGGTATC 
AAAGGAGAGA GGGGAAATCC AGGCCCACCT GGGCAGCCAG GCTTACCTGG TTTGAAAGGA 
GATCAAGGAC CACCAGGACT CCCGGGTAAC CCTGGCCGGC CAGGTCTTAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGAA TGAAAGGACC CATTGGAGTA 
CCAGGCTCCA CTGGCCCTGA TGGGGAACCA GGTCTTACTG GGCCCCCAGG TCCTCCTGGG 
TTGCCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATCCTGGACC TCCAGGGATT 
CCTGGACAAC CTGGATTAAA AGGTCTACCA GGACTTCCAG GCCCTCAAGG CCTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCAGGACGC AATGGACTCC CAGGCTTTGA TGGTGCAGGA 
GGGCGCAAAG GAGACCCAGG GCTGCCAGGA CAGCCAGGTG CCCGTGGATT GGATGGTCCC 
CCAGGACCAG ATGGATTGCA AGGACCTCCA GGACCCCCTG GAACCACCTC TGTTGCCCAT 
GGATTCCTCA TCACACGTCA CAGTCAAACA ACAGAAGCAC CACAATGCCC ACGGGGAACA 
GTTCATATCT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCCCATGGT 
CAAGACTTGG GTACGGCTGG CAGCTGCCTT CGTCGCTTCA GTACCATGCC TTTCATGTTC 
TGCAACATTA ACAACGTTTG TAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTTTCC 
ACCCCAGAGC CCATGCCAAT GAACATGGAA CCCCTGAAGG GACAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTGTG TGAGGCGCCA GCTGTGGTGA TCGCTGTTCA CAGCCAGACT 
ATTCAGATTC CACACTGTCC TCAGGGGTGG GACTCTCTGT GGATTGGCTA TTCCTTCATG 
ATGCATACAA GTGCAGGAGC AGAAGGCTCT GGCCAAGCCC TAGCCTCTCC TGGCTCCTGT 
TTGGAAGAAT TTCGTTCCGC TCCCTTCATC GAATGTCATG GGCGAGGGAC ATGCAATTAC 
TATGCCAATT CGTACAGCTT TTGGCTGGCC ACTGTTGATA TGTCAGACAT GTTCAACAAA 
CCTCAGTCAG AAACCCTGAA AGCAGGAGAC TTGAGGACGC GTATTAGCCG ATGTCAAGTA 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528760.1
CDS	366..5423
Translation	MQVRGVCLAAGWFLLALCLWGQPAEAAACHGCSSGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSPLPGPKGNQGYPGPPGIQGPPGPKGISGPSGPPGPPGLMGPPGPPGPPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDIEFQKGDQGVPGDRGPPGPPGIPGPPGPPGGVKGEKGEPGEAGKRGKPGKDGETGQPGIPGLPGDPGYPGEPGRDGEKGQKGDIGPPGPPGLVIPAPGTGVTMGEKGNIGLPGLPGEKGERGFPGIQGPPGFPGPPGTAVVGPPGPPGYPGERGQKGDEGPPGICIPGSPGLDGQPGAPGLPGPPGPPGPQLPSRDEICKAGPPGPPGPPGDKGLQGERGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGIPGEKGDKGQAGITGPKGLPGIPGPPGAPGFPGSKGDPGDVLTLPGMKGDKGELGFPGAPGLPGLPGSPGKDGLPGLPGPKGEPGGITFKGERGPPGSPGLPGLPGNMGPTGPVGFGPPGPIGEKGIQGVAGNPGQPGLPGPKGDPGQTITQPGKPGLPGSPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGPPGPPGPKGFPGIPGPPGAPGAPGRMGPDGPPGPPGFPGPKGEPGFGLPGPPGPPGFPGFKGTPGPKGDRGFPGPSGPPGRTGLDGLPGPKGDVGPNGQPGPVGPPGLPGIGLQGPPGPPGTPGPIGQPGLHGIPGEKGDPGPPGFDVPGLPGERGSPGLPGAPGLIGPPGSPGVPGKAGVPGFPGVKGEMGMMGPPGPPGPLGIPGRSGAPGLKGDDGMQGQPGLPGPAGEKGGKGEPGLPGPPGPMHPDFLGSKGEKGEPGLPGIPGLSGPKGYQGLPGDPGQPGLSGQPGSPGLPGPKGNPGLPGPPGLTGPPGPKGNIGDMGFPGPQGVDGPPGPPGFPGQPGSPGLPGQKGSKGEPGVSGIGLPGLPGPKGEPGLPGYPGNPGIKGSVGETGLPGLPGNPGAKGQPGLPGFPGTPGLPGPKGMNGPPGNPGFPGEPGPVGAGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDGGSPGLPGNPGLPGSKGEPGFQGFPGMPGPPGPPGSPGQALEGPKGNPGPQGPPGRPGPPGTEGPRGPPGNGGIKGERGNPGPPGQPGLPGLKGDQGPPGLPGNPGRPGLNGMKGDPGLPGVPGFPGMKGPIGVPGSTGPDGEPGLTGPPGPPGLPGPSGQSIVIKGDPGPPGIPGQPGLKGLPGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGARGLDGPPGPDGLQGPPGPPGTTSVAHGFLITRHSQTTEAPQCPRGTVHIYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMNMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDMSDMFNKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006528697.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528697.4

ORF Insert Sequence:

ATGCAAGTGC GTGGAGTGTG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCCG CAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGGTTTCCAG GTTTAGAAGG TCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGCCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGGATGCCA GGCCATGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC GGGCTTTCCT 
GGTTTACAAG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGGGA ACCAGGCAGT 
ATAATCATGT CACCACTGCC AGGACCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGCC CACCTGGTCC CAAGGGTATA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GACCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGC GAACAAGGTC TTCAGGGCCC ACCTGGTCCA 
CCTGGGCAGA TCAGCGAACA GAAAAGACCA ATTGACATAG AATTTCAGAA AGGAGATCAG 
GGAGTTCCTG GTGATAGAGG GCCTCCTGGA CCTCCAGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCAGGAGAGG CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG CCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGAGAAC CAGGAAGAGA TGGTGAAAAG GGTCAAAAGG GTGATATTGG CCCACCTGGA 
CCTCCCGGAC TTGTAATTCC TGCACCTGGA ACTGGTGTGA CTATGGGAGA AAAAGGAAAT 
ATCGGGTTAC CTGGTTTGCC TGGAGAAAAG GGAGAGCGAG GATTTCCTGG AATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGAACA GCAGTTGTGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGCCTTC CAGGACCTCC TGGCCCCCCT 
GGCCCCCAGT TGCCATCCAG AGATGAAATC TGTAAAGCAG GCCCTCCTGG GCCTCCAGGA 
CCTCCAGGTG ATAAAGGACT CCAAGGAGAG CGAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCTGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGGGACAA AGGACAAGCT 
GGGATAACTG GTCCGAAAGG ATTGCCAGGC ATACCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGGGATGTC CTCACTCTTC CAGGAATGAA AGGTGACAAA 
GGAGAGTTGG GTTTCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTCCC TGGCCCCAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CTCCTGGGAG CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCAGTTG GTTTTGGGCC TCCAGGCCCC ATAGGCGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCGGG ATTACCAGGT CCTAAAGGAG ATCCAGGCCA GACCATAACC 
CAGCCAGGAA AGCCTGGTTT GCCAGGTAGT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCTGGA CCACCTGGTC CTAAAGGTTT TCCAGGAATT 
CCGGGACCTC CAGGAGCTCC TGGGGCCCCT GGAAGAATGG GTCCAGATGG GCCTCCTGGG 
CCACCAGGCT TCCCAGGACC AAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTTCAG GTCCTCCAGG ACGCACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGCCCAGTA GGGCCTCCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGG 
ATTCCAGGAG AGAAGGGGGA CCCAGGACCT CCTGGGTTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTGTCCCTG GAAAAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC TGGACCTTTG GGAATTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGATGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGC AGGAGAAAAG 
GGCGGTAAGG GAGAGCCTGG CCTTCCAGGT CCTCCTGGAC CAATGCATCC AGATTTCCTG 
GGATCGAAAG GAGAAAAGGG TGAGCCCGGC TTACCAGGTA TTCCTGGACT TTCAGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCAGGA 
TCACCAGGAC TCCCAGGTCC CAAGGGTAAT CCTGGTCTCC CTGGTCCACC AGGACTTACA 
GGACCTCCTG GACCAAAAGG AAACATTGGT GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCCC CTGGACCTCC TGGATTTCCT GGACAACCTG GCTCTCCTGG GTTACCTGGC 
CAGAAAGGAA GTAAAGGAGA GCCTGGGGTT TCAGGCATTG GACTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGGTCGGTG 
GGAGAGACTG GTCTTCCTGG ATTACCAGGA AACCCTGGAG CAAAAGGTCA ACCGGGCCTG 
CCTGGATTCC CAGGAACACC AGGACTTCCT GGACCAAAAG GTATGAATGG TCCTCCTGGT 
AACCCTGGCT TTCCCGGAGA ACCTGGTCCT GTAGGTGCCG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGGCC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGACAAA AGGGTGATGG AGGATCACCT GGACTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AGCCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCTCCT 
GGTTCTCCTG GTCAAGCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCACCAGG TACAGAAGGT CCTCGTGGAC CCCCTGGAAA TGGAGGTATC 
AAAGGAGAGA GGGGAAATCC AGGCCCACCT GGGCAGCCAG GCTTACCTGG TTTGAAAGGA 
GATCAAGGAC CACCAGGACT CCCGGGTAAC CCTGGCCGGC CAGGTCTTAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGAA TGAAAGGACC CATTGGAGTA 
CCAGGCTCCA CTGGCCCTGA TGGGGAACCA GGTCTTACTG GGCCCCCAGG TCCTCCTGGG 
TTGCCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATCCTGGACC TCCAGGGATT 
CCTGGACAAC CTGGATTAAA AGGTCTACCA GGACTTCCAG GCCCTCAAGG CCTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCAGGACGC AATGGACTCC CAGGCTTTGA TGGTGCAGGA 
GGGCGCAAAG GAGACCCAGG GCTGCCAGGA CAGCCAGGTG CCCGTGGATT GGATGGTCCC 
CCAGGACCAG ATGGATTGCA AGGACCTCCA GGACCCCCTG GAACCACCTC TGTTGCCCAT 
GGATTCCTCA TCACACGTCA CAGTCAAACA ACAGAAGCAC CACAATGCCC ACGGGGAACA 
GTTCATATCT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCCCATGGT 
CAAGACTTGG GTACGGCTGG CAGCTGCCTT CGTCGCTTCA GTACCATGCC TTTCATGTTC 
TGCAACATTA ACAACGTTTG TAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTTTCC 
ACCCCAGAGC CCATGCCAAT GAACATGGAA CCCCTGAAGG GACAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTGTG TGAGGCGCCA GCTGTGGTGA TCGCTGTTCA CAGCCAGACT 
ATTCAGATTC CACACTGTCC TCAGGGGTGG GACTCTCTGT GGATTGGCTA TTCCTTCATG 
ATGCATACAA GTGCAGGAGC AGAAGGCTCT GGCCAAGCCC TAGCCTCTCC TGGCTCCTGT 
TTGGAAGAAT TTCGTTCCGC TCCCTTCATC GAATGTCATG GGCGAGGGAC ATGCAATTAC 
TATGCCAATT CGTACAGCTT TTGGCTGGCC ACTGTTGATA TGTCAGACAT GTTCAACAAA 
CCTCAGTCAG AAACCCTGAA AGCAGGAGAC TTGAGGACGC GTATTAGCCG ATGTCAAGTA 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a5 ( XM_017318372.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173861.1 Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant X3, mRNA.

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CloneID	OMu83066
Clone ID Related Accession (Same CDS sequence)	XM_017318372.2 , XM_017318372.1
Accession Version	XM_017318372.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-5(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318372.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTGC GTGGAGTGTG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCCG CAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGGTTTCCAG GTTTAGAAGG TCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGCCCT CCAGGACCTC GGGGACAAAA GGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGGA TGCCAGGCCA TGATGGGGCT 
CCAGGACCTC AAGGTATCCC TGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCGGGCT TTCCTGGTTT ACAAGGTCCT CCAGGACCTC CTGGGATCCC AGGTATGAAG 
GGGGAACCAG GCAGTATAAT CATGTCACCA CTGCCAGGAC CAAAGGGTAA TCAAGGATAC 
CCAGGTCCTC CTGGAATACA AGGCCCACCT GGTCCCAAGG GTATATCAGG GCCAAGTGGT 
CCTCCAGGAC CACCAGGTTT AATGGGACCT CCTGGCCCAC CAGGCCCACC GGGACCAAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGCGAACA AGGTCTTCAG 
GGCCCACCTG GTCCACCTGG GCAGATCAGC GAACAGAAAA GACCAATTGA CATAGAATTT 
CAGAAAGGAG ATCAGGGAGT TCCTGGTGAT AGAGGGCCTC CTGGACCTCC AGGAATACCT 
GGTCCTCCCG GTCCTCCTGG TGGTGTGAAA GGTGAAAAGG GTGAGCCAGG AGAGGCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAG ACTGGCCAAC CAGGAATCCC AGGTTTGCCT 
GGTGATCCTG GTTACCCTGG AGAACCAGGA AGAGATGGTG AAAAGGGTCA AAAGGGTGAT 
ATTGGCCCAC CTGGACCTCC CGGACTTGTA ATTCCTGCAC CTGGAACTGG TGTGACTATG 
GGAGAAAAAG GAAATATCGG GTTACCTGGT TTGCCTGGAG AAAAGGGAGA GCGAGGATTT 
CCTGGAATAC AAGGTCCACC TGGCTTTCCT GGACCTCCAG GAACAGCAGT TGTGGGTCCC 
CCTGGTCCTC CTGGATATCC TGGTGAAAGG GGCCAGAAAG GTGATGAAGG TCCCCCTGGA 
ATTTGTATTC CTGGATCTCC TGGACTTGAT GGACAGCCTG GGGCTCCTGG CCTTCCAGGA 
CCTCCTGGCC CCCCTGGCCC CCAGTTGCCA TCCAGAGATG AAATCTGTAA AGCAGGCCCT 
CCTGGGCCTC CAGGACCTCC AGGTGATAAA GGACTCCAAG GAGAGCGAGG AGTAAAAGGT 
GACAAAGGTG ATACTTGCTT CAACTGTATT GGAACTGGCA TTTCAGGGCC TCCAGGCCAA 
CCTGGTTTAC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GAATCCCTGG AGAGAAGGGG 
GACAAAGGAC AAGCTGGGAT AACTGGTCCG AAAGGATTGC CAGGCATACC TGGACCTCCA 
GGTGCTCCAG GCTTTCCAGG GTCTAAGGGT GACCCTGGGG ATGTCCTCAC TCTTCCAGGA 
ATGAAAGGTG ACAAAGGAGA GTTGGGTTTC CCTGGAGCTC CAGGGCTTCC TGGTCTACCT 
GGTAGTCCTG GAAAGGATGG TTTGCCAGGA CTCCCTGGCC CCAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCTCCT GGGAGCCCAG GTTTACCAGG TCTCCCAGGA 
AATATGGGAC CTACAGGCCC AGTTGGTTTT GGGCCTCCAG GCCCCATAGG CGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCGGGATTAC CAGGTCCTAA AGGAGATCCA 
GGCCAGACCA TAACCCAGCC AGGAAAGCCT GGTTTGCCAG GTAGTCCAGG CAGAGATGGT 
GAAGTGGGTC TTCCAGGTGA TCCTGGACTT CCAGGCCAAC CAGGCCTGCC AGGAATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGG ATTGGGCCTC CTGGACCACC TGGTCCTAAA 
GGTTTTCCAG GAATTCCGGG ACCTCCAGGA GCTCCTGGGG CCCCTGGAAG AATGGGTCCA 
GATGGGCCTC CTGGGCCACC AGGCTTCCCA GGACCAAAGG GAGAGCCAGG ATTTGGGTTA 
CCTGGGCCAC CTGGGCCACC AGGATTCCCA GGTTTCAAAG GAACACCTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TTCAGGTCCT CCAGGACGCA CTGGCTTGGA TGGGCTACCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGCC CAGTAGGGCC TCCAGGGCTG 
CCAGGAATTG GTCTTCAGGG ACCACCAGGA CCACCAGGGA CTCCAGGGCC AATAGGCCAA 
CCTGGCTTAC ATGGGATTCC AGGAGAGAAG GGGGACCCAG GACCTCCTGG GTTTGATGTT 
CCAGGACTCC CTGGAGAGAG AGGTAGTCCA GGGCTCCCTG GAGCCCCTGG TCTTATAGGA 
CCTCCAGGAT CACCTGGTGT CCCTGGAAAA GCAGGTGTCC CTGGATTTCC AGGTGTCAAA 
GGCGAAATGG GTATGATGGG ACCTCCAGGC CCACCTGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGCTC CTGGTCTTAA AGGTGATGAT GGCATGCAAG GTCAGCCAGG TCTCCCTGGC 
CCTGCAGGAG AAAAGGGCGG TAAGGGAGAG CCTGGCCTTC CAGGTCCTCC TGGACCAATG 
CATCCAGATT TCCTGGGATC GAAAGGAGAA AAGGGTGAGC CCGGCTTACC AGGTATTCCT 
GGACTTTCAG GACCAAAAGG CTACCAGGGA TTACCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CAGGATCACC AGGACTCCCA GGTCCCAAGG GTAATCCTGG TCTCCCTGGT 
CCACCAGGAC TTACAGGACC TCCTGGACCA AAAGGAAACA TTGGTGACAT GGGTTTTCCT 
GGCCCTCAAG GTGTAGATGG GCCCCCTGGA CCTCCTGGAT TTCCTGGACA ACCTGGCTCT 
CCTGGGTTAC CTGGCCAGAA AGGAAGTAAA GGAGAGCCTG GGGTTTCAGG CATTGGACTT 
CCAGGTCTTC CTGGCCCAAA GGGTGAACCT GGTCTGCCTG GATATCCAGG AAACCCAGGT 
ATCAAAGGGT CGGTGGGAGA GACTGGTCTT CCTGGATTAC CAGGAAACCC TGGAGCAAAA 
GGTCAACCGG GCCTGCCTGG ATTCCCAGGA ACACCAGGAC TTCCTGGACC AAAAGGTATG 
AATGGTCCTC CTGGTAACCC TGGCTTTCCC GGAGAACCTG GTCCTGTAGG TGCCGGAGGG 
CGTCCTGGAC CACCAGGACC TCCTGGTGAA AAAGGCAAGC CAGGTCAAGA TGGCATTCCT 
GGGCCAGCAG GACAAAAGGG AGAACCAGGT CAACCAGGCT TTGGAATCCC AGGACCTCCT 
GGACTCCCAG GACTTTCTGG ACAAAAGGGT GATGGAGGAT CACCTGGACT TCCAGGAAAT 
CCTGGCCTTC CAGGTTCAAA GGGTGAGCCA GGCTTTCAGG GATTCCCTGG TATGCCAGGT 
CCCCCAGGTC CTCCTGGTTC TCCTGGTCAA GCTTTGGAAG GTCCCAAAGG AAACCCTGGG 
CCTCAAGGCC CTCCTGGGAG ACCAGGTCCT CCAGGTTTTC AAGGTCCACC AGGTACAGAA 
GGTCCTCGTG GACCCCCTGG AAATGGAGGT ATCAAAGGAG AGAGGGGAAA TCCAGGCCCA 
CCTGGGCAGC CAGGCTTACC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCCGGGT 
AACCCTGGCC GGCCAGGTCT TAATGGAATG AAAGGAGATC CTGGTCTCCC TGGTGTTCCA 
GGATTCCCAG GAATGAAAGG ACCCATTGGA GTACCAGGCT CCACTGGCCC TGATGGGGAA 
CCAGGTCTTA CTGGGCCCCC AGGTCCTCCT GGGTTGCCTG GTCCTTCAGG ACAGAGTATT 
GTAATCAAAG GAGATCCTGG ACCTCCAGGG ATTCCTGGAC AACCTGGATT AAAAGGTCTA 
CCAGGACTTC CAGGCCCTCA AGGCCTACCA GGTCCAATTG GCCCTCCAGG AGATCCAGGA 
CGCAATGGAC TCCCAGGCTT TGATGGTGCA GGAGGGCGCA AAGGAGACCC AGGGCTGCCA 
GGACAGCCAG GTGCCCGTGG ATTGGATGGT CCCCCAGGAC CAGATGGATT GCAAGGACCT 
CCAGGACCCC CTGGAACCAC CTCTGTTGCC CATGGATTCC TCATCACACG TCACAGTCAA 
ACAACAGAAG CACCACAATG CCCACGGGGA ACAGTTCATA TCTATGAAGG CTTTTCTCTC 
CTGTATGTAC AAGGAAATAA AAGAGCCCAT GGTCAAGACT TGGGTACGGC TGGCAGCTGC 
CTTCGTCGCT TCAGTACCAT GCCTTTCATG TTCTGCAACA TTAACAACGT TTGTAACTTT 
GCTTCAAGAA ATGACTATTC TTACTGGCTT TCCACCCCAG AGCCCATGCC AATGAACATG 
GAACCCCTGA AGGGACAGAG CATCCAGCCA TTCATTAGTC GATGTGCAGT GTGTGAGGCG 
CCAGCTGTGG TGATCGCTGT TCACAGCCAG ACTATTCAGA TTCCACACTG TCCTCAGGGG 
TGGGACTCTC TGTGGATTGG CTATTCCTTC ATGATGCATA CAAGTGCAGG AGCAGAAGGC 
TCTGGCCAAG CCCTAGCCTC TCCTGGCTCC TGTTTGGAAG AATTTCGTTC CGCTCCCTTC 
ATCGAATGTC ATGGGCGAGG GACATGCAAT TACTATGCCA ATTCGTACAG CTTTTGGCTG 
GCCACTGTTG ATATGTCAGA CATGTTCAAC AAACCTCAGT CAGAAACCCT GAAAGCAGGA 
GACTTGAGGA CGCGTATTAG CCGATGTCAA GTATGCATGA AGAGGACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173861.1
CDS	366..5396
Translation	MQVRGVCLAAGWFLLALCLWGQPAEAAACHGCSSGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSPLPGPKGNQGYPGPPGIQGPPGPKGISGPSGPPGPPGLMGPPGPPGPPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDIEFQKGDQGVPGDRGPPGPPGIPGPPGPPGGVKGEKGEPGEAGKRGKPGKDGETGQPGIPGLPGDPGYPGEPGRDGEKGQKGDIGPPGPPGLVIPAPGTGVTMGEKGNIGLPGLPGEKGERGFPGIQGPPGFPGPPGTAVVGPPGPPGYPGERGQKGDEGPPGICIPGSPGLDGQPGAPGLPGPPGPPGPQLPSRDEICKAGPPGPPGPPGDKGLQGERGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGIPGEKGDKGQAGITGPKGLPGIPGPPGAPGFPGSKGDPGDVLTLPGMKGDKGELGFPGAPGLPGLPGSPGKDGLPGLPGPKGEPGGITFKGERGPPGSPGLPGLPGNMGPTGPVGFGPPGPIGEKGIQGVAGNPGQPGLPGPKGDPGQTITQPGKPGLPGSPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGPPGPPGPKGFPGIPGPPGAPGAPGRMGPDGPPGPPGFPGPKGEPGFGLPGPPGPPGFPGFKGTPGPKGDRGFPGPSGPPGRTGLDGLPGPKGDVGPNGQPGPVGPPGLPGIGLQGPPGPPGTPGPIGQPGLHGIPGEKGDPGPPGFDVPGLPGERGSPGLPGAPGLIGPPGSPGVPGKAGVPGFPGVKGEMGMMGPPGPPGPLGIPGRSGAPGLKGDDGMQGQPGLPGPAGEKGGKGEPGLPGPPGPMHPDFLGSKGEKGEPGLPGIPGLSGPKGYQGLPGDPGQPGLSGQPGSPGLPGPKGNPGLPGPPGLTGPPGPKGNIGDMGFPGPQGVDGPPGPPGFPGQPGSPGLPGQKGSKGEPGVSGIGLPGLPGPKGEPGLPGYPGNPGIKGSVGETGLPGLPGNPGAKGQPGLPGFPGTPGLPGPKGMNGPPGNPGFPGEPGPVGAGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDGGSPGLPGNPGLPGSKGEPGFQGFPGMPGPPGPPGSPGQALEGPKGNPGPQGPPGRPGPPGFQGPPGTEGPRGPPGNGGIKGERGNPGPPGQPGLPGLKGDQGPPGLPGNPGRPGLNGMKGDPGLPGVPGFPGMKGPIGVPGSTGPDGEPGLTGPPGPPGLPGPSGQSIVIKGDPGPPGIPGQPGLKGLPGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGARGLDGPPGPDGLQGPPGPPGTTSVAHGFLITRHSQTTEAPQCPRGTVHIYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMNMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDMSDMFNKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_017318372.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318372.2

ORF Insert Sequence:

ATGCAAGTGC GTGGAGTGTG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCCG CAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGGTTTCCAG GTTTAGAAGG TCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGCCCT CCAGGACCTC GGGGACAAAA GGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGGA TGCCAGGCCA TGATGGGGCT 
CCAGGACCTC AAGGTATCCC TGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCGGGCT TTCCTGGTTT ACAAGGTCCT CCAGGACCTC CTGGGATCCC AGGTATGAAG 
GGGGAACCAG GCAGTATAAT CATGTCACCA CTGCCAGGAC CAAAGGGTAA TCAAGGATAC 
CCAGGTCCTC CTGGAATACA AGGCCCACCT GGTCCCAAGG GTATATCAGG GCCAAGTGGT 
CCTCCAGGAC CACCAGGTTT AATGGGACCT CCTGGCCCAC CAGGCCCACC GGGACCAAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGCGAACA AGGTCTTCAG 
GGCCCACCTG GTCCACCTGG GCAGATCAGC GAACAGAAAA GACCAATTGA CATAGAATTT 
CAGAAAGGAG ATCAGGGAGT TCCTGGTGAT AGAGGGCCTC CTGGACCTCC AGGAATACCT 
GGTCCTCCCG GTCCTCCTGG TGGTGTGAAA GGTGAAAAGG GTGAGCCAGG AGAGGCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAG ACTGGCCAAC CAGGAATCCC AGGTTTGCCT 
GGTGATCCTG GTTACCCTGG AGAACCAGGA AGAGATGGTG AAAAGGGTCA AAAGGGTGAT 
ATTGGCCCAC CTGGACCTCC CGGACTTGTA ATTCCTGCAC CTGGAACTGG TGTGACTATG 
GGAGAAAAAG GAAATATCGG GTTACCTGGT TTGCCTGGAG AAAAGGGAGA GCGAGGATTT 
CCTGGAATAC AAGGTCCACC TGGCTTTCCT GGACCTCCAG GAACAGCAGT TGTGGGTCCC 
CCTGGTCCTC CTGGATATCC TGGTGAAAGG GGCCAGAAAG GTGATGAAGG TCCCCCTGGA 
ATTTGTATTC CTGGATCTCC TGGACTTGAT GGACAGCCTG GGGCTCCTGG CCTTCCAGGA 
CCTCCTGGCC CCCCTGGCCC CCAGTTGCCA TCCAGAGATG AAATCTGTAA AGCAGGCCCT 
CCTGGGCCTC CAGGACCTCC AGGTGATAAA GGACTCCAAG GAGAGCGAGG AGTAAAAGGT 
GACAAAGGTG ATACTTGCTT CAACTGTATT GGAACTGGCA TTTCAGGGCC TCCAGGCCAA 
CCTGGTTTAC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GAATCCCTGG AGAGAAGGGG 
GACAAAGGAC AAGCTGGGAT AACTGGTCCG AAAGGATTGC CAGGCATACC TGGACCTCCA 
GGTGCTCCAG GCTTTCCAGG GTCTAAGGGT GACCCTGGGG ATGTCCTCAC TCTTCCAGGA 
ATGAAAGGTG ACAAAGGAGA GTTGGGTTTC CCTGGAGCTC CAGGGCTTCC TGGTCTACCT 
GGTAGTCCTG GAAAGGATGG TTTGCCAGGA CTCCCTGGCC CCAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCTCCT GGGAGCCCAG GTTTACCAGG TCTCCCAGGA 
AATATGGGAC CTACAGGCCC AGTTGGTTTT GGGCCTCCAG GCCCCATAGG CGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCGGGATTAC CAGGTCCTAA AGGAGATCCA 
GGCCAGACCA TAACCCAGCC AGGAAAGCCT GGTTTGCCAG GTAGTCCAGG CAGAGATGGT 
GAAGTGGGTC TTCCAGGTGA TCCTGGACTT CCAGGCCAAC CAGGCCTGCC AGGAATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGG ATTGGGCCTC CTGGACCACC TGGTCCTAAA 
GGTTTTCCAG GAATTCCGGG ACCTCCAGGA GCTCCTGGGG CCCCTGGAAG AATGGGTCCA 
GATGGGCCTC CTGGGCCACC AGGCTTCCCA GGACCAAAGG GAGAGCCAGG ATTTGGGTTA 
CCTGGGCCAC CTGGGCCACC AGGATTCCCA GGTTTCAAAG GAACACCTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TTCAGGTCCT CCAGGACGCA CTGGCTTGGA TGGGCTACCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGCC CAGTAGGGCC TCCAGGGCTG 
CCAGGAATTG GTCTTCAGGG ACCACCAGGA CCACCAGGGA CTCCAGGGCC AATAGGCCAA 
CCTGGCTTAC ATGGGATTCC AGGAGAGAAG GGGGACCCAG GACCTCCTGG GTTTGATGTT 
CCAGGACTCC CTGGAGAGAG AGGTAGTCCA GGGCTCCCTG GAGCCCCTGG TCTTATAGGA 
CCTCCAGGAT CACCTGGTGT CCCTGGAAAA GCAGGTGTCC CTGGATTTCC AGGTGTCAAA 
GGCGAAATGG GTATGATGGG ACCTCCAGGC CCACCTGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGCTC CTGGTCTTAA AGGTGATGAT GGCATGCAAG GTCAGCCAGG TCTCCCTGGC 
CCTGCAGGAG AAAAGGGCGG TAAGGGAGAG CCTGGCCTTC CAGGTCCTCC TGGACCAATG 
CATCCAGATT TCCTGGGATC GAAAGGAGAA AAGGGTGAGC CCGGCTTACC AGGTATTCCT 
GGACTTTCAG GACCAAAAGG CTACCAGGGA TTACCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CAGGATCACC AGGACTCCCA GGTCCCAAGG GTAATCCTGG TCTCCCTGGT 
CCACCAGGAC TTACAGGACC TCCTGGACCA AAAGGAAACA TTGGTGACAT GGGTTTTCCT 
GGCCCTCAAG GTGTAGATGG GCCCCCTGGA CCTCCTGGAT TTCCTGGACA ACCTGGCTCT 
CCTGGGTTAC CTGGCCAGAA AGGAAGTAAA GGAGAGCCTG GGGTTTCAGG CATTGGACTT 
CCAGGTCTTC CTGGCCCAAA GGGTGAACCT GGTCTGCCTG GATATCCAGG AAACCCAGGT 
ATCAAAGGGT CGGTGGGAGA GACTGGTCTT CCTGGATTAC CAGGAAACCC TGGAGCAAAA 
GGTCAACCGG GCCTGCCTGG ATTCCCAGGA ACACCAGGAC TTCCTGGACC AAAAGGTATG 
AATGGTCCTC CTGGTAACCC TGGCTTTCCC GGAGAACCTG GTCCTGTAGG TGCCGGAGGG 
CGTCCTGGAC CACCAGGACC TCCTGGTGAA AAAGGCAAGC CAGGTCAAGA TGGCATTCCT 
GGGCCAGCAG GACAAAAGGG AGAACCAGGT CAACCAGGCT TTGGAATCCC AGGACCTCCT 
GGACTCCCAG GACTTTCTGG ACAAAAGGGT GATGGAGGAT CACCTGGACT TCCAGGAAAT 
CCTGGCCTTC CAGGTTCAAA GGGTGAGCCA GGCTTTCAGG GATTCCCTGG TATGCCAGGT 
CCCCCAGGTC CTCCTGGTTC TCCTGGTCAA GCTTTGGAAG GTCCCAAAGG AAACCCTGGG 
CCTCAAGGCC CTCCTGGGAG ACCAGGTCCT CCAGGTTTTC AAGGTCCACC AGGTACAGAA 
GGTCCTCGTG GACCCCCTGG AAATGGAGGT ATCAAAGGAG AGAGGGGAAA TCCAGGCCCA 
CCTGGGCAGC CAGGCTTACC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCCGGGT 
AACCCTGGCC GGCCAGGTCT TAATGGAATG AAAGGAGATC CTGGTCTCCC TGGTGTTCCA 
GGATTCCCAG GAATGAAAGG ACCCATTGGA GTACCAGGCT CCACTGGCCC TGATGGGGAA 
CCAGGTCTTA CTGGGCCCCC AGGTCCTCCT GGGTTGCCTG GTCCTTCAGG ACAGAGTATT 
GTAATCAAAG GAGATCCTGG ACCTCCAGGG ATTCCTGGAC AACCTGGATT AAAAGGTCTA 
CCAGGACTTC CAGGCCCTCA AGGCCTACCA GGTCCAATTG GCCCTCCAGG AGATCCAGGA 
CGCAATGGAC TCCCAGGCTT TGATGGTGCA GGAGGGCGCA AAGGAGACCC AGGGCTGCCA 
GGACAGCCAG GTGCCCGTGG ATTGGATGGT CCCCCAGGAC CAGATGGATT GCAAGGACCT 
CCAGGACCCC CTGGAACCAC CTCTGTTGCC CATGGATTCC TCATCACACG TCACAGTCAA 
ACAACAGAAG CACCACAATG CCCACGGGGA ACAGTTCATA TCTATGAAGG CTTTTCTCTC 
CTGTATGTAC AAGGAAATAA AAGAGCCCAT GGTCAAGACT TGGGTACGGC TGGCAGCTGC 
CTTCGTCGCT TCAGTACCAT GCCTTTCATG TTCTGCAACA TTAACAACGT TTGTAACTTT 
GCTTCAAGAA ATGACTATTC TTACTGGCTT TCCACCCCAG AGCCCATGCC AATGAACATG 
GAACCCCTGA AGGGACAGAG CATCCAGCCA TTCATTAGTC GATGTGCAGT GTGTGAGGCG 
CCAGCTGTGG TGATCGCTGT TCACAGCCAG ACTATTCAGA TTCCACACTG TCCTCAGGGG 
TGGGACTCTC TGTGGATTGG CTATTCCTTC ATGATGCATA CAAGTGCAGG AGCAGAAGGC 
TCTGGCCAAG CCCTAGCCTC TCCTGGCTCC TGTTTGGAAG AATTTCGTTC CGCTCCCTTC 
ATCGAATGTC ATGGGCGAGG GACATGCAAT TACTATGCCA ATTCGTACAG CTTTTGGCTG 
GCCACTGTTG ATATGTCAGA CATGTTCAAC AAACCTCAGT CAGAAACCCT GAAAGCAGGA 
GACTTGAGGA CGCGTATTAG CCGATGTCAA GTATGCATGA AGAGGACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a5 ( NM_007736.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031762.2 Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu00702
Clone ID Related Accession (Same CDS sequence)	NM_007736.4 , NM_001163155.1
Accession Version	NM_007736.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	collagen alpha-5(IV) chain precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK133179.1 and BX571779.6. On Jul 10, 2009 this sequence version replaced NM_007736.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK133179.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAAGTGC GTGGAGTGTG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCCG CAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGGTTTCCAG GTTTAGAAGG TCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGCCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGGATGCCA GGCCATGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC GGGCTTTCCT 
GGTTTACAAG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGGGA ACCAGGCAGT 
ATAATCATGT CACCACTGCC AGGACCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGCC CACCTGGTCC CAAGGGTATA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GACCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGC GAACAAGGTC TTCAGGGCCC ACCTGGTCCA 
CCTGGGCAGA TCAGCGAACA GAAAAGACCA ATTGACATAG AATTTCAGAA AGGAGATCAG 
GGAGTTCCTG GTGATAGAGG GCCTCCTGGA CCTCCAGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCAGGAGAGG CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG CCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGAGAAC CAGGAAGAGA TGGTGAAAAG GGTCAAAAGG GTGATATTGG CCCACCTGGA 
CCTCCCGGAC TTGTAATTCC TGCACCTGGA ACTGGTGTGA CTATGGGAGA AAAAGGAAAT 
ATCGGGTTAC CTGGTTTGCC TGGAGAAAAG GGAGAGCGAG GATTTCCTGG AATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGAACA GCAGTTGTGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGCCTTC CAGGACCTCC TGGCCCCCCT 
GGCCCCCAGT TGCCATCCAG AGATGAAATC TGTAAAGCAG GCCCTCCTGG GCCTCCAGGA 
CCTCCAGGTG ATAAAGGACT CCAAGGAGAG CGAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCTGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGGGACAA AGGACAAGCT 
GGGATAACTG GTCCGAAAGG ATTGCCAGGC ATACCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGGGATGTC CTCACTCTTC CAGGAATGAA AGGTGACAAA 
GGAGAGTTGG GTTTCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTCCC TGGCCCCAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CTCCTGGGAG CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCAGTTG GTTTTGGGCC TCCAGGCCCC ATAGGCGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCGGG ATTACCAGGT CCTAAAGGAG ATCCAGGCCA GACCATAACC 
CAGCCAGGAA AGCCTGGTTT GCCAGGTAGT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCTGGA CCACCTGGTC CTAAAGGTTT TCCAGGAATT 
CCGGGACCTC CAGGAGCTCC TGGGGCCCCT GGAAGAATGG GTCCAGATGG GCCTCCTGGG 
CCACCAGGCT TCCCAGGACC AAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTTCAG GTCCTCCAGG ACGCACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGCCCAGTA GGGCCTCCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGG 
ATTCCAGGAG AGAAGGGGGA CCCAGGACCT CCTGGGTTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTGTCCCTG GAAAAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC TGGACCTTTG GGAATTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGATGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGC AGGAGAAAAG 
GGCGGTAAGG GAGAGCCTGG CCTTCCAGGT CCTCCTGGAC CAATGCATCC AGATTTCCTG 
GGATCGAAAG GAGAAAAGGG TGAGCCCGGC TTACCAGGTA TTCCTGGACT TTCAGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCAGGA 
TCACCAGGAC TCCCAGGTCC CAAGGGTAAT CCTGGTCTCC CTGGTCCACC AGGACTTACA 
GGACCTCCTG GACCAAAAGG AAACATTGGT GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCCC CTGGACCTCC TGGATTTCCT GGACAACCTG GCTCTCCTGG GTTACCTGGC 
CAGAAAGGAA GTAAAGGAGA GCCTGGGGTT TCAGGCATTG GACTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGGTCGGTG 
GGAGAGACTG GTCTTCCTGG ATTACCAGGA AACCCTGGAG CAAAAGGTCA ACCGGGCCTG 
CCTGGATTCC CAGGAACACC AGGACTTCCT GGACCAAAAG GTATGAATGG TCCTCCTGGT 
AACCCTGGCT TTCCCGGAGA ACCTGGTCCT GTAGGTGCCG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGGCC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGACAAA AGGGTGATGG AGGATCACCT GGACTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AGCCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCTCCT 
GGTTCTCCTG GTCAAGCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCTCCAGG TTTTCAAGGT CCACCAGGTA CAGAAGGTCC TCGTGGACCC 
CCTGGAAATG GAGGTATCAA AGGAGAGAGG GGAAATCCAG GCCCACCTGG GCAGCCAGGC 
TTACCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC CGGGTAACCC TGGCCGGCCA 
GGTCTTAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGAATG 
AAAGGACCCA TTGGAGTACC AGGCTCCACT GGCCCTGATG GGGAACCAGG TCTTACTGGG 
CCCCCAGGTC CTCCTGGGTT GCCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT 
CCTGGACCTC CAGGGATTCC TGGACAACCT GGATTAAAAG GTCTACCAGG ACTTCCAGGC 
CCTCAAGGCC TACCAGGTCC AATTGGCCCT CCAGGAGATC CAGGACGCAA TGGACTCCCA 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGGC TGCCAGGACA GCCAGGTGCC 
CGTGGATTGG ATGGTCCCCC AGGACCAGAT GGATTGCAAG GACCTCCAGG ACCCCCTGGA 
ACCACCTCTG TTGCCCATGG ATTCCTCATC ACACGTCACA GTCAAACAAC AGAAGCACCA 
CAATGCCCAC GGGGAACAGT TCATATCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CCCATGGTCA AGACTTGGGT ACGGCTGGCA GCTGCCTTCG TCGCTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATTAAC AACGTTTGTA ACTTTGCTTC AAGAAATGAC 
TATTCTTACT GGCTTTCCAC CCCAGAGCCC ATGCCAATGA ACATGGAACC CCTGAAGGGA 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTGTGTG AGGCGCCAGC TGTGGTGATC 
GCTGTTCACA GCCAGACTAT TCAGATTCCA CACTGTCCTC AGGGGTGGGA CTCTCTGTGG 
ATTGGCTATT CCTTCATGAT GCATACAAGT GCAGGAGCAG AAGGCTCTGG CCAAGCCCTA 
GCCTCTCCTG GCTCCTGTTT GGAAGAATTT CGTTCCGCTC CCTTCATCGA ATGTCATGGG 
CGAGGGACAT GCAATTACTA TGCCAATTCG TACAGCTTTT GGCTGGCCAC TGTTGATATG 
TCAGACATGT TCAACAAACC TCAGTCAGAA ACCCTGAAAG CAGGAGACTT GAGGACGCGT 
ATTAGCCGAT GTCAAGTATG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031762.2
CDS	145..5220
Translation	MQVRGVCLAAGWFLLALCLWGQPAEAAACHGCSSGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSPLPGPKGNQGYPGPPGIQGPPGPKGISGPSGPPGPPGLMGPPGPPGPPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDIEFQKGDQGVPGDRGPPGPPGIPGPPGPPGGVKGEKGEPGEAGKRGKPGKDGETGQPGIPGLPGDPGYPGEPGRDGEKGQKGDIGPPGPPGLVIPAPGTGVTMGEKGNIGLPGLPGEKGERGFPGIQGPPGFPGPPGTAVVGPPGPPGYPGERGQKGDEGPPGICIPGSPGLDGQPGAPGLPGPPGPPGPQLPSRDEICKAGPPGPPGPPGDKGLQGERGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGIPGEKGDKGQAGITGPKGLPGIPGPPGAPGFPGSKGDPGDVLTLPGMKGDKGELGFPGAPGLPGLPGSPGKDGLPGLPGPKGEPGGITFKGERGPPGSPGLPGLPGNMGPTGPVGFGPPGPIGEKGIQGVAGNPGQPGLPGPKGDPGQTITQPGKPGLPGSPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGPPGPPGPKGFPGIPGPPGAPGAPGRMGPDGPPGPPGFPGPKGEPGFGLPGPPGPPGFPGFKGTPGPKGDRGFPGPSGPPGRTGLDGLPGPKGDVGPNGQPGPVGPPGLPGIGLQGPPGPPGTPGPIGQPGLHGIPGEKGDPGPPGFDVPGLPGERGSPGLPGAPGLIGPPGSPGVPGKAGVPGFPGVKGEMGMMGPPGPPGPLGIPGRSGAPGLKGDDGMQGQPGLPGPAGEKGGKGEPGLPGPPGPMHPDFLGSKGEKGEPGLPGIPGLSGPKGYQGLPGDPGQPGLSGQPGSPGLPGPKGNPGLPGPPGLTGPPGPKGNIGDMGFPGPQGVDGPPGPPGFPGQPGSPGLPGQKGSKGEPGVSGIGLPGLPGPKGEPGLPGYPGNPGIKGSVGETGLPGLPGNPGAKGQPGLPGFPGTPGLPGPKGMNGPPGNPGFPGEPGPVGAGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDGGSPGLPGNPGLPGSKGEPGFQGFPGMPGPPGPPGSPGQALEGPKGNPGPQGPPGRPGPPGFQGPPGTEGPRGPPGNGGIKGERGNPGPPGQPGLPGLKGDQGPPGLPGNPGRPGLNGMKGDPGLPGVPGFPGMKGPIGVPGSTGPDGEPGLTGPPGPPGLPGPSGQSIVIKGDPGPPGIPGQPGLKGLPGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGARGLDGPPGPDGLQGPPGPPGTTSVAHGFLITRHSQTTEAPQCPRGTVHIYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMNMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDMSDMFNKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	NM_007736.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007736.4

ORF Insert Sequence:

ATGCAAGTGC GTGGAGTGTG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCCG CAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGGTTTCCAG GTTTAGAAGG TCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGCCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGGATGCCA GGCCATGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC GGGCTTTCCT 
GGTTTACAAG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGGGA ACCAGGCAGT 
ATAATCATGT CACCACTGCC AGGACCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGCC CACCTGGTCC CAAGGGTATA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GACCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGC GAACAAGGTC TTCAGGGCCC ACCTGGTCCA 
CCTGGGCAGA TCAGCGAACA GAAAAGACCA ATTGACATAG AATTTCAGAA AGGAGATCAG 
GGAGTTCCTG GTGATAGAGG GCCTCCTGGA CCTCCAGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCAGGAGAGG CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG CCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGAGAAC CAGGAAGAGA TGGTGAAAAG GGTCAAAAGG GTGATATTGG CCCACCTGGA 
CCTCCCGGAC TTGTAATTCC TGCACCTGGA ACTGGTGTGA CTATGGGAGA AAAAGGAAAT 
ATCGGGTTAC CTGGTTTGCC TGGAGAAAAG GGAGAGCGAG GATTTCCTGG AATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGAACA GCAGTTGTGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGCCTTC CAGGACCTCC TGGCCCCCCT 
GGCCCCCAGT TGCCATCCAG AGATGAAATC TGTAAAGCAG GCCCTCCTGG GCCTCCAGGA 
CCTCCAGGTG ATAAAGGACT CCAAGGAGAG CGAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCTGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGGGACAA AGGACAAGCT 
GGGATAACTG GTCCGAAAGG ATTGCCAGGC ATACCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGGGATGTC CTCACTCTTC CAGGAATGAA AGGTGACAAA 
GGAGAGTTGG GTTTCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTCCC TGGCCCCAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CTCCTGGGAG CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCAGTTG GTTTTGGGCC TCCAGGCCCC ATAGGCGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCGGG ATTACCAGGT CCTAAAGGAG ATCCAGGCCA GACCATAACC 
CAGCCAGGAA AGCCTGGTTT GCCAGGTAGT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCTGGA CCACCTGGTC CTAAAGGTTT TCCAGGAATT 
CCGGGACCTC CAGGAGCTCC TGGGGCCCCT GGAAGAATGG GTCCAGATGG GCCTCCTGGG 
CCACCAGGCT TCCCAGGACC AAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTTCAG GTCCTCCAGG ACGCACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGCCCAGTA GGGCCTCCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGG 
ATTCCAGGAG AGAAGGGGGA CCCAGGACCT CCTGGGTTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTGTCCCTG GAAAAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC TGGACCTTTG GGAATTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGATGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGC AGGAGAAAAG 
GGCGGTAAGG GAGAGCCTGG CCTTCCAGGT CCTCCTGGAC CAATGCATCC AGATTTCCTG 
GGATCGAAAG GAGAAAAGGG TGAGCCCGGC TTACCAGGTA TTCCTGGACT TTCAGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCAGGA 
TCACCAGGAC TCCCAGGTCC CAAGGGTAAT CCTGGTCTCC CTGGTCCACC AGGACTTACA 
GGACCTCCTG GACCAAAAGG AAACATTGGT GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCCC CTGGACCTCC TGGATTTCCT GGACAACCTG GCTCTCCTGG GTTACCTGGC 
CAGAAAGGAA GTAAAGGAGA GCCTGGGGTT TCAGGCATTG GACTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGGTCGGTG 
GGAGAGACTG GTCTTCCTGG ATTACCAGGA AACCCTGGAG CAAAAGGTCA ACCGGGCCTG 
CCTGGATTCC CAGGAACACC AGGACTTCCT GGACCAAAAG GTATGAATGG TCCTCCTGGT 
AACCCTGGCT TTCCCGGAGA ACCTGGTCCT GTAGGTGCCG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGGCC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGACAAA AGGGTGATGG AGGATCACCT GGACTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AGCCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCTCCT 
GGTTCTCCTG GTCAAGCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCTCCAGG TTTTCAAGGT CCACCAGGTA CAGAAGGTCC TCGTGGACCC 
CCTGGAAATG GAGGTATCAA AGGAGAGAGG GGAAATCCAG GCCCACCTGG GCAGCCAGGC 
TTACCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC CGGGTAACCC TGGCCGGCCA 
GGTCTTAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGAATG 
AAAGGACCCA TTGGAGTACC AGGCTCCACT GGCCCTGATG GGGAACCAGG TCTTACTGGG 
CCCCCAGGTC CTCCTGGGTT GCCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT 
CCTGGACCTC CAGGGATTCC TGGACAACCT GGATTAAAAG GTCTACCAGG ACTTCCAGGC 
CCTCAAGGCC TACCAGGTCC AATTGGCCCT CCAGGAGATC CAGGACGCAA TGGACTCCCA 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGGC TGCCAGGACA GCCAGGTGCC 
CGTGGATTGG ATGGTCCCCC AGGACCAGAT GGATTGCAAG GACCTCCAGG ACCCCCTGGA 
ACCACCTCTG TTGCCCATGG ATTCCTCATC ACACGTCACA GTCAAACAAC AGAAGCACCA 
CAATGCCCAC GGGGAACAGT TCATATCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CCCATGGTCA AGACTTGGGT ACGGCTGGCA GCTGCCTTCG TCGCTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATTAAC AACGTTTGTA ACTTTGCTTC AAGAAATGAC 
TATTCTTACT GGCTTTCCAC CCCAGAGCCC ATGCCAATGA ACATGGAACC CCTGAAGGGA 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTGTGTG AGGCGCCAGC TGTGGTGATC 
GCTGTTCACA GCCAGACTAT TCAGATTCCA CACTGTCCTC AGGGGTGGGA CTCTCTGTGG 
ATTGGCTATT CCTTCATGAT GCATACAAGT GCAGGAGCAG AAGGCTCTGG CCAAGCCCTA 
GCCTCTCCTG GCTCCTGTTT GGAAGAATTT CGTTCCGCTC CCTTCATCGA ATGTCATGGG 
CGAGGGACAT GCAATTACTA TGCCAATTCG TACAGCTTTT GGCTGGCCAC TGTTGATATG 
TCAGACATGT TCAACAAACC TCAGTCAGAA ACCCTGAAAG CAGGAGACTT GAGGACGCGT 
ATTAGCCGAT GTCAAGTATG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a5 ( NM_001163155.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156627.1 Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant 1, mRNA.

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CloneID	OMu00702
Clone ID Related Accession (Same CDS sequence)	NM_007736.4 , NM_001163155.1
Accession Version	NM_001163155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	collagen alpha-5(IV) chain precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL713983.9, CN668672.1, AK133179.1 and BX571779.6. Transcript Variant: This variant (1) represents the longer transcript. Both variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC082788.1, AB041350.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAAGTGC GTGGAGTGTG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCCG CAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGGTTTCCAG GTTTAGAAGG TCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGCCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGGATGCCA GGCCATGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC GGGCTTTCCT 
GGTTTACAAG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGGGA ACCAGGCAGT 
ATAATCATGT CACCACTGCC AGGACCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGCC CACCTGGTCC CAAGGGTATA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GACCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGC GAACAAGGTC TTCAGGGCCC ACCTGGTCCA 
CCTGGGCAGA TCAGCGAACA GAAAAGACCA ATTGACATAG AATTTCAGAA AGGAGATCAG 
GGAGTTCCTG GTGATAGAGG GCCTCCTGGA CCTCCAGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCAGGAGAGG CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG CCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGAGAAC CAGGAAGAGA TGGTGAAAAG GGTCAAAAGG GTGATATTGG CCCACCTGGA 
CCTCCCGGAC TTGTAATTCC TGCACCTGGA ACTGGTGTGA CTATGGGAGA AAAAGGAAAT 
ATCGGGTTAC CTGGTTTGCC TGGAGAAAAG GGAGAGCGAG GATTTCCTGG AATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGAACA GCAGTTGTGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGCCTTC CAGGACCTCC TGGCCCCCCT 
GGCCCCCAGT TGCCATCCAG AGATGAAATC TGTAAAGCAG GCCCTCCTGG GCCTCCAGGA 
CCTCCAGGTG ATAAAGGACT CCAAGGAGAG CGAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCTGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGGGACAA AGGACAAGCT 
GGGATAACTG GTCCGAAAGG ATTGCCAGGC ATACCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGGGATGTC CTCACTCTTC CAGGAATGAA AGGTGACAAA 
GGAGAGTTGG GTTTCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTCCC TGGCCCCAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CTCCTGGGAG CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCAGTTG GTTTTGGGCC TCCAGGCCCC ATAGGCGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCGGG ATTACCAGGT CCTAAAGGAG ATCCAGGCCA GACCATAACC 
CAGCCAGGAA AGCCTGGTTT GCCAGGTAGT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCTGGA CCACCTGGTC CTAAAGGTTT TCCAGGAATT 
CCGGGACCTC CAGGAGCTCC TGGGGCCCCT GGAAGAATGG GTCCAGATGG GCCTCCTGGG 
CCACCAGGCT TCCCAGGACC AAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTTCAG GTCCTCCAGG ACGCACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGCCCAGTA GGGCCTCCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGG 
ATTCCAGGAG AGAAGGGGGA CCCAGGACCT CCTGGGTTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTGTCCCTG GAAAAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC TGGACCTTTG GGAATTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGATGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGC AGGAGAAAAG 
GGCGGTAAGG GAGAGCCTGG CCTTCCAGGT CCTCCTGGAC CAATGCATCC AGATTTCCTG 
GGATCGAAAG GAGAAAAGGG TGAGCCCGGC TTACCAGGTA TTCCTGGACT TTCAGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCAGGA 
TCACCAGGAC TCCCAGGTCC CAAGGGTAAT CCTGGTCTCC CTGGTCCACC AGGACTTACA 
GGACCTCCTG GACCAAAAGG AAACATTGGT GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCCC CTGGACCTCC TGGATTTCCT GGACAACCTG GCTCTCCTGG GTTACCTGGC 
CAGAAAGGAA GTAAAGGAGA GCCTGGGGTT TCAGGCATTG GACTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGGTCGGTG 
GGAGAGACTG GTCTTCCTGG ATTACCAGGA AACCCTGGAG CAAAAGGTCA ACCGGGCCTG 
CCTGGATTCC CAGGAACACC AGGACTTCCT GGACCAAAAG GTATGAATGG TCCTCCTGGT 
AACCCTGGCT TTCCCGGAGA ACCTGGTCCT GTAGGTGCCG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGGCC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGACAAA AGGGTGATGG AGGATCACCT GGACTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AGCCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCTCCT 
GGTTCTCCTG GTCAAGCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCTCCAGG TTTTCAAGGT CCACCAGGTA CAGAAGGTCC TCGTGGACCC 
CCTGGAAATG GAGGTATCAA AGGAGAGAGG GGAAATCCAG GCCCACCTGG GCAGCCAGGC 
TTACCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC CGGGTAACCC TGGCCGGCCA 
GGTCTTAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGAATG 
AAAGGACCCA TTGGAGTACC AGGCTCCACT GGCCCTGATG GGGAACCAGG TCTTACTGGG 
CCCCCAGGTC CTCCTGGGTT GCCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT 
CCTGGACCTC CAGGGATTCC TGGACAACCT GGATTAAAAG GTCTACCAGG ACTTCCAGGC 
CCTCAAGGCC TACCAGGTCC AATTGGCCCT CCAGGAGATC CAGGACGCAA TGGACTCCCA 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGGC TGCCAGGACA GCCAGGTGCC 
CGTGGATTGG ATGGTCCCCC AGGACCAGAT GGATTGCAAG GACCTCCAGG ACCCCCTGGA 
ACCACCTCTG TTGCCCATGG ATTCCTCATC ACACGTCACA GTCAAACAAC AGAAGCACCA 
CAATGCCCAC GGGGAACAGT TCATATCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CCCATGGTCA AGACTTGGGT ACGGCTGGCA GCTGCCTTCG TCGCTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATTAAC AACGTTTGTA ACTTTGCTTC AAGAAATGAC 
TATTCTTACT GGCTTTCCAC CCCAGAGCCC ATGCCAATGA ACATGGAACC CCTGAAGGGA 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTGTGTG AGGCGCCAGC TGTGGTGATC 
GCTGTTCACA GCCAGACTAT TCAGATTCCA CACTGTCCTC AGGGGTGGGA CTCTCTGTGG 
ATTGGCTATT CCTTCATGAT GCATACAAGT GCAGGAGCAG AAGGCTCTGG CCAAGCCCTA 
GCCTCTCCTG GCTCCTGTTT GGAAGAATTT CGTTCCGCTC CCTTCATCGA ATGTCATGGG 
CGAGGGACAT GCAATTACTA TGCCAATTCG TACAGCTTTT GGCTGGCCAC TGTTGATATG 
TCAGACATGT TCAACAAACC TCAGTCAGAA ACCCTGAAAG CAGGAGACTT GAGGACGCGT 
ATTAGCCGAT GTCAAGTATG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156627.1
CDS	317..5392
Translation	MQVRGVCLAAGWFLLALCLWGQPAEAAACHGCSSGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSPLPGPKGNQGYPGPPGIQGPPGPKGISGPSGPPGPPGLMGPPGPPGPPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDIEFQKGDQGVPGDRGPPGPPGIPGPPGPPGGVKGEKGEPGEAGKRGKPGKDGETGQPGIPGLPGDPGYPGEPGRDGEKGQKGDIGPPGPPGLVIPAPGTGVTMGEKGNIGLPGLPGEKGERGFPGIQGPPGFPGPPGTAVVGPPGPPGYPGERGQKGDEGPPGICIPGSPGLDGQPGAPGLPGPPGPPGPQLPSRDEICKAGPPGPPGPPGDKGLQGERGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGIPGEKGDKGQAGITGPKGLPGIPGPPGAPGFPGSKGDPGDVLTLPGMKGDKGELGFPGAPGLPGLPGSPGKDGLPGLPGPKGEPGGITFKGERGPPGSPGLPGLPGNMGPTGPVGFGPPGPIGEKGIQGVAGNPGQPGLPGPKGDPGQTITQPGKPGLPGSPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGPPGPPGPKGFPGIPGPPGAPGAPGRMGPDGPPGPPGFPGPKGEPGFGLPGPPGPPGFPGFKGTPGPKGDRGFPGPSGPPGRTGLDGLPGPKGDVGPNGQPGPVGPPGLPGIGLQGPPGPPGTPGPIGQPGLHGIPGEKGDPGPPGFDVPGLPGERGSPGLPGAPGLIGPPGSPGVPGKAGVPGFPGVKGEMGMMGPPGPPGPLGIPGRSGAPGLKGDDGMQGQPGLPGPAGEKGGKGEPGLPGPPGPMHPDFLGSKGEKGEPGLPGIPGLSGPKGYQGLPGDPGQPGLSGQPGSPGLPGPKGNPGLPGPPGLTGPPGPKGNIGDMGFPGPQGVDGPPGPPGFPGQPGSPGLPGQKGSKGEPGVSGIGLPGLPGPKGEPGLPGYPGNPGIKGSVGETGLPGLPGNPGAKGQPGLPGFPGTPGLPGPKGMNGPPGNPGFPGEPGPVGAGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDGGSPGLPGNPGLPGSKGEPGFQGFPGMPGPPGPPGSPGQALEGPKGNPGPQGPPGRPGPPGFQGPPGTEGPRGPPGNGGIKGERGNPGPPGQPGLPGLKGDQGPPGLPGNPGRPGLNGMKGDPGLPGVPGFPGMKGPIGVPGSTGPDGEPGLTGPPGPPGLPGPSGQSIVIKGDPGPPGIPGQPGLKGLPGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGARGLDGPPGPDGLQGPPGPPGTTSVAHGFLITRHSQTTEAPQCPRGTVHIYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMNMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDMSDMFNKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	NM_001163155.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163155.1

ORF Insert Sequence:

ATGCAAGTGC GTGGAGTGTG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCCG CAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGGTTTCCAG GTTTAGAAGG TCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGCCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGGATGCCA GGCCATGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC GGGCTTTCCT 
GGTTTACAAG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGGGA ACCAGGCAGT 
ATAATCATGT CACCACTGCC AGGACCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGCC CACCTGGTCC CAAGGGTATA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GACCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGC GAACAAGGTC TTCAGGGCCC ACCTGGTCCA 
CCTGGGCAGA TCAGCGAACA GAAAAGACCA ATTGACATAG AATTTCAGAA AGGAGATCAG 
GGAGTTCCTG GTGATAGAGG GCCTCCTGGA CCTCCAGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCAGGAGAGG CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG CCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGAGAAC CAGGAAGAGA TGGTGAAAAG GGTCAAAAGG GTGATATTGG CCCACCTGGA 
CCTCCCGGAC TTGTAATTCC TGCACCTGGA ACTGGTGTGA CTATGGGAGA AAAAGGAAAT 
ATCGGGTTAC CTGGTTTGCC TGGAGAAAAG GGAGAGCGAG GATTTCCTGG AATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGAACA GCAGTTGTGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGCCTTC CAGGACCTCC TGGCCCCCCT 
GGCCCCCAGT TGCCATCCAG AGATGAAATC TGTAAAGCAG GCCCTCCTGG GCCTCCAGGA 
CCTCCAGGTG ATAAAGGACT CCAAGGAGAG CGAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCTGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGGGACAA AGGACAAGCT 
GGGATAACTG GTCCGAAAGG ATTGCCAGGC ATACCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGGGATGTC CTCACTCTTC CAGGAATGAA AGGTGACAAA 
GGAGAGTTGG GTTTCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTCCC TGGCCCCAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CTCCTGGGAG CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCAGTTG GTTTTGGGCC TCCAGGCCCC ATAGGCGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCGGG ATTACCAGGT CCTAAAGGAG ATCCAGGCCA GACCATAACC 
CAGCCAGGAA AGCCTGGTTT GCCAGGTAGT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCTGGA CCACCTGGTC CTAAAGGTTT TCCAGGAATT 
CCGGGACCTC CAGGAGCTCC TGGGGCCCCT GGAAGAATGG GTCCAGATGG GCCTCCTGGG 
CCACCAGGCT TCCCAGGACC AAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTTCAG GTCCTCCAGG ACGCACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGCCCAGTA GGGCCTCCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGG 
ATTCCAGGAG AGAAGGGGGA CCCAGGACCT CCTGGGTTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTGTCCCTG GAAAAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC TGGACCTTTG GGAATTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGATGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGC AGGAGAAAAG 
GGCGGTAAGG GAGAGCCTGG CCTTCCAGGT CCTCCTGGAC CAATGCATCC AGATTTCCTG 
GGATCGAAAG GAGAAAAGGG TGAGCCCGGC TTACCAGGTA TTCCTGGACT TTCAGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCAGGA 
TCACCAGGAC TCCCAGGTCC CAAGGGTAAT CCTGGTCTCC CTGGTCCACC AGGACTTACA 
GGACCTCCTG GACCAAAAGG AAACATTGGT GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCCC CTGGACCTCC TGGATTTCCT GGACAACCTG GCTCTCCTGG GTTACCTGGC 
CAGAAAGGAA GTAAAGGAGA GCCTGGGGTT TCAGGCATTG GACTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGGTCGGTG 
GGAGAGACTG GTCTTCCTGG ATTACCAGGA AACCCTGGAG CAAAAGGTCA ACCGGGCCTG 
CCTGGATTCC CAGGAACACC AGGACTTCCT GGACCAAAAG GTATGAATGG TCCTCCTGGT 
AACCCTGGCT TTCCCGGAGA ACCTGGTCCT GTAGGTGCCG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGGCC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGACAAA AGGGTGATGG AGGATCACCT GGACTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AGCCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCTCCT 
GGTTCTCCTG GTCAAGCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCTCCAGG TTTTCAAGGT CCACCAGGTA CAGAAGGTCC TCGTGGACCC 
CCTGGAAATG GAGGTATCAA AGGAGAGAGG GGAAATCCAG GCCCACCTGG GCAGCCAGGC 
TTACCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC CGGGTAACCC TGGCCGGCCA 
GGTCTTAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGAATG 
AAAGGACCCA TTGGAGTACC AGGCTCCACT GGCCCTGATG GGGAACCAGG TCTTACTGGG 
CCCCCAGGTC CTCCTGGGTT GCCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT 
CCTGGACCTC CAGGGATTCC TGGACAACCT GGATTAAAAG GTCTACCAGG ACTTCCAGGC 
CCTCAAGGCC TACCAGGTCC AATTGGCCCT CCAGGAGATC CAGGACGCAA TGGACTCCCA 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGGC TGCCAGGACA GCCAGGTGCC 
CGTGGATTGG ATGGTCCCCC AGGACCAGAT GGATTGCAAG GACCTCCAGG ACCCCCTGGA 
ACCACCTCTG TTGCCCATGG ATTCCTCATC ACACGTCACA GTCAAACAAC AGAAGCACCA 
CAATGCCCAC GGGGAACAGT TCATATCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CCCATGGTCA AGACTTGGGT ACGGCTGGCA GCTGCCTTCG TCGCTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATTAAC AACGTTTGTA ACTTTGCTTC AAGAAATGAC 
TATTCTTACT GGCTTTCCAC CCCAGAGCCC ATGCCAATGA ACATGGAACC CCTGAAGGGA 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTGTGTG AGGCGCCAGC TGTGGTGATC 
GCTGTTCACA GCCAGACTAT TCAGATTCCA CACTGTCCTC AGGGGTGGGA CTCTCTGTGG 
ATTGGCTATT CCTTCATGAT GCATACAAGT GCAGGAGCAG AAGGCTCTGG CCAAGCCCTA 
GCCTCTCCTG GCTCCTGTTT GGAAGAATTT CGTTCCGCTC CCTTCATCGA ATGTCATGGG 
CGAGGGACAT GCAATTACTA TGCCAATTCG TACAGCTTTT GGCTGGCCAC TGTTGATATG 
TCAGACATGT TCAACAAACC TCAGTCAGAA ACCCTGAAAG CAGGAGACTT GAGGACGCGT 
ATTAGCCGAT GTCAAGTATG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a5 ( XM_006528696.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528759.1 Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant X1, mRNA.

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CloneID	OMu56583
Clone ID Related Accession (Same CDS sequence)	XM_006528696.3
Accession Version	XM_006528696.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-5(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528696.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTGC GTGGAGTGTG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCCG CAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGGTTTCCAG GTTTAGAAGG TCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGCCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGGATGCCA GGCCATGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC GGGCTTTCCT 
GGTTTACAAG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGGGA ACCAGGCAGT 
ATAATCATGT CACCACTGCC AGGACCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGCC CACCTGGTCC CAAGGGTATA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GACCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGC GAACAAGGTC TTCAGGGCCC ACCTGGTCCA 
CCTGGGCAGA TCAGCGAACA GAAAAGACCA ATTGACATAG AATTTCAGAA AGGAGATCAG 
GGAGTTCCTG GTGATAGAGG GCCTCCTGGA CCTCCAGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCAGGAGAGG CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG CCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGAGAAC CAGGAAGAGA TGGTGAAAAG GGTCAAAAGG GTGATATTGG CCCACCTGGA 
CCTCCCGGAC TTGTAATTCC TGCACCTGGA ACTGGTGTGA CTATGGGAGA AAAAGGAAAT 
ATCGGGTTAC CTGGTTTGCC TGGAGAAAAG GGAGAGCGAG GATTTCCTGG AATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGAACA GCAGTTGTGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGCCTTC CAGGACCTCC TGGCCCCCCT 
GGCCCCCAGT TGCCATCCAG AGATGAAATC TGTAAAGCAG GCCCTCCTGG GCCTCCAGGA 
CCTCCAGGTG ATAAAGGACT CCAAGGAGAG CGAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCTGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGGGACAA AGGACAAGCT 
GGGATAACTG GTCCGAAAGG ATTGCCAGGC ATACCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGGGATGTC CTCACTCTTC CAGGAATGAA AGGTGACAAA 
GGAGAGTTGG GTTTCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTCCC TGGCCCCAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CTCCTGGGAG CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCAGTTG GTTTTGGGCC TCCAGGCCCC ATAGGCGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCGGG ATTACCAGGT CCTAAAGGAG ATCCAGGCCA GACCATAACC 
CAGCCAGGAA AGCCTGGTTT GCCAGGTAGT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCTGGA CCACCTGGTC CTAAAGGTTT TCCAGGAATT 
CCGGGACCTC CAGGAGCTCC TGGGGCCCCT GGAAGAATGG GTCCAGATGG GCCTCCTGGG 
CCACCAGGCT TCCCAGGACC AAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTTCAG GTCCTCCAGG ACGCACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGCCCAGTA GGGCCTCCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGG 
ATTCCAGGAG AGAAGGGGGA CCCAGGACCT CCTGGGTTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTGTCCCTG GAAAAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC TGGACCTTTG GGAATTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGATGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGC AGGAGAAAAG 
GGCGGTAAGG GAGAGCCTGG CCTTCCAGGT CCTCCTGGAC CAATGCATCC AGATTTCCTG 
GGATCGAAAG GAGAAAAGGG TGAGCCCGGC TTACCAGGTA TTCCTGGACT TTCAGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCAGGA 
TCACCAGGAC TCCCAGGTCC CAAGGGTAAT CCTGGTCTCC CTGGTCCACC AGGACTTACA 
GGACCTCCTG GACCAAAAGG AAACATTGGT GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCCC CTGGACCTCC TGGATTTCCT GGACAACCTG GCTCTCCTGG GTTACCTGGC 
CAGAAAGGAA GTAAAGGAGA GCCTGGGGTT TCAGGCATTG GACTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGGTCGGTG 
GGAGAGACTG GTCTTCCTGG ATTACCAGGA AACCCTGGAG CAAAAGGTCA ACCGGGCCTG 
CCTGGATTCC CAGGAACACC AGGACTTCCT GGACCAAAAG GTATGAATGG TCCTCCTGGT 
AACCCTGGCT TTCCCGGAGA ACCTGGTCCT GTAGGTGCCG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGGCC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGACAAA AGGGTGATGG AGGATCACCT GGACTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AGCCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCTCCT 
GGTTCTCCTG GTCAAGCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTTTTCAAGG TCCACCAGGT ACAGAAGGTC CTCGTGGACC CCCTGGAAAT 
GGAGGTATCA AAGGAGAGAG GGGAAATCCA GGCCCACCTG GGCAGCCAGG CTTACCTGGT 
TTGAAAGGAG ATCAAGGACC ACCAGGACTC CCGGGTAACC CTGGCCGGCC AGGTCTTAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC 
ATTGGAGTAC CAGGCTCCAC TGGCCCTGAT GGGGAACCAG GTCTTACTGG GCCCCCAGGT 
CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT 
CCAGGGATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGC 
CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT 
GGTGCAGGAG GGCGCAAAGG AGACCCAGGG CTGCCAGGAC AGCCAGGTGC CCGTGGATTG 
GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GACCCCCTGG AACCACCTCT 
GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAAACAA CAGAAGCACC ACAATGCCCA 
CGGGGAACAG TTCATATCTA TGAAGGCTTT TCTCTCCTGT ATGTACAAGG AAATAAAAGA 
GCCCATGGTC AAGACTTGGG TACGGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT 
TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC 
TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG ACAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTGTGT GAGGCGCCAG CTGTGGTGAT CGCTGTTCAC 
AGCCAGACTA TTCAGATTCC ACACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT 
TCCTTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT 
GGCTCCTGTT TGGAAGAATT TCGTTCCGCT CCCTTCATCG AATGTCATGG GCGAGGGACA 
TGCAATTACT ATGCCAATTC GTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG 
TTCAACAAAC CTCAGTCAGA AACCCTGAAA GCAGGAGACT TGAGGACGCG TATTAGCCGA 
TGTCAAGTAT GCATGAAGAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528759.1
CDS	366..5432
Translation	MQVRGVCLAAGWFLLALCLWGQPAEAAACHGCSSGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSPLPGPKGNQGYPGPPGIQGPPGPKGISGPSGPPGPPGLMGPPGPPGPPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDIEFQKGDQGVPGDRGPPGPPGIPGPPGPPGGVKGEKGEPGEAGKRGKPGKDGETGQPGIPGLPGDPGYPGEPGRDGEKGQKGDIGPPGPPGLVIPAPGTGVTMGEKGNIGLPGLPGEKGERGFPGIQGPPGFPGPPGTAVVGPPGPPGYPGERGQKGDEGPPGICIPGSPGLDGQPGAPGLPGPPGPPGPQLPSRDEICKAGPPGPPGPPGDKGLQGERGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGIPGEKGDKGQAGITGPKGLPGIPGPPGAPGFPGSKGDPGDVLTLPGMKGDKGELGFPGAPGLPGLPGSPGKDGLPGLPGPKGEPGGITFKGERGPPGSPGLPGLPGNMGPTGPVGFGPPGPIGEKGIQGVAGNPGQPGLPGPKGDPGQTITQPGKPGLPGSPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGPPGPPGPKGFPGIPGPPGAPGAPGRMGPDGPPGPPGFPGPKGEPGFGLPGPPGPPGFPGFKGTPGPKGDRGFPGPSGPPGRTGLDGLPGPKGDVGPNGQPGPVGPPGLPGIGLQGPPGPPGTPGPIGQPGLHGIPGEKGDPGPPGFDVPGLPGERGSPGLPGAPGLIGPPGSPGVPGKAGVPGFPGVKGEMGMMGPPGPPGPLGIPGRSGAPGLKGDDGMQGQPGLPGPAGEKGGKGEPGLPGPPGPMHPDFLGSKGEKGEPGLPGIPGLSGPKGYQGLPGDPGQPGLSGQPGSPGLPGPKGNPGLPGPPGLTGPPGPKGNIGDMGFPGPQGVDGPPGPPGFPGQPGSPGLPGQKGSKGEPGVSGIGLPGLPGPKGEPGLPGYPGNPGIKGSVGETGLPGLPGNPGAKGQPGLPGFPGTPGLPGPKGMNGPPGNPGFPGEPGPVGAGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDGGSPGLPGNPGLPGSKGEPGFQGFPGMPGPPGPPGSPGQALEGPKGNPGPQGPPGRPGFQGPPGTEGPRGPPGNGGIKGERGNPGPPGQPGLPGLKGDQGPPGLPGNPGRPGLNGMKGDPGLPGVPGFPGMKGPIGVPGSTGPDGEPGLTGPPGPPGLPGPSGQSIVIKGDPGPPGIPGQPGLKGLPGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGARGLDGPPGPDGLQGPPGPPGTTSVAHGFLITRHSQTTEAPQCPRGTVHIYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMNMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDMSDMFNKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006528696.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528696.3

ORF Insert Sequence:

ATGCAAGTGC GTGGAGTGTG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCCG CAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGGTTTCCAG GTTTAGAAGG TCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGCCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGGATGCCA GGCCATGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC GGGCTTTCCT 
GGTTTACAAG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGGGA ACCAGGCAGT 
ATAATCATGT CACCACTGCC AGGACCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGCC CACCTGGTCC CAAGGGTATA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GACCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGC GAACAAGGTC TTCAGGGCCC ACCTGGTCCA 
CCTGGGCAGA TCAGCGAACA GAAAAGACCA ATTGACATAG AATTTCAGAA AGGAGATCAG 
GGAGTTCCTG GTGATAGAGG GCCTCCTGGA CCTCCAGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCAGGAGAGG CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG CCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGAGAAC CAGGAAGAGA TGGTGAAAAG GGTCAAAAGG GTGATATTGG CCCACCTGGA 
CCTCCCGGAC TTGTAATTCC TGCACCTGGA ACTGGTGTGA CTATGGGAGA AAAAGGAAAT 
ATCGGGTTAC CTGGTTTGCC TGGAGAAAAG GGAGAGCGAG GATTTCCTGG AATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGAACA GCAGTTGTGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGCCTTC CAGGACCTCC TGGCCCCCCT 
GGCCCCCAGT TGCCATCCAG AGATGAAATC TGTAAAGCAG GCCCTCCTGG GCCTCCAGGA 
CCTCCAGGTG ATAAAGGACT CCAAGGAGAG CGAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCTGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGGGACAA AGGACAAGCT 
GGGATAACTG GTCCGAAAGG ATTGCCAGGC ATACCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGGGATGTC CTCACTCTTC CAGGAATGAA AGGTGACAAA 
GGAGAGTTGG GTTTCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTCCC TGGCCCCAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CTCCTGGGAG CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCAGTTG GTTTTGGGCC TCCAGGCCCC ATAGGCGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCGGG ATTACCAGGT CCTAAAGGAG ATCCAGGCCA GACCATAACC 
CAGCCAGGAA AGCCTGGTTT GCCAGGTAGT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCTGGA CCACCTGGTC CTAAAGGTTT TCCAGGAATT 
CCGGGACCTC CAGGAGCTCC TGGGGCCCCT GGAAGAATGG GTCCAGATGG GCCTCCTGGG 
CCACCAGGCT TCCCAGGACC AAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTTCAG GTCCTCCAGG ACGCACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGCCCAGTA GGGCCTCCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGG 
ATTCCAGGAG AGAAGGGGGA CCCAGGACCT CCTGGGTTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTGTCCCTG GAAAAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC TGGACCTTTG GGAATTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGATGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGC AGGAGAAAAG 
GGCGGTAAGG GAGAGCCTGG CCTTCCAGGT CCTCCTGGAC CAATGCATCC AGATTTCCTG 
GGATCGAAAG GAGAAAAGGG TGAGCCCGGC TTACCAGGTA TTCCTGGACT TTCAGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCAGGA 
TCACCAGGAC TCCCAGGTCC CAAGGGTAAT CCTGGTCTCC CTGGTCCACC AGGACTTACA 
GGACCTCCTG GACCAAAAGG AAACATTGGT GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCCC CTGGACCTCC TGGATTTCCT GGACAACCTG GCTCTCCTGG GTTACCTGGC 
CAGAAAGGAA GTAAAGGAGA GCCTGGGGTT TCAGGCATTG GACTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGGTCGGTG 
GGAGAGACTG GTCTTCCTGG ATTACCAGGA AACCCTGGAG CAAAAGGTCA ACCGGGCCTG 
CCTGGATTCC CAGGAACACC AGGACTTCCT GGACCAAAAG GTATGAATGG TCCTCCTGGT 
AACCCTGGCT TTCCCGGAGA ACCTGGTCCT GTAGGTGCCG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGGCC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGACAAA AGGGTGATGG AGGATCACCT GGACTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AGCCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCTCCT 
GGTTCTCCTG GTCAAGCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTTTTCAAGG TCCACCAGGT ACAGAAGGTC CTCGTGGACC CCCTGGAAAT 
GGAGGTATCA AAGGAGAGAG GGGAAATCCA GGCCCACCTG GGCAGCCAGG CTTACCTGGT 
TTGAAAGGAG ATCAAGGACC ACCAGGACTC CCGGGTAACC CTGGCCGGCC AGGTCTTAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC 
ATTGGAGTAC CAGGCTCCAC TGGCCCTGAT GGGGAACCAG GTCTTACTGG GCCCCCAGGT 
CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT 
CCAGGGATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGC 
CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT 
GGTGCAGGAG GGCGCAAAGG AGACCCAGGG CTGCCAGGAC AGCCAGGTGC CCGTGGATTG 
GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GACCCCCTGG AACCACCTCT 
GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAAACAA CAGAAGCACC ACAATGCCCA 
CGGGGAACAG TTCATATCTA TGAAGGCTTT TCTCTCCTGT ATGTACAAGG AAATAAAAGA 
GCCCATGGTC AAGACTTGGG TACGGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT 
TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC 
TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG ACAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTGTGT GAGGCGCCAG CTGTGGTGAT CGCTGTTCAC 
AGCCAGACTA TTCAGATTCC ACACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT 
TCCTTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT 
GGCTCCTGTT TGGAAGAATT TCGTTCCGCT CCCTTCATCG AATGTCATGG GCGAGGGACA 
TGCAATTACT ATGCCAATTC GTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG 
TTCAACAAAC CTCAGTCAGA AACCCTGAAA GCAGGAGACT TGAGGACGCG TATTAGCCGA 
TGTCAAGTAT GCATGAAGAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a5 ( XM_006528697.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528760.1 Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant X2, mRNA.

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CloneID	OMu56584
Clone ID Related Accession (Same CDS sequence)	XM_006528697.3 , XM_006528697.4
Accession Version	XM_006528697.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-5(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528697.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTGC GTGGAGTGTG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCCG CAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGGTTTCCAG GTTTAGAAGG TCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGCCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGGATGCCA GGCCATGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC GGGCTTTCCT 
GGTTTACAAG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGGGA ACCAGGCAGT 
ATAATCATGT CACCACTGCC AGGACCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGCC CACCTGGTCC CAAGGGTATA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GACCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGC GAACAAGGTC TTCAGGGCCC ACCTGGTCCA 
CCTGGGCAGA TCAGCGAACA GAAAAGACCA ATTGACATAG AATTTCAGAA AGGAGATCAG 
GGAGTTCCTG GTGATAGAGG GCCTCCTGGA CCTCCAGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCAGGAGAGG CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG CCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGAGAAC CAGGAAGAGA TGGTGAAAAG GGTCAAAAGG GTGATATTGG CCCACCTGGA 
CCTCCCGGAC TTGTAATTCC TGCACCTGGA ACTGGTGTGA CTATGGGAGA AAAAGGAAAT 
ATCGGGTTAC CTGGTTTGCC TGGAGAAAAG GGAGAGCGAG GATTTCCTGG AATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGAACA GCAGTTGTGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGCCTTC CAGGACCTCC TGGCCCCCCT 
GGCCCCCAGT TGCCATCCAG AGATGAAATC TGTAAAGCAG GCCCTCCTGG GCCTCCAGGA 
CCTCCAGGTG ATAAAGGACT CCAAGGAGAG CGAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCTGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGGGACAA AGGACAAGCT 
GGGATAACTG GTCCGAAAGG ATTGCCAGGC ATACCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGGGATGTC CTCACTCTTC CAGGAATGAA AGGTGACAAA 
GGAGAGTTGG GTTTCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTCCC TGGCCCCAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CTCCTGGGAG CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCAGTTG GTTTTGGGCC TCCAGGCCCC ATAGGCGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCGGG ATTACCAGGT CCTAAAGGAG ATCCAGGCCA GACCATAACC 
CAGCCAGGAA AGCCTGGTTT GCCAGGTAGT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCTGGA CCACCTGGTC CTAAAGGTTT TCCAGGAATT 
CCGGGACCTC CAGGAGCTCC TGGGGCCCCT GGAAGAATGG GTCCAGATGG GCCTCCTGGG 
CCACCAGGCT TCCCAGGACC AAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTTCAG GTCCTCCAGG ACGCACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGCCCAGTA GGGCCTCCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGG 
ATTCCAGGAG AGAAGGGGGA CCCAGGACCT CCTGGGTTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTGTCCCTG GAAAAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC TGGACCTTTG GGAATTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGATGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGC AGGAGAAAAG 
GGCGGTAAGG GAGAGCCTGG CCTTCCAGGT CCTCCTGGAC CAATGCATCC AGATTTCCTG 
GGATCGAAAG GAGAAAAGGG TGAGCCCGGC TTACCAGGTA TTCCTGGACT TTCAGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCAGGA 
TCACCAGGAC TCCCAGGTCC CAAGGGTAAT CCTGGTCTCC CTGGTCCACC AGGACTTACA 
GGACCTCCTG GACCAAAAGG AAACATTGGT GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCCC CTGGACCTCC TGGATTTCCT GGACAACCTG GCTCTCCTGG GTTACCTGGC 
CAGAAAGGAA GTAAAGGAGA GCCTGGGGTT TCAGGCATTG GACTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGGTCGGTG 
GGAGAGACTG GTCTTCCTGG ATTACCAGGA AACCCTGGAG CAAAAGGTCA ACCGGGCCTG 
CCTGGATTCC CAGGAACACC AGGACTTCCT GGACCAAAAG GTATGAATGG TCCTCCTGGT 
AACCCTGGCT TTCCCGGAGA ACCTGGTCCT GTAGGTGCCG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGGCC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGACAAA AGGGTGATGG AGGATCACCT GGACTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AGCCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCTCCT 
GGTTCTCCTG GTCAAGCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCACCAGG TACAGAAGGT CCTCGTGGAC CCCCTGGAAA TGGAGGTATC 
AAAGGAGAGA GGGGAAATCC AGGCCCACCT GGGCAGCCAG GCTTACCTGG TTTGAAAGGA 
GATCAAGGAC CACCAGGACT CCCGGGTAAC CCTGGCCGGC CAGGTCTTAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGAA TGAAAGGACC CATTGGAGTA 
CCAGGCTCCA CTGGCCCTGA TGGGGAACCA GGTCTTACTG GGCCCCCAGG TCCTCCTGGG 
TTGCCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATCCTGGACC TCCAGGGATT 
CCTGGACAAC CTGGATTAAA AGGTCTACCA GGACTTCCAG GCCCTCAAGG CCTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCAGGACGC AATGGACTCC CAGGCTTTGA TGGTGCAGGA 
GGGCGCAAAG GAGACCCAGG GCTGCCAGGA CAGCCAGGTG CCCGTGGATT GGATGGTCCC 
CCAGGACCAG ATGGATTGCA AGGACCTCCA GGACCCCCTG GAACCACCTC TGTTGCCCAT 
GGATTCCTCA TCACACGTCA CAGTCAAACA ACAGAAGCAC CACAATGCCC ACGGGGAACA 
GTTCATATCT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCCCATGGT 
CAAGACTTGG GTACGGCTGG CAGCTGCCTT CGTCGCTTCA GTACCATGCC TTTCATGTTC 
TGCAACATTA ACAACGTTTG TAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTTTCC 
ACCCCAGAGC CCATGCCAAT GAACATGGAA CCCCTGAAGG GACAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTGTG TGAGGCGCCA GCTGTGGTGA TCGCTGTTCA CAGCCAGACT 
ATTCAGATTC CACACTGTCC TCAGGGGTGG GACTCTCTGT GGATTGGCTA TTCCTTCATG 
ATGCATACAA GTGCAGGAGC AGAAGGCTCT GGCCAAGCCC TAGCCTCTCC TGGCTCCTGT 
TTGGAAGAAT TTCGTTCCGC TCCCTTCATC GAATGTCATG GGCGAGGGAC ATGCAATTAC 
TATGCCAATT CGTACAGCTT TTGGCTGGCC ACTGTTGATA TGTCAGACAT GTTCAACAAA 
CCTCAGTCAG AAACCCTGAA AGCAGGAGAC TTGAGGACGC GTATTAGCCG ATGTCAAGTA 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528760.1
CDS	366..5423
Translation	MQVRGVCLAAGWFLLALCLWGQPAEAAACHGCSSGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSPLPGPKGNQGYPGPPGIQGPPGPKGISGPSGPPGPPGLMGPPGPPGPPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDIEFQKGDQGVPGDRGPPGPPGIPGPPGPPGGVKGEKGEPGEAGKRGKPGKDGETGQPGIPGLPGDPGYPGEPGRDGEKGQKGDIGPPGPPGLVIPAPGTGVTMGEKGNIGLPGLPGEKGERGFPGIQGPPGFPGPPGTAVVGPPGPPGYPGERGQKGDEGPPGICIPGSPGLDGQPGAPGLPGPPGPPGPQLPSRDEICKAGPPGPPGPPGDKGLQGERGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGIPGEKGDKGQAGITGPKGLPGIPGPPGAPGFPGSKGDPGDVLTLPGMKGDKGELGFPGAPGLPGLPGSPGKDGLPGLPGPKGEPGGITFKGERGPPGSPGLPGLPGNMGPTGPVGFGPPGPIGEKGIQGVAGNPGQPGLPGPKGDPGQTITQPGKPGLPGSPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGPPGPPGPKGFPGIPGPPGAPGAPGRMGPDGPPGPPGFPGPKGEPGFGLPGPPGPPGFPGFKGTPGPKGDRGFPGPSGPPGRTGLDGLPGPKGDVGPNGQPGPVGPPGLPGIGLQGPPGPPGTPGPIGQPGLHGIPGEKGDPGPPGFDVPGLPGERGSPGLPGAPGLIGPPGSPGVPGKAGVPGFPGVKGEMGMMGPPGPPGPLGIPGRSGAPGLKGDDGMQGQPGLPGPAGEKGGKGEPGLPGPPGPMHPDFLGSKGEKGEPGLPGIPGLSGPKGYQGLPGDPGQPGLSGQPGSPGLPGPKGNPGLPGPPGLTGPPGPKGNIGDMGFPGPQGVDGPPGPPGFPGQPGSPGLPGQKGSKGEPGVSGIGLPGLPGPKGEPGLPGYPGNPGIKGSVGETGLPGLPGNPGAKGQPGLPGFPGTPGLPGPKGMNGPPGNPGFPGEPGPVGAGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDGGSPGLPGNPGLPGSKGEPGFQGFPGMPGPPGPPGSPGQALEGPKGNPGPQGPPGRPGPPGTEGPRGPPGNGGIKGERGNPGPPGQPGLPGLKGDQGPPGLPGNPGRPGLNGMKGDPGLPGVPGFPGMKGPIGVPGSTGPDGEPGLTGPPGPPGLPGPSGQSIVIKGDPGPPGIPGQPGLKGLPGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGARGLDGPPGPDGLQGPPGPPGTTSVAHGFLITRHSQTTEAPQCPRGTVHIYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMNMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDMSDMFNKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006528697.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528697.3

ORF Insert Sequence:

ATGCAAGTGC GTGGAGTGTG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCCG CAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGGTTTCCAG GTTTAGAAGG TCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGCCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGGATGCCA GGCCATGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC GGGCTTTCCT 
GGTTTACAAG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGGGA ACCAGGCAGT 
ATAATCATGT CACCACTGCC AGGACCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGCC CACCTGGTCC CAAGGGTATA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GACCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAAGGC GAACAAGGTC TTCAGGGCCC ACCTGGTCCA 
CCTGGGCAGA TCAGCGAACA GAAAAGACCA ATTGACATAG AATTTCAGAA AGGAGATCAG 
GGAGTTCCTG GTGATAGAGG GCCTCCTGGA CCTCCAGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCAGGAGAGG CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG CCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGAGAAC CAGGAAGAGA TGGTGAAAAG GGTCAAAAGG GTGATATTGG CCCACCTGGA 
CCTCCCGGAC TTGTAATTCC TGCACCTGGA ACTGGTGTGA CTATGGGAGA AAAAGGAAAT 
ATCGGGTTAC CTGGTTTGCC TGGAGAAAAG GGAGAGCGAG GATTTCCTGG AATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGAACA GCAGTTGTGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGCCTTC CAGGACCTCC TGGCCCCCCT 
GGCCCCCAGT TGCCATCCAG AGATGAAATC TGTAAAGCAG GCCCTCCTGG GCCTCCAGGA 
CCTCCAGGTG ATAAAGGACT CCAAGGAGAG CGAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCTGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGGGACAA AGGACAAGCT 
GGGATAACTG GTCCGAAAGG ATTGCCAGGC ATACCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGGGATGTC CTCACTCTTC CAGGAATGAA AGGTGACAAA 
GGAGAGTTGG GTTTCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTCCC TGGCCCCAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CTCCTGGGAG CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCAGTTG GTTTTGGGCC TCCAGGCCCC ATAGGCGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCGGG ATTACCAGGT CCTAAAGGAG ATCCAGGCCA GACCATAACC 
CAGCCAGGAA AGCCTGGTTT GCCAGGTAGT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCTGGA CCACCTGGTC CTAAAGGTTT TCCAGGAATT 
CCGGGACCTC CAGGAGCTCC TGGGGCCCCT GGAAGAATGG GTCCAGATGG GCCTCCTGGG 
CCACCAGGCT TCCCAGGACC AAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTTCAG GTCCTCCAGG ACGCACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGCCCAGTA GGGCCTCCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGG 
ATTCCAGGAG AGAAGGGGGA CCCAGGACCT CCTGGGTTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTGTCCCTG GAAAAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC TGGACCTTTG GGAATTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGATGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGC AGGAGAAAAG 
GGCGGTAAGG GAGAGCCTGG CCTTCCAGGT CCTCCTGGAC CAATGCATCC AGATTTCCTG 
GGATCGAAAG GAGAAAAGGG TGAGCCCGGC TTACCAGGTA TTCCTGGACT TTCAGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCAGGA 
TCACCAGGAC TCCCAGGTCC CAAGGGTAAT CCTGGTCTCC CTGGTCCACC AGGACTTACA 
GGACCTCCTG GACCAAAAGG AAACATTGGT GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCCC CTGGACCTCC TGGATTTCCT GGACAACCTG GCTCTCCTGG GTTACCTGGC 
CAGAAAGGAA GTAAAGGAGA GCCTGGGGTT TCAGGCATTG GACTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGGTCGGTG 
GGAGAGACTG GTCTTCCTGG ATTACCAGGA AACCCTGGAG CAAAAGGTCA ACCGGGCCTG 
CCTGGATTCC CAGGAACACC AGGACTTCCT GGACCAAAAG GTATGAATGG TCCTCCTGGT 
AACCCTGGCT TTCCCGGAGA ACCTGGTCCT GTAGGTGCCG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGGCC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGACAAA AGGGTGATGG AGGATCACCT GGACTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AGCCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCTCCT 
GGTTCTCCTG GTCAAGCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCACCAGG TACAGAAGGT CCTCGTGGAC CCCCTGGAAA TGGAGGTATC 
AAAGGAGAGA GGGGAAATCC AGGCCCACCT GGGCAGCCAG GCTTACCTGG TTTGAAAGGA 
GATCAAGGAC CACCAGGACT CCCGGGTAAC CCTGGCCGGC CAGGTCTTAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGAA TGAAAGGACC CATTGGAGTA 
CCAGGCTCCA CTGGCCCTGA TGGGGAACCA GGTCTTACTG GGCCCCCAGG TCCTCCTGGG 
TTGCCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATCCTGGACC TCCAGGGATT 
CCTGGACAAC CTGGATTAAA AGGTCTACCA GGACTTCCAG GCCCTCAAGG CCTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCAGGACGC AATGGACTCC CAGGCTTTGA TGGTGCAGGA 
GGGCGCAAAG GAGACCCAGG GCTGCCAGGA CAGCCAGGTG CCCGTGGATT GGATGGTCCC 
CCAGGACCAG ATGGATTGCA AGGACCTCCA GGACCCCCTG GAACCACCTC TGTTGCCCAT 
GGATTCCTCA TCACACGTCA CAGTCAAACA ACAGAAGCAC CACAATGCCC ACGGGGAACA 
GTTCATATCT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCCCATGGT 
CAAGACTTGG GTACGGCTGG CAGCTGCCTT CGTCGCTTCA GTACCATGCC TTTCATGTTC 
TGCAACATTA ACAACGTTTG TAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTTTCC 
ACCCCAGAGC CCATGCCAAT GAACATGGAA CCCCTGAAGG GACAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTGTG TGAGGCGCCA GCTGTGGTGA TCGCTGTTCA CAGCCAGACT 
ATTCAGATTC CACACTGTCC TCAGGGGTGG GACTCTCTGT GGATTGGCTA TTCCTTCATG 
ATGCATACAA GTGCAGGAGC AGAAGGCTCT GGCCAAGCCC TAGCCTCTCC TGGCTCCTGT 
TTGGAAGAAT TTCGTTCCGC TCCCTTCATC GAATGTCATG GGCGAGGGAC ATGCAATTAC 
TATGCCAATT CGTACAGCTT TTGGCTGGCC ACTGTTGATA TGTCAGACAT GTTCAACAAA 
CCTCAGTCAG AAACCCTGAA AGCAGGAGAC TTGAGGACGC GTATTAGCCG ATGTCAAGTA 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a5 ( XM_017318372.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173861.1 Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant X3, mRNA.

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CloneID	OMu83066
Clone ID Related Accession (Same CDS sequence)	XM_017318372.2 , XM_017318372.1
Accession Version	XM_017318372.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-5(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTGC GTGGAGTGTG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCCG CAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGGTTTCCAG GTTTAGAAGG TCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGCCCT CCAGGACCTC GGGGACAAAA GGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGGA TGCCAGGCCA TGATGGGGCT 
CCAGGACCTC AAGGTATCCC TGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCGGGCT TTCCTGGTTT ACAAGGTCCT CCAGGACCTC CTGGGATCCC AGGTATGAAG 
GGGGAACCAG GCAGTATAAT CATGTCACCA CTGCCAGGAC CAAAGGGTAA TCAAGGATAC 
CCAGGTCCTC CTGGAATACA AGGCCCACCT GGTCCCAAGG GTATATCAGG GCCAAGTGGT 
CCTCCAGGAC CACCAGGTTT AATGGGACCT CCTGGCCCAC CAGGCCCACC GGGACCAAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGCGAACA AGGTCTTCAG 
GGCCCACCTG GTCCACCTGG GCAGATCAGC GAACAGAAAA GACCAATTGA CATAGAATTT 
CAGAAAGGAG ATCAGGGAGT TCCTGGTGAT AGAGGGCCTC CTGGACCTCC AGGAATACCT 
GGTCCTCCCG GTCCTCCTGG TGGTGTGAAA GGTGAAAAGG GTGAGCCAGG AGAGGCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAG ACTGGCCAAC CAGGAATCCC AGGTTTGCCT 
GGTGATCCTG GTTACCCTGG AGAACCAGGA AGAGATGGTG AAAAGGGTCA AAAGGGTGAT 
ATTGGCCCAC CTGGACCTCC CGGACTTGTA ATTCCTGCAC CTGGAACTGG TGTGACTATG 
GGAGAAAAAG GAAATATCGG GTTACCTGGT TTGCCTGGAG AAAAGGGAGA GCGAGGATTT 
CCTGGAATAC AAGGTCCACC TGGCTTTCCT GGACCTCCAG GAACAGCAGT TGTGGGTCCC 
CCTGGTCCTC CTGGATATCC TGGTGAAAGG GGCCAGAAAG GTGATGAAGG TCCCCCTGGA 
ATTTGTATTC CTGGATCTCC TGGACTTGAT GGACAGCCTG GGGCTCCTGG CCTTCCAGGA 
CCTCCTGGCC CCCCTGGCCC CCAGTTGCCA TCCAGAGATG AAATCTGTAA AGCAGGCCCT 
CCTGGGCCTC CAGGACCTCC AGGTGATAAA GGACTCCAAG GAGAGCGAGG AGTAAAAGGT 
GACAAAGGTG ATACTTGCTT CAACTGTATT GGAACTGGCA TTTCAGGGCC TCCAGGCCAA 
CCTGGTTTAC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GAATCCCTGG AGAGAAGGGG 
GACAAAGGAC AAGCTGGGAT AACTGGTCCG AAAGGATTGC CAGGCATACC TGGACCTCCA 
GGTGCTCCAG GCTTTCCAGG GTCTAAGGGT GACCCTGGGG ATGTCCTCAC TCTTCCAGGA 
ATGAAAGGTG ACAAAGGAGA GTTGGGTTTC CCTGGAGCTC CAGGGCTTCC TGGTCTACCT 
GGTAGTCCTG GAAAGGATGG TTTGCCAGGA CTCCCTGGCC CCAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCTCCT GGGAGCCCAG GTTTACCAGG TCTCCCAGGA 
AATATGGGAC CTACAGGCCC AGTTGGTTTT GGGCCTCCAG GCCCCATAGG CGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCGGGATTAC CAGGTCCTAA AGGAGATCCA 
GGCCAGACCA TAACCCAGCC AGGAAAGCCT GGTTTGCCAG GTAGTCCAGG CAGAGATGGT 
GAAGTGGGTC TTCCAGGTGA TCCTGGACTT CCAGGCCAAC CAGGCCTGCC AGGAATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGG ATTGGGCCTC CTGGACCACC TGGTCCTAAA 
GGTTTTCCAG GAATTCCGGG ACCTCCAGGA GCTCCTGGGG CCCCTGGAAG AATGGGTCCA 
GATGGGCCTC CTGGGCCACC AGGCTTCCCA GGACCAAAGG GAGAGCCAGG ATTTGGGTTA 
CCTGGGCCAC CTGGGCCACC AGGATTCCCA GGTTTCAAAG GAACACCTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TTCAGGTCCT CCAGGACGCA CTGGCTTGGA TGGGCTACCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGCC CAGTAGGGCC TCCAGGGCTG 
CCAGGAATTG GTCTTCAGGG ACCACCAGGA CCACCAGGGA CTCCAGGGCC AATAGGCCAA 
CCTGGCTTAC ATGGGATTCC AGGAGAGAAG GGGGACCCAG GACCTCCTGG GTTTGATGTT 
CCAGGACTCC CTGGAGAGAG AGGTAGTCCA GGGCTCCCTG GAGCCCCTGG TCTTATAGGA 
CCTCCAGGAT CACCTGGTGT CCCTGGAAAA GCAGGTGTCC CTGGATTTCC AGGTGTCAAA 
GGCGAAATGG GTATGATGGG ACCTCCAGGC CCACCTGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGCTC CTGGTCTTAA AGGTGATGAT GGCATGCAAG GTCAGCCAGG TCTCCCTGGC 
CCTGCAGGAG AAAAGGGCGG TAAGGGAGAG CCTGGCCTTC CAGGTCCTCC TGGACCAATG 
CATCCAGATT TCCTGGGATC GAAAGGAGAA AAGGGTGAGC CCGGCTTACC AGGTATTCCT 
GGACTTTCAG GACCAAAAGG CTACCAGGGA TTACCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CAGGATCACC AGGACTCCCA GGTCCCAAGG GTAATCCTGG TCTCCCTGGT 
CCACCAGGAC TTACAGGACC TCCTGGACCA AAAGGAAACA TTGGTGACAT GGGTTTTCCT 
GGCCCTCAAG GTGTAGATGG GCCCCCTGGA CCTCCTGGAT TTCCTGGACA ACCTGGCTCT 
CCTGGGTTAC CTGGCCAGAA AGGAAGTAAA GGAGAGCCTG GGGTTTCAGG CATTGGACTT 
CCAGGTCTTC CTGGCCCAAA GGGTGAACCT GGTCTGCCTG GATATCCAGG AAACCCAGGT 
ATCAAAGGGT CGGTGGGAGA GACTGGTCTT CCTGGATTAC CAGGAAACCC TGGAGCAAAA 
GGTCAACCGG GCCTGCCTGG ATTCCCAGGA ACACCAGGAC TTCCTGGACC AAAAGGTATG 
AATGGTCCTC CTGGTAACCC TGGCTTTCCC GGAGAACCTG GTCCTGTAGG TGCCGGAGGG 
CGTCCTGGAC CACCAGGACC TCCTGGTGAA AAAGGCAAGC CAGGTCAAGA TGGCATTCCT 
GGGCCAGCAG GACAAAAGGG AGAACCAGGT CAACCAGGCT TTGGAATCCC AGGACCTCCT 
GGACTCCCAG GACTTTCTGG ACAAAAGGGT GATGGAGGAT CACCTGGACT TCCAGGAAAT 
CCTGGCCTTC CAGGTTCAAA GGGTGAGCCA GGCTTTCAGG GATTCCCTGG TATGCCAGGT 
CCCCCAGGTC CTCCTGGTTC TCCTGGTCAA GCTTTGGAAG GTCCCAAAGG AAACCCTGGG 
CCTCAAGGCC CTCCTGGGAG ACCAGGTCCT CCAGGTTTTC AAGGTCCACC AGGTACAGAA 
GGTCCTCGTG GACCCCCTGG AAATGGAGGT ATCAAAGGAG AGAGGGGAAA TCCAGGCCCA 
CCTGGGCAGC CAGGCTTACC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCCGGGT 
AACCCTGGCC GGCCAGGTCT TAATGGAATG AAAGGAGATC CTGGTCTCCC TGGTGTTCCA 
GGATTCCCAG GAATGAAAGG ACCCATTGGA GTACCAGGCT CCACTGGCCC TGATGGGGAA 
CCAGGTCTTA CTGGGCCCCC AGGTCCTCCT GGGTTGCCTG GTCCTTCAGG ACAGAGTATT 
GTAATCAAAG GAGATCCTGG ACCTCCAGGG ATTCCTGGAC AACCTGGATT AAAAGGTCTA 
CCAGGACTTC CAGGCCCTCA AGGCCTACCA GGTCCAATTG GCCCTCCAGG AGATCCAGGA 
CGCAATGGAC TCCCAGGCTT TGATGGTGCA GGAGGGCGCA AAGGAGACCC AGGGCTGCCA 
GGACAGCCAG GTGCCCGTGG ATTGGATGGT CCCCCAGGAC CAGATGGATT GCAAGGACCT 
CCAGGACCCC CTGGAACCAC CTCTGTTGCC CATGGATTCC TCATCACACG TCACAGTCAA 
ACAACAGAAG CACCACAATG CCCACGGGGA ACAGTTCATA TCTATGAAGG CTTTTCTCTC 
CTGTATGTAC AAGGAAATAA AAGAGCCCAT GGTCAAGACT TGGGTACGGC TGGCAGCTGC 
CTTCGTCGCT TCAGTACCAT GCCTTTCATG TTCTGCAACA TTAACAACGT TTGTAACTTT 
GCTTCAAGAA ATGACTATTC TTACTGGCTT TCCACCCCAG AGCCCATGCC AATGAACATG 
GAACCCCTGA AGGGACAGAG CATCCAGCCA TTCATTAGTC GATGTGCAGT GTGTGAGGCG 
CCAGCTGTGG TGATCGCTGT TCACAGCCAG ACTATTCAGA TTCCACACTG TCCTCAGGGG 
TGGGACTCTC TGTGGATTGG CTATTCCTTC ATGATGCATA CAAGTGCAGG AGCAGAAGGC 
TCTGGCCAAG CCCTAGCCTC TCCTGGCTCC TGTTTGGAAG AATTTCGTTC CGCTCCCTTC 
ATCGAATGTC ATGGGCGAGG GACATGCAAT TACTATGCCA ATTCGTACAG CTTTTGGCTG 
GCCACTGTTG ATATGTCAGA CATGTTCAAC AAACCTCAGT CAGAAACCCT GAAAGCAGGA 
GACTTGAGGA CGCGTATTAG CCGATGTCAA GTATGCATGA AGAGGACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173861.1
CDS	366..5396
Translation	MQVRGVCLAAGWFLLALCLWGQPAEAAACHGCSSGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSPLPGPKGNQGYPGPPGIQGPPGPKGISGPSGPPGPPGLMGPPGPPGPPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDIEFQKGDQGVPGDRGPPGPPGIPGPPGPPGGVKGEKGEPGEAGKRGKPGKDGETGQPGIPGLPGDPGYPGEPGRDGEKGQKGDIGPPGPPGLVIPAPGTGVTMGEKGNIGLPGLPGEKGERGFPGIQGPPGFPGPPGTAVVGPPGPPGYPGERGQKGDEGPPGICIPGSPGLDGQPGAPGLPGPPGPPGPQLPSRDEICKAGPPGPPGPPGDKGLQGERGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGIPGEKGDKGQAGITGPKGLPGIPGPPGAPGFPGSKGDPGDVLTLPGMKGDKGELGFPGAPGLPGLPGSPGKDGLPGLPGPKGEPGGITFKGERGPPGSPGLPGLPGNMGPTGPVGFGPPGPIGEKGIQGVAGNPGQPGLPGPKGDPGQTITQPGKPGLPGSPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGPPGPPGPKGFPGIPGPPGAPGAPGRMGPDGPPGPPGFPGPKGEPGFGLPGPPGPPGFPGFKGTPGPKGDRGFPGPSGPPGRTGLDGLPGPKGDVGPNGQPGPVGPPGLPGIGLQGPPGPPGTPGPIGQPGLHGIPGEKGDPGPPGFDVPGLPGERGSPGLPGAPGLIGPPGSPGVPGKAGVPGFPGVKGEMGMMGPPGPPGPLGIPGRSGAPGLKGDDGMQGQPGLPGPAGEKGGKGEPGLPGPPGPMHPDFLGSKGEKGEPGLPGIPGLSGPKGYQGLPGDPGQPGLSGQPGSPGLPGPKGNPGLPGPPGLTGPPGPKGNIGDMGFPGPQGVDGPPGPPGFPGQPGSPGLPGQKGSKGEPGVSGIGLPGLPGPKGEPGLPGYPGNPGIKGSVGETGLPGLPGNPGAKGQPGLPGFPGTPGLPGPKGMNGPPGNPGFPGEPGPVGAGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDGGSPGLPGNPGLPGSKGEPGFQGFPGMPGPPGPPGSPGQALEGPKGNPGPQGPPGRPGPPGFQGPPGTEGPRGPPGNGGIKGERGNPGPPGQPGLPGLKGDQGPPGLPGNPGRPGLNGMKGDPGLPGVPGFPGMKGPIGVPGSTGPDGEPGLTGPPGPPGLPGPSGQSIVIKGDPGPPGIPGQPGLKGLPGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGARGLDGPPGPDGLQGPPGPPGTTSVAHGFLITRHSQTTEAPQCPRGTVHIYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMNMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDMSDMFNKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_017318372.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IV, alpha 5 (Col4a5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318372.1

ORF Insert Sequence:

ATGCAAGTGC GTGGAGTGTG CCTGGCTGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCCG CAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGGTTTCCAG GTTTAGAAGG TCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGCCCT CCAGGACCTC GGGGACAAAA GGGTCCTCCT 
GGACTTCCTG GATTTCCAGG GACACCAGGT CTTCCTGGGA TGCCAGGCCA TGATGGGGCT 
CCAGGACCTC AAGGTATCCC TGGATGCAAT GGAACCAAGG GAGAACGTGG ATTTCCAGGC 
AGTCCGGGCT TTCCTGGTTT ACAAGGTCCT CCAGGACCTC CTGGGATCCC AGGTATGAAG 
GGGGAACCAG GCAGTATAAT CATGTCACCA CTGCCAGGAC CAAAGGGTAA TCAAGGATAC 
CCAGGTCCTC CTGGAATACA AGGCCCACCT GGTCCCAAGG GTATATCAGG GCCAAGTGGT 
CCTCCAGGAC CACCAGGTTT AATGGGACCT CCTGGCCCAC CAGGCCCACC GGGACCAAAG 
GGGAATATGG GCTTAAATTT CCAGGGACCC AAAGGTGAAA AAGGCGAACA AGGTCTTCAG 
GGCCCACCTG GTCCACCTGG GCAGATCAGC GAACAGAAAA GACCAATTGA CATAGAATTT 
CAGAAAGGAG ATCAGGGAGT TCCTGGTGAT AGAGGGCCTC CTGGACCTCC AGGAATACCT 
GGTCCTCCCG GTCCTCCTGG TGGTGTGAAA GGTGAAAAGG GTGAGCCAGG AGAGGCAGGC 
AAAAGAGGTA AACCAGGCAA AGATGGAGAG ACTGGCCAAC CAGGAATCCC AGGTTTGCCT 
GGTGATCCTG GTTACCCTGG AGAACCAGGA AGAGATGGTG AAAAGGGTCA AAAGGGTGAT 
ATTGGCCCAC CTGGACCTCC CGGACTTGTA ATTCCTGCAC CTGGAACTGG TGTGACTATG 
GGAGAAAAAG GAAATATCGG GTTACCTGGT TTGCCTGGAG AAAAGGGAGA GCGAGGATTT 
CCTGGAATAC AAGGTCCACC TGGCTTTCCT GGACCTCCAG GAACAGCAGT TGTGGGTCCC 
CCTGGTCCTC CTGGATATCC TGGTGAAAGG GGCCAGAAAG GTGATGAAGG TCCCCCTGGA 
ATTTGTATTC CTGGATCTCC TGGACTTGAT GGACAGCCTG GGGCTCCTGG CCTTCCAGGA 
CCTCCTGGCC CCCCTGGCCC CCAGTTGCCA TCCAGAGATG AAATCTGTAA AGCAGGCCCT 
CCTGGGCCTC CAGGACCTCC AGGTGATAAA GGACTCCAAG GAGAGCGAGG AGTAAAAGGT 
GACAAAGGTG ATACTTGCTT CAACTGTATT GGAACTGGCA TTTCAGGGCC TCCAGGCCAA 
CCTGGTTTAC CAGGTCTCCC AGGTCCTCCA GGATCTCTTG GAATCCCTGG AGAGAAGGGG 
GACAAAGGAC AAGCTGGGAT AACTGGTCCG AAAGGATTGC CAGGCATACC TGGACCTCCA 
GGTGCTCCAG GCTTTCCAGG GTCTAAGGGT GACCCTGGGG ATGTCCTCAC TCTTCCAGGA 
ATGAAAGGTG ACAAAGGAGA GTTGGGTTTC CCTGGAGCTC CAGGGCTTCC TGGTCTACCT 
GGTAGTCCTG GAAAGGATGG TTTGCCAGGA CTCCCTGGCC CCAAAGGAGA GCCTGGTGGA 
ATTACTTTTA AGGGTGAAAG AGGTCCTCCT GGGAGCCCAG GTTTACCAGG TCTCCCAGGA 
AATATGGGAC CTACAGGCCC AGTTGGTTTT GGGCCTCCAG GCCCCATAGG CGAAAAAGGC 
ATACAAGGTG TGGCAGGAAA TCCAGGCCAG CCGGGATTAC CAGGTCCTAA AGGAGATCCA 
GGCCAGACCA TAACCCAGCC AGGAAAGCCT GGTTTGCCAG GTAGTCCAGG CAGAGATGGT 
GAAGTGGGTC TTCCAGGTGA TCCTGGACTT CCAGGCCAAC CAGGCCTGCC AGGAATACCT 
GGTAGCAAAG GAGAACCAGG TATCCCTGGG ATTGGGCCTC CTGGACCACC TGGTCCTAAA 
GGTTTTCCAG GAATTCCGGG ACCTCCAGGA GCTCCTGGGG CCCCTGGAAG AATGGGTCCA 
GATGGGCCTC CTGGGCCACC AGGCTTCCCA GGACCAAAGG GAGAGCCAGG ATTTGGGTTA 
CCTGGGCCAC CTGGGCCACC AGGATTCCCA GGTTTCAAAG GAACACCTGG TCCAAAAGGT 
GATCGTGGTT TCCCAGGACC TTCAGGTCCT CCAGGACGCA CTGGCTTGGA TGGGCTACCT 
GGACCAAAAG GTGATGTTGG ACCAAATGGA CAACCTGGCC CAGTAGGGCC TCCAGGGCTG 
CCAGGAATTG GTCTTCAGGG ACCACCAGGA CCACCAGGGA CTCCAGGGCC AATAGGCCAA 
CCTGGCTTAC ATGGGATTCC AGGAGAGAAG GGGGACCCAG GACCTCCTGG GTTTGATGTT 
CCAGGACTCC CTGGAGAGAG AGGTAGTCCA GGGCTCCCTG GAGCCCCTGG TCTTATAGGA 
CCTCCAGGAT CACCTGGTGT CCCTGGAAAA GCAGGTGTCC CTGGATTTCC AGGTGTCAAA 
GGCGAAATGG GTATGATGGG ACCTCCAGGC CCACCTGGAC CTTTGGGAAT TCCTGGCAGG 
AGTGGTGCTC CTGGTCTTAA AGGTGATGAT GGCATGCAAG GTCAGCCAGG TCTCCCTGGC 
CCTGCAGGAG AAAAGGGCGG TAAGGGAGAG CCTGGCCTTC CAGGTCCTCC TGGACCAATG 
CATCCAGATT TCCTGGGATC GAAAGGAGAA AAGGGTGAGC CCGGCTTACC AGGTATTCCT 
GGACTTTCAG GACCAAAAGG CTACCAGGGA TTACCTGGAG ACCCAGGGCA ACCTGGACTG 
AGTGGACAAC CAGGATCACC AGGACTCCCA GGTCCCAAGG GTAATCCTGG TCTCCCTGGT 
CCACCAGGAC TTACAGGACC TCCTGGACCA AAAGGAAACA TTGGTGACAT GGGTTTTCCT 
GGCCCTCAAG GTGTAGATGG GCCCCCTGGA CCTCCTGGAT TTCCTGGACA ACCTGGCTCT 
CCTGGGTTAC CTGGCCAGAA AGGAAGTAAA GGAGAGCCTG GGGTTTCAGG CATTGGACTT 
CCAGGTCTTC CTGGCCCAAA GGGTGAACCT GGTCTGCCTG GATATCCAGG AAACCCAGGT 
ATCAAAGGGT CGGTGGGAGA GACTGGTCTT CCTGGATTAC CAGGAAACCC TGGAGCAAAA 
GGTCAACCGG GCCTGCCTGG ATTCCCAGGA ACACCAGGAC TTCCTGGACC AAAAGGTATG 
AATGGTCCTC CTGGTAACCC TGGCTTTCCC GGAGAACCTG GTCCTGTAGG TGCCGGAGGG 
CGTCCTGGAC CACCAGGACC TCCTGGTGAA AAAGGCAAGC CAGGTCAAGA TGGCATTCCT 
GGGCCAGCAG GACAAAAGGG AGAACCAGGT CAACCAGGCT TTGGAATCCC AGGACCTCCT 
GGACTCCCAG GACTTTCTGG ACAAAAGGGT GATGGAGGAT CACCTGGACT TCCAGGAAAT 
CCTGGCCTTC CAGGTTCAAA GGGTGAGCCA GGCTTTCAGG GATTCCCTGG TATGCCAGGT 
CCCCCAGGTC CTCCTGGTTC TCCTGGTCAA GCTTTGGAAG GTCCCAAAGG AAACCCTGGG 
CCTCAAGGCC CTCCTGGGAG ACCAGGTCCT CCAGGTTTTC AAGGTCCACC AGGTACAGAA 
GGTCCTCGTG GACCCCCTGG AAATGGAGGT ATCAAAGGAG AGAGGGGAAA TCCAGGCCCA 
CCTGGGCAGC CAGGCTTACC TGGTTTGAAA GGAGATCAAG GACCACCAGG ACTCCCGGGT 
AACCCTGGCC GGCCAGGTCT TAATGGAATG AAAGGAGATC CTGGTCTCCC TGGTGTTCCA 
GGATTCCCAG GAATGAAAGG ACCCATTGGA GTACCAGGCT CCACTGGCCC TGATGGGGAA 
CCAGGTCTTA CTGGGCCCCC AGGTCCTCCT GGGTTGCCTG GTCCTTCAGG ACAGAGTATT 
GTAATCAAAG GAGATCCTGG ACCTCCAGGG ATTCCTGGAC AACCTGGATT AAAAGGTCTA 
CCAGGACTTC CAGGCCCTCA AGGCCTACCA GGTCCAATTG GCCCTCCAGG AGATCCAGGA 
CGCAATGGAC TCCCAGGCTT TGATGGTGCA GGAGGGCGCA AAGGAGACCC AGGGCTGCCA 
GGACAGCCAG GTGCCCGTGG ATTGGATGGT CCCCCAGGAC CAGATGGATT GCAAGGACCT 
CCAGGACCCC CTGGAACCAC CTCTGTTGCC CATGGATTCC TCATCACACG TCACAGTCAA 
ACAACAGAAG CACCACAATG CCCACGGGGA ACAGTTCATA TCTATGAAGG CTTTTCTCTC 
CTGTATGTAC AAGGAAATAA AAGAGCCCAT GGTCAAGACT TGGGTACGGC TGGCAGCTGC 
CTTCGTCGCT TCAGTACCAT GCCTTTCATG TTCTGCAACA TTAACAACGT TTGTAACTTT 
GCTTCAAGAA ATGACTATTC TTACTGGCTT TCCACCCCAG AGCCCATGCC AATGAACATG 
GAACCCCTGA AGGGACAGAG CATCCAGCCA TTCATTAGTC GATGTGCAGT GTGTGAGGCG 
CCAGCTGTGG TGATCGCTGT TCACAGCCAG ACTATTCAGA TTCCACACTG TCCTCAGGGG 
TGGGACTCTC TGTGGATTGG CTATTCCTTC ATGATGCATA CAAGTGCAGG AGCAGAAGGC 
TCTGGCCAAG CCCTAGCCTC TCCTGGCTCC TGTTTGGAAG AATTTCGTTC CGCTCCCTTC 
ATCGAATGTC ATGGGCGAGG GACATGCAAT TACTATGCCA ATTCGTACAG CTTTTGGCTG 
GCCACTGTTG ATATGTCAGA CATGTTCAAC AAACCTCAGT CAGAAACCCT GAAAGCAGGA 
GACTTGAGGA CGCGTATTAG CCGATGTCAA GTATGCATGA AGAGGACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pathogenicity of a Human Laminin
Journal of the American Society of Nephrology : JASN(2017 Dec)
Funk SD,Bayer RH,Malone AF,McKee KK,Yurchenco PD,Miner JH

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

A step towards clinical application of acellular matrix: A clue from macrophage polarization.
Matrix biology : journal of the International Society for Matrix Biology57-58334-346(2017 Jan)
Petrosyan A,Da Sacco S,Tripuraneni N,Kreuser U,Lavarreda-Pearce M,Tamburrini R,De Filippo RE,Orlando G,Cravedi P,Perin L

A potential target gene for the host-directed therapy of mycobacterial infection in murine macrophages.
International journal of molecular medicine38(3)823-33(2016 Sep)
Bao Z,Chen R,Zhang P,Lu S,Chen X,Yao Y,Jin X,Sun Y,Zhou J

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Col4a5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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