SPEG cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPEG
Entrez Gene ID	10290
Full Name	SPEG complex locus
Synonyms	APEG-1,APEG1,BPEG,CNM5,SPEGalpha,SPEGbeta
General protein information	Preferred Names SPEG complex locus Names striated muscle preferentially expressed protein kinase aortic preferentially expressed gene 1 aortic preferentially expressed protein 1 nuclear protein, marker for differentiated aortic smooth muscle and down-regulated with vascular injury
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q35
Summary	This gene encodes a protein with similarity to members of the myosin light chain kinase family. This protein family is required for myocyte cytoskeletal development. Along with the desmin gene, expression of this gene may be controlled by the desmin locus control region. Mutations in this gene are associated with centronuclear myopathy 5. [provided by RefSeq, Jun 2016].
Disorder MIM:	615950
Disorder Html:	Centronuclear myopathy 5, 615959 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017003159.1	XP_016858648.1	striated muscle preferentially expressed protein kinase isoform X8
XM_017003161.1	XP_016858650.1	striated muscle preferentially expressed protein kinase isoform X11
NM_005876.5	NP_005867.3	striated muscle preferentially expressed protein kinase isoform 1
XM_005246237.2	XP_005246294.1	striated muscle preferentially expressed protein kinase isoform X5
XM_024452526.1	XP_024308294.1	striated muscle preferentially expressed protein kinase isoform X13
XM_017003158.1	XP_016858647.1	striated muscle preferentially expressed protein kinase isoform X4
NM_001173476.1	NP_001166947.1	striated muscle preferentially expressed protein kinase isoform 4
XM_011510483.2	XP_011508785.2	striated muscle preferentially expressed protein kinase isoform X1
XM_011510479.2	XP_011508781.1	striated muscle preferentially expressed protein kinase isoform X3
XM_006712189.3	XP_006712252.1	striated muscle preferentially expressed protein kinase isoform X6
XM_005246242.4	XP_005246299.1	striated muscle preferentially expressed protein kinase isoform X9
XM_005246241.1	XP_005246298.1	striated muscle preferentially expressed protein kinase isoform X4
XM_017003160.1	XP_016858649.1	striated muscle preferentially expressed protein kinase isoform X10
XM_017003162.1	XP_016858651.1	striated muscle preferentially expressed protein kinase isoform X12
XM_017003157.1	XP_016858646.1	striated muscle preferentially expressed protein kinase isoform X2
XM_006712193.3	XP_006712256.1	striated muscle preferentially expressed protein kinase isoform X4
XM_017003161.2	XP_016858650.1	striated muscle preferentially expressed protein kinase isoform X11
XM_017003162.2	XP_016858651.1	striated muscle preferentially expressed protein kinase isoform X12
NM_005876.4	NP_005867.3	striated muscle preferentially expressed protein kinase isoform 1
XM_005246239.2	XP_005246296.1	striated muscle preferentially expressed protein kinase isoform X7
NM_001173476.2	NP_001166947.1	striated muscle preferentially expressed protein kinase isoform 4
XM_017003158.2	XP_016858647.1	striated muscle preferentially expressed protein kinase isoform X4
XM_017003159.2	XP_016858648.1	striated muscle preferentially expressed protein kinase isoform X8
XM_005246240.2	XP_005246297.1	striated muscle preferentially expressed protein kinase isoform X4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SPEG	NP_001135957.1
Mus musculus (house mouse)	Speg	NP_031489.4
Rattus norvegicus (Norway rat)	Speg	NP_001102272.1
Gallus gallus (chicken)	SPEG	XP_003641690.2
Danio rerio (zebrafish)	si:ch211-195m20.1	XP_691634.6
Homo sapiens (human)	SPEG	NP_005867.3
Pan troglodytes (chimpanzee)	SPEG	NP_001166952.1
	SPEG	XP_001104264.2
Canis lupus familiaris (dog)	SPEG	NP_001166949.1
Xenopus tropicalis (tropical clawed frog)	speg	XP_002934009.2

Gene Ontology
Bibliography

Related articles in PubMed

SPEG (Striated Muscle Preferentially Expressed Protein Kinase) Is Essential for Cardiac Function by Regulating Junctional Membrane Complex Activity.
Quick AP, Wang Q, Philippen LE, Barreto-Torres G, Chiang DY, Beavers D, Wang G, Khalid M, Reynolds JO, Campbell HM, Showell J, McCauley MD, Scholten A, Wehrens XH
Circulation research120(1)110-119(2017 Jan)

SPEG interacts with myotubularin, and its deficiency causes centronuclear myopathy with dilated cardiomyopathy.
Agrawal PB, Pierson CR, Joshi M, Liu X, Ravenscroft G, Moghadaszadeh B, Talabere T, Viola M, Swanson LC, Halilo?lu G, Talim B, Yau KS, Allcock RJ, Laing NG, Perrella MA, Beggs AH
American journal of human genetics95(2)218-26(2014 Aug)

The human desmin locus: gene organization and LCR-mediated transcriptional control.
Tam JL, Triantaphyllopoulos K, Todd H, Raguz S, de Wit T, Morgan JE, Partridge TA, Makrinou E, Grosveld F, Antoniou M
Genomics87(6)733-46(2006 Jun)

X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1).
Manjasetty BA, Niesen FH, Scheich C, Roske Y, Goetz F, Behlke J, Sievert V, Heinemann U, B?ssow K
BMC structural biology521(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPEG is present in cardiac muscle, where it plays a critical role; therefore, individuals with SPEG mutations additionally present with dilated cardiomyopathy.
Title: SPEG interacts with myotubularin, and its deficiency causes centronuclear myopathy with dilated cardiomyopathy.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

the RGD motif might play a role not only in the adhesion of Aortic Preferentially Expressed Protein-1 and extracellular proteins but also in intracellular protein-protein interactions
Title: X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1).

Genomic rearrangement on APEG1 in arteriosclerosis was studied.
Title: Genomic rearrangements on VCAM1, SELE, APEG1and AIF1 loci in atherosclerosis.

The following SPEG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPEG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01307	NM_001173476.2 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu01307	XM_024452526.1 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu88922	XM_017003161.2 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu88923	XM_017003162.2 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu24274	NM_005876.5 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu35751	XM_005246237.2 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu35752	XM_006712189.3 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu35755	XM_005246239.2 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu35757	XM_005246242.4 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu35758	XM_006712193.3 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu35758	XM_005246240.2 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu35758	XM_005246241.1 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu35758	XM_017003158.2 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu63876	XM_011510479.2 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu88918	XM_011510483.2 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu88919	XM_017003157.1 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu88920	XM_017003159.2 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu88921	XM_017003160.1 Latest version!	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
View Old Accession Versions >>
OHu88922	XM_017003161.1	Homo sapiens SPEG complex locus (SPEG), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu88923	XM_017003162.1	Homo sapiens SPEG complex locus (SPEG), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu24274	NM_005876.4	Homo sapiens SPEG complex locus (SPEG), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu01307	NM_001173476.1	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu35758	XM_017003158.1	Homo sapiens SPEG complex locus (SPEG), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu88920	XM_017003159.1	Homo sapiens SPEG complex locus (SPEG), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPEG ( NM_001173476.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166947.1 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu01307
Clone ID Related Accession (Same CDS sequence)	XM_024452526.1 , NM_001173476.1 , NM_001173476.2
Accession Version	NM_001173476.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-20
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA063220.1, AC053503.7 and BM677138.1. Transcript Variant: This variant (4) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon, compared to variant 1. The encoded protein (isoform 4) is shorter than isoform 1. This variant corresponds to the mouse APEG-1 variant described in PubMed ID: 10973969. ##Evidence-Data-START## Transcript exon combination :: AL529926.3, AL709990.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCCCA GTCCCAGCCA GAACCGCCGT TCTTCTGACA CTGGCTCCAA GGCACCCCCC 
ACCTTCAAGG TCTCACTTAT GGACCAGTCA GTAAGAGAAG GCCAAGATGT CATCATGAGC 
ATCCGCGTGC AGGGGGAGCC CAAGCCTGTG GTCTCCTGGC TGAGAAACCG CCAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCGGAGGAG GCTGAGGGTG GGCTGTGCCG GCTGCGGATC 
CTGGCTGCAG AGCGTGGCGA TGCTGGTTTC TACACTTGCA AAGCGGTCAA TGAGTATGGT 
GCTCGGCAGT GCGAGGCCCG CTTGGAGGTC CGAGGCGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166947.1
CDS	293..634
Translation	MKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	NM_001173476.1
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001173476.1

ORF Insert Sequence:

ATGAAGCCCA GTCCCAGCCA GAACCGCCGT TCTTCTGACA CTGGCTCCAA GGCACCCCCC 
ACCTTCAAGG TCTCACTTAT GGACCAGTCA GTAAGAGAAG GCCAAGATGT CATCATGAGC 
ATCCGCGTGC AGGGGGAGCC CAAGCCTGTG GTCTCCTGGC TGAGAAACCG CCAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCGGAGGAG GCTGAGGGTG GGCTGTGCCG GCTGCGGATC 
CTGGCTGCAG AGCGTGGCGA TGCTGGTTTC TACACTTGCA AAGCGGTCAA TGAGTATGGT 
GCTCGGCAGT GCGAGGCCCG CTTGGAGGTC CGAGGCGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( NM_001173476.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166947.1 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu01307
Clone ID Related Accession (Same CDS sequence)	XM_024452526.1 , NM_001173476.1 , NM_001173476.2
Accession Version	NM_001173476.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA063220.1, AC053503.7 and BM677138.1. On May 31, 2019 this sequence version replaced NM_001173476.1. Transcript Variant: This variant (4) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon, compared to variant 1. The encoded protein (isoform 4) is shorter than isoform 1. This variant corresponds to the mouse APEG-1 variant described in PubMed ID: 10973969. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.725774.1, SRR3476690.792812.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCCCA GTCCCAGCCA GAACCGCCGT TCTTCTGACA CTGGCTCCAA GGCACCCCCC 
ACCTTCAAGG TCTCACTTAT GGACCAGTCA GTAAGAGAAG GCCAAGATGT CATCATGAGC 
ATCCGCGTGC AGGGGGAGCC CAAGCCTGTG GTCTCCTGGC TGAGAAACCG CCAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCGGAGGAG GCTGAGGGTG GGCTGTGCCG GCTGCGGATC 
CTGGCTGCAG AGCGTGGCGA TGCTGGTTTC TACACTTGCA AAGCGGTCAA TGAGTATGGT 
GCTCGGCAGT GCGAGGCCCG CTTGGAGGTC CGAGGCGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166947.1
CDS	293..634
Translation	MKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	NM_001173476.2
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001173476.2

ORF Insert Sequence:

ATGAAGCCCA GTCCCAGCCA GAACCGCCGT TCTTCTGACA CTGGCTCCAA GGCACCCCCC 
ACCTTCAAGG TCTCACTTAT GGACCAGTCA GTAAGAGAAG GCCAAGATGT CATCATGAGC 
ATCCGCGTGC AGGGGGAGCC CAAGCCTGTG GTCTCCTGGC TGAGAAACCG CCAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCGGAGGAG GCTGAGGGTG GGCTGTGCCG GCTGCGGATC 
CTGGCTGCAG AGCGTGGCGA TGCTGGTTTC TACACTTGCA AAGCGGTCAA TGAGTATGGT 
GCTCGGCAGT GCGAGGCCCG CTTGGAGGTC CGAGGCGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_024452526.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308294.1 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu01307
Clone ID Related Accession (Same CDS sequence)	XM_024452526.1 , NM_001173476.1 , NM_001173476.2
Accession Version	XM_024452526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCCA GTCCCAGCCA GAACCGCCGT TCTTCTGACA CTGGCTCCAA GGCACCCCCC 
ACCTTCAAGG TCTCACTTAT GGACCAGTCA GTAAGAGAAG GCCAAGATGT CATCATGAGC 
ATCCGCGTGC AGGGGGAGCC CAAGCCTGTG GTCTCCTGGC TGAGAAACCG CCAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCGGAGGAG GCTGAGGGTG GGCTGTGCCG GCTGCGGATC 
CTGGCTGCAG AGCGTGGCGA TGCTGGTTTC TACACTTGCA AAGCGGTCAA TGAGTATGGT 
GCTCGGCAGT GCGAGGCCCG CTTGGAGGTC CGAGGCGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308294.1
CDS	1657..1998
Translation	MKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	XM_024452526.1
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452526.1

ORF Insert Sequence:

ATGAAGCCCA GTCCCAGCCA GAACCGCCGT TCTTCTGACA CTGGCTCCAA GGCACCCCCC 
ACCTTCAAGG TCTCACTTAT GGACCAGTCA GTAAGAGAAG GCCAAGATGT CATCATGAGC 
ATCCGCGTGC AGGGGGAGCC CAAGCCTGTG GTCTCCTGGC TGAGAAACCG CCAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCGGAGGAG GCTGAGGGTG GGCTGTGCCG GCTGCGGATC 
CTGGCTGCAG AGCGTGGCGA TGCTGGTTTC TACACTTGCA AAGCGGTCAA TGAGTATGGT 
GCTCGGCAGT GCGAGGCCCG CTTGGAGGTC CGAGGCGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_017003161.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858650.1 Homo sapiens SPEG complex locus (SPEG), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OHu88922
Clone ID Related Accession (Same CDS sequence)	XM_017003161.1 , XM_017003161.2
Accession Version	XM_017003161.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCG CCACCAACGA GCTGGGCCAG GCCACCTGTG CCGCCTCACT GACCGTGAGA 
CCCGGTGGGT CTACATCCCC TTTCAGCAGC CCCATCACCT CCGACGAGGA ATACCTGAGC 
CCCCCAGAGG AGTTCCCAGA GCCTGGGGAG ACCTGGCCGC GAACCCCCAC CATGAAGCCC 
AGTCCCAGCC AGAACCGCCG TTCTTCTGAC ACTGGCTCCA AGGCACCCCC CACCTTCAAG 
GTCTCACTTA TGGACCAGTC AGTAAGAGAA GGCCAAGATG TCATCATGAG CATCCGCGTG 
CAGGGGGAGC CCAAGCCTGT GGTCTCCTGG CTGAGAAACC GCCAGCCCGT GCGCCCAGAC 
CAGCGGCGCT TTGCGGAGGA GGCTGAGGGT GGGCTGTGCC GGCTGCGGAT CCTGGCTGCA 
GAGCGTGGCG ATGCTGGTTT CTACACTTGC AAAGCGGTCA ATGAGTATGG TGCTCGGCAG 
TGCGAGGCCC GCTTGGAGGT CCGAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858650.1
CDS	1899..2441
Translation	MATATNELGQATCAASLTVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGRGRGGRGDGAV

Target ORF information:

RefSeq Version	XM_017003161.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SPEG complex locus (SPEG), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003161.1

ORF Insert Sequence:

ATGGCCACCG CCACCAACGA GCTGGGCCAG GCCACCTGTG CCGCCTCACT GACCGTGAGA 
CCCGGTGGGT CTACATCCCC TTTCAGCAGC CCCATCACCT CCGACGAGGA ATACCTGAGC 
CCCCCAGAGG AGTTCCCAGA GCCTGGGGAG ACCTGGCCGC GAACCCCCAC CATGAAGCCC 
AGTCCCAGCC AGAACCGCCG TTCTTCTGAC ACTGGCTCCA AGGCACCCCC CACCTTCAAG 
GTCTCACTTA TGGACCAGTC AGTAAGAGAA GGCCAAGATG TCATCATGAG CATCCGCGTG 
CAGGGGGAGC CCAAGCCTGT GGTCTCCTGG CTGAGAAACC GCCAGCCCGT GCGCCCAGAC 
CAGCGGCGCT TTGCGGAGGA GGCTGAGGGT GGGCTGTGCC GGCTGCGGAT CCTGGCTGCA 
GAGCGTGGCG ATGCTGGTTT CTACACTTGC AAAGCGGTCA ATGAGTATGG TGCTCGGCAG 
TGCGAGGCCC GCTTGGAGGT CCGAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_017003161.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858650.1 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X15, mRNA.

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CloneID	OHu88922
Clone ID Related Accession (Same CDS sequence)	XM_017003161.1 , XM_017003161.2
Accession Version	XM_017003161.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017003161.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCG CCACCAACGA GCTGGGCCAG GCCACCTGTG CCGCCTCACT GACCGTGAGA 
CCCGGTGGGT CTACATCCCC TTTCAGCAGC CCCATCACCT CCGACGAGGA ATACCTGAGC 
CCCCCAGAGG AGTTCCCAGA GCCTGGGGAG ACCTGGCCGC GAACCCCCAC CATGAAGCCC 
AGTCCCAGCC AGAACCGCCG TTCTTCTGAC ACTGGCTCCA AGGCACCCCC CACCTTCAAG 
GTCTCACTTA TGGACCAGTC AGTAAGAGAA GGCCAAGATG TCATCATGAG CATCCGCGTG 
CAGGGGGAGC CCAAGCCTGT GGTCTCCTGG CTGAGAAACC GCCAGCCCGT GCGCCCAGAC 
CAGCGGCGCT TTGCGGAGGA GGCTGAGGGT GGGCTGTGCC GGCTGCGGAT CCTGGCTGCA 
GAGCGTGGCG ATGCTGGTTT CTACACTTGC AAAGCGGTCA ATGAGTATGG TGCTCGGCAG 
TGCGAGGCCC GCTTGGAGGT CCGAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858650.1
CDS	1669..2211
Translation	MATATNELGQATCAASLTVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGRGRGGRGDGAV

Target ORF information:

RefSeq Version	XM_017003161.2
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003161.2

ORF Insert Sequence:

ATGGCCACCG CCACCAACGA GCTGGGCCAG GCCACCTGTG CCGCCTCACT GACCGTGAGA 
CCCGGTGGGT CTACATCCCC TTTCAGCAGC CCCATCACCT CCGACGAGGA ATACCTGAGC 
CCCCCAGAGG AGTTCCCAGA GCCTGGGGAG ACCTGGCCGC GAACCCCCAC CATGAAGCCC 
AGTCCCAGCC AGAACCGCCG TTCTTCTGAC ACTGGCTCCA AGGCACCCCC CACCTTCAAG 
GTCTCACTTA TGGACCAGTC AGTAAGAGAA GGCCAAGATG TCATCATGAG CATCCGCGTG 
CAGGGGGAGC CCAAGCCTGT GGTCTCCTGG CTGAGAAACC GCCAGCCCGT GCGCCCAGAC 
CAGCGGCGCT TTGCGGAGGA GGCTGAGGGT GGGCTGTGCC GGCTGCGGAT CCTGGCTGCA 
GAGCGTGGCG ATGCTGGTTT CTACACTTGC AAAGCGGTCA ATGAGTATGG TGCTCGGCAG 
TGCGAGGCCC GCTTGGAGGT CCGAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_017003162.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858651.1 Homo sapiens SPEG complex locus (SPEG), transcript variant X16, mRNA.

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CloneID	OHu88923
Clone ID Related Accession (Same CDS sequence)	XM_017003162.1 , XM_017003162.2
Accession Version	XM_017003162.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCG CCACCAACGA GCTGGGCCAG GCCACCTGTG CCGCCTCACT GACCGTGAGA 
CCCGGTGGGT CTACATCCCC TTTCAGCAGC CCCATCACCT CCGACGAGGA ATACCTGAGC 
CCCCCAGAGG AGTTCCCAGA GCCTGGGGAG ACCTGGCCGC GAACCCCCAC CATGAAGCCC 
AGTCCCAGCC AGAACCGCCG TTCTTCTGAC ACTGGCTCCA AGGCACCCCC CACCTTCAAG 
GTCTCACTTA TGGACCAGTC AGTAAGAGAA GGCCAAGATG TCATCATGAG CATCCGCGTG 
CAGGGGGAGC CCAAGCCTGT GGTCTCCTGG CTGAGAAACC GCCAGCCCGT GCGCCCAGAC 
CAGCGGCGCT TTGCGGAGGA GGCTGAGGGT GGGCTGTGCC GGCTGCGGAT CCTGGCTGCA 
GAGCGTGGCG ATGCTGGTTT CTACACTTGC AAAGCGGTCA ATGAGTATGG TGCTCGGCAG 
TGCGAGGCCC GCTTGGAGGT CCGAGGCGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858651.1
CDS	1899..2411
Translation	MATATNELGQATCAASLTVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	XM_017003162.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SPEG complex locus (SPEG), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003162.1

ORF Insert Sequence:

ATGGCCACCG CCACCAACGA GCTGGGCCAG GCCACCTGTG CCGCCTCACT GACCGTGAGA 
CCCGGTGGGT CTACATCCCC TTTCAGCAGC CCCATCACCT CCGACGAGGA ATACCTGAGC 
CCCCCAGAGG AGTTCCCAGA GCCTGGGGAG ACCTGGCCGC GAACCCCCAC CATGAAGCCC 
AGTCCCAGCC AGAACCGCCG TTCTTCTGAC ACTGGCTCCA AGGCACCCCC CACCTTCAAG 
GTCTCACTTA TGGACCAGTC AGTAAGAGAA GGCCAAGATG TCATCATGAG CATCCGCGTG 
CAGGGGGAGC CCAAGCCTGT GGTCTCCTGG CTGAGAAACC GCCAGCCCGT GCGCCCAGAC 
CAGCGGCGCT TTGCGGAGGA GGCTGAGGGT GGGCTGTGCC GGCTGCGGAT CCTGGCTGCA 
GAGCGTGGCG ATGCTGGTTT CTACACTTGC AAAGCGGTCA ATGAGTATGG TGCTCGGCAG 
TGCGAGGCCC GCTTGGAGGT CCGAGGCGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_017003162.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858651.1 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X16, mRNA.

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CloneID	OHu88923
Clone ID Related Accession (Same CDS sequence)	XM_017003162.1 , XM_017003162.2
Accession Version	XM_017003162.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017003162.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCG CCACCAACGA GCTGGGCCAG GCCACCTGTG CCGCCTCACT GACCGTGAGA 
CCCGGTGGGT CTACATCCCC TTTCAGCAGC CCCATCACCT CCGACGAGGA ATACCTGAGC 
CCCCCAGAGG AGTTCCCAGA GCCTGGGGAG ACCTGGCCGC GAACCCCCAC CATGAAGCCC 
AGTCCCAGCC AGAACCGCCG TTCTTCTGAC ACTGGCTCCA AGGCACCCCC CACCTTCAAG 
GTCTCACTTA TGGACCAGTC AGTAAGAGAA GGCCAAGATG TCATCATGAG CATCCGCGTG 
CAGGGGGAGC CCAAGCCTGT GGTCTCCTGG CTGAGAAACC GCCAGCCCGT GCGCCCAGAC 
CAGCGGCGCT TTGCGGAGGA GGCTGAGGGT GGGCTGTGCC GGCTGCGGAT CCTGGCTGCA 
GAGCGTGGCG ATGCTGGTTT CTACACTTGC AAAGCGGTCA ATGAGTATGG TGCTCGGCAG 
TGCGAGGCCC GCTTGGAGGT CCGAGGCGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858651.1
CDS	1669..2181
Translation	MATATNELGQATCAASLTVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	XM_017003162.2
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003162.2

ORF Insert Sequence:

ATGGCCACCG CCACCAACGA GCTGGGCCAG GCCACCTGTG CCGCCTCACT GACCGTGAGA 
CCCGGTGGGT CTACATCCCC TTTCAGCAGC CCCATCACCT CCGACGAGGA ATACCTGAGC 
CCCCCAGAGG AGTTCCCAGA GCCTGGGGAG ACCTGGCCGC GAACCCCCAC CATGAAGCCC 
AGTCCCAGCC AGAACCGCCG TTCTTCTGAC ACTGGCTCCA AGGCACCCCC CACCTTCAAG 
GTCTCACTTA TGGACCAGTC AGTAAGAGAA GGCCAAGATG TCATCATGAG CATCCGCGTG 
CAGGGGGAGC CCAAGCCTGT GGTCTCCTGG CTGAGAAACC GCCAGCCCGT GCGCCCAGAC 
CAGCGGCGCT TTGCGGAGGA GGCTGAGGGT GGGCTGTGCC GGCTGCGGAT CCTGGCTGCA 
GAGCGTGGCG ATGCTGGTTT CTACACTTGC AAAGCGGTCA ATGAGTATGG TGCTCGGCAG 
TGCGAGGCCC GCTTGGAGGT CCGAGGCGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( NM_005876.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005867.3 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant 1, mRNA.

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CloneID	OHu24274
Clone ID Related Accession (Same CDS sequence)	NM_005876.5 , NM_005876.4
Accession Version	NM_005876.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC053503.7. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_005876.4. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). This variant corresponds to the mouse SPEGbeta variant described in PubMed ID: 10973969. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology MANE Ensembl match :: ENST00000312358.12/ ENSP00000311684.7 RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GATGCGGGCA CGAGGGCACC CCCCAGCCCC 
GGAGTGCCCC CGAAAAGGGC CAAGGTGGGG GCCGGCGGCG GGGCTCCTGT GGCCGTGGCC 
GGGGCGCCAG TCTTCCTGCG GCCCCTGAAG AACGCGGCGG TGTGCGCGGG CAGCGACGTG 
CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCCGCTGGTT CCGGGATGGG 
CAGCTCCTGC CCGCGCCGGC CCCCGAGCCC AGCTGCCTGT GGCTGCGGCG CTGCGGGGCG 
CAGGACGCCG GCGTGTACAG CTGCATGGCC CAGAACGAGC GGGGCCGGGC CTCCTGCGAG 
GCGGTGCTCA CAGTGCTGGA GGTCGGAGAC TCAGAGACGG CTGAGGATGA CATCAGCGAT 
GTGCAGGGAA CCCAGCGCCT GGAGCTTCGG GATGACGGGG CCTTCAGCAC CCCCACGGGG 
GGTTCTGACA CCCTGGTGGG CACCTCCCTG GACACACCCC CGACCTCCGT GACAGGCACC 
TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC 
AGTGGGGGTG GCACCCGCCG CCTCCCGGGC AGCCCAAGGC AAGCACAGGC AACCGGGGCC 
GGGCCACGGC ACCTGGGGGT GGAGCCGCTG GTGCGGGCAT CTCGAGCTAA TCTGGTGGGC 
GCAAGCTGGG GGTCAGAGGA TAGCCTTTCC GTGGCCAGTG ACCTGTACGG CAGCGCATTC 
AGCCTGTACA GAGGACGGGC GCTCTCTATC CACGTCAGCG TCCCTCAGAG CGGGTTGCGC 
AGGGAGGAGC CCGACCTTCA GCCTCAACTG GCCAGCGAAG CCCCACGCCG CCCTGCCCAG 
CCGCCTCCTT CCAAATCCGC GCTGCTCCCC CCACCGTCCC CTCGGGTCGG GAAGCGGTCC 
CCGCCGGGAC CCCCGGCCCA GCCCGCGGCC ACCCCCACGT CGCCCCACCG TCGCACTCAG 
GAGCCTGTGC TGCCCGAGGA CACCACCACC GAAGAGAAGC GAGGGAAGAA GTCCAAGTCG 
TCCGGGCCCT CCCTGGCGGG CACCGCGGAA TCCCGACCCC AGACGCCACT GAGCGAGGCC 
TCAGGCCGCC TGTCGGCGTT GGGCCGATCG CCTAGGCTGG TGCGCGCCGG CTCCCGCATC 
CTGGACAAGC TGCAGTTCTT CGAGGAGCGA CGGCGCAGCC TGGAGCGCAG CGACTCGCCG 
CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG CGCAAGGCCC GCTCTCTGGA GCAGCCCAAG 
TCGGAGCGCG GCGCACCGTG GGGCACCCCC GGGGCCTCGC AGGAAGAACT GCGGGCGCCA 
GGCAGCGTGG CCGAGCGGCG CCGCCTGTTC CAGCAGAAAG CGGCCTCGCT GGACGAGCGC 
ACGCGTCAGC GCAGCCCGGC CTCAGACCTC GAGCTGCGCT TCGCCCAGGA GCTGGGCCGC 
ATCCGCCGCT CCACGTCGCG GGAGGAGCTG GTGCGCTCGC ACGAGTCCCT GCGCGCCACG 
CTGCAGCGTG CCCCATCCCC TCGAGAGCCC GGCGAGCCCC CGCTCTTCTC TCGGCCCTCC 
ACCCCCAAGA CATCGCGGGC CGTGAGCCCC GCCGCCGCCC AGCCGCCCTC TCCGAGCAGC 
GCGGAGAAGC CGGGGGACGA GCCTGGGAGG CCCAGGAGCC GCGGGCCGGC GGGCAGGACA 
GAGCCGGGGG AAGGCCCGCA GCAGGAGGTT AGGCGTCGGG ACCAATTCCC GCTGACCCGG 
AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT GTGCCGCCCC CCGCCGCCGA TCCCCCAGAG 
GCCAGGACGA AAGCACCCCC CGGTCGGAAG CGGGAGCCCC CGGCGCAGGC CGTGCGCTTC 
CTGCCCTGGG CCACGCCGGG CCTGGAGGGC GCTGCTGTAC CCCAGACCTT GGAGAAGAAC 
AGGGCGGGGC CTGAGGCAGA GAAGAGGCTT CGCAGAGGGC CGGAGGAGGA CGGTCCCTGG 
GGGCCCTGGG ACCGCCGAGG GGCCCGCAGC CAGGGCAAAG GTCGCCGGGC CCGGCCCACC 
TCCCCTGAGC TCGAGTCTTC GGATGACTCC TACGTGTCCG CTGGAGAAGA GCCCCTAGAG 
GCCCCTGTGT TTGAGATCCC CCTGCAGAAT GTGGTGGTGG CACCAGGGGC AGATGTGCTG 
CTCAAGTGTA TCATCACTGC CAACCCCCCG CCCCAAGTGT CCTGGCACAA GGATGGGTCA 
GCGCTGCGCA GCGAGGGCCG CCTCCTCCTC CGGGCTGAGG GTGAGCGGCA CACCCTGCTG 
CTCAGGGAGG CCAGGGCAGC AGATGCCGGG AGCTATATGG CCACCGCCAC CAACGAGCTG 
GGCCAGGCCA CCTGTGCCGC CTCACTGACC GTGAGACCCG GTGGGTCTAC ATCCCCTTTC 
AGCAGCCCCA TCACCTCCGA CGAGGAATAC CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT 
GGGGAGACCT GGCCGCGAAC CCCCACCATG AAGCCCAGTC CCAGCCAGAA CCGCCGTTCT 
TCTGACACTG GCTCCAAGGC ACCCCCCACC TTCAAGGTCT CACTTATGGA CCAGTCAGTA 
AGAGAAGGCC AAGATGTCAT CATGAGCATC CGCGTGCAGG GGGAGCCCAA GCCTGTGGTC 
TCCTGGCTGA GAAACCGCCA GCCCGTGCGC CCAGACCAGC GGCGCTTTGC GGAGGAGGCT 
GAGGGTGGGC TGTGCCGGCT GCGGATCCTG GCTGCAGAGC GTGGCGATGC TGGTTTCTAC 
ACTTGCAAAG CGGTCAATGA GTATGGTGCT CGGCAGTGCG AGGCCCGCTT GGAGGTCCGA 
GCACACCCTG AAAGCCGGTC CCTGGCCGTG CTGGCCCCCC TGCAGGACGT GGACGTGGGG 
GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG GTGGCGGGGC CCACTGACGT GGAGGTGGAT 
TGGCTGTGCC GTGGCCGCCT GCTGCAGCCT GCACTGCTCA AATGCAAGAT GCATTTCGAT 
GGCCGCAAAT GCAAGCTGCT ACTTACATCT GTACATGAGG ACGACAGTGG CGTCTACACC 
TGCAAGCTCA GCACGGCCAA AGATGAGCTG ACCTGCAGTG CCCGGCTGAC CGTGCGGCCC 
TCGTTGGCAC CCCTGTTCAC ACGGCTGCTG GAAGATGTGG AGGTGTTGGA GGGCCGAGCT 
GCCCGTTTCG ACTGCAAGAT CAGTGGCACC CCGCCCCCTG TTGTTACCTG GACTCATTTT 
GGCTGCCCCA TGGAGGAGAG TGAGAACTTG CGGCTGCGGC AGGACGGGGG TCTGCACTCA 
CTGCACATTG CCCATGTGGG CAGCGAGGAC GAGGGGCTCT ATGCGGTCAG TGCTGTTAAC 
ACCCATGGCC AGGCCCACTG CTCAGCCCAG CTGTATGTAG AAGAGCCCCG GACAGCCGCC 
TCAGGCCCCA GCTCGAAGCT GGAGAAGATG CCATCCATTC CCGAGGAGCC AGAGCAGGGT 
GAGCTGGAGC GGCTGTCCAT TCCTGACTTC CTGCGGCCAC TGCAGGACCT GGAGGTGGGA 
CTGGCCAAGG AGGCCATGCT AGAGTGCCAG GTGACCGGCC TGCCCTACCC CACCATCAGC 
TGGTTCCACA ATGGCCACCG CATCCAGAGC AGCGACGACC GGCGCATGAC ACAGTACAGG 
GATGTCCATC GCTTGGTGTT CCCTGCCGTG GGGCCTCAGC ACGCCGGTGT CTACAAGAGC 
GTCATTGCCA ACAAGCTGGG CAAAGCTGCC TGCTATGCCC ACCTGTATGT CACAGATGTG 
GTCCCAGGCC CTCCAGATGG CGCCCCGCAG GTGGTGGCTG TGACGGGGAG GATGGTCACA 
CTCACATGGA ACCCCCCCAG GAGTCTGGAC ATGGCCATCG ACCCGGACTC CCTGACGTAC 
ACAGTGCAGC ACCAGGTGCT GGGCTCGGAC CAGTGGACGG CACTGGTCAC AGGCCTGCGG 
GAGCCAGGGT GGGCAGCCAC AGGGCTGCGT AAGGGGGTCC AGCACATCTT CCGGGTCCTC 
AGCACCACTG TCAAGAGCAG CAGCAAGCCC TCACCCCCTT CTGAGCCTGT GCAGCTGCTG 
GAGCACGGCC CAACCCTGGA GGAGGCCCCT GCCATGCTGG ACAAACCAGA CATCGTGTAT 
GTGGTGGAGG GACAGCCTGC CAGCGTCACC GTCACATTCA ACCATGTGGA GGCCCAGGTC 
GTCTGGAGGA GCTGCCGAGG GGCCCTCCTA GAGGCACGGG CCGGTGTGTA CGAGCTGAGC 
CAGCCAGATG ATGACCAGTA CTGTCTTCGG ATCTGCCGGG TGAGCCGCCG GGACATGGGG 
GCCCTCACCT GCACCGCCCG AAACCGTCAC GGCACACAGA CCTGCTCGGT CACATTGGAG 
CTGGCAGAGG CCCCTCGGTT TGAGTCCATC ATGGAGGACG TGGAGGTGGG GGCTGGGGAA 
ACTGCTCGCT TTGCGGTGGT GGTCGAGGGA AAACCACTGC CGGACATCAT GTGGTACAAG 
GACGAGGTGC TGCTGACCGA GAGCAGCCAT GTGAGCTTCG TGTACGAGGA GAATGAGTGC 
TCCCTGGTGG TGCTCAGCAC GGGGGCCCAG GATGGAGGCG TCTACACCTG CACCGCCCAG 
AACCTGGCGG GTGAGGTCTC CTGCAAAGCA GAGTTGGCTG TGCATTCAGC TCAGACAGCT 
ATGGAGGTCG AGGGGGTCGG GGAGGATGAG GACCATCGAG GAAGGAGACT CAGCGACTTT 
TATGACATCC ACCAGGAGAT CGGCAGGGGT GCTTTCTCCT ACTTGCGGCG CATAGTGGAG 
CGTAGCTCCG GCCTGGAGTT TGCGGCCAAG TTCATCCCCA GCCAGGCCAA GCCAAAGGCA 
TCAGCGCGTC GGGAGGCCCG GCTGCTGGCC AGGCTCCAGC ACGACTGTGT CCTCTACTTC 
CATGAGGCCT TCGAGAGGCG CCGGGGACTG GTCATTGTCA CCGAGCTCTG CACAGAGGAG 
CTGCTGGAGC GAATCGCCAG GAAACCCACC GTGTGTGAGT CTGAGATCCG GGCCTATATG 
CGGCAGGTGC TAGAGGGAAT ACACTACCTG CACCAGAGCC ACGTGCTGCA CCTCGATGTC 
AAGCCTGAGA ACCTGCTGGT GTGGGATGGT GCTGCGGGCG AGCAGCAGGT GCGGATCTGT 
GACTTTGGGA ATGCCCAGGA GCTGACTCCA GGAGAGCCCC AGTACTGCCA GTATGGCACA 
CCTGAGTTTG TAGCACCCGA GATTGTCAAT CAGAGCCCCG TGTCTGGAGT CACTGACATC 
TGGCCTGTGG GTGTTGTTGC CTTCCTCTGT CTGACAGGAA TCTCCCCGTT TGTTGGGGAA 
AATGACCGGA CAACATTGAT GAACATCCGA AACTACAACG TGGCCTTCGA GGAGACCACA 
TTCCTGAGCC TGAGCAGGGA GGCCCGGGGC TTCCTCATCA AAGTGTTGGT GCAGGACCGG 
CTGAGACCTA CCGCAGAAGA GACCCTAGAA CATCCTTGGT TCAAAACTCA GGCAAAGGGC 
GCAGAGGTGA GCACGGATCA CCTGAAGCTA TTCCTCTCCC GGCGGAGGTG GCAGCGCTCC 
CAGATCAGCT ACAAATGCCA CCTGGTGCTG CGCCCCATCC CCGAGCTGCT GCGGGCCCCC 
CCAGAGCGGG TGTGGGTGAC CATGCCCAGA AGGCCACCCC CCAGTGGGGG GCTCTCATCC 
TCCTCGGATT CTGAAGAGGA AGAGCTGGAA GAGCTGCCCT CAGTGCCCCG CCCACTGCAG 
CCCGAGTTCT CTGGCTCCCG GGTGTCCCTC ACAGACATTC CCACTGAGGA TGAGGCCCTG 
GGGACCCCAG AGACTGGGGC TGCCACCCCC ATGGACTGGC AGGAGCAGGG AAGGGCTCCC 
TCTCAGGACC AGGAGGCTCC CAGCCCAGAG GCCCTCCCCT CCCCAGGCCA GGAGCCCGCA 
GCTGGGGCTA GCCCCAGGCG GGGAGAGCTC CGCAGGGGCA GCTCGGCTGA GAGCGCCCTG 
CCCCGGGCCG GGCCGCGGGA GCTGGGCCGG GGCCTGCACA AGGCGGCGTC TGTGGAGCTG 
CCGCAGCGCC GGAGCCCCAG CCCGGGAGCC ACCCGCCTGG CCCGGGGAGG CCTGGGTGAG 
GGCGAGTATG CCCAGAGGCT GCAGGCCCTG CGCCAGCGGC TGCTGCGGGG AGGCCCCGAG 
GATGGCAAGG TCAGCGGCCT CAGGGGTCCC CTGCTGGAGA GCCTGGGGGG CCGTGCTCGG 
GACCCCCGGA TGGCACGAGC TGCCTCCAGC GAGGCAGCGC CCCACCACCA GCCCCCACTC 
GAGAACCGGG GCCTGCAAAA GAGCAGCAGC TTCTCCCAGG GTGAGGCGGA GCCCCGGGGC 
CGGCACCGCC GAGCGGGGGC GCCCCTCGAG ATCCCCGTGG CCAGGCTTGG GGCCCGTAGG 
CTACAGGAGT CTCCTTCCCT GTCTGCCCTC AGCGAGGCCC AGCCATCCAG CCCTGCACGG 
CCCAGCGCCC CCAAACCCAG TACCCCTAAG TCTGCAGAAC CTTCTGCCAC CACACCTAGT 
GATGCTCCGC AGCCCCCCGC ACCCCAGCCT GCCCAAGACA AGGCTCCAGA GCCCAGGCCA 
GAACCAGTCC GAGCCTCCAA GCCTGCACCA CCCCCCCAGG CCCTGCAAAC CCTAGCGCTG 
CCCCTCACAC CCTATGCTCA GATCATTCAG TCCCTCCAGC TGTCAGGCCA CGCCCAGGGC 
CCCTCGCAGG GCCCTGCCGC GCCGCCTTCA GAGCCCAAGC CCCACGCTGC TGTCTTTGCC 
AGGGTGGCCT CCCCACCTCC GGGAGCCCCC GAGAAGCGCG TGCCCTCAGC CGGGGGTCCC 
CCGGTGCTAG CCGAGAAAGC CCGAGTTCCC ACGGTGCCCC CCAGGCCAGG CAGCAGTCTC 
AGTAGCAGCA TCGAAAACTT GGAGTCGGAG GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC 
CGCGAGTCGC CCCTGTCGCT GGGGCTGCGG CTGCTGAGCC GTTCGCGCTC GGAGGAGCGC 
GGCCCCTTCC GTGGGGCCGA GGAGGAGGAT GGCATATACC GGCCCAGCCC GGCGGGGACC 
CCGCTGGAGC TGGTGCGACG GCCTGAGCGC TCACGCTCGG TGCAGGACCT CAGGGCTGTC 
GGAGAGCCTG GCCTCGTCCG CCGCCTCTCG CTGTCACTGT CCCAGCGGCT GCGGCGGACC 
CCTCCCGCGC AGCGCCACCC GGCCTGGGAG GCCCGCGGCG GGGACGGAGA GAGCTCGGAG 
GGCGGGAGCT CGGCGCGGGG CTCCCCGGTG CTGGCGATGC GCAGGCGGCT GAGCTTCACC 
CTGGAGCGGC TGTCCAGCCG ATTGCAGCGC AGTGGCAGCA GCGAGGACTC GGGGGGCGCG 
TCGGGCCGCA GCACGCCGCT GTTCGGACGG CTTCGCAGGG CCACGTCCGA GGGCGAGAGT 
CTGCGGCGCC TTGGCCTTCC GCACAACCAG TTGGCCGCCC AGGCCGGCGC CACCACGCCT 
TCCGCCGAGT CCCTGGGCTC CGAGGCCAGC GCCACGTCGG GCTCCTCAGC CCCAGGGGAA 
AGCCGAAGCC GGCTCCGCTG GGGCTTCTCT CGGCCGCGGA AGGACAAGGG GTTATCGCCA 
CCAAACCTCT CTGCCAGCGT CCAGGAGGAG TTGGGTCACC AGTACGTGCG CAGTGAGTCA 
GACTTCCCCC CAGTCTTCCA CATCAAACTC AAGGACCAGG TGCTGCTGGA GGGGGAGGCA 
GCCACCCTGC TCTGCCTGCC AGCGGCCTGC CCTGCACCGC ACATCTCCTG GATGAAAGAC 
AAGAAGTCCT TGAGGTCAGA GCCCTCAGTG ATCATCGTGT CCTGCAAAGA TGGGCGGCAG 
CTGCTCAGCA TCCCCCGGGC GGGCAAGCGG CACGCCGGTC TCTATGAGTG CTCGGCCACC 
AACGTACTGG GCAGCATCAC CAGCTCCTGT ACCGTGGCTG TGGCCCGAGT CCCAGGAAAG 
CTAGCTCCTC CAGAGGTACC CCAGACCTAC CAGGACACGG CGCTGGTGCT GTGGAAGCCG 
GGAGACAGCC GGGCACCTTG CACGTATACG CTGGAGCGGC GAGTGGATGG GGAGTCTGTG 
TGGCACCCTG TGAGCTCAGG CATCCCCGAC TGTTACTACA ACGTGACCCA CCTGCCAGTT 
GGCGTGACTG TGAGGTTCCG TGTGGCCTGT GCCAACCGTG CTGGGCAGGG GCCCTTCAGC 
AACTCTTCTG AGAAGGTCTT TGTCAGGGGT ACTCAAGATT CTTCAGCTGT GCCATCTGCT 
GCCCACCAAG AGGCCCCTGT CACCTCAAGG CCAGCCAGGG CCCGGCCTCC TGACTCTCCT 
ACCTCACTGG CCCCACCCCT AGCTCCTGCT GCCCCCACAC CCCCGTCAGT CACTGTCAGC 
CCCTCATCTC CCCCCACACC TCCTAGCCAG GCCTTGTCCT CGCTCAAGGC TGTGGGTCCA 
CCACCCCAAA CCCCTCCACG AAGACACAGG GGCCTGCAGG CTGCCCGGCC AGCGGAGCCC 
ACCCTACCCA GTACCCACGT CACCCCAAGT GAGCCCAAGC CTTTCGTCCT TGACACTGGG 
ACCCCGATCC CAGCCTCCAC TCCTCAAGGG GTTAAACCAG TGTCTTCCTC TACTCCTGTG 
TATGTGGTGA CTTCCTTTGT GTCTGCACCA CCAGCCCCTG AGCCCCCAGC CCCTGAGCCC 
CCTCCTGAGC CTACCAAGGT GACTGTGCAG AGCCTCAGCC CGGCCAAGGA GGTGGTCAGC 
TCCCCTGGGA GCAGTCCCCG AAGCTCTCCC AGGCCTGAGG GTACCACTCT TCGACAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAAGCCA GGGGCCGCTT TGGTGTTGTG 
CGAGCGTGCC GGGAGAATGC CACGGGGCGA ACGTTCGTGG CCAAGATCGT GCCCTATGCT 
GCCGAGGGCA AGCGGCGGGT CCTGCAGGAG TACGAGGTGC TGCGGACCCT GCACCACGAG 
CGGATCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT CATTGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGTGACA GGTTCCGGTA TTCTGAGGAT 
GACGTGGCCA CTTACATGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTCCACC TAGACATCAA GCCAGACAAC CTGCTGCTGG CCCCTGACAA TGCCCTCAAG 
ATTGTGGACT TTGGCAGTGC CCAGCCCTAC AACCCCCAGG CCCTTAGGCC CCTTGGCCAC 
CGCACGGGCA CGCTGGAGTT CATGGCTCCG GAGATGGTGA AGGGAGAACC CATCGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACTTACATTA TGCTCAGTGG ACGCTCCCCG 
TTCTATGAGC CAGACCCCCA GGAAACGGAG GCTCGGATTG TGGGGGGCCG CTTTGATGCC 
TTCCAGCTGT ACCCCAATAC ATCCCAGAGC GCCACCCTCT TCTTGCGAAA GGTTCTCTCT 
GTACATCCCT GGAGCCGGCC CTCCCTGCAG GACTGCCTGG CCCACCCATG GTTGCAGGAC 
GCCTACCTGA TGAAGCTGCG CCGCCAGACG CTCACCTTCA CCACCAACCG GCTCAAGGAG 
TTCCTGGGCG AGCAGCGGCG GCGCCGGGCT GAGGCTGCCA CCCGCCACAA GGTGCTGCTG 
CGCTCCTACC CTGGCGGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005867.3
CDS	136..9939
Translation	MQKARGTRGEDAGTRAPPSPGVPPKRAKVGAGGGAPVAVAGAPVFLRPLKNAAVCAGSDVRLRVVVSGTPQPSLRWFRDGQLLPAPAPEPSCLWLRRCGAQDAGVYSCMAQNERGRASCEAVLTVLEVGDSETAEDDISDVQGTQRLELRDDGAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRRLPGSPRQAQATGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSVPQSGLRREEPDLQPQLASEAPRRPAQPPPSKSALLPPPSPRVGKRSPPGPPAQPAATPTSPHRRTQEPVLPEDTTTEEKRGKKSKSSGPSLAGTAESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSERGAPWGTPGASQEELRAPGSVAERRRLFQQKAASLDERTRQRSPASDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAAAQPPSPSSAEKPGDEPGRPRSRGPAGRTEPGEGPQQEVRRRDQFPLTRSRAIQECRSPVPPPAADPPEARTKAPPGRKREPPAQAVRFLPWATPGLEGAAVPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGARSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNVVVAPGADVLLKCIITANPPPQVSWHKDGSALRSEGRLLLRAEGERHTLLLREARAADAGSYMATATNELGQATCAASLTVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPTDVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARFDCKISGTPPPVVTWTHFGCPMEESENLRLRQDGGLHSLHIAHVGSEDEGLYAVSAVNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEQGELERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDVHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPQVVAVTGRMVTLTWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPGWAATGLRKGVQHIFRVLSTTVKSSSKPSPPSEPVQLLEHGPTLEEAPAMLDKPDIVYVVEGQPASVTVTFNHVEAQVVWRSCRGALLEARAGVYELSQPDDDQYCLRICRVSRRDMGALTCTARNRHGTQTCSVTLELAEAPRFESIMEDVEVGAGETARFAVVVEGKPLPDIMWYKDEVLLTESSHVSFVYEENECSLVVLSTGAQDGGVYTCTAQNLAGEVSCKAELAVHSAQTAMEVEGVGEDEDHRGRRLSDFYDIHQEIGRGAFSYLRRIVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHDCVLYFHEAFERRRGLVIVTELCTEELLERIARKPTVCESEIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTQAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGELRRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQPPAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPSEPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEAKFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAVGEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAESLGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPHISWMKDKKSLRSEPSVIIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARVPGKLAPPEVPQTYQDTALVLWKPGDSRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTHLPVGVTVRFRVACANRAGQGPFSNSSEKVFVRGTQDSSAVPSAAHQEAPVTSRPARARPPDSPTSLAPPLAPAAPTPPSVTVSPSSPPTPPSQALSSLKAVGPPPQTPPRRHRGLQAARPAEPTLPSTHVTPSEPKPFVLDTGTPIPASTPQGVKPVSSSTPVYVVTSFVSAPPAPEPPAPEPPPEPTKVTVQSLSPAKEVVSSPGSSPRSSPRPEGTTLRQGPPQKPYTFLEEKARGRFGVVRACRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERIMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYMVQLLQGLDYLHGHHVLHLDIKPDNLLLAPDNALKIVDFGSAQPYNPQALRPLGHRTGTLEFMAPEMVKGEPIGSATDIWGAGVLTYIMLSGRSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGGP

Target ORF information:

RefSeq Version	NM_005876.5
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005876.5

ORF Insert Sequence:

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GATGCGGGCA CGAGGGCACC CCCCAGCCCC 
GGAGTGCCCC CGAAAAGGGC CAAGGTGGGG GCCGGCGGCG GGGCTCCTGT GGCCGTGGCC 
GGGGCGCCAG TCTTCCTGCG GCCCCTGAAG AACGCGGCGG TGTGCGCGGG CAGCGACGTG 
CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCCGCTGGTT CCGGGATGGG 
CAGCTCCTGC CCGCGCCGGC CCCCGAGCCC AGCTGCCTGT GGCTGCGGCG CTGCGGGGCG 
CAGGACGCCG GCGTGTACAG CTGCATGGCC CAGAACGAGC GGGGCCGGGC CTCCTGCGAG 
GCGGTGCTCA CAGTGCTGGA GGTCGGAGAC TCAGAGACGG CTGAGGATGA CATCAGCGAT 
GTGCAGGGAA CCCAGCGCCT GGAGCTTCGG GATGACGGGG CCTTCAGCAC CCCCACGGGG 
GGTTCTGACA CCCTGGTGGG CACCTCCCTG GACACACCCC CGACCTCCGT GACAGGCACC 
TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC 
AGTGGGGGTG GCACCCGCCG CCTCCCGGGC AGCCCAAGGC AAGCACAGGC AACCGGGGCC 
GGGCCACGGC ACCTGGGGGT GGAGCCGCTG GTGCGGGCAT CTCGAGCTAA TCTGGTGGGC 
GCAAGCTGGG GGTCAGAGGA TAGCCTTTCC GTGGCCAGTG ACCTGTACGG CAGCGCATTC 
AGCCTGTACA GAGGACGGGC GCTCTCTATC CACGTCAGCG TCCCTCAGAG CGGGTTGCGC 
AGGGAGGAGC CCGACCTTCA GCCTCAACTG GCCAGCGAAG CCCCACGCCG CCCTGCCCAG 
CCGCCTCCTT CCAAATCCGC GCTGCTCCCC CCACCGTCCC CTCGGGTCGG GAAGCGGTCC 
CCGCCGGGAC CCCCGGCCCA GCCCGCGGCC ACCCCCACGT CGCCCCACCG TCGCACTCAG 
GAGCCTGTGC TGCCCGAGGA CACCACCACC GAAGAGAAGC GAGGGAAGAA GTCCAAGTCG 
TCCGGGCCCT CCCTGGCGGG CACCGCGGAA TCCCGACCCC AGACGCCACT GAGCGAGGCC 
TCAGGCCGCC TGTCGGCGTT GGGCCGATCG CCTAGGCTGG TGCGCGCCGG CTCCCGCATC 
CTGGACAAGC TGCAGTTCTT CGAGGAGCGA CGGCGCAGCC TGGAGCGCAG CGACTCGCCG 
CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG CGCAAGGCCC GCTCTCTGGA GCAGCCCAAG 
TCGGAGCGCG GCGCACCGTG GGGCACCCCC GGGGCCTCGC AGGAAGAACT GCGGGCGCCA 
GGCAGCGTGG CCGAGCGGCG CCGCCTGTTC CAGCAGAAAG CGGCCTCGCT GGACGAGCGC 
ACGCGTCAGC GCAGCCCGGC CTCAGACCTC GAGCTGCGCT TCGCCCAGGA GCTGGGCCGC 
ATCCGCCGCT CCACGTCGCG GGAGGAGCTG GTGCGCTCGC ACGAGTCCCT GCGCGCCACG 
CTGCAGCGTG CCCCATCCCC TCGAGAGCCC GGCGAGCCCC CGCTCTTCTC TCGGCCCTCC 
ACCCCCAAGA CATCGCGGGC CGTGAGCCCC GCCGCCGCCC AGCCGCCCTC TCCGAGCAGC 
GCGGAGAAGC CGGGGGACGA GCCTGGGAGG CCCAGGAGCC GCGGGCCGGC GGGCAGGACA 
GAGCCGGGGG AAGGCCCGCA GCAGGAGGTT AGGCGTCGGG ACCAATTCCC GCTGACCCGG 
AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT GTGCCGCCCC CCGCCGCCGA TCCCCCAGAG 
GCCAGGACGA AAGCACCCCC CGGTCGGAAG CGGGAGCCCC CGGCGCAGGC CGTGCGCTTC 
CTGCCCTGGG CCACGCCGGG CCTGGAGGGC GCTGCTGTAC CCCAGACCTT GGAGAAGAAC 
AGGGCGGGGC CTGAGGCAGA GAAGAGGCTT CGCAGAGGGC CGGAGGAGGA CGGTCCCTGG 
GGGCCCTGGG ACCGCCGAGG GGCCCGCAGC CAGGGCAAAG GTCGCCGGGC CCGGCCCACC 
TCCCCTGAGC TCGAGTCTTC GGATGACTCC TACGTGTCCG CTGGAGAAGA GCCCCTAGAG 
GCCCCTGTGT TTGAGATCCC CCTGCAGAAT GTGGTGGTGG CACCAGGGGC AGATGTGCTG 
CTCAAGTGTA TCATCACTGC CAACCCCCCG CCCCAAGTGT CCTGGCACAA GGATGGGTCA 
GCGCTGCGCA GCGAGGGCCG CCTCCTCCTC CGGGCTGAGG GTGAGCGGCA CACCCTGCTG 
CTCAGGGAGG CCAGGGCAGC AGATGCCGGG AGCTATATGG CCACCGCCAC CAACGAGCTG 
GGCCAGGCCA CCTGTGCCGC CTCACTGACC GTGAGACCCG GTGGGTCTAC ATCCCCTTTC 
AGCAGCCCCA TCACCTCCGA CGAGGAATAC CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT 
GGGGAGACCT GGCCGCGAAC CCCCACCATG AAGCCCAGTC CCAGCCAGAA CCGCCGTTCT 
TCTGACACTG GCTCCAAGGC ACCCCCCACC TTCAAGGTCT CACTTATGGA CCAGTCAGTA 
AGAGAAGGCC AAGATGTCAT CATGAGCATC CGCGTGCAGG GGGAGCCCAA GCCTGTGGTC 
TCCTGGCTGA GAAACCGCCA GCCCGTGCGC CCAGACCAGC GGCGCTTTGC GGAGGAGGCT 
GAGGGTGGGC TGTGCCGGCT GCGGATCCTG GCTGCAGAGC GTGGCGATGC TGGTTTCTAC 
ACTTGCAAAG CGGTCAATGA GTATGGTGCT CGGCAGTGCG AGGCCCGCTT GGAGGTCCGA 
GCACACCCTG AAAGCCGGTC CCTGGCCGTG CTGGCCCCCC TGCAGGACGT GGACGTGGGG 
GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG GTGGCGGGGC CCACTGACGT GGAGGTGGAT 
TGGCTGTGCC GTGGCCGCCT GCTGCAGCCT GCACTGCTCA AATGCAAGAT GCATTTCGAT 
GGCCGCAAAT GCAAGCTGCT ACTTACATCT GTACATGAGG ACGACAGTGG CGTCTACACC 
TGCAAGCTCA GCACGGCCAA AGATGAGCTG ACCTGCAGTG CCCGGCTGAC CGTGCGGCCC 
TCGTTGGCAC CCCTGTTCAC ACGGCTGCTG GAAGATGTGG AGGTGTTGGA GGGCCGAGCT 
GCCCGTTTCG ACTGCAAGAT CAGTGGCACC CCGCCCCCTG TTGTTACCTG GACTCATTTT 
GGCTGCCCCA TGGAGGAGAG TGAGAACTTG CGGCTGCGGC AGGACGGGGG TCTGCACTCA 
CTGCACATTG CCCATGTGGG CAGCGAGGAC GAGGGGCTCT ATGCGGTCAG TGCTGTTAAC 
ACCCATGGCC AGGCCCACTG CTCAGCCCAG CTGTATGTAG AAGAGCCCCG GACAGCCGCC 
TCAGGCCCCA GCTCGAAGCT GGAGAAGATG CCATCCATTC CCGAGGAGCC AGAGCAGGGT 
GAGCTGGAGC GGCTGTCCAT TCCTGACTTC CTGCGGCCAC TGCAGGACCT GGAGGTGGGA 
CTGGCCAAGG AGGCCATGCT AGAGTGCCAG GTGACCGGCC TGCCCTACCC CACCATCAGC 
TGGTTCCACA ATGGCCACCG CATCCAGAGC AGCGACGACC GGCGCATGAC ACAGTACAGG 
GATGTCCATC GCTTGGTGTT CCCTGCCGTG GGGCCTCAGC ACGCCGGTGT CTACAAGAGC 
GTCATTGCCA ACAAGCTGGG CAAAGCTGCC TGCTATGCCC ACCTGTATGT CACAGATGTG 
GTCCCAGGCC CTCCAGATGG CGCCCCGCAG GTGGTGGCTG TGACGGGGAG GATGGTCACA 
CTCACATGGA ACCCCCCCAG GAGTCTGGAC ATGGCCATCG ACCCGGACTC CCTGACGTAC 
ACAGTGCAGC ACCAGGTGCT GGGCTCGGAC CAGTGGACGG CACTGGTCAC AGGCCTGCGG 
GAGCCAGGGT GGGCAGCCAC AGGGCTGCGT AAGGGGGTCC AGCACATCTT CCGGGTCCTC 
AGCACCACTG TCAAGAGCAG CAGCAAGCCC TCACCCCCTT CTGAGCCTGT GCAGCTGCTG 
GAGCACGGCC CAACCCTGGA GGAGGCCCCT GCCATGCTGG ACAAACCAGA CATCGTGTAT 
GTGGTGGAGG GACAGCCTGC CAGCGTCACC GTCACATTCA ACCATGTGGA GGCCCAGGTC 
GTCTGGAGGA GCTGCCGAGG GGCCCTCCTA GAGGCACGGG CCGGTGTGTA CGAGCTGAGC 
CAGCCAGATG ATGACCAGTA CTGTCTTCGG ATCTGCCGGG TGAGCCGCCG GGACATGGGG 
GCCCTCACCT GCACCGCCCG AAACCGTCAC GGCACACAGA CCTGCTCGGT CACATTGGAG 
CTGGCAGAGG CCCCTCGGTT TGAGTCCATC ATGGAGGACG TGGAGGTGGG GGCTGGGGAA 
ACTGCTCGCT TTGCGGTGGT GGTCGAGGGA AAACCACTGC CGGACATCAT GTGGTACAAG 
GACGAGGTGC TGCTGACCGA GAGCAGCCAT GTGAGCTTCG TGTACGAGGA GAATGAGTGC 
TCCCTGGTGG TGCTCAGCAC GGGGGCCCAG GATGGAGGCG TCTACACCTG CACCGCCCAG 
AACCTGGCGG GTGAGGTCTC CTGCAAAGCA GAGTTGGCTG TGCATTCAGC TCAGACAGCT 
ATGGAGGTCG AGGGGGTCGG GGAGGATGAG GACCATCGAG GAAGGAGACT CAGCGACTTT 
TATGACATCC ACCAGGAGAT CGGCAGGGGT GCTTTCTCCT ACTTGCGGCG CATAGTGGAG 
CGTAGCTCCG GCCTGGAGTT TGCGGCCAAG TTCATCCCCA GCCAGGCCAA GCCAAAGGCA 
TCAGCGCGTC GGGAGGCCCG GCTGCTGGCC AGGCTCCAGC ACGACTGTGT CCTCTACTTC 
CATGAGGCCT TCGAGAGGCG CCGGGGACTG GTCATTGTCA CCGAGCTCTG CACAGAGGAG 
CTGCTGGAGC GAATCGCCAG GAAACCCACC GTGTGTGAGT CTGAGATCCG GGCCTATATG 
CGGCAGGTGC TAGAGGGAAT ACACTACCTG CACCAGAGCC ACGTGCTGCA CCTCGATGTC 
AAGCCTGAGA ACCTGCTGGT GTGGGATGGT GCTGCGGGCG AGCAGCAGGT GCGGATCTGT 
GACTTTGGGA ATGCCCAGGA GCTGACTCCA GGAGAGCCCC AGTACTGCCA GTATGGCACA 
CCTGAGTTTG TAGCACCCGA GATTGTCAAT CAGAGCCCCG TGTCTGGAGT CACTGACATC 
TGGCCTGTGG GTGTTGTTGC CTTCCTCTGT CTGACAGGAA TCTCCCCGTT TGTTGGGGAA 
AATGACCGGA CAACATTGAT GAACATCCGA AACTACAACG TGGCCTTCGA GGAGACCACA 
TTCCTGAGCC TGAGCAGGGA GGCCCGGGGC TTCCTCATCA AAGTGTTGGT GCAGGACCGG 
CTGAGACCTA CCGCAGAAGA GACCCTAGAA CATCCTTGGT TCAAAACTCA GGCAAAGGGC 
GCAGAGGTGA GCACGGATCA CCTGAAGCTA TTCCTCTCCC GGCGGAGGTG GCAGCGCTCC 
CAGATCAGCT ACAAATGCCA CCTGGTGCTG CGCCCCATCC CCGAGCTGCT GCGGGCCCCC 
CCAGAGCGGG TGTGGGTGAC CATGCCCAGA AGGCCACCCC CCAGTGGGGG GCTCTCATCC 
TCCTCGGATT CTGAAGAGGA AGAGCTGGAA GAGCTGCCCT CAGTGCCCCG CCCACTGCAG 
CCCGAGTTCT CTGGCTCCCG GGTGTCCCTC ACAGACATTC CCACTGAGGA TGAGGCCCTG 
GGGACCCCAG AGACTGGGGC TGCCACCCCC ATGGACTGGC AGGAGCAGGG AAGGGCTCCC 
TCTCAGGACC AGGAGGCTCC CAGCCCAGAG GCCCTCCCCT CCCCAGGCCA GGAGCCCGCA 
GCTGGGGCTA GCCCCAGGCG GGGAGAGCTC CGCAGGGGCA GCTCGGCTGA GAGCGCCCTG 
CCCCGGGCCG GGCCGCGGGA GCTGGGCCGG GGCCTGCACA AGGCGGCGTC TGTGGAGCTG 
CCGCAGCGCC GGAGCCCCAG CCCGGGAGCC ACCCGCCTGG CCCGGGGAGG CCTGGGTGAG 
GGCGAGTATG CCCAGAGGCT GCAGGCCCTG CGCCAGCGGC TGCTGCGGGG AGGCCCCGAG 
GATGGCAAGG TCAGCGGCCT CAGGGGTCCC CTGCTGGAGA GCCTGGGGGG CCGTGCTCGG 
GACCCCCGGA TGGCACGAGC TGCCTCCAGC GAGGCAGCGC CCCACCACCA GCCCCCACTC 
GAGAACCGGG GCCTGCAAAA GAGCAGCAGC TTCTCCCAGG GTGAGGCGGA GCCCCGGGGC 
CGGCACCGCC GAGCGGGGGC GCCCCTCGAG ATCCCCGTGG CCAGGCTTGG GGCCCGTAGG 
CTACAGGAGT CTCCTTCCCT GTCTGCCCTC AGCGAGGCCC AGCCATCCAG CCCTGCACGG 
CCCAGCGCCC CCAAACCCAG TACCCCTAAG TCTGCAGAAC CTTCTGCCAC CACACCTAGT 
GATGCTCCGC AGCCCCCCGC ACCCCAGCCT GCCCAAGACA AGGCTCCAGA GCCCAGGCCA 
GAACCAGTCC GAGCCTCCAA GCCTGCACCA CCCCCCCAGG CCCTGCAAAC CCTAGCGCTG 
CCCCTCACAC CCTATGCTCA GATCATTCAG TCCCTCCAGC TGTCAGGCCA CGCCCAGGGC 
CCCTCGCAGG GCCCTGCCGC GCCGCCTTCA GAGCCCAAGC CCCACGCTGC TGTCTTTGCC 
AGGGTGGCCT CCCCACCTCC GGGAGCCCCC GAGAAGCGCG TGCCCTCAGC CGGGGGTCCC 
CCGGTGCTAG CCGAGAAAGC CCGAGTTCCC ACGGTGCCCC CCAGGCCAGG CAGCAGTCTC 
AGTAGCAGCA TCGAAAACTT GGAGTCGGAG GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC 
CGCGAGTCGC CCCTGTCGCT GGGGCTGCGG CTGCTGAGCC GTTCGCGCTC GGAGGAGCGC 
GGCCCCTTCC GTGGGGCCGA GGAGGAGGAT GGCATATACC GGCCCAGCCC GGCGGGGACC 
CCGCTGGAGC TGGTGCGACG GCCTGAGCGC TCACGCTCGG TGCAGGACCT CAGGGCTGTC 
GGAGAGCCTG GCCTCGTCCG CCGCCTCTCG CTGTCACTGT CCCAGCGGCT GCGGCGGACC 
CCTCCCGCGC AGCGCCACCC GGCCTGGGAG GCCCGCGGCG GGGACGGAGA GAGCTCGGAG 
GGCGGGAGCT CGGCGCGGGG CTCCCCGGTG CTGGCGATGC GCAGGCGGCT GAGCTTCACC 
CTGGAGCGGC TGTCCAGCCG ATTGCAGCGC AGTGGCAGCA GCGAGGACTC GGGGGGCGCG 
TCGGGCCGCA GCACGCCGCT GTTCGGACGG CTTCGCAGGG CCACGTCCGA GGGCGAGAGT 
CTGCGGCGCC TTGGCCTTCC GCACAACCAG TTGGCCGCCC AGGCCGGCGC CACCACGCCT 
TCCGCCGAGT CCCTGGGCTC CGAGGCCAGC GCCACGTCGG GCTCCTCAGC CCCAGGGGAA 
AGCCGAAGCC GGCTCCGCTG GGGCTTCTCT CGGCCGCGGA AGGACAAGGG GTTATCGCCA 
CCAAACCTCT CTGCCAGCGT CCAGGAGGAG TTGGGTCACC AGTACGTGCG CAGTGAGTCA 
GACTTCCCCC CAGTCTTCCA CATCAAACTC AAGGACCAGG TGCTGCTGGA GGGGGAGGCA 
GCCACCCTGC TCTGCCTGCC AGCGGCCTGC CCTGCACCGC ACATCTCCTG GATGAAAGAC 
AAGAAGTCCT TGAGGTCAGA GCCCTCAGTG ATCATCGTGT CCTGCAAAGA TGGGCGGCAG 
CTGCTCAGCA TCCCCCGGGC GGGCAAGCGG CACGCCGGTC TCTATGAGTG CTCGGCCACC 
AACGTACTGG GCAGCATCAC CAGCTCCTGT ACCGTGGCTG TGGCCCGAGT CCCAGGAAAG 
CTAGCTCCTC CAGAGGTACC CCAGACCTAC CAGGACACGG CGCTGGTGCT GTGGAAGCCG 
GGAGACAGCC GGGCACCTTG CACGTATACG CTGGAGCGGC GAGTGGATGG GGAGTCTGTG 
TGGCACCCTG TGAGCTCAGG CATCCCCGAC TGTTACTACA ACGTGACCCA CCTGCCAGTT 
GGCGTGACTG TGAGGTTCCG TGTGGCCTGT GCCAACCGTG CTGGGCAGGG GCCCTTCAGC 
AACTCTTCTG AGAAGGTCTT TGTCAGGGGT ACTCAAGATT CTTCAGCTGT GCCATCTGCT 
GCCCACCAAG AGGCCCCTGT CACCTCAAGG CCAGCCAGGG CCCGGCCTCC TGACTCTCCT 
ACCTCACTGG CCCCACCCCT AGCTCCTGCT GCCCCCACAC CCCCGTCAGT CACTGTCAGC 
CCCTCATCTC CCCCCACACC TCCTAGCCAG GCCTTGTCCT CGCTCAAGGC TGTGGGTCCA 
CCACCCCAAA CCCCTCCACG AAGACACAGG GGCCTGCAGG CTGCCCGGCC AGCGGAGCCC 
ACCCTACCCA GTACCCACGT CACCCCAAGT GAGCCCAAGC CTTTCGTCCT TGACACTGGG 
ACCCCGATCC CAGCCTCCAC TCCTCAAGGG GTTAAACCAG TGTCTTCCTC TACTCCTGTG 
TATGTGGTGA CTTCCTTTGT GTCTGCACCA CCAGCCCCTG AGCCCCCAGC CCCTGAGCCC 
CCTCCTGAGC CTACCAAGGT GACTGTGCAG AGCCTCAGCC CGGCCAAGGA GGTGGTCAGC 
TCCCCTGGGA GCAGTCCCCG AAGCTCTCCC AGGCCTGAGG GTACCACTCT TCGACAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAAGCCA GGGGCCGCTT TGGTGTTGTG 
CGAGCGTGCC GGGAGAATGC CACGGGGCGA ACGTTCGTGG CCAAGATCGT GCCCTATGCT 
GCCGAGGGCA AGCGGCGGGT CCTGCAGGAG TACGAGGTGC TGCGGACCCT GCACCACGAG 
CGGATCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT CATTGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGTGACA GGTTCCGGTA TTCTGAGGAT 
GACGTGGCCA CTTACATGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTCCACC TAGACATCAA GCCAGACAAC CTGCTGCTGG CCCCTGACAA TGCCCTCAAG 
ATTGTGGACT TTGGCAGTGC CCAGCCCTAC AACCCCCAGG CCCTTAGGCC CCTTGGCCAC 
CGCACGGGCA CGCTGGAGTT CATGGCTCCG GAGATGGTGA AGGGAGAACC CATCGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACTTACATTA TGCTCAGTGG ACGCTCCCCG 
TTCTATGAGC CAGACCCCCA GGAAACGGAG GCTCGGATTG TGGGGGGCCG CTTTGATGCC 
TTCCAGCTGT ACCCCAATAC ATCCCAGAGC GCCACCCTCT TCTTGCGAAA GGTTCTCTCT 
GTACATCCCT GGAGCCGGCC CTCCCTGCAG GACTGCCTGG CCCACCCATG GTTGCAGGAC 
GCCTACCTGA TGAAGCTGCG CCGCCAGACG CTCACCTTCA CCACCAACCG GCTCAAGGAG 
TTCCTGGGCG AGCAGCGGCG GCGCCGGGCT GAGGCTGCCA CCCGCCACAA GGTGCTGCTG 
CGCTCCTACC CTGGCGGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( NM_005876.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005867.3 Homo sapiens SPEG complex locus (SPEG), transcript variant 1, mRNA.

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CloneID	OHu24274
Clone ID Related Accession (Same CDS sequence)	NM_005876.5 , NM_005876.4
Accession Version	NM_005876.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC053503.7. This sequence is a reference standard in the RefSeqGene project. On Oct 1, 2007 this sequence version replaced NM_005876.3. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). This variant corresponds to the mouse SPEGbeta variant described in PubMed ID: 10973969. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GATGCGGGCA CGAGGGCACC CCCCAGCCCC 
GGAGTGCCCC CGAAAAGGGC CAAGGTGGGG GCCGGCGGCG GGGCTCCTGT GGCCGTGGCC 
GGGGCGCCAG TCTTCCTGCG GCCCCTGAAG AACGCGGCGG TGTGCGCGGG CAGCGACGTG 
CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCCGCTGGTT CCGGGATGGG 
CAGCTCCTGC CCGCGCCGGC CCCCGAGCCC AGCTGCCTGT GGCTGCGGCG CTGCGGGGCG 
CAGGACGCCG GCGTGTACAG CTGCATGGCC CAGAACGAGC GGGGCCGGGC CTCCTGCGAG 
GCGGTGCTCA CAGTGCTGGA GGTCGGAGAC TCAGAGACGG CTGAGGATGA CATCAGCGAT 
GTGCAGGGAA CCCAGCGCCT GGAGCTTCGG GATGACGGGG CCTTCAGCAC CCCCACGGGG 
GGTTCTGACA CCCTGGTGGG CACCTCCCTG GACACACCCC CGACCTCCGT GACAGGCACC 
TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC 
AGTGGGGGTG GCACCCGCCG CCTCCCGGGC AGCCCAAGGC AAGCACAGGC AACCGGGGCC 
GGGCCACGGC ACCTGGGGGT GGAGCCGCTG GTGCGGGCAT CTCGAGCTAA TCTGGTGGGC 
GCAAGCTGGG GGTCAGAGGA TAGCCTTTCC GTGGCCAGTG ACCTGTACGG CAGCGCATTC 
AGCCTGTACA GAGGACGGGC GCTCTCTATC CACGTCAGCG TCCCTCAGAG CGGGTTGCGC 
AGGGAGGAGC CCGACCTTCA GCCTCAACTG GCCAGCGAAG CCCCACGCCG CCCTGCCCAG 
CCGCCTCCTT CCAAATCCGC GCTGCTCCCC CCACCGTCCC CTCGGGTCGG GAAGCGGTCC 
CCGCCGGGAC CCCCGGCCCA GCCCGCGGCC ACCCCCACGT CGCCCCACCG TCGCACTCAG 
GAGCCTGTGC TGCCCGAGGA CACCACCACC GAAGAGAAGC GAGGGAAGAA GTCCAAGTCG 
TCCGGGCCCT CCCTGGCGGG CACCGCGGAA TCCCGACCCC AGACGCCACT GAGCGAGGCC 
TCAGGCCGCC TGTCGGCGTT GGGCCGATCG CCTAGGCTGG TGCGCGCCGG CTCCCGCATC 
CTGGACAAGC TGCAGTTCTT CGAGGAGCGA CGGCGCAGCC TGGAGCGCAG CGACTCGCCG 
CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG CGCAAGGCCC GCTCTCTGGA GCAGCCCAAG 
TCGGAGCGCG GCGCACCGTG GGGCACCCCC GGGGCCTCGC AGGAAGAACT GCGGGCGCCA 
GGCAGCGTGG CCGAGCGGCG CCGCCTGTTC CAGCAGAAAG CGGCCTCGCT GGACGAGCGC 
ACGCGTCAGC GCAGCCCGGC CTCAGACCTC GAGCTGCGCT TCGCCCAGGA GCTGGGCCGC 
ATCCGCCGCT CCACGTCGCG GGAGGAGCTG GTGCGCTCGC ACGAGTCCCT GCGCGCCACG 
CTGCAGCGTG CCCCATCCCC TCGAGAGCCC GGCGAGCCCC CGCTCTTCTC TCGGCCCTCC 
ACCCCCAAGA CATCGCGGGC CGTGAGCCCC GCCGCCGCCC AGCCGCCCTC TCCGAGCAGC 
GCGGAGAAGC CGGGGGACGA GCCTGGGAGG CCCAGGAGCC GCGGGCCGGC GGGCAGGACA 
GAGCCGGGGG AAGGCCCGCA GCAGGAGGTT AGGCGTCGGG ACCAATTCCC GCTGACCCGG 
AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT GTGCCGCCCC CCGCCGCCGA TCCCCCAGAG 
GCCAGGACGA AAGCACCCCC CGGTCGGAAG CGGGAGCCCC CGGCGCAGGC CGTGCGCTTC 
CTGCCCTGGG CCACGCCGGG CCTGGAGGGC GCTGCTGTAC CCCAGACCTT GGAGAAGAAC 
AGGGCGGGGC CTGAGGCAGA GAAGAGGCTT CGCAGAGGGC CGGAGGAGGA CGGTCCCTGG 
GGGCCCTGGG ACCGCCGAGG GGCCCGCAGC CAGGGCAAAG GTCGCCGGGC CCGGCCCACC 
TCCCCTGAGC TCGAGTCTTC GGATGACTCC TACGTGTCCG CTGGAGAAGA GCCCCTAGAG 
GCCCCTGTGT TTGAGATCCC CCTGCAGAAT GTGGTGGTGG CACCAGGGGC AGATGTGCTG 
CTCAAGTGTA TCATCACTGC CAACCCCCCG CCCCAAGTGT CCTGGCACAA GGATGGGTCA 
GCGCTGCGCA GCGAGGGCCG CCTCCTCCTC CGGGCTGAGG GTGAGCGGCA CACCCTGCTG 
CTCAGGGAGG CCAGGGCAGC AGATGCCGGG AGCTATATGG CCACCGCCAC CAACGAGCTG 
GGCCAGGCCA CCTGTGCCGC CTCACTGACC GTGAGACCCG GTGGGTCTAC ATCCCCTTTC 
AGCAGCCCCA TCACCTCCGA CGAGGAATAC CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT 
GGGGAGACCT GGCCGCGAAC CCCCACCATG AAGCCCAGTC CCAGCCAGAA CCGCCGTTCT 
TCTGACACTG GCTCCAAGGC ACCCCCCACC TTCAAGGTCT CACTTATGGA CCAGTCAGTA 
AGAGAAGGCC AAGATGTCAT CATGAGCATC CGCGTGCAGG GGGAGCCCAA GCCTGTGGTC 
TCCTGGCTGA GAAACCGCCA GCCCGTGCGC CCAGACCAGC GGCGCTTTGC GGAGGAGGCT 
GAGGGTGGGC TGTGCCGGCT GCGGATCCTG GCTGCAGAGC GTGGCGATGC TGGTTTCTAC 
ACTTGCAAAG CGGTCAATGA GTATGGTGCT CGGCAGTGCG AGGCCCGCTT GGAGGTCCGA 
GCACACCCTG AAAGCCGGTC CCTGGCCGTG CTGGCCCCCC TGCAGGACGT GGACGTGGGG 
GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG GTGGCGGGGC CCACTGACGT GGAGGTGGAT 
TGGCTGTGCC GTGGCCGCCT GCTGCAGCCT GCACTGCTCA AATGCAAGAT GCATTTCGAT 
GGCCGCAAAT GCAAGCTGCT ACTTACATCT GTACATGAGG ACGACAGTGG CGTCTACACC 
TGCAAGCTCA GCACGGCCAA AGATGAGCTG ACCTGCAGTG CCCGGCTGAC CGTGCGGCCC 
TCGTTGGCAC CCCTGTTCAC ACGGCTGCTG GAAGATGTGG AGGTGTTGGA GGGCCGAGCT 
GCCCGTTTCG ACTGCAAGAT CAGTGGCACC CCGCCCCCTG TTGTTACCTG GACTCATTTT 
GGCTGCCCCA TGGAGGAGAG TGAGAACTTG CGGCTGCGGC AGGACGGGGG TCTGCACTCA 
CTGCACATTG CCCATGTGGG CAGCGAGGAC GAGGGGCTCT ATGCGGTCAG TGCTGTTAAC 
ACCCATGGCC AGGCCCACTG CTCAGCCCAG CTGTATGTAG AAGAGCCCCG GACAGCCGCC 
TCAGGCCCCA GCTCGAAGCT GGAGAAGATG CCATCCATTC CCGAGGAGCC AGAGCAGGGT 
GAGCTGGAGC GGCTGTCCAT TCCTGACTTC CTGCGGCCAC TGCAGGACCT GGAGGTGGGA 
CTGGCCAAGG AGGCCATGCT AGAGTGCCAG GTGACCGGCC TGCCCTACCC CACCATCAGC 
TGGTTCCACA ATGGCCACCG CATCCAGAGC AGCGACGACC GGCGCATGAC ACAGTACAGG 
GATGTCCATC GCTTGGTGTT CCCTGCCGTG GGGCCTCAGC ACGCCGGTGT CTACAAGAGC 
GTCATTGCCA ACAAGCTGGG CAAAGCTGCC TGCTATGCCC ACCTGTATGT CACAGATGTG 
GTCCCAGGCC CTCCAGATGG CGCCCCGCAG GTGGTGGCTG TGACGGGGAG GATGGTCACA 
CTCACATGGA ACCCCCCCAG GAGTCTGGAC ATGGCCATCG ACCCGGACTC CCTGACGTAC 
ACAGTGCAGC ACCAGGTGCT GGGCTCGGAC CAGTGGACGG CACTGGTCAC AGGCCTGCGG 
GAGCCAGGGT GGGCAGCCAC AGGGCTGCGT AAGGGGGTCC AGCACATCTT CCGGGTCCTC 
AGCACCACTG TCAAGAGCAG CAGCAAGCCC TCACCCCCTT CTGAGCCTGT GCAGCTGCTG 
GAGCACGGCC CAACCCTGGA GGAGGCCCCT GCCATGCTGG ACAAACCAGA CATCGTGTAT 
GTGGTGGAGG GACAGCCTGC CAGCGTCACC GTCACATTCA ACCATGTGGA GGCCCAGGTC 
GTCTGGAGGA GCTGCCGAGG GGCCCTCCTA GAGGCACGGG CCGGTGTGTA CGAGCTGAGC 
CAGCCAGATG ATGACCAGTA CTGTCTTCGG ATCTGCCGGG TGAGCCGCCG GGACATGGGG 
GCCCTCACCT GCACCGCCCG AAACCGTCAC GGCACACAGA CCTGCTCGGT CACATTGGAG 
CTGGCAGAGG CCCCTCGGTT TGAGTCCATC ATGGAGGACG TGGAGGTGGG GGCTGGGGAA 
ACTGCTCGCT TTGCGGTGGT GGTCGAGGGA AAACCACTGC CGGACATCAT GTGGTACAAG 
GACGAGGTGC TGCTGACCGA GAGCAGCCAT GTGAGCTTCG TGTACGAGGA GAATGAGTGC 
TCCCTGGTGG TGCTCAGCAC GGGGGCCCAG GATGGAGGCG TCTACACCTG CACCGCCCAG 
AACCTGGCGG GTGAGGTCTC CTGCAAAGCA GAGTTGGCTG TGCATTCAGC TCAGACAGCT 
ATGGAGGTCG AGGGGGTCGG GGAGGATGAG GACCATCGAG GAAGGAGACT CAGCGACTTT 
TATGACATCC ACCAGGAGAT CGGCAGGGGT GCTTTCTCCT ACTTGCGGCG CATAGTGGAG 
CGTAGCTCCG GCCTGGAGTT TGCGGCCAAG TTCATCCCCA GCCAGGCCAA GCCAAAGGCA 
TCAGCGCGTC GGGAGGCCCG GCTGCTGGCC AGGCTCCAGC ACGACTGTGT CCTCTACTTC 
CATGAGGCCT TCGAGAGGCG CCGGGGACTG GTCATTGTCA CCGAGCTCTG CACAGAGGAG 
CTGCTGGAGC GAATCGCCAG GAAACCCACC GTGTGTGAGT CTGAGATCCG GGCCTATATG 
CGGCAGGTGC TAGAGGGAAT ACACTACCTG CACCAGAGCC ACGTGCTGCA CCTCGATGTC 
AAGCCTGAGA ACCTGCTGGT GTGGGATGGT GCTGCGGGCG AGCAGCAGGT GCGGATCTGT 
GACTTTGGGA ATGCCCAGGA GCTGACTCCA GGAGAGCCCC AGTACTGCCA GTATGGCACA 
CCTGAGTTTG TAGCACCCGA GATTGTCAAT CAGAGCCCCG TGTCTGGAGT CACTGACATC 
TGGCCTGTGG GTGTTGTTGC CTTCCTCTGT CTGACAGGAA TCTCCCCGTT TGTTGGGGAA 
AATGACCGGA CAACATTGAT GAACATCCGA AACTACAACG TGGCCTTCGA GGAGACCACA 
TTCCTGAGCC TGAGCAGGGA GGCCCGGGGC TTCCTCATCA AAGTGTTGGT GCAGGACCGG 
CTGAGACCTA CCGCAGAAGA GACCCTAGAA CATCCTTGGT TCAAAACTCA GGCAAAGGGC 
GCAGAGGTGA GCACGGATCA CCTGAAGCTA TTCCTCTCCC GGCGGAGGTG GCAGCGCTCC 
CAGATCAGCT ACAAATGCCA CCTGGTGCTG CGCCCCATCC CCGAGCTGCT GCGGGCCCCC 
CCAGAGCGGG TGTGGGTGAC CATGCCCAGA AGGCCACCCC CCAGTGGGGG GCTCTCATCC 
TCCTCGGATT CTGAAGAGGA AGAGCTGGAA GAGCTGCCCT CAGTGCCCCG CCCACTGCAG 
CCCGAGTTCT CTGGCTCCCG GGTGTCCCTC ACAGACATTC CCACTGAGGA TGAGGCCCTG 
GGGACCCCAG AGACTGGGGC TGCCACCCCC ATGGACTGGC AGGAGCAGGG AAGGGCTCCC 
TCTCAGGACC AGGAGGCTCC CAGCCCAGAG GCCCTCCCCT CCCCAGGCCA GGAGCCCGCA 
GCTGGGGCTA GCCCCAGGCG GGGAGAGCTC CGCAGGGGCA GCTCGGCTGA GAGCGCCCTG 
CCCCGGGCCG GGCCGCGGGA GCTGGGCCGG GGCCTGCACA AGGCGGCGTC TGTGGAGCTG 
CCGCAGCGCC GGAGCCCCAG CCCGGGAGCC ACCCGCCTGG CCCGGGGAGG CCTGGGTGAG 
GGCGAGTATG CCCAGAGGCT GCAGGCCCTG CGCCAGCGGC TGCTGCGGGG AGGCCCCGAG 
GATGGCAAGG TCAGCGGCCT CAGGGGTCCC CTGCTGGAGA GCCTGGGGGG CCGTGCTCGG 
GACCCCCGGA TGGCACGAGC TGCCTCCAGC GAGGCAGCGC CCCACCACCA GCCCCCACTC 
GAGAACCGGG GCCTGCAAAA GAGCAGCAGC TTCTCCCAGG GTGAGGCGGA GCCCCGGGGC 
CGGCACCGCC GAGCGGGGGC GCCCCTCGAG ATCCCCGTGG CCAGGCTTGG GGCCCGTAGG 
CTACAGGAGT CTCCTTCCCT GTCTGCCCTC AGCGAGGCCC AGCCATCCAG CCCTGCACGG 
CCCAGCGCCC CCAAACCCAG TACCCCTAAG TCTGCAGAAC CTTCTGCCAC CACACCTAGT 
GATGCTCCGC AGCCCCCCGC ACCCCAGCCT GCCCAAGACA AGGCTCCAGA GCCCAGGCCA 
GAACCAGTCC GAGCCTCCAA GCCTGCACCA CCCCCCCAGG CCCTGCAAAC CCTAGCGCTG 
CCCCTCACAC CCTATGCTCA GATCATTCAG TCCCTCCAGC TGTCAGGCCA CGCCCAGGGC 
CCCTCGCAGG GCCCTGCCGC GCCGCCTTCA GAGCCCAAGC CCCACGCTGC TGTCTTTGCC 
AGGGTGGCCT CCCCACCTCC GGGAGCCCCC GAGAAGCGCG TGCCCTCAGC CGGGGGTCCC 
CCGGTGCTAG CCGAGAAAGC CCGAGTTCCC ACGGTGCCCC CCAGGCCAGG CAGCAGTCTC 
AGTAGCAGCA TCGAAAACTT GGAGTCGGAG GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC 
CGCGAGTCGC CCCTGTCGCT GGGGCTGCGG CTGCTGAGCC GTTCGCGCTC GGAGGAGCGC 
GGCCCCTTCC GTGGGGCCGA GGAGGAGGAT GGCATATACC GGCCCAGCCC GGCGGGGACC 
CCGCTGGAGC TGGTGCGACG GCCTGAGCGC TCACGCTCGG TGCAGGACCT CAGGGCTGTC 
GGAGAGCCTG GCCTCGTCCG CCGCCTCTCG CTGTCACTGT CCCAGCGGCT GCGGCGGACC 
CCTCCCGCGC AGCGCCACCC GGCCTGGGAG GCCCGCGGCG GGGACGGAGA GAGCTCGGAG 
GGCGGGAGCT CGGCGCGGGG CTCCCCGGTG CTGGCGATGC GCAGGCGGCT GAGCTTCACC 
CTGGAGCGGC TGTCCAGCCG ATTGCAGCGC AGTGGCAGCA GCGAGGACTC GGGGGGCGCG 
TCGGGCCGCA GCACGCCGCT GTTCGGACGG CTTCGCAGGG CCACGTCCGA GGGCGAGAGT 
CTGCGGCGCC TTGGCCTTCC GCACAACCAG TTGGCCGCCC AGGCCGGCGC CACCACGCCT 
TCCGCCGAGT CCCTGGGCTC CGAGGCCAGC GCCACGTCGG GCTCCTCAGC CCCAGGGGAA 
AGCCGAAGCC GGCTCCGCTG GGGCTTCTCT CGGCCGCGGA AGGACAAGGG GTTATCGCCA 
CCAAACCTCT CTGCCAGCGT CCAGGAGGAG TTGGGTCACC AGTACGTGCG CAGTGAGTCA 
GACTTCCCCC CAGTCTTCCA CATCAAACTC AAGGACCAGG TGCTGCTGGA GGGGGAGGCA 
GCCACCCTGC TCTGCCTGCC AGCGGCCTGC CCTGCACCGC ACATCTCCTG GATGAAAGAC 
AAGAAGTCCT TGAGGTCAGA GCCCTCAGTG ATCATCGTGT CCTGCAAAGA TGGGCGGCAG 
CTGCTCAGCA TCCCCCGGGC GGGCAAGCGG CACGCCGGTC TCTATGAGTG CTCGGCCACC 
AACGTACTGG GCAGCATCAC CAGCTCCTGT ACCGTGGCTG TGGCCCGAGT CCCAGGAAAG 
CTAGCTCCTC CAGAGGTACC CCAGACCTAC CAGGACACGG CGCTGGTGCT GTGGAAGCCG 
GGAGACAGCC GGGCACCTTG CACGTATACG CTGGAGCGGC GAGTGGATGG GGAGTCTGTG 
TGGCACCCTG TGAGCTCAGG CATCCCCGAC TGTTACTACA ACGTGACCCA CCTGCCAGTT 
GGCGTGACTG TGAGGTTCCG TGTGGCCTGT GCCAACCGTG CTGGGCAGGG GCCCTTCAGC 
AACTCTTCTG AGAAGGTCTT TGTCAGGGGT ACTCAAGATT CTTCAGCTGT GCCATCTGCT 
GCCCACCAAG AGGCCCCTGT CACCTCAAGG CCAGCCAGGG CCCGGCCTCC TGACTCTCCT 
ACCTCACTGG CCCCACCCCT AGCTCCTGCT GCCCCCACAC CCCCGTCAGT CACTGTCAGC 
CCCTCATCTC CCCCCACACC TCCTAGCCAG GCCTTGTCCT CGCTCAAGGC TGTGGGTCCA 
CCACCCCAAA CCCCTCCACG AAGACACAGG GGCCTGCAGG CTGCCCGGCC AGCGGAGCCC 
ACCCTACCCA GTACCCACGT CACCCCAAGT GAGCCCAAGC CTTTCGTCCT TGACACTGGG 
ACCCCGATCC CAGCCTCCAC TCCTCAAGGG GTTAAACCAG TGTCTTCCTC TACTCCTGTG 
TATGTGGTGA CTTCCTTTGT GTCTGCACCA CCAGCCCCTG AGCCCCCAGC CCCTGAGCCC 
CCTCCTGAGC CTACCAAGGT GACTGTGCAG AGCCTCAGCC CGGCCAAGGA GGTGGTCAGC 
TCCCCTGGGA GCAGTCCCCG AAGCTCTCCC AGGCCTGAGG GTACCACTCT TCGACAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAAGCCA GGGGCCGCTT TGGTGTTGTG 
CGAGCGTGCC GGGAGAATGC CACGGGGCGA ACGTTCGTGG CCAAGATCGT GCCCTATGCT 
GCCGAGGGCA AGCGGCGGGT CCTGCAGGAG TACGAGGTGC TGCGGACCCT GCACCACGAG 
CGGATCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT CATTGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGTGACA GGTTCCGGTA TTCTGAGGAT 
GACGTGGCCA CTTACATGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTCCACC TAGACATCAA GCCAGACAAC CTGCTGCTGG CCCCTGACAA TGCCCTCAAG 
ATTGTGGACT TTGGCAGTGC CCAGCCCTAC AACCCCCAGG CCCTTAGGCC CCTTGGCCAC 
CGCACGGGCA CGCTGGAGTT CATGGCTCCG GAGATGGTGA AGGGAGAACC CATCGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACTTACATTA TGCTCAGTGG ACGCTCCCCG 
TTCTATGAGC CAGACCCCCA GGAAACGGAG GCTCGGATTG TGGGGGGCCG CTTTGATGCC 
TTCCAGCTGT ACCCCAATAC ATCCCAGAGC GCCACCCTCT TCTTGCGAAA GGTTCTCTCT 
GTACATCCCT GGAGCCGGCC CTCCCTGCAG GACTGCCTGG CCCACCCATG GTTGCAGGAC 
GCCTACCTGA TGAAGCTGCG CCGCCAGACG CTCACCTTCA CCACCAACCG GCTCAAGGAG 
TTCCTGGGCG AGCAGCGGCG GCGCCGGGCT GAGGCTGCCA CCCGCCACAA GGTGCTGCTG 
CGCTCCTACC CTGGCGGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005867.3
CDS	1..9804
Translation	MQKARGTRGEDAGTRAPPSPGVPPKRAKVGAGGGAPVAVAGAPVFLRPLKNAAVCAGSDVRLRVVVSGTPQPSLRWFRDGQLLPAPAPEPSCLWLRRCGAQDAGVYSCMAQNERGRASCEAVLTVLEVGDSETAEDDISDVQGTQRLELRDDGAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRRLPGSPRQAQATGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSVPQSGLRREEPDLQPQLASEAPRRPAQPPPSKSALLPPPSPRVGKRSPPGPPAQPAATPTSPHRRTQEPVLPEDTTTEEKRGKKSKSSGPSLAGTAESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSERGAPWGTPGASQEELRAPGSVAERRRLFQQKAASLDERTRQRSPASDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAAAQPPSPSSAEKPGDEPGRPRSRGPAGRTEPGEGPQQEVRRRDQFPLTRSRAIQECRSPVPPPAADPPEARTKAPPGRKREPPAQAVRFLPWATPGLEGAAVPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGARSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNVVVAPGADVLLKCIITANPPPQVSWHKDGSALRSEGRLLLRAEGERHTLLLREARAADAGSYMATATNELGQATCAASLTVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPTDVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARFDCKISGTPPPVVTWTHFGCPMEESENLRLRQDGGLHSLHIAHVGSEDEGLYAVSAVNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEQGELERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDVHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPQVVAVTGRMVTLTWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPGWAATGLRKGVQHIFRVLSTTVKSSSKPSPPSEPVQLLEHGPTLEEAPAMLDKPDIVYVVEGQPASVTVTFNHVEAQVVWRSCRGALLEARAGVYELSQPDDDQYCLRICRVSRRDMGALTCTARNRHGTQTCSVTLELAEAPRFESIMEDVEVGAGETARFAVVVEGKPLPDIMWYKDEVLLTESSHVSFVYEENECSLVVLSTGAQDGGVYTCTAQNLAGEVSCKAELAVHSAQTAMEVEGVGEDEDHRGRRLSDFYDIHQEIGRGAFSYLRRIVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHDCVLYFHEAFERRRGLVIVTELCTEELLERIARKPTVCESEIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTQAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGELRRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQPPAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPSEPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEAKFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAVGEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAESLGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPHISWMKDKKSLRSEPSVIIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARVPGKLAPPEVPQTYQDTALVLWKPGDSRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTHLPVGVTVRFRVACANRAGQGPFSNSSEKVFVRGTQDSSAVPSAAHQEAPVTSRPARARPPDSPTSLAPPLAPAAPTPPSVTVSPSSPPTPPSQALSSLKAVGPPPQTPPRRHRGLQAARPAEPTLPSTHVTPSEPKPFVLDTGTPIPASTPQGVKPVSSSTPVYVVTSFVSAPPAPEPPAPEPPPEPTKVTVQSLSPAKEVVSSPGSSPRSSPRPEGTTLRQGPPQKPYTFLEEKARGRFGVVRACRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERIMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYMVQLLQGLDYLHGHHVLHLDIKPDNLLLAPDNALKIVDFGSAQPYNPQALRPLGHRTGTLEFMAPEMVKGEPIGSATDIWGAGVLTYIMLSGRSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGGP

Target ORF information:

RefSeq Version	NM_005876.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SPEG complex locus (SPEG), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005876.4

ORF Insert Sequence:

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GATGCGGGCA CGAGGGCACC CCCCAGCCCC 
GGAGTGCCCC CGAAAAGGGC CAAGGTGGGG GCCGGCGGCG GGGCTCCTGT GGCCGTGGCC 
GGGGCGCCAG TCTTCCTGCG GCCCCTGAAG AACGCGGCGG TGTGCGCGGG CAGCGACGTG 
CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCCGCTGGTT CCGGGATGGG 
CAGCTCCTGC CCGCGCCGGC CCCCGAGCCC AGCTGCCTGT GGCTGCGGCG CTGCGGGGCG 
CAGGACGCCG GCGTGTACAG CTGCATGGCC CAGAACGAGC GGGGCCGGGC CTCCTGCGAG 
GCGGTGCTCA CAGTGCTGGA GGTCGGAGAC TCAGAGACGG CTGAGGATGA CATCAGCGAT 
GTGCAGGGAA CCCAGCGCCT GGAGCTTCGG GATGACGGGG CCTTCAGCAC CCCCACGGGG 
GGTTCTGACA CCCTGGTGGG CACCTCCCTG GACACACCCC CGACCTCCGT GACAGGCACC 
TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC 
AGTGGGGGTG GCACCCGCCG CCTCCCGGGC AGCCCAAGGC AAGCACAGGC AACCGGGGCC 
GGGCCACGGC ACCTGGGGGT GGAGCCGCTG GTGCGGGCAT CTCGAGCTAA TCTGGTGGGC 
GCAAGCTGGG GGTCAGAGGA TAGCCTTTCC GTGGCCAGTG ACCTGTACGG CAGCGCATTC 
AGCCTGTACA GAGGACGGGC GCTCTCTATC CACGTCAGCG TCCCTCAGAG CGGGTTGCGC 
AGGGAGGAGC CCGACCTTCA GCCTCAACTG GCCAGCGAAG CCCCACGCCG CCCTGCCCAG 
CCGCCTCCTT CCAAATCCGC GCTGCTCCCC CCACCGTCCC CTCGGGTCGG GAAGCGGTCC 
CCGCCGGGAC CCCCGGCCCA GCCCGCGGCC ACCCCCACGT CGCCCCACCG TCGCACTCAG 
GAGCCTGTGC TGCCCGAGGA CACCACCACC GAAGAGAAGC GAGGGAAGAA GTCCAAGTCG 
TCCGGGCCCT CCCTGGCGGG CACCGCGGAA TCCCGACCCC AGACGCCACT GAGCGAGGCC 
TCAGGCCGCC TGTCGGCGTT GGGCCGATCG CCTAGGCTGG TGCGCGCCGG CTCCCGCATC 
CTGGACAAGC TGCAGTTCTT CGAGGAGCGA CGGCGCAGCC TGGAGCGCAG CGACTCGCCG 
CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG CGCAAGGCCC GCTCTCTGGA GCAGCCCAAG 
TCGGAGCGCG GCGCACCGTG GGGCACCCCC GGGGCCTCGC AGGAAGAACT GCGGGCGCCA 
GGCAGCGTGG CCGAGCGGCG CCGCCTGTTC CAGCAGAAAG CGGCCTCGCT GGACGAGCGC 
ACGCGTCAGC GCAGCCCGGC CTCAGACCTC GAGCTGCGCT TCGCCCAGGA GCTGGGCCGC 
ATCCGCCGCT CCACGTCGCG GGAGGAGCTG GTGCGCTCGC ACGAGTCCCT GCGCGCCACG 
CTGCAGCGTG CCCCATCCCC TCGAGAGCCC GGCGAGCCCC CGCTCTTCTC TCGGCCCTCC 
ACCCCCAAGA CATCGCGGGC CGTGAGCCCC GCCGCCGCCC AGCCGCCCTC TCCGAGCAGC 
GCGGAGAAGC CGGGGGACGA GCCTGGGAGG CCCAGGAGCC GCGGGCCGGC GGGCAGGACA 
GAGCCGGGGG AAGGCCCGCA GCAGGAGGTT AGGCGTCGGG ACCAATTCCC GCTGACCCGG 
AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT GTGCCGCCCC CCGCCGCCGA TCCCCCAGAG 
GCCAGGACGA AAGCACCCCC CGGTCGGAAG CGGGAGCCCC CGGCGCAGGC CGTGCGCTTC 
CTGCCCTGGG CCACGCCGGG CCTGGAGGGC GCTGCTGTAC CCCAGACCTT GGAGAAGAAC 
AGGGCGGGGC CTGAGGCAGA GAAGAGGCTT CGCAGAGGGC CGGAGGAGGA CGGTCCCTGG 
GGGCCCTGGG ACCGCCGAGG GGCCCGCAGC CAGGGCAAAG GTCGCCGGGC CCGGCCCACC 
TCCCCTGAGC TCGAGTCTTC GGATGACTCC TACGTGTCCG CTGGAGAAGA GCCCCTAGAG 
GCCCCTGTGT TTGAGATCCC CCTGCAGAAT GTGGTGGTGG CACCAGGGGC AGATGTGCTG 
CTCAAGTGTA TCATCACTGC CAACCCCCCG CCCCAAGTGT CCTGGCACAA GGATGGGTCA 
GCGCTGCGCA GCGAGGGCCG CCTCCTCCTC CGGGCTGAGG GTGAGCGGCA CACCCTGCTG 
CTCAGGGAGG CCAGGGCAGC AGATGCCGGG AGCTATATGG CCACCGCCAC CAACGAGCTG 
GGCCAGGCCA CCTGTGCCGC CTCACTGACC GTGAGACCCG GTGGGTCTAC ATCCCCTTTC 
AGCAGCCCCA TCACCTCCGA CGAGGAATAC CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT 
GGGGAGACCT GGCCGCGAAC CCCCACCATG AAGCCCAGTC CCAGCCAGAA CCGCCGTTCT 
TCTGACACTG GCTCCAAGGC ACCCCCCACC TTCAAGGTCT CACTTATGGA CCAGTCAGTA 
AGAGAAGGCC AAGATGTCAT CATGAGCATC CGCGTGCAGG GGGAGCCCAA GCCTGTGGTC 
TCCTGGCTGA GAAACCGCCA GCCCGTGCGC CCAGACCAGC GGCGCTTTGC GGAGGAGGCT 
GAGGGTGGGC TGTGCCGGCT GCGGATCCTG GCTGCAGAGC GTGGCGATGC TGGTTTCTAC 
ACTTGCAAAG CGGTCAATGA GTATGGTGCT CGGCAGTGCG AGGCCCGCTT GGAGGTCCGA 
GCACACCCTG AAAGCCGGTC CCTGGCCGTG CTGGCCCCCC TGCAGGACGT GGACGTGGGG 
GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG GTGGCGGGGC CCACTGACGT GGAGGTGGAT 
TGGCTGTGCC GTGGCCGCCT GCTGCAGCCT GCACTGCTCA AATGCAAGAT GCATTTCGAT 
GGCCGCAAAT GCAAGCTGCT ACTTACATCT GTACATGAGG ACGACAGTGG CGTCTACACC 
TGCAAGCTCA GCACGGCCAA AGATGAGCTG ACCTGCAGTG CCCGGCTGAC CGTGCGGCCC 
TCGTTGGCAC CCCTGTTCAC ACGGCTGCTG GAAGATGTGG AGGTGTTGGA GGGCCGAGCT 
GCCCGTTTCG ACTGCAAGAT CAGTGGCACC CCGCCCCCTG TTGTTACCTG GACTCATTTT 
GGCTGCCCCA TGGAGGAGAG TGAGAACTTG CGGCTGCGGC AGGACGGGGG TCTGCACTCA 
CTGCACATTG CCCATGTGGG CAGCGAGGAC GAGGGGCTCT ATGCGGTCAG TGCTGTTAAC 
ACCCATGGCC AGGCCCACTG CTCAGCCCAG CTGTATGTAG AAGAGCCCCG GACAGCCGCC 
TCAGGCCCCA GCTCGAAGCT GGAGAAGATG CCATCCATTC CCGAGGAGCC AGAGCAGGGT 
GAGCTGGAGC GGCTGTCCAT TCCTGACTTC CTGCGGCCAC TGCAGGACCT GGAGGTGGGA 
CTGGCCAAGG AGGCCATGCT AGAGTGCCAG GTGACCGGCC TGCCCTACCC CACCATCAGC 
TGGTTCCACA ATGGCCACCG CATCCAGAGC AGCGACGACC GGCGCATGAC ACAGTACAGG 
GATGTCCATC GCTTGGTGTT CCCTGCCGTG GGGCCTCAGC ACGCCGGTGT CTACAAGAGC 
GTCATTGCCA ACAAGCTGGG CAAAGCTGCC TGCTATGCCC ACCTGTATGT CACAGATGTG 
GTCCCAGGCC CTCCAGATGG CGCCCCGCAG GTGGTGGCTG TGACGGGGAG GATGGTCACA 
CTCACATGGA ACCCCCCCAG GAGTCTGGAC ATGGCCATCG ACCCGGACTC CCTGACGTAC 
ACAGTGCAGC ACCAGGTGCT GGGCTCGGAC CAGTGGACGG CACTGGTCAC AGGCCTGCGG 
GAGCCAGGGT GGGCAGCCAC AGGGCTGCGT AAGGGGGTCC AGCACATCTT CCGGGTCCTC 
AGCACCACTG TCAAGAGCAG CAGCAAGCCC TCACCCCCTT CTGAGCCTGT GCAGCTGCTG 
GAGCACGGCC CAACCCTGGA GGAGGCCCCT GCCATGCTGG ACAAACCAGA CATCGTGTAT 
GTGGTGGAGG GACAGCCTGC CAGCGTCACC GTCACATTCA ACCATGTGGA GGCCCAGGTC 
GTCTGGAGGA GCTGCCGAGG GGCCCTCCTA GAGGCACGGG CCGGTGTGTA CGAGCTGAGC 
CAGCCAGATG ATGACCAGTA CTGTCTTCGG ATCTGCCGGG TGAGCCGCCG GGACATGGGG 
GCCCTCACCT GCACCGCCCG AAACCGTCAC GGCACACAGA CCTGCTCGGT CACATTGGAG 
CTGGCAGAGG CCCCTCGGTT TGAGTCCATC ATGGAGGACG TGGAGGTGGG GGCTGGGGAA 
ACTGCTCGCT TTGCGGTGGT GGTCGAGGGA AAACCACTGC CGGACATCAT GTGGTACAAG 
GACGAGGTGC TGCTGACCGA GAGCAGCCAT GTGAGCTTCG TGTACGAGGA GAATGAGTGC 
TCCCTGGTGG TGCTCAGCAC GGGGGCCCAG GATGGAGGCG TCTACACCTG CACCGCCCAG 
AACCTGGCGG GTGAGGTCTC CTGCAAAGCA GAGTTGGCTG TGCATTCAGC TCAGACAGCT 
ATGGAGGTCG AGGGGGTCGG GGAGGATGAG GACCATCGAG GAAGGAGACT CAGCGACTTT 
TATGACATCC ACCAGGAGAT CGGCAGGGGT GCTTTCTCCT ACTTGCGGCG CATAGTGGAG 
CGTAGCTCCG GCCTGGAGTT TGCGGCCAAG TTCATCCCCA GCCAGGCCAA GCCAAAGGCA 
TCAGCGCGTC GGGAGGCCCG GCTGCTGGCC AGGCTCCAGC ACGACTGTGT CCTCTACTTC 
CATGAGGCCT TCGAGAGGCG CCGGGGACTG GTCATTGTCA CCGAGCTCTG CACAGAGGAG 
CTGCTGGAGC GAATCGCCAG GAAACCCACC GTGTGTGAGT CTGAGATCCG GGCCTATATG 
CGGCAGGTGC TAGAGGGAAT ACACTACCTG CACCAGAGCC ACGTGCTGCA CCTCGATGTC 
AAGCCTGAGA ACCTGCTGGT GTGGGATGGT GCTGCGGGCG AGCAGCAGGT GCGGATCTGT 
GACTTTGGGA ATGCCCAGGA GCTGACTCCA GGAGAGCCCC AGTACTGCCA GTATGGCACA 
CCTGAGTTTG TAGCACCCGA GATTGTCAAT CAGAGCCCCG TGTCTGGAGT CACTGACATC 
TGGCCTGTGG GTGTTGTTGC CTTCCTCTGT CTGACAGGAA TCTCCCCGTT TGTTGGGGAA 
AATGACCGGA CAACATTGAT GAACATCCGA AACTACAACG TGGCCTTCGA GGAGACCACA 
TTCCTGAGCC TGAGCAGGGA GGCCCGGGGC TTCCTCATCA AAGTGTTGGT GCAGGACCGG 
CTGAGACCTA CCGCAGAAGA GACCCTAGAA CATCCTTGGT TCAAAACTCA GGCAAAGGGC 
GCAGAGGTGA GCACGGATCA CCTGAAGCTA TTCCTCTCCC GGCGGAGGTG GCAGCGCTCC 
CAGATCAGCT ACAAATGCCA CCTGGTGCTG CGCCCCATCC CCGAGCTGCT GCGGGCCCCC 
CCAGAGCGGG TGTGGGTGAC CATGCCCAGA AGGCCACCCC CCAGTGGGGG GCTCTCATCC 
TCCTCGGATT CTGAAGAGGA AGAGCTGGAA GAGCTGCCCT CAGTGCCCCG CCCACTGCAG 
CCCGAGTTCT CTGGCTCCCG GGTGTCCCTC ACAGACATTC CCACTGAGGA TGAGGCCCTG 
GGGACCCCAG AGACTGGGGC TGCCACCCCC ATGGACTGGC AGGAGCAGGG AAGGGCTCCC 
TCTCAGGACC AGGAGGCTCC CAGCCCAGAG GCCCTCCCCT CCCCAGGCCA GGAGCCCGCA 
GCTGGGGCTA GCCCCAGGCG GGGAGAGCTC CGCAGGGGCA GCTCGGCTGA GAGCGCCCTG 
CCCCGGGCCG GGCCGCGGGA GCTGGGCCGG GGCCTGCACA AGGCGGCGTC TGTGGAGCTG 
CCGCAGCGCC GGAGCCCCAG CCCGGGAGCC ACCCGCCTGG CCCGGGGAGG CCTGGGTGAG 
GGCGAGTATG CCCAGAGGCT GCAGGCCCTG CGCCAGCGGC TGCTGCGGGG AGGCCCCGAG 
GATGGCAAGG TCAGCGGCCT CAGGGGTCCC CTGCTGGAGA GCCTGGGGGG CCGTGCTCGG 
GACCCCCGGA TGGCACGAGC TGCCTCCAGC GAGGCAGCGC CCCACCACCA GCCCCCACTC 
GAGAACCGGG GCCTGCAAAA GAGCAGCAGC TTCTCCCAGG GTGAGGCGGA GCCCCGGGGC 
CGGCACCGCC GAGCGGGGGC GCCCCTCGAG ATCCCCGTGG CCAGGCTTGG GGCCCGTAGG 
CTACAGGAGT CTCCTTCCCT GTCTGCCCTC AGCGAGGCCC AGCCATCCAG CCCTGCACGG 
CCCAGCGCCC CCAAACCCAG TACCCCTAAG TCTGCAGAAC CTTCTGCCAC CACACCTAGT 
GATGCTCCGC AGCCCCCCGC ACCCCAGCCT GCCCAAGACA AGGCTCCAGA GCCCAGGCCA 
GAACCAGTCC GAGCCTCCAA GCCTGCACCA CCCCCCCAGG CCCTGCAAAC CCTAGCGCTG 
CCCCTCACAC CCTATGCTCA GATCATTCAG TCCCTCCAGC TGTCAGGCCA CGCCCAGGGC 
CCCTCGCAGG GCCCTGCCGC GCCGCCTTCA GAGCCCAAGC CCCACGCTGC TGTCTTTGCC 
AGGGTGGCCT CCCCACCTCC GGGAGCCCCC GAGAAGCGCG TGCCCTCAGC CGGGGGTCCC 
CCGGTGCTAG CCGAGAAAGC CCGAGTTCCC ACGGTGCCCC CCAGGCCAGG CAGCAGTCTC 
AGTAGCAGCA TCGAAAACTT GGAGTCGGAG GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC 
CGCGAGTCGC CCCTGTCGCT GGGGCTGCGG CTGCTGAGCC GTTCGCGCTC GGAGGAGCGC 
GGCCCCTTCC GTGGGGCCGA GGAGGAGGAT GGCATATACC GGCCCAGCCC GGCGGGGACC 
CCGCTGGAGC TGGTGCGACG GCCTGAGCGC TCACGCTCGG TGCAGGACCT CAGGGCTGTC 
GGAGAGCCTG GCCTCGTCCG CCGCCTCTCG CTGTCACTGT CCCAGCGGCT GCGGCGGACC 
CCTCCCGCGC AGCGCCACCC GGCCTGGGAG GCCCGCGGCG GGGACGGAGA GAGCTCGGAG 
GGCGGGAGCT CGGCGCGGGG CTCCCCGGTG CTGGCGATGC GCAGGCGGCT GAGCTTCACC 
CTGGAGCGGC TGTCCAGCCG ATTGCAGCGC AGTGGCAGCA GCGAGGACTC GGGGGGCGCG 
TCGGGCCGCA GCACGCCGCT GTTCGGACGG CTTCGCAGGG CCACGTCCGA GGGCGAGAGT 
CTGCGGCGCC TTGGCCTTCC GCACAACCAG TTGGCCGCCC AGGCCGGCGC CACCACGCCT 
TCCGCCGAGT CCCTGGGCTC CGAGGCCAGC GCCACGTCGG GCTCCTCAGC CCCAGGGGAA 
AGCCGAAGCC GGCTCCGCTG GGGCTTCTCT CGGCCGCGGA AGGACAAGGG GTTATCGCCA 
CCAAACCTCT CTGCCAGCGT CCAGGAGGAG TTGGGTCACC AGTACGTGCG CAGTGAGTCA 
GACTTCCCCC CAGTCTTCCA CATCAAACTC AAGGACCAGG TGCTGCTGGA GGGGGAGGCA 
GCCACCCTGC TCTGCCTGCC AGCGGCCTGC CCTGCACCGC ACATCTCCTG GATGAAAGAC 
AAGAAGTCCT TGAGGTCAGA GCCCTCAGTG ATCATCGTGT CCTGCAAAGA TGGGCGGCAG 
CTGCTCAGCA TCCCCCGGGC GGGCAAGCGG CACGCCGGTC TCTATGAGTG CTCGGCCACC 
AACGTACTGG GCAGCATCAC CAGCTCCTGT ACCGTGGCTG TGGCCCGAGT CCCAGGAAAG 
CTAGCTCCTC CAGAGGTACC CCAGACCTAC CAGGACACGG CGCTGGTGCT GTGGAAGCCG 
GGAGACAGCC GGGCACCTTG CACGTATACG CTGGAGCGGC GAGTGGATGG GGAGTCTGTG 
TGGCACCCTG TGAGCTCAGG CATCCCCGAC TGTTACTACA ACGTGACCCA CCTGCCAGTT 
GGCGTGACTG TGAGGTTCCG TGTGGCCTGT GCCAACCGTG CTGGGCAGGG GCCCTTCAGC 
AACTCTTCTG AGAAGGTCTT TGTCAGGGGT ACTCAAGATT CTTCAGCTGT GCCATCTGCT 
GCCCACCAAG AGGCCCCTGT CACCTCAAGG CCAGCCAGGG CCCGGCCTCC TGACTCTCCT 
ACCTCACTGG CCCCACCCCT AGCTCCTGCT GCCCCCACAC CCCCGTCAGT CACTGTCAGC 
CCCTCATCTC CCCCCACACC TCCTAGCCAG GCCTTGTCCT CGCTCAAGGC TGTGGGTCCA 
CCACCCCAAA CCCCTCCACG AAGACACAGG GGCCTGCAGG CTGCCCGGCC AGCGGAGCCC 
ACCCTACCCA GTACCCACGT CACCCCAAGT GAGCCCAAGC CTTTCGTCCT TGACACTGGG 
ACCCCGATCC CAGCCTCCAC TCCTCAAGGG GTTAAACCAG TGTCTTCCTC TACTCCTGTG 
TATGTGGTGA CTTCCTTTGT GTCTGCACCA CCAGCCCCTG AGCCCCCAGC CCCTGAGCCC 
CCTCCTGAGC CTACCAAGGT GACTGTGCAG AGCCTCAGCC CGGCCAAGGA GGTGGTCAGC 
TCCCCTGGGA GCAGTCCCCG AAGCTCTCCC AGGCCTGAGG GTACCACTCT TCGACAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAAGCCA GGGGCCGCTT TGGTGTTGTG 
CGAGCGTGCC GGGAGAATGC CACGGGGCGA ACGTTCGTGG CCAAGATCGT GCCCTATGCT 
GCCGAGGGCA AGCGGCGGGT CCTGCAGGAG TACGAGGTGC TGCGGACCCT GCACCACGAG 
CGGATCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT CATTGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGTGACA GGTTCCGGTA TTCTGAGGAT 
GACGTGGCCA CTTACATGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTCCACC TAGACATCAA GCCAGACAAC CTGCTGCTGG CCCCTGACAA TGCCCTCAAG 
ATTGTGGACT TTGGCAGTGC CCAGCCCTAC AACCCCCAGG CCCTTAGGCC CCTTGGCCAC 
CGCACGGGCA CGCTGGAGTT CATGGCTCCG GAGATGGTGA AGGGAGAACC CATCGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACTTACATTA TGCTCAGTGG ACGCTCCCCG 
TTCTATGAGC CAGACCCCCA GGAAACGGAG GCTCGGATTG TGGGGGGCCG CTTTGATGCC 
TTCCAGCTGT ACCCCAATAC ATCCCAGAGC GCCACCCTCT TCTTGCGAAA GGTTCTCTCT 
GTACATCCCT GGAGCCGGCC CTCCCTGCAG GACTGCCTGG CCCACCCATG GTTGCAGGAC 
GCCTACCTGA TGAAGCTGCG CCGCCAGACG CTCACCTTCA CCACCAACCG GCTCAAGGAG 
TTCCTGGGCG AGCAGCGGCG GCGCCGGGCT GAGGCTGCCA CCCGCCACAA GGTGCTGCTG 
CGCTCCTACC CTGGCGGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_005246237.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005246294.1 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X5, mRNA.

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CloneID	OHu35751
Clone ID Related Accession (Same CDS sequence)	XM_005246237.2
Accession Version	XM_005246237.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005246237.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTCA CCCATGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC AGAGACGGCT 
GAGGATGACA TCAGCGATGT GCAGGGAACC CAGCGCCTGG AGCTTCGGGA TGACGGGGCC 
TTCAGCACCC CCACGGGGGG TTCTGACACC CTGGTGGGCA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG TGGGGGTGGC ACCCGCCGCC TCCCGGGCAG CCCAAGGCAA 
GCACAGGCAA CCGGGGCCGG GCCACGGCAC CTGGGGGTGG AGCCGCTGGT GCGGGCATCT 
CGAGCTAATC TGGTGGGCGC AAGCTGGGGG TCAGAGGATA GCCTTTCCGT GGCCAGTGAC 
CTGTACGGCA GCGCATTCAG CCTGTACAGA GGACGGGCGC TCTCTATCCA CGTCAGCGTC 
CCTCAGAGCG GGTTGCGCAG GGAGGAGCCC GACCTTCAGC CTCAACTGGC CAGCGAAGCC 
CCACGCCGCC CTGCCCAGCC GCCTCCTTCC AAATCCGCGC TGCTCCCCCC ACCGTCCCCT 
CGGGTCGGGA AGCGGTCCCC GCCGGGACCC CCGGCCCAGC CCGCGGCCAC CCCCACGTCG 
CCCCACCGTC GCACTCAGGA GCCTGTGCTG CCCGAGGACA CCACCACCGA AGAGAAGCGA 
GGGAAGAAGT CCAAGTCGTC CGGGCCCTCC CTGGCGGGCA CCGCGGAATC CCGACCCCAG 
ACGCCACTGA GCGAGGCCTC AGGCCGCCTG TCGGCGTTGG GCCGATCGCC TAGGCTGGTG 
CGCGCCGGCT CCCGCATCCT GGACAAGCTG CAGTTCTTCG AGGAGCGACG GCGCAGCCTG 
GAGCGCAGCG ACTCGCCGCC GGCGCCCCTG CGGCCCTGGG TGCCCCTGCG CAAGGCCCGC 
TCTCTGGAGC AGCCCAAGTC GGAGCGCGGC GCACCGTGGG GCACCCCCGG GGCCTCGCAG 
GAAGAACTGC GGGCGCCAGG CAGCGTGGCC GAGCGGCGCC GCCTGTTCCA GCAGAAAGCG 
GCCTCGCTGG ACGAGCGCAC GCGTCAGCGC AGCCCGGCCT CAGACCTCGA GCTGCGCTTC 
GCCCAGGAGC TGGGCCGCAT CCGCCGCTCC ACGTCGCGGG AGGAGCTGGT GCGCTCGCAC 
GAGTCCCTGC GCGCCACGCT GCAGCGTGCC CCATCCCCTC GAGAGCCCGG CGAGCCCCCG 
CTCTTCTCTC GGCCCTCCAC CCCCAAGACA TCGCGGGCCG TGAGCCCCGC CGCCGCCCAG 
CCGCCCTCTC CGAGCAGCGC GGAGAAGCCG GGGGACGAGC CTGGGAGGCC CAGGAGCCGC 
GGGCCGGCGG GCAGGACAGA GCCGGGGGAA GGCCCGCAGC AGGAGGTTAG GCGTCGGGAC 
CAATTCCCGC TGACCCGGAG CAGAGCCATC CAGGAGTGCA GGAGCCCTGT GCCGCCCCCC 
GCCGCCGATC CCCCAGAGGC CAGGACGAAA GCACCCCCCG GTCGGAAGCG GGAGCCCCCG 
GCGCAGGCCG TGCGCTTCCT GCCCTGGGCC ACGCCGGGCC TGGAGGGCGC TGCTGTACCC 
CAGACCTTGG AGAAGAACAG GGCGGGGCCT GAGGCAGAGA AGAGGCTTCG CAGAGGGCCG 
GAGGAGGACG GTCCCTGGGG GCCCTGGGAC CGCCGAGGGG CCCGCAGCCA GGGCAAAGGT 
CGCCGGGCCC GGCCCACCTC CCCTGAGCTC GAGTCTTCGG ATGACTCCTA CGTGTCCGCT 
GGAGAAGAGC CCCTAGAGGC CCCTGTGTTT GAGATCCCCC TGCAGAATGT GGTGGTGGCA 
CCAGGGGCAG ATGTGCTGCT CAAGTGTATC ATCACTGCCA ACCCCCCGCC CCAAGTGTCC 
TGGCACAAGG ATGGGTCAGC GCTGCGCAGC GAGGGCCGCC TCCTCCTCCG GGCTGAGGGT 
GAGCGGCACA CCCTGCTGCT CAGGGAGGCC AGGGCAGCAG ATGCCGGGAG CTATATGGCC 
ACCGCCACCA ACGAGCTGGG CCAGGCCACC TGTGCCGCCT CACTGACCGT GAGACCCGGT 
GGGTCTACAT CCCCTTTCAG CAGCCCCATC ACCTCCGACG AGGAATACCT GAGCCCCCCA 
GAGGAGTTCC CAGAGCCTGG GGAGACCTGG CCGCGAACCC CCACCATGAA GCCCAGTCCC 
AGCCAGAACC GCCGTTCTTC TGACACTGGC TCCAAGGCAC CCCCCACCTT CAAGGTCTCA 
CTTATGGACC AGTCAGTAAG AGAAGGCCAA GATGTCATCA TGAGCATCCG CGTGCAGGGG 
GAGCCCAAGC CTGTGGTCTC CTGGCTGAGA AACCGCCAGC CCGTGCGCCC AGACCAGCGG 
CGCTTTGCGG AGGAGGCTGA GGGTGGGCTG TGCCGGCTGC GGATCCTGGC TGCAGAGCGT 
GGCGATGCTG GTTTCTACAC TTGCAAAGCG GTCAATGAGT ATGGTGCTCG GCAGTGCGAG 
GCCCGCTTGG AGGTCCGAGC ACACCCTGAA AGCCGGTCCC TGGCCGTGCT GGCCCCCCTG 
CAGGACGTGG ACGTGGGGGC CGGGGAGATG GCGCTGTTTG AGTGCCTGGT GGCGGGGCCC 
ACTGACGTGG AGGTGGATTG GCTGTGCCGT GGCCGCCTGC TGCAGCCTGC ACTGCTCAAA 
TGCAAGATGC ATTTCGATGG CCGCAAATGC AAGCTGCTAC TTACATCTGT ACATGAGGAC 
GACAGTGGCG TCTACACCTG CAAGCTCAGC ACGGCCAAAG ATGAGCTGAC CTGCAGTGCC 
CGGCTGACCG TGCGGCCCTC GTTGGCACCC CTGTTCACAC GGCTGCTGGA AGATGTGGAG 
GTGTTGGAGG GCCGAGCTGC CCGTTTCGAC TGCAAGATCA GTGGCACCCC GCCCCCTGTT 
GTTACCTGGA CTCATTTTGG CTGCCCCATG GAGGAGAGTG AGAACTTGCG GCTGCGGCAG 
GACGGGGGTC TGCACTCACT GCACATTGCC CATGTGGGCA GCGAGGACGA GGGGCTCTAT 
GCGGTCAGTG CTGTTAACAC CCATGGCCAG GCCCACTGCT CAGCCCAGCT GTATGTAGAA 
GAGCCCCGGA CAGCCGCCTC AGGCCCCAGC TCGAAGCTGG AGAAGATGCC ATCCATTCCC 
GAGGAGCCAG AGCAGGGTGA GCTGGAGCGG CTGTCCATTC CTGACTTCCT GCGGCCACTG 
CAGGACCTGG AGGTGGGACT GGCCAAGGAG GCCATGCTAG AGTGCCAGGT GACCGGCCTG 
CCCTACCCCA CCATCAGCTG GTTCCACAAT GGCCACCGCA TCCAGAGCAG CGACGACCGG 
CGCATGACAC AGTACAGGGA TGTCCATCGC TTGGTGTTCC CTGCCGTGGG GCCTCAGCAC 
GCCGGTGTCT ACAAGAGCGT CATTGCCAAC AAGCTGGGCA AAGCTGCCTG CTATGCCCAC 
CTGTATGTCA CAGATGTGGT CCCAGGCCCT CCAGATGGCG CCCCGCAGGT GGTGGCTGTG 
ACGGGGAGGA TGGTCACACT CACATGGAAC CCCCCCAGGA GTCTGGACAT GGCCATCGAC 
CCGGACTCCC TGACGTACAC AGTGCAGCAC CAGGTGCTGG GCTCGGACCA GTGGACGGCA 
CTGGTCACAG GCCTGCGGGA GCCAGGGTGG GCAGCCACAG GGCTGCGTAA GGGGGTCCAG 
CACATCTTCC GGGTCCTCAG CACCACTGTC AAGAGCAGCA GCAAGCCCTC ACCCCCTTCT 
GAGCCTGTGC AGCTGCTGGA GCACGGCCCA ACCCTGGAGG AGGCCCCTGC CATGCTGGAC 
AAACCAGACA TCGTGTATGT GGTGGAGGGA CAGCCTGCCA GCGTCACCGT CACATTCAAC 
CATGTGGAGG CCCAGGTCGT CTGGAGGAGC TGCCGAGGGG CCCTCCTAGA GGCACGGGCC 
GGTGTGTACG AGCTGAGCCA GCCAGATGAT GACCAGTACT GTCTTCGGAT CTGCCGGGTG 
AGCCGCCGGG ACATGGGGGC CCTCACCTGC ACCGCCCGAA ACCGTCACGG CACACAGACC 
TGCTCGGTCA CATTGGAGCT GGCAGAGGCC CCTCGGTTTG AGTCCATCAT GGAGGACGTG 
GAGGTGGGGG CTGGGGAAAC TGCTCGCTTT GCGGTGGTGG TCGAGGGAAA ACCACTGCCG 
GACATCATGT GGTACAAGGA CGAGGTGCTG CTGACCGAGA GCAGCCATGT GAGCTTCGTG 
TACGAGGAGA ATGAGTGCTC CCTGGTGGTG CTCAGCACGG GGGCCCAGGA TGGAGGCGTC 
TACACCTGCA CCGCCCAGAA CCTGGCGGGT GAGGTCTCCT GCAAAGCAGA GTTGGCTGTG 
CATTCAGCTC AGACAGCTAT GGAGGTCGAG GGGGTCGGGG AGGATGAGGA CCATCGAGGA 
AGGAGACTCA GCGACTTTTA TGACATCCAC CAGGAGATCG GCAGGGGTGC TTTCTCCTAC 
TTGCGGCGCA TAGTGGAGCG TAGCTCCGGC CTGGAGTTTG CGGCCAAGTT CATCCCCAGC 
CAGGCCAAGC CAAAGGCATC AGCGCGTCGG GAGGCCCGGC TGCTGGCCAG GCTCCAGCAC 
GACTGTGTCC TCTACTTCCA TGAGGCCTTC GAGAGGCGCC GGGGACTGGT CATTGTCACC 
GAGCTCTGCA CAGAGGAGCT GCTGGAGCGA ATCGCCAGGA AACCCACCGT GTGTGAGTCT 
GAGATCCGGG CCTATATGCG GCAGGTGCTA GAGGGAATAC ACTACCTGCA CCAGAGCCAC 
GTGCTGCACC TCGATGTCAA GCCTGAGAAC CTGCTGGTGT GGGATGGTGC TGCGGGCGAG 
CAGCAGGTGC GGATCTGTGA CTTTGGGAAT GCCCAGGAGC TGACTCCAGG AGAGCCCCAG 
TACTGCCAGT ATGGCACACC TGAGTTTGTA GCACCCGAGA TTGTCAATCA GAGCCCCGTG 
TCTGGAGTCA CTGACATCTG GCCTGTGGGT GTTGTTGCCT TCCTCTGTCT GACAGGAATC 
TCCCCGTTTG TTGGGGAAAA TGACCGGACA ACATTGATGA ACATCCGAAA CTACAACGTG 
GCCTTCGAGG AGACCACATT CCTGAGCCTG AGCAGGGAGG CCCGGGGCTT CCTCATCAAA 
GTGTTGGTGC AGGACCGGCT GAGACCTACC GCAGAAGAGA CCCTAGAACA TCCTTGGTTC 
AAAACTCAGG CAAAGGGCGC AGAGGTGAGC ACGGATCACC TGAAGCTATT CCTCTCCCGG 
CGGAGGTGGC AGCGCTCCCA GATCAGCTAC AAATGCCACC TGGTGCTGCG CCCCATCCCC 
GAGCTGCTGC GGGCCCCCCC AGAGCGGGTG TGGGTGACCA TGCCCAGAAG GCCACCCCCC 
AGTGGGGGGC TCTCATCCTC CTCGGATTCT GAAGAGGAAG AGCTGGAAGA GCTGCCCTCA 
GTGCCCCGCC CACTGCAGCC CGAGTTCTCT GGCTCCCGGG TGTCCCTCAC AGACATTCCC 
ACTGAGGATG AGGCCCTGGG GACCCCAGAG ACTGGGGCTG CCACCCCCAT GGACTGGCAG 
GAGCAGGGAA GGGCTCCCTC TCAGGACCAG GAGGCTCCCA GCCCAGAGGC CCTCCCCTCC 
CCAGGCCAGG AGCCCGCAGC TGGGGCTAGC CCCAGGCGGG GAGAGCTCCG CAGGGGCAGC 
TCGGCTGAGA GCGCCCTGCC CCGGGCCGGG CCGCGGGAGC TGGGCCGGGG CCTGCACAAG 
GCGGCGTCTG TGGAGCTGCC GCAGCGCCGG AGCCCCAGCC CGGGAGCCAC CCGCCTGGCC 
CGGGGAGGCC TGGGTGAGGG CGAGTATGCC CAGAGGCTGC AGGCCCTGCG CCAGCGGCTG 
CTGCGGGGAG GCCCCGAGGA TGGCAAGGTC AGCGGCCTCA GGGGTCCCCT GCTGGAGAGC 
CTGGGGGGCC GTGCTCGGGA CCCCCGGATG GCACGAGCTG CCTCCAGCGA GGCAGCGCCC 
CACCACCAGC CCCCACTCGA GAACCGGGGC CTGCAAAAGA GCAGCAGCTT CTCCCAGGGT 
GAGGCGGAGC CCCGGGGCCG GCACCGCCGA GCGGGGGCGC CCCTCGAGAT CCCCGTGGCC 
AGGCTTGGGG CCCGTAGGCT ACAGGAGTCT CCTTCCCTGT CTGCCCTCAG CGAGGCCCAG 
CCATCCAGCC CTGCACGGCC CAGCGCCCCC AAACCCAGTA CCCCTAAGTC TGCAGAACCT 
TCTGCCACCA CACCTAGTGA TGCTCCGCAG CCCCCCGCAC CCCAGCCTGC CCAAGACAAG 
GCTCCAGAGC CCAGGCCAGA ACCAGTCCGA GCCTCCAAGC CTGCACCACC CCCCCAGGCC 
CTGCAAACCC TAGCGCTGCC CCTCACACCC TATGCTCAGA TCATTCAGTC CCTCCAGCTG 
TCAGGCCACG CCCAGGGCCC CTCGCAGGGC CCTGCCGCGC CGCCTTCAGA GCCCAAGCCC 
CACGCTGCTG TCTTTGCCAG GGTGGCCTCC CCACCTCCGG GAGCCCCCGA GAAGCGCGTG 
CCCTCAGCCG GGGGTCCCCC GGTGCTAGCC GAGAAAGCCC GAGTTCCCAC GGTGCCCCCC 
AGGCCAGGCA GCAGTCTCAG TAGCAGCATC GAAAACTTGG AGTCGGAGGC CGTGTTCGAG 
GCCAAGTTCA AGCGCAGCCG CGAGTCGCCC CTGTCGCTGG GGCTGCGGCT GCTGAGCCGT 
TCGCGCTCGG AGGAGCGCGG CCCCTTCCGT GGGGCCGAGG AGGAGGATGG CATATACCGG 
CCCAGCCCGG CGGGGACCCC GCTGGAGCTG GTGCGACGGC CTGAGCGCTC ACGCTCGGTG 
CAGGACCTCA GGGCTGTCGG AGAGCCTGGC CTCGTCCGCC GCCTCTCGCT GTCACTGTCC 
CAGCGGCTGC GGCGGACCCC TCCCGCGCAG CGCCACCCGG CCTGGGAGGC CCGCGGCGGG 
GACGGAGAGA GCTCGGAGGG CGGGAGCTCG GCGCGGGGCT CCCCGGTGCT GGCGATGCGC 
AGGCGGCTGA GCTTCACCCT GGAGCGGCTG TCCAGCCGAT TGCAGCGCAG TGGCAGCAGC 
GAGGACTCGG GGGGCGCGTC GGGCCGCAGC ACGCCGCTGT TCGGACGGCT TCGCAGGGCC 
ACGTCCGAGG GCGAGAGTCT GCGGCGCCTT GGCCTTCCGC ACAACCAGTT GGCCGCCCAG 
GCCGGCGCCA CCACGCCTTC CGCCGAGTCC CTGGGCTCCG AGGCCAGCGC CACGTCGGGC 
TCCTCAGCCC CAGGGGAAAG CCGAAGCCGG CTCCGCTGGG GCTTCTCTCG GCCGCGGAAG 
GACAAGGGGT TATCGCCACC AAACCTCTCT GCCAGCGTCC AGGAGGAGTT GGGTCACCAG 
TACGTGCGCA GTGAGTCAGA CTTCCCCCCA GTCTTCCACA TCAAACTCAA GGACCAGGTG 
CTGCTGGAGG GGGAGGCAGC CACCCTGCTC TGCCTGCCAG CGGCCTGCCC TGCACCGCAC 
ATCTCCTGGA TGAAAGACAA GAAGTCCTTG AGGTCAGAGC CCTCAGTGAT CATCGTGTCC 
TGCAAAGATG GGCGGCAGCT GCTCAGCATC CCCCGGGCGG GCAAGCGGCA CGCCGGTCTC 
TATGAGTGCT CGGCCACCAA CGTACTGGGC AGCATCACCA GCTCCTGTAC CGTGGCTGTG 
GCCCGAGTCC CAGGAAAGCT AGCTCCTCCA GAGGTACCCC AGACCTACCA GGACACGGCG 
CTGGTGCTGT GGAAGCCGGG AGACAGCCGG GCACCTTGCA CGTATACGCT GGAGCGGCGA 
GTGGATGGGG AGTCTGTGTG GCACCCTGTG AGCTCAGGCA TCCCCGACTG TTACTACAAC 
GTGACCCACC TGCCAGTTGG CGTGACTGTG AGGTTCCGTG TGGCCTGTGC CAACCGTGCT 
GGGCAGGGGC CCTTCAGCAA CTCTTCTGAG AAGGTCTTTG TCAGGGGTAC TCAAGATTCT 
TCAGCTGTGC CATCTGCTGC CCACCAAGAG GCCCCTGTCA CCTCAAGGCC AGCCAGGGCC 
CGGCCTCCTG ACTCTCCTAC CTCACTGGCC CCACCCCTAG CTCCTGCTGC CCCCACACCC 
CCGTCAGTCA CTGTCAGCCC CTCATCTCCC CCCACACCTC CTAGCCAGGC CTTGTCCTCG 
CTCAAGGCTG TGGGTCCACC ACCCCAAACC CCTCCACGAA GACACAGGGG CCTGCAGGCT 
GCCCGGCCAG CGGAGCCCAC CCTACCCAGT ACCCACGTCA CCCCAAGTGA GCCCAAGCCT 
TTCGTCCTTG ACACTGGGAC CCCGATCCCA GCCTCCACTC CTCAAGGGGT TAAACCAGTG 
TCTTCCTCTA CTCCTGTGTA TGTGGTGACT TCCTTTGTGT CTGCACCACC AGCCCCTGAG 
CCCCCAGCCC CTGAGCCCCC TCCTGAGCCT ACCAAGGTGA CTGTGCAGAG CCTCAGCCCG 
GCCAAGGAGG TGGTCAGCTC CCCTGGGAGC AGTCCCCGAA GCTCTCCCAG GCCTGAGGGT 
ACCACTCTTC GACAGGGTCC CCCTCAGAAA CCCTACACCT TCCTGGAGGA GAAAGCCAGG 
GGCCGCTTTG GTGTTGTGCG AGCGTGCCGG GAGAATGCCA CGGGGCGAAC GTTCGTGGCC 
AAGATCGTGC CCTATGCTGC CGAGGGCAAG CGGCGGGTCC TGCAGGAGTA CGAGGTGCTG 
CGGACCCTGC ACCACGAGCG GATCATGTCC CTGCACGAGG CCTACATCAC CCCTCGGTAC 
CTCGTGCTCA TTGCTGAGAG CTGTGGCAAC CGGGAACTCC TCTGTGGGCT CAGTGACAGG 
TTCCGGTATT CTGAGGATGA CGTGGCCACT TACATGGTGC AGCTGCTACA AGGCCTGGAC 
TACCTCCACG GCCACCACGT GCTCCACCTA GACATCAAGC CAGACAACCT GCTGCTGGCC 
CCTGACAATG CCCTCAAGAT TGTGGACTTT GGCAGTGCCC AGCCCTACAA CCCCCAGGCC 
CTTAGGCCCC TTGGCCACCG CACGGGCACG CTGGAGTTCA TGGCTCCGGA GATGGTGAAG 
GGAGAACCCA TCGGCTCTGC CACGGACATC TGGGGAGCGG GTGTGCTCAC TTACATTATG 
CTCAGTGGAC GCTCCCCGTT CTATGAGCCA GACCCCCAGG AAACGGAGGC TCGGATTGTG 
GGGGGCCGCT TTGATGCCTT CCAGCTGTAC CCCAATACAT CCCAGAGCGC CACCCTCTTC 
TTGCGAAAGG TTCTCTCTGT ACATCCCTGG AGCCGGCCCT CCCTGCAGGA CTGCCTGGCC 
CACCCATGGT TGCAGGACGC CTACCTGATG AAGCTGCGCC GCCAGACGCT CACCTTCACC 
ACCAACCGGC TCAAGGAGTT CCTGGGCGAG CAGCGGCGGC GCCGGGCTGA GGCTGCCACC 
CGCCACAAGG TGCTGCTGCG CTCCTACCCT GGCGGCCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005246294.1
CDS	11..9532
Translation	MKKLWVKKRFQKTGHSRRAFGRLTHVFRSCRKQSYDSETAEDDISDVQGTQRLELRDDGAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRRLPGSPRQAQATGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSVPQSGLRREEPDLQPQLASEAPRRPAQPPPSKSALLPPPSPRVGKRSPPGPPAQPAATPTSPHRRTQEPVLPEDTTTEEKRGKKSKSSGPSLAGTAESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSERGAPWGTPGASQEELRAPGSVAERRRLFQQKAASLDERTRQRSPASDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAAAQPPSPSSAEKPGDEPGRPRSRGPAGRTEPGEGPQQEVRRRDQFPLTRSRAIQECRSPVPPPAADPPEARTKAPPGRKREPPAQAVRFLPWATPGLEGAAVPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGARSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNVVVAPGADVLLKCIITANPPPQVSWHKDGSALRSEGRLLLRAEGERHTLLLREARAADAGSYMATATNELGQATCAASLTVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPTDVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARFDCKISGTPPPVVTWTHFGCPMEESENLRLRQDGGLHSLHIAHVGSEDEGLYAVSAVNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEQGELERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDVHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPQVVAVTGRMVTLTWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPGWAATGLRKGVQHIFRVLSTTVKSSSKPSPPSEPVQLLEHGPTLEEAPAMLDKPDIVYVVEGQPASVTVTFNHVEAQVVWRSCRGALLEARAGVYELSQPDDDQYCLRICRVSRRDMGALTCTARNRHGTQTCSVTLELAEAPRFESIMEDVEVGAGETARFAVVVEGKPLPDIMWYKDEVLLTESSHVSFVYEENECSLVVLSTGAQDGGVYTCTAQNLAGEVSCKAELAVHSAQTAMEVEGVGEDEDHRGRRLSDFYDIHQEIGRGAFSYLRRIVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHDCVLYFHEAFERRRGLVIVTELCTEELLERIARKPTVCESEIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTQAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGELRRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQPPAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPSEPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEAKFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAVGEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAESLGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPHISWMKDKKSLRSEPSVIIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARVPGKLAPPEVPQTYQDTALVLWKPGDSRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTHLPVGVTVRFRVACANRAGQGPFSNSSEKVFVRGTQDSSAVPSAAHQEAPVTSRPARARPPDSPTSLAPPLAPAAPTPPSVTVSPSSPPTPPSQALSSLKAVGPPPQTPPRRHRGLQAARPAEPTLPSTHVTPSEPKPFVLDTGTPIPASTPQGVKPVSSSTPVYVVTSFVSAPPAPEPPAPEPPPEPTKVTVQSLSPAKEVVSSPGSSPRSSPRPEGTTLRQGPPQKPYTFLEEKARGRFGVVRACRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERIMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYMVQLLQGLDYLHGHHVLHLDIKPDNLLLAPDNALKIVDFGSAQPYNPQALRPLGHRTGTLEFMAPEMVKGEPIGSATDIWGAGVLTYIMLSGRSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGGP

Target ORF information:

RefSeq Version	XM_005246237.2
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246237.2

ORF Insert Sequence:

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTCA CCCATGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC AGAGACGGCT 
GAGGATGACA TCAGCGATGT GCAGGGAACC CAGCGCCTGG AGCTTCGGGA TGACGGGGCC 
TTCAGCACCC CCACGGGGGG TTCTGACACC CTGGTGGGCA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG TGGGGGTGGC ACCCGCCGCC TCCCGGGCAG CCCAAGGCAA 
GCACAGGCAA CCGGGGCCGG GCCACGGCAC CTGGGGGTGG AGCCGCTGGT GCGGGCATCT 
CGAGCTAATC TGGTGGGCGC AAGCTGGGGG TCAGAGGATA GCCTTTCCGT GGCCAGTGAC 
CTGTACGGCA GCGCATTCAG CCTGTACAGA GGACGGGCGC TCTCTATCCA CGTCAGCGTC 
CCTCAGAGCG GGTTGCGCAG GGAGGAGCCC GACCTTCAGC CTCAACTGGC CAGCGAAGCC 
CCACGCCGCC CTGCCCAGCC GCCTCCTTCC AAATCCGCGC TGCTCCCCCC ACCGTCCCCT 
CGGGTCGGGA AGCGGTCCCC GCCGGGACCC CCGGCCCAGC CCGCGGCCAC CCCCACGTCG 
CCCCACCGTC GCACTCAGGA GCCTGTGCTG CCCGAGGACA CCACCACCGA AGAGAAGCGA 
GGGAAGAAGT CCAAGTCGTC CGGGCCCTCC CTGGCGGGCA CCGCGGAATC CCGACCCCAG 
ACGCCACTGA GCGAGGCCTC AGGCCGCCTG TCGGCGTTGG GCCGATCGCC TAGGCTGGTG 
CGCGCCGGCT CCCGCATCCT GGACAAGCTG CAGTTCTTCG AGGAGCGACG GCGCAGCCTG 
GAGCGCAGCG ACTCGCCGCC GGCGCCCCTG CGGCCCTGGG TGCCCCTGCG CAAGGCCCGC 
TCTCTGGAGC AGCCCAAGTC GGAGCGCGGC GCACCGTGGG GCACCCCCGG GGCCTCGCAG 
GAAGAACTGC GGGCGCCAGG CAGCGTGGCC GAGCGGCGCC GCCTGTTCCA GCAGAAAGCG 
GCCTCGCTGG ACGAGCGCAC GCGTCAGCGC AGCCCGGCCT CAGACCTCGA GCTGCGCTTC 
GCCCAGGAGC TGGGCCGCAT CCGCCGCTCC ACGTCGCGGG AGGAGCTGGT GCGCTCGCAC 
GAGTCCCTGC GCGCCACGCT GCAGCGTGCC CCATCCCCTC GAGAGCCCGG CGAGCCCCCG 
CTCTTCTCTC GGCCCTCCAC CCCCAAGACA TCGCGGGCCG TGAGCCCCGC CGCCGCCCAG 
CCGCCCTCTC CGAGCAGCGC GGAGAAGCCG GGGGACGAGC CTGGGAGGCC CAGGAGCCGC 
GGGCCGGCGG GCAGGACAGA GCCGGGGGAA GGCCCGCAGC AGGAGGTTAG GCGTCGGGAC 
CAATTCCCGC TGACCCGGAG CAGAGCCATC CAGGAGTGCA GGAGCCCTGT GCCGCCCCCC 
GCCGCCGATC CCCCAGAGGC CAGGACGAAA GCACCCCCCG GTCGGAAGCG GGAGCCCCCG 
GCGCAGGCCG TGCGCTTCCT GCCCTGGGCC ACGCCGGGCC TGGAGGGCGC TGCTGTACCC 
CAGACCTTGG AGAAGAACAG GGCGGGGCCT GAGGCAGAGA AGAGGCTTCG CAGAGGGCCG 
GAGGAGGACG GTCCCTGGGG GCCCTGGGAC CGCCGAGGGG CCCGCAGCCA GGGCAAAGGT 
CGCCGGGCCC GGCCCACCTC CCCTGAGCTC GAGTCTTCGG ATGACTCCTA CGTGTCCGCT 
GGAGAAGAGC CCCTAGAGGC CCCTGTGTTT GAGATCCCCC TGCAGAATGT GGTGGTGGCA 
CCAGGGGCAG ATGTGCTGCT CAAGTGTATC ATCACTGCCA ACCCCCCGCC CCAAGTGTCC 
TGGCACAAGG ATGGGTCAGC GCTGCGCAGC GAGGGCCGCC TCCTCCTCCG GGCTGAGGGT 
GAGCGGCACA CCCTGCTGCT CAGGGAGGCC AGGGCAGCAG ATGCCGGGAG CTATATGGCC 
ACCGCCACCA ACGAGCTGGG CCAGGCCACC TGTGCCGCCT CACTGACCGT GAGACCCGGT 
GGGTCTACAT CCCCTTTCAG CAGCCCCATC ACCTCCGACG AGGAATACCT GAGCCCCCCA 
GAGGAGTTCC CAGAGCCTGG GGAGACCTGG CCGCGAACCC CCACCATGAA GCCCAGTCCC 
AGCCAGAACC GCCGTTCTTC TGACACTGGC TCCAAGGCAC CCCCCACCTT CAAGGTCTCA 
CTTATGGACC AGTCAGTAAG AGAAGGCCAA GATGTCATCA TGAGCATCCG CGTGCAGGGG 
GAGCCCAAGC CTGTGGTCTC CTGGCTGAGA AACCGCCAGC CCGTGCGCCC AGACCAGCGG 
CGCTTTGCGG AGGAGGCTGA GGGTGGGCTG TGCCGGCTGC GGATCCTGGC TGCAGAGCGT 
GGCGATGCTG GTTTCTACAC TTGCAAAGCG GTCAATGAGT ATGGTGCTCG GCAGTGCGAG 
GCCCGCTTGG AGGTCCGAGC ACACCCTGAA AGCCGGTCCC TGGCCGTGCT GGCCCCCCTG 
CAGGACGTGG ACGTGGGGGC CGGGGAGATG GCGCTGTTTG AGTGCCTGGT GGCGGGGCCC 
ACTGACGTGG AGGTGGATTG GCTGTGCCGT GGCCGCCTGC TGCAGCCTGC ACTGCTCAAA 
TGCAAGATGC ATTTCGATGG CCGCAAATGC AAGCTGCTAC TTACATCTGT ACATGAGGAC 
GACAGTGGCG TCTACACCTG CAAGCTCAGC ACGGCCAAAG ATGAGCTGAC CTGCAGTGCC 
CGGCTGACCG TGCGGCCCTC GTTGGCACCC CTGTTCACAC GGCTGCTGGA AGATGTGGAG 
GTGTTGGAGG GCCGAGCTGC CCGTTTCGAC TGCAAGATCA GTGGCACCCC GCCCCCTGTT 
GTTACCTGGA CTCATTTTGG CTGCCCCATG GAGGAGAGTG AGAACTTGCG GCTGCGGCAG 
GACGGGGGTC TGCACTCACT GCACATTGCC CATGTGGGCA GCGAGGACGA GGGGCTCTAT 
GCGGTCAGTG CTGTTAACAC CCATGGCCAG GCCCACTGCT CAGCCCAGCT GTATGTAGAA 
GAGCCCCGGA CAGCCGCCTC AGGCCCCAGC TCGAAGCTGG AGAAGATGCC ATCCATTCCC 
GAGGAGCCAG AGCAGGGTGA GCTGGAGCGG CTGTCCATTC CTGACTTCCT GCGGCCACTG 
CAGGACCTGG AGGTGGGACT GGCCAAGGAG GCCATGCTAG AGTGCCAGGT GACCGGCCTG 
CCCTACCCCA CCATCAGCTG GTTCCACAAT GGCCACCGCA TCCAGAGCAG CGACGACCGG 
CGCATGACAC AGTACAGGGA TGTCCATCGC TTGGTGTTCC CTGCCGTGGG GCCTCAGCAC 
GCCGGTGTCT ACAAGAGCGT CATTGCCAAC AAGCTGGGCA AAGCTGCCTG CTATGCCCAC 
CTGTATGTCA CAGATGTGGT CCCAGGCCCT CCAGATGGCG CCCCGCAGGT GGTGGCTGTG 
ACGGGGAGGA TGGTCACACT CACATGGAAC CCCCCCAGGA GTCTGGACAT GGCCATCGAC 
CCGGACTCCC TGACGTACAC AGTGCAGCAC CAGGTGCTGG GCTCGGACCA GTGGACGGCA 
CTGGTCACAG GCCTGCGGGA GCCAGGGTGG GCAGCCACAG GGCTGCGTAA GGGGGTCCAG 
CACATCTTCC GGGTCCTCAG CACCACTGTC AAGAGCAGCA GCAAGCCCTC ACCCCCTTCT 
GAGCCTGTGC AGCTGCTGGA GCACGGCCCA ACCCTGGAGG AGGCCCCTGC CATGCTGGAC 
AAACCAGACA TCGTGTATGT GGTGGAGGGA CAGCCTGCCA GCGTCACCGT CACATTCAAC 
CATGTGGAGG CCCAGGTCGT CTGGAGGAGC TGCCGAGGGG CCCTCCTAGA GGCACGGGCC 
GGTGTGTACG AGCTGAGCCA GCCAGATGAT GACCAGTACT GTCTTCGGAT CTGCCGGGTG 
AGCCGCCGGG ACATGGGGGC CCTCACCTGC ACCGCCCGAA ACCGTCACGG CACACAGACC 
TGCTCGGTCA CATTGGAGCT GGCAGAGGCC CCTCGGTTTG AGTCCATCAT GGAGGACGTG 
GAGGTGGGGG CTGGGGAAAC TGCTCGCTTT GCGGTGGTGG TCGAGGGAAA ACCACTGCCG 
GACATCATGT GGTACAAGGA CGAGGTGCTG CTGACCGAGA GCAGCCATGT GAGCTTCGTG 
TACGAGGAGA ATGAGTGCTC CCTGGTGGTG CTCAGCACGG GGGCCCAGGA TGGAGGCGTC 
TACACCTGCA CCGCCCAGAA CCTGGCGGGT GAGGTCTCCT GCAAAGCAGA GTTGGCTGTG 
CATTCAGCTC AGACAGCTAT GGAGGTCGAG GGGGTCGGGG AGGATGAGGA CCATCGAGGA 
AGGAGACTCA GCGACTTTTA TGACATCCAC CAGGAGATCG GCAGGGGTGC TTTCTCCTAC 
TTGCGGCGCA TAGTGGAGCG TAGCTCCGGC CTGGAGTTTG CGGCCAAGTT CATCCCCAGC 
CAGGCCAAGC CAAAGGCATC AGCGCGTCGG GAGGCCCGGC TGCTGGCCAG GCTCCAGCAC 
GACTGTGTCC TCTACTTCCA TGAGGCCTTC GAGAGGCGCC GGGGACTGGT CATTGTCACC 
GAGCTCTGCA CAGAGGAGCT GCTGGAGCGA ATCGCCAGGA AACCCACCGT GTGTGAGTCT 
GAGATCCGGG CCTATATGCG GCAGGTGCTA GAGGGAATAC ACTACCTGCA CCAGAGCCAC 
GTGCTGCACC TCGATGTCAA GCCTGAGAAC CTGCTGGTGT GGGATGGTGC TGCGGGCGAG 
CAGCAGGTGC GGATCTGTGA CTTTGGGAAT GCCCAGGAGC TGACTCCAGG AGAGCCCCAG 
TACTGCCAGT ATGGCACACC TGAGTTTGTA GCACCCGAGA TTGTCAATCA GAGCCCCGTG 
TCTGGAGTCA CTGACATCTG GCCTGTGGGT GTTGTTGCCT TCCTCTGTCT GACAGGAATC 
TCCCCGTTTG TTGGGGAAAA TGACCGGACA ACATTGATGA ACATCCGAAA CTACAACGTG 
GCCTTCGAGG AGACCACATT CCTGAGCCTG AGCAGGGAGG CCCGGGGCTT CCTCATCAAA 
GTGTTGGTGC AGGACCGGCT GAGACCTACC GCAGAAGAGA CCCTAGAACA TCCTTGGTTC 
AAAACTCAGG CAAAGGGCGC AGAGGTGAGC ACGGATCACC TGAAGCTATT CCTCTCCCGG 
CGGAGGTGGC AGCGCTCCCA GATCAGCTAC AAATGCCACC TGGTGCTGCG CCCCATCCCC 
GAGCTGCTGC GGGCCCCCCC AGAGCGGGTG TGGGTGACCA TGCCCAGAAG GCCACCCCCC 
AGTGGGGGGC TCTCATCCTC CTCGGATTCT GAAGAGGAAG AGCTGGAAGA GCTGCCCTCA 
GTGCCCCGCC CACTGCAGCC CGAGTTCTCT GGCTCCCGGG TGTCCCTCAC AGACATTCCC 
ACTGAGGATG AGGCCCTGGG GACCCCAGAG ACTGGGGCTG CCACCCCCAT GGACTGGCAG 
GAGCAGGGAA GGGCTCCCTC TCAGGACCAG GAGGCTCCCA GCCCAGAGGC CCTCCCCTCC 
CCAGGCCAGG AGCCCGCAGC TGGGGCTAGC CCCAGGCGGG GAGAGCTCCG CAGGGGCAGC 
TCGGCTGAGA GCGCCCTGCC CCGGGCCGGG CCGCGGGAGC TGGGCCGGGG CCTGCACAAG 
GCGGCGTCTG TGGAGCTGCC GCAGCGCCGG AGCCCCAGCC CGGGAGCCAC CCGCCTGGCC 
CGGGGAGGCC TGGGTGAGGG CGAGTATGCC CAGAGGCTGC AGGCCCTGCG CCAGCGGCTG 
CTGCGGGGAG GCCCCGAGGA TGGCAAGGTC AGCGGCCTCA GGGGTCCCCT GCTGGAGAGC 
CTGGGGGGCC GTGCTCGGGA CCCCCGGATG GCACGAGCTG CCTCCAGCGA GGCAGCGCCC 
CACCACCAGC CCCCACTCGA GAACCGGGGC CTGCAAAAGA GCAGCAGCTT CTCCCAGGGT 
GAGGCGGAGC CCCGGGGCCG GCACCGCCGA GCGGGGGCGC CCCTCGAGAT CCCCGTGGCC 
AGGCTTGGGG CCCGTAGGCT ACAGGAGTCT CCTTCCCTGT CTGCCCTCAG CGAGGCCCAG 
CCATCCAGCC CTGCACGGCC CAGCGCCCCC AAACCCAGTA CCCCTAAGTC TGCAGAACCT 
TCTGCCACCA CACCTAGTGA TGCTCCGCAG CCCCCCGCAC CCCAGCCTGC CCAAGACAAG 
GCTCCAGAGC CCAGGCCAGA ACCAGTCCGA GCCTCCAAGC CTGCACCACC CCCCCAGGCC 
CTGCAAACCC TAGCGCTGCC CCTCACACCC TATGCTCAGA TCATTCAGTC CCTCCAGCTG 
TCAGGCCACG CCCAGGGCCC CTCGCAGGGC CCTGCCGCGC CGCCTTCAGA GCCCAAGCCC 
CACGCTGCTG TCTTTGCCAG GGTGGCCTCC CCACCTCCGG GAGCCCCCGA GAAGCGCGTG 
CCCTCAGCCG GGGGTCCCCC GGTGCTAGCC GAGAAAGCCC GAGTTCCCAC GGTGCCCCCC 
AGGCCAGGCA GCAGTCTCAG TAGCAGCATC GAAAACTTGG AGTCGGAGGC CGTGTTCGAG 
GCCAAGTTCA AGCGCAGCCG CGAGTCGCCC CTGTCGCTGG GGCTGCGGCT GCTGAGCCGT 
TCGCGCTCGG AGGAGCGCGG CCCCTTCCGT GGGGCCGAGG AGGAGGATGG CATATACCGG 
CCCAGCCCGG CGGGGACCCC GCTGGAGCTG GTGCGACGGC CTGAGCGCTC ACGCTCGGTG 
CAGGACCTCA GGGCTGTCGG AGAGCCTGGC CTCGTCCGCC GCCTCTCGCT GTCACTGTCC 
CAGCGGCTGC GGCGGACCCC TCCCGCGCAG CGCCACCCGG CCTGGGAGGC CCGCGGCGGG 
GACGGAGAGA GCTCGGAGGG CGGGAGCTCG GCGCGGGGCT CCCCGGTGCT GGCGATGCGC 
AGGCGGCTGA GCTTCACCCT GGAGCGGCTG TCCAGCCGAT TGCAGCGCAG TGGCAGCAGC 
GAGGACTCGG GGGGCGCGTC GGGCCGCAGC ACGCCGCTGT TCGGACGGCT TCGCAGGGCC 
ACGTCCGAGG GCGAGAGTCT GCGGCGCCTT GGCCTTCCGC ACAACCAGTT GGCCGCCCAG 
GCCGGCGCCA CCACGCCTTC CGCCGAGTCC CTGGGCTCCG AGGCCAGCGC CACGTCGGGC 
TCCTCAGCCC CAGGGGAAAG CCGAAGCCGG CTCCGCTGGG GCTTCTCTCG GCCGCGGAAG 
GACAAGGGGT TATCGCCACC AAACCTCTCT GCCAGCGTCC AGGAGGAGTT GGGTCACCAG 
TACGTGCGCA GTGAGTCAGA CTTCCCCCCA GTCTTCCACA TCAAACTCAA GGACCAGGTG 
CTGCTGGAGG GGGAGGCAGC CACCCTGCTC TGCCTGCCAG CGGCCTGCCC TGCACCGCAC 
ATCTCCTGGA TGAAAGACAA GAAGTCCTTG AGGTCAGAGC CCTCAGTGAT CATCGTGTCC 
TGCAAAGATG GGCGGCAGCT GCTCAGCATC CCCCGGGCGG GCAAGCGGCA CGCCGGTCTC 
TATGAGTGCT CGGCCACCAA CGTACTGGGC AGCATCACCA GCTCCTGTAC CGTGGCTGTG 
GCCCGAGTCC CAGGAAAGCT AGCTCCTCCA GAGGTACCCC AGACCTACCA GGACACGGCG 
CTGGTGCTGT GGAAGCCGGG AGACAGCCGG GCACCTTGCA CGTATACGCT GGAGCGGCGA 
GTGGATGGGG AGTCTGTGTG GCACCCTGTG AGCTCAGGCA TCCCCGACTG TTACTACAAC 
GTGACCCACC TGCCAGTTGG CGTGACTGTG AGGTTCCGTG TGGCCTGTGC CAACCGTGCT 
GGGCAGGGGC CCTTCAGCAA CTCTTCTGAG AAGGTCTTTG TCAGGGGTAC TCAAGATTCT 
TCAGCTGTGC CATCTGCTGC CCACCAAGAG GCCCCTGTCA CCTCAAGGCC AGCCAGGGCC 
CGGCCTCCTG ACTCTCCTAC CTCACTGGCC CCACCCCTAG CTCCTGCTGC CCCCACACCC 
CCGTCAGTCA CTGTCAGCCC CTCATCTCCC CCCACACCTC CTAGCCAGGC CTTGTCCTCG 
CTCAAGGCTG TGGGTCCACC ACCCCAAACC CCTCCACGAA GACACAGGGG CCTGCAGGCT 
GCCCGGCCAG CGGAGCCCAC CCTACCCAGT ACCCACGTCA CCCCAAGTGA GCCCAAGCCT 
TTCGTCCTTG ACACTGGGAC CCCGATCCCA GCCTCCACTC CTCAAGGGGT TAAACCAGTG 
TCTTCCTCTA CTCCTGTGTA TGTGGTGACT TCCTTTGTGT CTGCACCACC AGCCCCTGAG 
CCCCCAGCCC CTGAGCCCCC TCCTGAGCCT ACCAAGGTGA CTGTGCAGAG CCTCAGCCCG 
GCCAAGGAGG TGGTCAGCTC CCCTGGGAGC AGTCCCCGAA GCTCTCCCAG GCCTGAGGGT 
ACCACTCTTC GACAGGGTCC CCCTCAGAAA CCCTACACCT TCCTGGAGGA GAAAGCCAGG 
GGCCGCTTTG GTGTTGTGCG AGCGTGCCGG GAGAATGCCA CGGGGCGAAC GTTCGTGGCC 
AAGATCGTGC CCTATGCTGC CGAGGGCAAG CGGCGGGTCC TGCAGGAGTA CGAGGTGCTG 
CGGACCCTGC ACCACGAGCG GATCATGTCC CTGCACGAGG CCTACATCAC CCCTCGGTAC 
CTCGTGCTCA TTGCTGAGAG CTGTGGCAAC CGGGAACTCC TCTGTGGGCT CAGTGACAGG 
TTCCGGTATT CTGAGGATGA CGTGGCCACT TACATGGTGC AGCTGCTACA AGGCCTGGAC 
TACCTCCACG GCCACCACGT GCTCCACCTA GACATCAAGC CAGACAACCT GCTGCTGGCC 
CCTGACAATG CCCTCAAGAT TGTGGACTTT GGCAGTGCCC AGCCCTACAA CCCCCAGGCC 
CTTAGGCCCC TTGGCCACCG CACGGGCACG CTGGAGTTCA TGGCTCCGGA GATGGTGAAG 
GGAGAACCCA TCGGCTCTGC CACGGACATC TGGGGAGCGG GTGTGCTCAC TTACATTATG 
CTCAGTGGAC GCTCCCCGTT CTATGAGCCA GACCCCCAGG AAACGGAGGC TCGGATTGTG 
GGGGGCCGCT TTGATGCCTT CCAGCTGTAC CCCAATACAT CCCAGAGCGC CACCCTCTTC 
TTGCGAAAGG TTCTCTCTGT ACATCCCTGG AGCCGGCCCT CCCTGCAGGA CTGCCTGGCC 
CACCCATGGT TGCAGGACGC CTACCTGATG AAGCTGCGCC GCCAGACGCT CACCTTCACC 
ACCAACCGGC TCAAGGAGTT CCTGGGCGAG CAGCGGCGGC GCCGGGCTGA GGCTGCCACC 
CGCCACAAGG TGCTGCTGCG CTCCTACCCT GGCGGCCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_006712189.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712252.1 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X6, mRNA.

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CloneID	OHu35752
Clone ID Related Accession (Same CDS sequence)	XM_006712189.3
Accession Version	XM_006712189.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712189.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTCA CCCATGACTC AGAGACGGCT GAGGATGACA TCAGCGATGT GCAGGGAACC 
CAGCGCCTGG AGCTTCGGGA TGACGGGGCC TTCAGCACCC CCACGGGGGG TTCTGACACC 
CTGGTGGGCA CCTCCCTGGA CACACCCCCG ACCTCCGTGA CAGGCACCTC AGAGGAGCAA 
GTGAGCTGGT GGGGCAGCGG GCAGACGGTC CTGGAGCAGG AAGCGGGCAG TGGGGGTGGC 
ACCCGCCGCC TCCCGGGCAG CCCAAGGCAA GCACAGGCAA CCGGGGCCGG GCCACGGCAC 
CTGGGGGTGG AGCCGCTGGT GCGGGCATCT CGAGCTAATC TGGTGGGCGC AAGCTGGGGG 
TCAGAGGATA GCCTTTCCGT GGCCAGTGAC CTGTACGGCA GCGCATTCAG CCTGTACAGA 
GGACGGGCGC TCTCTATCCA CGTCAGCGTC CCTCAGAGCG GGTTGCGCAG GGAGGAGCCC 
GACCTTCAGC CTCAACTGGC CAGCGAAGCC CCACGCCGCC CTGCCCAGCC GCCTCCTTCC 
AAATCCGCGC TGCTCCCCCC ACCGTCCCCT CGGGTCGGGA AGCGGTCCCC GCCGGGACCC 
CCGGCCCAGC CCGCGGCCAC CCCCACGTCG CCCCACCGTC GCACTCAGGA GCCTGTGCTG 
CCCGAGGACA CCACCACCGA AGAGAAGCGA GGGAAGAAGT CCAAGTCGTC CGGGCCCTCC 
CTGGCGGGCA CCGCGGAATC CCGACCCCAG ACGCCACTGA GCGAGGCCTC AGGCCGCCTG 
TCGGCGTTGG GCCGATCGCC TAGGCTGGTG CGCGCCGGCT CCCGCATCCT GGACAAGCTG 
CAGTTCTTCG AGGAGCGACG GCGCAGCCTG GAGCGCAGCG ACTCGCCGCC GGCGCCCCTG 
CGGCCCTGGG TGCCCCTGCG CAAGGCCCGC TCTCTGGAGC AGCCCAAGTC GGAGCGCGGC 
GCACCGTGGG GCACCCCCGG GGCCTCGCAG GAAGAACTGC GGGCGCCAGG CAGCGTGGCC 
GAGCGGCGCC GCCTGTTCCA GCAGAAAGCG GCCTCGCTGG ACGAGCGCAC GCGTCAGCGC 
AGCCCGGCCT CAGACCTCGA GCTGCGCTTC GCCCAGGAGC TGGGCCGCAT CCGCCGCTCC 
ACGTCGCGGG AGGAGCTGGT GCGCTCGCAC GAGTCCCTGC GCGCCACGCT GCAGCGTGCC 
CCATCCCCTC GAGAGCCCGG CGAGCCCCCG CTCTTCTCTC GGCCCTCCAC CCCCAAGACA 
TCGCGGGCCG TGAGCCCCGC CGCCGCCCAG CCGCCCTCTC CGAGCAGCGC GGAGAAGCCG 
GGGGACGAGC CTGGGAGGCC CAGGAGCCGC GGGCCGGCGG GCAGGACAGA GCCGGGGGAA 
GGCCCGCAGC AGGAGGTTAG GCGTCGGGAC CAATTCCCGC TGACCCGGAG CAGAGCCATC 
CAGGAGTGCA GGAGCCCTGT GCCGCCCCCC GCCGCCGATC CCCCAGAGGC CAGGACGAAA 
GCACCCCCCG GTCGGAAGCG GGAGCCCCCG GCGCAGGCCG TGCGCTTCCT GCCCTGGGCC 
ACGCCGGGCC TGGAGGGCGC TGCTGTACCC CAGACCTTGG AGAAGAACAG GGCGGGGCCT 
GAGGCAGAGA AGAGGCTTCG CAGAGGGCCG GAGGAGGACG GTCCCTGGGG GCCCTGGGAC 
CGCCGAGGGG CCCGCAGCCA GGGCAAAGGT CGCCGGGCCC GGCCCACCTC CCCTGAGCTC 
GAGTCTTCGG ATGACTCCTA CGTGTCCGCT GGAGAAGAGC CCCTAGAGGC CCCTGTGTTT 
GAGATCCCCC TGCAGAATGT GGTGGTGGCA CCAGGGGCAG ATGTGCTGCT CAAGTGTATC 
ATCACTGCCA ACCCCCCGCC CCAAGTGTCC TGGCACAAGG ATGGGTCAGC GCTGCGCAGC 
GAGGGCCGCC TCCTCCTCCG GGCTGAGGGT GAGCGGCACA CCCTGCTGCT CAGGGAGGCC 
AGGGCAGCAG ATGCCGGGAG CTATATGGCC ACCGCCACCA ACGAGCTGGG CCAGGCCACC 
TGTGCCGCCT CACTGACCGT GAGACCCGGT GGGTCTACAT CCCCTTTCAG CAGCCCCATC 
ACCTCCGACG AGGAATACCT GAGCCCCCCA GAGGAGTTCC CAGAGCCTGG GGAGACCTGG 
CCGCGAACCC CCACCATGAA GCCCAGTCCC AGCCAGAACC GCCGTTCTTC TGACACTGGC 
TCCAAGGCAC CCCCCACCTT CAAGGTCTCA CTTATGGACC AGTCAGTAAG AGAAGGCCAA 
GATGTCATCA TGAGCATCCG CGTGCAGGGG GAGCCCAAGC CTGTGGTCTC CTGGCTGAGA 
AACCGCCAGC CCGTGCGCCC AGACCAGCGG CGCTTTGCGG AGGAGGCTGA GGGTGGGCTG 
TGCCGGCTGC GGATCCTGGC TGCAGAGCGT GGCGATGCTG GTTTCTACAC TTGCAAAGCG 
GTCAATGAGT ATGGTGCTCG GCAGTGCGAG GCCCGCTTGG AGGTCCGAGC ACACCCTGAA 
AGCCGGTCCC TGGCCGTGCT GGCCCCCCTG CAGGACGTGG ACGTGGGGGC CGGGGAGATG 
GCGCTGTTTG AGTGCCTGGT GGCGGGGCCC ACTGACGTGG AGGTGGATTG GCTGTGCCGT 
GGCCGCCTGC TGCAGCCTGC ACTGCTCAAA TGCAAGATGC ATTTCGATGG CCGCAAATGC 
AAGCTGCTAC TTACATCTGT ACATGAGGAC GACAGTGGCG TCTACACCTG CAAGCTCAGC 
ACGGCCAAAG ATGAGCTGAC CTGCAGTGCC CGGCTGACCG TGCGGCCCTC GTTGGCACCC 
CTGTTCACAC GGCTGCTGGA AGATGTGGAG GTGTTGGAGG GCCGAGCTGC CCGTTTCGAC 
TGCAAGATCA GTGGCACCCC GCCCCCTGTT GTTACCTGGA CTCATTTTGG CTGCCCCATG 
GAGGAGAGTG AGAACTTGCG GCTGCGGCAG GACGGGGGTC TGCACTCACT GCACATTGCC 
CATGTGGGCA GCGAGGACGA GGGGCTCTAT GCGGTCAGTG CTGTTAACAC CCATGGCCAG 
GCCCACTGCT CAGCCCAGCT GTATGTAGAA GAGCCCCGGA CAGCCGCCTC AGGCCCCAGC 
TCGAAGCTGG AGAAGATGCC ATCCATTCCC GAGGAGCCAG AGCAGGGTGA GCTGGAGCGG 
CTGTCCATTC CTGACTTCCT GCGGCCACTG CAGGACCTGG AGGTGGGACT GGCCAAGGAG 
GCCATGCTAG AGTGCCAGGT GACCGGCCTG CCCTACCCCA CCATCAGCTG GTTCCACAAT 
GGCCACCGCA TCCAGAGCAG CGACGACCGG CGCATGACAC AGTACAGGGA TGTCCATCGC 
TTGGTGTTCC CTGCCGTGGG GCCTCAGCAC GCCGGTGTCT ACAAGAGCGT CATTGCCAAC 
AAGCTGGGCA AAGCTGCCTG CTATGCCCAC CTGTATGTCA CAGATGTGGT CCCAGGCCCT 
CCAGATGGCG CCCCGCAGGT GGTGGCTGTG ACGGGGAGGA TGGTCACACT CACATGGAAC 
CCCCCCAGGA GTCTGGACAT GGCCATCGAC CCGGACTCCC TGACGTACAC AGTGCAGCAC 
CAGGTGCTGG GCTCGGACCA GTGGACGGCA CTGGTCACAG GCCTGCGGGA GCCAGGGTGG 
GCAGCCACAG GGCTGCGTAA GGGGGTCCAG CACATCTTCC GGGTCCTCAG CACCACTGTC 
AAGAGCAGCA GCAAGCCCTC ACCCCCTTCT GAGCCTGTGC AGCTGCTGGA GCACGGCCCA 
ACCCTGGAGG AGGCCCCTGC CATGCTGGAC AAACCAGACA TCGTGTATGT GGTGGAGGGA 
CAGCCTGCCA GCGTCACCGT CACATTCAAC CATGTGGAGG CCCAGGTCGT CTGGAGGAGC 
TGCCGAGGGG CCCTCCTAGA GGCACGGGCC GGTGTGTACG AGCTGAGCCA GCCAGATGAT 
GACCAGTACT GTCTTCGGAT CTGCCGGGTG AGCCGCCGGG ACATGGGGGC CCTCACCTGC 
ACCGCCCGAA ACCGTCACGG CACACAGACC TGCTCGGTCA CATTGGAGCT GGCAGAGGCC 
CCTCGGTTTG AGTCCATCAT GGAGGACGTG GAGGTGGGGG CTGGGGAAAC TGCTCGCTTT 
GCGGTGGTGG TCGAGGGAAA ACCACTGCCG GACATCATGT GGTACAAGGA CGAGGTGCTG 
CTGACCGAGA GCAGCCATGT GAGCTTCGTG TACGAGGAGA ATGAGTGCTC CCTGGTGGTG 
CTCAGCACGG GGGCCCAGGA TGGAGGCGTC TACACCTGCA CCGCCCAGAA CCTGGCGGGT 
GAGGTCTCCT GCAAAGCAGA GTTGGCTGTG CATTCAGCTC AGACAGCTAT GGAGGTCGAG 
GGGGTCGGGG AGGATGAGGA CCATCGAGGA AGGAGACTCA GCGACTTTTA TGACATCCAC 
CAGGAGATCG GCAGGGGTGC TTTCTCCTAC TTGCGGCGCA TAGTGGAGCG TAGCTCCGGC 
CTGGAGTTTG CGGCCAAGTT CATCCCCAGC CAGGCCAAGC CAAAGGCATC AGCGCGTCGG 
GAGGCCCGGC TGCTGGCCAG GCTCCAGCAC GACTGTGTCC TCTACTTCCA TGAGGCCTTC 
GAGAGGCGCC GGGGACTGGT CATTGTCACC GAGCTCTGCA CAGAGGAGCT GCTGGAGCGA 
ATCGCCAGGA AACCCACCGT GTGTGAGTCT GAGATCCGGG CCTATATGCG GCAGGTGCTA 
GAGGGAATAC ACTACCTGCA CCAGAGCCAC GTGCTGCACC TCGATGTCAA GCCTGAGAAC 
CTGCTGGTGT GGGATGGTGC TGCGGGCGAG CAGCAGGTGC GGATCTGTGA CTTTGGGAAT 
GCCCAGGAGC TGACTCCAGG AGAGCCCCAG TACTGCCAGT ATGGCACACC TGAGTTTGTA 
GCACCCGAGA TTGTCAATCA GAGCCCCGTG TCTGGAGTCA CTGACATCTG GCCTGTGGGT 
GTTGTTGCCT TCCTCTGTCT GACAGGAATC TCCCCGTTTG TTGGGGAAAA TGACCGGACA 
ACATTGATGA ACATCCGAAA CTACAACGTG GCCTTCGAGG AGACCACATT CCTGAGCCTG 
AGCAGGGAGG CCCGGGGCTT CCTCATCAAA GTGTTGGTGC AGGACCGGCT GAGACCTACC 
GCAGAAGAGA CCCTAGAACA TCCTTGGTTC AAAACTCAGG CAAAGGGCGC AGAGGTGAGC 
ACGGATCACC TGAAGCTATT CCTCTCCCGG CGGAGGTGGC AGCGCTCCCA GATCAGCTAC 
AAATGCCACC TGGTGCTGCG CCCCATCCCC GAGCTGCTGC GGGCCCCCCC AGAGCGGGTG 
TGGGTGACCA TGCCCAGAAG GCCACCCCCC AGTGGGGGGC TCTCATCCTC CTCGGATTCT 
GAAGAGGAAG AGCTGGAAGA GCTGCCCTCA GTGCCCCGCC CACTGCAGCC CGAGTTCTCT 
GGCTCCCGGG TGTCCCTCAC AGACATTCCC ACTGAGGATG AGGCCCTGGG GACCCCAGAG 
ACTGGGGCTG CCACCCCCAT GGACTGGCAG GAGCAGGGAA GGGCTCCCTC TCAGGACCAG 
GAGGCTCCCA GCCCAGAGGC CCTCCCCTCC CCAGGCCAGG AGCCCGCAGC TGGGGCTAGC 
CCCAGGCGGG GAGAGCTCCG CAGGGGCAGC TCGGCTGAGA GCGCCCTGCC CCGGGCCGGG 
CCGCGGGAGC TGGGCCGGGG CCTGCACAAG GCGGCGTCTG TGGAGCTGCC GCAGCGCCGG 
AGCCCCAGCC CGGGAGCCAC CCGCCTGGCC CGGGGAGGCC TGGGTGAGGG CGAGTATGCC 
CAGAGGCTGC AGGCCCTGCG CCAGCGGCTG CTGCGGGGAG GCCCCGAGGA TGGCAAGGTC 
AGCGGCCTCA GGGGTCCCCT GCTGGAGAGC CTGGGGGGCC GTGCTCGGGA CCCCCGGATG 
GCACGAGCTG CCTCCAGCGA GGCAGCGCCC CACCACCAGC CCCCACTCGA GAACCGGGGC 
CTGCAAAAGA GCAGCAGCTT CTCCCAGGGT GAGGCGGAGC CCCGGGGCCG GCACCGCCGA 
GCGGGGGCGC CCCTCGAGAT CCCCGTGGCC AGGCTTGGGG CCCGTAGGCT ACAGGAGTCT 
CCTTCCCTGT CTGCCCTCAG CGAGGCCCAG CCATCCAGCC CTGCACGGCC CAGCGCCCCC 
AAACCCAGTA CCCCTAAGTC TGCAGAACCT TCTGCCACCA CACCTAGTGA TGCTCCGCAG 
CCCCCCGCAC CCCAGCCTGC CCAAGACAAG GCTCCAGAGC CCAGGCCAGA ACCAGTCCGA 
GCCTCCAAGC CTGCACCACC CCCCCAGGCC CTGCAAACCC TAGCGCTGCC CCTCACACCC 
TATGCTCAGA TCATTCAGTC CCTCCAGCTG TCAGGCCACG CCCAGGGCCC CTCGCAGGGC 
CCTGCCGCGC CGCCTTCAGA GCCCAAGCCC CACGCTGCTG TCTTTGCCAG GGTGGCCTCC 
CCACCTCCGG GAGCCCCCGA GAAGCGCGTG CCCTCAGCCG GGGGTCCCCC GGTGCTAGCC 
GAGAAAGCCC GAGTTCCCAC GGTGCCCCCC AGGCCAGGCA GCAGTCTCAG TAGCAGCATC 
GAAAACTTGG AGTCGGAGGC CGTGTTCGAG GCCAAGTTCA AGCGCAGCCG CGAGTCGCCC 
CTGTCGCTGG GGCTGCGGCT GCTGAGCCGT TCGCGCTCGG AGGAGCGCGG CCCCTTCCGT 
GGGGCCGAGG AGGAGGATGG CATATACCGG CCCAGCCCGG CGGGGACCCC GCTGGAGCTG 
GTGCGACGGC CTGAGCGCTC ACGCTCGGTG CAGGACCTCA GGGCTGTCGG AGAGCCTGGC 
CTCGTCCGCC GCCTCTCGCT GTCACTGTCC CAGCGGCTGC GGCGGACCCC TCCCGCGCAG 
CGCCACCCGG CCTGGGAGGC CCGCGGCGGG GACGGAGAGA GCTCGGAGGG CGGGAGCTCG 
GCGCGGGGCT CCCCGGTGCT GGCGATGCGC AGGCGGCTGA GCTTCACCCT GGAGCGGCTG 
TCCAGCCGAT TGCAGCGCAG TGGCAGCAGC GAGGACTCGG GGGGCGCGTC GGGCCGCAGC 
ACGCCGCTGT TCGGACGGCT TCGCAGGGCC ACGTCCGAGG GCGAGAGTCT GCGGCGCCTT 
GGCCTTCCGC ACAACCAGTT GGCCGCCCAG GCCGGCGCCA CCACGCCTTC CGCCGAGTCC 
CTGGGCTCCG AGGCCAGCGC CACGTCGGGC TCCTCAGCCC CAGGGGAAAG CCGAAGCCGG 
CTCCGCTGGG GCTTCTCTCG GCCGCGGAAG GACAAGGGGT TATCGCCACC AAACCTCTCT 
GCCAGCGTCC AGGAGGAGTT GGGTCACCAG TACGTGCGCA GTGAGTCAGA CTTCCCCCCA 
GTCTTCCACA TCAAACTCAA GGACCAGGTG CTGCTGGAGG GGGAGGCAGC CACCCTGCTC 
TGCCTGCCAG CGGCCTGCCC TGCACCGCAC ATCTCCTGGA TGAAAGACAA GAAGTCCTTG 
AGGTCAGAGC CCTCAGTGAT CATCGTGTCC TGCAAAGATG GGCGGCAGCT GCTCAGCATC 
CCCCGGGCGG GCAAGCGGCA CGCCGGTCTC TATGAGTGCT CGGCCACCAA CGTACTGGGC 
AGCATCACCA GCTCCTGTAC CGTGGCTGTG GCCCGAGTCC CAGGAAAGCT AGCTCCTCCA 
GAGGTACCCC AGACCTACCA GGACACGGCG CTGGTGCTGT GGAAGCCGGG AGACAGCCGG 
GCACCTTGCA CGTATACGCT GGAGCGGCGA GTGGATGGGG AGTCTGTGTG GCACCCTGTG 
AGCTCAGGCA TCCCCGACTG TTACTACAAC GTGACCCACC TGCCAGTTGG CGTGACTGTG 
AGGTTCCGTG TGGCCTGTGC CAACCGTGCT GGGCAGGGGC CCTTCAGCAA CTCTTCTGAG 
AAGGTCTTTG TCAGGGGTAC TCAAGATTCT TCAGCTGTGC CATCTGCTGC CCACCAAGAG 
GCCCCTGTCA CCTCAAGGCC AGCCAGGGCC CGGCCTCCTG ACTCTCCTAC CTCACTGGCC 
CCACCCCTAG CTCCTGCTGC CCCCACACCC CCGTCAGTCA CTGTCAGCCC CTCATCTCCC 
CCCACACCTC CTAGCCAGGC CTTGTCCTCG CTCAAGGCTG TGGGTCCACC ACCCCAAACC 
CCTCCACGAA GACACAGGGG CCTGCAGGCT GCCCGGCCAG CGGAGCCCAC CCTACCCAGT 
ACCCACGTCA CCCCAAGTGA GCCCAAGCCT TTCGTCCTTG ACACTGGGAC CCCGATCCCA 
GCCTCCACTC CTCAAGGGGT TAAACCAGTG TCTTCCTCTA CTCCTGTGTA TGTGGTGACT 
TCCTTTGTGT CTGCACCACC AGCCCCTGAG CCCCCAGCCC CTGAGCCCCC TCCTGAGCCT 
ACCAAGGTGA CTGTGCAGAG CCTCAGCCCG GCCAAGGAGG TGGTCAGCTC CCCTGGGAGC 
AGTCCCCGAA GCTCTCCCAG GCCTGAGGGT ACCACTCTTC GACAGGGTCC CCCTCAGAAA 
CCCTACACCT TCCTGGAGGA GAAAGCCAGG GGCCGCTTTG GTGTTGTGCG AGCGTGCCGG 
GAGAATGCCA CGGGGCGAAC GTTCGTGGCC AAGATCGTGC CCTATGCTGC CGAGGGCAAG 
CGGCGGGTCC TGCAGGAGTA CGAGGTGCTG CGGACCCTGC ACCACGAGCG GATCATGTCC 
CTGCACGAGG CCTACATCAC CCCTCGGTAC CTCGTGCTCA TTGCTGAGAG CTGTGGCAAC 
CGGGAACTCC TCTGTGGGCT CAGTGACAGG TTCCGGTATT CTGAGGATGA CGTGGCCACT 
TACATGGTGC AGCTGCTACA AGGCCTGGAC TACCTCCACG GCCACCACGT GCTCCACCTA 
GACATCAAGC CAGACAACCT GCTGCTGGCC CCTGACAATG CCCTCAAGAT TGTGGACTTT 
GGCAGTGCCC AGCCCTACAA CCCCCAGGCC CTTAGGCCCC TTGGCCACCG CACGGGCACG 
CTGGAGTTCA TGGCTCCGGA GATGGTGAAG GGAGAACCCA TCGGCTCTGC CACGGACATC 
TGGGGAGCGG GTGTGCTCAC TTACATTATG CTCAGTGGAC GCTCCCCGTT CTATGAGCCA 
GACCCCCAGG AAACGGAGGC TCGGATTGTG GGGGGCCGCT TTGATGCCTT CCAGCTGTAC 
CCCAATACAT CCCAGAGCGC CACCCTCTTC TTGCGAAAGG TTCTCTCTGT ACATCCCTGG 
AGCCGGCCCT CCCTGCAGGA CTGCCTGGCC CACCCATGGT TGCAGGACGC CTACCTGATG 
AAGCTGCGCC GCCAGACGCT CACCTTCACC ACCAACCGGC TCAAGGAGTT CCTGGGCGAG 
CAGCGGCGGC GCCGGGCTGA GGCTGCCACC CGCCACAAGG TGCTGCTGCG CTCCTACCCT 
GGCGGCCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712252.1
CDS	46..9537
Translation	MKKLWVKKRFQKTGHSRRAFGRLTHDSETAEDDISDVQGTQRLELRDDGAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRRLPGSPRQAQATGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSVPQSGLRREEPDLQPQLASEAPRRPAQPPPSKSALLPPPSPRVGKRSPPGPPAQPAATPTSPHRRTQEPVLPEDTTTEEKRGKKSKSSGPSLAGTAESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSERGAPWGTPGASQEELRAPGSVAERRRLFQQKAASLDERTRQRSPASDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAAAQPPSPSSAEKPGDEPGRPRSRGPAGRTEPGEGPQQEVRRRDQFPLTRSRAIQECRSPVPPPAADPPEARTKAPPGRKREPPAQAVRFLPWATPGLEGAAVPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGARSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNVVVAPGADVLLKCIITANPPPQVSWHKDGSALRSEGRLLLRAEGERHTLLLREARAADAGSYMATATNELGQATCAASLTVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPTDVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARFDCKISGTPPPVVTWTHFGCPMEESENLRLRQDGGLHSLHIAHVGSEDEGLYAVSAVNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEQGELERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDVHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPQVVAVTGRMVTLTWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPGWAATGLRKGVQHIFRVLSTTVKSSSKPSPPSEPVQLLEHGPTLEEAPAMLDKPDIVYVVEGQPASVTVTFNHVEAQVVWRSCRGALLEARAGVYELSQPDDDQYCLRICRVSRRDMGALTCTARNRHGTQTCSVTLELAEAPRFESIMEDVEVGAGETARFAVVVEGKPLPDIMWYKDEVLLTESSHVSFVYEENECSLVVLSTGAQDGGVYTCTAQNLAGEVSCKAELAVHSAQTAMEVEGVGEDEDHRGRRLSDFYDIHQEIGRGAFSYLRRIVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHDCVLYFHEAFERRRGLVIVTELCTEELLERIARKPTVCESEIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTQAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGELRRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQPPAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPSEPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEAKFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAVGEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAESLGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPHISWMKDKKSLRSEPSVIIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARVPGKLAPPEVPQTYQDTALVLWKPGDSRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTHLPVGVTVRFRVACANRAGQGPFSNSSEKVFVRGTQDSSAVPSAAHQEAPVTSRPARARPPDSPTSLAPPLAPAAPTPPSVTVSPSSPPTPPSQALSSLKAVGPPPQTPPRRHRGLQAARPAEPTLPSTHVTPSEPKPFVLDTGTPIPASTPQGVKPVSSSTPVYVVTSFVSAPPAPEPPAPEPPPEPTKVTVQSLSPAKEVVSSPGSSPRSSPRPEGTTLRQGPPQKPYTFLEEKARGRFGVVRACRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERIMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYMVQLLQGLDYLHGHHVLHLDIKPDNLLLAPDNALKIVDFGSAQPYNPQALRPLGHRTGTLEFMAPEMVKGEPIGSATDIWGAGVLTYIMLSGRSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGGP

Target ORF information:

RefSeq Version	XM_006712189.3
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712189.3

ORF Insert Sequence:

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTCA CCCATGACTC AGAGACGGCT GAGGATGACA TCAGCGATGT GCAGGGAACC 
CAGCGCCTGG AGCTTCGGGA TGACGGGGCC TTCAGCACCC CCACGGGGGG TTCTGACACC 
CTGGTGGGCA CCTCCCTGGA CACACCCCCG ACCTCCGTGA CAGGCACCTC AGAGGAGCAA 
GTGAGCTGGT GGGGCAGCGG GCAGACGGTC CTGGAGCAGG AAGCGGGCAG TGGGGGTGGC 
ACCCGCCGCC TCCCGGGCAG CCCAAGGCAA GCACAGGCAA CCGGGGCCGG GCCACGGCAC 
CTGGGGGTGG AGCCGCTGGT GCGGGCATCT CGAGCTAATC TGGTGGGCGC AAGCTGGGGG 
TCAGAGGATA GCCTTTCCGT GGCCAGTGAC CTGTACGGCA GCGCATTCAG CCTGTACAGA 
GGACGGGCGC TCTCTATCCA CGTCAGCGTC CCTCAGAGCG GGTTGCGCAG GGAGGAGCCC 
GACCTTCAGC CTCAACTGGC CAGCGAAGCC CCACGCCGCC CTGCCCAGCC GCCTCCTTCC 
AAATCCGCGC TGCTCCCCCC ACCGTCCCCT CGGGTCGGGA AGCGGTCCCC GCCGGGACCC 
CCGGCCCAGC CCGCGGCCAC CCCCACGTCG CCCCACCGTC GCACTCAGGA GCCTGTGCTG 
CCCGAGGACA CCACCACCGA AGAGAAGCGA GGGAAGAAGT CCAAGTCGTC CGGGCCCTCC 
CTGGCGGGCA CCGCGGAATC CCGACCCCAG ACGCCACTGA GCGAGGCCTC AGGCCGCCTG 
TCGGCGTTGG GCCGATCGCC TAGGCTGGTG CGCGCCGGCT CCCGCATCCT GGACAAGCTG 
CAGTTCTTCG AGGAGCGACG GCGCAGCCTG GAGCGCAGCG ACTCGCCGCC GGCGCCCCTG 
CGGCCCTGGG TGCCCCTGCG CAAGGCCCGC TCTCTGGAGC AGCCCAAGTC GGAGCGCGGC 
GCACCGTGGG GCACCCCCGG GGCCTCGCAG GAAGAACTGC GGGCGCCAGG CAGCGTGGCC 
GAGCGGCGCC GCCTGTTCCA GCAGAAAGCG GCCTCGCTGG ACGAGCGCAC GCGTCAGCGC 
AGCCCGGCCT CAGACCTCGA GCTGCGCTTC GCCCAGGAGC TGGGCCGCAT CCGCCGCTCC 
ACGTCGCGGG AGGAGCTGGT GCGCTCGCAC GAGTCCCTGC GCGCCACGCT GCAGCGTGCC 
CCATCCCCTC GAGAGCCCGG CGAGCCCCCG CTCTTCTCTC GGCCCTCCAC CCCCAAGACA 
TCGCGGGCCG TGAGCCCCGC CGCCGCCCAG CCGCCCTCTC CGAGCAGCGC GGAGAAGCCG 
GGGGACGAGC CTGGGAGGCC CAGGAGCCGC GGGCCGGCGG GCAGGACAGA GCCGGGGGAA 
GGCCCGCAGC AGGAGGTTAG GCGTCGGGAC CAATTCCCGC TGACCCGGAG CAGAGCCATC 
CAGGAGTGCA GGAGCCCTGT GCCGCCCCCC GCCGCCGATC CCCCAGAGGC CAGGACGAAA 
GCACCCCCCG GTCGGAAGCG GGAGCCCCCG GCGCAGGCCG TGCGCTTCCT GCCCTGGGCC 
ACGCCGGGCC TGGAGGGCGC TGCTGTACCC CAGACCTTGG AGAAGAACAG GGCGGGGCCT 
GAGGCAGAGA AGAGGCTTCG CAGAGGGCCG GAGGAGGACG GTCCCTGGGG GCCCTGGGAC 
CGCCGAGGGG CCCGCAGCCA GGGCAAAGGT CGCCGGGCCC GGCCCACCTC CCCTGAGCTC 
GAGTCTTCGG ATGACTCCTA CGTGTCCGCT GGAGAAGAGC CCCTAGAGGC CCCTGTGTTT 
GAGATCCCCC TGCAGAATGT GGTGGTGGCA CCAGGGGCAG ATGTGCTGCT CAAGTGTATC 
ATCACTGCCA ACCCCCCGCC CCAAGTGTCC TGGCACAAGG ATGGGTCAGC GCTGCGCAGC 
GAGGGCCGCC TCCTCCTCCG GGCTGAGGGT GAGCGGCACA CCCTGCTGCT CAGGGAGGCC 
AGGGCAGCAG ATGCCGGGAG CTATATGGCC ACCGCCACCA ACGAGCTGGG CCAGGCCACC 
TGTGCCGCCT CACTGACCGT GAGACCCGGT GGGTCTACAT CCCCTTTCAG CAGCCCCATC 
ACCTCCGACG AGGAATACCT GAGCCCCCCA GAGGAGTTCC CAGAGCCTGG GGAGACCTGG 
CCGCGAACCC CCACCATGAA GCCCAGTCCC AGCCAGAACC GCCGTTCTTC TGACACTGGC 
TCCAAGGCAC CCCCCACCTT CAAGGTCTCA CTTATGGACC AGTCAGTAAG AGAAGGCCAA 
GATGTCATCA TGAGCATCCG CGTGCAGGGG GAGCCCAAGC CTGTGGTCTC CTGGCTGAGA 
AACCGCCAGC CCGTGCGCCC AGACCAGCGG CGCTTTGCGG AGGAGGCTGA GGGTGGGCTG 
TGCCGGCTGC GGATCCTGGC TGCAGAGCGT GGCGATGCTG GTTTCTACAC TTGCAAAGCG 
GTCAATGAGT ATGGTGCTCG GCAGTGCGAG GCCCGCTTGG AGGTCCGAGC ACACCCTGAA 
AGCCGGTCCC TGGCCGTGCT GGCCCCCCTG CAGGACGTGG ACGTGGGGGC CGGGGAGATG 
GCGCTGTTTG AGTGCCTGGT GGCGGGGCCC ACTGACGTGG AGGTGGATTG GCTGTGCCGT 
GGCCGCCTGC TGCAGCCTGC ACTGCTCAAA TGCAAGATGC ATTTCGATGG CCGCAAATGC 
AAGCTGCTAC TTACATCTGT ACATGAGGAC GACAGTGGCG TCTACACCTG CAAGCTCAGC 
ACGGCCAAAG ATGAGCTGAC CTGCAGTGCC CGGCTGACCG TGCGGCCCTC GTTGGCACCC 
CTGTTCACAC GGCTGCTGGA AGATGTGGAG GTGTTGGAGG GCCGAGCTGC CCGTTTCGAC 
TGCAAGATCA GTGGCACCCC GCCCCCTGTT GTTACCTGGA CTCATTTTGG CTGCCCCATG 
GAGGAGAGTG AGAACTTGCG GCTGCGGCAG GACGGGGGTC TGCACTCACT GCACATTGCC 
CATGTGGGCA GCGAGGACGA GGGGCTCTAT GCGGTCAGTG CTGTTAACAC CCATGGCCAG 
GCCCACTGCT CAGCCCAGCT GTATGTAGAA GAGCCCCGGA CAGCCGCCTC AGGCCCCAGC 
TCGAAGCTGG AGAAGATGCC ATCCATTCCC GAGGAGCCAG AGCAGGGTGA GCTGGAGCGG 
CTGTCCATTC CTGACTTCCT GCGGCCACTG CAGGACCTGG AGGTGGGACT GGCCAAGGAG 
GCCATGCTAG AGTGCCAGGT GACCGGCCTG CCCTACCCCA CCATCAGCTG GTTCCACAAT 
GGCCACCGCA TCCAGAGCAG CGACGACCGG CGCATGACAC AGTACAGGGA TGTCCATCGC 
TTGGTGTTCC CTGCCGTGGG GCCTCAGCAC GCCGGTGTCT ACAAGAGCGT CATTGCCAAC 
AAGCTGGGCA AAGCTGCCTG CTATGCCCAC CTGTATGTCA CAGATGTGGT CCCAGGCCCT 
CCAGATGGCG CCCCGCAGGT GGTGGCTGTG ACGGGGAGGA TGGTCACACT CACATGGAAC 
CCCCCCAGGA GTCTGGACAT GGCCATCGAC CCGGACTCCC TGACGTACAC AGTGCAGCAC 
CAGGTGCTGG GCTCGGACCA GTGGACGGCA CTGGTCACAG GCCTGCGGGA GCCAGGGTGG 
GCAGCCACAG GGCTGCGTAA GGGGGTCCAG CACATCTTCC GGGTCCTCAG CACCACTGTC 
AAGAGCAGCA GCAAGCCCTC ACCCCCTTCT GAGCCTGTGC AGCTGCTGGA GCACGGCCCA 
ACCCTGGAGG AGGCCCCTGC CATGCTGGAC AAACCAGACA TCGTGTATGT GGTGGAGGGA 
CAGCCTGCCA GCGTCACCGT CACATTCAAC CATGTGGAGG CCCAGGTCGT CTGGAGGAGC 
TGCCGAGGGG CCCTCCTAGA GGCACGGGCC GGTGTGTACG AGCTGAGCCA GCCAGATGAT 
GACCAGTACT GTCTTCGGAT CTGCCGGGTG AGCCGCCGGG ACATGGGGGC CCTCACCTGC 
ACCGCCCGAA ACCGTCACGG CACACAGACC TGCTCGGTCA CATTGGAGCT GGCAGAGGCC 
CCTCGGTTTG AGTCCATCAT GGAGGACGTG GAGGTGGGGG CTGGGGAAAC TGCTCGCTTT 
GCGGTGGTGG TCGAGGGAAA ACCACTGCCG GACATCATGT GGTACAAGGA CGAGGTGCTG 
CTGACCGAGA GCAGCCATGT GAGCTTCGTG TACGAGGAGA ATGAGTGCTC CCTGGTGGTG 
CTCAGCACGG GGGCCCAGGA TGGAGGCGTC TACACCTGCA CCGCCCAGAA CCTGGCGGGT 
GAGGTCTCCT GCAAAGCAGA GTTGGCTGTG CATTCAGCTC AGACAGCTAT GGAGGTCGAG 
GGGGTCGGGG AGGATGAGGA CCATCGAGGA AGGAGACTCA GCGACTTTTA TGACATCCAC 
CAGGAGATCG GCAGGGGTGC TTTCTCCTAC TTGCGGCGCA TAGTGGAGCG TAGCTCCGGC 
CTGGAGTTTG CGGCCAAGTT CATCCCCAGC CAGGCCAAGC CAAAGGCATC AGCGCGTCGG 
GAGGCCCGGC TGCTGGCCAG GCTCCAGCAC GACTGTGTCC TCTACTTCCA TGAGGCCTTC 
GAGAGGCGCC GGGGACTGGT CATTGTCACC GAGCTCTGCA CAGAGGAGCT GCTGGAGCGA 
ATCGCCAGGA AACCCACCGT GTGTGAGTCT GAGATCCGGG CCTATATGCG GCAGGTGCTA 
GAGGGAATAC ACTACCTGCA CCAGAGCCAC GTGCTGCACC TCGATGTCAA GCCTGAGAAC 
CTGCTGGTGT GGGATGGTGC TGCGGGCGAG CAGCAGGTGC GGATCTGTGA CTTTGGGAAT 
GCCCAGGAGC TGACTCCAGG AGAGCCCCAG TACTGCCAGT ATGGCACACC TGAGTTTGTA 
GCACCCGAGA TTGTCAATCA GAGCCCCGTG TCTGGAGTCA CTGACATCTG GCCTGTGGGT 
GTTGTTGCCT TCCTCTGTCT GACAGGAATC TCCCCGTTTG TTGGGGAAAA TGACCGGACA 
ACATTGATGA ACATCCGAAA CTACAACGTG GCCTTCGAGG AGACCACATT CCTGAGCCTG 
AGCAGGGAGG CCCGGGGCTT CCTCATCAAA GTGTTGGTGC AGGACCGGCT GAGACCTACC 
GCAGAAGAGA CCCTAGAACA TCCTTGGTTC AAAACTCAGG CAAAGGGCGC AGAGGTGAGC 
ACGGATCACC TGAAGCTATT CCTCTCCCGG CGGAGGTGGC AGCGCTCCCA GATCAGCTAC 
AAATGCCACC TGGTGCTGCG CCCCATCCCC GAGCTGCTGC GGGCCCCCCC AGAGCGGGTG 
TGGGTGACCA TGCCCAGAAG GCCACCCCCC AGTGGGGGGC TCTCATCCTC CTCGGATTCT 
GAAGAGGAAG AGCTGGAAGA GCTGCCCTCA GTGCCCCGCC CACTGCAGCC CGAGTTCTCT 
GGCTCCCGGG TGTCCCTCAC AGACATTCCC ACTGAGGATG AGGCCCTGGG GACCCCAGAG 
ACTGGGGCTG CCACCCCCAT GGACTGGCAG GAGCAGGGAA GGGCTCCCTC TCAGGACCAG 
GAGGCTCCCA GCCCAGAGGC CCTCCCCTCC CCAGGCCAGG AGCCCGCAGC TGGGGCTAGC 
CCCAGGCGGG GAGAGCTCCG CAGGGGCAGC TCGGCTGAGA GCGCCCTGCC CCGGGCCGGG 
CCGCGGGAGC TGGGCCGGGG CCTGCACAAG GCGGCGTCTG TGGAGCTGCC GCAGCGCCGG 
AGCCCCAGCC CGGGAGCCAC CCGCCTGGCC CGGGGAGGCC TGGGTGAGGG CGAGTATGCC 
CAGAGGCTGC AGGCCCTGCG CCAGCGGCTG CTGCGGGGAG GCCCCGAGGA TGGCAAGGTC 
AGCGGCCTCA GGGGTCCCCT GCTGGAGAGC CTGGGGGGCC GTGCTCGGGA CCCCCGGATG 
GCACGAGCTG CCTCCAGCGA GGCAGCGCCC CACCACCAGC CCCCACTCGA GAACCGGGGC 
CTGCAAAAGA GCAGCAGCTT CTCCCAGGGT GAGGCGGAGC CCCGGGGCCG GCACCGCCGA 
GCGGGGGCGC CCCTCGAGAT CCCCGTGGCC AGGCTTGGGG CCCGTAGGCT ACAGGAGTCT 
CCTTCCCTGT CTGCCCTCAG CGAGGCCCAG CCATCCAGCC CTGCACGGCC CAGCGCCCCC 
AAACCCAGTA CCCCTAAGTC TGCAGAACCT TCTGCCACCA CACCTAGTGA TGCTCCGCAG 
CCCCCCGCAC CCCAGCCTGC CCAAGACAAG GCTCCAGAGC CCAGGCCAGA ACCAGTCCGA 
GCCTCCAAGC CTGCACCACC CCCCCAGGCC CTGCAAACCC TAGCGCTGCC CCTCACACCC 
TATGCTCAGA TCATTCAGTC CCTCCAGCTG TCAGGCCACG CCCAGGGCCC CTCGCAGGGC 
CCTGCCGCGC CGCCTTCAGA GCCCAAGCCC CACGCTGCTG TCTTTGCCAG GGTGGCCTCC 
CCACCTCCGG GAGCCCCCGA GAAGCGCGTG CCCTCAGCCG GGGGTCCCCC GGTGCTAGCC 
GAGAAAGCCC GAGTTCCCAC GGTGCCCCCC AGGCCAGGCA GCAGTCTCAG TAGCAGCATC 
GAAAACTTGG AGTCGGAGGC CGTGTTCGAG GCCAAGTTCA AGCGCAGCCG CGAGTCGCCC 
CTGTCGCTGG GGCTGCGGCT GCTGAGCCGT TCGCGCTCGG AGGAGCGCGG CCCCTTCCGT 
GGGGCCGAGG AGGAGGATGG CATATACCGG CCCAGCCCGG CGGGGACCCC GCTGGAGCTG 
GTGCGACGGC CTGAGCGCTC ACGCTCGGTG CAGGACCTCA GGGCTGTCGG AGAGCCTGGC 
CTCGTCCGCC GCCTCTCGCT GTCACTGTCC CAGCGGCTGC GGCGGACCCC TCCCGCGCAG 
CGCCACCCGG CCTGGGAGGC CCGCGGCGGG GACGGAGAGA GCTCGGAGGG CGGGAGCTCG 
GCGCGGGGCT CCCCGGTGCT GGCGATGCGC AGGCGGCTGA GCTTCACCCT GGAGCGGCTG 
TCCAGCCGAT TGCAGCGCAG TGGCAGCAGC GAGGACTCGG GGGGCGCGTC GGGCCGCAGC 
ACGCCGCTGT TCGGACGGCT TCGCAGGGCC ACGTCCGAGG GCGAGAGTCT GCGGCGCCTT 
GGCCTTCCGC ACAACCAGTT GGCCGCCCAG GCCGGCGCCA CCACGCCTTC CGCCGAGTCC 
CTGGGCTCCG AGGCCAGCGC CACGTCGGGC TCCTCAGCCC CAGGGGAAAG CCGAAGCCGG 
CTCCGCTGGG GCTTCTCTCG GCCGCGGAAG GACAAGGGGT TATCGCCACC AAACCTCTCT 
GCCAGCGTCC AGGAGGAGTT GGGTCACCAG TACGTGCGCA GTGAGTCAGA CTTCCCCCCA 
GTCTTCCACA TCAAACTCAA GGACCAGGTG CTGCTGGAGG GGGAGGCAGC CACCCTGCTC 
TGCCTGCCAG CGGCCTGCCC TGCACCGCAC ATCTCCTGGA TGAAAGACAA GAAGTCCTTG 
AGGTCAGAGC CCTCAGTGAT CATCGTGTCC TGCAAAGATG GGCGGCAGCT GCTCAGCATC 
CCCCGGGCGG GCAAGCGGCA CGCCGGTCTC TATGAGTGCT CGGCCACCAA CGTACTGGGC 
AGCATCACCA GCTCCTGTAC CGTGGCTGTG GCCCGAGTCC CAGGAAAGCT AGCTCCTCCA 
GAGGTACCCC AGACCTACCA GGACACGGCG CTGGTGCTGT GGAAGCCGGG AGACAGCCGG 
GCACCTTGCA CGTATACGCT GGAGCGGCGA GTGGATGGGG AGTCTGTGTG GCACCCTGTG 
AGCTCAGGCA TCCCCGACTG TTACTACAAC GTGACCCACC TGCCAGTTGG CGTGACTGTG 
AGGTTCCGTG TGGCCTGTGC CAACCGTGCT GGGCAGGGGC CCTTCAGCAA CTCTTCTGAG 
AAGGTCTTTG TCAGGGGTAC TCAAGATTCT TCAGCTGTGC CATCTGCTGC CCACCAAGAG 
GCCCCTGTCA CCTCAAGGCC AGCCAGGGCC CGGCCTCCTG ACTCTCCTAC CTCACTGGCC 
CCACCCCTAG CTCCTGCTGC CCCCACACCC CCGTCAGTCA CTGTCAGCCC CTCATCTCCC 
CCCACACCTC CTAGCCAGGC CTTGTCCTCG CTCAAGGCTG TGGGTCCACC ACCCCAAACC 
CCTCCACGAA GACACAGGGG CCTGCAGGCT GCCCGGCCAG CGGAGCCCAC CCTACCCAGT 
ACCCACGTCA CCCCAAGTGA GCCCAAGCCT TTCGTCCTTG ACACTGGGAC CCCGATCCCA 
GCCTCCACTC CTCAAGGGGT TAAACCAGTG TCTTCCTCTA CTCCTGTGTA TGTGGTGACT 
TCCTTTGTGT CTGCACCACC AGCCCCTGAG CCCCCAGCCC CTGAGCCCCC TCCTGAGCCT 
ACCAAGGTGA CTGTGCAGAG CCTCAGCCCG GCCAAGGAGG TGGTCAGCTC CCCTGGGAGC 
AGTCCCCGAA GCTCTCCCAG GCCTGAGGGT ACCACTCTTC GACAGGGTCC CCCTCAGAAA 
CCCTACACCT TCCTGGAGGA GAAAGCCAGG GGCCGCTTTG GTGTTGTGCG AGCGTGCCGG 
GAGAATGCCA CGGGGCGAAC GTTCGTGGCC AAGATCGTGC CCTATGCTGC CGAGGGCAAG 
CGGCGGGTCC TGCAGGAGTA CGAGGTGCTG CGGACCCTGC ACCACGAGCG GATCATGTCC 
CTGCACGAGG CCTACATCAC CCCTCGGTAC CTCGTGCTCA TTGCTGAGAG CTGTGGCAAC 
CGGGAACTCC TCTGTGGGCT CAGTGACAGG TTCCGGTATT CTGAGGATGA CGTGGCCACT 
TACATGGTGC AGCTGCTACA AGGCCTGGAC TACCTCCACG GCCACCACGT GCTCCACCTA 
GACATCAAGC CAGACAACCT GCTGCTGGCC CCTGACAATG CCCTCAAGAT TGTGGACTTT 
GGCAGTGCCC AGCCCTACAA CCCCCAGGCC CTTAGGCCCC TTGGCCACCG CACGGGCACG 
CTGGAGTTCA TGGCTCCGGA GATGGTGAAG GGAGAACCCA TCGGCTCTGC CACGGACATC 
TGGGGAGCGG GTGTGCTCAC TTACATTATG CTCAGTGGAC GCTCCCCGTT CTATGAGCCA 
GACCCCCAGG AAACGGAGGC TCGGATTGTG GGGGGCCGCT TTGATGCCTT CCAGCTGTAC 
CCCAATACAT CCCAGAGCGC CACCCTCTTC TTGCGAAAGG TTCTCTCTGT ACATCCCTGG 
AGCCGGCCCT CCCTGCAGGA CTGCCTGGCC CACCCATGGT TGCAGGACGC CTACCTGATG 
AAGCTGCGCC GCCAGACGCT CACCTTCACC ACCAACCGGC TCAAGGAGTT CCTGGGCGAG 
CAGCGGCGGC GCCGGGCTGA GGCTGCCACC CGCCACAAGG TGCTGCTGCG CTCCTACCCT 
GGCGGCCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_005246239.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005246296.1 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X7, mRNA.

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CloneID	OHu35755
Clone ID Related Accession (Same CDS sequence)	XM_005246239.2
Accession Version	XM_005246239.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005246239.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCG CCACCAACGA GCTGGGCCAG GCCACCTGTG CCGCCTCACT GACCGTGAGA 
CCCGGTGGGT CTACATCCCC TTTCAGCAGC CCCATCACCT CCGACGAGGA ATACCTGAGC 
CCCCCAGAGG AGTTCCCAGA GCCTGGGGAG ACCTGGCCGC GAACCCCCAC CATGAAGCCC 
AGTCCCAGCC AGAACCGCCG TTCTTCTGAC ACTGGCTCCA AGGCACCCCC CACCTTCAAG 
GTCTCACTTA TGGACCAGTC AGTAAGAGAA GGCCAAGATG TCATCATGAG CATCCGCGTG 
CAGGGGGAGC CCAAGCCTGT GGTCTCCTGG CTGAGAAACC GCCAGCCCGT GCGCCCAGAC 
CAGCGGCGCT TTGCGGAGGA GGCTGAGGGT GGGCTGTGCC GGCTGCGGAT CCTGGCTGCA 
GAGCGTGGCG ATGCTGGTTT CTACACTTGC AAAGCGGTCA ATGAGTATGG TGCTCGGCAG 
TGCGAGGCCC GCTTGGAGGT CCGAGCACAC CCTGAAAGCC GGTCCCTGGC CGTGCTGGCC 
CCCCTGCAGG ACGTGGACGT GGGGGCCGGG GAGATGGCGC TGTTTGAGTG CCTGGTGGCG 
GGGCCCACTG ACGTGGAGGT GGATTGGCTG TGCCGTGGCC GCCTGCTGCA GCCTGCACTG 
CTCAAATGCA AGATGCATTT CGATGGCCGC AAATGCAAGC TGCTACTTAC ATCTGTACAT 
GAGGACGACA GTGGCGTCTA CACCTGCAAG CTCAGCACGG CCAAAGATGA GCTGACCTGC 
AGTGCCCGGC TGACCGTGCG GCCCTCGTTG GCACCCCTGT TCACACGGCT GCTGGAAGAT 
GTGGAGGTGT TGGAGGGCCG AGCTGCCCGT TTCGACTGCA AGATCAGTGG CACCCCGCCC 
CCTGTTGTTA CCTGGACTCA TTTTGGCTGC CCCATGGAGG AGAGTGAGAA CTTGCGGCTG 
CGGCAGGACG GGGGTCTGCA CTCACTGCAC ATTGCCCATG TGGGCAGCGA GGACGAGGGG 
CTCTATGCGG TCAGTGCTGT TAACACCCAT GGCCAGGCCC ACTGCTCAGC CCAGCTGTAT 
GTAGAAGAGC CCCGGACAGC CGCCTCAGGC CCCAGCTCGA AGCTGGAGAA GATGCCATCC 
ATTCCCGAGG AGCCAGAGCA GGGTGAGCTG GAGCGGCTGT CCATTCCTGA CTTCCTGCGG 
CCACTGCAGG ACCTGGAGGT GGGACTGGCC AAGGAGGCCA TGCTAGAGTG CCAGGTGACC 
GGCCTGCCCT ACCCCACCAT CAGCTGGTTC CACAATGGCC ACCGCATCCA GAGCAGCGAC 
GACCGGCGCA TGACACAGTA CAGGGATGTC CATCGCTTGG TGTTCCCTGC CGTGGGGCCT 
CAGCACGCCG GTGTCTACAA GAGCGTCATT GCCAACAAGC TGGGCAAAGC TGCCTGCTAT 
GCCCACCTGT ATGTCACAGA TGTGGTCCCA GGCCCTCCAG ATGGCGCCCC GCAGGTGGTG 
GCTGTGACGG GGAGGATGGT CACACTCACA TGGAACCCCC CCAGGAGTCT GGACATGGCC 
ATCGACCCGG ACTCCCTGAC GTACACAGTG CAGCACCAGG TGCTGGGCTC GGACCAGTGG 
ACGGCACTGG TCACAGGCCT GCGGGAGCCA GGGTGGGCAG CCACAGGGCT GCGTAAGGGG 
GTCCAGCACA TCTTCCGGGT CCTCAGCACC ACTGTCAAGA GCAGCAGCAA GCCCTCACCC 
CCTTCTGAGC CTGTGCAGCT GCTGGAGCAC GGCCCAACCC TGGAGGAGGC CCCTGCCATG 
CTGGACAAAC CAGACATCGT GTATGTGGTG GAGGGACAGC CTGCCAGCGT CACCGTCACA 
TTCAACCATG TGGAGGCCCA GGTCGTCTGG AGGAGCTGCC GAGGGGCCCT CCTAGAGGCA 
CGGGCCGGTG TGTACGAGCT GAGCCAGCCA GATGATGACC AGTACTGTCT TCGGATCTGC 
CGGGTGAGCC GCCGGGACAT GGGGGCCCTC ACCTGCACCG CCCGAAACCG TCACGGCACA 
CAGACCTGCT CGGTCACATT GGAGCTGGCA GAGGCCCCTC GGTTTGAGTC CATCATGGAG 
GACGTGGAGG TGGGGGCTGG GGAAACTGCT CGCTTTGCGG TGGTGGTCGA GGGAAAACCA 
CTGCCGGACA TCATGTGGTA CAAGGACGAG GTGCTGCTGA CCGAGAGCAG CCATGTGAGC 
TTCGTGTACG AGGAGAATGA GTGCTCCCTG GTGGTGCTCA GCACGGGGGC CCAGGATGGA 
GGCGTCTACA CCTGCACCGC CCAGAACCTG GCGGGTGAGG TCTCCTGCAA AGCAGAGTTG 
GCTGTGCATT CAGCTCAGAC AGCTATGGAG GTCGAGGGGG TCGGGGAGGA TGAGGACCAT 
CGAGGAAGGA GACTCAGCGA CTTTTATGAC ATCCACCAGG AGATCGGCAG GGGTGCTTTC 
TCCTACTTGC GGCGCATAGT GGAGCGTAGC TCCGGCCTGG AGTTTGCGGC CAAGTTCATC 
CCCAGCCAGG CCAAGCCAAA GGCATCAGCG CGTCGGGAGG CCCGGCTGCT GGCCAGGCTC 
CAGCACGACT GTGTCCTCTA CTTCCATGAG GCCTTCGAGA GGCGCCGGGG ACTGGTCATT 
GTCACCGAGC TCTGCACAGA GGAGCTGCTG GAGCGAATCG CCAGGAAACC CACCGTGTGT 
GAGTCTGAGA TCCGGGCCTA TATGCGGCAG GTGCTAGAGG GAATACACTA CCTGCACCAG 
AGCCACGTGC TGCACCTCGA TGTCAAGCCT GAGAACCTGC TGGTGTGGGA TGGTGCTGCG 
GGCGAGCAGC AGGTGCGGAT CTGTGACTTT GGGAATGCCC AGGAGCTGAC TCCAGGAGAG 
CCCCAGTACT GCCAGTATGG CACACCTGAG TTTGTAGCAC CCGAGATTGT CAATCAGAGC 
CCCGTGTCTG GAGTCACTGA CATCTGGCCT GTGGGTGTTG TTGCCTTCCT CTGTCTGACA 
GGAATCTCCC CGTTTGTTGG GGAAAATGAC CGGACAACAT TGATGAACAT CCGAAACTAC 
AACGTGGCCT TCGAGGAGAC CACATTCCTG AGCCTGAGCA GGGAGGCCCG GGGCTTCCTC 
ATCAAAGTGT TGGTGCAGGA CCGGCTGAGA CCTACCGCAG AAGAGACCCT AGAACATCCT 
TGGTTCAAAA CTCAGGCAAA GGGCGCAGAG GTGAGCACGG ATCACCTGAA GCTATTCCTC 
TCCCGGCGGA GGTGGCAGCG CTCCCAGATC AGCTACAAAT GCCACCTGGT GCTGCGCCCC 
ATCCCCGAGC TGCTGCGGGC CCCCCCAGAG CGGGTGTGGG TGACCATGCC CAGAAGGCCA 
CCCCCCAGTG GGGGGCTCTC ATCCTCCTCG GATTCTGAAG AGGAAGAGCT GGAAGAGCTG 
CCCTCAGTGC CCCGCCCACT GCAGCCCGAG TTCTCTGGCT CCCGGGTGTC CCTCACAGAC 
ATTCCCACTG AGGATGAGGC CCTGGGGACC CCAGAGACTG GGGCTGCCAC CCCCATGGAC 
TGGCAGGAGC AGGGAAGGGC TCCCTCTCAG GACCAGGAGG CTCCCAGCCC AGAGGCCCTC 
CCCTCCCCAG GCCAGGAGCC CGCAGCTGGG GCTAGCCCCA GGCGGGGAGA GCTCCGCAGG 
GGCAGCTCGG CTGAGAGCGC CCTGCCCCGG GCCGGGCCGC GGGAGCTGGG CCGGGGCCTG 
CACAAGGCGG CGTCTGTGGA GCTGCCGCAG CGCCGGAGCC CCAGCCCGGG AGCCACCCGC 
CTGGCCCGGG GAGGCCTGGG TGAGGGCGAG TATGCCCAGA GGCTGCAGGC CCTGCGCCAG 
CGGCTGCTGC GGGGAGGCCC CGAGGATGGC AAGGTCAGCG GCCTCAGGGG TCCCCTGCTG 
GAGAGCCTGG GGGGCCGTGC TCGGGACCCC CGGATGGCAC GAGCTGCCTC CAGCGAGGCA 
GCGCCCCACC ACCAGCCCCC ACTCGAGAAC CGGGGCCTGC AAAAGAGCAG CAGCTTCTCC 
CAGGGTGAGG CGGAGCCCCG GGGCCGGCAC CGCCGAGCGG GGGCGCCCCT CGAGATCCCC 
GTGGCCAGGC TTGGGGCCCG TAGGCTACAG GAGTCTCCTT CCCTGTCTGC CCTCAGCGAG 
GCCCAGCCAT CCAGCCCTGC ACGGCCCAGC GCCCCCAAAC CCAGTACCCC TAAGTCTGCA 
GAACCTTCTG CCACCACACC TAGTGATGCT CCGCAGCCCC CCGCACCCCA GCCTGCCCAA 
GACAAGGCTC CAGAGCCCAG GCCAGAACCA GTCCGAGCCT CCAAGCCTGC ACCACCCCCC 
CAGGCCCTGC AAACCCTAGC GCTGCCCCTC ACACCCTATG CTCAGATCAT TCAGTCCCTC 
CAGCTGTCAG GCCACGCCCA GGGCCCCTCG CAGGGCCCTG CCGCGCCGCC TTCAGAGCCC 
AAGCCCCACG CTGCTGTCTT TGCCAGGGTG GCCTCCCCAC CTCCGGGAGC CCCCGAGAAG 
CGCGTGCCCT CAGCCGGGGG TCCCCCGGTG CTAGCCGAGA AAGCCCGAGT TCCCACGGTG 
CCCCCCAGGC CAGGCAGCAG TCTCAGTAGC AGCATCGAAA ACTTGGAGTC GGAGGCCGTG 
TTCGAGGCCA AGTTCAAGCG CAGCCGCGAG TCGCCCCTGT CGCTGGGGCT GCGGCTGCTG 
AGCCGTTCGC GCTCGGAGGA GCGCGGCCCC TTCCGTGGGG CCGAGGAGGA GGATGGCATA 
TACCGGCCCA GCCCGGCGGG GACCCCGCTG GAGCTGGTGC GACGGCCTGA GCGCTCACGC 
TCGGTGCAGG ACCTCAGGGC TGTCGGAGAG CCTGGCCTCG TCCGCCGCCT CTCGCTGTCA 
CTGTCCCAGC GGCTGCGGCG GACCCCTCCC GCGCAGCGCC ACCCGGCCTG GGAGGCCCGC 
GGCGGGGACG GAGAGAGCTC GGAGGGCGGG AGCTCGGCGC GGGGCTCCCC GGTGCTGGCG 
ATGCGCAGGC GGCTGAGCTT CACCCTGGAG CGGCTGTCCA GCCGATTGCA GCGCAGTGGC 
AGCAGCGAGG ACTCGGGGGG CGCGTCGGGC CGCAGCACGC CGCTGTTCGG ACGGCTTCGC 
AGGGCCACGT CCGAGGGCGA GAGTCTGCGG CGCCTTGGCC TTCCGCACAA CCAGTTGGCC 
GCCCAGGCCG GCGCCACCAC GCCTTCCGCC GAGTCCCTGG GCTCCGAGGC CAGCGCCACG 
TCGGGCTCCT CAGCCCCAGG GGAAAGCCGA AGCCGGCTCC GCTGGGGCTT CTCTCGGCCG 
CGGAAGGACA AGGGGTTATC GCCACCAAAC CTCTCTGCCA GCGTCCAGGA GGAGTTGGGT 
CACCAGTACG TGCGCAGTGA GTCAGACTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGC TGGAGGGGGA GGCAGCCACC CTGCTCTGCC TGCCAGCGGC CTGCCCTGCA 
CCGCACATCT CCTGGATGAA AGACAAGAAG TCCTTGAGGT CAGAGCCCTC AGTGATCATC 
GTGTCCTGCA AAGATGGGCG GCAGCTGCTC AGCATCCCCC GGGCGGGCAA GCGGCACGCC 
GGTCTCTATG AGTGCTCGGC CACCAACGTA CTGGGCAGCA TCACCAGCTC CTGTACCGTG 
GCTGTGGCCC GAGTCCCAGG AAAGCTAGCT CCTCCAGAGG TACCCCAGAC CTACCAGGAC 
ACGGCGCTGG TGCTGTGGAA GCCGGGAGAC AGCCGGGCAC CTTGCACGTA TACGCTGGAG 
CGGCGAGTGG ATGGGGAGTC TGTGTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAC 
TACAACGTGA CCCACCTGCC AGTTGGCGTG ACTGTGAGGT TCCGTGTGGC CTGTGCCAAC 
CGTGCTGGGC AGGGGCCCTT CAGCAACTCT TCTGAGAAGG TCTTTGTCAG GGGTACTCAA 
GATTCTTCAG CTGTGCCATC TGCTGCCCAC CAAGAGGCCC CTGTCACCTC AAGGCCAGCC 
AGGGCCCGGC CTCCTGACTC TCCTACCTCA CTGGCCCCAC CCCTAGCTCC TGCTGCCCCC 
ACACCCCCGT CAGTCACTGT CAGCCCCTCA TCTCCCCCCA CACCTCCTAG CCAGGCCTTG 
TCCTCGCTCA AGGCTGTGGG TCCACCACCC CAAACCCCTC CACGAAGACA CAGGGGCCTG 
CAGGCTGCCC GGCCAGCGGA GCCCACCCTA CCCAGTACCC ACGTCACCCC AAGTGAGCCC 
AAGCCTTTCG TCCTTGACAC TGGGACCCCG ATCCCAGCCT CCACTCCTCA AGGGGTTAAA 
CCAGTGTCTT CCTCTACTCC TGTGTATGTG GTGACTTCCT TTGTGTCTGC ACCACCAGCC 
CCTGAGCCCC CAGCCCCTGA GCCCCCTCCT GAGCCTACCA AGGTGACTGT GCAGAGCCTC 
AGCCCGGCCA AGGAGGTGGT CAGCTCCCCT GGGAGCAGTC CCCGAAGCTC TCCCAGGCCT 
GAGGGTACCA CTCTTCGACA GGGTCCCCCT CAGAAACCCT ACACCTTCCT GGAGGAGAAA 
GCCAGGGGCC GCTTTGGTGT TGTGCGAGCG TGCCGGGAGA ATGCCACGGG GCGAACGTTC 
GTGGCCAAGA TCGTGCCCTA TGCTGCCGAG GGCAAGCGGC GGGTCCTGCA GGAGTACGAG 
GTGCTGCGGA CCCTGCACCA CGAGCGGATC ATGTCCCTGC ACGAGGCCTA CATCACCCCT 
CGGTACCTCG TGCTCATTGC TGAGAGCTGT GGCAACCGGG AACTCCTCTG TGGGCTCAGT 
GACAGGTTCC GGTATTCTGA GGATGACGTG GCCACTTACA TGGTGCAGCT GCTACAAGGC 
CTGGACTACC TCCACGGCCA CCACGTGCTC CACCTAGACA TCAAGCCAGA CAACCTGCTG 
CTGGCCCCTG ACAATGCCCT CAAGATTGTG GACTTTGGCA GTGCCCAGCC CTACAACCCC 
CAGGCCCTTA GGCCCCTTGG CCACCGCACG GGCACGCTGG AGTTCATGGC TCCGGAGATG 
GTGAAGGGAG AACCCATCGG CTCTGCCACG GACATCTGGG GAGCGGGTGT GCTCACTTAC 
ATTATGCTCA GTGGACGCTC CCCGTTCTAT GAGCCAGACC CCCAGGAAAC GGAGGCTCGG 
ATTGTGGGGG GCCGCTTTGA TGCCTTCCAG CTGTACCCCA ATACATCCCA GAGCGCCACC 
CTCTTCTTGC GAAAGGTTCT CTCTGTACAT CCCTGGAGCC GGCCCTCCCT GCAGGACTGC 
CTGGCCCACC CATGGTTGCA GGACGCCTAC CTGATGAAGC TGCGCCGCCA GACGCTCACC 
TTCACCACCA ACCGGCTCAA GGAGTTCCTG GGCGAGCAGC GGCGGCGCCG GGCTGAGGCT 
GCCACCCGCC ACAAGGTGCT GCTGCGCTCC TACCCTGGCG GCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005246296.1
CDS	1920..9347
Translation	MATATNELGQATCAASLTVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPTDVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARFDCKISGTPPPVVTWTHFGCPMEESENLRLRQDGGLHSLHIAHVGSEDEGLYAVSAVNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEQGELERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDVHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPQVVAVTGRMVTLTWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPGWAATGLRKGVQHIFRVLSTTVKSSSKPSPPSEPVQLLEHGPTLEEAPAMLDKPDIVYVVEGQPASVTVTFNHVEAQVVWRSCRGALLEARAGVYELSQPDDDQYCLRICRVSRRDMGALTCTARNRHGTQTCSVTLELAEAPRFESIMEDVEVGAGETARFAVVVEGKPLPDIMWYKDEVLLTESSHVSFVYEENECSLVVLSTGAQDGGVYTCTAQNLAGEVSCKAELAVHSAQTAMEVEGVGEDEDHRGRRLSDFYDIHQEIGRGAFSYLRRIVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHDCVLYFHEAFERRRGLVIVTELCTEELLERIARKPTVCESEIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTQAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGELRRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQPPAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPSEPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEAKFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAVGEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAESLGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPHISWMKDKKSLRSEPSVIIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARVPGKLAPPEVPQTYQDTALVLWKPGDSRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTHLPVGVTVRFRVACANRAGQGPFSNSSEKVFVRGTQDSSAVPSAAHQEAPVTSRPARARPPDSPTSLAPPLAPAAPTPPSVTVSPSSPPTPPSQALSSLKAVGPPPQTPPRRHRGLQAARPAEPTLPSTHVTPSEPKPFVLDTGTPIPASTPQGVKPVSSSTPVYVVTSFVSAPPAPEPPAPEPPPEPTKVTVQSLSPAKEVVSSPGSSPRSSPRPEGTTLRQGPPQKPYTFLEEKARGRFGVVRACRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERIMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYMVQLLQGLDYLHGHHVLHLDIKPDNLLLAPDNALKIVDFGSAQPYNPQALRPLGHRTGTLEFMAPEMVKGEPIGSATDIWGAGVLTYIMLSGRSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGGP

Target ORF information:

RefSeq Version	XM_005246239.2
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246239.2

ORF Insert Sequence:

ATGGCCACCG CCACCAACGA GCTGGGCCAG GCCACCTGTG CCGCCTCACT GACCGTGAGA 
CCCGGTGGGT CTACATCCCC TTTCAGCAGC CCCATCACCT CCGACGAGGA ATACCTGAGC 
CCCCCAGAGG AGTTCCCAGA GCCTGGGGAG ACCTGGCCGC GAACCCCCAC CATGAAGCCC 
AGTCCCAGCC AGAACCGCCG TTCTTCTGAC ACTGGCTCCA AGGCACCCCC CACCTTCAAG 
GTCTCACTTA TGGACCAGTC AGTAAGAGAA GGCCAAGATG TCATCATGAG CATCCGCGTG 
CAGGGGGAGC CCAAGCCTGT GGTCTCCTGG CTGAGAAACC GCCAGCCCGT GCGCCCAGAC 
CAGCGGCGCT TTGCGGAGGA GGCTGAGGGT GGGCTGTGCC GGCTGCGGAT CCTGGCTGCA 
GAGCGTGGCG ATGCTGGTTT CTACACTTGC AAAGCGGTCA ATGAGTATGG TGCTCGGCAG 
TGCGAGGCCC GCTTGGAGGT CCGAGCACAC CCTGAAAGCC GGTCCCTGGC CGTGCTGGCC 
CCCCTGCAGG ACGTGGACGT GGGGGCCGGG GAGATGGCGC TGTTTGAGTG CCTGGTGGCG 
GGGCCCACTG ACGTGGAGGT GGATTGGCTG TGCCGTGGCC GCCTGCTGCA GCCTGCACTG 
CTCAAATGCA AGATGCATTT CGATGGCCGC AAATGCAAGC TGCTACTTAC ATCTGTACAT 
GAGGACGACA GTGGCGTCTA CACCTGCAAG CTCAGCACGG CCAAAGATGA GCTGACCTGC 
AGTGCCCGGC TGACCGTGCG GCCCTCGTTG GCACCCCTGT TCACACGGCT GCTGGAAGAT 
GTGGAGGTGT TGGAGGGCCG AGCTGCCCGT TTCGACTGCA AGATCAGTGG CACCCCGCCC 
CCTGTTGTTA CCTGGACTCA TTTTGGCTGC CCCATGGAGG AGAGTGAGAA CTTGCGGCTG 
CGGCAGGACG GGGGTCTGCA CTCACTGCAC ATTGCCCATG TGGGCAGCGA GGACGAGGGG 
CTCTATGCGG TCAGTGCTGT TAACACCCAT GGCCAGGCCC ACTGCTCAGC CCAGCTGTAT 
GTAGAAGAGC CCCGGACAGC CGCCTCAGGC CCCAGCTCGA AGCTGGAGAA GATGCCATCC 
ATTCCCGAGG AGCCAGAGCA GGGTGAGCTG GAGCGGCTGT CCATTCCTGA CTTCCTGCGG 
CCACTGCAGG ACCTGGAGGT GGGACTGGCC AAGGAGGCCA TGCTAGAGTG CCAGGTGACC 
GGCCTGCCCT ACCCCACCAT CAGCTGGTTC CACAATGGCC ACCGCATCCA GAGCAGCGAC 
GACCGGCGCA TGACACAGTA CAGGGATGTC CATCGCTTGG TGTTCCCTGC CGTGGGGCCT 
CAGCACGCCG GTGTCTACAA GAGCGTCATT GCCAACAAGC TGGGCAAAGC TGCCTGCTAT 
GCCCACCTGT ATGTCACAGA TGTGGTCCCA GGCCCTCCAG ATGGCGCCCC GCAGGTGGTG 
GCTGTGACGG GGAGGATGGT CACACTCACA TGGAACCCCC CCAGGAGTCT GGACATGGCC 
ATCGACCCGG ACTCCCTGAC GTACACAGTG CAGCACCAGG TGCTGGGCTC GGACCAGTGG 
ACGGCACTGG TCACAGGCCT GCGGGAGCCA GGGTGGGCAG CCACAGGGCT GCGTAAGGGG 
GTCCAGCACA TCTTCCGGGT CCTCAGCACC ACTGTCAAGA GCAGCAGCAA GCCCTCACCC 
CCTTCTGAGC CTGTGCAGCT GCTGGAGCAC GGCCCAACCC TGGAGGAGGC CCCTGCCATG 
CTGGACAAAC CAGACATCGT GTATGTGGTG GAGGGACAGC CTGCCAGCGT CACCGTCACA 
TTCAACCATG TGGAGGCCCA GGTCGTCTGG AGGAGCTGCC GAGGGGCCCT CCTAGAGGCA 
CGGGCCGGTG TGTACGAGCT GAGCCAGCCA GATGATGACC AGTACTGTCT TCGGATCTGC 
CGGGTGAGCC GCCGGGACAT GGGGGCCCTC ACCTGCACCG CCCGAAACCG TCACGGCACA 
CAGACCTGCT CGGTCACATT GGAGCTGGCA GAGGCCCCTC GGTTTGAGTC CATCATGGAG 
GACGTGGAGG TGGGGGCTGG GGAAACTGCT CGCTTTGCGG TGGTGGTCGA GGGAAAACCA 
CTGCCGGACA TCATGTGGTA CAAGGACGAG GTGCTGCTGA CCGAGAGCAG CCATGTGAGC 
TTCGTGTACG AGGAGAATGA GTGCTCCCTG GTGGTGCTCA GCACGGGGGC CCAGGATGGA 
GGCGTCTACA CCTGCACCGC CCAGAACCTG GCGGGTGAGG TCTCCTGCAA AGCAGAGTTG 
GCTGTGCATT CAGCTCAGAC AGCTATGGAG GTCGAGGGGG TCGGGGAGGA TGAGGACCAT 
CGAGGAAGGA GACTCAGCGA CTTTTATGAC ATCCACCAGG AGATCGGCAG GGGTGCTTTC 
TCCTACTTGC GGCGCATAGT GGAGCGTAGC TCCGGCCTGG AGTTTGCGGC CAAGTTCATC 
CCCAGCCAGG CCAAGCCAAA GGCATCAGCG CGTCGGGAGG CCCGGCTGCT GGCCAGGCTC 
CAGCACGACT GTGTCCTCTA CTTCCATGAG GCCTTCGAGA GGCGCCGGGG ACTGGTCATT 
GTCACCGAGC TCTGCACAGA GGAGCTGCTG GAGCGAATCG CCAGGAAACC CACCGTGTGT 
GAGTCTGAGA TCCGGGCCTA TATGCGGCAG GTGCTAGAGG GAATACACTA CCTGCACCAG 
AGCCACGTGC TGCACCTCGA TGTCAAGCCT GAGAACCTGC TGGTGTGGGA TGGTGCTGCG 
GGCGAGCAGC AGGTGCGGAT CTGTGACTTT GGGAATGCCC AGGAGCTGAC TCCAGGAGAG 
CCCCAGTACT GCCAGTATGG CACACCTGAG TTTGTAGCAC CCGAGATTGT CAATCAGAGC 
CCCGTGTCTG GAGTCACTGA CATCTGGCCT GTGGGTGTTG TTGCCTTCCT CTGTCTGACA 
GGAATCTCCC CGTTTGTTGG GGAAAATGAC CGGACAACAT TGATGAACAT CCGAAACTAC 
AACGTGGCCT TCGAGGAGAC CACATTCCTG AGCCTGAGCA GGGAGGCCCG GGGCTTCCTC 
ATCAAAGTGT TGGTGCAGGA CCGGCTGAGA CCTACCGCAG AAGAGACCCT AGAACATCCT 
TGGTTCAAAA CTCAGGCAAA GGGCGCAGAG GTGAGCACGG ATCACCTGAA GCTATTCCTC 
TCCCGGCGGA GGTGGCAGCG CTCCCAGATC AGCTACAAAT GCCACCTGGT GCTGCGCCCC 
ATCCCCGAGC TGCTGCGGGC CCCCCCAGAG CGGGTGTGGG TGACCATGCC CAGAAGGCCA 
CCCCCCAGTG GGGGGCTCTC ATCCTCCTCG GATTCTGAAG AGGAAGAGCT GGAAGAGCTG 
CCCTCAGTGC CCCGCCCACT GCAGCCCGAG TTCTCTGGCT CCCGGGTGTC CCTCACAGAC 
ATTCCCACTG AGGATGAGGC CCTGGGGACC CCAGAGACTG GGGCTGCCAC CCCCATGGAC 
TGGCAGGAGC AGGGAAGGGC TCCCTCTCAG GACCAGGAGG CTCCCAGCCC AGAGGCCCTC 
CCCTCCCCAG GCCAGGAGCC CGCAGCTGGG GCTAGCCCCA GGCGGGGAGA GCTCCGCAGG 
GGCAGCTCGG CTGAGAGCGC CCTGCCCCGG GCCGGGCCGC GGGAGCTGGG CCGGGGCCTG 
CACAAGGCGG CGTCTGTGGA GCTGCCGCAG CGCCGGAGCC CCAGCCCGGG AGCCACCCGC 
CTGGCCCGGG GAGGCCTGGG TGAGGGCGAG TATGCCCAGA GGCTGCAGGC CCTGCGCCAG 
CGGCTGCTGC GGGGAGGCCC CGAGGATGGC AAGGTCAGCG GCCTCAGGGG TCCCCTGCTG 
GAGAGCCTGG GGGGCCGTGC TCGGGACCCC CGGATGGCAC GAGCTGCCTC CAGCGAGGCA 
GCGCCCCACC ACCAGCCCCC ACTCGAGAAC CGGGGCCTGC AAAAGAGCAG CAGCTTCTCC 
CAGGGTGAGG CGGAGCCCCG GGGCCGGCAC CGCCGAGCGG GGGCGCCCCT CGAGATCCCC 
GTGGCCAGGC TTGGGGCCCG TAGGCTACAG GAGTCTCCTT CCCTGTCTGC CCTCAGCGAG 
GCCCAGCCAT CCAGCCCTGC ACGGCCCAGC GCCCCCAAAC CCAGTACCCC TAAGTCTGCA 
GAACCTTCTG CCACCACACC TAGTGATGCT CCGCAGCCCC CCGCACCCCA GCCTGCCCAA 
GACAAGGCTC CAGAGCCCAG GCCAGAACCA GTCCGAGCCT CCAAGCCTGC ACCACCCCCC 
CAGGCCCTGC AAACCCTAGC GCTGCCCCTC ACACCCTATG CTCAGATCAT TCAGTCCCTC 
CAGCTGTCAG GCCACGCCCA GGGCCCCTCG CAGGGCCCTG CCGCGCCGCC TTCAGAGCCC 
AAGCCCCACG CTGCTGTCTT TGCCAGGGTG GCCTCCCCAC CTCCGGGAGC CCCCGAGAAG 
CGCGTGCCCT CAGCCGGGGG TCCCCCGGTG CTAGCCGAGA AAGCCCGAGT TCCCACGGTG 
CCCCCCAGGC CAGGCAGCAG TCTCAGTAGC AGCATCGAAA ACTTGGAGTC GGAGGCCGTG 
TTCGAGGCCA AGTTCAAGCG CAGCCGCGAG TCGCCCCTGT CGCTGGGGCT GCGGCTGCTG 
AGCCGTTCGC GCTCGGAGGA GCGCGGCCCC TTCCGTGGGG CCGAGGAGGA GGATGGCATA 
TACCGGCCCA GCCCGGCGGG GACCCCGCTG GAGCTGGTGC GACGGCCTGA GCGCTCACGC 
TCGGTGCAGG ACCTCAGGGC TGTCGGAGAG CCTGGCCTCG TCCGCCGCCT CTCGCTGTCA 
CTGTCCCAGC GGCTGCGGCG GACCCCTCCC GCGCAGCGCC ACCCGGCCTG GGAGGCCCGC 
GGCGGGGACG GAGAGAGCTC GGAGGGCGGG AGCTCGGCGC GGGGCTCCCC GGTGCTGGCG 
ATGCGCAGGC GGCTGAGCTT CACCCTGGAG CGGCTGTCCA GCCGATTGCA GCGCAGTGGC 
AGCAGCGAGG ACTCGGGGGG CGCGTCGGGC CGCAGCACGC CGCTGTTCGG ACGGCTTCGC 
AGGGCCACGT CCGAGGGCGA GAGTCTGCGG CGCCTTGGCC TTCCGCACAA CCAGTTGGCC 
GCCCAGGCCG GCGCCACCAC GCCTTCCGCC GAGTCCCTGG GCTCCGAGGC CAGCGCCACG 
TCGGGCTCCT CAGCCCCAGG GGAAAGCCGA AGCCGGCTCC GCTGGGGCTT CTCTCGGCCG 
CGGAAGGACA AGGGGTTATC GCCACCAAAC CTCTCTGCCA GCGTCCAGGA GGAGTTGGGT 
CACCAGTACG TGCGCAGTGA GTCAGACTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGC TGGAGGGGGA GGCAGCCACC CTGCTCTGCC TGCCAGCGGC CTGCCCTGCA 
CCGCACATCT CCTGGATGAA AGACAAGAAG TCCTTGAGGT CAGAGCCCTC AGTGATCATC 
GTGTCCTGCA AAGATGGGCG GCAGCTGCTC AGCATCCCCC GGGCGGGCAA GCGGCACGCC 
GGTCTCTATG AGTGCTCGGC CACCAACGTA CTGGGCAGCA TCACCAGCTC CTGTACCGTG 
GCTGTGGCCC GAGTCCCAGG AAAGCTAGCT CCTCCAGAGG TACCCCAGAC CTACCAGGAC 
ACGGCGCTGG TGCTGTGGAA GCCGGGAGAC AGCCGGGCAC CTTGCACGTA TACGCTGGAG 
CGGCGAGTGG ATGGGGAGTC TGTGTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAC 
TACAACGTGA CCCACCTGCC AGTTGGCGTG ACTGTGAGGT TCCGTGTGGC CTGTGCCAAC 
CGTGCTGGGC AGGGGCCCTT CAGCAACTCT TCTGAGAAGG TCTTTGTCAG GGGTACTCAA 
GATTCTTCAG CTGTGCCATC TGCTGCCCAC CAAGAGGCCC CTGTCACCTC AAGGCCAGCC 
AGGGCCCGGC CTCCTGACTC TCCTACCTCA CTGGCCCCAC CCCTAGCTCC TGCTGCCCCC 
ACACCCCCGT CAGTCACTGT CAGCCCCTCA TCTCCCCCCA CACCTCCTAG CCAGGCCTTG 
TCCTCGCTCA AGGCTGTGGG TCCACCACCC CAAACCCCTC CACGAAGACA CAGGGGCCTG 
CAGGCTGCCC GGCCAGCGGA GCCCACCCTA CCCAGTACCC ACGTCACCCC AAGTGAGCCC 
AAGCCTTTCG TCCTTGACAC TGGGACCCCG ATCCCAGCCT CCACTCCTCA AGGGGTTAAA 
CCAGTGTCTT CCTCTACTCC TGTGTATGTG GTGACTTCCT TTGTGTCTGC ACCACCAGCC 
CCTGAGCCCC CAGCCCCTGA GCCCCCTCCT GAGCCTACCA AGGTGACTGT GCAGAGCCTC 
AGCCCGGCCA AGGAGGTGGT CAGCTCCCCT GGGAGCAGTC CCCGAAGCTC TCCCAGGCCT 
GAGGGTACCA CTCTTCGACA GGGTCCCCCT CAGAAACCCT ACACCTTCCT GGAGGAGAAA 
GCCAGGGGCC GCTTTGGTGT TGTGCGAGCG TGCCGGGAGA ATGCCACGGG GCGAACGTTC 
GTGGCCAAGA TCGTGCCCTA TGCTGCCGAG GGCAAGCGGC GGGTCCTGCA GGAGTACGAG 
GTGCTGCGGA CCCTGCACCA CGAGCGGATC ATGTCCCTGC ACGAGGCCTA CATCACCCCT 
CGGTACCTCG TGCTCATTGC TGAGAGCTGT GGCAACCGGG AACTCCTCTG TGGGCTCAGT 
GACAGGTTCC GGTATTCTGA GGATGACGTG GCCACTTACA TGGTGCAGCT GCTACAAGGC 
CTGGACTACC TCCACGGCCA CCACGTGCTC CACCTAGACA TCAAGCCAGA CAACCTGCTG 
CTGGCCCCTG ACAATGCCCT CAAGATTGTG GACTTTGGCA GTGCCCAGCC CTACAACCCC 
CAGGCCCTTA GGCCCCTTGG CCACCGCACG GGCACGCTGG AGTTCATGGC TCCGGAGATG 
GTGAAGGGAG AACCCATCGG CTCTGCCACG GACATCTGGG GAGCGGGTGT GCTCACTTAC 
ATTATGCTCA GTGGACGCTC CCCGTTCTAT GAGCCAGACC CCCAGGAAAC GGAGGCTCGG 
ATTGTGGGGG GCCGCTTTGA TGCCTTCCAG CTGTACCCCA ATACATCCCA GAGCGCCACC 
CTCTTCTTGC GAAAGGTTCT CTCTGTACAT CCCTGGAGCC GGCCCTCCCT GCAGGACTGC 
CTGGCCCACC CATGGTTGCA GGACGCCTAC CTGATGAAGC TGCGCCGCCA GACGCTCACC 
TTCACCACCA ACCGGCTCAA GGAGTTCCTG GGCGAGCAGC GGCGGCGCCG GGCTGAGGCT 
GCCACCCGCC ACAAGGTGCT GCTGCGCTCC TACCCTGGCG GCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_005246242.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005246299.1 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X9, mRNA.

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CloneID	OHu35757
Clone ID Related Accession (Same CDS sequence)	XM_005246242.4
Accession Version	XM_005246242.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005246242.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGTCC TAGCCATGCT GCTCCAGGGT TCCATAGACC TGCTTCCCCT GCTTTTGGAG 
CCTGCAGTTG GTAGTTTAGG TGGGTCTACA TCCCCTTTCA GCAGCCCCAT CACCTCCGAC 
GAGGAATACC TGAGCCCCCC AGAGGAGTTC CCAGAGCCTG GGGAGACCTG GCCGCGAACC 
CCCACCATGA AGCCCAGTCC CAGCCAGAAC CGCCGTTCTT CTGACACTGG CTCCAAGGCA 
CCCCCCACCT TCAAGGTCTC ACTTATGGAC CAGTCAGTAA GAGAAGGCCA AGATGTCATC 
ATGAGCATCC GCGTGCAGGG GGAGCCCAAG CCTGTGGTCT CCTGGCTGAG AAACCGCCAG 
CCCGTGCGCC CAGACCAGCG GCGCTTTGCG GAGGAGGCTG AGGGTGGGCT GTGCCGGCTG 
CGGATCCTGG CTGCAGAGCG TGGCGATGCT GGTTTCTACA CTTGCAAAGC GGTCAATGAG 
TATGGTGCTC GGCAGTGCGA GGCCCGCTTG GAGGTCCGAG CACACCCTGA AAGCCGGTCC 
CTGGCCGTGC TGGCCCCCCT GCAGGACGTG GACGTGGGGG CCGGGGAGAT GGCGCTGTTT 
GAGTGCCTGG TGGCGGGGCC CACTGACGTG GAGGTGGATT GGCTGTGCCG TGGCCGCCTG 
CTGCAGCCTG CACTGCTCAA ATGCAAGATG CATTTCGATG GCCGCAAATG CAAGCTGCTA 
CTTACATCTG TACATGAGGA CGACAGTGGC GTCTACACCT GCAAGCTCAG CACGGCCAAA 
GATGAGCTGA CCTGCAGTGC CCGGCTGACC GTGCGGCCCT CGTTGGCACC CCTGTTCACA 
CGGCTGCTGG AAGATGTGGA GGTGTTGGAG GGCCGAGCTG CCCGTTTCGA CTGCAAGATC 
AGTGGCACCC CGCCCCCTGT TGTTACCTGG ACTCATTTTG GCTGCCCCAT GGAGGAGAGT 
GAGAACTTGC GGCTGCGGCA GGACGGGGGT CTGCACTCAC TGCACATTGC CCATGTGGGC 
AGCGAGGACG AGGGGCTCTA TGCGGTCAGT GCTGTTAACA CCCATGGCCA GGCCCACTGC 
TCAGCCCAGC TGTATGTAGA AGAGCCCCGG ACAGCCGCCT CAGGCCCCAG CTCGAAGCTG 
GAGAAGATGC CATCCATTCC CGAGGAGCCA GAGCAGGGTG AGCTGGAGCG GCTGTCCATT 
CCTGACTTCC TGCGGCCACT GCAGGACCTG GAGGTGGGAC TGGCCAAGGA GGCCATGCTA 
GAGTGCCAGG TGACCGGCCT GCCCTACCCC ACCATCAGCT GGTTCCACAA TGGCCACCGC 
ATCCAGAGCA GCGACGACCG GCGCATGACA CAGTACAGGG ATGTCCATCG CTTGGTGTTC 
CCTGCCGTGG GGCCTCAGCA CGCCGGTGTC TACAAGAGCG TCATTGCCAA CAAGCTGGGC 
AAAGCTGCCT GCTATGCCCA CCTGTATGTC ACAGATGTGG TCCCAGGCCC TCCAGATGGC 
GCCCCGCAGG TGGTGGCTGT GACGGGGAGG ATGGTCACAC TCACATGGAA CCCCCCCAGG 
AGTCTGGACA TGGCCATCGA CCCGGACTCC CTGACGTACA CAGTGCAGCA CCAGGTGCTG 
GGCTCGGACC AGTGGACGGC ACTGGTCACA GGCCTGCGGG AGCCAGGGTG GGCAGCCACA 
GGGCTGCGTA AGGGGGTCCA GCACATCTTC CGGGTCCTCA GCACCACTGT CAAGAGCAGC 
AGCAAGCCCT CACCCCCTTC TGAGCCTGTG CAGCTGCTGG AGCACGGCCC AACCCTGGAG 
GAGGCCCCTG CCATGCTGGA CAAACCAGAC ATCGTGTATG TGGTGGAGGG ACAGCCTGCC 
AGCGTCACCG TCACATTCAA CCATGTGGAG GCCCAGGTCG TCTGGAGGAG CTGCCGAGGG 
GCCCTCCTAG AGGCACGGGC CGGTGTGTAC GAGCTGAGCC AGCCAGATGA TGACCAGTAC 
TGTCTTCGGA TCTGCCGGGT GAGCCGCCGG GACATGGGGG CCCTCACCTG CACCGCCCGA 
AACCGTCACG GCACACAGAC CTGCTCGGTC ACATTGGAGC TGGCAGAGGC CCCTCGGTTT 
GAGTCCATCA TGGAGGACGT GGAGGTGGGG GCTGGGGAAA CTGCTCGCTT TGCGGTGGTG 
GTCGAGGGAA AACCACTGCC GGACATCATG TGGTACAAGG ACGAGGTGCT GCTGACCGAG 
AGCAGCCATG TGAGCTTCGT GTACGAGGAG AATGAGTGCT CCCTGGTGGT GCTCAGCACG 
GGGGCCCAGG ATGGAGGCGT CTACACCTGC ACCGCCCAGA ACCTGGCGGG TGAGGTCTCC 
TGCAAAGCAG AGTTGGCTGT GCATTCAGCT CAGACAGCTA TGGAGGTCGA GGGGGTCGGG 
GAGGATGAGG ACCATCGAGG AAGGAGACTC AGCGACTTTT ATGACATCCA CCAGGAGATC 
GGCAGGGGTG CTTTCTCCTA CTTGCGGCGC ATAGTGGAGC GTAGCTCCGG CCTGGAGTTT 
GCGGCCAAGT TCATCCCCAG CCAGGCCAAG CCAAAGGCAT CAGCGCGTCG GGAGGCCCGG 
CTGCTGGCCA GGCTCCAGCA CGACTGTGTC CTCTACTTCC ATGAGGCCTT CGAGAGGCGC 
CGGGGACTGG TCATTGTCAC CGAGCTCTGC ACAGAGGAGC TGCTGGAGCG AATCGCCAGG 
AAACCCACCG TGTGTGAGTC TGAGATCCGG GCCTATATGC GGCAGGTGCT AGAGGGAATA 
CACTACCTGC ACCAGAGCCA CGTGCTGCAC CTCGATGTCA AGCCTGAGAA CCTGCTGGTG 
TGGGATGGTG CTGCGGGCGA GCAGCAGGTG CGGATCTGTG ACTTTGGGAA TGCCCAGGAG 
CTGACTCCAG GAGAGCCCCA GTACTGCCAG TATGGCACAC CTGAGTTTGT AGCACCCGAG 
ATTGTCAATC AGAGCCCCGT GTCTGGAGTC ACTGACATCT GGCCTGTGGG TGTTGTTGCC 
TTCCTCTGTC TGACAGGAAT CTCCCCGTTT GTTGGGGAAA ATGACCGGAC AACATTGATG 
AACATCCGAA ACTACAACGT GGCCTTCGAG GAGACCACAT TCCTGAGCCT GAGCAGGGAG 
GCCCGGGGCT TCCTCATCAA AGTGTTGGTG CAGGACCGGC TGAGACCTAC CGCAGAAGAG 
ACCCTAGAAC ATCCTTGGTT CAAAACTCAG GCAAAGGGCG CAGAGGTGAG CACGGATCAC 
CTGAAGCTAT TCCTCTCCCG GCGGAGGTGG CAGCGCTCCC AGATCAGCTA CAAATGCCAC 
CTGGTGCTGC GCCCCATCCC CGAGCTGCTG CGGGCCCCCC CAGAGCGGGT GTGGGTGACC 
ATGCCCAGAA GGCCACCCCC CAGTGGGGGG CTCTCATCCT CCTCGGATTC TGAAGAGGAA 
GAGCTGGAAG AGCTGCCCTC AGTGCCCCGC CCACTGCAGC CCGAGTTCTC TGGCTCCCGG 
GTGTCCCTCA CAGACATTCC CACTGAGGAT GAGGCCCTGG GGACCCCAGA GACTGGGGCT 
GCCACCCCCA TGGACTGGCA GGAGCAGGGA AGGGCTCCCT CTCAGGACCA GGAGGCTCCC 
AGCCCAGAGG CCCTCCCCTC CCCAGGCCAG GAGCCCGCAG CTGGGGCTAG CCCCAGGCGG 
GGAGAGCTCC GCAGGGGCAG CTCGGCTGAG AGCGCCCTGC CCCGGGCCGG GCCGCGGGAG 
CTGGGCCGGG GCCTGCACAA GGCGGCGTCT GTGGAGCTGC CGCAGCGCCG GAGCCCCAGC 
CCGGGAGCCA CCCGCCTGGC CCGGGGAGGC CTGGGTGAGG GCGAGTATGC CCAGAGGCTG 
CAGGCCCTGC GCCAGCGGCT GCTGCGGGGA GGCCCCGAGG ATGGCAAGGT CAGCGGCCTC 
AGGGGTCCCC TGCTGGAGAG CCTGGGGGGC CGTGCTCGGG ACCCCCGGAT GGCACGAGCT 
GCCTCCAGCG AGGCAGCGCC CCACCACCAG CCCCCACTCG AGAACCGGGG CCTGCAAAAG 
AGCAGCAGCT TCTCCCAGGG TGAGGCGGAG CCCCGGGGCC GGCACCGCCG AGCGGGGGCG 
CCCCTCGAGA TCCCCGTGGC CAGGCTTGGG GCCCGTAGGC TACAGGAGTC TCCTTCCCTG 
TCTGCCCTCA GCGAGGCCCA GCCATCCAGC CCTGCACGGC CCAGCGCCCC CAAACCCAGT 
ACCCCTAAGT CTGCAGAACC TTCTGCCACC ACACCTAGTG ATGCTCCGCA GCCCCCCGCA 
CCCCAGCCTG CCCAAGACAA GGCTCCAGAG CCCAGGCCAG AACCAGTCCG AGCCTCCAAG 
CCTGCACCAC CCCCCCAGGC CCTGCAAACC CTAGCGCTGC CCCTCACACC CTATGCTCAG 
ATCATTCAGT CCCTCCAGCT GTCAGGCCAC GCCCAGGGCC CCTCGCAGGG CCCTGCCGCG 
CCGCCTTCAG AGCCCAAGCC CCACGCTGCT GTCTTTGCCA GGGTGGCCTC CCCACCTCCG 
GGAGCCCCCG AGAAGCGCGT GCCCTCAGCC GGGGGTCCCC CGGTGCTAGC CGAGAAAGCC 
CGAGTTCCCA CGGTGCCCCC CAGGCCAGGC AGCAGTCTCA GTAGCAGCAT CGAAAACTTG 
GAGTCGGAGG CCGTGTTCGA GGCCAAGTTC AAGCGCAGCC GCGAGTCGCC CCTGTCGCTG 
GGGCTGCGGC TGCTGAGCCG TTCGCGCTCG GAGGAGCGCG GCCCCTTCCG TGGGGCCGAG 
GAGGAGGATG GCATATACCG GCCCAGCCCG GCGGGGACCC CGCTGGAGCT GGTGCGACGG 
CCTGAGCGCT CACGCTCGGT GCAGGACCTC AGGGCTGTCG GAGAGCCTGG CCTCGTCCGC 
CGCCTCTCGC TGTCACTGTC CCAGCGGCTG CGGCGGACCC CTCCCGCGCA GCGCCACCCG 
GCCTGGGAGG CCCGCGGCGG GGACGGAGAG AGCTCGGAGG GCGGGAGCTC GGCGCGGGGC 
TCCCCGGTGC TGGCGATGCG CAGGCGGCTG AGCTTCACCC TGGAGCGGCT GTCCAGCCGA 
TTGCAGCGCA GTGGCAGCAG CGAGGACTCG GGGGGCGCGT CGGGCCGCAG CACGCCGCTG 
TTCGGACGGC TTCGCAGGGC CACGTCCGAG GGCGAGAGTC TGCGGCGCCT TGGCCTTCCG 
CACAACCAGT TGGCCGCCCA GGCCGGCGCC ACCACGCCTT CCGCCGAGTC CCTGGGCTCC 
GAGGCCAGCG CCACGTCGGG CTCCTCAGCC CCAGGGGAAA GCCGAAGCCG GCTCCGCTGG 
GGCTTCTCTC GGCCGCGGAA GGACAAGGGG TTATCGCCAC CAAACCTCTC TGCCAGCGTC 
CAGGAGGAGT TGGGTCACCA GTACGTGCGC AGTGAGTCAG ACTTCCCCCC AGTCTTCCAC 
ATCAAACTCA AGGACCAGGT GCTGCTGGAG GGGGAGGCAG CCACCCTGCT CTGCCTGCCA 
GCGGCCTGCC CTGCACCGCA CATCTCCTGG ATGAAAGACA AGAAGTCCTT GAGGTCAGAG 
CCCTCAGTGA TCATCGTGTC CTGCAAAGAT GGGCGGCAGC TGCTCAGCAT CCCCCGGGCG 
GGCAAGCGGC ACGCCGGTCT CTATGAGTGC TCGGCCACCA ACGTACTGGG CAGCATCACC 
AGCTCCTGTA CCGTGGCTGT GGCCCGAGTC CCAGGAAAGC TAGCTCCTCC AGAGGTACCC 
CAGACCTACC AGGACACGGC GCTGGTGCTG TGGAAGCCGG GAGACAGCCG GGCACCTTGC 
ACGTATACGC TGGAGCGGCG AGTGGATGGG GAGTCTGTGT GGCACCCTGT GAGCTCAGGC 
ATCCCCGACT GTTACTACAA CGTGACCCAC CTGCCAGTTG GCGTGACTGT GAGGTTCCGT 
GTGGCCTGTG CCAACCGTGC TGGGCAGGGG CCCTTCAGCA ACTCTTCTGA GAAGGTCTTT 
GTCAGGGGTA CTCAAGATTC TTCAGCTGTG CCATCTGCTG CCCACCAAGA GGCCCCTGTC 
ACCTCAAGGC CAGCCAGGGC CCGGCCTCCT GACTCTCCTA CCTCACTGGC CCCACCCCTA 
GCTCCTGCTG CCCCCACACC CCCGTCAGTC ACTGTCAGCC CCTCATCTCC CCCCACACCT 
CCTAGCCAGG CCTTGTCCTC GCTCAAGGCT GTGGGTCCAC CACCCCAAAC CCCTCCACGA 
AGACACAGGG GCCTGCAGGC TGCCCGGCCA GCGGAGCCCA CCCTACCCAG TACCCACGTC 
ACCCCAAGTG AGCCCAAGCC TTTCGTCCTT GACACTGGGA CCCCGATCCC AGCCTCCACT 
CCTCAAGGGG TTAAACCAGT GTCTTCCTCT ACTCCTGTGT ATGTGGTGAC TTCCTTTGTG 
TCTGCACCAC CAGCCCCTGA GCCCCCAGCC CCTGAGCCCC CTCCTGAGCC TACCAAGGTG 
ACTGTGCAGA GCCTCAGCCC GGCCAAGGAG GTGGTCAGCT CCCCTGGGAG CAGTCCCCGA 
AGCTCTCCCA GGCCTGAGGG TACCACTCTT CGACAGGGTC CCCCTCAGAA ACCCTACACC 
TTCCTGGAGG AGAAAGCCAG GGGCCGCTTT GGTGTTGTGC GAGCGTGCCG GGAGAATGCC 
ACGGGGCGAA CGTTCGTGGC CAAGATCGTG CCCTATGCTG CCGAGGGCAA GCGGCGGGTC 
CTGCAGGAGT ACGAGGTGCT GCGGACCCTG CACCACGAGC GGATCATGTC CCTGCACGAG 
GCCTACATCA CCCCTCGGTA CCTCGTGCTC ATTGCTGAGA GCTGTGGCAA CCGGGAACTC 
CTCTGTGGGC TCAGTGACAG GTTCCGGTAT TCTGAGGATG ACGTGGCCAC TTACATGGTG 
CAGCTGCTAC AAGGCCTGGA CTACCTCCAC GGCCACCACG TGCTCCACCT AGACATCAAG 
CCAGACAACC TGCTGCTGGC CCCTGACAAT GCCCTCAAGA TTGTGGACTT TGGCAGTGCC 
CAGCCCTACA ACCCCCAGGC CCTTAGGCCC CTTGGCCACC GCACGGGCAC GCTGGAGTTC 
ATGGCTCCGG AGATGGTGAA GGGAGAACCC ATCGGCTCTG CCACGGACAT CTGGGGAGCG 
GGTGTGCTCA CTTACATTAT GCTCAGTGGA CGCTCCCCGT TCTATGAGCC AGACCCCCAG 
GAAACGGAGG CTCGGATTGT GGGGGGCCGC TTTGATGCCT TCCAGCTGTA CCCCAATACA 
TCCCAGAGCG CCACCCTCTT CTTGCGAAAG GTTCTCTCTG TACATCCCTG GAGCCGGCCC 
TCCCTGCAGG ACTGCCTGGC CCACCCATGG TTGCAGGACG CCTACCTGAT GAAGCTGCGC 
CGCCAGACGC TCACCTTCAC CACCAACCGG CTCAAGGAGT TCCTGGGCGA GCAGCGGCGG 
CGCCGGGCTG AGGCTGCCAC CCGCCACAAG GTGCTGCTGC GCTCCTACCC TGGCGGCCCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005246299.1
CDS	126..7568
Translation	MGVLAMLLQGSIDLLPLLLEPAVGSLGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPTDVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARFDCKISGTPPPVVTWTHFGCPMEESENLRLRQDGGLHSLHIAHVGSEDEGLYAVSAVNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEQGELERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDVHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPQVVAVTGRMVTLTWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPGWAATGLRKGVQHIFRVLSTTVKSSSKPSPPSEPVQLLEHGPTLEEAPAMLDKPDIVYVVEGQPASVTVTFNHVEAQVVWRSCRGALLEARAGVYELSQPDDDQYCLRICRVSRRDMGALTCTARNRHGTQTCSVTLELAEAPRFESIMEDVEVGAGETARFAVVVEGKPLPDIMWYKDEVLLTESSHVSFVYEENECSLVVLSTGAQDGGVYTCTAQNLAGEVSCKAELAVHSAQTAMEVEGVGEDEDHRGRRLSDFYDIHQEIGRGAFSYLRRIVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHDCVLYFHEAFERRRGLVIVTELCTEELLERIARKPTVCESEIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTQAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGELRRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQPPAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPSEPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEAKFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAVGEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAESLGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPHISWMKDKKSLRSEPSVIIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARVPGKLAPPEVPQTYQDTALVLWKPGDSRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTHLPVGVTVRFRVACANRAGQGPFSNSSEKVFVRGTQDSSAVPSAAHQEAPVTSRPARARPPDSPTSLAPPLAPAAPTPPSVTVSPSSPPTPPSQALSSLKAVGPPPQTPPRRHRGLQAARPAEPTLPSTHVTPSEPKPFVLDTGTPIPASTPQGVKPVSSSTPVYVVTSFVSAPPAPEPPAPEPPPEPTKVTVQSLSPAKEVVSSPGSSPRSSPRPEGTTLRQGPPQKPYTFLEEKARGRFGVVRACRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERIMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYMVQLLQGLDYLHGHHVLHLDIKPDNLLLAPDNALKIVDFGSAQPYNPQALRPLGHRTGTLEFMAPEMVKGEPIGSATDIWGAGVLTYIMLSGRSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGGP

Target ORF information:

RefSeq Version	XM_005246242.4
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246242.4

ORF Insert Sequence:

ATGGGCGTCC TAGCCATGCT GCTCCAGGGT TCCATAGACC TGCTTCCCCT GCTTTTGGAG 
CCTGCAGTTG GTAGTTTAGG TGGGTCTACA TCCCCTTTCA GCAGCCCCAT CACCTCCGAC 
GAGGAATACC TGAGCCCCCC AGAGGAGTTC CCAGAGCCTG GGGAGACCTG GCCGCGAACC 
CCCACCATGA AGCCCAGTCC CAGCCAGAAC CGCCGTTCTT CTGACACTGG CTCCAAGGCA 
CCCCCCACCT TCAAGGTCTC ACTTATGGAC CAGTCAGTAA GAGAAGGCCA AGATGTCATC 
ATGAGCATCC GCGTGCAGGG GGAGCCCAAG CCTGTGGTCT CCTGGCTGAG AAACCGCCAG 
CCCGTGCGCC CAGACCAGCG GCGCTTTGCG GAGGAGGCTG AGGGTGGGCT GTGCCGGCTG 
CGGATCCTGG CTGCAGAGCG TGGCGATGCT GGTTTCTACA CTTGCAAAGC GGTCAATGAG 
TATGGTGCTC GGCAGTGCGA GGCCCGCTTG GAGGTCCGAG CACACCCTGA AAGCCGGTCC 
CTGGCCGTGC TGGCCCCCCT GCAGGACGTG GACGTGGGGG CCGGGGAGAT GGCGCTGTTT 
GAGTGCCTGG TGGCGGGGCC CACTGACGTG GAGGTGGATT GGCTGTGCCG TGGCCGCCTG 
CTGCAGCCTG CACTGCTCAA ATGCAAGATG CATTTCGATG GCCGCAAATG CAAGCTGCTA 
CTTACATCTG TACATGAGGA CGACAGTGGC GTCTACACCT GCAAGCTCAG CACGGCCAAA 
GATGAGCTGA CCTGCAGTGC CCGGCTGACC GTGCGGCCCT CGTTGGCACC CCTGTTCACA 
CGGCTGCTGG AAGATGTGGA GGTGTTGGAG GGCCGAGCTG CCCGTTTCGA CTGCAAGATC 
AGTGGCACCC CGCCCCCTGT TGTTACCTGG ACTCATTTTG GCTGCCCCAT GGAGGAGAGT 
GAGAACTTGC GGCTGCGGCA GGACGGGGGT CTGCACTCAC TGCACATTGC CCATGTGGGC 
AGCGAGGACG AGGGGCTCTA TGCGGTCAGT GCTGTTAACA CCCATGGCCA GGCCCACTGC 
TCAGCCCAGC TGTATGTAGA AGAGCCCCGG ACAGCCGCCT CAGGCCCCAG CTCGAAGCTG 
GAGAAGATGC CATCCATTCC CGAGGAGCCA GAGCAGGGTG AGCTGGAGCG GCTGTCCATT 
CCTGACTTCC TGCGGCCACT GCAGGACCTG GAGGTGGGAC TGGCCAAGGA GGCCATGCTA 
GAGTGCCAGG TGACCGGCCT GCCCTACCCC ACCATCAGCT GGTTCCACAA TGGCCACCGC 
ATCCAGAGCA GCGACGACCG GCGCATGACA CAGTACAGGG ATGTCCATCG CTTGGTGTTC 
CCTGCCGTGG GGCCTCAGCA CGCCGGTGTC TACAAGAGCG TCATTGCCAA CAAGCTGGGC 
AAAGCTGCCT GCTATGCCCA CCTGTATGTC ACAGATGTGG TCCCAGGCCC TCCAGATGGC 
GCCCCGCAGG TGGTGGCTGT GACGGGGAGG ATGGTCACAC TCACATGGAA CCCCCCCAGG 
AGTCTGGACA TGGCCATCGA CCCGGACTCC CTGACGTACA CAGTGCAGCA CCAGGTGCTG 
GGCTCGGACC AGTGGACGGC ACTGGTCACA GGCCTGCGGG AGCCAGGGTG GGCAGCCACA 
GGGCTGCGTA AGGGGGTCCA GCACATCTTC CGGGTCCTCA GCACCACTGT CAAGAGCAGC 
AGCAAGCCCT CACCCCCTTC TGAGCCTGTG CAGCTGCTGG AGCACGGCCC AACCCTGGAG 
GAGGCCCCTG CCATGCTGGA CAAACCAGAC ATCGTGTATG TGGTGGAGGG ACAGCCTGCC 
AGCGTCACCG TCACATTCAA CCATGTGGAG GCCCAGGTCG TCTGGAGGAG CTGCCGAGGG 
GCCCTCCTAG AGGCACGGGC CGGTGTGTAC GAGCTGAGCC AGCCAGATGA TGACCAGTAC 
TGTCTTCGGA TCTGCCGGGT GAGCCGCCGG GACATGGGGG CCCTCACCTG CACCGCCCGA 
AACCGTCACG GCACACAGAC CTGCTCGGTC ACATTGGAGC TGGCAGAGGC CCCTCGGTTT 
GAGTCCATCA TGGAGGACGT GGAGGTGGGG GCTGGGGAAA CTGCTCGCTT TGCGGTGGTG 
GTCGAGGGAA AACCACTGCC GGACATCATG TGGTACAAGG ACGAGGTGCT GCTGACCGAG 
AGCAGCCATG TGAGCTTCGT GTACGAGGAG AATGAGTGCT CCCTGGTGGT GCTCAGCACG 
GGGGCCCAGG ATGGAGGCGT CTACACCTGC ACCGCCCAGA ACCTGGCGGG TGAGGTCTCC 
TGCAAAGCAG AGTTGGCTGT GCATTCAGCT CAGACAGCTA TGGAGGTCGA GGGGGTCGGG 
GAGGATGAGG ACCATCGAGG AAGGAGACTC AGCGACTTTT ATGACATCCA CCAGGAGATC 
GGCAGGGGTG CTTTCTCCTA CTTGCGGCGC ATAGTGGAGC GTAGCTCCGG CCTGGAGTTT 
GCGGCCAAGT TCATCCCCAG CCAGGCCAAG CCAAAGGCAT CAGCGCGTCG GGAGGCCCGG 
CTGCTGGCCA GGCTCCAGCA CGACTGTGTC CTCTACTTCC ATGAGGCCTT CGAGAGGCGC 
CGGGGACTGG TCATTGTCAC CGAGCTCTGC ACAGAGGAGC TGCTGGAGCG AATCGCCAGG 
AAACCCACCG TGTGTGAGTC TGAGATCCGG GCCTATATGC GGCAGGTGCT AGAGGGAATA 
CACTACCTGC ACCAGAGCCA CGTGCTGCAC CTCGATGTCA AGCCTGAGAA CCTGCTGGTG 
TGGGATGGTG CTGCGGGCGA GCAGCAGGTG CGGATCTGTG ACTTTGGGAA TGCCCAGGAG 
CTGACTCCAG GAGAGCCCCA GTACTGCCAG TATGGCACAC CTGAGTTTGT AGCACCCGAG 
ATTGTCAATC AGAGCCCCGT GTCTGGAGTC ACTGACATCT GGCCTGTGGG TGTTGTTGCC 
TTCCTCTGTC TGACAGGAAT CTCCCCGTTT GTTGGGGAAA ATGACCGGAC AACATTGATG 
AACATCCGAA ACTACAACGT GGCCTTCGAG GAGACCACAT TCCTGAGCCT GAGCAGGGAG 
GCCCGGGGCT TCCTCATCAA AGTGTTGGTG CAGGACCGGC TGAGACCTAC CGCAGAAGAG 
ACCCTAGAAC ATCCTTGGTT CAAAACTCAG GCAAAGGGCG CAGAGGTGAG CACGGATCAC 
CTGAAGCTAT TCCTCTCCCG GCGGAGGTGG CAGCGCTCCC AGATCAGCTA CAAATGCCAC 
CTGGTGCTGC GCCCCATCCC CGAGCTGCTG CGGGCCCCCC CAGAGCGGGT GTGGGTGACC 
ATGCCCAGAA GGCCACCCCC CAGTGGGGGG CTCTCATCCT CCTCGGATTC TGAAGAGGAA 
GAGCTGGAAG AGCTGCCCTC AGTGCCCCGC CCACTGCAGC CCGAGTTCTC TGGCTCCCGG 
GTGTCCCTCA CAGACATTCC CACTGAGGAT GAGGCCCTGG GGACCCCAGA GACTGGGGCT 
GCCACCCCCA TGGACTGGCA GGAGCAGGGA AGGGCTCCCT CTCAGGACCA GGAGGCTCCC 
AGCCCAGAGG CCCTCCCCTC CCCAGGCCAG GAGCCCGCAG CTGGGGCTAG CCCCAGGCGG 
GGAGAGCTCC GCAGGGGCAG CTCGGCTGAG AGCGCCCTGC CCCGGGCCGG GCCGCGGGAG 
CTGGGCCGGG GCCTGCACAA GGCGGCGTCT GTGGAGCTGC CGCAGCGCCG GAGCCCCAGC 
CCGGGAGCCA CCCGCCTGGC CCGGGGAGGC CTGGGTGAGG GCGAGTATGC CCAGAGGCTG 
CAGGCCCTGC GCCAGCGGCT GCTGCGGGGA GGCCCCGAGG ATGGCAAGGT CAGCGGCCTC 
AGGGGTCCCC TGCTGGAGAG CCTGGGGGGC CGTGCTCGGG ACCCCCGGAT GGCACGAGCT 
GCCTCCAGCG AGGCAGCGCC CCACCACCAG CCCCCACTCG AGAACCGGGG CCTGCAAAAG 
AGCAGCAGCT TCTCCCAGGG TGAGGCGGAG CCCCGGGGCC GGCACCGCCG AGCGGGGGCG 
CCCCTCGAGA TCCCCGTGGC CAGGCTTGGG GCCCGTAGGC TACAGGAGTC TCCTTCCCTG 
TCTGCCCTCA GCGAGGCCCA GCCATCCAGC CCTGCACGGC CCAGCGCCCC CAAACCCAGT 
ACCCCTAAGT CTGCAGAACC TTCTGCCACC ACACCTAGTG ATGCTCCGCA GCCCCCCGCA 
CCCCAGCCTG CCCAAGACAA GGCTCCAGAG CCCAGGCCAG AACCAGTCCG AGCCTCCAAG 
CCTGCACCAC CCCCCCAGGC CCTGCAAACC CTAGCGCTGC CCCTCACACC CTATGCTCAG 
ATCATTCAGT CCCTCCAGCT GTCAGGCCAC GCCCAGGGCC CCTCGCAGGG CCCTGCCGCG 
CCGCCTTCAG AGCCCAAGCC CCACGCTGCT GTCTTTGCCA GGGTGGCCTC CCCACCTCCG 
GGAGCCCCCG AGAAGCGCGT GCCCTCAGCC GGGGGTCCCC CGGTGCTAGC CGAGAAAGCC 
CGAGTTCCCA CGGTGCCCCC CAGGCCAGGC AGCAGTCTCA GTAGCAGCAT CGAAAACTTG 
GAGTCGGAGG CCGTGTTCGA GGCCAAGTTC AAGCGCAGCC GCGAGTCGCC CCTGTCGCTG 
GGGCTGCGGC TGCTGAGCCG TTCGCGCTCG GAGGAGCGCG GCCCCTTCCG TGGGGCCGAG 
GAGGAGGATG GCATATACCG GCCCAGCCCG GCGGGGACCC CGCTGGAGCT GGTGCGACGG 
CCTGAGCGCT CACGCTCGGT GCAGGACCTC AGGGCTGTCG GAGAGCCTGG CCTCGTCCGC 
CGCCTCTCGC TGTCACTGTC CCAGCGGCTG CGGCGGACCC CTCCCGCGCA GCGCCACCCG 
GCCTGGGAGG CCCGCGGCGG GGACGGAGAG AGCTCGGAGG GCGGGAGCTC GGCGCGGGGC 
TCCCCGGTGC TGGCGATGCG CAGGCGGCTG AGCTTCACCC TGGAGCGGCT GTCCAGCCGA 
TTGCAGCGCA GTGGCAGCAG CGAGGACTCG GGGGGCGCGT CGGGCCGCAG CACGCCGCTG 
TTCGGACGGC TTCGCAGGGC CACGTCCGAG GGCGAGAGTC TGCGGCGCCT TGGCCTTCCG 
CACAACCAGT TGGCCGCCCA GGCCGGCGCC ACCACGCCTT CCGCCGAGTC CCTGGGCTCC 
GAGGCCAGCG CCACGTCGGG CTCCTCAGCC CCAGGGGAAA GCCGAAGCCG GCTCCGCTGG 
GGCTTCTCTC GGCCGCGGAA GGACAAGGGG TTATCGCCAC CAAACCTCTC TGCCAGCGTC 
CAGGAGGAGT TGGGTCACCA GTACGTGCGC AGTGAGTCAG ACTTCCCCCC AGTCTTCCAC 
ATCAAACTCA AGGACCAGGT GCTGCTGGAG GGGGAGGCAG CCACCCTGCT CTGCCTGCCA 
GCGGCCTGCC CTGCACCGCA CATCTCCTGG ATGAAAGACA AGAAGTCCTT GAGGTCAGAG 
CCCTCAGTGA TCATCGTGTC CTGCAAAGAT GGGCGGCAGC TGCTCAGCAT CCCCCGGGCG 
GGCAAGCGGC ACGCCGGTCT CTATGAGTGC TCGGCCACCA ACGTACTGGG CAGCATCACC 
AGCTCCTGTA CCGTGGCTGT GGCCCGAGTC CCAGGAAAGC TAGCTCCTCC AGAGGTACCC 
CAGACCTACC AGGACACGGC GCTGGTGCTG TGGAAGCCGG GAGACAGCCG GGCACCTTGC 
ACGTATACGC TGGAGCGGCG AGTGGATGGG GAGTCTGTGT GGCACCCTGT GAGCTCAGGC 
ATCCCCGACT GTTACTACAA CGTGACCCAC CTGCCAGTTG GCGTGACTGT GAGGTTCCGT 
GTGGCCTGTG CCAACCGTGC TGGGCAGGGG CCCTTCAGCA ACTCTTCTGA GAAGGTCTTT 
GTCAGGGGTA CTCAAGATTC TTCAGCTGTG CCATCTGCTG CCCACCAAGA GGCCCCTGTC 
ACCTCAAGGC CAGCCAGGGC CCGGCCTCCT GACTCTCCTA CCTCACTGGC CCCACCCCTA 
GCTCCTGCTG CCCCCACACC CCCGTCAGTC ACTGTCAGCC CCTCATCTCC CCCCACACCT 
CCTAGCCAGG CCTTGTCCTC GCTCAAGGCT GTGGGTCCAC CACCCCAAAC CCCTCCACGA 
AGACACAGGG GCCTGCAGGC TGCCCGGCCA GCGGAGCCCA CCCTACCCAG TACCCACGTC 
ACCCCAAGTG AGCCCAAGCC TTTCGTCCTT GACACTGGGA CCCCGATCCC AGCCTCCACT 
CCTCAAGGGG TTAAACCAGT GTCTTCCTCT ACTCCTGTGT ATGTGGTGAC TTCCTTTGTG 
TCTGCACCAC CAGCCCCTGA GCCCCCAGCC CCTGAGCCCC CTCCTGAGCC TACCAAGGTG 
ACTGTGCAGA GCCTCAGCCC GGCCAAGGAG GTGGTCAGCT CCCCTGGGAG CAGTCCCCGA 
AGCTCTCCCA GGCCTGAGGG TACCACTCTT CGACAGGGTC CCCCTCAGAA ACCCTACACC 
TTCCTGGAGG AGAAAGCCAG GGGCCGCTTT GGTGTTGTGC GAGCGTGCCG GGAGAATGCC 
ACGGGGCGAA CGTTCGTGGC CAAGATCGTG CCCTATGCTG CCGAGGGCAA GCGGCGGGTC 
CTGCAGGAGT ACGAGGTGCT GCGGACCCTG CACCACGAGC GGATCATGTC CCTGCACGAG 
GCCTACATCA CCCCTCGGTA CCTCGTGCTC ATTGCTGAGA GCTGTGGCAA CCGGGAACTC 
CTCTGTGGGC TCAGTGACAG GTTCCGGTAT TCTGAGGATG ACGTGGCCAC TTACATGGTG 
CAGCTGCTAC AAGGCCTGGA CTACCTCCAC GGCCACCACG TGCTCCACCT AGACATCAAG 
CCAGACAACC TGCTGCTGGC CCCTGACAAT GCCCTCAAGA TTGTGGACTT TGGCAGTGCC 
CAGCCCTACA ACCCCCAGGC CCTTAGGCCC CTTGGCCACC GCACGGGCAC GCTGGAGTTC 
ATGGCTCCGG AGATGGTGAA GGGAGAACCC ATCGGCTCTG CCACGGACAT CTGGGGAGCG 
GGTGTGCTCA CTTACATTAT GCTCAGTGGA CGCTCCCCGT TCTATGAGCC AGACCCCCAG 
GAAACGGAGG CTCGGATTGT GGGGGGCCGC TTTGATGCCT TCCAGCTGTA CCCCAATACA 
TCCCAGAGCG CCACCCTCTT CTTGCGAAAG GTTCTCTCTG TACATCCCTG GAGCCGGCCC 
TCCCTGCAGG ACTGCCTGGC CCACCCATGG TTGCAGGACG CCTACCTGAT GAAGCTGCGC 
CGCCAGACGC TCACCTTCAC CACCAACCGG CTCAAGGAGT TCCTGGGCGA GCAGCGGCGG 
CGCCGGGCTG AGGCTGCCAC CCGCCACAAG GTGCTGCTGC GCTCCTACCC TGGCGGCCCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_017003158.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858647.1 Homo sapiens SPEG complex locus (SPEG), transcript variant X4, mRNA.

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CloneID	OHu35758
Clone ID Related Accession (Same CDS sequence)	XM_017003158.1 , XM_005246241.1 , XM_006712193.3 , XM_017003158.2 , XM_005246240.2
Accession Version	XM_017003158.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCCA GTCCCAGCCA GAACCGCCGT TCTTCTGACA CTGGCTCCAA GGCACCCCCC 
ACCTTCAAGG TCTCACTTAT GGACCAGTCA GTAAGAGAAG GCCAAGATGT CATCATGAGC 
ATCCGCGTGC AGGGGGAGCC CAAGCCTGTG GTCTCCTGGC TGAGAAACCG CCAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCGGAGGAG GCTGAGGGTG GGCTGTGCCG GCTGCGGATC 
CTGGCTGCAG AGCGTGGCGA TGCTGGTTTC TACACTTGCA AAGCGGTCAA TGAGTATGGT 
GCTCGGCAGT GCGAGGCCCG CTTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCGG GGCCCACTGA CGTGGAGGTG GATTGGCTGT GCCGTGGCCG CCTGCTGCAG 
CCTGCACTGC TCAAATGCAA GATGCATTTC GATGGCCGCA AATGCAAGCT GCTACTTACA 
TCTGTACATG AGGACGACAG TGGCGTCTAC ACCTGCAAGC TCAGCACGGC CAAAGATGAG 
CTGACCTGCA GTGCCCGGCT GACCGTGCGG CCCTCGTTGG CACCCCTGTT CACACGGCTG 
CTGGAAGATG TGGAGGTGTT GGAGGGCCGA GCTGCCCGTT TCGACTGCAA GATCAGTGGC 
ACCCCGCCCC CTGTTGTTAC CTGGACTCAT TTTGGCTGCC CCATGGAGGA GAGTGAGAAC 
TTGCGGCTGC GGCAGGACGG GGGTCTGCAC TCACTGCACA TTGCCCATGT GGGCAGCGAG 
GACGAGGGGC TCTATGCGGT CAGTGCTGTT AACACCCATG GCCAGGCCCA CTGCTCAGCC 
CAGCTGTATG TAGAAGAGCC CCGGACAGCC GCCTCAGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCCA TTCCCGAGGA GCCAGAGCAG GGTGAGCTGG AGCGGCTGTC CATTCCTGAC 
TTCCTGCGGC CACTGCAGGA CCTGGAGGTG GGACTGGCCA AGGAGGCCAT GCTAGAGTGC 
CAGGTGACCG GCCTGCCCTA CCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATCCAG 
AGCAGCGACG ACCGGCGCAT GACACAGTAC AGGGATGTCC ATCGCTTGGT GTTCCCTGCC 
GTGGGGCCTC AGCACGCCGG TGTCTACAAG AGCGTCATTG CCAACAAGCT GGGCAAAGCT 
GCCTGCTATG CCCACCTGTA TGTCACAGAT GTGGTCCCAG GCCCTCCAGA TGGCGCCCCG 
CAGGTGGTGG CTGTGACGGG GAGGATGGTC ACACTCACAT GGAACCCCCC CAGGAGTCTG 
GACATGGCCA TCGACCCGGA CTCCCTGACG TACACAGTGC AGCACCAGGT GCTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCAG GGTGGGCAGC CACAGGGCTG 
CGTAAGGGGG TCCAGCACAT CTTCCGGGTC CTCAGCACCA CTGTCAAGAG CAGCAGCAAG 
CCCTCACCCC CTTCTGAGCC TGTGCAGCTG CTGGAGCACG GCCCAACCCT GGAGGAGGCC 
CCTGCCATGC TGGACAAACC AGACATCGTG TATGTGGTGG AGGGACAGCC TGCCAGCGTC 
ACCGTCACAT TCAACCATGT GGAGGCCCAG GTCGTCTGGA GGAGCTGCCG AGGGGCCCTC 
CTAGAGGCAC GGGCCGGTGT GTACGAGCTG AGCCAGCCAG ATGATGACCA GTACTGTCTT 
CGGATCTGCC GGGTGAGCCG CCGGGACATG GGGGCCCTCA CCTGCACCGC CCGAAACCGT 
CACGGCACAC AGACCTGCTC GGTCACATTG GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC 
ATCATGGAGG ACGTGGAGGT GGGGGCTGGG GAAACTGCTC GCTTTGCGGT GGTGGTCGAG 
GGAAAACCAC TGCCGGACAT CATGTGGTAC AAGGACGAGG TGCTGCTGAC CGAGAGCAGC 
CATGTGAGCT TCGTGTACGA GGAGAATGAG TGCTCCCTGG TGGTGCTCAG CACGGGGGCC 
CAGGATGGAG GCGTCTACAC CTGCACCGCC CAGAACCTGG CGGGTGAGGT CTCCTGCAAA 
GCAGAGTTGG CTGTGCATTC AGCTCAGACA GCTATGGAGG TCGAGGGGGT CGGGGAGGAT 
GAGGACCATC GAGGAAGGAG ACTCAGCGAC TTTTATGACA TCCACCAGGA GATCGGCAGG 
GGTGCTTTCT CCTACTTGCG GCGCATAGTG GAGCGTAGCT CCGGCCTGGA GTTTGCGGCC 
AAGTTCATCC CCAGCCAGGC CAAGCCAAAG GCATCAGCGC GTCGGGAGGC CCGGCTGCTG 
GCCAGGCTCC AGCACGACTG TGTCCTCTAC TTCCATGAGG CCTTCGAGAG GCGCCGGGGA 
CTGGTCATTG TCACCGAGCT CTGCACAGAG GAGCTGCTGG AGCGAATCGC CAGGAAACCC 
ACCGTGTGTG AGTCTGAGAT CCGGGCCTAT ATGCGGCAGG TGCTAGAGGG AATACACTAC 
CTGCACCAGA GCCACGTGCT GCACCTCGAT GTCAAGCCTG AGAACCTGCT GGTGTGGGAT 
GGTGCTGCGG GCGAGCAGCA GGTGCGGATC TGTGACTTTG GGAATGCCCA GGAGCTGACT 
CCAGGAGAGC CCCAGTACTG CCAGTATGGC ACACCTGAGT TTGTAGCACC CGAGATTGTC 
AATCAGAGCC CCGTGTCTGG AGTCACTGAC ATCTGGCCTG TGGGTGTTGT TGCCTTCCTC 
TGTCTGACAG GAATCTCCCC GTTTGTTGGG GAAAATGACC GGACAACATT GATGAACATC 
CGAAACTACA ACGTGGCCTT CGAGGAGACC ACATTCCTGA GCCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGTT GGTGCAGGAC CGGCTGAGAC CTACCGCAGA AGAGACCCTA 
GAACATCCTT GGTTCAAAAC TCAGGCAAAG GGCGCAGAGG TGAGCACGGA TCACCTGAAG 
CTATTCCTCT CCCGGCGGAG GTGGCAGCGC TCCCAGATCA GCTACAAATG CCACCTGGTG 
CTGCGCCCCA TCCCCGAGCT GCTGCGGGCC CCCCCAGAGC GGGTGTGGGT GACCATGCCC 
AGAAGGCCAC CCCCCAGTGG GGGGCTCTCA TCCTCCTCGG ATTCTGAAGA GGAAGAGCTG 
GAAGAGCTGC CCTCAGTGCC CCGCCCACTG CAGCCCGAGT TCTCTGGCTC CCGGGTGTCC 
CTCACAGACA TTCCCACTGA GGATGAGGCC CTGGGGACCC CAGAGACTGG GGCTGCCACC 
CCCATGGACT GGCAGGAGCA GGGAAGGGCT CCCTCTCAGG ACCAGGAGGC TCCCAGCCCA 
GAGGCCCTCC CCTCCCCAGG CCAGGAGCCC GCAGCTGGGG CTAGCCCCAG GCGGGGAGAG 
CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC CTGCCCCGGG CCGGGCCGCG GGAGCTGGGC 
CGGGGCCTGC ACAAGGCGGC GTCTGTGGAG CTGCCGCAGC GCCGGAGCCC CAGCCCGGGA 
GCCACCCGCC TGGCCCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGCCAGC GGCTGCTGCG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGT 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCT CGGGACCCCC GGATGGCACG AGCTGCCTCC 
AGCGAGGCAG CGCCCCACCA CCAGCCCCCA CTCGAGAACC GGGGCCTGCA AAAGAGCAGC 
AGCTTCTCCC AGGGTGAGGC GGAGCCCCGG GGCCGGCACC GCCGAGCGGG GGCGCCCCTC 
GAGATCCCCG TGGCCAGGCT TGGGGCCCGT AGGCTACAGG AGTCTCCTTC CCTGTCTGCC 
CTCAGCGAGG CCCAGCCATC CAGCCCTGCA CGGCCCAGCG CCCCCAAACC CAGTACCCCT 
AAGTCTGCAG AACCTTCTGC CACCACACCT AGTGATGCTC CGCAGCCCCC CGCACCCCAG 
CCTGCCCAAG ACAAGGCTCC AGAGCCCAGG CCAGAACCAG TCCGAGCCTC CAAGCCTGCA 
CCACCCCCCC AGGCCCTGCA AACCCTAGCG CTGCCCCTCA CACCCTATGC TCAGATCATT 
CAGTCCCTCC AGCTGTCAGG CCACGCCCAG GGCCCCTCGC AGGGCCCTGC CGCGCCGCCT 
TCAGAGCCCA AGCCCCACGC TGCTGTCTTT GCCAGGGTGG CCTCCCCACC TCCGGGAGCC 
CCCGAGAAGC GCGTGCCCTC AGCCGGGGGT CCCCCGGTGC TAGCCGAGAA AGCCCGAGTT 
CCCACGGTGC CCCCCAGGCC AGGCAGCAGT CTCAGTAGCA GCATCGAAAA CTTGGAGTCG 
GAGGCCGTGT TCGAGGCCAA GTTCAAGCGC AGCCGCGAGT CGCCCCTGTC GCTGGGGCTG 
CGGCTGCTGA GCCGTTCGCG CTCGGAGGAG CGCGGCCCCT TCCGTGGGGC CGAGGAGGAG 
GATGGCATAT ACCGGCCCAG CCCGGCGGGG ACCCCGCTGG AGCTGGTGCG ACGGCCTGAG 
CGCTCACGCT CGGTGCAGGA CCTCAGGGCT GTCGGAGAGC CTGGCCTCGT CCGCCGCCTC 
TCGCTGTCAC TGTCCCAGCG GCTGCGGCGG ACCCCTCCCG CGCAGCGCCA CCCGGCCTGG 
GAGGCCCGCG GCGGGGACGG AGAGAGCTCG GAGGGCGGGA GCTCGGCGCG GGGCTCCCCG 
GTGCTGGCGA TGCGCAGGCG GCTGAGCTTC ACCCTGGAGC GGCTGTCCAG CCGATTGCAG 
CGCAGTGGCA GCAGCGAGGA CTCGGGGGGC GCGTCGGGCC GCAGCACGCC GCTGTTCGGA 
CGGCTTCGCA GGGCCACGTC CGAGGGCGAG AGTCTGCGGC GCCTTGGCCT TCCGCACAAC 
CAGTTGGCCG CCCAGGCCGG CGCCACCACG CCTTCCGCCG AGTCCCTGGG CTCCGAGGCC 
AGCGCCACGT CGGGCTCCTC AGCCCCAGGG GAAAGCCGAA GCCGGCTCCG CTGGGGCTTC 
TCTCGGCCGC GGAAGGACAA GGGGTTATCG CCACCAAACC TCTCTGCCAG CGTCCAGGAG 
GAGTTGGGTC ACCAGTACGT GCGCAGTGAG TCAGACTTCC CCCCAGTCTT CCACATCAAA 
CTCAAGGACC AGGTGCTGCT GGAGGGGGAG GCAGCCACCC TGCTCTGCCT GCCAGCGGCC 
TGCCCTGCAC CGCACATCTC CTGGATGAAA GACAAGAAGT CCTTGAGGTC AGAGCCCTCA 
GTGATCATCG TGTCCTGCAA AGATGGGCGG CAGCTGCTCA GCATCCCCCG GGCGGGCAAG 
CGGCACGCCG GTCTCTATGA GTGCTCGGCC ACCAACGTAC TGGGCAGCAT CACCAGCTCC 
TGTACCGTGG CTGTGGCCCG AGTCCCAGGA AAGCTAGCTC CTCCAGAGGT ACCCCAGACC 
TACCAGGACA CGGCGCTGGT GCTGTGGAAG CCGGGAGACA GCCGGGCACC TTGCACGTAT 
ACGCTGGAGC GGCGAGTGGA TGGGGAGTCT GTGTGGCACC CTGTGAGCTC AGGCATCCCC 
GACTGTTACT ACAACGTGAC CCACCTGCCA GTTGGCGTGA CTGTGAGGTT CCGTGTGGCC 
TGTGCCAACC GTGCTGGGCA GGGGCCCTTC AGCAACTCTT CTGAGAAGGT CTTTGTCAGG 
GGTACTCAAG ATTCTTCAGC TGTGCCATCT GCTGCCCACC AAGAGGCCCC TGTCACCTCA 
AGGCCAGCCA GGGCCCGGCC TCCTGACTCT CCTACCTCAC TGGCCCCACC CCTAGCTCCT 
GCTGCCCCCA CACCCCCGTC AGTCACTGTC AGCCCCTCAT CTCCCCCCAC ACCTCCTAGC 
CAGGCCTTGT CCTCGCTCAA GGCTGTGGGT CCACCACCCC AAACCCCTCC ACGAAGACAC 
AGGGGCCTGC AGGCTGCCCG GCCAGCGGAG CCCACCCTAC CCAGTACCCA CGTCACCCCA 
AGTGAGCCCA AGCCTTTCGT CCTTGACACT GGGACCCCGA TCCCAGCCTC CACTCCTCAA 
GGGGTTAAAC CAGTGTCTTC CTCTACTCCT GTGTATGTGG TGACTTCCTT TGTGTCTGCA 
CCACCAGCCC CTGAGCCCCC AGCCCCTGAG CCCCCTCCTG AGCCTACCAA GGTGACTGTG 
CAGAGCCTCA GCCCGGCCAA GGAGGTGGTC AGCTCCCCTG GGAGCAGTCC CCGAAGCTCT 
CCCAGGCCTG AGGGTACCAC TCTTCGACAG GGTCCCCCTC AGAAACCCTA CACCTTCCTG 
GAGGAGAAAG CCAGGGGCCG CTTTGGTGTT GTGCGAGCGT GCCGGGAGAA TGCCACGGGG 
CGAACGTTCG TGGCCAAGAT CGTGCCCTAT GCTGCCGAGG GCAAGCGGCG GGTCCTGCAG 
GAGTACGAGG TGCTGCGGAC CCTGCACCAC GAGCGGATCA TGTCCCTGCA CGAGGCCTAC 
ATCACCCCTC GGTACCTCGT GCTCATTGCT GAGAGCTGTG GCAACCGGGA ACTCCTCTGT 
GGGCTCAGTG ACAGGTTCCG GTATTCTGAG GATGACGTGG CCACTTACAT GGTGCAGCTG 
CTACAAGGCC TGGACTACCT CCACGGCCAC CACGTGCTCC ACCTAGACAT CAAGCCAGAC 
AACCTGCTGC TGGCCCCTGA CAATGCCCTC AAGATTGTGG ACTTTGGCAG TGCCCAGCCC 
TACAACCCCC AGGCCCTTAG GCCCCTTGGC CACCGCACGG GCACGCTGGA GTTCATGGCT 
CCGGAGATGG TGAAGGGAGA ACCCATCGGC TCTGCCACGG ACATCTGGGG AGCGGGTGTG 
CTCACTTACA TTATGCTCAG TGGACGCTCC CCGTTCTATG AGCCAGACCC CCAGGAAACG 
GAGGCTCGGA TTGTGGGGGG CCGCTTTGAT GCCTTCCAGC TGTACCCCAA TACATCCCAG 
AGCGCCACCC TCTTCTTGCG AAAGGTTCTC TCTGTACATC CCTGGAGCCG GCCCTCCCTG 
CAGGACTGCC TGGCCCACCC ATGGTTGCAG GACGCCTACC TGATGAAGCT GCGCCGCCAG 
ACGCTCACCT TCACCACCAA CCGGCTCAAG GAGTTCCTGG GCGAGCAGCG GCGGCGCCGG 
GCTGAGGCTG CCACCCGCCA CAAGGTGCTG CTGCGCTCCT ACCCTGGCGG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858647.1
CDS	1887..9143
Translation	MKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPTDVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARFDCKISGTPPPVVTWTHFGCPMEESENLRLRQDGGLHSLHIAHVGSEDEGLYAVSAVNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEQGELERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDVHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPQVVAVTGRMVTLTWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPGWAATGLRKGVQHIFRVLSTTVKSSSKPSPPSEPVQLLEHGPTLEEAPAMLDKPDIVYVVEGQPASVTVTFNHVEAQVVWRSCRGALLEARAGVYELSQPDDDQYCLRICRVSRRDMGALTCTARNRHGTQTCSVTLELAEAPRFESIMEDVEVGAGETARFAVVVEGKPLPDIMWYKDEVLLTESSHVSFVYEENECSLVVLSTGAQDGGVYTCTAQNLAGEVSCKAELAVHSAQTAMEVEGVGEDEDHRGRRLSDFYDIHQEIGRGAFSYLRRIVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHDCVLYFHEAFERRRGLVIVTELCTEELLERIARKPTVCESEIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTQAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGELRRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQPPAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPSEPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEAKFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAVGEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAESLGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPHISWMKDKKSLRSEPSVIIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARVPGKLAPPEVPQTYQDTALVLWKPGDSRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTHLPVGVTVRFRVACANRAGQGPFSNSSEKVFVRGTQDSSAVPSAAHQEAPVTSRPARARPPDSPTSLAPPLAPAAPTPPSVTVSPSSPPTPPSQALSSLKAVGPPPQTPPRRHRGLQAARPAEPTLPSTHVTPSEPKPFVLDTGTPIPASTPQGVKPVSSSTPVYVVTSFVSAPPAPEPPAPEPPPEPTKVTVQSLSPAKEVVSSPGSSPRSSPRPEGTTLRQGPPQKPYTFLEEKARGRFGVVRACRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERIMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYMVQLLQGLDYLHGHHVLHLDIKPDNLLLAPDNALKIVDFGSAQPYNPQALRPLGHRTGTLEFMAPEMVKGEPIGSATDIWGAGVLTYIMLSGRSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGGP

Target ORF information:

RefSeq Version	XM_017003158.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SPEG complex locus (SPEG), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003158.1

ORF Insert Sequence:

ATGAAGCCCA GTCCCAGCCA GAACCGCCGT TCTTCTGACA CTGGCTCCAA GGCACCCCCC 
ACCTTCAAGG TCTCACTTAT GGACCAGTCA GTAAGAGAAG GCCAAGATGT CATCATGAGC 
ATCCGCGTGC AGGGGGAGCC CAAGCCTGTG GTCTCCTGGC TGAGAAACCG CCAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCGGAGGAG GCTGAGGGTG GGCTGTGCCG GCTGCGGATC 
CTGGCTGCAG AGCGTGGCGA TGCTGGTTTC TACACTTGCA AAGCGGTCAA TGAGTATGGT 
GCTCGGCAGT GCGAGGCCCG CTTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCGG GGCCCACTGA CGTGGAGGTG GATTGGCTGT GCCGTGGCCG CCTGCTGCAG 
CCTGCACTGC TCAAATGCAA GATGCATTTC GATGGCCGCA AATGCAAGCT GCTACTTACA 
TCTGTACATG AGGACGACAG TGGCGTCTAC ACCTGCAAGC TCAGCACGGC CAAAGATGAG 
CTGACCTGCA GTGCCCGGCT GACCGTGCGG CCCTCGTTGG CACCCCTGTT CACACGGCTG 
CTGGAAGATG TGGAGGTGTT GGAGGGCCGA GCTGCCCGTT TCGACTGCAA GATCAGTGGC 
ACCCCGCCCC CTGTTGTTAC CTGGACTCAT TTTGGCTGCC CCATGGAGGA GAGTGAGAAC 
TTGCGGCTGC GGCAGGACGG GGGTCTGCAC TCACTGCACA TTGCCCATGT GGGCAGCGAG 
GACGAGGGGC TCTATGCGGT CAGTGCTGTT AACACCCATG GCCAGGCCCA CTGCTCAGCC 
CAGCTGTATG TAGAAGAGCC CCGGACAGCC GCCTCAGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCCA TTCCCGAGGA GCCAGAGCAG GGTGAGCTGG AGCGGCTGTC CATTCCTGAC 
TTCCTGCGGC CACTGCAGGA CCTGGAGGTG GGACTGGCCA AGGAGGCCAT GCTAGAGTGC 
CAGGTGACCG GCCTGCCCTA CCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATCCAG 
AGCAGCGACG ACCGGCGCAT GACACAGTAC AGGGATGTCC ATCGCTTGGT GTTCCCTGCC 
GTGGGGCCTC AGCACGCCGG TGTCTACAAG AGCGTCATTG CCAACAAGCT GGGCAAAGCT 
GCCTGCTATG CCCACCTGTA TGTCACAGAT GTGGTCCCAG GCCCTCCAGA TGGCGCCCCG 
CAGGTGGTGG CTGTGACGGG GAGGATGGTC ACACTCACAT GGAACCCCCC CAGGAGTCTG 
GACATGGCCA TCGACCCGGA CTCCCTGACG TACACAGTGC AGCACCAGGT GCTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCAG GGTGGGCAGC CACAGGGCTG 
CGTAAGGGGG TCCAGCACAT CTTCCGGGTC CTCAGCACCA CTGTCAAGAG CAGCAGCAAG 
CCCTCACCCC CTTCTGAGCC TGTGCAGCTG CTGGAGCACG GCCCAACCCT GGAGGAGGCC 
CCTGCCATGC TGGACAAACC AGACATCGTG TATGTGGTGG AGGGACAGCC TGCCAGCGTC 
ACCGTCACAT TCAACCATGT GGAGGCCCAG GTCGTCTGGA GGAGCTGCCG AGGGGCCCTC 
CTAGAGGCAC GGGCCGGTGT GTACGAGCTG AGCCAGCCAG ATGATGACCA GTACTGTCTT 
CGGATCTGCC GGGTGAGCCG CCGGGACATG GGGGCCCTCA CCTGCACCGC CCGAAACCGT 
CACGGCACAC AGACCTGCTC GGTCACATTG GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC 
ATCATGGAGG ACGTGGAGGT GGGGGCTGGG GAAACTGCTC GCTTTGCGGT GGTGGTCGAG 
GGAAAACCAC TGCCGGACAT CATGTGGTAC AAGGACGAGG TGCTGCTGAC CGAGAGCAGC 
CATGTGAGCT TCGTGTACGA GGAGAATGAG TGCTCCCTGG TGGTGCTCAG CACGGGGGCC 
CAGGATGGAG GCGTCTACAC CTGCACCGCC CAGAACCTGG CGGGTGAGGT CTCCTGCAAA 
GCAGAGTTGG CTGTGCATTC AGCTCAGACA GCTATGGAGG TCGAGGGGGT CGGGGAGGAT 
GAGGACCATC GAGGAAGGAG ACTCAGCGAC TTTTATGACA TCCACCAGGA GATCGGCAGG 
GGTGCTTTCT CCTACTTGCG GCGCATAGTG GAGCGTAGCT CCGGCCTGGA GTTTGCGGCC 
AAGTTCATCC CCAGCCAGGC CAAGCCAAAG GCATCAGCGC GTCGGGAGGC CCGGCTGCTG 
GCCAGGCTCC AGCACGACTG TGTCCTCTAC TTCCATGAGG CCTTCGAGAG GCGCCGGGGA 
CTGGTCATTG TCACCGAGCT CTGCACAGAG GAGCTGCTGG AGCGAATCGC CAGGAAACCC 
ACCGTGTGTG AGTCTGAGAT CCGGGCCTAT ATGCGGCAGG TGCTAGAGGG AATACACTAC 
CTGCACCAGA GCCACGTGCT GCACCTCGAT GTCAAGCCTG AGAACCTGCT GGTGTGGGAT 
GGTGCTGCGG GCGAGCAGCA GGTGCGGATC TGTGACTTTG GGAATGCCCA GGAGCTGACT 
CCAGGAGAGC CCCAGTACTG CCAGTATGGC ACACCTGAGT TTGTAGCACC CGAGATTGTC 
AATCAGAGCC CCGTGTCTGG AGTCACTGAC ATCTGGCCTG TGGGTGTTGT TGCCTTCCTC 
TGTCTGACAG GAATCTCCCC GTTTGTTGGG GAAAATGACC GGACAACATT GATGAACATC 
CGAAACTACA ACGTGGCCTT CGAGGAGACC ACATTCCTGA GCCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGTT GGTGCAGGAC CGGCTGAGAC CTACCGCAGA AGAGACCCTA 
GAACATCCTT GGTTCAAAAC TCAGGCAAAG GGCGCAGAGG TGAGCACGGA TCACCTGAAG 
CTATTCCTCT CCCGGCGGAG GTGGCAGCGC TCCCAGATCA GCTACAAATG CCACCTGGTG 
CTGCGCCCCA TCCCCGAGCT GCTGCGGGCC CCCCCAGAGC GGGTGTGGGT GACCATGCCC 
AGAAGGCCAC CCCCCAGTGG GGGGCTCTCA TCCTCCTCGG ATTCTGAAGA GGAAGAGCTG 
GAAGAGCTGC CCTCAGTGCC CCGCCCACTG CAGCCCGAGT TCTCTGGCTC CCGGGTGTCC 
CTCACAGACA TTCCCACTGA GGATGAGGCC CTGGGGACCC CAGAGACTGG GGCTGCCACC 
CCCATGGACT GGCAGGAGCA GGGAAGGGCT CCCTCTCAGG ACCAGGAGGC TCCCAGCCCA 
GAGGCCCTCC CCTCCCCAGG CCAGGAGCCC GCAGCTGGGG CTAGCCCCAG GCGGGGAGAG 
CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC CTGCCCCGGG CCGGGCCGCG GGAGCTGGGC 
CGGGGCCTGC ACAAGGCGGC GTCTGTGGAG CTGCCGCAGC GCCGGAGCCC CAGCCCGGGA 
GCCACCCGCC TGGCCCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGCCAGC GGCTGCTGCG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGT 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCT CGGGACCCCC GGATGGCACG AGCTGCCTCC 
AGCGAGGCAG CGCCCCACCA CCAGCCCCCA CTCGAGAACC GGGGCCTGCA AAAGAGCAGC 
AGCTTCTCCC AGGGTGAGGC GGAGCCCCGG GGCCGGCACC GCCGAGCGGG GGCGCCCCTC 
GAGATCCCCG TGGCCAGGCT TGGGGCCCGT AGGCTACAGG AGTCTCCTTC CCTGTCTGCC 
CTCAGCGAGG CCCAGCCATC CAGCCCTGCA CGGCCCAGCG CCCCCAAACC CAGTACCCCT 
AAGTCTGCAG AACCTTCTGC CACCACACCT AGTGATGCTC CGCAGCCCCC CGCACCCCAG 
CCTGCCCAAG ACAAGGCTCC AGAGCCCAGG CCAGAACCAG TCCGAGCCTC CAAGCCTGCA 
CCACCCCCCC AGGCCCTGCA AACCCTAGCG CTGCCCCTCA CACCCTATGC TCAGATCATT 
CAGTCCCTCC AGCTGTCAGG CCACGCCCAG GGCCCCTCGC AGGGCCCTGC CGCGCCGCCT 
TCAGAGCCCA AGCCCCACGC TGCTGTCTTT GCCAGGGTGG CCTCCCCACC TCCGGGAGCC 
CCCGAGAAGC GCGTGCCCTC AGCCGGGGGT CCCCCGGTGC TAGCCGAGAA AGCCCGAGTT 
CCCACGGTGC CCCCCAGGCC AGGCAGCAGT CTCAGTAGCA GCATCGAAAA CTTGGAGTCG 
GAGGCCGTGT TCGAGGCCAA GTTCAAGCGC AGCCGCGAGT CGCCCCTGTC GCTGGGGCTG 
CGGCTGCTGA GCCGTTCGCG CTCGGAGGAG CGCGGCCCCT TCCGTGGGGC CGAGGAGGAG 
GATGGCATAT ACCGGCCCAG CCCGGCGGGG ACCCCGCTGG AGCTGGTGCG ACGGCCTGAG 
CGCTCACGCT CGGTGCAGGA CCTCAGGGCT GTCGGAGAGC CTGGCCTCGT CCGCCGCCTC 
TCGCTGTCAC TGTCCCAGCG GCTGCGGCGG ACCCCTCCCG CGCAGCGCCA CCCGGCCTGG 
GAGGCCCGCG GCGGGGACGG AGAGAGCTCG GAGGGCGGGA GCTCGGCGCG GGGCTCCCCG 
GTGCTGGCGA TGCGCAGGCG GCTGAGCTTC ACCCTGGAGC GGCTGTCCAG CCGATTGCAG 
CGCAGTGGCA GCAGCGAGGA CTCGGGGGGC GCGTCGGGCC GCAGCACGCC GCTGTTCGGA 
CGGCTTCGCA GGGCCACGTC CGAGGGCGAG AGTCTGCGGC GCCTTGGCCT TCCGCACAAC 
CAGTTGGCCG CCCAGGCCGG CGCCACCACG CCTTCCGCCG AGTCCCTGGG CTCCGAGGCC 
AGCGCCACGT CGGGCTCCTC AGCCCCAGGG GAAAGCCGAA GCCGGCTCCG CTGGGGCTTC 
TCTCGGCCGC GGAAGGACAA GGGGTTATCG CCACCAAACC TCTCTGCCAG CGTCCAGGAG 
GAGTTGGGTC ACCAGTACGT GCGCAGTGAG TCAGACTTCC CCCCAGTCTT CCACATCAAA 
CTCAAGGACC AGGTGCTGCT GGAGGGGGAG GCAGCCACCC TGCTCTGCCT GCCAGCGGCC 
TGCCCTGCAC CGCACATCTC CTGGATGAAA GACAAGAAGT CCTTGAGGTC AGAGCCCTCA 
GTGATCATCG TGTCCTGCAA AGATGGGCGG CAGCTGCTCA GCATCCCCCG GGCGGGCAAG 
CGGCACGCCG GTCTCTATGA GTGCTCGGCC ACCAACGTAC TGGGCAGCAT CACCAGCTCC 
TGTACCGTGG CTGTGGCCCG AGTCCCAGGA AAGCTAGCTC CTCCAGAGGT ACCCCAGACC 
TACCAGGACA CGGCGCTGGT GCTGTGGAAG CCGGGAGACA GCCGGGCACC TTGCACGTAT 
ACGCTGGAGC GGCGAGTGGA TGGGGAGTCT GTGTGGCACC CTGTGAGCTC AGGCATCCCC 
GACTGTTACT ACAACGTGAC CCACCTGCCA GTTGGCGTGA CTGTGAGGTT CCGTGTGGCC 
TGTGCCAACC GTGCTGGGCA GGGGCCCTTC AGCAACTCTT CTGAGAAGGT CTTTGTCAGG 
GGTACTCAAG ATTCTTCAGC TGTGCCATCT GCTGCCCACC AAGAGGCCCC TGTCACCTCA 
AGGCCAGCCA GGGCCCGGCC TCCTGACTCT CCTACCTCAC TGGCCCCACC CCTAGCTCCT 
GCTGCCCCCA CACCCCCGTC AGTCACTGTC AGCCCCTCAT CTCCCCCCAC ACCTCCTAGC 
CAGGCCTTGT CCTCGCTCAA GGCTGTGGGT CCACCACCCC AAACCCCTCC ACGAAGACAC 
AGGGGCCTGC AGGCTGCCCG GCCAGCGGAG CCCACCCTAC CCAGTACCCA CGTCACCCCA 
AGTGAGCCCA AGCCTTTCGT CCTTGACACT GGGACCCCGA TCCCAGCCTC CACTCCTCAA 
GGGGTTAAAC CAGTGTCTTC CTCTACTCCT GTGTATGTGG TGACTTCCTT TGTGTCTGCA 
CCACCAGCCC CTGAGCCCCC AGCCCCTGAG CCCCCTCCTG AGCCTACCAA GGTGACTGTG 
CAGAGCCTCA GCCCGGCCAA GGAGGTGGTC AGCTCCCCTG GGAGCAGTCC CCGAAGCTCT 
CCCAGGCCTG AGGGTACCAC TCTTCGACAG GGTCCCCCTC AGAAACCCTA CACCTTCCTG 
GAGGAGAAAG CCAGGGGCCG CTTTGGTGTT GTGCGAGCGT GCCGGGAGAA TGCCACGGGG 
CGAACGTTCG TGGCCAAGAT CGTGCCCTAT GCTGCCGAGG GCAAGCGGCG GGTCCTGCAG 
GAGTACGAGG TGCTGCGGAC CCTGCACCAC GAGCGGATCA TGTCCCTGCA CGAGGCCTAC 
ATCACCCCTC GGTACCTCGT GCTCATTGCT GAGAGCTGTG GCAACCGGGA ACTCCTCTGT 
GGGCTCAGTG ACAGGTTCCG GTATTCTGAG GATGACGTGG CCACTTACAT GGTGCAGCTG 
CTACAAGGCC TGGACTACCT CCACGGCCAC CACGTGCTCC ACCTAGACAT CAAGCCAGAC 
AACCTGCTGC TGGCCCCTGA CAATGCCCTC AAGATTGTGG ACTTTGGCAG TGCCCAGCCC 
TACAACCCCC AGGCCCTTAG GCCCCTTGGC CACCGCACGG GCACGCTGGA GTTCATGGCT 
CCGGAGATGG TGAAGGGAGA ACCCATCGGC TCTGCCACGG ACATCTGGGG AGCGGGTGTG 
CTCACTTACA TTATGCTCAG TGGACGCTCC CCGTTCTATG AGCCAGACCC CCAGGAAACG 
GAGGCTCGGA TTGTGGGGGG CCGCTTTGAT GCCTTCCAGC TGTACCCCAA TACATCCCAG 
AGCGCCACCC TCTTCTTGCG AAAGGTTCTC TCTGTACATC CCTGGAGCCG GCCCTCCCTG 
CAGGACTGCC TGGCCCACCC ATGGTTGCAG GACGCCTACC TGATGAAGCT GCGCCGCCAG 
ACGCTCACCT TCACCACCAA CCGGCTCAAG GAGTTCCTGG GCGAGCAGCG GCGGCGCCGG 
GCTGAGGCTG CCACCCGCCA CAAGGTGCTG CTGCGCTCCT ACCCTGGCGG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_005246241.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005246298.1 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X11, mRNA.

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CloneID	OHu35758
Clone ID Related Accession (Same CDS sequence)	XM_017003158.1 , XM_005246241.1 , XM_006712193.3 , XM_017003158.2 , XM_005246240.2
Accession Version	XM_005246241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCCA GTCCCAGCCA GAACCGCCGT TCTTCTGACA CTGGCTCCAA GGCACCCCCC 
ACCTTCAAGG TCTCACTTAT GGACCAGTCA GTAAGAGAAG GCCAAGATGT CATCATGAGC 
ATCCGCGTGC AGGGGGAGCC CAAGCCTGTG GTCTCCTGGC TGAGAAACCG CCAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCGGAGGAG GCTGAGGGTG GGCTGTGCCG GCTGCGGATC 
CTGGCTGCAG AGCGTGGCGA TGCTGGTTTC TACACTTGCA AAGCGGTCAA TGAGTATGGT 
GCTCGGCAGT GCGAGGCCCG CTTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCGG GGCCCACTGA CGTGGAGGTG GATTGGCTGT GCCGTGGCCG CCTGCTGCAG 
CCTGCACTGC TCAAATGCAA GATGCATTTC GATGGCCGCA AATGCAAGCT GCTACTTACA 
TCTGTACATG AGGACGACAG TGGCGTCTAC ACCTGCAAGC TCAGCACGGC CAAAGATGAG 
CTGACCTGCA GTGCCCGGCT GACCGTGCGG CCCTCGTTGG CACCCCTGTT CACACGGCTG 
CTGGAAGATG TGGAGGTGTT GGAGGGCCGA GCTGCCCGTT TCGACTGCAA GATCAGTGGC 
ACCCCGCCCC CTGTTGTTAC CTGGACTCAT TTTGGCTGCC CCATGGAGGA GAGTGAGAAC 
TTGCGGCTGC GGCAGGACGG GGGTCTGCAC TCACTGCACA TTGCCCATGT GGGCAGCGAG 
GACGAGGGGC TCTATGCGGT CAGTGCTGTT AACACCCATG GCCAGGCCCA CTGCTCAGCC 
CAGCTGTATG TAGAAGAGCC CCGGACAGCC GCCTCAGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCCA TTCCCGAGGA GCCAGAGCAG GGTGAGCTGG AGCGGCTGTC CATTCCTGAC 
TTCCTGCGGC CACTGCAGGA CCTGGAGGTG GGACTGGCCA AGGAGGCCAT GCTAGAGTGC 
CAGGTGACCG GCCTGCCCTA CCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATCCAG 
AGCAGCGACG ACCGGCGCAT GACACAGTAC AGGGATGTCC ATCGCTTGGT GTTCCCTGCC 
GTGGGGCCTC AGCACGCCGG TGTCTACAAG AGCGTCATTG CCAACAAGCT GGGCAAAGCT 
GCCTGCTATG CCCACCTGTA TGTCACAGAT GTGGTCCCAG GCCCTCCAGA TGGCGCCCCG 
CAGGTGGTGG CTGTGACGGG GAGGATGGTC ACACTCACAT GGAACCCCCC CAGGAGTCTG 
GACATGGCCA TCGACCCGGA CTCCCTGACG TACACAGTGC AGCACCAGGT GCTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCAG GGTGGGCAGC CACAGGGCTG 
CGTAAGGGGG TCCAGCACAT CTTCCGGGTC CTCAGCACCA CTGTCAAGAG CAGCAGCAAG 
CCCTCACCCC CTTCTGAGCC TGTGCAGCTG CTGGAGCACG GCCCAACCCT GGAGGAGGCC 
CCTGCCATGC TGGACAAACC AGACATCGTG TATGTGGTGG AGGGACAGCC TGCCAGCGTC 
ACCGTCACAT TCAACCATGT GGAGGCCCAG GTCGTCTGGA GGAGCTGCCG AGGGGCCCTC 
CTAGAGGCAC GGGCCGGTGT GTACGAGCTG AGCCAGCCAG ATGATGACCA GTACTGTCTT 
CGGATCTGCC GGGTGAGCCG CCGGGACATG GGGGCCCTCA CCTGCACCGC CCGAAACCGT 
CACGGCACAC AGACCTGCTC GGTCACATTG GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC 
ATCATGGAGG ACGTGGAGGT GGGGGCTGGG GAAACTGCTC GCTTTGCGGT GGTGGTCGAG 
GGAAAACCAC TGCCGGACAT CATGTGGTAC AAGGACGAGG TGCTGCTGAC CGAGAGCAGC 
CATGTGAGCT TCGTGTACGA GGAGAATGAG TGCTCCCTGG TGGTGCTCAG CACGGGGGCC 
CAGGATGGAG GCGTCTACAC CTGCACCGCC CAGAACCTGG CGGGTGAGGT CTCCTGCAAA 
GCAGAGTTGG CTGTGCATTC AGCTCAGACA GCTATGGAGG TCGAGGGGGT CGGGGAGGAT 
GAGGACCATC GAGGAAGGAG ACTCAGCGAC TTTTATGACA TCCACCAGGA GATCGGCAGG 
GGTGCTTTCT CCTACTTGCG GCGCATAGTG GAGCGTAGCT CCGGCCTGGA GTTTGCGGCC 
AAGTTCATCC CCAGCCAGGC CAAGCCAAAG GCATCAGCGC GTCGGGAGGC CCGGCTGCTG 
GCCAGGCTCC AGCACGACTG TGTCCTCTAC TTCCATGAGG CCTTCGAGAG GCGCCGGGGA 
CTGGTCATTG TCACCGAGCT CTGCACAGAG GAGCTGCTGG AGCGAATCGC CAGGAAACCC 
ACCGTGTGTG AGTCTGAGAT CCGGGCCTAT ATGCGGCAGG TGCTAGAGGG AATACACTAC 
CTGCACCAGA GCCACGTGCT GCACCTCGAT GTCAAGCCTG AGAACCTGCT GGTGTGGGAT 
GGTGCTGCGG GCGAGCAGCA GGTGCGGATC TGTGACTTTG GGAATGCCCA GGAGCTGACT 
CCAGGAGAGC CCCAGTACTG CCAGTATGGC ACACCTGAGT TTGTAGCACC CGAGATTGTC 
AATCAGAGCC CCGTGTCTGG AGTCACTGAC ATCTGGCCTG TGGGTGTTGT TGCCTTCCTC 
TGTCTGACAG GAATCTCCCC GTTTGTTGGG GAAAATGACC GGACAACATT GATGAACATC 
CGAAACTACA ACGTGGCCTT CGAGGAGACC ACATTCCTGA GCCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGTT GGTGCAGGAC CGGCTGAGAC CTACCGCAGA AGAGACCCTA 
GAACATCCTT GGTTCAAAAC TCAGGCAAAG GGCGCAGAGG TGAGCACGGA TCACCTGAAG 
CTATTCCTCT CCCGGCGGAG GTGGCAGCGC TCCCAGATCA GCTACAAATG CCACCTGGTG 
CTGCGCCCCA TCCCCGAGCT GCTGCGGGCC CCCCCAGAGC GGGTGTGGGT GACCATGCCC 
AGAAGGCCAC CCCCCAGTGG GGGGCTCTCA TCCTCCTCGG ATTCTGAAGA GGAAGAGCTG 
GAAGAGCTGC CCTCAGTGCC CCGCCCACTG CAGCCCGAGT TCTCTGGCTC CCGGGTGTCC 
CTCACAGACA TTCCCACTGA GGATGAGGCC CTGGGGACCC CAGAGACTGG GGCTGCCACC 
CCCATGGACT GGCAGGAGCA GGGAAGGGCT CCCTCTCAGG ACCAGGAGGC TCCCAGCCCA 
GAGGCCCTCC CCTCCCCAGG CCAGGAGCCC GCAGCTGGGG CTAGCCCCAG GCGGGGAGAG 
CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC CTGCCCCGGG CCGGGCCGCG GGAGCTGGGC 
CGGGGCCTGC ACAAGGCGGC GTCTGTGGAG CTGCCGCAGC GCCGGAGCCC CAGCCCGGGA 
GCCACCCGCC TGGCCCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGCCAGC GGCTGCTGCG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGT 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCT CGGGACCCCC GGATGGCACG AGCTGCCTCC 
AGCGAGGCAG CGCCCCACCA CCAGCCCCCA CTCGAGAACC GGGGCCTGCA AAAGAGCAGC 
AGCTTCTCCC AGGGTGAGGC GGAGCCCCGG GGCCGGCACC GCCGAGCGGG GGCGCCCCTC 
GAGATCCCCG TGGCCAGGCT TGGGGCCCGT AGGCTACAGG AGTCTCCTTC CCTGTCTGCC 
CTCAGCGAGG CCCAGCCATC CAGCCCTGCA CGGCCCAGCG CCCCCAAACC CAGTACCCCT 
AAGTCTGCAG AACCTTCTGC CACCACACCT AGTGATGCTC CGCAGCCCCC CGCACCCCAG 
CCTGCCCAAG ACAAGGCTCC AGAGCCCAGG CCAGAACCAG TCCGAGCCTC CAAGCCTGCA 
CCACCCCCCC AGGCCCTGCA AACCCTAGCG CTGCCCCTCA CACCCTATGC TCAGATCATT 
CAGTCCCTCC AGCTGTCAGG CCACGCCCAG GGCCCCTCGC AGGGCCCTGC CGCGCCGCCT 
TCAGAGCCCA AGCCCCACGC TGCTGTCTTT GCCAGGGTGG CCTCCCCACC TCCGGGAGCC 
CCCGAGAAGC GCGTGCCCTC AGCCGGGGGT CCCCCGGTGC TAGCCGAGAA AGCCCGAGTT 
CCCACGGTGC CCCCCAGGCC AGGCAGCAGT CTCAGTAGCA GCATCGAAAA CTTGGAGTCG 
GAGGCCGTGT TCGAGGCCAA GTTCAAGCGC AGCCGCGAGT CGCCCCTGTC GCTGGGGCTG 
CGGCTGCTGA GCCGTTCGCG CTCGGAGGAG CGCGGCCCCT TCCGTGGGGC CGAGGAGGAG 
GATGGCATAT ACCGGCCCAG CCCGGCGGGG ACCCCGCTGG AGCTGGTGCG ACGGCCTGAG 
CGCTCACGCT CGGTGCAGGA CCTCAGGGCT GTCGGAGAGC CTGGCCTCGT CCGCCGCCTC 
TCGCTGTCAC TGTCCCAGCG GCTGCGGCGG ACCCCTCCCG CGCAGCGCCA CCCGGCCTGG 
GAGGCCCGCG GCGGGGACGG AGAGAGCTCG GAGGGCGGGA GCTCGGCGCG GGGCTCCCCG 
GTGCTGGCGA TGCGCAGGCG GCTGAGCTTC ACCCTGGAGC GGCTGTCCAG CCGATTGCAG 
CGCAGTGGCA GCAGCGAGGA CTCGGGGGGC GCGTCGGGCC GCAGCACGCC GCTGTTCGGA 
CGGCTTCGCA GGGCCACGTC CGAGGGCGAG AGTCTGCGGC GCCTTGGCCT TCCGCACAAC 
CAGTTGGCCG CCCAGGCCGG CGCCACCACG CCTTCCGCCG AGTCCCTGGG CTCCGAGGCC 
AGCGCCACGT CGGGCTCCTC AGCCCCAGGG GAAAGCCGAA GCCGGCTCCG CTGGGGCTTC 
TCTCGGCCGC GGAAGGACAA GGGGTTATCG CCACCAAACC TCTCTGCCAG CGTCCAGGAG 
GAGTTGGGTC ACCAGTACGT GCGCAGTGAG TCAGACTTCC CCCCAGTCTT CCACATCAAA 
CTCAAGGACC AGGTGCTGCT GGAGGGGGAG GCAGCCACCC TGCTCTGCCT GCCAGCGGCC 
TGCCCTGCAC CGCACATCTC CTGGATGAAA GACAAGAAGT CCTTGAGGTC AGAGCCCTCA 
GTGATCATCG TGTCCTGCAA AGATGGGCGG CAGCTGCTCA GCATCCCCCG GGCGGGCAAG 
CGGCACGCCG GTCTCTATGA GTGCTCGGCC ACCAACGTAC TGGGCAGCAT CACCAGCTCC 
TGTACCGTGG CTGTGGCCCG AGTCCCAGGA AAGCTAGCTC CTCCAGAGGT ACCCCAGACC 
TACCAGGACA CGGCGCTGGT GCTGTGGAAG CCGGGAGACA GCCGGGCACC TTGCACGTAT 
ACGCTGGAGC GGCGAGTGGA TGGGGAGTCT GTGTGGCACC CTGTGAGCTC AGGCATCCCC 
GACTGTTACT ACAACGTGAC CCACCTGCCA GTTGGCGTGA CTGTGAGGTT CCGTGTGGCC 
TGTGCCAACC GTGCTGGGCA GGGGCCCTTC AGCAACTCTT CTGAGAAGGT CTTTGTCAGG 
GGTACTCAAG ATTCTTCAGC TGTGCCATCT GCTGCCCACC AAGAGGCCCC TGTCACCTCA 
AGGCCAGCCA GGGCCCGGCC TCCTGACTCT CCTACCTCAC TGGCCCCACC CCTAGCTCCT 
GCTGCCCCCA CACCCCCGTC AGTCACTGTC AGCCCCTCAT CTCCCCCCAC ACCTCCTAGC 
CAGGCCTTGT CCTCGCTCAA GGCTGTGGGT CCACCACCCC AAACCCCTCC ACGAAGACAC 
AGGGGCCTGC AGGCTGCCCG GCCAGCGGAG CCCACCCTAC CCAGTACCCA CGTCACCCCA 
AGTGAGCCCA AGCCTTTCGT CCTTGACACT GGGACCCCGA TCCCAGCCTC CACTCCTCAA 
GGGGTTAAAC CAGTGTCTTC CTCTACTCCT GTGTATGTGG TGACTTCCTT TGTGTCTGCA 
CCACCAGCCC CTGAGCCCCC AGCCCCTGAG CCCCCTCCTG AGCCTACCAA GGTGACTGTG 
CAGAGCCTCA GCCCGGCCAA GGAGGTGGTC AGCTCCCCTG GGAGCAGTCC CCGAAGCTCT 
CCCAGGCCTG AGGGTACCAC TCTTCGACAG GGTCCCCCTC AGAAACCCTA CACCTTCCTG 
GAGGAGAAAG CCAGGGGCCG CTTTGGTGTT GTGCGAGCGT GCCGGGAGAA TGCCACGGGG 
CGAACGTTCG TGGCCAAGAT CGTGCCCTAT GCTGCCGAGG GCAAGCGGCG GGTCCTGCAG 
GAGTACGAGG TGCTGCGGAC CCTGCACCAC GAGCGGATCA TGTCCCTGCA CGAGGCCTAC 
ATCACCCCTC GGTACCTCGT GCTCATTGCT GAGAGCTGTG GCAACCGGGA ACTCCTCTGT 
GGGCTCAGTG ACAGGTTCCG GTATTCTGAG GATGACGTGG CCACTTACAT GGTGCAGCTG 
CTACAAGGCC TGGACTACCT CCACGGCCAC CACGTGCTCC ACCTAGACAT CAAGCCAGAC 
AACCTGCTGC TGGCCCCTGA CAATGCCCTC AAGATTGTGG ACTTTGGCAG TGCCCAGCCC 
TACAACCCCC AGGCCCTTAG GCCCCTTGGC CACCGCACGG GCACGCTGGA GTTCATGGCT 
CCGGAGATGG TGAAGGGAGA ACCCATCGGC TCTGCCACGG ACATCTGGGG AGCGGGTGTG 
CTCACTTACA TTATGCTCAG TGGACGCTCC CCGTTCTATG AGCCAGACCC CCAGGAAACG 
GAGGCTCGGA TTGTGGGGGG CCGCTTTGAT GCCTTCCAGC TGTACCCCAA TACATCCCAG 
AGCGCCACCC TCTTCTTGCG AAAGGTTCTC TCTGTACATC CCTGGAGCCG GCCCTCCCTG 
CAGGACTGCC TGGCCCACCC ATGGTTGCAG GACGCCTACC TGATGAAGCT GCGCCGCCAG 
ACGCTCACCT TCACCACCAA CCGGCTCAAG GAGTTCCTGG GCGAGCAGCG GCGGCGCCGG 
GCTGAGGCTG CCACCCGCCA CAAGGTGCTG CTGCGCTCCT ACCCTGGCGG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005246298.1
CDS	187..7443
Translation	MKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPTDVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARFDCKISGTPPPVVTWTHFGCPMEESENLRLRQDGGLHSLHIAHVGSEDEGLYAVSAVNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEQGELERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDVHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPQVVAVTGRMVTLTWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPGWAATGLRKGVQHIFRVLSTTVKSSSKPSPPSEPVQLLEHGPTLEEAPAMLDKPDIVYVVEGQPASVTVTFNHVEAQVVWRSCRGALLEARAGVYELSQPDDDQYCLRICRVSRRDMGALTCTARNRHGTQTCSVTLELAEAPRFESIMEDVEVGAGETARFAVVVEGKPLPDIMWYKDEVLLTESSHVSFVYEENECSLVVLSTGAQDGGVYTCTAQNLAGEVSCKAELAVHSAQTAMEVEGVGEDEDHRGRRLSDFYDIHQEIGRGAFSYLRRIVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHDCVLYFHEAFERRRGLVIVTELCTEELLERIARKPTVCESEIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTQAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGELRRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQPPAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPSEPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEAKFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAVGEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAESLGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPHISWMKDKKSLRSEPSVIIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARVPGKLAPPEVPQTYQDTALVLWKPGDSRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTHLPVGVTVRFRVACANRAGQGPFSNSSEKVFVRGTQDSSAVPSAAHQEAPVTSRPARARPPDSPTSLAPPLAPAAPTPPSVTVSPSSPPTPPSQALSSLKAVGPPPQTPPRRHRGLQAARPAEPTLPSTHVTPSEPKPFVLDTGTPIPASTPQGVKPVSSSTPVYVVTSFVSAPPAPEPPAPEPPPEPTKVTVQSLSPAKEVVSSPGSSPRSSPRPEGTTLRQGPPQKPYTFLEEKARGRFGVVRACRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERIMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYMVQLLQGLDYLHGHHVLHLDIKPDNLLLAPDNALKIVDFGSAQPYNPQALRPLGHRTGTLEFMAPEMVKGEPIGSATDIWGAGVLTYIMLSGRSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGGP

Target ORF information:

RefSeq Version	XM_005246241.1
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246241.1

ORF Insert Sequence:

ATGAAGCCCA GTCCCAGCCA GAACCGCCGT TCTTCTGACA CTGGCTCCAA GGCACCCCCC 
ACCTTCAAGG TCTCACTTAT GGACCAGTCA GTAAGAGAAG GCCAAGATGT CATCATGAGC 
ATCCGCGTGC AGGGGGAGCC CAAGCCTGTG GTCTCCTGGC TGAGAAACCG CCAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCGGAGGAG GCTGAGGGTG GGCTGTGCCG GCTGCGGATC 
CTGGCTGCAG AGCGTGGCGA TGCTGGTTTC TACACTTGCA AAGCGGTCAA TGAGTATGGT 
GCTCGGCAGT GCGAGGCCCG CTTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCGG GGCCCACTGA CGTGGAGGTG GATTGGCTGT GCCGTGGCCG CCTGCTGCAG 
CCTGCACTGC TCAAATGCAA GATGCATTTC GATGGCCGCA AATGCAAGCT GCTACTTACA 
TCTGTACATG AGGACGACAG TGGCGTCTAC ACCTGCAAGC TCAGCACGGC CAAAGATGAG 
CTGACCTGCA GTGCCCGGCT GACCGTGCGG CCCTCGTTGG CACCCCTGTT CACACGGCTG 
CTGGAAGATG TGGAGGTGTT GGAGGGCCGA GCTGCCCGTT TCGACTGCAA GATCAGTGGC 
ACCCCGCCCC CTGTTGTTAC CTGGACTCAT TTTGGCTGCC CCATGGAGGA GAGTGAGAAC 
TTGCGGCTGC GGCAGGACGG GGGTCTGCAC TCACTGCACA TTGCCCATGT GGGCAGCGAG 
GACGAGGGGC TCTATGCGGT CAGTGCTGTT AACACCCATG GCCAGGCCCA CTGCTCAGCC 
CAGCTGTATG TAGAAGAGCC CCGGACAGCC GCCTCAGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCCA TTCCCGAGGA GCCAGAGCAG GGTGAGCTGG AGCGGCTGTC CATTCCTGAC 
TTCCTGCGGC CACTGCAGGA CCTGGAGGTG GGACTGGCCA AGGAGGCCAT GCTAGAGTGC 
CAGGTGACCG GCCTGCCCTA CCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATCCAG 
AGCAGCGACG ACCGGCGCAT GACACAGTAC AGGGATGTCC ATCGCTTGGT GTTCCCTGCC 
GTGGGGCCTC AGCACGCCGG TGTCTACAAG AGCGTCATTG CCAACAAGCT GGGCAAAGCT 
GCCTGCTATG CCCACCTGTA TGTCACAGAT GTGGTCCCAG GCCCTCCAGA TGGCGCCCCG 
CAGGTGGTGG CTGTGACGGG GAGGATGGTC ACACTCACAT GGAACCCCCC CAGGAGTCTG 
GACATGGCCA TCGACCCGGA CTCCCTGACG TACACAGTGC AGCACCAGGT GCTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCAG GGTGGGCAGC CACAGGGCTG 
CGTAAGGGGG TCCAGCACAT CTTCCGGGTC CTCAGCACCA CTGTCAAGAG CAGCAGCAAG 
CCCTCACCCC CTTCTGAGCC TGTGCAGCTG CTGGAGCACG GCCCAACCCT GGAGGAGGCC 
CCTGCCATGC TGGACAAACC AGACATCGTG TATGTGGTGG AGGGACAGCC TGCCAGCGTC 
ACCGTCACAT TCAACCATGT GGAGGCCCAG GTCGTCTGGA GGAGCTGCCG AGGGGCCCTC 
CTAGAGGCAC GGGCCGGTGT GTACGAGCTG AGCCAGCCAG ATGATGACCA GTACTGTCTT 
CGGATCTGCC GGGTGAGCCG CCGGGACATG GGGGCCCTCA CCTGCACCGC CCGAAACCGT 
CACGGCACAC AGACCTGCTC GGTCACATTG GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC 
ATCATGGAGG ACGTGGAGGT GGGGGCTGGG GAAACTGCTC GCTTTGCGGT GGTGGTCGAG 
GGAAAACCAC TGCCGGACAT CATGTGGTAC AAGGACGAGG TGCTGCTGAC CGAGAGCAGC 
CATGTGAGCT TCGTGTACGA GGAGAATGAG TGCTCCCTGG TGGTGCTCAG CACGGGGGCC 
CAGGATGGAG GCGTCTACAC CTGCACCGCC CAGAACCTGG CGGGTGAGGT CTCCTGCAAA 
GCAGAGTTGG CTGTGCATTC AGCTCAGACA GCTATGGAGG TCGAGGGGGT CGGGGAGGAT 
GAGGACCATC GAGGAAGGAG ACTCAGCGAC TTTTATGACA TCCACCAGGA GATCGGCAGG 
GGTGCTTTCT CCTACTTGCG GCGCATAGTG GAGCGTAGCT CCGGCCTGGA GTTTGCGGCC 
AAGTTCATCC CCAGCCAGGC CAAGCCAAAG GCATCAGCGC GTCGGGAGGC CCGGCTGCTG 
GCCAGGCTCC AGCACGACTG TGTCCTCTAC TTCCATGAGG CCTTCGAGAG GCGCCGGGGA 
CTGGTCATTG TCACCGAGCT CTGCACAGAG GAGCTGCTGG AGCGAATCGC CAGGAAACCC 
ACCGTGTGTG AGTCTGAGAT CCGGGCCTAT ATGCGGCAGG TGCTAGAGGG AATACACTAC 
CTGCACCAGA GCCACGTGCT GCACCTCGAT GTCAAGCCTG AGAACCTGCT GGTGTGGGAT 
GGTGCTGCGG GCGAGCAGCA GGTGCGGATC TGTGACTTTG GGAATGCCCA GGAGCTGACT 
CCAGGAGAGC CCCAGTACTG CCAGTATGGC ACACCTGAGT TTGTAGCACC CGAGATTGTC 
AATCAGAGCC CCGTGTCTGG AGTCACTGAC ATCTGGCCTG TGGGTGTTGT TGCCTTCCTC 
TGTCTGACAG GAATCTCCCC GTTTGTTGGG GAAAATGACC GGACAACATT GATGAACATC 
CGAAACTACA ACGTGGCCTT CGAGGAGACC ACATTCCTGA GCCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGTT GGTGCAGGAC CGGCTGAGAC CTACCGCAGA AGAGACCCTA 
GAACATCCTT GGTTCAAAAC TCAGGCAAAG GGCGCAGAGG TGAGCACGGA TCACCTGAAG 
CTATTCCTCT CCCGGCGGAG GTGGCAGCGC TCCCAGATCA GCTACAAATG CCACCTGGTG 
CTGCGCCCCA TCCCCGAGCT GCTGCGGGCC CCCCCAGAGC GGGTGTGGGT GACCATGCCC 
AGAAGGCCAC CCCCCAGTGG GGGGCTCTCA TCCTCCTCGG ATTCTGAAGA GGAAGAGCTG 
GAAGAGCTGC CCTCAGTGCC CCGCCCACTG CAGCCCGAGT TCTCTGGCTC CCGGGTGTCC 
CTCACAGACA TTCCCACTGA GGATGAGGCC CTGGGGACCC CAGAGACTGG GGCTGCCACC 
CCCATGGACT GGCAGGAGCA GGGAAGGGCT CCCTCTCAGG ACCAGGAGGC TCCCAGCCCA 
GAGGCCCTCC CCTCCCCAGG CCAGGAGCCC GCAGCTGGGG CTAGCCCCAG GCGGGGAGAG 
CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC CTGCCCCGGG CCGGGCCGCG GGAGCTGGGC 
CGGGGCCTGC ACAAGGCGGC GTCTGTGGAG CTGCCGCAGC GCCGGAGCCC CAGCCCGGGA 
GCCACCCGCC TGGCCCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGCCAGC GGCTGCTGCG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGT 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCT CGGGACCCCC GGATGGCACG AGCTGCCTCC 
AGCGAGGCAG CGCCCCACCA CCAGCCCCCA CTCGAGAACC GGGGCCTGCA AAAGAGCAGC 
AGCTTCTCCC AGGGTGAGGC GGAGCCCCGG GGCCGGCACC GCCGAGCGGG GGCGCCCCTC 
GAGATCCCCG TGGCCAGGCT TGGGGCCCGT AGGCTACAGG AGTCTCCTTC CCTGTCTGCC 
CTCAGCGAGG CCCAGCCATC CAGCCCTGCA CGGCCCAGCG CCCCCAAACC CAGTACCCCT 
AAGTCTGCAG AACCTTCTGC CACCACACCT AGTGATGCTC CGCAGCCCCC CGCACCCCAG 
CCTGCCCAAG ACAAGGCTCC AGAGCCCAGG CCAGAACCAG TCCGAGCCTC CAAGCCTGCA 
CCACCCCCCC AGGCCCTGCA AACCCTAGCG CTGCCCCTCA CACCCTATGC TCAGATCATT 
CAGTCCCTCC AGCTGTCAGG CCACGCCCAG GGCCCCTCGC AGGGCCCTGC CGCGCCGCCT 
TCAGAGCCCA AGCCCCACGC TGCTGTCTTT GCCAGGGTGG CCTCCCCACC TCCGGGAGCC 
CCCGAGAAGC GCGTGCCCTC AGCCGGGGGT CCCCCGGTGC TAGCCGAGAA AGCCCGAGTT 
CCCACGGTGC CCCCCAGGCC AGGCAGCAGT CTCAGTAGCA GCATCGAAAA CTTGGAGTCG 
GAGGCCGTGT TCGAGGCCAA GTTCAAGCGC AGCCGCGAGT CGCCCCTGTC GCTGGGGCTG 
CGGCTGCTGA GCCGTTCGCG CTCGGAGGAG CGCGGCCCCT TCCGTGGGGC CGAGGAGGAG 
GATGGCATAT ACCGGCCCAG CCCGGCGGGG ACCCCGCTGG AGCTGGTGCG ACGGCCTGAG 
CGCTCACGCT CGGTGCAGGA CCTCAGGGCT GTCGGAGAGC CTGGCCTCGT CCGCCGCCTC 
TCGCTGTCAC TGTCCCAGCG GCTGCGGCGG ACCCCTCCCG CGCAGCGCCA CCCGGCCTGG 
GAGGCCCGCG GCGGGGACGG AGAGAGCTCG GAGGGCGGGA GCTCGGCGCG GGGCTCCCCG 
GTGCTGGCGA TGCGCAGGCG GCTGAGCTTC ACCCTGGAGC GGCTGTCCAG CCGATTGCAG 
CGCAGTGGCA GCAGCGAGGA CTCGGGGGGC GCGTCGGGCC GCAGCACGCC GCTGTTCGGA 
CGGCTTCGCA GGGCCACGTC CGAGGGCGAG AGTCTGCGGC GCCTTGGCCT TCCGCACAAC 
CAGTTGGCCG CCCAGGCCGG CGCCACCACG CCTTCCGCCG AGTCCCTGGG CTCCGAGGCC 
AGCGCCACGT CGGGCTCCTC AGCCCCAGGG GAAAGCCGAA GCCGGCTCCG CTGGGGCTTC 
TCTCGGCCGC GGAAGGACAA GGGGTTATCG CCACCAAACC TCTCTGCCAG CGTCCAGGAG 
GAGTTGGGTC ACCAGTACGT GCGCAGTGAG TCAGACTTCC CCCCAGTCTT CCACATCAAA 
CTCAAGGACC AGGTGCTGCT GGAGGGGGAG GCAGCCACCC TGCTCTGCCT GCCAGCGGCC 
TGCCCTGCAC CGCACATCTC CTGGATGAAA GACAAGAAGT CCTTGAGGTC AGAGCCCTCA 
GTGATCATCG TGTCCTGCAA AGATGGGCGG CAGCTGCTCA GCATCCCCCG GGCGGGCAAG 
CGGCACGCCG GTCTCTATGA GTGCTCGGCC ACCAACGTAC TGGGCAGCAT CACCAGCTCC 
TGTACCGTGG CTGTGGCCCG AGTCCCAGGA AAGCTAGCTC CTCCAGAGGT ACCCCAGACC 
TACCAGGACA CGGCGCTGGT GCTGTGGAAG CCGGGAGACA GCCGGGCACC TTGCACGTAT 
ACGCTGGAGC GGCGAGTGGA TGGGGAGTCT GTGTGGCACC CTGTGAGCTC AGGCATCCCC 
GACTGTTACT ACAACGTGAC CCACCTGCCA GTTGGCGTGA CTGTGAGGTT CCGTGTGGCC 
TGTGCCAACC GTGCTGGGCA GGGGCCCTTC AGCAACTCTT CTGAGAAGGT CTTTGTCAGG 
GGTACTCAAG ATTCTTCAGC TGTGCCATCT GCTGCCCACC AAGAGGCCCC TGTCACCTCA 
AGGCCAGCCA GGGCCCGGCC TCCTGACTCT CCTACCTCAC TGGCCCCACC CCTAGCTCCT 
GCTGCCCCCA CACCCCCGTC AGTCACTGTC AGCCCCTCAT CTCCCCCCAC ACCTCCTAGC 
CAGGCCTTGT CCTCGCTCAA GGCTGTGGGT CCACCACCCC AAACCCCTCC ACGAAGACAC 
AGGGGCCTGC AGGCTGCCCG GCCAGCGGAG CCCACCCTAC CCAGTACCCA CGTCACCCCA 
AGTGAGCCCA AGCCTTTCGT CCTTGACACT GGGACCCCGA TCCCAGCCTC CACTCCTCAA 
GGGGTTAAAC CAGTGTCTTC CTCTACTCCT GTGTATGTGG TGACTTCCTT TGTGTCTGCA 
CCACCAGCCC CTGAGCCCCC AGCCCCTGAG CCCCCTCCTG AGCCTACCAA GGTGACTGTG 
CAGAGCCTCA GCCCGGCCAA GGAGGTGGTC AGCTCCCCTG GGAGCAGTCC CCGAAGCTCT 
CCCAGGCCTG AGGGTACCAC TCTTCGACAG GGTCCCCCTC AGAAACCCTA CACCTTCCTG 
GAGGAGAAAG CCAGGGGCCG CTTTGGTGTT GTGCGAGCGT GCCGGGAGAA TGCCACGGGG 
CGAACGTTCG TGGCCAAGAT CGTGCCCTAT GCTGCCGAGG GCAAGCGGCG GGTCCTGCAG 
GAGTACGAGG TGCTGCGGAC CCTGCACCAC GAGCGGATCA TGTCCCTGCA CGAGGCCTAC 
ATCACCCCTC GGTACCTCGT GCTCATTGCT GAGAGCTGTG GCAACCGGGA ACTCCTCTGT 
GGGCTCAGTG ACAGGTTCCG GTATTCTGAG GATGACGTGG CCACTTACAT GGTGCAGCTG 
CTACAAGGCC TGGACTACCT CCACGGCCAC CACGTGCTCC ACCTAGACAT CAAGCCAGAC 
AACCTGCTGC TGGCCCCTGA CAATGCCCTC AAGATTGTGG ACTTTGGCAG TGCCCAGCCC 
TACAACCCCC AGGCCCTTAG GCCCCTTGGC CACCGCACGG GCACGCTGGA GTTCATGGCT 
CCGGAGATGG TGAAGGGAGA ACCCATCGGC TCTGCCACGG ACATCTGGGG AGCGGGTGTG 
CTCACTTACA TTATGCTCAG TGGACGCTCC CCGTTCTATG AGCCAGACCC CCAGGAAACG 
GAGGCTCGGA TTGTGGGGGG CCGCTTTGAT GCCTTCCAGC TGTACCCCAA TACATCCCAG 
AGCGCCACCC TCTTCTTGCG AAAGGTTCTC TCTGTACATC CCTGGAGCCG GCCCTCCCTG 
CAGGACTGCC TGGCCCACCC ATGGTTGCAG GACGCCTACC TGATGAAGCT GCGCCGCCAG 
ACGCTCACCT TCACCACCAA CCGGCTCAAG GAGTTCCTGG GCGAGCAGCG GCGGCGCCGG 
GCTGAGGCTG CCACCCGCCA CAAGGTGCTG CTGCGCTCCT ACCCTGGCGG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_006712193.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712256.1 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X12, mRNA.

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CloneID	OHu35758
Clone ID Related Accession (Same CDS sequence)	XM_017003158.1 , XM_005246241.1 , XM_006712193.3 , XM_017003158.2 , XM_005246240.2
Accession Version	XM_006712193.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712193.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCCA GTCCCAGCCA GAACCGCCGT TCTTCTGACA CTGGCTCCAA GGCACCCCCC 
ACCTTCAAGG TCTCACTTAT GGACCAGTCA GTAAGAGAAG GCCAAGATGT CATCATGAGC 
ATCCGCGTGC AGGGGGAGCC CAAGCCTGTG GTCTCCTGGC TGAGAAACCG CCAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCGGAGGAG GCTGAGGGTG GGCTGTGCCG GCTGCGGATC 
CTGGCTGCAG AGCGTGGCGA TGCTGGTTTC TACACTTGCA AAGCGGTCAA TGAGTATGGT 
GCTCGGCAGT GCGAGGCCCG CTTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCGG GGCCCACTGA CGTGGAGGTG GATTGGCTGT GCCGTGGCCG CCTGCTGCAG 
CCTGCACTGC TCAAATGCAA GATGCATTTC GATGGCCGCA AATGCAAGCT GCTACTTACA 
TCTGTACATG AGGACGACAG TGGCGTCTAC ACCTGCAAGC TCAGCACGGC CAAAGATGAG 
CTGACCTGCA GTGCCCGGCT GACCGTGCGG CCCTCGTTGG CACCCCTGTT CACACGGCTG 
CTGGAAGATG TGGAGGTGTT GGAGGGCCGA GCTGCCCGTT TCGACTGCAA GATCAGTGGC 
ACCCCGCCCC CTGTTGTTAC CTGGACTCAT TTTGGCTGCC CCATGGAGGA GAGTGAGAAC 
TTGCGGCTGC GGCAGGACGG GGGTCTGCAC TCACTGCACA TTGCCCATGT GGGCAGCGAG 
GACGAGGGGC TCTATGCGGT CAGTGCTGTT AACACCCATG GCCAGGCCCA CTGCTCAGCC 
CAGCTGTATG TAGAAGAGCC CCGGACAGCC GCCTCAGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCCA TTCCCGAGGA GCCAGAGCAG GGTGAGCTGG AGCGGCTGTC CATTCCTGAC 
TTCCTGCGGC CACTGCAGGA CCTGGAGGTG GGACTGGCCA AGGAGGCCAT GCTAGAGTGC 
CAGGTGACCG GCCTGCCCTA CCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATCCAG 
AGCAGCGACG ACCGGCGCAT GACACAGTAC AGGGATGTCC ATCGCTTGGT GTTCCCTGCC 
GTGGGGCCTC AGCACGCCGG TGTCTACAAG AGCGTCATTG CCAACAAGCT GGGCAAAGCT 
GCCTGCTATG CCCACCTGTA TGTCACAGAT GTGGTCCCAG GCCCTCCAGA TGGCGCCCCG 
CAGGTGGTGG CTGTGACGGG GAGGATGGTC ACACTCACAT GGAACCCCCC CAGGAGTCTG 
GACATGGCCA TCGACCCGGA CTCCCTGACG TACACAGTGC AGCACCAGGT GCTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCAG GGTGGGCAGC CACAGGGCTG 
CGTAAGGGGG TCCAGCACAT CTTCCGGGTC CTCAGCACCA CTGTCAAGAG CAGCAGCAAG 
CCCTCACCCC CTTCTGAGCC TGTGCAGCTG CTGGAGCACG GCCCAACCCT GGAGGAGGCC 
CCTGCCATGC TGGACAAACC AGACATCGTG TATGTGGTGG AGGGACAGCC TGCCAGCGTC 
ACCGTCACAT TCAACCATGT GGAGGCCCAG GTCGTCTGGA GGAGCTGCCG AGGGGCCCTC 
CTAGAGGCAC GGGCCGGTGT GTACGAGCTG AGCCAGCCAG ATGATGACCA GTACTGTCTT 
CGGATCTGCC GGGTGAGCCG CCGGGACATG GGGGCCCTCA CCTGCACCGC CCGAAACCGT 
CACGGCACAC AGACCTGCTC GGTCACATTG GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC 
ATCATGGAGG ACGTGGAGGT GGGGGCTGGG GAAACTGCTC GCTTTGCGGT GGTGGTCGAG 
GGAAAACCAC TGCCGGACAT CATGTGGTAC AAGGACGAGG TGCTGCTGAC CGAGAGCAGC 
CATGTGAGCT TCGTGTACGA GGAGAATGAG TGCTCCCTGG TGGTGCTCAG CACGGGGGCC 
CAGGATGGAG GCGTCTACAC CTGCACCGCC CAGAACCTGG CGGGTGAGGT CTCCTGCAAA 
GCAGAGTTGG CTGTGCATTC AGCTCAGACA GCTATGGAGG TCGAGGGGGT CGGGGAGGAT 
GAGGACCATC GAGGAAGGAG ACTCAGCGAC TTTTATGACA TCCACCAGGA GATCGGCAGG 
GGTGCTTTCT CCTACTTGCG GCGCATAGTG GAGCGTAGCT CCGGCCTGGA GTTTGCGGCC 
AAGTTCATCC CCAGCCAGGC CAAGCCAAAG GCATCAGCGC GTCGGGAGGC CCGGCTGCTG 
GCCAGGCTCC AGCACGACTG TGTCCTCTAC TTCCATGAGG CCTTCGAGAG GCGCCGGGGA 
CTGGTCATTG TCACCGAGCT CTGCACAGAG GAGCTGCTGG AGCGAATCGC CAGGAAACCC 
ACCGTGTGTG AGTCTGAGAT CCGGGCCTAT ATGCGGCAGG TGCTAGAGGG AATACACTAC 
CTGCACCAGA GCCACGTGCT GCACCTCGAT GTCAAGCCTG AGAACCTGCT GGTGTGGGAT 
GGTGCTGCGG GCGAGCAGCA GGTGCGGATC TGTGACTTTG GGAATGCCCA GGAGCTGACT 
CCAGGAGAGC CCCAGTACTG CCAGTATGGC ACACCTGAGT TTGTAGCACC CGAGATTGTC 
AATCAGAGCC CCGTGTCTGG AGTCACTGAC ATCTGGCCTG TGGGTGTTGT TGCCTTCCTC 
TGTCTGACAG GAATCTCCCC GTTTGTTGGG GAAAATGACC GGACAACATT GATGAACATC 
CGAAACTACA ACGTGGCCTT CGAGGAGACC ACATTCCTGA GCCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGTT GGTGCAGGAC CGGCTGAGAC CTACCGCAGA AGAGACCCTA 
GAACATCCTT GGTTCAAAAC TCAGGCAAAG GGCGCAGAGG TGAGCACGGA TCACCTGAAG 
CTATTCCTCT CCCGGCGGAG GTGGCAGCGC TCCCAGATCA GCTACAAATG CCACCTGGTG 
CTGCGCCCCA TCCCCGAGCT GCTGCGGGCC CCCCCAGAGC GGGTGTGGGT GACCATGCCC 
AGAAGGCCAC CCCCCAGTGG GGGGCTCTCA TCCTCCTCGG ATTCTGAAGA GGAAGAGCTG 
GAAGAGCTGC CCTCAGTGCC CCGCCCACTG CAGCCCGAGT TCTCTGGCTC CCGGGTGTCC 
CTCACAGACA TTCCCACTGA GGATGAGGCC CTGGGGACCC CAGAGACTGG GGCTGCCACC 
CCCATGGACT GGCAGGAGCA GGGAAGGGCT CCCTCTCAGG ACCAGGAGGC TCCCAGCCCA 
GAGGCCCTCC CCTCCCCAGG CCAGGAGCCC GCAGCTGGGG CTAGCCCCAG GCGGGGAGAG 
CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC CTGCCCCGGG CCGGGCCGCG GGAGCTGGGC 
CGGGGCCTGC ACAAGGCGGC GTCTGTGGAG CTGCCGCAGC GCCGGAGCCC CAGCCCGGGA 
GCCACCCGCC TGGCCCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGCCAGC GGCTGCTGCG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGT 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCT CGGGACCCCC GGATGGCACG AGCTGCCTCC 
AGCGAGGCAG CGCCCCACCA CCAGCCCCCA CTCGAGAACC GGGGCCTGCA AAAGAGCAGC 
AGCTTCTCCC AGGGTGAGGC GGAGCCCCGG GGCCGGCACC GCCGAGCGGG GGCGCCCCTC 
GAGATCCCCG TGGCCAGGCT TGGGGCCCGT AGGCTACAGG AGTCTCCTTC CCTGTCTGCC 
CTCAGCGAGG CCCAGCCATC CAGCCCTGCA CGGCCCAGCG CCCCCAAACC CAGTACCCCT 
AAGTCTGCAG AACCTTCTGC CACCACACCT AGTGATGCTC CGCAGCCCCC CGCACCCCAG 
CCTGCCCAAG ACAAGGCTCC AGAGCCCAGG CCAGAACCAG TCCGAGCCTC CAAGCCTGCA 
CCACCCCCCC AGGCCCTGCA AACCCTAGCG CTGCCCCTCA CACCCTATGC TCAGATCATT 
CAGTCCCTCC AGCTGTCAGG CCACGCCCAG GGCCCCTCGC AGGGCCCTGC CGCGCCGCCT 
TCAGAGCCCA AGCCCCACGC TGCTGTCTTT GCCAGGGTGG CCTCCCCACC TCCGGGAGCC 
CCCGAGAAGC GCGTGCCCTC AGCCGGGGGT CCCCCGGTGC TAGCCGAGAA AGCCCGAGTT 
CCCACGGTGC CCCCCAGGCC AGGCAGCAGT CTCAGTAGCA GCATCGAAAA CTTGGAGTCG 
GAGGCCGTGT TCGAGGCCAA GTTCAAGCGC AGCCGCGAGT CGCCCCTGTC GCTGGGGCTG 
CGGCTGCTGA GCCGTTCGCG CTCGGAGGAG CGCGGCCCCT TCCGTGGGGC CGAGGAGGAG 
GATGGCATAT ACCGGCCCAG CCCGGCGGGG ACCCCGCTGG AGCTGGTGCG ACGGCCTGAG 
CGCTCACGCT CGGTGCAGGA CCTCAGGGCT GTCGGAGAGC CTGGCCTCGT CCGCCGCCTC 
TCGCTGTCAC TGTCCCAGCG GCTGCGGCGG ACCCCTCCCG CGCAGCGCCA CCCGGCCTGG 
GAGGCCCGCG GCGGGGACGG AGAGAGCTCG GAGGGCGGGA GCTCGGCGCG GGGCTCCCCG 
GTGCTGGCGA TGCGCAGGCG GCTGAGCTTC ACCCTGGAGC GGCTGTCCAG CCGATTGCAG 
CGCAGTGGCA GCAGCGAGGA CTCGGGGGGC GCGTCGGGCC GCAGCACGCC GCTGTTCGGA 
CGGCTTCGCA GGGCCACGTC CGAGGGCGAG AGTCTGCGGC GCCTTGGCCT TCCGCACAAC 
CAGTTGGCCG CCCAGGCCGG CGCCACCACG CCTTCCGCCG AGTCCCTGGG CTCCGAGGCC 
AGCGCCACGT CGGGCTCCTC AGCCCCAGGG GAAAGCCGAA GCCGGCTCCG CTGGGGCTTC 
TCTCGGCCGC GGAAGGACAA GGGGTTATCG CCACCAAACC TCTCTGCCAG CGTCCAGGAG 
GAGTTGGGTC ACCAGTACGT GCGCAGTGAG TCAGACTTCC CCCCAGTCTT CCACATCAAA 
CTCAAGGACC AGGTGCTGCT GGAGGGGGAG GCAGCCACCC TGCTCTGCCT GCCAGCGGCC 
TGCCCTGCAC CGCACATCTC CTGGATGAAA GACAAGAAGT CCTTGAGGTC AGAGCCCTCA 
GTGATCATCG TGTCCTGCAA AGATGGGCGG CAGCTGCTCA GCATCCCCCG GGCGGGCAAG 
CGGCACGCCG GTCTCTATGA GTGCTCGGCC ACCAACGTAC TGGGCAGCAT CACCAGCTCC 
TGTACCGTGG CTGTGGCCCG AGTCCCAGGA AAGCTAGCTC CTCCAGAGGT ACCCCAGACC 
TACCAGGACA CGGCGCTGGT GCTGTGGAAG CCGGGAGACA GCCGGGCACC TTGCACGTAT 
ACGCTGGAGC GGCGAGTGGA TGGGGAGTCT GTGTGGCACC CTGTGAGCTC AGGCATCCCC 
GACTGTTACT ACAACGTGAC CCACCTGCCA GTTGGCGTGA CTGTGAGGTT CCGTGTGGCC 
TGTGCCAACC GTGCTGGGCA GGGGCCCTTC AGCAACTCTT CTGAGAAGGT CTTTGTCAGG 
GGTACTCAAG ATTCTTCAGC TGTGCCATCT GCTGCCCACC AAGAGGCCCC TGTCACCTCA 
AGGCCAGCCA GGGCCCGGCC TCCTGACTCT CCTACCTCAC TGGCCCCACC CCTAGCTCCT 
GCTGCCCCCA CACCCCCGTC AGTCACTGTC AGCCCCTCAT CTCCCCCCAC ACCTCCTAGC 
CAGGCCTTGT CCTCGCTCAA GGCTGTGGGT CCACCACCCC AAACCCCTCC ACGAAGACAC 
AGGGGCCTGC AGGCTGCCCG GCCAGCGGAG CCCACCCTAC CCAGTACCCA CGTCACCCCA 
AGTGAGCCCA AGCCTTTCGT CCTTGACACT GGGACCCCGA TCCCAGCCTC CACTCCTCAA 
GGGGTTAAAC CAGTGTCTTC CTCTACTCCT GTGTATGTGG TGACTTCCTT TGTGTCTGCA 
CCACCAGCCC CTGAGCCCCC AGCCCCTGAG CCCCCTCCTG AGCCTACCAA GGTGACTGTG 
CAGAGCCTCA GCCCGGCCAA GGAGGTGGTC AGCTCCCCTG GGAGCAGTCC CCGAAGCTCT 
CCCAGGCCTG AGGGTACCAC TCTTCGACAG GGTCCCCCTC AGAAACCCTA CACCTTCCTG 
GAGGAGAAAG CCAGGGGCCG CTTTGGTGTT GTGCGAGCGT GCCGGGAGAA TGCCACGGGG 
CGAACGTTCG TGGCCAAGAT CGTGCCCTAT GCTGCCGAGG GCAAGCGGCG GGTCCTGCAG 
GAGTACGAGG TGCTGCGGAC CCTGCACCAC GAGCGGATCA TGTCCCTGCA CGAGGCCTAC 
ATCACCCCTC GGTACCTCGT GCTCATTGCT GAGAGCTGTG GCAACCGGGA ACTCCTCTGT 
GGGCTCAGTG ACAGGTTCCG GTATTCTGAG GATGACGTGG CCACTTACAT GGTGCAGCTG 
CTACAAGGCC TGGACTACCT CCACGGCCAC CACGTGCTCC ACCTAGACAT CAAGCCAGAC 
AACCTGCTGC TGGCCCCTGA CAATGCCCTC AAGATTGTGG ACTTTGGCAG TGCCCAGCCC 
TACAACCCCC AGGCCCTTAG GCCCCTTGGC CACCGCACGG GCACGCTGGA GTTCATGGCT 
CCGGAGATGG TGAAGGGAGA ACCCATCGGC TCTGCCACGG ACATCTGGGG AGCGGGTGTG 
CTCACTTACA TTATGCTCAG TGGACGCTCC CCGTTCTATG AGCCAGACCC CCAGGAAACG 
GAGGCTCGGA TTGTGGGGGG CCGCTTTGAT GCCTTCCAGC TGTACCCCAA TACATCCCAG 
AGCGCCACCC TCTTCTTGCG AAAGGTTCTC TCTGTACATC CCTGGAGCCG GCCCTCCCTG 
CAGGACTGCC TGGCCCACCC ATGGTTGCAG GACGCCTACC TGATGAAGCT GCGCCGCCAG 
ACGCTCACCT TCACCACCAA CCGGCTCAAG GAGTTCCTGG GCGAGCAGCG GCGGCGCCGG 
GCTGAGGCTG CCACCCGCCA CAAGGTGCTG CTGCGCTCCT ACCCTGGCGG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712256.1
CDS	3412..10668
Translation	MKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPTDVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARFDCKISGTPPPVVTWTHFGCPMEESENLRLRQDGGLHSLHIAHVGSEDEGLYAVSAVNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEQGELERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDVHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPQVVAVTGRMVTLTWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPGWAATGLRKGVQHIFRVLSTTVKSSSKPSPPSEPVQLLEHGPTLEEAPAMLDKPDIVYVVEGQPASVTVTFNHVEAQVVWRSCRGALLEARAGVYELSQPDDDQYCLRICRVSRRDMGALTCTARNRHGTQTCSVTLELAEAPRFESIMEDVEVGAGETARFAVVVEGKPLPDIMWYKDEVLLTESSHVSFVYEENECSLVVLSTGAQDGGVYTCTAQNLAGEVSCKAELAVHSAQTAMEVEGVGEDEDHRGRRLSDFYDIHQEIGRGAFSYLRRIVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHDCVLYFHEAFERRRGLVIVTELCTEELLERIARKPTVCESEIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTQAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGELRRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQPPAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPSEPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEAKFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAVGEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAESLGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPHISWMKDKKSLRSEPSVIIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARVPGKLAPPEVPQTYQDTALVLWKPGDSRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTHLPVGVTVRFRVACANRAGQGPFSNSSEKVFVRGTQDSSAVPSAAHQEAPVTSRPARARPPDSPTSLAPPLAPAAPTPPSVTVSPSSPPTPPSQALSSLKAVGPPPQTPPRRHRGLQAARPAEPTLPSTHVTPSEPKPFVLDTGTPIPASTPQGVKPVSSSTPVYVVTSFVSAPPAPEPPAPEPPPEPTKVTVQSLSPAKEVVSSPGSSPRSSPRPEGTTLRQGPPQKPYTFLEEKARGRFGVVRACRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERIMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYMVQLLQGLDYLHGHHVLHLDIKPDNLLLAPDNALKIVDFGSAQPYNPQALRPLGHRTGTLEFMAPEMVKGEPIGSATDIWGAGVLTYIMLSGRSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGGP

Target ORF information:

RefSeq Version	XM_006712193.3
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712193.3

ORF Insert Sequence:

ATGAAGCCCA GTCCCAGCCA GAACCGCCGT TCTTCTGACA CTGGCTCCAA GGCACCCCCC 
ACCTTCAAGG TCTCACTTAT GGACCAGTCA GTAAGAGAAG GCCAAGATGT CATCATGAGC 
ATCCGCGTGC AGGGGGAGCC CAAGCCTGTG GTCTCCTGGC TGAGAAACCG CCAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCGGAGGAG GCTGAGGGTG GGCTGTGCCG GCTGCGGATC 
CTGGCTGCAG AGCGTGGCGA TGCTGGTTTC TACACTTGCA AAGCGGTCAA TGAGTATGGT 
GCTCGGCAGT GCGAGGCCCG CTTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCGG GGCCCACTGA CGTGGAGGTG GATTGGCTGT GCCGTGGCCG CCTGCTGCAG 
CCTGCACTGC TCAAATGCAA GATGCATTTC GATGGCCGCA AATGCAAGCT GCTACTTACA 
TCTGTACATG AGGACGACAG TGGCGTCTAC ACCTGCAAGC TCAGCACGGC CAAAGATGAG 
CTGACCTGCA GTGCCCGGCT GACCGTGCGG CCCTCGTTGG CACCCCTGTT CACACGGCTG 
CTGGAAGATG TGGAGGTGTT GGAGGGCCGA GCTGCCCGTT TCGACTGCAA GATCAGTGGC 
ACCCCGCCCC CTGTTGTTAC CTGGACTCAT TTTGGCTGCC CCATGGAGGA GAGTGAGAAC 
TTGCGGCTGC GGCAGGACGG GGGTCTGCAC TCACTGCACA TTGCCCATGT GGGCAGCGAG 
GACGAGGGGC TCTATGCGGT CAGTGCTGTT AACACCCATG GCCAGGCCCA CTGCTCAGCC 
CAGCTGTATG TAGAAGAGCC CCGGACAGCC GCCTCAGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCCA TTCCCGAGGA GCCAGAGCAG GGTGAGCTGG AGCGGCTGTC CATTCCTGAC 
TTCCTGCGGC CACTGCAGGA CCTGGAGGTG GGACTGGCCA AGGAGGCCAT GCTAGAGTGC 
CAGGTGACCG GCCTGCCCTA CCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATCCAG 
AGCAGCGACG ACCGGCGCAT GACACAGTAC AGGGATGTCC ATCGCTTGGT GTTCCCTGCC 
GTGGGGCCTC AGCACGCCGG TGTCTACAAG AGCGTCATTG CCAACAAGCT GGGCAAAGCT 
GCCTGCTATG CCCACCTGTA TGTCACAGAT GTGGTCCCAG GCCCTCCAGA TGGCGCCCCG 
CAGGTGGTGG CTGTGACGGG GAGGATGGTC ACACTCACAT GGAACCCCCC CAGGAGTCTG 
GACATGGCCA TCGACCCGGA CTCCCTGACG TACACAGTGC AGCACCAGGT GCTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCAG GGTGGGCAGC CACAGGGCTG 
CGTAAGGGGG TCCAGCACAT CTTCCGGGTC CTCAGCACCA CTGTCAAGAG CAGCAGCAAG 
CCCTCACCCC CTTCTGAGCC TGTGCAGCTG CTGGAGCACG GCCCAACCCT GGAGGAGGCC 
CCTGCCATGC TGGACAAACC AGACATCGTG TATGTGGTGG AGGGACAGCC TGCCAGCGTC 
ACCGTCACAT TCAACCATGT GGAGGCCCAG GTCGTCTGGA GGAGCTGCCG AGGGGCCCTC 
CTAGAGGCAC GGGCCGGTGT GTACGAGCTG AGCCAGCCAG ATGATGACCA GTACTGTCTT 
CGGATCTGCC GGGTGAGCCG CCGGGACATG GGGGCCCTCA CCTGCACCGC CCGAAACCGT 
CACGGCACAC AGACCTGCTC GGTCACATTG GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC 
ATCATGGAGG ACGTGGAGGT GGGGGCTGGG GAAACTGCTC GCTTTGCGGT GGTGGTCGAG 
GGAAAACCAC TGCCGGACAT CATGTGGTAC AAGGACGAGG TGCTGCTGAC CGAGAGCAGC 
CATGTGAGCT TCGTGTACGA GGAGAATGAG TGCTCCCTGG TGGTGCTCAG CACGGGGGCC 
CAGGATGGAG GCGTCTACAC CTGCACCGCC CAGAACCTGG CGGGTGAGGT CTCCTGCAAA 
GCAGAGTTGG CTGTGCATTC AGCTCAGACA GCTATGGAGG TCGAGGGGGT CGGGGAGGAT 
GAGGACCATC GAGGAAGGAG ACTCAGCGAC TTTTATGACA TCCACCAGGA GATCGGCAGG 
GGTGCTTTCT CCTACTTGCG GCGCATAGTG GAGCGTAGCT CCGGCCTGGA GTTTGCGGCC 
AAGTTCATCC CCAGCCAGGC CAAGCCAAAG GCATCAGCGC GTCGGGAGGC CCGGCTGCTG 
GCCAGGCTCC AGCACGACTG TGTCCTCTAC TTCCATGAGG CCTTCGAGAG GCGCCGGGGA 
CTGGTCATTG TCACCGAGCT CTGCACAGAG GAGCTGCTGG AGCGAATCGC CAGGAAACCC 
ACCGTGTGTG AGTCTGAGAT CCGGGCCTAT ATGCGGCAGG TGCTAGAGGG AATACACTAC 
CTGCACCAGA GCCACGTGCT GCACCTCGAT GTCAAGCCTG AGAACCTGCT GGTGTGGGAT 
GGTGCTGCGG GCGAGCAGCA GGTGCGGATC TGTGACTTTG GGAATGCCCA GGAGCTGACT 
CCAGGAGAGC CCCAGTACTG CCAGTATGGC ACACCTGAGT TTGTAGCACC CGAGATTGTC 
AATCAGAGCC CCGTGTCTGG AGTCACTGAC ATCTGGCCTG TGGGTGTTGT TGCCTTCCTC 
TGTCTGACAG GAATCTCCCC GTTTGTTGGG GAAAATGACC GGACAACATT GATGAACATC 
CGAAACTACA ACGTGGCCTT CGAGGAGACC ACATTCCTGA GCCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGTT GGTGCAGGAC CGGCTGAGAC CTACCGCAGA AGAGACCCTA 
GAACATCCTT GGTTCAAAAC TCAGGCAAAG GGCGCAGAGG TGAGCACGGA TCACCTGAAG 
CTATTCCTCT CCCGGCGGAG GTGGCAGCGC TCCCAGATCA GCTACAAATG CCACCTGGTG 
CTGCGCCCCA TCCCCGAGCT GCTGCGGGCC CCCCCAGAGC GGGTGTGGGT GACCATGCCC 
AGAAGGCCAC CCCCCAGTGG GGGGCTCTCA TCCTCCTCGG ATTCTGAAGA GGAAGAGCTG 
GAAGAGCTGC CCTCAGTGCC CCGCCCACTG CAGCCCGAGT TCTCTGGCTC CCGGGTGTCC 
CTCACAGACA TTCCCACTGA GGATGAGGCC CTGGGGACCC CAGAGACTGG GGCTGCCACC 
CCCATGGACT GGCAGGAGCA GGGAAGGGCT CCCTCTCAGG ACCAGGAGGC TCCCAGCCCA 
GAGGCCCTCC CCTCCCCAGG CCAGGAGCCC GCAGCTGGGG CTAGCCCCAG GCGGGGAGAG 
CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC CTGCCCCGGG CCGGGCCGCG GGAGCTGGGC 
CGGGGCCTGC ACAAGGCGGC GTCTGTGGAG CTGCCGCAGC GCCGGAGCCC CAGCCCGGGA 
GCCACCCGCC TGGCCCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGCCAGC GGCTGCTGCG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGT 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCT CGGGACCCCC GGATGGCACG AGCTGCCTCC 
AGCGAGGCAG CGCCCCACCA CCAGCCCCCA CTCGAGAACC GGGGCCTGCA AAAGAGCAGC 
AGCTTCTCCC AGGGTGAGGC GGAGCCCCGG GGCCGGCACC GCCGAGCGGG GGCGCCCCTC 
GAGATCCCCG TGGCCAGGCT TGGGGCCCGT AGGCTACAGG AGTCTCCTTC CCTGTCTGCC 
CTCAGCGAGG CCCAGCCATC CAGCCCTGCA CGGCCCAGCG CCCCCAAACC CAGTACCCCT 
AAGTCTGCAG AACCTTCTGC CACCACACCT AGTGATGCTC CGCAGCCCCC CGCACCCCAG 
CCTGCCCAAG ACAAGGCTCC AGAGCCCAGG CCAGAACCAG TCCGAGCCTC CAAGCCTGCA 
CCACCCCCCC AGGCCCTGCA AACCCTAGCG CTGCCCCTCA CACCCTATGC TCAGATCATT 
CAGTCCCTCC AGCTGTCAGG CCACGCCCAG GGCCCCTCGC AGGGCCCTGC CGCGCCGCCT 
TCAGAGCCCA AGCCCCACGC TGCTGTCTTT GCCAGGGTGG CCTCCCCACC TCCGGGAGCC 
CCCGAGAAGC GCGTGCCCTC AGCCGGGGGT CCCCCGGTGC TAGCCGAGAA AGCCCGAGTT 
CCCACGGTGC CCCCCAGGCC AGGCAGCAGT CTCAGTAGCA GCATCGAAAA CTTGGAGTCG 
GAGGCCGTGT TCGAGGCCAA GTTCAAGCGC AGCCGCGAGT CGCCCCTGTC GCTGGGGCTG 
CGGCTGCTGA GCCGTTCGCG CTCGGAGGAG CGCGGCCCCT TCCGTGGGGC CGAGGAGGAG 
GATGGCATAT ACCGGCCCAG CCCGGCGGGG ACCCCGCTGG AGCTGGTGCG ACGGCCTGAG 
CGCTCACGCT CGGTGCAGGA CCTCAGGGCT GTCGGAGAGC CTGGCCTCGT CCGCCGCCTC 
TCGCTGTCAC TGTCCCAGCG GCTGCGGCGG ACCCCTCCCG CGCAGCGCCA CCCGGCCTGG 
GAGGCCCGCG GCGGGGACGG AGAGAGCTCG GAGGGCGGGA GCTCGGCGCG GGGCTCCCCG 
GTGCTGGCGA TGCGCAGGCG GCTGAGCTTC ACCCTGGAGC GGCTGTCCAG CCGATTGCAG 
CGCAGTGGCA GCAGCGAGGA CTCGGGGGGC GCGTCGGGCC GCAGCACGCC GCTGTTCGGA 
CGGCTTCGCA GGGCCACGTC CGAGGGCGAG AGTCTGCGGC GCCTTGGCCT TCCGCACAAC 
CAGTTGGCCG CCCAGGCCGG CGCCACCACG CCTTCCGCCG AGTCCCTGGG CTCCGAGGCC 
AGCGCCACGT CGGGCTCCTC AGCCCCAGGG GAAAGCCGAA GCCGGCTCCG CTGGGGCTTC 
TCTCGGCCGC GGAAGGACAA GGGGTTATCG CCACCAAACC TCTCTGCCAG CGTCCAGGAG 
GAGTTGGGTC ACCAGTACGT GCGCAGTGAG TCAGACTTCC CCCCAGTCTT CCACATCAAA 
CTCAAGGACC AGGTGCTGCT GGAGGGGGAG GCAGCCACCC TGCTCTGCCT GCCAGCGGCC 
TGCCCTGCAC CGCACATCTC CTGGATGAAA GACAAGAAGT CCTTGAGGTC AGAGCCCTCA 
GTGATCATCG TGTCCTGCAA AGATGGGCGG CAGCTGCTCA GCATCCCCCG GGCGGGCAAG 
CGGCACGCCG GTCTCTATGA GTGCTCGGCC ACCAACGTAC TGGGCAGCAT CACCAGCTCC 
TGTACCGTGG CTGTGGCCCG AGTCCCAGGA AAGCTAGCTC CTCCAGAGGT ACCCCAGACC 
TACCAGGACA CGGCGCTGGT GCTGTGGAAG CCGGGAGACA GCCGGGCACC TTGCACGTAT 
ACGCTGGAGC GGCGAGTGGA TGGGGAGTCT GTGTGGCACC CTGTGAGCTC AGGCATCCCC 
GACTGTTACT ACAACGTGAC CCACCTGCCA GTTGGCGTGA CTGTGAGGTT CCGTGTGGCC 
TGTGCCAACC GTGCTGGGCA GGGGCCCTTC AGCAACTCTT CTGAGAAGGT CTTTGTCAGG 
GGTACTCAAG ATTCTTCAGC TGTGCCATCT GCTGCCCACC AAGAGGCCCC TGTCACCTCA 
AGGCCAGCCA GGGCCCGGCC TCCTGACTCT CCTACCTCAC TGGCCCCACC CCTAGCTCCT 
GCTGCCCCCA CACCCCCGTC AGTCACTGTC AGCCCCTCAT CTCCCCCCAC ACCTCCTAGC 
CAGGCCTTGT CCTCGCTCAA GGCTGTGGGT CCACCACCCC AAACCCCTCC ACGAAGACAC 
AGGGGCCTGC AGGCTGCCCG GCCAGCGGAG CCCACCCTAC CCAGTACCCA CGTCACCCCA 
AGTGAGCCCA AGCCTTTCGT CCTTGACACT GGGACCCCGA TCCCAGCCTC CACTCCTCAA 
GGGGTTAAAC CAGTGTCTTC CTCTACTCCT GTGTATGTGG TGACTTCCTT TGTGTCTGCA 
CCACCAGCCC CTGAGCCCCC AGCCCCTGAG CCCCCTCCTG AGCCTACCAA GGTGACTGTG 
CAGAGCCTCA GCCCGGCCAA GGAGGTGGTC AGCTCCCCTG GGAGCAGTCC CCGAAGCTCT 
CCCAGGCCTG AGGGTACCAC TCTTCGACAG GGTCCCCCTC AGAAACCCTA CACCTTCCTG 
GAGGAGAAAG CCAGGGGCCG CTTTGGTGTT GTGCGAGCGT GCCGGGAGAA TGCCACGGGG 
CGAACGTTCG TGGCCAAGAT CGTGCCCTAT GCTGCCGAGG GCAAGCGGCG GGTCCTGCAG 
GAGTACGAGG TGCTGCGGAC CCTGCACCAC GAGCGGATCA TGTCCCTGCA CGAGGCCTAC 
ATCACCCCTC GGTACCTCGT GCTCATTGCT GAGAGCTGTG GCAACCGGGA ACTCCTCTGT 
GGGCTCAGTG ACAGGTTCCG GTATTCTGAG GATGACGTGG CCACTTACAT GGTGCAGCTG 
CTACAAGGCC TGGACTACCT CCACGGCCAC CACGTGCTCC ACCTAGACAT CAAGCCAGAC 
AACCTGCTGC TGGCCCCTGA CAATGCCCTC AAGATTGTGG ACTTTGGCAG TGCCCAGCCC 
TACAACCCCC AGGCCCTTAG GCCCCTTGGC CACCGCACGG GCACGCTGGA GTTCATGGCT 
CCGGAGATGG TGAAGGGAGA ACCCATCGGC TCTGCCACGG ACATCTGGGG AGCGGGTGTG 
CTCACTTACA TTATGCTCAG TGGACGCTCC CCGTTCTATG AGCCAGACCC CCAGGAAACG 
GAGGCTCGGA TTGTGGGGGG CCGCTTTGAT GCCTTCCAGC TGTACCCCAA TACATCCCAG 
AGCGCCACCC TCTTCTTGCG AAAGGTTCTC TCTGTACATC CCTGGAGCCG GCCCTCCCTG 
CAGGACTGCC TGGCCCACCC ATGGTTGCAG GACGCCTACC TGATGAAGCT GCGCCGCCAG 
ACGCTCACCT TCACCACCAA CCGGCTCAAG GAGTTCCTGG GCGAGCAGCG GCGGCGCCGG 
GCTGAGGCTG CCACCCGCCA CAAGGTGCTG CTGCGCTCCT ACCCTGGCGG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_017003158.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858647.1 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X4, mRNA.

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CloneID	OHu35758
Clone ID Related Accession (Same CDS sequence)	XM_017003158.1 , XM_005246241.1 , XM_006712193.3 , XM_017003158.2 , XM_005246240.2
Accession Version	XM_017003158.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017003158.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCCA GTCCCAGCCA GAACCGCCGT TCTTCTGACA CTGGCTCCAA GGCACCCCCC 
ACCTTCAAGG TCTCACTTAT GGACCAGTCA GTAAGAGAAG GCCAAGATGT CATCATGAGC 
ATCCGCGTGC AGGGGGAGCC CAAGCCTGTG GTCTCCTGGC TGAGAAACCG CCAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCGGAGGAG GCTGAGGGTG GGCTGTGCCG GCTGCGGATC 
CTGGCTGCAG AGCGTGGCGA TGCTGGTTTC TACACTTGCA AAGCGGTCAA TGAGTATGGT 
GCTCGGCAGT GCGAGGCCCG CTTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCGG GGCCCACTGA CGTGGAGGTG GATTGGCTGT GCCGTGGCCG CCTGCTGCAG 
CCTGCACTGC TCAAATGCAA GATGCATTTC GATGGCCGCA AATGCAAGCT GCTACTTACA 
TCTGTACATG AGGACGACAG TGGCGTCTAC ACCTGCAAGC TCAGCACGGC CAAAGATGAG 
CTGACCTGCA GTGCCCGGCT GACCGTGCGG CCCTCGTTGG CACCCCTGTT CACACGGCTG 
CTGGAAGATG TGGAGGTGTT GGAGGGCCGA GCTGCCCGTT TCGACTGCAA GATCAGTGGC 
ACCCCGCCCC CTGTTGTTAC CTGGACTCAT TTTGGCTGCC CCATGGAGGA GAGTGAGAAC 
TTGCGGCTGC GGCAGGACGG GGGTCTGCAC TCACTGCACA TTGCCCATGT GGGCAGCGAG 
GACGAGGGGC TCTATGCGGT CAGTGCTGTT AACACCCATG GCCAGGCCCA CTGCTCAGCC 
CAGCTGTATG TAGAAGAGCC CCGGACAGCC GCCTCAGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCCA TTCCCGAGGA GCCAGAGCAG GGTGAGCTGG AGCGGCTGTC CATTCCTGAC 
TTCCTGCGGC CACTGCAGGA CCTGGAGGTG GGACTGGCCA AGGAGGCCAT GCTAGAGTGC 
CAGGTGACCG GCCTGCCCTA CCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATCCAG 
AGCAGCGACG ACCGGCGCAT GACACAGTAC AGGGATGTCC ATCGCTTGGT GTTCCCTGCC 
GTGGGGCCTC AGCACGCCGG TGTCTACAAG AGCGTCATTG CCAACAAGCT GGGCAAAGCT 
GCCTGCTATG CCCACCTGTA TGTCACAGAT GTGGTCCCAG GCCCTCCAGA TGGCGCCCCG 
CAGGTGGTGG CTGTGACGGG GAGGATGGTC ACACTCACAT GGAACCCCCC CAGGAGTCTG 
GACATGGCCA TCGACCCGGA CTCCCTGACG TACACAGTGC AGCACCAGGT GCTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCAG GGTGGGCAGC CACAGGGCTG 
CGTAAGGGGG TCCAGCACAT CTTCCGGGTC CTCAGCACCA CTGTCAAGAG CAGCAGCAAG 
CCCTCACCCC CTTCTGAGCC TGTGCAGCTG CTGGAGCACG GCCCAACCCT GGAGGAGGCC 
CCTGCCATGC TGGACAAACC AGACATCGTG TATGTGGTGG AGGGACAGCC TGCCAGCGTC 
ACCGTCACAT TCAACCATGT GGAGGCCCAG GTCGTCTGGA GGAGCTGCCG AGGGGCCCTC 
CTAGAGGCAC GGGCCGGTGT GTACGAGCTG AGCCAGCCAG ATGATGACCA GTACTGTCTT 
CGGATCTGCC GGGTGAGCCG CCGGGACATG GGGGCCCTCA CCTGCACCGC CCGAAACCGT 
CACGGCACAC AGACCTGCTC GGTCACATTG GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC 
ATCATGGAGG ACGTGGAGGT GGGGGCTGGG GAAACTGCTC GCTTTGCGGT GGTGGTCGAG 
GGAAAACCAC TGCCGGACAT CATGTGGTAC AAGGACGAGG TGCTGCTGAC CGAGAGCAGC 
CATGTGAGCT TCGTGTACGA GGAGAATGAG TGCTCCCTGG TGGTGCTCAG CACGGGGGCC 
CAGGATGGAG GCGTCTACAC CTGCACCGCC CAGAACCTGG CGGGTGAGGT CTCCTGCAAA 
GCAGAGTTGG CTGTGCATTC AGCTCAGACA GCTATGGAGG TCGAGGGGGT CGGGGAGGAT 
GAGGACCATC GAGGAAGGAG ACTCAGCGAC TTTTATGACA TCCACCAGGA GATCGGCAGG 
GGTGCTTTCT CCTACTTGCG GCGCATAGTG GAGCGTAGCT CCGGCCTGGA GTTTGCGGCC 
AAGTTCATCC CCAGCCAGGC CAAGCCAAAG GCATCAGCGC GTCGGGAGGC CCGGCTGCTG 
GCCAGGCTCC AGCACGACTG TGTCCTCTAC TTCCATGAGG CCTTCGAGAG GCGCCGGGGA 
CTGGTCATTG TCACCGAGCT CTGCACAGAG GAGCTGCTGG AGCGAATCGC CAGGAAACCC 
ACCGTGTGTG AGTCTGAGAT CCGGGCCTAT ATGCGGCAGG TGCTAGAGGG AATACACTAC 
CTGCACCAGA GCCACGTGCT GCACCTCGAT GTCAAGCCTG AGAACCTGCT GGTGTGGGAT 
GGTGCTGCGG GCGAGCAGCA GGTGCGGATC TGTGACTTTG GGAATGCCCA GGAGCTGACT 
CCAGGAGAGC CCCAGTACTG CCAGTATGGC ACACCTGAGT TTGTAGCACC CGAGATTGTC 
AATCAGAGCC CCGTGTCTGG AGTCACTGAC ATCTGGCCTG TGGGTGTTGT TGCCTTCCTC 
TGTCTGACAG GAATCTCCCC GTTTGTTGGG GAAAATGACC GGACAACATT GATGAACATC 
CGAAACTACA ACGTGGCCTT CGAGGAGACC ACATTCCTGA GCCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGTT GGTGCAGGAC CGGCTGAGAC CTACCGCAGA AGAGACCCTA 
GAACATCCTT GGTTCAAAAC TCAGGCAAAG GGCGCAGAGG TGAGCACGGA TCACCTGAAG 
CTATTCCTCT CCCGGCGGAG GTGGCAGCGC TCCCAGATCA GCTACAAATG CCACCTGGTG 
CTGCGCCCCA TCCCCGAGCT GCTGCGGGCC CCCCCAGAGC GGGTGTGGGT GACCATGCCC 
AGAAGGCCAC CCCCCAGTGG GGGGCTCTCA TCCTCCTCGG ATTCTGAAGA GGAAGAGCTG 
GAAGAGCTGC CCTCAGTGCC CCGCCCACTG CAGCCCGAGT TCTCTGGCTC CCGGGTGTCC 
CTCACAGACA TTCCCACTGA GGATGAGGCC CTGGGGACCC CAGAGACTGG GGCTGCCACC 
CCCATGGACT GGCAGGAGCA GGGAAGGGCT CCCTCTCAGG ACCAGGAGGC TCCCAGCCCA 
GAGGCCCTCC CCTCCCCAGG CCAGGAGCCC GCAGCTGGGG CTAGCCCCAG GCGGGGAGAG 
CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC CTGCCCCGGG CCGGGCCGCG GGAGCTGGGC 
CGGGGCCTGC ACAAGGCGGC GTCTGTGGAG CTGCCGCAGC GCCGGAGCCC CAGCCCGGGA 
GCCACCCGCC TGGCCCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGCCAGC GGCTGCTGCG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGT 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCT CGGGACCCCC GGATGGCACG AGCTGCCTCC 
AGCGAGGCAG CGCCCCACCA CCAGCCCCCA CTCGAGAACC GGGGCCTGCA AAAGAGCAGC 
AGCTTCTCCC AGGGTGAGGC GGAGCCCCGG GGCCGGCACC GCCGAGCGGG GGCGCCCCTC 
GAGATCCCCG TGGCCAGGCT TGGGGCCCGT AGGCTACAGG AGTCTCCTTC CCTGTCTGCC 
CTCAGCGAGG CCCAGCCATC CAGCCCTGCA CGGCCCAGCG CCCCCAAACC CAGTACCCCT 
AAGTCTGCAG AACCTTCTGC CACCACACCT AGTGATGCTC CGCAGCCCCC CGCACCCCAG 
CCTGCCCAAG ACAAGGCTCC AGAGCCCAGG CCAGAACCAG TCCGAGCCTC CAAGCCTGCA 
CCACCCCCCC AGGCCCTGCA AACCCTAGCG CTGCCCCTCA CACCCTATGC TCAGATCATT 
CAGTCCCTCC AGCTGTCAGG CCACGCCCAG GGCCCCTCGC AGGGCCCTGC CGCGCCGCCT 
TCAGAGCCCA AGCCCCACGC TGCTGTCTTT GCCAGGGTGG CCTCCCCACC TCCGGGAGCC 
CCCGAGAAGC GCGTGCCCTC AGCCGGGGGT CCCCCGGTGC TAGCCGAGAA AGCCCGAGTT 
CCCACGGTGC CCCCCAGGCC AGGCAGCAGT CTCAGTAGCA GCATCGAAAA CTTGGAGTCG 
GAGGCCGTGT TCGAGGCCAA GTTCAAGCGC AGCCGCGAGT CGCCCCTGTC GCTGGGGCTG 
CGGCTGCTGA GCCGTTCGCG CTCGGAGGAG CGCGGCCCCT TCCGTGGGGC CGAGGAGGAG 
GATGGCATAT ACCGGCCCAG CCCGGCGGGG ACCCCGCTGG AGCTGGTGCG ACGGCCTGAG 
CGCTCACGCT CGGTGCAGGA CCTCAGGGCT GTCGGAGAGC CTGGCCTCGT CCGCCGCCTC 
TCGCTGTCAC TGTCCCAGCG GCTGCGGCGG ACCCCTCCCG CGCAGCGCCA CCCGGCCTGG 
GAGGCCCGCG GCGGGGACGG AGAGAGCTCG GAGGGCGGGA GCTCGGCGCG GGGCTCCCCG 
GTGCTGGCGA TGCGCAGGCG GCTGAGCTTC ACCCTGGAGC GGCTGTCCAG CCGATTGCAG 
CGCAGTGGCA GCAGCGAGGA CTCGGGGGGC GCGTCGGGCC GCAGCACGCC GCTGTTCGGA 
CGGCTTCGCA GGGCCACGTC CGAGGGCGAG AGTCTGCGGC GCCTTGGCCT TCCGCACAAC 
CAGTTGGCCG CCCAGGCCGG CGCCACCACG CCTTCCGCCG AGTCCCTGGG CTCCGAGGCC 
AGCGCCACGT CGGGCTCCTC AGCCCCAGGG GAAAGCCGAA GCCGGCTCCG CTGGGGCTTC 
TCTCGGCCGC GGAAGGACAA GGGGTTATCG CCACCAAACC TCTCTGCCAG CGTCCAGGAG 
GAGTTGGGTC ACCAGTACGT GCGCAGTGAG TCAGACTTCC CCCCAGTCTT CCACATCAAA 
CTCAAGGACC AGGTGCTGCT GGAGGGGGAG GCAGCCACCC TGCTCTGCCT GCCAGCGGCC 
TGCCCTGCAC CGCACATCTC CTGGATGAAA GACAAGAAGT CCTTGAGGTC AGAGCCCTCA 
GTGATCATCG TGTCCTGCAA AGATGGGCGG CAGCTGCTCA GCATCCCCCG GGCGGGCAAG 
CGGCACGCCG GTCTCTATGA GTGCTCGGCC ACCAACGTAC TGGGCAGCAT CACCAGCTCC 
TGTACCGTGG CTGTGGCCCG AGTCCCAGGA AAGCTAGCTC CTCCAGAGGT ACCCCAGACC 
TACCAGGACA CGGCGCTGGT GCTGTGGAAG CCGGGAGACA GCCGGGCACC TTGCACGTAT 
ACGCTGGAGC GGCGAGTGGA TGGGGAGTCT GTGTGGCACC CTGTGAGCTC AGGCATCCCC 
GACTGTTACT ACAACGTGAC CCACCTGCCA GTTGGCGTGA CTGTGAGGTT CCGTGTGGCC 
TGTGCCAACC GTGCTGGGCA GGGGCCCTTC AGCAACTCTT CTGAGAAGGT CTTTGTCAGG 
GGTACTCAAG ATTCTTCAGC TGTGCCATCT GCTGCCCACC AAGAGGCCCC TGTCACCTCA 
AGGCCAGCCA GGGCCCGGCC TCCTGACTCT CCTACCTCAC TGGCCCCACC CCTAGCTCCT 
GCTGCCCCCA CACCCCCGTC AGTCACTGTC AGCCCCTCAT CTCCCCCCAC ACCTCCTAGC 
CAGGCCTTGT CCTCGCTCAA GGCTGTGGGT CCACCACCCC AAACCCCTCC ACGAAGACAC 
AGGGGCCTGC AGGCTGCCCG GCCAGCGGAG CCCACCCTAC CCAGTACCCA CGTCACCCCA 
AGTGAGCCCA AGCCTTTCGT CCTTGACACT GGGACCCCGA TCCCAGCCTC CACTCCTCAA 
GGGGTTAAAC CAGTGTCTTC CTCTACTCCT GTGTATGTGG TGACTTCCTT TGTGTCTGCA 
CCACCAGCCC CTGAGCCCCC AGCCCCTGAG CCCCCTCCTG AGCCTACCAA GGTGACTGTG 
CAGAGCCTCA GCCCGGCCAA GGAGGTGGTC AGCTCCCCTG GGAGCAGTCC CCGAAGCTCT 
CCCAGGCCTG AGGGTACCAC TCTTCGACAG GGTCCCCCTC AGAAACCCTA CACCTTCCTG 
GAGGAGAAAG CCAGGGGCCG CTTTGGTGTT GTGCGAGCGT GCCGGGAGAA TGCCACGGGG 
CGAACGTTCG TGGCCAAGAT CGTGCCCTAT GCTGCCGAGG GCAAGCGGCG GGTCCTGCAG 
GAGTACGAGG TGCTGCGGAC CCTGCACCAC GAGCGGATCA TGTCCCTGCA CGAGGCCTAC 
ATCACCCCTC GGTACCTCGT GCTCATTGCT GAGAGCTGTG GCAACCGGGA ACTCCTCTGT 
GGGCTCAGTG ACAGGTTCCG GTATTCTGAG GATGACGTGG CCACTTACAT GGTGCAGCTG 
CTACAAGGCC TGGACTACCT CCACGGCCAC CACGTGCTCC ACCTAGACAT CAAGCCAGAC 
AACCTGCTGC TGGCCCCTGA CAATGCCCTC AAGATTGTGG ACTTTGGCAG TGCCCAGCCC 
TACAACCCCC AGGCCCTTAG GCCCCTTGGC CACCGCACGG GCACGCTGGA GTTCATGGCT 
CCGGAGATGG TGAAGGGAGA ACCCATCGGC TCTGCCACGG ACATCTGGGG AGCGGGTGTG 
CTCACTTACA TTATGCTCAG TGGACGCTCC CCGTTCTATG AGCCAGACCC CCAGGAAACG 
GAGGCTCGGA TTGTGGGGGG CCGCTTTGAT GCCTTCCAGC TGTACCCCAA TACATCCCAG 
AGCGCCACCC TCTTCTTGCG AAAGGTTCTC TCTGTACATC CCTGGAGCCG GCCCTCCCTG 
CAGGACTGCC TGGCCCACCC ATGGTTGCAG GACGCCTACC TGATGAAGCT GCGCCGCCAG 
ACGCTCACCT TCACCACCAA CCGGCTCAAG GAGTTCCTGG GCGAGCAGCG GCGGCGCCGG 
GCTGAGGCTG CCACCCGCCA CAAGGTGCTG CTGCGCTCCT ACCCTGGCGG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858647.1
CDS	1657..8913
Translation	MKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPTDVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARFDCKISGTPPPVVTWTHFGCPMEESENLRLRQDGGLHSLHIAHVGSEDEGLYAVSAVNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEQGELERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDVHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPQVVAVTGRMVTLTWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPGWAATGLRKGVQHIFRVLSTTVKSSSKPSPPSEPVQLLEHGPTLEEAPAMLDKPDIVYVVEGQPASVTVTFNHVEAQVVWRSCRGALLEARAGVYELSQPDDDQYCLRICRVSRRDMGALTCTARNRHGTQTCSVTLELAEAPRFESIMEDVEVGAGETARFAVVVEGKPLPDIMWYKDEVLLTESSHVSFVYEENECSLVVLSTGAQDGGVYTCTAQNLAGEVSCKAELAVHSAQTAMEVEGVGEDEDHRGRRLSDFYDIHQEIGRGAFSYLRRIVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHDCVLYFHEAFERRRGLVIVTELCTEELLERIARKPTVCESEIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTQAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGELRRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQPPAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPSEPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEAKFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAVGEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAESLGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPHISWMKDKKSLRSEPSVIIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARVPGKLAPPEVPQTYQDTALVLWKPGDSRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTHLPVGVTVRFRVACANRAGQGPFSNSSEKVFVRGTQDSSAVPSAAHQEAPVTSRPARARPPDSPTSLAPPLAPAAPTPPSVTVSPSSPPTPPSQALSSLKAVGPPPQTPPRRHRGLQAARPAEPTLPSTHVTPSEPKPFVLDTGTPIPASTPQGVKPVSSSTPVYVVTSFVSAPPAPEPPAPEPPPEPTKVTVQSLSPAKEVVSSPGSSPRSSPRPEGTTLRQGPPQKPYTFLEEKARGRFGVVRACRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERIMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYMVQLLQGLDYLHGHHVLHLDIKPDNLLLAPDNALKIVDFGSAQPYNPQALRPLGHRTGTLEFMAPEMVKGEPIGSATDIWGAGVLTYIMLSGRSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGGP

Target ORF information:

RefSeq Version	XM_017003158.2
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003158.2

ORF Insert Sequence:

ATGAAGCCCA GTCCCAGCCA GAACCGCCGT TCTTCTGACA CTGGCTCCAA GGCACCCCCC 
ACCTTCAAGG TCTCACTTAT GGACCAGTCA GTAAGAGAAG GCCAAGATGT CATCATGAGC 
ATCCGCGTGC AGGGGGAGCC CAAGCCTGTG GTCTCCTGGC TGAGAAACCG CCAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCGGAGGAG GCTGAGGGTG GGCTGTGCCG GCTGCGGATC 
CTGGCTGCAG AGCGTGGCGA TGCTGGTTTC TACACTTGCA AAGCGGTCAA TGAGTATGGT 
GCTCGGCAGT GCGAGGCCCG CTTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCGG GGCCCACTGA CGTGGAGGTG GATTGGCTGT GCCGTGGCCG CCTGCTGCAG 
CCTGCACTGC TCAAATGCAA GATGCATTTC GATGGCCGCA AATGCAAGCT GCTACTTACA 
TCTGTACATG AGGACGACAG TGGCGTCTAC ACCTGCAAGC TCAGCACGGC CAAAGATGAG 
CTGACCTGCA GTGCCCGGCT GACCGTGCGG CCCTCGTTGG CACCCCTGTT CACACGGCTG 
CTGGAAGATG TGGAGGTGTT GGAGGGCCGA GCTGCCCGTT TCGACTGCAA GATCAGTGGC 
ACCCCGCCCC CTGTTGTTAC CTGGACTCAT TTTGGCTGCC CCATGGAGGA GAGTGAGAAC 
TTGCGGCTGC GGCAGGACGG GGGTCTGCAC TCACTGCACA TTGCCCATGT GGGCAGCGAG 
GACGAGGGGC TCTATGCGGT CAGTGCTGTT AACACCCATG GCCAGGCCCA CTGCTCAGCC 
CAGCTGTATG TAGAAGAGCC CCGGACAGCC GCCTCAGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCCA TTCCCGAGGA GCCAGAGCAG GGTGAGCTGG AGCGGCTGTC CATTCCTGAC 
TTCCTGCGGC CACTGCAGGA CCTGGAGGTG GGACTGGCCA AGGAGGCCAT GCTAGAGTGC 
CAGGTGACCG GCCTGCCCTA CCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATCCAG 
AGCAGCGACG ACCGGCGCAT GACACAGTAC AGGGATGTCC ATCGCTTGGT GTTCCCTGCC 
GTGGGGCCTC AGCACGCCGG TGTCTACAAG AGCGTCATTG CCAACAAGCT GGGCAAAGCT 
GCCTGCTATG CCCACCTGTA TGTCACAGAT GTGGTCCCAG GCCCTCCAGA TGGCGCCCCG 
CAGGTGGTGG CTGTGACGGG GAGGATGGTC ACACTCACAT GGAACCCCCC CAGGAGTCTG 
GACATGGCCA TCGACCCGGA CTCCCTGACG TACACAGTGC AGCACCAGGT GCTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCAG GGTGGGCAGC CACAGGGCTG 
CGTAAGGGGG TCCAGCACAT CTTCCGGGTC CTCAGCACCA CTGTCAAGAG CAGCAGCAAG 
CCCTCACCCC CTTCTGAGCC TGTGCAGCTG CTGGAGCACG GCCCAACCCT GGAGGAGGCC 
CCTGCCATGC TGGACAAACC AGACATCGTG TATGTGGTGG AGGGACAGCC TGCCAGCGTC 
ACCGTCACAT TCAACCATGT GGAGGCCCAG GTCGTCTGGA GGAGCTGCCG AGGGGCCCTC 
CTAGAGGCAC GGGCCGGTGT GTACGAGCTG AGCCAGCCAG ATGATGACCA GTACTGTCTT 
CGGATCTGCC GGGTGAGCCG CCGGGACATG GGGGCCCTCA CCTGCACCGC CCGAAACCGT 
CACGGCACAC AGACCTGCTC GGTCACATTG GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC 
ATCATGGAGG ACGTGGAGGT GGGGGCTGGG GAAACTGCTC GCTTTGCGGT GGTGGTCGAG 
GGAAAACCAC TGCCGGACAT CATGTGGTAC AAGGACGAGG TGCTGCTGAC CGAGAGCAGC 
CATGTGAGCT TCGTGTACGA GGAGAATGAG TGCTCCCTGG TGGTGCTCAG CACGGGGGCC 
CAGGATGGAG GCGTCTACAC CTGCACCGCC CAGAACCTGG CGGGTGAGGT CTCCTGCAAA 
GCAGAGTTGG CTGTGCATTC AGCTCAGACA GCTATGGAGG TCGAGGGGGT CGGGGAGGAT 
GAGGACCATC GAGGAAGGAG ACTCAGCGAC TTTTATGACA TCCACCAGGA GATCGGCAGG 
GGTGCTTTCT CCTACTTGCG GCGCATAGTG GAGCGTAGCT CCGGCCTGGA GTTTGCGGCC 
AAGTTCATCC CCAGCCAGGC CAAGCCAAAG GCATCAGCGC GTCGGGAGGC CCGGCTGCTG 
GCCAGGCTCC AGCACGACTG TGTCCTCTAC TTCCATGAGG CCTTCGAGAG GCGCCGGGGA 
CTGGTCATTG TCACCGAGCT CTGCACAGAG GAGCTGCTGG AGCGAATCGC CAGGAAACCC 
ACCGTGTGTG AGTCTGAGAT CCGGGCCTAT ATGCGGCAGG TGCTAGAGGG AATACACTAC 
CTGCACCAGA GCCACGTGCT GCACCTCGAT GTCAAGCCTG AGAACCTGCT GGTGTGGGAT 
GGTGCTGCGG GCGAGCAGCA GGTGCGGATC TGTGACTTTG GGAATGCCCA GGAGCTGACT 
CCAGGAGAGC CCCAGTACTG CCAGTATGGC ACACCTGAGT TTGTAGCACC CGAGATTGTC 
AATCAGAGCC CCGTGTCTGG AGTCACTGAC ATCTGGCCTG TGGGTGTTGT TGCCTTCCTC 
TGTCTGACAG GAATCTCCCC GTTTGTTGGG GAAAATGACC GGACAACATT GATGAACATC 
CGAAACTACA ACGTGGCCTT CGAGGAGACC ACATTCCTGA GCCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGTT GGTGCAGGAC CGGCTGAGAC CTACCGCAGA AGAGACCCTA 
GAACATCCTT GGTTCAAAAC TCAGGCAAAG GGCGCAGAGG TGAGCACGGA TCACCTGAAG 
CTATTCCTCT CCCGGCGGAG GTGGCAGCGC TCCCAGATCA GCTACAAATG CCACCTGGTG 
CTGCGCCCCA TCCCCGAGCT GCTGCGGGCC CCCCCAGAGC GGGTGTGGGT GACCATGCCC 
AGAAGGCCAC CCCCCAGTGG GGGGCTCTCA TCCTCCTCGG ATTCTGAAGA GGAAGAGCTG 
GAAGAGCTGC CCTCAGTGCC CCGCCCACTG CAGCCCGAGT TCTCTGGCTC CCGGGTGTCC 
CTCACAGACA TTCCCACTGA GGATGAGGCC CTGGGGACCC CAGAGACTGG GGCTGCCACC 
CCCATGGACT GGCAGGAGCA GGGAAGGGCT CCCTCTCAGG ACCAGGAGGC TCCCAGCCCA 
GAGGCCCTCC CCTCCCCAGG CCAGGAGCCC GCAGCTGGGG CTAGCCCCAG GCGGGGAGAG 
CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC CTGCCCCGGG CCGGGCCGCG GGAGCTGGGC 
CGGGGCCTGC ACAAGGCGGC GTCTGTGGAG CTGCCGCAGC GCCGGAGCCC CAGCCCGGGA 
GCCACCCGCC TGGCCCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGCCAGC GGCTGCTGCG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGT 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCT CGGGACCCCC GGATGGCACG AGCTGCCTCC 
AGCGAGGCAG CGCCCCACCA CCAGCCCCCA CTCGAGAACC GGGGCCTGCA AAAGAGCAGC 
AGCTTCTCCC AGGGTGAGGC GGAGCCCCGG GGCCGGCACC GCCGAGCGGG GGCGCCCCTC 
GAGATCCCCG TGGCCAGGCT TGGGGCCCGT AGGCTACAGG AGTCTCCTTC CCTGTCTGCC 
CTCAGCGAGG CCCAGCCATC CAGCCCTGCA CGGCCCAGCG CCCCCAAACC CAGTACCCCT 
AAGTCTGCAG AACCTTCTGC CACCACACCT AGTGATGCTC CGCAGCCCCC CGCACCCCAG 
CCTGCCCAAG ACAAGGCTCC AGAGCCCAGG CCAGAACCAG TCCGAGCCTC CAAGCCTGCA 
CCACCCCCCC AGGCCCTGCA AACCCTAGCG CTGCCCCTCA CACCCTATGC TCAGATCATT 
CAGTCCCTCC AGCTGTCAGG CCACGCCCAG GGCCCCTCGC AGGGCCCTGC CGCGCCGCCT 
TCAGAGCCCA AGCCCCACGC TGCTGTCTTT GCCAGGGTGG CCTCCCCACC TCCGGGAGCC 
CCCGAGAAGC GCGTGCCCTC AGCCGGGGGT CCCCCGGTGC TAGCCGAGAA AGCCCGAGTT 
CCCACGGTGC CCCCCAGGCC AGGCAGCAGT CTCAGTAGCA GCATCGAAAA CTTGGAGTCG 
GAGGCCGTGT TCGAGGCCAA GTTCAAGCGC AGCCGCGAGT CGCCCCTGTC GCTGGGGCTG 
CGGCTGCTGA GCCGTTCGCG CTCGGAGGAG CGCGGCCCCT TCCGTGGGGC CGAGGAGGAG 
GATGGCATAT ACCGGCCCAG CCCGGCGGGG ACCCCGCTGG AGCTGGTGCG ACGGCCTGAG 
CGCTCACGCT CGGTGCAGGA CCTCAGGGCT GTCGGAGAGC CTGGCCTCGT CCGCCGCCTC 
TCGCTGTCAC TGTCCCAGCG GCTGCGGCGG ACCCCTCCCG CGCAGCGCCA CCCGGCCTGG 
GAGGCCCGCG GCGGGGACGG AGAGAGCTCG GAGGGCGGGA GCTCGGCGCG GGGCTCCCCG 
GTGCTGGCGA TGCGCAGGCG GCTGAGCTTC ACCCTGGAGC GGCTGTCCAG CCGATTGCAG 
CGCAGTGGCA GCAGCGAGGA CTCGGGGGGC GCGTCGGGCC GCAGCACGCC GCTGTTCGGA 
CGGCTTCGCA GGGCCACGTC CGAGGGCGAG AGTCTGCGGC GCCTTGGCCT TCCGCACAAC 
CAGTTGGCCG CCCAGGCCGG CGCCACCACG CCTTCCGCCG AGTCCCTGGG CTCCGAGGCC 
AGCGCCACGT CGGGCTCCTC AGCCCCAGGG GAAAGCCGAA GCCGGCTCCG CTGGGGCTTC 
TCTCGGCCGC GGAAGGACAA GGGGTTATCG CCACCAAACC TCTCTGCCAG CGTCCAGGAG 
GAGTTGGGTC ACCAGTACGT GCGCAGTGAG TCAGACTTCC CCCCAGTCTT CCACATCAAA 
CTCAAGGACC AGGTGCTGCT GGAGGGGGAG GCAGCCACCC TGCTCTGCCT GCCAGCGGCC 
TGCCCTGCAC CGCACATCTC CTGGATGAAA GACAAGAAGT CCTTGAGGTC AGAGCCCTCA 
GTGATCATCG TGTCCTGCAA AGATGGGCGG CAGCTGCTCA GCATCCCCCG GGCGGGCAAG 
CGGCACGCCG GTCTCTATGA GTGCTCGGCC ACCAACGTAC TGGGCAGCAT CACCAGCTCC 
TGTACCGTGG CTGTGGCCCG AGTCCCAGGA AAGCTAGCTC CTCCAGAGGT ACCCCAGACC 
TACCAGGACA CGGCGCTGGT GCTGTGGAAG CCGGGAGACA GCCGGGCACC TTGCACGTAT 
ACGCTGGAGC GGCGAGTGGA TGGGGAGTCT GTGTGGCACC CTGTGAGCTC AGGCATCCCC 
GACTGTTACT ACAACGTGAC CCACCTGCCA GTTGGCGTGA CTGTGAGGTT CCGTGTGGCC 
TGTGCCAACC GTGCTGGGCA GGGGCCCTTC AGCAACTCTT CTGAGAAGGT CTTTGTCAGG 
GGTACTCAAG ATTCTTCAGC TGTGCCATCT GCTGCCCACC AAGAGGCCCC TGTCACCTCA 
AGGCCAGCCA GGGCCCGGCC TCCTGACTCT CCTACCTCAC TGGCCCCACC CCTAGCTCCT 
GCTGCCCCCA CACCCCCGTC AGTCACTGTC AGCCCCTCAT CTCCCCCCAC ACCTCCTAGC 
CAGGCCTTGT CCTCGCTCAA GGCTGTGGGT CCACCACCCC AAACCCCTCC ACGAAGACAC 
AGGGGCCTGC AGGCTGCCCG GCCAGCGGAG CCCACCCTAC CCAGTACCCA CGTCACCCCA 
AGTGAGCCCA AGCCTTTCGT CCTTGACACT GGGACCCCGA TCCCAGCCTC CACTCCTCAA 
GGGGTTAAAC CAGTGTCTTC CTCTACTCCT GTGTATGTGG TGACTTCCTT TGTGTCTGCA 
CCACCAGCCC CTGAGCCCCC AGCCCCTGAG CCCCCTCCTG AGCCTACCAA GGTGACTGTG 
CAGAGCCTCA GCCCGGCCAA GGAGGTGGTC AGCTCCCCTG GGAGCAGTCC CCGAAGCTCT 
CCCAGGCCTG AGGGTACCAC TCTTCGACAG GGTCCCCCTC AGAAACCCTA CACCTTCCTG 
GAGGAGAAAG CCAGGGGCCG CTTTGGTGTT GTGCGAGCGT GCCGGGAGAA TGCCACGGGG 
CGAACGTTCG TGGCCAAGAT CGTGCCCTAT GCTGCCGAGG GCAAGCGGCG GGTCCTGCAG 
GAGTACGAGG TGCTGCGGAC CCTGCACCAC GAGCGGATCA TGTCCCTGCA CGAGGCCTAC 
ATCACCCCTC GGTACCTCGT GCTCATTGCT GAGAGCTGTG GCAACCGGGA ACTCCTCTGT 
GGGCTCAGTG ACAGGTTCCG GTATTCTGAG GATGACGTGG CCACTTACAT GGTGCAGCTG 
CTACAAGGCC TGGACTACCT CCACGGCCAC CACGTGCTCC ACCTAGACAT CAAGCCAGAC 
AACCTGCTGC TGGCCCCTGA CAATGCCCTC AAGATTGTGG ACTTTGGCAG TGCCCAGCCC 
TACAACCCCC AGGCCCTTAG GCCCCTTGGC CACCGCACGG GCACGCTGGA GTTCATGGCT 
CCGGAGATGG TGAAGGGAGA ACCCATCGGC TCTGCCACGG ACATCTGGGG AGCGGGTGTG 
CTCACTTACA TTATGCTCAG TGGACGCTCC CCGTTCTATG AGCCAGACCC CCAGGAAACG 
GAGGCTCGGA TTGTGGGGGG CCGCTTTGAT GCCTTCCAGC TGTACCCCAA TACATCCCAG 
AGCGCCACCC TCTTCTTGCG AAAGGTTCTC TCTGTACATC CCTGGAGCCG GCCCTCCCTG 
CAGGACTGCC TGGCCCACCC ATGGTTGCAG GACGCCTACC TGATGAAGCT GCGCCGCCAG 
ACGCTCACCT TCACCACCAA CCGGCTCAAG GAGTTCCTGG GCGAGCAGCG GCGGCGCCGG 
GCTGAGGCTG CCACCCGCCA CAAGGTGCTG CTGCGCTCCT ACCCTGGCGG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_005246240.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005246297.1 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X10, mRNA.

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CloneID	OHu35758
Clone ID Related Accession (Same CDS sequence)	XM_017003158.1 , XM_005246241.1 , XM_006712193.3 , XM_017003158.2 , XM_005246240.2
Accession Version	XM_005246240.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced XM_005246240.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCCA GTCCCAGCCA GAACCGCCGT TCTTCTGACA CTGGCTCCAA GGCACCCCCC 
ACCTTCAAGG TCTCACTTAT GGACCAGTCA GTAAGAGAAG GCCAAGATGT CATCATGAGC 
ATCCGCGTGC AGGGGGAGCC CAAGCCTGTG GTCTCCTGGC TGAGAAACCG CCAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCGGAGGAG GCTGAGGGTG GGCTGTGCCG GCTGCGGATC 
CTGGCTGCAG AGCGTGGCGA TGCTGGTTTC TACACTTGCA AAGCGGTCAA TGAGTATGGT 
GCTCGGCAGT GCGAGGCCCG CTTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCGG GGCCCACTGA CGTGGAGGTG GATTGGCTGT GCCGTGGCCG CCTGCTGCAG 
CCTGCACTGC TCAAATGCAA GATGCATTTC GATGGCCGCA AATGCAAGCT GCTACTTACA 
TCTGTACATG AGGACGACAG TGGCGTCTAC ACCTGCAAGC TCAGCACGGC CAAAGATGAG 
CTGACCTGCA GTGCCCGGCT GACCGTGCGG CCCTCGTTGG CACCCCTGTT CACACGGCTG 
CTGGAAGATG TGGAGGTGTT GGAGGGCCGA GCTGCCCGTT TCGACTGCAA GATCAGTGGC 
ACCCCGCCCC CTGTTGTTAC CTGGACTCAT TTTGGCTGCC CCATGGAGGA GAGTGAGAAC 
TTGCGGCTGC GGCAGGACGG GGGTCTGCAC TCACTGCACA TTGCCCATGT GGGCAGCGAG 
GACGAGGGGC TCTATGCGGT CAGTGCTGTT AACACCCATG GCCAGGCCCA CTGCTCAGCC 
CAGCTGTATG TAGAAGAGCC CCGGACAGCC GCCTCAGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCCA TTCCCGAGGA GCCAGAGCAG GGTGAGCTGG AGCGGCTGTC CATTCCTGAC 
TTCCTGCGGC CACTGCAGGA CCTGGAGGTG GGACTGGCCA AGGAGGCCAT GCTAGAGTGC 
CAGGTGACCG GCCTGCCCTA CCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATCCAG 
AGCAGCGACG ACCGGCGCAT GACACAGTAC AGGGATGTCC ATCGCTTGGT GTTCCCTGCC 
GTGGGGCCTC AGCACGCCGG TGTCTACAAG AGCGTCATTG CCAACAAGCT GGGCAAAGCT 
GCCTGCTATG CCCACCTGTA TGTCACAGAT GTGGTCCCAG GCCCTCCAGA TGGCGCCCCG 
CAGGTGGTGG CTGTGACGGG GAGGATGGTC ACACTCACAT GGAACCCCCC CAGGAGTCTG 
GACATGGCCA TCGACCCGGA CTCCCTGACG TACACAGTGC AGCACCAGGT GCTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCAG GGTGGGCAGC CACAGGGCTG 
CGTAAGGGGG TCCAGCACAT CTTCCGGGTC CTCAGCACCA CTGTCAAGAG CAGCAGCAAG 
CCCTCACCCC CTTCTGAGCC TGTGCAGCTG CTGGAGCACG GCCCAACCCT GGAGGAGGCC 
CCTGCCATGC TGGACAAACC AGACATCGTG TATGTGGTGG AGGGACAGCC TGCCAGCGTC 
ACCGTCACAT TCAACCATGT GGAGGCCCAG GTCGTCTGGA GGAGCTGCCG AGGGGCCCTC 
CTAGAGGCAC GGGCCGGTGT GTACGAGCTG AGCCAGCCAG ATGATGACCA GTACTGTCTT 
CGGATCTGCC GGGTGAGCCG CCGGGACATG GGGGCCCTCA CCTGCACCGC CCGAAACCGT 
CACGGCACAC AGACCTGCTC GGTCACATTG GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC 
ATCATGGAGG ACGTGGAGGT GGGGGCTGGG GAAACTGCTC GCTTTGCGGT GGTGGTCGAG 
GGAAAACCAC TGCCGGACAT CATGTGGTAC AAGGACGAGG TGCTGCTGAC CGAGAGCAGC 
CATGTGAGCT TCGTGTACGA GGAGAATGAG TGCTCCCTGG TGGTGCTCAG CACGGGGGCC 
CAGGATGGAG GCGTCTACAC CTGCACCGCC CAGAACCTGG CGGGTGAGGT CTCCTGCAAA 
GCAGAGTTGG CTGTGCATTC AGCTCAGACA GCTATGGAGG TCGAGGGGGT CGGGGAGGAT 
GAGGACCATC GAGGAAGGAG ACTCAGCGAC TTTTATGACA TCCACCAGGA GATCGGCAGG 
GGTGCTTTCT CCTACTTGCG GCGCATAGTG GAGCGTAGCT CCGGCCTGGA GTTTGCGGCC 
AAGTTCATCC CCAGCCAGGC CAAGCCAAAG GCATCAGCGC GTCGGGAGGC CCGGCTGCTG 
GCCAGGCTCC AGCACGACTG TGTCCTCTAC TTCCATGAGG CCTTCGAGAG GCGCCGGGGA 
CTGGTCATTG TCACCGAGCT CTGCACAGAG GAGCTGCTGG AGCGAATCGC CAGGAAACCC 
ACCGTGTGTG AGTCTGAGAT CCGGGCCTAT ATGCGGCAGG TGCTAGAGGG AATACACTAC 
CTGCACCAGA GCCACGTGCT GCACCTCGAT GTCAAGCCTG AGAACCTGCT GGTGTGGGAT 
GGTGCTGCGG GCGAGCAGCA GGTGCGGATC TGTGACTTTG GGAATGCCCA GGAGCTGACT 
CCAGGAGAGC CCCAGTACTG CCAGTATGGC ACACCTGAGT TTGTAGCACC CGAGATTGTC 
AATCAGAGCC CCGTGTCTGG AGTCACTGAC ATCTGGCCTG TGGGTGTTGT TGCCTTCCTC 
TGTCTGACAG GAATCTCCCC GTTTGTTGGG GAAAATGACC GGACAACATT GATGAACATC 
CGAAACTACA ACGTGGCCTT CGAGGAGACC ACATTCCTGA GCCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGTT GGTGCAGGAC CGGCTGAGAC CTACCGCAGA AGAGACCCTA 
GAACATCCTT GGTTCAAAAC TCAGGCAAAG GGCGCAGAGG TGAGCACGGA TCACCTGAAG 
CTATTCCTCT CCCGGCGGAG GTGGCAGCGC TCCCAGATCA GCTACAAATG CCACCTGGTG 
CTGCGCCCCA TCCCCGAGCT GCTGCGGGCC CCCCCAGAGC GGGTGTGGGT GACCATGCCC 
AGAAGGCCAC CCCCCAGTGG GGGGCTCTCA TCCTCCTCGG ATTCTGAAGA GGAAGAGCTG 
GAAGAGCTGC CCTCAGTGCC CCGCCCACTG CAGCCCGAGT TCTCTGGCTC CCGGGTGTCC 
CTCACAGACA TTCCCACTGA GGATGAGGCC CTGGGGACCC CAGAGACTGG GGCTGCCACC 
CCCATGGACT GGCAGGAGCA GGGAAGGGCT CCCTCTCAGG ACCAGGAGGC TCCCAGCCCA 
GAGGCCCTCC CCTCCCCAGG CCAGGAGCCC GCAGCTGGGG CTAGCCCCAG GCGGGGAGAG 
CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC CTGCCCCGGG CCGGGCCGCG GGAGCTGGGC 
CGGGGCCTGC ACAAGGCGGC GTCTGTGGAG CTGCCGCAGC GCCGGAGCCC CAGCCCGGGA 
GCCACCCGCC TGGCCCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGCCAGC GGCTGCTGCG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGT 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCT CGGGACCCCC GGATGGCACG AGCTGCCTCC 
AGCGAGGCAG CGCCCCACCA CCAGCCCCCA CTCGAGAACC GGGGCCTGCA AAAGAGCAGC 
AGCTTCTCCC AGGGTGAGGC GGAGCCCCGG GGCCGGCACC GCCGAGCGGG GGCGCCCCTC 
GAGATCCCCG TGGCCAGGCT TGGGGCCCGT AGGCTACAGG AGTCTCCTTC CCTGTCTGCC 
CTCAGCGAGG CCCAGCCATC CAGCCCTGCA CGGCCCAGCG CCCCCAAACC CAGTACCCCT 
AAGTCTGCAG AACCTTCTGC CACCACACCT AGTGATGCTC CGCAGCCCCC CGCACCCCAG 
CCTGCCCAAG ACAAGGCTCC AGAGCCCAGG CCAGAACCAG TCCGAGCCTC CAAGCCTGCA 
CCACCCCCCC AGGCCCTGCA AACCCTAGCG CTGCCCCTCA CACCCTATGC TCAGATCATT 
CAGTCCCTCC AGCTGTCAGG CCACGCCCAG GGCCCCTCGC AGGGCCCTGC CGCGCCGCCT 
TCAGAGCCCA AGCCCCACGC TGCTGTCTTT GCCAGGGTGG CCTCCCCACC TCCGGGAGCC 
CCCGAGAAGC GCGTGCCCTC AGCCGGGGGT CCCCCGGTGC TAGCCGAGAA AGCCCGAGTT 
CCCACGGTGC CCCCCAGGCC AGGCAGCAGT CTCAGTAGCA GCATCGAAAA CTTGGAGTCG 
GAGGCCGTGT TCGAGGCCAA GTTCAAGCGC AGCCGCGAGT CGCCCCTGTC GCTGGGGCTG 
CGGCTGCTGA GCCGTTCGCG CTCGGAGGAG CGCGGCCCCT TCCGTGGGGC CGAGGAGGAG 
GATGGCATAT ACCGGCCCAG CCCGGCGGGG ACCCCGCTGG AGCTGGTGCG ACGGCCTGAG 
CGCTCACGCT CGGTGCAGGA CCTCAGGGCT GTCGGAGAGC CTGGCCTCGT CCGCCGCCTC 
TCGCTGTCAC TGTCCCAGCG GCTGCGGCGG ACCCCTCCCG CGCAGCGCCA CCCGGCCTGG 
GAGGCCCGCG GCGGGGACGG AGAGAGCTCG GAGGGCGGGA GCTCGGCGCG GGGCTCCCCG 
GTGCTGGCGA TGCGCAGGCG GCTGAGCTTC ACCCTGGAGC GGCTGTCCAG CCGATTGCAG 
CGCAGTGGCA GCAGCGAGGA CTCGGGGGGC GCGTCGGGCC GCAGCACGCC GCTGTTCGGA 
CGGCTTCGCA GGGCCACGTC CGAGGGCGAG AGTCTGCGGC GCCTTGGCCT TCCGCACAAC 
CAGTTGGCCG CCCAGGCCGG CGCCACCACG CCTTCCGCCG AGTCCCTGGG CTCCGAGGCC 
AGCGCCACGT CGGGCTCCTC AGCCCCAGGG GAAAGCCGAA GCCGGCTCCG CTGGGGCTTC 
TCTCGGCCGC GGAAGGACAA GGGGTTATCG CCACCAAACC TCTCTGCCAG CGTCCAGGAG 
GAGTTGGGTC ACCAGTACGT GCGCAGTGAG TCAGACTTCC CCCCAGTCTT CCACATCAAA 
CTCAAGGACC AGGTGCTGCT GGAGGGGGAG GCAGCCACCC TGCTCTGCCT GCCAGCGGCC 
TGCCCTGCAC CGCACATCTC CTGGATGAAA GACAAGAAGT CCTTGAGGTC AGAGCCCTCA 
GTGATCATCG TGTCCTGCAA AGATGGGCGG CAGCTGCTCA GCATCCCCCG GGCGGGCAAG 
CGGCACGCCG GTCTCTATGA GTGCTCGGCC ACCAACGTAC TGGGCAGCAT CACCAGCTCC 
TGTACCGTGG CTGTGGCCCG AGTCCCAGGA AAGCTAGCTC CTCCAGAGGT ACCCCAGACC 
TACCAGGACA CGGCGCTGGT GCTGTGGAAG CCGGGAGACA GCCGGGCACC TTGCACGTAT 
ACGCTGGAGC GGCGAGTGGA TGGGGAGTCT GTGTGGCACC CTGTGAGCTC AGGCATCCCC 
GACTGTTACT ACAACGTGAC CCACCTGCCA GTTGGCGTGA CTGTGAGGTT CCGTGTGGCC 
TGTGCCAACC GTGCTGGGCA GGGGCCCTTC AGCAACTCTT CTGAGAAGGT CTTTGTCAGG 
GGTACTCAAG ATTCTTCAGC TGTGCCATCT GCTGCCCACC AAGAGGCCCC TGTCACCTCA 
AGGCCAGCCA GGGCCCGGCC TCCTGACTCT CCTACCTCAC TGGCCCCACC CCTAGCTCCT 
GCTGCCCCCA CACCCCCGTC AGTCACTGTC AGCCCCTCAT CTCCCCCCAC ACCTCCTAGC 
CAGGCCTTGT CCTCGCTCAA GGCTGTGGGT CCACCACCCC AAACCCCTCC ACGAAGACAC 
AGGGGCCTGC AGGCTGCCCG GCCAGCGGAG CCCACCCTAC CCAGTACCCA CGTCACCCCA 
AGTGAGCCCA AGCCTTTCGT CCTTGACACT GGGACCCCGA TCCCAGCCTC CACTCCTCAA 
GGGGTTAAAC CAGTGTCTTC CTCTACTCCT GTGTATGTGG TGACTTCCTT TGTGTCTGCA 
CCACCAGCCC CTGAGCCCCC AGCCCCTGAG CCCCCTCCTG AGCCTACCAA GGTGACTGTG 
CAGAGCCTCA GCCCGGCCAA GGAGGTGGTC AGCTCCCCTG GGAGCAGTCC CCGAAGCTCT 
CCCAGGCCTG AGGGTACCAC TCTTCGACAG GGTCCCCCTC AGAAACCCTA CACCTTCCTG 
GAGGAGAAAG CCAGGGGCCG CTTTGGTGTT GTGCGAGCGT GCCGGGAGAA TGCCACGGGG 
CGAACGTTCG TGGCCAAGAT CGTGCCCTAT GCTGCCGAGG GCAAGCGGCG GGTCCTGCAG 
GAGTACGAGG TGCTGCGGAC CCTGCACCAC GAGCGGATCA TGTCCCTGCA CGAGGCCTAC 
ATCACCCCTC GGTACCTCGT GCTCATTGCT GAGAGCTGTG GCAACCGGGA ACTCCTCTGT 
GGGCTCAGTG ACAGGTTCCG GTATTCTGAG GATGACGTGG CCACTTACAT GGTGCAGCTG 
CTACAAGGCC TGGACTACCT CCACGGCCAC CACGTGCTCC ACCTAGACAT CAAGCCAGAC 
AACCTGCTGC TGGCCCCTGA CAATGCCCTC AAGATTGTGG ACTTTGGCAG TGCCCAGCCC 
TACAACCCCC AGGCCCTTAG GCCCCTTGGC CACCGCACGG GCACGCTGGA GTTCATGGCT 
CCGGAGATGG TGAAGGGAGA ACCCATCGGC TCTGCCACGG ACATCTGGGG AGCGGGTGTG 
CTCACTTACA TTATGCTCAG TGGACGCTCC CCGTTCTATG AGCCAGACCC CCAGGAAACG 
GAGGCTCGGA TTGTGGGGGG CCGCTTTGAT GCCTTCCAGC TGTACCCCAA TACATCCCAG 
AGCGCCACCC TCTTCTTGCG AAAGGTTCTC TCTGTACATC CCTGGAGCCG GCCCTCCCTG 
CAGGACTGCC TGGCCCACCC ATGGTTGCAG GACGCCTACC TGATGAAGCT GCGCCGCCAG 
ACGCTCACCT TCACCACCAA CCGGCTCAAG GAGTTCCTGG GCGAGCAGCG GCGGCGCCGG 
GCTGAGGCTG CCACCCGCCA CAAGGTGCTG CTGCGCTCCT ACCCTGGCGG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005246297.1
CDS	293..7549
Translation	MKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPTDVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARFDCKISGTPPPVVTWTHFGCPMEESENLRLRQDGGLHSLHIAHVGSEDEGLYAVSAVNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEQGELERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDVHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPQVVAVTGRMVTLTWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPGWAATGLRKGVQHIFRVLSTTVKSSSKPSPPSEPVQLLEHGPTLEEAPAMLDKPDIVYVVEGQPASVTVTFNHVEAQVVWRSCRGALLEARAGVYELSQPDDDQYCLRICRVSRRDMGALTCTARNRHGTQTCSVTLELAEAPRFESIMEDVEVGAGETARFAVVVEGKPLPDIMWYKDEVLLTESSHVSFVYEENECSLVVLSTGAQDGGVYTCTAQNLAGEVSCKAELAVHSAQTAMEVEGVGEDEDHRGRRLSDFYDIHQEIGRGAFSYLRRIVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHDCVLYFHEAFERRRGLVIVTELCTEELLERIARKPTVCESEIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTQAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGELRRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQPPAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPSEPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEAKFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAVGEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAESLGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPHISWMKDKKSLRSEPSVIIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARVPGKLAPPEVPQTYQDTALVLWKPGDSRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTHLPVGVTVRFRVACANRAGQGPFSNSSEKVFVRGTQDSSAVPSAAHQEAPVTSRPARARPPDSPTSLAPPLAPAAPTPPSVTVSPSSPPTPPSQALSSLKAVGPPPQTPPRRHRGLQAARPAEPTLPSTHVTPSEPKPFVLDTGTPIPASTPQGVKPVSSSTPVYVVTSFVSAPPAPEPPAPEPPPEPTKVTVQSLSPAKEVVSSPGSSPRSSPRPEGTTLRQGPPQKPYTFLEEKARGRFGVVRACRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERIMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYMVQLLQGLDYLHGHHVLHLDIKPDNLLLAPDNALKIVDFGSAQPYNPQALRPLGHRTGTLEFMAPEMVKGEPIGSATDIWGAGVLTYIMLSGRSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGGP

Target ORF information:

RefSeq Version	XM_005246240.2
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246240.2

ORF Insert Sequence:

ATGAAGCCCA GTCCCAGCCA GAACCGCCGT TCTTCTGACA CTGGCTCCAA GGCACCCCCC 
ACCTTCAAGG TCTCACTTAT GGACCAGTCA GTAAGAGAAG GCCAAGATGT CATCATGAGC 
ATCCGCGTGC AGGGGGAGCC CAAGCCTGTG GTCTCCTGGC TGAGAAACCG CCAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCGGAGGAG GCTGAGGGTG GGCTGTGCCG GCTGCGGATC 
CTGGCTGCAG AGCGTGGCGA TGCTGGTTTC TACACTTGCA AAGCGGTCAA TGAGTATGGT 
GCTCGGCAGT GCGAGGCCCG CTTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCGG GGCCCACTGA CGTGGAGGTG GATTGGCTGT GCCGTGGCCG CCTGCTGCAG 
CCTGCACTGC TCAAATGCAA GATGCATTTC GATGGCCGCA AATGCAAGCT GCTACTTACA 
TCTGTACATG AGGACGACAG TGGCGTCTAC ACCTGCAAGC TCAGCACGGC CAAAGATGAG 
CTGACCTGCA GTGCCCGGCT GACCGTGCGG CCCTCGTTGG CACCCCTGTT CACACGGCTG 
CTGGAAGATG TGGAGGTGTT GGAGGGCCGA GCTGCCCGTT TCGACTGCAA GATCAGTGGC 
ACCCCGCCCC CTGTTGTTAC CTGGACTCAT TTTGGCTGCC CCATGGAGGA GAGTGAGAAC 
TTGCGGCTGC GGCAGGACGG GGGTCTGCAC TCACTGCACA TTGCCCATGT GGGCAGCGAG 
GACGAGGGGC TCTATGCGGT CAGTGCTGTT AACACCCATG GCCAGGCCCA CTGCTCAGCC 
CAGCTGTATG TAGAAGAGCC CCGGACAGCC GCCTCAGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCCA TTCCCGAGGA GCCAGAGCAG GGTGAGCTGG AGCGGCTGTC CATTCCTGAC 
TTCCTGCGGC CACTGCAGGA CCTGGAGGTG GGACTGGCCA AGGAGGCCAT GCTAGAGTGC 
CAGGTGACCG GCCTGCCCTA CCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATCCAG 
AGCAGCGACG ACCGGCGCAT GACACAGTAC AGGGATGTCC ATCGCTTGGT GTTCCCTGCC 
GTGGGGCCTC AGCACGCCGG TGTCTACAAG AGCGTCATTG CCAACAAGCT GGGCAAAGCT 
GCCTGCTATG CCCACCTGTA TGTCACAGAT GTGGTCCCAG GCCCTCCAGA TGGCGCCCCG 
CAGGTGGTGG CTGTGACGGG GAGGATGGTC ACACTCACAT GGAACCCCCC CAGGAGTCTG 
GACATGGCCA TCGACCCGGA CTCCCTGACG TACACAGTGC AGCACCAGGT GCTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCAG GGTGGGCAGC CACAGGGCTG 
CGTAAGGGGG TCCAGCACAT CTTCCGGGTC CTCAGCACCA CTGTCAAGAG CAGCAGCAAG 
CCCTCACCCC CTTCTGAGCC TGTGCAGCTG CTGGAGCACG GCCCAACCCT GGAGGAGGCC 
CCTGCCATGC TGGACAAACC AGACATCGTG TATGTGGTGG AGGGACAGCC TGCCAGCGTC 
ACCGTCACAT TCAACCATGT GGAGGCCCAG GTCGTCTGGA GGAGCTGCCG AGGGGCCCTC 
CTAGAGGCAC GGGCCGGTGT GTACGAGCTG AGCCAGCCAG ATGATGACCA GTACTGTCTT 
CGGATCTGCC GGGTGAGCCG CCGGGACATG GGGGCCCTCA CCTGCACCGC CCGAAACCGT 
CACGGCACAC AGACCTGCTC GGTCACATTG GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC 
ATCATGGAGG ACGTGGAGGT GGGGGCTGGG GAAACTGCTC GCTTTGCGGT GGTGGTCGAG 
GGAAAACCAC TGCCGGACAT CATGTGGTAC AAGGACGAGG TGCTGCTGAC CGAGAGCAGC 
CATGTGAGCT TCGTGTACGA GGAGAATGAG TGCTCCCTGG TGGTGCTCAG CACGGGGGCC 
CAGGATGGAG GCGTCTACAC CTGCACCGCC CAGAACCTGG CGGGTGAGGT CTCCTGCAAA 
GCAGAGTTGG CTGTGCATTC AGCTCAGACA GCTATGGAGG TCGAGGGGGT CGGGGAGGAT 
GAGGACCATC GAGGAAGGAG ACTCAGCGAC TTTTATGACA TCCACCAGGA GATCGGCAGG 
GGTGCTTTCT CCTACTTGCG GCGCATAGTG GAGCGTAGCT CCGGCCTGGA GTTTGCGGCC 
AAGTTCATCC CCAGCCAGGC CAAGCCAAAG GCATCAGCGC GTCGGGAGGC CCGGCTGCTG 
GCCAGGCTCC AGCACGACTG TGTCCTCTAC TTCCATGAGG CCTTCGAGAG GCGCCGGGGA 
CTGGTCATTG TCACCGAGCT CTGCACAGAG GAGCTGCTGG AGCGAATCGC CAGGAAACCC 
ACCGTGTGTG AGTCTGAGAT CCGGGCCTAT ATGCGGCAGG TGCTAGAGGG AATACACTAC 
CTGCACCAGA GCCACGTGCT GCACCTCGAT GTCAAGCCTG AGAACCTGCT GGTGTGGGAT 
GGTGCTGCGG GCGAGCAGCA GGTGCGGATC TGTGACTTTG GGAATGCCCA GGAGCTGACT 
CCAGGAGAGC CCCAGTACTG CCAGTATGGC ACACCTGAGT TTGTAGCACC CGAGATTGTC 
AATCAGAGCC CCGTGTCTGG AGTCACTGAC ATCTGGCCTG TGGGTGTTGT TGCCTTCCTC 
TGTCTGACAG GAATCTCCCC GTTTGTTGGG GAAAATGACC GGACAACATT GATGAACATC 
CGAAACTACA ACGTGGCCTT CGAGGAGACC ACATTCCTGA GCCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGTT GGTGCAGGAC CGGCTGAGAC CTACCGCAGA AGAGACCCTA 
GAACATCCTT GGTTCAAAAC TCAGGCAAAG GGCGCAGAGG TGAGCACGGA TCACCTGAAG 
CTATTCCTCT CCCGGCGGAG GTGGCAGCGC TCCCAGATCA GCTACAAATG CCACCTGGTG 
CTGCGCCCCA TCCCCGAGCT GCTGCGGGCC CCCCCAGAGC GGGTGTGGGT GACCATGCCC 
AGAAGGCCAC CCCCCAGTGG GGGGCTCTCA TCCTCCTCGG ATTCTGAAGA GGAAGAGCTG 
GAAGAGCTGC CCTCAGTGCC CCGCCCACTG CAGCCCGAGT TCTCTGGCTC CCGGGTGTCC 
CTCACAGACA TTCCCACTGA GGATGAGGCC CTGGGGACCC CAGAGACTGG GGCTGCCACC 
CCCATGGACT GGCAGGAGCA GGGAAGGGCT CCCTCTCAGG ACCAGGAGGC TCCCAGCCCA 
GAGGCCCTCC CCTCCCCAGG CCAGGAGCCC GCAGCTGGGG CTAGCCCCAG GCGGGGAGAG 
CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC CTGCCCCGGG CCGGGCCGCG GGAGCTGGGC 
CGGGGCCTGC ACAAGGCGGC GTCTGTGGAG CTGCCGCAGC GCCGGAGCCC CAGCCCGGGA 
GCCACCCGCC TGGCCCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGCCAGC GGCTGCTGCG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGT 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCT CGGGACCCCC GGATGGCACG AGCTGCCTCC 
AGCGAGGCAG CGCCCCACCA CCAGCCCCCA CTCGAGAACC GGGGCCTGCA AAAGAGCAGC 
AGCTTCTCCC AGGGTGAGGC GGAGCCCCGG GGCCGGCACC GCCGAGCGGG GGCGCCCCTC 
GAGATCCCCG TGGCCAGGCT TGGGGCCCGT AGGCTACAGG AGTCTCCTTC CCTGTCTGCC 
CTCAGCGAGG CCCAGCCATC CAGCCCTGCA CGGCCCAGCG CCCCCAAACC CAGTACCCCT 
AAGTCTGCAG AACCTTCTGC CACCACACCT AGTGATGCTC CGCAGCCCCC CGCACCCCAG 
CCTGCCCAAG ACAAGGCTCC AGAGCCCAGG CCAGAACCAG TCCGAGCCTC CAAGCCTGCA 
CCACCCCCCC AGGCCCTGCA AACCCTAGCG CTGCCCCTCA CACCCTATGC TCAGATCATT 
CAGTCCCTCC AGCTGTCAGG CCACGCCCAG GGCCCCTCGC AGGGCCCTGC CGCGCCGCCT 
TCAGAGCCCA AGCCCCACGC TGCTGTCTTT GCCAGGGTGG CCTCCCCACC TCCGGGAGCC 
CCCGAGAAGC GCGTGCCCTC AGCCGGGGGT CCCCCGGTGC TAGCCGAGAA AGCCCGAGTT 
CCCACGGTGC CCCCCAGGCC AGGCAGCAGT CTCAGTAGCA GCATCGAAAA CTTGGAGTCG 
GAGGCCGTGT TCGAGGCCAA GTTCAAGCGC AGCCGCGAGT CGCCCCTGTC GCTGGGGCTG 
CGGCTGCTGA GCCGTTCGCG CTCGGAGGAG CGCGGCCCCT TCCGTGGGGC CGAGGAGGAG 
GATGGCATAT ACCGGCCCAG CCCGGCGGGG ACCCCGCTGG AGCTGGTGCG ACGGCCTGAG 
CGCTCACGCT CGGTGCAGGA CCTCAGGGCT GTCGGAGAGC CTGGCCTCGT CCGCCGCCTC 
TCGCTGTCAC TGTCCCAGCG GCTGCGGCGG ACCCCTCCCG CGCAGCGCCA CCCGGCCTGG 
GAGGCCCGCG GCGGGGACGG AGAGAGCTCG GAGGGCGGGA GCTCGGCGCG GGGCTCCCCG 
GTGCTGGCGA TGCGCAGGCG GCTGAGCTTC ACCCTGGAGC GGCTGTCCAG CCGATTGCAG 
CGCAGTGGCA GCAGCGAGGA CTCGGGGGGC GCGTCGGGCC GCAGCACGCC GCTGTTCGGA 
CGGCTTCGCA GGGCCACGTC CGAGGGCGAG AGTCTGCGGC GCCTTGGCCT TCCGCACAAC 
CAGTTGGCCG CCCAGGCCGG CGCCACCACG CCTTCCGCCG AGTCCCTGGG CTCCGAGGCC 
AGCGCCACGT CGGGCTCCTC AGCCCCAGGG GAAAGCCGAA GCCGGCTCCG CTGGGGCTTC 
TCTCGGCCGC GGAAGGACAA GGGGTTATCG CCACCAAACC TCTCTGCCAG CGTCCAGGAG 
GAGTTGGGTC ACCAGTACGT GCGCAGTGAG TCAGACTTCC CCCCAGTCTT CCACATCAAA 
CTCAAGGACC AGGTGCTGCT GGAGGGGGAG GCAGCCACCC TGCTCTGCCT GCCAGCGGCC 
TGCCCTGCAC CGCACATCTC CTGGATGAAA GACAAGAAGT CCTTGAGGTC AGAGCCCTCA 
GTGATCATCG TGTCCTGCAA AGATGGGCGG CAGCTGCTCA GCATCCCCCG GGCGGGCAAG 
CGGCACGCCG GTCTCTATGA GTGCTCGGCC ACCAACGTAC TGGGCAGCAT CACCAGCTCC 
TGTACCGTGG CTGTGGCCCG AGTCCCAGGA AAGCTAGCTC CTCCAGAGGT ACCCCAGACC 
TACCAGGACA CGGCGCTGGT GCTGTGGAAG CCGGGAGACA GCCGGGCACC TTGCACGTAT 
ACGCTGGAGC GGCGAGTGGA TGGGGAGTCT GTGTGGCACC CTGTGAGCTC AGGCATCCCC 
GACTGTTACT ACAACGTGAC CCACCTGCCA GTTGGCGTGA CTGTGAGGTT CCGTGTGGCC 
TGTGCCAACC GTGCTGGGCA GGGGCCCTTC AGCAACTCTT CTGAGAAGGT CTTTGTCAGG 
GGTACTCAAG ATTCTTCAGC TGTGCCATCT GCTGCCCACC AAGAGGCCCC TGTCACCTCA 
AGGCCAGCCA GGGCCCGGCC TCCTGACTCT CCTACCTCAC TGGCCCCACC CCTAGCTCCT 
GCTGCCCCCA CACCCCCGTC AGTCACTGTC AGCCCCTCAT CTCCCCCCAC ACCTCCTAGC 
CAGGCCTTGT CCTCGCTCAA GGCTGTGGGT CCACCACCCC AAACCCCTCC ACGAAGACAC 
AGGGGCCTGC AGGCTGCCCG GCCAGCGGAG CCCACCCTAC CCAGTACCCA CGTCACCCCA 
AGTGAGCCCA AGCCTTTCGT CCTTGACACT GGGACCCCGA TCCCAGCCTC CACTCCTCAA 
GGGGTTAAAC CAGTGTCTTC CTCTACTCCT GTGTATGTGG TGACTTCCTT TGTGTCTGCA 
CCACCAGCCC CTGAGCCCCC AGCCCCTGAG CCCCCTCCTG AGCCTACCAA GGTGACTGTG 
CAGAGCCTCA GCCCGGCCAA GGAGGTGGTC AGCTCCCCTG GGAGCAGTCC CCGAAGCTCT 
CCCAGGCCTG AGGGTACCAC TCTTCGACAG GGTCCCCCTC AGAAACCCTA CACCTTCCTG 
GAGGAGAAAG CCAGGGGCCG CTTTGGTGTT GTGCGAGCGT GCCGGGAGAA TGCCACGGGG 
CGAACGTTCG TGGCCAAGAT CGTGCCCTAT GCTGCCGAGG GCAAGCGGCG GGTCCTGCAG 
GAGTACGAGG TGCTGCGGAC CCTGCACCAC GAGCGGATCA TGTCCCTGCA CGAGGCCTAC 
ATCACCCCTC GGTACCTCGT GCTCATTGCT GAGAGCTGTG GCAACCGGGA ACTCCTCTGT 
GGGCTCAGTG ACAGGTTCCG GTATTCTGAG GATGACGTGG CCACTTACAT GGTGCAGCTG 
CTACAAGGCC TGGACTACCT CCACGGCCAC CACGTGCTCC ACCTAGACAT CAAGCCAGAC 
AACCTGCTGC TGGCCCCTGA CAATGCCCTC AAGATTGTGG ACTTTGGCAG TGCCCAGCCC 
TACAACCCCC AGGCCCTTAG GCCCCTTGGC CACCGCACGG GCACGCTGGA GTTCATGGCT 
CCGGAGATGG TGAAGGGAGA ACCCATCGGC TCTGCCACGG ACATCTGGGG AGCGGGTGTG 
CTCACTTACA TTATGCTCAG TGGACGCTCC CCGTTCTATG AGCCAGACCC CCAGGAAACG 
GAGGCTCGGA TTGTGGGGGG CCGCTTTGAT GCCTTCCAGC TGTACCCCAA TACATCCCAG 
AGCGCCACCC TCTTCTTGCG AAAGGTTCTC TCTGTACATC CCTGGAGCCG GCCCTCCCTG 
CAGGACTGCC TGGCCCACCC ATGGTTGCAG GACGCCTACC TGATGAAGCT GCGCCGCCAG 
ACGCTCACCT TCACCACCAA CCGGCTCAAG GAGTTCCTGG GCGAGCAGCG GCGGCGCCGG 
GCTGAGGCTG CCACCCGCCA CAAGGTGCTG CTGCGCTCCT ACCCTGGCGG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_011510479.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508781.1 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X3, mRNA.

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CloneID	OHu63876
Clone ID Related Accession (Same CDS sequence)	XM_011510479.2
Accession Version	XM_011510479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510479.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GATGCGGGCA CGAGGGCACC CCCCAGCCCC 
GGAGTGCCCC CGAAAAGGGC CAAGGTGGGG GCCGGCGGCG GGGCTCCTGT GGCCGTGGCC 
GGGGCGCCAG TCTTCCTGCG GCCCCTGAAG AACGCGGCGG TGTGCGCGGG CAGCGACGTG 
CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCCGCTGGTT CCGGGATGGG 
CAGCTCCTGC CCGCGCCGGC CCCCGAGCCC AGCTGCCTGT GGCTGCGGCG CTGCGGGGCG 
CAGGACGCCG GCGTGTACAG CTGCATGGCC CAGAACGAGC GGGGCCGGGC CTCCTGCGAG 
GCGGTGCTCA CAGTGCTGGA GGTCGGAGTT TTCCGCTCCT GCAGAAAGCA AAGTTACGAC 
TCAGAGACGG CTGAGGATGA CATCAGCGAT GTGCAGGGAA CCCAGCGCCT GGAGCTTCGG 
GATGACGGGG CCTTCAGCAC CCCCACGGGG GGTTCTGACA CCCTGGTGGG CACCTCCCTG 
GACACACCCC CGACCTCCGT GACAGGCACC TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC 
GGGCAGACGG TCCTGGAGCA GGAAGCGGGC AGTGGGGGTG GCACCCGCCG CCTCCCGGGC 
AGCCCAAGGC AAGCACAGGC AACCGGGGCC GGGCCACGGC ACCTGGGGGT GGAGCCGCTG 
GTGCGGGCAT CTCGAGCTAA TCTGGTGGGC GCAAGCTGGG GGTCAGAGGA TAGCCTTTCC 
GTGGCCAGTG ACCTGTACGG CAGCGCATTC AGCCTGTACA GAGGACGGGC GCTCTCTATC 
CACGTCAGCG TCCCTCAGAG CGGGTTGCGC AGGGAGGAGC CCGACCTTCA GCCTCAACTG 
GCCAGCGAAG CCCCACGCCG CCCTGCCCAG CCGCCTCCTT CCAAATCCGC GCTGCTCCCC 
CCACCGTCCC CTCGGGTCGG GAAGCGGTCC CCGCCGGGAC CCCCGGCCCA GCCCGCGGCC 
ACCCCCACGT CGCCCCACCG TCGCACTCAG GAGCCTGTGC TGCCCGAGGA CACCACCACC 
GAAGAGAAGC GAGGGAAGAA GTCCAAGTCG TCCGGGCCCT CCCTGGCGGG CACCGCGGAA 
TCCCGACCCC AGACGCCACT GAGCGAGGCC TCAGGCCGCC TGTCGGCGTT GGGCCGATCG 
CCTAGGCTGG TGCGCGCCGG CTCCCGCATC CTGGACAAGC TGCAGTTCTT CGAGGAGCGA 
CGGCGCAGCC TGGAGCGCAG CGACTCGCCG CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG 
CGCAAGGCCC GCTCTCTGGA GCAGCCCAAG TCGGAGCGCG GCGCACCGTG GGGCACCCCC 
GGGGCCTCGC AGGAAGAACT GCGGGCGCCA GGCAGCGTGG CCGAGCGGCG CCGCCTGTTC 
CAGCAGAAAG CGGCCTCGCT GGACGAGCGC ACGCGTCAGC GCAGCCCGGC CTCAGACCTC 
GAGCTGCGCT TCGCCCAGGA GCTGGGCCGC ATCCGCCGCT CCACGTCGCG GGAGGAGCTG 
GTGCGCTCGC ACGAGTCCCT GCGCGCCACG CTGCAGCGTG CCCCATCCCC TCGAGAGCCC 
GGCGAGCCCC CGCTCTTCTC TCGGCCCTCC ACCCCCAAGA CATCGCGGGC CGTGAGCCCC 
GCCGCCGCCC AGCCGCCCTC TCCGAGCAGC GCGGAGAAGC CGGGGGACGA GCCTGGGAGG 
CCCAGGAGCC GCGGGCCGGC GGGCAGGACA GAGCCGGGGG AAGGCCCGCA GCAGGAGGTT 
AGGCGTCGGG ACCAATTCCC GCTGACCCGG AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT 
GTGCCGCCCC CCGCCGCCGA TCCCCCAGAG GCCAGGACGA AAGCACCCCC CGGTCGGAAG 
CGGGAGCCCC CGGCGCAGGC CGTGCGCTTC CTGCCCTGGG CCACGCCGGG CCTGGAGGGC 
GCTGCTGTAC CCCAGACCTT GGAGAAGAAC AGGGCGGGGC CTGAGGCAGA GAAGAGGCTT 
CGCAGAGGGC CGGAGGAGGA CGGTCCCTGG GGGCCCTGGG ACCGCCGAGG GGCCCGCAGC 
CAGGGCAAAG GTCGCCGGGC CCGGCCCACC TCCCCTGAGC TCGAGTCTTC GGATGACTCC 
TACGTGTCCG CTGGAGAAGA GCCCCTAGAG GCCCCTGTGT TTGAGATCCC CCTGCAGAAT 
GTGGTGGTGG CACCAGGGGC AGATGTGCTG CTCAAGTGTA TCATCACTGC CAACCCCCCG 
CCCCAAGTGT CCTGGCACAA GGATGGGTCA GCGCTGCGCA GCGAGGGCCG CCTCCTCCTC 
CGGGCTGAGG GTGAGCGGCA CACCCTGCTG CTCAGGGAGG CCAGGGCAGC AGATGCCGGG 
AGCTATATGG CCACCGCCAC CAACGAGCTG GGCCAGGCCA CCTGTGCCGC CTCACTGACC 
GTGAGACCCG GTGGGTCTAC ATCCCCTTTC AGCAGCCCCA TCACCTCCGA CGAGGAATAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCGCGAAC CCCCACCATG 
AAGCCCAGTC CCAGCCAGAA CCGCCGTTCT TCTGACACTG GCTCCAAGGC ACCCCCCACC 
TTCAAGGTCT CACTTATGGA CCAGTCAGTA AGAGAAGGCC AAGATGTCAT CATGAGCATC 
CGCGTGCAGG GGGAGCCCAA GCCTGTGGTC TCCTGGCTGA GAAACCGCCA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC GGAGGAGGCT GAGGGTGGGC TGTGCCGGCT GCGGATCCTG 
GCTGCAGAGC GTGGCGATGC TGGTTTCTAC ACTTGCAAAG CGGTCAATGA GTATGGTGCT 
CGGCAGTGCG AGGCCCGCTT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCGGGGC CCACTGACGT GGAGGTGGAT TGGCTGTGCC GTGGCCGCCT GCTGCAGCCT 
GCACTGCTCA AATGCAAGAT GCATTTCGAT GGCCGCAAAT GCAAGCTGCT ACTTACATCT 
GTACATGAGG ACGACAGTGG CGTCTACACC TGCAAGCTCA GCACGGCCAA AGATGAGCTG 
ACCTGCAGTG CCCGGCTGAC CGTGCGGCCC TCGTTGGCAC CCCTGTTCAC ACGGCTGCTG 
GAAGATGTGG AGGTGTTGGA GGGCCGAGCT GCCCGTTTCG ACTGCAAGAT CAGTGGCACC 
CCGCCCCCTG TTGTTACCTG GACTCATTTT GGCTGCCCCA TGGAGGAGAG TGAGAACTTG 
CGGCTGCGGC AGGACGGGGG TCTGCACTCA CTGCACATTG CCCATGTGGG CAGCGAGGAC 
GAGGGGCTCT ATGCGGTCAG TGCTGTTAAC ACCCATGGCC AGGCCCACTG CTCAGCCCAG 
CTGTATGTAG AAGAGCCCCG GACAGCCGCC TCAGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCCATTC CCGAGGAGCC AGAGCAGGGT GAGCTGGAGC GGCTGTCCAT TCCTGACTTC 
CTGCGGCCAC TGCAGGACCT GGAGGTGGGA CTGGCCAAGG AGGCCATGCT AGAGTGCCAG 
GTGACCGGCC TGCCCTACCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATCCAGAGC 
AGCGACGACC GGCGCATGAC ACAGTACAGG GATGTCCATC GCTTGGTGTT CCCTGCCGTG 
GGGCCTCAGC ACGCCGGTGT CTACAAGAGC GTCATTGCCA ACAAGCTGGG CAAAGCTGCC 
TGCTATGCCC ACCTGTATGT CACAGATGTG GTCCCAGGCC CTCCAGATGG CGCCCCGCAG 
GTGGTGGCTG TGACGGGGAG GATGGTCACA CTCACATGGA ACCCCCCCAG GAGTCTGGAC 
ATGGCCATCG ACCCGGACTC CCTGACGTAC ACAGTGCAGC ACCAGGTGCT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCAGGGT GGGCAGCCAC AGGGCTGCGT 
AAGGGGGTCC AGCACATCTT CCGGGTCCTC AGCACCACTG TCAAGAGCAG CAGCAAGCCC 
TCACCCCCTT CTGAGCCTGT GCAGCTGCTG GAGCACGGCC CAACCCTGGA GGAGGCCCCT 
GCCATGCTGG ACAAACCAGA CATCGTGTAT GTGGTGGAGG GACAGCCTGC CAGCGTCACC 
GTCACATTCA ACCATGTGGA GGCCCAGGTC GTCTGGAGGA GCTGCCGAGG GGCCCTCCTA 
GAGGCACGGG CCGGTGTGTA CGAGCTGAGC CAGCCAGATG ATGACCAGTA CTGTCTTCGG 
ATCTGCCGGG TGAGCCGCCG GGACATGGGG GCCCTCACCT GCACCGCCCG AAACCGTCAC 
GGCACACAGA CCTGCTCGGT CACATTGGAG CTGGCAGAGG CCCCTCGGTT TGAGTCCATC 
ATGGAGGACG TGGAGGTGGG GGCTGGGGAA ACTGCTCGCT TTGCGGTGGT GGTCGAGGGA 
AAACCACTGC CGGACATCAT GTGGTACAAG GACGAGGTGC TGCTGACCGA GAGCAGCCAT 
GTGAGCTTCG TGTACGAGGA GAATGAGTGC TCCCTGGTGG TGCTCAGCAC GGGGGCCCAG 
GATGGAGGCG TCTACACCTG CACCGCCCAG AACCTGGCGG GTGAGGTCTC CTGCAAAGCA 
GAGTTGGCTG TGCATTCAGC TCAGACAGCT ATGGAGGTCG AGGGGGTCGG GGAGGATGAG 
GACCATCGAG GAAGGAGACT CAGCGACTTT TATGACATCC ACCAGGAGAT CGGCAGGGGT 
GCTTTCTCCT ACTTGCGGCG CATAGTGGAG CGTAGCTCCG GCCTGGAGTT TGCGGCCAAG 
TTCATCCCCA GCCAGGCCAA GCCAAAGGCA TCAGCGCGTC GGGAGGCCCG GCTGCTGGCC 
AGGCTCCAGC ACGACTGTGT CCTCTACTTC CATGAGGCCT TCGAGAGGCG CCGGGGACTG 
GTCATTGTCA CCGAGCTCTG CACAGAGGAG CTGCTGGAGC GAATCGCCAG GAAACCCACC 
GTGTGTGAGT CTGAGATCCG GGCCTATATG CGGCAGGTGC TAGAGGGAAT ACACTACCTG 
CACCAGAGCC ACGTGCTGCA CCTCGATGTC AAGCCTGAGA ACCTGCTGGT GTGGGATGGT 
GCTGCGGGCG AGCAGCAGGT GCGGATCTGT GACTTTGGGA ATGCCCAGGA GCTGACTCCA 
GGAGAGCCCC AGTACTGCCA GTATGGCACA CCTGAGTTTG TAGCACCCGA GATTGTCAAT 
CAGAGCCCCG TGTCTGGAGT CACTGACATC TGGCCTGTGG GTGTTGTTGC CTTCCTCTGT 
CTGACAGGAA TCTCCCCGTT TGTTGGGGAA AATGACCGGA CAACATTGAT GAACATCCGA 
AACTACAACG TGGCCTTCGA GGAGACCACA TTCCTGAGCC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGTTGGT GCAGGACCGG CTGAGACCTA CCGCAGAAGA GACCCTAGAA 
CATCCTTGGT TCAAAACTCA GGCAAAGGGC GCAGAGGTGA GCACGGATCA CCTGAAGCTA 
TTCCTCTCCC GGCGGAGGTG GCAGCGCTCC CAGATCAGCT ACAAATGCCA CCTGGTGCTG 
CGCCCCATCC CCGAGCTGCT GCGGGCCCCC CCAGAGCGGG TGTGGGTGAC CATGCCCAGA 
AGGCCACCCC CCAGTGGGGG GCTCTCATCC TCCTCGGATT CTGAAGAGGA AGAGCTGGAA 
GAGCTGCCCT CAGTGCCCCG CCCACTGCAG CCCGAGTTCT CTGGCTCCCG GGTGTCCCTC 
ACAGACATTC CCACTGAGGA TGAGGCCCTG GGGACCCCAG AGACTGGGGC TGCCACCCCC 
ATGGACTGGC AGGAGCAGGG AAGGGCTCCC TCTCAGGACC AGGAGGCTCC CAGCCCAGAG 
GCCCTCCCCT CCCCAGGCCA GGAGCCCGCA GCTGGGGCTA GCCCCAGGCG GGGAGAGCTC 
CGCAGGGGCA GCTCGGCTGA GAGCGCCCTG CCCCGGGCCG GGCCGCGGGA GCTGGGCCGG 
GGCCTGCACA AGGCGGCGTC TGTGGAGCTG CCGCAGCGCC GGAGCCCCAG CCCGGGAGCC 
ACCCGCCTGG CCCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGCCAGCGGC TGCTGCGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGTCCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCTCGG GACCCCCGGA TGGCACGAGC TGCCTCCAGC 
GAGGCAGCGC CCCACCACCA GCCCCCACTC GAGAACCGGG GCCTGCAAAA GAGCAGCAGC 
TTCTCCCAGG GTGAGGCGGA GCCCCGGGGC CGGCACCGCC GAGCGGGGGC GCCCCTCGAG 
ATCCCCGTGG CCAGGCTTGG GGCCCGTAGG CTACAGGAGT CTCCTTCCCT GTCTGCCCTC 
AGCGAGGCCC AGCCATCCAG CCCTGCACGG CCCAGCGCCC CCAAACCCAG TACCCCTAAG 
TCTGCAGAAC CTTCTGCCAC CACACCTAGT GATGCTCCGC AGCCCCCCGC ACCCCAGCCT 
GCCCAAGACA AGGCTCCAGA GCCCAGGCCA GAACCAGTCC GAGCCTCCAA GCCTGCACCA 
CCCCCCCAGG CCCTGCAAAC CCTAGCGCTG CCCCTCACAC CCTATGCTCA GATCATTCAG 
TCCCTCCAGC TGTCAGGCCA CGCCCAGGGC CCCTCGCAGG GCCCTGCCGC GCCGCCTTCA 
GAGCCCAAGC CCCACGCTGC TGTCTTTGCC AGGGTGGCCT CCCCACCTCC GGGAGCCCCC 
GAGAAGCGCG TGCCCTCAGC CGGGGGTCCC CCGGTGCTAG CCGAGAAAGC CCGAGTTCCC 
ACGGTGCCCC CCAGGCCAGG CAGCAGTCTC AGTAGCAGCA TCGAAAACTT GGAGTCGGAG 
GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC CGCGAGTCGC CCCTGTCGCT GGGGCTGCGG 
CTGCTGAGCC GTTCGCGCTC GGAGGAGCGC GGCCCCTTCC GTGGGGCCGA GGAGGAGGAT 
GGCATATACC GGCCCAGCCC GGCGGGGACC CCGCTGGAGC TGGTGCGACG GCCTGAGCGC 
TCACGCTCGG TGCAGGACCT CAGGGCTGTC GGAGAGCCTG GCCTCGTCCG CCGCCTCTCG 
CTGTCACTGT CCCAGCGGCT GCGGCGGACC CCTCCCGCGC AGCGCCACCC GGCCTGGGAG 
GCCCGCGGCG GGGACGGAGA GAGCTCGGAG GGCGGGAGCT CGGCGCGGGG CTCCCCGGTG 
CTGGCGATGC GCAGGCGGCT GAGCTTCACC CTGGAGCGGC TGTCCAGCCG ATTGCAGCGC 
AGTGGCAGCA GCGAGGACTC GGGGGGCGCG TCGGGCCGCA GCACGCCGCT GTTCGGACGG 
CTTCGCAGGG CCACGTCCGA GGGCGAGAGT CTGCGGCGCC TTGGCCTTCC GCACAACCAG 
TTGGCCGCCC AGGCCGGCGC CACCACGCCT TCCGCCGAGT CCCTGGGCTC CGAGGCCAGC 
GCCACGTCGG GCTCCTCAGC CCCAGGGGAA AGCCGAAGCC GGCTCCGCTG GGGCTTCTCT 
CGGCCGCGGA AGGACAAGGG GTTATCGCCA CCAAACCTCT CTGCCAGCGT CCAGGAGGAG 
TTGGGTCACC AGTACGTGCG CAGTGAGTCA GACTTCCCCC CAGTCTTCCA CATCAAACTC 
AAGGACCAGG TGCTGCTGGA GGGGGAGGCA GCCACCCTGC TCTGCCTGCC AGCGGCCTGC 
CCTGCACCGC ACATCTCCTG GATGAAAGAC AAGAAGTCCT TGAGGTCAGA GCCCTCAGTG 
ATCATCGTGT CCTGCAAAGA TGGGCGGCAG CTGCTCAGCA TCCCCCGGGC GGGCAAGCGG 
CACGCCGGTC TCTATGAGTG CTCGGCCACC AACGTACTGG GCAGCATCAC CAGCTCCTGT 
ACCGTGGCTG TGGCCCGAGT CCCAGGAAAG CTAGCTCCTC CAGAGGTACC CCAGACCTAC 
CAGGACACGG CGCTGGTGCT GTGGAAGCCG GGAGACAGCC GGGCACCTTG CACGTATACG 
CTGGAGCGGC GAGTGGATGG GGAGTCTGTG TGGCACCCTG TGAGCTCAGG CATCCCCGAC 
TGTTACTACA ACGTGACCCA CCTGCCAGTT GGCGTGACTG TGAGGTTCCG TGTGGCCTGT 
GCCAACCGTG CTGGGCAGGG GCCCTTCAGC AACTCTTCTG AGAAGGTCTT TGTCAGGGGT 
ACTCAAGATT CTTCAGCTGT GCCATCTGCT GCCCACCAAG AGGCCCCTGT CACCTCAAGG 
CCAGCCAGGG CCCGGCCTCC TGACTCTCCT ACCTCACTGG CCCCACCCCT AGCTCCTGCT 
GCCCCCACAC CCCCGTCAGT CACTGTCAGC CCCTCATCTC CCCCCACACC TCCTAGCCAG 
GCCTTGTCCT CGCTCAAGGC TGTGGGTCCA CCACCCCAAA CCCCTCCACG AAGACACAGG 
GGCCTGCAGG CTGCCCGGCC AGCGGAGCCC ACCCTACCCA GTACCCACGT CACCCCAAGT 
GAGCCCAAGC CTTTCGTCCT TGACACTGGG ACCCCGATCC CAGCCTCCAC TCCTCAAGGG 
GTTAAACCAG TGTCTTCCTC TACTCCTGTG TATGTGGTGA CTTCCTTTGT GTCTGCACCA 
CCAGCCCCTG AGCCCCCAGC CCCTGAGCCC CCTCCTGAGC CTACCAAGGT GACTGTGCAG 
AGCCTCAGCC CGGCCAAGGA GGTGGTCAGC TCCCCTGGGA GCAGTCCCCG AAGCTCTCCC 
AGGCCTGAGG GTACCACTCT TCGACAGGGT CCCCCTCAGA AACCCTACAC CTTCCTGGAG 
GAGAAAGCCA GGGGCCGCTT TGGTGTTGTG CGAGCGTGCC GGGAGAATGC CACGGGGCGA 
ACGTTCGTGG CCAAGATCGT GCCCTATGCT GCCGAGGGCA AGCGGCGGGT CCTGCAGGAG 
TACGAGGTGC TGCGGACCCT GCACCACGAG CGGATCATGT CCCTGCACGA GGCCTACATC 
ACCCCTCGGT ACCTCGTGCT CATTGCTGAG AGCTGTGGCA ACCGGGAACT CCTCTGTGGG 
CTCAGTGACA GGTTCCGGTA TTCTGAGGAT GACGTGGCCA CTTACATGGT GCAGCTGCTA 
CAAGGCCTGG ACTACCTCCA CGGCCACCAC GTGCTCCACC TAGACATCAA GCCAGACAAC 
CTGCTGCTGG CCCCTGACAA TGCCCTCAAG ATTGTGGACT TTGGCAGTGC CCAGCCCTAC 
AACCCCCAGG CCCTTAGGCC CCTTGGCCAC CGCACGGGCA CGCTGGAGTT CATGGCTCCG 
GAGATGGTGA AGGGAGAACC CATCGGCTCT GCCACGGACA TCTGGGGAGC GGGTGTGCTC 
ACTTACATTA TGCTCAGTGG ACGCTCCCCG TTCTATGAGC CAGACCCCCA GGAAACGGAG 
GCTCGGATTG TGGGGGGCCG CTTTGATGCC TTCCAGCTGT ACCCCAATAC ATCCCAGAGC 
GCCACCCTCT TCTTGCGAAA GGTTCTCTCT GTACATCCCT GGAGCCGGCC CTCCCTGCAG 
GACTGCCTGG CCCACCCATG GTTGCAGGAC GCCTACCTGA TGAAGCTGCG CCGCCAGACG 
CTCACCTTCA CCACCAACCG GCTCAAGGAG TTCCTGGGCG AGCAGCGGCG GCGCCGGGCT 
GAGGCTGCCA CCCGCCACAA GGTGCTGCTG CGCTCCTACC CTGGCGGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508781.1
CDS	137..9970
Translation	MQKARGTRGEDAGTRAPPSPGVPPKRAKVGAGGGAPVAVAGAPVFLRPLKNAAVCAGSDVRLRVVVSGTPQPSLRWFRDGQLLPAPAPEPSCLWLRRCGAQDAGVYSCMAQNERGRASCEAVLTVLEVGVFRSCRKQSYDSETAEDDISDVQGTQRLELRDDGAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRRLPGSPRQAQATGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSVPQSGLRREEPDLQPQLASEAPRRPAQPPPSKSALLPPPSPRVGKRSPPGPPAQPAATPTSPHRRTQEPVLPEDTTTEEKRGKKSKSSGPSLAGTAESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSERGAPWGTPGASQEELRAPGSVAERRRLFQQKAASLDERTRQRSPASDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAAAQPPSPSSAEKPGDEPGRPRSRGPAGRTEPGEGPQQEVRRRDQFPLTRSRAIQECRSPVPPPAADPPEARTKAPPGRKREPPAQAVRFLPWATPGLEGAAVPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGARSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNVVVAPGADVLLKCIITANPPPQVSWHKDGSALRSEGRLLLRAEGERHTLLLREARAADAGSYMATATNELGQATCAASLTVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPTDVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARFDCKISGTPPPVVTWTHFGCPMEESENLRLRQDGGLHSLHIAHVGSEDEGLYAVSAVNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEQGELERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDVHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPQVVAVTGRMVTLTWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPGWAATGLRKGVQHIFRVLSTTVKSSSKPSPPSEPVQLLEHGPTLEEAPAMLDKPDIVYVVEGQPASVTVTFNHVEAQVVWRSCRGALLEARAGVYELSQPDDDQYCLRICRVSRRDMGALTCTARNRHGTQTCSVTLELAEAPRFESIMEDVEVGAGETARFAVVVEGKPLPDIMWYKDEVLLTESSHVSFVYEENECSLVVLSTGAQDGGVYTCTAQNLAGEVSCKAELAVHSAQTAMEVEGVGEDEDHRGRRLSDFYDIHQEIGRGAFSYLRRIVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHDCVLYFHEAFERRRGLVIVTELCTEELLERIARKPTVCESEIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTQAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGELRRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQPPAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPSEPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEAKFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAVGEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAESLGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPHISWMKDKKSLRSEPSVIIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARVPGKLAPPEVPQTYQDTALVLWKPGDSRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTHLPVGVTVRFRVACANRAGQGPFSNSSEKVFVRGTQDSSAVPSAAHQEAPVTSRPARARPPDSPTSLAPPLAPAAPTPPSVTVSPSSPPTPPSQALSSLKAVGPPPQTPPRRHRGLQAARPAEPTLPSTHVTPSEPKPFVLDTGTPIPASTPQGVKPVSSSTPVYVVTSFVSAPPAPEPPAPEPPPEPTKVTVQSLSPAKEVVSSPGSSPRSSPRPEGTTLRQGPPQKPYTFLEEKARGRFGVVRACRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERIMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYMVQLLQGLDYLHGHHVLHLDIKPDNLLLAPDNALKIVDFGSAQPYNPQALRPLGHRTGTLEFMAPEMVKGEPIGSATDIWGAGVLTYIMLSGRSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGGP

Target ORF information:

RefSeq Version	XM_011510479.2
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510479.2

ORF Insert Sequence:

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GATGCGGGCA CGAGGGCACC CCCCAGCCCC 
GGAGTGCCCC CGAAAAGGGC CAAGGTGGGG GCCGGCGGCG GGGCTCCTGT GGCCGTGGCC 
GGGGCGCCAG TCTTCCTGCG GCCCCTGAAG AACGCGGCGG TGTGCGCGGG CAGCGACGTG 
CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCCGCTGGTT CCGGGATGGG 
CAGCTCCTGC CCGCGCCGGC CCCCGAGCCC AGCTGCCTGT GGCTGCGGCG CTGCGGGGCG 
CAGGACGCCG GCGTGTACAG CTGCATGGCC CAGAACGAGC GGGGCCGGGC CTCCTGCGAG 
GCGGTGCTCA CAGTGCTGGA GGTCGGAGTT TTCCGCTCCT GCAGAAAGCA AAGTTACGAC 
TCAGAGACGG CTGAGGATGA CATCAGCGAT GTGCAGGGAA CCCAGCGCCT GGAGCTTCGG 
GATGACGGGG CCTTCAGCAC CCCCACGGGG GGTTCTGACA CCCTGGTGGG CACCTCCCTG 
GACACACCCC CGACCTCCGT GACAGGCACC TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC 
GGGCAGACGG TCCTGGAGCA GGAAGCGGGC AGTGGGGGTG GCACCCGCCG CCTCCCGGGC 
AGCCCAAGGC AAGCACAGGC AACCGGGGCC GGGCCACGGC ACCTGGGGGT GGAGCCGCTG 
GTGCGGGCAT CTCGAGCTAA TCTGGTGGGC GCAAGCTGGG GGTCAGAGGA TAGCCTTTCC 
GTGGCCAGTG ACCTGTACGG CAGCGCATTC AGCCTGTACA GAGGACGGGC GCTCTCTATC 
CACGTCAGCG TCCCTCAGAG CGGGTTGCGC AGGGAGGAGC CCGACCTTCA GCCTCAACTG 
GCCAGCGAAG CCCCACGCCG CCCTGCCCAG CCGCCTCCTT CCAAATCCGC GCTGCTCCCC 
CCACCGTCCC CTCGGGTCGG GAAGCGGTCC CCGCCGGGAC CCCCGGCCCA GCCCGCGGCC 
ACCCCCACGT CGCCCCACCG TCGCACTCAG GAGCCTGTGC TGCCCGAGGA CACCACCACC 
GAAGAGAAGC GAGGGAAGAA GTCCAAGTCG TCCGGGCCCT CCCTGGCGGG CACCGCGGAA 
TCCCGACCCC AGACGCCACT GAGCGAGGCC TCAGGCCGCC TGTCGGCGTT GGGCCGATCG 
CCTAGGCTGG TGCGCGCCGG CTCCCGCATC CTGGACAAGC TGCAGTTCTT CGAGGAGCGA 
CGGCGCAGCC TGGAGCGCAG CGACTCGCCG CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG 
CGCAAGGCCC GCTCTCTGGA GCAGCCCAAG TCGGAGCGCG GCGCACCGTG GGGCACCCCC 
GGGGCCTCGC AGGAAGAACT GCGGGCGCCA GGCAGCGTGG CCGAGCGGCG CCGCCTGTTC 
CAGCAGAAAG CGGCCTCGCT GGACGAGCGC ACGCGTCAGC GCAGCCCGGC CTCAGACCTC 
GAGCTGCGCT TCGCCCAGGA GCTGGGCCGC ATCCGCCGCT CCACGTCGCG GGAGGAGCTG 
GTGCGCTCGC ACGAGTCCCT GCGCGCCACG CTGCAGCGTG CCCCATCCCC TCGAGAGCCC 
GGCGAGCCCC CGCTCTTCTC TCGGCCCTCC ACCCCCAAGA CATCGCGGGC CGTGAGCCCC 
GCCGCCGCCC AGCCGCCCTC TCCGAGCAGC GCGGAGAAGC CGGGGGACGA GCCTGGGAGG 
CCCAGGAGCC GCGGGCCGGC GGGCAGGACA GAGCCGGGGG AAGGCCCGCA GCAGGAGGTT 
AGGCGTCGGG ACCAATTCCC GCTGACCCGG AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT 
GTGCCGCCCC CCGCCGCCGA TCCCCCAGAG GCCAGGACGA AAGCACCCCC CGGTCGGAAG 
CGGGAGCCCC CGGCGCAGGC CGTGCGCTTC CTGCCCTGGG CCACGCCGGG CCTGGAGGGC 
GCTGCTGTAC CCCAGACCTT GGAGAAGAAC AGGGCGGGGC CTGAGGCAGA GAAGAGGCTT 
CGCAGAGGGC CGGAGGAGGA CGGTCCCTGG GGGCCCTGGG ACCGCCGAGG GGCCCGCAGC 
CAGGGCAAAG GTCGCCGGGC CCGGCCCACC TCCCCTGAGC TCGAGTCTTC GGATGACTCC 
TACGTGTCCG CTGGAGAAGA GCCCCTAGAG GCCCCTGTGT TTGAGATCCC CCTGCAGAAT 
GTGGTGGTGG CACCAGGGGC AGATGTGCTG CTCAAGTGTA TCATCACTGC CAACCCCCCG 
CCCCAAGTGT CCTGGCACAA GGATGGGTCA GCGCTGCGCA GCGAGGGCCG CCTCCTCCTC 
CGGGCTGAGG GTGAGCGGCA CACCCTGCTG CTCAGGGAGG CCAGGGCAGC AGATGCCGGG 
AGCTATATGG CCACCGCCAC CAACGAGCTG GGCCAGGCCA CCTGTGCCGC CTCACTGACC 
GTGAGACCCG GTGGGTCTAC ATCCCCTTTC AGCAGCCCCA TCACCTCCGA CGAGGAATAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCGCGAAC CCCCACCATG 
AAGCCCAGTC CCAGCCAGAA CCGCCGTTCT TCTGACACTG GCTCCAAGGC ACCCCCCACC 
TTCAAGGTCT CACTTATGGA CCAGTCAGTA AGAGAAGGCC AAGATGTCAT CATGAGCATC 
CGCGTGCAGG GGGAGCCCAA GCCTGTGGTC TCCTGGCTGA GAAACCGCCA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC GGAGGAGGCT GAGGGTGGGC TGTGCCGGCT GCGGATCCTG 
GCTGCAGAGC GTGGCGATGC TGGTTTCTAC ACTTGCAAAG CGGTCAATGA GTATGGTGCT 
CGGCAGTGCG AGGCCCGCTT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCGGGGC CCACTGACGT GGAGGTGGAT TGGCTGTGCC GTGGCCGCCT GCTGCAGCCT 
GCACTGCTCA AATGCAAGAT GCATTTCGAT GGCCGCAAAT GCAAGCTGCT ACTTACATCT 
GTACATGAGG ACGACAGTGG CGTCTACACC TGCAAGCTCA GCACGGCCAA AGATGAGCTG 
ACCTGCAGTG CCCGGCTGAC CGTGCGGCCC TCGTTGGCAC CCCTGTTCAC ACGGCTGCTG 
GAAGATGTGG AGGTGTTGGA GGGCCGAGCT GCCCGTTTCG ACTGCAAGAT CAGTGGCACC 
CCGCCCCCTG TTGTTACCTG GACTCATTTT GGCTGCCCCA TGGAGGAGAG TGAGAACTTG 
CGGCTGCGGC AGGACGGGGG TCTGCACTCA CTGCACATTG CCCATGTGGG CAGCGAGGAC 
GAGGGGCTCT ATGCGGTCAG TGCTGTTAAC ACCCATGGCC AGGCCCACTG CTCAGCCCAG 
CTGTATGTAG AAGAGCCCCG GACAGCCGCC TCAGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCCATTC CCGAGGAGCC AGAGCAGGGT GAGCTGGAGC GGCTGTCCAT TCCTGACTTC 
CTGCGGCCAC TGCAGGACCT GGAGGTGGGA CTGGCCAAGG AGGCCATGCT AGAGTGCCAG 
GTGACCGGCC TGCCCTACCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATCCAGAGC 
AGCGACGACC GGCGCATGAC ACAGTACAGG GATGTCCATC GCTTGGTGTT CCCTGCCGTG 
GGGCCTCAGC ACGCCGGTGT CTACAAGAGC GTCATTGCCA ACAAGCTGGG CAAAGCTGCC 
TGCTATGCCC ACCTGTATGT CACAGATGTG GTCCCAGGCC CTCCAGATGG CGCCCCGCAG 
GTGGTGGCTG TGACGGGGAG GATGGTCACA CTCACATGGA ACCCCCCCAG GAGTCTGGAC 
ATGGCCATCG ACCCGGACTC CCTGACGTAC ACAGTGCAGC ACCAGGTGCT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCAGGGT GGGCAGCCAC AGGGCTGCGT 
AAGGGGGTCC AGCACATCTT CCGGGTCCTC AGCACCACTG TCAAGAGCAG CAGCAAGCCC 
TCACCCCCTT CTGAGCCTGT GCAGCTGCTG GAGCACGGCC CAACCCTGGA GGAGGCCCCT 
GCCATGCTGG ACAAACCAGA CATCGTGTAT GTGGTGGAGG GACAGCCTGC CAGCGTCACC 
GTCACATTCA ACCATGTGGA GGCCCAGGTC GTCTGGAGGA GCTGCCGAGG GGCCCTCCTA 
GAGGCACGGG CCGGTGTGTA CGAGCTGAGC CAGCCAGATG ATGACCAGTA CTGTCTTCGG 
ATCTGCCGGG TGAGCCGCCG GGACATGGGG GCCCTCACCT GCACCGCCCG AAACCGTCAC 
GGCACACAGA CCTGCTCGGT CACATTGGAG CTGGCAGAGG CCCCTCGGTT TGAGTCCATC 
ATGGAGGACG TGGAGGTGGG GGCTGGGGAA ACTGCTCGCT TTGCGGTGGT GGTCGAGGGA 
AAACCACTGC CGGACATCAT GTGGTACAAG GACGAGGTGC TGCTGACCGA GAGCAGCCAT 
GTGAGCTTCG TGTACGAGGA GAATGAGTGC TCCCTGGTGG TGCTCAGCAC GGGGGCCCAG 
GATGGAGGCG TCTACACCTG CACCGCCCAG AACCTGGCGG GTGAGGTCTC CTGCAAAGCA 
GAGTTGGCTG TGCATTCAGC TCAGACAGCT ATGGAGGTCG AGGGGGTCGG GGAGGATGAG 
GACCATCGAG GAAGGAGACT CAGCGACTTT TATGACATCC ACCAGGAGAT CGGCAGGGGT 
GCTTTCTCCT ACTTGCGGCG CATAGTGGAG CGTAGCTCCG GCCTGGAGTT TGCGGCCAAG 
TTCATCCCCA GCCAGGCCAA GCCAAAGGCA TCAGCGCGTC GGGAGGCCCG GCTGCTGGCC 
AGGCTCCAGC ACGACTGTGT CCTCTACTTC CATGAGGCCT TCGAGAGGCG CCGGGGACTG 
GTCATTGTCA CCGAGCTCTG CACAGAGGAG CTGCTGGAGC GAATCGCCAG GAAACCCACC 
GTGTGTGAGT CTGAGATCCG GGCCTATATG CGGCAGGTGC TAGAGGGAAT ACACTACCTG 
CACCAGAGCC ACGTGCTGCA CCTCGATGTC AAGCCTGAGA ACCTGCTGGT GTGGGATGGT 
GCTGCGGGCG AGCAGCAGGT GCGGATCTGT GACTTTGGGA ATGCCCAGGA GCTGACTCCA 
GGAGAGCCCC AGTACTGCCA GTATGGCACA CCTGAGTTTG TAGCACCCGA GATTGTCAAT 
CAGAGCCCCG TGTCTGGAGT CACTGACATC TGGCCTGTGG GTGTTGTTGC CTTCCTCTGT 
CTGACAGGAA TCTCCCCGTT TGTTGGGGAA AATGACCGGA CAACATTGAT GAACATCCGA 
AACTACAACG TGGCCTTCGA GGAGACCACA TTCCTGAGCC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGTTGGT GCAGGACCGG CTGAGACCTA CCGCAGAAGA GACCCTAGAA 
CATCCTTGGT TCAAAACTCA GGCAAAGGGC GCAGAGGTGA GCACGGATCA CCTGAAGCTA 
TTCCTCTCCC GGCGGAGGTG GCAGCGCTCC CAGATCAGCT ACAAATGCCA CCTGGTGCTG 
CGCCCCATCC CCGAGCTGCT GCGGGCCCCC CCAGAGCGGG TGTGGGTGAC CATGCCCAGA 
AGGCCACCCC CCAGTGGGGG GCTCTCATCC TCCTCGGATT CTGAAGAGGA AGAGCTGGAA 
GAGCTGCCCT CAGTGCCCCG CCCACTGCAG CCCGAGTTCT CTGGCTCCCG GGTGTCCCTC 
ACAGACATTC CCACTGAGGA TGAGGCCCTG GGGACCCCAG AGACTGGGGC TGCCACCCCC 
ATGGACTGGC AGGAGCAGGG AAGGGCTCCC TCTCAGGACC AGGAGGCTCC CAGCCCAGAG 
GCCCTCCCCT CCCCAGGCCA GGAGCCCGCA GCTGGGGCTA GCCCCAGGCG GGGAGAGCTC 
CGCAGGGGCA GCTCGGCTGA GAGCGCCCTG CCCCGGGCCG GGCCGCGGGA GCTGGGCCGG 
GGCCTGCACA AGGCGGCGTC TGTGGAGCTG CCGCAGCGCC GGAGCCCCAG CCCGGGAGCC 
ACCCGCCTGG CCCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGCCAGCGGC TGCTGCGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGTCCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCTCGG GACCCCCGGA TGGCACGAGC TGCCTCCAGC 
GAGGCAGCGC CCCACCACCA GCCCCCACTC GAGAACCGGG GCCTGCAAAA GAGCAGCAGC 
TTCTCCCAGG GTGAGGCGGA GCCCCGGGGC CGGCACCGCC GAGCGGGGGC GCCCCTCGAG 
ATCCCCGTGG CCAGGCTTGG GGCCCGTAGG CTACAGGAGT CTCCTTCCCT GTCTGCCCTC 
AGCGAGGCCC AGCCATCCAG CCCTGCACGG CCCAGCGCCC CCAAACCCAG TACCCCTAAG 
TCTGCAGAAC CTTCTGCCAC CACACCTAGT GATGCTCCGC AGCCCCCCGC ACCCCAGCCT 
GCCCAAGACA AGGCTCCAGA GCCCAGGCCA GAACCAGTCC GAGCCTCCAA GCCTGCACCA 
CCCCCCCAGG CCCTGCAAAC CCTAGCGCTG CCCCTCACAC CCTATGCTCA GATCATTCAG 
TCCCTCCAGC TGTCAGGCCA CGCCCAGGGC CCCTCGCAGG GCCCTGCCGC GCCGCCTTCA 
GAGCCCAAGC CCCACGCTGC TGTCTTTGCC AGGGTGGCCT CCCCACCTCC GGGAGCCCCC 
GAGAAGCGCG TGCCCTCAGC CGGGGGTCCC CCGGTGCTAG CCGAGAAAGC CCGAGTTCCC 
ACGGTGCCCC CCAGGCCAGG CAGCAGTCTC AGTAGCAGCA TCGAAAACTT GGAGTCGGAG 
GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC CGCGAGTCGC CCCTGTCGCT GGGGCTGCGG 
CTGCTGAGCC GTTCGCGCTC GGAGGAGCGC GGCCCCTTCC GTGGGGCCGA GGAGGAGGAT 
GGCATATACC GGCCCAGCCC GGCGGGGACC CCGCTGGAGC TGGTGCGACG GCCTGAGCGC 
TCACGCTCGG TGCAGGACCT CAGGGCTGTC GGAGAGCCTG GCCTCGTCCG CCGCCTCTCG 
CTGTCACTGT CCCAGCGGCT GCGGCGGACC CCTCCCGCGC AGCGCCACCC GGCCTGGGAG 
GCCCGCGGCG GGGACGGAGA GAGCTCGGAG GGCGGGAGCT CGGCGCGGGG CTCCCCGGTG 
CTGGCGATGC GCAGGCGGCT GAGCTTCACC CTGGAGCGGC TGTCCAGCCG ATTGCAGCGC 
AGTGGCAGCA GCGAGGACTC GGGGGGCGCG TCGGGCCGCA GCACGCCGCT GTTCGGACGG 
CTTCGCAGGG CCACGTCCGA GGGCGAGAGT CTGCGGCGCC TTGGCCTTCC GCACAACCAG 
TTGGCCGCCC AGGCCGGCGC CACCACGCCT TCCGCCGAGT CCCTGGGCTC CGAGGCCAGC 
GCCACGTCGG GCTCCTCAGC CCCAGGGGAA AGCCGAAGCC GGCTCCGCTG GGGCTTCTCT 
CGGCCGCGGA AGGACAAGGG GTTATCGCCA CCAAACCTCT CTGCCAGCGT CCAGGAGGAG 
TTGGGTCACC AGTACGTGCG CAGTGAGTCA GACTTCCCCC CAGTCTTCCA CATCAAACTC 
AAGGACCAGG TGCTGCTGGA GGGGGAGGCA GCCACCCTGC TCTGCCTGCC AGCGGCCTGC 
CCTGCACCGC ACATCTCCTG GATGAAAGAC AAGAAGTCCT TGAGGTCAGA GCCCTCAGTG 
ATCATCGTGT CCTGCAAAGA TGGGCGGCAG CTGCTCAGCA TCCCCCGGGC GGGCAAGCGG 
CACGCCGGTC TCTATGAGTG CTCGGCCACC AACGTACTGG GCAGCATCAC CAGCTCCTGT 
ACCGTGGCTG TGGCCCGAGT CCCAGGAAAG CTAGCTCCTC CAGAGGTACC CCAGACCTAC 
CAGGACACGG CGCTGGTGCT GTGGAAGCCG GGAGACAGCC GGGCACCTTG CACGTATACG 
CTGGAGCGGC GAGTGGATGG GGAGTCTGTG TGGCACCCTG TGAGCTCAGG CATCCCCGAC 
TGTTACTACA ACGTGACCCA CCTGCCAGTT GGCGTGACTG TGAGGTTCCG TGTGGCCTGT 
GCCAACCGTG CTGGGCAGGG GCCCTTCAGC AACTCTTCTG AGAAGGTCTT TGTCAGGGGT 
ACTCAAGATT CTTCAGCTGT GCCATCTGCT GCCCACCAAG AGGCCCCTGT CACCTCAAGG 
CCAGCCAGGG CCCGGCCTCC TGACTCTCCT ACCTCACTGG CCCCACCCCT AGCTCCTGCT 
GCCCCCACAC CCCCGTCAGT CACTGTCAGC CCCTCATCTC CCCCCACACC TCCTAGCCAG 
GCCTTGTCCT CGCTCAAGGC TGTGGGTCCA CCACCCCAAA CCCCTCCACG AAGACACAGG 
GGCCTGCAGG CTGCCCGGCC AGCGGAGCCC ACCCTACCCA GTACCCACGT CACCCCAAGT 
GAGCCCAAGC CTTTCGTCCT TGACACTGGG ACCCCGATCC CAGCCTCCAC TCCTCAAGGG 
GTTAAACCAG TGTCTTCCTC TACTCCTGTG TATGTGGTGA CTTCCTTTGT GTCTGCACCA 
CCAGCCCCTG AGCCCCCAGC CCCTGAGCCC CCTCCTGAGC CTACCAAGGT GACTGTGCAG 
AGCCTCAGCC CGGCCAAGGA GGTGGTCAGC TCCCCTGGGA GCAGTCCCCG AAGCTCTCCC 
AGGCCTGAGG GTACCACTCT TCGACAGGGT CCCCCTCAGA AACCCTACAC CTTCCTGGAG 
GAGAAAGCCA GGGGCCGCTT TGGTGTTGTG CGAGCGTGCC GGGAGAATGC CACGGGGCGA 
ACGTTCGTGG CCAAGATCGT GCCCTATGCT GCCGAGGGCA AGCGGCGGGT CCTGCAGGAG 
TACGAGGTGC TGCGGACCCT GCACCACGAG CGGATCATGT CCCTGCACGA GGCCTACATC 
ACCCCTCGGT ACCTCGTGCT CATTGCTGAG AGCTGTGGCA ACCGGGAACT CCTCTGTGGG 
CTCAGTGACA GGTTCCGGTA TTCTGAGGAT GACGTGGCCA CTTACATGGT GCAGCTGCTA 
CAAGGCCTGG ACTACCTCCA CGGCCACCAC GTGCTCCACC TAGACATCAA GCCAGACAAC 
CTGCTGCTGG CCCCTGACAA TGCCCTCAAG ATTGTGGACT TTGGCAGTGC CCAGCCCTAC 
AACCCCCAGG CCCTTAGGCC CCTTGGCCAC CGCACGGGCA CGCTGGAGTT CATGGCTCCG 
GAGATGGTGA AGGGAGAACC CATCGGCTCT GCCACGGACA TCTGGGGAGC GGGTGTGCTC 
ACTTACATTA TGCTCAGTGG ACGCTCCCCG TTCTATGAGC CAGACCCCCA GGAAACGGAG 
GCTCGGATTG TGGGGGGCCG CTTTGATGCC TTCCAGCTGT ACCCCAATAC ATCCCAGAGC 
GCCACCCTCT TCTTGCGAAA GGTTCTCTCT GTACATCCCT GGAGCCGGCC CTCCCTGCAG 
GACTGCCTGG CCCACCCATG GTTGCAGGAC GCCTACCTGA TGAAGCTGCG CCGCCAGACG 
CTCACCTTCA CCACCAACCG GCTCAAGGAG TTCCTGGGCG AGCAGCGGCG GCGCCGGGCT 
GAGGCTGCCA CCCGCCACAA GGTGCTGCTG CGCTCCTACC CTGGCGGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_011510483.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508785.2 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X1, mRNA.

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CloneID	OHu88918
Clone ID Related Accession (Same CDS sequence)	XM_011510483.2
Accession Version	XM_011510483.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510483.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTGCC CACTCTTTTC TGCCTATTGC AACCCTATTC CACCACCCAT TCTTACAGAT 
GAGGACTTGG AGGTTGAGAG AGGTTTGGTG TCACCCAGCA ACGAAGTTTT CCGCTCCTGC 
AGAAAGCAAA GTTACGACTC AGAGACGGCT GAGGATGACA TCAGCGATGT GCAGGGAACC 
CAGCGCCTGG AGCTTCGGGA TGACGGGGCC TTCAGCACCC CCACGGGGGG TTCTGACACC 
CTGGTGGGCA CCTCCCTGGA CACACCCCCG ACCTCCGTGA CAGGCACCTC AGAGGAGCAA 
GTGAGCTGGT GGGGCAGCGG GCAGACGGTC CTGGAGCAGG AAGCGGGCAG TGGGGGTGGC 
ACCCGCCGCC TCCCGGGCAG CCCAAGGCAA GCACAGGCAA CCGGGGCCGG GCCACGGCAC 
CTGGGGGTGG AGCCGCTGGT GCGGGCATCT CGAGCTAATC TGGTGGGCGC AAGCTGGGGG 
TCAGAGGATA GCCTTTCCGT GGCCAGTGAC CTGTACGGCA GCGCATTCAG CCTGTACAGA 
GGACGGGCGC TCTCTATCCA CGTCAGCGTC CCTCAGAGCG GGTTGCGCAG GGAGGAGCCC 
GACCTTCAGC CTCAACTGGC CAGCGAAGCC CCACGCCGCC CTGCCCAGCC GCCTCCTTCC 
AAATCCGCGC TGCTCCCCCC ACCGTCCCCT CGGGTCGGGA AGCGGTCCCC GCCGGGACCC 
CCGGCCCAGC CCGCGGCCAC CCCCACGTCG CCCCACCGTC GCACTCAGGA GCCTGTGCTG 
CCCGAGGACA CCACCACCGA AGAGAAGCGA GGGAAGAAGT CCAAGTCGTC CGGGCCCTCC 
CTGGCGGGCA CCGCGGAATC CCGACCCCAG ACGCCACTGA GCGAGGCCTC AGGCCGCCTG 
TCGGCGTTGG GCCGATCGCC TAGGCTGGTG CGCGCCGGCT CCCGCATCCT GGACAAGCTG 
CAGTTCTTCG AGGAGCGACG GCGCAGCCTG GAGCGCAGCG ACTCGCCGCC GGCGCCCCTG 
CGGCCCTGGG TGCCCCTGCG CAAGGCCCGC TCTCTGGAGC AGCCCAAGTC GGAGCGCGGC 
GCACCGTGGG GCACCCCCGG GGCCTCGCAG GAAGAACTGC GGGCGCCAGG CAGCGTGGCC 
GAGCGGCGCC GCCTGTTCCA GCAGAAAGCG GCCTCGCTGG ACGAGCGCAC GCGTCAGCGC 
AGCCCGGCCT CAGACCTCGA GCTGCGCTTC GCCCAGGAGC TGGGCCGCAT CCGCCGCTCC 
ACGTCGCGGG AGGAGCTGGT GCGCTCGCAC GAGTCCCTGC GCGCCACGCT GCAGCGTGCC 
CCATCCCCTC GAGAGCCCGG CGAGCCCCCG CTCTTCTCTC GGCCCTCCAC CCCCAAGACA 
TCGCGGGCCG TGAGCCCCGC CGCCGCCCAG CCGCCCTCTC CGAGCAGCGC GGAGAAGCCG 
GGGGACGAGC CTGGGAGGCC CAGGAGCCGC GGGCCGGCGG GCAGGACAGA GCCGGGGGAA 
GGCCCGCAGC AGGAGGTTAG GCGTCGGGAC CAATTCCCGC TGACCCGGAG CAGAGCCATC 
CAGGAGTGCA GGAGCCCTGT GCCGCCCCCC GCCGCCGATC CCCCAGAGGC CAGGACGAAA 
GCACCCCCCG GTCGGAAGCG GGAGCCCCCG GCGCAGGCCG TGCGCTTCCT GCCCTGGGCC 
ACGCCGGGCC TGGAGGGCGC TGCTGTACCC CAGACCTTGG AGAAGAACAG GGCGGGGCCT 
GAGGCAGAGA AGAGGCTTCG CAGAGGGCCG GAGGAGGACG GTCCCTGGGG GCCCTGGGAC 
CGCCGAGGGG CCCGCAGCCA GGGCAAAGGT CGCCGGGCCC GGCCCACCTC CCCTGAGCTC 
GAGTCTTCGG ATGACTCCTA CGTGTCCGCT GGAGAAGAGC CCCTAGAGGC CCCTGTGTTT 
GAGATCCCCC TGCAGAATGT GGTGGTGGCA CCAGGGGCAG ATGTGCTGCT CAAGTGTATC 
ATCACTGCCA ACCCCCCGCC CCAAGTGTCC TGGCACAAGG ATGGGTCAGC GCTGCGCAGC 
GAGGGCCGCC TCCTCCTCCG GGCTGAGGGT GAGCGGCACA CCCTGCTGCT CAGGGAGGCC 
AGGGCAGCAG ATGCCGGGAG CTATATGGCC ACCGCCACCA ACGAGCTGGG CCAGGCCACC 
TGTGCCGCCT CACTGACCGT GAGACCCGGT GGGTCTACAT CCCCTTTCAG CAGCCCCATC 
ACCTCCGACG AGGAATACCT GAGCCCCCCA GAGGAGTTCC CAGAGCCTGG GGAGACCTGG 
CCGCGAACCC CCACCATGAA GCCCAGTCCC AGCCAGAACC GCCGTTCTTC TGACACTGGC 
TCCAAGGCAC CCCCCACCTT CAAGGTCTCA CTTATGGACC AGTCAGTAAG AGAAGGCCAA 
GATGTCATCA TGAGCATCCG CGTGCAGGGG GAGCCCAAGC CTGTGGTCTC CTGGCTGAGA 
AACCGCCAGC CCGTGCGCCC AGACCAGCGG CGCTTTGCGG AGGAGGCTGA GGGTGGGCTG 
TGCCGGCTGC GGATCCTGGC TGCAGAGCGT GGCGATGCTG GTTTCTACAC TTGCAAAGCG 
GTCAATGAGT ATGGTGCTCG GCAGTGCGAG GCCCGCTTGG AGGTCCGAGC ACACCCTGAA 
AGCCGGTCCC TGGCCGTGCT GGCCCCCCTG CAGGACGTGG ACGTGGGGGC CGGGGAGATG 
GCGCTGTTTG AGTGCCTGGT GGCGGGGCCC ACTGACGTGG AGGTGGATTG GCTGTGCCGT 
GGCCGCCTGC TGCAGCCTGC ACTGCTCAAA TGCAAGATGC ATTTCGATGG CCGCAAATGC 
AAGCTGCTAC TTACATCTGT ACATGAGGAC GACAGTGGCG TCTACACCTG CAAGCTCAGC 
ACGGCCAAAG ATGAGCTGAC CTGCAGTGCC CGGCTGACCG TGCGGCCCTC GTTGGCACCC 
CTGTTCACAC GGCTGCTGGA AGATGTGGAG GTGTTGGAGG GCCGAGCTGC CCGTTTCGAC 
TGCAAGATCA GTGGCACCCC GCCCCCTGTT GTTACCTGGA CTCATTTTGG CTGCCCCATG 
GAGGAGAGTG AGAACTTGCG GCTGCGGCAG GACGGGGGTC TGCACTCACT GCACATTGCC 
CATGTGGGCA GCGAGGACGA GGGGCTCTAT GCGGTCAGTG CTGTTAACAC CCATGGCCAG 
GCCCACTGCT CAGCCCAGCT GTATGTAGAA GAGCCCCGGA CAGCCGCCTC AGGCCCCAGC 
TCGAAGCTGG AGAAGATGCC ATCCATTCCC GAGGAGCCAG AGCAGGGTGA GCTGGAGCGG 
CTGTCCATTC CTGACTTCCT GCGGCCACTG CAGGACCTGG AGGTGGGACT GGCCAAGGAG 
GCCATGCTAG AGTGCCAGGT GACCGGCCTG CCCTACCCCA CCATCAGCTG GTTCCACAAT 
GGCCACCGCA TCCAGAGCAG CGACGACCGG CGCATGACAC AGTACAGGGA TGTCCATCGC 
TTGGTGTTCC CTGCCGTGGG GCCTCAGCAC GCCGGTGTCT ACAAGAGCGT CATTGCCAAC 
AAGCTGGGCA AAGCTGCCTG CTATGCCCAC CTGTATGTCA CAGATGTGGT CCCAGGCCCT 
CCAGATGGCG CCCCGCAGGT GGTGGCTGTG ACGGGGAGGA TGGTCACACT CACATGGAAC 
CCCCCCAGGA GTCTGGACAT GGCCATCGAC CCGGACTCCC TGACGTACAC AGTGCAGCAC 
CAGGTGCTGG GCTCGGACCA GTGGACGGCA CTGGTCACAG GCCTGCGGGA GCCAGGGTGG 
GCAGCCACAG GGCTGCGTAA GGGGGTCCAG CACATCTTCC GGGTCCTCAG CACCACTGTC 
AAGAGCAGCA GCAAGCCCTC ACCCCCTTCT GAGCCTGTGC AGCTGCTGGA GCACGGCCCA 
ACCCTGGAGG AGGCCCCTGC CATGCTGGAC AAACCAGACA TCGTGTATGT GGTGGAGGGA 
CAGCCTGCCA GCGTCACCGT CACATTCAAC CATGTGGAGG CCCAGGTCGT CTGGAGGAGC 
TGCCGAGGGG CCCTCCTAGA GGCACGGGCC GGTGTGTACG AGCTGAGCCA GCCAGATGAT 
GACCAGTACT GTCTTCGGAT CTGCCGGGTG AGCCGCCGGG ACATGGGGGC CCTCACCTGC 
ACCGCCCGAA ACCGTCACGG CACACAGACC TGCTCGGTCA CATTGGAGCT GGCAGAGGCC 
CCTCGGTTTG AGTCCATCAT GGAGGACGTG GAGGTGGGGG CTGGGGAAAC TGCTCGCTTT 
GCGGTGGTGG TCGAGGGAAA ACCACTGCCG GACATCATGT GGTACAAGGA CGAGGTGCTG 
CTGACCGAGA GCAGCCATGT GAGCTTCGTG TACGAGGAGA ATGAGTGCTC CCTGGTGGTG 
CTCAGCACGG GGGCCCAGGA TGGAGGCGTC TACACCTGCA CCGCCCAGAA CCTGGCGGGT 
GAGGTCTCCT GCAAAGCAGA GTTGGCTGTG CATTCAGCTC AGACAGCTAT GGAGGTCGAG 
GGGGTCGGGG AGGATGAGGA CCATCGAGGA AGGAGACTCA GCGACTTTTA TGACATCCAC 
CAGGAGATCG GCAGGGGTGC TTTCTCCTAC TTGCGGCGCA TAGTGGAGCG TAGCTCCGGC 
CTGGAGTTTG CGGCCAAGTT CATCCCCAGC CAGGCCAAGC CAAAGGCATC AGCGCGTCGG 
GAGGCCCGGC TGCTGGCCAG GCTCCAGCAC GACTGTGTCC TCTACTTCCA TGAGGCCTTC 
GAGAGGCGCC GGGGACTGGT CATTGTCACC GAGCTCTGCA CAGAGGAGCT GCTGGAGCGA 
ATCGCCAGGA AACCCACCGT GTGTGAGTCT GAGATCCGGG CCTATATGCG GCAGGTGCTA 
GAGGGAATAC ACTACCTGCA CCAGAGCCAC GTGCTGCACC TCGATGTCAA GCCTGAGAAC 
CTGCTGGTGT GGGATGGTGC TGCGGGCGAG CAGCAGGTGC GGATCTGTGA CTTTGGGAAT 
GCCCAGGAGC TGACTCCAGG AGAGCCCCAG TACTGCCAGT ATGGCACACC TGAGTTTGTA 
GCACCCGAGA TTGTCAATCA GAGCCCCGTG TCTGGAGTCA CTGACATCTG GCCTGTGGGT 
GTTGTTGCCT TCCTCTGTCT GACAGGAATC TCCCCGTTTG TTGGGGAAAA TGACCGGACA 
ACATTGATGA ACATCCGAAA CTACAACGTG GCCTTCGAGG AGACCACATT CCTGAGCCTG 
AGCAGGGAGG CCCGGGGCTT CCTCATCAAA GTGTTGGTGC AGGACCGGCT GAGACCTACC 
GCAGAAGAGA CCCTAGAACA TCCTTGGTTC AAAACTCAGG CAAAGGGCGC AGAGGTGAGC 
ACGGATCACC TGAAGCTATT CCTCTCCCGG CGGAGGTGGC AGCGCTCCCA GATCAGCTAC 
AAATGCCACC TGGTGCTGCG CCCCATCCCC GAGCTGCTGC GGGCCCCCCC AGAGCGGGTG 
TGGGTGACCA TGCCCAGAAG GCCACCCCCC AGTGGGGGGC TCTCATCCTC CTCGGATTCT 
GAAGAGGAAG AGCTGGAAGA GCTGCCCTCA GTGCCCCGCC CACTGCAGCC CGAGTTCTCT 
GGCTCCCGGG TGTCCCTCAC AGACATTCCC ACTGAGGATG AGGCCCTGGG GACCCCAGAG 
ACTGGGGCTG CCACCCCCAT GGACTGGCAG GAGCAGGGAA GGGCTCCCTC TCAGGACCAG 
GAGGCTCCCA GCCCAGAGGC CCTCCCCTCC CCAGGCCAGG AGCCCGCAGC TGGGGCTAGC 
CCCAGGCGGG GAGAGCTCCG CAGGGGCAGC TCGGCTGAGA GCGCCCTGCC CCGGGCCGGG 
CCGCGGGAGC TGGGCCGGGG CCTGCACAAG GCGGCGTCTG TGGAGCTGCC GCAGCGCCGG 
AGCCCCAGCC CGGGAGCCAC CCGCCTGGCC CGGGGAGGCC TGGGTGAGGG CGAGTATGCC 
CAGAGGCTGC AGGCCCTGCG CCAGCGGCTG CTGCGGGGAG GCCCCGAGGA TGGCAAGGTC 
AGCGGCCTCA GGGGTCCCCT GCTGGAGAGC CTGGGGGGCC GTGCTCGGGA CCCCCGGATG 
GCACGAGCTG CCTCCAGCGA GGCAGCGCCC CACCACCAGC CCCCACTCGA GAACCGGGGC 
CTGCAAAAGA GCAGCAGCTT CTCCCAGGGT GAGGCGGAGC CCCGGGGCCG GCACCGCCGA 
GCGGGGGCGC CCCTCGAGAT CCCCGTGGCC AGGCTTGGGG CCCGTAGGCT ACAGGAGTCT 
CCTTCCCTGT CTGCCCTCAG CGAGGCCCAG CCATCCAGCC CTGCACGGCC CAGCGCCCCC 
AAACCCAGTA CCCCTAAGTC TGCAGAACCT TCTGCCACCA CACCTAGTGA TGCTCCGCAG 
CCCCCCGCAC CCCAGCCTGC CCAAGACAAG GCTCCAGAGC CCAGGCCAGA ACCAGTCCGA 
GCCTCCAAGC CTGCACCACC CCCCCAGGCC CTGCAAACCC TAGCGCTGCC CCTCACACCC 
TATGCTCAGA TCATTCAGTC CCTCCAGCTG TCAGGCCACG CCCAGGGCCC CTCGCAGGGC 
CCTGCCGCGC CGCCTTCAGA GCCCAAGCCC CACGCTGCTG TCTTTGCCAG GGTGGCCTCC 
CCACCTCCGG GAGCCCCCGA GAAGCGCGTG CCCTCAGCCG GGGGTCCCCC GGTGCTAGCC 
GAGAAAGCCC GAGTTCCCAC GGTGCCCCCC AGGCCAGGCA GCAGTCTCAG TAGCAGCATC 
GAAAACTTGG AGTCGGAGGC CGTGTTCGAG GCCAAGTTCA AGCGCAGCCG CGAGTCGCCC 
CTGTCGCTGG GGCTGCGGCT GCTGAGCCGT TCGCGCTCGG AGGAGCGCGG CCCCTTCCGT 
GGGGCCGAGG AGGAGGATGG CATATACCGG CCCAGCCCGG CGGGGACCCC GCTGGAGCTG 
GTGCGACGGC CTGAGCGCTC ACGCTCGGTG CAGGACCTCA GGGCTGTCGG AGAGCCTGGC 
CTCGTCCGCC GCCTCTCGCT GTCACTGTCC CAGCGGCTGC GGCGGACCCC TCCCGCGCAG 
CGCCACCCGG CCTGGGAGGC CCGCGGCGGG GACGGAGAGA GCTCGGAGGG CGGGAGCTCG 
GCGCGGGGCT CCCCGGTGCT GGCGATGCGC AGGCGGCTGA GCTTCACCCT GGAGCGGCTG 
TCCAGCCGAT TGCAGCGCAG TGGCAGCAGC GAGGACTCGG GGGGCGCGTC GGGCCGCAGC 
ACGCCGCTGT TCGGACGGCT TCGCAGGGCC ACGTCCGAGG GCGAGAGTCT GCGGCGCCTT 
GGCCTTCCGC ACAACCAGTT GGCCGCCCAG GCCGGCGCCA CCACGCCTTC CGCCGAGTCC 
CTGGGCTCCG AGGCCAGCGC CACGTCGGGC TCCTCAGCCC CAGGGGAAAG CCGAAGCCGG 
CTCCGCTGGG GCTTCTCTCG GCCGCGGAAG GACAAGGGGT TATCGCCACC AAACCTCTCT 
GCCAGCGTCC AGGAGGAGTT GGGTCACCAG TACGTGCGCA GTGAGTCAGA CTTCCCCCCA 
GTCTTCCACA TCAAACTCAA GGACCAGGTG CTGCTGGAGG GGGAGGCAGC CACCCTGCTC 
TGCCTGCCAG CGGCCTGCCC TGCACCGCAC ATCTCCTGGA TGAAAGACAA GAAGTCCTTG 
AGGTCAGAGC CCTCAGTGAT CATCGTGTCC TGCAAAGATG GGCGGCAGCT GCTCAGCATC 
CCCCGGGCGG GCAAGCGGCA CGCCGGTCTC TATGAGTGCT CGGCCACCAA CGTACTGGGC 
AGCATCACCA GCTCCTGTAC CGTGGCTGTG GCCCGAGTCC CAGGAAAGCT AGCTCCTCCA 
GAGGTACCCC AGACCTACCA GGACACGGCG CTGGTGCTGT GGAAGCCGGG AGACAGCCGG 
GCACCTTGCA CGTATACGCT GGAGCGGCGA GTGGATGGGG AGTCTGTGTG GCACCCTGTG 
AGCTCAGGCA TCCCCGACTG TTACTACAAC GTGACCCACC TGCCAGTTGG CGTGACTGTG 
AGGTTCCGTG TGGCCTGTGC CAACCGTGCT GGGCAGGGGC CCTTCAGCAA CTCTTCTGAG 
AAGGTCTTTG TCAGGGGTAC TCAAGATTCT TCAGCTGTGC CATCTGCTGC CCACCAAGAG 
GCCCCTGTCA CCTCAAGGCC AGCCAGGGCC CGGCCTCCTG ACTCTCCTAC CTCACTGGCC 
CCACCCCTAG CTCCTGCTGC CCCCACACCC CCGTCAGTCA CTGTCAGCCC CTCATCTCCC 
CCCACACCTC CTAGCCAGGC CTTGTCCTCG CTCAAGGCTG TGGGTCCACC ACCCCAAACC 
CCTCCACGAA GACACAGGGG CCTGCAGGCT GCCCGGCCAG CGGAGCCCAC CCTACCCAGT 
ACCCACGTCA CCCCAAGTGA GCCCAAGCCT TTCGTCCTTG ACACTGGGAC CCCGATCCCA 
GCCTCCACTC CTCAAGGGGT TAAACCAGTG TCTTCCTCTA CTCCTGTGTA TGTGGTGACT 
TCCTTTGTGT CTGCACCACC AGCCCCTGAG CCCCCAGCCC CTGAGCCCCC TCCTGAGCCT 
ACCAAGGTGA CTGTGCAGAG CCTCAGCCCG GCCAAGGAGG TGGTCAGCTC CCCTGGGAGC 
AGTCCCCGAA GCTCTCCCAG GCCTGAGGGT ACCACTCTTC GACAGGGTCC CCCTCAGAAA 
CCCTACACCT TCCTGGAGGA GAAAGCCAGG GGCCGCTTTG GTGTTGTGCG AGCGTGCCGG 
GAGAATGCCA CGGGGCGAAC GTTCGTGGCC AAGATCGTGC CCTATGCTGC CGAGGGCAAG 
CGGCGGGTCC TGCAGGAGTA CGAGGTGCTG CGGACCCTGC ACCACGAGCG GATCATGTCC 
CTGCACGAGG CCTACATCAC CCCTCGGTAC CTCGTGCTCA TTGCTGAGAG CTGTGGCAAC 
CGGGAACTCC TCTGTGGGCT CAGTGACAGG TTCCGGTATT CTGAGGATGA CGTGGCCACT 
TACATGGTGC AGCTGCTACA AGGCCTGGAC TACCTCCACG GCCACCACGT GCTCCACCTA 
GACATCAAGC CAGACAACCT GCTGCTGGCC CCTGACAATG CCCTCAAGAT TGTGGACTTT 
GGCAGTGCCC AGCCCTACAA CCCCCAGGCC CTTAGGCCCC TTGGCCACCG CACGGGCACG 
CTGGAGTTCA TGGCTCCGGA GATGGTGAAG GGAGAACCCA TCGGCTCTGC CACGGACATC 
TGGGGAGCGG GTGTGCTCAC TTACATTATG CTCAGTGGAC GCTCCCCGTT CTATGAGCCA 
GACCCCCAGG AAACGGAGGC TCGGATTGTG GGGGGCCGCT TTGATGCCTT CCAGCTGTAC 
CCCAATACAT CCCAGAGCGC CACCCTCTTC TTGCGAAAGG TTCTCTCTGT ACATCCCTGG 
AGCCGGCCCT CCCTGCAGGA CTGCCTGGCC CACCCATGGT TGCAGGACGC CTACCTGATG 
AAGCTGCGCC GCCAGACGCT CACCTTCACC ACCAACCGGC TCAAGGAGTT CCTGGGCGAG 
CAGCGGCGGC GCCGGGCTGA GGCTGCCACC CGCCACAAGG TGCTGCTGCG CTCCTACCCT 
GGCGGCCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508785.2
CDS	16..9567
Translation	MSCPLFSAYCNPIPPPILTDEDLEVERGLVSPSNEVFRSCRKQSYDSETAEDDISDVQGTQRLELRDDGAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRRLPGSPRQAQATGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSVPQSGLRREEPDLQPQLASEAPRRPAQPPPSKSALLPPPSPRVGKRSPPGPPAQPAATPTSPHRRTQEPVLPEDTTTEEKRGKKSKSSGPSLAGTAESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSERGAPWGTPGASQEELRAPGSVAERRRLFQQKAASLDERTRQRSPASDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAAAQPPSPSSAEKPGDEPGRPRSRGPAGRTEPGEGPQQEVRRRDQFPLTRSRAIQECRSPVPPPAADPPEARTKAPPGRKREPPAQAVRFLPWATPGLEGAAVPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGARSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNVVVAPGADVLLKCIITANPPPQVSWHKDGSALRSEGRLLLRAEGERHTLLLREARAADAGSYMATATNELGQATCAASLTVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPTDVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARFDCKISGTPPPVVTWTHFGCPMEESENLRLRQDGGLHSLHIAHVGSEDEGLYAVSAVNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEQGELERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDVHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPQVVAVTGRMVTLTWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPGWAATGLRKGVQHIFRVLSTTVKSSSKPSPPSEPVQLLEHGPTLEEAPAMLDKPDIVYVVEGQPASVTVTFNHVEAQVVWRSCRGALLEARAGVYELSQPDDDQYCLRICRVSRRDMGALTCTARNRHGTQTCSVTLELAEAPRFESIMEDVEVGAGETARFAVVVEGKPLPDIMWYKDEVLLTESSHVSFVYEENECSLVVLSTGAQDGGVYTCTAQNLAGEVSCKAELAVHSAQTAMEVEGVGEDEDHRGRRLSDFYDIHQEIGRGAFSYLRRIVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHDCVLYFHEAFERRRGLVIVTELCTEELLERIARKPTVCESEIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTQAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGELRRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQPPAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPSEPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEAKFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAVGEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAESLGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPHISWMKDKKSLRSEPSVIIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARVPGKLAPPEVPQTYQDTALVLWKPGDSRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTHLPVGVTVRFRVACANRAGQGPFSNSSEKVFVRGTQDSSAVPSAAHQEAPVTSRPARARPPDSPTSLAPPLAPAAPTPPSVTVSPSSPPTPPSQALSSLKAVGPPPQTPPRRHRGLQAARPAEPTLPSTHVTPSEPKPFVLDTGTPIPASTPQGVKPVSSSTPVYVVTSFVSAPPAPEPPAPEPPPEPTKVTVQSLSPAKEVVSSPGSSPRSSPRPEGTTLRQGPPQKPYTFLEEKARGRFGVVRACRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERIMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYMVQLLQGLDYLHGHHVLHLDIKPDNLLLAPDNALKIVDFGSAQPYNPQALRPLGHRTGTLEFMAPEMVKGEPIGSATDIWGAGVLTYIMLSGRSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGGP

Target ORF information:

RefSeq Version	XM_011510483.2
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510483.2

ORF Insert Sequence:

ATGTCCTGCC CACTCTTTTC TGCCTATTGC AACCCTATTC CACCACCCAT TCTTACAGAT 
GAGGACTTGG AGGTTGAGAG AGGTTTGGTG TCACCCAGCA ACGAAGTTTT CCGCTCCTGC 
AGAAAGCAAA GTTACGACTC AGAGACGGCT GAGGATGACA TCAGCGATGT GCAGGGAACC 
CAGCGCCTGG AGCTTCGGGA TGACGGGGCC TTCAGCACCC CCACGGGGGG TTCTGACACC 
CTGGTGGGCA CCTCCCTGGA CACACCCCCG ACCTCCGTGA CAGGCACCTC AGAGGAGCAA 
GTGAGCTGGT GGGGCAGCGG GCAGACGGTC CTGGAGCAGG AAGCGGGCAG TGGGGGTGGC 
ACCCGCCGCC TCCCGGGCAG CCCAAGGCAA GCACAGGCAA CCGGGGCCGG GCCACGGCAC 
CTGGGGGTGG AGCCGCTGGT GCGGGCATCT CGAGCTAATC TGGTGGGCGC AAGCTGGGGG 
TCAGAGGATA GCCTTTCCGT GGCCAGTGAC CTGTACGGCA GCGCATTCAG CCTGTACAGA 
GGACGGGCGC TCTCTATCCA CGTCAGCGTC CCTCAGAGCG GGTTGCGCAG GGAGGAGCCC 
GACCTTCAGC CTCAACTGGC CAGCGAAGCC CCACGCCGCC CTGCCCAGCC GCCTCCTTCC 
AAATCCGCGC TGCTCCCCCC ACCGTCCCCT CGGGTCGGGA AGCGGTCCCC GCCGGGACCC 
CCGGCCCAGC CCGCGGCCAC CCCCACGTCG CCCCACCGTC GCACTCAGGA GCCTGTGCTG 
CCCGAGGACA CCACCACCGA AGAGAAGCGA GGGAAGAAGT CCAAGTCGTC CGGGCCCTCC 
CTGGCGGGCA CCGCGGAATC CCGACCCCAG ACGCCACTGA GCGAGGCCTC AGGCCGCCTG 
TCGGCGTTGG GCCGATCGCC TAGGCTGGTG CGCGCCGGCT CCCGCATCCT GGACAAGCTG 
CAGTTCTTCG AGGAGCGACG GCGCAGCCTG GAGCGCAGCG ACTCGCCGCC GGCGCCCCTG 
CGGCCCTGGG TGCCCCTGCG CAAGGCCCGC TCTCTGGAGC AGCCCAAGTC GGAGCGCGGC 
GCACCGTGGG GCACCCCCGG GGCCTCGCAG GAAGAACTGC GGGCGCCAGG CAGCGTGGCC 
GAGCGGCGCC GCCTGTTCCA GCAGAAAGCG GCCTCGCTGG ACGAGCGCAC GCGTCAGCGC 
AGCCCGGCCT CAGACCTCGA GCTGCGCTTC GCCCAGGAGC TGGGCCGCAT CCGCCGCTCC 
ACGTCGCGGG AGGAGCTGGT GCGCTCGCAC GAGTCCCTGC GCGCCACGCT GCAGCGTGCC 
CCATCCCCTC GAGAGCCCGG CGAGCCCCCG CTCTTCTCTC GGCCCTCCAC CCCCAAGACA 
TCGCGGGCCG TGAGCCCCGC CGCCGCCCAG CCGCCCTCTC CGAGCAGCGC GGAGAAGCCG 
GGGGACGAGC CTGGGAGGCC CAGGAGCCGC GGGCCGGCGG GCAGGACAGA GCCGGGGGAA 
GGCCCGCAGC AGGAGGTTAG GCGTCGGGAC CAATTCCCGC TGACCCGGAG CAGAGCCATC 
CAGGAGTGCA GGAGCCCTGT GCCGCCCCCC GCCGCCGATC CCCCAGAGGC CAGGACGAAA 
GCACCCCCCG GTCGGAAGCG GGAGCCCCCG GCGCAGGCCG TGCGCTTCCT GCCCTGGGCC 
ACGCCGGGCC TGGAGGGCGC TGCTGTACCC CAGACCTTGG AGAAGAACAG GGCGGGGCCT 
GAGGCAGAGA AGAGGCTTCG CAGAGGGCCG GAGGAGGACG GTCCCTGGGG GCCCTGGGAC 
CGCCGAGGGG CCCGCAGCCA GGGCAAAGGT CGCCGGGCCC GGCCCACCTC CCCTGAGCTC 
GAGTCTTCGG ATGACTCCTA CGTGTCCGCT GGAGAAGAGC CCCTAGAGGC CCCTGTGTTT 
GAGATCCCCC TGCAGAATGT GGTGGTGGCA CCAGGGGCAG ATGTGCTGCT CAAGTGTATC 
ATCACTGCCA ACCCCCCGCC CCAAGTGTCC TGGCACAAGG ATGGGTCAGC GCTGCGCAGC 
GAGGGCCGCC TCCTCCTCCG GGCTGAGGGT GAGCGGCACA CCCTGCTGCT CAGGGAGGCC 
AGGGCAGCAG ATGCCGGGAG CTATATGGCC ACCGCCACCA ACGAGCTGGG CCAGGCCACC 
TGTGCCGCCT CACTGACCGT GAGACCCGGT GGGTCTACAT CCCCTTTCAG CAGCCCCATC 
ACCTCCGACG AGGAATACCT GAGCCCCCCA GAGGAGTTCC CAGAGCCTGG GGAGACCTGG 
CCGCGAACCC CCACCATGAA GCCCAGTCCC AGCCAGAACC GCCGTTCTTC TGACACTGGC 
TCCAAGGCAC CCCCCACCTT CAAGGTCTCA CTTATGGACC AGTCAGTAAG AGAAGGCCAA 
GATGTCATCA TGAGCATCCG CGTGCAGGGG GAGCCCAAGC CTGTGGTCTC CTGGCTGAGA 
AACCGCCAGC CCGTGCGCCC AGACCAGCGG CGCTTTGCGG AGGAGGCTGA GGGTGGGCTG 
TGCCGGCTGC GGATCCTGGC TGCAGAGCGT GGCGATGCTG GTTTCTACAC TTGCAAAGCG 
GTCAATGAGT ATGGTGCTCG GCAGTGCGAG GCCCGCTTGG AGGTCCGAGC ACACCCTGAA 
AGCCGGTCCC TGGCCGTGCT GGCCCCCCTG CAGGACGTGG ACGTGGGGGC CGGGGAGATG 
GCGCTGTTTG AGTGCCTGGT GGCGGGGCCC ACTGACGTGG AGGTGGATTG GCTGTGCCGT 
GGCCGCCTGC TGCAGCCTGC ACTGCTCAAA TGCAAGATGC ATTTCGATGG CCGCAAATGC 
AAGCTGCTAC TTACATCTGT ACATGAGGAC GACAGTGGCG TCTACACCTG CAAGCTCAGC 
ACGGCCAAAG ATGAGCTGAC CTGCAGTGCC CGGCTGACCG TGCGGCCCTC GTTGGCACCC 
CTGTTCACAC GGCTGCTGGA AGATGTGGAG GTGTTGGAGG GCCGAGCTGC CCGTTTCGAC 
TGCAAGATCA GTGGCACCCC GCCCCCTGTT GTTACCTGGA CTCATTTTGG CTGCCCCATG 
GAGGAGAGTG AGAACTTGCG GCTGCGGCAG GACGGGGGTC TGCACTCACT GCACATTGCC 
CATGTGGGCA GCGAGGACGA GGGGCTCTAT GCGGTCAGTG CTGTTAACAC CCATGGCCAG 
GCCCACTGCT CAGCCCAGCT GTATGTAGAA GAGCCCCGGA CAGCCGCCTC AGGCCCCAGC 
TCGAAGCTGG AGAAGATGCC ATCCATTCCC GAGGAGCCAG AGCAGGGTGA GCTGGAGCGG 
CTGTCCATTC CTGACTTCCT GCGGCCACTG CAGGACCTGG AGGTGGGACT GGCCAAGGAG 
GCCATGCTAG AGTGCCAGGT GACCGGCCTG CCCTACCCCA CCATCAGCTG GTTCCACAAT 
GGCCACCGCA TCCAGAGCAG CGACGACCGG CGCATGACAC AGTACAGGGA TGTCCATCGC 
TTGGTGTTCC CTGCCGTGGG GCCTCAGCAC GCCGGTGTCT ACAAGAGCGT CATTGCCAAC 
AAGCTGGGCA AAGCTGCCTG CTATGCCCAC CTGTATGTCA CAGATGTGGT CCCAGGCCCT 
CCAGATGGCG CCCCGCAGGT GGTGGCTGTG ACGGGGAGGA TGGTCACACT CACATGGAAC 
CCCCCCAGGA GTCTGGACAT GGCCATCGAC CCGGACTCCC TGACGTACAC AGTGCAGCAC 
CAGGTGCTGG GCTCGGACCA GTGGACGGCA CTGGTCACAG GCCTGCGGGA GCCAGGGTGG 
GCAGCCACAG GGCTGCGTAA GGGGGTCCAG CACATCTTCC GGGTCCTCAG CACCACTGTC 
AAGAGCAGCA GCAAGCCCTC ACCCCCTTCT GAGCCTGTGC AGCTGCTGGA GCACGGCCCA 
ACCCTGGAGG AGGCCCCTGC CATGCTGGAC AAACCAGACA TCGTGTATGT GGTGGAGGGA 
CAGCCTGCCA GCGTCACCGT CACATTCAAC CATGTGGAGG CCCAGGTCGT CTGGAGGAGC 
TGCCGAGGGG CCCTCCTAGA GGCACGGGCC GGTGTGTACG AGCTGAGCCA GCCAGATGAT 
GACCAGTACT GTCTTCGGAT CTGCCGGGTG AGCCGCCGGG ACATGGGGGC CCTCACCTGC 
ACCGCCCGAA ACCGTCACGG CACACAGACC TGCTCGGTCA CATTGGAGCT GGCAGAGGCC 
CCTCGGTTTG AGTCCATCAT GGAGGACGTG GAGGTGGGGG CTGGGGAAAC TGCTCGCTTT 
GCGGTGGTGG TCGAGGGAAA ACCACTGCCG GACATCATGT GGTACAAGGA CGAGGTGCTG 
CTGACCGAGA GCAGCCATGT GAGCTTCGTG TACGAGGAGA ATGAGTGCTC CCTGGTGGTG 
CTCAGCACGG GGGCCCAGGA TGGAGGCGTC TACACCTGCA CCGCCCAGAA CCTGGCGGGT 
GAGGTCTCCT GCAAAGCAGA GTTGGCTGTG CATTCAGCTC AGACAGCTAT GGAGGTCGAG 
GGGGTCGGGG AGGATGAGGA CCATCGAGGA AGGAGACTCA GCGACTTTTA TGACATCCAC 
CAGGAGATCG GCAGGGGTGC TTTCTCCTAC TTGCGGCGCA TAGTGGAGCG TAGCTCCGGC 
CTGGAGTTTG CGGCCAAGTT CATCCCCAGC CAGGCCAAGC CAAAGGCATC AGCGCGTCGG 
GAGGCCCGGC TGCTGGCCAG GCTCCAGCAC GACTGTGTCC TCTACTTCCA TGAGGCCTTC 
GAGAGGCGCC GGGGACTGGT CATTGTCACC GAGCTCTGCA CAGAGGAGCT GCTGGAGCGA 
ATCGCCAGGA AACCCACCGT GTGTGAGTCT GAGATCCGGG CCTATATGCG GCAGGTGCTA 
GAGGGAATAC ACTACCTGCA CCAGAGCCAC GTGCTGCACC TCGATGTCAA GCCTGAGAAC 
CTGCTGGTGT GGGATGGTGC TGCGGGCGAG CAGCAGGTGC GGATCTGTGA CTTTGGGAAT 
GCCCAGGAGC TGACTCCAGG AGAGCCCCAG TACTGCCAGT ATGGCACACC TGAGTTTGTA 
GCACCCGAGA TTGTCAATCA GAGCCCCGTG TCTGGAGTCA CTGACATCTG GCCTGTGGGT 
GTTGTTGCCT TCCTCTGTCT GACAGGAATC TCCCCGTTTG TTGGGGAAAA TGACCGGACA 
ACATTGATGA ACATCCGAAA CTACAACGTG GCCTTCGAGG AGACCACATT CCTGAGCCTG 
AGCAGGGAGG CCCGGGGCTT CCTCATCAAA GTGTTGGTGC AGGACCGGCT GAGACCTACC 
GCAGAAGAGA CCCTAGAACA TCCTTGGTTC AAAACTCAGG CAAAGGGCGC AGAGGTGAGC 
ACGGATCACC TGAAGCTATT CCTCTCCCGG CGGAGGTGGC AGCGCTCCCA GATCAGCTAC 
AAATGCCACC TGGTGCTGCG CCCCATCCCC GAGCTGCTGC GGGCCCCCCC AGAGCGGGTG 
TGGGTGACCA TGCCCAGAAG GCCACCCCCC AGTGGGGGGC TCTCATCCTC CTCGGATTCT 
GAAGAGGAAG AGCTGGAAGA GCTGCCCTCA GTGCCCCGCC CACTGCAGCC CGAGTTCTCT 
GGCTCCCGGG TGTCCCTCAC AGACATTCCC ACTGAGGATG AGGCCCTGGG GACCCCAGAG 
ACTGGGGCTG CCACCCCCAT GGACTGGCAG GAGCAGGGAA GGGCTCCCTC TCAGGACCAG 
GAGGCTCCCA GCCCAGAGGC CCTCCCCTCC CCAGGCCAGG AGCCCGCAGC TGGGGCTAGC 
CCCAGGCGGG GAGAGCTCCG CAGGGGCAGC TCGGCTGAGA GCGCCCTGCC CCGGGCCGGG 
CCGCGGGAGC TGGGCCGGGG CCTGCACAAG GCGGCGTCTG TGGAGCTGCC GCAGCGCCGG 
AGCCCCAGCC CGGGAGCCAC CCGCCTGGCC CGGGGAGGCC TGGGTGAGGG CGAGTATGCC 
CAGAGGCTGC AGGCCCTGCG CCAGCGGCTG CTGCGGGGAG GCCCCGAGGA TGGCAAGGTC 
AGCGGCCTCA GGGGTCCCCT GCTGGAGAGC CTGGGGGGCC GTGCTCGGGA CCCCCGGATG 
GCACGAGCTG CCTCCAGCGA GGCAGCGCCC CACCACCAGC CCCCACTCGA GAACCGGGGC 
CTGCAAAAGA GCAGCAGCTT CTCCCAGGGT GAGGCGGAGC CCCGGGGCCG GCACCGCCGA 
GCGGGGGCGC CCCTCGAGAT CCCCGTGGCC AGGCTTGGGG CCCGTAGGCT ACAGGAGTCT 
CCTTCCCTGT CTGCCCTCAG CGAGGCCCAG CCATCCAGCC CTGCACGGCC CAGCGCCCCC 
AAACCCAGTA CCCCTAAGTC TGCAGAACCT TCTGCCACCA CACCTAGTGA TGCTCCGCAG 
CCCCCCGCAC CCCAGCCTGC CCAAGACAAG GCTCCAGAGC CCAGGCCAGA ACCAGTCCGA 
GCCTCCAAGC CTGCACCACC CCCCCAGGCC CTGCAAACCC TAGCGCTGCC CCTCACACCC 
TATGCTCAGA TCATTCAGTC CCTCCAGCTG TCAGGCCACG CCCAGGGCCC CTCGCAGGGC 
CCTGCCGCGC CGCCTTCAGA GCCCAAGCCC CACGCTGCTG TCTTTGCCAG GGTGGCCTCC 
CCACCTCCGG GAGCCCCCGA GAAGCGCGTG CCCTCAGCCG GGGGTCCCCC GGTGCTAGCC 
GAGAAAGCCC GAGTTCCCAC GGTGCCCCCC AGGCCAGGCA GCAGTCTCAG TAGCAGCATC 
GAAAACTTGG AGTCGGAGGC CGTGTTCGAG GCCAAGTTCA AGCGCAGCCG CGAGTCGCCC 
CTGTCGCTGG GGCTGCGGCT GCTGAGCCGT TCGCGCTCGG AGGAGCGCGG CCCCTTCCGT 
GGGGCCGAGG AGGAGGATGG CATATACCGG CCCAGCCCGG CGGGGACCCC GCTGGAGCTG 
GTGCGACGGC CTGAGCGCTC ACGCTCGGTG CAGGACCTCA GGGCTGTCGG AGAGCCTGGC 
CTCGTCCGCC GCCTCTCGCT GTCACTGTCC CAGCGGCTGC GGCGGACCCC TCCCGCGCAG 
CGCCACCCGG CCTGGGAGGC CCGCGGCGGG GACGGAGAGA GCTCGGAGGG CGGGAGCTCG 
GCGCGGGGCT CCCCGGTGCT GGCGATGCGC AGGCGGCTGA GCTTCACCCT GGAGCGGCTG 
TCCAGCCGAT TGCAGCGCAG TGGCAGCAGC GAGGACTCGG GGGGCGCGTC GGGCCGCAGC 
ACGCCGCTGT TCGGACGGCT TCGCAGGGCC ACGTCCGAGG GCGAGAGTCT GCGGCGCCTT 
GGCCTTCCGC ACAACCAGTT GGCCGCCCAG GCCGGCGCCA CCACGCCTTC CGCCGAGTCC 
CTGGGCTCCG AGGCCAGCGC CACGTCGGGC TCCTCAGCCC CAGGGGAAAG CCGAAGCCGG 
CTCCGCTGGG GCTTCTCTCG GCCGCGGAAG GACAAGGGGT TATCGCCACC AAACCTCTCT 
GCCAGCGTCC AGGAGGAGTT GGGTCACCAG TACGTGCGCA GTGAGTCAGA CTTCCCCCCA 
GTCTTCCACA TCAAACTCAA GGACCAGGTG CTGCTGGAGG GGGAGGCAGC CACCCTGCTC 
TGCCTGCCAG CGGCCTGCCC TGCACCGCAC ATCTCCTGGA TGAAAGACAA GAAGTCCTTG 
AGGTCAGAGC CCTCAGTGAT CATCGTGTCC TGCAAAGATG GGCGGCAGCT GCTCAGCATC 
CCCCGGGCGG GCAAGCGGCA CGCCGGTCTC TATGAGTGCT CGGCCACCAA CGTACTGGGC 
AGCATCACCA GCTCCTGTAC CGTGGCTGTG GCCCGAGTCC CAGGAAAGCT AGCTCCTCCA 
GAGGTACCCC AGACCTACCA GGACACGGCG CTGGTGCTGT GGAAGCCGGG AGACAGCCGG 
GCACCTTGCA CGTATACGCT GGAGCGGCGA GTGGATGGGG AGTCTGTGTG GCACCCTGTG 
AGCTCAGGCA TCCCCGACTG TTACTACAAC GTGACCCACC TGCCAGTTGG CGTGACTGTG 
AGGTTCCGTG TGGCCTGTGC CAACCGTGCT GGGCAGGGGC CCTTCAGCAA CTCTTCTGAG 
AAGGTCTTTG TCAGGGGTAC TCAAGATTCT TCAGCTGTGC CATCTGCTGC CCACCAAGAG 
GCCCCTGTCA CCTCAAGGCC AGCCAGGGCC CGGCCTCCTG ACTCTCCTAC CTCACTGGCC 
CCACCCCTAG CTCCTGCTGC CCCCACACCC CCGTCAGTCA CTGTCAGCCC CTCATCTCCC 
CCCACACCTC CTAGCCAGGC CTTGTCCTCG CTCAAGGCTG TGGGTCCACC ACCCCAAACC 
CCTCCACGAA GACACAGGGG CCTGCAGGCT GCCCGGCCAG CGGAGCCCAC CCTACCCAGT 
ACCCACGTCA CCCCAAGTGA GCCCAAGCCT TTCGTCCTTG ACACTGGGAC CCCGATCCCA 
GCCTCCACTC CTCAAGGGGT TAAACCAGTG TCTTCCTCTA CTCCTGTGTA TGTGGTGACT 
TCCTTTGTGT CTGCACCACC AGCCCCTGAG CCCCCAGCCC CTGAGCCCCC TCCTGAGCCT 
ACCAAGGTGA CTGTGCAGAG CCTCAGCCCG GCCAAGGAGG TGGTCAGCTC CCCTGGGAGC 
AGTCCCCGAA GCTCTCCCAG GCCTGAGGGT ACCACTCTTC GACAGGGTCC CCCTCAGAAA 
CCCTACACCT TCCTGGAGGA GAAAGCCAGG GGCCGCTTTG GTGTTGTGCG AGCGTGCCGG 
GAGAATGCCA CGGGGCGAAC GTTCGTGGCC AAGATCGTGC CCTATGCTGC CGAGGGCAAG 
CGGCGGGTCC TGCAGGAGTA CGAGGTGCTG CGGACCCTGC ACCACGAGCG GATCATGTCC 
CTGCACGAGG CCTACATCAC CCCTCGGTAC CTCGTGCTCA TTGCTGAGAG CTGTGGCAAC 
CGGGAACTCC TCTGTGGGCT CAGTGACAGG TTCCGGTATT CTGAGGATGA CGTGGCCACT 
TACATGGTGC AGCTGCTACA AGGCCTGGAC TACCTCCACG GCCACCACGT GCTCCACCTA 
GACATCAAGC CAGACAACCT GCTGCTGGCC CCTGACAATG CCCTCAAGAT TGTGGACTTT 
GGCAGTGCCC AGCCCTACAA CCCCCAGGCC CTTAGGCCCC TTGGCCACCG CACGGGCACG 
CTGGAGTTCA TGGCTCCGGA GATGGTGAAG GGAGAACCCA TCGGCTCTGC CACGGACATC 
TGGGGAGCGG GTGTGCTCAC TTACATTATG CTCAGTGGAC GCTCCCCGTT CTATGAGCCA 
GACCCCCAGG AAACGGAGGC TCGGATTGTG GGGGGCCGCT TTGATGCCTT CCAGCTGTAC 
CCCAATACAT CCCAGAGCGC CACCCTCTTC TTGCGAAAGG TTCTCTCTGT ACATCCCTGG 
AGCCGGCCCT CCCTGCAGGA CTGCCTGGCC CACCCATGGT TGCAGGACGC CTACCTGATG 
AAGCTGCGCC GCCAGACGCT CACCTTCACC ACCAACCGGC TCAAGGAGTT CCTGGGCGAG 
CAGCGGCGGC GCCGGGCTGA GGCTGCCACC CGCCACAAGG TGCTGCTGCG CTCCTACCCT 
GGCGGCCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_017003157.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858646.1 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X2, mRNA.

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CloneID	OHu88919
Clone ID Related Accession (Same CDS sequence)	XM_017003157.1
Accession Version	XM_017003157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTGCC CACTCTTTTC TGCCTATTGC AACCCTATTC CACCACCCAT TCTTACAGAT 
GAGGACTTGG AGGTTGAGAG AGGTTTGGTG TCACCCAGCA ACGAAGTTTT CCGCTCCTGC 
AGAAAGCAAA GTTACGACTC AGAGACGGCT GAGGATGACA TCAGCGATGT GCAGGGAACC 
CAGCGCCTGG AGCTTCGGGA TGACGGGGCC TTCAGCACCC CCACGGGGGG TTCTGACACC 
CTGGTGGGCA CCTCCCTGGA CACACCCCCG ACCTCCGTGA CAGGCACCTC AGAGGAGCAA 
GTGAGCTGGT GGGGCAGCGG GCAGACGGTC CTGGAGCAGG AAGCGGGCAG TGGGGGTGGC 
ACCCGCCGCC TCCCGGGCAG CCCAAGGCAA GCACAGGCAA CCGGGGCCGG GCCACGGCAC 
CTGGGGGTGG AGCCGCTGGT GCGGGCATCT CGAGCTAATC TGGTGGGCGC AAGCTGGGGG 
TCAGAGGATA GCCTTTCCGT GGCCAGTGAC CTGTACGGCA GCGCATTCAG CCTGTACAGA 
GGACGGGCGC TCTCTATCCA CGTCAGCGTC CCTCAGAGCG GGTTGCGCAG GGAGGAGCCC 
GACCTTCAGC CTCAACTGGC CAGCGAAGCC CCACGCCGCC CTGCCCAGCC GCCTCCTTCC 
AAATCCGCGC TGCTCCCCCC ACCGTCCCCT CGGGTCGGGA AGCGGTCCCC GCCGGGACCC 
CCGGCCCAGC CCGCGGCCAC CCCCACGTCG CCCCACCGTC GCACTCAGGA GCCTGTGCTG 
CCCGAGGACA CCACCACCGA AGAGAAGCGA GGGAAGAAGT CCAAGTCGTC CGGGCCCTCC 
CTGGCGGGCA CCGCGGAATC CCGACCCCAG ACGCCACTGA GCGAGGCCTC AGGCCGCCTG 
TCGGCGTTGG GCCGATCGCC TAGGCTGGTG CGCGCCGGCT CCCGCATCCT GGACAAGCTG 
CAGTTCTTCG AGGAGCGACG GCGCAGCCTG GAGCGCAGCG ACTCGCCGCC GGCGCCCCTG 
CGGCCCTGGG TGCCCCTGCG CAAGGCCCGC TCTCTGGAGC AGCCCAAGTC GGAGCGCGGC 
GCACCGTGGG GCACCCCCGG GGCCTCGCAG GAAGAACTGC GGGCGCCAGG CAGCGTGGCC 
GAGCGGCGCC GCCTGTTCCA GCAGAAAGCG GCCTCGCTGG ACGAGCGCAC GCGTCAGCGC 
AGCCCGGCCT CAGACCTCGA GCTGCGCTTC GCCCAGGAGC TGGGCCGCAT CCGCCGCTCC 
ACGTCGCGGG AGGAGCTGGT GCGCTCGCAC GAGTCCCTGC GCGCCACGCT GCAGCGTGCC 
CCATCCCCTC GAGAGCCCGG CGAGCCCCCG CTCTTCTCTC GGCCCTCCAC CCCCAAGACA 
TCGCGGGCCG TGAGCCCCGC CGCCGCCCAG CCGCCCTCTC CGAGCAGCGC GGAGAAGCCG 
GGGGACGAGC CTGGGAGGCC CAGGAGCCGC GGGCCGGCGG GCAGGACAGA GCCGGGGGAA 
GGCCCGCAGC AGGAGGTTAG GCGTCGGGAC CAATTCCCGC TGACCCGGAG CAGAGCCATC 
CAGGAGTGCA GGAGCCCTGT GCCGCCCCCC GCCGCCGATC CCCCAGAGGC CAGGACGAAA 
GCACCCCCCG GTCGGAAGCG GGAGCCCCCG GCGCAGGCCG TGCGCTTCCT GCCCTGGGCC 
ACGCCGGGCC TGGAGGGCGC TGCTGTACCC CAGACCTTGG AGAAGAACAG GGCGGGGCCT 
GAGGCAGAGA AGAGGCTTCG CAGAGGGCCG GAGGAGGACG GTCCCTGGGG GCCCTGGGAC 
CGCCGAGGGG CCCGCAGCCA GGGCAAAGGT CGCCGGGCCC GGCCCACCTC CCCTGAGCTC 
GAGTCTTCGG ATGACTCCTA CGTGTCCGCT GGAGAAGAGC CCCTAGAGGC CCCTGTGTTT 
GAGATCCCCC TGCAGAATGT GGTGGTGGCA CCAGGGGCAG ATGTGCTGCT CAAGTGTATC 
ATCACTGCCA ACCCCCCGCC CCAAGTGTCC TGGCACAAGG ATGGGTCAGC GCTGCGCAGC 
GAGGGCCGCC TCCTCCTCCG GGCTGAGGGT GAGCGGCACA CCCTGCTGCT CAGGGAGGCC 
AGGGCAGCAG ATGCCGGGAG CTATATGGCC ACCGCCACCA ACGAGCTGGG CCAGGCCACC 
TGTGCCGCCT CACTGACCGT GAGACCCGGT GGGTCTACAT CCCCTTTCAG CAGCCCCATC 
ACCTCCGACG AGGAATACCT GAGCCCCCCA GAGGAGTTCC CAGAGCCTGG GGAGACCTGG 
CCGCGAACCC CCACCATGAA GCCCAGTCCC AGCCAGAACC GCCGTTCTTC TGACACTGGC 
TCCAAGGCAC CCCCCACCTT CAAGGTCTCA CTTATGGACC AGTCAGTAAG AGAAGGCCAA 
GATGTCATCA TGAGCATCCG CGTGCAGGGG GAGCCCAAGC CTGTGGTCTC CTGGCTGAGA 
AACCGCCAGC CCGTGCGCCC AGACCAGCGG CGCTTTGCGG AGGAGGCTGA GGGTGGGCTG 
TGCCGGCTGC GGATCCTGGC TGCAGAGCGT GGCGATGCTG GTTTCTACAC TTGCAAAGCG 
GTCAATGAGT ATGGTGCTCG GCAGTGCGAG GCCCGCTTGG AGGTCCGAGC ACACCCTGAA 
AGCCGGTCCC TGGCCGTGCT GGCCCCCCTG CAGGACGTGG ACGTGGGGGC CGGGGAGATG 
GCGCTGTTTG AGTGCCTGGT GGCGGGGCCC ACTGACGTGG AGGTGGATTG GCTGTGCCGT 
GGCCGCCTGC TGCAGCCTGC ACTGCTCAAA TGCAAGATGC ATTTCGATGG CCGCAAATGC 
AAGCTGCTAC TTACATCTGT ACATGAGGAC GACAGTGGCG TCTACACCTG CAAGCTCAGC 
ACGGCCAAAG ATGAGCTGAC CTGCAGTGCC CGGCTGACCG TGCGGCCCTC GTTGGCACCC 
CTGTTCACAC GGCTGCTGGA AGATGTGGAG GTGTTGGAGG GCCGAGCTGC CCGTTTCGAC 
TGCAAGATCA GTGGCACCCC GCCCCCTGTT GTTACCTGGA CTCATTTTGG CTGCCCCATG 
GAGGAGAGTG AGAACTTGCG GCTGCGGCAG GACGGGGGTC TGCACTCACT GCACATTGCC 
CATGTGGGCA GCGAGGACGA GGGGCTCTAT GCGGTCAGTG CTGTTAACAC CCATGGCCAG 
GCCCACTGCT CAGCCCAGCT GTATGTAGAA GAGCCCCGGA CAGCCGCCTC AGGCCCCAGC 
TCGAAGCTGG AGAAGATGCC ATCCATTCCC GAGGAGCCAG AGCAGGGTGA GCTGGAGCGG 
CTGTCCATTC CTGACTTCCT GCGGCCACTG CAGGACCTGG AGGTGGGACT GGCCAAGGAG 
GCCATGCTAG AGTGCCAGGT GACCGGCCTG CCCTACCCCA CCATCAGCTG GTTCCACAAT 
GGCCACCGCA TCCAGAGCAG CGACGACCGG CGCATGACAC AGTACAGGGA TGTCCATCGC 
TTGGTGTTCC CTGCCGTGGG GCCTCAGCAC GCCGGTGTCT ACAAGAGCGT CATTGCCAAC 
AAGCTGGGCA AAGCTGCCTG CTATGCCCAC CTGTATGTCA CAGATGTGGT CCCAGGCCCT 
CCAGATGGCG CCCCGCAGGT GGTGGCTGTG ACGGGGAGGA TGGTCACACT CACATGGAAC 
CCCCCCAGGA GTCTGGACAT GGCCATCGAC CCGGACTCCC TGACGTACAC AGTGCAGCAC 
CAGGTGCTGG GCTCGGACCA GTGGACGGCA CTGGTCACAG GCCTGCGGGA GCCAGGGTGG 
GCAGCCACAG GGCTGCGTAA GGGGGTCCAG CACATCTTCC GGGTCCTCAG CACCACTGTC 
AAGAGCAGCA GCAAGCCCTC ACCCCCTTCT GAGCCTGTGC AGCTGCTGGA GCACGGCCCA 
ACCCTGGAGG AGGCCCCTGC CATGCTGGAC AAACCAGACA TCGTGTATGT GGTGGAGGGA 
CAGCCTGCCA GCGTCACCGT CACATTCAAC CATGTGGAGG CCCAGGTCGT CTGGAGGAGC 
TGCCGAGGGG CCCTCCTAGA GGCACGGGCC GGTGTGTACG AGCTGAGCCA GCCAGATGAT 
GACCAGTACT GTCTTCGGAT CTGCCGGGTG AGCCGCCGGG ACATGGGGGC CCTCACCTGC 
ACCGCCCGAA ACCGTCACGG CACACAGACC TGCTCGGTCA CATTGGAGCT GGCAGAGGCC 
CCTCGGTTTG AGTCCATCAT GGAGGACGTG GAGGTGGGGG CTGGGGAAAC TGCTCGCTTT 
GCGGTGGTGG TCGAGGGAAA ACCACTGCCG GACATCATGT GGTACAAGGA CGAGGTGCTG 
CTGACCGAGA GCAGCCATGT GAGCTTCGTG TACGAGGAGA ATGAGTGCTC CCTGGTGGTG 
CTCAGCACGG GGGCCCAGGA TGGAGGCGTC TACACCTGCA CCGCCCAGAA CCTGGCGGGT 
GAGGTCTCCT GCAAAGCAGA GTTGGCTGTG CATTCAGCTC AGACAGCTAT GGAGGTCGAG 
GGGGTCGGGG AGGATGAGGA CCATCGAGGA AGGAGACTCA GCGACTTTTA TGACATCCAC 
CAGGAGATCG GCAGGGGTGC TTTCTCCTAC TTGCGGCGCA TAGTGGAGCG TAGCTCCGGC 
CTGGAGTTTG CGGCCAAGTT CATCCCCAGC CAGGCCAAGC CAAAGGCATC AGCGCGTCGG 
GAGGCCCGGC TGCTGGCCAG GCTCCAGCAC GACTGTGTCC TCTACTTCCA TGAGGCCTTC 
GAGAGGCGCC GGGGACTGGT CATTGTCACC GAGCTCTGCA CAGAGGAGCT GCTGGAGCGA 
ATCGCCAGGA AACCCACCGT GTGTGAGTCT GAGATCCGGG CCTATATGCG GCAGGTGCTA 
GAGGGAATAC ACTACCTGCA CCAGAGCCAC GTGCTGCACC TCGATGTCAA GCCTGAGAAC 
CTGCTGGTGT GGGATGGTGC TGCGGGCGAG CAGCAGGTGC GGATCTGTGA CTTTGGGAAT 
GCCCAGGAGC TGACTCCAGG AGAGCCCCAG TACTGCCAGT ATGGCACACC TGAGTTTGTA 
GCACCCGAGA TTGTCAATCA GAGCCCCGTG TCTGGAGTCA CTGACATCTG GCCTGTGGGT 
GTTGTTGCCT TCCTCTGTCT GACAGGAATC TCCCCGTTTG TTGGGGAAAA TGACCGGACA 
ACATTGATGA ACATCCGAAA CTACAACGTG GCCTTCGAGG AGACCACATT CCTGAGCCTG 
AGCAGGGAGG CCCGGGGCTT CCTCATCAAA GTGTTGGTGC AGGACCGGCT GAGACCTACC 
GCAGAAGAGA CCCTAGAACA TCCTTGGTTC AAAACTCAGG CAAAGGGCGC AGAGGTGAGC 
ACGGATCACC TGAAGCTATT CCTCTCCCGG CGGAGGTGGC AGCGCTCCCA GATCAGCTAC 
AAATGCCACC TGGTGCTGCG CCCCATCCCC GAGCTGCTGC GGGCCCCCCC AGAGCGGGTG 
TGGGTGACCA TGCCCAGAAG GCCACCCCCC AGTGGGGGGC TCTCATCCTC CTCGGATTCT 
GAAGAGGAAG AGCTGGAAGA GCTGCCCTCA GTGCCCCGCC CACTGCAGCC CGAGTTCTCT 
GGCTCCCGGG TGTCCCTCAC AGACATTCCC ACTGAGGATG AGGCCCTGGG GACCCCAGAG 
ACTGGGGCTG CCACCCCCAT GGACTGGCAG GAGCAGGGAA GGGCTCCCTC TCAGGACCAG 
GAGGCTCCCA GCCCAGAGGC CCTCCCCTCC CCAGGCCAGG AGCCCGCAGC TGGGGCTAGC 
CCCAGGCGGG GAGAGCTCCG CAGGGGCAGC TCGGCTGAGA GCGCCCTGCC CCGGGCCGGG 
CCGCGGGAGC TGGGCCGGGG CCTGCACAAG GCGGCGTCTG TGGAGCTGCC GCAGCGCCGG 
AGCCCCAGCC CGGGAGCCAC CCGCCTGGCC CGGGGAGGCC TGGGTGAGGG CGAGTATGCC 
CAGAGGCTGC AGGCCCTGCG CCAGCGGCTG CTGCGGGGAG GCCCCGAGGA TGGCAAGGTC 
AGCGGCCTCA GGGGTCCCCT GCTGGAGAGC CTGGGGGGCC GTGCTCGGGA CCCCCGGATG 
GCACGAGCTG CCTCCAGCGA GGCAGCGCCC CACCACCAGC CCCCACTCGA GAACCGGGGC 
CTGCAAAAGA GCAGCAGCTT CTCCCAGGGT GAGGCGGAGC CCCGGGGCCG GCACCGCCGA 
GCGGGGGCGC CCCTCGAGAT CCCCGTGGCC AGGCTTGGGG CCCGTAGGCT ACAGGAGTCT 
CCTTCCCTGT CTGCCCTCAG CGAGGCCCAG CCATCCAGCC CTGCACGGCC CAGCGCCCCC 
AAACCCAGTA CCCCTAAGTC TGCAGAACCT TCTGCCACCA CACCTAGTGA TGCTCCGCAG 
CCCCCCGCAC CCCAGCCTGC CCAAGACAAG GCTCCAGAGC CCAGGCCAGA ACCAGTCCGA 
GCCTCCAAGC CTGCACCACC CCCCCAGGCC CTGCAAACCC TAGCGCTGCC CCTCACACCC 
TATGCTCAGA TCATTCAGTC CCTCCAGCTG TCAGGCCACG CCCAGGGCCC CTCGCAGGGC 
CCTGCCGCGC CGCCTTCAGA GCCCAAGCCC CACGCTGCTG TCTTTGCCAG GGTGGCCTCC 
CCACCTCCGG GAGCCCCCGA GAAGCGCGTG CCCTCAGCCG GGGGTCCCCC GGTGCTAGCC 
GAGAAAGCCC GAGTTCCCAC GGTGCCCCCC AGGCCAGGCA GCAGTCTCAG TAGCAGCATC 
GAAAACTTGG AGTCGGAGGC CGTGTTCGAG GCCAAGTTCA AGCGCAGCCG CGAGTCGCCC 
CTGTCGCTGG GGCTGCGGCT GCTGAGCCGT TCGCGCTCGG AGGAGCGCGG CCCCTTCCGT 
GGGGCCGAGG AGGAGGATGG CATATACCGG CCCAGCCCGG CGGGGACCCC GCTGGAGCTG 
GTGCGACGGC CTGAGCGCTC ACGCTCGGTG CAGGACCTCA GGGCTGTCGG AGAGCCTGGC 
CTCGTCCGCC GCCTCTCGCT GTCACTGTCC CAGCGGCTGC GGCGGACCCC TCCCGCGCAG 
CGCCACCCGG CCTGGGAGGC CCGCGGCGGG GACGGAGAGA GCTCGGAGGG CGGGAGCTCG 
GCGCGGGGCT CCCCGGTGCT GGCGATGCGC AGGCGGCTGA GCTTCACCCT GGAGCGGCTG 
TCCAGCCGAT TGCAGCGCAG TGGCAGCAGC GAGGACTCGG GGGGCGCGTC GGGCCGCAGC 
ACGCCGCTGT TCGGACGGCT TCGCAGGGCC ACGTCCGAGG GCGAGAGTCT GCGGCGCCTT 
GGCCTTCCGC ACAACCAGTT GGCCGCCCAG GCCGGCGCCA CCACGCCTTC CGCCGAGTCC 
CTGGGCTCCG AGGCCAGCGC CACGTCGGGC TCCTCAGCCC CAGGGGAAAG CCGAAGCCGG 
CTCCGCTGGG GCTTCTCTCG GCCGCGGAAG GACAAGGGGT TATCGCCACC AAACCTCTCT 
GCCAGCGTCC AGGAGGAGTT GGGTCACCAG TACGTGCGCA GTGAGTCAGA CTTCCCCCCA 
GTCTTCCACA TCAAACTCAA GGACCAGGTG CTGCTGGAGG GGGAGGCAGC CACCCTGCTC 
TGCCTGCCAG CGGCCTGCCC TGCACCGCAC ATCTCCTGGA TGAAAGACAA GAAGTCCTTG 
AGGTCAGAGC CCTCAGTGAT CATCGTGTCC TGCAAAGATG GGCGGCAGCT GCTCAGCATC 
CCCCGGGCGG GCAAGCGGCA CGCCGGTCTC TATGAGTGCT CGGCCACCAA CGTACTGGGC 
AGCATCACCA GCTCCTGTAC CGTGGCTGTG GCCCGAGTCC CAGGAAAGCT AGCTCCTCCA 
GAGGTACCCC AGACCTACCA GGACACGGCG CTGGTGCTGT GGAAGCCGGG AGACAGCCGG 
GCACCTTGCA CGTATACGCT GGAGCGGCGA GTGGATGGGG AGTCTGTGTG GCACCCTGTG 
AGCTCAGGCA TCCCCGACTG TTACTACAAC GTGACCCACC TGCCAGTTGG CGTGACTGTG 
AGGTTCCGTG TGGCCTGTGC CAACCGTGCT GGGCAGGGGC CCTTCAGCAA CTCTTCTGAG 
AAGGTCTTTG TCAGGGGTAC TCAAGATTCT TCAGCTGTGC CATCTGCTGC CCACCAAGAG 
GCCCCTGTCA CCTCAAGGCC AGCCAGGGCC CGGCCTCCTG ACTCTCCTAC CTCACTGGCC 
CCACCCCTAG CTCCTGCTGC CCCCACACCC CCGTCAGTCA CTGTCAGCCC CTCATCTCCC 
CCCACACCTC CTAGCCAGGC CTTGTCCTCG CTCAAGGCTG TGGGTCCACC ACCCCAAACC 
CCTCCACGAA GACACAGGGG CCTGCAGGCT GCCCGGCCAG CGGAGCCCAC CCTACCCAGT 
ACCCACGTCA CCCCAAGTGA GCCCAAGCCT TTCGTCCTTG ACACTGGGAC CCCGATCCCA 
GCCTCCACTC CTCAAGGGGT TAAACCAGTG TCTTCCTCTA CTCCTGTGTA TGTGGTGACT 
TCCTTTGTGT CTGCACCACC AGCCCCTGAG CCCCCAGCCC CTGAGCCCCC TCCTGAGCCT 
ACCAAGGTGA CTGTGCAGAG CCTCAGCCCG GCCAAGGAGG TGGTCAGCTC CCCTGGGAGC 
AGTCCCCGAA GCTCTCCCAG GCCTGAGGGT ACCACTCTTC GACAGGGTCC CCCTCAGAAA 
CCCTACACCT TCCTGGAGGA GAAAGCCAGG GGCCGCTTTG GTGTTGTGCG AGCGTGCCGG 
GAGAATGCCA CGGGGCGAAC GTTCGTGGCC AAGATCGTGC CCTATGCTGC CGAGGGCAAG 
CGGCGGGTCC TGCAGGAGTA CGAGGTGCTG CGGACCCTGC ACCACGAGCG GATCATGTCC 
CTGCACGAGG CCTACATCAC CCCTCGGTAC CTCGTGCTCA TTGCTGAGAG CTGTGGCAAC 
CGGGAACTCC TCTGTGGGCT CAGTGACAGG TTCCGGTATT CTGAGGATGA CGTGGCCACT 
TACATGGTGC AGCTGCTACA AGGCCTGGAC TACCTCCACG GCCACCACGT GCTCCACCTA 
GACATCAAGC CAGACAACCT GCTGCTGGCC CCTGACAATG CCCTCAAGAT TGTGGACTTT 
GGCAGTGCCC AGCCCTACAA CCCCCAGGCC CTTAGGCCCC TTGGCCACCG CACGGGCACG 
CTGGAGTTCA TGGCTCCGGA GATGGTGAAG GGAGAACCCA TCGGCTCTGC CACGGACATC 
TGGGGAGCGG GTGTGCTCAC TTACATTATG CTCAGTGGAC GCTCCCCGTT CTATGAGCCA 
GACCCCCAGG AAACGGAGGC TCGGATTGTG GGGGGCCGCT TTGATGCCTT CCAGCTGTAC 
CCCAATACAT CCCAGAGCGC CACCCTCTTC TTGCGAAAGG TTCTCTCTGT ACATCCCTGG 
TTCATCATCC CTGGCTCTGC CTGGCAGGAG CCGGCCCTCC CTGCAGGACT GCCTGGCCCA 
CCCATGGTTG CAGGACGCCT ACCTGATGAA GCTGCGCCGC CAGACGCTCA CCTTCACCAC 
CAACCGGCTC AAGGAGTTCC TGGGCGAGCA GCGGCGGCGC CGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858646.1
CDS	16..9543
Translation	MSCPLFSAYCNPIPPPILTDEDLEVERGLVSPSNEVFRSCRKQSYDSETAEDDISDVQGTQRLELRDDGAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRRLPGSPRQAQATGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSVPQSGLRREEPDLQPQLASEAPRRPAQPPPSKSALLPPPSPRVGKRSPPGPPAQPAATPTSPHRRTQEPVLPEDTTTEEKRGKKSKSSGPSLAGTAESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSERGAPWGTPGASQEELRAPGSVAERRRLFQQKAASLDERTRQRSPASDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAAAQPPSPSSAEKPGDEPGRPRSRGPAGRTEPGEGPQQEVRRRDQFPLTRSRAIQECRSPVPPPAADPPEARTKAPPGRKREPPAQAVRFLPWATPGLEGAAVPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGARSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNVVVAPGADVLLKCIITANPPPQVSWHKDGSALRSEGRLLLRAEGERHTLLLREARAADAGSYMATATNELGQATCAASLTVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPTDVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARFDCKISGTPPPVVTWTHFGCPMEESENLRLRQDGGLHSLHIAHVGSEDEGLYAVSAVNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEQGELERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDVHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPQVVAVTGRMVTLTWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPGWAATGLRKGVQHIFRVLSTTVKSSSKPSPPSEPVQLLEHGPTLEEAPAMLDKPDIVYVVEGQPASVTVTFNHVEAQVVWRSCRGALLEARAGVYELSQPDDDQYCLRICRVSRRDMGALTCTARNRHGTQTCSVTLELAEAPRFESIMEDVEVGAGETARFAVVVEGKPLPDIMWYKDEVLLTESSHVSFVYEENECSLVVLSTGAQDGGVYTCTAQNLAGEVSCKAELAVHSAQTAMEVEGVGEDEDHRGRRLSDFYDIHQEIGRGAFSYLRRIVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHDCVLYFHEAFERRRGLVIVTELCTEELLERIARKPTVCESEIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTQAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGELRRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQPPAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPSEPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEAKFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAVGEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAESLGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPHISWMKDKKSLRSEPSVIIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARVPGKLAPPEVPQTYQDTALVLWKPGDSRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTHLPVGVTVRFRVACANRAGQGPFSNSSEKVFVRGTQDSSAVPSAAHQEAPVTSRPARARPPDSPTSLAPPLAPAAPTPPSVTVSPSSPPTPPSQALSSLKAVGPPPQTPPRRHRGLQAARPAEPTLPSTHVTPSEPKPFVLDTGTPIPASTPQGVKPVSSSTPVYVVTSFVSAPPAPEPPAPEPPPEPTKVTVQSLSPAKEVVSSPGSSPRSSPRPEGTTLRQGPPQKPYTFLEEKARGRFGVVRACRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERIMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYMVQLLQGLDYLHGHHVLHLDIKPDNLLLAPDNALKIVDFGSAQPYNPQALRPLGHRTGTLEFMAPEMVKGEPIGSATDIWGAGVLTYIMLSGRSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWFIIPGSAWQEPALPAGLPGPPMVAGRLPDEAAPPDAHLHHQPAQGVPGRAAAAPG

Target ORF information:

RefSeq Version	XM_017003157.1
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003157.1

ORF Insert Sequence:

ATGTCCTGCC CACTCTTTTC TGCCTATTGC AACCCTATTC CACCACCCAT TCTTACAGAT 
GAGGACTTGG AGGTTGAGAG AGGTTTGGTG TCACCCAGCA ACGAAGTTTT CCGCTCCTGC 
AGAAAGCAAA GTTACGACTC AGAGACGGCT GAGGATGACA TCAGCGATGT GCAGGGAACC 
CAGCGCCTGG AGCTTCGGGA TGACGGGGCC TTCAGCACCC CCACGGGGGG TTCTGACACC 
CTGGTGGGCA CCTCCCTGGA CACACCCCCG ACCTCCGTGA CAGGCACCTC AGAGGAGCAA 
GTGAGCTGGT GGGGCAGCGG GCAGACGGTC CTGGAGCAGG AAGCGGGCAG TGGGGGTGGC 
ACCCGCCGCC TCCCGGGCAG CCCAAGGCAA GCACAGGCAA CCGGGGCCGG GCCACGGCAC 
CTGGGGGTGG AGCCGCTGGT GCGGGCATCT CGAGCTAATC TGGTGGGCGC AAGCTGGGGG 
TCAGAGGATA GCCTTTCCGT GGCCAGTGAC CTGTACGGCA GCGCATTCAG CCTGTACAGA 
GGACGGGCGC TCTCTATCCA CGTCAGCGTC CCTCAGAGCG GGTTGCGCAG GGAGGAGCCC 
GACCTTCAGC CTCAACTGGC CAGCGAAGCC CCACGCCGCC CTGCCCAGCC GCCTCCTTCC 
AAATCCGCGC TGCTCCCCCC ACCGTCCCCT CGGGTCGGGA AGCGGTCCCC GCCGGGACCC 
CCGGCCCAGC CCGCGGCCAC CCCCACGTCG CCCCACCGTC GCACTCAGGA GCCTGTGCTG 
CCCGAGGACA CCACCACCGA AGAGAAGCGA GGGAAGAAGT CCAAGTCGTC CGGGCCCTCC 
CTGGCGGGCA CCGCGGAATC CCGACCCCAG ACGCCACTGA GCGAGGCCTC AGGCCGCCTG 
TCGGCGTTGG GCCGATCGCC TAGGCTGGTG CGCGCCGGCT CCCGCATCCT GGACAAGCTG 
CAGTTCTTCG AGGAGCGACG GCGCAGCCTG GAGCGCAGCG ACTCGCCGCC GGCGCCCCTG 
CGGCCCTGGG TGCCCCTGCG CAAGGCCCGC TCTCTGGAGC AGCCCAAGTC GGAGCGCGGC 
GCACCGTGGG GCACCCCCGG GGCCTCGCAG GAAGAACTGC GGGCGCCAGG CAGCGTGGCC 
GAGCGGCGCC GCCTGTTCCA GCAGAAAGCG GCCTCGCTGG ACGAGCGCAC GCGTCAGCGC 
AGCCCGGCCT CAGACCTCGA GCTGCGCTTC GCCCAGGAGC TGGGCCGCAT CCGCCGCTCC 
ACGTCGCGGG AGGAGCTGGT GCGCTCGCAC GAGTCCCTGC GCGCCACGCT GCAGCGTGCC 
CCATCCCCTC GAGAGCCCGG CGAGCCCCCG CTCTTCTCTC GGCCCTCCAC CCCCAAGACA 
TCGCGGGCCG TGAGCCCCGC CGCCGCCCAG CCGCCCTCTC CGAGCAGCGC GGAGAAGCCG 
GGGGACGAGC CTGGGAGGCC CAGGAGCCGC GGGCCGGCGG GCAGGACAGA GCCGGGGGAA 
GGCCCGCAGC AGGAGGTTAG GCGTCGGGAC CAATTCCCGC TGACCCGGAG CAGAGCCATC 
CAGGAGTGCA GGAGCCCTGT GCCGCCCCCC GCCGCCGATC CCCCAGAGGC CAGGACGAAA 
GCACCCCCCG GTCGGAAGCG GGAGCCCCCG GCGCAGGCCG TGCGCTTCCT GCCCTGGGCC 
ACGCCGGGCC TGGAGGGCGC TGCTGTACCC CAGACCTTGG AGAAGAACAG GGCGGGGCCT 
GAGGCAGAGA AGAGGCTTCG CAGAGGGCCG GAGGAGGACG GTCCCTGGGG GCCCTGGGAC 
CGCCGAGGGG CCCGCAGCCA GGGCAAAGGT CGCCGGGCCC GGCCCACCTC CCCTGAGCTC 
GAGTCTTCGG ATGACTCCTA CGTGTCCGCT GGAGAAGAGC CCCTAGAGGC CCCTGTGTTT 
GAGATCCCCC TGCAGAATGT GGTGGTGGCA CCAGGGGCAG ATGTGCTGCT CAAGTGTATC 
ATCACTGCCA ACCCCCCGCC CCAAGTGTCC TGGCACAAGG ATGGGTCAGC GCTGCGCAGC 
GAGGGCCGCC TCCTCCTCCG GGCTGAGGGT GAGCGGCACA CCCTGCTGCT CAGGGAGGCC 
AGGGCAGCAG ATGCCGGGAG CTATATGGCC ACCGCCACCA ACGAGCTGGG CCAGGCCACC 
TGTGCCGCCT CACTGACCGT GAGACCCGGT GGGTCTACAT CCCCTTTCAG CAGCCCCATC 
ACCTCCGACG AGGAATACCT GAGCCCCCCA GAGGAGTTCC CAGAGCCTGG GGAGACCTGG 
CCGCGAACCC CCACCATGAA GCCCAGTCCC AGCCAGAACC GCCGTTCTTC TGACACTGGC 
TCCAAGGCAC CCCCCACCTT CAAGGTCTCA CTTATGGACC AGTCAGTAAG AGAAGGCCAA 
GATGTCATCA TGAGCATCCG CGTGCAGGGG GAGCCCAAGC CTGTGGTCTC CTGGCTGAGA 
AACCGCCAGC CCGTGCGCCC AGACCAGCGG CGCTTTGCGG AGGAGGCTGA GGGTGGGCTG 
TGCCGGCTGC GGATCCTGGC TGCAGAGCGT GGCGATGCTG GTTTCTACAC TTGCAAAGCG 
GTCAATGAGT ATGGTGCTCG GCAGTGCGAG GCCCGCTTGG AGGTCCGAGC ACACCCTGAA 
AGCCGGTCCC TGGCCGTGCT GGCCCCCCTG CAGGACGTGG ACGTGGGGGC CGGGGAGATG 
GCGCTGTTTG AGTGCCTGGT GGCGGGGCCC ACTGACGTGG AGGTGGATTG GCTGTGCCGT 
GGCCGCCTGC TGCAGCCTGC ACTGCTCAAA TGCAAGATGC ATTTCGATGG CCGCAAATGC 
AAGCTGCTAC TTACATCTGT ACATGAGGAC GACAGTGGCG TCTACACCTG CAAGCTCAGC 
ACGGCCAAAG ATGAGCTGAC CTGCAGTGCC CGGCTGACCG TGCGGCCCTC GTTGGCACCC 
CTGTTCACAC GGCTGCTGGA AGATGTGGAG GTGTTGGAGG GCCGAGCTGC CCGTTTCGAC 
TGCAAGATCA GTGGCACCCC GCCCCCTGTT GTTACCTGGA CTCATTTTGG CTGCCCCATG 
GAGGAGAGTG AGAACTTGCG GCTGCGGCAG GACGGGGGTC TGCACTCACT GCACATTGCC 
CATGTGGGCA GCGAGGACGA GGGGCTCTAT GCGGTCAGTG CTGTTAACAC CCATGGCCAG 
GCCCACTGCT CAGCCCAGCT GTATGTAGAA GAGCCCCGGA CAGCCGCCTC AGGCCCCAGC 
TCGAAGCTGG AGAAGATGCC ATCCATTCCC GAGGAGCCAG AGCAGGGTGA GCTGGAGCGG 
CTGTCCATTC CTGACTTCCT GCGGCCACTG CAGGACCTGG AGGTGGGACT GGCCAAGGAG 
GCCATGCTAG AGTGCCAGGT GACCGGCCTG CCCTACCCCA CCATCAGCTG GTTCCACAAT 
GGCCACCGCA TCCAGAGCAG CGACGACCGG CGCATGACAC AGTACAGGGA TGTCCATCGC 
TTGGTGTTCC CTGCCGTGGG GCCTCAGCAC GCCGGTGTCT ACAAGAGCGT CATTGCCAAC 
AAGCTGGGCA AAGCTGCCTG CTATGCCCAC CTGTATGTCA CAGATGTGGT CCCAGGCCCT 
CCAGATGGCG CCCCGCAGGT GGTGGCTGTG ACGGGGAGGA TGGTCACACT CACATGGAAC 
CCCCCCAGGA GTCTGGACAT GGCCATCGAC CCGGACTCCC TGACGTACAC AGTGCAGCAC 
CAGGTGCTGG GCTCGGACCA GTGGACGGCA CTGGTCACAG GCCTGCGGGA GCCAGGGTGG 
GCAGCCACAG GGCTGCGTAA GGGGGTCCAG CACATCTTCC GGGTCCTCAG CACCACTGTC 
AAGAGCAGCA GCAAGCCCTC ACCCCCTTCT GAGCCTGTGC AGCTGCTGGA GCACGGCCCA 
ACCCTGGAGG AGGCCCCTGC CATGCTGGAC AAACCAGACA TCGTGTATGT GGTGGAGGGA 
CAGCCTGCCA GCGTCACCGT CACATTCAAC CATGTGGAGG CCCAGGTCGT CTGGAGGAGC 
TGCCGAGGGG CCCTCCTAGA GGCACGGGCC GGTGTGTACG AGCTGAGCCA GCCAGATGAT 
GACCAGTACT GTCTTCGGAT CTGCCGGGTG AGCCGCCGGG ACATGGGGGC CCTCACCTGC 
ACCGCCCGAA ACCGTCACGG CACACAGACC TGCTCGGTCA CATTGGAGCT GGCAGAGGCC 
CCTCGGTTTG AGTCCATCAT GGAGGACGTG GAGGTGGGGG CTGGGGAAAC TGCTCGCTTT 
GCGGTGGTGG TCGAGGGAAA ACCACTGCCG GACATCATGT GGTACAAGGA CGAGGTGCTG 
CTGACCGAGA GCAGCCATGT GAGCTTCGTG TACGAGGAGA ATGAGTGCTC CCTGGTGGTG 
CTCAGCACGG GGGCCCAGGA TGGAGGCGTC TACACCTGCA CCGCCCAGAA CCTGGCGGGT 
GAGGTCTCCT GCAAAGCAGA GTTGGCTGTG CATTCAGCTC AGACAGCTAT GGAGGTCGAG 
GGGGTCGGGG AGGATGAGGA CCATCGAGGA AGGAGACTCA GCGACTTTTA TGACATCCAC 
CAGGAGATCG GCAGGGGTGC TTTCTCCTAC TTGCGGCGCA TAGTGGAGCG TAGCTCCGGC 
CTGGAGTTTG CGGCCAAGTT CATCCCCAGC CAGGCCAAGC CAAAGGCATC AGCGCGTCGG 
GAGGCCCGGC TGCTGGCCAG GCTCCAGCAC GACTGTGTCC TCTACTTCCA TGAGGCCTTC 
GAGAGGCGCC GGGGACTGGT CATTGTCACC GAGCTCTGCA CAGAGGAGCT GCTGGAGCGA 
ATCGCCAGGA AACCCACCGT GTGTGAGTCT GAGATCCGGG CCTATATGCG GCAGGTGCTA 
GAGGGAATAC ACTACCTGCA CCAGAGCCAC GTGCTGCACC TCGATGTCAA GCCTGAGAAC 
CTGCTGGTGT GGGATGGTGC TGCGGGCGAG CAGCAGGTGC GGATCTGTGA CTTTGGGAAT 
GCCCAGGAGC TGACTCCAGG AGAGCCCCAG TACTGCCAGT ATGGCACACC TGAGTTTGTA 
GCACCCGAGA TTGTCAATCA GAGCCCCGTG TCTGGAGTCA CTGACATCTG GCCTGTGGGT 
GTTGTTGCCT TCCTCTGTCT GACAGGAATC TCCCCGTTTG TTGGGGAAAA TGACCGGACA 
ACATTGATGA ACATCCGAAA CTACAACGTG GCCTTCGAGG AGACCACATT CCTGAGCCTG 
AGCAGGGAGG CCCGGGGCTT CCTCATCAAA GTGTTGGTGC AGGACCGGCT GAGACCTACC 
GCAGAAGAGA CCCTAGAACA TCCTTGGTTC AAAACTCAGG CAAAGGGCGC AGAGGTGAGC 
ACGGATCACC TGAAGCTATT CCTCTCCCGG CGGAGGTGGC AGCGCTCCCA GATCAGCTAC 
AAATGCCACC TGGTGCTGCG CCCCATCCCC GAGCTGCTGC GGGCCCCCCC AGAGCGGGTG 
TGGGTGACCA TGCCCAGAAG GCCACCCCCC AGTGGGGGGC TCTCATCCTC CTCGGATTCT 
GAAGAGGAAG AGCTGGAAGA GCTGCCCTCA GTGCCCCGCC CACTGCAGCC CGAGTTCTCT 
GGCTCCCGGG TGTCCCTCAC AGACATTCCC ACTGAGGATG AGGCCCTGGG GACCCCAGAG 
ACTGGGGCTG CCACCCCCAT GGACTGGCAG GAGCAGGGAA GGGCTCCCTC TCAGGACCAG 
GAGGCTCCCA GCCCAGAGGC CCTCCCCTCC CCAGGCCAGG AGCCCGCAGC TGGGGCTAGC 
CCCAGGCGGG GAGAGCTCCG CAGGGGCAGC TCGGCTGAGA GCGCCCTGCC CCGGGCCGGG 
CCGCGGGAGC TGGGCCGGGG CCTGCACAAG GCGGCGTCTG TGGAGCTGCC GCAGCGCCGG 
AGCCCCAGCC CGGGAGCCAC CCGCCTGGCC CGGGGAGGCC TGGGTGAGGG CGAGTATGCC 
CAGAGGCTGC AGGCCCTGCG CCAGCGGCTG CTGCGGGGAG GCCCCGAGGA TGGCAAGGTC 
AGCGGCCTCA GGGGTCCCCT GCTGGAGAGC CTGGGGGGCC GTGCTCGGGA CCCCCGGATG 
GCACGAGCTG CCTCCAGCGA GGCAGCGCCC CACCACCAGC CCCCACTCGA GAACCGGGGC 
CTGCAAAAGA GCAGCAGCTT CTCCCAGGGT GAGGCGGAGC CCCGGGGCCG GCACCGCCGA 
GCGGGGGCGC CCCTCGAGAT CCCCGTGGCC AGGCTTGGGG CCCGTAGGCT ACAGGAGTCT 
CCTTCCCTGT CTGCCCTCAG CGAGGCCCAG CCATCCAGCC CTGCACGGCC CAGCGCCCCC 
AAACCCAGTA CCCCTAAGTC TGCAGAACCT TCTGCCACCA CACCTAGTGA TGCTCCGCAG 
CCCCCCGCAC CCCAGCCTGC CCAAGACAAG GCTCCAGAGC CCAGGCCAGA ACCAGTCCGA 
GCCTCCAAGC CTGCACCACC CCCCCAGGCC CTGCAAACCC TAGCGCTGCC CCTCACACCC 
TATGCTCAGA TCATTCAGTC CCTCCAGCTG TCAGGCCACG CCCAGGGCCC CTCGCAGGGC 
CCTGCCGCGC CGCCTTCAGA GCCCAAGCCC CACGCTGCTG TCTTTGCCAG GGTGGCCTCC 
CCACCTCCGG GAGCCCCCGA GAAGCGCGTG CCCTCAGCCG GGGGTCCCCC GGTGCTAGCC 
GAGAAAGCCC GAGTTCCCAC GGTGCCCCCC AGGCCAGGCA GCAGTCTCAG TAGCAGCATC 
GAAAACTTGG AGTCGGAGGC CGTGTTCGAG GCCAAGTTCA AGCGCAGCCG CGAGTCGCCC 
CTGTCGCTGG GGCTGCGGCT GCTGAGCCGT TCGCGCTCGG AGGAGCGCGG CCCCTTCCGT 
GGGGCCGAGG AGGAGGATGG CATATACCGG CCCAGCCCGG CGGGGACCCC GCTGGAGCTG 
GTGCGACGGC CTGAGCGCTC ACGCTCGGTG CAGGACCTCA GGGCTGTCGG AGAGCCTGGC 
CTCGTCCGCC GCCTCTCGCT GTCACTGTCC CAGCGGCTGC GGCGGACCCC TCCCGCGCAG 
CGCCACCCGG CCTGGGAGGC CCGCGGCGGG GACGGAGAGA GCTCGGAGGG CGGGAGCTCG 
GCGCGGGGCT CCCCGGTGCT GGCGATGCGC AGGCGGCTGA GCTTCACCCT GGAGCGGCTG 
TCCAGCCGAT TGCAGCGCAG TGGCAGCAGC GAGGACTCGG GGGGCGCGTC GGGCCGCAGC 
ACGCCGCTGT TCGGACGGCT TCGCAGGGCC ACGTCCGAGG GCGAGAGTCT GCGGCGCCTT 
GGCCTTCCGC ACAACCAGTT GGCCGCCCAG GCCGGCGCCA CCACGCCTTC CGCCGAGTCC 
CTGGGCTCCG AGGCCAGCGC CACGTCGGGC TCCTCAGCCC CAGGGGAAAG CCGAAGCCGG 
CTCCGCTGGG GCTTCTCTCG GCCGCGGAAG GACAAGGGGT TATCGCCACC AAACCTCTCT 
GCCAGCGTCC AGGAGGAGTT GGGTCACCAG TACGTGCGCA GTGAGTCAGA CTTCCCCCCA 
GTCTTCCACA TCAAACTCAA GGACCAGGTG CTGCTGGAGG GGGAGGCAGC CACCCTGCTC 
TGCCTGCCAG CGGCCTGCCC TGCACCGCAC ATCTCCTGGA TGAAAGACAA GAAGTCCTTG 
AGGTCAGAGC CCTCAGTGAT CATCGTGTCC TGCAAAGATG GGCGGCAGCT GCTCAGCATC 
CCCCGGGCGG GCAAGCGGCA CGCCGGTCTC TATGAGTGCT CGGCCACCAA CGTACTGGGC 
AGCATCACCA GCTCCTGTAC CGTGGCTGTG GCCCGAGTCC CAGGAAAGCT AGCTCCTCCA 
GAGGTACCCC AGACCTACCA GGACACGGCG CTGGTGCTGT GGAAGCCGGG AGACAGCCGG 
GCACCTTGCA CGTATACGCT GGAGCGGCGA GTGGATGGGG AGTCTGTGTG GCACCCTGTG 
AGCTCAGGCA TCCCCGACTG TTACTACAAC GTGACCCACC TGCCAGTTGG CGTGACTGTG 
AGGTTCCGTG TGGCCTGTGC CAACCGTGCT GGGCAGGGGC CCTTCAGCAA CTCTTCTGAG 
AAGGTCTTTG TCAGGGGTAC TCAAGATTCT TCAGCTGTGC CATCTGCTGC CCACCAAGAG 
GCCCCTGTCA CCTCAAGGCC AGCCAGGGCC CGGCCTCCTG ACTCTCCTAC CTCACTGGCC 
CCACCCCTAG CTCCTGCTGC CCCCACACCC CCGTCAGTCA CTGTCAGCCC CTCATCTCCC 
CCCACACCTC CTAGCCAGGC CTTGTCCTCG CTCAAGGCTG TGGGTCCACC ACCCCAAACC 
CCTCCACGAA GACACAGGGG CCTGCAGGCT GCCCGGCCAG CGGAGCCCAC CCTACCCAGT 
ACCCACGTCA CCCCAAGTGA GCCCAAGCCT TTCGTCCTTG ACACTGGGAC CCCGATCCCA 
GCCTCCACTC CTCAAGGGGT TAAACCAGTG TCTTCCTCTA CTCCTGTGTA TGTGGTGACT 
TCCTTTGTGT CTGCACCACC AGCCCCTGAG CCCCCAGCCC CTGAGCCCCC TCCTGAGCCT 
ACCAAGGTGA CTGTGCAGAG CCTCAGCCCG GCCAAGGAGG TGGTCAGCTC CCCTGGGAGC 
AGTCCCCGAA GCTCTCCCAG GCCTGAGGGT ACCACTCTTC GACAGGGTCC CCCTCAGAAA 
CCCTACACCT TCCTGGAGGA GAAAGCCAGG GGCCGCTTTG GTGTTGTGCG AGCGTGCCGG 
GAGAATGCCA CGGGGCGAAC GTTCGTGGCC AAGATCGTGC CCTATGCTGC CGAGGGCAAG 
CGGCGGGTCC TGCAGGAGTA CGAGGTGCTG CGGACCCTGC ACCACGAGCG GATCATGTCC 
CTGCACGAGG CCTACATCAC CCCTCGGTAC CTCGTGCTCA TTGCTGAGAG CTGTGGCAAC 
CGGGAACTCC TCTGTGGGCT CAGTGACAGG TTCCGGTATT CTGAGGATGA CGTGGCCACT 
TACATGGTGC AGCTGCTACA AGGCCTGGAC TACCTCCACG GCCACCACGT GCTCCACCTA 
GACATCAAGC CAGACAACCT GCTGCTGGCC CCTGACAATG CCCTCAAGAT TGTGGACTTT 
GGCAGTGCCC AGCCCTACAA CCCCCAGGCC CTTAGGCCCC TTGGCCACCG CACGGGCACG 
CTGGAGTTCA TGGCTCCGGA GATGGTGAAG GGAGAACCCA TCGGCTCTGC CACGGACATC 
TGGGGAGCGG GTGTGCTCAC TTACATTATG CTCAGTGGAC GCTCCCCGTT CTATGAGCCA 
GACCCCCAGG AAACGGAGGC TCGGATTGTG GGGGGCCGCT TTGATGCCTT CCAGCTGTAC 
CCCAATACAT CCCAGAGCGC CACCCTCTTC TTGCGAAAGG TTCTCTCTGT ACATCCCTGG 
TTCATCATCC CTGGCTCTGC CTGGCAGGAG CCGGCCCTCC CTGCAGGACT GCCTGGCCCA 
CCCATGGTTG CAGGACGCCT ACCTGATGAA GCTGCGCCGC CAGACGCTCA CCTTCACCAC 
CAACCGGCTC AAGGAGTTCC TGGGCGAGCA GCGGCGGCGC CGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_017003159.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858648.1 Homo sapiens SPEG complex locus (SPEG), transcript variant X8, mRNA.

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CloneID	OHu88920
Clone ID Related Accession (Same CDS sequence)	XM_017003159.1 , XM_017003159.2
Accession Version	XM_017003159.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCG CCACCAACGA GCTGGGCCAG GCCACCTGTG CCGCCTCACT GACCGTGAGA 
CCCGGTGGGT CTACATCCCC TTTCAGCAGC CCCATCACCT CCGACGAGGA ATACCTGAGC 
CCCCCAGAGG AGTTCCCAGA GCCTGGGGAG ACCTGGCCGC GAACCCCCAC CATGAAGCCC 
AGTCCCAGCC AGAACCGCCG TTCTTCTGAC ACTGGCTCCA AGGCACCCCC CACCTTCAAG 
GTCTCACTTA TGGACCAGTC AGTAAGAGAA GGCCAAGATG TCATCATGAG CATCCGCGTG 
CAGGGGGAGC CCAAGCCTGT GGTCTCCTGG CTGAGAAACC GCCAGCCCGT GCGCCCAGAC 
CAGCGGCGCT TTGCGGAGGA GGCTGAGGGT GGGCTGTGCC GGCTGCGGAT CCTGGCTGCA 
GAGCGTGGCG ATGCTGGTTT CTACACTTGC AAAGCGGTCA ATGAGTATGG TGCTCGGCAG 
TGCGAGGCCC GCTTGGAGGT CCGAGCACAC CCTGAAAGCC GGTCCCTGGC CGTGCTGGCC 
CCCCTGCAGG ACGTGGACGT GGGGGCCGGG GAGATGGCGC TGTTTGAGTG CCTGGTGGCG 
GGGCCCACTG ACGTGGAGGT GGATTGGCTG TGCCGTGGCC GCCTGCTGCA GCCTGCACTG 
CTCAAATGCA AGATGCATTT CGATGGCCGC AAATGCAAGC TGCTACTTAC ATCTGTACAT 
GAGGACGACA GTGGCGTCTA CACCTGCAAG CTCAGCACGG CCAAAGATGA GCTGACCTGC 
AGTGCCCGGC TGACCGTGCG GCCCTCGTTG GCACCCCTGT TCACACGGCT GCTGGAAGAT 
GTGGAGGTGT TGGAGGGCCG AGCTGCCCGT TTCGACTGCA AGATCAGTGG CACCCCGCCC 
CCTGTTGTTA CCTGGACTCA TTTTGGCTGC CCCATGGAGG AGAGTGAGAA CTTGCGGCTG 
CGGCAGGACG GGGGTCTGCA CTCACTGCAC ATTGCCCATG TGGGCAGCGA GGACGAGGGG 
CTCTATGCGG TCAGTGCTGT TAACACCCAT GGCCAGGCCC ACTGCTCAGC CCAGCTGTAT 
GTAGAAGAGC CCCGGACAGC CGCCTCAGGC CCCAGCTCGA AGCTGGAGAA GATGCCATCC 
ATTCCCGAGG AGCCAGAGCA GGGTGAGCTG GAGCGGCTGT CCATTCCTGA CTTCCTGCGG 
CCACTGCAGG ACCTGGAGGT GGGACTGGCC AAGGAGGCCA TGCTAGAGTG CCAGGTGACC 
GGCCTGCCCT ACCCCACCAT CAGCTGGTTC CACAATGGCC ACCGCATCCA GAGCAGCGAC 
GACCGGCGCA TGACACAGTA CAGGGATGTC CATCGCTTGG TGTTCCCTGC CGTGGGGCCT 
CAGCACGCCG GTGTCTACAA GAGCGTCATT GCCAACAAGC TGGGCAAAGC TGCCTGCTAT 
GCCCACCTGT ATGTCACAGA TGTGGTCCCA GGCCCTCCAG ATGGCGCCCC GCAGGTGGTG 
GCTGTGACGG GGAGGATGGT CACACTCACA TGGAACCCCC CCAGGAGTCT GGACATGGCC 
ATCGACCCGG ACTCCCTGAC GTACACAGTG CAGCACCAGG TGCTGGGCTC GGACCAGTGG 
ACGGCACTGG TCACAGGCCT GCGGGAGCCA GGGTGGGCAG CCACAGGGCT GCGTAAGGGG 
GTCCAGCACA TCTTCCGGGT CCTCAGCACC ACTGTCAAGA GCAGCAGCAA GCCCTCACCC 
CCTTCTGAGC CTGTGCAGCT GCTGGAGCAC GGCCCAACCC TGGAGGAGGC CCCTGCCATG 
CTGGACAAAC CAGACATCGT GTATGTGGTG GAGGGACAGC CTGCCAGCGT CACCGTCACA 
TTCAACCATG TGGAGGCCCA GGTCGTCTGG AGGAGCTGCC GAGGGGCCCT CCTAGAGGCA 
CGGGCCGGTG TGTACGAGCT GAGCCAGCCA GATGATGACC AGTACTGTCT TCGGATCTGC 
CGGGTGAGCC GCCGGGACAT GGGGGCCCTC ACCTGCACCG CCCGAAACCG TCACGGCACA 
CAGACCTGCT CGGTCACATT GGAGCTGGCA GAGGCCCCTC GGTTTGAGTC CATCATGGAG 
GACGTGGAGG TGGGGGCTGG GGAAACTGCT CGCTTTGCGG TGGTGGTCGA GGGAAAACCA 
CTGCCGGACA TCATGTGGTA CAAGGACGAG GTGCTGCTGA CCGAGAGCAG CCATGTGAGC 
TTCGTGTACG AGGAGAATGA GTGCTCCCTG GTGGTGCTCA GCACGGGGGC CCAGGATGGA 
GGCGTCTACA CCTGCACCGC CCAGAACCTG GCGGGTGAGG TCTCCTGCAA AGCAGAGTTG 
GCTGTGCATT CAGCTCAGAC AGCTATGGAG GTCGAGGGGG TCGGGGAGGA TGAGGACCAT 
CGAGGAAGGA GACTCAGCGA CTTTTATGAC ATCCACCAGG AGATCGGCAG GGGTGCTTTC 
TCCTACTTGC GGCGCATAGT GGAGCGTAGC TCCGGCCTGG AGTTTGCGGC CAAGTTCATC 
CCCAGCCAGG CCAAGCCAAA GGCATCAGCG CGTCGGGAGG CCCGGCTGCT GGCCAGGCTC 
CAGCACGACT GTGTCCTCTA CTTCCATGAG GCCTTCGAGA GGCGCCGGGG ACTGGTCATT 
GTCACCGAGC TCTGCACAGA GGAGCTGCTG GAGCGAATCG CCAGGAAACC CACCGTGTGT 
GAGTCTGAGA TCCGGGCCTA TATGCGGCAG GTGCTAGAGG GAATACACTA CCTGCACCAG 
AGCCACGTGC TGCACCTCGA TGTCAAGCCT GAGAACCTGC TGGTGTGGGA TGGTGCTGCG 
GGCGAGCAGC AGGTGCGGAT CTGTGACTTT GGGAATGCCC AGGAGCTGAC TCCAGGAGAG 
CCCCAGTACT GCCAGTATGG CACACCTGAG TTTGTAGCAC CCGAGATTGT CAATCAGAGC 
CCCGTGTCTG GAGTCACTGA CATCTGGCCT GTGGGTGTTG TTGCCTTCCT CTGTCTGACA 
GGAATCTCCC CGTTTGTTGG GGAAAATGAC CGGACAACAT TGATGAACAT CCGAAACTAC 
AACGTGGCCT TCGAGGAGAC CACATTCCTG AGCCTGAGCA GGGAGGCCCG GGGCTTCCTC 
ATCAAAGTGT TGGTGCAGGA CCGGCTGAGA CCTACCGCAG AAGAGACCCT AGAACATCCT 
TGGTTCAAAA CTCAGGCAAA GGGCGCAGAG GTGAGCACGG ATCACCTGAA GCTATTCCTC 
TCCCGGCGGA GGTGGCAGCG CTCCCAGATC AGCTACAAAT GCCACCTGGT GCTGCGCCCC 
ATCCCCGAGC TGCTGCGGGC CCCCCCAGAG CGGGTGTGGG TGACCATGCC CAGAAGGCCA 
CCCCCCAGTG GGGGGCTCTC ATCCTCCTCG GATTCTGAAG AGGAAGAGCT GGAAGAGCTG 
CCCTCAGTGC CCCGCCCACT GCAGCCCGAG TTCTCTGGCT CCCGGGTGTC CCTCACAGAC 
ATTCCCACTG AGGATGAGGC CCTGGGGACC CCAGAGACTG GGGCTGCCAC CCCCATGGAC 
TGGCAGGAGC AGGGAAGGGC TCCCTCTCAG GACCAGGAGG CTCCCAGCCC AGAGGCCCTC 
CCCTCCCCAG GCCAGGAGCC CGCAGCTGGG GCTAGCCCCA GGCGGGGAGA GCTCCGCAGG 
GGCAGCTCGG CTGAGAGCGC CCTGCCCCGG GCCGGGCCGC GGGAGCTGGG CCGGGGCCTG 
CACAAGGCGG CGTCTGTGGA GCTGCCGCAG CGCCGGAGCC CCAGCCCGGG AGCCACCCGC 
CTGGCCCGGG GAGGCCTGGG TGAGGGCGAG TATGCCCAGA GGCTGCAGGC CCTGCGCCAG 
CGGCTGCTGC GGGGAGGCCC CGAGGATGGC AAGGTCAGCG GCCTCAGGGG TCCCCTGCTG 
GAGAGCCTGG GGGGCCGTGC TCGGGACCCC CGGATGGCAC GAGCTGCCTC CAGCGAGGCA 
GCGCCCCACC ACCAGCCCCC ACTCGAGAAC CGGGGCCTGC AAAAGAGCAG CAGCTTCTCC 
CAGGGTGAGG CGGAGCCCCG GGGCCGGCAC CGCCGAGCGG GGGCGCCCCT CGAGATCCCC 
GTGGCCAGGC TTGGGGCCCG TAGGCTACAG GAGTCTCCTT CCCTGTCTGC CCTCAGCGAG 
GCCCAGCCAT CCAGCCCTGC ACGGCCCAGC GCCCCCAAAC CCAGTACCCC TAAGTCTGCA 
GAACCTTCTG CCACCACACC TAGTGATGCT CCGCAGCCCC CCGCACCCCA GCCTGCCCAA 
GACAAGGCTC CAGAGCCCAG GCCAGAACCA GTCCGAGCCT CCAAGCCTGC ACCACCCCCC 
CAGGCCCTGC AAACCCTAGC GCTGCCCCTC ACACCCTATG CTCAGATCAT TCAGTCCCTC 
CAGCTGTCAG GCCACGCCCA GGGCCCCTCG CAGGGCCCTG CCGCGCCGCC TTCAGAGCCC 
AAGCCCCACG CTGCTGTCTT TGCCAGGGTG GCCTCCCCAC CTCCGGGAGC CCCCGAGAAG 
CGCGTGCCCT CAGCCGGGGG TCCCCCGGTG CTAGCCGAGA AAGCCCGAGT TCCCACGGTG 
CCCCCCAGGC CAGGCAGCAG TCTCAGTAGC AGCATCGAAA ACTTGGAGTC GGAGGCCGTG 
TTCGAGGCCA AGTTCAAGCG CAGCCGCGAG TCGCCCCTGT CGCTGGGGCT GCGGCTGCTG 
AGCCGTTCGC GCTCGGAGGA GCGCGGCCCC TTCCGTGGGG CCGAGGAGGA GGATGGCATA 
TACCGGCCCA GCCCGGCGGG GACCCCGCTG GAGCTGGTGC GACGGCCTGA GCGCTCACGC 
TCGGTGCAGG ACCTCAGGGC TGTCGGAGAG CCTGGCCTCG TCCGCCGCCT CTCGCTGTCA 
CTGTCCCAGC GGCTGCGGCG GACCCCTCCC GCGCAGCGCC ACCCGGCCTG GGAGGCCCGC 
GGCGGGGACG GAGAGAGCTC GGAGGGCGGG AGCTCGGCGC GGGGCTCCCC GGTGCTGGCG 
ATGCGCAGGC GGCTGAGCTT CACCCTGGAG CGGCTGTCCA GCCGATTGCA GCGCAGTGGC 
AGCAGCGAGG ACTCGGGGGG CGCGTCGGGC CGCAGCACGC CGCTGTTCGG ACGGCTTCGC 
AGGGCCACGT CCGAGGGCGA GAGTCTGCGG CGCCTTGGCC TTCCGCACAA CCAGTTGGCC 
GCCCAGGCCG GCGCCACCAC GCCTTCCGCC GAGTCCCTGG GCTCCGAGGC CAGCGCCACG 
TCGGGCTCCT CAGCCCCAGG GGAAAGCCGA AGCCGGCTCC GCTGGGGCTT CTCTCGGCCG 
CGGAAGGACA AGGGGTTATC GCCACCAAAC CTCTCTGCCA GCGTCCAGGA GGAGTTGGGT 
CACCAGTACG TGCGCAGTGA GTCAGTACCC TCACTTGGCC TGGAGGCAGC CATTGAGAAA 
CTGTGTTCAC ATTGCCTTGT TGGAACCTCA GTGTGGGACC CCTCCTTGGG GAGCAGTGGG 
GTACAGCGGG AAGGGGGCAC ACTGCCATCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858648.1
CDS	1899..7391
Translation	MATATNELGQATCAASLTVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPTDVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARFDCKISGTPPPVVTWTHFGCPMEESENLRLRQDGGLHSLHIAHVGSEDEGLYAVSAVNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEQGELERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDVHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPQVVAVTGRMVTLTWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPGWAATGLRKGVQHIFRVLSTTVKSSSKPSPPSEPVQLLEHGPTLEEAPAMLDKPDIVYVVEGQPASVTVTFNHVEAQVVWRSCRGALLEARAGVYELSQPDDDQYCLRICRVSRRDMGALTCTARNRHGTQTCSVTLELAEAPRFESIMEDVEVGAGETARFAVVVEGKPLPDIMWYKDEVLLTESSHVSFVYEENECSLVVLSTGAQDGGVYTCTAQNLAGEVSCKAELAVHSAQTAMEVEGVGEDEDHRGRRLSDFYDIHQEIGRGAFSYLRRIVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHDCVLYFHEAFERRRGLVIVTELCTEELLERIARKPTVCESEIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTQAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGELRRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQPPAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPSEPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEAKFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAVGEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAESLGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESVPSLGLEAAIEKLCSHCLVGTSVWDPSLGSSGVQREGGTLPS

Target ORF information:

RefSeq Version	XM_017003159.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SPEG complex locus (SPEG), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003159.1

ORF Insert Sequence:

ATGGCCACCG CCACCAACGA GCTGGGCCAG GCCACCTGTG CCGCCTCACT GACCGTGAGA 
CCCGGTGGGT CTACATCCCC TTTCAGCAGC CCCATCACCT CCGACGAGGA ATACCTGAGC 
CCCCCAGAGG AGTTCCCAGA GCCTGGGGAG ACCTGGCCGC GAACCCCCAC CATGAAGCCC 
AGTCCCAGCC AGAACCGCCG TTCTTCTGAC ACTGGCTCCA AGGCACCCCC CACCTTCAAG 
GTCTCACTTA TGGACCAGTC AGTAAGAGAA GGCCAAGATG TCATCATGAG CATCCGCGTG 
CAGGGGGAGC CCAAGCCTGT GGTCTCCTGG CTGAGAAACC GCCAGCCCGT GCGCCCAGAC 
CAGCGGCGCT TTGCGGAGGA GGCTGAGGGT GGGCTGTGCC GGCTGCGGAT CCTGGCTGCA 
GAGCGTGGCG ATGCTGGTTT CTACACTTGC AAAGCGGTCA ATGAGTATGG TGCTCGGCAG 
TGCGAGGCCC GCTTGGAGGT CCGAGCACAC CCTGAAAGCC GGTCCCTGGC CGTGCTGGCC 
CCCCTGCAGG ACGTGGACGT GGGGGCCGGG GAGATGGCGC TGTTTGAGTG CCTGGTGGCG 
GGGCCCACTG ACGTGGAGGT GGATTGGCTG TGCCGTGGCC GCCTGCTGCA GCCTGCACTG 
CTCAAATGCA AGATGCATTT CGATGGCCGC AAATGCAAGC TGCTACTTAC ATCTGTACAT 
GAGGACGACA GTGGCGTCTA CACCTGCAAG CTCAGCACGG CCAAAGATGA GCTGACCTGC 
AGTGCCCGGC TGACCGTGCG GCCCTCGTTG GCACCCCTGT TCACACGGCT GCTGGAAGAT 
GTGGAGGTGT TGGAGGGCCG AGCTGCCCGT TTCGACTGCA AGATCAGTGG CACCCCGCCC 
CCTGTTGTTA CCTGGACTCA TTTTGGCTGC CCCATGGAGG AGAGTGAGAA CTTGCGGCTG 
CGGCAGGACG GGGGTCTGCA CTCACTGCAC ATTGCCCATG TGGGCAGCGA GGACGAGGGG 
CTCTATGCGG TCAGTGCTGT TAACACCCAT GGCCAGGCCC ACTGCTCAGC CCAGCTGTAT 
GTAGAAGAGC CCCGGACAGC CGCCTCAGGC CCCAGCTCGA AGCTGGAGAA GATGCCATCC 
ATTCCCGAGG AGCCAGAGCA GGGTGAGCTG GAGCGGCTGT CCATTCCTGA CTTCCTGCGG 
CCACTGCAGG ACCTGGAGGT GGGACTGGCC AAGGAGGCCA TGCTAGAGTG CCAGGTGACC 
GGCCTGCCCT ACCCCACCAT CAGCTGGTTC CACAATGGCC ACCGCATCCA GAGCAGCGAC 
GACCGGCGCA TGACACAGTA CAGGGATGTC CATCGCTTGG TGTTCCCTGC CGTGGGGCCT 
CAGCACGCCG GTGTCTACAA GAGCGTCATT GCCAACAAGC TGGGCAAAGC TGCCTGCTAT 
GCCCACCTGT ATGTCACAGA TGTGGTCCCA GGCCCTCCAG ATGGCGCCCC GCAGGTGGTG 
GCTGTGACGG GGAGGATGGT CACACTCACA TGGAACCCCC CCAGGAGTCT GGACATGGCC 
ATCGACCCGG ACTCCCTGAC GTACACAGTG CAGCACCAGG TGCTGGGCTC GGACCAGTGG 
ACGGCACTGG TCACAGGCCT GCGGGAGCCA GGGTGGGCAG CCACAGGGCT GCGTAAGGGG 
GTCCAGCACA TCTTCCGGGT CCTCAGCACC ACTGTCAAGA GCAGCAGCAA GCCCTCACCC 
CCTTCTGAGC CTGTGCAGCT GCTGGAGCAC GGCCCAACCC TGGAGGAGGC CCCTGCCATG 
CTGGACAAAC CAGACATCGT GTATGTGGTG GAGGGACAGC CTGCCAGCGT CACCGTCACA 
TTCAACCATG TGGAGGCCCA GGTCGTCTGG AGGAGCTGCC GAGGGGCCCT CCTAGAGGCA 
CGGGCCGGTG TGTACGAGCT GAGCCAGCCA GATGATGACC AGTACTGTCT TCGGATCTGC 
CGGGTGAGCC GCCGGGACAT GGGGGCCCTC ACCTGCACCG CCCGAAACCG TCACGGCACA 
CAGACCTGCT CGGTCACATT GGAGCTGGCA GAGGCCCCTC GGTTTGAGTC CATCATGGAG 
GACGTGGAGG TGGGGGCTGG GGAAACTGCT CGCTTTGCGG TGGTGGTCGA GGGAAAACCA 
CTGCCGGACA TCATGTGGTA CAAGGACGAG GTGCTGCTGA CCGAGAGCAG CCATGTGAGC 
TTCGTGTACG AGGAGAATGA GTGCTCCCTG GTGGTGCTCA GCACGGGGGC CCAGGATGGA 
GGCGTCTACA CCTGCACCGC CCAGAACCTG GCGGGTGAGG TCTCCTGCAA AGCAGAGTTG 
GCTGTGCATT CAGCTCAGAC AGCTATGGAG GTCGAGGGGG TCGGGGAGGA TGAGGACCAT 
CGAGGAAGGA GACTCAGCGA CTTTTATGAC ATCCACCAGG AGATCGGCAG GGGTGCTTTC 
TCCTACTTGC GGCGCATAGT GGAGCGTAGC TCCGGCCTGG AGTTTGCGGC CAAGTTCATC 
CCCAGCCAGG CCAAGCCAAA GGCATCAGCG CGTCGGGAGG CCCGGCTGCT GGCCAGGCTC 
CAGCACGACT GTGTCCTCTA CTTCCATGAG GCCTTCGAGA GGCGCCGGGG ACTGGTCATT 
GTCACCGAGC TCTGCACAGA GGAGCTGCTG GAGCGAATCG CCAGGAAACC CACCGTGTGT 
GAGTCTGAGA TCCGGGCCTA TATGCGGCAG GTGCTAGAGG GAATACACTA CCTGCACCAG 
AGCCACGTGC TGCACCTCGA TGTCAAGCCT GAGAACCTGC TGGTGTGGGA TGGTGCTGCG 
GGCGAGCAGC AGGTGCGGAT CTGTGACTTT GGGAATGCCC AGGAGCTGAC TCCAGGAGAG 
CCCCAGTACT GCCAGTATGG CACACCTGAG TTTGTAGCAC CCGAGATTGT CAATCAGAGC 
CCCGTGTCTG GAGTCACTGA CATCTGGCCT GTGGGTGTTG TTGCCTTCCT CTGTCTGACA 
GGAATCTCCC CGTTTGTTGG GGAAAATGAC CGGACAACAT TGATGAACAT CCGAAACTAC 
AACGTGGCCT TCGAGGAGAC CACATTCCTG AGCCTGAGCA GGGAGGCCCG GGGCTTCCTC 
ATCAAAGTGT TGGTGCAGGA CCGGCTGAGA CCTACCGCAG AAGAGACCCT AGAACATCCT 
TGGTTCAAAA CTCAGGCAAA GGGCGCAGAG GTGAGCACGG ATCACCTGAA GCTATTCCTC 
TCCCGGCGGA GGTGGCAGCG CTCCCAGATC AGCTACAAAT GCCACCTGGT GCTGCGCCCC 
ATCCCCGAGC TGCTGCGGGC CCCCCCAGAG CGGGTGTGGG TGACCATGCC CAGAAGGCCA 
CCCCCCAGTG GGGGGCTCTC ATCCTCCTCG GATTCTGAAG AGGAAGAGCT GGAAGAGCTG 
CCCTCAGTGC CCCGCCCACT GCAGCCCGAG TTCTCTGGCT CCCGGGTGTC CCTCACAGAC 
ATTCCCACTG AGGATGAGGC CCTGGGGACC CCAGAGACTG GGGCTGCCAC CCCCATGGAC 
TGGCAGGAGC AGGGAAGGGC TCCCTCTCAG GACCAGGAGG CTCCCAGCCC AGAGGCCCTC 
CCCTCCCCAG GCCAGGAGCC CGCAGCTGGG GCTAGCCCCA GGCGGGGAGA GCTCCGCAGG 
GGCAGCTCGG CTGAGAGCGC CCTGCCCCGG GCCGGGCCGC GGGAGCTGGG CCGGGGCCTG 
CACAAGGCGG CGTCTGTGGA GCTGCCGCAG CGCCGGAGCC CCAGCCCGGG AGCCACCCGC 
CTGGCCCGGG GAGGCCTGGG TGAGGGCGAG TATGCCCAGA GGCTGCAGGC CCTGCGCCAG 
CGGCTGCTGC GGGGAGGCCC CGAGGATGGC AAGGTCAGCG GCCTCAGGGG TCCCCTGCTG 
GAGAGCCTGG GGGGCCGTGC TCGGGACCCC CGGATGGCAC GAGCTGCCTC CAGCGAGGCA 
GCGCCCCACC ACCAGCCCCC ACTCGAGAAC CGGGGCCTGC AAAAGAGCAG CAGCTTCTCC 
CAGGGTGAGG CGGAGCCCCG GGGCCGGCAC CGCCGAGCGG GGGCGCCCCT CGAGATCCCC 
GTGGCCAGGC TTGGGGCCCG TAGGCTACAG GAGTCTCCTT CCCTGTCTGC CCTCAGCGAG 
GCCCAGCCAT CCAGCCCTGC ACGGCCCAGC GCCCCCAAAC CCAGTACCCC TAAGTCTGCA 
GAACCTTCTG CCACCACACC TAGTGATGCT CCGCAGCCCC CCGCACCCCA GCCTGCCCAA 
GACAAGGCTC CAGAGCCCAG GCCAGAACCA GTCCGAGCCT CCAAGCCTGC ACCACCCCCC 
CAGGCCCTGC AAACCCTAGC GCTGCCCCTC ACACCCTATG CTCAGATCAT TCAGTCCCTC 
CAGCTGTCAG GCCACGCCCA GGGCCCCTCG CAGGGCCCTG CCGCGCCGCC TTCAGAGCCC 
AAGCCCCACG CTGCTGTCTT TGCCAGGGTG GCCTCCCCAC CTCCGGGAGC CCCCGAGAAG 
CGCGTGCCCT CAGCCGGGGG TCCCCCGGTG CTAGCCGAGA AAGCCCGAGT TCCCACGGTG 
CCCCCCAGGC CAGGCAGCAG TCTCAGTAGC AGCATCGAAA ACTTGGAGTC GGAGGCCGTG 
TTCGAGGCCA AGTTCAAGCG CAGCCGCGAG TCGCCCCTGT CGCTGGGGCT GCGGCTGCTG 
AGCCGTTCGC GCTCGGAGGA GCGCGGCCCC TTCCGTGGGG CCGAGGAGGA GGATGGCATA 
TACCGGCCCA GCCCGGCGGG GACCCCGCTG GAGCTGGTGC GACGGCCTGA GCGCTCACGC 
TCGGTGCAGG ACCTCAGGGC TGTCGGAGAG CCTGGCCTCG TCCGCCGCCT CTCGCTGTCA 
CTGTCCCAGC GGCTGCGGCG GACCCCTCCC GCGCAGCGCC ACCCGGCCTG GGAGGCCCGC 
GGCGGGGACG GAGAGAGCTC GGAGGGCGGG AGCTCGGCGC GGGGCTCCCC GGTGCTGGCG 
ATGCGCAGGC GGCTGAGCTT CACCCTGGAG CGGCTGTCCA GCCGATTGCA GCGCAGTGGC 
AGCAGCGAGG ACTCGGGGGG CGCGTCGGGC CGCAGCACGC CGCTGTTCGG ACGGCTTCGC 
AGGGCCACGT CCGAGGGCGA GAGTCTGCGG CGCCTTGGCC TTCCGCACAA CCAGTTGGCC 
GCCCAGGCCG GCGCCACCAC GCCTTCCGCC GAGTCCCTGG GCTCCGAGGC CAGCGCCACG 
TCGGGCTCCT CAGCCCCAGG GGAAAGCCGA AGCCGGCTCC GCTGGGGCTT CTCTCGGCCG 
CGGAAGGACA AGGGGTTATC GCCACCAAAC CTCTCTGCCA GCGTCCAGGA GGAGTTGGGT 
CACCAGTACG TGCGCAGTGA GTCAGTACCC TCACTTGGCC TGGAGGCAGC CATTGAGAAA 
CTGTGTTCAC ATTGCCTTGT TGGAACCTCA GTGTGGGACC CCTCCTTGGG GAGCAGTGGG 
GTACAGCGGG AAGGGGGCAC ACTGCCATCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_017003159.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858648.1 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X8, mRNA.

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CloneID	OHu88920
Clone ID Related Accession (Same CDS sequence)	XM_017003159.1 , XM_017003159.2
Accession Version	XM_017003159.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017003159.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCG CCACCAACGA GCTGGGCCAG GCCACCTGTG CCGCCTCACT GACCGTGAGA 
CCCGGTGGGT CTACATCCCC TTTCAGCAGC CCCATCACCT CCGACGAGGA ATACCTGAGC 
CCCCCAGAGG AGTTCCCAGA GCCTGGGGAG ACCTGGCCGC GAACCCCCAC CATGAAGCCC 
AGTCCCAGCC AGAACCGCCG TTCTTCTGAC ACTGGCTCCA AGGCACCCCC CACCTTCAAG 
GTCTCACTTA TGGACCAGTC AGTAAGAGAA GGCCAAGATG TCATCATGAG CATCCGCGTG 
CAGGGGGAGC CCAAGCCTGT GGTCTCCTGG CTGAGAAACC GCCAGCCCGT GCGCCCAGAC 
CAGCGGCGCT TTGCGGAGGA GGCTGAGGGT GGGCTGTGCC GGCTGCGGAT CCTGGCTGCA 
GAGCGTGGCG ATGCTGGTTT CTACACTTGC AAAGCGGTCA ATGAGTATGG TGCTCGGCAG 
TGCGAGGCCC GCTTGGAGGT CCGAGCACAC CCTGAAAGCC GGTCCCTGGC CGTGCTGGCC 
CCCCTGCAGG ACGTGGACGT GGGGGCCGGG GAGATGGCGC TGTTTGAGTG CCTGGTGGCG 
GGGCCCACTG ACGTGGAGGT GGATTGGCTG TGCCGTGGCC GCCTGCTGCA GCCTGCACTG 
CTCAAATGCA AGATGCATTT CGATGGCCGC AAATGCAAGC TGCTACTTAC ATCTGTACAT 
GAGGACGACA GTGGCGTCTA CACCTGCAAG CTCAGCACGG CCAAAGATGA GCTGACCTGC 
AGTGCCCGGC TGACCGTGCG GCCCTCGTTG GCACCCCTGT TCACACGGCT GCTGGAAGAT 
GTGGAGGTGT TGGAGGGCCG AGCTGCCCGT TTCGACTGCA AGATCAGTGG CACCCCGCCC 
CCTGTTGTTA CCTGGACTCA TTTTGGCTGC CCCATGGAGG AGAGTGAGAA CTTGCGGCTG 
CGGCAGGACG GGGGTCTGCA CTCACTGCAC ATTGCCCATG TGGGCAGCGA GGACGAGGGG 
CTCTATGCGG TCAGTGCTGT TAACACCCAT GGCCAGGCCC ACTGCTCAGC CCAGCTGTAT 
GTAGAAGAGC CCCGGACAGC CGCCTCAGGC CCCAGCTCGA AGCTGGAGAA GATGCCATCC 
ATTCCCGAGG AGCCAGAGCA GGGTGAGCTG GAGCGGCTGT CCATTCCTGA CTTCCTGCGG 
CCACTGCAGG ACCTGGAGGT GGGACTGGCC AAGGAGGCCA TGCTAGAGTG CCAGGTGACC 
GGCCTGCCCT ACCCCACCAT CAGCTGGTTC CACAATGGCC ACCGCATCCA GAGCAGCGAC 
GACCGGCGCA TGACACAGTA CAGGGATGTC CATCGCTTGG TGTTCCCTGC CGTGGGGCCT 
CAGCACGCCG GTGTCTACAA GAGCGTCATT GCCAACAAGC TGGGCAAAGC TGCCTGCTAT 
GCCCACCTGT ATGTCACAGA TGTGGTCCCA GGCCCTCCAG ATGGCGCCCC GCAGGTGGTG 
GCTGTGACGG GGAGGATGGT CACACTCACA TGGAACCCCC CCAGGAGTCT GGACATGGCC 
ATCGACCCGG ACTCCCTGAC GTACACAGTG CAGCACCAGG TGCTGGGCTC GGACCAGTGG 
ACGGCACTGG TCACAGGCCT GCGGGAGCCA GGGTGGGCAG CCACAGGGCT GCGTAAGGGG 
GTCCAGCACA TCTTCCGGGT CCTCAGCACC ACTGTCAAGA GCAGCAGCAA GCCCTCACCC 
CCTTCTGAGC CTGTGCAGCT GCTGGAGCAC GGCCCAACCC TGGAGGAGGC CCCTGCCATG 
CTGGACAAAC CAGACATCGT GTATGTGGTG GAGGGACAGC CTGCCAGCGT CACCGTCACA 
TTCAACCATG TGGAGGCCCA GGTCGTCTGG AGGAGCTGCC GAGGGGCCCT CCTAGAGGCA 
CGGGCCGGTG TGTACGAGCT GAGCCAGCCA GATGATGACC AGTACTGTCT TCGGATCTGC 
CGGGTGAGCC GCCGGGACAT GGGGGCCCTC ACCTGCACCG CCCGAAACCG TCACGGCACA 
CAGACCTGCT CGGTCACATT GGAGCTGGCA GAGGCCCCTC GGTTTGAGTC CATCATGGAG 
GACGTGGAGG TGGGGGCTGG GGAAACTGCT CGCTTTGCGG TGGTGGTCGA GGGAAAACCA 
CTGCCGGACA TCATGTGGTA CAAGGACGAG GTGCTGCTGA CCGAGAGCAG CCATGTGAGC 
TTCGTGTACG AGGAGAATGA GTGCTCCCTG GTGGTGCTCA GCACGGGGGC CCAGGATGGA 
GGCGTCTACA CCTGCACCGC CCAGAACCTG GCGGGTGAGG TCTCCTGCAA AGCAGAGTTG 
GCTGTGCATT CAGCTCAGAC AGCTATGGAG GTCGAGGGGG TCGGGGAGGA TGAGGACCAT 
CGAGGAAGGA GACTCAGCGA CTTTTATGAC ATCCACCAGG AGATCGGCAG GGGTGCTTTC 
TCCTACTTGC GGCGCATAGT GGAGCGTAGC TCCGGCCTGG AGTTTGCGGC CAAGTTCATC 
CCCAGCCAGG CCAAGCCAAA GGCATCAGCG CGTCGGGAGG CCCGGCTGCT GGCCAGGCTC 
CAGCACGACT GTGTCCTCTA CTTCCATGAG GCCTTCGAGA GGCGCCGGGG ACTGGTCATT 
GTCACCGAGC TCTGCACAGA GGAGCTGCTG GAGCGAATCG CCAGGAAACC CACCGTGTGT 
GAGTCTGAGA TCCGGGCCTA TATGCGGCAG GTGCTAGAGG GAATACACTA CCTGCACCAG 
AGCCACGTGC TGCACCTCGA TGTCAAGCCT GAGAACCTGC TGGTGTGGGA TGGTGCTGCG 
GGCGAGCAGC AGGTGCGGAT CTGTGACTTT GGGAATGCCC AGGAGCTGAC TCCAGGAGAG 
CCCCAGTACT GCCAGTATGG CACACCTGAG TTTGTAGCAC CCGAGATTGT CAATCAGAGC 
CCCGTGTCTG GAGTCACTGA CATCTGGCCT GTGGGTGTTG TTGCCTTCCT CTGTCTGACA 
GGAATCTCCC CGTTTGTTGG GGAAAATGAC CGGACAACAT TGATGAACAT CCGAAACTAC 
AACGTGGCCT TCGAGGAGAC CACATTCCTG AGCCTGAGCA GGGAGGCCCG GGGCTTCCTC 
ATCAAAGTGT TGGTGCAGGA CCGGCTGAGA CCTACCGCAG AAGAGACCCT AGAACATCCT 
TGGTTCAAAA CTCAGGCAAA GGGCGCAGAG GTGAGCACGG ATCACCTGAA GCTATTCCTC 
TCCCGGCGGA GGTGGCAGCG CTCCCAGATC AGCTACAAAT GCCACCTGGT GCTGCGCCCC 
ATCCCCGAGC TGCTGCGGGC CCCCCCAGAG CGGGTGTGGG TGACCATGCC CAGAAGGCCA 
CCCCCCAGTG GGGGGCTCTC ATCCTCCTCG GATTCTGAAG AGGAAGAGCT GGAAGAGCTG 
CCCTCAGTGC CCCGCCCACT GCAGCCCGAG TTCTCTGGCT CCCGGGTGTC CCTCACAGAC 
ATTCCCACTG AGGATGAGGC CCTGGGGACC CCAGAGACTG GGGCTGCCAC CCCCATGGAC 
TGGCAGGAGC AGGGAAGGGC TCCCTCTCAG GACCAGGAGG CTCCCAGCCC AGAGGCCCTC 
CCCTCCCCAG GCCAGGAGCC CGCAGCTGGG GCTAGCCCCA GGCGGGGAGA GCTCCGCAGG 
GGCAGCTCGG CTGAGAGCGC CCTGCCCCGG GCCGGGCCGC GGGAGCTGGG CCGGGGCCTG 
CACAAGGCGG CGTCTGTGGA GCTGCCGCAG CGCCGGAGCC CCAGCCCGGG AGCCACCCGC 
CTGGCCCGGG GAGGCCTGGG TGAGGGCGAG TATGCCCAGA GGCTGCAGGC CCTGCGCCAG 
CGGCTGCTGC GGGGAGGCCC CGAGGATGGC AAGGTCAGCG GCCTCAGGGG TCCCCTGCTG 
GAGAGCCTGG GGGGCCGTGC TCGGGACCCC CGGATGGCAC GAGCTGCCTC CAGCGAGGCA 
GCGCCCCACC ACCAGCCCCC ACTCGAGAAC CGGGGCCTGC AAAAGAGCAG CAGCTTCTCC 
CAGGGTGAGG CGGAGCCCCG GGGCCGGCAC CGCCGAGCGG GGGCGCCCCT CGAGATCCCC 
GTGGCCAGGC TTGGGGCCCG TAGGCTACAG GAGTCTCCTT CCCTGTCTGC CCTCAGCGAG 
GCCCAGCCAT CCAGCCCTGC ACGGCCCAGC GCCCCCAAAC CCAGTACCCC TAAGTCTGCA 
GAACCTTCTG CCACCACACC TAGTGATGCT CCGCAGCCCC CCGCACCCCA GCCTGCCCAA 
GACAAGGCTC CAGAGCCCAG GCCAGAACCA GTCCGAGCCT CCAAGCCTGC ACCACCCCCC 
CAGGCCCTGC AAACCCTAGC GCTGCCCCTC ACACCCTATG CTCAGATCAT TCAGTCCCTC 
CAGCTGTCAG GCCACGCCCA GGGCCCCTCG CAGGGCCCTG CCGCGCCGCC TTCAGAGCCC 
AAGCCCCACG CTGCTGTCTT TGCCAGGGTG GCCTCCCCAC CTCCGGGAGC CCCCGAGAAG 
CGCGTGCCCT CAGCCGGGGG TCCCCCGGTG CTAGCCGAGA AAGCCCGAGT TCCCACGGTG 
CCCCCCAGGC CAGGCAGCAG TCTCAGTAGC AGCATCGAAA ACTTGGAGTC GGAGGCCGTG 
TTCGAGGCCA AGTTCAAGCG CAGCCGCGAG TCGCCCCTGT CGCTGGGGCT GCGGCTGCTG 
AGCCGTTCGC GCTCGGAGGA GCGCGGCCCC TTCCGTGGGG CCGAGGAGGA GGATGGCATA 
TACCGGCCCA GCCCGGCGGG GACCCCGCTG GAGCTGGTGC GACGGCCTGA GCGCTCACGC 
TCGGTGCAGG ACCTCAGGGC TGTCGGAGAG CCTGGCCTCG TCCGCCGCCT CTCGCTGTCA 
CTGTCCCAGC GGCTGCGGCG GACCCCTCCC GCGCAGCGCC ACCCGGCCTG GGAGGCCCGC 
GGCGGGGACG GAGAGAGCTC GGAGGGCGGG AGCTCGGCGC GGGGCTCCCC GGTGCTGGCG 
ATGCGCAGGC GGCTGAGCTT CACCCTGGAG CGGCTGTCCA GCCGATTGCA GCGCAGTGGC 
AGCAGCGAGG ACTCGGGGGG CGCGTCGGGC CGCAGCACGC CGCTGTTCGG ACGGCTTCGC 
AGGGCCACGT CCGAGGGCGA GAGTCTGCGG CGCCTTGGCC TTCCGCACAA CCAGTTGGCC 
GCCCAGGCCG GCGCCACCAC GCCTTCCGCC GAGTCCCTGG GCTCCGAGGC CAGCGCCACG 
TCGGGCTCCT CAGCCCCAGG GGAAAGCCGA AGCCGGCTCC GCTGGGGCTT CTCTCGGCCG 
CGGAAGGACA AGGGGTTATC GCCACCAAAC CTCTCTGCCA GCGTCCAGGA GGAGTTGGGT 
CACCAGTACG TGCGCAGTGA GTCAGTACCC TCACTTGGCC TGGAGGCAGC CATTGAGAAA 
CTGTGTTCAC ATTGCCTTGT TGGAACCTCA GTGTGGGACC CCTCCTTGGG GAGCAGTGGG 
GTACAGCGGG AAGGGGGCAC ACTGCCATCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858648.1
CDS	1669..7161
Translation	MATATNELGQATCAASLTVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKPSPSQNRRSSDTGSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPTDVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARFDCKISGTPPPVVTWTHFGCPMEESENLRLRQDGGLHSLHIAHVGSEDEGLYAVSAVNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEQGELERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDVHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPQVVAVTGRMVTLTWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPGWAATGLRKGVQHIFRVLSTTVKSSSKPSPPSEPVQLLEHGPTLEEAPAMLDKPDIVYVVEGQPASVTVTFNHVEAQVVWRSCRGALLEARAGVYELSQPDDDQYCLRICRVSRRDMGALTCTARNRHGTQTCSVTLELAEAPRFESIMEDVEVGAGETARFAVVVEGKPLPDIMWYKDEVLLTESSHVSFVYEENECSLVVLSTGAQDGGVYTCTAQNLAGEVSCKAELAVHSAQTAMEVEGVGEDEDHRGRRLSDFYDIHQEIGRGAFSYLRRIVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHDCVLYFHEAFERRRGLVIVTELCTEELLERIARKPTVCESEIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTQAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGELRRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQPPAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPSEPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEAKFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAVGEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAESLGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESVPSLGLEAAIEKLCSHCLVGTSVWDPSLGSSGVQREGGTLPS

Target ORF information:

RefSeq Version	XM_017003159.2
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003159.2

ORF Insert Sequence:

ATGGCCACCG CCACCAACGA GCTGGGCCAG GCCACCTGTG CCGCCTCACT GACCGTGAGA 
CCCGGTGGGT CTACATCCCC TTTCAGCAGC CCCATCACCT CCGACGAGGA ATACCTGAGC 
CCCCCAGAGG AGTTCCCAGA GCCTGGGGAG ACCTGGCCGC GAACCCCCAC CATGAAGCCC 
AGTCCCAGCC AGAACCGCCG TTCTTCTGAC ACTGGCTCCA AGGCACCCCC CACCTTCAAG 
GTCTCACTTA TGGACCAGTC AGTAAGAGAA GGCCAAGATG TCATCATGAG CATCCGCGTG 
CAGGGGGAGC CCAAGCCTGT GGTCTCCTGG CTGAGAAACC GCCAGCCCGT GCGCCCAGAC 
CAGCGGCGCT TTGCGGAGGA GGCTGAGGGT GGGCTGTGCC GGCTGCGGAT CCTGGCTGCA 
GAGCGTGGCG ATGCTGGTTT CTACACTTGC AAAGCGGTCA ATGAGTATGG TGCTCGGCAG 
TGCGAGGCCC GCTTGGAGGT CCGAGCACAC CCTGAAAGCC GGTCCCTGGC CGTGCTGGCC 
CCCCTGCAGG ACGTGGACGT GGGGGCCGGG GAGATGGCGC TGTTTGAGTG CCTGGTGGCG 
GGGCCCACTG ACGTGGAGGT GGATTGGCTG TGCCGTGGCC GCCTGCTGCA GCCTGCACTG 
CTCAAATGCA AGATGCATTT CGATGGCCGC AAATGCAAGC TGCTACTTAC ATCTGTACAT 
GAGGACGACA GTGGCGTCTA CACCTGCAAG CTCAGCACGG CCAAAGATGA GCTGACCTGC 
AGTGCCCGGC TGACCGTGCG GCCCTCGTTG GCACCCCTGT TCACACGGCT GCTGGAAGAT 
GTGGAGGTGT TGGAGGGCCG AGCTGCCCGT TTCGACTGCA AGATCAGTGG CACCCCGCCC 
CCTGTTGTTA CCTGGACTCA TTTTGGCTGC CCCATGGAGG AGAGTGAGAA CTTGCGGCTG 
CGGCAGGACG GGGGTCTGCA CTCACTGCAC ATTGCCCATG TGGGCAGCGA GGACGAGGGG 
CTCTATGCGG TCAGTGCTGT TAACACCCAT GGCCAGGCCC ACTGCTCAGC CCAGCTGTAT 
GTAGAAGAGC CCCGGACAGC CGCCTCAGGC CCCAGCTCGA AGCTGGAGAA GATGCCATCC 
ATTCCCGAGG AGCCAGAGCA GGGTGAGCTG GAGCGGCTGT CCATTCCTGA CTTCCTGCGG 
CCACTGCAGG ACCTGGAGGT GGGACTGGCC AAGGAGGCCA TGCTAGAGTG CCAGGTGACC 
GGCCTGCCCT ACCCCACCAT CAGCTGGTTC CACAATGGCC ACCGCATCCA GAGCAGCGAC 
GACCGGCGCA TGACACAGTA CAGGGATGTC CATCGCTTGG TGTTCCCTGC CGTGGGGCCT 
CAGCACGCCG GTGTCTACAA GAGCGTCATT GCCAACAAGC TGGGCAAAGC TGCCTGCTAT 
GCCCACCTGT ATGTCACAGA TGTGGTCCCA GGCCCTCCAG ATGGCGCCCC GCAGGTGGTG 
GCTGTGACGG GGAGGATGGT CACACTCACA TGGAACCCCC CCAGGAGTCT GGACATGGCC 
ATCGACCCGG ACTCCCTGAC GTACACAGTG CAGCACCAGG TGCTGGGCTC GGACCAGTGG 
ACGGCACTGG TCACAGGCCT GCGGGAGCCA GGGTGGGCAG CCACAGGGCT GCGTAAGGGG 
GTCCAGCACA TCTTCCGGGT CCTCAGCACC ACTGTCAAGA GCAGCAGCAA GCCCTCACCC 
CCTTCTGAGC CTGTGCAGCT GCTGGAGCAC GGCCCAACCC TGGAGGAGGC CCCTGCCATG 
CTGGACAAAC CAGACATCGT GTATGTGGTG GAGGGACAGC CTGCCAGCGT CACCGTCACA 
TTCAACCATG TGGAGGCCCA GGTCGTCTGG AGGAGCTGCC GAGGGGCCCT CCTAGAGGCA 
CGGGCCGGTG TGTACGAGCT GAGCCAGCCA GATGATGACC AGTACTGTCT TCGGATCTGC 
CGGGTGAGCC GCCGGGACAT GGGGGCCCTC ACCTGCACCG CCCGAAACCG TCACGGCACA 
CAGACCTGCT CGGTCACATT GGAGCTGGCA GAGGCCCCTC GGTTTGAGTC CATCATGGAG 
GACGTGGAGG TGGGGGCTGG GGAAACTGCT CGCTTTGCGG TGGTGGTCGA GGGAAAACCA 
CTGCCGGACA TCATGTGGTA CAAGGACGAG GTGCTGCTGA CCGAGAGCAG CCATGTGAGC 
TTCGTGTACG AGGAGAATGA GTGCTCCCTG GTGGTGCTCA GCACGGGGGC CCAGGATGGA 
GGCGTCTACA CCTGCACCGC CCAGAACCTG GCGGGTGAGG TCTCCTGCAA AGCAGAGTTG 
GCTGTGCATT CAGCTCAGAC AGCTATGGAG GTCGAGGGGG TCGGGGAGGA TGAGGACCAT 
CGAGGAAGGA GACTCAGCGA CTTTTATGAC ATCCACCAGG AGATCGGCAG GGGTGCTTTC 
TCCTACTTGC GGCGCATAGT GGAGCGTAGC TCCGGCCTGG AGTTTGCGGC CAAGTTCATC 
CCCAGCCAGG CCAAGCCAAA GGCATCAGCG CGTCGGGAGG CCCGGCTGCT GGCCAGGCTC 
CAGCACGACT GTGTCCTCTA CTTCCATGAG GCCTTCGAGA GGCGCCGGGG ACTGGTCATT 
GTCACCGAGC TCTGCACAGA GGAGCTGCTG GAGCGAATCG CCAGGAAACC CACCGTGTGT 
GAGTCTGAGA TCCGGGCCTA TATGCGGCAG GTGCTAGAGG GAATACACTA CCTGCACCAG 
AGCCACGTGC TGCACCTCGA TGTCAAGCCT GAGAACCTGC TGGTGTGGGA TGGTGCTGCG 
GGCGAGCAGC AGGTGCGGAT CTGTGACTTT GGGAATGCCC AGGAGCTGAC TCCAGGAGAG 
CCCCAGTACT GCCAGTATGG CACACCTGAG TTTGTAGCAC CCGAGATTGT CAATCAGAGC 
CCCGTGTCTG GAGTCACTGA CATCTGGCCT GTGGGTGTTG TTGCCTTCCT CTGTCTGACA 
GGAATCTCCC CGTTTGTTGG GGAAAATGAC CGGACAACAT TGATGAACAT CCGAAACTAC 
AACGTGGCCT TCGAGGAGAC CACATTCCTG AGCCTGAGCA GGGAGGCCCG GGGCTTCCTC 
ATCAAAGTGT TGGTGCAGGA CCGGCTGAGA CCTACCGCAG AAGAGACCCT AGAACATCCT 
TGGTTCAAAA CTCAGGCAAA GGGCGCAGAG GTGAGCACGG ATCACCTGAA GCTATTCCTC 
TCCCGGCGGA GGTGGCAGCG CTCCCAGATC AGCTACAAAT GCCACCTGGT GCTGCGCCCC 
ATCCCCGAGC TGCTGCGGGC CCCCCCAGAG CGGGTGTGGG TGACCATGCC CAGAAGGCCA 
CCCCCCAGTG GGGGGCTCTC ATCCTCCTCG GATTCTGAAG AGGAAGAGCT GGAAGAGCTG 
CCCTCAGTGC CCCGCCCACT GCAGCCCGAG TTCTCTGGCT CCCGGGTGTC CCTCACAGAC 
ATTCCCACTG AGGATGAGGC CCTGGGGACC CCAGAGACTG GGGCTGCCAC CCCCATGGAC 
TGGCAGGAGC AGGGAAGGGC TCCCTCTCAG GACCAGGAGG CTCCCAGCCC AGAGGCCCTC 
CCCTCCCCAG GCCAGGAGCC CGCAGCTGGG GCTAGCCCCA GGCGGGGAGA GCTCCGCAGG 
GGCAGCTCGG CTGAGAGCGC CCTGCCCCGG GCCGGGCCGC GGGAGCTGGG CCGGGGCCTG 
CACAAGGCGG CGTCTGTGGA GCTGCCGCAG CGCCGGAGCC CCAGCCCGGG AGCCACCCGC 
CTGGCCCGGG GAGGCCTGGG TGAGGGCGAG TATGCCCAGA GGCTGCAGGC CCTGCGCCAG 
CGGCTGCTGC GGGGAGGCCC CGAGGATGGC AAGGTCAGCG GCCTCAGGGG TCCCCTGCTG 
GAGAGCCTGG GGGGCCGTGC TCGGGACCCC CGGATGGCAC GAGCTGCCTC CAGCGAGGCA 
GCGCCCCACC ACCAGCCCCC ACTCGAGAAC CGGGGCCTGC AAAAGAGCAG CAGCTTCTCC 
CAGGGTGAGG CGGAGCCCCG GGGCCGGCAC CGCCGAGCGG GGGCGCCCCT CGAGATCCCC 
GTGGCCAGGC TTGGGGCCCG TAGGCTACAG GAGTCTCCTT CCCTGTCTGC CCTCAGCGAG 
GCCCAGCCAT CCAGCCCTGC ACGGCCCAGC GCCCCCAAAC CCAGTACCCC TAAGTCTGCA 
GAACCTTCTG CCACCACACC TAGTGATGCT CCGCAGCCCC CCGCACCCCA GCCTGCCCAA 
GACAAGGCTC CAGAGCCCAG GCCAGAACCA GTCCGAGCCT CCAAGCCTGC ACCACCCCCC 
CAGGCCCTGC AAACCCTAGC GCTGCCCCTC ACACCCTATG CTCAGATCAT TCAGTCCCTC 
CAGCTGTCAG GCCACGCCCA GGGCCCCTCG CAGGGCCCTG CCGCGCCGCC TTCAGAGCCC 
AAGCCCCACG CTGCTGTCTT TGCCAGGGTG GCCTCCCCAC CTCCGGGAGC CCCCGAGAAG 
CGCGTGCCCT CAGCCGGGGG TCCCCCGGTG CTAGCCGAGA AAGCCCGAGT TCCCACGGTG 
CCCCCCAGGC CAGGCAGCAG TCTCAGTAGC AGCATCGAAA ACTTGGAGTC GGAGGCCGTG 
TTCGAGGCCA AGTTCAAGCG CAGCCGCGAG TCGCCCCTGT CGCTGGGGCT GCGGCTGCTG 
AGCCGTTCGC GCTCGGAGGA GCGCGGCCCC TTCCGTGGGG CCGAGGAGGA GGATGGCATA 
TACCGGCCCA GCCCGGCGGG GACCCCGCTG GAGCTGGTGC GACGGCCTGA GCGCTCACGC 
TCGGTGCAGG ACCTCAGGGC TGTCGGAGAG CCTGGCCTCG TCCGCCGCCT CTCGCTGTCA 
CTGTCCCAGC GGCTGCGGCG GACCCCTCCC GCGCAGCGCC ACCCGGCCTG GGAGGCCCGC 
GGCGGGGACG GAGAGAGCTC GGAGGGCGGG AGCTCGGCGC GGGGCTCCCC GGTGCTGGCG 
ATGCGCAGGC GGCTGAGCTT CACCCTGGAG CGGCTGTCCA GCCGATTGCA GCGCAGTGGC 
AGCAGCGAGG ACTCGGGGGG CGCGTCGGGC CGCAGCACGC CGCTGTTCGG ACGGCTTCGC 
AGGGCCACGT CCGAGGGCGA GAGTCTGCGG CGCCTTGGCC TTCCGCACAA CCAGTTGGCC 
GCCCAGGCCG GCGCCACCAC GCCTTCCGCC GAGTCCCTGG GCTCCGAGGC CAGCGCCACG 
TCGGGCTCCT CAGCCCCAGG GGAAAGCCGA AGCCGGCTCC GCTGGGGCTT CTCTCGGCCG 
CGGAAGGACA AGGGGTTATC GCCACCAAAC CTCTCTGCCA GCGTCCAGGA GGAGTTGGGT 
CACCAGTACG TGCGCAGTGA GTCAGTACCC TCACTTGGCC TGGAGGCAGC CATTGAGAAA 
CTGTGTTCAC ATTGCCTTGT TGGAACCTCA GTGTGGGACC CCTCCTTGGG GAGCAGTGGG 
GTACAGCGGG AAGGGGGCAC ACTGCCATCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEG ( XM_017003160.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858649.1 Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X14, mRNA.

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CloneID	OHu88921
Clone ID Related Accession (Same CDS sequence)	XM_017003160.1
Accession Version	XM_017003160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	striated muscle preferentially expressed protein kinase isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCTT CGAGAGGCGC CGGGGACTGG TCATTGTCAC CGAGCTCTGC ACAGAGGAGC 
TGCTGGAGCG AATCGCCAGG AAACCCACCG TGTATCCGGG CCTATATGCG GCAGGTGCTA 
GAGGGAATAC ACTACCTGCA CCAGAGCCAC GTGCTGCACC TCGATGTCAA GCCTGAGAAC 
CTGCTGGTGT GGGATGGTGC TGCGGGCGAG CAGCAGGTGC GGATCTGTGA CTTTGGGAAT 
GCCCAGGAGC TGACTCCAGG AGAGCCCCAG TACTGCCAGT ATGGCACACC TGAGTTTGTA 
GCACCCGAGA TTGTCAATCA GAGCCCCGTG TCTGGAGTCA CTGACATCTG GCCTGTGGGT 
GTTGTTGCCT TCCTCTGTCT GACAGGAATC TCCCCGTTTG TTGGGGAAAA TGACCGGACA 
ACATTGATGA ACATCCGAAA CTACAACGTG GCCTTCGAGG AGACCACATT CCTGAGCCTG 
AGCAGGGAGG CCCGGGGCTT CCTCATCAAA GTGTTGGTGC AGGACCGGCT GAGACCTACC 
GCAGAAGAGA CCCTAGAACA TCCTTGGTTC AAAACTCAGG CAAAGGGCGC AGAGGTGAGC 
ACGGATCACC TGAAGCTATT CCTCTCCCGG CGGAGGTGGC AGCGCTCCCA GATCAGCTAC 
AAATGCCACC TGGTGCTGCG CCCCATCCCC GAGCTGCTGC GGGCCCCCCC AGAGCGGGTG 
TGGGTGACCA TGCCCAGAAG GCCACCCCCC AGTGGGGGGC TCTCATCCTC CTCGGATTCT 
GAAGAGGAAG AGCTGGAAGA GCTGCCCTCA GTGCCCCGCC CACTGCAGCC CGAGTTCTCT 
GGCTCCCGGG TGTCCCTCAC AGACATTCCC ACTGAGGATG AGGCCCTGGG GACCCCAGAG 
ACTGGGGCTG CCACCCCCAT GGACTGGCAG GAGCAGGGAA GGGCTCCCTC TCAGGACCAG 
GAGGCTCCCA GCCCAGAGGC CCTCCCCTCC CCAGGCCAGG AGCCCGCAGC TGGGGCTAGC 
CCCAGGCGGG GAGAGCTCCG CAGGGGCAGC TCGGCTGAGA GCGCCCTGCC CCGGGCCGGG 
CCGCGGGAGC TGGGCCGGGG CCTGCACAAG GCGGCGTCTG TGGAGCTGCC GCAGCGCCGG 
AGCCCCAGCC CGGGAGCCAC CCGCCTGGCC CGGGGAGGCC TGGGTGAGGG CGAGTATGCC 
CAGAGGCTGC AGGCCCTGCG CCAGCGGCTG CTGCGGGGAG GCCCCGAGGA TGGCAAGGTC 
AGCGGCCTCA GGGGTCCCCT GCTGGAGAGC CTGGGGGGCC GTGCTCGGGA CCCCCGGATG 
GCACGAGCTG CCTCCAGCGA GGCAGCGCCC CACCACCAGC CCCCACTCGA GAACCGGGGC 
CTGCAAAAGA GCAGCAGCTT CTCCCAGGGT GAGGCGGAGC CCCGGGGCCG GCACCGCCGA 
GCGGGGGCGC CCCTCGAGAT CCCCGTGGCC AGGCTTGGGG CCCGTAGGCT ACAGGAGTCT 
CCTTCCCTGT CTGCCCTCAG CGAGGCCCAG CCATCCAGCC CTGCACGGCC CAGCGCCCCC 
AAACCCAGTA CCCCTAAGTC TGCAGAACCT TCTGCCACCA CACCTAGTGA TGCTCCGCAG 
CCCCCCGCAC CCCAGCCTGC CCAAGACAAG GCTCCAGAGC CCAGGCCAGA ACCAGTCCGA 
GCCTCCAAGC CTGCACCACC CCCCCAGGCC CTGCAAACCC TAGCGCTGCC CCTCACACCC 
TATGCTCAGA TCATTCAGTC CCTCCAGCTG TCAGGCCACG CCCAGGGCCC CTCGCAGGGC 
CCTGCCGCGC CGCCTTCAGA GCCCAAGCCC CACGCTGCTG TCTTTGCCAG GGTGGCCTCC 
CCACCTCCGG GAGCCCCCGA GAAGCGCGTG CCCTCAGCCG GGGGTCCCCC GGTGCTAGCC 
GAGAAAGCCC GAGTTCCCAC GGTGCCCCCC AGGCCAGGCA GCAGTCTCAG TAGCAGCATC 
GAAAACTTGG AGTCGGAGGC CGTGTTCGAG GCCAAGTTCA AGCGCAGCCG CGAGTCGCCC 
CTGTCGCTGG GGCTGCGGCT GCTGAGCCGT TCGCGCTCGG AGGAGCGCGG CCCCTTCCGT 
GGGGCCGAGG AGGAGGATGG CATATACCGG CCCAGCCCGG CGGGGACCCC GCTGGAGCTG 
GTGCGACGGC CTGAGCGCTC ACGCTCGGTG CAGGACCTCA GGGCTGTCGG AGAGCCTGGC 
CTCGTCCGCC GCCTCTCGCT GTCACTGTCC CAGCGGCTGC GGCGGACCCC TCCCGCGCAG 
CGCCACCCGG CCTGGGAGGC CCGCGGCGGG GACGGAGAGA GCTCGGAGGG CGGGAGCTCG 
GCGCGGGGCT CCCCGGTGCT GGCGATGCGC AGGCGGCTGA GCTTCACCCT GGAGCGGCTG 
TCCAGCCGAT TGCAGCGCAG TGGCAGCAGC GAGGACTCGG GGGGCGCGTC GGGCCGCAGC 
ACGCCGCTGT TCGGACGGCT TCGCAGGGCC ACGTCCGAGG GCGAGAGTCT GCGGCGCCTT 
GGCCTTCCGC ACAACCAGTT GGCCGCCCAG GCCGGCGCCA CCACGCCTTC CGCCGAGTCC 
CTGGGCTCCG AGGCCAGCGC CACGTCGGGC TCCTCAGCCC CAGGGGAAAG CCGAAGCCGG 
CTCCGCTGGG GCTTCTCTCG GCCGCGGAAG GACAAGGGGT TATCGCCACC AAACCTCTCT 
GCCAGCGTCC AGGAGGAGTT GGGTCACCAG TACGTGCGCA GTGAGTCAGA CTTCCCCCCA 
GTCTTCCACA TCAAACTCAA GGACCAGGTG CTGCTGGAGG GGGAGGCAGC CACCCTGCTC 
TGCCTGCCAG CGGCCTGCCC TGCACCGCAC ATCTCCTGGA TGAAAGACAA GAAGTCCTTG 
AGGTCAGAGC CCTCAGTGAT CATCGTGTCC TGCAAAGATG GGCGGCAGCT GCTCAGCATC 
CCCCGGGCGG GCAAGCGGCA CGCCGGTCTC TATGAGTGCT CGGCCACCAA CGTACTGGGC 
AGCATCACCA GCTCCTGTAC CGTGGCTGTG GCCCGAGTCC CAGGAAAGCT AGCTCCTCCA 
GAGGTACCCC AGACCTACCA GGACACGGCG CTGGTGCTGT GGAAGCCGGG AGACAGCCGG 
GCACCTTGCA CGTATACGCT GGAGCGGCGA GTGGATGGGG AGTCTGTGTG GCACCCTGTG 
AGCTCAGGCA TCCCCGACTG TTACTACAAC GTGACCCACC TGCCAGTTGG CGTGACTGTG 
AGGTTCCGTG TGGCCTGTGC CAACCGTGCT GGGCAGGGGC CCTTCAGCAA CTCTTCTGAG 
AAGGTCTTTG TCAGGGGTAC TCAAGATTCT TCAGCTGTGC CATCTGCTGC CCACCAAGAG 
GCCCCTGTCA CCTCAAGGCC AGCCAGGGCC CGGCCTCCTG ACTCTCCTAC CTCACTGGCC 
CCACCCCTAG CTCCTGCTGC CCCCACACCC CCGTCAGTCA CTGTCAGCCC CTCATCTCCC 
CCCACACCTC CTAGCCAGGC CTTGTCCTCG CTCAAGGCTG TGGGTCCACC ACCCCAAACC 
CCTCCACGAA GACACAGGGG CCTGCAGGCT GCCCGGCCAG CGGAGCCCAC CCTACCCAGT 
ACCCACGTCA CCCCAAGTGA GCCCAAGCCT TTCGTCCTTG ACACTGGGAC CCCGATCCCA 
GCCTCCACTC CTCAAGGGGT TAAACCAGTG TCTTCCTCTA CTCCTGTGTA TGTGGTGACT 
TCCTTTGTGT CTGCACCACC AGCCCCTGAG CCCCCAGCCC CTGAGCCCCC TCCTGAGCCT 
ACCAAGGTGA CTGTGCAGAG CCTCAGCCCG GCCAAGGAGG TGGTCAGCTC CCCTGGGAGC 
AGTCCCCGAA GCTCTCCCAG GCCTGAGGGT ACCACTCTTC GACAGGGTCC CCCTCAGAAA 
CCCTACACCT TCCTGGAGGA GAAAGCCAGG GGCCGCTTTG GTGTTGTGCG AGCGTGCCGG 
GAGAATGCCA CGGGGCGAAC GTTCGTGGCC AAGATCGTGC CCTATGCTGC CGAGGGCAAG 
CGGCGGGTCC TGCAGGAGTA CGAGGTGCTG CGGACCCTGC ACCACGAGCG GATCATGTCC 
CTGCACGAGG CCTACATCAC CCCTCGGTAC CTCGTGCTCA TTGCTGAGAG CTGTGGCAAC 
CGGGAACTCC TCTGTGGGCT CAGTGACAGG TTCCGGTATT CTGAGGATGA CGTGGCCACT 
TACATGGTGC AGCTGCTACA AGGCCTGGAC TACCTCCACG GCCACCACGT GCTCCACCTA 
GACATCAAGC CAGACAACCT GCTGCTGGCC CCTGACAATG CCCTCAAGAT TGTGGACTTT 
GGCAGTGCCC AGCCCTACAA CCCCCAGGCC CTTAGGCCCC TTGGCCACCG CACGGGCACG 
CTGGAGTTCA TGGCTCCGGA GATGGTGAAG GGAGAACCCA TCGGCTCTGC CACGGACATC 
TGGGGAGCGG GTGTGCTCAC TTACATTATG CTCAGTGGAC GCTCCCCGTT CTATGAGCCA 
GACCCCCAGG AAACGGAGGC TCGGATTGTG GGGGGCCGCT TTGATGCCTT CCAGCTGTAC 
CCCAATACAT CCCAGAGCGC CACCCTCTTC TTGCGAAAGG TTCTCTCTGT ACATCCCTGG 
AGCCGGCCCT CCCTGCAGGA CTGCCTGGCC CACCCATGGT TGCAGGACGC CTACCTGATG 
AAGCTGCGCC GCCAGACGCT CACCTTCACC ACCAACCGGC TCAAGGAGTT CCTGGGCGAG 
CAGCGGCGGC GCCGGGCTGA GGCTGCCACC CGCCACAAGG TGCTGCTGCG CTCCTACCCT 
GGCGGCCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858649.1
CDS	928..5739
Translation	MRPSRGAGDWSLSPSSAQRSCWSESPGNPPCIRAYMRQVLEGIHYLHQSHVLHLDVKPENLLVWDGAAGEQQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTQAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVTMPRRPPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPETGAATPMDWQEQGRAPSQDQEAPSPEALPSPGQEPAAGASPRRGELRRGSSAESALPRAGPRELGRGLHKAASVELPQRRSPSPGATRLARGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPLENRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSEAQPSSPARPSAPKPSTPKSAEPSATTPSDAPQPPAPQPAQDKAPEPRPEPVRASKPAPPPQALQTLALPLTPYAQIIQSLQLSGHAQGPSQGPAAPPSEPKPHAAVFARVASPPPGAPEKRVPSAGGPPVLAEKARVPTVPPRPGSSLSSSIENLESEAVFEAKFKRSRESPLSLGLRLLSRSRSEERGPFRGAEEEDGIYRPSPAGTPLELVRRPERSRSVQDLRAVGEPGLVRRLSLSLSQRLRRTPPAQRHPAWEARGGDGESSEGGSSARGSPVLAMRRRLSFTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGLPHNQLAAQAGATTPSAESLGSEASATSGSSAPGESRSRLRWGFSRPRKDKGLSPPNLSASVQEELGHQYVRSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPHISWMKDKKSLRSEPSVIIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARVPGKLAPPEVPQTYQDTALVLWKPGDSRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTHLPVGVTVRFRVACANRAGQGPFSNSSEKVFVRGTQDSSAVPSAAHQEAPVTSRPARARPPDSPTSLAPPLAPAAPTPPSVTVSPSSPPTPPSQALSSLKAVGPPPQTPPRRHRGLQAARPAEPTLPSTHVTPSEPKPFVLDTGTPIPASTPQGVKPVSSSTPVYVVTSFVSAPPAPEPPAPEPPPEPTKVTVQSLSPAKEVVSSPGSSPRSSPRPEGTTLRQGPPQKPYTFLEEKARGRFGVVRACRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERIMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYMVQLLQGLDYLHGHHVLHLDIKPDNLLLAPDNALKIVDFGSAQPYNPQALRPLGHRTGTLEFMAPEMVKGEPIGSATDIWGAGVLTYIMLSGRSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGGP

Target ORF information:

RefSeq Version	XM_017003160.1
Organism	Homo sapiens(human)
Definition	Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003160.1

ORF Insert Sequence:

ATGAGGCCTT CGAGAGGCGC CGGGGACTGG TCATTGTCAC CGAGCTCTGC ACAGAGGAGC 
TGCTGGAGCG AATCGCCAGG AAACCCACCG TGTATCCGGG CCTATATGCG GCAGGTGCTA 
GAGGGAATAC ACTACCTGCA CCAGAGCCAC GTGCTGCACC TCGATGTCAA GCCTGAGAAC 
CTGCTGGTGT GGGATGGTGC TGCGGGCGAG CAGCAGGTGC GGATCTGTGA CTTTGGGAAT 
GCCCAGGAGC TGACTCCAGG AGAGCCCCAG TACTGCCAGT ATGGCACACC TGAGTTTGTA 
GCACCCGAGA TTGTCAATCA GAGCCCCGTG TCTGGAGTCA CTGACATCTG GCCTGTGGGT 
GTTGTTGCCT TCCTCTGTCT GACAGGAATC TCCCCGTTTG TTGGGGAAAA TGACCGGACA 
ACATTGATGA ACATCCGAAA CTACAACGTG GCCTTCGAGG AGACCACATT CCTGAGCCTG 
AGCAGGGAGG CCCGGGGCTT CCTCATCAAA GTGTTGGTGC AGGACCGGCT GAGACCTACC 
GCAGAAGAGA CCCTAGAACA TCCTTGGTTC AAAACTCAGG CAAAGGGCGC AGAGGTGAGC 
ACGGATCACC TGAAGCTATT CCTCTCCCGG CGGAGGTGGC AGCGCTCCCA GATCAGCTAC 
AAATGCCACC TGGTGCTGCG CCCCATCCCC GAGCTGCTGC GGGCCCCCCC AGAGCGGGTG 
TGGGTGACCA TGCCCAGAAG GCCACCCCCC AGTGGGGGGC TCTCATCCTC CTCGGATTCT 
GAAGAGGAAG AGCTGGAAGA GCTGCCCTCA GTGCCCCGCC CACTGCAGCC CGAGTTCTCT 
GGCTCCCGGG TGTCCCTCAC AGACATTCCC ACTGAGGATG AGGCCCTGGG GACCCCAGAG 
ACTGGGGCTG CCACCCCCAT GGACTGGCAG GAGCAGGGAA GGGCTCCCTC TCAGGACCAG 
GAGGCTCCCA GCCCAGAGGC CCTCCCCTCC CCAGGCCAGG AGCCCGCAGC TGGGGCTAGC 
CCCAGGCGGG GAGAGCTCCG CAGGGGCAGC TCGGCTGAGA GCGCCCTGCC CCGGGCCGGG 
CCGCGGGAGC TGGGCCGGGG CCTGCACAAG GCGGCGTCTG TGGAGCTGCC GCAGCGCCGG 
AGCCCCAGCC CGGGAGCCAC CCGCCTGGCC CGGGGAGGCC TGGGTGAGGG CGAGTATGCC 
CAGAGGCTGC AGGCCCTGCG CCAGCGGCTG CTGCGGGGAG GCCCCGAGGA TGGCAAGGTC 
AGCGGCCTCA GGGGTCCCCT GCTGGAGAGC CTGGGGGGCC GTGCTCGGGA CCCCCGGATG 
GCACGAGCTG CCTCCAGCGA GGCAGCGCCC CACCACCAGC CCCCACTCGA GAACCGGGGC 
CTGCAAAAGA GCAGCAGCTT CTCCCAGGGT GAGGCGGAGC CCCGGGGCCG GCACCGCCGA 
GCGGGGGCGC CCCTCGAGAT CCCCGTGGCC AGGCTTGGGG CCCGTAGGCT ACAGGAGTCT 
CCTTCCCTGT CTGCCCTCAG CGAGGCCCAG CCATCCAGCC CTGCACGGCC CAGCGCCCCC 
AAACCCAGTA CCCCTAAGTC TGCAGAACCT TCTGCCACCA CACCTAGTGA TGCTCCGCAG 
CCCCCCGCAC CCCAGCCTGC CCAAGACAAG GCTCCAGAGC CCAGGCCAGA ACCAGTCCGA 
GCCTCCAAGC CTGCACCACC CCCCCAGGCC CTGCAAACCC TAGCGCTGCC CCTCACACCC 
TATGCTCAGA TCATTCAGTC CCTCCAGCTG TCAGGCCACG CCCAGGGCCC CTCGCAGGGC 
CCTGCCGCGC CGCCTTCAGA GCCCAAGCCC CACGCTGCTG TCTTTGCCAG GGTGGCCTCC 
CCACCTCCGG GAGCCCCCGA GAAGCGCGTG CCCTCAGCCG GGGGTCCCCC GGTGCTAGCC 
GAGAAAGCCC GAGTTCCCAC GGTGCCCCCC AGGCCAGGCA GCAGTCTCAG TAGCAGCATC 
GAAAACTTGG AGTCGGAGGC CGTGTTCGAG GCCAAGTTCA AGCGCAGCCG CGAGTCGCCC 
CTGTCGCTGG GGCTGCGGCT GCTGAGCCGT TCGCGCTCGG AGGAGCGCGG CCCCTTCCGT 
GGGGCCGAGG AGGAGGATGG CATATACCGG CCCAGCCCGG CGGGGACCCC GCTGGAGCTG 
GTGCGACGGC CTGAGCGCTC ACGCTCGGTG CAGGACCTCA GGGCTGTCGG AGAGCCTGGC 
CTCGTCCGCC GCCTCTCGCT GTCACTGTCC CAGCGGCTGC GGCGGACCCC TCCCGCGCAG 
CGCCACCCGG CCTGGGAGGC CCGCGGCGGG GACGGAGAGA GCTCGGAGGG CGGGAGCTCG 
GCGCGGGGCT CCCCGGTGCT GGCGATGCGC AGGCGGCTGA GCTTCACCCT GGAGCGGCTG 
TCCAGCCGAT TGCAGCGCAG TGGCAGCAGC GAGGACTCGG GGGGCGCGTC GGGCCGCAGC 
ACGCCGCTGT TCGGACGGCT TCGCAGGGCC ACGTCCGAGG GCGAGAGTCT GCGGCGCCTT 
GGCCTTCCGC ACAACCAGTT GGCCGCCCAG GCCGGCGCCA CCACGCCTTC CGCCGAGTCC 
CTGGGCTCCG AGGCCAGCGC CACGTCGGGC TCCTCAGCCC CAGGGGAAAG CCGAAGCCGG 
CTCCGCTGGG GCTTCTCTCG GCCGCGGAAG GACAAGGGGT TATCGCCACC AAACCTCTCT 
GCCAGCGTCC AGGAGGAGTT GGGTCACCAG TACGTGCGCA GTGAGTCAGA CTTCCCCCCA 
GTCTTCCACA TCAAACTCAA GGACCAGGTG CTGCTGGAGG GGGAGGCAGC CACCCTGCTC 
TGCCTGCCAG CGGCCTGCCC TGCACCGCAC ATCTCCTGGA TGAAAGACAA GAAGTCCTTG 
AGGTCAGAGC CCTCAGTGAT CATCGTGTCC TGCAAAGATG GGCGGCAGCT GCTCAGCATC 
CCCCGGGCGG GCAAGCGGCA CGCCGGTCTC TATGAGTGCT CGGCCACCAA CGTACTGGGC 
AGCATCACCA GCTCCTGTAC CGTGGCTGTG GCCCGAGTCC CAGGAAAGCT AGCTCCTCCA 
GAGGTACCCC AGACCTACCA GGACACGGCG CTGGTGCTGT GGAAGCCGGG AGACAGCCGG 
GCACCTTGCA CGTATACGCT GGAGCGGCGA GTGGATGGGG AGTCTGTGTG GCACCCTGTG 
AGCTCAGGCA TCCCCGACTG TTACTACAAC GTGACCCACC TGCCAGTTGG CGTGACTGTG 
AGGTTCCGTG TGGCCTGTGC CAACCGTGCT GGGCAGGGGC CCTTCAGCAA CTCTTCTGAG 
AAGGTCTTTG TCAGGGGTAC TCAAGATTCT TCAGCTGTGC CATCTGCTGC CCACCAAGAG 
GCCCCTGTCA CCTCAAGGCC AGCCAGGGCC CGGCCTCCTG ACTCTCCTAC CTCACTGGCC 
CCACCCCTAG CTCCTGCTGC CCCCACACCC CCGTCAGTCA CTGTCAGCCC CTCATCTCCC 
CCCACACCTC CTAGCCAGGC CTTGTCCTCG CTCAAGGCTG TGGGTCCACC ACCCCAAACC 
CCTCCACGAA GACACAGGGG CCTGCAGGCT GCCCGGCCAG CGGAGCCCAC CCTACCCAGT 
ACCCACGTCA CCCCAAGTGA GCCCAAGCCT TTCGTCCTTG ACACTGGGAC CCCGATCCCA 
GCCTCCACTC CTCAAGGGGT TAAACCAGTG TCTTCCTCTA CTCCTGTGTA TGTGGTGACT 
TCCTTTGTGT CTGCACCACC AGCCCCTGAG CCCCCAGCCC CTGAGCCCCC TCCTGAGCCT 
ACCAAGGTGA CTGTGCAGAG CCTCAGCCCG GCCAAGGAGG TGGTCAGCTC CCCTGGGAGC 
AGTCCCCGAA GCTCTCCCAG GCCTGAGGGT ACCACTCTTC GACAGGGTCC CCCTCAGAAA 
CCCTACACCT TCCTGGAGGA GAAAGCCAGG GGCCGCTTTG GTGTTGTGCG AGCGTGCCGG 
GAGAATGCCA CGGGGCGAAC GTTCGTGGCC AAGATCGTGC CCTATGCTGC CGAGGGCAAG 
CGGCGGGTCC TGCAGGAGTA CGAGGTGCTG CGGACCCTGC ACCACGAGCG GATCATGTCC 
CTGCACGAGG CCTACATCAC CCCTCGGTAC CTCGTGCTCA TTGCTGAGAG CTGTGGCAAC 
CGGGAACTCC TCTGTGGGCT CAGTGACAGG TTCCGGTATT CTGAGGATGA CGTGGCCACT 
TACATGGTGC AGCTGCTACA AGGCCTGGAC TACCTCCACG GCCACCACGT GCTCCACCTA 
GACATCAAGC CAGACAACCT GCTGCTGGCC CCTGACAATG CCCTCAAGAT TGTGGACTTT 
GGCAGTGCCC AGCCCTACAA CCCCCAGGCC CTTAGGCCCC TTGGCCACCG CACGGGCACG 
CTGGAGTTCA TGGCTCCGGA GATGGTGAAG GGAGAACCCA TCGGCTCTGC CACGGACATC 
TGGGGAGCGG GTGTGCTCAC TTACATTATG CTCAGTGGAC GCTCCCCGTT CTATGAGCCA 
GACCCCCAGG AAACGGAGGC TCGGATTGTG GGGGGCCGCT TTGATGCCTT CCAGCTGTAC 
CCCAATACAT CCCAGAGCGC CACCCTCTTC TTGCGAAAGG TTCTCTCTGT ACATCCCTGG 
AGCCGGCCCT CCCTGCAGGA CTGCCTGGCC CACCCATGGT TGCAGGACGC CTACCTGATG 
AAGCTGCGCC GCCAGACGCT CACCTTCACC ACCAACCGGC TCAAGGAGTT CCTGGGCGAG 
CAGCGGCGGC GCCGGGCTGA GGCTGCCACC CGCCACAAGG TGCTGCTGCG CTCCTACCCT 
GGCGGCCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SPEG (Striated Muscle Preferentially Expressed Protein Kinase) Is Essential for Cardiac Function by Regulating Junctional Membrane Complex Activity.
Circulation research120(1)110-119(2017 Jan)
Quick AP,Wang Q,Philippen LE,Barreto-Torres G,Chiang DY,Beavers D,Wang G,Khalid M,Reynolds JO,Campbell HM,Showell J,McCauley MD,Scholten A,Wehrens XH

SPEG interacts with myotubularin, and its deficiency causes centronuclear myopathy with dilated cardiomyopathy.
American journal of human genetics95(2)218-26(2014 Aug)
Agrawal PB,Pierson CR,Joshi M,Liu X,Ravenscroft G,Moghadaszadeh B,Talabere T,Viola M,Swanson LC,Halilo?lu G,Talim B,Yau KS,Allcock RJ,Laing NG,Perrella MA,Beggs AH

The human desmin locus: gene organization and LCR-mediated transcriptional control.
Genomics87(6)733-46(2006 Jun)
Tam JL,Triantaphyllopoulos K,Todd H,Raguz S,de Wit T,Morgan JE,Partridge TA,Makrinou E,Grosveld F,Antoniou M

X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1).
BMC structural biology521(2005 Dec)
Manjasetty BA,Niesen FH,Scheich C,Roske Y,Goetz F,Behlke J,Sievert V,Heinemann U,B?ssow K

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SPEG cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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