Speg cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Speg
Entrez Gene ID	11790
Full Name	SPEG complex locus
Synonyms	AW125581,Apeg1,BPEG,D1Bwg1450e,SPEGalpha,SPEGbeta,mKIAA1297
General protein information	Preferred Names SPEG complex locus Names striated muscle-specific serine/threonine-protein kinase APEG-1 aortic preferentially expressed gene 1 aortic preferentially expressed protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 C4\|1 38.88 cM
Summary	This gene encodes a protein with similarity to members of the myosin light chain kinase family. This protein family is required for myocyte cytoskeletal development. Studies have determined that a lack of this protein affected myocardial development. Multiple alternatively spliced transcript variants that encode different protein isoforms have been defined. [provided by RefSeq, Mar 2010].

mRNA and Protein(s)

mRNA	Protein	Name
XM_011238644.1	XP_011236946.1	striated muscle-specific serine/threonine-protein kinase isoform X2
XM_006496400.2	XP_006496463.1	striated muscle-specific serine/threonine-protein kinase isoform X8
XM_006496404.4	XP_006496467.1	striated muscle-specific serine/threonine-protein kinase isoform X11
XM_030243348.1	XP_030099208.1	striated muscle-specific serine/threonine-protein kinase isoform X12
XM_030243390.1	XP_030099250.1	striated muscle-specific serine/threonine-protein kinase isoform X15
XM_030243512.1	XP_030099372.1	striated muscle-specific serine/threonine-protein kinase isoform X19
NM_007463.4	NP_031489.4	striated muscle-specific serine/threonine-protein kinase isoform 1
XM_017322354.1	XP_017177843.1	striated muscle-specific serine/threonine-protein kinase isoform X3
XM_011238648.2	XP_011236950.1	striated muscle-specific serine/threonine-protein kinase isoform X8
XM_006496404.3	XP_006496467.1	striated muscle-specific serine/threonine-protein kinase isoform X10
XM_030243202.1	XP_030099062.1	striated muscle-specific serine/threonine-protein kinase isoform X2
XM_011238647.2	XP_011236949.1	striated muscle-specific serine/threonine-protein kinase isoform X8
XM_030243284.1	XP_030099144.1	striated muscle-specific serine/threonine-protein kinase isoform X9
XM_006496401.2	XP_006496464.1	striated muscle-specific serine/threonine-protein kinase isoform X9
XM_006496402.2	XP_006496465.1	striated muscle-specific serine/threonine-protein kinase isoform X10
NM_001173477.1	NP_001166948.1	striated muscle-specific serine/threonine-protein kinase isoform 4
XM_017312276.1	XP_017167765.1	striated muscle-specific serine/threonine-protein kinase isoform X13
XM_011238646.1	XP_011236948.1	striated muscle-specific serine/threonine-protein kinase isoform X5
XM_017322375.1	XP_017177864.1	striated muscle-specific serine/threonine-protein kinase isoform X6
XM_011238649.2	XP_011236951.1	striated muscle-specific serine/threonine-protein kinase isoform X8
XM_011238649.3	XP_011236951.1	striated muscle-specific serine/threonine-protein kinase isoform X9
XM_030243368.1	XP_030099228.1	striated muscle-specific serine/threonine-protein kinase isoform X14
XM_030243402.1	XP_030099262.1	striated muscle-specific serine/threonine-protein kinase isoform X16
XM_030243521.1	XP_030099381.1	striated muscle-specific serine/threonine-protein kinase isoform X19
NM_001085371.1	NP_001078840.1	striated muscle-specific serine/threonine-protein kinase isoform 3
NM_001085370.1	NP_001078839.1	striated muscle-specific serine/threonine-protein kinase isoform 2
XM_011238650.2	XP_011236952.1	striated muscle-specific serine/threonine-protein kinase isoform X11
XM_011238643.1	XP_011236945.1	striated muscle-specific serine/threonine-protein kinase isoform X1
XM_011238647.1	XP_011236949.1	striated muscle-specific serine/threonine-protein kinase isoform X7
XM_011238646.2	XP_011236948.1	striated muscle-specific serine/threonine-protein kinase isoform X6
XM_017322375.2	XP_017177864.1	striated muscle-specific serine/threonine-protein kinase isoform X7
XM_011238651.2	XP_011236953.1	striated muscle-specific serine/threonine-protein kinase isoform X13
XM_030243476.1	XP_030099336.1	striated muscle-specific serine/threonine-protein kinase isoform X19
XM_030243494.1	XP_030099354.1	striated muscle-specific serine/threonine-protein kinase isoform X19
XM_011238645.1	XP_011236947.1	striated muscle-specific serine/threonine-protein kinase isoform X4
XM_006496403.3	XP_006496466.1	striated muscle-specific serine/threonine-protein kinase isoform X10
XM_011238643.2	XP_011236945.1	striated muscle-specific serine/threonine-protein kinase isoform X1
XM_011238644.2	XP_011236946.1	striated muscle-specific serine/threonine-protein kinase isoform X3
XM_017322354.2	XP_017177843.1	striated muscle-specific serine/threonine-protein kinase isoform X4
XM_011238645.2	XP_011236947.1	striated muscle-specific serine/threonine-protein kinase isoform X5
XM_011238648.3	XP_011236950.1	striated muscle-specific serine/threonine-protein kinase isoform X9
XM_030243273.1	XP_030099133.1	striated muscle-specific serine/threonine-protein kinase isoform X9
XM_006496403.4	XP_006496466.1	striated muscle-specific serine/threonine-protein kinase isoform X11
XM_017312276.2	XP_017167765.1	striated muscle-specific serine/threonine-protein kinase isoform X17
XM_030243447.1	XP_030099307.1	striated muscle-specific serine/threonine-protein kinase isoform X18

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SPEG	NP_001135957.1
Mus musculus (house mouse)	Speg	NP_031489.4
Rattus norvegicus (Norway rat)	Speg	NP_001102272.1
Gallus gallus (chicken)	SPEG	XP_003641690.2
Danio rerio (zebrafish)	si:ch211-195m20.1	XP_691634.6
Homo sapiens (human)	SPEG	NP_005867.3
Pan troglodytes (chimpanzee)	SPEG	NP_001166952.1
	SPEG	XP_001104264.2
Canis lupus familiaris (dog)	SPEG	NP_001166949.1
Xenopus tropicalis (tropical clawed frog)	speg	XP_002934009.2

Gene Ontology
Bibliography

Related articles in PubMed

SPEG (Striated Muscle Preferentially Expressed Protein Kinase) Is Essential for Cardiac Function by Regulating Junctional Membrane Complex Activity.
Quick AP, Wang Q, Philippen LE, Barreto-Torres G, Chiang DY, Beavers D, Wang G, Khalid M, Reynolds JO, Campbell HM, Showell J, McCauley MD, Scholten A, Wehrens XH
Circulation research120(1)110-119(2017 Jan)

Disruption of striated preferentially expressed gene locus leads to dilated cardiomyopathy in mice.
Liu X, Ramjiganesh T, Chen YH, Chung SW, Hall SR, Schissel SL, Padera RF Jr, Liao R, Ackerman KG, Kajstura J, Leri A, Anversa P, Yet SF, Layne MD, Perrella MA
Circulation119(2)261-8(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Acute loss of SPEG in hearts causes junctophilin-2 de-phosphorylation leading to heart failure.
Title: SPEG (Striated Muscle Preferentially Expressed Protein Kinase) Is Essential for Cardiac Function by Regulating Junctional Membrane Complex Activity.

Disruption of the striated preferentially expressed gene (Speg) locus leads to cardiac dysfunction and a phenotype consistent with dilated cardiomyopathy.
Title: Disruption of striated preferentially expressed gene locus leads to dilated cardiomyopathy in mice.

The following Speg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Speg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00123	NM_001173477.1 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu84990	XM_030243512.1 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu84990	XM_030243494.1 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu84990	XM_030243521.1 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu84990	XM_030243476.1 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu02915	NM_001085371.1 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu67150	XM_011238651.2 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu76946	XM_017312276.2 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu84986	XM_030243368.1 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu84987	XM_030243390.1 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu84988	XM_030243402.1 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu84989	XM_030243447.1 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu84985	XM_030243348.1 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu67149	XM_011238650.2 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu20941	XM_030243284.1 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu20941	XM_011238648.3 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu20941	XM_030243273.1 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu20941	XM_011238649.3 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu27706	XM_006496404.4 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu27706	XM_006496403.4 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu20941	NM_001085370.1 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu20941	XM_006496400.2 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu20941	XM_006496401.2 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu27705	XM_006496402.2 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu00217	NM_007463.4 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu67144	XM_011238643.2 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu67145	XM_011238644.2 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu67146	XM_011238645.2 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu67147	XM_011238646.2 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu67148	XM_011238647.2 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu76944	XM_017322354.2 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu76945	XM_017322375.2 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu84984	XM_030243202.1 Latest version!	Mus musculus SPEG complex locus (Speg), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OMu27706	XM_006496404.3	Mus musculus SPEG complex locus (Speg), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu20941	XM_011238649.2	Mus musculus SPEG complex locus (Speg), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu67148	XM_011238647.1	Mus musculus SPEG complex locus (Speg), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu67147	XM_011238646.1	Mus musculus SPEG complex locus (Speg), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu76945	XM_017322375.1	Mus musculus SPEG complex locus (Speg), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu67144	XM_011238643.1	Mus musculus SPEG complex locus (Speg), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu67145	XM_011238644.1	Mus musculus SPEG complex locus (Speg), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu76944	XM_017322354.1	Mus musculus SPEG complex locus (Speg), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu67146	XM_011238645.1	Mus musculus SPEG complex locus (Speg), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu20941	XM_011238648.2	Mus musculus SPEG complex locus (Speg), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu27706	XM_006496403.3	Mus musculus SPEG complex locus (Speg), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu76946	XM_017312276.1	Mus musculus SPEG complex locus (Speg), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Speg ( NM_001173477.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001166948.1 Mus musculus SPEG complex locus (Speg), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu00123
Clone ID Related Accession (Same CDS sequence)	NM_001173477.1
Accession Version	NM_001173477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY034731.1, CK622939.1, AK164360.1 and BX526543.1. Transcript Variant: This variant (4) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon, compared to variant 1. The encoded protein (isoform 4) is shorter than isoform 1. This variant corresponds to variant APEG-1 described in PubMed ID: 10973969. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CX241322.1, CV560840.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCTCA GTCCCAGCCA GGATCATGAT TCCTCCGACT CTTCTTCCAA GGCACCCCCA 
ACGTTCAAGG TCTCACTCAT GGACCAATCG GTGAGAGAAG GTCAAGATGT CATTATGAGC 
ATCCGTGTGC AGGGAGAGCC CAAGCCTGTG GTTTCCTGGC TGAGGAATCG ACAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCAGAGGAG GCCGAGGGTG GGCTCTGCCG CTTGAGGATC 
CTGGCTGCTG AACGGGGCGA TGCTGGTTTC TACACATGCA AGGCGGTCAA CGAATATGGC 
GCTCGGCAGT GCGAGGCCCG CCTGGAGGTC CGAGGCGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166948.1
CDS	286..627
Translation	MKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	NM_001173477.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001173477.1

ORF Insert Sequence:

ATGAAGCTCA GTCCCAGCCA GGATCATGAT TCCTCCGACT CTTCTTCCAA GGCACCCCCA 
ACGTTCAAGG TCTCACTCAT GGACCAATCG GTGAGAGAAG GTCAAGATGT CATTATGAGC 
ATCCGTGTGC AGGGAGAGCC CAAGCCTGTG GTTTCCTGGC TGAGGAATCG ACAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCAGAGGAG GCCGAGGGTG GGCTCTGCCG CTTGAGGATC 
CTGGCTGCTG AACGGGGCGA TGCTGGTTTC TACACATGCA AGGCGGTCAA CGAATATGGC 
GCTCGGCAGT GCGAGGCCCG CCTGGAGGTC CGAGGCGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_030243512.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099372.1 Mus musculus SPEG complex locus (Speg), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OMu84990
Clone ID Related Accession (Same CDS sequence)	XM_030243512.1 , XM_030243521.1 , XM_030243476.1 , XM_030243494.1
Accession Version	XM_030243512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099372.1
CDS	2998..3696
Translation	MVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	XM_030243512.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243512.1

ORF Insert Sequence:

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_030243521.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099381.1 Mus musculus SPEG complex locus (Speg), transcript variant X28, mRNA.

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CloneID	OMu84990
Clone ID Related Accession (Same CDS sequence)	XM_030243512.1 , XM_030243521.1 , XM_030243476.1 , XM_030243494.1
Accession Version	XM_030243521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099381.1
CDS	421..1119
Translation	MVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	XM_030243521.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243521.1

ORF Insert Sequence:

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_030243476.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099336.1 Mus musculus SPEG complex locus (Speg), transcript variant X25, mRNA.

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CloneID	OMu84990
Clone ID Related Accession (Same CDS sequence)	XM_030243512.1 , XM_030243521.1 , XM_030243476.1 , XM_030243494.1
Accession Version	XM_030243476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099336.1
CDS	1748..2446
Translation	MVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	XM_030243476.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243476.1

ORF Insert Sequence:

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_030243494.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099354.1 Mus musculus SPEG complex locus (Speg), transcript variant X26, mRNA.

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CloneID	OMu84990
Clone ID Related Accession (Same CDS sequence)	XM_030243512.1 , XM_030243521.1 , XM_030243476.1 , XM_030243494.1
Accession Version	XM_030243494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099354.1
CDS	2088..2786
Translation	MVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	XM_030243494.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243494.1

ORF Insert Sequence:

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( NM_001085371.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001078840.1 Mus musculus SPEG complex locus (Speg), transcript variant 3, mRNA.

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CloneID	OMu02915
Clone ID Related Accession (Same CDS sequence)	NM_001085371.1
Accession Version	NM_001085371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY347209.1, AK164360.1 and BX526543.1. Transcript Variant: This variant (3) differs in the 5' UTR, 3' UTR, and coding region, compared to variant 1. The resulting protein (isoform 3) maintains the reading frame but is shorter at both the N- and C-termini when it is compared to isoform 1. This variant corresponds to variant BPEG described in PubMed ID: 10973969. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK164360.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGACTC GGAGACTGCC GAGGATGACA TCAGCGATGT GCCGGGGACC 
CAGCGCCTGG AGCTTCGGGA TGACAGGGCC TTCAGCACCC CCACGGGGGG CTCTGATACC 
CTGGTGGGTA CCTCCCTGGA CACACCCCCG ACCTCCGTGA CAGGCACCTC AGAGGAGCAA 
GTGAGCTGGT GGGGCAGCGG GCAGACGGTC CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC 
ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA GCACAGACAA CCGGGGCCGG GCCACGGCAC 
CTGGGGGTGG AGCCGCTGGT GCGGGCATCG CGAGCTAATC TGGTGGGCGC AAGCTGGGGG 
TCAGAGGATA GCCTTTCGGT GGCCAGTGAC CTCTATGGCA GCGCGTTCAG TCTGTACAGA 
GGACGGGCGC TCTCGATCCA CGTCAGCATT CCTCCGAGTG GATTGCACAG AGAGGAGCCT 
GACCTTCAGC CTCAGCCAGC CAGTGATGCC CTTCGTCCAC GCCCTGCCCT TCCACCTCCC 
TCCAAATCCG CTTTGCTTCC CCCACCATCT CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA 
CCTAGTACCC AGCCCCCAGC TACTCCCACT TCGCCCCATC GGCGTGCTCA GGAGCCCTCG 
CTGCCTGAGG ACATCACCAC CACGGAAGAG AAGCGAGGGA AAAAGCCCAA GTCATCCGGG 
CCGTCGCTGG CGGGCACAGT GGAGTCCCGG CCCCAGACGC CACTGAGCGA GGCTTCGGGT 
CGCCTGTCAG CATTGGGCCG CTCGCCCCGG CTGGTGCGCG CGGGGTCCCG CATCCTGGAC 
AAGCTACAGT TCTTCGAAGA GCGGCGACGC AGCCTGGAGC GCAGCGACTC GCCGCCAGCG 
CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG 
GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC 
AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG CAAAAGGCGG CCTCGTTGGA TGAACGCACG 
CGACAACGCA GTGCAACCTC GGACCTCGAA CTCCGCTTCG CCCAGGAGCT GGGTCGCATC 
CGCCGATCTA CGTCGCGGGA GGAGCTGGTG CGTTCGCACG AGTCCCTGCG TGCCACGCTG 
CAGCGCGCCC CATCCCCTCG GGAGCCCGGC GAGCCCCCAC TCTTCTCCCG GCCTTCCACA 
CCCAAGACCT CACGGGCTGT GAGCCCGGCT GCCACCCAGC CGCCGCCTCC TAGTGGTGCG 
GGCAAATCTG GGGACGAGCC TGGGAGGCCC CGAAGCAGAG GGCCGGTGGG CAGGACTGAA 
CCGGGGGAAG GCCCGCAGCA GGAGATCAAG CGTCGGGACC AATTCCCGCT AACCAGGAGC 
AGAGCCATCC AGGAGTGCAG GAGCCCTGTG CCGCCCTACA CCGCGGATCC CCCGGAGAGC 
AGGACAAAAG CCCCCTCCGG TCGCAAGCGG GAACCCCCTG CTCAAGCGGT GCGCTTTCTG 
CCCTGGGCCA CTCCGGGAGT GGAGGACTCT GTTCTGCCCC AAACCTTGGA GAAGAATAGA 
GCGGGACCCG AGGCTGAGAA GAGGCTTCGC AGAGGACCTG AGGAGGATGG CCCCTGGGGG 
CCCTGGGACC GCAGAGGGAC CCGCAGCCAA GGCAAAGGTC GCCGTGCTCG GCCTACTTCC 
CCCGAGCTCG AGTCCTCAGA CGACTCCTAT GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA 
CCCGTGTTTG AGATCCCTCT GCAGAATATG GTGGTGGCGC CAGGAGCTGA CGTGCTACTT 
AAGTGTATCA TCACCGCCAA CCCCCCACCC CAAGTGTCCT GGAAAAAGGA TGGGTCCATG 
TTGCACAGCG AGGGTCGTCT TCTCATCCGG GCTGAAGGTG AACGGCACAC ACTGCTGCTC 
AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC TACACAGCCA CTGCCACCAA CGAACTGGGC 
CAAGCTACCT GTGCTTCTTC ACTGGCTGTG AGACCTGGCG GCTCCACATC CCCTTTCAGC 
AGCCCCATCA CCTCTGATGA GGAGTACCTG AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG 
GAGACCTGGC CCCGAACCCC TACCATGAAG CTCAGTCCCA GCCAGGATCA TGATTCCTCC 
GACTCTTCTT CCAAGGCACC CCCAACGTTC AAGGTCTCAC TCATGGACCA ATCGGTGAGA 
GAAGGTCAAG ATGTCATTAT GAGCATCCGT GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC 
TGGCTGAGGA ATCGACAGCC CGTGCGCCCA GACCAGCGGC GCTTTGCAGA GGAGGCCGAG 
GGTGGGCTCT GCCGCTTGAG GATCCTGGCT GCTGAACGGG GCGATGCTGG TTTCTACACA 
TGCAAGGCGG TCAACGAATA TGGCGCTCGG CAGTGCGAGG CCCGCCTGGA GGTCCGAGGC 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078840.1
CDS	29..2614
Translation	MKKLWVKKRFQKTGHSRRAFGRLTHDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPRQAQTTGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	NM_001085371.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001085371.1

ORF Insert Sequence:

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGACTC GGAGACTGCC GAGGATGACA TCAGCGATGT GCCGGGGACC 
CAGCGCCTGG AGCTTCGGGA TGACAGGGCC TTCAGCACCC CCACGGGGGG CTCTGATACC 
CTGGTGGGTA CCTCCCTGGA CACACCCCCG ACCTCCGTGA CAGGCACCTC AGAGGAGCAA 
GTGAGCTGGT GGGGCAGCGG GCAGACGGTC CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC 
ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA GCACAGACAA CCGGGGCCGG GCCACGGCAC 
CTGGGGGTGG AGCCGCTGGT GCGGGCATCG CGAGCTAATC TGGTGGGCGC AAGCTGGGGG 
TCAGAGGATA GCCTTTCGGT GGCCAGTGAC CTCTATGGCA GCGCGTTCAG TCTGTACAGA 
GGACGGGCGC TCTCGATCCA CGTCAGCATT CCTCCGAGTG GATTGCACAG AGAGGAGCCT 
GACCTTCAGC CTCAGCCAGC CAGTGATGCC CTTCGTCCAC GCCCTGCCCT TCCACCTCCC 
TCCAAATCCG CTTTGCTTCC CCCACCATCT CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA 
CCTAGTACCC AGCCCCCAGC TACTCCCACT TCGCCCCATC GGCGTGCTCA GGAGCCCTCG 
CTGCCTGAGG ACATCACCAC CACGGAAGAG AAGCGAGGGA AAAAGCCCAA GTCATCCGGG 
CCGTCGCTGG CGGGCACAGT GGAGTCCCGG CCCCAGACGC CACTGAGCGA GGCTTCGGGT 
CGCCTGTCAG CATTGGGCCG CTCGCCCCGG CTGGTGCGCG CGGGGTCCCG CATCCTGGAC 
AAGCTACAGT TCTTCGAAGA GCGGCGACGC AGCCTGGAGC GCAGCGACTC GCCGCCAGCG 
CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG 
GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC 
AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG CAAAAGGCGG CCTCGTTGGA TGAACGCACG 
CGACAACGCA GTGCAACCTC GGACCTCGAA CTCCGCTTCG CCCAGGAGCT GGGTCGCATC 
CGCCGATCTA CGTCGCGGGA GGAGCTGGTG CGTTCGCACG AGTCCCTGCG TGCCACGCTG 
CAGCGCGCCC CATCCCCTCG GGAGCCCGGC GAGCCCCCAC TCTTCTCCCG GCCTTCCACA 
CCCAAGACCT CACGGGCTGT GAGCCCGGCT GCCACCCAGC CGCCGCCTCC TAGTGGTGCG 
GGCAAATCTG GGGACGAGCC TGGGAGGCCC CGAAGCAGAG GGCCGGTGGG CAGGACTGAA 
CCGGGGGAAG GCCCGCAGCA GGAGATCAAG CGTCGGGACC AATTCCCGCT AACCAGGAGC 
AGAGCCATCC AGGAGTGCAG GAGCCCTGTG CCGCCCTACA CCGCGGATCC CCCGGAGAGC 
AGGACAAAAG CCCCCTCCGG TCGCAAGCGG GAACCCCCTG CTCAAGCGGT GCGCTTTCTG 
CCCTGGGCCA CTCCGGGAGT GGAGGACTCT GTTCTGCCCC AAACCTTGGA GAAGAATAGA 
GCGGGACCCG AGGCTGAGAA GAGGCTTCGC AGAGGACCTG AGGAGGATGG CCCCTGGGGG 
CCCTGGGACC GCAGAGGGAC CCGCAGCCAA GGCAAAGGTC GCCGTGCTCG GCCTACTTCC 
CCCGAGCTCG AGTCCTCAGA CGACTCCTAT GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA 
CCCGTGTTTG AGATCCCTCT GCAGAATATG GTGGTGGCGC CAGGAGCTGA CGTGCTACTT 
AAGTGTATCA TCACCGCCAA CCCCCCACCC CAAGTGTCCT GGAAAAAGGA TGGGTCCATG 
TTGCACAGCG AGGGTCGTCT TCTCATCCGG GCTGAAGGTG AACGGCACAC ACTGCTGCTC 
AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC TACACAGCCA CTGCCACCAA CGAACTGGGC 
CAAGCTACCT GTGCTTCTTC ACTGGCTGTG AGACCTGGCG GCTCCACATC CCCTTTCAGC 
AGCCCCATCA CCTCTGATGA GGAGTACCTG AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG 
GAGACCTGGC CCCGAACCCC TACCATGAAG CTCAGTCCCA GCCAGGATCA TGATTCCTCC 
GACTCTTCTT CCAAGGCACC CCCAACGTTC AAGGTCTCAC TCATGGACCA ATCGGTGAGA 
GAAGGTCAAG ATGTCATTAT GAGCATCCGT GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC 
TGGCTGAGGA ATCGACAGCC CGTGCGCCCA GACCAGCGGC GCTTTGCAGA GGAGGCCGAG 
GGTGGGCTCT GCCGCTTGAG GATCCTGGCT GCTGAACGGG GCGATGCTGG TTTCTACACA 
TGCAAGGCGG TCAACGAATA TGGCGCTCGG CAGTGCGAGG CCCGCCTGGA GGTCCGAGGC 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_011238651.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236953.1 Mus musculus SPEG complex locus (Speg), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OMu67150
Clone ID Related Accession (Same CDS sequence)	XM_011238651.2
Accession Version	XM_011238651.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238651.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
TACGACTCGG AGACTGCCGA GGATGACATC AGCGATGTGC CGGGGACCCA GCGCCTGGAG 
CTTCGGGATG ACAGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGTACC 
TCCCTGGACA CACCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG 
GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC 
CCAGGCAGCC CAAGGCAAGC ACAGACAACC GGGGCCGGGC CACGGCACCT GGGGGTGGAG 
CCGCTGGTGC GGGCATCGCG AGCTAATCTG GTGGGCGCAA GCTGGGGGTC AGAGGATAGC 
CTTTCGGTGG CCAGTGACCT CTATGGCAGC GCGTTCAGTC TGTACAGAGG ACGGGCGCTC 
TCGATCCACG TCAGCATTCC TCCGAGTGGA TTGCACAGAG AGGAGCCTGA CCTTCAGCCT 
CAGCCAGCCA GTGATGCCCT TCGTCCACGC CCTGCCCTTC CACCTCCCTC CAAATCCGCT 
TTGCTTCCCC CACCATCTCC TCGGGTGGGA AAGAGGGCTC TGCCAGGACC TAGTACCCAG 
CCCCCAGCTA CTCCCACTTC GCCCCATCGG CGTGCTCAGG AGCCCTCGCT GCCTGAGGAC 
ATCACCACCA CGGAAGAGAA GCGAGGGAAA AAGCCCAAGT CATCCGGGCC GTCGCTGGCG 
GGCACAGTGG AGTCCCGGCC CCAGACGCCA CTGAGCGAGG CTTCGGGTCG CCTGTCAGCA 
TTGGGCCGCT CGCCCCGGCT GGTGCGCGCG GGGTCCCGCA TCCTGGACAA GCTACAGTTC 
TTCGAAGAGC GGCGACGCAG CCTGGAGCGC AGCGACTCGC CGCCAGCGCC CCTGCGGCCC 
TGGGTGCCCC TGCGCAAGGC TCGCTCGCTG GAGCAGCCGA AGTCCGAGGG CGGTGCGGCG 
TGGGGCACAC CCGAGGCCTC GCAGGAGGAG CTGCGGTCAC CTCGGGGCAG TGTGGCAGAG 
CGGCGTCGCC TGTTCCAGCA AAAGGCGGCC TCGTTGGATG AACGCACGCG ACAACGCAGT 
GCAACCTCGG ACCTCGAACT CCGCTTCGCC CAGGAGCTGG GTCGCATCCG CCGATCTACG 
TCGCGGGAGG AGCTGGTGCG TTCGCACGAG TCCCTGCGTG CCACGCTGCA GCGCGCCCCA 
TCCCCTCGGG AGCCCGGCGA GCCCCCACTC TTCTCCCGGC CTTCCACACC CAAGACCTCA 
CGGGCTGTGA GCCCGGCTGC CACCCAGCCG CCGCCTCCTA GTGGTGCGGG CAAATCTGGG 
GACGAGCCTG GGAGGCCCCG AAGCAGAGGG CCGGTGGGCA GGACTGAACC GGGGGAAGGC 
CCGCAGCAGG AGATCAAGCG TCGGGACCAA TTCCCGCTAA CCAGGAGCAG AGCCATCCAG 
GAGTGCAGGA GCCCTGTGCC GCCCTACACC GCGGATCCCC CGGAGAGCAG GACAAAAGCC 
CCCTCCGGTC GCAAGCGGGA ACCCCCTGCT CAAGCGGTGC GCTTTCTGCC CTGGGCCACT 
CCGGGAGTGG AGGACTCTGT TCTGCCCCAA ACCTTGGAGA AGAATAGAGC GGGACCCGAG 
GCTGAGAAGA GGCTTCGCAG AGGACCTGAG GAGGATGGCC CCTGGGGGCC CTGGGACCGC 
AGAGGGACCC GCAGCCAAGG CAAAGGTCGC CGTGCTCGGC CTACTTCCCC CGAGCTCGAG 
TCCTCAGACG ACTCCTATGT GTCCGCTGGG GAAGAGCCCC TGGAGGCACC CGTGTTTGAG 
ATCCCTCTGC AGAATATGGT GGTGGCGCCA GGAGCTGACG TGCTACTTAA GTGTATCATC 
ACCGCCAACC CCCCACCCCA AGTGTCCTGG AAAAAGGATG GGTCCATGTT GCACAGCGAG 
GGTCGTCTTC TCATCCGGGC TGAAGGTGAA CGGCACACAC TGCTGCTCAG AGAGGCCCAG 
GCTGCCGATG CTGGGAGCTA CACAGCCACT GCCACCAACG AACTGGGCCA AGCTACCTGT 
GCTTCTTCAC TGGCTGTGAG ACCTGGCGGC TCCACATCCC CTTTCAGCAG CCCCATCACC 
TCTGATGAGG AGTACCTGAG CCCCCCAGAG GAGTTCCCAG AGCCTGGGGA GACCTGGCCC 
CGAACCCCTA CCATGAAGCT CAGTCCCAGC CAGGATCATG ATTCCTCCGA CTCTTCTTCC 
AAGGCACCCC CAACGTTCAA GGTCTCACTC ATGGACCAAT CGGTGAGAGA AGGTCAAGAT 
GTCATTATGA GCATCCGTGT GCAGGGAGAG CCCAAGCCTG TGGTTTCCTG GCTGAGGAAT 
CGACAGCCCG TGCGCCCAGA CCAGCGGCGC TTTGCAGAGG AGGCCGAGGG TGGGCTCTGC 
CGCTTGAGGA TCCTGGCTGC TGAACGGGGC GATGCTGGTT TCTACACATG CAAGGCGGTC 
AACGAATATG GCGCTCGGCA GTGCGAGGCC CGCCTGGAGG TCCGAGGCGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236953.1
CDS	80..3013
Translation	MQKARGTRGEDAGTRAPPSPGVPPKRAKVGAGRGVLVTGDGAGAPVFLRPLKNAAVCAGSDVRLRVVVSGTPQPSLSWFRDGQLLPPPAPEPSCLWLRSCGAQDAGVYSCSAQNERGQASCEAVLTVLEVRVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPRQAQTTGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	XM_011238651.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238651.2

ORF Insert Sequence:

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
TACGACTCGG AGACTGCCGA GGATGACATC AGCGATGTGC CGGGGACCCA GCGCCTGGAG 
CTTCGGGATG ACAGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGTACC 
TCCCTGGACA CACCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG 
GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC 
CCAGGCAGCC CAAGGCAAGC ACAGACAACC GGGGCCGGGC CACGGCACCT GGGGGTGGAG 
CCGCTGGTGC GGGCATCGCG AGCTAATCTG GTGGGCGCAA GCTGGGGGTC AGAGGATAGC 
CTTTCGGTGG CCAGTGACCT CTATGGCAGC GCGTTCAGTC TGTACAGAGG ACGGGCGCTC 
TCGATCCACG TCAGCATTCC TCCGAGTGGA TTGCACAGAG AGGAGCCTGA CCTTCAGCCT 
CAGCCAGCCA GTGATGCCCT TCGTCCACGC CCTGCCCTTC CACCTCCCTC CAAATCCGCT 
TTGCTTCCCC CACCATCTCC TCGGGTGGGA AAGAGGGCTC TGCCAGGACC TAGTACCCAG 
CCCCCAGCTA CTCCCACTTC GCCCCATCGG CGTGCTCAGG AGCCCTCGCT GCCTGAGGAC 
ATCACCACCA CGGAAGAGAA GCGAGGGAAA AAGCCCAAGT CATCCGGGCC GTCGCTGGCG 
GGCACAGTGG AGTCCCGGCC CCAGACGCCA CTGAGCGAGG CTTCGGGTCG CCTGTCAGCA 
TTGGGCCGCT CGCCCCGGCT GGTGCGCGCG GGGTCCCGCA TCCTGGACAA GCTACAGTTC 
TTCGAAGAGC GGCGACGCAG CCTGGAGCGC AGCGACTCGC CGCCAGCGCC CCTGCGGCCC 
TGGGTGCCCC TGCGCAAGGC TCGCTCGCTG GAGCAGCCGA AGTCCGAGGG CGGTGCGGCG 
TGGGGCACAC CCGAGGCCTC GCAGGAGGAG CTGCGGTCAC CTCGGGGCAG TGTGGCAGAG 
CGGCGTCGCC TGTTCCAGCA AAAGGCGGCC TCGTTGGATG AACGCACGCG ACAACGCAGT 
GCAACCTCGG ACCTCGAACT CCGCTTCGCC CAGGAGCTGG GTCGCATCCG CCGATCTACG 
TCGCGGGAGG AGCTGGTGCG TTCGCACGAG TCCCTGCGTG CCACGCTGCA GCGCGCCCCA 
TCCCCTCGGG AGCCCGGCGA GCCCCCACTC TTCTCCCGGC CTTCCACACC CAAGACCTCA 
CGGGCTGTGA GCCCGGCTGC CACCCAGCCG CCGCCTCCTA GTGGTGCGGG CAAATCTGGG 
GACGAGCCTG GGAGGCCCCG AAGCAGAGGG CCGGTGGGCA GGACTGAACC GGGGGAAGGC 
CCGCAGCAGG AGATCAAGCG TCGGGACCAA TTCCCGCTAA CCAGGAGCAG AGCCATCCAG 
GAGTGCAGGA GCCCTGTGCC GCCCTACACC GCGGATCCCC CGGAGAGCAG GACAAAAGCC 
CCCTCCGGTC GCAAGCGGGA ACCCCCTGCT CAAGCGGTGC GCTTTCTGCC CTGGGCCACT 
CCGGGAGTGG AGGACTCTGT TCTGCCCCAA ACCTTGGAGA AGAATAGAGC GGGACCCGAG 
GCTGAGAAGA GGCTTCGCAG AGGACCTGAG GAGGATGGCC CCTGGGGGCC CTGGGACCGC 
AGAGGGACCC GCAGCCAAGG CAAAGGTCGC CGTGCTCGGC CTACTTCCCC CGAGCTCGAG 
TCCTCAGACG ACTCCTATGT GTCCGCTGGG GAAGAGCCCC TGGAGGCACC CGTGTTTGAG 
ATCCCTCTGC AGAATATGGT GGTGGCGCCA GGAGCTGACG TGCTACTTAA GTGTATCATC 
ACCGCCAACC CCCCACCCCA AGTGTCCTGG AAAAAGGATG GGTCCATGTT GCACAGCGAG 
GGTCGTCTTC TCATCCGGGC TGAAGGTGAA CGGCACACAC TGCTGCTCAG AGAGGCCCAG 
GCTGCCGATG CTGGGAGCTA CACAGCCACT GCCACCAACG AACTGGGCCA AGCTACCTGT 
GCTTCTTCAC TGGCTGTGAG ACCTGGCGGC TCCACATCCC CTTTCAGCAG CCCCATCACC 
TCTGATGAGG AGTACCTGAG CCCCCCAGAG GAGTTCCCAG AGCCTGGGGA GACCTGGCCC 
CGAACCCCTA CCATGAAGCT CAGTCCCAGC CAGGATCATG ATTCCTCCGA CTCTTCTTCC 
AAGGCACCCC CAACGTTCAA GGTCTCACTC ATGGACCAAT CGGTGAGAGA AGGTCAAGAT 
GTCATTATGA GCATCCGTGT GCAGGGAGAG CCCAAGCCTG TGGTTTCCTG GCTGAGGAAT 
CGACAGCCCG TGCGCCCAGA CCAGCGGCGC TTTGCAGAGG AGGCCGAGGG TGGGCTCTGC 
CGCTTGAGGA TCCTGGCTGC TGAACGGGGC GATGCTGGTT TCTACACATG CAAGGCGGTC 
AACGAATATG GCGCTCGGCA GTGCGAGGCC CGCCTGGAGG TCCGAGGCGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_017312276.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017167765.1 Mus musculus SPEG complex locus (Speg), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OMu76946
Clone ID Related Accession (Same CDS sequence)	XM_017312276.1 , XM_017312276.2
Accession Version	XM_017312276.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACC AGTGTTGGTA CCTTCTGGGG AGGGGGTCTC CCTCTCCTCC CCATCTCACA 
GAACATCTCT GGTATCCCTG GACAGCAGGG ACCCTCATGC CTCCCTATAT TCTGCCTGCC 
TTCCATGCCC CATCCCACAA ACACTCTGAT AACTGTCTGT CCACATCTCT CTCCTGCTGC 
TCCTATGGAA GCGAAGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGCAGC 
ATTCCTCCGA GTGGATTGCA CAGAGAGGAG CCTGACCTTC AGCCTCAGCC AGCCAGTGAT 
GCCCTTCGTC CACGCCCTGC CCTTCCACCT CCCTCCAAAT CCGCTTTGCT TCCCCCACCA 
TCTCCTCGGG TGGGAAAGAG GGCTCTGCCA GGACCTAGTA CCCAGCCCCC AGCTACTCCC 
ACTTCGCCCC ATCGGCGTGC TCAGGAGCCC TCGCTGCCTG AGGACATCAC CACCACGGAA 
GAGAAGCGAG GGAAAAAGCC CAAGTCATCC GGGCCGTCGC TGGCGGGCAC AGTGGAGTCC 
CGGCCCCAGA CGCCACTGAG CGAGGCTTCG GGTCGCCTGT CAGCATTGGG CCGCTCGCCC 
CGGCTGGTGC GCGCGGGGTC CCGCATCCTG GACAAGCTAC AGTTCTTCGA AGAGCGGCGA 
CGCAGCCTGG AGCGCAGCGA CTCGCCGCCA GCGCCCCTGC GGCCCTGGGT GCCCCTGCGC 
AAGGCTCGCT CGCTGGAGCA GCCGAAGTCC GAGGGCGGTG CGGCGTGGGG CACACCCGAG 
GCCTCGCAGG AGGAGCTGCG GTCACCTCGG GGCAGTGTGG CAGAGCGGCG TCGCCTGTTC 
CAGCAAAAGG CGGCCTCGTT GGATGAACGC ACGCGACAAC GCAGTGCAAC CTCGGACCTC 
GAACTCCGCT TCGCCCAGGA GCTGGGTCGC ATCCGCCGAT CTACGTCGCG GGAGGAGCTG 
GTGCGTTCGC ACGAGTCCCT GCGTGCCACG CTGCAGCGCG CCCCATCCCC TCGGGAGCCC 
GGCGAGCCCC CACTCTTCTC CCGGCCTTCC ACACCCAAGA CCTCACGGGC TGTGAGCCCG 
GCTGCCACCC AGCCGCCGCC TCCTAGTGGT GCGGGCAAAT CTGGGGACGA GCCTGGGAGG 
CCCCGAAGCA GAGGGCCGGT GGGCAGGACT GAACCGGGGG AAGGCCCGCA GCAGGAGATC 
AAGCGTCGGG ACCAATTCCC GCTAACCAGG AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT 
GTGCCGCCCT ACACCGCGGA TCCCCCGGAG AGCAGGACAA AAGCCCCCTC CGGTCGCAAG 
CGGGAACCCC CTGCTCAAGC GGTGCGCTTT CTGCCCTGGG CCACTCCGGG AGTGGAGGAC 
TCTGTTCTGC CCCAAACCTT GGAGAAGAAT AGAGCGGGAC CCGAGGCTGA GAAGAGGCTT 
CGCAGAGGAC CTGAGGAGGA TGGCCCCTGG GGGCCCTGGG ACCGCAGAGG GACCCGCAGC 
CAAGGCAAAG GTCGCCGTGC TCGGCCTACT TCCCCCGAGC TCGAGTCCTC AGACGACTCC 
TATGTGTCCG CTGGGGAAGA GCCCCTGGAG GCACCCGTGT TTGAGATCCC TCTGCAGAAT 
ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017167765.1
CDS	261..2819
Translation	MAHQCWYLLGRGSPSPPHLTEHLWYPWTAGTLMPPYILPAFHAPSHKHSDNCLSTSLSCCSYGSEVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPSSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	XM_017312276.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312276.1

ORF Insert Sequence:

ATGGCTCACC AGTGTTGGTA CCTTCTGGGG AGGGGGTCTC CCTCTCCTCC CCATCTCACA 
GAACATCTCT GGTATCCCTG GACAGCAGGG ACCCTCATGC CTCCCTATAT TCTGCCTGCC 
TTCCATGCCC CATCCCACAA ACACTCTGAT AACTGTCTGT CCACATCTCT CTCCTGCTGC 
TCCTATGGAA GCGAAGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGCAGC 
ATTCCTCCGA GTGGATTGCA CAGAGAGGAG CCTGACCTTC AGCCTCAGCC AGCCAGTGAT 
GCCCTTCGTC CACGCCCTGC CCTTCCACCT CCCTCCAAAT CCGCTTTGCT TCCCCCACCA 
TCTCCTCGGG TGGGAAAGAG GGCTCTGCCA GGACCTAGTA CCCAGCCCCC AGCTACTCCC 
ACTTCGCCCC ATCGGCGTGC TCAGGAGCCC TCGCTGCCTG AGGACATCAC CACCACGGAA 
GAGAAGCGAG GGAAAAAGCC CAAGTCATCC GGGCCGTCGC TGGCGGGCAC AGTGGAGTCC 
CGGCCCCAGA CGCCACTGAG CGAGGCTTCG GGTCGCCTGT CAGCATTGGG CCGCTCGCCC 
CGGCTGGTGC GCGCGGGGTC CCGCATCCTG GACAAGCTAC AGTTCTTCGA AGAGCGGCGA 
CGCAGCCTGG AGCGCAGCGA CTCGCCGCCA GCGCCCCTGC GGCCCTGGGT GCCCCTGCGC 
AAGGCTCGCT CGCTGGAGCA GCCGAAGTCC GAGGGCGGTG CGGCGTGGGG CACACCCGAG 
GCCTCGCAGG AGGAGCTGCG GTCACCTCGG GGCAGTGTGG CAGAGCGGCG TCGCCTGTTC 
CAGCAAAAGG CGGCCTCGTT GGATGAACGC ACGCGACAAC GCAGTGCAAC CTCGGACCTC 
GAACTCCGCT TCGCCCAGGA GCTGGGTCGC ATCCGCCGAT CTACGTCGCG GGAGGAGCTG 
GTGCGTTCGC ACGAGTCCCT GCGTGCCACG CTGCAGCGCG CCCCATCCCC TCGGGAGCCC 
GGCGAGCCCC CACTCTTCTC CCGGCCTTCC ACACCCAAGA CCTCACGGGC TGTGAGCCCG 
GCTGCCACCC AGCCGCCGCC TCCTAGTGGT GCGGGCAAAT CTGGGGACGA GCCTGGGAGG 
CCCCGAAGCA GAGGGCCGGT GGGCAGGACT GAACCGGGGG AAGGCCCGCA GCAGGAGATC 
AAGCGTCGGG ACCAATTCCC GCTAACCAGG AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT 
GTGCCGCCCT ACACCGCGGA TCCCCCGGAG AGCAGGACAA AAGCCCCCTC CGGTCGCAAG 
CGGGAACCCC CTGCTCAAGC GGTGCGCTTT CTGCCCTGGG CCACTCCGGG AGTGGAGGAC 
TCTGTTCTGC CCCAAACCTT GGAGAAGAAT AGAGCGGGAC CCGAGGCTGA GAAGAGGCTT 
CGCAGAGGAC CTGAGGAGGA TGGCCCCTGG GGGCCCTGGG ACCGCAGAGG GACCCGCAGC 
CAAGGCAAAG GTCGCCGTGC TCGGCCTACT TCCCCCGAGC TCGAGTCCTC AGACGACTCC 
TATGTGTCCG CTGGGGAAGA GCCCCTGGAG GCACCCGTGT TTGAGATCCC TCTGCAGAAT 
ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_017312276.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017167765.1 Mus musculus SPEG complex locus (Speg), transcript variant X23, mRNA.

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CloneID	OMu76946
Clone ID Related Accession (Same CDS sequence)	XM_017312276.1 , XM_017312276.2
Accession Version	XM_017312276.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312276.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACC AGTGTTGGTA CCTTCTGGGG AGGGGGTCTC CCTCTCCTCC CCATCTCACA 
GAACATCTCT GGTATCCCTG GACAGCAGGG ACCCTCATGC CTCCCTATAT TCTGCCTGCC 
TTCCATGCCC CATCCCACAA ACACTCTGAT AACTGTCTGT CCACATCTCT CTCCTGCTGC 
TCCTATGGAA GCGAAGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGCAGC 
ATTCCTCCGA GTGGATTGCA CAGAGAGGAG CCTGACCTTC AGCCTCAGCC AGCCAGTGAT 
GCCCTTCGTC CACGCCCTGC CCTTCCACCT CCCTCCAAAT CCGCTTTGCT TCCCCCACCA 
TCTCCTCGGG TGGGAAAGAG GGCTCTGCCA GGACCTAGTA CCCAGCCCCC AGCTACTCCC 
ACTTCGCCCC ATCGGCGTGC TCAGGAGCCC TCGCTGCCTG AGGACATCAC CACCACGGAA 
GAGAAGCGAG GGAAAAAGCC CAAGTCATCC GGGCCGTCGC TGGCGGGCAC AGTGGAGTCC 
CGGCCCCAGA CGCCACTGAG CGAGGCTTCG GGTCGCCTGT CAGCATTGGG CCGCTCGCCC 
CGGCTGGTGC GCGCGGGGTC CCGCATCCTG GACAAGCTAC AGTTCTTCGA AGAGCGGCGA 
CGCAGCCTGG AGCGCAGCGA CTCGCCGCCA GCGCCCCTGC GGCCCTGGGT GCCCCTGCGC 
AAGGCTCGCT CGCTGGAGCA GCCGAAGTCC GAGGGCGGTG CGGCGTGGGG CACACCCGAG 
GCCTCGCAGG AGGAGCTGCG GTCACCTCGG GGCAGTGTGG CAGAGCGGCG TCGCCTGTTC 
CAGCAAAAGG CGGCCTCGTT GGATGAACGC ACGCGACAAC GCAGTGCAAC CTCGGACCTC 
GAACTCCGCT TCGCCCAGGA GCTGGGTCGC ATCCGCCGAT CTACGTCGCG GGAGGAGCTG 
GTGCGTTCGC ACGAGTCCCT GCGTGCCACG CTGCAGCGCG CCCCATCCCC TCGGGAGCCC 
GGCGAGCCCC CACTCTTCTC CCGGCCTTCC ACACCCAAGA CCTCACGGGC TGTGAGCCCG 
GCTGCCACCC AGCCGCCGCC TCCTAGTGGT GCGGGCAAAT CTGGGGACGA GCCTGGGAGG 
CCCCGAAGCA GAGGGCCGGT GGGCAGGACT GAACCGGGGG AAGGCCCGCA GCAGGAGATC 
AAGCGTCGGG ACCAATTCCC GCTAACCAGG AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT 
GTGCCGCCCT ACACCGCGGA TCCCCCGGAG AGCAGGACAA AAGCCCCCTC CGGTCGCAAG 
CGGGAACCCC CTGCTCAAGC GGTGCGCTTT CTGCCCTGGG CCACTCCGGG AGTGGAGGAC 
TCTGTTCTGC CCCAAACCTT GGAGAAGAAT AGAGCGGGAC CCGAGGCTGA GAAGAGGCTT 
CGCAGAGGAC CTGAGGAGGA TGGCCCCTGG GGGCCCTGGG ACCGCAGAGG GACCCGCAGC 
CAAGGCAAAG GTCGCCGTGC TCGGCCTACT TCCCCCGAGC TCGAGTCCTC AGACGACTCC 
TATGTGTCCG CTGGGGAAGA GCCCCTGGAG GCACCCGTGT TTGAGATCCC TCTGCAGAAT 
ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017167765.1
CDS	1992..4550
Translation	MAHQCWYLLGRGSPSPPHLTEHLWYPWTAGTLMPPYILPAFHAPSHKHSDNCLSTSLSCCSYGSEVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPSSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	XM_017312276.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312276.2

ORF Insert Sequence:

ATGGCTCACC AGTGTTGGTA CCTTCTGGGG AGGGGGTCTC CCTCTCCTCC CCATCTCACA 
GAACATCTCT GGTATCCCTG GACAGCAGGG ACCCTCATGC CTCCCTATAT TCTGCCTGCC 
TTCCATGCCC CATCCCACAA ACACTCTGAT AACTGTCTGT CCACATCTCT CTCCTGCTGC 
TCCTATGGAA GCGAAGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGCAGC 
ATTCCTCCGA GTGGATTGCA CAGAGAGGAG CCTGACCTTC AGCCTCAGCC AGCCAGTGAT 
GCCCTTCGTC CACGCCCTGC CCTTCCACCT CCCTCCAAAT CCGCTTTGCT TCCCCCACCA 
TCTCCTCGGG TGGGAAAGAG GGCTCTGCCA GGACCTAGTA CCCAGCCCCC AGCTACTCCC 
ACTTCGCCCC ATCGGCGTGC TCAGGAGCCC TCGCTGCCTG AGGACATCAC CACCACGGAA 
GAGAAGCGAG GGAAAAAGCC CAAGTCATCC GGGCCGTCGC TGGCGGGCAC AGTGGAGTCC 
CGGCCCCAGA CGCCACTGAG CGAGGCTTCG GGTCGCCTGT CAGCATTGGG CCGCTCGCCC 
CGGCTGGTGC GCGCGGGGTC CCGCATCCTG GACAAGCTAC AGTTCTTCGA AGAGCGGCGA 
CGCAGCCTGG AGCGCAGCGA CTCGCCGCCA GCGCCCCTGC GGCCCTGGGT GCCCCTGCGC 
AAGGCTCGCT CGCTGGAGCA GCCGAAGTCC GAGGGCGGTG CGGCGTGGGG CACACCCGAG 
GCCTCGCAGG AGGAGCTGCG GTCACCTCGG GGCAGTGTGG CAGAGCGGCG TCGCCTGTTC 
CAGCAAAAGG CGGCCTCGTT GGATGAACGC ACGCGACAAC GCAGTGCAAC CTCGGACCTC 
GAACTCCGCT TCGCCCAGGA GCTGGGTCGC ATCCGCCGAT CTACGTCGCG GGAGGAGCTG 
GTGCGTTCGC ACGAGTCCCT GCGTGCCACG CTGCAGCGCG CCCCATCCCC TCGGGAGCCC 
GGCGAGCCCC CACTCTTCTC CCGGCCTTCC ACACCCAAGA CCTCACGGGC TGTGAGCCCG 
GCTGCCACCC AGCCGCCGCC TCCTAGTGGT GCGGGCAAAT CTGGGGACGA GCCTGGGAGG 
CCCCGAAGCA GAGGGCCGGT GGGCAGGACT GAACCGGGGG AAGGCCCGCA GCAGGAGATC 
AAGCGTCGGG ACCAATTCCC GCTAACCAGG AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT 
GTGCCGCCCT ACACCGCGGA TCCCCCGGAG AGCAGGACAA AAGCCCCCTC CGGTCGCAAG 
CGGGAACCCC CTGCTCAAGC GGTGCGCTTT CTGCCCTGGG CCACTCCGGG AGTGGAGGAC 
TCTGTTCTGC CCCAAACCTT GGAGAAGAAT AGAGCGGGAC CCGAGGCTGA GAAGAGGCTT 
CGCAGAGGAC CTGAGGAGGA TGGCCCCTGG GGGCCCTGGG ACCGCAGAGG GACCCGCAGC 
CAAGGCAAAG GTCGCCGTGC TCGGCCTACT TCCCCCGAGC TCGAGTCCTC AGACGACTCC 
TATGTGTCCG CTGGGGAAGA GCCCCTGGAG GCACCCGTGT TTGAGATCCC TCTGCAGAAT 
ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_030243368.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099228.1 Mus musculus SPEG complex locus (Speg), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OMu84986
Clone ID Related Accession (Same CDS sequence)	XM_030243368.1
Accession Version	XM_030243368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
TACGACTCGG AGACTGCCGA GGATGACATC AGCGATGTGC CGGGGACCCA GCGCCTGGAG 
CTTCGGGATG ACAGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGTACC 
TCCCTGGACA CACCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG 
GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC 
CCAGGCAGCC CAAGCAGCAT TCCTCCGAGT GGATTGCACA GAGAGGAGCC TGACCTTCAG 
CCTCAGCCAG CCAGTGATGC CCTTCGTCCA CGCCCTGCCC TTCCACCTCC CTCCAAATCC 
GCTTTGCTTC CCCCACCATC TCCTCGGGTG GGAAAGAGGG CTCTGCCAGG ACCTAGTACC 
CAGCCCCCAG CTACTCCCAC TTCGCCCCAT CGGCGTGCTC AGGAGCCCTC GCTGCCTGAG 
GACATCACCA CCACGGAAGA GAAGCGAGGG AAAAAGCCCA AGTCATCCGG GCCGTCGCTG 
GCGGGCACAG TGGAGTCCCG GCCCCAGACG CCACTGAGCG AGGCTTCGGG TCGCCTGTCA 
GCATTGGGCC GCTCGCCCCG GCTGGTGCGC GCGGGGTCCC GCATCCTGGA CAAGCTACAG 
TTCTTCGAAG AGCGGCGACG CAGCCTGGAG CGCAGCGACT CGCCGCCAGC GCCCCTGCGG 
CCCTGGGTGC CCCTGCGCAA GGCTCGCTCG CTGGAGCAGC CGAAGTCCGA GGGCGGTGCG 
GCGTGGGGCA CACCCGAGGC CTCGCAGGAG GAGCTGCGGT CACCTCGGGG CAGTGTGGCA 
GAGCGGCGTC GCCTGTTCCA GCAAAAGGCG GCCTCGTTGG ATGAACGCAC GCGACAACGC 
AGTGCAACCT CGGACCTCGA ACTCCGCTTC GCCCAGGAGC TGGGTCGCAT CCGCCGATCT 
ACGTCGCGGG AGGAGCTGGT GCGTTCGCAC GAGTCCCTGC GTGCCACGCT GCAGCGCGCC 
CCATCCCCTC GGGAGCCCGG CGAGCCCCCA CTCTTCTCCC GGCCTTCCAC ACCCAAGACC 
TCACGGGCTG TGAGCCCGGC TGCCACCCAG CCGCCGCCTC CTAGTGGTGC GGGCAAATCT 
GGGGACGAGC CTGGGAGGCC CCGAAGCAGA GGGCCGGTGG GCAGGACTGA ACCGGGGGAA 
GGCCCGCAGC AGGAGATCAA GCGTCGGGAC CAATTCCCGC TAACCAGGAG CAGAGCCATC 
CAGGAGTGCA GGAGCCCTGT GCCGCCCTAC ACCGCGGATC CCCCGGAGAG CAGGACAAAA 
GCCCCCTCCG GTCGCAAGCG GGAACCCCCT GCTCAAGCGG TGCGCTTTCT GCCCTGGGCC 
ACTCCGGGAG TGGAGGACTC TGTTCTGCCC CAAACCTTGG AGAAGAATAG AGCGGGACCC 
GAGGCTGAGA AGAGGCTTCG CAGAGGACCT GAGGAGGATG GCCCCTGGGG GCCCTGGGAC 
CGCAGAGGGA CCCGCAGCCA AGGCAAAGGT CGCCGTGCTC GGCCTACTTC CCCCGAGCTC 
GAGTCCTCAG ACGACTCCTA TGTGTCCGCT GGGGAAGAGC CCCTGGAGGC ACCCGTGTTT 
GAGATCCCTC TGCAGAATAT GGTGGTGGCG CCAGGAGCTG ACGTGCTACT TAAGTGTATC 
ATCACCGCCA ACCCCCCACC CCAAGTGTCC TGGAAAAAGG ATGGGTCCAT GTTGCACAGC 
GAGGGTCGTC TTCTCATCCG GGCTGAAGGT GAACGGCACA CACTGCTGCT CAGAGAGGCC 
CAGGCTGCCG ATGCTGGGAG CTACACAGCC ACTGCCACCA ACGAACTGGG CCAAGCTACC 
TGTGCTTCTT CACTGGCTGT GAGACCTGGC GGCTCCACAT CCCCTTTCAG CAGCCCCATC 
ACCTCTGATG AGGAGTACCT GAGCCCCCCA GAGGAGTTCC CAGAGCCTGG GGAGACCTGG 
CCCCGAACCC CTACCATGAA GCTCAGTCCC AGCCAGGATC ATGATTCCTC CGACTCTTCT 
TCCAAGGCAC CCCCAACGTT CAAGGTCTCA CTCATGGACC AATCGGTGAG AGAAGGTCAA 
GATGTCATTA TGAGCATCCG TGTGCAGGGA GAGCCCAAGC CTGTGGTTTC CTGGCTGAGG 
AATCGACAGC CCGTGCGCCC AGACCAGCGG CGCTTTGCAG AGGAGGCCGA GGGTGGGCTC 
TGCCGCTTGA GGATCCTGGC TGCTGAACGG GGCGATGCTG GTTTCTACAC ATGCAAGGCG 
GTCAACGAAT ATGGCGCTCG GCAGTGCGAG GCCCGCCTGG AGGTCCGAGG CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099228.1
CDS	20..2776
Translation	MQKARGTRGEDAGTRAPPSPGVPPKRAKVGAGRGVLVTGDGAGAPVFLRPLKNAAVCAGSDVRLRVVVSGTPQPSLSWFRDGQLLPPPAPEPSCLWLRSCGAQDAGVYSCSAQNERGQASCEAVLTVLEVRVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPSSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	XM_030243368.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243368.1

ORF Insert Sequence:

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
TACGACTCGG AGACTGCCGA GGATGACATC AGCGATGTGC CGGGGACCCA GCGCCTGGAG 
CTTCGGGATG ACAGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGTACC 
TCCCTGGACA CACCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG 
GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC 
CCAGGCAGCC CAAGCAGCAT TCCTCCGAGT GGATTGCACA GAGAGGAGCC TGACCTTCAG 
CCTCAGCCAG CCAGTGATGC CCTTCGTCCA CGCCCTGCCC TTCCACCTCC CTCCAAATCC 
GCTTTGCTTC CCCCACCATC TCCTCGGGTG GGAAAGAGGG CTCTGCCAGG ACCTAGTACC 
CAGCCCCCAG CTACTCCCAC TTCGCCCCAT CGGCGTGCTC AGGAGCCCTC GCTGCCTGAG 
GACATCACCA CCACGGAAGA GAAGCGAGGG AAAAAGCCCA AGTCATCCGG GCCGTCGCTG 
GCGGGCACAG TGGAGTCCCG GCCCCAGACG CCACTGAGCG AGGCTTCGGG TCGCCTGTCA 
GCATTGGGCC GCTCGCCCCG GCTGGTGCGC GCGGGGTCCC GCATCCTGGA CAAGCTACAG 
TTCTTCGAAG AGCGGCGACG CAGCCTGGAG CGCAGCGACT CGCCGCCAGC GCCCCTGCGG 
CCCTGGGTGC CCCTGCGCAA GGCTCGCTCG CTGGAGCAGC CGAAGTCCGA GGGCGGTGCG 
GCGTGGGGCA CACCCGAGGC CTCGCAGGAG GAGCTGCGGT CACCTCGGGG CAGTGTGGCA 
GAGCGGCGTC GCCTGTTCCA GCAAAAGGCG GCCTCGTTGG ATGAACGCAC GCGACAACGC 
AGTGCAACCT CGGACCTCGA ACTCCGCTTC GCCCAGGAGC TGGGTCGCAT CCGCCGATCT 
ACGTCGCGGG AGGAGCTGGT GCGTTCGCAC GAGTCCCTGC GTGCCACGCT GCAGCGCGCC 
CCATCCCCTC GGGAGCCCGG CGAGCCCCCA CTCTTCTCCC GGCCTTCCAC ACCCAAGACC 
TCACGGGCTG TGAGCCCGGC TGCCACCCAG CCGCCGCCTC CTAGTGGTGC GGGCAAATCT 
GGGGACGAGC CTGGGAGGCC CCGAAGCAGA GGGCCGGTGG GCAGGACTGA ACCGGGGGAA 
GGCCCGCAGC AGGAGATCAA GCGTCGGGAC CAATTCCCGC TAACCAGGAG CAGAGCCATC 
CAGGAGTGCA GGAGCCCTGT GCCGCCCTAC ACCGCGGATC CCCCGGAGAG CAGGACAAAA 
GCCCCCTCCG GTCGCAAGCG GGAACCCCCT GCTCAAGCGG TGCGCTTTCT GCCCTGGGCC 
ACTCCGGGAG TGGAGGACTC TGTTCTGCCC CAAACCTTGG AGAAGAATAG AGCGGGACCC 
GAGGCTGAGA AGAGGCTTCG CAGAGGACCT GAGGAGGATG GCCCCTGGGG GCCCTGGGAC 
CGCAGAGGGA CCCGCAGCCA AGGCAAAGGT CGCCGTGCTC GGCCTACTTC CCCCGAGCTC 
GAGTCCTCAG ACGACTCCTA TGTGTCCGCT GGGGAAGAGC CCCTGGAGGC ACCCGTGTTT 
GAGATCCCTC TGCAGAATAT GGTGGTGGCG CCAGGAGCTG ACGTGCTACT TAAGTGTATC 
ATCACCGCCA ACCCCCCACC CCAAGTGTCC TGGAAAAAGG ATGGGTCCAT GTTGCACAGC 
GAGGGTCGTC TTCTCATCCG GGCTGAAGGT GAACGGCACA CACTGCTGCT CAGAGAGGCC 
CAGGCTGCCG ATGCTGGGAG CTACACAGCC ACTGCCACCA ACGAACTGGG CCAAGCTACC 
TGTGCTTCTT CACTGGCTGT GAGACCTGGC GGCTCCACAT CCCCTTTCAG CAGCCCCATC 
ACCTCTGATG AGGAGTACCT GAGCCCCCCA GAGGAGTTCC CAGAGCCTGG GGAGACCTGG 
CCCCGAACCC CTACCATGAA GCTCAGTCCC AGCCAGGATC ATGATTCCTC CGACTCTTCT 
TCCAAGGCAC CCCCAACGTT CAAGGTCTCA CTCATGGACC AATCGGTGAG AGAAGGTCAA 
GATGTCATTA TGAGCATCCG TGTGCAGGGA GAGCCCAAGC CTGTGGTTTC CTGGCTGAGG 
AATCGACAGC CCGTGCGCCC AGACCAGCGG CGCTTTGCAG AGGAGGCCGA GGGTGGGCTC 
TGCCGCTTGA GGATCCTGGC TGCTGAACGG GGCGATGCTG GTTTCTACAC ATGCAAGGCG 
GTCAACGAAT ATGGCGCTCG GCAGTGCGAG GCCCGCCTGG AGGTCCGAGG CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_030243390.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099250.1 Mus musculus SPEG complex locus (Speg), transcript variant X21, mRNA.

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CloneID	OMu84987
Clone ID Related Accession (Same CDS sequence)	XM_030243390.1
Accession Version	XM_030243390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACC AGTGTTGGTA CCTTCTGGGG AGGGGGTCTC CCTCTCCTCC CCATCTCACA 
GAACATCTCT GGTATCCCTG GACAGCAGGG ACCCTCATGC CTCCCTATAT TCTGCCTGCC 
TTCCATGCCC CATCCCACAA ACACTCTGAT AACTGTCTGT CCACATCTCT CTCCTGCTGC 
TCCTATGGAA GCGAAGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA 
GCACAGACAA CCGGGGCCGG GCCACGGCAC CTGGGGGTGG AGCCGCTGGT GCGGGCATCG 
CGAGCTAATC TGGTGGGCGC AAGCTGGGGG TCAGAGGATA GCCTTTCGGT GGCCAGTGAC 
CTCTATGGCA GCGCGTTCAG TCTGTACAGA GGACGGGCGC TCTCGATCCA CGTCAGCATT 
CCTCCGAGTG GATTGCACAG AGAGGAGCCT GACCTTCAGC CTCAGCCAGC CAGTGATGCC 
CTTCGTCCAC GCCCTGCCCT TCCACCTCCC TCCAAATCCG CTTTGCTTCC CCCACCATCT 
CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA CCTAGTACCC AGCCCCCAGC TACTCCCACT 
TCGCCCCATC GGCGTGCTCA GGAGCCCTCG CTGCCTGAGG ACATCACCAC CACGGAAGAG 
AAGCGAGGGA AAAAGCCCAA GTCATCCGGG CCGTCGCTGG CGGGCACAGT GGAGTCCCGG 
CCCCAGACGC CACTGAGCGA GGCTTCGGGT CGCCTGTCAG CATTGGGCCG CTCGCCCCGG 
CTGGTGCGCG CGGGGTCCCG CATCCTGGAC AAGCTACAGT TCTTCGAAGA GCGGCGACGC 
AGCCTGGAGC GCAGCGACTC GCCGCCAGCG CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG 
GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC 
TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG 
CAAAAGGCGG CCTCGTTGGA TGAACGCACG CGACAACGCA GTGCAACCTC GGACCTCGAA 
CTCCGCTTCG CCCAGGAGCT GGGTCGCATC CGCCGATCTA CGTCGCGGGA GGAGCTGGTG 
CGTTCGCACG AGTCCCTGCG TGCCACGCTG CAGCGCGCCC CATCCCCTCG GGAGCCCGGC 
GAGCCCCCAC TCTTCTCCCG GCCTTCCACA CCCAAGACCT CACGGGCTGT GAGCCCGGCT 
GCCACCCAGC CGCCGCCTCC TAGTGGTGCG GGCAAATCTG GGGACGAGCC TGGGAGGCCC 
CGAAGCAGAG GGCCGGTGGG CAGGACTGAA CCGGGGGAAG GCCCGCAGCA GGAGATCAAG 
CGTCGGGACC AATTCCCGCT AACCAGGAGC AGAGCCATCC AGGAGTGCAG GAGCCCTGTG 
CCGCCCTACA CCGCGGATCC CCCGGAGAGC AGGACAAAAG CCCCCTCCGG TCGCAAGCGG 
GAACCCCCTG CTCAAGCGGT GCGCTTTCTG CCCTGGGCCA CTCCGGGAGT GGAGGACTCT 
GTTCTGCCCC AAACCTTGGA GAAGAATAGA GCGGGACCCG AGGCTGAGAA GAGGCTTCGC 
AGAGGACCTG AGGAGGATGG CCCCTGGGGG CCCTGGGACC GCAGAGGGAC CCGCAGCCAA 
GGCAAAGGTC GCCGTGCTCG GCCTACTTCC CCCGAGCTCG AGTCCTCAGA CGACTCCTAT 
GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA CCCGTGTTTG AGATCCCTCT GCAGAATATG 
GTGGTGGCGC CAGGAGCTGA CGTGCTACTT AAGTGTATCA TCACCGCCAA CCCCCCACCC 
CAAGTGTCCT GGAAAAAGGA TGGGTCCATG TTGCACAGCG AGGGTCGTCT TCTCATCCGG 
GCTGAAGGTG AACGGCACAC ACTGCTGCTC AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC 
TACACAGCCA CTGCCACCAA CGAACTGGGC CAAGCTACCT GTGCTTCTTC ACTGGCTGTG 
AGACCTGGCG GCTCCACATC CCCTTTCAGC AGCCCCATCA CCTCTGATGA GGAGTACCTG 
AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG GAGACCTGGC CCCGAACCCC TACCATGAAG 
CTCAGTCCCA GCCAGGATCA TGATTCCTCC GACTCTTCTT CCAAGGCACC CCCAACGTTC 
AAGGTCTCAC TCATGGACCA ATCGGTGAGA GAAGGTCAAG ATGTCATTAT GAGCATCCGT 
GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC TGGCTGAGGA ATCGACAGCC CGTGCGCCCA 
GACCAGCGGC GCTTTGCAGA GGAGGCCGAG GGTGGGCTCT GCCGCTTGAG GATCCTGGCT 
GCTGAACGGG GCGATGCTGG TTTCTACACA TGCAAGGCGG TCAACGAATA TGGCGCTCGG 
CAGTGCGAGG CCCGCCTGGA GGTCCGAGGC GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099250.1
CDS	1992..4727
Translation	MAHQCWYLLGRGSPSPPHLTEHLWYPWTAGTLMPPYILPAFHAPSHKHSDNCLSTSLSCCSYGSEVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPRQAQTTGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	XM_030243390.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243390.1

ORF Insert Sequence:

ATGGCTCACC AGTGTTGGTA CCTTCTGGGG AGGGGGTCTC CCTCTCCTCC CCATCTCACA 
GAACATCTCT GGTATCCCTG GACAGCAGGG ACCCTCATGC CTCCCTATAT TCTGCCTGCC 
TTCCATGCCC CATCCCACAA ACACTCTGAT AACTGTCTGT CCACATCTCT CTCCTGCTGC 
TCCTATGGAA GCGAAGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA 
GCACAGACAA CCGGGGCCGG GCCACGGCAC CTGGGGGTGG AGCCGCTGGT GCGGGCATCG 
CGAGCTAATC TGGTGGGCGC AAGCTGGGGG TCAGAGGATA GCCTTTCGGT GGCCAGTGAC 
CTCTATGGCA GCGCGTTCAG TCTGTACAGA GGACGGGCGC TCTCGATCCA CGTCAGCATT 
CCTCCGAGTG GATTGCACAG AGAGGAGCCT GACCTTCAGC CTCAGCCAGC CAGTGATGCC 
CTTCGTCCAC GCCCTGCCCT TCCACCTCCC TCCAAATCCG CTTTGCTTCC CCCACCATCT 
CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA CCTAGTACCC AGCCCCCAGC TACTCCCACT 
TCGCCCCATC GGCGTGCTCA GGAGCCCTCG CTGCCTGAGG ACATCACCAC CACGGAAGAG 
AAGCGAGGGA AAAAGCCCAA GTCATCCGGG CCGTCGCTGG CGGGCACAGT GGAGTCCCGG 
CCCCAGACGC CACTGAGCGA GGCTTCGGGT CGCCTGTCAG CATTGGGCCG CTCGCCCCGG 
CTGGTGCGCG CGGGGTCCCG CATCCTGGAC AAGCTACAGT TCTTCGAAGA GCGGCGACGC 
AGCCTGGAGC GCAGCGACTC GCCGCCAGCG CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG 
GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC 
TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG 
CAAAAGGCGG CCTCGTTGGA TGAACGCACG CGACAACGCA GTGCAACCTC GGACCTCGAA 
CTCCGCTTCG CCCAGGAGCT GGGTCGCATC CGCCGATCTA CGTCGCGGGA GGAGCTGGTG 
CGTTCGCACG AGTCCCTGCG TGCCACGCTG CAGCGCGCCC CATCCCCTCG GGAGCCCGGC 
GAGCCCCCAC TCTTCTCCCG GCCTTCCACA CCCAAGACCT CACGGGCTGT GAGCCCGGCT 
GCCACCCAGC CGCCGCCTCC TAGTGGTGCG GGCAAATCTG GGGACGAGCC TGGGAGGCCC 
CGAAGCAGAG GGCCGGTGGG CAGGACTGAA CCGGGGGAAG GCCCGCAGCA GGAGATCAAG 
CGTCGGGACC AATTCCCGCT AACCAGGAGC AGAGCCATCC AGGAGTGCAG GAGCCCTGTG 
CCGCCCTACA CCGCGGATCC CCCGGAGAGC AGGACAAAAG CCCCCTCCGG TCGCAAGCGG 
GAACCCCCTG CTCAAGCGGT GCGCTTTCTG CCCTGGGCCA CTCCGGGAGT GGAGGACTCT 
GTTCTGCCCC AAACCTTGGA GAAGAATAGA GCGGGACCCG AGGCTGAGAA GAGGCTTCGC 
AGAGGACCTG AGGAGGATGG CCCCTGGGGG CCCTGGGACC GCAGAGGGAC CCGCAGCCAA 
GGCAAAGGTC GCCGTGCTCG GCCTACTTCC CCCGAGCTCG AGTCCTCAGA CGACTCCTAT 
GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA CCCGTGTTTG AGATCCCTCT GCAGAATATG 
GTGGTGGCGC CAGGAGCTGA CGTGCTACTT AAGTGTATCA TCACCGCCAA CCCCCCACCC 
CAAGTGTCCT GGAAAAAGGA TGGGTCCATG TTGCACAGCG AGGGTCGTCT TCTCATCCGG 
GCTGAAGGTG AACGGCACAC ACTGCTGCTC AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC 
TACACAGCCA CTGCCACCAA CGAACTGGGC CAAGCTACCT GTGCTTCTTC ACTGGCTGTG 
AGACCTGGCG GCTCCACATC CCCTTTCAGC AGCCCCATCA CCTCTGATGA GGAGTACCTG 
AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG GAGACCTGGC CCCGAACCCC TACCATGAAG 
CTCAGTCCCA GCCAGGATCA TGATTCCTCC GACTCTTCTT CCAAGGCACC CCCAACGTTC 
AAGGTCTCAC TCATGGACCA ATCGGTGAGA GAAGGTCAAG ATGTCATTAT GAGCATCCGT 
GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC TGGCTGAGGA ATCGACAGCC CGTGCGCCCA 
GACCAGCGGC GCTTTGCAGA GGAGGCCGAG GGTGGGCTCT GCCGCTTGAG GATCCTGGCT 
GCTGAACGGG GCGATGCTGG TTTCTACACA TGCAAGGCGG TCAACGAATA TGGCGCTCGG 
CAGTGCGAGG CCCGCCTGGA GGTCCGAGGC GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_030243402.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099262.1 Mus musculus SPEG complex locus (Speg), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OMu84988
Clone ID Related Accession (Same CDS sequence)	XM_030243402.1
Accession Version	XM_030243402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA 
GCACAGACAA CCGGGGCCGG GCCACGGCAC CTGGGGGTGG AGCCGCTGGT GCGGGCATCG 
CGAGCTAATC TGGTGGGCGC AAGCTGGGGG TCAGAGGATA GCCTTTCGGT GGCCAGTGAC 
CTCTATGGCA GCGCGTTCAG TCTGTACAGA GGACGGGCGC TCTCGATCCA CGTCAGCATT 
CCTCCGAGTG GATTGCACAG AGAGGAGCCT GACCTTCAGC CTCAGCCAGC CAGTGATGCC 
CTTCGTCCAC GCCCTGCCCT TCCACCTCCC TCCAAATCCG CTTTGCTTCC CCCACCATCT 
CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA CCTAGTACCC AGCCCCCAGC TACTCCCACT 
TCGCCCCATC GGCGTGCTCA GGAGCCCTCG CTGCCTGAGG ACATCACCAC CACGGAAGAG 
AAGCGAGGGA AAAAGCCCAA GTCATCCGGG CCGTCGCTGG CGGGCACAGT GGAGTCCCGG 
CCCCAGACGC CACTGAGCGA GGCTTCGGGT CGCCTGTCAG CATTGGGCCG CTCGCCCCGG 
CTGGTGCGCG CGGGGTCCCG CATCCTGGAC AAGCTACAGT TCTTCGAAGA GCGGCGACGC 
AGCCTGGAGC GCAGCGACTC GCCGCCAGCG CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG 
GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC 
TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG 
CAAAAGGCGG CCTCGTTGGA TGAACGCACG CGACAACGCA GTGCAACCTC GGACCTCGAA 
CTCCGCTTCG CCCAGGAGCT GGGTCGCATC CGCCGATCTA CGTCGCGGGA GGAGCTGGTG 
CGTTCGCACG AGTCCCTGCG TGCCACGCTG CAGCGCGCCC CATCCCCTCG GGAGCCCGGC 
GAGCCCCCAC TCTTCTCCCG GCCTTCCACA CCCAAGACCT CACGGGCTGT GAGCCCGGCT 
GCCACCCAGC CGCCGCCTCC TAGTGGTGCG GGCAAATCTG GGGACGAGCC TGGGAGGCCC 
CGAAGCAGAG GGCCGGTGGG CAGGACTGAA CCGGGGGAAG GCCCGCAGCA GGAGATCAAG 
CGTCGGGACC AATTCCCGCT AACCAGGAGC AGAGCCATCC AGGAGTGCAG GAGCCCTGTG 
CCGCCCTACA CCGCGGATCC CCCGGAGAGC AGGACAAAAG CCCCCTCCGG TCGCAAGCGG 
GAACCCCCTG CTCAAGCGGT GCGCTTTCTG CCCTGGGCCA CTCCGGGAGT GGAGGACTCT 
GTTCTGCCCC AAACCTTGGA GAAGAATAGA GCGGGACCCG AGGCTGAGAA GAGGCTTCGC 
AGAGGACCTG AGGAGGATGG CCCCTGGGGG CCCTGGGACC GCAGAGGGAC CCGCAGCCAA 
GGCAAAGGTC GCCGTGCTCG GCCTACTTCC CCCGAGCTCG AGTCCTCAGA CGACTCCTAT 
GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA CCCGTGTTTG AGATCCCTCT GCAGAATATG 
GTGGTGGCGC CAGGAGCTGA CGTGCTACTT AAGTGTATCA TCACCGCCAA CCCCCCACCC 
CAAGTGTCCT GGAAAAAGGA TGGGTCCATG TTGCACAGCG AGGGTCGTCT TCTCATCCGG 
GCTGAAGGTG AACGGCACAC ACTGCTGCTC AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC 
TACACAGCCA CTGCCACCAA CGAACTGGGC CAAGCTACCT GTGCTTCTTC ACTGGCTGTG 
AGACCTGGCG GCTCCACATC CCCTTTCAGC AGCCCCATCA CCTCTGATGA GGAGTACCTG 
AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG GAGACCTGGC CCCGAACCCC TACCATGAAG 
CTCAGTCCCA GCCAGGATCA TGATTCCTCC GACTCTTCTT CCAAGGCACC CCCAACGTTC 
AAGGTCTCAC TCATGGACCA ATCGGTGAGA GAAGGTCAAG ATGTCATTAT GAGCATCCGT 
GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC TGGCTGAGGA ATCGACAGCC CGTGCGCCCA 
GACCAGCGGC GCTTTGCAGA GGAGGCCGAG GGTGGGCTCT GCCGCTTGAG GATCCTGGCT 
GCTGAACGGG GCGATGCTGG TTTCTACACA TGCAAGGCGG TCAACGAATA TGGCGCTCGG 
CAGTGCGAGG CCCGCCTGGA GGTCCGAGGC GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099262.1
CDS	1339..3954
Translation	MKKLWVKKRFQKTGHSRRAFGRLTHVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPRQAQTTGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	XM_030243402.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243402.1

ORF Insert Sequence:

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA 
GCACAGACAA CCGGGGCCGG GCCACGGCAC CTGGGGGTGG AGCCGCTGGT GCGGGCATCG 
CGAGCTAATC TGGTGGGCGC AAGCTGGGGG TCAGAGGATA GCCTTTCGGT GGCCAGTGAC 
CTCTATGGCA GCGCGTTCAG TCTGTACAGA GGACGGGCGC TCTCGATCCA CGTCAGCATT 
CCTCCGAGTG GATTGCACAG AGAGGAGCCT GACCTTCAGC CTCAGCCAGC CAGTGATGCC 
CTTCGTCCAC GCCCTGCCCT TCCACCTCCC TCCAAATCCG CTTTGCTTCC CCCACCATCT 
CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA CCTAGTACCC AGCCCCCAGC TACTCCCACT 
TCGCCCCATC GGCGTGCTCA GGAGCCCTCG CTGCCTGAGG ACATCACCAC CACGGAAGAG 
AAGCGAGGGA AAAAGCCCAA GTCATCCGGG CCGTCGCTGG CGGGCACAGT GGAGTCCCGG 
CCCCAGACGC CACTGAGCGA GGCTTCGGGT CGCCTGTCAG CATTGGGCCG CTCGCCCCGG 
CTGGTGCGCG CGGGGTCCCG CATCCTGGAC AAGCTACAGT TCTTCGAAGA GCGGCGACGC 
AGCCTGGAGC GCAGCGACTC GCCGCCAGCG CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG 
GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC 
TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG 
CAAAAGGCGG CCTCGTTGGA TGAACGCACG CGACAACGCA GTGCAACCTC GGACCTCGAA 
CTCCGCTTCG CCCAGGAGCT GGGTCGCATC CGCCGATCTA CGTCGCGGGA GGAGCTGGTG 
CGTTCGCACG AGTCCCTGCG TGCCACGCTG CAGCGCGCCC CATCCCCTCG GGAGCCCGGC 
GAGCCCCCAC TCTTCTCCCG GCCTTCCACA CCCAAGACCT CACGGGCTGT GAGCCCGGCT 
GCCACCCAGC CGCCGCCTCC TAGTGGTGCG GGCAAATCTG GGGACGAGCC TGGGAGGCCC 
CGAAGCAGAG GGCCGGTGGG CAGGACTGAA CCGGGGGAAG GCCCGCAGCA GGAGATCAAG 
CGTCGGGACC AATTCCCGCT AACCAGGAGC AGAGCCATCC AGGAGTGCAG GAGCCCTGTG 
CCGCCCTACA CCGCGGATCC CCCGGAGAGC AGGACAAAAG CCCCCTCCGG TCGCAAGCGG 
GAACCCCCTG CTCAAGCGGT GCGCTTTCTG CCCTGGGCCA CTCCGGGAGT GGAGGACTCT 
GTTCTGCCCC AAACCTTGGA GAAGAATAGA GCGGGACCCG AGGCTGAGAA GAGGCTTCGC 
AGAGGACCTG AGGAGGATGG CCCCTGGGGG CCCTGGGACC GCAGAGGGAC CCGCAGCCAA 
GGCAAAGGTC GCCGTGCTCG GCCTACTTCC CCCGAGCTCG AGTCCTCAGA CGACTCCTAT 
GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA CCCGTGTTTG AGATCCCTCT GCAGAATATG 
GTGGTGGCGC CAGGAGCTGA CGTGCTACTT AAGTGTATCA TCACCGCCAA CCCCCCACCC 
CAAGTGTCCT GGAAAAAGGA TGGGTCCATG TTGCACAGCG AGGGTCGTCT TCTCATCCGG 
GCTGAAGGTG AACGGCACAC ACTGCTGCTC AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC 
TACACAGCCA CTGCCACCAA CGAACTGGGC CAAGCTACCT GTGCTTCTTC ACTGGCTGTG 
AGACCTGGCG GCTCCACATC CCCTTTCAGC AGCCCCATCA CCTCTGATGA GGAGTACCTG 
AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG GAGACCTGGC CCCGAACCCC TACCATGAAG 
CTCAGTCCCA GCCAGGATCA TGATTCCTCC GACTCTTCTT CCAAGGCACC CCCAACGTTC 
AAGGTCTCAC TCATGGACCA ATCGGTGAGA GAAGGTCAAG ATGTCATTAT GAGCATCCGT 
GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC TGGCTGAGGA ATCGACAGCC CGTGCGCCCA 
GACCAGCGGC GCTTTGCAGA GGAGGCCGAG GGTGGGCTCT GCCGCTTGAG GATCCTGGCT 
GCTGAACGGG GCGATGCTGG TTTCTACACA TGCAAGGCGG TCAACGAATA TGGCGCTCGG 
CAGTGCGAGG CCCGCCTGGA GGTCCGAGGC GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_030243447.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099307.1 Mus musculus SPEG complex locus (Speg), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OMu84989
Clone ID Related Accession (Same CDS sequence)	XM_030243447.1
Accession Version	XM_030243447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGCAGC 
ATTCCTCCGA GTGGATTGCA CAGAGAGGAG CCTGACCTTC AGCCTCAGCC AGCCAGTGAT 
GCCCTTCGTC CACGCCCTGC CCTTCCACCT CCCTCCAAAT CCGCTTTGCT TCCCCCACCA 
TCTCCTCGGG TGGGAAAGAG GGCTCTGCCA GGACCTAGTA CCCAGCCCCC AGCTACTCCC 
ACTTCGCCCC ATCGGCGTGC TCAGGAGCCC TCGCTGCCTG AGGACATCAC CACCACGGAA 
GAGAAGCGAG GGAAAAAGCC CAAGTCATCC GGGCCGTCGC TGGCGGGCAC AGTGGAGTCC 
CGGCCCCAGA CGCCACTGAG CGAGGCTTCG GGTCGCCTGT CAGCATTGGG CCGCTCGCCC 
CGGCTGGTGC GCGCGGGGTC CCGCATCCTG GACAAGCTAC AGTTCTTCGA AGAGCGGCGA 
CGCAGCCTGG AGCGCAGCGA CTCGCCGCCA GCGCCCCTGC GGCCCTGGGT GCCCCTGCGC 
AAGGCTCGCT CGCTGGAGCA GCCGAAGTCC GAGGGCGGTG CGGCGTGGGG CACACCCGAG 
GCCTCGCAGG AGGAGCTGCG GTCACCTCGG GGCAGTGTGG CAGAGCGGCG TCGCCTGTTC 
CAGCAAAAGG CGGCCTCGTT GGATGAACGC ACGCGACAAC GCAGTGCAAC CTCGGACCTC 
GAACTCCGCT TCGCCCAGGA GCTGGGTCGC ATCCGCCGAT CTACGTCGCG GGAGGAGCTG 
GTGCGTTCGC ACGAGTCCCT GCGTGCCACG CTGCAGCGCG CCCCATCCCC TCGGGAGCCC 
GGCGAGCCCC CACTCTTCTC CCGGCCTTCC ACACCCAAGA CCTCACGGGC TGTGAGCCCG 
GCTGCCACCC AGCCGCCGCC TCCTAGTGGT GCGGGCAAAT CTGGGGACGA GCCTGGGAGG 
CCCCGAAGCA GAGGGCCGGT GGGCAGGACT GAACCGGGGG AAGGCCCGCA GCAGGAGATC 
AAGCGTCGGG ACCAATTCCC GCTAACCAGG AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT 
GTGCCGCCCT ACACCGCGGA TCCCCCGGAG AGCAGGACAA AAGCCCCCTC CGGTCGCAAG 
CGGGAACCCC CTGCTCAAGC GGTGCGCTTT CTGCCCTGGG CCACTCCGGG AGTGGAGGAC 
TCTGTTCTGC CCCAAACCTT GGAGAAGAAT AGAGCGGGAC CCGAGGCTGA GAAGAGGCTT 
CGCAGAGGAC CTGAGGAGGA TGGCCCCTGG GGGCCCTGGG ACCGCAGAGG GACCCGCAGC 
CAAGGCAAAG GTCGCCGTGC TCGGCCTACT TCCCCCGAGC TCGAGTCCTC AGACGACTCC 
TATGTGTCCG CTGGGGAAGA GCCCCTGGAG GCACCCGTGT TTGAGATCCC TCTGCAGAAT 
ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099307.1
CDS	1337..3775
Translation	MKKLWVKKRFQKTGHSRRAFGRLTHVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPSSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRGE

Target ORF information:

RefSeq Version	XM_030243447.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243447.1

ORF Insert Sequence:

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGCAGC 
ATTCCTCCGA GTGGATTGCA CAGAGAGGAG CCTGACCTTC AGCCTCAGCC AGCCAGTGAT 
GCCCTTCGTC CACGCCCTGC CCTTCCACCT CCCTCCAAAT CCGCTTTGCT TCCCCCACCA 
TCTCCTCGGG TGGGAAAGAG GGCTCTGCCA GGACCTAGTA CCCAGCCCCC AGCTACTCCC 
ACTTCGCCCC ATCGGCGTGC TCAGGAGCCC TCGCTGCCTG AGGACATCAC CACCACGGAA 
GAGAAGCGAG GGAAAAAGCC CAAGTCATCC GGGCCGTCGC TGGCGGGCAC AGTGGAGTCC 
CGGCCCCAGA CGCCACTGAG CGAGGCTTCG GGTCGCCTGT CAGCATTGGG CCGCTCGCCC 
CGGCTGGTGC GCGCGGGGTC CCGCATCCTG GACAAGCTAC AGTTCTTCGA AGAGCGGCGA 
CGCAGCCTGG AGCGCAGCGA CTCGCCGCCA GCGCCCCTGC GGCCCTGGGT GCCCCTGCGC 
AAGGCTCGCT CGCTGGAGCA GCCGAAGTCC GAGGGCGGTG CGGCGTGGGG CACACCCGAG 
GCCTCGCAGG AGGAGCTGCG GTCACCTCGG GGCAGTGTGG CAGAGCGGCG TCGCCTGTTC 
CAGCAAAAGG CGGCCTCGTT GGATGAACGC ACGCGACAAC GCAGTGCAAC CTCGGACCTC 
GAACTCCGCT TCGCCCAGGA GCTGGGTCGC ATCCGCCGAT CTACGTCGCG GGAGGAGCTG 
GTGCGTTCGC ACGAGTCCCT GCGTGCCACG CTGCAGCGCG CCCCATCCCC TCGGGAGCCC 
GGCGAGCCCC CACTCTTCTC CCGGCCTTCC ACACCCAAGA CCTCACGGGC TGTGAGCCCG 
GCTGCCACCC AGCCGCCGCC TCCTAGTGGT GCGGGCAAAT CTGGGGACGA GCCTGGGAGG 
CCCCGAAGCA GAGGGCCGGT GGGCAGGACT GAACCGGGGG AAGGCCCGCA GCAGGAGATC 
AAGCGTCGGG ACCAATTCCC GCTAACCAGG AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT 
GTGCCGCCCT ACACCGCGGA TCCCCCGGAG AGCAGGACAA AAGCCCCCTC CGGTCGCAAG 
CGGGAACCCC CTGCTCAAGC GGTGCGCTTT CTGCCCTGGG CCACTCCGGG AGTGGAGGAC 
TCTGTTCTGC CCCAAACCTT GGAGAAGAAT AGAGCGGGAC CCGAGGCTGA GAAGAGGCTT 
CGCAGAGGAC CTGAGGAGGA TGGCCCCTGG GGGCCCTGGG ACCGCAGAGG GACCCGCAGC 
CAAGGCAAAG GTCGCCGTGC TCGGCCTACT TCCCCCGAGC TCGAGTCCTC AGACGACTCC 
TATGTGTCCG CTGGGGAAGA GCCCCTGGAG GCACCCGTGT TTGAGATCCC TCTGCAGAAT 
ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_030243348.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099208.1 Mus musculus SPEG complex locus (Speg), transcript variant X18, mRNA.

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CloneID	OMu84985
Clone ID Related Accession (Same CDS sequence)	XM_030243348.1
Accession Version	XM_030243348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTCAAG TGCTAGAGGG GATATGCTAT CTGCATCAGA GCCATGTGCT GCACCTGGAT 
GTCAAGCCGG AGAACCTGCT TGTGTGGGAT GGTGCAGGGG GTGAAGAGCA GGTGCGCATC 
TGTGACTTCG GGAACGCCCA GGAACTCACT CCGGGAGAGC CCCAGTACTG TCAGTATGGC 
ACGCCAGAGT TTGTAGCACC TGAGATTGTC AACCAGAGCC CTGTGTCTGG AGTCACTGAC 
ATCTGGCCCG TGGGCGTCGT TGCCTTTCTC TGTTTGACGG GCATCTCCCC ATTTGTTGGG 
GAGAATGACC GGACAACACT CATGAATATC CGAAACTACA ACGTGGCATT TGAGGAGACT 
ACGTTCCTGA GTCTGAGCAG GGAGGCCCGG GGCTTCCTCA TCAAAGTGCT GGTGCAGGAT 
CGACTGAGAC CTACTGCAGA AGAGACATTG GAACATCCTT GGTTCAAAAC AGAAGCAAAG 
GGTGCAGAGG TGAGCACAGA TCACCTGAAG CTCTTCCTGT CACGGAGGAG GTGGCAGCGC 
TCTCAAATCA GCTACAAGTG CCACCTGGTG CTGCGCCCCA TTCCGGAACT GCTACGGGCT 
CCCCCGGAGC GGGTGTGGGT GGCCATGCCT AGAAGACAAC CCCCGAGTGG GGGACTCTCA 
TCCTCTTCAG ACTCAGAAGA GGAAGAGTTG GAGGAACTTC CCTCAGTACC CCGCCCCCTG 
CAGCCTGAGT TCTCAGGTTC TCGGGTATCC CTCACCGACA TCCCCACCGA AGATGAGGCT 
CTGGGGACCC CGGAAGCTGG GGCTGCCACC CCCATGGACT GGCAAGAGCA GGAAAGGACT 
CCCTCTAAGG ACCAGGAGGC CCCCAGCCCT GAGGCACTCC CCTCTCCAGG CCAGGAGTCC 
CCTGATGGGC CTAGCCCCAG GCGGCCAGAG CTCCGCAGGG GTAGCTCTGC TGAGAGTGCC 
CTGCCCCGAG TCGGGTCGCG GGAACCGGGC CGGAGCTTGC ACAAGGCAGC ATCCGTGGAG 
CTGCCGCAGC GCAGGAGCCC CAGCCCGGGT GCCACCCGCC TGACTCGGGG AGGCCTGGGT 
GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC CTGCGTCAGC GGCTGCTGAG GGGAGGCCCC 
GAGGATGGCA AGGTCAGCGG CCTCAGGGGA CCCCTGCTGG AGAGCCTGGG GGGCCGTGCA 
CGGGATCCCA GGATGGCAAG GGCTGCCTCC AGCGAGGCTG CCCCCCACCA CCAGCCCCCT 
CCGGAGTCCC GGGGTCTGCA AAAGAGCAGC AGCTTCTCTC AGGGCGAGGC AGAGCCCCGG 
GGTCGGCACC GCCGCGCGGG GGCACCCCTC GAAATCCCCG TGGCCAGACT TGGGGCTCGC 
AGGCTGCAGG AGTCTCCCTC CTTATCTGCC CTTAGCGAGA CCCAGCCACC GAGCCCTGCG 
AGGCCCAGCG TCCCCAAACT CAGTATCACC AAGTCCCCGG AACCTTCCGC TGTCACATCC 
AGAGACTCTC CCCAGCCCCC GGAACCTCAG CCTGTCCCAG AAAAGGTCCC TGAGCCCAAA 
CCAGAACCAG TCCGAGCTGC AAAACCAGCA CAACCCCCTT TGGCTCTGCA AATGCCAACG 
CAGCCTCTCA CCCCCTATGC CCAGATCATG CAGTCGCTCC AGCTGTCCAG TCCCACCCTG 
TCCCCACAGG ACCCCGCTGT GCCTCCATCA GAGCCCAAGC CCCACGCGGC TGTGTTTGCC 
AGGGTGGCTT CCCCACCACC CGGAGTCTCT GAGAAGCGCG TGCCTTCGGC CAGAACTCCC 
CCAGTGCTAG CCGAGAAAGC CCGAGTCCCC ACGGTGCCCC CCAGGCCAGG CAGCAGCCTC 
AGCGGCAGCA TCGAAAACCT TGAATCAGAG GCTGTGTTCG AGGCTAAGTT CAAACGCAGC 
CGCGAATCGC CACTGTCGCG GGGCCTGAGG CTACTGAGCC GCTCGCGCTC GGAGGAGCGC 
GGTCCCTTCC GTGGGGCGGA GGACGACGGC ATATACCGGC CCAGTCCGGC AGGAACGCCG 
CTAGAGCTGG TGCGACGGCC CGAGCGCTCG CGCTCCGTGC AGGACCTCAG GGTAGCCGGA 
GAGCCGGGCC TTGTGCGTCG CCTTTCGCTG TCCCTGTCGC AGAAGCTGCG GCGGACACCG 
CCGGGACAGC GACACCCTGC CTGGGAGTCC CGCAGCGGAG ACGGGGAGAG CTCCGAGGGC 
GGCAGCTCAG CTCGGGCATC CCCAGTGCTG GCGGTACGCA GGAGACTCAG CTCCACGCTG 
GAGCGTCTGT CCAGCCGTTT GCAGCGCAGC GGCAGCAGCG AGGACTCCGG GGGCGCCTCG 
GGTCGCAGCA CGCCGCTGTT TGGACGGCTG CGCAGGGCTA CTTCGGAAGG GGAGAGCCTG 
CGACGCCTCG GTGTTCCGCA CAATCAGCTG GGCTCTCAGA CTGGTGCCAC TACACCTTCC 
GCTGAGTCTC TGGGCTCAGA GGCCAGTGGC ACATCGGGTT CTTCAGCTCC TGGAGAAAGC 
CGAAGCCGGC ACCGGTGGGG CCTTTCCAGG CTGCGCAAGG ACAAGGGATT ATCTCAGCCA 
AACCTCTCCT CCAGTGTCCA AGAGGACTTG GGTCACCAGT ATGTGCCCAG TGAGTCAGAT 
TTCCCCCCAG TCTTCCACAT CAAACTCAAG GACCAGGTGC TGTTGGAGGG GGAAGCAGCC 
ACCCTGCTCT GCCTACCCGC AGCCTGCCCA GCACCCCGCA TCTCCTGGAT GAAAGATAAG 
CAGTCCCTGC GTTCAGAGCC CTCAGTGGTC ATTGTGTCCT GCAAAGATGG ACGGCAGCTG 
CTGAGCATAC CCCGGGCCGG CAAGCGGCAT GCTGGGCTCT ATGAGTGCTC AGCCACCAAT 
GTCCTGGGCA GCATTACCAG CTCCTGTACT GTGGCCGTGG CCCGCATTCC AGGGAAGCTA 
GCTCCTCCAG AGGTGCCCCA GACCTACCAT GACACAGCAC TAGTAGTGTG GAAGCCAGGA 
GACGGTCGGG CACCTTGCAC ATACACTCTG GAGCGGCGGG TGGATGGGGA ATCTGTCTGG 
CACCCTGTGA GCTCAGGCAT CCCCGACTGT TATTACAATG TGACTCAGCT GCCTGTCGGC 
GTGACCGTGC GGTTCCGTGT GGCCTGTTCC AATCGTGCTG GGCAGGGGCC CTTCAGCAAC 
CCTTCTGAGA AGGTCTTCAT CCGAGGCACT CCAGATTCTC CTGCTCAGCC AGCTGCTGCT 
CCCCGAGATG CCCCTGTTAC CTCAGGGCCA ACCAGGGCCC CACCGCCTGA CTCTCCTACC 
TCACTGGCCC CCACCCCCGC TCTTGCGCCC CCAGCCTCCC AGGCATCCAC TCTCAGCCCC 
TCAACTTCCT CCATGTCTGC CAACCAGGCC TTGTCCTCAC TCAAGGCTGT GGGTCCACCA 
CCCGCAACCC CTCCACGAAA GCACAGGGGC CTTCTGGCCA CCCAGCAAGC AGAGCCATCC 
CCACCCAGTA TCGTGGTCAC CCCAAGTGAA CCCAGGTCTT TTGTCCCTGA CACTGGAACC 
CTGACCCCAA CCTCTAGTCC TCAAGGGGTT AAACCAGCAC CTTCCTCAAC TTCCTTGTAT 
ATGGTGACTT CCTTCGTGTC TGCACCTCCA GCCCCCCAGG CCCCAGCCCC TGAGCCCCCT 
CCTGAGCCCA CCAAGGTGAC TGTGCGGAGC CTCAGCCCAG CCAAGGAGGT GGTCAGCTCC 
CCAACTCCTG AGAGCACCAC TCTTCGGCAG GGTCCCCCTC AGAAACCCTA CACCTTCCTG 
GAGGAGAAGG CCAGGGGCCG CTTTGGCGTT GTGCGGTCAT GCCGGGAGAA TGCTACGGGC 
CGAACGTTTG TCGCCAAGAT CGTGCCGTAC GCCGCGGAGG GCAAGCGACG CGTGCTGCAG 
GAATACGAGG TGCTTCGGAC GCTGCACCAC GAGCGGCTCA TGTCCCTGCA CGAGGCCTAC 
ATCACCCCTC GGTACCTCGT GCTTATCGCT GAGAGCTGTG GCAACCGGGA ACTCCTCTGT 
GGGCTCAGCG ACAGATTCCG GTATTCAGAA GATGACGTGG CCACCTATGT GGTGCAGCTG 
CTACAAGGCC TGGACTACCT CCACGGCCAC CACGTGCTAC ACCTGGACAT CAAGCCGGAC 
AATCTACTGT TGGCCGCTGA CAATGCCCTT AAAATTGTGG ACTTCGGCAG TGCTCAGCCC 
TATAACCCTC AGGCCCTGAA GCCACTTGGC CACCGCACAG GCACTCTGGA GTTCATGGCT 
CCTGAGATGG TGAAGGGAGA CCCCATTGGC TCTGCCACGG ACATCTGGGG AGCGGGTGTG 
CTCACCTACA TCATGCTTAG TGGGTACTCC CCATTCTATG AGCCAGACCC CCAGGAAACA 
GAGGCTCGGA TTGTTGGGGG TCGCTTTGAT GCCTTCCAGT TGTATCCTAA CACATCCCAG 
AGTGCCACCC TCTTCTTGAG AAAGGTCCTC TCAGTACATC CCTGGAGCCG GCCCTCTCTG 
CAGGACTGCT TGGCCCACCC ATGGCTGCAA GATGCCTACC TGATGAAGCT GCGCCGCCAG 
ACACTCACCT TCACCACCAA CCGGCTCAAG GAATTCCTGG GCGAGCAGCG GCGACGTCGG 
GCTGAGGCTG CTACCCGTCA CAAGGTGCTG CTCCGCTCCT ACCCTGGCAG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099208.1
CDS	78..4754
Translation	MRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_030243348.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243348.1

ORF Insert Sequence:

ATGCGTCAAG TGCTAGAGGG GATATGCTAT CTGCATCAGA GCCATGTGCT GCACCTGGAT 
GTCAAGCCGG AGAACCTGCT TGTGTGGGAT GGTGCAGGGG GTGAAGAGCA GGTGCGCATC 
TGTGACTTCG GGAACGCCCA GGAACTCACT CCGGGAGAGC CCCAGTACTG TCAGTATGGC 
ACGCCAGAGT TTGTAGCACC TGAGATTGTC AACCAGAGCC CTGTGTCTGG AGTCACTGAC 
ATCTGGCCCG TGGGCGTCGT TGCCTTTCTC TGTTTGACGG GCATCTCCCC ATTTGTTGGG 
GAGAATGACC GGACAACACT CATGAATATC CGAAACTACA ACGTGGCATT TGAGGAGACT 
ACGTTCCTGA GTCTGAGCAG GGAGGCCCGG GGCTTCCTCA TCAAAGTGCT GGTGCAGGAT 
CGACTGAGAC CTACTGCAGA AGAGACATTG GAACATCCTT GGTTCAAAAC AGAAGCAAAG 
GGTGCAGAGG TGAGCACAGA TCACCTGAAG CTCTTCCTGT CACGGAGGAG GTGGCAGCGC 
TCTCAAATCA GCTACAAGTG CCACCTGGTG CTGCGCCCCA TTCCGGAACT GCTACGGGCT 
CCCCCGGAGC GGGTGTGGGT GGCCATGCCT AGAAGACAAC CCCCGAGTGG GGGACTCTCA 
TCCTCTTCAG ACTCAGAAGA GGAAGAGTTG GAGGAACTTC CCTCAGTACC CCGCCCCCTG 
CAGCCTGAGT TCTCAGGTTC TCGGGTATCC CTCACCGACA TCCCCACCGA AGATGAGGCT 
CTGGGGACCC CGGAAGCTGG GGCTGCCACC CCCATGGACT GGCAAGAGCA GGAAAGGACT 
CCCTCTAAGG ACCAGGAGGC CCCCAGCCCT GAGGCACTCC CCTCTCCAGG CCAGGAGTCC 
CCTGATGGGC CTAGCCCCAG GCGGCCAGAG CTCCGCAGGG GTAGCTCTGC TGAGAGTGCC 
CTGCCCCGAG TCGGGTCGCG GGAACCGGGC CGGAGCTTGC ACAAGGCAGC ATCCGTGGAG 
CTGCCGCAGC GCAGGAGCCC CAGCCCGGGT GCCACCCGCC TGACTCGGGG AGGCCTGGGT 
GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC CTGCGTCAGC GGCTGCTGAG GGGAGGCCCC 
GAGGATGGCA AGGTCAGCGG CCTCAGGGGA CCCCTGCTGG AGAGCCTGGG GGGCCGTGCA 
CGGGATCCCA GGATGGCAAG GGCTGCCTCC AGCGAGGCTG CCCCCCACCA CCAGCCCCCT 
CCGGAGTCCC GGGGTCTGCA AAAGAGCAGC AGCTTCTCTC AGGGCGAGGC AGAGCCCCGG 
GGTCGGCACC GCCGCGCGGG GGCACCCCTC GAAATCCCCG TGGCCAGACT TGGGGCTCGC 
AGGCTGCAGG AGTCTCCCTC CTTATCTGCC CTTAGCGAGA CCCAGCCACC GAGCCCTGCG 
AGGCCCAGCG TCCCCAAACT CAGTATCACC AAGTCCCCGG AACCTTCCGC TGTCACATCC 
AGAGACTCTC CCCAGCCCCC GGAACCTCAG CCTGTCCCAG AAAAGGTCCC TGAGCCCAAA 
CCAGAACCAG TCCGAGCTGC AAAACCAGCA CAACCCCCTT TGGCTCTGCA AATGCCAACG 
CAGCCTCTCA CCCCCTATGC CCAGATCATG CAGTCGCTCC AGCTGTCCAG TCCCACCCTG 
TCCCCACAGG ACCCCGCTGT GCCTCCATCA GAGCCCAAGC CCCACGCGGC TGTGTTTGCC 
AGGGTGGCTT CCCCACCACC CGGAGTCTCT GAGAAGCGCG TGCCTTCGGC CAGAACTCCC 
CCAGTGCTAG CCGAGAAAGC CCGAGTCCCC ACGGTGCCCC CCAGGCCAGG CAGCAGCCTC 
AGCGGCAGCA TCGAAAACCT TGAATCAGAG GCTGTGTTCG AGGCTAAGTT CAAACGCAGC 
CGCGAATCGC CACTGTCGCG GGGCCTGAGG CTACTGAGCC GCTCGCGCTC GGAGGAGCGC 
GGTCCCTTCC GTGGGGCGGA GGACGACGGC ATATACCGGC CCAGTCCGGC AGGAACGCCG 
CTAGAGCTGG TGCGACGGCC CGAGCGCTCG CGCTCCGTGC AGGACCTCAG GGTAGCCGGA 
GAGCCGGGCC TTGTGCGTCG CCTTTCGCTG TCCCTGTCGC AGAAGCTGCG GCGGACACCG 
CCGGGACAGC GACACCCTGC CTGGGAGTCC CGCAGCGGAG ACGGGGAGAG CTCCGAGGGC 
GGCAGCTCAG CTCGGGCATC CCCAGTGCTG GCGGTACGCA GGAGACTCAG CTCCACGCTG 
GAGCGTCTGT CCAGCCGTTT GCAGCGCAGC GGCAGCAGCG AGGACTCCGG GGGCGCCTCG 
GGTCGCAGCA CGCCGCTGTT TGGACGGCTG CGCAGGGCTA CTTCGGAAGG GGAGAGCCTG 
CGACGCCTCG GTGTTCCGCA CAATCAGCTG GGCTCTCAGA CTGGTGCCAC TACACCTTCC 
GCTGAGTCTC TGGGCTCAGA GGCCAGTGGC ACATCGGGTT CTTCAGCTCC TGGAGAAAGC 
CGAAGCCGGC ACCGGTGGGG CCTTTCCAGG CTGCGCAAGG ACAAGGGATT ATCTCAGCCA 
AACCTCTCCT CCAGTGTCCA AGAGGACTTG GGTCACCAGT ATGTGCCCAG TGAGTCAGAT 
TTCCCCCCAG TCTTCCACAT CAAACTCAAG GACCAGGTGC TGTTGGAGGG GGAAGCAGCC 
ACCCTGCTCT GCCTACCCGC AGCCTGCCCA GCACCCCGCA TCTCCTGGAT GAAAGATAAG 
CAGTCCCTGC GTTCAGAGCC CTCAGTGGTC ATTGTGTCCT GCAAAGATGG ACGGCAGCTG 
CTGAGCATAC CCCGGGCCGG CAAGCGGCAT GCTGGGCTCT ATGAGTGCTC AGCCACCAAT 
GTCCTGGGCA GCATTACCAG CTCCTGTACT GTGGCCGTGG CCCGCATTCC AGGGAAGCTA 
GCTCCTCCAG AGGTGCCCCA GACCTACCAT GACACAGCAC TAGTAGTGTG GAAGCCAGGA 
GACGGTCGGG CACCTTGCAC ATACACTCTG GAGCGGCGGG TGGATGGGGA ATCTGTCTGG 
CACCCTGTGA GCTCAGGCAT CCCCGACTGT TATTACAATG TGACTCAGCT GCCTGTCGGC 
GTGACCGTGC GGTTCCGTGT GGCCTGTTCC AATCGTGCTG GGCAGGGGCC CTTCAGCAAC 
CCTTCTGAGA AGGTCTTCAT CCGAGGCACT CCAGATTCTC CTGCTCAGCC AGCTGCTGCT 
CCCCGAGATG CCCCTGTTAC CTCAGGGCCA ACCAGGGCCC CACCGCCTGA CTCTCCTACC 
TCACTGGCCC CCACCCCCGC TCTTGCGCCC CCAGCCTCCC AGGCATCCAC TCTCAGCCCC 
TCAACTTCCT CCATGTCTGC CAACCAGGCC TTGTCCTCAC TCAAGGCTGT GGGTCCACCA 
CCCGCAACCC CTCCACGAAA GCACAGGGGC CTTCTGGCCA CCCAGCAAGC AGAGCCATCC 
CCACCCAGTA TCGTGGTCAC CCCAAGTGAA CCCAGGTCTT TTGTCCCTGA CACTGGAACC 
CTGACCCCAA CCTCTAGTCC TCAAGGGGTT AAACCAGCAC CTTCCTCAAC TTCCTTGTAT 
ATGGTGACTT CCTTCGTGTC TGCACCTCCA GCCCCCCAGG CCCCAGCCCC TGAGCCCCCT 
CCTGAGCCCA CCAAGGTGAC TGTGCGGAGC CTCAGCCCAG CCAAGGAGGT GGTCAGCTCC 
CCAACTCCTG AGAGCACCAC TCTTCGGCAG GGTCCCCCTC AGAAACCCTA CACCTTCCTG 
GAGGAGAAGG CCAGGGGCCG CTTTGGCGTT GTGCGGTCAT GCCGGGAGAA TGCTACGGGC 
CGAACGTTTG TCGCCAAGAT CGTGCCGTAC GCCGCGGAGG GCAAGCGACG CGTGCTGCAG 
GAATACGAGG TGCTTCGGAC GCTGCACCAC GAGCGGCTCA TGTCCCTGCA CGAGGCCTAC 
ATCACCCCTC GGTACCTCGT GCTTATCGCT GAGAGCTGTG GCAACCGGGA ACTCCTCTGT 
GGGCTCAGCG ACAGATTCCG GTATTCAGAA GATGACGTGG CCACCTATGT GGTGCAGCTG 
CTACAAGGCC TGGACTACCT CCACGGCCAC CACGTGCTAC ACCTGGACAT CAAGCCGGAC 
AATCTACTGT TGGCCGCTGA CAATGCCCTT AAAATTGTGG ACTTCGGCAG TGCTCAGCCC 
TATAACCCTC AGGCCCTGAA GCCACTTGGC CACCGCACAG GCACTCTGGA GTTCATGGCT 
CCTGAGATGG TGAAGGGAGA CCCCATTGGC TCTGCCACGG ACATCTGGGG AGCGGGTGTG 
CTCACCTACA TCATGCTTAG TGGGTACTCC CCATTCTATG AGCCAGACCC CCAGGAAACA 
GAGGCTCGGA TTGTTGGGGG TCGCTTTGAT GCCTTCCAGT TGTATCCTAA CACATCCCAG 
AGTGCCACCC TCTTCTTGAG AAAGGTCCTC TCAGTACATC CCTGGAGCCG GCCCTCTCTG 
CAGGACTGCT TGGCCCACCC ATGGCTGCAA GATGCCTACC TGATGAAGCT GCGCCGCCAG 
ACACTCACCT TCACCACCAA CCGGCTCAAG GAATTCCTGG GCGAGCAGCG GCGACGTCGG 
GCTGAGGCTG CTACCCGTCA CAAGGTGCTG CTCCGCTCCT ACCCTGGCAG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_011238650.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236952.1 Mus musculus SPEG complex locus (Speg), transcript variant X15, mRNA.

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CloneID	OMu67149
Clone ID Related Accession (Same CDS sequence)	XM_011238650.2
Accession Version	XM_011238650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
TACGACTCGG AGACTGCCGA GGATGACATC AGCGATGTGC CGGGGACCCA GCGCCTGGAG 
CTTCGGGATG ACAGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGTACC 
TCCCTGGACA CACCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG 
GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC 
CCAGGCAGCC CAAGGCAAGC ACAGACAACC GGGGCCGGGC CACGGCACCT GGGGGTGGAG 
CCGCTGGTGC GGGCATCGCG AGCTAATCTG GTGGGCGCAA GCTGGGGGTC AGAGGATAGC 
CTTTCGGTGG CCAGTGACCT CTATGGCAGC GCGTTCAGTC TGTACAGAGG ACGGGCGCTC 
TCGATCCACG TCAGCATTCC TCCGAGTGGA TTGCACAGAG AGGAGCCTGA CCTTCAGCCT 
CAGCCAGCCA GTGATGCCCT TCGTCCACGC CCTGCCCTTC CACCTCCCTC CAAATCCGCT 
TTGCTTCCCC CACCATCTCC TCGGGTGGGA AAGAGGGCTC TGCCAGGACC TAGTACCCAG 
CCCCCAGCTA CTCCCACTTC GCCCCATCGG CGTGCTCAGG AGCCCTCGCT GCCTGAGGAC 
ATCACCACCA CGGAAGAGAA GCGAGGGAAA AAGCCCAAGT CATCCGGGCC GTCGCTGGCG 
GGCACAGTGG AGTCCCGGCC CCAGACGCCA CTGAGCGAGG CTTCGGGTCG CCTGTCAGCA 
TTGGGCCGCT CGCCCCGGCT GGTGCGCGCG GGGTCCCGCA TCCTGGACAA GCTACAGTTC 
TTCGAAGAGC GGCGACGCAG CCTGGAGCGC AGCGACTCGC CGCCAGCGCC CCTGCGGCCC 
TGGGTGCCCC TGCGCAAGGC TCGCTCGCTG GAGCAGCCGA AGTCCGAGGG CGGTGCGGCG 
TGGGGCACAC CCGAGGCCTC GCAGGAGGAG CTGCGGTCAC CTCGGGGCAG TGTGGCAGAG 
CGGCGTCGCC TGTTCCAGCA AAAGGCGGCC TCGTTGGATG AACGCACGCG ACAACGCAGT 
GCAACCTCGG ACCTCGAACT CCGCTTCGCC CAGGAGCTGG GTCGCATCCG CCGATCTACG 
TCGCGGGAGG AGCTGGTGCG TTCGCACGAG TCCCTGCGTG CCACGCTGCA GCGCGCCCCA 
TCCCCTCGGG AGCCCGGCGA GCCCCCACTC TTCTCCCGGC CTTCCACACC CAAGACCTCA 
CGGGCTGTGA GCCCGGCTGC CACCCAGCCG CCGCCTCCTA GTGGTGCGGG CAAATCTGGG 
GACGAGCCTG GGAGGCCCCG AAGCAGAGGG CCGGTGGGCA GGACTGAACC GGGGGAAGGC 
CCGCAGCAGG AGATCAAGCG TCGGGACCAA TTCCCGCTAA CCAGGAGCAG AGCCATCCAG 
GAGTGCAGGA GCCCTGTGCC GCCCTACACC GCGGATCCCC CGGAGAGCAG GACAAAAGCC 
CCCTCCGGTC GCAAGCGGGA ACCCCCTGCT CAAGCGGTGC GCTTTCTGCC CTGGGCCACT 
CCGGGAGTGG AGGACTCTGT TCTGCCCCAA ACCTTGGAGA AGAATAGAGC GGGACCCGAG 
GCTGAGAAGA GGCTTCGCAG AGGACCTGAG GAGGATGGCC CCTGGGGGCC CTGGGACCGC 
AGAGGGACCC GCAGCCAAGG CAAAGGTCGC CGTGCTCGGC CTACTTCCCC CGAGCTCGAG 
TCCTCAGACG ACTCCTATGT GTCCGCTGGG GAAGAGCCCC TGGAGGCACC CGTGTTTGAG 
ATCCCTCTGC AGAATATGGT GGTGGCGCCA GGAGCTGACG TGCTACTTAA GTGTATCATC 
ACCGCCAACC CCCCACCCCA AGTGTCCTGG AAAAAGGATG GGTCCATGTT GCACAGCGAG 
GGTCGTCTTC TCATCCGGGC TGAAGGTGAA CGGCACACAC TGCTGCTCAG AGAGGCCCAG 
GCTGCCGATG CTGGGAGCTA CACAGCCACT GCCACCAACG AACTGGGCCA AGCTACCTGT 
GCTTCTTCAC TGGCTGTGAG ACCTGGCGGC TCCACATCCC CTTTCAGCAG CCCCATCACC 
TCTGATGAGG AGTACCTGAG CCCCCCAGAG GAGTTCCCAG AGCCTGGGGA GACCTGGCCC 
CGAACCCCTA CCATGAAGCT CAGTCCCAGC CAGGATCATG ATTCCTCCGA CTCTTCTTCC 
AAGGCACCCC CAACGTTCAA GGTCTCACTC ATGGACCAAT CGGTGAGAGA AGGTCAAGAT 
GTCATTATGA GCATCCGTGT GCAGGGAGAG CCCAAGCCTG TGGTTTCCTG GCTGAGGAAT 
CGACAGCCCG TGCGCCCAGA CCAGCGGCGC TTTGCAGAGG AGGCCGAGGG TGGGCTCTGC 
CGCTTGAGGA TCCTGGCTGC TGAACGGGGC GATGCTGGTT TCTACACATG CAAGGCGGTC 
AACGAATATG GCGCTCGGCA GTGCGAGGCC CGCCTGGAGG TCCGAGCACA CCCTGAAAGC 
CGGTCCCTGG CCGTGCTGGC CCCCCTGCAG GACGTGGACG TGGGGGCCGG GGAGATGGCG 
CTGTTTGAGT GCCTGGTGGC AGGTCCAGCT GACGTCGAGG TGGACTGGCT GTGCCGGGGC 
CGCCTGCTGC AGCCTGCGCT GCTCAAATGC AAGATGCATT TTGATGGCCG CAAGTGCAAG 
CTGCTGCTCA CCTCTGTGCA CGAGGACGAC AGTGGTGTCT ACACCTGCAA GCTGAGCACA 
GCCAAAGATG AACTGACCTG CAGTGCCCGG CTGACGGTTC GGCCGTCGCT GGCGCCTCTC 
TTCACCCGGT TGCTGGAAGA TGTGGAGGTG TTGGAAGGCC GTGCTGCCCG CTTGGATTGC 
AAGATCAGCG GTACCCCGCC TCCCTCCGTT ACCTGGACTC ATTTTGGCCA CCCCGTGAAT 
GAGGGTGACA ACTTGCGGCT TCAACAGGAT GGAGGTCTAC ACTCACTGCA CATTGCCCGG 
GTGGGCAGTG AGGACGAAGG ACTCTATGAA GTCAGTGCCA CCAACACTCA TGGCCAGGCC 
CACTGCTCCG CCCAGCTGTA TGTGGAGGAG CCTCGGACAG CTGCCTCTGG CCCCAGCTCG 
AAGCTGGAGA AGATGCCATC TATCCCTGAG GAGCCGGAGC ACGGAGATCT AGAGAGGCTG 
TCTATTCCTG ACTTCCTACG GCCACTGCAA GATCTGGAAG TGGGACTAGC TAAGGAGGCC 
ATGCTGGAGT GCCAAGTGAC CGGCCTGCCC TATCCCACCA TCAGCTGGTT CCACAATGGC 
CACCGCATTC AAAGCAGTGA CGACCGACGC ATGACACAGT ACAGGGACAT ACATCGCCTG 
GTGTTCCCAG CTGTGGGGCC TCAGCATGCT GGTGTCTATA AGAGTGTAAT CGCCAACAAG 
CTGGGCAAAG CGGCCTGCTA CGCCCATCTC TATGTCACAG ATGTGGTTCC AGGCCCTCCA 
GATGGTGCTC CGGAAGTAGT AGCTGTGACC GGAAGGATGG TCACACTATC GTGGAACCCA 
CCCAGGAGTC TGGACATGGC TATTGACCCA GATTCCCTAA CATACACTGT GCAGCACCAG 
GTGTTGGGCT CGGACCAGTG GACGGCACTG GTCACAGGCC TGCGGGAGCC TGCGTGGGCA 
GCCACAGGGC TGAAGAAGGG GATCCAGCAC ATCTTCCGGG TCCTCAGCTC CAGTGGCAAG 
AGCAGCAGCA AGCCCTCAGC ACCGTCGGAA CCCGTGCAGC TGCTAGAGCA TGGCCCACCC 
CTGGAAGAGG CGCCTGCTGT GCTGGACAAG CAGGACATTG TGTATGTGGT AGAGGGCCAG 
CCTGCCTGTG TCACCGTCAC TTTTAACCAT GTGGAGGCCC AGGTTGTCTG GAGGAGCTGC 
CGGGGAGCCC TCCTCGAGGC GCGCACAGGT GTGTATGAAC TGAGTCAGCC AGATGACGAC 
CAGTATTGTC TGCGGATCTG CCGGGTGAGC CGACGAGACC TGGGACCCCT TACTTGCAGT 
GCCCGAAACC GCCACGGCAC AAAGGCCTGC TCGGTCACCC TGGAGCTGGC AGAGGCACCT 
CGGTTTGAGT CTATCATGGA AGATGTGGAG GTGGGGCCTG GAGAAACCGC TCGCTTTGCT 
GTGGTAGTTG AGGGGAAACC ACTGCCAGAC ATCATGTGGT ACAAGGATGA GGTGCTGCTG 
GCCGAGAGCA ACCATGTGAG CTTCGTGTAT GAGGAGAACG AGTGTTCCCT AGTGCTGCTC 
AGCGCAGGAA GCCAGGATGG AGGCGTCTAT ACCTGTACCG CCCGGAACCT GGCAGGCGAA 
GTCTCCTGCA AGGCGGAGCT GTCTGTGCTT TCAGCTCAGA CAGCCATGGA GGTCGAGGGG 
GTCGGAGAGG ATGAGGAACA CCGAGGAAGA AGACTTAGCG ACTACTACGA CATCCACCAA 
GAGATTGGCA GGGGTGCCTT CTCCTACCTG CGGCGTGTAG TGGAGAGGAG CTCTGGCCTG 
GAGTTTGCAG CCAAGTTCAT CCCTAGCCAG GCTAAGCCTA AAGCATCAGC AAGACGCGAG 
GCGCGCTTGC TGGCCCGGCT CCAGCATGGC TGTGTGCTCT ACTTCCACGA AGCCTTTGAG 
AGGCGCAGGG GACTTGTCAT AGTCACGGAG CTCTGCACAG AAGAGCTTCT AGAGCGCATG 
GCCAGGAAGC CCACCGTGTA CTCGGACCTA TATGCGTCAA GTGCTAGAGG GGATATGCTA 
TCTGCATCAG AGCCATGTGC TGCACCTGGA TGTCAAGCCG GAGAACCTGC TTGTGTGGGA 
TGGTGCAGGG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236952.1
CDS	106..5340
Translation	MQKARGTRGEDAGTRAPPSPGVPPKRAKVGAGRGVLVTGDGAGAPVFLRPLKNAAVCAGSDVRLRVVVSGTPQPSLSWFRDGQLLPPPAPEPSCLWLRSCGAQDAGVYSCSAQNERGQASCEAVLTVLEVRVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPRQAQTTGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVYSDLYASSARGDMLSASEPCAAPGCQAGEPACVGWCRG

Target ORF information:

RefSeq Version	XM_011238650.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238650.2

ORF Insert Sequence:

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
TACGACTCGG AGACTGCCGA GGATGACATC AGCGATGTGC CGGGGACCCA GCGCCTGGAG 
CTTCGGGATG ACAGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGTACC 
TCCCTGGACA CACCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG 
GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC 
CCAGGCAGCC CAAGGCAAGC ACAGACAACC GGGGCCGGGC CACGGCACCT GGGGGTGGAG 
CCGCTGGTGC GGGCATCGCG AGCTAATCTG GTGGGCGCAA GCTGGGGGTC AGAGGATAGC 
CTTTCGGTGG CCAGTGACCT CTATGGCAGC GCGTTCAGTC TGTACAGAGG ACGGGCGCTC 
TCGATCCACG TCAGCATTCC TCCGAGTGGA TTGCACAGAG AGGAGCCTGA CCTTCAGCCT 
CAGCCAGCCA GTGATGCCCT TCGTCCACGC CCTGCCCTTC CACCTCCCTC CAAATCCGCT 
TTGCTTCCCC CACCATCTCC TCGGGTGGGA AAGAGGGCTC TGCCAGGACC TAGTACCCAG 
CCCCCAGCTA CTCCCACTTC GCCCCATCGG CGTGCTCAGG AGCCCTCGCT GCCTGAGGAC 
ATCACCACCA CGGAAGAGAA GCGAGGGAAA AAGCCCAAGT CATCCGGGCC GTCGCTGGCG 
GGCACAGTGG AGTCCCGGCC CCAGACGCCA CTGAGCGAGG CTTCGGGTCG CCTGTCAGCA 
TTGGGCCGCT CGCCCCGGCT GGTGCGCGCG GGGTCCCGCA TCCTGGACAA GCTACAGTTC 
TTCGAAGAGC GGCGACGCAG CCTGGAGCGC AGCGACTCGC CGCCAGCGCC CCTGCGGCCC 
TGGGTGCCCC TGCGCAAGGC TCGCTCGCTG GAGCAGCCGA AGTCCGAGGG CGGTGCGGCG 
TGGGGCACAC CCGAGGCCTC GCAGGAGGAG CTGCGGTCAC CTCGGGGCAG TGTGGCAGAG 
CGGCGTCGCC TGTTCCAGCA AAAGGCGGCC TCGTTGGATG AACGCACGCG ACAACGCAGT 
GCAACCTCGG ACCTCGAACT CCGCTTCGCC CAGGAGCTGG GTCGCATCCG CCGATCTACG 
TCGCGGGAGG AGCTGGTGCG TTCGCACGAG TCCCTGCGTG CCACGCTGCA GCGCGCCCCA 
TCCCCTCGGG AGCCCGGCGA GCCCCCACTC TTCTCCCGGC CTTCCACACC CAAGACCTCA 
CGGGCTGTGA GCCCGGCTGC CACCCAGCCG CCGCCTCCTA GTGGTGCGGG CAAATCTGGG 
GACGAGCCTG GGAGGCCCCG AAGCAGAGGG CCGGTGGGCA GGACTGAACC GGGGGAAGGC 
CCGCAGCAGG AGATCAAGCG TCGGGACCAA TTCCCGCTAA CCAGGAGCAG AGCCATCCAG 
GAGTGCAGGA GCCCTGTGCC GCCCTACACC GCGGATCCCC CGGAGAGCAG GACAAAAGCC 
CCCTCCGGTC GCAAGCGGGA ACCCCCTGCT CAAGCGGTGC GCTTTCTGCC CTGGGCCACT 
CCGGGAGTGG AGGACTCTGT TCTGCCCCAA ACCTTGGAGA AGAATAGAGC GGGACCCGAG 
GCTGAGAAGA GGCTTCGCAG AGGACCTGAG GAGGATGGCC CCTGGGGGCC CTGGGACCGC 
AGAGGGACCC GCAGCCAAGG CAAAGGTCGC CGTGCTCGGC CTACTTCCCC CGAGCTCGAG 
TCCTCAGACG ACTCCTATGT GTCCGCTGGG GAAGAGCCCC TGGAGGCACC CGTGTTTGAG 
ATCCCTCTGC AGAATATGGT GGTGGCGCCA GGAGCTGACG TGCTACTTAA GTGTATCATC 
ACCGCCAACC CCCCACCCCA AGTGTCCTGG AAAAAGGATG GGTCCATGTT GCACAGCGAG 
GGTCGTCTTC TCATCCGGGC TGAAGGTGAA CGGCACACAC TGCTGCTCAG AGAGGCCCAG 
GCTGCCGATG CTGGGAGCTA CACAGCCACT GCCACCAACG AACTGGGCCA AGCTACCTGT 
GCTTCTTCAC TGGCTGTGAG ACCTGGCGGC TCCACATCCC CTTTCAGCAG CCCCATCACC 
TCTGATGAGG AGTACCTGAG CCCCCCAGAG GAGTTCCCAG AGCCTGGGGA GACCTGGCCC 
CGAACCCCTA CCATGAAGCT CAGTCCCAGC CAGGATCATG ATTCCTCCGA CTCTTCTTCC 
AAGGCACCCC CAACGTTCAA GGTCTCACTC ATGGACCAAT CGGTGAGAGA AGGTCAAGAT 
GTCATTATGA GCATCCGTGT GCAGGGAGAG CCCAAGCCTG TGGTTTCCTG GCTGAGGAAT 
CGACAGCCCG TGCGCCCAGA CCAGCGGCGC TTTGCAGAGG AGGCCGAGGG TGGGCTCTGC 
CGCTTGAGGA TCCTGGCTGC TGAACGGGGC GATGCTGGTT TCTACACATG CAAGGCGGTC 
AACGAATATG GCGCTCGGCA GTGCGAGGCC CGCCTGGAGG TCCGAGCACA CCCTGAAAGC 
CGGTCCCTGG CCGTGCTGGC CCCCCTGCAG GACGTGGACG TGGGGGCCGG GGAGATGGCG 
CTGTTTGAGT GCCTGGTGGC AGGTCCAGCT GACGTCGAGG TGGACTGGCT GTGCCGGGGC 
CGCCTGCTGC AGCCTGCGCT GCTCAAATGC AAGATGCATT TTGATGGCCG CAAGTGCAAG 
CTGCTGCTCA CCTCTGTGCA CGAGGACGAC AGTGGTGTCT ACACCTGCAA GCTGAGCACA 
GCCAAAGATG AACTGACCTG CAGTGCCCGG CTGACGGTTC GGCCGTCGCT GGCGCCTCTC 
TTCACCCGGT TGCTGGAAGA TGTGGAGGTG TTGGAAGGCC GTGCTGCCCG CTTGGATTGC 
AAGATCAGCG GTACCCCGCC TCCCTCCGTT ACCTGGACTC ATTTTGGCCA CCCCGTGAAT 
GAGGGTGACA ACTTGCGGCT TCAACAGGAT GGAGGTCTAC ACTCACTGCA CATTGCCCGG 
GTGGGCAGTG AGGACGAAGG ACTCTATGAA GTCAGTGCCA CCAACACTCA TGGCCAGGCC 
CACTGCTCCG CCCAGCTGTA TGTGGAGGAG CCTCGGACAG CTGCCTCTGG CCCCAGCTCG 
AAGCTGGAGA AGATGCCATC TATCCCTGAG GAGCCGGAGC ACGGAGATCT AGAGAGGCTG 
TCTATTCCTG ACTTCCTACG GCCACTGCAA GATCTGGAAG TGGGACTAGC TAAGGAGGCC 
ATGCTGGAGT GCCAAGTGAC CGGCCTGCCC TATCCCACCA TCAGCTGGTT CCACAATGGC 
CACCGCATTC AAAGCAGTGA CGACCGACGC ATGACACAGT ACAGGGACAT ACATCGCCTG 
GTGTTCCCAG CTGTGGGGCC TCAGCATGCT GGTGTCTATA AGAGTGTAAT CGCCAACAAG 
CTGGGCAAAG CGGCCTGCTA CGCCCATCTC TATGTCACAG ATGTGGTTCC AGGCCCTCCA 
GATGGTGCTC CGGAAGTAGT AGCTGTGACC GGAAGGATGG TCACACTATC GTGGAACCCA 
CCCAGGAGTC TGGACATGGC TATTGACCCA GATTCCCTAA CATACACTGT GCAGCACCAG 
GTGTTGGGCT CGGACCAGTG GACGGCACTG GTCACAGGCC TGCGGGAGCC TGCGTGGGCA 
GCCACAGGGC TGAAGAAGGG GATCCAGCAC ATCTTCCGGG TCCTCAGCTC CAGTGGCAAG 
AGCAGCAGCA AGCCCTCAGC ACCGTCGGAA CCCGTGCAGC TGCTAGAGCA TGGCCCACCC 
CTGGAAGAGG CGCCTGCTGT GCTGGACAAG CAGGACATTG TGTATGTGGT AGAGGGCCAG 
CCTGCCTGTG TCACCGTCAC TTTTAACCAT GTGGAGGCCC AGGTTGTCTG GAGGAGCTGC 
CGGGGAGCCC TCCTCGAGGC GCGCACAGGT GTGTATGAAC TGAGTCAGCC AGATGACGAC 
CAGTATTGTC TGCGGATCTG CCGGGTGAGC CGACGAGACC TGGGACCCCT TACTTGCAGT 
GCCCGAAACC GCCACGGCAC AAAGGCCTGC TCGGTCACCC TGGAGCTGGC AGAGGCACCT 
CGGTTTGAGT CTATCATGGA AGATGTGGAG GTGGGGCCTG GAGAAACCGC TCGCTTTGCT 
GTGGTAGTTG AGGGGAAACC ACTGCCAGAC ATCATGTGGT ACAAGGATGA GGTGCTGCTG 
GCCGAGAGCA ACCATGTGAG CTTCGTGTAT GAGGAGAACG AGTGTTCCCT AGTGCTGCTC 
AGCGCAGGAA GCCAGGATGG AGGCGTCTAT ACCTGTACCG CCCGGAACCT GGCAGGCGAA 
GTCTCCTGCA AGGCGGAGCT GTCTGTGCTT TCAGCTCAGA CAGCCATGGA GGTCGAGGGG 
GTCGGAGAGG ATGAGGAACA CCGAGGAAGA AGACTTAGCG ACTACTACGA CATCCACCAA 
GAGATTGGCA GGGGTGCCTT CTCCTACCTG CGGCGTGTAG TGGAGAGGAG CTCTGGCCTG 
GAGTTTGCAG CCAAGTTCAT CCCTAGCCAG GCTAAGCCTA AAGCATCAGC AAGACGCGAG 
GCGCGCTTGC TGGCCCGGCT CCAGCATGGC TGTGTGCTCT ACTTCCACGA AGCCTTTGAG 
AGGCGCAGGG GACTTGTCAT AGTCACGGAG CTCTGCACAG AAGAGCTTCT AGAGCGCATG 
GCCAGGAAGC CCACCGTGTA CTCGGACCTA TATGCGTCAA GTGCTAGAGG GGATATGCTA 
TCTGCATCAG AGCCATGTGC TGCACCTGGA TGTCAAGCCG GAGAACCTGC TTGTGTGGGA 
TGGTGCAGGG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_030243284.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099144.1 Mus musculus SPEG complex locus (Speg), transcript variant X12, mRNA.

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CloneID	OMu20941
Clone ID Related Accession (Same CDS sequence)	XM_006496400.2 , XM_011238648.2 , XM_030243284.1 , XM_006496401.2 , XM_011238649.2 , XM_011238649.3 , NM_001085370.1 , XM_011238648.3 , XM_030243273.1
Accession Version	XM_030243284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099144.1
CDS	1138..8721
Translation	MVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_030243284.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243284.1

ORF Insert Sequence:

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_011238649.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236951.1 Mus musculus SPEG complex locus (Speg), transcript variant X10, mRNA.

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CloneID	OMu20941
Clone ID Related Accession (Same CDS sequence)	XM_006496400.2 , XM_011238648.2 , XM_030243284.1 , XM_006496401.2 , XM_011238649.2 , XM_011238649.3 , NM_001085370.1 , XM_011238648.3 , XM_030243273.1
Accession Version	XM_011238649.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238649.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236951.1
CDS	1748..9331
Translation	MVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_011238649.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238649.3

ORF Insert Sequence:

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_011238648.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236950.1 Mus musculus SPEG complex locus (Speg), transcript variant X9, mRNA.

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CloneID	OMu20941
Clone ID Related Accession (Same CDS sequence)	XM_006496400.2 , XM_011238648.2 , XM_030243284.1 , XM_006496401.2 , XM_011238649.2 , XM_011238649.3 , NM_001085370.1 , XM_011238648.3 , XM_030243273.1
Accession Version	XM_011238648.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238648.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236950.1
CDS	2300..9883
Translation	MVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_011238648.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238648.3

ORF Insert Sequence:

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_030243273.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099133.1 Mus musculus SPEG complex locus (Speg), transcript variant X11, mRNA.

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CloneID	OMu20941
Clone ID Related Accession (Same CDS sequence)	XM_006496400.2 , XM_011238648.2 , XM_030243284.1 , XM_006496401.2 , XM_011238649.2 , XM_011238649.3 , NM_001085370.1 , XM_011238648.3 , XM_030243273.1
Accession Version	XM_030243273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099133.1
CDS	3347..10930
Translation	MVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_030243273.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243273.1

ORF Insert Sequence:

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_006496404.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496467.1 Mus musculus SPEG complex locus (Speg), transcript variant X16, mRNA.

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CloneID	OMu27706
Clone ID Related Accession (Same CDS sequence)	XM_006496404.4 , XM_006496404.3 , XM_006496403.3 , XM_006496403.4
Accession Version	XM_006496404.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496404.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCA GTCCCAGCCA GGATCATGAT TCCTCCGACT CTTCTTCCAA GGCACCCCCA 
ACGTTCAAGG TCTCACTCAT GGACCAATCG GTGAGAGAAG GTCAAGATGT CATTATGAGC 
ATCCGTGTGC AGGGAGAGCC CAAGCCTGTG GTTTCCTGGC TGAGGAATCG ACAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCAGAGGAG GCCGAGGGTG GGCTCTGCCG CTTGAGGATC 
CTGGCTGCTG AACGGGGCGA TGCTGGTTTC TACACATGCA AGGCGGTCAA CGAATATGGC 
GCTCGGCAGT GCGAGGCCCG CCTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCAG GTCCAGCTGA CGTCGAGGTG GACTGGCTGT GCCGGGGCCG CCTGCTGCAG 
CCTGCGCTGC TCAAATGCAA GATGCATTTT GATGGCCGCA AGTGCAAGCT GCTGCTCACC 
TCTGTGCACG AGGACGACAG TGGTGTCTAC ACCTGCAAGC TGAGCACAGC CAAAGATGAA 
CTGACCTGCA GTGCCCGGCT GACGGTTCGG CCGTCGCTGG CGCCTCTCTT CACCCGGTTG 
CTGGAAGATG TGGAGGTGTT GGAAGGCCGT GCTGCCCGCT TGGATTGCAA GATCAGCGGT 
ACCCCGCCTC CCTCCGTTAC CTGGACTCAT TTTGGCCACC CCGTGAATGA GGGTGACAAC 
TTGCGGCTTC AACAGGATGG AGGTCTACAC TCACTGCACA TTGCCCGGGT GGGCAGTGAG 
GACGAAGGAC TCTATGAAGT CAGTGCCACC AACACTCATG GCCAGGCCCA CTGCTCCGCC 
CAGCTGTATG TGGAGGAGCC TCGGACAGCT GCCTCTGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCTA TCCCTGAGGA GCCGGAGCAC GGAGATCTAG AGAGGCTGTC TATTCCTGAC 
TTCCTACGGC CACTGCAAGA TCTGGAAGTG GGACTAGCTA AGGAGGCCAT GCTGGAGTGC 
CAAGTGACCG GCCTGCCCTA TCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATTCAA 
AGCAGTGACG ACCGACGCAT GACACAGTAC AGGGACATAC ATCGCCTGGT GTTCCCAGCT 
GTGGGGCCTC AGCATGCTGG TGTCTATAAG AGTGTAATCG CCAACAAGCT GGGCAAAGCG 
GCCTGCTACG CCCATCTCTA TGTCACAGAT GTGGTTCCAG GCCCTCCAGA TGGTGCTCCG 
GAAGTAGTAG CTGTGACCGG AAGGATGGTC ACACTATCGT GGAACCCACC CAGGAGTCTG 
GACATGGCTA TTGACCCAGA TTCCCTAACA TACACTGTGC AGCACCAGGT GTTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCTG CGTGGGCAGC CACAGGGCTG 
AAGAAGGGGA TCCAGCACAT CTTCCGGGTC CTCAGCTCCA GTGGCAAGAG CAGCAGCAAG 
CCCTCAGCAC CGTCGGAACC CGTGCAGCTG CTAGAGCATG GCCCACCCCT GGAAGAGGCG 
CCTGCTGTGC TGGACAAGCA GGACATTGTG TATGTGGTAG AGGGCCAGCC TGCCTGTGTC 
ACCGTCACTT TTAACCATGT GGAGGCCCAG GTTGTCTGGA GGAGCTGCCG GGGAGCCCTC 
CTCGAGGCGC GCACAGGTGT GTATGAACTG AGTCAGCCAG ATGACGACCA GTATTGTCTG 
CGGATCTGCC GGGTGAGCCG ACGAGACCTG GGACCCCTTA CTTGCAGTGC CCGAAACCGC 
CACGGCACAA AGGCCTGCTC GGTCACCCTG GAGCTGGCAG AGGCACCTCG GTTTGAGTCT 
ATCATGGAAG ATGTGGAGGT GGGGCCTGGA GAAACCGCTC GCTTTGCTGT GGTAGTTGAG 
GGGAAACCAC TGCCAGACAT CATGTGGTAC AAGGATGAGG TGCTGCTGGC CGAGAGCAAC 
CATGTGAGCT TCGTGTATGA GGAGAACGAG TGTTCCCTAG TGCTGCTCAG CGCAGGAAGC 
CAGGATGGAG GCGTCTATAC CTGTACCGCC CGGAACCTGG CAGGCGAAGT CTCCTGCAAG 
GCGGAGCTGT CTGTGCTTTC AGCTCAGACA GCCATGGAGG TCGAGGGGGT CGGAGAGGAT 
GAGGAACACC GAGGAAGAAG ACTTAGCGAC TACTACGACA TCCACCAAGA GATTGGCAGG 
GGTGCCTTCT CCTACCTGCG GCGTGTAGTG GAGAGGAGCT CTGGCCTGGA GTTTGCAGCC 
AAGTTCATCC CTAGCCAGGC TAAGCCTAAA GCATCAGCAA GACGCGAGGC GCGCTTGCTG 
GCCCGGCTCC AGCATGGCTG TGTGCTCTAC TTCCACGAAG CCTTTGAGAG GCGCAGGGGA 
CTTGTCATAG TCACGGAGCT CTGCACAGAA GAGCTTCTAG AGCGCATGGC CAGGAAGCCC 
ACCGTGTGTG AGTCTGAGAC TCGGACCTAT ATGCGTCAAG TGCTAGAGGG GATATGCTAT 
CTGCATCAGA GCCATGTGCT GCACCTGGAT GTCAAGCCGG AGAACCTGCT TGTGTGGGAT 
GGTGCAGGGG GTGAAGAGCA GGTGCGCATC TGTGACTTCG GGAACGCCCA GGAACTCACT 
CCGGGAGAGC CCCAGTACTG TCAGTATGGC ACGCCAGAGT TTGTAGCACC TGAGATTGTC 
AACCAGAGCC CTGTGTCTGG AGTCACTGAC ATCTGGCCCG TGGGCGTCGT TGCCTTTCTC 
TGTTTGACGG GCATCTCCCC ATTTGTTGGG GAGAATGACC GGACAACACT CATGAATATC 
CGAAACTACA ACGTGGCATT TGAGGAGACT ACGTTCCTGA GTCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGCT GGTGCAGGAT CGACTGAGAC CTACTGCAGA AGAGACATTG 
GAACATCCTT GGTTCAAAAC AGAAGCAAAG GGTGCAGAGG TGAGCACAGA TCACCTGAAG 
CTCTTCCTGT CACGGAGGAG GTGGCAGCGC TCTCAAATCA GCTACAAGTG CCACCTGGTG 
CTGCGCCCCA TTCCGGAACT GCTACGGGCT CCCCCGGAGC GGGTGTGGGT GGCCATGCCT 
AGAAGACAAC CCCCGAGTGG GGGACTCTCA TCCTCTTCAG ACTCAGAAGA GGAAGAGTTG 
GAGGAACTTC CCTCAGTACC CCGCCCCCTG CAGCCTGAGT TCTCAGGTTC TCGGGTATCC 
CTCACCGACA TCCCCACCGA AGATGAGGCT CTGGGGACCC CGGAAGCTGG GGCTGCCACC 
CCCATGGACT GGCAAGAGCA GGAAAGGACT CCCTCTAAGG ACCAGGAGGC CCCCAGCCCT 
GAGGCACTCC CCTCTCCAGG CCAGGAGTCC CCTGATGGGC CTAGCCCCAG GCGGCCAGAG 
CTCCGCAGGG GTAGCTCTGC TGAGAGTGCC CTGCCCCGAG TCGGGTCGCG GGAACCGGGC 
CGGAGCTTGC ACAAGGCAGC ATCCGTGGAG CTGCCGCAGC GCAGGAGCCC CAGCCCGGGT 
GCCACCCGCC TGACTCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGTCAGC GGCTGCTGAG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGA 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCA CGGGATCCCA GGATGGCAAG GGCTGCCTCC 
AGCGAGGCTG CCCCCCACCA CCAGCCCCCT CCGGAGTCCC GGGGTCTGCA AAAGAGCAGC 
AGCTTCTCTC AGGGCGAGGC AGAGCCCCGG GGTCGGCACC GCCGCGCGGG GGCACCCCTC 
GAAATCCCCG TGGCCAGACT TGGGGCTCGC AGGCTGCAGG AGTCTCCCTC CTTATCTGCC 
CTTAGCGAGA CCCAGCCACC GAGCCCTGCG AGGCCCAGCG TCCCCAAACT CAGTATCACC 
AAGTCCCCGG AACCTTCCGC TGTCACATCC AGAGACTCTC CCCAGCCCCC GGAACCTCAG 
CCTGTCCCAG AAAAGGTCCC TGAGCCCAAA CCAGAACCAG TCCGAGCTGC AAAACCAGCA 
CAACCCCCTT TGGCTCTGCA AATGCCAACG CAGCCTCTCA CCCCCTATGC CCAGATCATG 
CAGTCGCTCC AGCTGTCCAG TCCCACCCTG TCCCCACAGG ACCCCGCTGT GCCTCCATCA 
GAGCCCAAGC CCCACGCGGC TGTGTTTGCC AGGGTGGCTT CCCCACCACC CGGAGTCTCT 
GAGAAGCGCG TGCCTTCGGC CAGAACTCCC CCAGTGCTAG CCGAGAAAGC CCGAGTCCCC 
ACGGTGCCCC CCAGGCCAGG CAGCAGCCTC AGCGGCAGCA TCGAAAACCT TGAATCAGAG 
GCTGTGTTCG AGGCTAAGTT CAAACGCAGC CGCGAATCGC CACTGTCGCG GGGCCTGAGG 
CTACTGAGCC GCTCGCGCTC GGAGGAGCGC GGTCCCTTCC GTGGGGCGGA GGACGACGGC 
ATATACCGGC CCAGTCCGGC AGGAACGCCG CTAGAGCTGG TGCGACGGCC CGAGCGCTCG 
CGCTCCGTGC AGGACCTCAG GGTAGCCGGA GAGCCGGGCC TTGTGCGTCG CCTTTCGCTG 
TCCCTGTCGC AGAAGCTGCG GCGGACACCG CCGGGACAGC GACACCCTGC CTGGGAGTCC 
CGCAGCGGAG ACGGGGAGAG CTCCGAGGGC GGCAGCTCAG CTCGGGCATC CCCAGTGCTG 
GCGGTACGCA GGAGACTCAG CTCCACGCTG GAGCGTCTGT CCAGCCGTTT GCAGCGCAGC 
GGCAGCAGCG AGGACTCCGG GGGCGCCTCG GGTCGCAGCA CGCCGCTGTT TGGACGGCTG 
CGCAGGGCTA CTTCGGAAGG GGAGAGCCTG CGACGCCTCG GTGTTCCGCA CAATCAGCTG 
GGCTCTCAGA CTGGTGCCAC TACACCTTCC GCTGAGTCTC TGGGCTCAGA GGCCAGTGGC 
ACATCGGGTT CTTCAGCTCC TGGAGAAAGC CGAAGCCGGC ACCGGTGGGG CCTTTCCAGG 
CTGCGCAAGG ACAAGGGATT ATCTCAGCCA AACCTCTCCT CCAGTGTCCA AGAGGACTTG 
GGTCACCAGT ATGTGCCCAG TGAGTCAGAT TTCCCCCCAG TCTTCCACAT CAAACTCAAG 
GACCAGGTGC TGTTGGAGGG GGAAGCAGCC ACCCTGCTCT GCCTACCCGC AGCCTGCCCA 
GCACCCCGCA TCTCCTGGAT GAAAGATAAG CAGTCCCTGC GTTCAGAGCC CTCAGTGGTC 
ATTGTGTCCT GCAAAGATGG ACGGCAGCTG CTGAGCATAC CCCGGGCCGG CAAGCGGCAT 
GCTGGGCTCT ATGAGTGCTC AGCCACCAAT GTCCTGGGCA GCATTACCAG CTCCTGTACT 
GTGGCCGTGG CCCGCATTCC AGGGAAGCTA GCTCCTCCAG AGGTGCCCCA GACCTACCAT 
GACACAGCAC TAGTAGTGTG GAAGCCAGGA GACGGTCGGG CACCTTGCAC ATACACTCTG 
GAGCGGCGGG TGGATGGGGA ATCTGTCTGG CACCCTGTGA GCTCAGGCAT CCCCGACTGT 
TATTACAATG TGACTCAGCT GCCTGTCGGC GTGACCGTGC GGTTCCGTGT GGCCTGTTCC 
AATCGTGCTG GGCAGGGGCC CTTCAGCAAC CCTTCTGAGA AGGTCTTCAT CCGAGGCACT 
CCAGATTCTC CTGCTCAGCC AGCTGCTGCT CCCCGAGATG CCCCTGTTAC CTCAGGGCCA 
ACCAGGGCCC CACCGCCTGA CTCTCCTACC TCACTGGCCC CCACCCCCGC TCTTGCGCCC 
CCAGCCTCCC AGGCATCCAC TCTCAGCCCC TCAACTTCCT CCATGTCTGC CAACCAGGCC 
TTGTCCTCAC TCAAGGCTGT GGGTCCACCA CCCGCAACCC CTCCACGAAA GCACAGGGGC 
CTTCTGGCCA CCCAGCAAGC AGAGCCATCC CCACCCAGTA TCGTGGTCAC CCCAAGTGAA 
CCCAGGTCTT TTGTCCCTGA CACTGGAACC CTGACCCCAA CCTCTAGTCC TCAAGGGGTT 
AAACCAGCAC CTTCCTCAAC TTCCTTGTAT ATGGTGACTT CCTTCGTGTC TGCACCTCCA 
GCCCCCCAGG CCCCAGCCCC TGAGCCCCCT CCTGAGCCCA CCAAGGTGAC TGTGCGGAGC 
CTCAGCCCAG CCAAGGAGGT GGTCAGCTCC CCAACTCCTG AGAGCACCAC TCTTCGGCAG 
GGTCCCCCTC AGAAACCCTA CACCTTCCTG GAGGAGAAGG CCAGGGGCCG CTTTGGCGTT 
GTGCGGTCAT GCCGGGAGAA TGCTACGGGC CGAACGTTTG TCGCCAAGAT CGTGCCGTAC 
GCCGCGGAGG GCAAGCGACG CGTGCTGCAG GAATACGAGG TGCTTCGGAC GCTGCACCAC 
GAGCGGCTCA TGTCCCTGCA CGAGGCCTAC ATCACCCCTC GGTACCTCGT GCTTATCGCT 
GAGAGCTGTG GCAACCGGGA ACTCCTCTGT GGGCTCAGCG ACAGATTCCG GTATTCAGAA 
GATGACGTGG CCACCTATGT GGTGCAGCTG CTACAAGGCC TGGACTACCT CCACGGCCAC 
CACGTGCTAC ACCTGGACAT CAAGCCGGAC AATCTACTGT TGGCCGCTGA CAATGCCCTT 
AAAATTGTGG ACTTCGGCAG TGCTCAGCCC TATAACCCTC AGGCCCTGAA GCCACTTGGC 
CACCGCACAG GCACTCTGGA GTTCATGGCT CCTGAGATGG TGAAGGGAGA CCCCATTGGC 
TCTGCCACGG ACATCTGGGG AGCGGGTGTG CTCACCTACA TCATGCTTAG TGGGTACTCC 
CCATTCTATG AGCCAGACCC CCAGGAAACA GAGGCTCGGA TTGTTGGGGG TCGCTTTGAT 
GCCTTCCAGT TGTATCCTAA CACATCCCAG AGTGCCACCC TCTTCTTGAG AAAGGTCCTC 
TCAGTACATC CCTGGAGCCG GCCCTCTCTG CAGGACTGCT TGGCCCACCC ATGGCTGCAA 
GATGCCTACC TGATGAAGCT GCGCCGCCAG ACACTCACCT TCACCACCAA CCGGCTCAAG 
GAATTCCTGG GCGAGCAGCG GCGACGTCGG GCTGAGGCTG CTACCCGTCA CAAGGTGCTG 
CTCCGCTCCT ACCCTGGCAG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496467.1
CDS	206..7432
Translation	MKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_006496404.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496404.4

ORF Insert Sequence:

ATGAAGCTCA GTCCCAGCCA GGATCATGAT TCCTCCGACT CTTCTTCCAA GGCACCCCCA 
ACGTTCAAGG TCTCACTCAT GGACCAATCG GTGAGAGAAG GTCAAGATGT CATTATGAGC 
ATCCGTGTGC AGGGAGAGCC CAAGCCTGTG GTTTCCTGGC TGAGGAATCG ACAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCAGAGGAG GCCGAGGGTG GGCTCTGCCG CTTGAGGATC 
CTGGCTGCTG AACGGGGCGA TGCTGGTTTC TACACATGCA AGGCGGTCAA CGAATATGGC 
GCTCGGCAGT GCGAGGCCCG CCTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCAG GTCCAGCTGA CGTCGAGGTG GACTGGCTGT GCCGGGGCCG CCTGCTGCAG 
CCTGCGCTGC TCAAATGCAA GATGCATTTT GATGGCCGCA AGTGCAAGCT GCTGCTCACC 
TCTGTGCACG AGGACGACAG TGGTGTCTAC ACCTGCAAGC TGAGCACAGC CAAAGATGAA 
CTGACCTGCA GTGCCCGGCT GACGGTTCGG CCGTCGCTGG CGCCTCTCTT CACCCGGTTG 
CTGGAAGATG TGGAGGTGTT GGAAGGCCGT GCTGCCCGCT TGGATTGCAA GATCAGCGGT 
ACCCCGCCTC CCTCCGTTAC CTGGACTCAT TTTGGCCACC CCGTGAATGA GGGTGACAAC 
TTGCGGCTTC AACAGGATGG AGGTCTACAC TCACTGCACA TTGCCCGGGT GGGCAGTGAG 
GACGAAGGAC TCTATGAAGT CAGTGCCACC AACACTCATG GCCAGGCCCA CTGCTCCGCC 
CAGCTGTATG TGGAGGAGCC TCGGACAGCT GCCTCTGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCTA TCCCTGAGGA GCCGGAGCAC GGAGATCTAG AGAGGCTGTC TATTCCTGAC 
TTCCTACGGC CACTGCAAGA TCTGGAAGTG GGACTAGCTA AGGAGGCCAT GCTGGAGTGC 
CAAGTGACCG GCCTGCCCTA TCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATTCAA 
AGCAGTGACG ACCGACGCAT GACACAGTAC AGGGACATAC ATCGCCTGGT GTTCCCAGCT 
GTGGGGCCTC AGCATGCTGG TGTCTATAAG AGTGTAATCG CCAACAAGCT GGGCAAAGCG 
GCCTGCTACG CCCATCTCTA TGTCACAGAT GTGGTTCCAG GCCCTCCAGA TGGTGCTCCG 
GAAGTAGTAG CTGTGACCGG AAGGATGGTC ACACTATCGT GGAACCCACC CAGGAGTCTG 
GACATGGCTA TTGACCCAGA TTCCCTAACA TACACTGTGC AGCACCAGGT GTTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCTG CGTGGGCAGC CACAGGGCTG 
AAGAAGGGGA TCCAGCACAT CTTCCGGGTC CTCAGCTCCA GTGGCAAGAG CAGCAGCAAG 
CCCTCAGCAC CGTCGGAACC CGTGCAGCTG CTAGAGCATG GCCCACCCCT GGAAGAGGCG 
CCTGCTGTGC TGGACAAGCA GGACATTGTG TATGTGGTAG AGGGCCAGCC TGCCTGTGTC 
ACCGTCACTT TTAACCATGT GGAGGCCCAG GTTGTCTGGA GGAGCTGCCG GGGAGCCCTC 
CTCGAGGCGC GCACAGGTGT GTATGAACTG AGTCAGCCAG ATGACGACCA GTATTGTCTG 
CGGATCTGCC GGGTGAGCCG ACGAGACCTG GGACCCCTTA CTTGCAGTGC CCGAAACCGC 
CACGGCACAA AGGCCTGCTC GGTCACCCTG GAGCTGGCAG AGGCACCTCG GTTTGAGTCT 
ATCATGGAAG ATGTGGAGGT GGGGCCTGGA GAAACCGCTC GCTTTGCTGT GGTAGTTGAG 
GGGAAACCAC TGCCAGACAT CATGTGGTAC AAGGATGAGG TGCTGCTGGC CGAGAGCAAC 
CATGTGAGCT TCGTGTATGA GGAGAACGAG TGTTCCCTAG TGCTGCTCAG CGCAGGAAGC 
CAGGATGGAG GCGTCTATAC CTGTACCGCC CGGAACCTGG CAGGCGAAGT CTCCTGCAAG 
GCGGAGCTGT CTGTGCTTTC AGCTCAGACA GCCATGGAGG TCGAGGGGGT CGGAGAGGAT 
GAGGAACACC GAGGAAGAAG ACTTAGCGAC TACTACGACA TCCACCAAGA GATTGGCAGG 
GGTGCCTTCT CCTACCTGCG GCGTGTAGTG GAGAGGAGCT CTGGCCTGGA GTTTGCAGCC 
AAGTTCATCC CTAGCCAGGC TAAGCCTAAA GCATCAGCAA GACGCGAGGC GCGCTTGCTG 
GCCCGGCTCC AGCATGGCTG TGTGCTCTAC TTCCACGAAG CCTTTGAGAG GCGCAGGGGA 
CTTGTCATAG TCACGGAGCT CTGCACAGAA GAGCTTCTAG AGCGCATGGC CAGGAAGCCC 
ACCGTGTGTG AGTCTGAGAC TCGGACCTAT ATGCGTCAAG TGCTAGAGGG GATATGCTAT 
CTGCATCAGA GCCATGTGCT GCACCTGGAT GTCAAGCCGG AGAACCTGCT TGTGTGGGAT 
GGTGCAGGGG GTGAAGAGCA GGTGCGCATC TGTGACTTCG GGAACGCCCA GGAACTCACT 
CCGGGAGAGC CCCAGTACTG TCAGTATGGC ACGCCAGAGT TTGTAGCACC TGAGATTGTC 
AACCAGAGCC CTGTGTCTGG AGTCACTGAC ATCTGGCCCG TGGGCGTCGT TGCCTTTCTC 
TGTTTGACGG GCATCTCCCC ATTTGTTGGG GAGAATGACC GGACAACACT CATGAATATC 
CGAAACTACA ACGTGGCATT TGAGGAGACT ACGTTCCTGA GTCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGCT GGTGCAGGAT CGACTGAGAC CTACTGCAGA AGAGACATTG 
GAACATCCTT GGTTCAAAAC AGAAGCAAAG GGTGCAGAGG TGAGCACAGA TCACCTGAAG 
CTCTTCCTGT CACGGAGGAG GTGGCAGCGC TCTCAAATCA GCTACAAGTG CCACCTGGTG 
CTGCGCCCCA TTCCGGAACT GCTACGGGCT CCCCCGGAGC GGGTGTGGGT GGCCATGCCT 
AGAAGACAAC CCCCGAGTGG GGGACTCTCA TCCTCTTCAG ACTCAGAAGA GGAAGAGTTG 
GAGGAACTTC CCTCAGTACC CCGCCCCCTG CAGCCTGAGT TCTCAGGTTC TCGGGTATCC 
CTCACCGACA TCCCCACCGA AGATGAGGCT CTGGGGACCC CGGAAGCTGG GGCTGCCACC 
CCCATGGACT GGCAAGAGCA GGAAAGGACT CCCTCTAAGG ACCAGGAGGC CCCCAGCCCT 
GAGGCACTCC CCTCTCCAGG CCAGGAGTCC CCTGATGGGC CTAGCCCCAG GCGGCCAGAG 
CTCCGCAGGG GTAGCTCTGC TGAGAGTGCC CTGCCCCGAG TCGGGTCGCG GGAACCGGGC 
CGGAGCTTGC ACAAGGCAGC ATCCGTGGAG CTGCCGCAGC GCAGGAGCCC CAGCCCGGGT 
GCCACCCGCC TGACTCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGTCAGC GGCTGCTGAG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGA 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCA CGGGATCCCA GGATGGCAAG GGCTGCCTCC 
AGCGAGGCTG CCCCCCACCA CCAGCCCCCT CCGGAGTCCC GGGGTCTGCA AAAGAGCAGC 
AGCTTCTCTC AGGGCGAGGC AGAGCCCCGG GGTCGGCACC GCCGCGCGGG GGCACCCCTC 
GAAATCCCCG TGGCCAGACT TGGGGCTCGC AGGCTGCAGG AGTCTCCCTC CTTATCTGCC 
CTTAGCGAGA CCCAGCCACC GAGCCCTGCG AGGCCCAGCG TCCCCAAACT CAGTATCACC 
AAGTCCCCGG AACCTTCCGC TGTCACATCC AGAGACTCTC CCCAGCCCCC GGAACCTCAG 
CCTGTCCCAG AAAAGGTCCC TGAGCCCAAA CCAGAACCAG TCCGAGCTGC AAAACCAGCA 
CAACCCCCTT TGGCTCTGCA AATGCCAACG CAGCCTCTCA CCCCCTATGC CCAGATCATG 
CAGTCGCTCC AGCTGTCCAG TCCCACCCTG TCCCCACAGG ACCCCGCTGT GCCTCCATCA 
GAGCCCAAGC CCCACGCGGC TGTGTTTGCC AGGGTGGCTT CCCCACCACC CGGAGTCTCT 
GAGAAGCGCG TGCCTTCGGC CAGAACTCCC CCAGTGCTAG CCGAGAAAGC CCGAGTCCCC 
ACGGTGCCCC CCAGGCCAGG CAGCAGCCTC AGCGGCAGCA TCGAAAACCT TGAATCAGAG 
GCTGTGTTCG AGGCTAAGTT CAAACGCAGC CGCGAATCGC CACTGTCGCG GGGCCTGAGG 
CTACTGAGCC GCTCGCGCTC GGAGGAGCGC GGTCCCTTCC GTGGGGCGGA GGACGACGGC 
ATATACCGGC CCAGTCCGGC AGGAACGCCG CTAGAGCTGG TGCGACGGCC CGAGCGCTCG 
CGCTCCGTGC AGGACCTCAG GGTAGCCGGA GAGCCGGGCC TTGTGCGTCG CCTTTCGCTG 
TCCCTGTCGC AGAAGCTGCG GCGGACACCG CCGGGACAGC GACACCCTGC CTGGGAGTCC 
CGCAGCGGAG ACGGGGAGAG CTCCGAGGGC GGCAGCTCAG CTCGGGCATC CCCAGTGCTG 
GCGGTACGCA GGAGACTCAG CTCCACGCTG GAGCGTCTGT CCAGCCGTTT GCAGCGCAGC 
GGCAGCAGCG AGGACTCCGG GGGCGCCTCG GGTCGCAGCA CGCCGCTGTT TGGACGGCTG 
CGCAGGGCTA CTTCGGAAGG GGAGAGCCTG CGACGCCTCG GTGTTCCGCA CAATCAGCTG 
GGCTCTCAGA CTGGTGCCAC TACACCTTCC GCTGAGTCTC TGGGCTCAGA GGCCAGTGGC 
ACATCGGGTT CTTCAGCTCC TGGAGAAAGC CGAAGCCGGC ACCGGTGGGG CCTTTCCAGG 
CTGCGCAAGG ACAAGGGATT ATCTCAGCCA AACCTCTCCT CCAGTGTCCA AGAGGACTTG 
GGTCACCAGT ATGTGCCCAG TGAGTCAGAT TTCCCCCCAG TCTTCCACAT CAAACTCAAG 
GACCAGGTGC TGTTGGAGGG GGAAGCAGCC ACCCTGCTCT GCCTACCCGC AGCCTGCCCA 
GCACCCCGCA TCTCCTGGAT GAAAGATAAG CAGTCCCTGC GTTCAGAGCC CTCAGTGGTC 
ATTGTGTCCT GCAAAGATGG ACGGCAGCTG CTGAGCATAC CCCGGGCCGG CAAGCGGCAT 
GCTGGGCTCT ATGAGTGCTC AGCCACCAAT GTCCTGGGCA GCATTACCAG CTCCTGTACT 
GTGGCCGTGG CCCGCATTCC AGGGAAGCTA GCTCCTCCAG AGGTGCCCCA GACCTACCAT 
GACACAGCAC TAGTAGTGTG GAAGCCAGGA GACGGTCGGG CACCTTGCAC ATACACTCTG 
GAGCGGCGGG TGGATGGGGA ATCTGTCTGG CACCCTGTGA GCTCAGGCAT CCCCGACTGT 
TATTACAATG TGACTCAGCT GCCTGTCGGC GTGACCGTGC GGTTCCGTGT GGCCTGTTCC 
AATCGTGCTG GGCAGGGGCC CTTCAGCAAC CCTTCTGAGA AGGTCTTCAT CCGAGGCACT 
CCAGATTCTC CTGCTCAGCC AGCTGCTGCT CCCCGAGATG CCCCTGTTAC CTCAGGGCCA 
ACCAGGGCCC CACCGCCTGA CTCTCCTACC TCACTGGCCC CCACCCCCGC TCTTGCGCCC 
CCAGCCTCCC AGGCATCCAC TCTCAGCCCC TCAACTTCCT CCATGTCTGC CAACCAGGCC 
TTGTCCTCAC TCAAGGCTGT GGGTCCACCA CCCGCAACCC CTCCACGAAA GCACAGGGGC 
CTTCTGGCCA CCCAGCAAGC AGAGCCATCC CCACCCAGTA TCGTGGTCAC CCCAAGTGAA 
CCCAGGTCTT TTGTCCCTGA CACTGGAACC CTGACCCCAA CCTCTAGTCC TCAAGGGGTT 
AAACCAGCAC CTTCCTCAAC TTCCTTGTAT ATGGTGACTT CCTTCGTGTC TGCACCTCCA 
GCCCCCCAGG CCCCAGCCCC TGAGCCCCCT CCTGAGCCCA CCAAGGTGAC TGTGCGGAGC 
CTCAGCCCAG CCAAGGAGGT GGTCAGCTCC CCAACTCCTG AGAGCACCAC TCTTCGGCAG 
GGTCCCCCTC AGAAACCCTA CACCTTCCTG GAGGAGAAGG CCAGGGGCCG CTTTGGCGTT 
GTGCGGTCAT GCCGGGAGAA TGCTACGGGC CGAACGTTTG TCGCCAAGAT CGTGCCGTAC 
GCCGCGGAGG GCAAGCGACG CGTGCTGCAG GAATACGAGG TGCTTCGGAC GCTGCACCAC 
GAGCGGCTCA TGTCCCTGCA CGAGGCCTAC ATCACCCCTC GGTACCTCGT GCTTATCGCT 
GAGAGCTGTG GCAACCGGGA ACTCCTCTGT GGGCTCAGCG ACAGATTCCG GTATTCAGAA 
GATGACGTGG CCACCTATGT GGTGCAGCTG CTACAAGGCC TGGACTACCT CCACGGCCAC 
CACGTGCTAC ACCTGGACAT CAAGCCGGAC AATCTACTGT TGGCCGCTGA CAATGCCCTT 
AAAATTGTGG ACTTCGGCAG TGCTCAGCCC TATAACCCTC AGGCCCTGAA GCCACTTGGC 
CACCGCACAG GCACTCTGGA GTTCATGGCT CCTGAGATGG TGAAGGGAGA CCCCATTGGC 
TCTGCCACGG ACATCTGGGG AGCGGGTGTG CTCACCTACA TCATGCTTAG TGGGTACTCC 
CCATTCTATG AGCCAGACCC CCAGGAAACA GAGGCTCGGA TTGTTGGGGG TCGCTTTGAT 
GCCTTCCAGT TGTATCCTAA CACATCCCAG AGTGCCACCC TCTTCTTGAG AAAGGTCCTC 
TCAGTACATC CCTGGAGCCG GCCCTCTCTG CAGGACTGCT TGGCCCACCC ATGGCTGCAA 
GATGCCTACC TGATGAAGCT GCGCCGCCAG ACACTCACCT TCACCACCAA CCGGCTCAAG 
GAATTCCTGG GCGAGCAGCG GCGACGTCGG GCTGAGGCTG CTACCCGTCA CAAGGTGCTG 
CTCCGCTCCT ACCCTGGCAG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_006496403.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496466.1 Mus musculus SPEG complex locus (Speg), transcript variant X15, mRNA.

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CloneID	OMu27706
Clone ID Related Accession (Same CDS sequence)	XM_006496404.4 , XM_006496404.3 , XM_006496403.3 , XM_006496403.4
Accession Version	XM_006496403.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496403.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCA GTCCCAGCCA GGATCATGAT TCCTCCGACT CTTCTTCCAA GGCACCCCCA 
ACGTTCAAGG TCTCACTCAT GGACCAATCG GTGAGAGAAG GTCAAGATGT CATTATGAGC 
ATCCGTGTGC AGGGAGAGCC CAAGCCTGTG GTTTCCTGGC TGAGGAATCG ACAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCAGAGGAG GCCGAGGGTG GGCTCTGCCG CTTGAGGATC 
CTGGCTGCTG AACGGGGCGA TGCTGGTTTC TACACATGCA AGGCGGTCAA CGAATATGGC 
GCTCGGCAGT GCGAGGCCCG CCTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCAG GTCCAGCTGA CGTCGAGGTG GACTGGCTGT GCCGGGGCCG CCTGCTGCAG 
CCTGCGCTGC TCAAATGCAA GATGCATTTT GATGGCCGCA AGTGCAAGCT GCTGCTCACC 
TCTGTGCACG AGGACGACAG TGGTGTCTAC ACCTGCAAGC TGAGCACAGC CAAAGATGAA 
CTGACCTGCA GTGCCCGGCT GACGGTTCGG CCGTCGCTGG CGCCTCTCTT CACCCGGTTG 
CTGGAAGATG TGGAGGTGTT GGAAGGCCGT GCTGCCCGCT TGGATTGCAA GATCAGCGGT 
ACCCCGCCTC CCTCCGTTAC CTGGACTCAT TTTGGCCACC CCGTGAATGA GGGTGACAAC 
TTGCGGCTTC AACAGGATGG AGGTCTACAC TCACTGCACA TTGCCCGGGT GGGCAGTGAG 
GACGAAGGAC TCTATGAAGT CAGTGCCACC AACACTCATG GCCAGGCCCA CTGCTCCGCC 
CAGCTGTATG TGGAGGAGCC TCGGACAGCT GCCTCTGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCTA TCCCTGAGGA GCCGGAGCAC GGAGATCTAG AGAGGCTGTC TATTCCTGAC 
TTCCTACGGC CACTGCAAGA TCTGGAAGTG GGACTAGCTA AGGAGGCCAT GCTGGAGTGC 
CAAGTGACCG GCCTGCCCTA TCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATTCAA 
AGCAGTGACG ACCGACGCAT GACACAGTAC AGGGACATAC ATCGCCTGGT GTTCCCAGCT 
GTGGGGCCTC AGCATGCTGG TGTCTATAAG AGTGTAATCG CCAACAAGCT GGGCAAAGCG 
GCCTGCTACG CCCATCTCTA TGTCACAGAT GTGGTTCCAG GCCCTCCAGA TGGTGCTCCG 
GAAGTAGTAG CTGTGACCGG AAGGATGGTC ACACTATCGT GGAACCCACC CAGGAGTCTG 
GACATGGCTA TTGACCCAGA TTCCCTAACA TACACTGTGC AGCACCAGGT GTTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCTG CGTGGGCAGC CACAGGGCTG 
AAGAAGGGGA TCCAGCACAT CTTCCGGGTC CTCAGCTCCA GTGGCAAGAG CAGCAGCAAG 
CCCTCAGCAC CGTCGGAACC CGTGCAGCTG CTAGAGCATG GCCCACCCCT GGAAGAGGCG 
CCTGCTGTGC TGGACAAGCA GGACATTGTG TATGTGGTAG AGGGCCAGCC TGCCTGTGTC 
ACCGTCACTT TTAACCATGT GGAGGCCCAG GTTGTCTGGA GGAGCTGCCG GGGAGCCCTC 
CTCGAGGCGC GCACAGGTGT GTATGAACTG AGTCAGCCAG ATGACGACCA GTATTGTCTG 
CGGATCTGCC GGGTGAGCCG ACGAGACCTG GGACCCCTTA CTTGCAGTGC CCGAAACCGC 
CACGGCACAA AGGCCTGCTC GGTCACCCTG GAGCTGGCAG AGGCACCTCG GTTTGAGTCT 
ATCATGGAAG ATGTGGAGGT GGGGCCTGGA GAAACCGCTC GCTTTGCTGT GGTAGTTGAG 
GGGAAACCAC TGCCAGACAT CATGTGGTAC AAGGATGAGG TGCTGCTGGC CGAGAGCAAC 
CATGTGAGCT TCGTGTATGA GGAGAACGAG TGTTCCCTAG TGCTGCTCAG CGCAGGAAGC 
CAGGATGGAG GCGTCTATAC CTGTACCGCC CGGAACCTGG CAGGCGAAGT CTCCTGCAAG 
GCGGAGCTGT CTGTGCTTTC AGCTCAGACA GCCATGGAGG TCGAGGGGGT CGGAGAGGAT 
GAGGAACACC GAGGAAGAAG ACTTAGCGAC TACTACGACA TCCACCAAGA GATTGGCAGG 
GGTGCCTTCT CCTACCTGCG GCGTGTAGTG GAGAGGAGCT CTGGCCTGGA GTTTGCAGCC 
AAGTTCATCC CTAGCCAGGC TAAGCCTAAA GCATCAGCAA GACGCGAGGC GCGCTTGCTG 
GCCCGGCTCC AGCATGGCTG TGTGCTCTAC TTCCACGAAG CCTTTGAGAG GCGCAGGGGA 
CTTGTCATAG TCACGGAGCT CTGCACAGAA GAGCTTCTAG AGCGCATGGC CAGGAAGCCC 
ACCGTGTGTG AGTCTGAGAC TCGGACCTAT ATGCGTCAAG TGCTAGAGGG GATATGCTAT 
CTGCATCAGA GCCATGTGCT GCACCTGGAT GTCAAGCCGG AGAACCTGCT TGTGTGGGAT 
GGTGCAGGGG GTGAAGAGCA GGTGCGCATC TGTGACTTCG GGAACGCCCA GGAACTCACT 
CCGGGAGAGC CCCAGTACTG TCAGTATGGC ACGCCAGAGT TTGTAGCACC TGAGATTGTC 
AACCAGAGCC CTGTGTCTGG AGTCACTGAC ATCTGGCCCG TGGGCGTCGT TGCCTTTCTC 
TGTTTGACGG GCATCTCCCC ATTTGTTGGG GAGAATGACC GGACAACACT CATGAATATC 
CGAAACTACA ACGTGGCATT TGAGGAGACT ACGTTCCTGA GTCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGCT GGTGCAGGAT CGACTGAGAC CTACTGCAGA AGAGACATTG 
GAACATCCTT GGTTCAAAAC AGAAGCAAAG GGTGCAGAGG TGAGCACAGA TCACCTGAAG 
CTCTTCCTGT CACGGAGGAG GTGGCAGCGC TCTCAAATCA GCTACAAGTG CCACCTGGTG 
CTGCGCCCCA TTCCGGAACT GCTACGGGCT CCCCCGGAGC GGGTGTGGGT GGCCATGCCT 
AGAAGACAAC CCCCGAGTGG GGGACTCTCA TCCTCTTCAG ACTCAGAAGA GGAAGAGTTG 
GAGGAACTTC CCTCAGTACC CCGCCCCCTG CAGCCTGAGT TCTCAGGTTC TCGGGTATCC 
CTCACCGACA TCCCCACCGA AGATGAGGCT CTGGGGACCC CGGAAGCTGG GGCTGCCACC 
CCCATGGACT GGCAAGAGCA GGAAAGGACT CCCTCTAAGG ACCAGGAGGC CCCCAGCCCT 
GAGGCACTCC CCTCTCCAGG CCAGGAGTCC CCTGATGGGC CTAGCCCCAG GCGGCCAGAG 
CTCCGCAGGG GTAGCTCTGC TGAGAGTGCC CTGCCCCGAG TCGGGTCGCG GGAACCGGGC 
CGGAGCTTGC ACAAGGCAGC ATCCGTGGAG CTGCCGCAGC GCAGGAGCCC CAGCCCGGGT 
GCCACCCGCC TGACTCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGTCAGC GGCTGCTGAG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGA 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCA CGGGATCCCA GGATGGCAAG GGCTGCCTCC 
AGCGAGGCTG CCCCCCACCA CCAGCCCCCT CCGGAGTCCC GGGGTCTGCA AAAGAGCAGC 
AGCTTCTCTC AGGGCGAGGC AGAGCCCCGG GGTCGGCACC GCCGCGCGGG GGCACCCCTC 
GAAATCCCCG TGGCCAGACT TGGGGCTCGC AGGCTGCAGG AGTCTCCCTC CTTATCTGCC 
CTTAGCGAGA CCCAGCCACC GAGCCCTGCG AGGCCCAGCG TCCCCAAACT CAGTATCACC 
AAGTCCCCGG AACCTTCCGC TGTCACATCC AGAGACTCTC CCCAGCCCCC GGAACCTCAG 
CCTGTCCCAG AAAAGGTCCC TGAGCCCAAA CCAGAACCAG TCCGAGCTGC AAAACCAGCA 
CAACCCCCTT TGGCTCTGCA AATGCCAACG CAGCCTCTCA CCCCCTATGC CCAGATCATG 
CAGTCGCTCC AGCTGTCCAG TCCCACCCTG TCCCCACAGG ACCCCGCTGT GCCTCCATCA 
GAGCCCAAGC CCCACGCGGC TGTGTTTGCC AGGGTGGCTT CCCCACCACC CGGAGTCTCT 
GAGAAGCGCG TGCCTTCGGC CAGAACTCCC CCAGTGCTAG CCGAGAAAGC CCGAGTCCCC 
ACGGTGCCCC CCAGGCCAGG CAGCAGCCTC AGCGGCAGCA TCGAAAACCT TGAATCAGAG 
GCTGTGTTCG AGGCTAAGTT CAAACGCAGC CGCGAATCGC CACTGTCGCG GGGCCTGAGG 
CTACTGAGCC GCTCGCGCTC GGAGGAGCGC GGTCCCTTCC GTGGGGCGGA GGACGACGGC 
ATATACCGGC CCAGTCCGGC AGGAACGCCG CTAGAGCTGG TGCGACGGCC CGAGCGCTCG 
CGCTCCGTGC AGGACCTCAG GGTAGCCGGA GAGCCGGGCC TTGTGCGTCG CCTTTCGCTG 
TCCCTGTCGC AGAAGCTGCG GCGGACACCG CCGGGACAGC GACACCCTGC CTGGGAGTCC 
CGCAGCGGAG ACGGGGAGAG CTCCGAGGGC GGCAGCTCAG CTCGGGCATC CCCAGTGCTG 
GCGGTACGCA GGAGACTCAG CTCCACGCTG GAGCGTCTGT CCAGCCGTTT GCAGCGCAGC 
GGCAGCAGCG AGGACTCCGG GGGCGCCTCG GGTCGCAGCA CGCCGCTGTT TGGACGGCTG 
CGCAGGGCTA CTTCGGAAGG GGAGAGCCTG CGACGCCTCG GTGTTCCGCA CAATCAGCTG 
GGCTCTCAGA CTGGTGCCAC TACACCTTCC GCTGAGTCTC TGGGCTCAGA GGCCAGTGGC 
ACATCGGGTT CTTCAGCTCC TGGAGAAAGC CGAAGCCGGC ACCGGTGGGG CCTTTCCAGG 
CTGCGCAAGG ACAAGGGATT ATCTCAGCCA AACCTCTCCT CCAGTGTCCA AGAGGACTTG 
GGTCACCAGT ATGTGCCCAG TGAGTCAGAT TTCCCCCCAG TCTTCCACAT CAAACTCAAG 
GACCAGGTGC TGTTGGAGGG GGAAGCAGCC ACCCTGCTCT GCCTACCCGC AGCCTGCCCA 
GCACCCCGCA TCTCCTGGAT GAAAGATAAG CAGTCCCTGC GTTCAGAGCC CTCAGTGGTC 
ATTGTGTCCT GCAAAGATGG ACGGCAGCTG CTGAGCATAC CCCGGGCCGG CAAGCGGCAT 
GCTGGGCTCT ATGAGTGCTC AGCCACCAAT GTCCTGGGCA GCATTACCAG CTCCTGTACT 
GTGGCCGTGG CCCGCATTCC AGGGAAGCTA GCTCCTCCAG AGGTGCCCCA GACCTACCAT 
GACACAGCAC TAGTAGTGTG GAAGCCAGGA GACGGTCGGG CACCTTGCAC ATACACTCTG 
GAGCGGCGGG TGGATGGGGA ATCTGTCTGG CACCCTGTGA GCTCAGGCAT CCCCGACTGT 
TATTACAATG TGACTCAGCT GCCTGTCGGC GTGACCGTGC GGTTCCGTGT GGCCTGTTCC 
AATCGTGCTG GGCAGGGGCC CTTCAGCAAC CCTTCTGAGA AGGTCTTCAT CCGAGGCACT 
CCAGATTCTC CTGCTCAGCC AGCTGCTGCT CCCCGAGATG CCCCTGTTAC CTCAGGGCCA 
ACCAGGGCCC CACCGCCTGA CTCTCCTACC TCACTGGCCC CCACCCCCGC TCTTGCGCCC 
CCAGCCTCCC AGGCATCCAC TCTCAGCCCC TCAACTTCCT CCATGTCTGC CAACCAGGCC 
TTGTCCTCAC TCAAGGCTGT GGGTCCACCA CCCGCAACCC CTCCACGAAA GCACAGGGGC 
CTTCTGGCCA CCCAGCAAGC AGAGCCATCC CCACCCAGTA TCGTGGTCAC CCCAAGTGAA 
CCCAGGTCTT TTGTCCCTGA CACTGGAACC CTGACCCCAA CCTCTAGTCC TCAAGGGGTT 
AAACCAGCAC CTTCCTCAAC TTCCTTGTAT ATGGTGACTT CCTTCGTGTC TGCACCTCCA 
GCCCCCCAGG CCCCAGCCCC TGAGCCCCCT CCTGAGCCCA CCAAGGTGAC TGTGCGGAGC 
CTCAGCCCAG CCAAGGAGGT GGTCAGCTCC CCAACTCCTG AGAGCACCAC TCTTCGGCAG 
GGTCCCCCTC AGAAACCCTA CACCTTCCTG GAGGAGAAGG CCAGGGGCCG CTTTGGCGTT 
GTGCGGTCAT GCCGGGAGAA TGCTACGGGC CGAACGTTTG TCGCCAAGAT CGTGCCGTAC 
GCCGCGGAGG GCAAGCGACG CGTGCTGCAG GAATACGAGG TGCTTCGGAC GCTGCACCAC 
GAGCGGCTCA TGTCCCTGCA CGAGGCCTAC ATCACCCCTC GGTACCTCGT GCTTATCGCT 
GAGAGCTGTG GCAACCGGGA ACTCCTCTGT GGGCTCAGCG ACAGATTCCG GTATTCAGAA 
GATGACGTGG CCACCTATGT GGTGCAGCTG CTACAAGGCC TGGACTACCT CCACGGCCAC 
CACGTGCTAC ACCTGGACAT CAAGCCGGAC AATCTACTGT TGGCCGCTGA CAATGCCCTT 
AAAATTGTGG ACTTCGGCAG TGCTCAGCCC TATAACCCTC AGGCCCTGAA GCCACTTGGC 
CACCGCACAG GCACTCTGGA GTTCATGGCT CCTGAGATGG TGAAGGGAGA CCCCATTGGC 
TCTGCCACGG ACATCTGGGG AGCGGGTGTG CTCACCTACA TCATGCTTAG TGGGTACTCC 
CCATTCTATG AGCCAGACCC CCAGGAAACA GAGGCTCGGA TTGTTGGGGG TCGCTTTGAT 
GCCTTCCAGT TGTATCCTAA CACATCCCAG AGTGCCACCC TCTTCTTGAG AAAGGTCCTC 
TCAGTACATC CCTGGAGCCG GCCCTCTCTG CAGGACTGCT TGGCCCACCC ATGGCTGCAA 
GATGCCTACC TGATGAAGCT GCGCCGCCAG ACACTCACCT TCACCACCAA CCGGCTCAAG 
GAATTCCTGG GCGAGCAGCG GCGACGTCGG GCTGAGGCTG CTACCCGTCA CAAGGTGCTG 
CTCCGCTCCT ACCCTGGCAG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496466.1
CDS	896..8122
Translation	MKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_006496403.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496403.4

ORF Insert Sequence:

ATGAAGCTCA GTCCCAGCCA GGATCATGAT TCCTCCGACT CTTCTTCCAA GGCACCCCCA 
ACGTTCAAGG TCTCACTCAT GGACCAATCG GTGAGAGAAG GTCAAGATGT CATTATGAGC 
ATCCGTGTGC AGGGAGAGCC CAAGCCTGTG GTTTCCTGGC TGAGGAATCG ACAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCAGAGGAG GCCGAGGGTG GGCTCTGCCG CTTGAGGATC 
CTGGCTGCTG AACGGGGCGA TGCTGGTTTC TACACATGCA AGGCGGTCAA CGAATATGGC 
GCTCGGCAGT GCGAGGCCCG CCTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCAG GTCCAGCTGA CGTCGAGGTG GACTGGCTGT GCCGGGGCCG CCTGCTGCAG 
CCTGCGCTGC TCAAATGCAA GATGCATTTT GATGGCCGCA AGTGCAAGCT GCTGCTCACC 
TCTGTGCACG AGGACGACAG TGGTGTCTAC ACCTGCAAGC TGAGCACAGC CAAAGATGAA 
CTGACCTGCA GTGCCCGGCT GACGGTTCGG CCGTCGCTGG CGCCTCTCTT CACCCGGTTG 
CTGGAAGATG TGGAGGTGTT GGAAGGCCGT GCTGCCCGCT TGGATTGCAA GATCAGCGGT 
ACCCCGCCTC CCTCCGTTAC CTGGACTCAT TTTGGCCACC CCGTGAATGA GGGTGACAAC 
TTGCGGCTTC AACAGGATGG AGGTCTACAC TCACTGCACA TTGCCCGGGT GGGCAGTGAG 
GACGAAGGAC TCTATGAAGT CAGTGCCACC AACACTCATG GCCAGGCCCA CTGCTCCGCC 
CAGCTGTATG TGGAGGAGCC TCGGACAGCT GCCTCTGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCTA TCCCTGAGGA GCCGGAGCAC GGAGATCTAG AGAGGCTGTC TATTCCTGAC 
TTCCTACGGC CACTGCAAGA TCTGGAAGTG GGACTAGCTA AGGAGGCCAT GCTGGAGTGC 
CAAGTGACCG GCCTGCCCTA TCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATTCAA 
AGCAGTGACG ACCGACGCAT GACACAGTAC AGGGACATAC ATCGCCTGGT GTTCCCAGCT 
GTGGGGCCTC AGCATGCTGG TGTCTATAAG AGTGTAATCG CCAACAAGCT GGGCAAAGCG 
GCCTGCTACG CCCATCTCTA TGTCACAGAT GTGGTTCCAG GCCCTCCAGA TGGTGCTCCG 
GAAGTAGTAG CTGTGACCGG AAGGATGGTC ACACTATCGT GGAACCCACC CAGGAGTCTG 
GACATGGCTA TTGACCCAGA TTCCCTAACA TACACTGTGC AGCACCAGGT GTTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCTG CGTGGGCAGC CACAGGGCTG 
AAGAAGGGGA TCCAGCACAT CTTCCGGGTC CTCAGCTCCA GTGGCAAGAG CAGCAGCAAG 
CCCTCAGCAC CGTCGGAACC CGTGCAGCTG CTAGAGCATG GCCCACCCCT GGAAGAGGCG 
CCTGCTGTGC TGGACAAGCA GGACATTGTG TATGTGGTAG AGGGCCAGCC TGCCTGTGTC 
ACCGTCACTT TTAACCATGT GGAGGCCCAG GTTGTCTGGA GGAGCTGCCG GGGAGCCCTC 
CTCGAGGCGC GCACAGGTGT GTATGAACTG AGTCAGCCAG ATGACGACCA GTATTGTCTG 
CGGATCTGCC GGGTGAGCCG ACGAGACCTG GGACCCCTTA CTTGCAGTGC CCGAAACCGC 
CACGGCACAA AGGCCTGCTC GGTCACCCTG GAGCTGGCAG AGGCACCTCG GTTTGAGTCT 
ATCATGGAAG ATGTGGAGGT GGGGCCTGGA GAAACCGCTC GCTTTGCTGT GGTAGTTGAG 
GGGAAACCAC TGCCAGACAT CATGTGGTAC AAGGATGAGG TGCTGCTGGC CGAGAGCAAC 
CATGTGAGCT TCGTGTATGA GGAGAACGAG TGTTCCCTAG TGCTGCTCAG CGCAGGAAGC 
CAGGATGGAG GCGTCTATAC CTGTACCGCC CGGAACCTGG CAGGCGAAGT CTCCTGCAAG 
GCGGAGCTGT CTGTGCTTTC AGCTCAGACA GCCATGGAGG TCGAGGGGGT CGGAGAGGAT 
GAGGAACACC GAGGAAGAAG ACTTAGCGAC TACTACGACA TCCACCAAGA GATTGGCAGG 
GGTGCCTTCT CCTACCTGCG GCGTGTAGTG GAGAGGAGCT CTGGCCTGGA GTTTGCAGCC 
AAGTTCATCC CTAGCCAGGC TAAGCCTAAA GCATCAGCAA GACGCGAGGC GCGCTTGCTG 
GCCCGGCTCC AGCATGGCTG TGTGCTCTAC TTCCACGAAG CCTTTGAGAG GCGCAGGGGA 
CTTGTCATAG TCACGGAGCT CTGCACAGAA GAGCTTCTAG AGCGCATGGC CAGGAAGCCC 
ACCGTGTGTG AGTCTGAGAC TCGGACCTAT ATGCGTCAAG TGCTAGAGGG GATATGCTAT 
CTGCATCAGA GCCATGTGCT GCACCTGGAT GTCAAGCCGG AGAACCTGCT TGTGTGGGAT 
GGTGCAGGGG GTGAAGAGCA GGTGCGCATC TGTGACTTCG GGAACGCCCA GGAACTCACT 
CCGGGAGAGC CCCAGTACTG TCAGTATGGC ACGCCAGAGT TTGTAGCACC TGAGATTGTC 
AACCAGAGCC CTGTGTCTGG AGTCACTGAC ATCTGGCCCG TGGGCGTCGT TGCCTTTCTC 
TGTTTGACGG GCATCTCCCC ATTTGTTGGG GAGAATGACC GGACAACACT CATGAATATC 
CGAAACTACA ACGTGGCATT TGAGGAGACT ACGTTCCTGA GTCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGCT GGTGCAGGAT CGACTGAGAC CTACTGCAGA AGAGACATTG 
GAACATCCTT GGTTCAAAAC AGAAGCAAAG GGTGCAGAGG TGAGCACAGA TCACCTGAAG 
CTCTTCCTGT CACGGAGGAG GTGGCAGCGC TCTCAAATCA GCTACAAGTG CCACCTGGTG 
CTGCGCCCCA TTCCGGAACT GCTACGGGCT CCCCCGGAGC GGGTGTGGGT GGCCATGCCT 
AGAAGACAAC CCCCGAGTGG GGGACTCTCA TCCTCTTCAG ACTCAGAAGA GGAAGAGTTG 
GAGGAACTTC CCTCAGTACC CCGCCCCCTG CAGCCTGAGT TCTCAGGTTC TCGGGTATCC 
CTCACCGACA TCCCCACCGA AGATGAGGCT CTGGGGACCC CGGAAGCTGG GGCTGCCACC 
CCCATGGACT GGCAAGAGCA GGAAAGGACT CCCTCTAAGG ACCAGGAGGC CCCCAGCCCT 
GAGGCACTCC CCTCTCCAGG CCAGGAGTCC CCTGATGGGC CTAGCCCCAG GCGGCCAGAG 
CTCCGCAGGG GTAGCTCTGC TGAGAGTGCC CTGCCCCGAG TCGGGTCGCG GGAACCGGGC 
CGGAGCTTGC ACAAGGCAGC ATCCGTGGAG CTGCCGCAGC GCAGGAGCCC CAGCCCGGGT 
GCCACCCGCC TGACTCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGTCAGC GGCTGCTGAG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGA 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCA CGGGATCCCA GGATGGCAAG GGCTGCCTCC 
AGCGAGGCTG CCCCCCACCA CCAGCCCCCT CCGGAGTCCC GGGGTCTGCA AAAGAGCAGC 
AGCTTCTCTC AGGGCGAGGC AGAGCCCCGG GGTCGGCACC GCCGCGCGGG GGCACCCCTC 
GAAATCCCCG TGGCCAGACT TGGGGCTCGC AGGCTGCAGG AGTCTCCCTC CTTATCTGCC 
CTTAGCGAGA CCCAGCCACC GAGCCCTGCG AGGCCCAGCG TCCCCAAACT CAGTATCACC 
AAGTCCCCGG AACCTTCCGC TGTCACATCC AGAGACTCTC CCCAGCCCCC GGAACCTCAG 
CCTGTCCCAG AAAAGGTCCC TGAGCCCAAA CCAGAACCAG TCCGAGCTGC AAAACCAGCA 
CAACCCCCTT TGGCTCTGCA AATGCCAACG CAGCCTCTCA CCCCCTATGC CCAGATCATG 
CAGTCGCTCC AGCTGTCCAG TCCCACCCTG TCCCCACAGG ACCCCGCTGT GCCTCCATCA 
GAGCCCAAGC CCCACGCGGC TGTGTTTGCC AGGGTGGCTT CCCCACCACC CGGAGTCTCT 
GAGAAGCGCG TGCCTTCGGC CAGAACTCCC CCAGTGCTAG CCGAGAAAGC CCGAGTCCCC 
ACGGTGCCCC CCAGGCCAGG CAGCAGCCTC AGCGGCAGCA TCGAAAACCT TGAATCAGAG 
GCTGTGTTCG AGGCTAAGTT CAAACGCAGC CGCGAATCGC CACTGTCGCG GGGCCTGAGG 
CTACTGAGCC GCTCGCGCTC GGAGGAGCGC GGTCCCTTCC GTGGGGCGGA GGACGACGGC 
ATATACCGGC CCAGTCCGGC AGGAACGCCG CTAGAGCTGG TGCGACGGCC CGAGCGCTCG 
CGCTCCGTGC AGGACCTCAG GGTAGCCGGA GAGCCGGGCC TTGTGCGTCG CCTTTCGCTG 
TCCCTGTCGC AGAAGCTGCG GCGGACACCG CCGGGACAGC GACACCCTGC CTGGGAGTCC 
CGCAGCGGAG ACGGGGAGAG CTCCGAGGGC GGCAGCTCAG CTCGGGCATC CCCAGTGCTG 
GCGGTACGCA GGAGACTCAG CTCCACGCTG GAGCGTCTGT CCAGCCGTTT GCAGCGCAGC 
GGCAGCAGCG AGGACTCCGG GGGCGCCTCG GGTCGCAGCA CGCCGCTGTT TGGACGGCTG 
CGCAGGGCTA CTTCGGAAGG GGAGAGCCTG CGACGCCTCG GTGTTCCGCA CAATCAGCTG 
GGCTCTCAGA CTGGTGCCAC TACACCTTCC GCTGAGTCTC TGGGCTCAGA GGCCAGTGGC 
ACATCGGGTT CTTCAGCTCC TGGAGAAAGC CGAAGCCGGC ACCGGTGGGG CCTTTCCAGG 
CTGCGCAAGG ACAAGGGATT ATCTCAGCCA AACCTCTCCT CCAGTGTCCA AGAGGACTTG 
GGTCACCAGT ATGTGCCCAG TGAGTCAGAT TTCCCCCCAG TCTTCCACAT CAAACTCAAG 
GACCAGGTGC TGTTGGAGGG GGAAGCAGCC ACCCTGCTCT GCCTACCCGC AGCCTGCCCA 
GCACCCCGCA TCTCCTGGAT GAAAGATAAG CAGTCCCTGC GTTCAGAGCC CTCAGTGGTC 
ATTGTGTCCT GCAAAGATGG ACGGCAGCTG CTGAGCATAC CCCGGGCCGG CAAGCGGCAT 
GCTGGGCTCT ATGAGTGCTC AGCCACCAAT GTCCTGGGCA GCATTACCAG CTCCTGTACT 
GTGGCCGTGG CCCGCATTCC AGGGAAGCTA GCTCCTCCAG AGGTGCCCCA GACCTACCAT 
GACACAGCAC TAGTAGTGTG GAAGCCAGGA GACGGTCGGG CACCTTGCAC ATACACTCTG 
GAGCGGCGGG TGGATGGGGA ATCTGTCTGG CACCCTGTGA GCTCAGGCAT CCCCGACTGT 
TATTACAATG TGACTCAGCT GCCTGTCGGC GTGACCGTGC GGTTCCGTGT GGCCTGTTCC 
AATCGTGCTG GGCAGGGGCC CTTCAGCAAC CCTTCTGAGA AGGTCTTCAT CCGAGGCACT 
CCAGATTCTC CTGCTCAGCC AGCTGCTGCT CCCCGAGATG CCCCTGTTAC CTCAGGGCCA 
ACCAGGGCCC CACCGCCTGA CTCTCCTACC TCACTGGCCC CCACCCCCGC TCTTGCGCCC 
CCAGCCTCCC AGGCATCCAC TCTCAGCCCC TCAACTTCCT CCATGTCTGC CAACCAGGCC 
TTGTCCTCAC TCAAGGCTGT GGGTCCACCA CCCGCAACCC CTCCACGAAA GCACAGGGGC 
CTTCTGGCCA CCCAGCAAGC AGAGCCATCC CCACCCAGTA TCGTGGTCAC CCCAAGTGAA 
CCCAGGTCTT TTGTCCCTGA CACTGGAACC CTGACCCCAA CCTCTAGTCC TCAAGGGGTT 
AAACCAGCAC CTTCCTCAAC TTCCTTGTAT ATGGTGACTT CCTTCGTGTC TGCACCTCCA 
GCCCCCCAGG CCCCAGCCCC TGAGCCCCCT CCTGAGCCCA CCAAGGTGAC TGTGCGGAGC 
CTCAGCCCAG CCAAGGAGGT GGTCAGCTCC CCAACTCCTG AGAGCACCAC TCTTCGGCAG 
GGTCCCCCTC AGAAACCCTA CACCTTCCTG GAGGAGAAGG CCAGGGGCCG CTTTGGCGTT 
GTGCGGTCAT GCCGGGAGAA TGCTACGGGC CGAACGTTTG TCGCCAAGAT CGTGCCGTAC 
GCCGCGGAGG GCAAGCGACG CGTGCTGCAG GAATACGAGG TGCTTCGGAC GCTGCACCAC 
GAGCGGCTCA TGTCCCTGCA CGAGGCCTAC ATCACCCCTC GGTACCTCGT GCTTATCGCT 
GAGAGCTGTG GCAACCGGGA ACTCCTCTGT GGGCTCAGCG ACAGATTCCG GTATTCAGAA 
GATGACGTGG CCACCTATGT GGTGCAGCTG CTACAAGGCC TGGACTACCT CCACGGCCAC 
CACGTGCTAC ACCTGGACAT CAAGCCGGAC AATCTACTGT TGGCCGCTGA CAATGCCCTT 
AAAATTGTGG ACTTCGGCAG TGCTCAGCCC TATAACCCTC AGGCCCTGAA GCCACTTGGC 
CACCGCACAG GCACTCTGGA GTTCATGGCT CCTGAGATGG TGAAGGGAGA CCCCATTGGC 
TCTGCCACGG ACATCTGGGG AGCGGGTGTG CTCACCTACA TCATGCTTAG TGGGTACTCC 
CCATTCTATG AGCCAGACCC CCAGGAAACA GAGGCTCGGA TTGTTGGGGG TCGCTTTGAT 
GCCTTCCAGT TGTATCCTAA CACATCCCAG AGTGCCACCC TCTTCTTGAG AAAGGTCCTC 
TCAGTACATC CCTGGAGCCG GCCCTCTCTG CAGGACTGCT TGGCCCACCC ATGGCTGCAA 
GATGCCTACC TGATGAAGCT GCGCCGCCAG ACACTCACCT TCACCACCAA CCGGCTCAAG 
GAATTCCTGG GCGAGCAGCG GCGACGTCGG GCTGAGGCTG CTACCCGTCA CAAGGTGCTG 
CTCCGCTCCT ACCCTGGCAG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( NM_001085370.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001078839.1 Mus musculus SPEG complex locus (Speg), transcript variant 2, mRNA.

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CloneID	OMu20941
Clone ID Related Accession (Same CDS sequence)	XM_006496400.2 , XM_011238648.2 , XM_030243284.1 , XM_006496401.2 , XM_011238649.2 , XM_011238649.3 , NM_001085370.1 , XM_011238648.3 , XM_030243273.1
Accession Version	NM_001085370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147475.1, AC114651.12 and AK122488.1. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1. These differences produce a unique 5' UTR and cause translation initiation at a downstream start codon, compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1. This variant is similar, but not identical, to variant SPEGalpha described in PubMed ID: 10973969. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147475.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078839.1
CDS	1449..9032
Translation	MVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	NM_001085370.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001085370.1

ORF Insert Sequence:

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_006496400.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496463.1 Mus musculus SPEG complex locus (Speg), transcript variant X12, mRNA.

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CloneID	OMu20941
Clone ID Related Accession (Same CDS sequence)	XM_006496400.2 , XM_011238648.2 , XM_030243284.1 , XM_006496401.2 , XM_011238649.2 , XM_011238649.3 , NM_001085370.1 , XM_011238648.3 , XM_030243273.1
Accession Version	XM_006496400.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006496400.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496463.1
CDS	275..7858
Translation	MVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_006496400.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496400.2

ORF Insert Sequence:

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_011238648.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236950.1 Mus musculus SPEG complex locus (Speg), transcript variant X8, mRNA.

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CloneID	OMu20941
Clone ID Related Accession (Same CDS sequence)	XM_006496400.2 , XM_011238648.2 , XM_030243284.1 , XM_006496401.2 , XM_011238649.2 , XM_011238649.3 , NM_001085370.1 , XM_011238648.3 , XM_030243273.1
Accession Version	XM_011238648.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238648.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236950.1
CDS	2304..9887
Translation	MVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_011238648.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238648.2

ORF Insert Sequence:

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_006496401.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496464.1 Mus musculus SPEG complex locus (Speg), transcript variant X13, mRNA.

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CloneID	OMu20941
Clone ID Related Accession (Same CDS sequence)	XM_006496400.2 , XM_011238648.2 , XM_030243284.1 , XM_006496401.2 , XM_011238649.2 , XM_011238649.3 , NM_001085370.1 , XM_011238648.3 , XM_030243273.1
Accession Version	XM_006496401.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006496401.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496464.1
CDS	195..7778
Translation	MVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_006496401.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496401.2

ORF Insert Sequence:

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_011238649.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236951.1 Mus musculus SPEG complex locus (Speg), transcript variant X9, mRNA.

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CloneID	OMu20941
Clone ID Related Accession (Same CDS sequence)	XM_006496400.2 , XM_011238648.2 , XM_030243284.1 , XM_006496401.2 , XM_011238649.2 , XM_011238649.3 , NM_001085370.1 , XM_011238648.3 , XM_030243273.1
Accession Version	XM_011238649.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236951.1
CDS	1740..9323
Translation	MVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_011238649.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238649.2

ORF Insert Sequence:

ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_006496402.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496465.1 Mus musculus SPEG complex locus (Speg), transcript variant X14, mRNA.

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CloneID	OMu27705
Clone ID Related Accession (Same CDS sequence)	XM_006496402.2
Accession Version	XM_006496402.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496402.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATC TAACTCTGCT TTTCCATTGC CTGAGAATCT GGGGTTCATG CTGTAGGGTG 
GGGCCAACAG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496465.1
CDS	30..7433
Translation	MDDLTLLFHCLRIWGSCCRVGPTGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_006496402.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496402.2

ORF Insert Sequence:

ATGGATGATC TAACTCTGCT TTTCCATTGC CTGAGAATCT GGGGTTCATG CTGTAGGGTG 
GGGCCAACAG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_006496404.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496467.1 Mus musculus SPEG complex locus (Speg), transcript variant X14, mRNA.

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CloneID	OMu27706
Clone ID Related Accession (Same CDS sequence)	XM_006496404.4 , XM_006496404.3 , XM_006496403.3 , XM_006496403.4
Accession Version	XM_006496404.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496404.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCA GTCCCAGCCA GGATCATGAT TCCTCCGACT CTTCTTCCAA GGCACCCCCA 
ACGTTCAAGG TCTCACTCAT GGACCAATCG GTGAGAGAAG GTCAAGATGT CATTATGAGC 
ATCCGTGTGC AGGGAGAGCC CAAGCCTGTG GTTTCCTGGC TGAGGAATCG ACAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCAGAGGAG GCCGAGGGTG GGCTCTGCCG CTTGAGGATC 
CTGGCTGCTG AACGGGGCGA TGCTGGTTTC TACACATGCA AGGCGGTCAA CGAATATGGC 
GCTCGGCAGT GCGAGGCCCG CCTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCAG GTCCAGCTGA CGTCGAGGTG GACTGGCTGT GCCGGGGCCG CCTGCTGCAG 
CCTGCGCTGC TCAAATGCAA GATGCATTTT GATGGCCGCA AGTGCAAGCT GCTGCTCACC 
TCTGTGCACG AGGACGACAG TGGTGTCTAC ACCTGCAAGC TGAGCACAGC CAAAGATGAA 
CTGACCTGCA GTGCCCGGCT GACGGTTCGG CCGTCGCTGG CGCCTCTCTT CACCCGGTTG 
CTGGAAGATG TGGAGGTGTT GGAAGGCCGT GCTGCCCGCT TGGATTGCAA GATCAGCGGT 
ACCCCGCCTC CCTCCGTTAC CTGGACTCAT TTTGGCCACC CCGTGAATGA GGGTGACAAC 
TTGCGGCTTC AACAGGATGG AGGTCTACAC TCACTGCACA TTGCCCGGGT GGGCAGTGAG 
GACGAAGGAC TCTATGAAGT CAGTGCCACC AACACTCATG GCCAGGCCCA CTGCTCCGCC 
CAGCTGTATG TGGAGGAGCC TCGGACAGCT GCCTCTGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCTA TCCCTGAGGA GCCGGAGCAC GGAGATCTAG AGAGGCTGTC TATTCCTGAC 
TTCCTACGGC CACTGCAAGA TCTGGAAGTG GGACTAGCTA AGGAGGCCAT GCTGGAGTGC 
CAAGTGACCG GCCTGCCCTA TCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATTCAA 
AGCAGTGACG ACCGACGCAT GACACAGTAC AGGGACATAC ATCGCCTGGT GTTCCCAGCT 
GTGGGGCCTC AGCATGCTGG TGTCTATAAG AGTGTAATCG CCAACAAGCT GGGCAAAGCG 
GCCTGCTACG CCCATCTCTA TGTCACAGAT GTGGTTCCAG GCCCTCCAGA TGGTGCTCCG 
GAAGTAGTAG CTGTGACCGG AAGGATGGTC ACACTATCGT GGAACCCACC CAGGAGTCTG 
GACATGGCTA TTGACCCAGA TTCCCTAACA TACACTGTGC AGCACCAGGT GTTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCTG CGTGGGCAGC CACAGGGCTG 
AAGAAGGGGA TCCAGCACAT CTTCCGGGTC CTCAGCTCCA GTGGCAAGAG CAGCAGCAAG 
CCCTCAGCAC CGTCGGAACC CGTGCAGCTG CTAGAGCATG GCCCACCCCT GGAAGAGGCG 
CCTGCTGTGC TGGACAAGCA GGACATTGTG TATGTGGTAG AGGGCCAGCC TGCCTGTGTC 
ACCGTCACTT TTAACCATGT GGAGGCCCAG GTTGTCTGGA GGAGCTGCCG GGGAGCCCTC 
CTCGAGGCGC GCACAGGTGT GTATGAACTG AGTCAGCCAG ATGACGACCA GTATTGTCTG 
CGGATCTGCC GGGTGAGCCG ACGAGACCTG GGACCCCTTA CTTGCAGTGC CCGAAACCGC 
CACGGCACAA AGGCCTGCTC GGTCACCCTG GAGCTGGCAG AGGCACCTCG GTTTGAGTCT 
ATCATGGAAG ATGTGGAGGT GGGGCCTGGA GAAACCGCTC GCTTTGCTGT GGTAGTTGAG 
GGGAAACCAC TGCCAGACAT CATGTGGTAC AAGGATGAGG TGCTGCTGGC CGAGAGCAAC 
CATGTGAGCT TCGTGTATGA GGAGAACGAG TGTTCCCTAG TGCTGCTCAG CGCAGGAAGC 
CAGGATGGAG GCGTCTATAC CTGTACCGCC CGGAACCTGG CAGGCGAAGT CTCCTGCAAG 
GCGGAGCTGT CTGTGCTTTC AGCTCAGACA GCCATGGAGG TCGAGGGGGT CGGAGAGGAT 
GAGGAACACC GAGGAAGAAG ACTTAGCGAC TACTACGACA TCCACCAAGA GATTGGCAGG 
GGTGCCTTCT CCTACCTGCG GCGTGTAGTG GAGAGGAGCT CTGGCCTGGA GTTTGCAGCC 
AAGTTCATCC CTAGCCAGGC TAAGCCTAAA GCATCAGCAA GACGCGAGGC GCGCTTGCTG 
GCCCGGCTCC AGCATGGCTG TGTGCTCTAC TTCCACGAAG CCTTTGAGAG GCGCAGGGGA 
CTTGTCATAG TCACGGAGCT CTGCACAGAA GAGCTTCTAG AGCGCATGGC CAGGAAGCCC 
ACCGTGTGTG AGTCTGAGAC TCGGACCTAT ATGCGTCAAG TGCTAGAGGG GATATGCTAT 
CTGCATCAGA GCCATGTGCT GCACCTGGAT GTCAAGCCGG AGAACCTGCT TGTGTGGGAT 
GGTGCAGGGG GTGAAGAGCA GGTGCGCATC TGTGACTTCG GGAACGCCCA GGAACTCACT 
CCGGGAGAGC CCCAGTACTG TCAGTATGGC ACGCCAGAGT TTGTAGCACC TGAGATTGTC 
AACCAGAGCC CTGTGTCTGG AGTCACTGAC ATCTGGCCCG TGGGCGTCGT TGCCTTTCTC 
TGTTTGACGG GCATCTCCCC ATTTGTTGGG GAGAATGACC GGACAACACT CATGAATATC 
CGAAACTACA ACGTGGCATT TGAGGAGACT ACGTTCCTGA GTCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGCT GGTGCAGGAT CGACTGAGAC CTACTGCAGA AGAGACATTG 
GAACATCCTT GGTTCAAAAC AGAAGCAAAG GGTGCAGAGG TGAGCACAGA TCACCTGAAG 
CTCTTCCTGT CACGGAGGAG GTGGCAGCGC TCTCAAATCA GCTACAAGTG CCACCTGGTG 
CTGCGCCCCA TTCCGGAACT GCTACGGGCT CCCCCGGAGC GGGTGTGGGT GGCCATGCCT 
AGAAGACAAC CCCCGAGTGG GGGACTCTCA TCCTCTTCAG ACTCAGAAGA GGAAGAGTTG 
GAGGAACTTC CCTCAGTACC CCGCCCCCTG CAGCCTGAGT TCTCAGGTTC TCGGGTATCC 
CTCACCGACA TCCCCACCGA AGATGAGGCT CTGGGGACCC CGGAAGCTGG GGCTGCCACC 
CCCATGGACT GGCAAGAGCA GGAAAGGACT CCCTCTAAGG ACCAGGAGGC CCCCAGCCCT 
GAGGCACTCC CCTCTCCAGG CCAGGAGTCC CCTGATGGGC CTAGCCCCAG GCGGCCAGAG 
CTCCGCAGGG GTAGCTCTGC TGAGAGTGCC CTGCCCCGAG TCGGGTCGCG GGAACCGGGC 
CGGAGCTTGC ACAAGGCAGC ATCCGTGGAG CTGCCGCAGC GCAGGAGCCC CAGCCCGGGT 
GCCACCCGCC TGACTCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGTCAGC GGCTGCTGAG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGA 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCA CGGGATCCCA GGATGGCAAG GGCTGCCTCC 
AGCGAGGCTG CCCCCCACCA CCAGCCCCCT CCGGAGTCCC GGGGTCTGCA AAAGAGCAGC 
AGCTTCTCTC AGGGCGAGGC AGAGCCCCGG GGTCGGCACC GCCGCGCGGG GGCACCCCTC 
GAAATCCCCG TGGCCAGACT TGGGGCTCGC AGGCTGCAGG AGTCTCCCTC CTTATCTGCC 
CTTAGCGAGA CCCAGCCACC GAGCCCTGCG AGGCCCAGCG TCCCCAAACT CAGTATCACC 
AAGTCCCCGG AACCTTCCGC TGTCACATCC AGAGACTCTC CCCAGCCCCC GGAACCTCAG 
CCTGTCCCAG AAAAGGTCCC TGAGCCCAAA CCAGAACCAG TCCGAGCTGC AAAACCAGCA 
CAACCCCCTT TGGCTCTGCA AATGCCAACG CAGCCTCTCA CCCCCTATGC CCAGATCATG 
CAGTCGCTCC AGCTGTCCAG TCCCACCCTG TCCCCACAGG ACCCCGCTGT GCCTCCATCA 
GAGCCCAAGC CCCACGCGGC TGTGTTTGCC AGGGTGGCTT CCCCACCACC CGGAGTCTCT 
GAGAAGCGCG TGCCTTCGGC CAGAACTCCC CCAGTGCTAG CCGAGAAAGC CCGAGTCCCC 
ACGGTGCCCC CCAGGCCAGG CAGCAGCCTC AGCGGCAGCA TCGAAAACCT TGAATCAGAG 
GCTGTGTTCG AGGCTAAGTT CAAACGCAGC CGCGAATCGC CACTGTCGCG GGGCCTGAGG 
CTACTGAGCC GCTCGCGCTC GGAGGAGCGC GGTCCCTTCC GTGGGGCGGA GGACGACGGC 
ATATACCGGC CCAGTCCGGC AGGAACGCCG CTAGAGCTGG TGCGACGGCC CGAGCGCTCG 
CGCTCCGTGC AGGACCTCAG GGTAGCCGGA GAGCCGGGCC TTGTGCGTCG CCTTTCGCTG 
TCCCTGTCGC AGAAGCTGCG GCGGACACCG CCGGGACAGC GACACCCTGC CTGGGAGTCC 
CGCAGCGGAG ACGGGGAGAG CTCCGAGGGC GGCAGCTCAG CTCGGGCATC CCCAGTGCTG 
GCGGTACGCA GGAGACTCAG CTCCACGCTG GAGCGTCTGT CCAGCCGTTT GCAGCGCAGC 
GGCAGCAGCG AGGACTCCGG GGGCGCCTCG GGTCGCAGCA CGCCGCTGTT TGGACGGCTG 
CGCAGGGCTA CTTCGGAAGG GGAGAGCCTG CGACGCCTCG GTGTTCCGCA CAATCAGCTG 
GGCTCTCAGA CTGGTGCCAC TACACCTTCC GCTGAGTCTC TGGGCTCAGA GGCCAGTGGC 
ACATCGGGTT CTTCAGCTCC TGGAGAAAGC CGAAGCCGGC ACCGGTGGGG CCTTTCCAGG 
CTGCGCAAGG ACAAGGGATT ATCTCAGCCA AACCTCTCCT CCAGTGTCCA AGAGGACTTG 
GGTCACCAGT ATGTGCCCAG TGAGTCAGAT TTCCCCCCAG TCTTCCACAT CAAACTCAAG 
GACCAGGTGC TGTTGGAGGG GGAAGCAGCC ACCCTGCTCT GCCTACCCGC AGCCTGCCCA 
GCACCCCGCA TCTCCTGGAT GAAAGATAAG CAGTCCCTGC GTTCAGAGCC CTCAGTGGTC 
ATTGTGTCCT GCAAAGATGG ACGGCAGCTG CTGAGCATAC CCCGGGCCGG CAAGCGGCAT 
GCTGGGCTCT ATGAGTGCTC AGCCACCAAT GTCCTGGGCA GCATTACCAG CTCCTGTACT 
GTGGCCGTGG CCCGCATTCC AGGGAAGCTA GCTCCTCCAG AGGTGCCCCA GACCTACCAT 
GACACAGCAC TAGTAGTGTG GAAGCCAGGA GACGGTCGGG CACCTTGCAC ATACACTCTG 
GAGCGGCGGG TGGATGGGGA ATCTGTCTGG CACCCTGTGA GCTCAGGCAT CCCCGACTGT 
TATTACAATG TGACTCAGCT GCCTGTCGGC GTGACCGTGC GGTTCCGTGT GGCCTGTTCC 
AATCGTGCTG GGCAGGGGCC CTTCAGCAAC CCTTCTGAGA AGGTCTTCAT CCGAGGCACT 
CCAGATTCTC CTGCTCAGCC AGCTGCTGCT CCCCGAGATG CCCCTGTTAC CTCAGGGCCA 
ACCAGGGCCC CACCGCCTGA CTCTCCTACC TCACTGGCCC CCACCCCCGC TCTTGCGCCC 
CCAGCCTCCC AGGCATCCAC TCTCAGCCCC TCAACTTCCT CCATGTCTGC CAACCAGGCC 
TTGTCCTCAC TCAAGGCTGT GGGTCCACCA CCCGCAACCC CTCCACGAAA GCACAGGGGC 
CTTCTGGCCA CCCAGCAAGC AGAGCCATCC CCACCCAGTA TCGTGGTCAC CCCAAGTGAA 
CCCAGGTCTT TTGTCCCTGA CACTGGAACC CTGACCCCAA CCTCTAGTCC TCAAGGGGTT 
AAACCAGCAC CTTCCTCAAC TTCCTTGTAT ATGGTGACTT CCTTCGTGTC TGCACCTCCA 
GCCCCCCAGG CCCCAGCCCC TGAGCCCCCT CCTGAGCCCA CCAAGGTGAC TGTGCGGAGC 
CTCAGCCCAG CCAAGGAGGT GGTCAGCTCC CCAACTCCTG AGAGCACCAC TCTTCGGCAG 
GGTCCCCCTC AGAAACCCTA CACCTTCCTG GAGGAGAAGG CCAGGGGCCG CTTTGGCGTT 
GTGCGGTCAT GCCGGGAGAA TGCTACGGGC CGAACGTTTG TCGCCAAGAT CGTGCCGTAC 
GCCGCGGAGG GCAAGCGACG CGTGCTGCAG GAATACGAGG TGCTTCGGAC GCTGCACCAC 
GAGCGGCTCA TGTCCCTGCA CGAGGCCTAC ATCACCCCTC GGTACCTCGT GCTTATCGCT 
GAGAGCTGTG GCAACCGGGA ACTCCTCTGT GGGCTCAGCG ACAGATTCCG GTATTCAGAA 
GATGACGTGG CCACCTATGT GGTGCAGCTG CTACAAGGCC TGGACTACCT CCACGGCCAC 
CACGTGCTAC ACCTGGACAT CAAGCCGGAC AATCTACTGT TGGCCGCTGA CAATGCCCTT 
AAAATTGTGG ACTTCGGCAG TGCTCAGCCC TATAACCCTC AGGCCCTGAA GCCACTTGGC 
CACCGCACAG GCACTCTGGA GTTCATGGCT CCTGAGATGG TGAAGGGAGA CCCCATTGGC 
TCTGCCACGG ACATCTGGGG AGCGGGTGTG CTCACCTACA TCATGCTTAG TGGGTACTCC 
CCATTCTATG AGCCAGACCC CCAGGAAACA GAGGCTCGGA TTGTTGGGGG TCGCTTTGAT 
GCCTTCCAGT TGTATCCTAA CACATCCCAG AGTGCCACCC TCTTCTTGAG AAAGGTCCTC 
TCAGTACATC CCTGGAGCCG GCCCTCTCTG CAGGACTGCT TGGCCCACCC ATGGCTGCAA 
GATGCCTACC TGATGAAGCT GCGCCGCCAG ACACTCACCT TCACCACCAA CCGGCTCAAG 
GAATTCCTGG GCGAGCAGCG GCGACGTCGG GCTGAGGCTG CTACCCGTCA CAAGGTGCTG 
CTCCGCTCCT ACCCTGGCAG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496467.1
CDS	207..7433
Translation	MKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_006496404.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496404.3

ORF Insert Sequence:

ATGAAGCTCA GTCCCAGCCA GGATCATGAT TCCTCCGACT CTTCTTCCAA GGCACCCCCA 
ACGTTCAAGG TCTCACTCAT GGACCAATCG GTGAGAGAAG GTCAAGATGT CATTATGAGC 
ATCCGTGTGC AGGGAGAGCC CAAGCCTGTG GTTTCCTGGC TGAGGAATCG ACAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCAGAGGAG GCCGAGGGTG GGCTCTGCCG CTTGAGGATC 
CTGGCTGCTG AACGGGGCGA TGCTGGTTTC TACACATGCA AGGCGGTCAA CGAATATGGC 
GCTCGGCAGT GCGAGGCCCG CCTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCAG GTCCAGCTGA CGTCGAGGTG GACTGGCTGT GCCGGGGCCG CCTGCTGCAG 
CCTGCGCTGC TCAAATGCAA GATGCATTTT GATGGCCGCA AGTGCAAGCT GCTGCTCACC 
TCTGTGCACG AGGACGACAG TGGTGTCTAC ACCTGCAAGC TGAGCACAGC CAAAGATGAA 
CTGACCTGCA GTGCCCGGCT GACGGTTCGG CCGTCGCTGG CGCCTCTCTT CACCCGGTTG 
CTGGAAGATG TGGAGGTGTT GGAAGGCCGT GCTGCCCGCT TGGATTGCAA GATCAGCGGT 
ACCCCGCCTC CCTCCGTTAC CTGGACTCAT TTTGGCCACC CCGTGAATGA GGGTGACAAC 
TTGCGGCTTC AACAGGATGG AGGTCTACAC TCACTGCACA TTGCCCGGGT GGGCAGTGAG 
GACGAAGGAC TCTATGAAGT CAGTGCCACC AACACTCATG GCCAGGCCCA CTGCTCCGCC 
CAGCTGTATG TGGAGGAGCC TCGGACAGCT GCCTCTGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCTA TCCCTGAGGA GCCGGAGCAC GGAGATCTAG AGAGGCTGTC TATTCCTGAC 
TTCCTACGGC CACTGCAAGA TCTGGAAGTG GGACTAGCTA AGGAGGCCAT GCTGGAGTGC 
CAAGTGACCG GCCTGCCCTA TCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATTCAA 
AGCAGTGACG ACCGACGCAT GACACAGTAC AGGGACATAC ATCGCCTGGT GTTCCCAGCT 
GTGGGGCCTC AGCATGCTGG TGTCTATAAG AGTGTAATCG CCAACAAGCT GGGCAAAGCG 
GCCTGCTACG CCCATCTCTA TGTCACAGAT GTGGTTCCAG GCCCTCCAGA TGGTGCTCCG 
GAAGTAGTAG CTGTGACCGG AAGGATGGTC ACACTATCGT GGAACCCACC CAGGAGTCTG 
GACATGGCTA TTGACCCAGA TTCCCTAACA TACACTGTGC AGCACCAGGT GTTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCTG CGTGGGCAGC CACAGGGCTG 
AAGAAGGGGA TCCAGCACAT CTTCCGGGTC CTCAGCTCCA GTGGCAAGAG CAGCAGCAAG 
CCCTCAGCAC CGTCGGAACC CGTGCAGCTG CTAGAGCATG GCCCACCCCT GGAAGAGGCG 
CCTGCTGTGC TGGACAAGCA GGACATTGTG TATGTGGTAG AGGGCCAGCC TGCCTGTGTC 
ACCGTCACTT TTAACCATGT GGAGGCCCAG GTTGTCTGGA GGAGCTGCCG GGGAGCCCTC 
CTCGAGGCGC GCACAGGTGT GTATGAACTG AGTCAGCCAG ATGACGACCA GTATTGTCTG 
CGGATCTGCC GGGTGAGCCG ACGAGACCTG GGACCCCTTA CTTGCAGTGC CCGAAACCGC 
CACGGCACAA AGGCCTGCTC GGTCACCCTG GAGCTGGCAG AGGCACCTCG GTTTGAGTCT 
ATCATGGAAG ATGTGGAGGT GGGGCCTGGA GAAACCGCTC GCTTTGCTGT GGTAGTTGAG 
GGGAAACCAC TGCCAGACAT CATGTGGTAC AAGGATGAGG TGCTGCTGGC CGAGAGCAAC 
CATGTGAGCT TCGTGTATGA GGAGAACGAG TGTTCCCTAG TGCTGCTCAG CGCAGGAAGC 
CAGGATGGAG GCGTCTATAC CTGTACCGCC CGGAACCTGG CAGGCGAAGT CTCCTGCAAG 
GCGGAGCTGT CTGTGCTTTC AGCTCAGACA GCCATGGAGG TCGAGGGGGT CGGAGAGGAT 
GAGGAACACC GAGGAAGAAG ACTTAGCGAC TACTACGACA TCCACCAAGA GATTGGCAGG 
GGTGCCTTCT CCTACCTGCG GCGTGTAGTG GAGAGGAGCT CTGGCCTGGA GTTTGCAGCC 
AAGTTCATCC CTAGCCAGGC TAAGCCTAAA GCATCAGCAA GACGCGAGGC GCGCTTGCTG 
GCCCGGCTCC AGCATGGCTG TGTGCTCTAC TTCCACGAAG CCTTTGAGAG GCGCAGGGGA 
CTTGTCATAG TCACGGAGCT CTGCACAGAA GAGCTTCTAG AGCGCATGGC CAGGAAGCCC 
ACCGTGTGTG AGTCTGAGAC TCGGACCTAT ATGCGTCAAG TGCTAGAGGG GATATGCTAT 
CTGCATCAGA GCCATGTGCT GCACCTGGAT GTCAAGCCGG AGAACCTGCT TGTGTGGGAT 
GGTGCAGGGG GTGAAGAGCA GGTGCGCATC TGTGACTTCG GGAACGCCCA GGAACTCACT 
CCGGGAGAGC CCCAGTACTG TCAGTATGGC ACGCCAGAGT TTGTAGCACC TGAGATTGTC 
AACCAGAGCC CTGTGTCTGG AGTCACTGAC ATCTGGCCCG TGGGCGTCGT TGCCTTTCTC 
TGTTTGACGG GCATCTCCCC ATTTGTTGGG GAGAATGACC GGACAACACT CATGAATATC 
CGAAACTACA ACGTGGCATT TGAGGAGACT ACGTTCCTGA GTCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGCT GGTGCAGGAT CGACTGAGAC CTACTGCAGA AGAGACATTG 
GAACATCCTT GGTTCAAAAC AGAAGCAAAG GGTGCAGAGG TGAGCACAGA TCACCTGAAG 
CTCTTCCTGT CACGGAGGAG GTGGCAGCGC TCTCAAATCA GCTACAAGTG CCACCTGGTG 
CTGCGCCCCA TTCCGGAACT GCTACGGGCT CCCCCGGAGC GGGTGTGGGT GGCCATGCCT 
AGAAGACAAC CCCCGAGTGG GGGACTCTCA TCCTCTTCAG ACTCAGAAGA GGAAGAGTTG 
GAGGAACTTC CCTCAGTACC CCGCCCCCTG CAGCCTGAGT TCTCAGGTTC TCGGGTATCC 
CTCACCGACA TCCCCACCGA AGATGAGGCT CTGGGGACCC CGGAAGCTGG GGCTGCCACC 
CCCATGGACT GGCAAGAGCA GGAAAGGACT CCCTCTAAGG ACCAGGAGGC CCCCAGCCCT 
GAGGCACTCC CCTCTCCAGG CCAGGAGTCC CCTGATGGGC CTAGCCCCAG GCGGCCAGAG 
CTCCGCAGGG GTAGCTCTGC TGAGAGTGCC CTGCCCCGAG TCGGGTCGCG GGAACCGGGC 
CGGAGCTTGC ACAAGGCAGC ATCCGTGGAG CTGCCGCAGC GCAGGAGCCC CAGCCCGGGT 
GCCACCCGCC TGACTCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGTCAGC GGCTGCTGAG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGA 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCA CGGGATCCCA GGATGGCAAG GGCTGCCTCC 
AGCGAGGCTG CCCCCCACCA CCAGCCCCCT CCGGAGTCCC GGGGTCTGCA AAAGAGCAGC 
AGCTTCTCTC AGGGCGAGGC AGAGCCCCGG GGTCGGCACC GCCGCGCGGG GGCACCCCTC 
GAAATCCCCG TGGCCAGACT TGGGGCTCGC AGGCTGCAGG AGTCTCCCTC CTTATCTGCC 
CTTAGCGAGA CCCAGCCACC GAGCCCTGCG AGGCCCAGCG TCCCCAAACT CAGTATCACC 
AAGTCCCCGG AACCTTCCGC TGTCACATCC AGAGACTCTC CCCAGCCCCC GGAACCTCAG 
CCTGTCCCAG AAAAGGTCCC TGAGCCCAAA CCAGAACCAG TCCGAGCTGC AAAACCAGCA 
CAACCCCCTT TGGCTCTGCA AATGCCAACG CAGCCTCTCA CCCCCTATGC CCAGATCATG 
CAGTCGCTCC AGCTGTCCAG TCCCACCCTG TCCCCACAGG ACCCCGCTGT GCCTCCATCA 
GAGCCCAAGC CCCACGCGGC TGTGTTTGCC AGGGTGGCTT CCCCACCACC CGGAGTCTCT 
GAGAAGCGCG TGCCTTCGGC CAGAACTCCC CCAGTGCTAG CCGAGAAAGC CCGAGTCCCC 
ACGGTGCCCC CCAGGCCAGG CAGCAGCCTC AGCGGCAGCA TCGAAAACCT TGAATCAGAG 
GCTGTGTTCG AGGCTAAGTT CAAACGCAGC CGCGAATCGC CACTGTCGCG GGGCCTGAGG 
CTACTGAGCC GCTCGCGCTC GGAGGAGCGC GGTCCCTTCC GTGGGGCGGA GGACGACGGC 
ATATACCGGC CCAGTCCGGC AGGAACGCCG CTAGAGCTGG TGCGACGGCC CGAGCGCTCG 
CGCTCCGTGC AGGACCTCAG GGTAGCCGGA GAGCCGGGCC TTGTGCGTCG CCTTTCGCTG 
TCCCTGTCGC AGAAGCTGCG GCGGACACCG CCGGGACAGC GACACCCTGC CTGGGAGTCC 
CGCAGCGGAG ACGGGGAGAG CTCCGAGGGC GGCAGCTCAG CTCGGGCATC CCCAGTGCTG 
GCGGTACGCA GGAGACTCAG CTCCACGCTG GAGCGTCTGT CCAGCCGTTT GCAGCGCAGC 
GGCAGCAGCG AGGACTCCGG GGGCGCCTCG GGTCGCAGCA CGCCGCTGTT TGGACGGCTG 
CGCAGGGCTA CTTCGGAAGG GGAGAGCCTG CGACGCCTCG GTGTTCCGCA CAATCAGCTG 
GGCTCTCAGA CTGGTGCCAC TACACCTTCC GCTGAGTCTC TGGGCTCAGA GGCCAGTGGC 
ACATCGGGTT CTTCAGCTCC TGGAGAAAGC CGAAGCCGGC ACCGGTGGGG CCTTTCCAGG 
CTGCGCAAGG ACAAGGGATT ATCTCAGCCA AACCTCTCCT CCAGTGTCCA AGAGGACTTG 
GGTCACCAGT ATGTGCCCAG TGAGTCAGAT TTCCCCCCAG TCTTCCACAT CAAACTCAAG 
GACCAGGTGC TGTTGGAGGG GGAAGCAGCC ACCCTGCTCT GCCTACCCGC AGCCTGCCCA 
GCACCCCGCA TCTCCTGGAT GAAAGATAAG CAGTCCCTGC GTTCAGAGCC CTCAGTGGTC 
ATTGTGTCCT GCAAAGATGG ACGGCAGCTG CTGAGCATAC CCCGGGCCGG CAAGCGGCAT 
GCTGGGCTCT ATGAGTGCTC AGCCACCAAT GTCCTGGGCA GCATTACCAG CTCCTGTACT 
GTGGCCGTGG CCCGCATTCC AGGGAAGCTA GCTCCTCCAG AGGTGCCCCA GACCTACCAT 
GACACAGCAC TAGTAGTGTG GAAGCCAGGA GACGGTCGGG CACCTTGCAC ATACACTCTG 
GAGCGGCGGG TGGATGGGGA ATCTGTCTGG CACCCTGTGA GCTCAGGCAT CCCCGACTGT 
TATTACAATG TGACTCAGCT GCCTGTCGGC GTGACCGTGC GGTTCCGTGT GGCCTGTTCC 
AATCGTGCTG GGCAGGGGCC CTTCAGCAAC CCTTCTGAGA AGGTCTTCAT CCGAGGCACT 
CCAGATTCTC CTGCTCAGCC AGCTGCTGCT CCCCGAGATG CCCCTGTTAC CTCAGGGCCA 
ACCAGGGCCC CACCGCCTGA CTCTCCTACC TCACTGGCCC CCACCCCCGC TCTTGCGCCC 
CCAGCCTCCC AGGCATCCAC TCTCAGCCCC TCAACTTCCT CCATGTCTGC CAACCAGGCC 
TTGTCCTCAC TCAAGGCTGT GGGTCCACCA CCCGCAACCC CTCCACGAAA GCACAGGGGC 
CTTCTGGCCA CCCAGCAAGC AGAGCCATCC CCACCCAGTA TCGTGGTCAC CCCAAGTGAA 
CCCAGGTCTT TTGTCCCTGA CACTGGAACC CTGACCCCAA CCTCTAGTCC TCAAGGGGTT 
AAACCAGCAC CTTCCTCAAC TTCCTTGTAT ATGGTGACTT CCTTCGTGTC TGCACCTCCA 
GCCCCCCAGG CCCCAGCCCC TGAGCCCCCT CCTGAGCCCA CCAAGGTGAC TGTGCGGAGC 
CTCAGCCCAG CCAAGGAGGT GGTCAGCTCC CCAACTCCTG AGAGCACCAC TCTTCGGCAG 
GGTCCCCCTC AGAAACCCTA CACCTTCCTG GAGGAGAAGG CCAGGGGCCG CTTTGGCGTT 
GTGCGGTCAT GCCGGGAGAA TGCTACGGGC CGAACGTTTG TCGCCAAGAT CGTGCCGTAC 
GCCGCGGAGG GCAAGCGACG CGTGCTGCAG GAATACGAGG TGCTTCGGAC GCTGCACCAC 
GAGCGGCTCA TGTCCCTGCA CGAGGCCTAC ATCACCCCTC GGTACCTCGT GCTTATCGCT 
GAGAGCTGTG GCAACCGGGA ACTCCTCTGT GGGCTCAGCG ACAGATTCCG GTATTCAGAA 
GATGACGTGG CCACCTATGT GGTGCAGCTG CTACAAGGCC TGGACTACCT CCACGGCCAC 
CACGTGCTAC ACCTGGACAT CAAGCCGGAC AATCTACTGT TGGCCGCTGA CAATGCCCTT 
AAAATTGTGG ACTTCGGCAG TGCTCAGCCC TATAACCCTC AGGCCCTGAA GCCACTTGGC 
CACCGCACAG GCACTCTGGA GTTCATGGCT CCTGAGATGG TGAAGGGAGA CCCCATTGGC 
TCTGCCACGG ACATCTGGGG AGCGGGTGTG CTCACCTACA TCATGCTTAG TGGGTACTCC 
CCATTCTATG AGCCAGACCC CCAGGAAACA GAGGCTCGGA TTGTTGGGGG TCGCTTTGAT 
GCCTTCCAGT TGTATCCTAA CACATCCCAG AGTGCCACCC TCTTCTTGAG AAAGGTCCTC 
TCAGTACATC CCTGGAGCCG GCCCTCTCTG CAGGACTGCT TGGCCCACCC ATGGCTGCAA 
GATGCCTACC TGATGAAGCT GCGCCGCCAG ACACTCACCT TCACCACCAA CCGGCTCAAG 
GAATTCCTGG GCGAGCAGCG GCGACGTCGG GCTGAGGCTG CTACCCGTCA CAAGGTGCTG 
CTCCGCTCCT ACCCTGGCAG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_006496403.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496466.1 Mus musculus SPEG complex locus (Speg), transcript variant X13, mRNA.

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CloneID	OMu27706
Clone ID Related Accession (Same CDS sequence)	XM_006496404.4 , XM_006496404.3 , XM_006496403.3 , XM_006496403.4
Accession Version	XM_006496403.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496403.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCA GTCCCAGCCA GGATCATGAT TCCTCCGACT CTTCTTCCAA GGCACCCCCA 
ACGTTCAAGG TCTCACTCAT GGACCAATCG GTGAGAGAAG GTCAAGATGT CATTATGAGC 
ATCCGTGTGC AGGGAGAGCC CAAGCCTGTG GTTTCCTGGC TGAGGAATCG ACAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCAGAGGAG GCCGAGGGTG GGCTCTGCCG CTTGAGGATC 
CTGGCTGCTG AACGGGGCGA TGCTGGTTTC TACACATGCA AGGCGGTCAA CGAATATGGC 
GCTCGGCAGT GCGAGGCCCG CCTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCAG GTCCAGCTGA CGTCGAGGTG GACTGGCTGT GCCGGGGCCG CCTGCTGCAG 
CCTGCGCTGC TCAAATGCAA GATGCATTTT GATGGCCGCA AGTGCAAGCT GCTGCTCACC 
TCTGTGCACG AGGACGACAG TGGTGTCTAC ACCTGCAAGC TGAGCACAGC CAAAGATGAA 
CTGACCTGCA GTGCCCGGCT GACGGTTCGG CCGTCGCTGG CGCCTCTCTT CACCCGGTTG 
CTGGAAGATG TGGAGGTGTT GGAAGGCCGT GCTGCCCGCT TGGATTGCAA GATCAGCGGT 
ACCCCGCCTC CCTCCGTTAC CTGGACTCAT TTTGGCCACC CCGTGAATGA GGGTGACAAC 
TTGCGGCTTC AACAGGATGG AGGTCTACAC TCACTGCACA TTGCCCGGGT GGGCAGTGAG 
GACGAAGGAC TCTATGAAGT CAGTGCCACC AACACTCATG GCCAGGCCCA CTGCTCCGCC 
CAGCTGTATG TGGAGGAGCC TCGGACAGCT GCCTCTGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCTA TCCCTGAGGA GCCGGAGCAC GGAGATCTAG AGAGGCTGTC TATTCCTGAC 
TTCCTACGGC CACTGCAAGA TCTGGAAGTG GGACTAGCTA AGGAGGCCAT GCTGGAGTGC 
CAAGTGACCG GCCTGCCCTA TCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATTCAA 
AGCAGTGACG ACCGACGCAT GACACAGTAC AGGGACATAC ATCGCCTGGT GTTCCCAGCT 
GTGGGGCCTC AGCATGCTGG TGTCTATAAG AGTGTAATCG CCAACAAGCT GGGCAAAGCG 
GCCTGCTACG CCCATCTCTA TGTCACAGAT GTGGTTCCAG GCCCTCCAGA TGGTGCTCCG 
GAAGTAGTAG CTGTGACCGG AAGGATGGTC ACACTATCGT GGAACCCACC CAGGAGTCTG 
GACATGGCTA TTGACCCAGA TTCCCTAACA TACACTGTGC AGCACCAGGT GTTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCTG CGTGGGCAGC CACAGGGCTG 
AAGAAGGGGA TCCAGCACAT CTTCCGGGTC CTCAGCTCCA GTGGCAAGAG CAGCAGCAAG 
CCCTCAGCAC CGTCGGAACC CGTGCAGCTG CTAGAGCATG GCCCACCCCT GGAAGAGGCG 
CCTGCTGTGC TGGACAAGCA GGACATTGTG TATGTGGTAG AGGGCCAGCC TGCCTGTGTC 
ACCGTCACTT TTAACCATGT GGAGGCCCAG GTTGTCTGGA GGAGCTGCCG GGGAGCCCTC 
CTCGAGGCGC GCACAGGTGT GTATGAACTG AGTCAGCCAG ATGACGACCA GTATTGTCTG 
CGGATCTGCC GGGTGAGCCG ACGAGACCTG GGACCCCTTA CTTGCAGTGC CCGAAACCGC 
CACGGCACAA AGGCCTGCTC GGTCACCCTG GAGCTGGCAG AGGCACCTCG GTTTGAGTCT 
ATCATGGAAG ATGTGGAGGT GGGGCCTGGA GAAACCGCTC GCTTTGCTGT GGTAGTTGAG 
GGGAAACCAC TGCCAGACAT CATGTGGTAC AAGGATGAGG TGCTGCTGGC CGAGAGCAAC 
CATGTGAGCT TCGTGTATGA GGAGAACGAG TGTTCCCTAG TGCTGCTCAG CGCAGGAAGC 
CAGGATGGAG GCGTCTATAC CTGTACCGCC CGGAACCTGG CAGGCGAAGT CTCCTGCAAG 
GCGGAGCTGT CTGTGCTTTC AGCTCAGACA GCCATGGAGG TCGAGGGGGT CGGAGAGGAT 
GAGGAACACC GAGGAAGAAG ACTTAGCGAC TACTACGACA TCCACCAAGA GATTGGCAGG 
GGTGCCTTCT CCTACCTGCG GCGTGTAGTG GAGAGGAGCT CTGGCCTGGA GTTTGCAGCC 
AAGTTCATCC CTAGCCAGGC TAAGCCTAAA GCATCAGCAA GACGCGAGGC GCGCTTGCTG 
GCCCGGCTCC AGCATGGCTG TGTGCTCTAC TTCCACGAAG CCTTTGAGAG GCGCAGGGGA 
CTTGTCATAG TCACGGAGCT CTGCACAGAA GAGCTTCTAG AGCGCATGGC CAGGAAGCCC 
ACCGTGTGTG AGTCTGAGAC TCGGACCTAT ATGCGTCAAG TGCTAGAGGG GATATGCTAT 
CTGCATCAGA GCCATGTGCT GCACCTGGAT GTCAAGCCGG AGAACCTGCT TGTGTGGGAT 
GGTGCAGGGG GTGAAGAGCA GGTGCGCATC TGTGACTTCG GGAACGCCCA GGAACTCACT 
CCGGGAGAGC CCCAGTACTG TCAGTATGGC ACGCCAGAGT TTGTAGCACC TGAGATTGTC 
AACCAGAGCC CTGTGTCTGG AGTCACTGAC ATCTGGCCCG TGGGCGTCGT TGCCTTTCTC 
TGTTTGACGG GCATCTCCCC ATTTGTTGGG GAGAATGACC GGACAACACT CATGAATATC 
CGAAACTACA ACGTGGCATT TGAGGAGACT ACGTTCCTGA GTCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGCT GGTGCAGGAT CGACTGAGAC CTACTGCAGA AGAGACATTG 
GAACATCCTT GGTTCAAAAC AGAAGCAAAG GGTGCAGAGG TGAGCACAGA TCACCTGAAG 
CTCTTCCTGT CACGGAGGAG GTGGCAGCGC TCTCAAATCA GCTACAAGTG CCACCTGGTG 
CTGCGCCCCA TTCCGGAACT GCTACGGGCT CCCCCGGAGC GGGTGTGGGT GGCCATGCCT 
AGAAGACAAC CCCCGAGTGG GGGACTCTCA TCCTCTTCAG ACTCAGAAGA GGAAGAGTTG 
GAGGAACTTC CCTCAGTACC CCGCCCCCTG CAGCCTGAGT TCTCAGGTTC TCGGGTATCC 
CTCACCGACA TCCCCACCGA AGATGAGGCT CTGGGGACCC CGGAAGCTGG GGCTGCCACC 
CCCATGGACT GGCAAGAGCA GGAAAGGACT CCCTCTAAGG ACCAGGAGGC CCCCAGCCCT 
GAGGCACTCC CCTCTCCAGG CCAGGAGTCC CCTGATGGGC CTAGCCCCAG GCGGCCAGAG 
CTCCGCAGGG GTAGCTCTGC TGAGAGTGCC CTGCCCCGAG TCGGGTCGCG GGAACCGGGC 
CGGAGCTTGC ACAAGGCAGC ATCCGTGGAG CTGCCGCAGC GCAGGAGCCC CAGCCCGGGT 
GCCACCCGCC TGACTCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGTCAGC GGCTGCTGAG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGA 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCA CGGGATCCCA GGATGGCAAG GGCTGCCTCC 
AGCGAGGCTG CCCCCCACCA CCAGCCCCCT CCGGAGTCCC GGGGTCTGCA AAAGAGCAGC 
AGCTTCTCTC AGGGCGAGGC AGAGCCCCGG GGTCGGCACC GCCGCGCGGG GGCACCCCTC 
GAAATCCCCG TGGCCAGACT TGGGGCTCGC AGGCTGCAGG AGTCTCCCTC CTTATCTGCC 
CTTAGCGAGA CCCAGCCACC GAGCCCTGCG AGGCCCAGCG TCCCCAAACT CAGTATCACC 
AAGTCCCCGG AACCTTCCGC TGTCACATCC AGAGACTCTC CCCAGCCCCC GGAACCTCAG 
CCTGTCCCAG AAAAGGTCCC TGAGCCCAAA CCAGAACCAG TCCGAGCTGC AAAACCAGCA 
CAACCCCCTT TGGCTCTGCA AATGCCAACG CAGCCTCTCA CCCCCTATGC CCAGATCATG 
CAGTCGCTCC AGCTGTCCAG TCCCACCCTG TCCCCACAGG ACCCCGCTGT GCCTCCATCA 
GAGCCCAAGC CCCACGCGGC TGTGTTTGCC AGGGTGGCTT CCCCACCACC CGGAGTCTCT 
GAGAAGCGCG TGCCTTCGGC CAGAACTCCC CCAGTGCTAG CCGAGAAAGC CCGAGTCCCC 
ACGGTGCCCC CCAGGCCAGG CAGCAGCCTC AGCGGCAGCA TCGAAAACCT TGAATCAGAG 
GCTGTGTTCG AGGCTAAGTT CAAACGCAGC CGCGAATCGC CACTGTCGCG GGGCCTGAGG 
CTACTGAGCC GCTCGCGCTC GGAGGAGCGC GGTCCCTTCC GTGGGGCGGA GGACGACGGC 
ATATACCGGC CCAGTCCGGC AGGAACGCCG CTAGAGCTGG TGCGACGGCC CGAGCGCTCG 
CGCTCCGTGC AGGACCTCAG GGTAGCCGGA GAGCCGGGCC TTGTGCGTCG CCTTTCGCTG 
TCCCTGTCGC AGAAGCTGCG GCGGACACCG CCGGGACAGC GACACCCTGC CTGGGAGTCC 
CGCAGCGGAG ACGGGGAGAG CTCCGAGGGC GGCAGCTCAG CTCGGGCATC CCCAGTGCTG 
GCGGTACGCA GGAGACTCAG CTCCACGCTG GAGCGTCTGT CCAGCCGTTT GCAGCGCAGC 
GGCAGCAGCG AGGACTCCGG GGGCGCCTCG GGTCGCAGCA CGCCGCTGTT TGGACGGCTG 
CGCAGGGCTA CTTCGGAAGG GGAGAGCCTG CGACGCCTCG GTGTTCCGCA CAATCAGCTG 
GGCTCTCAGA CTGGTGCCAC TACACCTTCC GCTGAGTCTC TGGGCTCAGA GGCCAGTGGC 
ACATCGGGTT CTTCAGCTCC TGGAGAAAGC CGAAGCCGGC ACCGGTGGGG CCTTTCCAGG 
CTGCGCAAGG ACAAGGGATT ATCTCAGCCA AACCTCTCCT CCAGTGTCCA AGAGGACTTG 
GGTCACCAGT ATGTGCCCAG TGAGTCAGAT TTCCCCCCAG TCTTCCACAT CAAACTCAAG 
GACCAGGTGC TGTTGGAGGG GGAAGCAGCC ACCCTGCTCT GCCTACCCGC AGCCTGCCCA 
GCACCCCGCA TCTCCTGGAT GAAAGATAAG CAGTCCCTGC GTTCAGAGCC CTCAGTGGTC 
ATTGTGTCCT GCAAAGATGG ACGGCAGCTG CTGAGCATAC CCCGGGCCGG CAAGCGGCAT 
GCTGGGCTCT ATGAGTGCTC AGCCACCAAT GTCCTGGGCA GCATTACCAG CTCCTGTACT 
GTGGCCGTGG CCCGCATTCC AGGGAAGCTA GCTCCTCCAG AGGTGCCCCA GACCTACCAT 
GACACAGCAC TAGTAGTGTG GAAGCCAGGA GACGGTCGGG CACCTTGCAC ATACACTCTG 
GAGCGGCGGG TGGATGGGGA ATCTGTCTGG CACCCTGTGA GCTCAGGCAT CCCCGACTGT 
TATTACAATG TGACTCAGCT GCCTGTCGGC GTGACCGTGC GGTTCCGTGT GGCCTGTTCC 
AATCGTGCTG GGCAGGGGCC CTTCAGCAAC CCTTCTGAGA AGGTCTTCAT CCGAGGCACT 
CCAGATTCTC CTGCTCAGCC AGCTGCTGCT CCCCGAGATG CCCCTGTTAC CTCAGGGCCA 
ACCAGGGCCC CACCGCCTGA CTCTCCTACC TCACTGGCCC CCACCCCCGC TCTTGCGCCC 
CCAGCCTCCC AGGCATCCAC TCTCAGCCCC TCAACTTCCT CCATGTCTGC CAACCAGGCC 
TTGTCCTCAC TCAAGGCTGT GGGTCCACCA CCCGCAACCC CTCCACGAAA GCACAGGGGC 
CTTCTGGCCA CCCAGCAAGC AGAGCCATCC CCACCCAGTA TCGTGGTCAC CCCAAGTGAA 
CCCAGGTCTT TTGTCCCTGA CACTGGAACC CTGACCCCAA CCTCTAGTCC TCAAGGGGTT 
AAACCAGCAC CTTCCTCAAC TTCCTTGTAT ATGGTGACTT CCTTCGTGTC TGCACCTCCA 
GCCCCCCAGG CCCCAGCCCC TGAGCCCCCT CCTGAGCCCA CCAAGGTGAC TGTGCGGAGC 
CTCAGCCCAG CCAAGGAGGT GGTCAGCTCC CCAACTCCTG AGAGCACCAC TCTTCGGCAG 
GGTCCCCCTC AGAAACCCTA CACCTTCCTG GAGGAGAAGG CCAGGGGCCG CTTTGGCGTT 
GTGCGGTCAT GCCGGGAGAA TGCTACGGGC CGAACGTTTG TCGCCAAGAT CGTGCCGTAC 
GCCGCGGAGG GCAAGCGACG CGTGCTGCAG GAATACGAGG TGCTTCGGAC GCTGCACCAC 
GAGCGGCTCA TGTCCCTGCA CGAGGCCTAC ATCACCCCTC GGTACCTCGT GCTTATCGCT 
GAGAGCTGTG GCAACCGGGA ACTCCTCTGT GGGCTCAGCG ACAGATTCCG GTATTCAGAA 
GATGACGTGG CCACCTATGT GGTGCAGCTG CTACAAGGCC TGGACTACCT CCACGGCCAC 
CACGTGCTAC ACCTGGACAT CAAGCCGGAC AATCTACTGT TGGCCGCTGA CAATGCCCTT 
AAAATTGTGG ACTTCGGCAG TGCTCAGCCC TATAACCCTC AGGCCCTGAA GCCACTTGGC 
CACCGCACAG GCACTCTGGA GTTCATGGCT CCTGAGATGG TGAAGGGAGA CCCCATTGGC 
TCTGCCACGG ACATCTGGGG AGCGGGTGTG CTCACCTACA TCATGCTTAG TGGGTACTCC 
CCATTCTATG AGCCAGACCC CCAGGAAACA GAGGCTCGGA TTGTTGGGGG TCGCTTTGAT 
GCCTTCCAGT TGTATCCTAA CACATCCCAG AGTGCCACCC TCTTCTTGAG AAAGGTCCTC 
TCAGTACATC CCTGGAGCCG GCCCTCTCTG CAGGACTGCT TGGCCCACCC ATGGCTGCAA 
GATGCCTACC TGATGAAGCT GCGCCGCCAG ACACTCACCT TCACCACCAA CCGGCTCAAG 
GAATTCCTGG GCGAGCAGCG GCGACGTCGG GCTGAGGCTG CTACCCGTCA CAAGGTGCTG 
CTCCGCTCCT ACCCTGGCAG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496466.1
CDS	900..8126
Translation	MKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_006496403.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496403.3

ORF Insert Sequence:

ATGAAGCTCA GTCCCAGCCA GGATCATGAT TCCTCCGACT CTTCTTCCAA GGCACCCCCA 
ACGTTCAAGG TCTCACTCAT GGACCAATCG GTGAGAGAAG GTCAAGATGT CATTATGAGC 
ATCCGTGTGC AGGGAGAGCC CAAGCCTGTG GTTTCCTGGC TGAGGAATCG ACAGCCCGTG 
CGCCCAGACC AGCGGCGCTT TGCAGAGGAG GCCGAGGGTG GGCTCTGCCG CTTGAGGATC 
CTGGCTGCTG AACGGGGCGA TGCTGGTTTC TACACATGCA AGGCGGTCAA CGAATATGGC 
GCTCGGCAGT GCGAGGCCCG CCTGGAGGTC CGAGCACACC CTGAAAGCCG GTCCCTGGCC 
GTGCTGGCCC CCCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC 
CTGGTGGCAG GTCCAGCTGA CGTCGAGGTG GACTGGCTGT GCCGGGGCCG CCTGCTGCAG 
CCTGCGCTGC TCAAATGCAA GATGCATTTT GATGGCCGCA AGTGCAAGCT GCTGCTCACC 
TCTGTGCACG AGGACGACAG TGGTGTCTAC ACCTGCAAGC TGAGCACAGC CAAAGATGAA 
CTGACCTGCA GTGCCCGGCT GACGGTTCGG CCGTCGCTGG CGCCTCTCTT CACCCGGTTG 
CTGGAAGATG TGGAGGTGTT GGAAGGCCGT GCTGCCCGCT TGGATTGCAA GATCAGCGGT 
ACCCCGCCTC CCTCCGTTAC CTGGACTCAT TTTGGCCACC CCGTGAATGA GGGTGACAAC 
TTGCGGCTTC AACAGGATGG AGGTCTACAC TCACTGCACA TTGCCCGGGT GGGCAGTGAG 
GACGAAGGAC TCTATGAAGT CAGTGCCACC AACACTCATG GCCAGGCCCA CTGCTCCGCC 
CAGCTGTATG TGGAGGAGCC TCGGACAGCT GCCTCTGGCC CCAGCTCGAA GCTGGAGAAG 
ATGCCATCTA TCCCTGAGGA GCCGGAGCAC GGAGATCTAG AGAGGCTGTC TATTCCTGAC 
TTCCTACGGC CACTGCAAGA TCTGGAAGTG GGACTAGCTA AGGAGGCCAT GCTGGAGTGC 
CAAGTGACCG GCCTGCCCTA TCCCACCATC AGCTGGTTCC ACAATGGCCA CCGCATTCAA 
AGCAGTGACG ACCGACGCAT GACACAGTAC AGGGACATAC ATCGCCTGGT GTTCCCAGCT 
GTGGGGCCTC AGCATGCTGG TGTCTATAAG AGTGTAATCG CCAACAAGCT GGGCAAAGCG 
GCCTGCTACG CCCATCTCTA TGTCACAGAT GTGGTTCCAG GCCCTCCAGA TGGTGCTCCG 
GAAGTAGTAG CTGTGACCGG AAGGATGGTC ACACTATCGT GGAACCCACC CAGGAGTCTG 
GACATGGCTA TTGACCCAGA TTCCCTAACA TACACTGTGC AGCACCAGGT GTTGGGCTCG 
GACCAGTGGA CGGCACTGGT CACAGGCCTG CGGGAGCCTG CGTGGGCAGC CACAGGGCTG 
AAGAAGGGGA TCCAGCACAT CTTCCGGGTC CTCAGCTCCA GTGGCAAGAG CAGCAGCAAG 
CCCTCAGCAC CGTCGGAACC CGTGCAGCTG CTAGAGCATG GCCCACCCCT GGAAGAGGCG 
CCTGCTGTGC TGGACAAGCA GGACATTGTG TATGTGGTAG AGGGCCAGCC TGCCTGTGTC 
ACCGTCACTT TTAACCATGT GGAGGCCCAG GTTGTCTGGA GGAGCTGCCG GGGAGCCCTC 
CTCGAGGCGC GCACAGGTGT GTATGAACTG AGTCAGCCAG ATGACGACCA GTATTGTCTG 
CGGATCTGCC GGGTGAGCCG ACGAGACCTG GGACCCCTTA CTTGCAGTGC CCGAAACCGC 
CACGGCACAA AGGCCTGCTC GGTCACCCTG GAGCTGGCAG AGGCACCTCG GTTTGAGTCT 
ATCATGGAAG ATGTGGAGGT GGGGCCTGGA GAAACCGCTC GCTTTGCTGT GGTAGTTGAG 
GGGAAACCAC TGCCAGACAT CATGTGGTAC AAGGATGAGG TGCTGCTGGC CGAGAGCAAC 
CATGTGAGCT TCGTGTATGA GGAGAACGAG TGTTCCCTAG TGCTGCTCAG CGCAGGAAGC 
CAGGATGGAG GCGTCTATAC CTGTACCGCC CGGAACCTGG CAGGCGAAGT CTCCTGCAAG 
GCGGAGCTGT CTGTGCTTTC AGCTCAGACA GCCATGGAGG TCGAGGGGGT CGGAGAGGAT 
GAGGAACACC GAGGAAGAAG ACTTAGCGAC TACTACGACA TCCACCAAGA GATTGGCAGG 
GGTGCCTTCT CCTACCTGCG GCGTGTAGTG GAGAGGAGCT CTGGCCTGGA GTTTGCAGCC 
AAGTTCATCC CTAGCCAGGC TAAGCCTAAA GCATCAGCAA GACGCGAGGC GCGCTTGCTG 
GCCCGGCTCC AGCATGGCTG TGTGCTCTAC TTCCACGAAG CCTTTGAGAG GCGCAGGGGA 
CTTGTCATAG TCACGGAGCT CTGCACAGAA GAGCTTCTAG AGCGCATGGC CAGGAAGCCC 
ACCGTGTGTG AGTCTGAGAC TCGGACCTAT ATGCGTCAAG TGCTAGAGGG GATATGCTAT 
CTGCATCAGA GCCATGTGCT GCACCTGGAT GTCAAGCCGG AGAACCTGCT TGTGTGGGAT 
GGTGCAGGGG GTGAAGAGCA GGTGCGCATC TGTGACTTCG GGAACGCCCA GGAACTCACT 
CCGGGAGAGC CCCAGTACTG TCAGTATGGC ACGCCAGAGT TTGTAGCACC TGAGATTGTC 
AACCAGAGCC CTGTGTCTGG AGTCACTGAC ATCTGGCCCG TGGGCGTCGT TGCCTTTCTC 
TGTTTGACGG GCATCTCCCC ATTTGTTGGG GAGAATGACC GGACAACACT CATGAATATC 
CGAAACTACA ACGTGGCATT TGAGGAGACT ACGTTCCTGA GTCTGAGCAG GGAGGCCCGG 
GGCTTCCTCA TCAAAGTGCT GGTGCAGGAT CGACTGAGAC CTACTGCAGA AGAGACATTG 
GAACATCCTT GGTTCAAAAC AGAAGCAAAG GGTGCAGAGG TGAGCACAGA TCACCTGAAG 
CTCTTCCTGT CACGGAGGAG GTGGCAGCGC TCTCAAATCA GCTACAAGTG CCACCTGGTG 
CTGCGCCCCA TTCCGGAACT GCTACGGGCT CCCCCGGAGC GGGTGTGGGT GGCCATGCCT 
AGAAGACAAC CCCCGAGTGG GGGACTCTCA TCCTCTTCAG ACTCAGAAGA GGAAGAGTTG 
GAGGAACTTC CCTCAGTACC CCGCCCCCTG CAGCCTGAGT TCTCAGGTTC TCGGGTATCC 
CTCACCGACA TCCCCACCGA AGATGAGGCT CTGGGGACCC CGGAAGCTGG GGCTGCCACC 
CCCATGGACT GGCAAGAGCA GGAAAGGACT CCCTCTAAGG ACCAGGAGGC CCCCAGCCCT 
GAGGCACTCC CCTCTCCAGG CCAGGAGTCC CCTGATGGGC CTAGCCCCAG GCGGCCAGAG 
CTCCGCAGGG GTAGCTCTGC TGAGAGTGCC CTGCCCCGAG TCGGGTCGCG GGAACCGGGC 
CGGAGCTTGC ACAAGGCAGC ATCCGTGGAG CTGCCGCAGC GCAGGAGCCC CAGCCCGGGT 
GCCACCCGCC TGACTCGGGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC 
CTGCGTCAGC GGCTGCTGAG GGGAGGCCCC GAGGATGGCA AGGTCAGCGG CCTCAGGGGA 
CCCCTGCTGG AGAGCCTGGG GGGCCGTGCA CGGGATCCCA GGATGGCAAG GGCTGCCTCC 
AGCGAGGCTG CCCCCCACCA CCAGCCCCCT CCGGAGTCCC GGGGTCTGCA AAAGAGCAGC 
AGCTTCTCTC AGGGCGAGGC AGAGCCCCGG GGTCGGCACC GCCGCGCGGG GGCACCCCTC 
GAAATCCCCG TGGCCAGACT TGGGGCTCGC AGGCTGCAGG AGTCTCCCTC CTTATCTGCC 
CTTAGCGAGA CCCAGCCACC GAGCCCTGCG AGGCCCAGCG TCCCCAAACT CAGTATCACC 
AAGTCCCCGG AACCTTCCGC TGTCACATCC AGAGACTCTC CCCAGCCCCC GGAACCTCAG 
CCTGTCCCAG AAAAGGTCCC TGAGCCCAAA CCAGAACCAG TCCGAGCTGC AAAACCAGCA 
CAACCCCCTT TGGCTCTGCA AATGCCAACG CAGCCTCTCA CCCCCTATGC CCAGATCATG 
CAGTCGCTCC AGCTGTCCAG TCCCACCCTG TCCCCACAGG ACCCCGCTGT GCCTCCATCA 
GAGCCCAAGC CCCACGCGGC TGTGTTTGCC AGGGTGGCTT CCCCACCACC CGGAGTCTCT 
GAGAAGCGCG TGCCTTCGGC CAGAACTCCC CCAGTGCTAG CCGAGAAAGC CCGAGTCCCC 
ACGGTGCCCC CCAGGCCAGG CAGCAGCCTC AGCGGCAGCA TCGAAAACCT TGAATCAGAG 
GCTGTGTTCG AGGCTAAGTT CAAACGCAGC CGCGAATCGC CACTGTCGCG GGGCCTGAGG 
CTACTGAGCC GCTCGCGCTC GGAGGAGCGC GGTCCCTTCC GTGGGGCGGA GGACGACGGC 
ATATACCGGC CCAGTCCGGC AGGAACGCCG CTAGAGCTGG TGCGACGGCC CGAGCGCTCG 
CGCTCCGTGC AGGACCTCAG GGTAGCCGGA GAGCCGGGCC TTGTGCGTCG CCTTTCGCTG 
TCCCTGTCGC AGAAGCTGCG GCGGACACCG CCGGGACAGC GACACCCTGC CTGGGAGTCC 
CGCAGCGGAG ACGGGGAGAG CTCCGAGGGC GGCAGCTCAG CTCGGGCATC CCCAGTGCTG 
GCGGTACGCA GGAGACTCAG CTCCACGCTG GAGCGTCTGT CCAGCCGTTT GCAGCGCAGC 
GGCAGCAGCG AGGACTCCGG GGGCGCCTCG GGTCGCAGCA CGCCGCTGTT TGGACGGCTG 
CGCAGGGCTA CTTCGGAAGG GGAGAGCCTG CGACGCCTCG GTGTTCCGCA CAATCAGCTG 
GGCTCTCAGA CTGGTGCCAC TACACCTTCC GCTGAGTCTC TGGGCTCAGA GGCCAGTGGC 
ACATCGGGTT CTTCAGCTCC TGGAGAAAGC CGAAGCCGGC ACCGGTGGGG CCTTTCCAGG 
CTGCGCAAGG ACAAGGGATT ATCTCAGCCA AACCTCTCCT CCAGTGTCCA AGAGGACTTG 
GGTCACCAGT ATGTGCCCAG TGAGTCAGAT TTCCCCCCAG TCTTCCACAT CAAACTCAAG 
GACCAGGTGC TGTTGGAGGG GGAAGCAGCC ACCCTGCTCT GCCTACCCGC AGCCTGCCCA 
GCACCCCGCA TCTCCTGGAT GAAAGATAAG CAGTCCCTGC GTTCAGAGCC CTCAGTGGTC 
ATTGTGTCCT GCAAAGATGG ACGGCAGCTG CTGAGCATAC CCCGGGCCGG CAAGCGGCAT 
GCTGGGCTCT ATGAGTGCTC AGCCACCAAT GTCCTGGGCA GCATTACCAG CTCCTGTACT 
GTGGCCGTGG CCCGCATTCC AGGGAAGCTA GCTCCTCCAG AGGTGCCCCA GACCTACCAT 
GACACAGCAC TAGTAGTGTG GAAGCCAGGA GACGGTCGGG CACCTTGCAC ATACACTCTG 
GAGCGGCGGG TGGATGGGGA ATCTGTCTGG CACCCTGTGA GCTCAGGCAT CCCCGACTGT 
TATTACAATG TGACTCAGCT GCCTGTCGGC GTGACCGTGC GGTTCCGTGT GGCCTGTTCC 
AATCGTGCTG GGCAGGGGCC CTTCAGCAAC CCTTCTGAGA AGGTCTTCAT CCGAGGCACT 
CCAGATTCTC CTGCTCAGCC AGCTGCTGCT CCCCGAGATG CCCCTGTTAC CTCAGGGCCA 
ACCAGGGCCC CACCGCCTGA CTCTCCTACC TCACTGGCCC CCACCCCCGC TCTTGCGCCC 
CCAGCCTCCC AGGCATCCAC TCTCAGCCCC TCAACTTCCT CCATGTCTGC CAACCAGGCC 
TTGTCCTCAC TCAAGGCTGT GGGTCCACCA CCCGCAACCC CTCCACGAAA GCACAGGGGC 
CTTCTGGCCA CCCAGCAAGC AGAGCCATCC CCACCCAGTA TCGTGGTCAC CCCAAGTGAA 
CCCAGGTCTT TTGTCCCTGA CACTGGAACC CTGACCCCAA CCTCTAGTCC TCAAGGGGTT 
AAACCAGCAC CTTCCTCAAC TTCCTTGTAT ATGGTGACTT CCTTCGTGTC TGCACCTCCA 
GCCCCCCAGG CCCCAGCCCC TGAGCCCCCT CCTGAGCCCA CCAAGGTGAC TGTGCGGAGC 
CTCAGCCCAG CCAAGGAGGT GGTCAGCTCC CCAACTCCTG AGAGCACCAC TCTTCGGCAG 
GGTCCCCCTC AGAAACCCTA CACCTTCCTG GAGGAGAAGG CCAGGGGCCG CTTTGGCGTT 
GTGCGGTCAT GCCGGGAGAA TGCTACGGGC CGAACGTTTG TCGCCAAGAT CGTGCCGTAC 
GCCGCGGAGG GCAAGCGACG CGTGCTGCAG GAATACGAGG TGCTTCGGAC GCTGCACCAC 
GAGCGGCTCA TGTCCCTGCA CGAGGCCTAC ATCACCCCTC GGTACCTCGT GCTTATCGCT 
GAGAGCTGTG GCAACCGGGA ACTCCTCTGT GGGCTCAGCG ACAGATTCCG GTATTCAGAA 
GATGACGTGG CCACCTATGT GGTGCAGCTG CTACAAGGCC TGGACTACCT CCACGGCCAC 
CACGTGCTAC ACCTGGACAT CAAGCCGGAC AATCTACTGT TGGCCGCTGA CAATGCCCTT 
AAAATTGTGG ACTTCGGCAG TGCTCAGCCC TATAACCCTC AGGCCCTGAA GCCACTTGGC 
CACCGCACAG GCACTCTGGA GTTCATGGCT CCTGAGATGG TGAAGGGAGA CCCCATTGGC 
TCTGCCACGG ACATCTGGGG AGCGGGTGTG CTCACCTACA TCATGCTTAG TGGGTACTCC 
CCATTCTATG AGCCAGACCC CCAGGAAACA GAGGCTCGGA TTGTTGGGGG TCGCTTTGAT 
GCCTTCCAGT TGTATCCTAA CACATCCCAG AGTGCCACCC TCTTCTTGAG AAAGGTCCTC 
TCAGTACATC CCTGGAGCCG GCCCTCTCTG CAGGACTGCT TGGCCCACCC ATGGCTGCAA 
GATGCCTACC TGATGAAGCT GCGCCGCCAG ACACTCACCT TCACCACCAA CCGGCTCAAG 
GAATTCCTGG GCGAGCAGCG GCGACGTCGG GCTGAGGCTG CTACCCGTCA CAAGGTGCTG 
CTCCGCTCCT ACCCTGGCAG CCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( NM_007463.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031489.4 Mus musculus SPEG complex locus (Speg), transcript variant 1, mRNA.

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CloneID	OMu00217
Clone ID Related Accession (Same CDS sequence)	NM_007463.4
Accession Version	NM_007463.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC161346.7 and AC114651.12. On Jul 25, 2007 this sequence version replaced NM_007463.3. Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1). This variant corresponds to variant SPEGbeta described in PubMed ID: 10973969. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF215896.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGACTCGG AGACTGCCGA GGATGACATC 
AGCGATGTGC CGGGGACCCA GCGCCTGGAG CTTCGGGATG ACAGGGCCTT CAGCACCCCC 
ACGGGGGGCT CTGATACCCT GGTGGGTACC TCCCTGGACA CACCCCCGAC CTCCGTGACA 
GGCACCTCAG AGGAGCAAGT GAGCTGGTGG GGCAGCGGGC AGACGGTCCT GGAGCAGGAA 
GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC CCAGGCAGCC CAAGGCAAGC ACAGACAACC 
GGGGCCGGGC CACGGCACCT GGGGGTGGAG CCGCTGGTGC GGGCATCGCG AGCTAATCTG 
GTGGGCGCAA GCTGGGGGTC AGAGGATAGC CTTTCGGTGG CCAGTGACCT CTATGGCAGC 
GCGTTCAGTC TGTACAGAGG ACGGGCGCTC TCGATCCACG TCAGCATTCC TCCGAGTGGA 
TTGCACAGAG AGGAGCCTGA CCTTCAGCCT CAGCCAGCCA GTGATGCCCT TCGTCCACGC 
CCTGCCCTTC CACCTCCCTC CAAATCCGCT TTGCTTCCCC CACCATCTCC TCGGGTGGGA 
AAGAGGGCTC TGCCAGGACC TAGTACCCAG CCCCCAGCTA CTCCCACTTC GCCCCATCGG 
CGTGCTCAGG AGCCCTCGCT GCCTGAGGAC ATCACCACCA CGGAAGAGAA GCGAGGGAAA 
AAGCCCAAGT CATCCGGGCC GTCGCTGGCG GGCACAGTGG AGTCCCGGCC CCAGACGCCA 
CTGAGCGAGG CTTCGGGTCG CCTGTCAGCA TTGGGCCGCT CGCCCCGGCT GGTGCGCGCG 
GGGTCCCGCA TCCTGGACAA GCTACAGTTC TTCGAAGAGC GGCGACGCAG CCTGGAGCGC 
AGCGACTCGC CGCCAGCGCC CCTGCGGCCC TGGGTGCCCC TGCGCAAGGC TCGCTCGCTG 
GAGCAGCCGA AGTCCGAGGG CGGTGCGGCG TGGGGCACAC CCGAGGCCTC GCAGGAGGAG 
CTGCGGTCAC CTCGGGGCAG TGTGGCAGAG CGGCGTCGCC TGTTCCAGCA AAAGGCGGCC 
TCGTTGGATG AACGCACGCG ACAACGCAGT GCAACCTCGG ACCTCGAACT CCGCTTCGCC 
CAGGAGCTGG GTCGCATCCG CCGATCTACG TCGCGGGAGG AGCTGGTGCG TTCGCACGAG 
TCCCTGCGTG CCACGCTGCA GCGCGCCCCA TCCCCTCGGG AGCCCGGCGA GCCCCCACTC 
TTCTCCCGGC CTTCCACACC CAAGACCTCA CGGGCTGTGA GCCCGGCTGC CACCCAGCCG 
CCGCCTCCTA GTGGTGCGGG CAAATCTGGG GACGAGCCTG GGAGGCCCCG AAGCAGAGGG 
CCGGTGGGCA GGACTGAACC GGGGGAAGGC CCGCAGCAGG AGATCAAGCG TCGGGACCAA 
TTCCCGCTAA CCAGGAGCAG AGCCATCCAG GAGTGCAGGA GCCCTGTGCC GCCCTACACC 
GCGGATCCCC CGGAGAGCAG GACAAAAGCC CCCTCCGGTC GCAAGCGGGA ACCCCCTGCT 
CAAGCGGTGC GCTTTCTGCC CTGGGCCACT CCGGGAGTGG AGGACTCTGT TCTGCCCCAA 
ACCTTGGAGA AGAATAGAGC GGGACCCGAG GCTGAGAAGA GGCTTCGCAG AGGACCTGAG 
GAGGATGGCC CCTGGGGGCC CTGGGACCGC AGAGGGACCC GCAGCCAAGG CAAAGGTCGC 
CGTGCTCGGC CTACTTCCCC CGAGCTCGAG TCCTCAGACG ACTCCTATGT GTCCGCTGGG 
GAAGAGCCCC TGGAGGCACC CGTGTTTGAG ATCCCTCTGC AGAATATGGT GGTGGCGCCA 
GGAGCTGACG TGCTACTTAA GTGTATCATC ACCGCCAACC CCCCACCCCA AGTGTCCTGG 
AAAAAGGATG GGTCCATGTT GCACAGCGAG GGTCGTCTTC TCATCCGGGC TGAAGGTGAA 
CGGCACACAC TGCTGCTCAG AGAGGCCCAG GCTGCCGATG CTGGGAGCTA CACAGCCACT 
GCCACCAACG AACTGGGCCA AGCTACCTGT GCTTCTTCAC TGGCTGTGAG ACCTGGCGGC 
TCCACATCCC CTTTCAGCAG CCCCATCACC TCTGATGAGG AGTACCTGAG CCCCCCAGAG 
GAGTTCCCAG AGCCTGGGGA GACCTGGCCC CGAACCCCTA CCATGAAGCT CAGTCCCAGC 
CAGGATCATG ATTCCTCCGA CTCTTCTTCC AAGGCACCCC CAACGTTCAA GGTCTCACTC 
ATGGACCAAT CGGTGAGAGA AGGTCAAGAT GTCATTATGA GCATCCGTGT GCAGGGAGAG 
CCCAAGCCTG TGGTTTCCTG GCTGAGGAAT CGACAGCCCG TGCGCCCAGA CCAGCGGCGC 
TTTGCAGAGG AGGCCGAGGG TGGGCTCTGC CGCTTGAGGA TCCTGGCTGC TGAACGGGGC 
GATGCTGGTT TCTACACATG CAAGGCGGTC AACGAATATG GCGCTCGGCA GTGCGAGGCC 
CGCCTGGAGG TCCGAGCACA CCCTGAAAGC CGGTCCCTGG CCGTGCTGGC CCCCCTGCAG 
GACGTGGACG TGGGGGCCGG GGAGATGGCG CTGTTTGAGT GCCTGGTGGC AGGTCCAGCT 
GACGTCGAGG TGGACTGGCT GTGCCGGGGC CGCCTGCTGC AGCCTGCGCT GCTCAAATGC 
AAGATGCATT TTGATGGCCG CAAGTGCAAG CTGCTGCTCA CCTCTGTGCA CGAGGACGAC 
AGTGGTGTCT ACACCTGCAA GCTGAGCACA GCCAAAGATG AACTGACCTG CAGTGCCCGG 
CTGACGGTTC GGCCGTCGCT GGCGCCTCTC TTCACCCGGT TGCTGGAAGA TGTGGAGGTG 
TTGGAAGGCC GTGCTGCCCG CTTGGATTGC AAGATCAGCG GTACCCCGCC TCCCTCCGTT 
ACCTGGACTC ATTTTGGCCA CCCCGTGAAT GAGGGTGACA ACTTGCGGCT TCAACAGGAT 
GGAGGTCTAC ACTCACTGCA CATTGCCCGG GTGGGCAGTG AGGACGAAGG ACTCTATGAA 
GTCAGTGCCA CCAACACTCA TGGCCAGGCC CACTGCTCCG CCCAGCTGTA TGTGGAGGAG 
CCTCGGACAG CTGCCTCTGG CCCCAGCTCG AAGCTGGAGA AGATGCCATC TATCCCTGAG 
GAGCCGGAGC ACGGAGATCT AGAGAGGCTG TCTATTCCTG ACTTCCTACG GCCACTGCAA 
GATCTGGAAG TGGGACTAGC TAAGGAGGCC ATGCTGGAGT GCCAAGTGAC CGGCCTGCCC 
TATCCCACCA TCAGCTGGTT CCACAATGGC CACCGCATTC AAAGCAGTGA CGACCGACGC 
ATGACACAGT ACAGGGACAT ACATCGCCTG GTGTTCCCAG CTGTGGGGCC TCAGCATGCT 
GGTGTCTATA AGAGTGTAAT CGCCAACAAG CTGGGCAAAG CGGCCTGCTA CGCCCATCTC 
TATGTCACAG ATGTGGTTCC AGGCCCTCCA GATGGTGCTC CGGAAGTAGT AGCTGTGACC 
GGAAGGATGG TCACACTATC GTGGAACCCA CCCAGGAGTC TGGACATGGC TATTGACCCA 
GATTCCCTAA CATACACTGT GCAGCACCAG GTGTTGGGCT CGGACCAGTG GACGGCACTG 
GTCACAGGCC TGCGGGAGCC TGCGTGGGCA GCCACAGGGC TGAAGAAGGG GATCCAGCAC 
ATCTTCCGGG TCCTCAGCTC CAGTGGCAAG AGCAGCAGCA AGCCCTCAGC ACCGTCGGAA 
CCCGTGCAGC TGCTAGAGCA TGGCCCACCC CTGGAAGAGG CGCCTGCTGT GCTGGACAAG 
CAGGACATTG TGTATGTGGT AGAGGGCCAG CCTGCCTGTG TCACCGTCAC TTTTAACCAT 
GTGGAGGCCC AGGTTGTCTG GAGGAGCTGC CGGGGAGCCC TCCTCGAGGC GCGCACAGGT 
GTGTATGAAC TGAGTCAGCC AGATGACGAC CAGTATTGTC TGCGGATCTG CCGGGTGAGC 
CGACGAGACC TGGGACCCCT TACTTGCAGT GCCCGAAACC GCCACGGCAC AAAGGCCTGC 
TCGGTCACCC TGGAGCTGGC AGAGGCACCT CGGTTTGAGT CTATCATGGA AGATGTGGAG 
GTGGGGCCTG GAGAAACCGC TCGCTTTGCT GTGGTAGTTG AGGGGAAACC ACTGCCAGAC 
ATCATGTGGT ACAAGGATGA GGTGCTGCTG GCCGAGAGCA ACCATGTGAG CTTCGTGTAT 
GAGGAGAACG AGTGTTCCCT AGTGCTGCTC AGCGCAGGAA GCCAGGATGG AGGCGTCTAT 
ACCTGTACCG CCCGGAACCT GGCAGGCGAA GTCTCCTGCA AGGCGGAGCT GTCTGTGCTT 
TCAGCTCAGA CAGCCATGGA GGTCGAGGGG GTCGGAGAGG ATGAGGAACA CCGAGGAAGA 
AGACTTAGCG ACTACTACGA CATCCACCAA GAGATTGGCA GGGGTGCCTT CTCCTACCTG 
CGGCGTGTAG TGGAGAGGAG CTCTGGCCTG GAGTTTGCAG CCAAGTTCAT CCCTAGCCAG 
GCTAAGCCTA AAGCATCAGC AAGACGCGAG GCGCGCTTGC TGGCCCGGCT CCAGCATGGC 
TGTGTGCTCT ACTTCCACGA AGCCTTTGAG AGGCGCAGGG GACTTGTCAT AGTCACGGAG 
CTCTGCACAG AAGAGCTTCT AGAGCGCATG GCCAGGAAGC CCACCGTGTG TGAGTCTGAG 
ACTCGGACCT ATATGCGTCA AGTGCTAGAG GGGATATGCT ATCTGCATCA GAGCCATGTG 
CTGCACCTGG ATGTCAAGCC GGAGAACCTG CTTGTGTGGG ATGGTGCAGG GGGTGAAGAG 
CAGGTGCGCA TCTGTGACTT CGGGAACGCC CAGGAACTCA CTCCGGGAGA GCCCCAGTAC 
TGTCAGTATG GCACGCCAGA GTTTGTAGCA CCTGAGATTG TCAACCAGAG CCCTGTGTCT 
GGAGTCACTG ACATCTGGCC CGTGGGCGTC GTTGCCTTTC TCTGTTTGAC GGGCATCTCC 
CCATTTGTTG GGGAGAATGA CCGGACAACA CTCATGAATA TCCGAAACTA CAACGTGGCA 
TTTGAGGAGA CTACGTTCCT GAGTCTGAGC AGGGAGGCCC GGGGCTTCCT CATCAAAGTG 
CTGGTGCAGG ATCGACTGAG ACCTACTGCA GAAGAGACAT TGGAACATCC TTGGTTCAAA 
ACAGAAGCAA AGGGTGCAGA GGTGAGCACA GATCACCTGA AGCTCTTCCT GTCACGGAGG 
AGGTGGCAGC GCTCTCAAAT CAGCTACAAG TGCCACCTGG TGCTGCGCCC CATTCCGGAA 
CTGCTACGGG CTCCCCCGGA GCGGGTGTGG GTGGCCATGC CTAGAAGACA ACCCCCGAGT 
GGGGGACTCT CATCCTCTTC AGACTCAGAA GAGGAAGAGT TGGAGGAACT TCCCTCAGTA 
CCCCGCCCCC TGCAGCCTGA GTTCTCAGGT TCTCGGGTAT CCCTCACCGA CATCCCCACC 
GAAGATGAGG CTCTGGGGAC CCCGGAAGCT GGGGCTGCCA CCCCCATGGA CTGGCAAGAG 
CAGGAAAGGA CTCCCTCTAA GGACCAGGAG GCCCCCAGCC CTGAGGCACT CCCCTCTCCA 
GGCCAGGAGT CCCCTGATGG GCCTAGCCCC AGGCGGCCAG AGCTCCGCAG GGGTAGCTCT 
GCTGAGAGTG CCCTGCCCCG AGTCGGGTCG CGGGAACCGG GCCGGAGCTT GCACAAGGCA 
GCATCCGTGG AGCTGCCGCA GCGCAGGAGC CCCAGCCCGG GTGCCACCCG CCTGACTCGG 
GGAGGCCTGG GTGAGGGCGA GTATGCCCAG AGGCTGCAGG CCCTGCGTCA GCGGCTGCTG 
AGGGGAGGCC CCGAGGATGG CAAGGTCAGC GGCCTCAGGG GACCCCTGCT GGAGAGCCTG 
GGGGGCCGTG CACGGGATCC CAGGATGGCA AGGGCTGCCT CCAGCGAGGC TGCCCCCCAC 
CACCAGCCCC CTCCGGAGTC CCGGGGTCTG CAAAAGAGCA GCAGCTTCTC TCAGGGCGAG 
GCAGAGCCCC GGGGTCGGCA CCGCCGCGCG GGGGCACCCC TCGAAATCCC CGTGGCCAGA 
CTTGGGGCTC GCAGGCTGCA GGAGTCTCCC TCCTTATCTG CCCTTAGCGA GACCCAGCCA 
CCGAGCCCTG CGAGGCCCAG CGTCCCCAAA CTCAGTATCA CCAAGTCCCC GGAACCTTCC 
GCTGTCACAT CCAGAGACTC TCCCCAGCCC CCGGAACCTC AGCCTGTCCC AGAAAAGGTC 
CCTGAGCCCA AACCAGAACC AGTCCGAGCT GCAAAACCAG CACAACCCCC TTTGGCTCTG 
CAAATGCCAA CGCAGCCTCT CACCCCCTAT GCCCAGATCA TGCAGTCGCT CCAGCTGTCC 
AGTCCCACCC TGTCCCCACA GGACCCCGCT GTGCCTCCAT CAGAGCCCAA GCCCCACGCG 
GCTGTGTTTG CCAGGGTGGC TTCCCCACCA CCCGGAGTCT CTGAGAAGCG CGTGCCTTCG 
GCCAGAACTC CCCCAGTGCT AGCCGAGAAA GCCCGAGTCC CCACGGTGCC CCCCAGGCCA 
GGCAGCAGCC TCAGCGGCAG CATCGAAAAC CTTGAATCAG AGGCTGTGTT CGAGGCTAAG 
TTCAAACGCA GCCGCGAATC GCCACTGTCG CGGGGCCTGA GGCTACTGAG CCGCTCGCGC 
TCGGAGGAGC GCGGTCCCTT CCGTGGGGCG GAGGACGACG GCATATACCG GCCCAGTCCG 
GCAGGAACGC CGCTAGAGCT GGTGCGACGG CCCGAGCGCT CGCGCTCCGT GCAGGACCTC 
AGGGTAGCCG GAGAGCCGGG CCTTGTGCGT CGCCTTTCGC TGTCCCTGTC GCAGAAGCTG 
CGGCGGACAC CGCCGGGACA GCGACACCCT GCCTGGGAGT CCCGCAGCGG AGACGGGGAG 
AGCTCCGAGG GCGGCAGCTC AGCTCGGGCA TCCCCAGTGC TGGCGGTACG CAGGAGACTC 
AGCTCCACGC TGGAGCGTCT GTCCAGCCGT TTGCAGCGCA GCGGCAGCAG CGAGGACTCC 
GGGGGCGCCT CGGGTCGCAG CACGCCGCTG TTTGGACGGC TGCGCAGGGC TACTTCGGAA 
GGGGAGAGCC TGCGACGCCT CGGTGTTCCG CACAATCAGC TGGGCTCTCA GACTGGTGCC 
ACTACACCTT CCGCTGAGTC TCTGGGCTCA GAGGCCAGTG GCACATCGGG TTCTTCAGCT 
CCTGGAGAAA GCCGAAGCCG GCACCGGTGG GGCCTTTCCA GGCTGCGCAA GGACAAGGGA 
TTATCTCAGC CAAACCTCTC CTCCAGTGTC CAAGAGGACT TGGGTCACCA GTATGTGCCC 
AGTGAGTCAG ATTTCCCCCC AGTCTTCCAC ATCAAACTCA AGGACCAGGT GCTGTTGGAG 
GGGGAAGCAG CCACCCTGCT CTGCCTACCC GCAGCCTGCC CAGCACCCCG CATCTCCTGG 
ATGAAAGATA AGCAGTCCCT GCGTTCAGAG CCCTCAGTGG TCATTGTGTC CTGCAAAGAT 
GGACGGCAGC TGCTGAGCAT ACCCCGGGCC GGCAAGCGGC ATGCTGGGCT CTATGAGTGC 
TCAGCCACCA ATGTCCTGGG CAGCATTACC AGCTCCTGTA CTGTGGCCGT GGCCCGCATT 
CCAGGGAAGC TAGCTCCTCC AGAGGTGCCC CAGACCTACC ATGACACAGC ACTAGTAGTG 
TGGAAGCCAG GAGACGGTCG GGCACCTTGC ACATACACTC TGGAGCGGCG GGTGGATGGG 
GAATCTGTCT GGCACCCTGT GAGCTCAGGC ATCCCCGACT GTTATTACAA TGTGACTCAG 
CTGCCTGTCG GCGTGACCGT GCGGTTCCGT GTGGCCTGTT CCAATCGTGC TGGGCAGGGG 
CCCTTCAGCA ACCCTTCTGA GAAGGTCTTC ATCCGAGGCA CTCCAGATTC TCCTGCTCAG 
CCAGCTGCTG CTCCCCGAGA TGCCCCTGTT ACCTCAGGGC CAACCAGGGC CCCACCGCCT 
GACTCTCCTA CCTCACTGGC CCCCACCCCC GCTCTTGCGC CCCCAGCCTC CCAGGCATCC 
ACTCTCAGCC CCTCAACTTC CTCCATGTCT GCCAACCAGG CCTTGTCCTC ACTCAAGGCT 
GTGGGTCCAC CACCCGCAAC CCCTCCACGA AAGCACAGGG GCCTTCTGGC CACCCAGCAA 
GCAGAGCCAT CCCCACCCAG TATCGTGGTC ACCCCAAGTG AACCCAGGTC TTTTGTCCCT 
GACACTGGAA CCCTGACCCC AACCTCTAGT CCTCAAGGGG TTAAACCAGC ACCTTCCTCA 
ACTTCCTTGT ATATGGTGAC TTCCTTCGTG TCTGCACCTC CAGCCCCCCA GGCCCCAGCC 
CCTGAGCCCC CTCCTGAGCC CACCAAGGTG ACTGTGCGGA GCCTCAGCCC AGCCAAGGAG 
GTGGTCAGCT CCCCAACTCC TGAGAGCACC ACTCTTCGGC AGGGTCCCCC TCAGAAACCC 
TACACCTTCC TGGAGGAGAA GGCCAGGGGC CGCTTTGGCG TTGTGCGGTC ATGCCGGGAG 
AATGCTACGG GCCGAACGTT TGTCGCCAAG ATCGTGCCGT ACGCCGCGGA GGGCAAGCGA 
CGCGTGCTGC AGGAATACGA GGTGCTTCGG ACGCTGCACC ACGAGCGGCT CATGTCCCTG 
CACGAGGCCT ACATCACCCC TCGGTACCTC GTGCTTATCG CTGAGAGCTG TGGCAACCGG 
GAACTCCTCT GTGGGCTCAG CGACAGATTC CGGTATTCAG AAGATGACGT GGCCACCTAT 
GTGGTGCAGC TGCTACAAGG CCTGGACTAC CTCCACGGCC ACCACGTGCT ACACCTGGAC 
ATCAAGCCGG ACAATCTACT GTTGGCCGCT GACAATGCCC TTAAAATTGT GGACTTCGGC 
AGTGCTCAGC CCTATAACCC TCAGGCCCTG AAGCCACTTG GCCACCGCAC AGGCACTCTG 
GAGTTCATGG CTCCTGAGAT GGTGAAGGGA GACCCCATTG GCTCTGCCAC GGACATCTGG 
GGAGCGGGTG TGCTCACCTA CATCATGCTT AGTGGGTACT CCCCATTCTA TGAGCCAGAC 
CCCCAGGAAA CAGAGGCTCG GATTGTTGGG GGTCGCTTTG ATGCCTTCCA GTTGTATCCT 
AACACATCCC AGAGTGCCAC CCTCTTCTTG AGAAAGGTCC TCTCAGTACA TCCCTGGAGC 
CGGCCCTCTC TGCAGGACTG CTTGGCCCAC CCATGGCTGC AAGATGCCTA CCTGATGAAG 
CTGCGCCGCC AGACACTCAC CTTCACCACC AACCGGCTCA AGGAATTCCT GGGCGAGCAG 
CGGCGACGTC GGGCTGAGGC TGCTACCCGT CACAAGGTGC TGCTCCGCTC CTACCCTGGC 
AGCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031489.4
CDS	144..9932
Translation	MQKARGTRGEDAGTRAPPSPGVPPKRAKVGAGRGVLVTGDGAGAPVFLRPLKNAAVCAGSDVRLRVVVSGTPQPSLSWFRDGQLLPPPAPEPSCLWLRSCGAQDAGVYSCSAQNERGQASCEAVLTVLEVRDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPRQAQTTGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	NM_007463.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007463.4

ORF Insert Sequence:

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGACTCGG AGACTGCCGA GGATGACATC 
AGCGATGTGC CGGGGACCCA GCGCCTGGAG CTTCGGGATG ACAGGGCCTT CAGCACCCCC 
ACGGGGGGCT CTGATACCCT GGTGGGTACC TCCCTGGACA CACCCCCGAC CTCCGTGACA 
GGCACCTCAG AGGAGCAAGT GAGCTGGTGG GGCAGCGGGC AGACGGTCCT GGAGCAGGAA 
GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC CCAGGCAGCC CAAGGCAAGC ACAGACAACC 
GGGGCCGGGC CACGGCACCT GGGGGTGGAG CCGCTGGTGC GGGCATCGCG AGCTAATCTG 
GTGGGCGCAA GCTGGGGGTC AGAGGATAGC CTTTCGGTGG CCAGTGACCT CTATGGCAGC 
GCGTTCAGTC TGTACAGAGG ACGGGCGCTC TCGATCCACG TCAGCATTCC TCCGAGTGGA 
TTGCACAGAG AGGAGCCTGA CCTTCAGCCT CAGCCAGCCA GTGATGCCCT TCGTCCACGC 
CCTGCCCTTC CACCTCCCTC CAAATCCGCT TTGCTTCCCC CACCATCTCC TCGGGTGGGA 
AAGAGGGCTC TGCCAGGACC TAGTACCCAG CCCCCAGCTA CTCCCACTTC GCCCCATCGG 
CGTGCTCAGG AGCCCTCGCT GCCTGAGGAC ATCACCACCA CGGAAGAGAA GCGAGGGAAA 
AAGCCCAAGT CATCCGGGCC GTCGCTGGCG GGCACAGTGG AGTCCCGGCC CCAGACGCCA 
CTGAGCGAGG CTTCGGGTCG CCTGTCAGCA TTGGGCCGCT CGCCCCGGCT GGTGCGCGCG 
GGGTCCCGCA TCCTGGACAA GCTACAGTTC TTCGAAGAGC GGCGACGCAG CCTGGAGCGC 
AGCGACTCGC CGCCAGCGCC CCTGCGGCCC TGGGTGCCCC TGCGCAAGGC TCGCTCGCTG 
GAGCAGCCGA AGTCCGAGGG CGGTGCGGCG TGGGGCACAC CCGAGGCCTC GCAGGAGGAG 
CTGCGGTCAC CTCGGGGCAG TGTGGCAGAG CGGCGTCGCC TGTTCCAGCA AAAGGCGGCC 
TCGTTGGATG AACGCACGCG ACAACGCAGT GCAACCTCGG ACCTCGAACT CCGCTTCGCC 
CAGGAGCTGG GTCGCATCCG CCGATCTACG TCGCGGGAGG AGCTGGTGCG TTCGCACGAG 
TCCCTGCGTG CCACGCTGCA GCGCGCCCCA TCCCCTCGGG AGCCCGGCGA GCCCCCACTC 
TTCTCCCGGC CTTCCACACC CAAGACCTCA CGGGCTGTGA GCCCGGCTGC CACCCAGCCG 
CCGCCTCCTA GTGGTGCGGG CAAATCTGGG GACGAGCCTG GGAGGCCCCG AAGCAGAGGG 
CCGGTGGGCA GGACTGAACC GGGGGAAGGC CCGCAGCAGG AGATCAAGCG TCGGGACCAA 
TTCCCGCTAA CCAGGAGCAG AGCCATCCAG GAGTGCAGGA GCCCTGTGCC GCCCTACACC 
GCGGATCCCC CGGAGAGCAG GACAAAAGCC CCCTCCGGTC GCAAGCGGGA ACCCCCTGCT 
CAAGCGGTGC GCTTTCTGCC CTGGGCCACT CCGGGAGTGG AGGACTCTGT TCTGCCCCAA 
ACCTTGGAGA AGAATAGAGC GGGACCCGAG GCTGAGAAGA GGCTTCGCAG AGGACCTGAG 
GAGGATGGCC CCTGGGGGCC CTGGGACCGC AGAGGGACCC GCAGCCAAGG CAAAGGTCGC 
CGTGCTCGGC CTACTTCCCC CGAGCTCGAG TCCTCAGACG ACTCCTATGT GTCCGCTGGG 
GAAGAGCCCC TGGAGGCACC CGTGTTTGAG ATCCCTCTGC AGAATATGGT GGTGGCGCCA 
GGAGCTGACG TGCTACTTAA GTGTATCATC ACCGCCAACC CCCCACCCCA AGTGTCCTGG 
AAAAAGGATG GGTCCATGTT GCACAGCGAG GGTCGTCTTC TCATCCGGGC TGAAGGTGAA 
CGGCACACAC TGCTGCTCAG AGAGGCCCAG GCTGCCGATG CTGGGAGCTA CACAGCCACT 
GCCACCAACG AACTGGGCCA AGCTACCTGT GCTTCTTCAC TGGCTGTGAG ACCTGGCGGC 
TCCACATCCC CTTTCAGCAG CCCCATCACC TCTGATGAGG AGTACCTGAG CCCCCCAGAG 
GAGTTCCCAG AGCCTGGGGA GACCTGGCCC CGAACCCCTA CCATGAAGCT CAGTCCCAGC 
CAGGATCATG ATTCCTCCGA CTCTTCTTCC AAGGCACCCC CAACGTTCAA GGTCTCACTC 
ATGGACCAAT CGGTGAGAGA AGGTCAAGAT GTCATTATGA GCATCCGTGT GCAGGGAGAG 
CCCAAGCCTG TGGTTTCCTG GCTGAGGAAT CGACAGCCCG TGCGCCCAGA CCAGCGGCGC 
TTTGCAGAGG AGGCCGAGGG TGGGCTCTGC CGCTTGAGGA TCCTGGCTGC TGAACGGGGC 
GATGCTGGTT TCTACACATG CAAGGCGGTC AACGAATATG GCGCTCGGCA GTGCGAGGCC 
CGCCTGGAGG TCCGAGCACA CCCTGAAAGC CGGTCCCTGG CCGTGCTGGC CCCCCTGCAG 
GACGTGGACG TGGGGGCCGG GGAGATGGCG CTGTTTGAGT GCCTGGTGGC AGGTCCAGCT 
GACGTCGAGG TGGACTGGCT GTGCCGGGGC CGCCTGCTGC AGCCTGCGCT GCTCAAATGC 
AAGATGCATT TTGATGGCCG CAAGTGCAAG CTGCTGCTCA CCTCTGTGCA CGAGGACGAC 
AGTGGTGTCT ACACCTGCAA GCTGAGCACA GCCAAAGATG AACTGACCTG CAGTGCCCGG 
CTGACGGTTC GGCCGTCGCT GGCGCCTCTC TTCACCCGGT TGCTGGAAGA TGTGGAGGTG 
TTGGAAGGCC GTGCTGCCCG CTTGGATTGC AAGATCAGCG GTACCCCGCC TCCCTCCGTT 
ACCTGGACTC ATTTTGGCCA CCCCGTGAAT GAGGGTGACA ACTTGCGGCT TCAACAGGAT 
GGAGGTCTAC ACTCACTGCA CATTGCCCGG GTGGGCAGTG AGGACGAAGG ACTCTATGAA 
GTCAGTGCCA CCAACACTCA TGGCCAGGCC CACTGCTCCG CCCAGCTGTA TGTGGAGGAG 
CCTCGGACAG CTGCCTCTGG CCCCAGCTCG AAGCTGGAGA AGATGCCATC TATCCCTGAG 
GAGCCGGAGC ACGGAGATCT AGAGAGGCTG TCTATTCCTG ACTTCCTACG GCCACTGCAA 
GATCTGGAAG TGGGACTAGC TAAGGAGGCC ATGCTGGAGT GCCAAGTGAC CGGCCTGCCC 
TATCCCACCA TCAGCTGGTT CCACAATGGC CACCGCATTC AAAGCAGTGA CGACCGACGC 
ATGACACAGT ACAGGGACAT ACATCGCCTG GTGTTCCCAG CTGTGGGGCC TCAGCATGCT 
GGTGTCTATA AGAGTGTAAT CGCCAACAAG CTGGGCAAAG CGGCCTGCTA CGCCCATCTC 
TATGTCACAG ATGTGGTTCC AGGCCCTCCA GATGGTGCTC CGGAAGTAGT AGCTGTGACC 
GGAAGGATGG TCACACTATC GTGGAACCCA CCCAGGAGTC TGGACATGGC TATTGACCCA 
GATTCCCTAA CATACACTGT GCAGCACCAG GTGTTGGGCT CGGACCAGTG GACGGCACTG 
GTCACAGGCC TGCGGGAGCC TGCGTGGGCA GCCACAGGGC TGAAGAAGGG GATCCAGCAC 
ATCTTCCGGG TCCTCAGCTC CAGTGGCAAG AGCAGCAGCA AGCCCTCAGC ACCGTCGGAA 
CCCGTGCAGC TGCTAGAGCA TGGCCCACCC CTGGAAGAGG CGCCTGCTGT GCTGGACAAG 
CAGGACATTG TGTATGTGGT AGAGGGCCAG CCTGCCTGTG TCACCGTCAC TTTTAACCAT 
GTGGAGGCCC AGGTTGTCTG GAGGAGCTGC CGGGGAGCCC TCCTCGAGGC GCGCACAGGT 
GTGTATGAAC TGAGTCAGCC AGATGACGAC CAGTATTGTC TGCGGATCTG CCGGGTGAGC 
CGACGAGACC TGGGACCCCT TACTTGCAGT GCCCGAAACC GCCACGGCAC AAAGGCCTGC 
TCGGTCACCC TGGAGCTGGC AGAGGCACCT CGGTTTGAGT CTATCATGGA AGATGTGGAG 
GTGGGGCCTG GAGAAACCGC TCGCTTTGCT GTGGTAGTTG AGGGGAAACC ACTGCCAGAC 
ATCATGTGGT ACAAGGATGA GGTGCTGCTG GCCGAGAGCA ACCATGTGAG CTTCGTGTAT 
GAGGAGAACG AGTGTTCCCT AGTGCTGCTC AGCGCAGGAA GCCAGGATGG AGGCGTCTAT 
ACCTGTACCG CCCGGAACCT GGCAGGCGAA GTCTCCTGCA AGGCGGAGCT GTCTGTGCTT 
TCAGCTCAGA CAGCCATGGA GGTCGAGGGG GTCGGAGAGG ATGAGGAACA CCGAGGAAGA 
AGACTTAGCG ACTACTACGA CATCCACCAA GAGATTGGCA GGGGTGCCTT CTCCTACCTG 
CGGCGTGTAG TGGAGAGGAG CTCTGGCCTG GAGTTTGCAG CCAAGTTCAT CCCTAGCCAG 
GCTAAGCCTA AAGCATCAGC AAGACGCGAG GCGCGCTTGC TGGCCCGGCT CCAGCATGGC 
TGTGTGCTCT ACTTCCACGA AGCCTTTGAG AGGCGCAGGG GACTTGTCAT AGTCACGGAG 
CTCTGCACAG AAGAGCTTCT AGAGCGCATG GCCAGGAAGC CCACCGTGTG TGAGTCTGAG 
ACTCGGACCT ATATGCGTCA AGTGCTAGAG GGGATATGCT ATCTGCATCA GAGCCATGTG 
CTGCACCTGG ATGTCAAGCC GGAGAACCTG CTTGTGTGGG ATGGTGCAGG GGGTGAAGAG 
CAGGTGCGCA TCTGTGACTT CGGGAACGCC CAGGAACTCA CTCCGGGAGA GCCCCAGTAC 
TGTCAGTATG GCACGCCAGA GTTTGTAGCA CCTGAGATTG TCAACCAGAG CCCTGTGTCT 
GGAGTCACTG ACATCTGGCC CGTGGGCGTC GTTGCCTTTC TCTGTTTGAC GGGCATCTCC 
CCATTTGTTG GGGAGAATGA CCGGACAACA CTCATGAATA TCCGAAACTA CAACGTGGCA 
TTTGAGGAGA CTACGTTCCT GAGTCTGAGC AGGGAGGCCC GGGGCTTCCT CATCAAAGTG 
CTGGTGCAGG ATCGACTGAG ACCTACTGCA GAAGAGACAT TGGAACATCC TTGGTTCAAA 
ACAGAAGCAA AGGGTGCAGA GGTGAGCACA GATCACCTGA AGCTCTTCCT GTCACGGAGG 
AGGTGGCAGC GCTCTCAAAT CAGCTACAAG TGCCACCTGG TGCTGCGCCC CATTCCGGAA 
CTGCTACGGG CTCCCCCGGA GCGGGTGTGG GTGGCCATGC CTAGAAGACA ACCCCCGAGT 
GGGGGACTCT CATCCTCTTC AGACTCAGAA GAGGAAGAGT TGGAGGAACT TCCCTCAGTA 
CCCCGCCCCC TGCAGCCTGA GTTCTCAGGT TCTCGGGTAT CCCTCACCGA CATCCCCACC 
GAAGATGAGG CTCTGGGGAC CCCGGAAGCT GGGGCTGCCA CCCCCATGGA CTGGCAAGAG 
CAGGAAAGGA CTCCCTCTAA GGACCAGGAG GCCCCCAGCC CTGAGGCACT CCCCTCTCCA 
GGCCAGGAGT CCCCTGATGG GCCTAGCCCC AGGCGGCCAG AGCTCCGCAG GGGTAGCTCT 
GCTGAGAGTG CCCTGCCCCG AGTCGGGTCG CGGGAACCGG GCCGGAGCTT GCACAAGGCA 
GCATCCGTGG AGCTGCCGCA GCGCAGGAGC CCCAGCCCGG GTGCCACCCG CCTGACTCGG 
GGAGGCCTGG GTGAGGGCGA GTATGCCCAG AGGCTGCAGG CCCTGCGTCA GCGGCTGCTG 
AGGGGAGGCC CCGAGGATGG CAAGGTCAGC GGCCTCAGGG GACCCCTGCT GGAGAGCCTG 
GGGGGCCGTG CACGGGATCC CAGGATGGCA AGGGCTGCCT CCAGCGAGGC TGCCCCCCAC 
CACCAGCCCC CTCCGGAGTC CCGGGGTCTG CAAAAGAGCA GCAGCTTCTC TCAGGGCGAG 
GCAGAGCCCC GGGGTCGGCA CCGCCGCGCG GGGGCACCCC TCGAAATCCC CGTGGCCAGA 
CTTGGGGCTC GCAGGCTGCA GGAGTCTCCC TCCTTATCTG CCCTTAGCGA GACCCAGCCA 
CCGAGCCCTG CGAGGCCCAG CGTCCCCAAA CTCAGTATCA CCAAGTCCCC GGAACCTTCC 
GCTGTCACAT CCAGAGACTC TCCCCAGCCC CCGGAACCTC AGCCTGTCCC AGAAAAGGTC 
CCTGAGCCCA AACCAGAACC AGTCCGAGCT GCAAAACCAG CACAACCCCC TTTGGCTCTG 
CAAATGCCAA CGCAGCCTCT CACCCCCTAT GCCCAGATCA TGCAGTCGCT CCAGCTGTCC 
AGTCCCACCC TGTCCCCACA GGACCCCGCT GTGCCTCCAT CAGAGCCCAA GCCCCACGCG 
GCTGTGTTTG CCAGGGTGGC TTCCCCACCA CCCGGAGTCT CTGAGAAGCG CGTGCCTTCG 
GCCAGAACTC CCCCAGTGCT AGCCGAGAAA GCCCGAGTCC CCACGGTGCC CCCCAGGCCA 
GGCAGCAGCC TCAGCGGCAG CATCGAAAAC CTTGAATCAG AGGCTGTGTT CGAGGCTAAG 
TTCAAACGCA GCCGCGAATC GCCACTGTCG CGGGGCCTGA GGCTACTGAG CCGCTCGCGC 
TCGGAGGAGC GCGGTCCCTT CCGTGGGGCG GAGGACGACG GCATATACCG GCCCAGTCCG 
GCAGGAACGC CGCTAGAGCT GGTGCGACGG CCCGAGCGCT CGCGCTCCGT GCAGGACCTC 
AGGGTAGCCG GAGAGCCGGG CCTTGTGCGT CGCCTTTCGC TGTCCCTGTC GCAGAAGCTG 
CGGCGGACAC CGCCGGGACA GCGACACCCT GCCTGGGAGT CCCGCAGCGG AGACGGGGAG 
AGCTCCGAGG GCGGCAGCTC AGCTCGGGCA TCCCCAGTGC TGGCGGTACG CAGGAGACTC 
AGCTCCACGC TGGAGCGTCT GTCCAGCCGT TTGCAGCGCA GCGGCAGCAG CGAGGACTCC 
GGGGGCGCCT CGGGTCGCAG CACGCCGCTG TTTGGACGGC TGCGCAGGGC TACTTCGGAA 
GGGGAGAGCC TGCGACGCCT CGGTGTTCCG CACAATCAGC TGGGCTCTCA GACTGGTGCC 
ACTACACCTT CCGCTGAGTC TCTGGGCTCA GAGGCCAGTG GCACATCGGG TTCTTCAGCT 
CCTGGAGAAA GCCGAAGCCG GCACCGGTGG GGCCTTTCCA GGCTGCGCAA GGACAAGGGA 
TTATCTCAGC CAAACCTCTC CTCCAGTGTC CAAGAGGACT TGGGTCACCA GTATGTGCCC 
AGTGAGTCAG ATTTCCCCCC AGTCTTCCAC ATCAAACTCA AGGACCAGGT GCTGTTGGAG 
GGGGAAGCAG CCACCCTGCT CTGCCTACCC GCAGCCTGCC CAGCACCCCG CATCTCCTGG 
ATGAAAGATA AGCAGTCCCT GCGTTCAGAG CCCTCAGTGG TCATTGTGTC CTGCAAAGAT 
GGACGGCAGC TGCTGAGCAT ACCCCGGGCC GGCAAGCGGC ATGCTGGGCT CTATGAGTGC 
TCAGCCACCA ATGTCCTGGG CAGCATTACC AGCTCCTGTA CTGTGGCCGT GGCCCGCATT 
CCAGGGAAGC TAGCTCCTCC AGAGGTGCCC CAGACCTACC ATGACACAGC ACTAGTAGTG 
TGGAAGCCAG GAGACGGTCG GGCACCTTGC ACATACACTC TGGAGCGGCG GGTGGATGGG 
GAATCTGTCT GGCACCCTGT GAGCTCAGGC ATCCCCGACT GTTATTACAA TGTGACTCAG 
CTGCCTGTCG GCGTGACCGT GCGGTTCCGT GTGGCCTGTT CCAATCGTGC TGGGCAGGGG 
CCCTTCAGCA ACCCTTCTGA GAAGGTCTTC ATCCGAGGCA CTCCAGATTC TCCTGCTCAG 
CCAGCTGCTG CTCCCCGAGA TGCCCCTGTT ACCTCAGGGC CAACCAGGGC CCCACCGCCT 
GACTCTCCTA CCTCACTGGC CCCCACCCCC GCTCTTGCGC CCCCAGCCTC CCAGGCATCC 
ACTCTCAGCC CCTCAACTTC CTCCATGTCT GCCAACCAGG CCTTGTCCTC ACTCAAGGCT 
GTGGGTCCAC CACCCGCAAC CCCTCCACGA AAGCACAGGG GCCTTCTGGC CACCCAGCAA 
GCAGAGCCAT CCCCACCCAG TATCGTGGTC ACCCCAAGTG AACCCAGGTC TTTTGTCCCT 
GACACTGGAA CCCTGACCCC AACCTCTAGT CCTCAAGGGG TTAAACCAGC ACCTTCCTCA 
ACTTCCTTGT ATATGGTGAC TTCCTTCGTG TCTGCACCTC CAGCCCCCCA GGCCCCAGCC 
CCTGAGCCCC CTCCTGAGCC CACCAAGGTG ACTGTGCGGA GCCTCAGCCC AGCCAAGGAG 
GTGGTCAGCT CCCCAACTCC TGAGAGCACC ACTCTTCGGC AGGGTCCCCC TCAGAAACCC 
TACACCTTCC TGGAGGAGAA GGCCAGGGGC CGCTTTGGCG TTGTGCGGTC ATGCCGGGAG 
AATGCTACGG GCCGAACGTT TGTCGCCAAG ATCGTGCCGT ACGCCGCGGA GGGCAAGCGA 
CGCGTGCTGC AGGAATACGA GGTGCTTCGG ACGCTGCACC ACGAGCGGCT CATGTCCCTG 
CACGAGGCCT ACATCACCCC TCGGTACCTC GTGCTTATCG CTGAGAGCTG TGGCAACCGG 
GAACTCCTCT GTGGGCTCAG CGACAGATTC CGGTATTCAG AAGATGACGT GGCCACCTAT 
GTGGTGCAGC TGCTACAAGG CCTGGACTAC CTCCACGGCC ACCACGTGCT ACACCTGGAC 
ATCAAGCCGG ACAATCTACT GTTGGCCGCT GACAATGCCC TTAAAATTGT GGACTTCGGC 
AGTGCTCAGC CCTATAACCC TCAGGCCCTG AAGCCACTTG GCCACCGCAC AGGCACTCTG 
GAGTTCATGG CTCCTGAGAT GGTGAAGGGA GACCCCATTG GCTCTGCCAC GGACATCTGG 
GGAGCGGGTG TGCTCACCTA CATCATGCTT AGTGGGTACT CCCCATTCTA TGAGCCAGAC 
CCCCAGGAAA CAGAGGCTCG GATTGTTGGG GGTCGCTTTG ATGCCTTCCA GTTGTATCCT 
AACACATCCC AGAGTGCCAC CCTCTTCTTG AGAAAGGTCC TCTCAGTACA TCCCTGGAGC 
CGGCCCTCTC TGCAGGACTG CTTGGCCCAC CCATGGCTGC AAGATGCCTA CCTGATGAAG 
CTGCGCCGCC AGACACTCAC CTTCACCACC AACCGGCTCA AGGAATTCCT GGGCGAGCAG 
CGGCGACGTC GGGCTGAGGC TGCTACCCGT CACAAGGTGC TGCTCCGCTC CTACCCTGGC 
AGCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_011238643.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236945.1 Mus musculus SPEG complex locus (Speg), transcript variant X1, mRNA.

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CloneID	OMu67144
Clone ID Related Accession (Same CDS sequence)	XM_011238643.1 , XM_011238643.2
Accession Version	XM_011238643.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
TACGACTCGG AGACTGCCGA GGATGACATC AGCGATGTGC CGGGGACCCA GCGCCTGGAG 
CTTCGGGATG ACAGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGTACC 
TCCCTGGACA CACCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG 
GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC 
CCAGGCAGCC CAAGGCAAGC ACAGACAACC GGGGCCGGGC CACGGCACCT GGGGGTGGAG 
CCGCTGGTGC GGGCATCGCG AGCTAATCTG GTGGGCGCAA GCTGGGGGTC AGAGGATAGC 
CTTTCGGTGG CCAGTGACCT CTATGGCAGC GCGTTCAGTC TGTACAGAGG ACGGGCGCTC 
TCGATCCACG TCAGCATTCC TCCGAGTGGA TTGCACAGAG AGGAGCCTGA CCTTCAGCCT 
CAGCCAGCCA GTGATGCCCT TCGTCCACGC CCTGCCCTTC CACCTCCCTC CAAATCCGCT 
TTGCTTCCCC CACCATCTCC TCGGGTGGGA AAGAGGGCTC TGCCAGGACC TAGTACCCAG 
CCCCCAGCTA CTCCCACTTC GCCCCATCGG CGTGCTCAGG AGCCCTCGCT GCCTGAGGAC 
ATCACCACCA CGGAAGAGAA GCGAGGGAAA AAGCCCAAGT CATCCGGGCC GTCGCTGGCG 
GGCACAGTGG AGTCCCGGCC CCAGACGCCA CTGAGCGAGG CTTCGGGTCG CCTGTCAGCA 
TTGGGCCGCT CGCCCCGGCT GGTGCGCGCG GGGTCCCGCA TCCTGGACAA GCTACAGTTC 
TTCGAAGAGC GGCGACGCAG CCTGGAGCGC AGCGACTCGC CGCCAGCGCC CCTGCGGCCC 
TGGGTGCCCC TGCGCAAGGC TCGCTCGCTG GAGCAGCCGA AGTCCGAGGG CGGTGCGGCG 
TGGGGCACAC CCGAGGCCTC GCAGGAGGAG CTGCGGTCAC CTCGGGGCAG TGTGGCAGAG 
CGGCGTCGCC TGTTCCAGCA AAAGGCGGCC TCGTTGGATG AACGCACGCG ACAACGCAGT 
GCAACCTCGG ACCTCGAACT CCGCTTCGCC CAGGAGCTGG GTCGCATCCG CCGATCTACG 
TCGCGGGAGG AGCTGGTGCG TTCGCACGAG TCCCTGCGTG CCACGCTGCA GCGCGCCCCA 
TCCCCTCGGG AGCCCGGCGA GCCCCCACTC TTCTCCCGGC CTTCCACACC CAAGACCTCA 
CGGGCTGTGA GCCCGGCTGC CACCCAGCCG CCGCCTCCTA GTGGTGCGGG CAAATCTGGG 
GACGAGCCTG GGAGGCCCCG AAGCAGAGGG CCGGTGGGCA GGACTGAACC GGGGGAAGGC 
CCGCAGCAGG AGATCAAGCG TCGGGACCAA TTCCCGCTAA CCAGGAGCAG AGCCATCCAG 
GAGTGCAGGA GCCCTGTGCC GCCCTACACC GCGGATCCCC CGGAGAGCAG GACAAAAGCC 
CCCTCCGGTC GCAAGCGGGA ACCCCCTGCT CAAGCGGTGC GCTTTCTGCC CTGGGCCACT 
CCGGGAGTGG AGGACTCTGT TCTGCCCCAA ACCTTGGAGA AGAATAGAGC GGGACCCGAG 
GCTGAGAAGA GGCTTCGCAG AGGACCTGAG GAGGATGGCC CCTGGGGGCC CTGGGACCGC 
AGAGGGACCC GCAGCCAAGG CAAAGGTCGC CGTGCTCGGC CTACTTCCCC CGAGCTCGAG 
TCCTCAGACG ACTCCTATGT GTCCGCTGGG GAAGAGCCCC TGGAGGCACC CGTGTTTGAG 
ATCCCTCTGC AGAATATGGT GGTGGCGCCA GGAGCTGACG TGCTACTTAA GTGTATCATC 
ACCGCCAACC CCCCACCCCA AGTGTCCTGG AAAAAGGATG GGTCCATGTT GCACAGCGAG 
GGTCGTCTTC TCATCCGGGC TGAAGGTGAA CGGCACACAC TGCTGCTCAG AGAGGCCCAG 
GCTGCCGATG CTGGGAGCTA CACAGCCACT GCCACCAACG AACTGGGCCA AGCTACCTGT 
GCTTCTTCAC TGGCTGTGAG ACCTGGCGGC TCCACATCCC CTTTCAGCAG CCCCATCACC 
TCTGATGAGG AGTACCTGAG CCCCCCAGAG GAGTTCCCAG AGCCTGGGGA GACCTGGCCC 
CGAACCCCTA CCATGAAGCT CAGTCCCAGC CAGGATCATG ATTCCTCCGA CTCTTCTTCC 
AAGGCACCCC CAACGTTCAA GGTCTCACTC ATGGACCAAT CGGTGAGAGA AGGTCAAGAT 
GTCATTATGA GCATCCGTGT GCAGGGAGAG CCCAAGCCTG TGGTTTCCTG GCTGAGGAAT 
CGACAGCCCG TGCGCCCAGA CCAGCGGCGC TTTGCAGAGG AGGCCGAGGG TGGGCTCTGC 
CGCTTGAGGA TCCTGGCTGC TGAACGGGGC GATGCTGGTT TCTACACATG CAAGGCGGTC 
AACGAATATG GCGCTCGGCA GTGCGAGGCC CGCCTGGAGG TCCGAGCACA CCCTGAAAGC 
CGGTCCCTGG CCGTGCTGGC CCCCCTGCAG GACGTGGACG TGGGGGCCGG GGAGATGGCG 
CTGTTTGAGT GCCTGGTGGC AGGTCCAGCT GACGTCGAGG TGGACTGGCT GTGCCGGGGC 
CGCCTGCTGC AGCCTGCGCT GCTCAAATGC AAGATGCATT TTGATGGCCG CAAGTGCAAG 
CTGCTGCTCA CCTCTGTGCA CGAGGACGAC AGTGGTGTCT ACACCTGCAA GCTGAGCACA 
GCCAAAGATG AACTGACCTG CAGTGCCCGG CTGACGGTTC GGCCGTCGCT GGCGCCTCTC 
TTCACCCGGT TGCTGGAAGA TGTGGAGGTG TTGGAAGGCC GTGCTGCCCG CTTGGATTGC 
AAGATCAGCG GTACCCCGCC TCCCTCCGTT ACCTGGACTC ATTTTGGCCA CCCCGTGAAT 
GAGGGTGACA ACTTGCGGCT TCAACAGGAT GGAGGTCTAC ACTCACTGCA CATTGCCCGG 
GTGGGCAGTG AGGACGAAGG ACTCTATGAA GTCAGTGCCA CCAACACTCA TGGCCAGGCC 
CACTGCTCCG CCCAGCTGTA TGTGGAGGAG CCTCGGACAG CTGCCTCTGG CCCCAGCTCG 
AAGCTGGAGA AGATGCCATC TATCCCTGAG GAGCCGGAGC ACGGAGATCT AGAGAGGCTG 
TCTATTCCTG ACTTCCTACG GCCACTGCAA GATCTGGAAG TGGGACTAGC TAAGGAGGCC 
ATGCTGGAGT GCCAAGTGAC CGGCCTGCCC TATCCCACCA TCAGCTGGTT CCACAATGGC 
CACCGCATTC AAAGCAGTGA CGACCGACGC ATGACACAGT ACAGGGACAT ACATCGCCTG 
GTGTTCCCAG CTGTGGGGCC TCAGCATGCT GGTGTCTATA AGAGTGTAAT CGCCAACAAG 
CTGGGCAAAG CGGCCTGCTA CGCCCATCTC TATGTCACAG ATGTGGTTCC AGGCCCTCCA 
GATGGTGCTC CGGAAGTAGT AGCTGTGACC GGAAGGATGG TCACACTATC GTGGAACCCA 
CCCAGGAGTC TGGACATGGC TATTGACCCA GATTCCCTAA CATACACTGT GCAGCACCAG 
GTGTTGGGCT CGGACCAGTG GACGGCACTG GTCACAGGCC TGCGGGAGCC TGCGTGGGCA 
GCCACAGGGC TGAAGAAGGG GATCCAGCAC ATCTTCCGGG TCCTCAGCTC CAGTGGCAAG 
AGCAGCAGCA AGCCCTCAGC ACCGTCGGAA CCCGTGCAGC TGCTAGAGCA TGGCCCACCC 
CTGGAAGAGG CGCCTGCTGT GCTGGACAAG CAGGACATTG TGTATGTGGT AGAGGGCCAG 
CCTGCCTGTG TCACCGTCAC TTTTAACCAT GTGGAGGCCC AGGTTGTCTG GAGGAGCTGC 
CGGGGAGCCC TCCTCGAGGC GCGCACAGGT GTGTATGAAC TGAGTCAGCC AGATGACGAC 
CAGTATTGTC TGCGGATCTG CCGGGTGAGC CGACGAGACC TGGGACCCCT TACTTGCAGT 
GCCCGAAACC GCCACGGCAC AAAGGCCTGC TCGGTCACCC TGGAGCTGGC AGAGGCACCT 
CGGTTTGAGT CTATCATGGA AGATGTGGAG GTGGGGCCTG GAGAAACCGC TCGCTTTGCT 
GTGGTAGTTG AGGGGAAACC ACTGCCAGAC ATCATGTGGT ACAAGGATGA GGTGCTGCTG 
GCCGAGAGCA ACCATGTGAG CTTCGTGTAT GAGGAGAACG AGTGTTCCCT AGTGCTGCTC 
AGCGCAGGAA GCCAGGATGG AGGCGTCTAT ACCTGTACCG CCCGGAACCT GGCAGGCGAA 
GTCTCCTGCA AGGCGGAGCT GTCTGTGCTT TCAGCTCAGA CAGCCATGGA GGTCGAGGGG 
GTCGGAGAGG ATGAGGAACA CCGAGGAAGA AGACTTAGCG ACTACTACGA CATCCACCAA 
GAGATTGGCA GGGGTGCCTT CTCCTACCTG CGGCGTGTAG TGGAGAGGAG CTCTGGCCTG 
GAGTTTGCAG CCAAGTTCAT CCCTAGCCAG GCTAAGCCTA AAGCATCAGC AAGACGCGAG 
GCGCGCTTGC TGGCCCGGCT CCAGCATGGC TGTGTGCTCT ACTTCCACGA AGCCTTTGAG 
AGGCGCAGGG GACTTGTCAT AGTCACGGAG CTCTGCACAG AAGAGCTTCT AGAGCGCATG 
GCCAGGAAGC CCACCGTGTG TGAGTCTGAG ACTCGGACCT ATATGCGTCA AGTGCTAGAG 
GGGATATGCT ATCTGCATCA GAGCCATGTG CTGCACCTGG ATGTCAAGCC GGAGAACCTG 
CTTGTGTGGG ATGGTGCAGG GGGTGAAGAG CAGGTGCGCA TCTGTGACTT CGGGAACGCC 
CAGGAACTCA CTCCGGGAGA GCCCCAGTAC TGTCAGTATG GCACGCCAGA GTTTGTAGCA 
CCTGAGATTG TCAACCAGAG CCCTGTGTCT GGAGTCACTG ACATCTGGCC CGTGGGCGTC 
GTTGCCTTTC TCTGTTTGAC GGGCATCTCC CCATTTGTTG GGGAGAATGA CCGGACAACA 
CTCATGAATA TCCGAAACTA CAACGTGGCA TTTGAGGAGA CTACGTTCCT GAGTCTGAGC 
AGGGAGGCCC GGGGCTTCCT CATCAAAGTG CTGGTGCAGG ATCGACTGAG ACCTACTGCA 
GAAGAGACAT TGGAACATCC TTGGTTCAAA ACAGAAGCAA AGGGTGCAGA GGTGAGCACA 
GATCACCTGA AGCTCTTCCT GTCACGGAGG AGGTGGCAGC GCTCTCAAAT CAGCTACAAG 
TGCCACCTGG TGCTGCGCCC CATTCCGGAA CTGCTACGGG CTCCCCCGGA GCGGGTGTGG 
GTGGCCATGC CTAGAAGACA ACCCCCGAGT GGGGGACTCT CATCCTCTTC AGACTCAGAA 
GAGGAAGAGT TGGAGGAACT TCCCTCAGTA CCCCGCCCCC TGCAGCCTGA GTTCTCAGGT 
TCTCGGGTAT CCCTCACCGA CATCCCCACC GAAGATGAGG CTCTGGGGAC CCCGGAAGCT 
GGGGCTGCCA CCCCCATGGA CTGGCAAGAG CAGGAAAGGA CTCCCTCTAA GGACCAGGAG 
GCCCCCAGCC CTGAGGCACT CCCCTCTCCA GGCCAGGAGT CCCCTGATGG GCCTAGCCCC 
AGGCGGCCAG AGCTCCGCAG GGGTAGCTCT GCTGAGAGTG CCCTGCCCCG AGTCGGGTCG 
CGGGAACCGG GCCGGAGCTT GCACAAGGCA GCATCCGTGG AGCTGCCGCA GCGCAGGAGC 
CCCAGCCCGG GTGCCACCCG CCTGACTCGG GGAGGCCTGG GTGAGGGCGA GTATGCCCAG 
AGGCTGCAGG CCCTGCGTCA GCGGCTGCTG AGGGGAGGCC CCGAGGATGG CAAGGTCAGC 
GGCCTCAGGG GACCCCTGCT GGAGAGCCTG GGGGGCCGTG CACGGGATCC CAGGATGGCA 
AGGGCTGCCT CCAGCGAGGC TGCCCCCCAC CACCAGCCCC CTCCGGAGTC CCGGGGTCTG 
CAAAAGAGCA GCAGCTTCTC TCAGGGCGAG GCAGAGCCCC GGGGTCGGCA CCGCCGCGCG 
GGGGCACCCC TCGAAATCCC CGTGGCCAGA CTTGGGGCTC GCAGGCTGCA GGAGTCTCCC 
TCCTTATCTG CCCTTAGCGA GACCCAGCCA CCGAGCCCTG CGAGGCCCAG CGTCCCCAAA 
CTCAGTATCA CCAAGTCCCC GGAACCTTCC GCTGTCACAT CCAGAGACTC TCCCCAGCCC 
CCGGAACCTC AGCCTGTCCC AGAAAAGGTC CCTGAGCCCA AACCAGAACC AGTCCGAGCT 
GCAAAACCAG CACAACCCCC TTTGGCTCTG CAAATGCCAA CGCAGCCTCT CACCCCCTAT 
GCCCAGATCA TGCAGTCGCT CCAGCTGTCC AGTCCCACCC TGTCCCCACA GGACCCCGCT 
GTGCCTCCAT CAGAGCCCAA GCCCCACGCG GCTGTGTTTG CCAGGGTGGC TTCCCCACCA 
CCCGGAGTCT CTGAGAAGCG CGTGCCTTCG GCCAGAACTC CCCCAGTGCT AGCCGAGAAA 
GCCCGAGTCC CCACGGTGCC CCCCAGGCCA GGCAGCAGCC TCAGCGGCAG CATCGAAAAC 
CTTGAATCAG AGGCTGTGTT CGAGGCTAAG TTCAAACGCA GCCGCGAATC GCCACTGTCG 
CGGGGCCTGA GGCTACTGAG CCGCTCGCGC TCGGAGGAGC GCGGTCCCTT CCGTGGGGCG 
GAGGACGACG GCATATACCG GCCCAGTCCG GCAGGAACGC CGCTAGAGCT GGTGCGACGG 
CCCGAGCGCT CGCGCTCCGT GCAGGACCTC AGGGTAGCCG GAGAGCCGGG CCTTGTGCGT 
CGCCTTTCGC TGTCCCTGTC GCAGAAGCTG CGGCGGACAC CGCCGGGACA GCGACACCCT 
GCCTGGGAGT CCCGCAGCGG AGACGGGGAG AGCTCCGAGG GCGGCAGCTC AGCTCGGGCA 
TCCCCAGTGC TGGCGGTACG CAGGAGACTC AGCTCCACGC TGGAGCGTCT GTCCAGCCGT 
TTGCAGCGCA GCGGCAGCAG CGAGGACTCC GGGGGCGCCT CGGGTCGCAG CACGCCGCTG 
TTTGGACGGC TGCGCAGGGC TACTTCGGAA GGGGAGAGCC TGCGACGCCT CGGTGTTCCG 
CACAATCAGC TGGGCTCTCA GACTGGTGCC ACTACACCTT CCGCTGAGTC TCTGGGCTCA 
GAGGCCAGTG GCACATCGGG TTCTTCAGCT CCTGGAGAAA GCCGAAGCCG GCACCGGTGG 
GGCCTTTCCA GGCTGCGCAA GGACAAGGGA TTATCTCAGC CAAACCTCTC CTCCAGTGTC 
CAAGAGGACT TGGGTCACCA GTATGTGCCC AGTGAGTCAG ATTTCCCCCC AGTCTTCCAC 
ATCAAACTCA AGGACCAGGT GCTGTTGGAG GGGGAAGCAG CCACCCTGCT CTGCCTACCC 
GCAGCCTGCC CAGCACCCCG CATCTCCTGG ATGAAAGATA AGCAGTCCCT GCGTTCAGAG 
CCCTCAGTGG TCATTGTGTC CTGCAAAGAT GGACGGCAGC TGCTGAGCAT ACCCCGGGCC 
GGCAAGCGGC ATGCTGGGCT CTATGAGTGC TCAGCCACCA ATGTCCTGGG CAGCATTACC 
AGCTCCTGTA CTGTGGCCGT GGCCCGCATT CCAGGGAAGC TAGCTCCTCC AGAGGTGCCC 
CAGACCTACC ATGACACAGC ACTAGTAGTG TGGAAGCCAG GAGACGGTCG GGCACCTTGC 
ACATACACTC TGGAGCGGCG GGTGGATGGG GAATCTGTCT GGCACCCTGT GAGCTCAGGC 
ATCCCCGACT GTTATTACAA TGTGACTCAG CTGCCTGTCG GCGTGACCGT GCGGTTCCGT 
GTGGCCTGTT CCAATCGTGC TGGGCAGGGG CCCTTCAGCA ACCCTTCTGA GAAGGTCTTC 
ATCCGAGGCA CTCCAGATTC TCCTGCTCAG CCAGCTGCTG CTCCCCGAGA TGCCCCTGTT 
ACCTCAGGGC CAACCAGGGC CCCACCGCCT GACTCTCCTA CCTCACTGGC CCCCACCCCC 
GCTCTTGCGC CCCCAGCCTC CCAGGCATCC ACTCTCAGCC CCTCAACTTC CTCCATGTCT 
GCCAACCAGG CCTTGTCCTC ACTCAAGGCT GTGGGTCCAC CACCCGCAAC CCCTCCACGA 
AAGCACAGGG GCCTTCTGGC CACCCAGCAA GCAGAGCCAT CCCCACCCAG TATCGTGGTC 
ACCCCAAGTG AACCCAGGTC TTTTGTCCCT GACACTGGAA CCCTGACCCC AACCTCTAGT 
CCTCAAGGGG TTAAACCAGC ACCTTCCTCA ACTTCCTTGT ATATGGTGAC TTCCTTCGTG 
TCTGCACCTC CAGCCCCCCA GGCCCCAGCC CCTGAGCCCC CTCCTGAGCC CACCAAGGTG 
ACTGTGCGGA GCCTCAGCCC AGCCAAGGAG GTGGTCAGCT CCCCAACTCC TGAGAGCACC 
ACTCTTCGGC AGGGTCCCCC TCAGAAACCC TACACCTTCC TGGAGGAGAA GGCCAGGGGC 
CGCTTTGGCG TTGTGCGGTC ATGCCGGGAG AATGCTACGG GCCGAACGTT TGTCGCCAAG 
ATCGTGCCGT ACGCCGCGGA GGGCAAGCGA CGCGTGCTGC AGGAATACGA GGTGCTTCGG 
ACGCTGCACC ACGAGCGGCT CATGTCCCTG CACGAGGCCT ACATCACCCC TCGGTACCTC 
GTGCTTATCG CTGAGAGCTG TGGCAACCGG GAACTCCTCT GTGGGCTCAG CGACAGATTC 
CGGTATTCAG AAGATGACGT GGCCACCTAT GTGGTGCAGC TGCTACAAGG CCTGGACTAC 
CTCCACGGCC ACCACGTGCT ACACCTGGAC ATCAAGCCGG ACAATCTACT GTTGGCCGCT 
GACAATGCCC TTAAAATTGT GGACTTCGGC AGTGCTCAGC CCTATAACCC TCAGGCCCTG 
AAGCCACTTG GCCACCGCAC AGGCACTCTG GAGTTCATGG CTCCTGAGAT GGTGAAGGGA 
GACCCCATTG GCTCTGCCAC GGACATCTGG GGAGCGGGTG TGCTCACCTA CATCATGCTT 
AGTGGGTACT CCCCATTCTA TGAGCCAGAC CCCCAGGAAA CAGAGGCTCG GATTGTTGGG 
GGTCGCTTTG ATGCCTTCCA GTTGTATCCT AACACATCCC AGAGTGCCAC CCTCTTCTTG 
AGAAAGGTCC TCTCAGTACA TCCCTGGAGC CGGCCCTCTC TGCAGGACTG CTTGGCCCAC 
CCATGGCTGC AAGATGCCTA CCTGATGAAG CTGCGCCGCC AGACACTCAC CTTCACCACC 
AACCGGCTCA AGGAATTCCT GGGCGAGCAG CGGCGACGTC GGGCTGAGGC TGCTACCCGT 
CACAAGGTGC TGCTCCGCTC CTACCCTGGC AGCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236945.1
CDS	107..9925
Translation	MQKARGTRGEDAGTRAPPSPGVPPKRAKVGAGRGVLVTGDGAGAPVFLRPLKNAAVCAGSDVRLRVVVSGTPQPSLSWFRDGQLLPPPAPEPSCLWLRSCGAQDAGVYSCSAQNERGQASCEAVLTVLEVRVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPRQAQTTGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_011238643.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238643.1

ORF Insert Sequence:

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
TACGACTCGG AGACTGCCGA GGATGACATC AGCGATGTGC CGGGGACCCA GCGCCTGGAG 
CTTCGGGATG ACAGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGTACC 
TCCCTGGACA CACCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG 
GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC 
CCAGGCAGCC CAAGGCAAGC ACAGACAACC GGGGCCGGGC CACGGCACCT GGGGGTGGAG 
CCGCTGGTGC GGGCATCGCG AGCTAATCTG GTGGGCGCAA GCTGGGGGTC AGAGGATAGC 
CTTTCGGTGG CCAGTGACCT CTATGGCAGC GCGTTCAGTC TGTACAGAGG ACGGGCGCTC 
TCGATCCACG TCAGCATTCC TCCGAGTGGA TTGCACAGAG AGGAGCCTGA CCTTCAGCCT 
CAGCCAGCCA GTGATGCCCT TCGTCCACGC CCTGCCCTTC CACCTCCCTC CAAATCCGCT 
TTGCTTCCCC CACCATCTCC TCGGGTGGGA AAGAGGGCTC TGCCAGGACC TAGTACCCAG 
CCCCCAGCTA CTCCCACTTC GCCCCATCGG CGTGCTCAGG AGCCCTCGCT GCCTGAGGAC 
ATCACCACCA CGGAAGAGAA GCGAGGGAAA AAGCCCAAGT CATCCGGGCC GTCGCTGGCG 
GGCACAGTGG AGTCCCGGCC CCAGACGCCA CTGAGCGAGG CTTCGGGTCG CCTGTCAGCA 
TTGGGCCGCT CGCCCCGGCT GGTGCGCGCG GGGTCCCGCA TCCTGGACAA GCTACAGTTC 
TTCGAAGAGC GGCGACGCAG CCTGGAGCGC AGCGACTCGC CGCCAGCGCC CCTGCGGCCC 
TGGGTGCCCC TGCGCAAGGC TCGCTCGCTG GAGCAGCCGA AGTCCGAGGG CGGTGCGGCG 
TGGGGCACAC CCGAGGCCTC GCAGGAGGAG CTGCGGTCAC CTCGGGGCAG TGTGGCAGAG 
CGGCGTCGCC TGTTCCAGCA AAAGGCGGCC TCGTTGGATG AACGCACGCG ACAACGCAGT 
GCAACCTCGG ACCTCGAACT CCGCTTCGCC CAGGAGCTGG GTCGCATCCG CCGATCTACG 
TCGCGGGAGG AGCTGGTGCG TTCGCACGAG TCCCTGCGTG CCACGCTGCA GCGCGCCCCA 
TCCCCTCGGG AGCCCGGCGA GCCCCCACTC TTCTCCCGGC CTTCCACACC CAAGACCTCA 
CGGGCTGTGA GCCCGGCTGC CACCCAGCCG CCGCCTCCTA GTGGTGCGGG CAAATCTGGG 
GACGAGCCTG GGAGGCCCCG AAGCAGAGGG CCGGTGGGCA GGACTGAACC GGGGGAAGGC 
CCGCAGCAGG AGATCAAGCG TCGGGACCAA TTCCCGCTAA CCAGGAGCAG AGCCATCCAG 
GAGTGCAGGA GCCCTGTGCC GCCCTACACC GCGGATCCCC CGGAGAGCAG GACAAAAGCC 
CCCTCCGGTC GCAAGCGGGA ACCCCCTGCT CAAGCGGTGC GCTTTCTGCC CTGGGCCACT 
CCGGGAGTGG AGGACTCTGT TCTGCCCCAA ACCTTGGAGA AGAATAGAGC GGGACCCGAG 
GCTGAGAAGA GGCTTCGCAG AGGACCTGAG GAGGATGGCC CCTGGGGGCC CTGGGACCGC 
AGAGGGACCC GCAGCCAAGG CAAAGGTCGC CGTGCTCGGC CTACTTCCCC CGAGCTCGAG 
TCCTCAGACG ACTCCTATGT GTCCGCTGGG GAAGAGCCCC TGGAGGCACC CGTGTTTGAG 
ATCCCTCTGC AGAATATGGT GGTGGCGCCA GGAGCTGACG TGCTACTTAA GTGTATCATC 
ACCGCCAACC CCCCACCCCA AGTGTCCTGG AAAAAGGATG GGTCCATGTT GCACAGCGAG 
GGTCGTCTTC TCATCCGGGC TGAAGGTGAA CGGCACACAC TGCTGCTCAG AGAGGCCCAG 
GCTGCCGATG CTGGGAGCTA CACAGCCACT GCCACCAACG AACTGGGCCA AGCTACCTGT 
GCTTCTTCAC TGGCTGTGAG ACCTGGCGGC TCCACATCCC CTTTCAGCAG CCCCATCACC 
TCTGATGAGG AGTACCTGAG CCCCCCAGAG GAGTTCCCAG AGCCTGGGGA GACCTGGCCC 
CGAACCCCTA CCATGAAGCT CAGTCCCAGC CAGGATCATG ATTCCTCCGA CTCTTCTTCC 
AAGGCACCCC CAACGTTCAA GGTCTCACTC ATGGACCAAT CGGTGAGAGA AGGTCAAGAT 
GTCATTATGA GCATCCGTGT GCAGGGAGAG CCCAAGCCTG TGGTTTCCTG GCTGAGGAAT 
CGACAGCCCG TGCGCCCAGA CCAGCGGCGC TTTGCAGAGG AGGCCGAGGG TGGGCTCTGC 
CGCTTGAGGA TCCTGGCTGC TGAACGGGGC GATGCTGGTT TCTACACATG CAAGGCGGTC 
AACGAATATG GCGCTCGGCA GTGCGAGGCC CGCCTGGAGG TCCGAGCACA CCCTGAAAGC 
CGGTCCCTGG CCGTGCTGGC CCCCCTGCAG GACGTGGACG TGGGGGCCGG GGAGATGGCG 
CTGTTTGAGT GCCTGGTGGC AGGTCCAGCT GACGTCGAGG TGGACTGGCT GTGCCGGGGC 
CGCCTGCTGC AGCCTGCGCT GCTCAAATGC AAGATGCATT TTGATGGCCG CAAGTGCAAG 
CTGCTGCTCA CCTCTGTGCA CGAGGACGAC AGTGGTGTCT ACACCTGCAA GCTGAGCACA 
GCCAAAGATG AACTGACCTG CAGTGCCCGG CTGACGGTTC GGCCGTCGCT GGCGCCTCTC 
TTCACCCGGT TGCTGGAAGA TGTGGAGGTG TTGGAAGGCC GTGCTGCCCG CTTGGATTGC 
AAGATCAGCG GTACCCCGCC TCCCTCCGTT ACCTGGACTC ATTTTGGCCA CCCCGTGAAT 
GAGGGTGACA ACTTGCGGCT TCAACAGGAT GGAGGTCTAC ACTCACTGCA CATTGCCCGG 
GTGGGCAGTG AGGACGAAGG ACTCTATGAA GTCAGTGCCA CCAACACTCA TGGCCAGGCC 
CACTGCTCCG CCCAGCTGTA TGTGGAGGAG CCTCGGACAG CTGCCTCTGG CCCCAGCTCG 
AAGCTGGAGA AGATGCCATC TATCCCTGAG GAGCCGGAGC ACGGAGATCT AGAGAGGCTG 
TCTATTCCTG ACTTCCTACG GCCACTGCAA GATCTGGAAG TGGGACTAGC TAAGGAGGCC 
ATGCTGGAGT GCCAAGTGAC CGGCCTGCCC TATCCCACCA TCAGCTGGTT CCACAATGGC 
CACCGCATTC AAAGCAGTGA CGACCGACGC ATGACACAGT ACAGGGACAT ACATCGCCTG 
GTGTTCCCAG CTGTGGGGCC TCAGCATGCT GGTGTCTATA AGAGTGTAAT CGCCAACAAG 
CTGGGCAAAG CGGCCTGCTA CGCCCATCTC TATGTCACAG ATGTGGTTCC AGGCCCTCCA 
GATGGTGCTC CGGAAGTAGT AGCTGTGACC GGAAGGATGG TCACACTATC GTGGAACCCA 
CCCAGGAGTC TGGACATGGC TATTGACCCA GATTCCCTAA CATACACTGT GCAGCACCAG 
GTGTTGGGCT CGGACCAGTG GACGGCACTG GTCACAGGCC TGCGGGAGCC TGCGTGGGCA 
GCCACAGGGC TGAAGAAGGG GATCCAGCAC ATCTTCCGGG TCCTCAGCTC CAGTGGCAAG 
AGCAGCAGCA AGCCCTCAGC ACCGTCGGAA CCCGTGCAGC TGCTAGAGCA TGGCCCACCC 
CTGGAAGAGG CGCCTGCTGT GCTGGACAAG CAGGACATTG TGTATGTGGT AGAGGGCCAG 
CCTGCCTGTG TCACCGTCAC TTTTAACCAT GTGGAGGCCC AGGTTGTCTG GAGGAGCTGC 
CGGGGAGCCC TCCTCGAGGC GCGCACAGGT GTGTATGAAC TGAGTCAGCC AGATGACGAC 
CAGTATTGTC TGCGGATCTG CCGGGTGAGC CGACGAGACC TGGGACCCCT TACTTGCAGT 
GCCCGAAACC GCCACGGCAC AAAGGCCTGC TCGGTCACCC TGGAGCTGGC AGAGGCACCT 
CGGTTTGAGT CTATCATGGA AGATGTGGAG GTGGGGCCTG GAGAAACCGC TCGCTTTGCT 
GTGGTAGTTG AGGGGAAACC ACTGCCAGAC ATCATGTGGT ACAAGGATGA GGTGCTGCTG 
GCCGAGAGCA ACCATGTGAG CTTCGTGTAT GAGGAGAACG AGTGTTCCCT AGTGCTGCTC 
AGCGCAGGAA GCCAGGATGG AGGCGTCTAT ACCTGTACCG CCCGGAACCT GGCAGGCGAA 
GTCTCCTGCA AGGCGGAGCT GTCTGTGCTT TCAGCTCAGA CAGCCATGGA GGTCGAGGGG 
GTCGGAGAGG ATGAGGAACA CCGAGGAAGA AGACTTAGCG ACTACTACGA CATCCACCAA 
GAGATTGGCA GGGGTGCCTT CTCCTACCTG CGGCGTGTAG TGGAGAGGAG CTCTGGCCTG 
GAGTTTGCAG CCAAGTTCAT CCCTAGCCAG GCTAAGCCTA AAGCATCAGC AAGACGCGAG 
GCGCGCTTGC TGGCCCGGCT CCAGCATGGC TGTGTGCTCT ACTTCCACGA AGCCTTTGAG 
AGGCGCAGGG GACTTGTCAT AGTCACGGAG CTCTGCACAG AAGAGCTTCT AGAGCGCATG 
GCCAGGAAGC CCACCGTGTG TGAGTCTGAG ACTCGGACCT ATATGCGTCA AGTGCTAGAG 
GGGATATGCT ATCTGCATCA GAGCCATGTG CTGCACCTGG ATGTCAAGCC GGAGAACCTG 
CTTGTGTGGG ATGGTGCAGG GGGTGAAGAG CAGGTGCGCA TCTGTGACTT CGGGAACGCC 
CAGGAACTCA CTCCGGGAGA GCCCCAGTAC TGTCAGTATG GCACGCCAGA GTTTGTAGCA 
CCTGAGATTG TCAACCAGAG CCCTGTGTCT GGAGTCACTG ACATCTGGCC CGTGGGCGTC 
GTTGCCTTTC TCTGTTTGAC GGGCATCTCC CCATTTGTTG GGGAGAATGA CCGGACAACA 
CTCATGAATA TCCGAAACTA CAACGTGGCA TTTGAGGAGA CTACGTTCCT GAGTCTGAGC 
AGGGAGGCCC GGGGCTTCCT CATCAAAGTG CTGGTGCAGG ATCGACTGAG ACCTACTGCA 
GAAGAGACAT TGGAACATCC TTGGTTCAAA ACAGAAGCAA AGGGTGCAGA GGTGAGCACA 
GATCACCTGA AGCTCTTCCT GTCACGGAGG AGGTGGCAGC GCTCTCAAAT CAGCTACAAG 
TGCCACCTGG TGCTGCGCCC CATTCCGGAA CTGCTACGGG CTCCCCCGGA GCGGGTGTGG 
GTGGCCATGC CTAGAAGACA ACCCCCGAGT GGGGGACTCT CATCCTCTTC AGACTCAGAA 
GAGGAAGAGT TGGAGGAACT TCCCTCAGTA CCCCGCCCCC TGCAGCCTGA GTTCTCAGGT 
TCTCGGGTAT CCCTCACCGA CATCCCCACC GAAGATGAGG CTCTGGGGAC CCCGGAAGCT 
GGGGCTGCCA CCCCCATGGA CTGGCAAGAG CAGGAAAGGA CTCCCTCTAA GGACCAGGAG 
GCCCCCAGCC CTGAGGCACT CCCCTCTCCA GGCCAGGAGT CCCCTGATGG GCCTAGCCCC 
AGGCGGCCAG AGCTCCGCAG GGGTAGCTCT GCTGAGAGTG CCCTGCCCCG AGTCGGGTCG 
CGGGAACCGG GCCGGAGCTT GCACAAGGCA GCATCCGTGG AGCTGCCGCA GCGCAGGAGC 
CCCAGCCCGG GTGCCACCCG CCTGACTCGG GGAGGCCTGG GTGAGGGCGA GTATGCCCAG 
AGGCTGCAGG CCCTGCGTCA GCGGCTGCTG AGGGGAGGCC CCGAGGATGG CAAGGTCAGC 
GGCCTCAGGG GACCCCTGCT GGAGAGCCTG GGGGGCCGTG CACGGGATCC CAGGATGGCA 
AGGGCTGCCT CCAGCGAGGC TGCCCCCCAC CACCAGCCCC CTCCGGAGTC CCGGGGTCTG 
CAAAAGAGCA GCAGCTTCTC TCAGGGCGAG GCAGAGCCCC GGGGTCGGCA CCGCCGCGCG 
GGGGCACCCC TCGAAATCCC CGTGGCCAGA CTTGGGGCTC GCAGGCTGCA GGAGTCTCCC 
TCCTTATCTG CCCTTAGCGA GACCCAGCCA CCGAGCCCTG CGAGGCCCAG CGTCCCCAAA 
CTCAGTATCA CCAAGTCCCC GGAACCTTCC GCTGTCACAT CCAGAGACTC TCCCCAGCCC 
CCGGAACCTC AGCCTGTCCC AGAAAAGGTC CCTGAGCCCA AACCAGAACC AGTCCGAGCT 
GCAAAACCAG CACAACCCCC TTTGGCTCTG CAAATGCCAA CGCAGCCTCT CACCCCCTAT 
GCCCAGATCA TGCAGTCGCT CCAGCTGTCC AGTCCCACCC TGTCCCCACA GGACCCCGCT 
GTGCCTCCAT CAGAGCCCAA GCCCCACGCG GCTGTGTTTG CCAGGGTGGC TTCCCCACCA 
CCCGGAGTCT CTGAGAAGCG CGTGCCTTCG GCCAGAACTC CCCCAGTGCT AGCCGAGAAA 
GCCCGAGTCC CCACGGTGCC CCCCAGGCCA GGCAGCAGCC TCAGCGGCAG CATCGAAAAC 
CTTGAATCAG AGGCTGTGTT CGAGGCTAAG TTCAAACGCA GCCGCGAATC GCCACTGTCG 
CGGGGCCTGA GGCTACTGAG CCGCTCGCGC TCGGAGGAGC GCGGTCCCTT CCGTGGGGCG 
GAGGACGACG GCATATACCG GCCCAGTCCG GCAGGAACGC CGCTAGAGCT GGTGCGACGG 
CCCGAGCGCT CGCGCTCCGT GCAGGACCTC AGGGTAGCCG GAGAGCCGGG CCTTGTGCGT 
CGCCTTTCGC TGTCCCTGTC GCAGAAGCTG CGGCGGACAC CGCCGGGACA GCGACACCCT 
GCCTGGGAGT CCCGCAGCGG AGACGGGGAG AGCTCCGAGG GCGGCAGCTC AGCTCGGGCA 
TCCCCAGTGC TGGCGGTACG CAGGAGACTC AGCTCCACGC TGGAGCGTCT GTCCAGCCGT 
TTGCAGCGCA GCGGCAGCAG CGAGGACTCC GGGGGCGCCT CGGGTCGCAG CACGCCGCTG 
TTTGGACGGC TGCGCAGGGC TACTTCGGAA GGGGAGAGCC TGCGACGCCT CGGTGTTCCG 
CACAATCAGC TGGGCTCTCA GACTGGTGCC ACTACACCTT CCGCTGAGTC TCTGGGCTCA 
GAGGCCAGTG GCACATCGGG TTCTTCAGCT CCTGGAGAAA GCCGAAGCCG GCACCGGTGG 
GGCCTTTCCA GGCTGCGCAA GGACAAGGGA TTATCTCAGC CAAACCTCTC CTCCAGTGTC 
CAAGAGGACT TGGGTCACCA GTATGTGCCC AGTGAGTCAG ATTTCCCCCC AGTCTTCCAC 
ATCAAACTCA AGGACCAGGT GCTGTTGGAG GGGGAAGCAG CCACCCTGCT CTGCCTACCC 
GCAGCCTGCC CAGCACCCCG CATCTCCTGG ATGAAAGATA AGCAGTCCCT GCGTTCAGAG 
CCCTCAGTGG TCATTGTGTC CTGCAAAGAT GGACGGCAGC TGCTGAGCAT ACCCCGGGCC 
GGCAAGCGGC ATGCTGGGCT CTATGAGTGC TCAGCCACCA ATGTCCTGGG CAGCATTACC 
AGCTCCTGTA CTGTGGCCGT GGCCCGCATT CCAGGGAAGC TAGCTCCTCC AGAGGTGCCC 
CAGACCTACC ATGACACAGC ACTAGTAGTG TGGAAGCCAG GAGACGGTCG GGCACCTTGC 
ACATACACTC TGGAGCGGCG GGTGGATGGG GAATCTGTCT GGCACCCTGT GAGCTCAGGC 
ATCCCCGACT GTTATTACAA TGTGACTCAG CTGCCTGTCG GCGTGACCGT GCGGTTCCGT 
GTGGCCTGTT CCAATCGTGC TGGGCAGGGG CCCTTCAGCA ACCCTTCTGA GAAGGTCTTC 
ATCCGAGGCA CTCCAGATTC TCCTGCTCAG CCAGCTGCTG CTCCCCGAGA TGCCCCTGTT 
ACCTCAGGGC CAACCAGGGC CCCACCGCCT GACTCTCCTA CCTCACTGGC CCCCACCCCC 
GCTCTTGCGC CCCCAGCCTC CCAGGCATCC ACTCTCAGCC CCTCAACTTC CTCCATGTCT 
GCCAACCAGG CCTTGTCCTC ACTCAAGGCT GTGGGTCCAC CACCCGCAAC CCCTCCACGA 
AAGCACAGGG GCCTTCTGGC CACCCAGCAA GCAGAGCCAT CCCCACCCAG TATCGTGGTC 
ACCCCAAGTG AACCCAGGTC TTTTGTCCCT GACACTGGAA CCCTGACCCC AACCTCTAGT 
CCTCAAGGGG TTAAACCAGC ACCTTCCTCA ACTTCCTTGT ATATGGTGAC TTCCTTCGTG 
TCTGCACCTC CAGCCCCCCA GGCCCCAGCC CCTGAGCCCC CTCCTGAGCC CACCAAGGTG 
ACTGTGCGGA GCCTCAGCCC AGCCAAGGAG GTGGTCAGCT CCCCAACTCC TGAGAGCACC 
ACTCTTCGGC AGGGTCCCCC TCAGAAACCC TACACCTTCC TGGAGGAGAA GGCCAGGGGC 
CGCTTTGGCG TTGTGCGGTC ATGCCGGGAG AATGCTACGG GCCGAACGTT TGTCGCCAAG 
ATCGTGCCGT ACGCCGCGGA GGGCAAGCGA CGCGTGCTGC AGGAATACGA GGTGCTTCGG 
ACGCTGCACC ACGAGCGGCT CATGTCCCTG CACGAGGCCT ACATCACCCC TCGGTACCTC 
GTGCTTATCG CTGAGAGCTG TGGCAACCGG GAACTCCTCT GTGGGCTCAG CGACAGATTC 
CGGTATTCAG AAGATGACGT GGCCACCTAT GTGGTGCAGC TGCTACAAGG CCTGGACTAC 
CTCCACGGCC ACCACGTGCT ACACCTGGAC ATCAAGCCGG ACAATCTACT GTTGGCCGCT 
GACAATGCCC TTAAAATTGT GGACTTCGGC AGTGCTCAGC CCTATAACCC TCAGGCCCTG 
AAGCCACTTG GCCACCGCAC AGGCACTCTG GAGTTCATGG CTCCTGAGAT GGTGAAGGGA 
GACCCCATTG GCTCTGCCAC GGACATCTGG GGAGCGGGTG TGCTCACCTA CATCATGCTT 
AGTGGGTACT CCCCATTCTA TGAGCCAGAC CCCCAGGAAA CAGAGGCTCG GATTGTTGGG 
GGTCGCTTTG ATGCCTTCCA GTTGTATCCT AACACATCCC AGAGTGCCAC CCTCTTCTTG 
AGAAAGGTCC TCTCAGTACA TCCCTGGAGC CGGCCCTCTC TGCAGGACTG CTTGGCCCAC 
CCATGGCTGC AAGATGCCTA CCTGATGAAG CTGCGCCGCC AGACACTCAC CTTCACCACC 
AACCGGCTCA AGGAATTCCT GGGCGAGCAG CGGCGACGTC GGGCTGAGGC TGCTACCCGT 
CACAAGGTGC TGCTCCGCTC CTACCCTGGC AGCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_011238643.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236945.1 Mus musculus SPEG complex locus (Speg), transcript variant X1, mRNA.

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CloneID	OMu67144
Clone ID Related Accession (Same CDS sequence)	XM_011238643.1 , XM_011238643.2
Accession Version	XM_011238643.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238643.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
TACGACTCGG AGACTGCCGA GGATGACATC AGCGATGTGC CGGGGACCCA GCGCCTGGAG 
CTTCGGGATG ACAGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGTACC 
TCCCTGGACA CACCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG 
GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC 
CCAGGCAGCC CAAGGCAAGC ACAGACAACC GGGGCCGGGC CACGGCACCT GGGGGTGGAG 
CCGCTGGTGC GGGCATCGCG AGCTAATCTG GTGGGCGCAA GCTGGGGGTC AGAGGATAGC 
CTTTCGGTGG CCAGTGACCT CTATGGCAGC GCGTTCAGTC TGTACAGAGG ACGGGCGCTC 
TCGATCCACG TCAGCATTCC TCCGAGTGGA TTGCACAGAG AGGAGCCTGA CCTTCAGCCT 
CAGCCAGCCA GTGATGCCCT TCGTCCACGC CCTGCCCTTC CACCTCCCTC CAAATCCGCT 
TTGCTTCCCC CACCATCTCC TCGGGTGGGA AAGAGGGCTC TGCCAGGACC TAGTACCCAG 
CCCCCAGCTA CTCCCACTTC GCCCCATCGG CGTGCTCAGG AGCCCTCGCT GCCTGAGGAC 
ATCACCACCA CGGAAGAGAA GCGAGGGAAA AAGCCCAAGT CATCCGGGCC GTCGCTGGCG 
GGCACAGTGG AGTCCCGGCC CCAGACGCCA CTGAGCGAGG CTTCGGGTCG CCTGTCAGCA 
TTGGGCCGCT CGCCCCGGCT GGTGCGCGCG GGGTCCCGCA TCCTGGACAA GCTACAGTTC 
TTCGAAGAGC GGCGACGCAG CCTGGAGCGC AGCGACTCGC CGCCAGCGCC CCTGCGGCCC 
TGGGTGCCCC TGCGCAAGGC TCGCTCGCTG GAGCAGCCGA AGTCCGAGGG CGGTGCGGCG 
TGGGGCACAC CCGAGGCCTC GCAGGAGGAG CTGCGGTCAC CTCGGGGCAG TGTGGCAGAG 
CGGCGTCGCC TGTTCCAGCA AAAGGCGGCC TCGTTGGATG AACGCACGCG ACAACGCAGT 
GCAACCTCGG ACCTCGAACT CCGCTTCGCC CAGGAGCTGG GTCGCATCCG CCGATCTACG 
TCGCGGGAGG AGCTGGTGCG TTCGCACGAG TCCCTGCGTG CCACGCTGCA GCGCGCCCCA 
TCCCCTCGGG AGCCCGGCGA GCCCCCACTC TTCTCCCGGC CTTCCACACC CAAGACCTCA 
CGGGCTGTGA GCCCGGCTGC CACCCAGCCG CCGCCTCCTA GTGGTGCGGG CAAATCTGGG 
GACGAGCCTG GGAGGCCCCG AAGCAGAGGG CCGGTGGGCA GGACTGAACC GGGGGAAGGC 
CCGCAGCAGG AGATCAAGCG TCGGGACCAA TTCCCGCTAA CCAGGAGCAG AGCCATCCAG 
GAGTGCAGGA GCCCTGTGCC GCCCTACACC GCGGATCCCC CGGAGAGCAG GACAAAAGCC 
CCCTCCGGTC GCAAGCGGGA ACCCCCTGCT CAAGCGGTGC GCTTTCTGCC CTGGGCCACT 
CCGGGAGTGG AGGACTCTGT TCTGCCCCAA ACCTTGGAGA AGAATAGAGC GGGACCCGAG 
GCTGAGAAGA GGCTTCGCAG AGGACCTGAG GAGGATGGCC CCTGGGGGCC CTGGGACCGC 
AGAGGGACCC GCAGCCAAGG CAAAGGTCGC CGTGCTCGGC CTACTTCCCC CGAGCTCGAG 
TCCTCAGACG ACTCCTATGT GTCCGCTGGG GAAGAGCCCC TGGAGGCACC CGTGTTTGAG 
ATCCCTCTGC AGAATATGGT GGTGGCGCCA GGAGCTGACG TGCTACTTAA GTGTATCATC 
ACCGCCAACC CCCCACCCCA AGTGTCCTGG AAAAAGGATG GGTCCATGTT GCACAGCGAG 
GGTCGTCTTC TCATCCGGGC TGAAGGTGAA CGGCACACAC TGCTGCTCAG AGAGGCCCAG 
GCTGCCGATG CTGGGAGCTA CACAGCCACT GCCACCAACG AACTGGGCCA AGCTACCTGT 
GCTTCTTCAC TGGCTGTGAG ACCTGGCGGC TCCACATCCC CTTTCAGCAG CCCCATCACC 
TCTGATGAGG AGTACCTGAG CCCCCCAGAG GAGTTCCCAG AGCCTGGGGA GACCTGGCCC 
CGAACCCCTA CCATGAAGCT CAGTCCCAGC CAGGATCATG ATTCCTCCGA CTCTTCTTCC 
AAGGCACCCC CAACGTTCAA GGTCTCACTC ATGGACCAAT CGGTGAGAGA AGGTCAAGAT 
GTCATTATGA GCATCCGTGT GCAGGGAGAG CCCAAGCCTG TGGTTTCCTG GCTGAGGAAT 
CGACAGCCCG TGCGCCCAGA CCAGCGGCGC TTTGCAGAGG AGGCCGAGGG TGGGCTCTGC 
CGCTTGAGGA TCCTGGCTGC TGAACGGGGC GATGCTGGTT TCTACACATG CAAGGCGGTC 
AACGAATATG GCGCTCGGCA GTGCGAGGCC CGCCTGGAGG TCCGAGCACA CCCTGAAAGC 
CGGTCCCTGG CCGTGCTGGC CCCCCTGCAG GACGTGGACG TGGGGGCCGG GGAGATGGCG 
CTGTTTGAGT GCCTGGTGGC AGGTCCAGCT GACGTCGAGG TGGACTGGCT GTGCCGGGGC 
CGCCTGCTGC AGCCTGCGCT GCTCAAATGC AAGATGCATT TTGATGGCCG CAAGTGCAAG 
CTGCTGCTCA CCTCTGTGCA CGAGGACGAC AGTGGTGTCT ACACCTGCAA GCTGAGCACA 
GCCAAAGATG AACTGACCTG CAGTGCCCGG CTGACGGTTC GGCCGTCGCT GGCGCCTCTC 
TTCACCCGGT TGCTGGAAGA TGTGGAGGTG TTGGAAGGCC GTGCTGCCCG CTTGGATTGC 
AAGATCAGCG GTACCCCGCC TCCCTCCGTT ACCTGGACTC ATTTTGGCCA CCCCGTGAAT 
GAGGGTGACA ACTTGCGGCT TCAACAGGAT GGAGGTCTAC ACTCACTGCA CATTGCCCGG 
GTGGGCAGTG AGGACGAAGG ACTCTATGAA GTCAGTGCCA CCAACACTCA TGGCCAGGCC 
CACTGCTCCG CCCAGCTGTA TGTGGAGGAG CCTCGGACAG CTGCCTCTGG CCCCAGCTCG 
AAGCTGGAGA AGATGCCATC TATCCCTGAG GAGCCGGAGC ACGGAGATCT AGAGAGGCTG 
TCTATTCCTG ACTTCCTACG GCCACTGCAA GATCTGGAAG TGGGACTAGC TAAGGAGGCC 
ATGCTGGAGT GCCAAGTGAC CGGCCTGCCC TATCCCACCA TCAGCTGGTT CCACAATGGC 
CACCGCATTC AAAGCAGTGA CGACCGACGC ATGACACAGT ACAGGGACAT ACATCGCCTG 
GTGTTCCCAG CTGTGGGGCC TCAGCATGCT GGTGTCTATA AGAGTGTAAT CGCCAACAAG 
CTGGGCAAAG CGGCCTGCTA CGCCCATCTC TATGTCACAG ATGTGGTTCC AGGCCCTCCA 
GATGGTGCTC CGGAAGTAGT AGCTGTGACC GGAAGGATGG TCACACTATC GTGGAACCCA 
CCCAGGAGTC TGGACATGGC TATTGACCCA GATTCCCTAA CATACACTGT GCAGCACCAG 
GTGTTGGGCT CGGACCAGTG GACGGCACTG GTCACAGGCC TGCGGGAGCC TGCGTGGGCA 
GCCACAGGGC TGAAGAAGGG GATCCAGCAC ATCTTCCGGG TCCTCAGCTC CAGTGGCAAG 
AGCAGCAGCA AGCCCTCAGC ACCGTCGGAA CCCGTGCAGC TGCTAGAGCA TGGCCCACCC 
CTGGAAGAGG CGCCTGCTGT GCTGGACAAG CAGGACATTG TGTATGTGGT AGAGGGCCAG 
CCTGCCTGTG TCACCGTCAC TTTTAACCAT GTGGAGGCCC AGGTTGTCTG GAGGAGCTGC 
CGGGGAGCCC TCCTCGAGGC GCGCACAGGT GTGTATGAAC TGAGTCAGCC AGATGACGAC 
CAGTATTGTC TGCGGATCTG CCGGGTGAGC CGACGAGACC TGGGACCCCT TACTTGCAGT 
GCCCGAAACC GCCACGGCAC AAAGGCCTGC TCGGTCACCC TGGAGCTGGC AGAGGCACCT 
CGGTTTGAGT CTATCATGGA AGATGTGGAG GTGGGGCCTG GAGAAACCGC TCGCTTTGCT 
GTGGTAGTTG AGGGGAAACC ACTGCCAGAC ATCATGTGGT ACAAGGATGA GGTGCTGCTG 
GCCGAGAGCA ACCATGTGAG CTTCGTGTAT GAGGAGAACG AGTGTTCCCT AGTGCTGCTC 
AGCGCAGGAA GCCAGGATGG AGGCGTCTAT ACCTGTACCG CCCGGAACCT GGCAGGCGAA 
GTCTCCTGCA AGGCGGAGCT GTCTGTGCTT TCAGCTCAGA CAGCCATGGA GGTCGAGGGG 
GTCGGAGAGG ATGAGGAACA CCGAGGAAGA AGACTTAGCG ACTACTACGA CATCCACCAA 
GAGATTGGCA GGGGTGCCTT CTCCTACCTG CGGCGTGTAG TGGAGAGGAG CTCTGGCCTG 
GAGTTTGCAG CCAAGTTCAT CCCTAGCCAG GCTAAGCCTA AAGCATCAGC AAGACGCGAG 
GCGCGCTTGC TGGCCCGGCT CCAGCATGGC TGTGTGCTCT ACTTCCACGA AGCCTTTGAG 
AGGCGCAGGG GACTTGTCAT AGTCACGGAG CTCTGCACAG AAGAGCTTCT AGAGCGCATG 
GCCAGGAAGC CCACCGTGTG TGAGTCTGAG ACTCGGACCT ATATGCGTCA AGTGCTAGAG 
GGGATATGCT ATCTGCATCA GAGCCATGTG CTGCACCTGG ATGTCAAGCC GGAGAACCTG 
CTTGTGTGGG ATGGTGCAGG GGGTGAAGAG CAGGTGCGCA TCTGTGACTT CGGGAACGCC 
CAGGAACTCA CTCCGGGAGA GCCCCAGTAC TGTCAGTATG GCACGCCAGA GTTTGTAGCA 
CCTGAGATTG TCAACCAGAG CCCTGTGTCT GGAGTCACTG ACATCTGGCC CGTGGGCGTC 
GTTGCCTTTC TCTGTTTGAC GGGCATCTCC CCATTTGTTG GGGAGAATGA CCGGACAACA 
CTCATGAATA TCCGAAACTA CAACGTGGCA TTTGAGGAGA CTACGTTCCT GAGTCTGAGC 
AGGGAGGCCC GGGGCTTCCT CATCAAAGTG CTGGTGCAGG ATCGACTGAG ACCTACTGCA 
GAAGAGACAT TGGAACATCC TTGGTTCAAA ACAGAAGCAA AGGGTGCAGA GGTGAGCACA 
GATCACCTGA AGCTCTTCCT GTCACGGAGG AGGTGGCAGC GCTCTCAAAT CAGCTACAAG 
TGCCACCTGG TGCTGCGCCC CATTCCGGAA CTGCTACGGG CTCCCCCGGA GCGGGTGTGG 
GTGGCCATGC CTAGAAGACA ACCCCCGAGT GGGGGACTCT CATCCTCTTC AGACTCAGAA 
GAGGAAGAGT TGGAGGAACT TCCCTCAGTA CCCCGCCCCC TGCAGCCTGA GTTCTCAGGT 
TCTCGGGTAT CCCTCACCGA CATCCCCACC GAAGATGAGG CTCTGGGGAC CCCGGAAGCT 
GGGGCTGCCA CCCCCATGGA CTGGCAAGAG CAGGAAAGGA CTCCCTCTAA GGACCAGGAG 
GCCCCCAGCC CTGAGGCACT CCCCTCTCCA GGCCAGGAGT CCCCTGATGG GCCTAGCCCC 
AGGCGGCCAG AGCTCCGCAG GGGTAGCTCT GCTGAGAGTG CCCTGCCCCG AGTCGGGTCG 
CGGGAACCGG GCCGGAGCTT GCACAAGGCA GCATCCGTGG AGCTGCCGCA GCGCAGGAGC 
CCCAGCCCGG GTGCCACCCG CCTGACTCGG GGAGGCCTGG GTGAGGGCGA GTATGCCCAG 
AGGCTGCAGG CCCTGCGTCA GCGGCTGCTG AGGGGAGGCC CCGAGGATGG CAAGGTCAGC 
GGCCTCAGGG GACCCCTGCT GGAGAGCCTG GGGGGCCGTG CACGGGATCC CAGGATGGCA 
AGGGCTGCCT CCAGCGAGGC TGCCCCCCAC CACCAGCCCC CTCCGGAGTC CCGGGGTCTG 
CAAAAGAGCA GCAGCTTCTC TCAGGGCGAG GCAGAGCCCC GGGGTCGGCA CCGCCGCGCG 
GGGGCACCCC TCGAAATCCC CGTGGCCAGA CTTGGGGCTC GCAGGCTGCA GGAGTCTCCC 
TCCTTATCTG CCCTTAGCGA GACCCAGCCA CCGAGCCCTG CGAGGCCCAG CGTCCCCAAA 
CTCAGTATCA CCAAGTCCCC GGAACCTTCC GCTGTCACAT CCAGAGACTC TCCCCAGCCC 
CCGGAACCTC AGCCTGTCCC AGAAAAGGTC CCTGAGCCCA AACCAGAACC AGTCCGAGCT 
GCAAAACCAG CACAACCCCC TTTGGCTCTG CAAATGCCAA CGCAGCCTCT CACCCCCTAT 
GCCCAGATCA TGCAGTCGCT CCAGCTGTCC AGTCCCACCC TGTCCCCACA GGACCCCGCT 
GTGCCTCCAT CAGAGCCCAA GCCCCACGCG GCTGTGTTTG CCAGGGTGGC TTCCCCACCA 
CCCGGAGTCT CTGAGAAGCG CGTGCCTTCG GCCAGAACTC CCCCAGTGCT AGCCGAGAAA 
GCCCGAGTCC CCACGGTGCC CCCCAGGCCA GGCAGCAGCC TCAGCGGCAG CATCGAAAAC 
CTTGAATCAG AGGCTGTGTT CGAGGCTAAG TTCAAACGCA GCCGCGAATC GCCACTGTCG 
CGGGGCCTGA GGCTACTGAG CCGCTCGCGC TCGGAGGAGC GCGGTCCCTT CCGTGGGGCG 
GAGGACGACG GCATATACCG GCCCAGTCCG GCAGGAACGC CGCTAGAGCT GGTGCGACGG 
CCCGAGCGCT CGCGCTCCGT GCAGGACCTC AGGGTAGCCG GAGAGCCGGG CCTTGTGCGT 
CGCCTTTCGC TGTCCCTGTC GCAGAAGCTG CGGCGGACAC CGCCGGGACA GCGACACCCT 
GCCTGGGAGT CCCGCAGCGG AGACGGGGAG AGCTCCGAGG GCGGCAGCTC AGCTCGGGCA 
TCCCCAGTGC TGGCGGTACG CAGGAGACTC AGCTCCACGC TGGAGCGTCT GTCCAGCCGT 
TTGCAGCGCA GCGGCAGCAG CGAGGACTCC GGGGGCGCCT CGGGTCGCAG CACGCCGCTG 
TTTGGACGGC TGCGCAGGGC TACTTCGGAA GGGGAGAGCC TGCGACGCCT CGGTGTTCCG 
CACAATCAGC TGGGCTCTCA GACTGGTGCC ACTACACCTT CCGCTGAGTC TCTGGGCTCA 
GAGGCCAGTG GCACATCGGG TTCTTCAGCT CCTGGAGAAA GCCGAAGCCG GCACCGGTGG 
GGCCTTTCCA GGCTGCGCAA GGACAAGGGA TTATCTCAGC CAAACCTCTC CTCCAGTGTC 
CAAGAGGACT TGGGTCACCA GTATGTGCCC AGTGAGTCAG ATTTCCCCCC AGTCTTCCAC 
ATCAAACTCA AGGACCAGGT GCTGTTGGAG GGGGAAGCAG CCACCCTGCT CTGCCTACCC 
GCAGCCTGCC CAGCACCCCG CATCTCCTGG ATGAAAGATA AGCAGTCCCT GCGTTCAGAG 
CCCTCAGTGG TCATTGTGTC CTGCAAAGAT GGACGGCAGC TGCTGAGCAT ACCCCGGGCC 
GGCAAGCGGC ATGCTGGGCT CTATGAGTGC TCAGCCACCA ATGTCCTGGG CAGCATTACC 
AGCTCCTGTA CTGTGGCCGT GGCCCGCATT CCAGGGAAGC TAGCTCCTCC AGAGGTGCCC 
CAGACCTACC ATGACACAGC ACTAGTAGTG TGGAAGCCAG GAGACGGTCG GGCACCTTGC 
ACATACACTC TGGAGCGGCG GGTGGATGGG GAATCTGTCT GGCACCCTGT GAGCTCAGGC 
ATCCCCGACT GTTATTACAA TGTGACTCAG CTGCCTGTCG GCGTGACCGT GCGGTTCCGT 
GTGGCCTGTT CCAATCGTGC TGGGCAGGGG CCCTTCAGCA ACCCTTCTGA GAAGGTCTTC 
ATCCGAGGCA CTCCAGATTC TCCTGCTCAG CCAGCTGCTG CTCCCCGAGA TGCCCCTGTT 
ACCTCAGGGC CAACCAGGGC CCCACCGCCT GACTCTCCTA CCTCACTGGC CCCCACCCCC 
GCTCTTGCGC CCCCAGCCTC CCAGGCATCC ACTCTCAGCC CCTCAACTTC CTCCATGTCT 
GCCAACCAGG CCTTGTCCTC ACTCAAGGCT GTGGGTCCAC CACCCGCAAC CCCTCCACGA 
AAGCACAGGG GCCTTCTGGC CACCCAGCAA GCAGAGCCAT CCCCACCCAG TATCGTGGTC 
ACCCCAAGTG AACCCAGGTC TTTTGTCCCT GACACTGGAA CCCTGACCCC AACCTCTAGT 
CCTCAAGGGG TTAAACCAGC ACCTTCCTCA ACTTCCTTGT ATATGGTGAC TTCCTTCGTG 
TCTGCACCTC CAGCCCCCCA GGCCCCAGCC CCTGAGCCCC CTCCTGAGCC CACCAAGGTG 
ACTGTGCGGA GCCTCAGCCC AGCCAAGGAG GTGGTCAGCT CCCCAACTCC TGAGAGCACC 
ACTCTTCGGC AGGGTCCCCC TCAGAAACCC TACACCTTCC TGGAGGAGAA GGCCAGGGGC 
CGCTTTGGCG TTGTGCGGTC ATGCCGGGAG AATGCTACGG GCCGAACGTT TGTCGCCAAG 
ATCGTGCCGT ACGCCGCGGA GGGCAAGCGA CGCGTGCTGC AGGAATACGA GGTGCTTCGG 
ACGCTGCACC ACGAGCGGCT CATGTCCCTG CACGAGGCCT ACATCACCCC TCGGTACCTC 
GTGCTTATCG CTGAGAGCTG TGGCAACCGG GAACTCCTCT GTGGGCTCAG CGACAGATTC 
CGGTATTCAG AAGATGACGT GGCCACCTAT GTGGTGCAGC TGCTACAAGG CCTGGACTAC 
CTCCACGGCC ACCACGTGCT ACACCTGGAC ATCAAGCCGG ACAATCTACT GTTGGCCGCT 
GACAATGCCC TTAAAATTGT GGACTTCGGC AGTGCTCAGC CCTATAACCC TCAGGCCCTG 
AAGCCACTTG GCCACCGCAC AGGCACTCTG GAGTTCATGG CTCCTGAGAT GGTGAAGGGA 
GACCCCATTG GCTCTGCCAC GGACATCTGG GGAGCGGGTG TGCTCACCTA CATCATGCTT 
AGTGGGTACT CCCCATTCTA TGAGCCAGAC CCCCAGGAAA CAGAGGCTCG GATTGTTGGG 
GGTCGCTTTG ATGCCTTCCA GTTGTATCCT AACACATCCC AGAGTGCCAC CCTCTTCTTG 
AGAAAGGTCC TCTCAGTACA TCCCTGGAGC CGGCCCTCTC TGCAGGACTG CTTGGCCCAC 
CCATGGCTGC AAGATGCCTA CCTGATGAAG CTGCGCCGCC AGACACTCAC CTTCACCACC 
AACCGGCTCA AGGAATTCCT GGGCGAGCAG CGGCGACGTC GGGCTGAGGC TGCTACCCGT 
CACAAGGTGC TGCTCCGCTC CTACCCTGGC AGCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236945.1
CDS	103..9921
Translation	MQKARGTRGEDAGTRAPPSPGVPPKRAKVGAGRGVLVTGDGAGAPVFLRPLKNAAVCAGSDVRLRVVVSGTPQPSLSWFRDGQLLPPPAPEPSCLWLRSCGAQDAGVYSCSAQNERGQASCEAVLTVLEVRVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPRQAQTTGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_011238643.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238643.2

ORF Insert Sequence:

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
TACGACTCGG AGACTGCCGA GGATGACATC AGCGATGTGC CGGGGACCCA GCGCCTGGAG 
CTTCGGGATG ACAGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGTACC 
TCCCTGGACA CACCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG 
GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC 
CCAGGCAGCC CAAGGCAAGC ACAGACAACC GGGGCCGGGC CACGGCACCT GGGGGTGGAG 
CCGCTGGTGC GGGCATCGCG AGCTAATCTG GTGGGCGCAA GCTGGGGGTC AGAGGATAGC 
CTTTCGGTGG CCAGTGACCT CTATGGCAGC GCGTTCAGTC TGTACAGAGG ACGGGCGCTC 
TCGATCCACG TCAGCATTCC TCCGAGTGGA TTGCACAGAG AGGAGCCTGA CCTTCAGCCT 
CAGCCAGCCA GTGATGCCCT TCGTCCACGC CCTGCCCTTC CACCTCCCTC CAAATCCGCT 
TTGCTTCCCC CACCATCTCC TCGGGTGGGA AAGAGGGCTC TGCCAGGACC TAGTACCCAG 
CCCCCAGCTA CTCCCACTTC GCCCCATCGG CGTGCTCAGG AGCCCTCGCT GCCTGAGGAC 
ATCACCACCA CGGAAGAGAA GCGAGGGAAA AAGCCCAAGT CATCCGGGCC GTCGCTGGCG 
GGCACAGTGG AGTCCCGGCC CCAGACGCCA CTGAGCGAGG CTTCGGGTCG CCTGTCAGCA 
TTGGGCCGCT CGCCCCGGCT GGTGCGCGCG GGGTCCCGCA TCCTGGACAA GCTACAGTTC 
TTCGAAGAGC GGCGACGCAG CCTGGAGCGC AGCGACTCGC CGCCAGCGCC CCTGCGGCCC 
TGGGTGCCCC TGCGCAAGGC TCGCTCGCTG GAGCAGCCGA AGTCCGAGGG CGGTGCGGCG 
TGGGGCACAC CCGAGGCCTC GCAGGAGGAG CTGCGGTCAC CTCGGGGCAG TGTGGCAGAG 
CGGCGTCGCC TGTTCCAGCA AAAGGCGGCC TCGTTGGATG AACGCACGCG ACAACGCAGT 
GCAACCTCGG ACCTCGAACT CCGCTTCGCC CAGGAGCTGG GTCGCATCCG CCGATCTACG 
TCGCGGGAGG AGCTGGTGCG TTCGCACGAG TCCCTGCGTG CCACGCTGCA GCGCGCCCCA 
TCCCCTCGGG AGCCCGGCGA GCCCCCACTC TTCTCCCGGC CTTCCACACC CAAGACCTCA 
CGGGCTGTGA GCCCGGCTGC CACCCAGCCG CCGCCTCCTA GTGGTGCGGG CAAATCTGGG 
GACGAGCCTG GGAGGCCCCG AAGCAGAGGG CCGGTGGGCA GGACTGAACC GGGGGAAGGC 
CCGCAGCAGG AGATCAAGCG TCGGGACCAA TTCCCGCTAA CCAGGAGCAG AGCCATCCAG 
GAGTGCAGGA GCCCTGTGCC GCCCTACACC GCGGATCCCC CGGAGAGCAG GACAAAAGCC 
CCCTCCGGTC GCAAGCGGGA ACCCCCTGCT CAAGCGGTGC GCTTTCTGCC CTGGGCCACT 
CCGGGAGTGG AGGACTCTGT TCTGCCCCAA ACCTTGGAGA AGAATAGAGC GGGACCCGAG 
GCTGAGAAGA GGCTTCGCAG AGGACCTGAG GAGGATGGCC CCTGGGGGCC CTGGGACCGC 
AGAGGGACCC GCAGCCAAGG CAAAGGTCGC CGTGCTCGGC CTACTTCCCC CGAGCTCGAG 
TCCTCAGACG ACTCCTATGT GTCCGCTGGG GAAGAGCCCC TGGAGGCACC CGTGTTTGAG 
ATCCCTCTGC AGAATATGGT GGTGGCGCCA GGAGCTGACG TGCTACTTAA GTGTATCATC 
ACCGCCAACC CCCCACCCCA AGTGTCCTGG AAAAAGGATG GGTCCATGTT GCACAGCGAG 
GGTCGTCTTC TCATCCGGGC TGAAGGTGAA CGGCACACAC TGCTGCTCAG AGAGGCCCAG 
GCTGCCGATG CTGGGAGCTA CACAGCCACT GCCACCAACG AACTGGGCCA AGCTACCTGT 
GCTTCTTCAC TGGCTGTGAG ACCTGGCGGC TCCACATCCC CTTTCAGCAG CCCCATCACC 
TCTGATGAGG AGTACCTGAG CCCCCCAGAG GAGTTCCCAG AGCCTGGGGA GACCTGGCCC 
CGAACCCCTA CCATGAAGCT CAGTCCCAGC CAGGATCATG ATTCCTCCGA CTCTTCTTCC 
AAGGCACCCC CAACGTTCAA GGTCTCACTC ATGGACCAAT CGGTGAGAGA AGGTCAAGAT 
GTCATTATGA GCATCCGTGT GCAGGGAGAG CCCAAGCCTG TGGTTTCCTG GCTGAGGAAT 
CGACAGCCCG TGCGCCCAGA CCAGCGGCGC TTTGCAGAGG AGGCCGAGGG TGGGCTCTGC 
CGCTTGAGGA TCCTGGCTGC TGAACGGGGC GATGCTGGTT TCTACACATG CAAGGCGGTC 
AACGAATATG GCGCTCGGCA GTGCGAGGCC CGCCTGGAGG TCCGAGCACA CCCTGAAAGC 
CGGTCCCTGG CCGTGCTGGC CCCCCTGCAG GACGTGGACG TGGGGGCCGG GGAGATGGCG 
CTGTTTGAGT GCCTGGTGGC AGGTCCAGCT GACGTCGAGG TGGACTGGCT GTGCCGGGGC 
CGCCTGCTGC AGCCTGCGCT GCTCAAATGC AAGATGCATT TTGATGGCCG CAAGTGCAAG 
CTGCTGCTCA CCTCTGTGCA CGAGGACGAC AGTGGTGTCT ACACCTGCAA GCTGAGCACA 
GCCAAAGATG AACTGACCTG CAGTGCCCGG CTGACGGTTC GGCCGTCGCT GGCGCCTCTC 
TTCACCCGGT TGCTGGAAGA TGTGGAGGTG TTGGAAGGCC GTGCTGCCCG CTTGGATTGC 
AAGATCAGCG GTACCCCGCC TCCCTCCGTT ACCTGGACTC ATTTTGGCCA CCCCGTGAAT 
GAGGGTGACA ACTTGCGGCT TCAACAGGAT GGAGGTCTAC ACTCACTGCA CATTGCCCGG 
GTGGGCAGTG AGGACGAAGG ACTCTATGAA GTCAGTGCCA CCAACACTCA TGGCCAGGCC 
CACTGCTCCG CCCAGCTGTA TGTGGAGGAG CCTCGGACAG CTGCCTCTGG CCCCAGCTCG 
AAGCTGGAGA AGATGCCATC TATCCCTGAG GAGCCGGAGC ACGGAGATCT AGAGAGGCTG 
TCTATTCCTG ACTTCCTACG GCCACTGCAA GATCTGGAAG TGGGACTAGC TAAGGAGGCC 
ATGCTGGAGT GCCAAGTGAC CGGCCTGCCC TATCCCACCA TCAGCTGGTT CCACAATGGC 
CACCGCATTC AAAGCAGTGA CGACCGACGC ATGACACAGT ACAGGGACAT ACATCGCCTG 
GTGTTCCCAG CTGTGGGGCC TCAGCATGCT GGTGTCTATA AGAGTGTAAT CGCCAACAAG 
CTGGGCAAAG CGGCCTGCTA CGCCCATCTC TATGTCACAG ATGTGGTTCC AGGCCCTCCA 
GATGGTGCTC CGGAAGTAGT AGCTGTGACC GGAAGGATGG TCACACTATC GTGGAACCCA 
CCCAGGAGTC TGGACATGGC TATTGACCCA GATTCCCTAA CATACACTGT GCAGCACCAG 
GTGTTGGGCT CGGACCAGTG GACGGCACTG GTCACAGGCC TGCGGGAGCC TGCGTGGGCA 
GCCACAGGGC TGAAGAAGGG GATCCAGCAC ATCTTCCGGG TCCTCAGCTC CAGTGGCAAG 
AGCAGCAGCA AGCCCTCAGC ACCGTCGGAA CCCGTGCAGC TGCTAGAGCA TGGCCCACCC 
CTGGAAGAGG CGCCTGCTGT GCTGGACAAG CAGGACATTG TGTATGTGGT AGAGGGCCAG 
CCTGCCTGTG TCACCGTCAC TTTTAACCAT GTGGAGGCCC AGGTTGTCTG GAGGAGCTGC 
CGGGGAGCCC TCCTCGAGGC GCGCACAGGT GTGTATGAAC TGAGTCAGCC AGATGACGAC 
CAGTATTGTC TGCGGATCTG CCGGGTGAGC CGACGAGACC TGGGACCCCT TACTTGCAGT 
GCCCGAAACC GCCACGGCAC AAAGGCCTGC TCGGTCACCC TGGAGCTGGC AGAGGCACCT 
CGGTTTGAGT CTATCATGGA AGATGTGGAG GTGGGGCCTG GAGAAACCGC TCGCTTTGCT 
GTGGTAGTTG AGGGGAAACC ACTGCCAGAC ATCATGTGGT ACAAGGATGA GGTGCTGCTG 
GCCGAGAGCA ACCATGTGAG CTTCGTGTAT GAGGAGAACG AGTGTTCCCT AGTGCTGCTC 
AGCGCAGGAA GCCAGGATGG AGGCGTCTAT ACCTGTACCG CCCGGAACCT GGCAGGCGAA 
GTCTCCTGCA AGGCGGAGCT GTCTGTGCTT TCAGCTCAGA CAGCCATGGA GGTCGAGGGG 
GTCGGAGAGG ATGAGGAACA CCGAGGAAGA AGACTTAGCG ACTACTACGA CATCCACCAA 
GAGATTGGCA GGGGTGCCTT CTCCTACCTG CGGCGTGTAG TGGAGAGGAG CTCTGGCCTG 
GAGTTTGCAG CCAAGTTCAT CCCTAGCCAG GCTAAGCCTA AAGCATCAGC AAGACGCGAG 
GCGCGCTTGC TGGCCCGGCT CCAGCATGGC TGTGTGCTCT ACTTCCACGA AGCCTTTGAG 
AGGCGCAGGG GACTTGTCAT AGTCACGGAG CTCTGCACAG AAGAGCTTCT AGAGCGCATG 
GCCAGGAAGC CCACCGTGTG TGAGTCTGAG ACTCGGACCT ATATGCGTCA AGTGCTAGAG 
GGGATATGCT ATCTGCATCA GAGCCATGTG CTGCACCTGG ATGTCAAGCC GGAGAACCTG 
CTTGTGTGGG ATGGTGCAGG GGGTGAAGAG CAGGTGCGCA TCTGTGACTT CGGGAACGCC 
CAGGAACTCA CTCCGGGAGA GCCCCAGTAC TGTCAGTATG GCACGCCAGA GTTTGTAGCA 
CCTGAGATTG TCAACCAGAG CCCTGTGTCT GGAGTCACTG ACATCTGGCC CGTGGGCGTC 
GTTGCCTTTC TCTGTTTGAC GGGCATCTCC CCATTTGTTG GGGAGAATGA CCGGACAACA 
CTCATGAATA TCCGAAACTA CAACGTGGCA TTTGAGGAGA CTACGTTCCT GAGTCTGAGC 
AGGGAGGCCC GGGGCTTCCT CATCAAAGTG CTGGTGCAGG ATCGACTGAG ACCTACTGCA 
GAAGAGACAT TGGAACATCC TTGGTTCAAA ACAGAAGCAA AGGGTGCAGA GGTGAGCACA 
GATCACCTGA AGCTCTTCCT GTCACGGAGG AGGTGGCAGC GCTCTCAAAT CAGCTACAAG 
TGCCACCTGG TGCTGCGCCC CATTCCGGAA CTGCTACGGG CTCCCCCGGA GCGGGTGTGG 
GTGGCCATGC CTAGAAGACA ACCCCCGAGT GGGGGACTCT CATCCTCTTC AGACTCAGAA 
GAGGAAGAGT TGGAGGAACT TCCCTCAGTA CCCCGCCCCC TGCAGCCTGA GTTCTCAGGT 
TCTCGGGTAT CCCTCACCGA CATCCCCACC GAAGATGAGG CTCTGGGGAC CCCGGAAGCT 
GGGGCTGCCA CCCCCATGGA CTGGCAAGAG CAGGAAAGGA CTCCCTCTAA GGACCAGGAG 
GCCCCCAGCC CTGAGGCACT CCCCTCTCCA GGCCAGGAGT CCCCTGATGG GCCTAGCCCC 
AGGCGGCCAG AGCTCCGCAG GGGTAGCTCT GCTGAGAGTG CCCTGCCCCG AGTCGGGTCG 
CGGGAACCGG GCCGGAGCTT GCACAAGGCA GCATCCGTGG AGCTGCCGCA GCGCAGGAGC 
CCCAGCCCGG GTGCCACCCG CCTGACTCGG GGAGGCCTGG GTGAGGGCGA GTATGCCCAG 
AGGCTGCAGG CCCTGCGTCA GCGGCTGCTG AGGGGAGGCC CCGAGGATGG CAAGGTCAGC 
GGCCTCAGGG GACCCCTGCT GGAGAGCCTG GGGGGCCGTG CACGGGATCC CAGGATGGCA 
AGGGCTGCCT CCAGCGAGGC TGCCCCCCAC CACCAGCCCC CTCCGGAGTC CCGGGGTCTG 
CAAAAGAGCA GCAGCTTCTC TCAGGGCGAG GCAGAGCCCC GGGGTCGGCA CCGCCGCGCG 
GGGGCACCCC TCGAAATCCC CGTGGCCAGA CTTGGGGCTC GCAGGCTGCA GGAGTCTCCC 
TCCTTATCTG CCCTTAGCGA GACCCAGCCA CCGAGCCCTG CGAGGCCCAG CGTCCCCAAA 
CTCAGTATCA CCAAGTCCCC GGAACCTTCC GCTGTCACAT CCAGAGACTC TCCCCAGCCC 
CCGGAACCTC AGCCTGTCCC AGAAAAGGTC CCTGAGCCCA AACCAGAACC AGTCCGAGCT 
GCAAAACCAG CACAACCCCC TTTGGCTCTG CAAATGCCAA CGCAGCCTCT CACCCCCTAT 
GCCCAGATCA TGCAGTCGCT CCAGCTGTCC AGTCCCACCC TGTCCCCACA GGACCCCGCT 
GTGCCTCCAT CAGAGCCCAA GCCCCACGCG GCTGTGTTTG CCAGGGTGGC TTCCCCACCA 
CCCGGAGTCT CTGAGAAGCG CGTGCCTTCG GCCAGAACTC CCCCAGTGCT AGCCGAGAAA 
GCCCGAGTCC CCACGGTGCC CCCCAGGCCA GGCAGCAGCC TCAGCGGCAG CATCGAAAAC 
CTTGAATCAG AGGCTGTGTT CGAGGCTAAG TTCAAACGCA GCCGCGAATC GCCACTGTCG 
CGGGGCCTGA GGCTACTGAG CCGCTCGCGC TCGGAGGAGC GCGGTCCCTT CCGTGGGGCG 
GAGGACGACG GCATATACCG GCCCAGTCCG GCAGGAACGC CGCTAGAGCT GGTGCGACGG 
CCCGAGCGCT CGCGCTCCGT GCAGGACCTC AGGGTAGCCG GAGAGCCGGG CCTTGTGCGT 
CGCCTTTCGC TGTCCCTGTC GCAGAAGCTG CGGCGGACAC CGCCGGGACA GCGACACCCT 
GCCTGGGAGT CCCGCAGCGG AGACGGGGAG AGCTCCGAGG GCGGCAGCTC AGCTCGGGCA 
TCCCCAGTGC TGGCGGTACG CAGGAGACTC AGCTCCACGC TGGAGCGTCT GTCCAGCCGT 
TTGCAGCGCA GCGGCAGCAG CGAGGACTCC GGGGGCGCCT CGGGTCGCAG CACGCCGCTG 
TTTGGACGGC TGCGCAGGGC TACTTCGGAA GGGGAGAGCC TGCGACGCCT CGGTGTTCCG 
CACAATCAGC TGGGCTCTCA GACTGGTGCC ACTACACCTT CCGCTGAGTC TCTGGGCTCA 
GAGGCCAGTG GCACATCGGG TTCTTCAGCT CCTGGAGAAA GCCGAAGCCG GCACCGGTGG 
GGCCTTTCCA GGCTGCGCAA GGACAAGGGA TTATCTCAGC CAAACCTCTC CTCCAGTGTC 
CAAGAGGACT TGGGTCACCA GTATGTGCCC AGTGAGTCAG ATTTCCCCCC AGTCTTCCAC 
ATCAAACTCA AGGACCAGGT GCTGTTGGAG GGGGAAGCAG CCACCCTGCT CTGCCTACCC 
GCAGCCTGCC CAGCACCCCG CATCTCCTGG ATGAAAGATA AGCAGTCCCT GCGTTCAGAG 
CCCTCAGTGG TCATTGTGTC CTGCAAAGAT GGACGGCAGC TGCTGAGCAT ACCCCGGGCC 
GGCAAGCGGC ATGCTGGGCT CTATGAGTGC TCAGCCACCA ATGTCCTGGG CAGCATTACC 
AGCTCCTGTA CTGTGGCCGT GGCCCGCATT CCAGGGAAGC TAGCTCCTCC AGAGGTGCCC 
CAGACCTACC ATGACACAGC ACTAGTAGTG TGGAAGCCAG GAGACGGTCG GGCACCTTGC 
ACATACACTC TGGAGCGGCG GGTGGATGGG GAATCTGTCT GGCACCCTGT GAGCTCAGGC 
ATCCCCGACT GTTATTACAA TGTGACTCAG CTGCCTGTCG GCGTGACCGT GCGGTTCCGT 
GTGGCCTGTT CCAATCGTGC TGGGCAGGGG CCCTTCAGCA ACCCTTCTGA GAAGGTCTTC 
ATCCGAGGCA CTCCAGATTC TCCTGCTCAG CCAGCTGCTG CTCCCCGAGA TGCCCCTGTT 
ACCTCAGGGC CAACCAGGGC CCCACCGCCT GACTCTCCTA CCTCACTGGC CCCCACCCCC 
GCTCTTGCGC CCCCAGCCTC CCAGGCATCC ACTCTCAGCC CCTCAACTTC CTCCATGTCT 
GCCAACCAGG CCTTGTCCTC ACTCAAGGCT GTGGGTCCAC CACCCGCAAC CCCTCCACGA 
AAGCACAGGG GCCTTCTGGC CACCCAGCAA GCAGAGCCAT CCCCACCCAG TATCGTGGTC 
ACCCCAAGTG AACCCAGGTC TTTTGTCCCT GACACTGGAA CCCTGACCCC AACCTCTAGT 
CCTCAAGGGG TTAAACCAGC ACCTTCCTCA ACTTCCTTGT ATATGGTGAC TTCCTTCGTG 
TCTGCACCTC CAGCCCCCCA GGCCCCAGCC CCTGAGCCCC CTCCTGAGCC CACCAAGGTG 
ACTGTGCGGA GCCTCAGCCC AGCCAAGGAG GTGGTCAGCT CCCCAACTCC TGAGAGCACC 
ACTCTTCGGC AGGGTCCCCC TCAGAAACCC TACACCTTCC TGGAGGAGAA GGCCAGGGGC 
CGCTTTGGCG TTGTGCGGTC ATGCCGGGAG AATGCTACGG GCCGAACGTT TGTCGCCAAG 
ATCGTGCCGT ACGCCGCGGA GGGCAAGCGA CGCGTGCTGC AGGAATACGA GGTGCTTCGG 
ACGCTGCACC ACGAGCGGCT CATGTCCCTG CACGAGGCCT ACATCACCCC TCGGTACCTC 
GTGCTTATCG CTGAGAGCTG TGGCAACCGG GAACTCCTCT GTGGGCTCAG CGACAGATTC 
CGGTATTCAG AAGATGACGT GGCCACCTAT GTGGTGCAGC TGCTACAAGG CCTGGACTAC 
CTCCACGGCC ACCACGTGCT ACACCTGGAC ATCAAGCCGG ACAATCTACT GTTGGCCGCT 
GACAATGCCC TTAAAATTGT GGACTTCGGC AGTGCTCAGC CCTATAACCC TCAGGCCCTG 
AAGCCACTTG GCCACCGCAC AGGCACTCTG GAGTTCATGG CTCCTGAGAT GGTGAAGGGA 
GACCCCATTG GCTCTGCCAC GGACATCTGG GGAGCGGGTG TGCTCACCTA CATCATGCTT 
AGTGGGTACT CCCCATTCTA TGAGCCAGAC CCCCAGGAAA CAGAGGCTCG GATTGTTGGG 
GGTCGCTTTG ATGCCTTCCA GTTGTATCCT AACACATCCC AGAGTGCCAC CCTCTTCTTG 
AGAAAGGTCC TCTCAGTACA TCCCTGGAGC CGGCCCTCTC TGCAGGACTG CTTGGCCCAC 
CCATGGCTGC AAGATGCCTA CCTGATGAAG CTGCGCCGCC AGACACTCAC CTTCACCACC 
AACCGGCTCA AGGAATTCCT GGGCGAGCAG CGGCGACGTC GGGCTGAGGC TGCTACCCGT 
CACAAGGTGC TGCTCCGCTC CTACCCTGGC AGCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_011238644.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236946.1 Mus musculus SPEG complex locus (Speg), transcript variant X2, mRNA.

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CloneID	OMu67145
Clone ID Related Accession (Same CDS sequence)	XM_011238644.1 , XM_011238644.2
Accession Version	XM_011238644.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
TACGACTCGG AGACTGCCGA GGATGACATC AGCGATGTGC CGGGGACCCA GCGCCTGGAG 
CTTCGGGATG ACAGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGTACC 
TCCCTGGACA CACCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG 
GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC 
CCAGGCAGCC CAAGCAGCAT TCCTCCGAGT GGATTGCACA GAGAGGAGCC TGACCTTCAG 
CCTCAGCCAG CCAGTGATGC CCTTCGTCCA CGCCCTGCCC TTCCACCTCC CTCCAAATCC 
GCTTTGCTTC CCCCACCATC TCCTCGGGTG GGAAAGAGGG CTCTGCCAGG ACCTAGTACC 
CAGCCCCCAG CTACTCCCAC TTCGCCCCAT CGGCGTGCTC AGGAGCCCTC GCTGCCTGAG 
GACATCACCA CCACGGAAGA GAAGCGAGGG AAAAAGCCCA AGTCATCCGG GCCGTCGCTG 
GCGGGCACAG TGGAGTCCCG GCCCCAGACG CCACTGAGCG AGGCTTCGGG TCGCCTGTCA 
GCATTGGGCC GCTCGCCCCG GCTGGTGCGC GCGGGGTCCC GCATCCTGGA CAAGCTACAG 
TTCTTCGAAG AGCGGCGACG CAGCCTGGAG CGCAGCGACT CGCCGCCAGC GCCCCTGCGG 
CCCTGGGTGC CCCTGCGCAA GGCTCGCTCG CTGGAGCAGC CGAAGTCCGA GGGCGGTGCG 
GCGTGGGGCA CACCCGAGGC CTCGCAGGAG GAGCTGCGGT CACCTCGGGG CAGTGTGGCA 
GAGCGGCGTC GCCTGTTCCA GCAAAAGGCG GCCTCGTTGG ATGAACGCAC GCGACAACGC 
AGTGCAACCT CGGACCTCGA ACTCCGCTTC GCCCAGGAGC TGGGTCGCAT CCGCCGATCT 
ACGTCGCGGG AGGAGCTGGT GCGTTCGCAC GAGTCCCTGC GTGCCACGCT GCAGCGCGCC 
CCATCCCCTC GGGAGCCCGG CGAGCCCCCA CTCTTCTCCC GGCCTTCCAC ACCCAAGACC 
TCACGGGCTG TGAGCCCGGC TGCCACCCAG CCGCCGCCTC CTAGTGGTGC GGGCAAATCT 
GGGGACGAGC CTGGGAGGCC CCGAAGCAGA GGGCCGGTGG GCAGGACTGA ACCGGGGGAA 
GGCCCGCAGC AGGAGATCAA GCGTCGGGAC CAATTCCCGC TAACCAGGAG CAGAGCCATC 
CAGGAGTGCA GGAGCCCTGT GCCGCCCTAC ACCGCGGATC CCCCGGAGAG CAGGACAAAA 
GCCCCCTCCG GTCGCAAGCG GGAACCCCCT GCTCAAGCGG TGCGCTTTCT GCCCTGGGCC 
ACTCCGGGAG TGGAGGACTC TGTTCTGCCC CAAACCTTGG AGAAGAATAG AGCGGGACCC 
GAGGCTGAGA AGAGGCTTCG CAGAGGACCT GAGGAGGATG GCCCCTGGGG GCCCTGGGAC 
CGCAGAGGGA CCCGCAGCCA AGGCAAAGGT CGCCGTGCTC GGCCTACTTC CCCCGAGCTC 
GAGTCCTCAG ACGACTCCTA TGTGTCCGCT GGGGAAGAGC CCCTGGAGGC ACCCGTGTTT 
GAGATCCCTC TGCAGAATAT GGTGGTGGCG CCAGGAGCTG ACGTGCTACT TAAGTGTATC 
ATCACCGCCA ACCCCCCACC CCAAGTGTCC TGGAAAAAGG ATGGGTCCAT GTTGCACAGC 
GAGGGTCGTC TTCTCATCCG GGCTGAAGGT GAACGGCACA CACTGCTGCT CAGAGAGGCC 
CAGGCTGCCG ATGCTGGGAG CTACACAGCC ACTGCCACCA ACGAACTGGG CCAAGCTACC 
TGTGCTTCTT CACTGGCTGT GAGACCTGGC GGCTCCACAT CCCCTTTCAG CAGCCCCATC 
ACCTCTGATG AGGAGTACCT GAGCCCCCCA GAGGAGTTCC CAGAGCCTGG GGAGACCTGG 
CCCCGAACCC CTACCATGAA GCTCAGTCCC AGCCAGGATC ATGATTCCTC CGACTCTTCT 
TCCAAGGCAC CCCCAACGTT CAAGGTCTCA CTCATGGACC AATCGGTGAG AGAAGGTCAA 
GATGTCATTA TGAGCATCCG TGTGCAGGGA GAGCCCAAGC CTGTGGTTTC CTGGCTGAGG 
AATCGACAGC CCGTGCGCCC AGACCAGCGG CGCTTTGCAG AGGAGGCCGA GGGTGGGCTC 
TGCCGCTTGA GGATCCTGGC TGCTGAACGG GGCGATGCTG GTTTCTACAC ATGCAAGGCG 
GTCAACGAAT ATGGCGCTCG GCAGTGCGAG GCCCGCCTGG AGGTCCGAGC ACACCCTGAA 
AGCCGGTCCC TGGCCGTGCT GGCCCCCCTG CAGGACGTGG ACGTGGGGGC CGGGGAGATG 
GCGCTGTTTG AGTGCCTGGT GGCAGGTCCA GCTGACGTCG AGGTGGACTG GCTGTGCCGG 
GGCCGCCTGC TGCAGCCTGC GCTGCTCAAA TGCAAGATGC ATTTTGATGG CCGCAAGTGC 
AAGCTGCTGC TCACCTCTGT GCACGAGGAC GACAGTGGTG TCTACACCTG CAAGCTGAGC 
ACAGCCAAAG ATGAACTGAC CTGCAGTGCC CGGCTGACGG TTCGGCCGTC GCTGGCGCCT 
CTCTTCACCC GGTTGCTGGA AGATGTGGAG GTGTTGGAAG GCCGTGCTGC CCGCTTGGAT 
TGCAAGATCA GCGGTACCCC GCCTCCCTCC GTTACCTGGA CTCATTTTGG CCACCCCGTG 
AATGAGGGTG ACAACTTGCG GCTTCAACAG GATGGAGGTC TACACTCACT GCACATTGCC 
CGGGTGGGCA GTGAGGACGA AGGACTCTAT GAAGTCAGTG CCACCAACAC TCATGGCCAG 
GCCCACTGCT CCGCCCAGCT GTATGTGGAG GAGCCTCGGA CAGCTGCCTC TGGCCCCAGC 
TCGAAGCTGG AGAAGATGCC ATCTATCCCT GAGGAGCCGG AGCACGGAGA TCTAGAGAGG 
CTGTCTATTC CTGACTTCCT ACGGCCACTG CAAGATCTGG AAGTGGGACT AGCTAAGGAG 
GCCATGCTGG AGTGCCAAGT GACCGGCCTG CCCTATCCCA CCATCAGCTG GTTCCACAAT 
GGCCACCGCA TTCAAAGCAG TGACGACCGA CGCATGACAC AGTACAGGGA CATACATCGC 
CTGGTGTTCC CAGCTGTGGG GCCTCAGCAT GCTGGTGTCT ATAAGAGTGT AATCGCCAAC 
AAGCTGGGCA AAGCGGCCTG CTACGCCCAT CTCTATGTCA CAGATGTGGT TCCAGGCCCT 
CCAGATGGTG CTCCGGAAGT AGTAGCTGTG ACCGGAAGGA TGGTCACACT ATCGTGGAAC 
CCACCCAGGA GTCTGGACAT GGCTATTGAC CCAGATTCCC TAACATACAC TGTGCAGCAC 
CAGGTGTTGG GCTCGGACCA GTGGACGGCA CTGGTCACAG GCCTGCGGGA GCCTGCGTGG 
GCAGCCACAG GGCTGAAGAA GGGGATCCAG CACATCTTCC GGGTCCTCAG CTCCAGTGGC 
AAGAGCAGCA GCAAGCCCTC AGCACCGTCG GAACCCGTGC AGCTGCTAGA GCATGGCCCA 
CCCCTGGAAG AGGCGCCTGC TGTGCTGGAC AAGCAGGACA TTGTGTATGT GGTAGAGGGC 
CAGCCTGCCT GTGTCACCGT CACTTTTAAC CATGTGGAGG CCCAGGTTGT CTGGAGGAGC 
TGCCGGGGAG CCCTCCTCGA GGCGCGCACA GGTGTGTATG AACTGAGTCA GCCAGATGAC 
GACCAGTATT GTCTGCGGAT CTGCCGGGTG AGCCGACGAG ACCTGGGACC CCTTACTTGC 
AGTGCCCGAA ACCGCCACGG CACAAAGGCC TGCTCGGTCA CCCTGGAGCT GGCAGAGGCA 
CCTCGGTTTG AGTCTATCAT GGAAGATGTG GAGGTGGGGC CTGGAGAAAC CGCTCGCTTT 
GCTGTGGTAG TTGAGGGGAA ACCACTGCCA GACATCATGT GGTACAAGGA TGAGGTGCTG 
CTGGCCGAGA GCAACCATGT GAGCTTCGTG TATGAGGAGA ACGAGTGTTC CCTAGTGCTG 
CTCAGCGCAG GAAGCCAGGA TGGAGGCGTC TATACCTGTA CCGCCCGGAA CCTGGCAGGC 
GAAGTCTCCT GCAAGGCGGA GCTGTCTGTG CTTTCAGCTC AGACAGCCAT GGAGGTCGAG 
GGGGTCGGAG AGGATGAGGA ACACCGAGGA AGAAGACTTA GCGACTACTA CGACATCCAC 
CAAGAGATTG GCAGGGGTGC CTTCTCCTAC CTGCGGCGTG TAGTGGAGAG GAGCTCTGGC 
CTGGAGTTTG CAGCCAAGTT CATCCCTAGC CAGGCTAAGC CTAAAGCATC AGCAAGACGC 
GAGGCGCGCT TGCTGGCCCG GCTCCAGCAT GGCTGTGTGC TCTACTTCCA CGAAGCCTTT 
GAGAGGCGCA GGGGACTTGT CATAGTCACG GAGCTCTGCA CAGAAGAGCT TCTAGAGCGC 
ATGGCCAGGA AGCCCACCGT GTGTGAGTCT GAGACTCGGA CCTATATGCG TCAAGTGCTA 
GAGGGGATAT GCTATCTGCA TCAGAGCCAT GTGCTGCACC TGGATGTCAA GCCGGAGAAC 
CTGCTTGTGT GGGATGGTGC AGGGGGTGAA GAGCAGGTGC GCATCTGTGA CTTCGGGAAC 
GCCCAGGAAC TCACTCCGGG AGAGCCCCAG TACTGTCAGT ATGGCACGCC AGAGTTTGTA 
GCACCTGAGA TTGTCAACCA GAGCCCTGTG TCTGGAGTCA CTGACATCTG GCCCGTGGGC 
GTCGTTGCCT TTCTCTGTTT GACGGGCATC TCCCCATTTG TTGGGGAGAA TGACCGGACA 
ACACTCATGA ATATCCGAAA CTACAACGTG GCATTTGAGG AGACTACGTT CCTGAGTCTG 
AGCAGGGAGG CCCGGGGCTT CCTCATCAAA GTGCTGGTGC AGGATCGACT GAGACCTACT 
GCAGAAGAGA CATTGGAACA TCCTTGGTTC AAAACAGAAG CAAAGGGTGC AGAGGTGAGC 
ACAGATCACC TGAAGCTCTT CCTGTCACGG AGGAGGTGGC AGCGCTCTCA AATCAGCTAC 
AAGTGCCACC TGGTGCTGCG CCCCATTCCG GAACTGCTAC GGGCTCCCCC GGAGCGGGTG 
TGGGTGGCCA TGCCTAGAAG ACAACCCCCG AGTGGGGGAC TCTCATCCTC TTCAGACTCA 
GAAGAGGAAG AGTTGGAGGA ACTTCCCTCA GTACCCCGCC CCCTGCAGCC TGAGTTCTCA 
GGTTCTCGGG TATCCCTCAC CGACATCCCC ACCGAAGATG AGGCTCTGGG GACCCCGGAA 
GCTGGGGCTG CCACCCCCAT GGACTGGCAA GAGCAGGAAA GGACTCCCTC TAAGGACCAG 
GAGGCCCCCA GCCCTGAGGC ACTCCCCTCT CCAGGCCAGG AGTCCCCTGA TGGGCCTAGC 
CCCAGGCGGC CAGAGCTCCG CAGGGGTAGC TCTGCTGAGA GTGCCCTGCC CCGAGTCGGG 
TCGCGGGAAC CGGGCCGGAG CTTGCACAAG GCAGCATCCG TGGAGCTGCC GCAGCGCAGG 
AGCCCCAGCC CGGGTGCCAC CCGCCTGACT CGGGGAGGCC TGGGTGAGGG CGAGTATGCC 
CAGAGGCTGC AGGCCCTGCG TCAGCGGCTG CTGAGGGGAG GCCCCGAGGA TGGCAAGGTC 
AGCGGCCTCA GGGGACCCCT GCTGGAGAGC CTGGGGGGCC GTGCACGGGA TCCCAGGATG 
GCAAGGGCTG CCTCCAGCGA GGCTGCCCCC CACCACCAGC CCCCTCCGGA GTCCCGGGGT 
CTGCAAAAGA GCAGCAGCTT CTCTCAGGGC GAGGCAGAGC CCCGGGGTCG GCACCGCCGC 
GCGGGGGCAC CCCTCGAAAT CCCCGTGGCC AGACTTGGGG CTCGCAGGCT GCAGGAGTCT 
CCCTCCTTAT CTGCCCTTAG CGAGACCCAG CCACCGAGCC CTGCGAGGCC CAGCGTCCCC 
AAACTCAGTA TCACCAAGTC CCCGGAACCT TCCGCTGTCA CATCCAGAGA CTCTCCCCAG 
CCCCCGGAAC CTCAGCCTGT CCCAGAAAAG GTCCCTGAGC CCAAACCAGA ACCAGTCCGA 
GCTGCAAAAC CAGCACAACC CCCTTTGGCT CTGCAAATGC CAACGCAGCC TCTCACCCCC 
TATGCCCAGA TCATGCAGTC GCTCCAGCTG TCCAGTCCCA CCCTGTCCCC ACAGGACCCC 
GCTGTGCCTC CATCAGAGCC CAAGCCCCAC GCGGCTGTGT TTGCCAGGGT GGCTTCCCCA 
CCACCCGGAG TCTCTGAGAA GCGCGTGCCT TCGGCCAGAA CTCCCCCAGT GCTAGCCGAG 
AAAGCCCGAG TCCCCACGGT GCCCCCCAGG CCAGGCAGCA GCCTCAGCGG CAGCATCGAA 
AACCTTGAAT CAGAGGCTGT GTTCGAGGCT AAGTTCAAAC GCAGCCGCGA ATCGCCACTG 
TCGCGGGGCC TGAGGCTACT GAGCCGCTCG CGCTCGGAGG AGCGCGGTCC CTTCCGTGGG 
GCGGAGGACG ACGGCATATA CCGGCCCAGT CCGGCAGGAA CGCCGCTAGA GCTGGTGCGA 
CGGCCCGAGC GCTCGCGCTC CGTGCAGGAC CTCAGGGTAG CCGGAGAGCC GGGCCTTGTG 
CGTCGCCTTT CGCTGTCCCT GTCGCAGAAG CTGCGGCGGA CACCGCCGGG ACAGCGACAC 
CCTGCCTGGG AGTCCCGCAG CGGAGACGGG GAGAGCTCCG AGGGCGGCAG CTCAGCTCGG 
GCATCCCCAG TGCTGGCGGT ACGCAGGAGA CTCAGCTCCA CGCTGGAGCG TCTGTCCAGC 
CGTTTGCAGC GCAGCGGCAG CAGCGAGGAC TCCGGGGGCG CCTCGGGTCG CAGCACGCCG 
CTGTTTGGAC GGCTGCGCAG GGCTACTTCG GAAGGGGAGA GCCTGCGACG CCTCGGTGTT 
CCGCACAATC AGCTGGGCTC TCAGACTGGT GCCACTACAC CTTCCGCTGA GTCTCTGGGC 
TCAGAGGCCA GTGGCACATC GGGTTCTTCA GCTCCTGGAG AAAGCCGAAG CCGGCACCGG 
TGGGGCCTTT CCAGGCTGCG CAAGGACAAG GGATTATCTC AGCCAAACCT CTCCTCCAGT 
GTCCAAGAGG ACTTGGGTCA CCAGTATGTG CCCAGTGAGT CAGATTTCCC CCCAGTCTTC 
CACATCAAAC TCAAGGACCA GGTGCTGTTG GAGGGGGAAG CAGCCACCCT GCTCTGCCTA 
CCCGCAGCCT GCCCAGCACC CCGCATCTCC TGGATGAAAG ATAAGCAGTC CCTGCGTTCA 
GAGCCCTCAG TGGTCATTGT GTCCTGCAAA GATGGACGGC AGCTGCTGAG CATACCCCGG 
GCCGGCAAGC GGCATGCTGG GCTCTATGAG TGCTCAGCCA CCAATGTCCT GGGCAGCATT 
ACCAGCTCCT GTACTGTGGC CGTGGCCCGC ATTCCAGGGA AGCTAGCTCC TCCAGAGGTG 
CCCCAGACCT ACCATGACAC AGCACTAGTA GTGTGGAAGC CAGGAGACGG TCGGGCACCT 
TGCACATACA CTCTGGAGCG GCGGGTGGAT GGGGAATCTG TCTGGCACCC TGTGAGCTCA 
GGCATCCCCG ACTGTTATTA CAATGTGACT CAGCTGCCTG TCGGCGTGAC CGTGCGGTTC 
CGTGTGGCCT GTTCCAATCG TGCTGGGCAG GGGCCCTTCA GCAACCCTTC TGAGAAGGTC 
TTCATCCGAG GCACTCCAGA TTCTCCTGCT CAGCCAGCTG CTGCTCCCCG AGATGCCCCT 
GTTACCTCAG GGCCAACCAG GGCCCCACCG CCTGACTCTC CTACCTCACT GGCCCCCACC 
CCCGCTCTTG CGCCCCCAGC CTCCCAGGCA TCCACTCTCA GCCCCTCAAC TTCCTCCATG 
TCTGCCAACC AGGCCTTGTC CTCACTCAAG GCTGTGGGTC CACCACCCGC AACCCCTCCA 
CGAAAGCACA GGGGCCTTCT GGCCACCCAG CAAGCAGAGC CATCCCCACC CAGTATCGTG 
GTCACCCCAA GTGAACCCAG GTCTTTTGTC CCTGACACTG GAACCCTGAC CCCAACCTCT 
AGTCCTCAAG GGGTTAAACC AGCACCTTCC TCAACTTCCT TGTATATGGT GACTTCCTTC 
GTGTCTGCAC CTCCAGCCCC CCAGGCCCCA GCCCCTGAGC CCCCTCCTGA GCCCACCAAG 
GTGACTGTGC GGAGCCTCAG CCCAGCCAAG GAGGTGGTCA GCTCCCCAAC TCCTGAGAGC 
ACCACTCTTC GGCAGGGTCC CCCTCAGAAA CCCTACACCT TCCTGGAGGA GAAGGCCAGG 
GGCCGCTTTG GCGTTGTGCG GTCATGCCGG GAGAATGCTA CGGGCCGAAC GTTTGTCGCC 
AAGATCGTGC CGTACGCCGC GGAGGGCAAG CGACGCGTGC TGCAGGAATA CGAGGTGCTT 
CGGACGCTGC ACCACGAGCG GCTCATGTCC CTGCACGAGG CCTACATCAC CCCTCGGTAC 
CTCGTGCTTA TCGCTGAGAG CTGTGGCAAC CGGGAACTCC TCTGTGGGCT CAGCGACAGA 
TTCCGGTATT CAGAAGATGA CGTGGCCACC TATGTGGTGC AGCTGCTACA AGGCCTGGAC 
TACCTCCACG GCCACCACGT GCTACACCTG GACATCAAGC CGGACAATCT ACTGTTGGCC 
GCTGACAATG CCCTTAAAAT TGTGGACTTC GGCAGTGCTC AGCCCTATAA CCCTCAGGCC 
CTGAAGCCAC TTGGCCACCG CACAGGCACT CTGGAGTTCA TGGCTCCTGA GATGGTGAAG 
GGAGACCCCA TTGGCTCTGC CACGGACATC TGGGGAGCGG GTGTGCTCAC CTACATCATG 
CTTAGTGGGT ACTCCCCATT CTATGAGCCA GACCCCCAGG AAACAGAGGC TCGGATTGTT 
GGGGGTCGCT TTGATGCCTT CCAGTTGTAT CCTAACACAT CCCAGAGTGC CACCCTCTTC 
TTGAGAAAGG TCCTCTCAGT ACATCCCTGG AGCCGGCCCT CTCTGCAGGA CTGCTTGGCC 
CACCCATGGC TGCAAGATGC CTACCTGATG AAGCTGCGCC GCCAGACACT CACCTTCACC 
ACCAACCGGC TCAAGGAATT CCTGGGCGAG CAGCGGCGAC GTCGGGCTGA GGCTGCTACC 
CGTCACAAGG TGCTGCTCCG CTCCTACCCT GGCAGCCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236946.1
CDS	107..9748
Translation	MQKARGTRGEDAGTRAPPSPGVPPKRAKVGAGRGVLVTGDGAGAPVFLRPLKNAAVCAGSDVRLRVVVSGTPQPSLSWFRDGQLLPPPAPEPSCLWLRSCGAQDAGVYSCSAQNERGQASCEAVLTVLEVRVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPSSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_011238644.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238644.1

ORF Insert Sequence:

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
TACGACTCGG AGACTGCCGA GGATGACATC AGCGATGTGC CGGGGACCCA GCGCCTGGAG 
CTTCGGGATG ACAGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGTACC 
TCCCTGGACA CACCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG 
GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC 
CCAGGCAGCC CAAGCAGCAT TCCTCCGAGT GGATTGCACA GAGAGGAGCC TGACCTTCAG 
CCTCAGCCAG CCAGTGATGC CCTTCGTCCA CGCCCTGCCC TTCCACCTCC CTCCAAATCC 
GCTTTGCTTC CCCCACCATC TCCTCGGGTG GGAAAGAGGG CTCTGCCAGG ACCTAGTACC 
CAGCCCCCAG CTACTCCCAC TTCGCCCCAT CGGCGTGCTC AGGAGCCCTC GCTGCCTGAG 
GACATCACCA CCACGGAAGA GAAGCGAGGG AAAAAGCCCA AGTCATCCGG GCCGTCGCTG 
GCGGGCACAG TGGAGTCCCG GCCCCAGACG CCACTGAGCG AGGCTTCGGG TCGCCTGTCA 
GCATTGGGCC GCTCGCCCCG GCTGGTGCGC GCGGGGTCCC GCATCCTGGA CAAGCTACAG 
TTCTTCGAAG AGCGGCGACG CAGCCTGGAG CGCAGCGACT CGCCGCCAGC GCCCCTGCGG 
CCCTGGGTGC CCCTGCGCAA GGCTCGCTCG CTGGAGCAGC CGAAGTCCGA GGGCGGTGCG 
GCGTGGGGCA CACCCGAGGC CTCGCAGGAG GAGCTGCGGT CACCTCGGGG CAGTGTGGCA 
GAGCGGCGTC GCCTGTTCCA GCAAAAGGCG GCCTCGTTGG ATGAACGCAC GCGACAACGC 
AGTGCAACCT CGGACCTCGA ACTCCGCTTC GCCCAGGAGC TGGGTCGCAT CCGCCGATCT 
ACGTCGCGGG AGGAGCTGGT GCGTTCGCAC GAGTCCCTGC GTGCCACGCT GCAGCGCGCC 
CCATCCCCTC GGGAGCCCGG CGAGCCCCCA CTCTTCTCCC GGCCTTCCAC ACCCAAGACC 
TCACGGGCTG TGAGCCCGGC TGCCACCCAG CCGCCGCCTC CTAGTGGTGC GGGCAAATCT 
GGGGACGAGC CTGGGAGGCC CCGAAGCAGA GGGCCGGTGG GCAGGACTGA ACCGGGGGAA 
GGCCCGCAGC AGGAGATCAA GCGTCGGGAC CAATTCCCGC TAACCAGGAG CAGAGCCATC 
CAGGAGTGCA GGAGCCCTGT GCCGCCCTAC ACCGCGGATC CCCCGGAGAG CAGGACAAAA 
GCCCCCTCCG GTCGCAAGCG GGAACCCCCT GCTCAAGCGG TGCGCTTTCT GCCCTGGGCC 
ACTCCGGGAG TGGAGGACTC TGTTCTGCCC CAAACCTTGG AGAAGAATAG AGCGGGACCC 
GAGGCTGAGA AGAGGCTTCG CAGAGGACCT GAGGAGGATG GCCCCTGGGG GCCCTGGGAC 
CGCAGAGGGA CCCGCAGCCA AGGCAAAGGT CGCCGTGCTC GGCCTACTTC CCCCGAGCTC 
GAGTCCTCAG ACGACTCCTA TGTGTCCGCT GGGGAAGAGC CCCTGGAGGC ACCCGTGTTT 
GAGATCCCTC TGCAGAATAT GGTGGTGGCG CCAGGAGCTG ACGTGCTACT TAAGTGTATC 
ATCACCGCCA ACCCCCCACC CCAAGTGTCC TGGAAAAAGG ATGGGTCCAT GTTGCACAGC 
GAGGGTCGTC TTCTCATCCG GGCTGAAGGT GAACGGCACA CACTGCTGCT CAGAGAGGCC 
CAGGCTGCCG ATGCTGGGAG CTACACAGCC ACTGCCACCA ACGAACTGGG CCAAGCTACC 
TGTGCTTCTT CACTGGCTGT GAGACCTGGC GGCTCCACAT CCCCTTTCAG CAGCCCCATC 
ACCTCTGATG AGGAGTACCT GAGCCCCCCA GAGGAGTTCC CAGAGCCTGG GGAGACCTGG 
CCCCGAACCC CTACCATGAA GCTCAGTCCC AGCCAGGATC ATGATTCCTC CGACTCTTCT 
TCCAAGGCAC CCCCAACGTT CAAGGTCTCA CTCATGGACC AATCGGTGAG AGAAGGTCAA 
GATGTCATTA TGAGCATCCG TGTGCAGGGA GAGCCCAAGC CTGTGGTTTC CTGGCTGAGG 
AATCGACAGC CCGTGCGCCC AGACCAGCGG CGCTTTGCAG AGGAGGCCGA GGGTGGGCTC 
TGCCGCTTGA GGATCCTGGC TGCTGAACGG GGCGATGCTG GTTTCTACAC ATGCAAGGCG 
GTCAACGAAT ATGGCGCTCG GCAGTGCGAG GCCCGCCTGG AGGTCCGAGC ACACCCTGAA 
AGCCGGTCCC TGGCCGTGCT GGCCCCCCTG CAGGACGTGG ACGTGGGGGC CGGGGAGATG 
GCGCTGTTTG AGTGCCTGGT GGCAGGTCCA GCTGACGTCG AGGTGGACTG GCTGTGCCGG 
GGCCGCCTGC TGCAGCCTGC GCTGCTCAAA TGCAAGATGC ATTTTGATGG CCGCAAGTGC 
AAGCTGCTGC TCACCTCTGT GCACGAGGAC GACAGTGGTG TCTACACCTG CAAGCTGAGC 
ACAGCCAAAG ATGAACTGAC CTGCAGTGCC CGGCTGACGG TTCGGCCGTC GCTGGCGCCT 
CTCTTCACCC GGTTGCTGGA AGATGTGGAG GTGTTGGAAG GCCGTGCTGC CCGCTTGGAT 
TGCAAGATCA GCGGTACCCC GCCTCCCTCC GTTACCTGGA CTCATTTTGG CCACCCCGTG 
AATGAGGGTG ACAACTTGCG GCTTCAACAG GATGGAGGTC TACACTCACT GCACATTGCC 
CGGGTGGGCA GTGAGGACGA AGGACTCTAT GAAGTCAGTG CCACCAACAC TCATGGCCAG 
GCCCACTGCT CCGCCCAGCT GTATGTGGAG GAGCCTCGGA CAGCTGCCTC TGGCCCCAGC 
TCGAAGCTGG AGAAGATGCC ATCTATCCCT GAGGAGCCGG AGCACGGAGA TCTAGAGAGG 
CTGTCTATTC CTGACTTCCT ACGGCCACTG CAAGATCTGG AAGTGGGACT AGCTAAGGAG 
GCCATGCTGG AGTGCCAAGT GACCGGCCTG CCCTATCCCA CCATCAGCTG GTTCCACAAT 
GGCCACCGCA TTCAAAGCAG TGACGACCGA CGCATGACAC AGTACAGGGA CATACATCGC 
CTGGTGTTCC CAGCTGTGGG GCCTCAGCAT GCTGGTGTCT ATAAGAGTGT AATCGCCAAC 
AAGCTGGGCA AAGCGGCCTG CTACGCCCAT CTCTATGTCA CAGATGTGGT TCCAGGCCCT 
CCAGATGGTG CTCCGGAAGT AGTAGCTGTG ACCGGAAGGA TGGTCACACT ATCGTGGAAC 
CCACCCAGGA GTCTGGACAT GGCTATTGAC CCAGATTCCC TAACATACAC TGTGCAGCAC 
CAGGTGTTGG GCTCGGACCA GTGGACGGCA CTGGTCACAG GCCTGCGGGA GCCTGCGTGG 
GCAGCCACAG GGCTGAAGAA GGGGATCCAG CACATCTTCC GGGTCCTCAG CTCCAGTGGC 
AAGAGCAGCA GCAAGCCCTC AGCACCGTCG GAACCCGTGC AGCTGCTAGA GCATGGCCCA 
CCCCTGGAAG AGGCGCCTGC TGTGCTGGAC AAGCAGGACA TTGTGTATGT GGTAGAGGGC 
CAGCCTGCCT GTGTCACCGT CACTTTTAAC CATGTGGAGG CCCAGGTTGT CTGGAGGAGC 
TGCCGGGGAG CCCTCCTCGA GGCGCGCACA GGTGTGTATG AACTGAGTCA GCCAGATGAC 
GACCAGTATT GTCTGCGGAT CTGCCGGGTG AGCCGACGAG ACCTGGGACC CCTTACTTGC 
AGTGCCCGAA ACCGCCACGG CACAAAGGCC TGCTCGGTCA CCCTGGAGCT GGCAGAGGCA 
CCTCGGTTTG AGTCTATCAT GGAAGATGTG GAGGTGGGGC CTGGAGAAAC CGCTCGCTTT 
GCTGTGGTAG TTGAGGGGAA ACCACTGCCA GACATCATGT GGTACAAGGA TGAGGTGCTG 
CTGGCCGAGA GCAACCATGT GAGCTTCGTG TATGAGGAGA ACGAGTGTTC CCTAGTGCTG 
CTCAGCGCAG GAAGCCAGGA TGGAGGCGTC TATACCTGTA CCGCCCGGAA CCTGGCAGGC 
GAAGTCTCCT GCAAGGCGGA GCTGTCTGTG CTTTCAGCTC AGACAGCCAT GGAGGTCGAG 
GGGGTCGGAG AGGATGAGGA ACACCGAGGA AGAAGACTTA GCGACTACTA CGACATCCAC 
CAAGAGATTG GCAGGGGTGC CTTCTCCTAC CTGCGGCGTG TAGTGGAGAG GAGCTCTGGC 
CTGGAGTTTG CAGCCAAGTT CATCCCTAGC CAGGCTAAGC CTAAAGCATC AGCAAGACGC 
GAGGCGCGCT TGCTGGCCCG GCTCCAGCAT GGCTGTGTGC TCTACTTCCA CGAAGCCTTT 
GAGAGGCGCA GGGGACTTGT CATAGTCACG GAGCTCTGCA CAGAAGAGCT TCTAGAGCGC 
ATGGCCAGGA AGCCCACCGT GTGTGAGTCT GAGACTCGGA CCTATATGCG TCAAGTGCTA 
GAGGGGATAT GCTATCTGCA TCAGAGCCAT GTGCTGCACC TGGATGTCAA GCCGGAGAAC 
CTGCTTGTGT GGGATGGTGC AGGGGGTGAA GAGCAGGTGC GCATCTGTGA CTTCGGGAAC 
GCCCAGGAAC TCACTCCGGG AGAGCCCCAG TACTGTCAGT ATGGCACGCC AGAGTTTGTA 
GCACCTGAGA TTGTCAACCA GAGCCCTGTG TCTGGAGTCA CTGACATCTG GCCCGTGGGC 
GTCGTTGCCT TTCTCTGTTT GACGGGCATC TCCCCATTTG TTGGGGAGAA TGACCGGACA 
ACACTCATGA ATATCCGAAA CTACAACGTG GCATTTGAGG AGACTACGTT CCTGAGTCTG 
AGCAGGGAGG CCCGGGGCTT CCTCATCAAA GTGCTGGTGC AGGATCGACT GAGACCTACT 
GCAGAAGAGA CATTGGAACA TCCTTGGTTC AAAACAGAAG CAAAGGGTGC AGAGGTGAGC 
ACAGATCACC TGAAGCTCTT CCTGTCACGG AGGAGGTGGC AGCGCTCTCA AATCAGCTAC 
AAGTGCCACC TGGTGCTGCG CCCCATTCCG GAACTGCTAC GGGCTCCCCC GGAGCGGGTG 
TGGGTGGCCA TGCCTAGAAG ACAACCCCCG AGTGGGGGAC TCTCATCCTC TTCAGACTCA 
GAAGAGGAAG AGTTGGAGGA ACTTCCCTCA GTACCCCGCC CCCTGCAGCC TGAGTTCTCA 
GGTTCTCGGG TATCCCTCAC CGACATCCCC ACCGAAGATG AGGCTCTGGG GACCCCGGAA 
GCTGGGGCTG CCACCCCCAT GGACTGGCAA GAGCAGGAAA GGACTCCCTC TAAGGACCAG 
GAGGCCCCCA GCCCTGAGGC ACTCCCCTCT CCAGGCCAGG AGTCCCCTGA TGGGCCTAGC 
CCCAGGCGGC CAGAGCTCCG CAGGGGTAGC TCTGCTGAGA GTGCCCTGCC CCGAGTCGGG 
TCGCGGGAAC CGGGCCGGAG CTTGCACAAG GCAGCATCCG TGGAGCTGCC GCAGCGCAGG 
AGCCCCAGCC CGGGTGCCAC CCGCCTGACT CGGGGAGGCC TGGGTGAGGG CGAGTATGCC 
CAGAGGCTGC AGGCCCTGCG TCAGCGGCTG CTGAGGGGAG GCCCCGAGGA TGGCAAGGTC 
AGCGGCCTCA GGGGACCCCT GCTGGAGAGC CTGGGGGGCC GTGCACGGGA TCCCAGGATG 
GCAAGGGCTG CCTCCAGCGA GGCTGCCCCC CACCACCAGC CCCCTCCGGA GTCCCGGGGT 
CTGCAAAAGA GCAGCAGCTT CTCTCAGGGC GAGGCAGAGC CCCGGGGTCG GCACCGCCGC 
GCGGGGGCAC CCCTCGAAAT CCCCGTGGCC AGACTTGGGG CTCGCAGGCT GCAGGAGTCT 
CCCTCCTTAT CTGCCCTTAG CGAGACCCAG CCACCGAGCC CTGCGAGGCC CAGCGTCCCC 
AAACTCAGTA TCACCAAGTC CCCGGAACCT TCCGCTGTCA CATCCAGAGA CTCTCCCCAG 
CCCCCGGAAC CTCAGCCTGT CCCAGAAAAG GTCCCTGAGC CCAAACCAGA ACCAGTCCGA 
GCTGCAAAAC CAGCACAACC CCCTTTGGCT CTGCAAATGC CAACGCAGCC TCTCACCCCC 
TATGCCCAGA TCATGCAGTC GCTCCAGCTG TCCAGTCCCA CCCTGTCCCC ACAGGACCCC 
GCTGTGCCTC CATCAGAGCC CAAGCCCCAC GCGGCTGTGT TTGCCAGGGT GGCTTCCCCA 
CCACCCGGAG TCTCTGAGAA GCGCGTGCCT TCGGCCAGAA CTCCCCCAGT GCTAGCCGAG 
AAAGCCCGAG TCCCCACGGT GCCCCCCAGG CCAGGCAGCA GCCTCAGCGG CAGCATCGAA 
AACCTTGAAT CAGAGGCTGT GTTCGAGGCT AAGTTCAAAC GCAGCCGCGA ATCGCCACTG 
TCGCGGGGCC TGAGGCTACT GAGCCGCTCG CGCTCGGAGG AGCGCGGTCC CTTCCGTGGG 
GCGGAGGACG ACGGCATATA CCGGCCCAGT CCGGCAGGAA CGCCGCTAGA GCTGGTGCGA 
CGGCCCGAGC GCTCGCGCTC CGTGCAGGAC CTCAGGGTAG CCGGAGAGCC GGGCCTTGTG 
CGTCGCCTTT CGCTGTCCCT GTCGCAGAAG CTGCGGCGGA CACCGCCGGG ACAGCGACAC 
CCTGCCTGGG AGTCCCGCAG CGGAGACGGG GAGAGCTCCG AGGGCGGCAG CTCAGCTCGG 
GCATCCCCAG TGCTGGCGGT ACGCAGGAGA CTCAGCTCCA CGCTGGAGCG TCTGTCCAGC 
CGTTTGCAGC GCAGCGGCAG CAGCGAGGAC TCCGGGGGCG CCTCGGGTCG CAGCACGCCG 
CTGTTTGGAC GGCTGCGCAG GGCTACTTCG GAAGGGGAGA GCCTGCGACG CCTCGGTGTT 
CCGCACAATC AGCTGGGCTC TCAGACTGGT GCCACTACAC CTTCCGCTGA GTCTCTGGGC 
TCAGAGGCCA GTGGCACATC GGGTTCTTCA GCTCCTGGAG AAAGCCGAAG CCGGCACCGG 
TGGGGCCTTT CCAGGCTGCG CAAGGACAAG GGATTATCTC AGCCAAACCT CTCCTCCAGT 
GTCCAAGAGG ACTTGGGTCA CCAGTATGTG CCCAGTGAGT CAGATTTCCC CCCAGTCTTC 
CACATCAAAC TCAAGGACCA GGTGCTGTTG GAGGGGGAAG CAGCCACCCT GCTCTGCCTA 
CCCGCAGCCT GCCCAGCACC CCGCATCTCC TGGATGAAAG ATAAGCAGTC CCTGCGTTCA 
GAGCCCTCAG TGGTCATTGT GTCCTGCAAA GATGGACGGC AGCTGCTGAG CATACCCCGG 
GCCGGCAAGC GGCATGCTGG GCTCTATGAG TGCTCAGCCA CCAATGTCCT GGGCAGCATT 
ACCAGCTCCT GTACTGTGGC CGTGGCCCGC ATTCCAGGGA AGCTAGCTCC TCCAGAGGTG 
CCCCAGACCT ACCATGACAC AGCACTAGTA GTGTGGAAGC CAGGAGACGG TCGGGCACCT 
TGCACATACA CTCTGGAGCG GCGGGTGGAT GGGGAATCTG TCTGGCACCC TGTGAGCTCA 
GGCATCCCCG ACTGTTATTA CAATGTGACT CAGCTGCCTG TCGGCGTGAC CGTGCGGTTC 
CGTGTGGCCT GTTCCAATCG TGCTGGGCAG GGGCCCTTCA GCAACCCTTC TGAGAAGGTC 
TTCATCCGAG GCACTCCAGA TTCTCCTGCT CAGCCAGCTG CTGCTCCCCG AGATGCCCCT 
GTTACCTCAG GGCCAACCAG GGCCCCACCG CCTGACTCTC CTACCTCACT GGCCCCCACC 
CCCGCTCTTG CGCCCCCAGC CTCCCAGGCA TCCACTCTCA GCCCCTCAAC TTCCTCCATG 
TCTGCCAACC AGGCCTTGTC CTCACTCAAG GCTGTGGGTC CACCACCCGC AACCCCTCCA 
CGAAAGCACA GGGGCCTTCT GGCCACCCAG CAAGCAGAGC CATCCCCACC CAGTATCGTG 
GTCACCCCAA GTGAACCCAG GTCTTTTGTC CCTGACACTG GAACCCTGAC CCCAACCTCT 
AGTCCTCAAG GGGTTAAACC AGCACCTTCC TCAACTTCCT TGTATATGGT GACTTCCTTC 
GTGTCTGCAC CTCCAGCCCC CCAGGCCCCA GCCCCTGAGC CCCCTCCTGA GCCCACCAAG 
GTGACTGTGC GGAGCCTCAG CCCAGCCAAG GAGGTGGTCA GCTCCCCAAC TCCTGAGAGC 
ACCACTCTTC GGCAGGGTCC CCCTCAGAAA CCCTACACCT TCCTGGAGGA GAAGGCCAGG 
GGCCGCTTTG GCGTTGTGCG GTCATGCCGG GAGAATGCTA CGGGCCGAAC GTTTGTCGCC 
AAGATCGTGC CGTACGCCGC GGAGGGCAAG CGACGCGTGC TGCAGGAATA CGAGGTGCTT 
CGGACGCTGC ACCACGAGCG GCTCATGTCC CTGCACGAGG CCTACATCAC CCCTCGGTAC 
CTCGTGCTTA TCGCTGAGAG CTGTGGCAAC CGGGAACTCC TCTGTGGGCT CAGCGACAGA 
TTCCGGTATT CAGAAGATGA CGTGGCCACC TATGTGGTGC AGCTGCTACA AGGCCTGGAC 
TACCTCCACG GCCACCACGT GCTACACCTG GACATCAAGC CGGACAATCT ACTGTTGGCC 
GCTGACAATG CCCTTAAAAT TGTGGACTTC GGCAGTGCTC AGCCCTATAA CCCTCAGGCC 
CTGAAGCCAC TTGGCCACCG CACAGGCACT CTGGAGTTCA TGGCTCCTGA GATGGTGAAG 
GGAGACCCCA TTGGCTCTGC CACGGACATC TGGGGAGCGG GTGTGCTCAC CTACATCATG 
CTTAGTGGGT ACTCCCCATT CTATGAGCCA GACCCCCAGG AAACAGAGGC TCGGATTGTT 
GGGGGTCGCT TTGATGCCTT CCAGTTGTAT CCTAACACAT CCCAGAGTGC CACCCTCTTC 
TTGAGAAAGG TCCTCTCAGT ACATCCCTGG AGCCGGCCCT CTCTGCAGGA CTGCTTGGCC 
CACCCATGGC TGCAAGATGC CTACCTGATG AAGCTGCGCC GCCAGACACT CACCTTCACC 
ACCAACCGGC TCAAGGAATT CCTGGGCGAG CAGCGGCGAC GTCGGGCTGA GGCTGCTACC 
CGTCACAAGG TGCTGCTCCG CTCCTACCCT GGCAGCCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_011238644.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236946.1 Mus musculus SPEG complex locus (Speg), transcript variant X3, mRNA.

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CloneID	OMu67145
Clone ID Related Accession (Same CDS sequence)	XM_011238644.1 , XM_011238644.2
Accession Version	XM_011238644.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238644.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
TACGACTCGG AGACTGCCGA GGATGACATC AGCGATGTGC CGGGGACCCA GCGCCTGGAG 
CTTCGGGATG ACAGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGTACC 
TCCCTGGACA CACCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG 
GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC 
CCAGGCAGCC CAAGCAGCAT TCCTCCGAGT GGATTGCACA GAGAGGAGCC TGACCTTCAG 
CCTCAGCCAG CCAGTGATGC CCTTCGTCCA CGCCCTGCCC TTCCACCTCC CTCCAAATCC 
GCTTTGCTTC CCCCACCATC TCCTCGGGTG GGAAAGAGGG CTCTGCCAGG ACCTAGTACC 
CAGCCCCCAG CTACTCCCAC TTCGCCCCAT CGGCGTGCTC AGGAGCCCTC GCTGCCTGAG 
GACATCACCA CCACGGAAGA GAAGCGAGGG AAAAAGCCCA AGTCATCCGG GCCGTCGCTG 
GCGGGCACAG TGGAGTCCCG GCCCCAGACG CCACTGAGCG AGGCTTCGGG TCGCCTGTCA 
GCATTGGGCC GCTCGCCCCG GCTGGTGCGC GCGGGGTCCC GCATCCTGGA CAAGCTACAG 
TTCTTCGAAG AGCGGCGACG CAGCCTGGAG CGCAGCGACT CGCCGCCAGC GCCCCTGCGG 
CCCTGGGTGC CCCTGCGCAA GGCTCGCTCG CTGGAGCAGC CGAAGTCCGA GGGCGGTGCG 
GCGTGGGGCA CACCCGAGGC CTCGCAGGAG GAGCTGCGGT CACCTCGGGG CAGTGTGGCA 
GAGCGGCGTC GCCTGTTCCA GCAAAAGGCG GCCTCGTTGG ATGAACGCAC GCGACAACGC 
AGTGCAACCT CGGACCTCGA ACTCCGCTTC GCCCAGGAGC TGGGTCGCAT CCGCCGATCT 
ACGTCGCGGG AGGAGCTGGT GCGTTCGCAC GAGTCCCTGC GTGCCACGCT GCAGCGCGCC 
CCATCCCCTC GGGAGCCCGG CGAGCCCCCA CTCTTCTCCC GGCCTTCCAC ACCCAAGACC 
TCACGGGCTG TGAGCCCGGC TGCCACCCAG CCGCCGCCTC CTAGTGGTGC GGGCAAATCT 
GGGGACGAGC CTGGGAGGCC CCGAAGCAGA GGGCCGGTGG GCAGGACTGA ACCGGGGGAA 
GGCCCGCAGC AGGAGATCAA GCGTCGGGAC CAATTCCCGC TAACCAGGAG CAGAGCCATC 
CAGGAGTGCA GGAGCCCTGT GCCGCCCTAC ACCGCGGATC CCCCGGAGAG CAGGACAAAA 
GCCCCCTCCG GTCGCAAGCG GGAACCCCCT GCTCAAGCGG TGCGCTTTCT GCCCTGGGCC 
ACTCCGGGAG TGGAGGACTC TGTTCTGCCC CAAACCTTGG AGAAGAATAG AGCGGGACCC 
GAGGCTGAGA AGAGGCTTCG CAGAGGACCT GAGGAGGATG GCCCCTGGGG GCCCTGGGAC 
CGCAGAGGGA CCCGCAGCCA AGGCAAAGGT CGCCGTGCTC GGCCTACTTC CCCCGAGCTC 
GAGTCCTCAG ACGACTCCTA TGTGTCCGCT GGGGAAGAGC CCCTGGAGGC ACCCGTGTTT 
GAGATCCCTC TGCAGAATAT GGTGGTGGCG CCAGGAGCTG ACGTGCTACT TAAGTGTATC 
ATCACCGCCA ACCCCCCACC CCAAGTGTCC TGGAAAAAGG ATGGGTCCAT GTTGCACAGC 
GAGGGTCGTC TTCTCATCCG GGCTGAAGGT GAACGGCACA CACTGCTGCT CAGAGAGGCC 
CAGGCTGCCG ATGCTGGGAG CTACACAGCC ACTGCCACCA ACGAACTGGG CCAAGCTACC 
TGTGCTTCTT CACTGGCTGT GAGACCTGGC GGCTCCACAT CCCCTTTCAG CAGCCCCATC 
ACCTCTGATG AGGAGTACCT GAGCCCCCCA GAGGAGTTCC CAGAGCCTGG GGAGACCTGG 
CCCCGAACCC CTACCATGAA GCTCAGTCCC AGCCAGGATC ATGATTCCTC CGACTCTTCT 
TCCAAGGCAC CCCCAACGTT CAAGGTCTCA CTCATGGACC AATCGGTGAG AGAAGGTCAA 
GATGTCATTA TGAGCATCCG TGTGCAGGGA GAGCCCAAGC CTGTGGTTTC CTGGCTGAGG 
AATCGACAGC CCGTGCGCCC AGACCAGCGG CGCTTTGCAG AGGAGGCCGA GGGTGGGCTC 
TGCCGCTTGA GGATCCTGGC TGCTGAACGG GGCGATGCTG GTTTCTACAC ATGCAAGGCG 
GTCAACGAAT ATGGCGCTCG GCAGTGCGAG GCCCGCCTGG AGGTCCGAGC ACACCCTGAA 
AGCCGGTCCC TGGCCGTGCT GGCCCCCCTG CAGGACGTGG ACGTGGGGGC CGGGGAGATG 
GCGCTGTTTG AGTGCCTGGT GGCAGGTCCA GCTGACGTCG AGGTGGACTG GCTGTGCCGG 
GGCCGCCTGC TGCAGCCTGC GCTGCTCAAA TGCAAGATGC ATTTTGATGG CCGCAAGTGC 
AAGCTGCTGC TCACCTCTGT GCACGAGGAC GACAGTGGTG TCTACACCTG CAAGCTGAGC 
ACAGCCAAAG ATGAACTGAC CTGCAGTGCC CGGCTGACGG TTCGGCCGTC GCTGGCGCCT 
CTCTTCACCC GGTTGCTGGA AGATGTGGAG GTGTTGGAAG GCCGTGCTGC CCGCTTGGAT 
TGCAAGATCA GCGGTACCCC GCCTCCCTCC GTTACCTGGA CTCATTTTGG CCACCCCGTG 
AATGAGGGTG ACAACTTGCG GCTTCAACAG GATGGAGGTC TACACTCACT GCACATTGCC 
CGGGTGGGCA GTGAGGACGA AGGACTCTAT GAAGTCAGTG CCACCAACAC TCATGGCCAG 
GCCCACTGCT CCGCCCAGCT GTATGTGGAG GAGCCTCGGA CAGCTGCCTC TGGCCCCAGC 
TCGAAGCTGG AGAAGATGCC ATCTATCCCT GAGGAGCCGG AGCACGGAGA TCTAGAGAGG 
CTGTCTATTC CTGACTTCCT ACGGCCACTG CAAGATCTGG AAGTGGGACT AGCTAAGGAG 
GCCATGCTGG AGTGCCAAGT GACCGGCCTG CCCTATCCCA CCATCAGCTG GTTCCACAAT 
GGCCACCGCA TTCAAAGCAG TGACGACCGA CGCATGACAC AGTACAGGGA CATACATCGC 
CTGGTGTTCC CAGCTGTGGG GCCTCAGCAT GCTGGTGTCT ATAAGAGTGT AATCGCCAAC 
AAGCTGGGCA AAGCGGCCTG CTACGCCCAT CTCTATGTCA CAGATGTGGT TCCAGGCCCT 
CCAGATGGTG CTCCGGAAGT AGTAGCTGTG ACCGGAAGGA TGGTCACACT ATCGTGGAAC 
CCACCCAGGA GTCTGGACAT GGCTATTGAC CCAGATTCCC TAACATACAC TGTGCAGCAC 
CAGGTGTTGG GCTCGGACCA GTGGACGGCA CTGGTCACAG GCCTGCGGGA GCCTGCGTGG 
GCAGCCACAG GGCTGAAGAA GGGGATCCAG CACATCTTCC GGGTCCTCAG CTCCAGTGGC 
AAGAGCAGCA GCAAGCCCTC AGCACCGTCG GAACCCGTGC AGCTGCTAGA GCATGGCCCA 
CCCCTGGAAG AGGCGCCTGC TGTGCTGGAC AAGCAGGACA TTGTGTATGT GGTAGAGGGC 
CAGCCTGCCT GTGTCACCGT CACTTTTAAC CATGTGGAGG CCCAGGTTGT CTGGAGGAGC 
TGCCGGGGAG CCCTCCTCGA GGCGCGCACA GGTGTGTATG AACTGAGTCA GCCAGATGAC 
GACCAGTATT GTCTGCGGAT CTGCCGGGTG AGCCGACGAG ACCTGGGACC CCTTACTTGC 
AGTGCCCGAA ACCGCCACGG CACAAAGGCC TGCTCGGTCA CCCTGGAGCT GGCAGAGGCA 
CCTCGGTTTG AGTCTATCAT GGAAGATGTG GAGGTGGGGC CTGGAGAAAC CGCTCGCTTT 
GCTGTGGTAG TTGAGGGGAA ACCACTGCCA GACATCATGT GGTACAAGGA TGAGGTGCTG 
CTGGCCGAGA GCAACCATGT GAGCTTCGTG TATGAGGAGA ACGAGTGTTC CCTAGTGCTG 
CTCAGCGCAG GAAGCCAGGA TGGAGGCGTC TATACCTGTA CCGCCCGGAA CCTGGCAGGC 
GAAGTCTCCT GCAAGGCGGA GCTGTCTGTG CTTTCAGCTC AGACAGCCAT GGAGGTCGAG 
GGGGTCGGAG AGGATGAGGA ACACCGAGGA AGAAGACTTA GCGACTACTA CGACATCCAC 
CAAGAGATTG GCAGGGGTGC CTTCTCCTAC CTGCGGCGTG TAGTGGAGAG GAGCTCTGGC 
CTGGAGTTTG CAGCCAAGTT CATCCCTAGC CAGGCTAAGC CTAAAGCATC AGCAAGACGC 
GAGGCGCGCT TGCTGGCCCG GCTCCAGCAT GGCTGTGTGC TCTACTTCCA CGAAGCCTTT 
GAGAGGCGCA GGGGACTTGT CATAGTCACG GAGCTCTGCA CAGAAGAGCT TCTAGAGCGC 
ATGGCCAGGA AGCCCACCGT GTGTGAGTCT GAGACTCGGA CCTATATGCG TCAAGTGCTA 
GAGGGGATAT GCTATCTGCA TCAGAGCCAT GTGCTGCACC TGGATGTCAA GCCGGAGAAC 
CTGCTTGTGT GGGATGGTGC AGGGGGTGAA GAGCAGGTGC GCATCTGTGA CTTCGGGAAC 
GCCCAGGAAC TCACTCCGGG AGAGCCCCAG TACTGTCAGT ATGGCACGCC AGAGTTTGTA 
GCACCTGAGA TTGTCAACCA GAGCCCTGTG TCTGGAGTCA CTGACATCTG GCCCGTGGGC 
GTCGTTGCCT TTCTCTGTTT GACGGGCATC TCCCCATTTG TTGGGGAGAA TGACCGGACA 
ACACTCATGA ATATCCGAAA CTACAACGTG GCATTTGAGG AGACTACGTT CCTGAGTCTG 
AGCAGGGAGG CCCGGGGCTT CCTCATCAAA GTGCTGGTGC AGGATCGACT GAGACCTACT 
GCAGAAGAGA CATTGGAACA TCCTTGGTTC AAAACAGAAG CAAAGGGTGC AGAGGTGAGC 
ACAGATCACC TGAAGCTCTT CCTGTCACGG AGGAGGTGGC AGCGCTCTCA AATCAGCTAC 
AAGTGCCACC TGGTGCTGCG CCCCATTCCG GAACTGCTAC GGGCTCCCCC GGAGCGGGTG 
TGGGTGGCCA TGCCTAGAAG ACAACCCCCG AGTGGGGGAC TCTCATCCTC TTCAGACTCA 
GAAGAGGAAG AGTTGGAGGA ACTTCCCTCA GTACCCCGCC CCCTGCAGCC TGAGTTCTCA 
GGTTCTCGGG TATCCCTCAC CGACATCCCC ACCGAAGATG AGGCTCTGGG GACCCCGGAA 
GCTGGGGCTG CCACCCCCAT GGACTGGCAA GAGCAGGAAA GGACTCCCTC TAAGGACCAG 
GAGGCCCCCA GCCCTGAGGC ACTCCCCTCT CCAGGCCAGG AGTCCCCTGA TGGGCCTAGC 
CCCAGGCGGC CAGAGCTCCG CAGGGGTAGC TCTGCTGAGA GTGCCCTGCC CCGAGTCGGG 
TCGCGGGAAC CGGGCCGGAG CTTGCACAAG GCAGCATCCG TGGAGCTGCC GCAGCGCAGG 
AGCCCCAGCC CGGGTGCCAC CCGCCTGACT CGGGGAGGCC TGGGTGAGGG CGAGTATGCC 
CAGAGGCTGC AGGCCCTGCG TCAGCGGCTG CTGAGGGGAG GCCCCGAGGA TGGCAAGGTC 
AGCGGCCTCA GGGGACCCCT GCTGGAGAGC CTGGGGGGCC GTGCACGGGA TCCCAGGATG 
GCAAGGGCTG CCTCCAGCGA GGCTGCCCCC CACCACCAGC CCCCTCCGGA GTCCCGGGGT 
CTGCAAAAGA GCAGCAGCTT CTCTCAGGGC GAGGCAGAGC CCCGGGGTCG GCACCGCCGC 
GCGGGGGCAC CCCTCGAAAT CCCCGTGGCC AGACTTGGGG CTCGCAGGCT GCAGGAGTCT 
CCCTCCTTAT CTGCCCTTAG CGAGACCCAG CCACCGAGCC CTGCGAGGCC CAGCGTCCCC 
AAACTCAGTA TCACCAAGTC CCCGGAACCT TCCGCTGTCA CATCCAGAGA CTCTCCCCAG 
CCCCCGGAAC CTCAGCCTGT CCCAGAAAAG GTCCCTGAGC CCAAACCAGA ACCAGTCCGA 
GCTGCAAAAC CAGCACAACC CCCTTTGGCT CTGCAAATGC CAACGCAGCC TCTCACCCCC 
TATGCCCAGA TCATGCAGTC GCTCCAGCTG TCCAGTCCCA CCCTGTCCCC ACAGGACCCC 
GCTGTGCCTC CATCAGAGCC CAAGCCCCAC GCGGCTGTGT TTGCCAGGGT GGCTTCCCCA 
CCACCCGGAG TCTCTGAGAA GCGCGTGCCT TCGGCCAGAA CTCCCCCAGT GCTAGCCGAG 
AAAGCCCGAG TCCCCACGGT GCCCCCCAGG CCAGGCAGCA GCCTCAGCGG CAGCATCGAA 
AACCTTGAAT CAGAGGCTGT GTTCGAGGCT AAGTTCAAAC GCAGCCGCGA ATCGCCACTG 
TCGCGGGGCC TGAGGCTACT GAGCCGCTCG CGCTCGGAGG AGCGCGGTCC CTTCCGTGGG 
GCGGAGGACG ACGGCATATA CCGGCCCAGT CCGGCAGGAA CGCCGCTAGA GCTGGTGCGA 
CGGCCCGAGC GCTCGCGCTC CGTGCAGGAC CTCAGGGTAG CCGGAGAGCC GGGCCTTGTG 
CGTCGCCTTT CGCTGTCCCT GTCGCAGAAG CTGCGGCGGA CACCGCCGGG ACAGCGACAC 
CCTGCCTGGG AGTCCCGCAG CGGAGACGGG GAGAGCTCCG AGGGCGGCAG CTCAGCTCGG 
GCATCCCCAG TGCTGGCGGT ACGCAGGAGA CTCAGCTCCA CGCTGGAGCG TCTGTCCAGC 
CGTTTGCAGC GCAGCGGCAG CAGCGAGGAC TCCGGGGGCG CCTCGGGTCG CAGCACGCCG 
CTGTTTGGAC GGCTGCGCAG GGCTACTTCG GAAGGGGAGA GCCTGCGACG CCTCGGTGTT 
CCGCACAATC AGCTGGGCTC TCAGACTGGT GCCACTACAC CTTCCGCTGA GTCTCTGGGC 
TCAGAGGCCA GTGGCACATC GGGTTCTTCA GCTCCTGGAG AAAGCCGAAG CCGGCACCGG 
TGGGGCCTTT CCAGGCTGCG CAAGGACAAG GGATTATCTC AGCCAAACCT CTCCTCCAGT 
GTCCAAGAGG ACTTGGGTCA CCAGTATGTG CCCAGTGAGT CAGATTTCCC CCCAGTCTTC 
CACATCAAAC TCAAGGACCA GGTGCTGTTG GAGGGGGAAG CAGCCACCCT GCTCTGCCTA 
CCCGCAGCCT GCCCAGCACC CCGCATCTCC TGGATGAAAG ATAAGCAGTC CCTGCGTTCA 
GAGCCCTCAG TGGTCATTGT GTCCTGCAAA GATGGACGGC AGCTGCTGAG CATACCCCGG 
GCCGGCAAGC GGCATGCTGG GCTCTATGAG TGCTCAGCCA CCAATGTCCT GGGCAGCATT 
ACCAGCTCCT GTACTGTGGC CGTGGCCCGC ATTCCAGGGA AGCTAGCTCC TCCAGAGGTG 
CCCCAGACCT ACCATGACAC AGCACTAGTA GTGTGGAAGC CAGGAGACGG TCGGGCACCT 
TGCACATACA CTCTGGAGCG GCGGGTGGAT GGGGAATCTG TCTGGCACCC TGTGAGCTCA 
GGCATCCCCG ACTGTTATTA CAATGTGACT CAGCTGCCTG TCGGCGTGAC CGTGCGGTTC 
CGTGTGGCCT GTTCCAATCG TGCTGGGCAG GGGCCCTTCA GCAACCCTTC TGAGAAGGTC 
TTCATCCGAG GCACTCCAGA TTCTCCTGCT CAGCCAGCTG CTGCTCCCCG AGATGCCCCT 
GTTACCTCAG GGCCAACCAG GGCCCCACCG CCTGACTCTC CTACCTCACT GGCCCCCACC 
CCCGCTCTTG CGCCCCCAGC CTCCCAGGCA TCCACTCTCA GCCCCTCAAC TTCCTCCATG 
TCTGCCAACC AGGCCTTGTC CTCACTCAAG GCTGTGGGTC CACCACCCGC AACCCCTCCA 
CGAAAGCACA GGGGCCTTCT GGCCACCCAG CAAGCAGAGC CATCCCCACC CAGTATCGTG 
GTCACCCCAA GTGAACCCAG GTCTTTTGTC CCTGACACTG GAACCCTGAC CCCAACCTCT 
AGTCCTCAAG GGGTTAAACC AGCACCTTCC TCAACTTCCT TGTATATGGT GACTTCCTTC 
GTGTCTGCAC CTCCAGCCCC CCAGGCCCCA GCCCCTGAGC CCCCTCCTGA GCCCACCAAG 
GTGACTGTGC GGAGCCTCAG CCCAGCCAAG GAGGTGGTCA GCTCCCCAAC TCCTGAGAGC 
ACCACTCTTC GGCAGGGTCC CCCTCAGAAA CCCTACACCT TCCTGGAGGA GAAGGCCAGG 
GGCCGCTTTG GCGTTGTGCG GTCATGCCGG GAGAATGCTA CGGGCCGAAC GTTTGTCGCC 
AAGATCGTGC CGTACGCCGC GGAGGGCAAG CGACGCGTGC TGCAGGAATA CGAGGTGCTT 
CGGACGCTGC ACCACGAGCG GCTCATGTCC CTGCACGAGG CCTACATCAC CCCTCGGTAC 
CTCGTGCTTA TCGCTGAGAG CTGTGGCAAC CGGGAACTCC TCTGTGGGCT CAGCGACAGA 
TTCCGGTATT CAGAAGATGA CGTGGCCACC TATGTGGTGC AGCTGCTACA AGGCCTGGAC 
TACCTCCACG GCCACCACGT GCTACACCTG GACATCAAGC CGGACAATCT ACTGTTGGCC 
GCTGACAATG CCCTTAAAAT TGTGGACTTC GGCAGTGCTC AGCCCTATAA CCCTCAGGCC 
CTGAAGCCAC TTGGCCACCG CACAGGCACT CTGGAGTTCA TGGCTCCTGA GATGGTGAAG 
GGAGACCCCA TTGGCTCTGC CACGGACATC TGGGGAGCGG GTGTGCTCAC CTACATCATG 
CTTAGTGGGT ACTCCCCATT CTATGAGCCA GACCCCCAGG AAACAGAGGC TCGGATTGTT 
GGGGGTCGCT TTGATGCCTT CCAGTTGTAT CCTAACACAT CCCAGAGTGC CACCCTCTTC 
TTGAGAAAGG TCCTCTCAGT ACATCCCTGG AGCCGGCCCT CTCTGCAGGA CTGCTTGGCC 
CACCCATGGC TGCAAGATGC CTACCTGATG AAGCTGCGCC GCCAGACACT CACCTTCACC 
ACCAACCGGC TCAAGGAATT CCTGGGCGAG CAGCGGCGAC GTCGGGCTGA GGCTGCTACC 
CGTCACAAGG TGCTGCTCCG CTCCTACCCT GGCAGCCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236946.1
CDS	103..9744
Translation	MQKARGTRGEDAGTRAPPSPGVPPKRAKVGAGRGVLVTGDGAGAPVFLRPLKNAAVCAGSDVRLRVVVSGTPQPSLSWFRDGQLLPPPAPEPSCLWLRSCGAQDAGVYSCSAQNERGQASCEAVLTVLEVRVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPSSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_011238644.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238644.2

ORF Insert Sequence:

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
TACGACTCGG AGACTGCCGA GGATGACATC AGCGATGTGC CGGGGACCCA GCGCCTGGAG 
CTTCGGGATG ACAGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGTACC 
TCCCTGGACA CACCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG 
GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC 
CCAGGCAGCC CAAGCAGCAT TCCTCCGAGT GGATTGCACA GAGAGGAGCC TGACCTTCAG 
CCTCAGCCAG CCAGTGATGC CCTTCGTCCA CGCCCTGCCC TTCCACCTCC CTCCAAATCC 
GCTTTGCTTC CCCCACCATC TCCTCGGGTG GGAAAGAGGG CTCTGCCAGG ACCTAGTACC 
CAGCCCCCAG CTACTCCCAC TTCGCCCCAT CGGCGTGCTC AGGAGCCCTC GCTGCCTGAG 
GACATCACCA CCACGGAAGA GAAGCGAGGG AAAAAGCCCA AGTCATCCGG GCCGTCGCTG 
GCGGGCACAG TGGAGTCCCG GCCCCAGACG CCACTGAGCG AGGCTTCGGG TCGCCTGTCA 
GCATTGGGCC GCTCGCCCCG GCTGGTGCGC GCGGGGTCCC GCATCCTGGA CAAGCTACAG 
TTCTTCGAAG AGCGGCGACG CAGCCTGGAG CGCAGCGACT CGCCGCCAGC GCCCCTGCGG 
CCCTGGGTGC CCCTGCGCAA GGCTCGCTCG CTGGAGCAGC CGAAGTCCGA GGGCGGTGCG 
GCGTGGGGCA CACCCGAGGC CTCGCAGGAG GAGCTGCGGT CACCTCGGGG CAGTGTGGCA 
GAGCGGCGTC GCCTGTTCCA GCAAAAGGCG GCCTCGTTGG ATGAACGCAC GCGACAACGC 
AGTGCAACCT CGGACCTCGA ACTCCGCTTC GCCCAGGAGC TGGGTCGCAT CCGCCGATCT 
ACGTCGCGGG AGGAGCTGGT GCGTTCGCAC GAGTCCCTGC GTGCCACGCT GCAGCGCGCC 
CCATCCCCTC GGGAGCCCGG CGAGCCCCCA CTCTTCTCCC GGCCTTCCAC ACCCAAGACC 
TCACGGGCTG TGAGCCCGGC TGCCACCCAG CCGCCGCCTC CTAGTGGTGC GGGCAAATCT 
GGGGACGAGC CTGGGAGGCC CCGAAGCAGA GGGCCGGTGG GCAGGACTGA ACCGGGGGAA 
GGCCCGCAGC AGGAGATCAA GCGTCGGGAC CAATTCCCGC TAACCAGGAG CAGAGCCATC 
CAGGAGTGCA GGAGCCCTGT GCCGCCCTAC ACCGCGGATC CCCCGGAGAG CAGGACAAAA 
GCCCCCTCCG GTCGCAAGCG GGAACCCCCT GCTCAAGCGG TGCGCTTTCT GCCCTGGGCC 
ACTCCGGGAG TGGAGGACTC TGTTCTGCCC CAAACCTTGG AGAAGAATAG AGCGGGACCC 
GAGGCTGAGA AGAGGCTTCG CAGAGGACCT GAGGAGGATG GCCCCTGGGG GCCCTGGGAC 
CGCAGAGGGA CCCGCAGCCA AGGCAAAGGT CGCCGTGCTC GGCCTACTTC CCCCGAGCTC 
GAGTCCTCAG ACGACTCCTA TGTGTCCGCT GGGGAAGAGC CCCTGGAGGC ACCCGTGTTT 
GAGATCCCTC TGCAGAATAT GGTGGTGGCG CCAGGAGCTG ACGTGCTACT TAAGTGTATC 
ATCACCGCCA ACCCCCCACC CCAAGTGTCC TGGAAAAAGG ATGGGTCCAT GTTGCACAGC 
GAGGGTCGTC TTCTCATCCG GGCTGAAGGT GAACGGCACA CACTGCTGCT CAGAGAGGCC 
CAGGCTGCCG ATGCTGGGAG CTACACAGCC ACTGCCACCA ACGAACTGGG CCAAGCTACC 
TGTGCTTCTT CACTGGCTGT GAGACCTGGC GGCTCCACAT CCCCTTTCAG CAGCCCCATC 
ACCTCTGATG AGGAGTACCT GAGCCCCCCA GAGGAGTTCC CAGAGCCTGG GGAGACCTGG 
CCCCGAACCC CTACCATGAA GCTCAGTCCC AGCCAGGATC ATGATTCCTC CGACTCTTCT 
TCCAAGGCAC CCCCAACGTT CAAGGTCTCA CTCATGGACC AATCGGTGAG AGAAGGTCAA 
GATGTCATTA TGAGCATCCG TGTGCAGGGA GAGCCCAAGC CTGTGGTTTC CTGGCTGAGG 
AATCGACAGC CCGTGCGCCC AGACCAGCGG CGCTTTGCAG AGGAGGCCGA GGGTGGGCTC 
TGCCGCTTGA GGATCCTGGC TGCTGAACGG GGCGATGCTG GTTTCTACAC ATGCAAGGCG 
GTCAACGAAT ATGGCGCTCG GCAGTGCGAG GCCCGCCTGG AGGTCCGAGC ACACCCTGAA 
AGCCGGTCCC TGGCCGTGCT GGCCCCCCTG CAGGACGTGG ACGTGGGGGC CGGGGAGATG 
GCGCTGTTTG AGTGCCTGGT GGCAGGTCCA GCTGACGTCG AGGTGGACTG GCTGTGCCGG 
GGCCGCCTGC TGCAGCCTGC GCTGCTCAAA TGCAAGATGC ATTTTGATGG CCGCAAGTGC 
AAGCTGCTGC TCACCTCTGT GCACGAGGAC GACAGTGGTG TCTACACCTG CAAGCTGAGC 
ACAGCCAAAG ATGAACTGAC CTGCAGTGCC CGGCTGACGG TTCGGCCGTC GCTGGCGCCT 
CTCTTCACCC GGTTGCTGGA AGATGTGGAG GTGTTGGAAG GCCGTGCTGC CCGCTTGGAT 
TGCAAGATCA GCGGTACCCC GCCTCCCTCC GTTACCTGGA CTCATTTTGG CCACCCCGTG 
AATGAGGGTG ACAACTTGCG GCTTCAACAG GATGGAGGTC TACACTCACT GCACATTGCC 
CGGGTGGGCA GTGAGGACGA AGGACTCTAT GAAGTCAGTG CCACCAACAC TCATGGCCAG 
GCCCACTGCT CCGCCCAGCT GTATGTGGAG GAGCCTCGGA CAGCTGCCTC TGGCCCCAGC 
TCGAAGCTGG AGAAGATGCC ATCTATCCCT GAGGAGCCGG AGCACGGAGA TCTAGAGAGG 
CTGTCTATTC CTGACTTCCT ACGGCCACTG CAAGATCTGG AAGTGGGACT AGCTAAGGAG 
GCCATGCTGG AGTGCCAAGT GACCGGCCTG CCCTATCCCA CCATCAGCTG GTTCCACAAT 
GGCCACCGCA TTCAAAGCAG TGACGACCGA CGCATGACAC AGTACAGGGA CATACATCGC 
CTGGTGTTCC CAGCTGTGGG GCCTCAGCAT GCTGGTGTCT ATAAGAGTGT AATCGCCAAC 
AAGCTGGGCA AAGCGGCCTG CTACGCCCAT CTCTATGTCA CAGATGTGGT TCCAGGCCCT 
CCAGATGGTG CTCCGGAAGT AGTAGCTGTG ACCGGAAGGA TGGTCACACT ATCGTGGAAC 
CCACCCAGGA GTCTGGACAT GGCTATTGAC CCAGATTCCC TAACATACAC TGTGCAGCAC 
CAGGTGTTGG GCTCGGACCA GTGGACGGCA CTGGTCACAG GCCTGCGGGA GCCTGCGTGG 
GCAGCCACAG GGCTGAAGAA GGGGATCCAG CACATCTTCC GGGTCCTCAG CTCCAGTGGC 
AAGAGCAGCA GCAAGCCCTC AGCACCGTCG GAACCCGTGC AGCTGCTAGA GCATGGCCCA 
CCCCTGGAAG AGGCGCCTGC TGTGCTGGAC AAGCAGGACA TTGTGTATGT GGTAGAGGGC 
CAGCCTGCCT GTGTCACCGT CACTTTTAAC CATGTGGAGG CCCAGGTTGT CTGGAGGAGC 
TGCCGGGGAG CCCTCCTCGA GGCGCGCACA GGTGTGTATG AACTGAGTCA GCCAGATGAC 
GACCAGTATT GTCTGCGGAT CTGCCGGGTG AGCCGACGAG ACCTGGGACC CCTTACTTGC 
AGTGCCCGAA ACCGCCACGG CACAAAGGCC TGCTCGGTCA CCCTGGAGCT GGCAGAGGCA 
CCTCGGTTTG AGTCTATCAT GGAAGATGTG GAGGTGGGGC CTGGAGAAAC CGCTCGCTTT 
GCTGTGGTAG TTGAGGGGAA ACCACTGCCA GACATCATGT GGTACAAGGA TGAGGTGCTG 
CTGGCCGAGA GCAACCATGT GAGCTTCGTG TATGAGGAGA ACGAGTGTTC CCTAGTGCTG 
CTCAGCGCAG GAAGCCAGGA TGGAGGCGTC TATACCTGTA CCGCCCGGAA CCTGGCAGGC 
GAAGTCTCCT GCAAGGCGGA GCTGTCTGTG CTTTCAGCTC AGACAGCCAT GGAGGTCGAG 
GGGGTCGGAG AGGATGAGGA ACACCGAGGA AGAAGACTTA GCGACTACTA CGACATCCAC 
CAAGAGATTG GCAGGGGTGC CTTCTCCTAC CTGCGGCGTG TAGTGGAGAG GAGCTCTGGC 
CTGGAGTTTG CAGCCAAGTT CATCCCTAGC CAGGCTAAGC CTAAAGCATC AGCAAGACGC 
GAGGCGCGCT TGCTGGCCCG GCTCCAGCAT GGCTGTGTGC TCTACTTCCA CGAAGCCTTT 
GAGAGGCGCA GGGGACTTGT CATAGTCACG GAGCTCTGCA CAGAAGAGCT TCTAGAGCGC 
ATGGCCAGGA AGCCCACCGT GTGTGAGTCT GAGACTCGGA CCTATATGCG TCAAGTGCTA 
GAGGGGATAT GCTATCTGCA TCAGAGCCAT GTGCTGCACC TGGATGTCAA GCCGGAGAAC 
CTGCTTGTGT GGGATGGTGC AGGGGGTGAA GAGCAGGTGC GCATCTGTGA CTTCGGGAAC 
GCCCAGGAAC TCACTCCGGG AGAGCCCCAG TACTGTCAGT ATGGCACGCC AGAGTTTGTA 
GCACCTGAGA TTGTCAACCA GAGCCCTGTG TCTGGAGTCA CTGACATCTG GCCCGTGGGC 
GTCGTTGCCT TTCTCTGTTT GACGGGCATC TCCCCATTTG TTGGGGAGAA TGACCGGACA 
ACACTCATGA ATATCCGAAA CTACAACGTG GCATTTGAGG AGACTACGTT CCTGAGTCTG 
AGCAGGGAGG CCCGGGGCTT CCTCATCAAA GTGCTGGTGC AGGATCGACT GAGACCTACT 
GCAGAAGAGA CATTGGAACA TCCTTGGTTC AAAACAGAAG CAAAGGGTGC AGAGGTGAGC 
ACAGATCACC TGAAGCTCTT CCTGTCACGG AGGAGGTGGC AGCGCTCTCA AATCAGCTAC 
AAGTGCCACC TGGTGCTGCG CCCCATTCCG GAACTGCTAC GGGCTCCCCC GGAGCGGGTG 
TGGGTGGCCA TGCCTAGAAG ACAACCCCCG AGTGGGGGAC TCTCATCCTC TTCAGACTCA 
GAAGAGGAAG AGTTGGAGGA ACTTCCCTCA GTACCCCGCC CCCTGCAGCC TGAGTTCTCA 
GGTTCTCGGG TATCCCTCAC CGACATCCCC ACCGAAGATG AGGCTCTGGG GACCCCGGAA 
GCTGGGGCTG CCACCCCCAT GGACTGGCAA GAGCAGGAAA GGACTCCCTC TAAGGACCAG 
GAGGCCCCCA GCCCTGAGGC ACTCCCCTCT CCAGGCCAGG AGTCCCCTGA TGGGCCTAGC 
CCCAGGCGGC CAGAGCTCCG CAGGGGTAGC TCTGCTGAGA GTGCCCTGCC CCGAGTCGGG 
TCGCGGGAAC CGGGCCGGAG CTTGCACAAG GCAGCATCCG TGGAGCTGCC GCAGCGCAGG 
AGCCCCAGCC CGGGTGCCAC CCGCCTGACT CGGGGAGGCC TGGGTGAGGG CGAGTATGCC 
CAGAGGCTGC AGGCCCTGCG TCAGCGGCTG CTGAGGGGAG GCCCCGAGGA TGGCAAGGTC 
AGCGGCCTCA GGGGACCCCT GCTGGAGAGC CTGGGGGGCC GTGCACGGGA TCCCAGGATG 
GCAAGGGCTG CCTCCAGCGA GGCTGCCCCC CACCACCAGC CCCCTCCGGA GTCCCGGGGT 
CTGCAAAAGA GCAGCAGCTT CTCTCAGGGC GAGGCAGAGC CCCGGGGTCG GCACCGCCGC 
GCGGGGGCAC CCCTCGAAAT CCCCGTGGCC AGACTTGGGG CTCGCAGGCT GCAGGAGTCT 
CCCTCCTTAT CTGCCCTTAG CGAGACCCAG CCACCGAGCC CTGCGAGGCC CAGCGTCCCC 
AAACTCAGTA TCACCAAGTC CCCGGAACCT TCCGCTGTCA CATCCAGAGA CTCTCCCCAG 
CCCCCGGAAC CTCAGCCTGT CCCAGAAAAG GTCCCTGAGC CCAAACCAGA ACCAGTCCGA 
GCTGCAAAAC CAGCACAACC CCCTTTGGCT CTGCAAATGC CAACGCAGCC TCTCACCCCC 
TATGCCCAGA TCATGCAGTC GCTCCAGCTG TCCAGTCCCA CCCTGTCCCC ACAGGACCCC 
GCTGTGCCTC CATCAGAGCC CAAGCCCCAC GCGGCTGTGT TTGCCAGGGT GGCTTCCCCA 
CCACCCGGAG TCTCTGAGAA GCGCGTGCCT TCGGCCAGAA CTCCCCCAGT GCTAGCCGAG 
AAAGCCCGAG TCCCCACGGT GCCCCCCAGG CCAGGCAGCA GCCTCAGCGG CAGCATCGAA 
AACCTTGAAT CAGAGGCTGT GTTCGAGGCT AAGTTCAAAC GCAGCCGCGA ATCGCCACTG 
TCGCGGGGCC TGAGGCTACT GAGCCGCTCG CGCTCGGAGG AGCGCGGTCC CTTCCGTGGG 
GCGGAGGACG ACGGCATATA CCGGCCCAGT CCGGCAGGAA CGCCGCTAGA GCTGGTGCGA 
CGGCCCGAGC GCTCGCGCTC CGTGCAGGAC CTCAGGGTAG CCGGAGAGCC GGGCCTTGTG 
CGTCGCCTTT CGCTGTCCCT GTCGCAGAAG CTGCGGCGGA CACCGCCGGG ACAGCGACAC 
CCTGCCTGGG AGTCCCGCAG CGGAGACGGG GAGAGCTCCG AGGGCGGCAG CTCAGCTCGG 
GCATCCCCAG TGCTGGCGGT ACGCAGGAGA CTCAGCTCCA CGCTGGAGCG TCTGTCCAGC 
CGTTTGCAGC GCAGCGGCAG CAGCGAGGAC TCCGGGGGCG CCTCGGGTCG CAGCACGCCG 
CTGTTTGGAC GGCTGCGCAG GGCTACTTCG GAAGGGGAGA GCCTGCGACG CCTCGGTGTT 
CCGCACAATC AGCTGGGCTC TCAGACTGGT GCCACTACAC CTTCCGCTGA GTCTCTGGGC 
TCAGAGGCCA GTGGCACATC GGGTTCTTCA GCTCCTGGAG AAAGCCGAAG CCGGCACCGG 
TGGGGCCTTT CCAGGCTGCG CAAGGACAAG GGATTATCTC AGCCAAACCT CTCCTCCAGT 
GTCCAAGAGG ACTTGGGTCA CCAGTATGTG CCCAGTGAGT CAGATTTCCC CCCAGTCTTC 
CACATCAAAC TCAAGGACCA GGTGCTGTTG GAGGGGGAAG CAGCCACCCT GCTCTGCCTA 
CCCGCAGCCT GCCCAGCACC CCGCATCTCC TGGATGAAAG ATAAGCAGTC CCTGCGTTCA 
GAGCCCTCAG TGGTCATTGT GTCCTGCAAA GATGGACGGC AGCTGCTGAG CATACCCCGG 
GCCGGCAAGC GGCATGCTGG GCTCTATGAG TGCTCAGCCA CCAATGTCCT GGGCAGCATT 
ACCAGCTCCT GTACTGTGGC CGTGGCCCGC ATTCCAGGGA AGCTAGCTCC TCCAGAGGTG 
CCCCAGACCT ACCATGACAC AGCACTAGTA GTGTGGAAGC CAGGAGACGG TCGGGCACCT 
TGCACATACA CTCTGGAGCG GCGGGTGGAT GGGGAATCTG TCTGGCACCC TGTGAGCTCA 
GGCATCCCCG ACTGTTATTA CAATGTGACT CAGCTGCCTG TCGGCGTGAC CGTGCGGTTC 
CGTGTGGCCT GTTCCAATCG TGCTGGGCAG GGGCCCTTCA GCAACCCTTC TGAGAAGGTC 
TTCATCCGAG GCACTCCAGA TTCTCCTGCT CAGCCAGCTG CTGCTCCCCG AGATGCCCCT 
GTTACCTCAG GGCCAACCAG GGCCCCACCG CCTGACTCTC CTACCTCACT GGCCCCCACC 
CCCGCTCTTG CGCCCCCAGC CTCCCAGGCA TCCACTCTCA GCCCCTCAAC TTCCTCCATG 
TCTGCCAACC AGGCCTTGTC CTCACTCAAG GCTGTGGGTC CACCACCCGC AACCCCTCCA 
CGAAAGCACA GGGGCCTTCT GGCCACCCAG CAAGCAGAGC CATCCCCACC CAGTATCGTG 
GTCACCCCAA GTGAACCCAG GTCTTTTGTC CCTGACACTG GAACCCTGAC CCCAACCTCT 
AGTCCTCAAG GGGTTAAACC AGCACCTTCC TCAACTTCCT TGTATATGGT GACTTCCTTC 
GTGTCTGCAC CTCCAGCCCC CCAGGCCCCA GCCCCTGAGC CCCCTCCTGA GCCCACCAAG 
GTGACTGTGC GGAGCCTCAG CCCAGCCAAG GAGGTGGTCA GCTCCCCAAC TCCTGAGAGC 
ACCACTCTTC GGCAGGGTCC CCCTCAGAAA CCCTACACCT TCCTGGAGGA GAAGGCCAGG 
GGCCGCTTTG GCGTTGTGCG GTCATGCCGG GAGAATGCTA CGGGCCGAAC GTTTGTCGCC 
AAGATCGTGC CGTACGCCGC GGAGGGCAAG CGACGCGTGC TGCAGGAATA CGAGGTGCTT 
CGGACGCTGC ACCACGAGCG GCTCATGTCC CTGCACGAGG CCTACATCAC CCCTCGGTAC 
CTCGTGCTTA TCGCTGAGAG CTGTGGCAAC CGGGAACTCC TCTGTGGGCT CAGCGACAGA 
TTCCGGTATT CAGAAGATGA CGTGGCCACC TATGTGGTGC AGCTGCTACA AGGCCTGGAC 
TACCTCCACG GCCACCACGT GCTACACCTG GACATCAAGC CGGACAATCT ACTGTTGGCC 
GCTGACAATG CCCTTAAAAT TGTGGACTTC GGCAGTGCTC AGCCCTATAA CCCTCAGGCC 
CTGAAGCCAC TTGGCCACCG CACAGGCACT CTGGAGTTCA TGGCTCCTGA GATGGTGAAG 
GGAGACCCCA TTGGCTCTGC CACGGACATC TGGGGAGCGG GTGTGCTCAC CTACATCATG 
CTTAGTGGGT ACTCCCCATT CTATGAGCCA GACCCCCAGG AAACAGAGGC TCGGATTGTT 
GGGGGTCGCT TTGATGCCTT CCAGTTGTAT CCTAACACAT CCCAGAGTGC CACCCTCTTC 
TTGAGAAAGG TCCTCTCAGT ACATCCCTGG AGCCGGCCCT CTCTGCAGGA CTGCTTGGCC 
CACCCATGGC TGCAAGATGC CTACCTGATG AAGCTGCGCC GCCAGACACT CACCTTCACC 
ACCAACCGGC TCAAGGAATT CCTGGGCGAG CAGCGGCGAC GTCGGGCTGA GGCTGCTACC 
CGTCACAAGG TGCTGCTCCG CTCCTACCCT GGCAGCCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_011238645.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236947.1 Mus musculus SPEG complex locus (Speg), transcript variant X4, mRNA.

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CloneID	OMu67146
Clone ID Related Accession (Same CDS sequence)	XM_011238645.1 , XM_011238645.2
Accession Version	XM_011238645.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA 
GCACAGACAA CCGGGGCCGG GCCACGGCAC CTGGGGGTGG AGCCGCTGGT GCGGGCATCG 
CGAGCTAATC TGGTGGGCGC AAGCTGGGGG TCAGAGGATA GCCTTTCGGT GGCCAGTGAC 
CTCTATGGCA GCGCGTTCAG TCTGTACAGA GGACGGGCGC TCTCGATCCA CGTCAGCATT 
CCTCCGAGTG GATTGCACAG AGAGGAGCCT GACCTTCAGC CTCAGCCAGC CAGTGATGCC 
CTTCGTCCAC GCCCTGCCCT TCCACCTCCC TCCAAATCCG CTTTGCTTCC CCCACCATCT 
CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA CCTAGTACCC AGCCCCCAGC TACTCCCACT 
TCGCCCCATC GGCGTGCTCA GGAGCCCTCG CTGCCTGAGG ACATCACCAC CACGGAAGAG 
AAGCGAGGGA AAAAGCCCAA GTCATCCGGG CCGTCGCTGG CGGGCACAGT GGAGTCCCGG 
CCCCAGACGC CACTGAGCGA GGCTTCGGGT CGCCTGTCAG CATTGGGCCG CTCGCCCCGG 
CTGGTGCGCG CGGGGTCCCG CATCCTGGAC AAGCTACAGT TCTTCGAAGA GCGGCGACGC 
AGCCTGGAGC GCAGCGACTC GCCGCCAGCG CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG 
GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC 
TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG 
CAAAAGGCGG CCTCGTTGGA TGAACGCACG CGACAACGCA GTGCAACCTC GGACCTCGAA 
CTCCGCTTCG CCCAGGAGCT GGGTCGCATC CGCCGATCTA CGTCGCGGGA GGAGCTGGTG 
CGTTCGCACG AGTCCCTGCG TGCCACGCTG CAGCGCGCCC CATCCCCTCG GGAGCCCGGC 
GAGCCCCCAC TCTTCTCCCG GCCTTCCACA CCCAAGACCT CACGGGCTGT GAGCCCGGCT 
GCCACCCAGC CGCCGCCTCC TAGTGGTGCG GGCAAATCTG GGGACGAGCC TGGGAGGCCC 
CGAAGCAGAG GGCCGGTGGG CAGGACTGAA CCGGGGGAAG GCCCGCAGCA GGAGATCAAG 
CGTCGGGACC AATTCCCGCT AACCAGGAGC AGAGCCATCC AGGAGTGCAG GAGCCCTGTG 
CCGCCCTACA CCGCGGATCC CCCGGAGAGC AGGACAAAAG CCCCCTCCGG TCGCAAGCGG 
GAACCCCCTG CTCAAGCGGT GCGCTTTCTG CCCTGGGCCA CTCCGGGAGT GGAGGACTCT 
GTTCTGCCCC AAACCTTGGA GAAGAATAGA GCGGGACCCG AGGCTGAGAA GAGGCTTCGC 
AGAGGACCTG AGGAGGATGG CCCCTGGGGG CCCTGGGACC GCAGAGGGAC CCGCAGCCAA 
GGCAAAGGTC GCCGTGCTCG GCCTACTTCC CCCGAGCTCG AGTCCTCAGA CGACTCCTAT 
GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA CCCGTGTTTG AGATCCCTCT GCAGAATATG 
GTGGTGGCGC CAGGAGCTGA CGTGCTACTT AAGTGTATCA TCACCGCCAA CCCCCCACCC 
CAAGTGTCCT GGAAAAAGGA TGGGTCCATG TTGCACAGCG AGGGTCGTCT TCTCATCCGG 
GCTGAAGGTG AACGGCACAC ACTGCTGCTC AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC 
TACACAGCCA CTGCCACCAA CGAACTGGGC CAAGCTACCT GTGCTTCTTC ACTGGCTGTG 
AGACCTGGCG GCTCCACATC CCCTTTCAGC AGCCCCATCA CCTCTGATGA GGAGTACCTG 
AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG GAGACCTGGC CCCGAACCCC TACCATGAAG 
CTCAGTCCCA GCCAGGATCA TGATTCCTCC GACTCTTCTT CCAAGGCACC CCCAACGTTC 
AAGGTCTCAC TCATGGACCA ATCGGTGAGA GAAGGTCAAG ATGTCATTAT GAGCATCCGT 
GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC TGGCTGAGGA ATCGACAGCC CGTGCGCCCA 
GACCAGCGGC GCTTTGCAGA GGAGGCCGAG GGTGGGCTCT GCCGCTTGAG GATCCTGGCT 
GCTGAACGGG GCGATGCTGG TTTCTACACA TGCAAGGCGG TCAACGAATA TGGCGCTCGG 
CAGTGCGAGG CCCGCCTGGA GGTCCGAGCA CACCCTGAAA GCCGGTCCCT GGCCGTGCTG 
GCCCCCCTGC AGGACGTGGA CGTGGGGGCC GGGGAGATGG CGCTGTTTGA GTGCCTGGTG 
GCAGGTCCAG CTGACGTCGA GGTGGACTGG CTGTGCCGGG GCCGCCTGCT GCAGCCTGCG 
CTGCTCAAAT GCAAGATGCA TTTTGATGGC CGCAAGTGCA AGCTGCTGCT CACCTCTGTG 
CACGAGGACG ACAGTGGTGT CTACACCTGC AAGCTGAGCA CAGCCAAAGA TGAACTGACC 
TGCAGTGCCC GGCTGACGGT TCGGCCGTCG CTGGCGCCTC TCTTCACCCG GTTGCTGGAA 
GATGTGGAGG TGTTGGAAGG CCGTGCTGCC CGCTTGGATT GCAAGATCAG CGGTACCCCG 
CCTCCCTCCG TTACCTGGAC TCATTTTGGC CACCCCGTGA ATGAGGGTGA CAACTTGCGG 
CTTCAACAGG ATGGAGGTCT ACACTCACTG CACATTGCCC GGGTGGGCAG TGAGGACGAA 
GGACTCTATG AAGTCAGTGC CACCAACACT CATGGCCAGG CCCACTGCTC CGCCCAGCTG 
TATGTGGAGG AGCCTCGGAC AGCTGCCTCT GGCCCCAGCT CGAAGCTGGA GAAGATGCCA 
TCTATCCCTG AGGAGCCGGA GCACGGAGAT CTAGAGAGGC TGTCTATTCC TGACTTCCTA 
CGGCCACTGC AAGATCTGGA AGTGGGACTA GCTAAGGAGG CCATGCTGGA GTGCCAAGTG 
ACCGGCCTGC CCTATCCCAC CATCAGCTGG TTCCACAATG GCCACCGCAT TCAAAGCAGT 
GACGACCGAC GCATGACACA GTACAGGGAC ATACATCGCC TGGTGTTCCC AGCTGTGGGG 
CCTCAGCATG CTGGTGTCTA TAAGAGTGTA ATCGCCAACA AGCTGGGCAA AGCGGCCTGC 
TACGCCCATC TCTATGTCAC AGATGTGGTT CCAGGCCCTC CAGATGGTGC TCCGGAAGTA 
GTAGCTGTGA CCGGAAGGAT GGTCACACTA TCGTGGAACC CACCCAGGAG TCTGGACATG 
GCTATTGACC CAGATTCCCT AACATACACT GTGCAGCACC AGGTGTTGGG CTCGGACCAG 
TGGACGGCAC TGGTCACAGG CCTGCGGGAG CCTGCGTGGG CAGCCACAGG GCTGAAGAAG 
GGGATCCAGC ACATCTTCCG GGTCCTCAGC TCCAGTGGCA AGAGCAGCAG CAAGCCCTCA 
GCACCGTCGG AACCCGTGCA GCTGCTAGAG CATGGCCCAC CCCTGGAAGA GGCGCCTGCT 
GTGCTGGACA AGCAGGACAT TGTGTATGTG GTAGAGGGCC AGCCTGCCTG TGTCACCGTC 
ACTTTTAACC ATGTGGAGGC CCAGGTTGTC TGGAGGAGCT GCCGGGGAGC CCTCCTCGAG 
GCGCGCACAG GTGTGTATGA ACTGAGTCAG CCAGATGACG ACCAGTATTG TCTGCGGATC 
TGCCGGGTGA GCCGACGAGA CCTGGGACCC CTTACTTGCA GTGCCCGAAA CCGCCACGGC 
ACAAAGGCCT GCTCGGTCAC CCTGGAGCTG GCAGAGGCAC CTCGGTTTGA GTCTATCATG 
GAAGATGTGG AGGTGGGGCC TGGAGAAACC GCTCGCTTTG CTGTGGTAGT TGAGGGGAAA 
CCACTGCCAG ACATCATGTG GTACAAGGAT GAGGTGCTGC TGGCCGAGAG CAACCATGTG 
AGCTTCGTGT ATGAGGAGAA CGAGTGTTCC CTAGTGCTGC TCAGCGCAGG AAGCCAGGAT 
GGAGGCGTCT ATACCTGTAC CGCCCGGAAC CTGGCAGGCG AAGTCTCCTG CAAGGCGGAG 
CTGTCTGTGC TTTCAGCTCA GACAGCCATG GAGGTCGAGG GGGTCGGAGA GGATGAGGAA 
CACCGAGGAA GAAGACTTAG CGACTACTAC GACATCCACC AAGAGATTGG CAGGGGTGCC 
TTCTCCTACC TGCGGCGTGT AGTGGAGAGG AGCTCTGGCC TGGAGTTTGC AGCCAAGTTC 
ATCCCTAGCC AGGCTAAGCC TAAAGCATCA GCAAGACGCG AGGCGCGCTT GCTGGCCCGG 
CTCCAGCATG GCTGTGTGCT CTACTTCCAC GAAGCCTTTG AGAGGCGCAG GGGACTTGTC 
ATAGTCACGG AGCTCTGCAC AGAAGAGCTT CTAGAGCGCA TGGCCAGGAA GCCCACCGTG 
TGTGAGTCTG AGACTCGGAC CTATATGCGT CAAGTGCTAG AGGGGATATG CTATCTGCAT 
CAGAGCCATG TGCTGCACCT GGATGTCAAG CCGGAGAACC TGCTTGTGTG GGATGGTGCA 
GGGGGTGAAG AGCAGGTGCG CATCTGTGAC TTCGGGAACG CCCAGGAACT CACTCCGGGA 
GAGCCCCAGT ACTGTCAGTA TGGCACGCCA GAGTTTGTAG CACCTGAGAT TGTCAACCAG 
AGCCCTGTGT CTGGAGTCAC TGACATCTGG CCCGTGGGCG TCGTTGCCTT TCTCTGTTTG 
ACGGGCATCT CCCCATTTGT TGGGGAGAAT GACCGGACAA CACTCATGAA TATCCGAAAC 
TACAACGTGG CATTTGAGGA GACTACGTTC CTGAGTCTGA GCAGGGAGGC CCGGGGCTTC 
CTCATCAAAG TGCTGGTGCA GGATCGACTG AGACCTACTG CAGAAGAGAC ATTGGAACAT 
CCTTGGTTCA AAACAGAAGC AAAGGGTGCA GAGGTGAGCA CAGATCACCT GAAGCTCTTC 
CTGTCACGGA GGAGGTGGCA GCGCTCTCAA ATCAGCTACA AGTGCCACCT GGTGCTGCGC 
CCCATTCCGG AACTGCTACG GGCTCCCCCG GAGCGGGTGT GGGTGGCCAT GCCTAGAAGA 
CAACCCCCGA GTGGGGGACT CTCATCCTCT TCAGACTCAG AAGAGGAAGA GTTGGAGGAA 
CTTCCCTCAG TACCCCGCCC CCTGCAGCCT GAGTTCTCAG GTTCTCGGGT ATCCCTCACC 
GACATCCCCA CCGAAGATGA GGCTCTGGGG ACCCCGGAAG CTGGGGCTGC CACCCCCATG 
GACTGGCAAG AGCAGGAAAG GACTCCCTCT AAGGACCAGG AGGCCCCCAG CCCTGAGGCA 
CTCCCCTCTC CAGGCCAGGA GTCCCCTGAT GGGCCTAGCC CCAGGCGGCC AGAGCTCCGC 
AGGGGTAGCT CTGCTGAGAG TGCCCTGCCC CGAGTCGGGT CGCGGGAACC GGGCCGGAGC 
TTGCACAAGG CAGCATCCGT GGAGCTGCCG CAGCGCAGGA GCCCCAGCCC GGGTGCCACC 
CGCCTGACTC GGGGAGGCCT GGGTGAGGGC GAGTATGCCC AGAGGCTGCA GGCCCTGCGT 
CAGCGGCTGC TGAGGGGAGG CCCCGAGGAT GGCAAGGTCA GCGGCCTCAG GGGACCCCTG 
CTGGAGAGCC TGGGGGGCCG TGCACGGGAT CCCAGGATGG CAAGGGCTGC CTCCAGCGAG 
GCTGCCCCCC ACCACCAGCC CCCTCCGGAG TCCCGGGGTC TGCAAAAGAG CAGCAGCTTC 
TCTCAGGGCG AGGCAGAGCC CCGGGGTCGG CACCGCCGCG CGGGGGCACC CCTCGAAATC 
CCCGTGGCCA GACTTGGGGC TCGCAGGCTG CAGGAGTCTC CCTCCTTATC TGCCCTTAGC 
GAGACCCAGC CACCGAGCCC TGCGAGGCCC AGCGTCCCCA AACTCAGTAT CACCAAGTCC 
CCGGAACCTT CCGCTGTCAC ATCCAGAGAC TCTCCCCAGC CCCCGGAACC TCAGCCTGTC 
CCAGAAAAGG TCCCTGAGCC CAAACCAGAA CCAGTCCGAG CTGCAAAACC AGCACAACCC 
CCTTTGGCTC TGCAAATGCC AACGCAGCCT CTCACCCCCT ATGCCCAGAT CATGCAGTCG 
CTCCAGCTGT CCAGTCCCAC CCTGTCCCCA CAGGACCCCG CTGTGCCTCC ATCAGAGCCC 
AAGCCCCACG CGGCTGTGTT TGCCAGGGTG GCTTCCCCAC CACCCGGAGT CTCTGAGAAG 
CGCGTGCCTT CGGCCAGAAC TCCCCCAGTG CTAGCCGAGA AAGCCCGAGT CCCCACGGTG 
CCCCCCAGGC CAGGCAGCAG CCTCAGCGGC AGCATCGAAA ACCTTGAATC AGAGGCTGTG 
TTCGAGGCTA AGTTCAAACG CAGCCGCGAA TCGCCACTGT CGCGGGGCCT GAGGCTACTG 
AGCCGCTCGC GCTCGGAGGA GCGCGGTCCC TTCCGTGGGG CGGAGGACGA CGGCATATAC 
CGGCCCAGTC CGGCAGGAAC GCCGCTAGAG CTGGTGCGAC GGCCCGAGCG CTCGCGCTCC 
GTGCAGGACC TCAGGGTAGC CGGAGAGCCG GGCCTTGTGC GTCGCCTTTC GCTGTCCCTG 
TCGCAGAAGC TGCGGCGGAC ACCGCCGGGA CAGCGACACC CTGCCTGGGA GTCCCGCAGC 
GGAGACGGGG AGAGCTCCGA GGGCGGCAGC TCAGCTCGGG CATCCCCAGT GCTGGCGGTA 
CGCAGGAGAC TCAGCTCCAC GCTGGAGCGT CTGTCCAGCC GTTTGCAGCG CAGCGGCAGC 
AGCGAGGACT CCGGGGGCGC CTCGGGTCGC AGCACGCCGC TGTTTGGACG GCTGCGCAGG 
GCTACTTCGG AAGGGGAGAG CCTGCGACGC CTCGGTGTTC CGCACAATCA GCTGGGCTCT 
CAGACTGGTG CCACTACACC TTCCGCTGAG TCTCTGGGCT CAGAGGCCAG TGGCACATCG 
GGTTCTTCAG CTCCTGGAGA AAGCCGAAGC CGGCACCGGT GGGGCCTTTC CAGGCTGCGC 
AAGGACAAGG GATTATCTCA GCCAAACCTC TCCTCCAGTG TCCAAGAGGA CTTGGGTCAC 
CAGTATGTGC CCAGTGAGTC AGATTTCCCC CCAGTCTTCC ACATCAAACT CAAGGACCAG 
GTGCTGTTGG AGGGGGAAGC AGCCACCCTG CTCTGCCTAC CCGCAGCCTG CCCAGCACCC 
CGCATCTCCT GGATGAAAGA TAAGCAGTCC CTGCGTTCAG AGCCCTCAGT GGTCATTGTG 
TCCTGCAAAG ATGGACGGCA GCTGCTGAGC ATACCCCGGG CCGGCAAGCG GCATGCTGGG 
CTCTATGAGT GCTCAGCCAC CAATGTCCTG GGCAGCATTA CCAGCTCCTG TACTGTGGCC 
GTGGCCCGCA TTCCAGGGAA GCTAGCTCCT CCAGAGGTGC CCCAGACCTA CCATGACACA 
GCACTAGTAG TGTGGAAGCC AGGAGACGGT CGGGCACCTT GCACATACAC TCTGGAGCGG 
CGGGTGGATG GGGAATCTGT CTGGCACCCT GTGAGCTCAG GCATCCCCGA CTGTTATTAC 
AATGTGACTC AGCTGCCTGT CGGCGTGACC GTGCGGTTCC GTGTGGCCTG TTCCAATCGT 
GCTGGGCAGG GGCCCTTCAG CAACCCTTCT GAGAAGGTCT TCATCCGAGG CACTCCAGAT 
TCTCCTGCTC AGCCAGCTGC TGCTCCCCGA GATGCCCCTG TTACCTCAGG GCCAACCAGG 
GCCCCACCGC CTGACTCTCC TACCTCACTG GCCCCCACCC CCGCTCTTGC GCCCCCAGCC 
TCCCAGGCAT CCACTCTCAG CCCCTCAACT TCCTCCATGT CTGCCAACCA GGCCTTGTCC 
TCACTCAAGG CTGTGGGTCC ACCACCCGCA ACCCCTCCAC GAAAGCACAG GGGCCTTCTG 
GCCACCCAGC AAGCAGAGCC ATCCCCACCC AGTATCGTGG TCACCCCAAG TGAACCCAGG 
TCTTTTGTCC CTGACACTGG AACCCTGACC CCAACCTCTA GTCCTCAAGG GGTTAAACCA 
GCACCTTCCT CAACTTCCTT GTATATGGTG ACTTCCTTCG TGTCTGCACC TCCAGCCCCC 
CAGGCCCCAG CCCCTGAGCC CCCTCCTGAG CCCACCAAGG TGACTGTGCG GAGCCTCAGC 
CCAGCCAAGG AGGTGGTCAG CTCCCCAACT CCTGAGAGCA CCACTCTTCG GCAGGGTCCC 
CCTCAGAAAC CCTACACCTT CCTGGAGGAG AAGGCCAGGG GCCGCTTTGG CGTTGTGCGG 
TCATGCCGGG AGAATGCTAC GGGCCGAACG TTTGTCGCCA AGATCGTGCC GTACGCCGCG 
GAGGGCAAGC GACGCGTGCT GCAGGAATAC GAGGTGCTTC GGACGCTGCA CCACGAGCGG 
CTCATGTCCC TGCACGAGGC CTACATCACC CCTCGGTACC TCGTGCTTAT CGCTGAGAGC 
TGTGGCAACC GGGAACTCCT CTGTGGGCTC AGCGACAGAT TCCGGTATTC AGAAGATGAC 
GTGGCCACCT ATGTGGTGCA GCTGCTACAA GGCCTGGACT ACCTCCACGG CCACCACGTG 
CTACACCTGG ACATCAAGCC GGACAATCTA CTGTTGGCCG CTGACAATGC CCTTAAAATT 
GTGGACTTCG GCAGTGCTCA GCCCTATAAC CCTCAGGCCC TGAAGCCACT TGGCCACCGC 
ACAGGCACTC TGGAGTTCAT GGCTCCTGAG ATGGTGAAGG GAGACCCCAT TGGCTCTGCC 
ACGGACATCT GGGGAGCGGG TGTGCTCACC TACATCATGC TTAGTGGGTA CTCCCCATTC 
TATGAGCCAG ACCCCCAGGA AACAGAGGCT CGGATTGTTG GGGGTCGCTT TGATGCCTTC 
CAGTTGTATC CTAACACATC CCAGAGTGCC ACCCTCTTCT TGAGAAAGGT CCTCTCAGTA 
CATCCCTGGA GCCGGCCCTC TCTGCAGGAC TGCTTGGCCC ACCCATGGCT GCAAGATGCC 
TACCTGATGA AGCTGCGCCG CCAGACACTC ACCTTCACCA CCAACCGGCT CAAGGAATTC 
CTGGGCGAGC AGCGGCGACG TCGGGCTGAG GCTGCTACCC GTCACAAGGT GCTGCTCCGC 
TCCTACCCTG GCAGCCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236947.1
CDS	65..9565
Translation	MKKLWVKKRFQKTGHSRRAFGRLTHVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPRQAQTTGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_011238645.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238645.1

ORF Insert Sequence:

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA 
GCACAGACAA CCGGGGCCGG GCCACGGCAC CTGGGGGTGG AGCCGCTGGT GCGGGCATCG 
CGAGCTAATC TGGTGGGCGC AAGCTGGGGG TCAGAGGATA GCCTTTCGGT GGCCAGTGAC 
CTCTATGGCA GCGCGTTCAG TCTGTACAGA GGACGGGCGC TCTCGATCCA CGTCAGCATT 
CCTCCGAGTG GATTGCACAG AGAGGAGCCT GACCTTCAGC CTCAGCCAGC CAGTGATGCC 
CTTCGTCCAC GCCCTGCCCT TCCACCTCCC TCCAAATCCG CTTTGCTTCC CCCACCATCT 
CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA CCTAGTACCC AGCCCCCAGC TACTCCCACT 
TCGCCCCATC GGCGTGCTCA GGAGCCCTCG CTGCCTGAGG ACATCACCAC CACGGAAGAG 
AAGCGAGGGA AAAAGCCCAA GTCATCCGGG CCGTCGCTGG CGGGCACAGT GGAGTCCCGG 
CCCCAGACGC CACTGAGCGA GGCTTCGGGT CGCCTGTCAG CATTGGGCCG CTCGCCCCGG 
CTGGTGCGCG CGGGGTCCCG CATCCTGGAC AAGCTACAGT TCTTCGAAGA GCGGCGACGC 
AGCCTGGAGC GCAGCGACTC GCCGCCAGCG CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG 
GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC 
TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG 
CAAAAGGCGG CCTCGTTGGA TGAACGCACG CGACAACGCA GTGCAACCTC GGACCTCGAA 
CTCCGCTTCG CCCAGGAGCT GGGTCGCATC CGCCGATCTA CGTCGCGGGA GGAGCTGGTG 
CGTTCGCACG AGTCCCTGCG TGCCACGCTG CAGCGCGCCC CATCCCCTCG GGAGCCCGGC 
GAGCCCCCAC TCTTCTCCCG GCCTTCCACA CCCAAGACCT CACGGGCTGT GAGCCCGGCT 
GCCACCCAGC CGCCGCCTCC TAGTGGTGCG GGCAAATCTG GGGACGAGCC TGGGAGGCCC 
CGAAGCAGAG GGCCGGTGGG CAGGACTGAA CCGGGGGAAG GCCCGCAGCA GGAGATCAAG 
CGTCGGGACC AATTCCCGCT AACCAGGAGC AGAGCCATCC AGGAGTGCAG GAGCCCTGTG 
CCGCCCTACA CCGCGGATCC CCCGGAGAGC AGGACAAAAG CCCCCTCCGG TCGCAAGCGG 
GAACCCCCTG CTCAAGCGGT GCGCTTTCTG CCCTGGGCCA CTCCGGGAGT GGAGGACTCT 
GTTCTGCCCC AAACCTTGGA GAAGAATAGA GCGGGACCCG AGGCTGAGAA GAGGCTTCGC 
AGAGGACCTG AGGAGGATGG CCCCTGGGGG CCCTGGGACC GCAGAGGGAC CCGCAGCCAA 
GGCAAAGGTC GCCGTGCTCG GCCTACTTCC CCCGAGCTCG AGTCCTCAGA CGACTCCTAT 
GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA CCCGTGTTTG AGATCCCTCT GCAGAATATG 
GTGGTGGCGC CAGGAGCTGA CGTGCTACTT AAGTGTATCA TCACCGCCAA CCCCCCACCC 
CAAGTGTCCT GGAAAAAGGA TGGGTCCATG TTGCACAGCG AGGGTCGTCT TCTCATCCGG 
GCTGAAGGTG AACGGCACAC ACTGCTGCTC AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC 
TACACAGCCA CTGCCACCAA CGAACTGGGC CAAGCTACCT GTGCTTCTTC ACTGGCTGTG 
AGACCTGGCG GCTCCACATC CCCTTTCAGC AGCCCCATCA CCTCTGATGA GGAGTACCTG 
AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG GAGACCTGGC CCCGAACCCC TACCATGAAG 
CTCAGTCCCA GCCAGGATCA TGATTCCTCC GACTCTTCTT CCAAGGCACC CCCAACGTTC 
AAGGTCTCAC TCATGGACCA ATCGGTGAGA GAAGGTCAAG ATGTCATTAT GAGCATCCGT 
GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC TGGCTGAGGA ATCGACAGCC CGTGCGCCCA 
GACCAGCGGC GCTTTGCAGA GGAGGCCGAG GGTGGGCTCT GCCGCTTGAG GATCCTGGCT 
GCTGAACGGG GCGATGCTGG TTTCTACACA TGCAAGGCGG TCAACGAATA TGGCGCTCGG 
CAGTGCGAGG CCCGCCTGGA GGTCCGAGCA CACCCTGAAA GCCGGTCCCT GGCCGTGCTG 
GCCCCCCTGC AGGACGTGGA CGTGGGGGCC GGGGAGATGG CGCTGTTTGA GTGCCTGGTG 
GCAGGTCCAG CTGACGTCGA GGTGGACTGG CTGTGCCGGG GCCGCCTGCT GCAGCCTGCG 
CTGCTCAAAT GCAAGATGCA TTTTGATGGC CGCAAGTGCA AGCTGCTGCT CACCTCTGTG 
CACGAGGACG ACAGTGGTGT CTACACCTGC AAGCTGAGCA CAGCCAAAGA TGAACTGACC 
TGCAGTGCCC GGCTGACGGT TCGGCCGTCG CTGGCGCCTC TCTTCACCCG GTTGCTGGAA 
GATGTGGAGG TGTTGGAAGG CCGTGCTGCC CGCTTGGATT GCAAGATCAG CGGTACCCCG 
CCTCCCTCCG TTACCTGGAC TCATTTTGGC CACCCCGTGA ATGAGGGTGA CAACTTGCGG 
CTTCAACAGG ATGGAGGTCT ACACTCACTG CACATTGCCC GGGTGGGCAG TGAGGACGAA 
GGACTCTATG AAGTCAGTGC CACCAACACT CATGGCCAGG CCCACTGCTC CGCCCAGCTG 
TATGTGGAGG AGCCTCGGAC AGCTGCCTCT GGCCCCAGCT CGAAGCTGGA GAAGATGCCA 
TCTATCCCTG AGGAGCCGGA GCACGGAGAT CTAGAGAGGC TGTCTATTCC TGACTTCCTA 
CGGCCACTGC AAGATCTGGA AGTGGGACTA GCTAAGGAGG CCATGCTGGA GTGCCAAGTG 
ACCGGCCTGC CCTATCCCAC CATCAGCTGG TTCCACAATG GCCACCGCAT TCAAAGCAGT 
GACGACCGAC GCATGACACA GTACAGGGAC ATACATCGCC TGGTGTTCCC AGCTGTGGGG 
CCTCAGCATG CTGGTGTCTA TAAGAGTGTA ATCGCCAACA AGCTGGGCAA AGCGGCCTGC 
TACGCCCATC TCTATGTCAC AGATGTGGTT CCAGGCCCTC CAGATGGTGC TCCGGAAGTA 
GTAGCTGTGA CCGGAAGGAT GGTCACACTA TCGTGGAACC CACCCAGGAG TCTGGACATG 
GCTATTGACC CAGATTCCCT AACATACACT GTGCAGCACC AGGTGTTGGG CTCGGACCAG 
TGGACGGCAC TGGTCACAGG CCTGCGGGAG CCTGCGTGGG CAGCCACAGG GCTGAAGAAG 
GGGATCCAGC ACATCTTCCG GGTCCTCAGC TCCAGTGGCA AGAGCAGCAG CAAGCCCTCA 
GCACCGTCGG AACCCGTGCA GCTGCTAGAG CATGGCCCAC CCCTGGAAGA GGCGCCTGCT 
GTGCTGGACA AGCAGGACAT TGTGTATGTG GTAGAGGGCC AGCCTGCCTG TGTCACCGTC 
ACTTTTAACC ATGTGGAGGC CCAGGTTGTC TGGAGGAGCT GCCGGGGAGC CCTCCTCGAG 
GCGCGCACAG GTGTGTATGA ACTGAGTCAG CCAGATGACG ACCAGTATTG TCTGCGGATC 
TGCCGGGTGA GCCGACGAGA CCTGGGACCC CTTACTTGCA GTGCCCGAAA CCGCCACGGC 
ACAAAGGCCT GCTCGGTCAC CCTGGAGCTG GCAGAGGCAC CTCGGTTTGA GTCTATCATG 
GAAGATGTGG AGGTGGGGCC TGGAGAAACC GCTCGCTTTG CTGTGGTAGT TGAGGGGAAA 
CCACTGCCAG ACATCATGTG GTACAAGGAT GAGGTGCTGC TGGCCGAGAG CAACCATGTG 
AGCTTCGTGT ATGAGGAGAA CGAGTGTTCC CTAGTGCTGC TCAGCGCAGG AAGCCAGGAT 
GGAGGCGTCT ATACCTGTAC CGCCCGGAAC CTGGCAGGCG AAGTCTCCTG CAAGGCGGAG 
CTGTCTGTGC TTTCAGCTCA GACAGCCATG GAGGTCGAGG GGGTCGGAGA GGATGAGGAA 
CACCGAGGAA GAAGACTTAG CGACTACTAC GACATCCACC AAGAGATTGG CAGGGGTGCC 
TTCTCCTACC TGCGGCGTGT AGTGGAGAGG AGCTCTGGCC TGGAGTTTGC AGCCAAGTTC 
ATCCCTAGCC AGGCTAAGCC TAAAGCATCA GCAAGACGCG AGGCGCGCTT GCTGGCCCGG 
CTCCAGCATG GCTGTGTGCT CTACTTCCAC GAAGCCTTTG AGAGGCGCAG GGGACTTGTC 
ATAGTCACGG AGCTCTGCAC AGAAGAGCTT CTAGAGCGCA TGGCCAGGAA GCCCACCGTG 
TGTGAGTCTG AGACTCGGAC CTATATGCGT CAAGTGCTAG AGGGGATATG CTATCTGCAT 
CAGAGCCATG TGCTGCACCT GGATGTCAAG CCGGAGAACC TGCTTGTGTG GGATGGTGCA 
GGGGGTGAAG AGCAGGTGCG CATCTGTGAC TTCGGGAACG CCCAGGAACT CACTCCGGGA 
GAGCCCCAGT ACTGTCAGTA TGGCACGCCA GAGTTTGTAG CACCTGAGAT TGTCAACCAG 
AGCCCTGTGT CTGGAGTCAC TGACATCTGG CCCGTGGGCG TCGTTGCCTT TCTCTGTTTG 
ACGGGCATCT CCCCATTTGT TGGGGAGAAT GACCGGACAA CACTCATGAA TATCCGAAAC 
TACAACGTGG CATTTGAGGA GACTACGTTC CTGAGTCTGA GCAGGGAGGC CCGGGGCTTC 
CTCATCAAAG TGCTGGTGCA GGATCGACTG AGACCTACTG CAGAAGAGAC ATTGGAACAT 
CCTTGGTTCA AAACAGAAGC AAAGGGTGCA GAGGTGAGCA CAGATCACCT GAAGCTCTTC 
CTGTCACGGA GGAGGTGGCA GCGCTCTCAA ATCAGCTACA AGTGCCACCT GGTGCTGCGC 
CCCATTCCGG AACTGCTACG GGCTCCCCCG GAGCGGGTGT GGGTGGCCAT GCCTAGAAGA 
CAACCCCCGA GTGGGGGACT CTCATCCTCT TCAGACTCAG AAGAGGAAGA GTTGGAGGAA 
CTTCCCTCAG TACCCCGCCC CCTGCAGCCT GAGTTCTCAG GTTCTCGGGT ATCCCTCACC 
GACATCCCCA CCGAAGATGA GGCTCTGGGG ACCCCGGAAG CTGGGGCTGC CACCCCCATG 
GACTGGCAAG AGCAGGAAAG GACTCCCTCT AAGGACCAGG AGGCCCCCAG CCCTGAGGCA 
CTCCCCTCTC CAGGCCAGGA GTCCCCTGAT GGGCCTAGCC CCAGGCGGCC AGAGCTCCGC 
AGGGGTAGCT CTGCTGAGAG TGCCCTGCCC CGAGTCGGGT CGCGGGAACC GGGCCGGAGC 
TTGCACAAGG CAGCATCCGT GGAGCTGCCG CAGCGCAGGA GCCCCAGCCC GGGTGCCACC 
CGCCTGACTC GGGGAGGCCT GGGTGAGGGC GAGTATGCCC AGAGGCTGCA GGCCCTGCGT 
CAGCGGCTGC TGAGGGGAGG CCCCGAGGAT GGCAAGGTCA GCGGCCTCAG GGGACCCCTG 
CTGGAGAGCC TGGGGGGCCG TGCACGGGAT CCCAGGATGG CAAGGGCTGC CTCCAGCGAG 
GCTGCCCCCC ACCACCAGCC CCCTCCGGAG TCCCGGGGTC TGCAAAAGAG CAGCAGCTTC 
TCTCAGGGCG AGGCAGAGCC CCGGGGTCGG CACCGCCGCG CGGGGGCACC CCTCGAAATC 
CCCGTGGCCA GACTTGGGGC TCGCAGGCTG CAGGAGTCTC CCTCCTTATC TGCCCTTAGC 
GAGACCCAGC CACCGAGCCC TGCGAGGCCC AGCGTCCCCA AACTCAGTAT CACCAAGTCC 
CCGGAACCTT CCGCTGTCAC ATCCAGAGAC TCTCCCCAGC CCCCGGAACC TCAGCCTGTC 
CCAGAAAAGG TCCCTGAGCC CAAACCAGAA CCAGTCCGAG CTGCAAAACC AGCACAACCC 
CCTTTGGCTC TGCAAATGCC AACGCAGCCT CTCACCCCCT ATGCCCAGAT CATGCAGTCG 
CTCCAGCTGT CCAGTCCCAC CCTGTCCCCA CAGGACCCCG CTGTGCCTCC ATCAGAGCCC 
AAGCCCCACG CGGCTGTGTT TGCCAGGGTG GCTTCCCCAC CACCCGGAGT CTCTGAGAAG 
CGCGTGCCTT CGGCCAGAAC TCCCCCAGTG CTAGCCGAGA AAGCCCGAGT CCCCACGGTG 
CCCCCCAGGC CAGGCAGCAG CCTCAGCGGC AGCATCGAAA ACCTTGAATC AGAGGCTGTG 
TTCGAGGCTA AGTTCAAACG CAGCCGCGAA TCGCCACTGT CGCGGGGCCT GAGGCTACTG 
AGCCGCTCGC GCTCGGAGGA GCGCGGTCCC TTCCGTGGGG CGGAGGACGA CGGCATATAC 
CGGCCCAGTC CGGCAGGAAC GCCGCTAGAG CTGGTGCGAC GGCCCGAGCG CTCGCGCTCC 
GTGCAGGACC TCAGGGTAGC CGGAGAGCCG GGCCTTGTGC GTCGCCTTTC GCTGTCCCTG 
TCGCAGAAGC TGCGGCGGAC ACCGCCGGGA CAGCGACACC CTGCCTGGGA GTCCCGCAGC 
GGAGACGGGG AGAGCTCCGA GGGCGGCAGC TCAGCTCGGG CATCCCCAGT GCTGGCGGTA 
CGCAGGAGAC TCAGCTCCAC GCTGGAGCGT CTGTCCAGCC GTTTGCAGCG CAGCGGCAGC 
AGCGAGGACT CCGGGGGCGC CTCGGGTCGC AGCACGCCGC TGTTTGGACG GCTGCGCAGG 
GCTACTTCGG AAGGGGAGAG CCTGCGACGC CTCGGTGTTC CGCACAATCA GCTGGGCTCT 
CAGACTGGTG CCACTACACC TTCCGCTGAG TCTCTGGGCT CAGAGGCCAG TGGCACATCG 
GGTTCTTCAG CTCCTGGAGA AAGCCGAAGC CGGCACCGGT GGGGCCTTTC CAGGCTGCGC 
AAGGACAAGG GATTATCTCA GCCAAACCTC TCCTCCAGTG TCCAAGAGGA CTTGGGTCAC 
CAGTATGTGC CCAGTGAGTC AGATTTCCCC CCAGTCTTCC ACATCAAACT CAAGGACCAG 
GTGCTGTTGG AGGGGGAAGC AGCCACCCTG CTCTGCCTAC CCGCAGCCTG CCCAGCACCC 
CGCATCTCCT GGATGAAAGA TAAGCAGTCC CTGCGTTCAG AGCCCTCAGT GGTCATTGTG 
TCCTGCAAAG ATGGACGGCA GCTGCTGAGC ATACCCCGGG CCGGCAAGCG GCATGCTGGG 
CTCTATGAGT GCTCAGCCAC CAATGTCCTG GGCAGCATTA CCAGCTCCTG TACTGTGGCC 
GTGGCCCGCA TTCCAGGGAA GCTAGCTCCT CCAGAGGTGC CCCAGACCTA CCATGACACA 
GCACTAGTAG TGTGGAAGCC AGGAGACGGT CGGGCACCTT GCACATACAC TCTGGAGCGG 
CGGGTGGATG GGGAATCTGT CTGGCACCCT GTGAGCTCAG GCATCCCCGA CTGTTATTAC 
AATGTGACTC AGCTGCCTGT CGGCGTGACC GTGCGGTTCC GTGTGGCCTG TTCCAATCGT 
GCTGGGCAGG GGCCCTTCAG CAACCCTTCT GAGAAGGTCT TCATCCGAGG CACTCCAGAT 
TCTCCTGCTC AGCCAGCTGC TGCTCCCCGA GATGCCCCTG TTACCTCAGG GCCAACCAGG 
GCCCCACCGC CTGACTCTCC TACCTCACTG GCCCCCACCC CCGCTCTTGC GCCCCCAGCC 
TCCCAGGCAT CCACTCTCAG CCCCTCAACT TCCTCCATGT CTGCCAACCA GGCCTTGTCC 
TCACTCAAGG CTGTGGGTCC ACCACCCGCA ACCCCTCCAC GAAAGCACAG GGGCCTTCTG 
GCCACCCAGC AAGCAGAGCC ATCCCCACCC AGTATCGTGG TCACCCCAAG TGAACCCAGG 
TCTTTTGTCC CTGACACTGG AACCCTGACC CCAACCTCTA GTCCTCAAGG GGTTAAACCA 
GCACCTTCCT CAACTTCCTT GTATATGGTG ACTTCCTTCG TGTCTGCACC TCCAGCCCCC 
CAGGCCCCAG CCCCTGAGCC CCCTCCTGAG CCCACCAAGG TGACTGTGCG GAGCCTCAGC 
CCAGCCAAGG AGGTGGTCAG CTCCCCAACT CCTGAGAGCA CCACTCTTCG GCAGGGTCCC 
CCTCAGAAAC CCTACACCTT CCTGGAGGAG AAGGCCAGGG GCCGCTTTGG CGTTGTGCGG 
TCATGCCGGG AGAATGCTAC GGGCCGAACG TTTGTCGCCA AGATCGTGCC GTACGCCGCG 
GAGGGCAAGC GACGCGTGCT GCAGGAATAC GAGGTGCTTC GGACGCTGCA CCACGAGCGG 
CTCATGTCCC TGCACGAGGC CTACATCACC CCTCGGTACC TCGTGCTTAT CGCTGAGAGC 
TGTGGCAACC GGGAACTCCT CTGTGGGCTC AGCGACAGAT TCCGGTATTC AGAAGATGAC 
GTGGCCACCT ATGTGGTGCA GCTGCTACAA GGCCTGGACT ACCTCCACGG CCACCACGTG 
CTACACCTGG ACATCAAGCC GGACAATCTA CTGTTGGCCG CTGACAATGC CCTTAAAATT 
GTGGACTTCG GCAGTGCTCA GCCCTATAAC CCTCAGGCCC TGAAGCCACT TGGCCACCGC 
ACAGGCACTC TGGAGTTCAT GGCTCCTGAG ATGGTGAAGG GAGACCCCAT TGGCTCTGCC 
ACGGACATCT GGGGAGCGGG TGTGCTCACC TACATCATGC TTAGTGGGTA CTCCCCATTC 
TATGAGCCAG ACCCCCAGGA AACAGAGGCT CGGATTGTTG GGGGTCGCTT TGATGCCTTC 
CAGTTGTATC CTAACACATC CCAGAGTGCC ACCCTCTTCT TGAGAAAGGT CCTCTCAGTA 
CATCCCTGGA GCCGGCCCTC TCTGCAGGAC TGCTTGGCCC ACCCATGGCT GCAAGATGCC 
TACCTGATGA AGCTGCGCCG CCAGACACTC ACCTTCACCA CCAACCGGCT CAAGGAATTC 
CTGGGCGAGC AGCGGCGACG TCGGGCTGAG GCTGCTACCC GTCACAAGGT GCTGCTCCGC 
TCCTACCCTG GCAGCCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_011238645.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236947.1 Mus musculus SPEG complex locus (Speg), transcript variant X5, mRNA.

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CloneID	OMu67146
Clone ID Related Accession (Same CDS sequence)	XM_011238645.1 , XM_011238645.2
Accession Version	XM_011238645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA 
GCACAGACAA CCGGGGCCGG GCCACGGCAC CTGGGGGTGG AGCCGCTGGT GCGGGCATCG 
CGAGCTAATC TGGTGGGCGC AAGCTGGGGG TCAGAGGATA GCCTTTCGGT GGCCAGTGAC 
CTCTATGGCA GCGCGTTCAG TCTGTACAGA GGACGGGCGC TCTCGATCCA CGTCAGCATT 
CCTCCGAGTG GATTGCACAG AGAGGAGCCT GACCTTCAGC CTCAGCCAGC CAGTGATGCC 
CTTCGTCCAC GCCCTGCCCT TCCACCTCCC TCCAAATCCG CTTTGCTTCC CCCACCATCT 
CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA CCTAGTACCC AGCCCCCAGC TACTCCCACT 
TCGCCCCATC GGCGTGCTCA GGAGCCCTCG CTGCCTGAGG ACATCACCAC CACGGAAGAG 
AAGCGAGGGA AAAAGCCCAA GTCATCCGGG CCGTCGCTGG CGGGCACAGT GGAGTCCCGG 
CCCCAGACGC CACTGAGCGA GGCTTCGGGT CGCCTGTCAG CATTGGGCCG CTCGCCCCGG 
CTGGTGCGCG CGGGGTCCCG CATCCTGGAC AAGCTACAGT TCTTCGAAGA GCGGCGACGC 
AGCCTGGAGC GCAGCGACTC GCCGCCAGCG CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG 
GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC 
TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG 
CAAAAGGCGG CCTCGTTGGA TGAACGCACG CGACAACGCA GTGCAACCTC GGACCTCGAA 
CTCCGCTTCG CCCAGGAGCT GGGTCGCATC CGCCGATCTA CGTCGCGGGA GGAGCTGGTG 
CGTTCGCACG AGTCCCTGCG TGCCACGCTG CAGCGCGCCC CATCCCCTCG GGAGCCCGGC 
GAGCCCCCAC TCTTCTCCCG GCCTTCCACA CCCAAGACCT CACGGGCTGT GAGCCCGGCT 
GCCACCCAGC CGCCGCCTCC TAGTGGTGCG GGCAAATCTG GGGACGAGCC TGGGAGGCCC 
CGAAGCAGAG GGCCGGTGGG CAGGACTGAA CCGGGGGAAG GCCCGCAGCA GGAGATCAAG 
CGTCGGGACC AATTCCCGCT AACCAGGAGC AGAGCCATCC AGGAGTGCAG GAGCCCTGTG 
CCGCCCTACA CCGCGGATCC CCCGGAGAGC AGGACAAAAG CCCCCTCCGG TCGCAAGCGG 
GAACCCCCTG CTCAAGCGGT GCGCTTTCTG CCCTGGGCCA CTCCGGGAGT GGAGGACTCT 
GTTCTGCCCC AAACCTTGGA GAAGAATAGA GCGGGACCCG AGGCTGAGAA GAGGCTTCGC 
AGAGGACCTG AGGAGGATGG CCCCTGGGGG CCCTGGGACC GCAGAGGGAC CCGCAGCCAA 
GGCAAAGGTC GCCGTGCTCG GCCTACTTCC CCCGAGCTCG AGTCCTCAGA CGACTCCTAT 
GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA CCCGTGTTTG AGATCCCTCT GCAGAATATG 
GTGGTGGCGC CAGGAGCTGA CGTGCTACTT AAGTGTATCA TCACCGCCAA CCCCCCACCC 
CAAGTGTCCT GGAAAAAGGA TGGGTCCATG TTGCACAGCG AGGGTCGTCT TCTCATCCGG 
GCTGAAGGTG AACGGCACAC ACTGCTGCTC AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC 
TACACAGCCA CTGCCACCAA CGAACTGGGC CAAGCTACCT GTGCTTCTTC ACTGGCTGTG 
AGACCTGGCG GCTCCACATC CCCTTTCAGC AGCCCCATCA CCTCTGATGA GGAGTACCTG 
AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG GAGACCTGGC CCCGAACCCC TACCATGAAG 
CTCAGTCCCA GCCAGGATCA TGATTCCTCC GACTCTTCTT CCAAGGCACC CCCAACGTTC 
AAGGTCTCAC TCATGGACCA ATCGGTGAGA GAAGGTCAAG ATGTCATTAT GAGCATCCGT 
GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC TGGCTGAGGA ATCGACAGCC CGTGCGCCCA 
GACCAGCGGC GCTTTGCAGA GGAGGCCGAG GGTGGGCTCT GCCGCTTGAG GATCCTGGCT 
GCTGAACGGG GCGATGCTGG TTTCTACACA TGCAAGGCGG TCAACGAATA TGGCGCTCGG 
CAGTGCGAGG CCCGCCTGGA GGTCCGAGCA CACCCTGAAA GCCGGTCCCT GGCCGTGCTG 
GCCCCCCTGC AGGACGTGGA CGTGGGGGCC GGGGAGATGG CGCTGTTTGA GTGCCTGGTG 
GCAGGTCCAG CTGACGTCGA GGTGGACTGG CTGTGCCGGG GCCGCCTGCT GCAGCCTGCG 
CTGCTCAAAT GCAAGATGCA TTTTGATGGC CGCAAGTGCA AGCTGCTGCT CACCTCTGTG 
CACGAGGACG ACAGTGGTGT CTACACCTGC AAGCTGAGCA CAGCCAAAGA TGAACTGACC 
TGCAGTGCCC GGCTGACGGT TCGGCCGTCG CTGGCGCCTC TCTTCACCCG GTTGCTGGAA 
GATGTGGAGG TGTTGGAAGG CCGTGCTGCC CGCTTGGATT GCAAGATCAG CGGTACCCCG 
CCTCCCTCCG TTACCTGGAC TCATTTTGGC CACCCCGTGA ATGAGGGTGA CAACTTGCGG 
CTTCAACAGG ATGGAGGTCT ACACTCACTG CACATTGCCC GGGTGGGCAG TGAGGACGAA 
GGACTCTATG AAGTCAGTGC CACCAACACT CATGGCCAGG CCCACTGCTC CGCCCAGCTG 
TATGTGGAGG AGCCTCGGAC AGCTGCCTCT GGCCCCAGCT CGAAGCTGGA GAAGATGCCA 
TCTATCCCTG AGGAGCCGGA GCACGGAGAT CTAGAGAGGC TGTCTATTCC TGACTTCCTA 
CGGCCACTGC AAGATCTGGA AGTGGGACTA GCTAAGGAGG CCATGCTGGA GTGCCAAGTG 
ACCGGCCTGC CCTATCCCAC CATCAGCTGG TTCCACAATG GCCACCGCAT TCAAAGCAGT 
GACGACCGAC GCATGACACA GTACAGGGAC ATACATCGCC TGGTGTTCCC AGCTGTGGGG 
CCTCAGCATG CTGGTGTCTA TAAGAGTGTA ATCGCCAACA AGCTGGGCAA AGCGGCCTGC 
TACGCCCATC TCTATGTCAC AGATGTGGTT CCAGGCCCTC CAGATGGTGC TCCGGAAGTA 
GTAGCTGTGA CCGGAAGGAT GGTCACACTA TCGTGGAACC CACCCAGGAG TCTGGACATG 
GCTATTGACC CAGATTCCCT AACATACACT GTGCAGCACC AGGTGTTGGG CTCGGACCAG 
TGGACGGCAC TGGTCACAGG CCTGCGGGAG CCTGCGTGGG CAGCCACAGG GCTGAAGAAG 
GGGATCCAGC ACATCTTCCG GGTCCTCAGC TCCAGTGGCA AGAGCAGCAG CAAGCCCTCA 
GCACCGTCGG AACCCGTGCA GCTGCTAGAG CATGGCCCAC CCCTGGAAGA GGCGCCTGCT 
GTGCTGGACA AGCAGGACAT TGTGTATGTG GTAGAGGGCC AGCCTGCCTG TGTCACCGTC 
ACTTTTAACC ATGTGGAGGC CCAGGTTGTC TGGAGGAGCT GCCGGGGAGC CCTCCTCGAG 
GCGCGCACAG GTGTGTATGA ACTGAGTCAG CCAGATGACG ACCAGTATTG TCTGCGGATC 
TGCCGGGTGA GCCGACGAGA CCTGGGACCC CTTACTTGCA GTGCCCGAAA CCGCCACGGC 
ACAAAGGCCT GCTCGGTCAC CCTGGAGCTG GCAGAGGCAC CTCGGTTTGA GTCTATCATG 
GAAGATGTGG AGGTGGGGCC TGGAGAAACC GCTCGCTTTG CTGTGGTAGT TGAGGGGAAA 
CCACTGCCAG ACATCATGTG GTACAAGGAT GAGGTGCTGC TGGCCGAGAG CAACCATGTG 
AGCTTCGTGT ATGAGGAGAA CGAGTGTTCC CTAGTGCTGC TCAGCGCAGG AAGCCAGGAT 
GGAGGCGTCT ATACCTGTAC CGCCCGGAAC CTGGCAGGCG AAGTCTCCTG CAAGGCGGAG 
CTGTCTGTGC TTTCAGCTCA GACAGCCATG GAGGTCGAGG GGGTCGGAGA GGATGAGGAA 
CACCGAGGAA GAAGACTTAG CGACTACTAC GACATCCACC AAGAGATTGG CAGGGGTGCC 
TTCTCCTACC TGCGGCGTGT AGTGGAGAGG AGCTCTGGCC TGGAGTTTGC AGCCAAGTTC 
ATCCCTAGCC AGGCTAAGCC TAAAGCATCA GCAAGACGCG AGGCGCGCTT GCTGGCCCGG 
CTCCAGCATG GCTGTGTGCT CTACTTCCAC GAAGCCTTTG AGAGGCGCAG GGGACTTGTC 
ATAGTCACGG AGCTCTGCAC AGAAGAGCTT CTAGAGCGCA TGGCCAGGAA GCCCACCGTG 
TGTGAGTCTG AGACTCGGAC CTATATGCGT CAAGTGCTAG AGGGGATATG CTATCTGCAT 
CAGAGCCATG TGCTGCACCT GGATGTCAAG CCGGAGAACC TGCTTGTGTG GGATGGTGCA 
GGGGGTGAAG AGCAGGTGCG CATCTGTGAC TTCGGGAACG CCCAGGAACT CACTCCGGGA 
GAGCCCCAGT ACTGTCAGTA TGGCACGCCA GAGTTTGTAG CACCTGAGAT TGTCAACCAG 
AGCCCTGTGT CTGGAGTCAC TGACATCTGG CCCGTGGGCG TCGTTGCCTT TCTCTGTTTG 
ACGGGCATCT CCCCATTTGT TGGGGAGAAT GACCGGACAA CACTCATGAA TATCCGAAAC 
TACAACGTGG CATTTGAGGA GACTACGTTC CTGAGTCTGA GCAGGGAGGC CCGGGGCTTC 
CTCATCAAAG TGCTGGTGCA GGATCGACTG AGACCTACTG CAGAAGAGAC ATTGGAACAT 
CCTTGGTTCA AAACAGAAGC AAAGGGTGCA GAGGTGAGCA CAGATCACCT GAAGCTCTTC 
CTGTCACGGA GGAGGTGGCA GCGCTCTCAA ATCAGCTACA AGTGCCACCT GGTGCTGCGC 
CCCATTCCGG AACTGCTACG GGCTCCCCCG GAGCGGGTGT GGGTGGCCAT GCCTAGAAGA 
CAACCCCCGA GTGGGGGACT CTCATCCTCT TCAGACTCAG AAGAGGAAGA GTTGGAGGAA 
CTTCCCTCAG TACCCCGCCC CCTGCAGCCT GAGTTCTCAG GTTCTCGGGT ATCCCTCACC 
GACATCCCCA CCGAAGATGA GGCTCTGGGG ACCCCGGAAG CTGGGGCTGC CACCCCCATG 
GACTGGCAAG AGCAGGAAAG GACTCCCTCT AAGGACCAGG AGGCCCCCAG CCCTGAGGCA 
CTCCCCTCTC CAGGCCAGGA GTCCCCTGAT GGGCCTAGCC CCAGGCGGCC AGAGCTCCGC 
AGGGGTAGCT CTGCTGAGAG TGCCCTGCCC CGAGTCGGGT CGCGGGAACC GGGCCGGAGC 
TTGCACAAGG CAGCATCCGT GGAGCTGCCG CAGCGCAGGA GCCCCAGCCC GGGTGCCACC 
CGCCTGACTC GGGGAGGCCT GGGTGAGGGC GAGTATGCCC AGAGGCTGCA GGCCCTGCGT 
CAGCGGCTGC TGAGGGGAGG CCCCGAGGAT GGCAAGGTCA GCGGCCTCAG GGGACCCCTG 
CTGGAGAGCC TGGGGGGCCG TGCACGGGAT CCCAGGATGG CAAGGGCTGC CTCCAGCGAG 
GCTGCCCCCC ACCACCAGCC CCCTCCGGAG TCCCGGGGTC TGCAAAAGAG CAGCAGCTTC 
TCTCAGGGCG AGGCAGAGCC CCGGGGTCGG CACCGCCGCG CGGGGGCACC CCTCGAAATC 
CCCGTGGCCA GACTTGGGGC TCGCAGGCTG CAGGAGTCTC CCTCCTTATC TGCCCTTAGC 
GAGACCCAGC CACCGAGCCC TGCGAGGCCC AGCGTCCCCA AACTCAGTAT CACCAAGTCC 
CCGGAACCTT CCGCTGTCAC ATCCAGAGAC TCTCCCCAGC CCCCGGAACC TCAGCCTGTC 
CCAGAAAAGG TCCCTGAGCC CAAACCAGAA CCAGTCCGAG CTGCAAAACC AGCACAACCC 
CCTTTGGCTC TGCAAATGCC AACGCAGCCT CTCACCCCCT ATGCCCAGAT CATGCAGTCG 
CTCCAGCTGT CCAGTCCCAC CCTGTCCCCA CAGGACCCCG CTGTGCCTCC ATCAGAGCCC 
AAGCCCCACG CGGCTGTGTT TGCCAGGGTG GCTTCCCCAC CACCCGGAGT CTCTGAGAAG 
CGCGTGCCTT CGGCCAGAAC TCCCCCAGTG CTAGCCGAGA AAGCCCGAGT CCCCACGGTG 
CCCCCCAGGC CAGGCAGCAG CCTCAGCGGC AGCATCGAAA ACCTTGAATC AGAGGCTGTG 
TTCGAGGCTA AGTTCAAACG CAGCCGCGAA TCGCCACTGT CGCGGGGCCT GAGGCTACTG 
AGCCGCTCGC GCTCGGAGGA GCGCGGTCCC TTCCGTGGGG CGGAGGACGA CGGCATATAC 
CGGCCCAGTC CGGCAGGAAC GCCGCTAGAG CTGGTGCGAC GGCCCGAGCG CTCGCGCTCC 
GTGCAGGACC TCAGGGTAGC CGGAGAGCCG GGCCTTGTGC GTCGCCTTTC GCTGTCCCTG 
TCGCAGAAGC TGCGGCGGAC ACCGCCGGGA CAGCGACACC CTGCCTGGGA GTCCCGCAGC 
GGAGACGGGG AGAGCTCCGA GGGCGGCAGC TCAGCTCGGG CATCCCCAGT GCTGGCGGTA 
CGCAGGAGAC TCAGCTCCAC GCTGGAGCGT CTGTCCAGCC GTTTGCAGCG CAGCGGCAGC 
AGCGAGGACT CCGGGGGCGC CTCGGGTCGC AGCACGCCGC TGTTTGGACG GCTGCGCAGG 
GCTACTTCGG AAGGGGAGAG CCTGCGACGC CTCGGTGTTC CGCACAATCA GCTGGGCTCT 
CAGACTGGTG CCACTACACC TTCCGCTGAG TCTCTGGGCT CAGAGGCCAG TGGCACATCG 
GGTTCTTCAG CTCCTGGAGA AAGCCGAAGC CGGCACCGGT GGGGCCTTTC CAGGCTGCGC 
AAGGACAAGG GATTATCTCA GCCAAACCTC TCCTCCAGTG TCCAAGAGGA CTTGGGTCAC 
CAGTATGTGC CCAGTGAGTC AGATTTCCCC CCAGTCTTCC ACATCAAACT CAAGGACCAG 
GTGCTGTTGG AGGGGGAAGC AGCCACCCTG CTCTGCCTAC CCGCAGCCTG CCCAGCACCC 
CGCATCTCCT GGATGAAAGA TAAGCAGTCC CTGCGTTCAG AGCCCTCAGT GGTCATTGTG 
TCCTGCAAAG ATGGACGGCA GCTGCTGAGC ATACCCCGGG CCGGCAAGCG GCATGCTGGG 
CTCTATGAGT GCTCAGCCAC CAATGTCCTG GGCAGCATTA CCAGCTCCTG TACTGTGGCC 
GTGGCCCGCA TTCCAGGGAA GCTAGCTCCT CCAGAGGTGC CCCAGACCTA CCATGACACA 
GCACTAGTAG TGTGGAAGCC AGGAGACGGT CGGGCACCTT GCACATACAC TCTGGAGCGG 
CGGGTGGATG GGGAATCTGT CTGGCACCCT GTGAGCTCAG GCATCCCCGA CTGTTATTAC 
AATGTGACTC AGCTGCCTGT CGGCGTGACC GTGCGGTTCC GTGTGGCCTG TTCCAATCGT 
GCTGGGCAGG GGCCCTTCAG CAACCCTTCT GAGAAGGTCT TCATCCGAGG CACTCCAGAT 
TCTCCTGCTC AGCCAGCTGC TGCTCCCCGA GATGCCCCTG TTACCTCAGG GCCAACCAGG 
GCCCCACCGC CTGACTCTCC TACCTCACTG GCCCCCACCC CCGCTCTTGC GCCCCCAGCC 
TCCCAGGCAT CCACTCTCAG CCCCTCAACT TCCTCCATGT CTGCCAACCA GGCCTTGTCC 
TCACTCAAGG CTGTGGGTCC ACCACCCGCA ACCCCTCCAC GAAAGCACAG GGGCCTTCTG 
GCCACCCAGC AAGCAGAGCC ATCCCCACCC AGTATCGTGG TCACCCCAAG TGAACCCAGG 
TCTTTTGTCC CTGACACTGG AACCCTGACC CCAACCTCTA GTCCTCAAGG GGTTAAACCA 
GCACCTTCCT CAACTTCCTT GTATATGGTG ACTTCCTTCG TGTCTGCACC TCCAGCCCCC 
CAGGCCCCAG CCCCTGAGCC CCCTCCTGAG CCCACCAAGG TGACTGTGCG GAGCCTCAGC 
CCAGCCAAGG AGGTGGTCAG CTCCCCAACT CCTGAGAGCA CCACTCTTCG GCAGGGTCCC 
CCTCAGAAAC CCTACACCTT CCTGGAGGAG AAGGCCAGGG GCCGCTTTGG CGTTGTGCGG 
TCATGCCGGG AGAATGCTAC GGGCCGAACG TTTGTCGCCA AGATCGTGCC GTACGCCGCG 
GAGGGCAAGC GACGCGTGCT GCAGGAATAC GAGGTGCTTC GGACGCTGCA CCACGAGCGG 
CTCATGTCCC TGCACGAGGC CTACATCACC CCTCGGTACC TCGTGCTTAT CGCTGAGAGC 
TGTGGCAACC GGGAACTCCT CTGTGGGCTC AGCGACAGAT TCCGGTATTC AGAAGATGAC 
GTGGCCACCT ATGTGGTGCA GCTGCTACAA GGCCTGGACT ACCTCCACGG CCACCACGTG 
CTACACCTGG ACATCAAGCC GGACAATCTA CTGTTGGCCG CTGACAATGC CCTTAAAATT 
GTGGACTTCG GCAGTGCTCA GCCCTATAAC CCTCAGGCCC TGAAGCCACT TGGCCACCGC 
ACAGGCACTC TGGAGTTCAT GGCTCCTGAG ATGGTGAAGG GAGACCCCAT TGGCTCTGCC 
ACGGACATCT GGGGAGCGGG TGTGCTCACC TACATCATGC TTAGTGGGTA CTCCCCATTC 
TATGAGCCAG ACCCCCAGGA AACAGAGGCT CGGATTGTTG GGGGTCGCTT TGATGCCTTC 
CAGTTGTATC CTAACACATC CCAGAGTGCC ACCCTCTTCT TGAGAAAGGT CCTCTCAGTA 
CATCCCTGGA GCCGGCCCTC TCTGCAGGAC TGCTTGGCCC ACCCATGGCT GCAAGATGCC 
TACCTGATGA AGCTGCGCCG CCAGACACTC ACCTTCACCA CCAACCGGCT CAAGGAATTC 
CTGGGCGAGC AGCGGCGACG TCGGGCTGAG GCTGCTACCC GTCACAAGGT GCTGCTCCGC 
TCCTACCCTG GCAGCCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236947.1
CDS	1364..10864
Translation	MKKLWVKKRFQKTGHSRRAFGRLTHVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPRQAQTTGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_011238645.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238645.2

ORF Insert Sequence:

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA 
GCACAGACAA CCGGGGCCGG GCCACGGCAC CTGGGGGTGG AGCCGCTGGT GCGGGCATCG 
CGAGCTAATC TGGTGGGCGC AAGCTGGGGG TCAGAGGATA GCCTTTCGGT GGCCAGTGAC 
CTCTATGGCA GCGCGTTCAG TCTGTACAGA GGACGGGCGC TCTCGATCCA CGTCAGCATT 
CCTCCGAGTG GATTGCACAG AGAGGAGCCT GACCTTCAGC CTCAGCCAGC CAGTGATGCC 
CTTCGTCCAC GCCCTGCCCT TCCACCTCCC TCCAAATCCG CTTTGCTTCC CCCACCATCT 
CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA CCTAGTACCC AGCCCCCAGC TACTCCCACT 
TCGCCCCATC GGCGTGCTCA GGAGCCCTCG CTGCCTGAGG ACATCACCAC CACGGAAGAG 
AAGCGAGGGA AAAAGCCCAA GTCATCCGGG CCGTCGCTGG CGGGCACAGT GGAGTCCCGG 
CCCCAGACGC CACTGAGCGA GGCTTCGGGT CGCCTGTCAG CATTGGGCCG CTCGCCCCGG 
CTGGTGCGCG CGGGGTCCCG CATCCTGGAC AAGCTACAGT TCTTCGAAGA GCGGCGACGC 
AGCCTGGAGC GCAGCGACTC GCCGCCAGCG CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG 
GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC 
TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG 
CAAAAGGCGG CCTCGTTGGA TGAACGCACG CGACAACGCA GTGCAACCTC GGACCTCGAA 
CTCCGCTTCG CCCAGGAGCT GGGTCGCATC CGCCGATCTA CGTCGCGGGA GGAGCTGGTG 
CGTTCGCACG AGTCCCTGCG TGCCACGCTG CAGCGCGCCC CATCCCCTCG GGAGCCCGGC 
GAGCCCCCAC TCTTCTCCCG GCCTTCCACA CCCAAGACCT CACGGGCTGT GAGCCCGGCT 
GCCACCCAGC CGCCGCCTCC TAGTGGTGCG GGCAAATCTG GGGACGAGCC TGGGAGGCCC 
CGAAGCAGAG GGCCGGTGGG CAGGACTGAA CCGGGGGAAG GCCCGCAGCA GGAGATCAAG 
CGTCGGGACC AATTCCCGCT AACCAGGAGC AGAGCCATCC AGGAGTGCAG GAGCCCTGTG 
CCGCCCTACA CCGCGGATCC CCCGGAGAGC AGGACAAAAG CCCCCTCCGG TCGCAAGCGG 
GAACCCCCTG CTCAAGCGGT GCGCTTTCTG CCCTGGGCCA CTCCGGGAGT GGAGGACTCT 
GTTCTGCCCC AAACCTTGGA GAAGAATAGA GCGGGACCCG AGGCTGAGAA GAGGCTTCGC 
AGAGGACCTG AGGAGGATGG CCCCTGGGGG CCCTGGGACC GCAGAGGGAC CCGCAGCCAA 
GGCAAAGGTC GCCGTGCTCG GCCTACTTCC CCCGAGCTCG AGTCCTCAGA CGACTCCTAT 
GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA CCCGTGTTTG AGATCCCTCT GCAGAATATG 
GTGGTGGCGC CAGGAGCTGA CGTGCTACTT AAGTGTATCA TCACCGCCAA CCCCCCACCC 
CAAGTGTCCT GGAAAAAGGA TGGGTCCATG TTGCACAGCG AGGGTCGTCT TCTCATCCGG 
GCTGAAGGTG AACGGCACAC ACTGCTGCTC AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC 
TACACAGCCA CTGCCACCAA CGAACTGGGC CAAGCTACCT GTGCTTCTTC ACTGGCTGTG 
AGACCTGGCG GCTCCACATC CCCTTTCAGC AGCCCCATCA CCTCTGATGA GGAGTACCTG 
AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG GAGACCTGGC CCCGAACCCC TACCATGAAG 
CTCAGTCCCA GCCAGGATCA TGATTCCTCC GACTCTTCTT CCAAGGCACC CCCAACGTTC 
AAGGTCTCAC TCATGGACCA ATCGGTGAGA GAAGGTCAAG ATGTCATTAT GAGCATCCGT 
GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC TGGCTGAGGA ATCGACAGCC CGTGCGCCCA 
GACCAGCGGC GCTTTGCAGA GGAGGCCGAG GGTGGGCTCT GCCGCTTGAG GATCCTGGCT 
GCTGAACGGG GCGATGCTGG TTTCTACACA TGCAAGGCGG TCAACGAATA TGGCGCTCGG 
CAGTGCGAGG CCCGCCTGGA GGTCCGAGCA CACCCTGAAA GCCGGTCCCT GGCCGTGCTG 
GCCCCCCTGC AGGACGTGGA CGTGGGGGCC GGGGAGATGG CGCTGTTTGA GTGCCTGGTG 
GCAGGTCCAG CTGACGTCGA GGTGGACTGG CTGTGCCGGG GCCGCCTGCT GCAGCCTGCG 
CTGCTCAAAT GCAAGATGCA TTTTGATGGC CGCAAGTGCA AGCTGCTGCT CACCTCTGTG 
CACGAGGACG ACAGTGGTGT CTACACCTGC AAGCTGAGCA CAGCCAAAGA TGAACTGACC 
TGCAGTGCCC GGCTGACGGT TCGGCCGTCG CTGGCGCCTC TCTTCACCCG GTTGCTGGAA 
GATGTGGAGG TGTTGGAAGG CCGTGCTGCC CGCTTGGATT GCAAGATCAG CGGTACCCCG 
CCTCCCTCCG TTACCTGGAC TCATTTTGGC CACCCCGTGA ATGAGGGTGA CAACTTGCGG 
CTTCAACAGG ATGGAGGTCT ACACTCACTG CACATTGCCC GGGTGGGCAG TGAGGACGAA 
GGACTCTATG AAGTCAGTGC CACCAACACT CATGGCCAGG CCCACTGCTC CGCCCAGCTG 
TATGTGGAGG AGCCTCGGAC AGCTGCCTCT GGCCCCAGCT CGAAGCTGGA GAAGATGCCA 
TCTATCCCTG AGGAGCCGGA GCACGGAGAT CTAGAGAGGC TGTCTATTCC TGACTTCCTA 
CGGCCACTGC AAGATCTGGA AGTGGGACTA GCTAAGGAGG CCATGCTGGA GTGCCAAGTG 
ACCGGCCTGC CCTATCCCAC CATCAGCTGG TTCCACAATG GCCACCGCAT TCAAAGCAGT 
GACGACCGAC GCATGACACA GTACAGGGAC ATACATCGCC TGGTGTTCCC AGCTGTGGGG 
CCTCAGCATG CTGGTGTCTA TAAGAGTGTA ATCGCCAACA AGCTGGGCAA AGCGGCCTGC 
TACGCCCATC TCTATGTCAC AGATGTGGTT CCAGGCCCTC CAGATGGTGC TCCGGAAGTA 
GTAGCTGTGA CCGGAAGGAT GGTCACACTA TCGTGGAACC CACCCAGGAG TCTGGACATG 
GCTATTGACC CAGATTCCCT AACATACACT GTGCAGCACC AGGTGTTGGG CTCGGACCAG 
TGGACGGCAC TGGTCACAGG CCTGCGGGAG CCTGCGTGGG CAGCCACAGG GCTGAAGAAG 
GGGATCCAGC ACATCTTCCG GGTCCTCAGC TCCAGTGGCA AGAGCAGCAG CAAGCCCTCA 
GCACCGTCGG AACCCGTGCA GCTGCTAGAG CATGGCCCAC CCCTGGAAGA GGCGCCTGCT 
GTGCTGGACA AGCAGGACAT TGTGTATGTG GTAGAGGGCC AGCCTGCCTG TGTCACCGTC 
ACTTTTAACC ATGTGGAGGC CCAGGTTGTC TGGAGGAGCT GCCGGGGAGC CCTCCTCGAG 
GCGCGCACAG GTGTGTATGA ACTGAGTCAG CCAGATGACG ACCAGTATTG TCTGCGGATC 
TGCCGGGTGA GCCGACGAGA CCTGGGACCC CTTACTTGCA GTGCCCGAAA CCGCCACGGC 
ACAAAGGCCT GCTCGGTCAC CCTGGAGCTG GCAGAGGCAC CTCGGTTTGA GTCTATCATG 
GAAGATGTGG AGGTGGGGCC TGGAGAAACC GCTCGCTTTG CTGTGGTAGT TGAGGGGAAA 
CCACTGCCAG ACATCATGTG GTACAAGGAT GAGGTGCTGC TGGCCGAGAG CAACCATGTG 
AGCTTCGTGT ATGAGGAGAA CGAGTGTTCC CTAGTGCTGC TCAGCGCAGG AAGCCAGGAT 
GGAGGCGTCT ATACCTGTAC CGCCCGGAAC CTGGCAGGCG AAGTCTCCTG CAAGGCGGAG 
CTGTCTGTGC TTTCAGCTCA GACAGCCATG GAGGTCGAGG GGGTCGGAGA GGATGAGGAA 
CACCGAGGAA GAAGACTTAG CGACTACTAC GACATCCACC AAGAGATTGG CAGGGGTGCC 
TTCTCCTACC TGCGGCGTGT AGTGGAGAGG AGCTCTGGCC TGGAGTTTGC AGCCAAGTTC 
ATCCCTAGCC AGGCTAAGCC TAAAGCATCA GCAAGACGCG AGGCGCGCTT GCTGGCCCGG 
CTCCAGCATG GCTGTGTGCT CTACTTCCAC GAAGCCTTTG AGAGGCGCAG GGGACTTGTC 
ATAGTCACGG AGCTCTGCAC AGAAGAGCTT CTAGAGCGCA TGGCCAGGAA GCCCACCGTG 
TGTGAGTCTG AGACTCGGAC CTATATGCGT CAAGTGCTAG AGGGGATATG CTATCTGCAT 
CAGAGCCATG TGCTGCACCT GGATGTCAAG CCGGAGAACC TGCTTGTGTG GGATGGTGCA 
GGGGGTGAAG AGCAGGTGCG CATCTGTGAC TTCGGGAACG CCCAGGAACT CACTCCGGGA 
GAGCCCCAGT ACTGTCAGTA TGGCACGCCA GAGTTTGTAG CACCTGAGAT TGTCAACCAG 
AGCCCTGTGT CTGGAGTCAC TGACATCTGG CCCGTGGGCG TCGTTGCCTT TCTCTGTTTG 
ACGGGCATCT CCCCATTTGT TGGGGAGAAT GACCGGACAA CACTCATGAA TATCCGAAAC 
TACAACGTGG CATTTGAGGA GACTACGTTC CTGAGTCTGA GCAGGGAGGC CCGGGGCTTC 
CTCATCAAAG TGCTGGTGCA GGATCGACTG AGACCTACTG CAGAAGAGAC ATTGGAACAT 
CCTTGGTTCA AAACAGAAGC AAAGGGTGCA GAGGTGAGCA CAGATCACCT GAAGCTCTTC 
CTGTCACGGA GGAGGTGGCA GCGCTCTCAA ATCAGCTACA AGTGCCACCT GGTGCTGCGC 
CCCATTCCGG AACTGCTACG GGCTCCCCCG GAGCGGGTGT GGGTGGCCAT GCCTAGAAGA 
CAACCCCCGA GTGGGGGACT CTCATCCTCT TCAGACTCAG AAGAGGAAGA GTTGGAGGAA 
CTTCCCTCAG TACCCCGCCC CCTGCAGCCT GAGTTCTCAG GTTCTCGGGT ATCCCTCACC 
GACATCCCCA CCGAAGATGA GGCTCTGGGG ACCCCGGAAG CTGGGGCTGC CACCCCCATG 
GACTGGCAAG AGCAGGAAAG GACTCCCTCT AAGGACCAGG AGGCCCCCAG CCCTGAGGCA 
CTCCCCTCTC CAGGCCAGGA GTCCCCTGAT GGGCCTAGCC CCAGGCGGCC AGAGCTCCGC 
AGGGGTAGCT CTGCTGAGAG TGCCCTGCCC CGAGTCGGGT CGCGGGAACC GGGCCGGAGC 
TTGCACAAGG CAGCATCCGT GGAGCTGCCG CAGCGCAGGA GCCCCAGCCC GGGTGCCACC 
CGCCTGACTC GGGGAGGCCT GGGTGAGGGC GAGTATGCCC AGAGGCTGCA GGCCCTGCGT 
CAGCGGCTGC TGAGGGGAGG CCCCGAGGAT GGCAAGGTCA GCGGCCTCAG GGGACCCCTG 
CTGGAGAGCC TGGGGGGCCG TGCACGGGAT CCCAGGATGG CAAGGGCTGC CTCCAGCGAG 
GCTGCCCCCC ACCACCAGCC CCCTCCGGAG TCCCGGGGTC TGCAAAAGAG CAGCAGCTTC 
TCTCAGGGCG AGGCAGAGCC CCGGGGTCGG CACCGCCGCG CGGGGGCACC CCTCGAAATC 
CCCGTGGCCA GACTTGGGGC TCGCAGGCTG CAGGAGTCTC CCTCCTTATC TGCCCTTAGC 
GAGACCCAGC CACCGAGCCC TGCGAGGCCC AGCGTCCCCA AACTCAGTAT CACCAAGTCC 
CCGGAACCTT CCGCTGTCAC ATCCAGAGAC TCTCCCCAGC CCCCGGAACC TCAGCCTGTC 
CCAGAAAAGG TCCCTGAGCC CAAACCAGAA CCAGTCCGAG CTGCAAAACC AGCACAACCC 
CCTTTGGCTC TGCAAATGCC AACGCAGCCT CTCACCCCCT ATGCCCAGAT CATGCAGTCG 
CTCCAGCTGT CCAGTCCCAC CCTGTCCCCA CAGGACCCCG CTGTGCCTCC ATCAGAGCCC 
AAGCCCCACG CGGCTGTGTT TGCCAGGGTG GCTTCCCCAC CACCCGGAGT CTCTGAGAAG 
CGCGTGCCTT CGGCCAGAAC TCCCCCAGTG CTAGCCGAGA AAGCCCGAGT CCCCACGGTG 
CCCCCCAGGC CAGGCAGCAG CCTCAGCGGC AGCATCGAAA ACCTTGAATC AGAGGCTGTG 
TTCGAGGCTA AGTTCAAACG CAGCCGCGAA TCGCCACTGT CGCGGGGCCT GAGGCTACTG 
AGCCGCTCGC GCTCGGAGGA GCGCGGTCCC TTCCGTGGGG CGGAGGACGA CGGCATATAC 
CGGCCCAGTC CGGCAGGAAC GCCGCTAGAG CTGGTGCGAC GGCCCGAGCG CTCGCGCTCC 
GTGCAGGACC TCAGGGTAGC CGGAGAGCCG GGCCTTGTGC GTCGCCTTTC GCTGTCCCTG 
TCGCAGAAGC TGCGGCGGAC ACCGCCGGGA CAGCGACACC CTGCCTGGGA GTCCCGCAGC 
GGAGACGGGG AGAGCTCCGA GGGCGGCAGC TCAGCTCGGG CATCCCCAGT GCTGGCGGTA 
CGCAGGAGAC TCAGCTCCAC GCTGGAGCGT CTGTCCAGCC GTTTGCAGCG CAGCGGCAGC 
AGCGAGGACT CCGGGGGCGC CTCGGGTCGC AGCACGCCGC TGTTTGGACG GCTGCGCAGG 
GCTACTTCGG AAGGGGAGAG CCTGCGACGC CTCGGTGTTC CGCACAATCA GCTGGGCTCT 
CAGACTGGTG CCACTACACC TTCCGCTGAG TCTCTGGGCT CAGAGGCCAG TGGCACATCG 
GGTTCTTCAG CTCCTGGAGA AAGCCGAAGC CGGCACCGGT GGGGCCTTTC CAGGCTGCGC 
AAGGACAAGG GATTATCTCA GCCAAACCTC TCCTCCAGTG TCCAAGAGGA CTTGGGTCAC 
CAGTATGTGC CCAGTGAGTC AGATTTCCCC CCAGTCTTCC ACATCAAACT CAAGGACCAG 
GTGCTGTTGG AGGGGGAAGC AGCCACCCTG CTCTGCCTAC CCGCAGCCTG CCCAGCACCC 
CGCATCTCCT GGATGAAAGA TAAGCAGTCC CTGCGTTCAG AGCCCTCAGT GGTCATTGTG 
TCCTGCAAAG ATGGACGGCA GCTGCTGAGC ATACCCCGGG CCGGCAAGCG GCATGCTGGG 
CTCTATGAGT GCTCAGCCAC CAATGTCCTG GGCAGCATTA CCAGCTCCTG TACTGTGGCC 
GTGGCCCGCA TTCCAGGGAA GCTAGCTCCT CCAGAGGTGC CCCAGACCTA CCATGACACA 
GCACTAGTAG TGTGGAAGCC AGGAGACGGT CGGGCACCTT GCACATACAC TCTGGAGCGG 
CGGGTGGATG GGGAATCTGT CTGGCACCCT GTGAGCTCAG GCATCCCCGA CTGTTATTAC 
AATGTGACTC AGCTGCCTGT CGGCGTGACC GTGCGGTTCC GTGTGGCCTG TTCCAATCGT 
GCTGGGCAGG GGCCCTTCAG CAACCCTTCT GAGAAGGTCT TCATCCGAGG CACTCCAGAT 
TCTCCTGCTC AGCCAGCTGC TGCTCCCCGA GATGCCCCTG TTACCTCAGG GCCAACCAGG 
GCCCCACCGC CTGACTCTCC TACCTCACTG GCCCCCACCC CCGCTCTTGC GCCCCCAGCC 
TCCCAGGCAT CCACTCTCAG CCCCTCAACT TCCTCCATGT CTGCCAACCA GGCCTTGTCC 
TCACTCAAGG CTGTGGGTCC ACCACCCGCA ACCCCTCCAC GAAAGCACAG GGGCCTTCTG 
GCCACCCAGC AAGCAGAGCC ATCCCCACCC AGTATCGTGG TCACCCCAAG TGAACCCAGG 
TCTTTTGTCC CTGACACTGG AACCCTGACC CCAACCTCTA GTCCTCAAGG GGTTAAACCA 
GCACCTTCCT CAACTTCCTT GTATATGGTG ACTTCCTTCG TGTCTGCACC TCCAGCCCCC 
CAGGCCCCAG CCCCTGAGCC CCCTCCTGAG CCCACCAAGG TGACTGTGCG GAGCCTCAGC 
CCAGCCAAGG AGGTGGTCAG CTCCCCAACT CCTGAGAGCA CCACTCTTCG GCAGGGTCCC 
CCTCAGAAAC CCTACACCTT CCTGGAGGAG AAGGCCAGGG GCCGCTTTGG CGTTGTGCGG 
TCATGCCGGG AGAATGCTAC GGGCCGAACG TTTGTCGCCA AGATCGTGCC GTACGCCGCG 
GAGGGCAAGC GACGCGTGCT GCAGGAATAC GAGGTGCTTC GGACGCTGCA CCACGAGCGG 
CTCATGTCCC TGCACGAGGC CTACATCACC CCTCGGTACC TCGTGCTTAT CGCTGAGAGC 
TGTGGCAACC GGGAACTCCT CTGTGGGCTC AGCGACAGAT TCCGGTATTC AGAAGATGAC 
GTGGCCACCT ATGTGGTGCA GCTGCTACAA GGCCTGGACT ACCTCCACGG CCACCACGTG 
CTACACCTGG ACATCAAGCC GGACAATCTA CTGTTGGCCG CTGACAATGC CCTTAAAATT 
GTGGACTTCG GCAGTGCTCA GCCCTATAAC CCTCAGGCCC TGAAGCCACT TGGCCACCGC 
ACAGGCACTC TGGAGTTCAT GGCTCCTGAG ATGGTGAAGG GAGACCCCAT TGGCTCTGCC 
ACGGACATCT GGGGAGCGGG TGTGCTCACC TACATCATGC TTAGTGGGTA CTCCCCATTC 
TATGAGCCAG ACCCCCAGGA AACAGAGGCT CGGATTGTTG GGGGTCGCTT TGATGCCTTC 
CAGTTGTATC CTAACACATC CCAGAGTGCC ACCCTCTTCT TGAGAAAGGT CCTCTCAGTA 
CATCCCTGGA GCCGGCCCTC TCTGCAGGAC TGCTTGGCCC ACCCATGGCT GCAAGATGCC 
TACCTGATGA AGCTGCGCCG CCAGACACTC ACCTTCACCA CCAACCGGCT CAAGGAATTC 
CTGGGCGAGC AGCGGCGACG TCGGGCTGAG GCTGCTACCC GTCACAAGGT GCTGCTCCGC 
TCCTACCCTG GCAGCCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_011238646.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236948.1 Mus musculus SPEG complex locus (Speg), transcript variant X5, mRNA.

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CloneID	OMu67147
Clone ID Related Accession (Same CDS sequence)	XM_011238646.1 , XM_011238646.2
Accession Version	XM_011238646.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGACTC GGAGACTGCC GAGGATGACA TCAGCGATGT GCCGGGGACC 
CAGCGCCTGG AGCTTCGGGA TGACAGGGCC TTCAGCACCC CCACGGGGGG CTCTGATACC 
CTGGTGGGTA CCTCCCTGGA CACACCCCCG ACCTCCGTGA CAGGCACCTC AGAGGAGCAA 
GTGAGCTGGT GGGGCAGCGG GCAGACGGTC CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC 
ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA GCACAGACAA CCGGGGCCGG GCCACGGCAC 
CTGGGGGTGG AGCCGCTGGT GCGGGCATCG CGAGCTAATC TGGTGGGCGC AAGCTGGGGG 
TCAGAGGATA GCCTTTCGGT GGCCAGTGAC CTCTATGGCA GCGCGTTCAG TCTGTACAGA 
GGACGGGCGC TCTCGATCCA CGTCAGCATT CCTCCGAGTG GATTGCACAG AGAGGAGCCT 
GACCTTCAGC CTCAGCCAGC CAGTGATGCC CTTCGTCCAC GCCCTGCCCT TCCACCTCCC 
TCCAAATCCG CTTTGCTTCC CCCACCATCT CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA 
CCTAGTACCC AGCCCCCAGC TACTCCCACT TCGCCCCATC GGCGTGCTCA GGAGCCCTCG 
CTGCCTGAGG ACATCACCAC CACGGAAGAG AAGCGAGGGA AAAAGCCCAA GTCATCCGGG 
CCGTCGCTGG CGGGCACAGT GGAGTCCCGG CCCCAGACGC CACTGAGCGA GGCTTCGGGT 
CGCCTGTCAG CATTGGGCCG CTCGCCCCGG CTGGTGCGCG CGGGGTCCCG CATCCTGGAC 
AAGCTACAGT TCTTCGAAGA GCGGCGACGC AGCCTGGAGC GCAGCGACTC GCCGCCAGCG 
CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG 
GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC 
AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG CAAAAGGCGG CCTCGTTGGA TGAACGCACG 
CGACAACGCA GTGCAACCTC GGACCTCGAA CTCCGCTTCG CCCAGGAGCT GGGTCGCATC 
CGCCGATCTA CGTCGCGGGA GGAGCTGGTG CGTTCGCACG AGTCCCTGCG TGCCACGCTG 
CAGCGCGCCC CATCCCCTCG GGAGCCCGGC GAGCCCCCAC TCTTCTCCCG GCCTTCCACA 
CCCAAGACCT CACGGGCTGT GAGCCCGGCT GCCACCCAGC CGCCGCCTCC TAGTGGTGCG 
GGCAAATCTG GGGACGAGCC TGGGAGGCCC CGAAGCAGAG GGCCGGTGGG CAGGACTGAA 
CCGGGGGAAG GCCCGCAGCA GGAGATCAAG CGTCGGGACC AATTCCCGCT AACCAGGAGC 
AGAGCCATCC AGGAGTGCAG GAGCCCTGTG CCGCCCTACA CCGCGGATCC CCCGGAGAGC 
AGGACAAAAG CCCCCTCCGG TCGCAAGCGG GAACCCCCTG CTCAAGCGGT GCGCTTTCTG 
CCCTGGGCCA CTCCGGGAGT GGAGGACTCT GTTCTGCCCC AAACCTTGGA GAAGAATAGA 
GCGGGACCCG AGGCTGAGAA GAGGCTTCGC AGAGGACCTG AGGAGGATGG CCCCTGGGGG 
CCCTGGGACC GCAGAGGGAC CCGCAGCCAA GGCAAAGGTC GCCGTGCTCG GCCTACTTCC 
CCCGAGCTCG AGTCCTCAGA CGACTCCTAT GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA 
CCCGTGTTTG AGATCCCTCT GCAGAATATG GTGGTGGCGC CAGGAGCTGA CGTGCTACTT 
AAGTGTATCA TCACCGCCAA CCCCCCACCC CAAGTGTCCT GGAAAAAGGA TGGGTCCATG 
TTGCACAGCG AGGGTCGTCT TCTCATCCGG GCTGAAGGTG AACGGCACAC ACTGCTGCTC 
AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC TACACAGCCA CTGCCACCAA CGAACTGGGC 
CAAGCTACCT GTGCTTCTTC ACTGGCTGTG AGACCTGGCG GCTCCACATC CCCTTTCAGC 
AGCCCCATCA CCTCTGATGA GGAGTACCTG AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG 
GAGACCTGGC CCCGAACCCC TACCATGAAG CTCAGTCCCA GCCAGGATCA TGATTCCTCC 
GACTCTTCTT CCAAGGCACC CCCAACGTTC AAGGTCTCAC TCATGGACCA ATCGGTGAGA 
GAAGGTCAAG ATGTCATTAT GAGCATCCGT GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC 
TGGCTGAGGA ATCGACAGCC CGTGCGCCCA GACCAGCGGC GCTTTGCAGA GGAGGCCGAG 
GGTGGGCTCT GCCGCTTGAG GATCCTGGCT GCTGAACGGG GCGATGCTGG TTTCTACACA 
TGCAAGGCGG TCAACGAATA TGGCGCTCGG CAGTGCGAGG CCCGCCTGGA GGTCCGAGCA 
CACCCTGAAA GCCGGTCCCT GGCCGTGCTG GCCCCCCTGC AGGACGTGGA CGTGGGGGCC 
GGGGAGATGG CGCTGTTTGA GTGCCTGGTG GCAGGTCCAG CTGACGTCGA GGTGGACTGG 
CTGTGCCGGG GCCGCCTGCT GCAGCCTGCG CTGCTCAAAT GCAAGATGCA TTTTGATGGC 
CGCAAGTGCA AGCTGCTGCT CACCTCTGTG CACGAGGACG ACAGTGGTGT CTACACCTGC 
AAGCTGAGCA CAGCCAAAGA TGAACTGACC TGCAGTGCCC GGCTGACGGT TCGGCCGTCG 
CTGGCGCCTC TCTTCACCCG GTTGCTGGAA GATGTGGAGG TGTTGGAAGG CCGTGCTGCC 
CGCTTGGATT GCAAGATCAG CGGTACCCCG CCTCCCTCCG TTACCTGGAC TCATTTTGGC 
CACCCCGTGA ATGAGGGTGA CAACTTGCGG CTTCAACAGG ATGGAGGTCT ACACTCACTG 
CACATTGCCC GGGTGGGCAG TGAGGACGAA GGACTCTATG AAGTCAGTGC CACCAACACT 
CATGGCCAGG CCCACTGCTC CGCCCAGCTG TATGTGGAGG AGCCTCGGAC AGCTGCCTCT 
GGCCCCAGCT CGAAGCTGGA GAAGATGCCA TCTATCCCTG AGGAGCCGGA GCACGGAGAT 
CTAGAGAGGC TGTCTATTCC TGACTTCCTA CGGCCACTGC AAGATCTGGA AGTGGGACTA 
GCTAAGGAGG CCATGCTGGA GTGCCAAGTG ACCGGCCTGC CCTATCCCAC CATCAGCTGG 
TTCCACAATG GCCACCGCAT TCAAAGCAGT GACGACCGAC GCATGACACA GTACAGGGAC 
ATACATCGCC TGGTGTTCCC AGCTGTGGGG CCTCAGCATG CTGGTGTCTA TAAGAGTGTA 
ATCGCCAACA AGCTGGGCAA AGCGGCCTGC TACGCCCATC TCTATGTCAC AGATGTGGTT 
CCAGGCCCTC CAGATGGTGC TCCGGAAGTA GTAGCTGTGA CCGGAAGGAT GGTCACACTA 
TCGTGGAACC CACCCAGGAG TCTGGACATG GCTATTGACC CAGATTCCCT AACATACACT 
GTGCAGCACC AGGTGTTGGG CTCGGACCAG TGGACGGCAC TGGTCACAGG CCTGCGGGAG 
CCTGCGTGGG CAGCCACAGG GCTGAAGAAG GGGATCCAGC ACATCTTCCG GGTCCTCAGC 
TCCAGTGGCA AGAGCAGCAG CAAGCCCTCA GCACCGTCGG AACCCGTGCA GCTGCTAGAG 
CATGGCCCAC CCCTGGAAGA GGCGCCTGCT GTGCTGGACA AGCAGGACAT TGTGTATGTG 
GTAGAGGGCC AGCCTGCCTG TGTCACCGTC ACTTTTAACC ATGTGGAGGC CCAGGTTGTC 
TGGAGGAGCT GCCGGGGAGC CCTCCTCGAG GCGCGCACAG GTGTGTATGA ACTGAGTCAG 
CCAGATGACG ACCAGTATTG TCTGCGGATC TGCCGGGTGA GCCGACGAGA CCTGGGACCC 
CTTACTTGCA GTGCCCGAAA CCGCCACGGC ACAAAGGCCT GCTCGGTCAC CCTGGAGCTG 
GCAGAGGCAC CTCGGTTTGA GTCTATCATG GAAGATGTGG AGGTGGGGCC TGGAGAAACC 
GCTCGCTTTG CTGTGGTAGT TGAGGGGAAA CCACTGCCAG ACATCATGTG GTACAAGGAT 
GAGGTGCTGC TGGCCGAGAG CAACCATGTG AGCTTCGTGT ATGAGGAGAA CGAGTGTTCC 
CTAGTGCTGC TCAGCGCAGG AAGCCAGGAT GGAGGCGTCT ATACCTGTAC CGCCCGGAAC 
CTGGCAGGCG AAGTCTCCTG CAAGGCGGAG CTGTCTGTGC TTTCAGCTCA GACAGCCATG 
GAGGTCGAGG GGGTCGGAGA GGATGAGGAA CACCGAGGAA GAAGACTTAG CGACTACTAC 
GACATCCACC AAGAGATTGG CAGGGGTGCC TTCTCCTACC TGCGGCGTGT AGTGGAGAGG 
AGCTCTGGCC TGGAGTTTGC AGCCAAGTTC ATCCCTAGCC AGGCTAAGCC TAAAGCATCA 
GCAAGACGCG AGGCGCGCTT GCTGGCCCGG CTCCAGCATG GCTGTGTGCT CTACTTCCAC 
GAAGCCTTTG AGAGGCGCAG GGGACTTGTC ATAGTCACGG AGCTCTGCAC AGAAGAGCTT 
CTAGAGCGCA TGGCCAGGAA GCCCACCGTG TGTGAGTCTG AGACTCGGAC CTATATGCGT 
CAAGTGCTAG AGGGGATATG CTATCTGCAT CAGAGCCATG TGCTGCACCT GGATGTCAAG 
CCGGAGAACC TGCTTGTGTG GGATGGTGCA GGGGGTGAAG AGCAGGTGCG CATCTGTGAC 
TTCGGGAACG CCCAGGAACT CACTCCGGGA GAGCCCCAGT ACTGTCAGTA TGGCACGCCA 
GAGTTTGTAG CACCTGAGAT TGTCAACCAG AGCCCTGTGT CTGGAGTCAC TGACATCTGG 
CCCGTGGGCG TCGTTGCCTT TCTCTGTTTG ACGGGCATCT CCCCATTTGT TGGGGAGAAT 
GACCGGACAA CACTCATGAA TATCCGAAAC TACAACGTGG CATTTGAGGA GACTACGTTC 
CTGAGTCTGA GCAGGGAGGC CCGGGGCTTC CTCATCAAAG TGCTGGTGCA GGATCGACTG 
AGACCTACTG CAGAAGAGAC ATTGGAACAT CCTTGGTTCA AAACAGAAGC AAAGGGTGCA 
GAGGTGAGCA CAGATCACCT GAAGCTCTTC CTGTCACGGA GGAGGTGGCA GCGCTCTCAA 
ATCAGCTACA AGTGCCACCT GGTGCTGCGC CCCATTCCGG AACTGCTACG GGCTCCCCCG 
GAGCGGGTGT GGGTGGCCAT GCCTAGAAGA CAACCCCCGA GTGGGGGACT CTCATCCTCT 
TCAGACTCAG AAGAGGAAGA GTTGGAGGAA CTTCCCTCAG TACCCCGCCC CCTGCAGCCT 
GAGTTCTCAG GTTCTCGGGT ATCCCTCACC GACATCCCCA CCGAAGATGA GGCTCTGGGG 
ACCCCGGAAG CTGGGGCTGC CACCCCCATG GACTGGCAAG AGCAGGAAAG GACTCCCTCT 
AAGGACCAGG AGGCCCCCAG CCCTGAGGCA CTCCCCTCTC CAGGCCAGGA GTCCCCTGAT 
GGGCCTAGCC CCAGGCGGCC AGAGCTCCGC AGGGGTAGCT CTGCTGAGAG TGCCCTGCCC 
CGAGTCGGGT CGCGGGAACC GGGCCGGAGC TTGCACAAGG CAGCATCCGT GGAGCTGCCG 
CAGCGCAGGA GCCCCAGCCC GGGTGCCACC CGCCTGACTC GGGGAGGCCT GGGTGAGGGC 
GAGTATGCCC AGAGGCTGCA GGCCCTGCGT CAGCGGCTGC TGAGGGGAGG CCCCGAGGAT 
GGCAAGGTCA GCGGCCTCAG GGGACCCCTG CTGGAGAGCC TGGGGGGCCG TGCACGGGAT 
CCCAGGATGG CAAGGGCTGC CTCCAGCGAG GCTGCCCCCC ACCACCAGCC CCCTCCGGAG 
TCCCGGGGTC TGCAAAAGAG CAGCAGCTTC TCTCAGGGCG AGGCAGAGCC CCGGGGTCGG 
CACCGCCGCG CGGGGGCACC CCTCGAAATC CCCGTGGCCA GACTTGGGGC TCGCAGGCTG 
CAGGAGTCTC CCTCCTTATC TGCCCTTAGC GAGACCCAGC CACCGAGCCC TGCGAGGCCC 
AGCGTCCCCA AACTCAGTAT CACCAAGTCC CCGGAACCTT CCGCTGTCAC ATCCAGAGAC 
TCTCCCCAGC CCCCGGAACC TCAGCCTGTC CCAGAAAAGG TCCCTGAGCC CAAACCAGAA 
CCAGTCCGAG CTGCAAAACC AGCACAACCC CCTTTGGCTC TGCAAATGCC AACGCAGCCT 
CTCACCCCCT ATGCCCAGAT CATGCAGTCG CTCCAGCTGT CCAGTCCCAC CCTGTCCCCA 
CAGGACCCCG CTGTGCCTCC ATCAGAGCCC AAGCCCCACG CGGCTGTGTT TGCCAGGGTG 
GCTTCCCCAC CACCCGGAGT CTCTGAGAAG CGCGTGCCTT CGGCCAGAAC TCCCCCAGTG 
CTAGCCGAGA AAGCCCGAGT CCCCACGGTG CCCCCCAGGC CAGGCAGCAG CCTCAGCGGC 
AGCATCGAAA ACCTTGAATC AGAGGCTGTG TTCGAGGCTA AGTTCAAACG CAGCCGCGAA 
TCGCCACTGT CGCGGGGCCT GAGGCTACTG AGCCGCTCGC GCTCGGAGGA GCGCGGTCCC 
TTCCGTGGGG CGGAGGACGA CGGCATATAC CGGCCCAGTC CGGCAGGAAC GCCGCTAGAG 
CTGGTGCGAC GGCCCGAGCG CTCGCGCTCC GTGCAGGACC TCAGGGTAGC CGGAGAGCCG 
GGCCTTGTGC GTCGCCTTTC GCTGTCCCTG TCGCAGAAGC TGCGGCGGAC ACCGCCGGGA 
CAGCGACACC CTGCCTGGGA GTCCCGCAGC GGAGACGGGG AGAGCTCCGA GGGCGGCAGC 
TCAGCTCGGG CATCCCCAGT GCTGGCGGTA CGCAGGAGAC TCAGCTCCAC GCTGGAGCGT 
CTGTCCAGCC GTTTGCAGCG CAGCGGCAGC AGCGAGGACT CCGGGGGCGC CTCGGGTCGC 
AGCACGCCGC TGTTTGGACG GCTGCGCAGG GCTACTTCGG AAGGGGAGAG CCTGCGACGC 
CTCGGTGTTC CGCACAATCA GCTGGGCTCT CAGACTGGTG CCACTACACC TTCCGCTGAG 
TCTCTGGGCT CAGAGGCCAG TGGCACATCG GGTTCTTCAG CTCCTGGAGA AAGCCGAAGC 
CGGCACCGGT GGGGCCTTTC CAGGCTGCGC AAGGACAAGG GATTATCTCA GCCAAACCTC 
TCCTCCAGTG TCCAAGAGGA CTTGGGTCAC CAGTATGTGC CCAGTGAGTC AGATTTCCCC 
CCAGTCTTCC ACATCAAACT CAAGGACCAG GTGCTGTTGG AGGGGGAAGC AGCCACCCTG 
CTCTGCCTAC CCGCAGCCTG CCCAGCACCC CGCATCTCCT GGATGAAAGA TAAGCAGTCC 
CTGCGTTCAG AGCCCTCAGT GGTCATTGTG TCCTGCAAAG ATGGACGGCA GCTGCTGAGC 
ATACCCCGGG CCGGCAAGCG GCATGCTGGG CTCTATGAGT GCTCAGCCAC CAATGTCCTG 
GGCAGCATTA CCAGCTCCTG TACTGTGGCC GTGGCCCGCA TTCCAGGGAA GCTAGCTCCT 
CCAGAGGTGC CCCAGACCTA CCATGACACA GCACTAGTAG TGTGGAAGCC AGGAGACGGT 
CGGGCACCTT GCACATACAC TCTGGAGCGG CGGGTGGATG GGGAATCTGT CTGGCACCCT 
GTGAGCTCAG GCATCCCCGA CTGTTATTAC AATGTGACTC AGCTGCCTGT CGGCGTGACC 
GTGCGGTTCC GTGTGGCCTG TTCCAATCGT GCTGGGCAGG GGCCCTTCAG CAACCCTTCT 
GAGAAGGTCT TCATCCGAGG CACTCCAGAT TCTCCTGCTC AGCCAGCTGC TGCTCCCCGA 
GATGCCCCTG TTACCTCAGG GCCAACCAGG GCCCCACCGC CTGACTCTCC TACCTCACTG 
GCCCCCACCC CCGCTCTTGC GCCCCCAGCC TCCCAGGCAT CCACTCTCAG CCCCTCAACT 
TCCTCCATGT CTGCCAACCA GGCCTTGTCC TCACTCAAGG CTGTGGGTCC ACCACCCGCA 
ACCCCTCCAC GAAAGCACAG GGGCCTTCTG GCCACCCAGC AAGCAGAGCC ATCCCCACCC 
AGTATCGTGG TCACCCCAAG TGAACCCAGG TCTTTTGTCC CTGACACTGG AACCCTGACC 
CCAACCTCTA GTCCTCAAGG GGTTAAACCA GCACCTTCCT CAACTTCCTT GTATATGGTG 
ACTTCCTTCG TGTCTGCACC TCCAGCCCCC CAGGCCCCAG CCCCTGAGCC CCCTCCTGAG 
CCCACCAAGG TGACTGTGCG GAGCCTCAGC CCAGCCAAGG AGGTGGTCAG CTCCCCAACT 
CCTGAGAGCA CCACTCTTCG GCAGGGTCCC CCTCAGAAAC CCTACACCTT CCTGGAGGAG 
AAGGCCAGGG GCCGCTTTGG CGTTGTGCGG TCATGCCGGG AGAATGCTAC GGGCCGAACG 
TTTGTCGCCA AGATCGTGCC GTACGCCGCG GAGGGCAAGC GACGCGTGCT GCAGGAATAC 
GAGGTGCTTC GGACGCTGCA CCACGAGCGG CTCATGTCCC TGCACGAGGC CTACATCACC 
CCTCGGTACC TCGTGCTTAT CGCTGAGAGC TGTGGCAACC GGGAACTCCT CTGTGGGCTC 
AGCGACAGAT TCCGGTATTC AGAAGATGAC GTGGCCACCT ATGTGGTGCA GCTGCTACAA 
GGCCTGGACT ACCTCCACGG CCACCACGTG CTACACCTGG ACATCAAGCC GGACAATCTA 
CTGTTGGCCG CTGACAATGC CCTTAAAATT GTGGACTTCG GCAGTGCTCA GCCCTATAAC 
CCTCAGGCCC TGAAGCCACT TGGCCACCGC ACAGGCACTC TGGAGTTCAT GGCTCCTGAG 
ATGGTGAAGG GAGACCCCAT TGGCTCTGCC ACGGACATCT GGGGAGCGGG TGTGCTCACC 
TACATCATGC TTAGTGGGTA CTCCCCATTC TATGAGCCAG ACCCCCAGGA AACAGAGGCT 
CGGATTGTTG GGGGTCGCTT TGATGCCTTC CAGTTGTATC CTAACACATC CCAGAGTGCC 
ACCCTCTTCT TGAGAAAGGT CCTCTCAGTA CATCCCTGGA GCCGGCCCTC TCTGCAGGAC 
TGCTTGGCCC ACCCATGGCT GCAAGATGCC TACCTGATGA AGCTGCGCCG CCAGACACTC 
ACCTTCACCA CCAACCGGCT CAAGGAATTC CTGGGCGAGC AGCGGCGACG TCGGGCTGAG 
GCTGCTACCC GTCACAAGGT GCTGCTCCGC TCCTACCCTG GCAGCCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236948.1
CDS	65..9535
Translation	MKKLWVKKRFQKTGHSRRAFGRLTHDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPRQAQTTGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_011238646.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238646.1

ORF Insert Sequence:

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGACTC GGAGACTGCC GAGGATGACA TCAGCGATGT GCCGGGGACC 
CAGCGCCTGG AGCTTCGGGA TGACAGGGCC TTCAGCACCC CCACGGGGGG CTCTGATACC 
CTGGTGGGTA CCTCCCTGGA CACACCCCCG ACCTCCGTGA CAGGCACCTC AGAGGAGCAA 
GTGAGCTGGT GGGGCAGCGG GCAGACGGTC CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC 
ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA GCACAGACAA CCGGGGCCGG GCCACGGCAC 
CTGGGGGTGG AGCCGCTGGT GCGGGCATCG CGAGCTAATC TGGTGGGCGC AAGCTGGGGG 
TCAGAGGATA GCCTTTCGGT GGCCAGTGAC CTCTATGGCA GCGCGTTCAG TCTGTACAGA 
GGACGGGCGC TCTCGATCCA CGTCAGCATT CCTCCGAGTG GATTGCACAG AGAGGAGCCT 
GACCTTCAGC CTCAGCCAGC CAGTGATGCC CTTCGTCCAC GCCCTGCCCT TCCACCTCCC 
TCCAAATCCG CTTTGCTTCC CCCACCATCT CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA 
CCTAGTACCC AGCCCCCAGC TACTCCCACT TCGCCCCATC GGCGTGCTCA GGAGCCCTCG 
CTGCCTGAGG ACATCACCAC CACGGAAGAG AAGCGAGGGA AAAAGCCCAA GTCATCCGGG 
CCGTCGCTGG CGGGCACAGT GGAGTCCCGG CCCCAGACGC CACTGAGCGA GGCTTCGGGT 
CGCCTGTCAG CATTGGGCCG CTCGCCCCGG CTGGTGCGCG CGGGGTCCCG CATCCTGGAC 
AAGCTACAGT TCTTCGAAGA GCGGCGACGC AGCCTGGAGC GCAGCGACTC GCCGCCAGCG 
CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG 
GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC 
AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG CAAAAGGCGG CCTCGTTGGA TGAACGCACG 
CGACAACGCA GTGCAACCTC GGACCTCGAA CTCCGCTTCG CCCAGGAGCT GGGTCGCATC 
CGCCGATCTA CGTCGCGGGA GGAGCTGGTG CGTTCGCACG AGTCCCTGCG TGCCACGCTG 
CAGCGCGCCC CATCCCCTCG GGAGCCCGGC GAGCCCCCAC TCTTCTCCCG GCCTTCCACA 
CCCAAGACCT CACGGGCTGT GAGCCCGGCT GCCACCCAGC CGCCGCCTCC TAGTGGTGCG 
GGCAAATCTG GGGACGAGCC TGGGAGGCCC CGAAGCAGAG GGCCGGTGGG CAGGACTGAA 
CCGGGGGAAG GCCCGCAGCA GGAGATCAAG CGTCGGGACC AATTCCCGCT AACCAGGAGC 
AGAGCCATCC AGGAGTGCAG GAGCCCTGTG CCGCCCTACA CCGCGGATCC CCCGGAGAGC 
AGGACAAAAG CCCCCTCCGG TCGCAAGCGG GAACCCCCTG CTCAAGCGGT GCGCTTTCTG 
CCCTGGGCCA CTCCGGGAGT GGAGGACTCT GTTCTGCCCC AAACCTTGGA GAAGAATAGA 
GCGGGACCCG AGGCTGAGAA GAGGCTTCGC AGAGGACCTG AGGAGGATGG CCCCTGGGGG 
CCCTGGGACC GCAGAGGGAC CCGCAGCCAA GGCAAAGGTC GCCGTGCTCG GCCTACTTCC 
CCCGAGCTCG AGTCCTCAGA CGACTCCTAT GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA 
CCCGTGTTTG AGATCCCTCT GCAGAATATG GTGGTGGCGC CAGGAGCTGA CGTGCTACTT 
AAGTGTATCA TCACCGCCAA CCCCCCACCC CAAGTGTCCT GGAAAAAGGA TGGGTCCATG 
TTGCACAGCG AGGGTCGTCT TCTCATCCGG GCTGAAGGTG AACGGCACAC ACTGCTGCTC 
AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC TACACAGCCA CTGCCACCAA CGAACTGGGC 
CAAGCTACCT GTGCTTCTTC ACTGGCTGTG AGACCTGGCG GCTCCACATC CCCTTTCAGC 
AGCCCCATCA CCTCTGATGA GGAGTACCTG AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG 
GAGACCTGGC CCCGAACCCC TACCATGAAG CTCAGTCCCA GCCAGGATCA TGATTCCTCC 
GACTCTTCTT CCAAGGCACC CCCAACGTTC AAGGTCTCAC TCATGGACCA ATCGGTGAGA 
GAAGGTCAAG ATGTCATTAT GAGCATCCGT GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC 
TGGCTGAGGA ATCGACAGCC CGTGCGCCCA GACCAGCGGC GCTTTGCAGA GGAGGCCGAG 
GGTGGGCTCT GCCGCTTGAG GATCCTGGCT GCTGAACGGG GCGATGCTGG TTTCTACACA 
TGCAAGGCGG TCAACGAATA TGGCGCTCGG CAGTGCGAGG CCCGCCTGGA GGTCCGAGCA 
CACCCTGAAA GCCGGTCCCT GGCCGTGCTG GCCCCCCTGC AGGACGTGGA CGTGGGGGCC 
GGGGAGATGG CGCTGTTTGA GTGCCTGGTG GCAGGTCCAG CTGACGTCGA GGTGGACTGG 
CTGTGCCGGG GCCGCCTGCT GCAGCCTGCG CTGCTCAAAT GCAAGATGCA TTTTGATGGC 
CGCAAGTGCA AGCTGCTGCT CACCTCTGTG CACGAGGACG ACAGTGGTGT CTACACCTGC 
AAGCTGAGCA CAGCCAAAGA TGAACTGACC TGCAGTGCCC GGCTGACGGT TCGGCCGTCG 
CTGGCGCCTC TCTTCACCCG GTTGCTGGAA GATGTGGAGG TGTTGGAAGG CCGTGCTGCC 
CGCTTGGATT GCAAGATCAG CGGTACCCCG CCTCCCTCCG TTACCTGGAC TCATTTTGGC 
CACCCCGTGA ATGAGGGTGA CAACTTGCGG CTTCAACAGG ATGGAGGTCT ACACTCACTG 
CACATTGCCC GGGTGGGCAG TGAGGACGAA GGACTCTATG AAGTCAGTGC CACCAACACT 
CATGGCCAGG CCCACTGCTC CGCCCAGCTG TATGTGGAGG AGCCTCGGAC AGCTGCCTCT 
GGCCCCAGCT CGAAGCTGGA GAAGATGCCA TCTATCCCTG AGGAGCCGGA GCACGGAGAT 
CTAGAGAGGC TGTCTATTCC TGACTTCCTA CGGCCACTGC AAGATCTGGA AGTGGGACTA 
GCTAAGGAGG CCATGCTGGA GTGCCAAGTG ACCGGCCTGC CCTATCCCAC CATCAGCTGG 
TTCCACAATG GCCACCGCAT TCAAAGCAGT GACGACCGAC GCATGACACA GTACAGGGAC 
ATACATCGCC TGGTGTTCCC AGCTGTGGGG CCTCAGCATG CTGGTGTCTA TAAGAGTGTA 
ATCGCCAACA AGCTGGGCAA AGCGGCCTGC TACGCCCATC TCTATGTCAC AGATGTGGTT 
CCAGGCCCTC CAGATGGTGC TCCGGAAGTA GTAGCTGTGA CCGGAAGGAT GGTCACACTA 
TCGTGGAACC CACCCAGGAG TCTGGACATG GCTATTGACC CAGATTCCCT AACATACACT 
GTGCAGCACC AGGTGTTGGG CTCGGACCAG TGGACGGCAC TGGTCACAGG CCTGCGGGAG 
CCTGCGTGGG CAGCCACAGG GCTGAAGAAG GGGATCCAGC ACATCTTCCG GGTCCTCAGC 
TCCAGTGGCA AGAGCAGCAG CAAGCCCTCA GCACCGTCGG AACCCGTGCA GCTGCTAGAG 
CATGGCCCAC CCCTGGAAGA GGCGCCTGCT GTGCTGGACA AGCAGGACAT TGTGTATGTG 
GTAGAGGGCC AGCCTGCCTG TGTCACCGTC ACTTTTAACC ATGTGGAGGC CCAGGTTGTC 
TGGAGGAGCT GCCGGGGAGC CCTCCTCGAG GCGCGCACAG GTGTGTATGA ACTGAGTCAG 
CCAGATGACG ACCAGTATTG TCTGCGGATC TGCCGGGTGA GCCGACGAGA CCTGGGACCC 
CTTACTTGCA GTGCCCGAAA CCGCCACGGC ACAAAGGCCT GCTCGGTCAC CCTGGAGCTG 
GCAGAGGCAC CTCGGTTTGA GTCTATCATG GAAGATGTGG AGGTGGGGCC TGGAGAAACC 
GCTCGCTTTG CTGTGGTAGT TGAGGGGAAA CCACTGCCAG ACATCATGTG GTACAAGGAT 
GAGGTGCTGC TGGCCGAGAG CAACCATGTG AGCTTCGTGT ATGAGGAGAA CGAGTGTTCC 
CTAGTGCTGC TCAGCGCAGG AAGCCAGGAT GGAGGCGTCT ATACCTGTAC CGCCCGGAAC 
CTGGCAGGCG AAGTCTCCTG CAAGGCGGAG CTGTCTGTGC TTTCAGCTCA GACAGCCATG 
GAGGTCGAGG GGGTCGGAGA GGATGAGGAA CACCGAGGAA GAAGACTTAG CGACTACTAC 
GACATCCACC AAGAGATTGG CAGGGGTGCC TTCTCCTACC TGCGGCGTGT AGTGGAGAGG 
AGCTCTGGCC TGGAGTTTGC AGCCAAGTTC ATCCCTAGCC AGGCTAAGCC TAAAGCATCA 
GCAAGACGCG AGGCGCGCTT GCTGGCCCGG CTCCAGCATG GCTGTGTGCT CTACTTCCAC 
GAAGCCTTTG AGAGGCGCAG GGGACTTGTC ATAGTCACGG AGCTCTGCAC AGAAGAGCTT 
CTAGAGCGCA TGGCCAGGAA GCCCACCGTG TGTGAGTCTG AGACTCGGAC CTATATGCGT 
CAAGTGCTAG AGGGGATATG CTATCTGCAT CAGAGCCATG TGCTGCACCT GGATGTCAAG 
CCGGAGAACC TGCTTGTGTG GGATGGTGCA GGGGGTGAAG AGCAGGTGCG CATCTGTGAC 
TTCGGGAACG CCCAGGAACT CACTCCGGGA GAGCCCCAGT ACTGTCAGTA TGGCACGCCA 
GAGTTTGTAG CACCTGAGAT TGTCAACCAG AGCCCTGTGT CTGGAGTCAC TGACATCTGG 
CCCGTGGGCG TCGTTGCCTT TCTCTGTTTG ACGGGCATCT CCCCATTTGT TGGGGAGAAT 
GACCGGACAA CACTCATGAA TATCCGAAAC TACAACGTGG CATTTGAGGA GACTACGTTC 
CTGAGTCTGA GCAGGGAGGC CCGGGGCTTC CTCATCAAAG TGCTGGTGCA GGATCGACTG 
AGACCTACTG CAGAAGAGAC ATTGGAACAT CCTTGGTTCA AAACAGAAGC AAAGGGTGCA 
GAGGTGAGCA CAGATCACCT GAAGCTCTTC CTGTCACGGA GGAGGTGGCA GCGCTCTCAA 
ATCAGCTACA AGTGCCACCT GGTGCTGCGC CCCATTCCGG AACTGCTACG GGCTCCCCCG 
GAGCGGGTGT GGGTGGCCAT GCCTAGAAGA CAACCCCCGA GTGGGGGACT CTCATCCTCT 
TCAGACTCAG AAGAGGAAGA GTTGGAGGAA CTTCCCTCAG TACCCCGCCC CCTGCAGCCT 
GAGTTCTCAG GTTCTCGGGT ATCCCTCACC GACATCCCCA CCGAAGATGA GGCTCTGGGG 
ACCCCGGAAG CTGGGGCTGC CACCCCCATG GACTGGCAAG AGCAGGAAAG GACTCCCTCT 
AAGGACCAGG AGGCCCCCAG CCCTGAGGCA CTCCCCTCTC CAGGCCAGGA GTCCCCTGAT 
GGGCCTAGCC CCAGGCGGCC AGAGCTCCGC AGGGGTAGCT CTGCTGAGAG TGCCCTGCCC 
CGAGTCGGGT CGCGGGAACC GGGCCGGAGC TTGCACAAGG CAGCATCCGT GGAGCTGCCG 
CAGCGCAGGA GCCCCAGCCC GGGTGCCACC CGCCTGACTC GGGGAGGCCT GGGTGAGGGC 
GAGTATGCCC AGAGGCTGCA GGCCCTGCGT CAGCGGCTGC TGAGGGGAGG CCCCGAGGAT 
GGCAAGGTCA GCGGCCTCAG GGGACCCCTG CTGGAGAGCC TGGGGGGCCG TGCACGGGAT 
CCCAGGATGG CAAGGGCTGC CTCCAGCGAG GCTGCCCCCC ACCACCAGCC CCCTCCGGAG 
TCCCGGGGTC TGCAAAAGAG CAGCAGCTTC TCTCAGGGCG AGGCAGAGCC CCGGGGTCGG 
CACCGCCGCG CGGGGGCACC CCTCGAAATC CCCGTGGCCA GACTTGGGGC TCGCAGGCTG 
CAGGAGTCTC CCTCCTTATC TGCCCTTAGC GAGACCCAGC CACCGAGCCC TGCGAGGCCC 
AGCGTCCCCA AACTCAGTAT CACCAAGTCC CCGGAACCTT CCGCTGTCAC ATCCAGAGAC 
TCTCCCCAGC CCCCGGAACC TCAGCCTGTC CCAGAAAAGG TCCCTGAGCC CAAACCAGAA 
CCAGTCCGAG CTGCAAAACC AGCACAACCC CCTTTGGCTC TGCAAATGCC AACGCAGCCT 
CTCACCCCCT ATGCCCAGAT CATGCAGTCG CTCCAGCTGT CCAGTCCCAC CCTGTCCCCA 
CAGGACCCCG CTGTGCCTCC ATCAGAGCCC AAGCCCCACG CGGCTGTGTT TGCCAGGGTG 
GCTTCCCCAC CACCCGGAGT CTCTGAGAAG CGCGTGCCTT CGGCCAGAAC TCCCCCAGTG 
CTAGCCGAGA AAGCCCGAGT CCCCACGGTG CCCCCCAGGC CAGGCAGCAG CCTCAGCGGC 
AGCATCGAAA ACCTTGAATC AGAGGCTGTG TTCGAGGCTA AGTTCAAACG CAGCCGCGAA 
TCGCCACTGT CGCGGGGCCT GAGGCTACTG AGCCGCTCGC GCTCGGAGGA GCGCGGTCCC 
TTCCGTGGGG CGGAGGACGA CGGCATATAC CGGCCCAGTC CGGCAGGAAC GCCGCTAGAG 
CTGGTGCGAC GGCCCGAGCG CTCGCGCTCC GTGCAGGACC TCAGGGTAGC CGGAGAGCCG 
GGCCTTGTGC GTCGCCTTTC GCTGTCCCTG TCGCAGAAGC TGCGGCGGAC ACCGCCGGGA 
CAGCGACACC CTGCCTGGGA GTCCCGCAGC GGAGACGGGG AGAGCTCCGA GGGCGGCAGC 
TCAGCTCGGG CATCCCCAGT GCTGGCGGTA CGCAGGAGAC TCAGCTCCAC GCTGGAGCGT 
CTGTCCAGCC GTTTGCAGCG CAGCGGCAGC AGCGAGGACT CCGGGGGCGC CTCGGGTCGC 
AGCACGCCGC TGTTTGGACG GCTGCGCAGG GCTACTTCGG AAGGGGAGAG CCTGCGACGC 
CTCGGTGTTC CGCACAATCA GCTGGGCTCT CAGACTGGTG CCACTACACC TTCCGCTGAG 
TCTCTGGGCT CAGAGGCCAG TGGCACATCG GGTTCTTCAG CTCCTGGAGA AAGCCGAAGC 
CGGCACCGGT GGGGCCTTTC CAGGCTGCGC AAGGACAAGG GATTATCTCA GCCAAACCTC 
TCCTCCAGTG TCCAAGAGGA CTTGGGTCAC CAGTATGTGC CCAGTGAGTC AGATTTCCCC 
CCAGTCTTCC ACATCAAACT CAAGGACCAG GTGCTGTTGG AGGGGGAAGC AGCCACCCTG 
CTCTGCCTAC CCGCAGCCTG CCCAGCACCC CGCATCTCCT GGATGAAAGA TAAGCAGTCC 
CTGCGTTCAG AGCCCTCAGT GGTCATTGTG TCCTGCAAAG ATGGACGGCA GCTGCTGAGC 
ATACCCCGGG CCGGCAAGCG GCATGCTGGG CTCTATGAGT GCTCAGCCAC CAATGTCCTG 
GGCAGCATTA CCAGCTCCTG TACTGTGGCC GTGGCCCGCA TTCCAGGGAA GCTAGCTCCT 
CCAGAGGTGC CCCAGACCTA CCATGACACA GCACTAGTAG TGTGGAAGCC AGGAGACGGT 
CGGGCACCTT GCACATACAC TCTGGAGCGG CGGGTGGATG GGGAATCTGT CTGGCACCCT 
GTGAGCTCAG GCATCCCCGA CTGTTATTAC AATGTGACTC AGCTGCCTGT CGGCGTGACC 
GTGCGGTTCC GTGTGGCCTG TTCCAATCGT GCTGGGCAGG GGCCCTTCAG CAACCCTTCT 
GAGAAGGTCT TCATCCGAGG CACTCCAGAT TCTCCTGCTC AGCCAGCTGC TGCTCCCCGA 
GATGCCCCTG TTACCTCAGG GCCAACCAGG GCCCCACCGC CTGACTCTCC TACCTCACTG 
GCCCCCACCC CCGCTCTTGC GCCCCCAGCC TCCCAGGCAT CCACTCTCAG CCCCTCAACT 
TCCTCCATGT CTGCCAACCA GGCCTTGTCC TCACTCAAGG CTGTGGGTCC ACCACCCGCA 
ACCCCTCCAC GAAAGCACAG GGGCCTTCTG GCCACCCAGC AAGCAGAGCC ATCCCCACCC 
AGTATCGTGG TCACCCCAAG TGAACCCAGG TCTTTTGTCC CTGACACTGG AACCCTGACC 
CCAACCTCTA GTCCTCAAGG GGTTAAACCA GCACCTTCCT CAACTTCCTT GTATATGGTG 
ACTTCCTTCG TGTCTGCACC TCCAGCCCCC CAGGCCCCAG CCCCTGAGCC CCCTCCTGAG 
CCCACCAAGG TGACTGTGCG GAGCCTCAGC CCAGCCAAGG AGGTGGTCAG CTCCCCAACT 
CCTGAGAGCA CCACTCTTCG GCAGGGTCCC CCTCAGAAAC CCTACACCTT CCTGGAGGAG 
AAGGCCAGGG GCCGCTTTGG CGTTGTGCGG TCATGCCGGG AGAATGCTAC GGGCCGAACG 
TTTGTCGCCA AGATCGTGCC GTACGCCGCG GAGGGCAAGC GACGCGTGCT GCAGGAATAC 
GAGGTGCTTC GGACGCTGCA CCACGAGCGG CTCATGTCCC TGCACGAGGC CTACATCACC 
CCTCGGTACC TCGTGCTTAT CGCTGAGAGC TGTGGCAACC GGGAACTCCT CTGTGGGCTC 
AGCGACAGAT TCCGGTATTC AGAAGATGAC GTGGCCACCT ATGTGGTGCA GCTGCTACAA 
GGCCTGGACT ACCTCCACGG CCACCACGTG CTACACCTGG ACATCAAGCC GGACAATCTA 
CTGTTGGCCG CTGACAATGC CCTTAAAATT GTGGACTTCG GCAGTGCTCA GCCCTATAAC 
CCTCAGGCCC TGAAGCCACT TGGCCACCGC ACAGGCACTC TGGAGTTCAT GGCTCCTGAG 
ATGGTGAAGG GAGACCCCAT TGGCTCTGCC ACGGACATCT GGGGAGCGGG TGTGCTCACC 
TACATCATGC TTAGTGGGTA CTCCCCATTC TATGAGCCAG ACCCCCAGGA AACAGAGGCT 
CGGATTGTTG GGGGTCGCTT TGATGCCTTC CAGTTGTATC CTAACACATC CCAGAGTGCC 
ACCCTCTTCT TGAGAAAGGT CCTCTCAGTA CATCCCTGGA GCCGGCCCTC TCTGCAGGAC 
TGCTTGGCCC ACCCATGGCT GCAAGATGCC TACCTGATGA AGCTGCGCCG CCAGACACTC 
ACCTTCACCA CCAACCGGCT CAAGGAATTC CTGGGCGAGC AGCGGCGACG TCGGGCTGAG 
GCTGCTACCC GTCACAAGGT GCTGCTCCGC TCCTACCCTG GCAGCCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_011238646.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236948.1 Mus musculus SPEG complex locus (Speg), transcript variant X6, mRNA.

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CloneID	OMu67147
Clone ID Related Accession (Same CDS sequence)	XM_011238646.1 , XM_011238646.2
Accession Version	XM_011238646.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238646.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGACTC GGAGACTGCC GAGGATGACA TCAGCGATGT GCCGGGGACC 
CAGCGCCTGG AGCTTCGGGA TGACAGGGCC TTCAGCACCC CCACGGGGGG CTCTGATACC 
CTGGTGGGTA CCTCCCTGGA CACACCCCCG ACCTCCGTGA CAGGCACCTC AGAGGAGCAA 
GTGAGCTGGT GGGGCAGCGG GCAGACGGTC CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC 
ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA GCACAGACAA CCGGGGCCGG GCCACGGCAC 
CTGGGGGTGG AGCCGCTGGT GCGGGCATCG CGAGCTAATC TGGTGGGCGC AAGCTGGGGG 
TCAGAGGATA GCCTTTCGGT GGCCAGTGAC CTCTATGGCA GCGCGTTCAG TCTGTACAGA 
GGACGGGCGC TCTCGATCCA CGTCAGCATT CCTCCGAGTG GATTGCACAG AGAGGAGCCT 
GACCTTCAGC CTCAGCCAGC CAGTGATGCC CTTCGTCCAC GCCCTGCCCT TCCACCTCCC 
TCCAAATCCG CTTTGCTTCC CCCACCATCT CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA 
CCTAGTACCC AGCCCCCAGC TACTCCCACT TCGCCCCATC GGCGTGCTCA GGAGCCCTCG 
CTGCCTGAGG ACATCACCAC CACGGAAGAG AAGCGAGGGA AAAAGCCCAA GTCATCCGGG 
CCGTCGCTGG CGGGCACAGT GGAGTCCCGG CCCCAGACGC CACTGAGCGA GGCTTCGGGT 
CGCCTGTCAG CATTGGGCCG CTCGCCCCGG CTGGTGCGCG CGGGGTCCCG CATCCTGGAC 
AAGCTACAGT TCTTCGAAGA GCGGCGACGC AGCCTGGAGC GCAGCGACTC GCCGCCAGCG 
CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG 
GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC 
AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG CAAAAGGCGG CCTCGTTGGA TGAACGCACG 
CGACAACGCA GTGCAACCTC GGACCTCGAA CTCCGCTTCG CCCAGGAGCT GGGTCGCATC 
CGCCGATCTA CGTCGCGGGA GGAGCTGGTG CGTTCGCACG AGTCCCTGCG TGCCACGCTG 
CAGCGCGCCC CATCCCCTCG GGAGCCCGGC GAGCCCCCAC TCTTCTCCCG GCCTTCCACA 
CCCAAGACCT CACGGGCTGT GAGCCCGGCT GCCACCCAGC CGCCGCCTCC TAGTGGTGCG 
GGCAAATCTG GGGACGAGCC TGGGAGGCCC CGAAGCAGAG GGCCGGTGGG CAGGACTGAA 
CCGGGGGAAG GCCCGCAGCA GGAGATCAAG CGTCGGGACC AATTCCCGCT AACCAGGAGC 
AGAGCCATCC AGGAGTGCAG GAGCCCTGTG CCGCCCTACA CCGCGGATCC CCCGGAGAGC 
AGGACAAAAG CCCCCTCCGG TCGCAAGCGG GAACCCCCTG CTCAAGCGGT GCGCTTTCTG 
CCCTGGGCCA CTCCGGGAGT GGAGGACTCT GTTCTGCCCC AAACCTTGGA GAAGAATAGA 
GCGGGACCCG AGGCTGAGAA GAGGCTTCGC AGAGGACCTG AGGAGGATGG CCCCTGGGGG 
CCCTGGGACC GCAGAGGGAC CCGCAGCCAA GGCAAAGGTC GCCGTGCTCG GCCTACTTCC 
CCCGAGCTCG AGTCCTCAGA CGACTCCTAT GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA 
CCCGTGTTTG AGATCCCTCT GCAGAATATG GTGGTGGCGC CAGGAGCTGA CGTGCTACTT 
AAGTGTATCA TCACCGCCAA CCCCCCACCC CAAGTGTCCT GGAAAAAGGA TGGGTCCATG 
TTGCACAGCG AGGGTCGTCT TCTCATCCGG GCTGAAGGTG AACGGCACAC ACTGCTGCTC 
AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC TACACAGCCA CTGCCACCAA CGAACTGGGC 
CAAGCTACCT GTGCTTCTTC ACTGGCTGTG AGACCTGGCG GCTCCACATC CCCTTTCAGC 
AGCCCCATCA CCTCTGATGA GGAGTACCTG AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG 
GAGACCTGGC CCCGAACCCC TACCATGAAG CTCAGTCCCA GCCAGGATCA TGATTCCTCC 
GACTCTTCTT CCAAGGCACC CCCAACGTTC AAGGTCTCAC TCATGGACCA ATCGGTGAGA 
GAAGGTCAAG ATGTCATTAT GAGCATCCGT GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC 
TGGCTGAGGA ATCGACAGCC CGTGCGCCCA GACCAGCGGC GCTTTGCAGA GGAGGCCGAG 
GGTGGGCTCT GCCGCTTGAG GATCCTGGCT GCTGAACGGG GCGATGCTGG TTTCTACACA 
TGCAAGGCGG TCAACGAATA TGGCGCTCGG CAGTGCGAGG CCCGCCTGGA GGTCCGAGCA 
CACCCTGAAA GCCGGTCCCT GGCCGTGCTG GCCCCCCTGC AGGACGTGGA CGTGGGGGCC 
GGGGAGATGG CGCTGTTTGA GTGCCTGGTG GCAGGTCCAG CTGACGTCGA GGTGGACTGG 
CTGTGCCGGG GCCGCCTGCT GCAGCCTGCG CTGCTCAAAT GCAAGATGCA TTTTGATGGC 
CGCAAGTGCA AGCTGCTGCT CACCTCTGTG CACGAGGACG ACAGTGGTGT CTACACCTGC 
AAGCTGAGCA CAGCCAAAGA TGAACTGACC TGCAGTGCCC GGCTGACGGT TCGGCCGTCG 
CTGGCGCCTC TCTTCACCCG GTTGCTGGAA GATGTGGAGG TGTTGGAAGG CCGTGCTGCC 
CGCTTGGATT GCAAGATCAG CGGTACCCCG CCTCCCTCCG TTACCTGGAC TCATTTTGGC 
CACCCCGTGA ATGAGGGTGA CAACTTGCGG CTTCAACAGG ATGGAGGTCT ACACTCACTG 
CACATTGCCC GGGTGGGCAG TGAGGACGAA GGACTCTATG AAGTCAGTGC CACCAACACT 
CATGGCCAGG CCCACTGCTC CGCCCAGCTG TATGTGGAGG AGCCTCGGAC AGCTGCCTCT 
GGCCCCAGCT CGAAGCTGGA GAAGATGCCA TCTATCCCTG AGGAGCCGGA GCACGGAGAT 
CTAGAGAGGC TGTCTATTCC TGACTTCCTA CGGCCACTGC AAGATCTGGA AGTGGGACTA 
GCTAAGGAGG CCATGCTGGA GTGCCAAGTG ACCGGCCTGC CCTATCCCAC CATCAGCTGG 
TTCCACAATG GCCACCGCAT TCAAAGCAGT GACGACCGAC GCATGACACA GTACAGGGAC 
ATACATCGCC TGGTGTTCCC AGCTGTGGGG CCTCAGCATG CTGGTGTCTA TAAGAGTGTA 
ATCGCCAACA AGCTGGGCAA AGCGGCCTGC TACGCCCATC TCTATGTCAC AGATGTGGTT 
CCAGGCCCTC CAGATGGTGC TCCGGAAGTA GTAGCTGTGA CCGGAAGGAT GGTCACACTA 
TCGTGGAACC CACCCAGGAG TCTGGACATG GCTATTGACC CAGATTCCCT AACATACACT 
GTGCAGCACC AGGTGTTGGG CTCGGACCAG TGGACGGCAC TGGTCACAGG CCTGCGGGAG 
CCTGCGTGGG CAGCCACAGG GCTGAAGAAG GGGATCCAGC ACATCTTCCG GGTCCTCAGC 
TCCAGTGGCA AGAGCAGCAG CAAGCCCTCA GCACCGTCGG AACCCGTGCA GCTGCTAGAG 
CATGGCCCAC CCCTGGAAGA GGCGCCTGCT GTGCTGGACA AGCAGGACAT TGTGTATGTG 
GTAGAGGGCC AGCCTGCCTG TGTCACCGTC ACTTTTAACC ATGTGGAGGC CCAGGTTGTC 
TGGAGGAGCT GCCGGGGAGC CCTCCTCGAG GCGCGCACAG GTGTGTATGA ACTGAGTCAG 
CCAGATGACG ACCAGTATTG TCTGCGGATC TGCCGGGTGA GCCGACGAGA CCTGGGACCC 
CTTACTTGCA GTGCCCGAAA CCGCCACGGC ACAAAGGCCT GCTCGGTCAC CCTGGAGCTG 
GCAGAGGCAC CTCGGTTTGA GTCTATCATG GAAGATGTGG AGGTGGGGCC TGGAGAAACC 
GCTCGCTTTG CTGTGGTAGT TGAGGGGAAA CCACTGCCAG ACATCATGTG GTACAAGGAT 
GAGGTGCTGC TGGCCGAGAG CAACCATGTG AGCTTCGTGT ATGAGGAGAA CGAGTGTTCC 
CTAGTGCTGC TCAGCGCAGG AAGCCAGGAT GGAGGCGTCT ATACCTGTAC CGCCCGGAAC 
CTGGCAGGCG AAGTCTCCTG CAAGGCGGAG CTGTCTGTGC TTTCAGCTCA GACAGCCATG 
GAGGTCGAGG GGGTCGGAGA GGATGAGGAA CACCGAGGAA GAAGACTTAG CGACTACTAC 
GACATCCACC AAGAGATTGG CAGGGGTGCC TTCTCCTACC TGCGGCGTGT AGTGGAGAGG 
AGCTCTGGCC TGGAGTTTGC AGCCAAGTTC ATCCCTAGCC AGGCTAAGCC TAAAGCATCA 
GCAAGACGCG AGGCGCGCTT GCTGGCCCGG CTCCAGCATG GCTGTGTGCT CTACTTCCAC 
GAAGCCTTTG AGAGGCGCAG GGGACTTGTC ATAGTCACGG AGCTCTGCAC AGAAGAGCTT 
CTAGAGCGCA TGGCCAGGAA GCCCACCGTG TGTGAGTCTG AGACTCGGAC CTATATGCGT 
CAAGTGCTAG AGGGGATATG CTATCTGCAT CAGAGCCATG TGCTGCACCT GGATGTCAAG 
CCGGAGAACC TGCTTGTGTG GGATGGTGCA GGGGGTGAAG AGCAGGTGCG CATCTGTGAC 
TTCGGGAACG CCCAGGAACT CACTCCGGGA GAGCCCCAGT ACTGTCAGTA TGGCACGCCA 
GAGTTTGTAG CACCTGAGAT TGTCAACCAG AGCCCTGTGT CTGGAGTCAC TGACATCTGG 
CCCGTGGGCG TCGTTGCCTT TCTCTGTTTG ACGGGCATCT CCCCATTTGT TGGGGAGAAT 
GACCGGACAA CACTCATGAA TATCCGAAAC TACAACGTGG CATTTGAGGA GACTACGTTC 
CTGAGTCTGA GCAGGGAGGC CCGGGGCTTC CTCATCAAAG TGCTGGTGCA GGATCGACTG 
AGACCTACTG CAGAAGAGAC ATTGGAACAT CCTTGGTTCA AAACAGAAGC AAAGGGTGCA 
GAGGTGAGCA CAGATCACCT GAAGCTCTTC CTGTCACGGA GGAGGTGGCA GCGCTCTCAA 
ATCAGCTACA AGTGCCACCT GGTGCTGCGC CCCATTCCGG AACTGCTACG GGCTCCCCCG 
GAGCGGGTGT GGGTGGCCAT GCCTAGAAGA CAACCCCCGA GTGGGGGACT CTCATCCTCT 
TCAGACTCAG AAGAGGAAGA GTTGGAGGAA CTTCCCTCAG TACCCCGCCC CCTGCAGCCT 
GAGTTCTCAG GTTCTCGGGT ATCCCTCACC GACATCCCCA CCGAAGATGA GGCTCTGGGG 
ACCCCGGAAG CTGGGGCTGC CACCCCCATG GACTGGCAAG AGCAGGAAAG GACTCCCTCT 
AAGGACCAGG AGGCCCCCAG CCCTGAGGCA CTCCCCTCTC CAGGCCAGGA GTCCCCTGAT 
GGGCCTAGCC CCAGGCGGCC AGAGCTCCGC AGGGGTAGCT CTGCTGAGAG TGCCCTGCCC 
CGAGTCGGGT CGCGGGAACC GGGCCGGAGC TTGCACAAGG CAGCATCCGT GGAGCTGCCG 
CAGCGCAGGA GCCCCAGCCC GGGTGCCACC CGCCTGACTC GGGGAGGCCT GGGTGAGGGC 
GAGTATGCCC AGAGGCTGCA GGCCCTGCGT CAGCGGCTGC TGAGGGGAGG CCCCGAGGAT 
GGCAAGGTCA GCGGCCTCAG GGGACCCCTG CTGGAGAGCC TGGGGGGCCG TGCACGGGAT 
CCCAGGATGG CAAGGGCTGC CTCCAGCGAG GCTGCCCCCC ACCACCAGCC CCCTCCGGAG 
TCCCGGGGTC TGCAAAAGAG CAGCAGCTTC TCTCAGGGCG AGGCAGAGCC CCGGGGTCGG 
CACCGCCGCG CGGGGGCACC CCTCGAAATC CCCGTGGCCA GACTTGGGGC TCGCAGGCTG 
CAGGAGTCTC CCTCCTTATC TGCCCTTAGC GAGACCCAGC CACCGAGCCC TGCGAGGCCC 
AGCGTCCCCA AACTCAGTAT CACCAAGTCC CCGGAACCTT CCGCTGTCAC ATCCAGAGAC 
TCTCCCCAGC CCCCGGAACC TCAGCCTGTC CCAGAAAAGG TCCCTGAGCC CAAACCAGAA 
CCAGTCCGAG CTGCAAAACC AGCACAACCC CCTTTGGCTC TGCAAATGCC AACGCAGCCT 
CTCACCCCCT ATGCCCAGAT CATGCAGTCG CTCCAGCTGT CCAGTCCCAC CCTGTCCCCA 
CAGGACCCCG CTGTGCCTCC ATCAGAGCCC AAGCCCCACG CGGCTGTGTT TGCCAGGGTG 
GCTTCCCCAC CACCCGGAGT CTCTGAGAAG CGCGTGCCTT CGGCCAGAAC TCCCCCAGTG 
CTAGCCGAGA AAGCCCGAGT CCCCACGGTG CCCCCCAGGC CAGGCAGCAG CCTCAGCGGC 
AGCATCGAAA ACCTTGAATC AGAGGCTGTG TTCGAGGCTA AGTTCAAACG CAGCCGCGAA 
TCGCCACTGT CGCGGGGCCT GAGGCTACTG AGCCGCTCGC GCTCGGAGGA GCGCGGTCCC 
TTCCGTGGGG CGGAGGACGA CGGCATATAC CGGCCCAGTC CGGCAGGAAC GCCGCTAGAG 
CTGGTGCGAC GGCCCGAGCG CTCGCGCTCC GTGCAGGACC TCAGGGTAGC CGGAGAGCCG 
GGCCTTGTGC GTCGCCTTTC GCTGTCCCTG TCGCAGAAGC TGCGGCGGAC ACCGCCGGGA 
CAGCGACACC CTGCCTGGGA GTCCCGCAGC GGAGACGGGG AGAGCTCCGA GGGCGGCAGC 
TCAGCTCGGG CATCCCCAGT GCTGGCGGTA CGCAGGAGAC TCAGCTCCAC GCTGGAGCGT 
CTGTCCAGCC GTTTGCAGCG CAGCGGCAGC AGCGAGGACT CCGGGGGCGC CTCGGGTCGC 
AGCACGCCGC TGTTTGGACG GCTGCGCAGG GCTACTTCGG AAGGGGAGAG CCTGCGACGC 
CTCGGTGTTC CGCACAATCA GCTGGGCTCT CAGACTGGTG CCACTACACC TTCCGCTGAG 
TCTCTGGGCT CAGAGGCCAG TGGCACATCG GGTTCTTCAG CTCCTGGAGA AAGCCGAAGC 
CGGCACCGGT GGGGCCTTTC CAGGCTGCGC AAGGACAAGG GATTATCTCA GCCAAACCTC 
TCCTCCAGTG TCCAAGAGGA CTTGGGTCAC CAGTATGTGC CCAGTGAGTC AGATTTCCCC 
CCAGTCTTCC ACATCAAACT CAAGGACCAG GTGCTGTTGG AGGGGGAAGC AGCCACCCTG 
CTCTGCCTAC CCGCAGCCTG CCCAGCACCC CGCATCTCCT GGATGAAAGA TAAGCAGTCC 
CTGCGTTCAG AGCCCTCAGT GGTCATTGTG TCCTGCAAAG ATGGACGGCA GCTGCTGAGC 
ATACCCCGGG CCGGCAAGCG GCATGCTGGG CTCTATGAGT GCTCAGCCAC CAATGTCCTG 
GGCAGCATTA CCAGCTCCTG TACTGTGGCC GTGGCCCGCA TTCCAGGGAA GCTAGCTCCT 
CCAGAGGTGC CCCAGACCTA CCATGACACA GCACTAGTAG TGTGGAAGCC AGGAGACGGT 
CGGGCACCTT GCACATACAC TCTGGAGCGG CGGGTGGATG GGGAATCTGT CTGGCACCCT 
GTGAGCTCAG GCATCCCCGA CTGTTATTAC AATGTGACTC AGCTGCCTGT CGGCGTGACC 
GTGCGGTTCC GTGTGGCCTG TTCCAATCGT GCTGGGCAGG GGCCCTTCAG CAACCCTTCT 
GAGAAGGTCT TCATCCGAGG CACTCCAGAT TCTCCTGCTC AGCCAGCTGC TGCTCCCCGA 
GATGCCCCTG TTACCTCAGG GCCAACCAGG GCCCCACCGC CTGACTCTCC TACCTCACTG 
GCCCCCACCC CCGCTCTTGC GCCCCCAGCC TCCCAGGCAT CCACTCTCAG CCCCTCAACT 
TCCTCCATGT CTGCCAACCA GGCCTTGTCC TCACTCAAGG CTGTGGGTCC ACCACCCGCA 
ACCCCTCCAC GAAAGCACAG GGGCCTTCTG GCCACCCAGC AAGCAGAGCC ATCCCCACCC 
AGTATCGTGG TCACCCCAAG TGAACCCAGG TCTTTTGTCC CTGACACTGG AACCCTGACC 
CCAACCTCTA GTCCTCAAGG GGTTAAACCA GCACCTTCCT CAACTTCCTT GTATATGGTG 
ACTTCCTTCG TGTCTGCACC TCCAGCCCCC CAGGCCCCAG CCCCTGAGCC CCCTCCTGAG 
CCCACCAAGG TGACTGTGCG GAGCCTCAGC CCAGCCAAGG AGGTGGTCAG CTCCCCAACT 
CCTGAGAGCA CCACTCTTCG GCAGGGTCCC CCTCAGAAAC CCTACACCTT CCTGGAGGAG 
AAGGCCAGGG GCCGCTTTGG CGTTGTGCGG TCATGCCGGG AGAATGCTAC GGGCCGAACG 
TTTGTCGCCA AGATCGTGCC GTACGCCGCG GAGGGCAAGC GACGCGTGCT GCAGGAATAC 
GAGGTGCTTC GGACGCTGCA CCACGAGCGG CTCATGTCCC TGCACGAGGC CTACATCACC 
CCTCGGTACC TCGTGCTTAT CGCTGAGAGC TGTGGCAACC GGGAACTCCT CTGTGGGCTC 
AGCGACAGAT TCCGGTATTC AGAAGATGAC GTGGCCACCT ATGTGGTGCA GCTGCTACAA 
GGCCTGGACT ACCTCCACGG CCACCACGTG CTACACCTGG ACATCAAGCC GGACAATCTA 
CTGTTGGCCG CTGACAATGC CCTTAAAATT GTGGACTTCG GCAGTGCTCA GCCCTATAAC 
CCTCAGGCCC TGAAGCCACT TGGCCACCGC ACAGGCACTC TGGAGTTCAT GGCTCCTGAG 
ATGGTGAAGG GAGACCCCAT TGGCTCTGCC ACGGACATCT GGGGAGCGGG TGTGCTCACC 
TACATCATGC TTAGTGGGTA CTCCCCATTC TATGAGCCAG ACCCCCAGGA AACAGAGGCT 
CGGATTGTTG GGGGTCGCTT TGATGCCTTC CAGTTGTATC CTAACACATC CCAGAGTGCC 
ACCCTCTTCT TGAGAAAGGT CCTCTCAGTA CATCCCTGGA GCCGGCCCTC TCTGCAGGAC 
TGCTTGGCCC ACCCATGGCT GCAAGATGCC TACCTGATGA AGCTGCGCCG CCAGACACTC 
ACCTTCACCA CCAACCGGCT CAAGGAATTC CTGGGCGAGC AGCGGCGACG TCGGGCTGAG 
GCTGCTACCC GTCACAAGGT GCTGCTCCGC TCCTACCCTG GCAGCCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236948.1
CDS	1364..10834
Translation	MKKLWVKKRFQKTGHSRRAFGRLTHDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPRQAQTTGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_011238646.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238646.2

ORF Insert Sequence:

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGACTC GGAGACTGCC GAGGATGACA TCAGCGATGT GCCGGGGACC 
CAGCGCCTGG AGCTTCGGGA TGACAGGGCC TTCAGCACCC CCACGGGGGG CTCTGATACC 
CTGGTGGGTA CCTCCCTGGA CACACCCCCG ACCTCCGTGA CAGGCACCTC AGAGGAGCAA 
GTGAGCTGGT GGGGCAGCGG GCAGACGGTC CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC 
ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA GCACAGACAA CCGGGGCCGG GCCACGGCAC 
CTGGGGGTGG AGCCGCTGGT GCGGGCATCG CGAGCTAATC TGGTGGGCGC AAGCTGGGGG 
TCAGAGGATA GCCTTTCGGT GGCCAGTGAC CTCTATGGCA GCGCGTTCAG TCTGTACAGA 
GGACGGGCGC TCTCGATCCA CGTCAGCATT CCTCCGAGTG GATTGCACAG AGAGGAGCCT 
GACCTTCAGC CTCAGCCAGC CAGTGATGCC CTTCGTCCAC GCCCTGCCCT TCCACCTCCC 
TCCAAATCCG CTTTGCTTCC CCCACCATCT CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA 
CCTAGTACCC AGCCCCCAGC TACTCCCACT TCGCCCCATC GGCGTGCTCA GGAGCCCTCG 
CTGCCTGAGG ACATCACCAC CACGGAAGAG AAGCGAGGGA AAAAGCCCAA GTCATCCGGG 
CCGTCGCTGG CGGGCACAGT GGAGTCCCGG CCCCAGACGC CACTGAGCGA GGCTTCGGGT 
CGCCTGTCAG CATTGGGCCG CTCGCCCCGG CTGGTGCGCG CGGGGTCCCG CATCCTGGAC 
AAGCTACAGT TCTTCGAAGA GCGGCGACGC AGCCTGGAGC GCAGCGACTC GCCGCCAGCG 
CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG 
GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC 
AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG CAAAAGGCGG CCTCGTTGGA TGAACGCACG 
CGACAACGCA GTGCAACCTC GGACCTCGAA CTCCGCTTCG CCCAGGAGCT GGGTCGCATC 
CGCCGATCTA CGTCGCGGGA GGAGCTGGTG CGTTCGCACG AGTCCCTGCG TGCCACGCTG 
CAGCGCGCCC CATCCCCTCG GGAGCCCGGC GAGCCCCCAC TCTTCTCCCG GCCTTCCACA 
CCCAAGACCT CACGGGCTGT GAGCCCGGCT GCCACCCAGC CGCCGCCTCC TAGTGGTGCG 
GGCAAATCTG GGGACGAGCC TGGGAGGCCC CGAAGCAGAG GGCCGGTGGG CAGGACTGAA 
CCGGGGGAAG GCCCGCAGCA GGAGATCAAG CGTCGGGACC AATTCCCGCT AACCAGGAGC 
AGAGCCATCC AGGAGTGCAG GAGCCCTGTG CCGCCCTACA CCGCGGATCC CCCGGAGAGC 
AGGACAAAAG CCCCCTCCGG TCGCAAGCGG GAACCCCCTG CTCAAGCGGT GCGCTTTCTG 
CCCTGGGCCA CTCCGGGAGT GGAGGACTCT GTTCTGCCCC AAACCTTGGA GAAGAATAGA 
GCGGGACCCG AGGCTGAGAA GAGGCTTCGC AGAGGACCTG AGGAGGATGG CCCCTGGGGG 
CCCTGGGACC GCAGAGGGAC CCGCAGCCAA GGCAAAGGTC GCCGTGCTCG GCCTACTTCC 
CCCGAGCTCG AGTCCTCAGA CGACTCCTAT GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA 
CCCGTGTTTG AGATCCCTCT GCAGAATATG GTGGTGGCGC CAGGAGCTGA CGTGCTACTT 
AAGTGTATCA TCACCGCCAA CCCCCCACCC CAAGTGTCCT GGAAAAAGGA TGGGTCCATG 
TTGCACAGCG AGGGTCGTCT TCTCATCCGG GCTGAAGGTG AACGGCACAC ACTGCTGCTC 
AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC TACACAGCCA CTGCCACCAA CGAACTGGGC 
CAAGCTACCT GTGCTTCTTC ACTGGCTGTG AGACCTGGCG GCTCCACATC CCCTTTCAGC 
AGCCCCATCA CCTCTGATGA GGAGTACCTG AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG 
GAGACCTGGC CCCGAACCCC TACCATGAAG CTCAGTCCCA GCCAGGATCA TGATTCCTCC 
GACTCTTCTT CCAAGGCACC CCCAACGTTC AAGGTCTCAC TCATGGACCA ATCGGTGAGA 
GAAGGTCAAG ATGTCATTAT GAGCATCCGT GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC 
TGGCTGAGGA ATCGACAGCC CGTGCGCCCA GACCAGCGGC GCTTTGCAGA GGAGGCCGAG 
GGTGGGCTCT GCCGCTTGAG GATCCTGGCT GCTGAACGGG GCGATGCTGG TTTCTACACA 
TGCAAGGCGG TCAACGAATA TGGCGCTCGG CAGTGCGAGG CCCGCCTGGA GGTCCGAGCA 
CACCCTGAAA GCCGGTCCCT GGCCGTGCTG GCCCCCCTGC AGGACGTGGA CGTGGGGGCC 
GGGGAGATGG CGCTGTTTGA GTGCCTGGTG GCAGGTCCAG CTGACGTCGA GGTGGACTGG 
CTGTGCCGGG GCCGCCTGCT GCAGCCTGCG CTGCTCAAAT GCAAGATGCA TTTTGATGGC 
CGCAAGTGCA AGCTGCTGCT CACCTCTGTG CACGAGGACG ACAGTGGTGT CTACACCTGC 
AAGCTGAGCA CAGCCAAAGA TGAACTGACC TGCAGTGCCC GGCTGACGGT TCGGCCGTCG 
CTGGCGCCTC TCTTCACCCG GTTGCTGGAA GATGTGGAGG TGTTGGAAGG CCGTGCTGCC 
CGCTTGGATT GCAAGATCAG CGGTACCCCG CCTCCCTCCG TTACCTGGAC TCATTTTGGC 
CACCCCGTGA ATGAGGGTGA CAACTTGCGG CTTCAACAGG ATGGAGGTCT ACACTCACTG 
CACATTGCCC GGGTGGGCAG TGAGGACGAA GGACTCTATG AAGTCAGTGC CACCAACACT 
CATGGCCAGG CCCACTGCTC CGCCCAGCTG TATGTGGAGG AGCCTCGGAC AGCTGCCTCT 
GGCCCCAGCT CGAAGCTGGA GAAGATGCCA TCTATCCCTG AGGAGCCGGA GCACGGAGAT 
CTAGAGAGGC TGTCTATTCC TGACTTCCTA CGGCCACTGC AAGATCTGGA AGTGGGACTA 
GCTAAGGAGG CCATGCTGGA GTGCCAAGTG ACCGGCCTGC CCTATCCCAC CATCAGCTGG 
TTCCACAATG GCCACCGCAT TCAAAGCAGT GACGACCGAC GCATGACACA GTACAGGGAC 
ATACATCGCC TGGTGTTCCC AGCTGTGGGG CCTCAGCATG CTGGTGTCTA TAAGAGTGTA 
ATCGCCAACA AGCTGGGCAA AGCGGCCTGC TACGCCCATC TCTATGTCAC AGATGTGGTT 
CCAGGCCCTC CAGATGGTGC TCCGGAAGTA GTAGCTGTGA CCGGAAGGAT GGTCACACTA 
TCGTGGAACC CACCCAGGAG TCTGGACATG GCTATTGACC CAGATTCCCT AACATACACT 
GTGCAGCACC AGGTGTTGGG CTCGGACCAG TGGACGGCAC TGGTCACAGG CCTGCGGGAG 
CCTGCGTGGG CAGCCACAGG GCTGAAGAAG GGGATCCAGC ACATCTTCCG GGTCCTCAGC 
TCCAGTGGCA AGAGCAGCAG CAAGCCCTCA GCACCGTCGG AACCCGTGCA GCTGCTAGAG 
CATGGCCCAC CCCTGGAAGA GGCGCCTGCT GTGCTGGACA AGCAGGACAT TGTGTATGTG 
GTAGAGGGCC AGCCTGCCTG TGTCACCGTC ACTTTTAACC ATGTGGAGGC CCAGGTTGTC 
TGGAGGAGCT GCCGGGGAGC CCTCCTCGAG GCGCGCACAG GTGTGTATGA ACTGAGTCAG 
CCAGATGACG ACCAGTATTG TCTGCGGATC TGCCGGGTGA GCCGACGAGA CCTGGGACCC 
CTTACTTGCA GTGCCCGAAA CCGCCACGGC ACAAAGGCCT GCTCGGTCAC CCTGGAGCTG 
GCAGAGGCAC CTCGGTTTGA GTCTATCATG GAAGATGTGG AGGTGGGGCC TGGAGAAACC 
GCTCGCTTTG CTGTGGTAGT TGAGGGGAAA CCACTGCCAG ACATCATGTG GTACAAGGAT 
GAGGTGCTGC TGGCCGAGAG CAACCATGTG AGCTTCGTGT ATGAGGAGAA CGAGTGTTCC 
CTAGTGCTGC TCAGCGCAGG AAGCCAGGAT GGAGGCGTCT ATACCTGTAC CGCCCGGAAC 
CTGGCAGGCG AAGTCTCCTG CAAGGCGGAG CTGTCTGTGC TTTCAGCTCA GACAGCCATG 
GAGGTCGAGG GGGTCGGAGA GGATGAGGAA CACCGAGGAA GAAGACTTAG CGACTACTAC 
GACATCCACC AAGAGATTGG CAGGGGTGCC TTCTCCTACC TGCGGCGTGT AGTGGAGAGG 
AGCTCTGGCC TGGAGTTTGC AGCCAAGTTC ATCCCTAGCC AGGCTAAGCC TAAAGCATCA 
GCAAGACGCG AGGCGCGCTT GCTGGCCCGG CTCCAGCATG GCTGTGTGCT CTACTTCCAC 
GAAGCCTTTG AGAGGCGCAG GGGACTTGTC ATAGTCACGG AGCTCTGCAC AGAAGAGCTT 
CTAGAGCGCA TGGCCAGGAA GCCCACCGTG TGTGAGTCTG AGACTCGGAC CTATATGCGT 
CAAGTGCTAG AGGGGATATG CTATCTGCAT CAGAGCCATG TGCTGCACCT GGATGTCAAG 
CCGGAGAACC TGCTTGTGTG GGATGGTGCA GGGGGTGAAG AGCAGGTGCG CATCTGTGAC 
TTCGGGAACG CCCAGGAACT CACTCCGGGA GAGCCCCAGT ACTGTCAGTA TGGCACGCCA 
GAGTTTGTAG CACCTGAGAT TGTCAACCAG AGCCCTGTGT CTGGAGTCAC TGACATCTGG 
CCCGTGGGCG TCGTTGCCTT TCTCTGTTTG ACGGGCATCT CCCCATTTGT TGGGGAGAAT 
GACCGGACAA CACTCATGAA TATCCGAAAC TACAACGTGG CATTTGAGGA GACTACGTTC 
CTGAGTCTGA GCAGGGAGGC CCGGGGCTTC CTCATCAAAG TGCTGGTGCA GGATCGACTG 
AGACCTACTG CAGAAGAGAC ATTGGAACAT CCTTGGTTCA AAACAGAAGC AAAGGGTGCA 
GAGGTGAGCA CAGATCACCT GAAGCTCTTC CTGTCACGGA GGAGGTGGCA GCGCTCTCAA 
ATCAGCTACA AGTGCCACCT GGTGCTGCGC CCCATTCCGG AACTGCTACG GGCTCCCCCG 
GAGCGGGTGT GGGTGGCCAT GCCTAGAAGA CAACCCCCGA GTGGGGGACT CTCATCCTCT 
TCAGACTCAG AAGAGGAAGA GTTGGAGGAA CTTCCCTCAG TACCCCGCCC CCTGCAGCCT 
GAGTTCTCAG GTTCTCGGGT ATCCCTCACC GACATCCCCA CCGAAGATGA GGCTCTGGGG 
ACCCCGGAAG CTGGGGCTGC CACCCCCATG GACTGGCAAG AGCAGGAAAG GACTCCCTCT 
AAGGACCAGG AGGCCCCCAG CCCTGAGGCA CTCCCCTCTC CAGGCCAGGA GTCCCCTGAT 
GGGCCTAGCC CCAGGCGGCC AGAGCTCCGC AGGGGTAGCT CTGCTGAGAG TGCCCTGCCC 
CGAGTCGGGT CGCGGGAACC GGGCCGGAGC TTGCACAAGG CAGCATCCGT GGAGCTGCCG 
CAGCGCAGGA GCCCCAGCCC GGGTGCCACC CGCCTGACTC GGGGAGGCCT GGGTGAGGGC 
GAGTATGCCC AGAGGCTGCA GGCCCTGCGT CAGCGGCTGC TGAGGGGAGG CCCCGAGGAT 
GGCAAGGTCA GCGGCCTCAG GGGACCCCTG CTGGAGAGCC TGGGGGGCCG TGCACGGGAT 
CCCAGGATGG CAAGGGCTGC CTCCAGCGAG GCTGCCCCCC ACCACCAGCC CCCTCCGGAG 
TCCCGGGGTC TGCAAAAGAG CAGCAGCTTC TCTCAGGGCG AGGCAGAGCC CCGGGGTCGG 
CACCGCCGCG CGGGGGCACC CCTCGAAATC CCCGTGGCCA GACTTGGGGC TCGCAGGCTG 
CAGGAGTCTC CCTCCTTATC TGCCCTTAGC GAGACCCAGC CACCGAGCCC TGCGAGGCCC 
AGCGTCCCCA AACTCAGTAT CACCAAGTCC CCGGAACCTT CCGCTGTCAC ATCCAGAGAC 
TCTCCCCAGC CCCCGGAACC TCAGCCTGTC CCAGAAAAGG TCCCTGAGCC CAAACCAGAA 
CCAGTCCGAG CTGCAAAACC AGCACAACCC CCTTTGGCTC TGCAAATGCC AACGCAGCCT 
CTCACCCCCT ATGCCCAGAT CATGCAGTCG CTCCAGCTGT CCAGTCCCAC CCTGTCCCCA 
CAGGACCCCG CTGTGCCTCC ATCAGAGCCC AAGCCCCACG CGGCTGTGTT TGCCAGGGTG 
GCTTCCCCAC CACCCGGAGT CTCTGAGAAG CGCGTGCCTT CGGCCAGAAC TCCCCCAGTG 
CTAGCCGAGA AAGCCCGAGT CCCCACGGTG CCCCCCAGGC CAGGCAGCAG CCTCAGCGGC 
AGCATCGAAA ACCTTGAATC AGAGGCTGTG TTCGAGGCTA AGTTCAAACG CAGCCGCGAA 
TCGCCACTGT CGCGGGGCCT GAGGCTACTG AGCCGCTCGC GCTCGGAGGA GCGCGGTCCC 
TTCCGTGGGG CGGAGGACGA CGGCATATAC CGGCCCAGTC CGGCAGGAAC GCCGCTAGAG 
CTGGTGCGAC GGCCCGAGCG CTCGCGCTCC GTGCAGGACC TCAGGGTAGC CGGAGAGCCG 
GGCCTTGTGC GTCGCCTTTC GCTGTCCCTG TCGCAGAAGC TGCGGCGGAC ACCGCCGGGA 
CAGCGACACC CTGCCTGGGA GTCCCGCAGC GGAGACGGGG AGAGCTCCGA GGGCGGCAGC 
TCAGCTCGGG CATCCCCAGT GCTGGCGGTA CGCAGGAGAC TCAGCTCCAC GCTGGAGCGT 
CTGTCCAGCC GTTTGCAGCG CAGCGGCAGC AGCGAGGACT CCGGGGGCGC CTCGGGTCGC 
AGCACGCCGC TGTTTGGACG GCTGCGCAGG GCTACTTCGG AAGGGGAGAG CCTGCGACGC 
CTCGGTGTTC CGCACAATCA GCTGGGCTCT CAGACTGGTG CCACTACACC TTCCGCTGAG 
TCTCTGGGCT CAGAGGCCAG TGGCACATCG GGTTCTTCAG CTCCTGGAGA AAGCCGAAGC 
CGGCACCGGT GGGGCCTTTC CAGGCTGCGC AAGGACAAGG GATTATCTCA GCCAAACCTC 
TCCTCCAGTG TCCAAGAGGA CTTGGGTCAC CAGTATGTGC CCAGTGAGTC AGATTTCCCC 
CCAGTCTTCC ACATCAAACT CAAGGACCAG GTGCTGTTGG AGGGGGAAGC AGCCACCCTG 
CTCTGCCTAC CCGCAGCCTG CCCAGCACCC CGCATCTCCT GGATGAAAGA TAAGCAGTCC 
CTGCGTTCAG AGCCCTCAGT GGTCATTGTG TCCTGCAAAG ATGGACGGCA GCTGCTGAGC 
ATACCCCGGG CCGGCAAGCG GCATGCTGGG CTCTATGAGT GCTCAGCCAC CAATGTCCTG 
GGCAGCATTA CCAGCTCCTG TACTGTGGCC GTGGCCCGCA TTCCAGGGAA GCTAGCTCCT 
CCAGAGGTGC CCCAGACCTA CCATGACACA GCACTAGTAG TGTGGAAGCC AGGAGACGGT 
CGGGCACCTT GCACATACAC TCTGGAGCGG CGGGTGGATG GGGAATCTGT CTGGCACCCT 
GTGAGCTCAG GCATCCCCGA CTGTTATTAC AATGTGACTC AGCTGCCTGT CGGCGTGACC 
GTGCGGTTCC GTGTGGCCTG TTCCAATCGT GCTGGGCAGG GGCCCTTCAG CAACCCTTCT 
GAGAAGGTCT TCATCCGAGG CACTCCAGAT TCTCCTGCTC AGCCAGCTGC TGCTCCCCGA 
GATGCCCCTG TTACCTCAGG GCCAACCAGG GCCCCACCGC CTGACTCTCC TACCTCACTG 
GCCCCCACCC CCGCTCTTGC GCCCCCAGCC TCCCAGGCAT CCACTCTCAG CCCCTCAACT 
TCCTCCATGT CTGCCAACCA GGCCTTGTCC TCACTCAAGG CTGTGGGTCC ACCACCCGCA 
ACCCCTCCAC GAAAGCACAG GGGCCTTCTG GCCACCCAGC AAGCAGAGCC ATCCCCACCC 
AGTATCGTGG TCACCCCAAG TGAACCCAGG TCTTTTGTCC CTGACACTGG AACCCTGACC 
CCAACCTCTA GTCCTCAAGG GGTTAAACCA GCACCTTCCT CAACTTCCTT GTATATGGTG 
ACTTCCTTCG TGTCTGCACC TCCAGCCCCC CAGGCCCCAG CCCCTGAGCC CCCTCCTGAG 
CCCACCAAGG TGACTGTGCG GAGCCTCAGC CCAGCCAAGG AGGTGGTCAG CTCCCCAACT 
CCTGAGAGCA CCACTCTTCG GCAGGGTCCC CCTCAGAAAC CCTACACCTT CCTGGAGGAG 
AAGGCCAGGG GCCGCTTTGG CGTTGTGCGG TCATGCCGGG AGAATGCTAC GGGCCGAACG 
TTTGTCGCCA AGATCGTGCC GTACGCCGCG GAGGGCAAGC GACGCGTGCT GCAGGAATAC 
GAGGTGCTTC GGACGCTGCA CCACGAGCGG CTCATGTCCC TGCACGAGGC CTACATCACC 
CCTCGGTACC TCGTGCTTAT CGCTGAGAGC TGTGGCAACC GGGAACTCCT CTGTGGGCTC 
AGCGACAGAT TCCGGTATTC AGAAGATGAC GTGGCCACCT ATGTGGTGCA GCTGCTACAA 
GGCCTGGACT ACCTCCACGG CCACCACGTG CTACACCTGG ACATCAAGCC GGACAATCTA 
CTGTTGGCCG CTGACAATGC CCTTAAAATT GTGGACTTCG GCAGTGCTCA GCCCTATAAC 
CCTCAGGCCC TGAAGCCACT TGGCCACCGC ACAGGCACTC TGGAGTTCAT GGCTCCTGAG 
ATGGTGAAGG GAGACCCCAT TGGCTCTGCC ACGGACATCT GGGGAGCGGG TGTGCTCACC 
TACATCATGC TTAGTGGGTA CTCCCCATTC TATGAGCCAG ACCCCCAGGA AACAGAGGCT 
CGGATTGTTG GGGGTCGCTT TGATGCCTTC CAGTTGTATC CTAACACATC CCAGAGTGCC 
ACCCTCTTCT TGAGAAAGGT CCTCTCAGTA CATCCCTGGA GCCGGCCCTC TCTGCAGGAC 
TGCTTGGCCC ACCCATGGCT GCAAGATGCC TACCTGATGA AGCTGCGCCG CCAGACACTC 
ACCTTCACCA CCAACCGGCT CAAGGAATTC CTGGGCGAGC AGCGGCGACG TCGGGCTGAG 
GCTGCTACCC GTCACAAGGT GCTGCTCCGC TCCTACCCTG GCAGCCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_011238647.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236949.1 Mus musculus SPEG complex locus (Speg), transcript variant X8, mRNA.

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CloneID	OMu67148
Clone ID Related Accession (Same CDS sequence)	XM_011238647.2 , XM_011238647.1
Accession Version	XM_011238647.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238647.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGCAGC 
ATTCCTCCGA GTGGATTGCA CAGAGAGGAG CCTGACCTTC AGCCTCAGCC AGCCAGTGAT 
GCCCTTCGTC CACGCCCTGC CCTTCCACCT CCCTCCAAAT CCGCTTTGCT TCCCCCACCA 
TCTCCTCGGG TGGGAAAGAG GGCTCTGCCA GGACCTAGTA CCCAGCCCCC AGCTACTCCC 
ACTTCGCCCC ATCGGCGTGC TCAGGAGCCC TCGCTGCCTG AGGACATCAC CACCACGGAA 
GAGAAGCGAG GGAAAAAGCC CAAGTCATCC GGGCCGTCGC TGGCGGGCAC AGTGGAGTCC 
CGGCCCCAGA CGCCACTGAG CGAGGCTTCG GGTCGCCTGT CAGCATTGGG CCGCTCGCCC 
CGGCTGGTGC GCGCGGGGTC CCGCATCCTG GACAAGCTAC AGTTCTTCGA AGAGCGGCGA 
CGCAGCCTGG AGCGCAGCGA CTCGCCGCCA GCGCCCCTGC GGCCCTGGGT GCCCCTGCGC 
AAGGCTCGCT CGCTGGAGCA GCCGAAGTCC GAGGGCGGTG CGGCGTGGGG CACACCCGAG 
GCCTCGCAGG AGGAGCTGCG GTCACCTCGG GGCAGTGTGG CAGAGCGGCG TCGCCTGTTC 
CAGCAAAAGG CGGCCTCGTT GGATGAACGC ACGCGACAAC GCAGTGCAAC CTCGGACCTC 
GAACTCCGCT TCGCCCAGGA GCTGGGTCGC ATCCGCCGAT CTACGTCGCG GGAGGAGCTG 
GTGCGTTCGC ACGAGTCCCT GCGTGCCACG CTGCAGCGCG CCCCATCCCC TCGGGAGCCC 
GGCGAGCCCC CACTCTTCTC CCGGCCTTCC ACACCCAAGA CCTCACGGGC TGTGAGCCCG 
GCTGCCACCC AGCCGCCGCC TCCTAGTGGT GCGGGCAAAT CTGGGGACGA GCCTGGGAGG 
CCCCGAAGCA GAGGGCCGGT GGGCAGGACT GAACCGGGGG AAGGCCCGCA GCAGGAGATC 
AAGCGTCGGG ACCAATTCCC GCTAACCAGG AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT 
GTGCCGCCCT ACACCGCGGA TCCCCCGGAG AGCAGGACAA AAGCCCCCTC CGGTCGCAAG 
CGGGAACCCC CTGCTCAAGC GGTGCGCTTT CTGCCCTGGG CCACTCCGGG AGTGGAGGAC 
TCTGTTCTGC CCCAAACCTT GGAGAAGAAT AGAGCGGGAC CCGAGGCTGA GAAGAGGCTT 
CGCAGAGGAC CTGAGGAGGA TGGCCCCTGG GGGCCCTGGG ACCGCAGAGG GACCCGCAGC 
CAAGGCAAAG GTCGCCGTGC TCGGCCTACT TCCCCCGAGC TCGAGTCCTC AGACGACTCC 
TATGTGTCCG CTGGGGAAGA GCCCCTGGAG GCACCCGTGT TTGAGATCCC TCTGCAGAAT 
ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236949.1
CDS	1364..10687
Translation	MKKLWVKKRFQKTGHSRRAFGRLTHVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPSSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_011238647.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238647.2

ORF Insert Sequence:

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGCAGC 
ATTCCTCCGA GTGGATTGCA CAGAGAGGAG CCTGACCTTC AGCCTCAGCC AGCCAGTGAT 
GCCCTTCGTC CACGCCCTGC CCTTCCACCT CCCTCCAAAT CCGCTTTGCT TCCCCCACCA 
TCTCCTCGGG TGGGAAAGAG GGCTCTGCCA GGACCTAGTA CCCAGCCCCC AGCTACTCCC 
ACTTCGCCCC ATCGGCGTGC TCAGGAGCCC TCGCTGCCTG AGGACATCAC CACCACGGAA 
GAGAAGCGAG GGAAAAAGCC CAAGTCATCC GGGCCGTCGC TGGCGGGCAC AGTGGAGTCC 
CGGCCCCAGA CGCCACTGAG CGAGGCTTCG GGTCGCCTGT CAGCATTGGG CCGCTCGCCC 
CGGCTGGTGC GCGCGGGGTC CCGCATCCTG GACAAGCTAC AGTTCTTCGA AGAGCGGCGA 
CGCAGCCTGG AGCGCAGCGA CTCGCCGCCA GCGCCCCTGC GGCCCTGGGT GCCCCTGCGC 
AAGGCTCGCT CGCTGGAGCA GCCGAAGTCC GAGGGCGGTG CGGCGTGGGG CACACCCGAG 
GCCTCGCAGG AGGAGCTGCG GTCACCTCGG GGCAGTGTGG CAGAGCGGCG TCGCCTGTTC 
CAGCAAAAGG CGGCCTCGTT GGATGAACGC ACGCGACAAC GCAGTGCAAC CTCGGACCTC 
GAACTCCGCT TCGCCCAGGA GCTGGGTCGC ATCCGCCGAT CTACGTCGCG GGAGGAGCTG 
GTGCGTTCGC ACGAGTCCCT GCGTGCCACG CTGCAGCGCG CCCCATCCCC TCGGGAGCCC 
GGCGAGCCCC CACTCTTCTC CCGGCCTTCC ACACCCAAGA CCTCACGGGC TGTGAGCCCG 
GCTGCCACCC AGCCGCCGCC TCCTAGTGGT GCGGGCAAAT CTGGGGACGA GCCTGGGAGG 
CCCCGAAGCA GAGGGCCGGT GGGCAGGACT GAACCGGGGG AAGGCCCGCA GCAGGAGATC 
AAGCGTCGGG ACCAATTCCC GCTAACCAGG AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT 
GTGCCGCCCT ACACCGCGGA TCCCCCGGAG AGCAGGACAA AAGCCCCCTC CGGTCGCAAG 
CGGGAACCCC CTGCTCAAGC GGTGCGCTTT CTGCCCTGGG CCACTCCGGG AGTGGAGGAC 
TCTGTTCTGC CCCAAACCTT GGAGAAGAAT AGAGCGGGAC CCGAGGCTGA GAAGAGGCTT 
CGCAGAGGAC CTGAGGAGGA TGGCCCCTGG GGGCCCTGGG ACCGCAGAGG GACCCGCAGC 
CAAGGCAAAG GTCGCCGTGC TCGGCCTACT TCCCCCGAGC TCGAGTCCTC AGACGACTCC 
TATGTGTCCG CTGGGGAAGA GCCCCTGGAG GCACCCGTGT TTGAGATCCC TCTGCAGAAT 
ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_011238647.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236949.1 Mus musculus SPEG complex locus (Speg), transcript variant X7, mRNA.

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CloneID	OMu67148
Clone ID Related Accession (Same CDS sequence)	XM_011238647.2 , XM_011238647.1
Accession Version	XM_011238647.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGCAGC 
ATTCCTCCGA GTGGATTGCA CAGAGAGGAG CCTGACCTTC AGCCTCAGCC AGCCAGTGAT 
GCCCTTCGTC CACGCCCTGC CCTTCCACCT CCCTCCAAAT CCGCTTTGCT TCCCCCACCA 
TCTCCTCGGG TGGGAAAGAG GGCTCTGCCA GGACCTAGTA CCCAGCCCCC AGCTACTCCC 
ACTTCGCCCC ATCGGCGTGC TCAGGAGCCC TCGCTGCCTG AGGACATCAC CACCACGGAA 
GAGAAGCGAG GGAAAAAGCC CAAGTCATCC GGGCCGTCGC TGGCGGGCAC AGTGGAGTCC 
CGGCCCCAGA CGCCACTGAG CGAGGCTTCG GGTCGCCTGT CAGCATTGGG CCGCTCGCCC 
CGGCTGGTGC GCGCGGGGTC CCGCATCCTG GACAAGCTAC AGTTCTTCGA AGAGCGGCGA 
CGCAGCCTGG AGCGCAGCGA CTCGCCGCCA GCGCCCCTGC GGCCCTGGGT GCCCCTGCGC 
AAGGCTCGCT CGCTGGAGCA GCCGAAGTCC GAGGGCGGTG CGGCGTGGGG CACACCCGAG 
GCCTCGCAGG AGGAGCTGCG GTCACCTCGG GGCAGTGTGG CAGAGCGGCG TCGCCTGTTC 
CAGCAAAAGG CGGCCTCGTT GGATGAACGC ACGCGACAAC GCAGTGCAAC CTCGGACCTC 
GAACTCCGCT TCGCCCAGGA GCTGGGTCGC ATCCGCCGAT CTACGTCGCG GGAGGAGCTG 
GTGCGTTCGC ACGAGTCCCT GCGTGCCACG CTGCAGCGCG CCCCATCCCC TCGGGAGCCC 
GGCGAGCCCC CACTCTTCTC CCGGCCTTCC ACACCCAAGA CCTCACGGGC TGTGAGCCCG 
GCTGCCACCC AGCCGCCGCC TCCTAGTGGT GCGGGCAAAT CTGGGGACGA GCCTGGGAGG 
CCCCGAAGCA GAGGGCCGGT GGGCAGGACT GAACCGGGGG AAGGCCCGCA GCAGGAGATC 
AAGCGTCGGG ACCAATTCCC GCTAACCAGG AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT 
GTGCCGCCCT ACACCGCGGA TCCCCCGGAG AGCAGGACAA AAGCCCCCTC CGGTCGCAAG 
CGGGAACCCC CTGCTCAAGC GGTGCGCTTT CTGCCCTGGG CCACTCCGGG AGTGGAGGAC 
TCTGTTCTGC CCCAAACCTT GGAGAAGAAT AGAGCGGGAC CCGAGGCTGA GAAGAGGCTT 
CGCAGAGGAC CTGAGGAGGA TGGCCCCTGG GGGCCCTGGG ACCGCAGAGG GACCCGCAGC 
CAAGGCAAAG GTCGCCGTGC TCGGCCTACT TCCCCCGAGC TCGAGTCCTC AGACGACTCC 
TATGTGTCCG CTGGGGAAGA GCCCCTGGAG GCACCCGTGT TTGAGATCCC TCTGCAGAAT 
ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236949.1
CDS	65..9388
Translation	MKKLWVKKRFQKTGHSRRAFGRLTHVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPSSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_011238647.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238647.1

ORF Insert Sequence:

ATGAAGAAGC TGTGGGTGAA GAAGCGTTTC CAGAAAACCG GCCATTCCCG CCGGGCCTTT 
GGCCGACTTA CCCATGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGCAGC 
ATTCCTCCGA GTGGATTGCA CAGAGAGGAG CCTGACCTTC AGCCTCAGCC AGCCAGTGAT 
GCCCTTCGTC CACGCCCTGC CCTTCCACCT CCCTCCAAAT CCGCTTTGCT TCCCCCACCA 
TCTCCTCGGG TGGGAAAGAG GGCTCTGCCA GGACCTAGTA CCCAGCCCCC AGCTACTCCC 
ACTTCGCCCC ATCGGCGTGC TCAGGAGCCC TCGCTGCCTG AGGACATCAC CACCACGGAA 
GAGAAGCGAG GGAAAAAGCC CAAGTCATCC GGGCCGTCGC TGGCGGGCAC AGTGGAGTCC 
CGGCCCCAGA CGCCACTGAG CGAGGCTTCG GGTCGCCTGT CAGCATTGGG CCGCTCGCCC 
CGGCTGGTGC GCGCGGGGTC CCGCATCCTG GACAAGCTAC AGTTCTTCGA AGAGCGGCGA 
CGCAGCCTGG AGCGCAGCGA CTCGCCGCCA GCGCCCCTGC GGCCCTGGGT GCCCCTGCGC 
AAGGCTCGCT CGCTGGAGCA GCCGAAGTCC GAGGGCGGTG CGGCGTGGGG CACACCCGAG 
GCCTCGCAGG AGGAGCTGCG GTCACCTCGG GGCAGTGTGG CAGAGCGGCG TCGCCTGTTC 
CAGCAAAAGG CGGCCTCGTT GGATGAACGC ACGCGACAAC GCAGTGCAAC CTCGGACCTC 
GAACTCCGCT TCGCCCAGGA GCTGGGTCGC ATCCGCCGAT CTACGTCGCG GGAGGAGCTG 
GTGCGTTCGC ACGAGTCCCT GCGTGCCACG CTGCAGCGCG CCCCATCCCC TCGGGAGCCC 
GGCGAGCCCC CACTCTTCTC CCGGCCTTCC ACACCCAAGA CCTCACGGGC TGTGAGCCCG 
GCTGCCACCC AGCCGCCGCC TCCTAGTGGT GCGGGCAAAT CTGGGGACGA GCCTGGGAGG 
CCCCGAAGCA GAGGGCCGGT GGGCAGGACT GAACCGGGGG AAGGCCCGCA GCAGGAGATC 
AAGCGTCGGG ACCAATTCCC GCTAACCAGG AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT 
GTGCCGCCCT ACACCGCGGA TCCCCCGGAG AGCAGGACAA AAGCCCCCTC CGGTCGCAAG 
CGGGAACCCC CTGCTCAAGC GGTGCGCTTT CTGCCCTGGG CCACTCCGGG AGTGGAGGAC 
TCTGTTCTGC CCCAAACCTT GGAGAAGAAT AGAGCGGGAC CCGAGGCTGA GAAGAGGCTT 
CGCAGAGGAC CTGAGGAGGA TGGCCCCTGG GGGCCCTGGG ACCGCAGAGG GACCCGCAGC 
CAAGGCAAAG GTCGCCGTGC TCGGCCTACT TCCCCCGAGC TCGAGTCCTC AGACGACTCC 
TATGTGTCCG CTGGGGAAGA GCCCCTGGAG GCACCCGTGT TTGAGATCCC TCTGCAGAAT 
ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_017322354.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177843.1 Mus musculus SPEG complex locus (Speg), transcript variant X3, mRNA.

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CloneID	OMu76944
Clone ID Related Accession (Same CDS sequence)	XM_017322354.1 , XM_017322354.2
Accession Version	XM_017322354.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACC AGTGTTGGTA CCTTCTGGGG AGGGGGTCTC CCTCTCCTCC CCATCTCACA 
GAACATCTCT GGTATCCCTG GACAGCAGGG ACCCTCATGC CTCCCTATAT TCTGCCTGCC 
TTCCATGCCC CATCCCACAA ACACTCTGAT AACTGTCTGT CCACATCTCT CTCCTGCTGC 
TCCTATGGAA GCGAAGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA 
GCACAGACAA CCGGGGCCGG GCCACGGCAC CTGGGGGTGG AGCCGCTGGT GCGGGCATCG 
CGAGCTAATC TGGTGGGCGC AAGCTGGGGG TCAGAGGATA GCCTTTCGGT GGCCAGTGAC 
CTCTATGGCA GCGCGTTCAG TCTGTACAGA GGACGGGCGC TCTCGATCCA CGTCAGCATT 
CCTCCGAGTG GATTGCACAG AGAGGAGCCT GACCTTCAGC CTCAGCCAGC CAGTGATGCC 
CTTCGTCCAC GCCCTGCCCT TCCACCTCCC TCCAAATCCG CTTTGCTTCC CCCACCATCT 
CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA CCTAGTACCC AGCCCCCAGC TACTCCCACT 
TCGCCCCATC GGCGTGCTCA GGAGCCCTCG CTGCCTGAGG ACATCACCAC CACGGAAGAG 
AAGCGAGGGA AAAAGCCCAA GTCATCCGGG CCGTCGCTGG CGGGCACAGT GGAGTCCCGG 
CCCCAGACGC CACTGAGCGA GGCTTCGGGT CGCCTGTCAG CATTGGGCCG CTCGCCCCGG 
CTGGTGCGCG CGGGGTCCCG CATCCTGGAC AAGCTACAGT TCTTCGAAGA GCGGCGACGC 
AGCCTGGAGC GCAGCGACTC GCCGCCAGCG CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG 
GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC 
TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG 
CAAAAGGCGG CCTCGTTGGA TGAACGCACG CGACAACGCA GTGCAACCTC GGACCTCGAA 
CTCCGCTTCG CCCAGGAGCT GGGTCGCATC CGCCGATCTA CGTCGCGGGA GGAGCTGGTG 
CGTTCGCACG AGTCCCTGCG TGCCACGCTG CAGCGCGCCC CATCCCCTCG GGAGCCCGGC 
GAGCCCCCAC TCTTCTCCCG GCCTTCCACA CCCAAGACCT CACGGGCTGT GAGCCCGGCT 
GCCACCCAGC CGCCGCCTCC TAGTGGTGCG GGCAAATCTG GGGACGAGCC TGGGAGGCCC 
CGAAGCAGAG GGCCGGTGGG CAGGACTGAA CCGGGGGAAG GCCCGCAGCA GGAGATCAAG 
CGTCGGGACC AATTCCCGCT AACCAGGAGC AGAGCCATCC AGGAGTGCAG GAGCCCTGTG 
CCGCCCTACA CCGCGGATCC CCCGGAGAGC AGGACAAAAG CCCCCTCCGG TCGCAAGCGG 
GAACCCCCTG CTCAAGCGGT GCGCTTTCTG CCCTGGGCCA CTCCGGGAGT GGAGGACTCT 
GTTCTGCCCC AAACCTTGGA GAAGAATAGA GCGGGACCCG AGGCTGAGAA GAGGCTTCGC 
AGAGGACCTG AGGAGGATGG CCCCTGGGGG CCCTGGGACC GCAGAGGGAC CCGCAGCCAA 
GGCAAAGGTC GCCGTGCTCG GCCTACTTCC CCCGAGCTCG AGTCCTCAGA CGACTCCTAT 
GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA CCCGTGTTTG AGATCCCTCT GCAGAATATG 
GTGGTGGCGC CAGGAGCTGA CGTGCTACTT AAGTGTATCA TCACCGCCAA CCCCCCACCC 
CAAGTGTCCT GGAAAAAGGA TGGGTCCATG TTGCACAGCG AGGGTCGTCT TCTCATCCGG 
GCTGAAGGTG AACGGCACAC ACTGCTGCTC AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC 
TACACAGCCA CTGCCACCAA CGAACTGGGC CAAGCTACCT GTGCTTCTTC ACTGGCTGTG 
AGACCTGGCG GCTCCACATC CCCTTTCAGC AGCCCCATCA CCTCTGATGA GGAGTACCTG 
AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG GAGACCTGGC CCCGAACCCC TACCATGAAG 
CTCAGTCCCA GCCAGGATCA TGATTCCTCC GACTCTTCTT CCAAGGCACC CCCAACGTTC 
AAGGTCTCAC TCATGGACCA ATCGGTGAGA GAAGGTCAAG ATGTCATTAT GAGCATCCGT 
GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC TGGCTGAGGA ATCGACAGCC CGTGCGCCCA 
GACCAGCGGC GCTTTGCAGA GGAGGCCGAG GGTGGGCTCT GCCGCTTGAG GATCCTGGCT 
GCTGAACGGG GCGATGCTGG TTTCTACACA TGCAAGGCGG TCAACGAATA TGGCGCTCGG 
CAGTGCGAGG CCCGCCTGGA GGTCCGAGCA CACCCTGAAA GCCGGTCCCT GGCCGTGCTG 
GCCCCCCTGC AGGACGTGGA CGTGGGGGCC GGGGAGATGG CGCTGTTTGA GTGCCTGGTG 
GCAGGTCCAG CTGACGTCGA GGTGGACTGG CTGTGCCGGG GCCGCCTGCT GCAGCCTGCG 
CTGCTCAAAT GCAAGATGCA TTTTGATGGC CGCAAGTGCA AGCTGCTGCT CACCTCTGTG 
CACGAGGACG ACAGTGGTGT CTACACCTGC AAGCTGAGCA CAGCCAAAGA TGAACTGACC 
TGCAGTGCCC GGCTGACGGT TCGGCCGTCG CTGGCGCCTC TCTTCACCCG GTTGCTGGAA 
GATGTGGAGG TGTTGGAAGG CCGTGCTGCC CGCTTGGATT GCAAGATCAG CGGTACCCCG 
CCTCCCTCCG TTACCTGGAC TCATTTTGGC CACCCCGTGA ATGAGGGTGA CAACTTGCGG 
CTTCAACAGG ATGGAGGTCT ACACTCACTG CACATTGCCC GGGTGGGCAG TGAGGACGAA 
GGACTCTATG AAGTCAGTGC CACCAACACT CATGGCCAGG CCCACTGCTC CGCCCAGCTG 
TATGTGGAGG AGCCTCGGAC AGCTGCCTCT GGCCCCAGCT CGAAGCTGGA GAAGATGCCA 
TCTATCCCTG AGGAGCCGGA GCACGGAGAT CTAGAGAGGC TGTCTATTCC TGACTTCCTA 
CGGCCACTGC AAGATCTGGA AGTGGGACTA GCTAAGGAGG CCATGCTGGA GTGCCAAGTG 
ACCGGCCTGC CCTATCCCAC CATCAGCTGG TTCCACAATG GCCACCGCAT TCAAAGCAGT 
GACGACCGAC GCATGACACA GTACAGGGAC ATACATCGCC TGGTGTTCCC AGCTGTGGGG 
CCTCAGCATG CTGGTGTCTA TAAGAGTGTA ATCGCCAACA AGCTGGGCAA AGCGGCCTGC 
TACGCCCATC TCTATGTCAC AGATGTGGTT CCAGGCCCTC CAGATGGTGC TCCGGAAGTA 
GTAGCTGTGA CCGGAAGGAT GGTCACACTA TCGTGGAACC CACCCAGGAG TCTGGACATG 
GCTATTGACC CAGATTCCCT AACATACACT GTGCAGCACC AGGTGTTGGG CTCGGACCAG 
TGGACGGCAC TGGTCACAGG CCTGCGGGAG CCTGCGTGGG CAGCCACAGG GCTGAAGAAG 
GGGATCCAGC ACATCTTCCG GGTCCTCAGC TCCAGTGGCA AGAGCAGCAG CAAGCCCTCA 
GCACCGTCGG AACCCGTGCA GCTGCTAGAG CATGGCCCAC CCCTGGAAGA GGCGCCTGCT 
GTGCTGGACA AGCAGGACAT TGTGTATGTG GTAGAGGGCC AGCCTGCCTG TGTCACCGTC 
ACTTTTAACC ATGTGGAGGC CCAGGTTGTC TGGAGGAGCT GCCGGGGAGC CCTCCTCGAG 
GCGCGCACAG GTGTGTATGA ACTGAGTCAG CCAGATGACG ACCAGTATTG TCTGCGGATC 
TGCCGGGTGA GCCGACGAGA CCTGGGACCC CTTACTTGCA GTGCCCGAAA CCGCCACGGC 
ACAAAGGCCT GCTCGGTCAC CCTGGAGCTG GCAGAGGCAC CTCGGTTTGA GTCTATCATG 
GAAGATGTGG AGGTGGGGCC TGGAGAAACC GCTCGCTTTG CTGTGGTAGT TGAGGGGAAA 
CCACTGCCAG ACATCATGTG GTACAAGGAT GAGGTGCTGC TGGCCGAGAG CAACCATGTG 
AGCTTCGTGT ATGAGGAGAA CGAGTGTTCC CTAGTGCTGC TCAGCGCAGG AAGCCAGGAT 
GGAGGCGTCT ATACCTGTAC CGCCCGGAAC CTGGCAGGCG AAGTCTCCTG CAAGGCGGAG 
CTGTCTGTGC TTTCAGCTCA GACAGCCATG GAGGTCGAGG GGGTCGGAGA GGATGAGGAA 
CACCGAGGAA GAAGACTTAG CGACTACTAC GACATCCACC AAGAGATTGG CAGGGGTGCC 
TTCTCCTACC TGCGGCGTGT AGTGGAGAGG AGCTCTGGCC TGGAGTTTGC AGCCAAGTTC 
ATCCCTAGCC AGGCTAAGCC TAAAGCATCA GCAAGACGCG AGGCGCGCTT GCTGGCCCGG 
CTCCAGCATG GCTGTGTGCT CTACTTCCAC GAAGCCTTTG AGAGGCGCAG GGGACTTGTC 
ATAGTCACGG AGCTCTGCAC AGAAGAGCTT CTAGAGCGCA TGGCCAGGAA GCCCACCGTG 
TGTGAGTCTG AGACTCGGAC CTATATGCGT CAAGTGCTAG AGGGGATATG CTATCTGCAT 
CAGAGCCATG TGCTGCACCT GGATGTCAAG CCGGAGAACC TGCTTGTGTG GGATGGTGCA 
GGGGGTGAAG AGCAGGTGCG CATCTGTGAC TTCGGGAACG CCCAGGAACT CACTCCGGGA 
GAGCCCCAGT ACTGTCAGTA TGGCACGCCA GAGTTTGTAG CACCTGAGAT TGTCAACCAG 
AGCCCTGTGT CTGGAGTCAC TGACATCTGG CCCGTGGGCG TCGTTGCCTT TCTCTGTTTG 
ACGGGCATCT CCCCATTTGT TGGGGAGAAT GACCGGACAA CACTCATGAA TATCCGAAAC 
TACAACGTGG CATTTGAGGA GACTACGTTC CTGAGTCTGA GCAGGGAGGC CCGGGGCTTC 
CTCATCAAAG TGCTGGTGCA GGATCGACTG AGACCTACTG CAGAAGAGAC ATTGGAACAT 
CCTTGGTTCA AAACAGAAGC AAAGGGTGCA GAGGTGAGCA CAGATCACCT GAAGCTCTTC 
CTGTCACGGA GGAGGTGGCA GCGCTCTCAA ATCAGCTACA AGTGCCACCT GGTGCTGCGC 
CCCATTCCGG AACTGCTACG GGCTCCCCCG GAGCGGGTGT GGGTGGCCAT GCCTAGAAGA 
CAACCCCCGA GTGGGGGACT CTCATCCTCT TCAGACTCAG AAGAGGAAGA GTTGGAGGAA 
CTTCCCTCAG TACCCCGCCC CCTGCAGCCT GAGTTCTCAG GTTCTCGGGT ATCCCTCACC 
GACATCCCCA CCGAAGATGA GGCTCTGGGG ACCCCGGAAG CTGGGGCTGC CACCCCCATG 
GACTGGCAAG AGCAGGAAAG GACTCCCTCT AAGGACCAGG AGGCCCCCAG CCCTGAGGCA 
CTCCCCTCTC CAGGCCAGGA GTCCCCTGAT GGGCCTAGCC CCAGGCGGCC AGAGCTCCGC 
AGGGGTAGCT CTGCTGAGAG TGCCCTGCCC CGAGTCGGGT CGCGGGAACC GGGCCGGAGC 
TTGCACAAGG CAGCATCCGT GGAGCTGCCG CAGCGCAGGA GCCCCAGCCC GGGTGCCACC 
CGCCTGACTC GGGGAGGCCT GGGTGAGGGC GAGTATGCCC AGAGGCTGCA GGCCCTGCGT 
CAGCGGCTGC TGAGGGGAGG CCCCGAGGAT GGCAAGGTCA GCGGCCTCAG GGGACCCCTG 
CTGGAGAGCC TGGGGGGCCG TGCACGGGAT CCCAGGATGG CAAGGGCTGC CTCCAGCGAG 
GCTGCCCCCC ACCACCAGCC CCCTCCGGAG TCCCGGGGTC TGCAAAAGAG CAGCAGCTTC 
TCTCAGGGCG AGGCAGAGCC CCGGGGTCGG CACCGCCGCG CGGGGGCACC CCTCGAAATC 
CCCGTGGCCA GACTTGGGGC TCGCAGGCTG CAGGAGTCTC CCTCCTTATC TGCCCTTAGC 
GAGACCCAGC CACCGAGCCC TGCGAGGCCC AGCGTCCCCA AACTCAGTAT CACCAAGTCC 
CCGGAACCTT CCGCTGTCAC ATCCAGAGAC TCTCCCCAGC CCCCGGAACC TCAGCCTGTC 
CCAGAAAAGG TCCCTGAGCC CAAACCAGAA CCAGTCCGAG CTGCAAAACC AGCACAACCC 
CCTTTGGCTC TGCAAATGCC AACGCAGCCT CTCACCCCCT ATGCCCAGAT CATGCAGTCG 
CTCCAGCTGT CCAGTCCCAC CCTGTCCCCA CAGGACCCCG CTGTGCCTCC ATCAGAGCCC 
AAGCCCCACG CGGCTGTGTT TGCCAGGGTG GCTTCCCCAC CACCCGGAGT CTCTGAGAAG 
CGCGTGCCTT CGGCCAGAAC TCCCCCAGTG CTAGCCGAGA AAGCCCGAGT CCCCACGGTG 
CCCCCCAGGC CAGGCAGCAG CCTCAGCGGC AGCATCGAAA ACCTTGAATC AGAGGCTGTG 
TTCGAGGCTA AGTTCAAACG CAGCCGCGAA TCGCCACTGT CGCGGGGCCT GAGGCTACTG 
AGCCGCTCGC GCTCGGAGGA GCGCGGTCCC TTCCGTGGGG CGGAGGACGA CGGCATATAC 
CGGCCCAGTC CGGCAGGAAC GCCGCTAGAG CTGGTGCGAC GGCCCGAGCG CTCGCGCTCC 
GTGCAGGACC TCAGGGTAGC CGGAGAGCCG GGCCTTGTGC GTCGCCTTTC GCTGTCCCTG 
TCGCAGAAGC TGCGGCGGAC ACCGCCGGGA CAGCGACACC CTGCCTGGGA GTCCCGCAGC 
GGAGACGGGG AGAGCTCCGA GGGCGGCAGC TCAGCTCGGG CATCCCCAGT GCTGGCGGTA 
CGCAGGAGAC TCAGCTCCAC GCTGGAGCGT CTGTCCAGCC GTTTGCAGCG CAGCGGCAGC 
AGCGAGGACT CCGGGGGCGC CTCGGGTCGC AGCACGCCGC TGTTTGGACG GCTGCGCAGG 
GCTACTTCGG AAGGGGAGAG CCTGCGACGC CTCGGTGTTC CGCACAATCA GCTGGGCTCT 
CAGACTGGTG CCACTACACC TTCCGCTGAG TCTCTGGGCT CAGAGGCCAG TGGCACATCG 
GGTTCTTCAG CTCCTGGAGA AAGCCGAAGC CGGCACCGGT GGGGCCTTTC CAGGCTGCGC 
AAGGACAAGG GATTATCTCA GCCAAACCTC TCCTCCAGTG TCCAAGAGGA CTTGGGTCAC 
CAGTATGTGC CCAGTGAGTC AGATTTCCCC CCAGTCTTCC ACATCAAACT CAAGGACCAG 
GTGCTGTTGG AGGGGGAAGC AGCCACCCTG CTCTGCCTAC CCGCAGCCTG CCCAGCACCC 
CGCATCTCCT GGATGAAAGA TAAGCAGTCC CTGCGTTCAG AGCCCTCAGT GGTCATTGTG 
TCCTGCAAAG ATGGACGGCA GCTGCTGAGC ATACCCCGGG CCGGCAAGCG GCATGCTGGG 
CTCTATGAGT GCTCAGCCAC CAATGTCCTG GGCAGCATTA CCAGCTCCTG TACTGTGGCC 
GTGGCCCGCA TTCCAGGGAA GCTAGCTCCT CCAGAGGTGC CCCAGACCTA CCATGACACA 
GCACTAGTAG TGTGGAAGCC AGGAGACGGT CGGGCACCTT GCACATACAC TCTGGAGCGG 
CGGGTGGATG GGGAATCTGT CTGGCACCCT GTGAGCTCAG GCATCCCCGA CTGTTATTAC 
AATGTGACTC AGCTGCCTGT CGGCGTGACC GTGCGGTTCC GTGTGGCCTG TTCCAATCGT 
GCTGGGCAGG GGCCCTTCAG CAACCCTTCT GAGAAGGTCT TCATCCGAGG CACTCCAGAT 
TCTCCTGCTC AGCCAGCTGC TGCTCCCCGA GATGCCCCTG TTACCTCAGG GCCAACCAGG 
GCCCCACCGC CTGACTCTCC TACCTCACTG GCCCCCACCC CCGCTCTTGC GCCCCCAGCC 
TCCCAGGCAT CCACTCTCAG CCCCTCAACT TCCTCCATGT CTGCCAACCA GGCCTTGTCC 
TCACTCAAGG CTGTGGGTCC ACCACCCGCA ACCCCTCCAC GAAAGCACAG GGGCCTTCTG 
GCCACCCAGC AAGCAGAGCC ATCCCCACCC AGTATCGTGG TCACCCCAAG TGAACCCAGG 
TCTTTTGTCC CTGACACTGG AACCCTGACC CCAACCTCTA GTCCTCAAGG GGTTAAACCA 
GCACCTTCCT CAACTTCCTT GTATATGGTG ACTTCCTTCG TGTCTGCACC TCCAGCCCCC 
CAGGCCCCAG CCCCTGAGCC CCCTCCTGAG CCCACCAAGG TGACTGTGCG GAGCCTCAGC 
CCAGCCAAGG AGGTGGTCAG CTCCCCAACT CCTGAGAGCA CCACTCTTCG GCAGGGTCCC 
CCTCAGAAAC CCTACACCTT CCTGGAGGAG AAGGCCAGGG GCCGCTTTGG CGTTGTGCGG 
TCATGCCGGG AGAATGCTAC GGGCCGAACG TTTGTCGCCA AGATCGTGCC GTACGCCGCG 
GAGGGCAAGC GACGCGTGCT GCAGGAATAC GAGGTGCTTC GGACGCTGCA CCACGAGCGG 
CTCATGTCCC TGCACGAGGC CTACATCACC CCTCGGTACC TCGTGCTTAT CGCTGAGAGC 
TGTGGCAACC GGGAACTCCT CTGTGGGCTC AGCGACAGAT TCCGGTATTC AGAAGATGAC 
GTGGCCACCT ATGTGGTGCA GCTGCTACAA GGCCTGGACT ACCTCCACGG CCACCACGTG 
CTACACCTGG ACATCAAGCC GGACAATCTA CTGTTGGCCG CTGACAATGC CCTTAAAATT 
GTGGACTTCG GCAGTGCTCA GCCCTATAAC CCTCAGGCCC TGAAGCCACT TGGCCACCGC 
ACAGGCACTC TGGAGTTCAT GGCTCCTGAG ATGGTGAAGG GAGACCCCAT TGGCTCTGCC 
ACGGACATCT GGGGAGCGGG TGTGCTCACC TACATCATGC TTAGTGGGTA CTCCCCATTC 
TATGAGCCAG ACCCCCAGGA AACAGAGGCT CGGATTGTTG GGGGTCGCTT TGATGCCTTC 
CAGTTGTATC CTAACACATC CCAGAGTGCC ACCCTCTTCT TGAGAAAGGT CCTCTCAGTA 
CATCCCTGGA GCCGGCCCTC TCTGCAGGAC TGCTTGGCCC ACCCATGGCT GCAAGATGCC 
TACCTGATGA AGCTGCGCCG CCAGACACTC ACCTTCACCA CCAACCGGCT CAAGGAATTC 
CTGGGCGAGC AGCGGCGACG TCGGGCTGAG GCTGCTACCC GTCACAAGGT GCTGCTCCGC 
TCCTACCCTG GCAGCCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177843.1
CDS	764..10384
Translation	MAHQCWYLLGRGSPSPPHLTEHLWYPWTAGTLMPPYILPAFHAPSHKHSDNCLSTSLSCCSYGSEVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPRQAQTTGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_017322354.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322354.1

ORF Insert Sequence:

ATGGCTCACC AGTGTTGGTA CCTTCTGGGG AGGGGGTCTC CCTCTCCTCC CCATCTCACA 
GAACATCTCT GGTATCCCTG GACAGCAGGG ACCCTCATGC CTCCCTATAT TCTGCCTGCC 
TTCCATGCCC CATCCCACAA ACACTCTGAT AACTGTCTGT CCACATCTCT CTCCTGCTGC 
TCCTATGGAA GCGAAGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA 
GCACAGACAA CCGGGGCCGG GCCACGGCAC CTGGGGGTGG AGCCGCTGGT GCGGGCATCG 
CGAGCTAATC TGGTGGGCGC AAGCTGGGGG TCAGAGGATA GCCTTTCGGT GGCCAGTGAC 
CTCTATGGCA GCGCGTTCAG TCTGTACAGA GGACGGGCGC TCTCGATCCA CGTCAGCATT 
CCTCCGAGTG GATTGCACAG AGAGGAGCCT GACCTTCAGC CTCAGCCAGC CAGTGATGCC 
CTTCGTCCAC GCCCTGCCCT TCCACCTCCC TCCAAATCCG CTTTGCTTCC CCCACCATCT 
CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA CCTAGTACCC AGCCCCCAGC TACTCCCACT 
TCGCCCCATC GGCGTGCTCA GGAGCCCTCG CTGCCTGAGG ACATCACCAC CACGGAAGAG 
AAGCGAGGGA AAAAGCCCAA GTCATCCGGG CCGTCGCTGG CGGGCACAGT GGAGTCCCGG 
CCCCAGACGC CACTGAGCGA GGCTTCGGGT CGCCTGTCAG CATTGGGCCG CTCGCCCCGG 
CTGGTGCGCG CGGGGTCCCG CATCCTGGAC AAGCTACAGT TCTTCGAAGA GCGGCGACGC 
AGCCTGGAGC GCAGCGACTC GCCGCCAGCG CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG 
GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC 
TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG 
CAAAAGGCGG CCTCGTTGGA TGAACGCACG CGACAACGCA GTGCAACCTC GGACCTCGAA 
CTCCGCTTCG CCCAGGAGCT GGGTCGCATC CGCCGATCTA CGTCGCGGGA GGAGCTGGTG 
CGTTCGCACG AGTCCCTGCG TGCCACGCTG CAGCGCGCCC CATCCCCTCG GGAGCCCGGC 
GAGCCCCCAC TCTTCTCCCG GCCTTCCACA CCCAAGACCT CACGGGCTGT GAGCCCGGCT 
GCCACCCAGC CGCCGCCTCC TAGTGGTGCG GGCAAATCTG GGGACGAGCC TGGGAGGCCC 
CGAAGCAGAG GGCCGGTGGG CAGGACTGAA CCGGGGGAAG GCCCGCAGCA GGAGATCAAG 
CGTCGGGACC AATTCCCGCT AACCAGGAGC AGAGCCATCC AGGAGTGCAG GAGCCCTGTG 
CCGCCCTACA CCGCGGATCC CCCGGAGAGC AGGACAAAAG CCCCCTCCGG TCGCAAGCGG 
GAACCCCCTG CTCAAGCGGT GCGCTTTCTG CCCTGGGCCA CTCCGGGAGT GGAGGACTCT 
GTTCTGCCCC AAACCTTGGA GAAGAATAGA GCGGGACCCG AGGCTGAGAA GAGGCTTCGC 
AGAGGACCTG AGGAGGATGG CCCCTGGGGG CCCTGGGACC GCAGAGGGAC CCGCAGCCAA 
GGCAAAGGTC GCCGTGCTCG GCCTACTTCC CCCGAGCTCG AGTCCTCAGA CGACTCCTAT 
GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA CCCGTGTTTG AGATCCCTCT GCAGAATATG 
GTGGTGGCGC CAGGAGCTGA CGTGCTACTT AAGTGTATCA TCACCGCCAA CCCCCCACCC 
CAAGTGTCCT GGAAAAAGGA TGGGTCCATG TTGCACAGCG AGGGTCGTCT TCTCATCCGG 
GCTGAAGGTG AACGGCACAC ACTGCTGCTC AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC 
TACACAGCCA CTGCCACCAA CGAACTGGGC CAAGCTACCT GTGCTTCTTC ACTGGCTGTG 
AGACCTGGCG GCTCCACATC CCCTTTCAGC AGCCCCATCA CCTCTGATGA GGAGTACCTG 
AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG GAGACCTGGC CCCGAACCCC TACCATGAAG 
CTCAGTCCCA GCCAGGATCA TGATTCCTCC GACTCTTCTT CCAAGGCACC CCCAACGTTC 
AAGGTCTCAC TCATGGACCA ATCGGTGAGA GAAGGTCAAG ATGTCATTAT GAGCATCCGT 
GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC TGGCTGAGGA ATCGACAGCC CGTGCGCCCA 
GACCAGCGGC GCTTTGCAGA GGAGGCCGAG GGTGGGCTCT GCCGCTTGAG GATCCTGGCT 
GCTGAACGGG GCGATGCTGG TTTCTACACA TGCAAGGCGG TCAACGAATA TGGCGCTCGG 
CAGTGCGAGG CCCGCCTGGA GGTCCGAGCA CACCCTGAAA GCCGGTCCCT GGCCGTGCTG 
GCCCCCCTGC AGGACGTGGA CGTGGGGGCC GGGGAGATGG CGCTGTTTGA GTGCCTGGTG 
GCAGGTCCAG CTGACGTCGA GGTGGACTGG CTGTGCCGGG GCCGCCTGCT GCAGCCTGCG 
CTGCTCAAAT GCAAGATGCA TTTTGATGGC CGCAAGTGCA AGCTGCTGCT CACCTCTGTG 
CACGAGGACG ACAGTGGTGT CTACACCTGC AAGCTGAGCA CAGCCAAAGA TGAACTGACC 
TGCAGTGCCC GGCTGACGGT TCGGCCGTCG CTGGCGCCTC TCTTCACCCG GTTGCTGGAA 
GATGTGGAGG TGTTGGAAGG CCGTGCTGCC CGCTTGGATT GCAAGATCAG CGGTACCCCG 
CCTCCCTCCG TTACCTGGAC TCATTTTGGC CACCCCGTGA ATGAGGGTGA CAACTTGCGG 
CTTCAACAGG ATGGAGGTCT ACACTCACTG CACATTGCCC GGGTGGGCAG TGAGGACGAA 
GGACTCTATG AAGTCAGTGC CACCAACACT CATGGCCAGG CCCACTGCTC CGCCCAGCTG 
TATGTGGAGG AGCCTCGGAC AGCTGCCTCT GGCCCCAGCT CGAAGCTGGA GAAGATGCCA 
TCTATCCCTG AGGAGCCGGA GCACGGAGAT CTAGAGAGGC TGTCTATTCC TGACTTCCTA 
CGGCCACTGC AAGATCTGGA AGTGGGACTA GCTAAGGAGG CCATGCTGGA GTGCCAAGTG 
ACCGGCCTGC CCTATCCCAC CATCAGCTGG TTCCACAATG GCCACCGCAT TCAAAGCAGT 
GACGACCGAC GCATGACACA GTACAGGGAC ATACATCGCC TGGTGTTCCC AGCTGTGGGG 
CCTCAGCATG CTGGTGTCTA TAAGAGTGTA ATCGCCAACA AGCTGGGCAA AGCGGCCTGC 
TACGCCCATC TCTATGTCAC AGATGTGGTT CCAGGCCCTC CAGATGGTGC TCCGGAAGTA 
GTAGCTGTGA CCGGAAGGAT GGTCACACTA TCGTGGAACC CACCCAGGAG TCTGGACATG 
GCTATTGACC CAGATTCCCT AACATACACT GTGCAGCACC AGGTGTTGGG CTCGGACCAG 
TGGACGGCAC TGGTCACAGG CCTGCGGGAG CCTGCGTGGG CAGCCACAGG GCTGAAGAAG 
GGGATCCAGC ACATCTTCCG GGTCCTCAGC TCCAGTGGCA AGAGCAGCAG CAAGCCCTCA 
GCACCGTCGG AACCCGTGCA GCTGCTAGAG CATGGCCCAC CCCTGGAAGA GGCGCCTGCT 
GTGCTGGACA AGCAGGACAT TGTGTATGTG GTAGAGGGCC AGCCTGCCTG TGTCACCGTC 
ACTTTTAACC ATGTGGAGGC CCAGGTTGTC TGGAGGAGCT GCCGGGGAGC CCTCCTCGAG 
GCGCGCACAG GTGTGTATGA ACTGAGTCAG CCAGATGACG ACCAGTATTG TCTGCGGATC 
TGCCGGGTGA GCCGACGAGA CCTGGGACCC CTTACTTGCA GTGCCCGAAA CCGCCACGGC 
ACAAAGGCCT GCTCGGTCAC CCTGGAGCTG GCAGAGGCAC CTCGGTTTGA GTCTATCATG 
GAAGATGTGG AGGTGGGGCC TGGAGAAACC GCTCGCTTTG CTGTGGTAGT TGAGGGGAAA 
CCACTGCCAG ACATCATGTG GTACAAGGAT GAGGTGCTGC TGGCCGAGAG CAACCATGTG 
AGCTTCGTGT ATGAGGAGAA CGAGTGTTCC CTAGTGCTGC TCAGCGCAGG AAGCCAGGAT 
GGAGGCGTCT ATACCTGTAC CGCCCGGAAC CTGGCAGGCG AAGTCTCCTG CAAGGCGGAG 
CTGTCTGTGC TTTCAGCTCA GACAGCCATG GAGGTCGAGG GGGTCGGAGA GGATGAGGAA 
CACCGAGGAA GAAGACTTAG CGACTACTAC GACATCCACC AAGAGATTGG CAGGGGTGCC 
TTCTCCTACC TGCGGCGTGT AGTGGAGAGG AGCTCTGGCC TGGAGTTTGC AGCCAAGTTC 
ATCCCTAGCC AGGCTAAGCC TAAAGCATCA GCAAGACGCG AGGCGCGCTT GCTGGCCCGG 
CTCCAGCATG GCTGTGTGCT CTACTTCCAC GAAGCCTTTG AGAGGCGCAG GGGACTTGTC 
ATAGTCACGG AGCTCTGCAC AGAAGAGCTT CTAGAGCGCA TGGCCAGGAA GCCCACCGTG 
TGTGAGTCTG AGACTCGGAC CTATATGCGT CAAGTGCTAG AGGGGATATG CTATCTGCAT 
CAGAGCCATG TGCTGCACCT GGATGTCAAG CCGGAGAACC TGCTTGTGTG GGATGGTGCA 
GGGGGTGAAG AGCAGGTGCG CATCTGTGAC TTCGGGAACG CCCAGGAACT CACTCCGGGA 
GAGCCCCAGT ACTGTCAGTA TGGCACGCCA GAGTTTGTAG CACCTGAGAT TGTCAACCAG 
AGCCCTGTGT CTGGAGTCAC TGACATCTGG CCCGTGGGCG TCGTTGCCTT TCTCTGTTTG 
ACGGGCATCT CCCCATTTGT TGGGGAGAAT GACCGGACAA CACTCATGAA TATCCGAAAC 
TACAACGTGG CATTTGAGGA GACTACGTTC CTGAGTCTGA GCAGGGAGGC CCGGGGCTTC 
CTCATCAAAG TGCTGGTGCA GGATCGACTG AGACCTACTG CAGAAGAGAC ATTGGAACAT 
CCTTGGTTCA AAACAGAAGC AAAGGGTGCA GAGGTGAGCA CAGATCACCT GAAGCTCTTC 
CTGTCACGGA GGAGGTGGCA GCGCTCTCAA ATCAGCTACA AGTGCCACCT GGTGCTGCGC 
CCCATTCCGG AACTGCTACG GGCTCCCCCG GAGCGGGTGT GGGTGGCCAT GCCTAGAAGA 
CAACCCCCGA GTGGGGGACT CTCATCCTCT TCAGACTCAG AAGAGGAAGA GTTGGAGGAA 
CTTCCCTCAG TACCCCGCCC CCTGCAGCCT GAGTTCTCAG GTTCTCGGGT ATCCCTCACC 
GACATCCCCA CCGAAGATGA GGCTCTGGGG ACCCCGGAAG CTGGGGCTGC CACCCCCATG 
GACTGGCAAG AGCAGGAAAG GACTCCCTCT AAGGACCAGG AGGCCCCCAG CCCTGAGGCA 
CTCCCCTCTC CAGGCCAGGA GTCCCCTGAT GGGCCTAGCC CCAGGCGGCC AGAGCTCCGC 
AGGGGTAGCT CTGCTGAGAG TGCCCTGCCC CGAGTCGGGT CGCGGGAACC GGGCCGGAGC 
TTGCACAAGG CAGCATCCGT GGAGCTGCCG CAGCGCAGGA GCCCCAGCCC GGGTGCCACC 
CGCCTGACTC GGGGAGGCCT GGGTGAGGGC GAGTATGCCC AGAGGCTGCA GGCCCTGCGT 
CAGCGGCTGC TGAGGGGAGG CCCCGAGGAT GGCAAGGTCA GCGGCCTCAG GGGACCCCTG 
CTGGAGAGCC TGGGGGGCCG TGCACGGGAT CCCAGGATGG CAAGGGCTGC CTCCAGCGAG 
GCTGCCCCCC ACCACCAGCC CCCTCCGGAG TCCCGGGGTC TGCAAAAGAG CAGCAGCTTC 
TCTCAGGGCG AGGCAGAGCC CCGGGGTCGG CACCGCCGCG CGGGGGCACC CCTCGAAATC 
CCCGTGGCCA GACTTGGGGC TCGCAGGCTG CAGGAGTCTC CCTCCTTATC TGCCCTTAGC 
GAGACCCAGC CACCGAGCCC TGCGAGGCCC AGCGTCCCCA AACTCAGTAT CACCAAGTCC 
CCGGAACCTT CCGCTGTCAC ATCCAGAGAC TCTCCCCAGC CCCCGGAACC TCAGCCTGTC 
CCAGAAAAGG TCCCTGAGCC CAAACCAGAA CCAGTCCGAG CTGCAAAACC AGCACAACCC 
CCTTTGGCTC TGCAAATGCC AACGCAGCCT CTCACCCCCT ATGCCCAGAT CATGCAGTCG 
CTCCAGCTGT CCAGTCCCAC CCTGTCCCCA CAGGACCCCG CTGTGCCTCC ATCAGAGCCC 
AAGCCCCACG CGGCTGTGTT TGCCAGGGTG GCTTCCCCAC CACCCGGAGT CTCTGAGAAG 
CGCGTGCCTT CGGCCAGAAC TCCCCCAGTG CTAGCCGAGA AAGCCCGAGT CCCCACGGTG 
CCCCCCAGGC CAGGCAGCAG CCTCAGCGGC AGCATCGAAA ACCTTGAATC AGAGGCTGTG 
TTCGAGGCTA AGTTCAAACG CAGCCGCGAA TCGCCACTGT CGCGGGGCCT GAGGCTACTG 
AGCCGCTCGC GCTCGGAGGA GCGCGGTCCC TTCCGTGGGG CGGAGGACGA CGGCATATAC 
CGGCCCAGTC CGGCAGGAAC GCCGCTAGAG CTGGTGCGAC GGCCCGAGCG CTCGCGCTCC 
GTGCAGGACC TCAGGGTAGC CGGAGAGCCG GGCCTTGTGC GTCGCCTTTC GCTGTCCCTG 
TCGCAGAAGC TGCGGCGGAC ACCGCCGGGA CAGCGACACC CTGCCTGGGA GTCCCGCAGC 
GGAGACGGGG AGAGCTCCGA GGGCGGCAGC TCAGCTCGGG CATCCCCAGT GCTGGCGGTA 
CGCAGGAGAC TCAGCTCCAC GCTGGAGCGT CTGTCCAGCC GTTTGCAGCG CAGCGGCAGC 
AGCGAGGACT CCGGGGGCGC CTCGGGTCGC AGCACGCCGC TGTTTGGACG GCTGCGCAGG 
GCTACTTCGG AAGGGGAGAG CCTGCGACGC CTCGGTGTTC CGCACAATCA GCTGGGCTCT 
CAGACTGGTG CCACTACACC TTCCGCTGAG TCTCTGGGCT CAGAGGCCAG TGGCACATCG 
GGTTCTTCAG CTCCTGGAGA AAGCCGAAGC CGGCACCGGT GGGGCCTTTC CAGGCTGCGC 
AAGGACAAGG GATTATCTCA GCCAAACCTC TCCTCCAGTG TCCAAGAGGA CTTGGGTCAC 
CAGTATGTGC CCAGTGAGTC AGATTTCCCC CCAGTCTTCC ACATCAAACT CAAGGACCAG 
GTGCTGTTGG AGGGGGAAGC AGCCACCCTG CTCTGCCTAC CCGCAGCCTG CCCAGCACCC 
CGCATCTCCT GGATGAAAGA TAAGCAGTCC CTGCGTTCAG AGCCCTCAGT GGTCATTGTG 
TCCTGCAAAG ATGGACGGCA GCTGCTGAGC ATACCCCGGG CCGGCAAGCG GCATGCTGGG 
CTCTATGAGT GCTCAGCCAC CAATGTCCTG GGCAGCATTA CCAGCTCCTG TACTGTGGCC 
GTGGCCCGCA TTCCAGGGAA GCTAGCTCCT CCAGAGGTGC CCCAGACCTA CCATGACACA 
GCACTAGTAG TGTGGAAGCC AGGAGACGGT CGGGCACCTT GCACATACAC TCTGGAGCGG 
CGGGTGGATG GGGAATCTGT CTGGCACCCT GTGAGCTCAG GCATCCCCGA CTGTTATTAC 
AATGTGACTC AGCTGCCTGT CGGCGTGACC GTGCGGTTCC GTGTGGCCTG TTCCAATCGT 
GCTGGGCAGG GGCCCTTCAG CAACCCTTCT GAGAAGGTCT TCATCCGAGG CACTCCAGAT 
TCTCCTGCTC AGCCAGCTGC TGCTCCCCGA GATGCCCCTG TTACCTCAGG GCCAACCAGG 
GCCCCACCGC CTGACTCTCC TACCTCACTG GCCCCCACCC CCGCTCTTGC GCCCCCAGCC 
TCCCAGGCAT CCACTCTCAG CCCCTCAACT TCCTCCATGT CTGCCAACCA GGCCTTGTCC 
TCACTCAAGG CTGTGGGTCC ACCACCCGCA ACCCCTCCAC GAAAGCACAG GGGCCTTCTG 
GCCACCCAGC AAGCAGAGCC ATCCCCACCC AGTATCGTGG TCACCCCAAG TGAACCCAGG 
TCTTTTGTCC CTGACACTGG AACCCTGACC CCAACCTCTA GTCCTCAAGG GGTTAAACCA 
GCACCTTCCT CAACTTCCTT GTATATGGTG ACTTCCTTCG TGTCTGCACC TCCAGCCCCC 
CAGGCCCCAG CCCCTGAGCC CCCTCCTGAG CCCACCAAGG TGACTGTGCG GAGCCTCAGC 
CCAGCCAAGG AGGTGGTCAG CTCCCCAACT CCTGAGAGCA CCACTCTTCG GCAGGGTCCC 
CCTCAGAAAC CCTACACCTT CCTGGAGGAG AAGGCCAGGG GCCGCTTTGG CGTTGTGCGG 
TCATGCCGGG AGAATGCTAC GGGCCGAACG TTTGTCGCCA AGATCGTGCC GTACGCCGCG 
GAGGGCAAGC GACGCGTGCT GCAGGAATAC GAGGTGCTTC GGACGCTGCA CCACGAGCGG 
CTCATGTCCC TGCACGAGGC CTACATCACC CCTCGGTACC TCGTGCTTAT CGCTGAGAGC 
TGTGGCAACC GGGAACTCCT CTGTGGGCTC AGCGACAGAT TCCGGTATTC AGAAGATGAC 
GTGGCCACCT ATGTGGTGCA GCTGCTACAA GGCCTGGACT ACCTCCACGG CCACCACGTG 
CTACACCTGG ACATCAAGCC GGACAATCTA CTGTTGGCCG CTGACAATGC CCTTAAAATT 
GTGGACTTCG GCAGTGCTCA GCCCTATAAC CCTCAGGCCC TGAAGCCACT TGGCCACCGC 
ACAGGCACTC TGGAGTTCAT GGCTCCTGAG ATGGTGAAGG GAGACCCCAT TGGCTCTGCC 
ACGGACATCT GGGGAGCGGG TGTGCTCACC TACATCATGC TTAGTGGGTA CTCCCCATTC 
TATGAGCCAG ACCCCCAGGA AACAGAGGCT CGGATTGTTG GGGGTCGCTT TGATGCCTTC 
CAGTTGTATC CTAACACATC CCAGAGTGCC ACCCTCTTCT TGAGAAAGGT CCTCTCAGTA 
CATCCCTGGA GCCGGCCCTC TCTGCAGGAC TGCTTGGCCC ACCCATGGCT GCAAGATGCC 
TACCTGATGA AGCTGCGCCG CCAGACACTC ACCTTCACCA CCAACCGGCT CAAGGAATTC 
CTGGGCGAGC AGCGGCGACG TCGGGCTGAG GCTGCTACCC GTCACAAGGT GCTGCTCCGC 
TCCTACCCTG GCAGCCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_017322354.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177843.1 Mus musculus SPEG complex locus (Speg), transcript variant X4, mRNA.

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CloneID	OMu76944
Clone ID Related Accession (Same CDS sequence)	XM_017322354.1 , XM_017322354.2
Accession Version	XM_017322354.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017322354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACC AGTGTTGGTA CCTTCTGGGG AGGGGGTCTC CCTCTCCTCC CCATCTCACA 
GAACATCTCT GGTATCCCTG GACAGCAGGG ACCCTCATGC CTCCCTATAT TCTGCCTGCC 
TTCCATGCCC CATCCCACAA ACACTCTGAT AACTGTCTGT CCACATCTCT CTCCTGCTGC 
TCCTATGGAA GCGAAGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA 
GCACAGACAA CCGGGGCCGG GCCACGGCAC CTGGGGGTGG AGCCGCTGGT GCGGGCATCG 
CGAGCTAATC TGGTGGGCGC AAGCTGGGGG TCAGAGGATA GCCTTTCGGT GGCCAGTGAC 
CTCTATGGCA GCGCGTTCAG TCTGTACAGA GGACGGGCGC TCTCGATCCA CGTCAGCATT 
CCTCCGAGTG GATTGCACAG AGAGGAGCCT GACCTTCAGC CTCAGCCAGC CAGTGATGCC 
CTTCGTCCAC GCCCTGCCCT TCCACCTCCC TCCAAATCCG CTTTGCTTCC CCCACCATCT 
CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA CCTAGTACCC AGCCCCCAGC TACTCCCACT 
TCGCCCCATC GGCGTGCTCA GGAGCCCTCG CTGCCTGAGG ACATCACCAC CACGGAAGAG 
AAGCGAGGGA AAAAGCCCAA GTCATCCGGG CCGTCGCTGG CGGGCACAGT GGAGTCCCGG 
CCCCAGACGC CACTGAGCGA GGCTTCGGGT CGCCTGTCAG CATTGGGCCG CTCGCCCCGG 
CTGGTGCGCG CGGGGTCCCG CATCCTGGAC AAGCTACAGT TCTTCGAAGA GCGGCGACGC 
AGCCTGGAGC GCAGCGACTC GCCGCCAGCG CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG 
GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC 
TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG 
CAAAAGGCGG CCTCGTTGGA TGAACGCACG CGACAACGCA GTGCAACCTC GGACCTCGAA 
CTCCGCTTCG CCCAGGAGCT GGGTCGCATC CGCCGATCTA CGTCGCGGGA GGAGCTGGTG 
CGTTCGCACG AGTCCCTGCG TGCCACGCTG CAGCGCGCCC CATCCCCTCG GGAGCCCGGC 
GAGCCCCCAC TCTTCTCCCG GCCTTCCACA CCCAAGACCT CACGGGCTGT GAGCCCGGCT 
GCCACCCAGC CGCCGCCTCC TAGTGGTGCG GGCAAATCTG GGGACGAGCC TGGGAGGCCC 
CGAAGCAGAG GGCCGGTGGG CAGGACTGAA CCGGGGGAAG GCCCGCAGCA GGAGATCAAG 
CGTCGGGACC AATTCCCGCT AACCAGGAGC AGAGCCATCC AGGAGTGCAG GAGCCCTGTG 
CCGCCCTACA CCGCGGATCC CCCGGAGAGC AGGACAAAAG CCCCCTCCGG TCGCAAGCGG 
GAACCCCCTG CTCAAGCGGT GCGCTTTCTG CCCTGGGCCA CTCCGGGAGT GGAGGACTCT 
GTTCTGCCCC AAACCTTGGA GAAGAATAGA GCGGGACCCG AGGCTGAGAA GAGGCTTCGC 
AGAGGACCTG AGGAGGATGG CCCCTGGGGG CCCTGGGACC GCAGAGGGAC CCGCAGCCAA 
GGCAAAGGTC GCCGTGCTCG GCCTACTTCC CCCGAGCTCG AGTCCTCAGA CGACTCCTAT 
GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA CCCGTGTTTG AGATCCCTCT GCAGAATATG 
GTGGTGGCGC CAGGAGCTGA CGTGCTACTT AAGTGTATCA TCACCGCCAA CCCCCCACCC 
CAAGTGTCCT GGAAAAAGGA TGGGTCCATG TTGCACAGCG AGGGTCGTCT TCTCATCCGG 
GCTGAAGGTG AACGGCACAC ACTGCTGCTC AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC 
TACACAGCCA CTGCCACCAA CGAACTGGGC CAAGCTACCT GTGCTTCTTC ACTGGCTGTG 
AGACCTGGCG GCTCCACATC CCCTTTCAGC AGCCCCATCA CCTCTGATGA GGAGTACCTG 
AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG GAGACCTGGC CCCGAACCCC TACCATGAAG 
CTCAGTCCCA GCCAGGATCA TGATTCCTCC GACTCTTCTT CCAAGGCACC CCCAACGTTC 
AAGGTCTCAC TCATGGACCA ATCGGTGAGA GAAGGTCAAG ATGTCATTAT GAGCATCCGT 
GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC TGGCTGAGGA ATCGACAGCC CGTGCGCCCA 
GACCAGCGGC GCTTTGCAGA GGAGGCCGAG GGTGGGCTCT GCCGCTTGAG GATCCTGGCT 
GCTGAACGGG GCGATGCTGG TTTCTACACA TGCAAGGCGG TCAACGAATA TGGCGCTCGG 
CAGTGCGAGG CCCGCCTGGA GGTCCGAGCA CACCCTGAAA GCCGGTCCCT GGCCGTGCTG 
GCCCCCCTGC AGGACGTGGA CGTGGGGGCC GGGGAGATGG CGCTGTTTGA GTGCCTGGTG 
GCAGGTCCAG CTGACGTCGA GGTGGACTGG CTGTGCCGGG GCCGCCTGCT GCAGCCTGCG 
CTGCTCAAAT GCAAGATGCA TTTTGATGGC CGCAAGTGCA AGCTGCTGCT CACCTCTGTG 
CACGAGGACG ACAGTGGTGT CTACACCTGC AAGCTGAGCA CAGCCAAAGA TGAACTGACC 
TGCAGTGCCC GGCTGACGGT TCGGCCGTCG CTGGCGCCTC TCTTCACCCG GTTGCTGGAA 
GATGTGGAGG TGTTGGAAGG CCGTGCTGCC CGCTTGGATT GCAAGATCAG CGGTACCCCG 
CCTCCCTCCG TTACCTGGAC TCATTTTGGC CACCCCGTGA ATGAGGGTGA CAACTTGCGG 
CTTCAACAGG ATGGAGGTCT ACACTCACTG CACATTGCCC GGGTGGGCAG TGAGGACGAA 
GGACTCTATG AAGTCAGTGC CACCAACACT CATGGCCAGG CCCACTGCTC CGCCCAGCTG 
TATGTGGAGG AGCCTCGGAC AGCTGCCTCT GGCCCCAGCT CGAAGCTGGA GAAGATGCCA 
TCTATCCCTG AGGAGCCGGA GCACGGAGAT CTAGAGAGGC TGTCTATTCC TGACTTCCTA 
CGGCCACTGC AAGATCTGGA AGTGGGACTA GCTAAGGAGG CCATGCTGGA GTGCCAAGTG 
ACCGGCCTGC CCTATCCCAC CATCAGCTGG TTCCACAATG GCCACCGCAT TCAAAGCAGT 
GACGACCGAC GCATGACACA GTACAGGGAC ATACATCGCC TGGTGTTCCC AGCTGTGGGG 
CCTCAGCATG CTGGTGTCTA TAAGAGTGTA ATCGCCAACA AGCTGGGCAA AGCGGCCTGC 
TACGCCCATC TCTATGTCAC AGATGTGGTT CCAGGCCCTC CAGATGGTGC TCCGGAAGTA 
GTAGCTGTGA CCGGAAGGAT GGTCACACTA TCGTGGAACC CACCCAGGAG TCTGGACATG 
GCTATTGACC CAGATTCCCT AACATACACT GTGCAGCACC AGGTGTTGGG CTCGGACCAG 
TGGACGGCAC TGGTCACAGG CCTGCGGGAG CCTGCGTGGG CAGCCACAGG GCTGAAGAAG 
GGGATCCAGC ACATCTTCCG GGTCCTCAGC TCCAGTGGCA AGAGCAGCAG CAAGCCCTCA 
GCACCGTCGG AACCCGTGCA GCTGCTAGAG CATGGCCCAC CCCTGGAAGA GGCGCCTGCT 
GTGCTGGACA AGCAGGACAT TGTGTATGTG GTAGAGGGCC AGCCTGCCTG TGTCACCGTC 
ACTTTTAACC ATGTGGAGGC CCAGGTTGTC TGGAGGAGCT GCCGGGGAGC CCTCCTCGAG 
GCGCGCACAG GTGTGTATGA ACTGAGTCAG CCAGATGACG ACCAGTATTG TCTGCGGATC 
TGCCGGGTGA GCCGACGAGA CCTGGGACCC CTTACTTGCA GTGCCCGAAA CCGCCACGGC 
ACAAAGGCCT GCTCGGTCAC CCTGGAGCTG GCAGAGGCAC CTCGGTTTGA GTCTATCATG 
GAAGATGTGG AGGTGGGGCC TGGAGAAACC GCTCGCTTTG CTGTGGTAGT TGAGGGGAAA 
CCACTGCCAG ACATCATGTG GTACAAGGAT GAGGTGCTGC TGGCCGAGAG CAACCATGTG 
AGCTTCGTGT ATGAGGAGAA CGAGTGTTCC CTAGTGCTGC TCAGCGCAGG AAGCCAGGAT 
GGAGGCGTCT ATACCTGTAC CGCCCGGAAC CTGGCAGGCG AAGTCTCCTG CAAGGCGGAG 
CTGTCTGTGC TTTCAGCTCA GACAGCCATG GAGGTCGAGG GGGTCGGAGA GGATGAGGAA 
CACCGAGGAA GAAGACTTAG CGACTACTAC GACATCCACC AAGAGATTGG CAGGGGTGCC 
TTCTCCTACC TGCGGCGTGT AGTGGAGAGG AGCTCTGGCC TGGAGTTTGC AGCCAAGTTC 
ATCCCTAGCC AGGCTAAGCC TAAAGCATCA GCAAGACGCG AGGCGCGCTT GCTGGCCCGG 
CTCCAGCATG GCTGTGTGCT CTACTTCCAC GAAGCCTTTG AGAGGCGCAG GGGACTTGTC 
ATAGTCACGG AGCTCTGCAC AGAAGAGCTT CTAGAGCGCA TGGCCAGGAA GCCCACCGTG 
TGTGAGTCTG AGACTCGGAC CTATATGCGT CAAGTGCTAG AGGGGATATG CTATCTGCAT 
CAGAGCCATG TGCTGCACCT GGATGTCAAG CCGGAGAACC TGCTTGTGTG GGATGGTGCA 
GGGGGTGAAG AGCAGGTGCG CATCTGTGAC TTCGGGAACG CCCAGGAACT CACTCCGGGA 
GAGCCCCAGT ACTGTCAGTA TGGCACGCCA GAGTTTGTAG CACCTGAGAT TGTCAACCAG 
AGCCCTGTGT CTGGAGTCAC TGACATCTGG CCCGTGGGCG TCGTTGCCTT TCTCTGTTTG 
ACGGGCATCT CCCCATTTGT TGGGGAGAAT GACCGGACAA CACTCATGAA TATCCGAAAC 
TACAACGTGG CATTTGAGGA GACTACGTTC CTGAGTCTGA GCAGGGAGGC CCGGGGCTTC 
CTCATCAAAG TGCTGGTGCA GGATCGACTG AGACCTACTG CAGAAGAGAC ATTGGAACAT 
CCTTGGTTCA AAACAGAAGC AAAGGGTGCA GAGGTGAGCA CAGATCACCT GAAGCTCTTC 
CTGTCACGGA GGAGGTGGCA GCGCTCTCAA ATCAGCTACA AGTGCCACCT GGTGCTGCGC 
CCCATTCCGG AACTGCTACG GGCTCCCCCG GAGCGGGTGT GGGTGGCCAT GCCTAGAAGA 
CAACCCCCGA GTGGGGGACT CTCATCCTCT TCAGACTCAG AAGAGGAAGA GTTGGAGGAA 
CTTCCCTCAG TACCCCGCCC CCTGCAGCCT GAGTTCTCAG GTTCTCGGGT ATCCCTCACC 
GACATCCCCA CCGAAGATGA GGCTCTGGGG ACCCCGGAAG CTGGGGCTGC CACCCCCATG 
GACTGGCAAG AGCAGGAAAG GACTCCCTCT AAGGACCAGG AGGCCCCCAG CCCTGAGGCA 
CTCCCCTCTC CAGGCCAGGA GTCCCCTGAT GGGCCTAGCC CCAGGCGGCC AGAGCTCCGC 
AGGGGTAGCT CTGCTGAGAG TGCCCTGCCC CGAGTCGGGT CGCGGGAACC GGGCCGGAGC 
TTGCACAAGG CAGCATCCGT GGAGCTGCCG CAGCGCAGGA GCCCCAGCCC GGGTGCCACC 
CGCCTGACTC GGGGAGGCCT GGGTGAGGGC GAGTATGCCC AGAGGCTGCA GGCCCTGCGT 
CAGCGGCTGC TGAGGGGAGG CCCCGAGGAT GGCAAGGTCA GCGGCCTCAG GGGACCCCTG 
CTGGAGAGCC TGGGGGGCCG TGCACGGGAT CCCAGGATGG CAAGGGCTGC CTCCAGCGAG 
GCTGCCCCCC ACCACCAGCC CCCTCCGGAG TCCCGGGGTC TGCAAAAGAG CAGCAGCTTC 
TCTCAGGGCG AGGCAGAGCC CCGGGGTCGG CACCGCCGCG CGGGGGCACC CCTCGAAATC 
CCCGTGGCCA GACTTGGGGC TCGCAGGCTG CAGGAGTCTC CCTCCTTATC TGCCCTTAGC 
GAGACCCAGC CACCGAGCCC TGCGAGGCCC AGCGTCCCCA AACTCAGTAT CACCAAGTCC 
CCGGAACCTT CCGCTGTCAC ATCCAGAGAC TCTCCCCAGC CCCCGGAACC TCAGCCTGTC 
CCAGAAAAGG TCCCTGAGCC CAAACCAGAA CCAGTCCGAG CTGCAAAACC AGCACAACCC 
CCTTTGGCTC TGCAAATGCC AACGCAGCCT CTCACCCCCT ATGCCCAGAT CATGCAGTCG 
CTCCAGCTGT CCAGTCCCAC CCTGTCCCCA CAGGACCCCG CTGTGCCTCC ATCAGAGCCC 
AAGCCCCACG CGGCTGTGTT TGCCAGGGTG GCTTCCCCAC CACCCGGAGT CTCTGAGAAG 
CGCGTGCCTT CGGCCAGAAC TCCCCCAGTG CTAGCCGAGA AAGCCCGAGT CCCCACGGTG 
CCCCCCAGGC CAGGCAGCAG CCTCAGCGGC AGCATCGAAA ACCTTGAATC AGAGGCTGTG 
TTCGAGGCTA AGTTCAAACG CAGCCGCGAA TCGCCACTGT CGCGGGGCCT GAGGCTACTG 
AGCCGCTCGC GCTCGGAGGA GCGCGGTCCC TTCCGTGGGG CGGAGGACGA CGGCATATAC 
CGGCCCAGTC CGGCAGGAAC GCCGCTAGAG CTGGTGCGAC GGCCCGAGCG CTCGCGCTCC 
GTGCAGGACC TCAGGGTAGC CGGAGAGCCG GGCCTTGTGC GTCGCCTTTC GCTGTCCCTG 
TCGCAGAAGC TGCGGCGGAC ACCGCCGGGA CAGCGACACC CTGCCTGGGA GTCCCGCAGC 
GGAGACGGGG AGAGCTCCGA GGGCGGCAGC TCAGCTCGGG CATCCCCAGT GCTGGCGGTA 
CGCAGGAGAC TCAGCTCCAC GCTGGAGCGT CTGTCCAGCC GTTTGCAGCG CAGCGGCAGC 
AGCGAGGACT CCGGGGGCGC CTCGGGTCGC AGCACGCCGC TGTTTGGACG GCTGCGCAGG 
GCTACTTCGG AAGGGGAGAG CCTGCGACGC CTCGGTGTTC CGCACAATCA GCTGGGCTCT 
CAGACTGGTG CCACTACACC TTCCGCTGAG TCTCTGGGCT CAGAGGCCAG TGGCACATCG 
GGTTCTTCAG CTCCTGGAGA AAGCCGAAGC CGGCACCGGT GGGGCCTTTC CAGGCTGCGC 
AAGGACAAGG GATTATCTCA GCCAAACCTC TCCTCCAGTG TCCAAGAGGA CTTGGGTCAC 
CAGTATGTGC CCAGTGAGTC AGATTTCCCC CCAGTCTTCC ACATCAAACT CAAGGACCAG 
GTGCTGTTGG AGGGGGAAGC AGCCACCCTG CTCTGCCTAC CCGCAGCCTG CCCAGCACCC 
CGCATCTCCT GGATGAAAGA TAAGCAGTCC CTGCGTTCAG AGCCCTCAGT GGTCATTGTG 
TCCTGCAAAG ATGGACGGCA GCTGCTGAGC ATACCCCGGG CCGGCAAGCG GCATGCTGGG 
CTCTATGAGT GCTCAGCCAC CAATGTCCTG GGCAGCATTA CCAGCTCCTG TACTGTGGCC 
GTGGCCCGCA TTCCAGGGAA GCTAGCTCCT CCAGAGGTGC CCCAGACCTA CCATGACACA 
GCACTAGTAG TGTGGAAGCC AGGAGACGGT CGGGCACCTT GCACATACAC TCTGGAGCGG 
CGGGTGGATG GGGAATCTGT CTGGCACCCT GTGAGCTCAG GCATCCCCGA CTGTTATTAC 
AATGTGACTC AGCTGCCTGT CGGCGTGACC GTGCGGTTCC GTGTGGCCTG TTCCAATCGT 
GCTGGGCAGG GGCCCTTCAG CAACCCTTCT GAGAAGGTCT TCATCCGAGG CACTCCAGAT 
TCTCCTGCTC AGCCAGCTGC TGCTCCCCGA GATGCCCCTG TTACCTCAGG GCCAACCAGG 
GCCCCACCGC CTGACTCTCC TACCTCACTG GCCCCCACCC CCGCTCTTGC GCCCCCAGCC 
TCCCAGGCAT CCACTCTCAG CCCCTCAACT TCCTCCATGT CTGCCAACCA GGCCTTGTCC 
TCACTCAAGG CTGTGGGTCC ACCACCCGCA ACCCCTCCAC GAAAGCACAG GGGCCTTCTG 
GCCACCCAGC AAGCAGAGCC ATCCCCACCC AGTATCGTGG TCACCCCAAG TGAACCCAGG 
TCTTTTGTCC CTGACACTGG AACCCTGACC CCAACCTCTA GTCCTCAAGG GGTTAAACCA 
GCACCTTCCT CAACTTCCTT GTATATGGTG ACTTCCTTCG TGTCTGCACC TCCAGCCCCC 
CAGGCCCCAG CCCCTGAGCC CCCTCCTGAG CCCACCAAGG TGACTGTGCG GAGCCTCAGC 
CCAGCCAAGG AGGTGGTCAG CTCCCCAACT CCTGAGAGCA CCACTCTTCG GCAGGGTCCC 
CCTCAGAAAC CCTACACCTT CCTGGAGGAG AAGGCCAGGG GCCGCTTTGG CGTTGTGCGG 
TCATGCCGGG AGAATGCTAC GGGCCGAACG TTTGTCGCCA AGATCGTGCC GTACGCCGCG 
GAGGGCAAGC GACGCGTGCT GCAGGAATAC GAGGTGCTTC GGACGCTGCA CCACGAGCGG 
CTCATGTCCC TGCACGAGGC CTACATCACC CCTCGGTACC TCGTGCTTAT CGCTGAGAGC 
TGTGGCAACC GGGAACTCCT CTGTGGGCTC AGCGACAGAT TCCGGTATTC AGAAGATGAC 
GTGGCCACCT ATGTGGTGCA GCTGCTACAA GGCCTGGACT ACCTCCACGG CCACCACGTG 
CTACACCTGG ACATCAAGCC GGACAATCTA CTGTTGGCCG CTGACAATGC CCTTAAAATT 
GTGGACTTCG GCAGTGCTCA GCCCTATAAC CCTCAGGCCC TGAAGCCACT TGGCCACCGC 
ACAGGCACTC TGGAGTTCAT GGCTCCTGAG ATGGTGAAGG GAGACCCCAT TGGCTCTGCC 
ACGGACATCT GGGGAGCGGG TGTGCTCACC TACATCATGC TTAGTGGGTA CTCCCCATTC 
TATGAGCCAG ACCCCCAGGA AACAGAGGCT CGGATTGTTG GGGGTCGCTT TGATGCCTTC 
CAGTTGTATC CTAACACATC CCAGAGTGCC ACCCTCTTCT TGAGAAAGGT CCTCTCAGTA 
CATCCCTGGA GCCGGCCCTC TCTGCAGGAC TGCTTGGCCC ACCCATGGCT GCAAGATGCC 
TACCTGATGA AGCTGCGCCG CCAGACACTC ACCTTCACCA CCAACCGGCT CAAGGAATTC 
CTGGGCGAGC AGCGGCGACG TCGGGCTGAG GCTGCTACCC GTCACAAGGT GCTGCTCCGC 
TCCTACCCTG GCAGCCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177843.1
CDS	2105..11725
Translation	MAHQCWYLLGRGSPSPPHLTEHLWYPWTAGTLMPPYILPAFHAPSHKHSDNCLSTSLSCCSYGSEVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPRQAQTTGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_017322354.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322354.2

ORF Insert Sequence:

ATGGCTCACC AGTGTTGGTA CCTTCTGGGG AGGGGGTCTC CCTCTCCTCC CCATCTCACA 
GAACATCTCT GGTATCCCTG GACAGCAGGG ACCCTCATGC CTCCCTATAT TCTGCCTGCC 
TTCCATGCCC CATCCCACAA ACACTCTGAT AACTGTCTGT CCACATCTCT CTCCTGCTGC 
TCCTATGGAA GCGAAGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGGCAA 
GCACAGACAA CCGGGGCCGG GCCACGGCAC CTGGGGGTGG AGCCGCTGGT GCGGGCATCG 
CGAGCTAATC TGGTGGGCGC AAGCTGGGGG TCAGAGGATA GCCTTTCGGT GGCCAGTGAC 
CTCTATGGCA GCGCGTTCAG TCTGTACAGA GGACGGGCGC TCTCGATCCA CGTCAGCATT 
CCTCCGAGTG GATTGCACAG AGAGGAGCCT GACCTTCAGC CTCAGCCAGC CAGTGATGCC 
CTTCGTCCAC GCCCTGCCCT TCCACCTCCC TCCAAATCCG CTTTGCTTCC CCCACCATCT 
CCTCGGGTGG GAAAGAGGGC TCTGCCAGGA CCTAGTACCC AGCCCCCAGC TACTCCCACT 
TCGCCCCATC GGCGTGCTCA GGAGCCCTCG CTGCCTGAGG ACATCACCAC CACGGAAGAG 
AAGCGAGGGA AAAAGCCCAA GTCATCCGGG CCGTCGCTGG CGGGCACAGT GGAGTCCCGG 
CCCCAGACGC CACTGAGCGA GGCTTCGGGT CGCCTGTCAG CATTGGGCCG CTCGCCCCGG 
CTGGTGCGCG CGGGGTCCCG CATCCTGGAC AAGCTACAGT TCTTCGAAGA GCGGCGACGC 
AGCCTGGAGC GCAGCGACTC GCCGCCAGCG CCCCTGCGGC CCTGGGTGCC CCTGCGCAAG 
GCTCGCTCGC TGGAGCAGCC GAAGTCCGAG GGCGGTGCGG CGTGGGGCAC ACCCGAGGCC 
TCGCAGGAGG AGCTGCGGTC ACCTCGGGGC AGTGTGGCAG AGCGGCGTCG CCTGTTCCAG 
CAAAAGGCGG CCTCGTTGGA TGAACGCACG CGACAACGCA GTGCAACCTC GGACCTCGAA 
CTCCGCTTCG CCCAGGAGCT GGGTCGCATC CGCCGATCTA CGTCGCGGGA GGAGCTGGTG 
CGTTCGCACG AGTCCCTGCG TGCCACGCTG CAGCGCGCCC CATCCCCTCG GGAGCCCGGC 
GAGCCCCCAC TCTTCTCCCG GCCTTCCACA CCCAAGACCT CACGGGCTGT GAGCCCGGCT 
GCCACCCAGC CGCCGCCTCC TAGTGGTGCG GGCAAATCTG GGGACGAGCC TGGGAGGCCC 
CGAAGCAGAG GGCCGGTGGG CAGGACTGAA CCGGGGGAAG GCCCGCAGCA GGAGATCAAG 
CGTCGGGACC AATTCCCGCT AACCAGGAGC AGAGCCATCC AGGAGTGCAG GAGCCCTGTG 
CCGCCCTACA CCGCGGATCC CCCGGAGAGC AGGACAAAAG CCCCCTCCGG TCGCAAGCGG 
GAACCCCCTG CTCAAGCGGT GCGCTTTCTG CCCTGGGCCA CTCCGGGAGT GGAGGACTCT 
GTTCTGCCCC AAACCTTGGA GAAGAATAGA GCGGGACCCG AGGCTGAGAA GAGGCTTCGC 
AGAGGACCTG AGGAGGATGG CCCCTGGGGG CCCTGGGACC GCAGAGGGAC CCGCAGCCAA 
GGCAAAGGTC GCCGTGCTCG GCCTACTTCC CCCGAGCTCG AGTCCTCAGA CGACTCCTAT 
GTGTCCGCTG GGGAAGAGCC CCTGGAGGCA CCCGTGTTTG AGATCCCTCT GCAGAATATG 
GTGGTGGCGC CAGGAGCTGA CGTGCTACTT AAGTGTATCA TCACCGCCAA CCCCCCACCC 
CAAGTGTCCT GGAAAAAGGA TGGGTCCATG TTGCACAGCG AGGGTCGTCT TCTCATCCGG 
GCTGAAGGTG AACGGCACAC ACTGCTGCTC AGAGAGGCCC AGGCTGCCGA TGCTGGGAGC 
TACACAGCCA CTGCCACCAA CGAACTGGGC CAAGCTACCT GTGCTTCTTC ACTGGCTGTG 
AGACCTGGCG GCTCCACATC CCCTTTCAGC AGCCCCATCA CCTCTGATGA GGAGTACCTG 
AGCCCCCCAG AGGAGTTCCC AGAGCCTGGG GAGACCTGGC CCCGAACCCC TACCATGAAG 
CTCAGTCCCA GCCAGGATCA TGATTCCTCC GACTCTTCTT CCAAGGCACC CCCAACGTTC 
AAGGTCTCAC TCATGGACCA ATCGGTGAGA GAAGGTCAAG ATGTCATTAT GAGCATCCGT 
GTGCAGGGAG AGCCCAAGCC TGTGGTTTCC TGGCTGAGGA ATCGACAGCC CGTGCGCCCA 
GACCAGCGGC GCTTTGCAGA GGAGGCCGAG GGTGGGCTCT GCCGCTTGAG GATCCTGGCT 
GCTGAACGGG GCGATGCTGG TTTCTACACA TGCAAGGCGG TCAACGAATA TGGCGCTCGG 
CAGTGCGAGG CCCGCCTGGA GGTCCGAGCA CACCCTGAAA GCCGGTCCCT GGCCGTGCTG 
GCCCCCCTGC AGGACGTGGA CGTGGGGGCC GGGGAGATGG CGCTGTTTGA GTGCCTGGTG 
GCAGGTCCAG CTGACGTCGA GGTGGACTGG CTGTGCCGGG GCCGCCTGCT GCAGCCTGCG 
CTGCTCAAAT GCAAGATGCA TTTTGATGGC CGCAAGTGCA AGCTGCTGCT CACCTCTGTG 
CACGAGGACG ACAGTGGTGT CTACACCTGC AAGCTGAGCA CAGCCAAAGA TGAACTGACC 
TGCAGTGCCC GGCTGACGGT TCGGCCGTCG CTGGCGCCTC TCTTCACCCG GTTGCTGGAA 
GATGTGGAGG TGTTGGAAGG CCGTGCTGCC CGCTTGGATT GCAAGATCAG CGGTACCCCG 
CCTCCCTCCG TTACCTGGAC TCATTTTGGC CACCCCGTGA ATGAGGGTGA CAACTTGCGG 
CTTCAACAGG ATGGAGGTCT ACACTCACTG CACATTGCCC GGGTGGGCAG TGAGGACGAA 
GGACTCTATG AAGTCAGTGC CACCAACACT CATGGCCAGG CCCACTGCTC CGCCCAGCTG 
TATGTGGAGG AGCCTCGGAC AGCTGCCTCT GGCCCCAGCT CGAAGCTGGA GAAGATGCCA 
TCTATCCCTG AGGAGCCGGA GCACGGAGAT CTAGAGAGGC TGTCTATTCC TGACTTCCTA 
CGGCCACTGC AAGATCTGGA AGTGGGACTA GCTAAGGAGG CCATGCTGGA GTGCCAAGTG 
ACCGGCCTGC CCTATCCCAC CATCAGCTGG TTCCACAATG GCCACCGCAT TCAAAGCAGT 
GACGACCGAC GCATGACACA GTACAGGGAC ATACATCGCC TGGTGTTCCC AGCTGTGGGG 
CCTCAGCATG CTGGTGTCTA TAAGAGTGTA ATCGCCAACA AGCTGGGCAA AGCGGCCTGC 
TACGCCCATC TCTATGTCAC AGATGTGGTT CCAGGCCCTC CAGATGGTGC TCCGGAAGTA 
GTAGCTGTGA CCGGAAGGAT GGTCACACTA TCGTGGAACC CACCCAGGAG TCTGGACATG 
GCTATTGACC CAGATTCCCT AACATACACT GTGCAGCACC AGGTGTTGGG CTCGGACCAG 
TGGACGGCAC TGGTCACAGG CCTGCGGGAG CCTGCGTGGG CAGCCACAGG GCTGAAGAAG 
GGGATCCAGC ACATCTTCCG GGTCCTCAGC TCCAGTGGCA AGAGCAGCAG CAAGCCCTCA 
GCACCGTCGG AACCCGTGCA GCTGCTAGAG CATGGCCCAC CCCTGGAAGA GGCGCCTGCT 
GTGCTGGACA AGCAGGACAT TGTGTATGTG GTAGAGGGCC AGCCTGCCTG TGTCACCGTC 
ACTTTTAACC ATGTGGAGGC CCAGGTTGTC TGGAGGAGCT GCCGGGGAGC CCTCCTCGAG 
GCGCGCACAG GTGTGTATGA ACTGAGTCAG CCAGATGACG ACCAGTATTG TCTGCGGATC 
TGCCGGGTGA GCCGACGAGA CCTGGGACCC CTTACTTGCA GTGCCCGAAA CCGCCACGGC 
ACAAAGGCCT GCTCGGTCAC CCTGGAGCTG GCAGAGGCAC CTCGGTTTGA GTCTATCATG 
GAAGATGTGG AGGTGGGGCC TGGAGAAACC GCTCGCTTTG CTGTGGTAGT TGAGGGGAAA 
CCACTGCCAG ACATCATGTG GTACAAGGAT GAGGTGCTGC TGGCCGAGAG CAACCATGTG 
AGCTTCGTGT ATGAGGAGAA CGAGTGTTCC CTAGTGCTGC TCAGCGCAGG AAGCCAGGAT 
GGAGGCGTCT ATACCTGTAC CGCCCGGAAC CTGGCAGGCG AAGTCTCCTG CAAGGCGGAG 
CTGTCTGTGC TTTCAGCTCA GACAGCCATG GAGGTCGAGG GGGTCGGAGA GGATGAGGAA 
CACCGAGGAA GAAGACTTAG CGACTACTAC GACATCCACC AAGAGATTGG CAGGGGTGCC 
TTCTCCTACC TGCGGCGTGT AGTGGAGAGG AGCTCTGGCC TGGAGTTTGC AGCCAAGTTC 
ATCCCTAGCC AGGCTAAGCC TAAAGCATCA GCAAGACGCG AGGCGCGCTT GCTGGCCCGG 
CTCCAGCATG GCTGTGTGCT CTACTTCCAC GAAGCCTTTG AGAGGCGCAG GGGACTTGTC 
ATAGTCACGG AGCTCTGCAC AGAAGAGCTT CTAGAGCGCA TGGCCAGGAA GCCCACCGTG 
TGTGAGTCTG AGACTCGGAC CTATATGCGT CAAGTGCTAG AGGGGATATG CTATCTGCAT 
CAGAGCCATG TGCTGCACCT GGATGTCAAG CCGGAGAACC TGCTTGTGTG GGATGGTGCA 
GGGGGTGAAG AGCAGGTGCG CATCTGTGAC TTCGGGAACG CCCAGGAACT CACTCCGGGA 
GAGCCCCAGT ACTGTCAGTA TGGCACGCCA GAGTTTGTAG CACCTGAGAT TGTCAACCAG 
AGCCCTGTGT CTGGAGTCAC TGACATCTGG CCCGTGGGCG TCGTTGCCTT TCTCTGTTTG 
ACGGGCATCT CCCCATTTGT TGGGGAGAAT GACCGGACAA CACTCATGAA TATCCGAAAC 
TACAACGTGG CATTTGAGGA GACTACGTTC CTGAGTCTGA GCAGGGAGGC CCGGGGCTTC 
CTCATCAAAG TGCTGGTGCA GGATCGACTG AGACCTACTG CAGAAGAGAC ATTGGAACAT 
CCTTGGTTCA AAACAGAAGC AAAGGGTGCA GAGGTGAGCA CAGATCACCT GAAGCTCTTC 
CTGTCACGGA GGAGGTGGCA GCGCTCTCAA ATCAGCTACA AGTGCCACCT GGTGCTGCGC 
CCCATTCCGG AACTGCTACG GGCTCCCCCG GAGCGGGTGT GGGTGGCCAT GCCTAGAAGA 
CAACCCCCGA GTGGGGGACT CTCATCCTCT TCAGACTCAG AAGAGGAAGA GTTGGAGGAA 
CTTCCCTCAG TACCCCGCCC CCTGCAGCCT GAGTTCTCAG GTTCTCGGGT ATCCCTCACC 
GACATCCCCA CCGAAGATGA GGCTCTGGGG ACCCCGGAAG CTGGGGCTGC CACCCCCATG 
GACTGGCAAG AGCAGGAAAG GACTCCCTCT AAGGACCAGG AGGCCCCCAG CCCTGAGGCA 
CTCCCCTCTC CAGGCCAGGA GTCCCCTGAT GGGCCTAGCC CCAGGCGGCC AGAGCTCCGC 
AGGGGTAGCT CTGCTGAGAG TGCCCTGCCC CGAGTCGGGT CGCGGGAACC GGGCCGGAGC 
TTGCACAAGG CAGCATCCGT GGAGCTGCCG CAGCGCAGGA GCCCCAGCCC GGGTGCCACC 
CGCCTGACTC GGGGAGGCCT GGGTGAGGGC GAGTATGCCC AGAGGCTGCA GGCCCTGCGT 
CAGCGGCTGC TGAGGGGAGG CCCCGAGGAT GGCAAGGTCA GCGGCCTCAG GGGACCCCTG 
CTGGAGAGCC TGGGGGGCCG TGCACGGGAT CCCAGGATGG CAAGGGCTGC CTCCAGCGAG 
GCTGCCCCCC ACCACCAGCC CCCTCCGGAG TCCCGGGGTC TGCAAAAGAG CAGCAGCTTC 
TCTCAGGGCG AGGCAGAGCC CCGGGGTCGG CACCGCCGCG CGGGGGCACC CCTCGAAATC 
CCCGTGGCCA GACTTGGGGC TCGCAGGCTG CAGGAGTCTC CCTCCTTATC TGCCCTTAGC 
GAGACCCAGC CACCGAGCCC TGCGAGGCCC AGCGTCCCCA AACTCAGTAT CACCAAGTCC 
CCGGAACCTT CCGCTGTCAC ATCCAGAGAC TCTCCCCAGC CCCCGGAACC TCAGCCTGTC 
CCAGAAAAGG TCCCTGAGCC CAAACCAGAA CCAGTCCGAG CTGCAAAACC AGCACAACCC 
CCTTTGGCTC TGCAAATGCC AACGCAGCCT CTCACCCCCT ATGCCCAGAT CATGCAGTCG 
CTCCAGCTGT CCAGTCCCAC CCTGTCCCCA CAGGACCCCG CTGTGCCTCC ATCAGAGCCC 
AAGCCCCACG CGGCTGTGTT TGCCAGGGTG GCTTCCCCAC CACCCGGAGT CTCTGAGAAG 
CGCGTGCCTT CGGCCAGAAC TCCCCCAGTG CTAGCCGAGA AAGCCCGAGT CCCCACGGTG 
CCCCCCAGGC CAGGCAGCAG CCTCAGCGGC AGCATCGAAA ACCTTGAATC AGAGGCTGTG 
TTCGAGGCTA AGTTCAAACG CAGCCGCGAA TCGCCACTGT CGCGGGGCCT GAGGCTACTG 
AGCCGCTCGC GCTCGGAGGA GCGCGGTCCC TTCCGTGGGG CGGAGGACGA CGGCATATAC 
CGGCCCAGTC CGGCAGGAAC GCCGCTAGAG CTGGTGCGAC GGCCCGAGCG CTCGCGCTCC 
GTGCAGGACC TCAGGGTAGC CGGAGAGCCG GGCCTTGTGC GTCGCCTTTC GCTGTCCCTG 
TCGCAGAAGC TGCGGCGGAC ACCGCCGGGA CAGCGACACC CTGCCTGGGA GTCCCGCAGC 
GGAGACGGGG AGAGCTCCGA GGGCGGCAGC TCAGCTCGGG CATCCCCAGT GCTGGCGGTA 
CGCAGGAGAC TCAGCTCCAC GCTGGAGCGT CTGTCCAGCC GTTTGCAGCG CAGCGGCAGC 
AGCGAGGACT CCGGGGGCGC CTCGGGTCGC AGCACGCCGC TGTTTGGACG GCTGCGCAGG 
GCTACTTCGG AAGGGGAGAG CCTGCGACGC CTCGGTGTTC CGCACAATCA GCTGGGCTCT 
CAGACTGGTG CCACTACACC TTCCGCTGAG TCTCTGGGCT CAGAGGCCAG TGGCACATCG 
GGTTCTTCAG CTCCTGGAGA AAGCCGAAGC CGGCACCGGT GGGGCCTTTC CAGGCTGCGC 
AAGGACAAGG GATTATCTCA GCCAAACCTC TCCTCCAGTG TCCAAGAGGA CTTGGGTCAC 
CAGTATGTGC CCAGTGAGTC AGATTTCCCC CCAGTCTTCC ACATCAAACT CAAGGACCAG 
GTGCTGTTGG AGGGGGAAGC AGCCACCCTG CTCTGCCTAC CCGCAGCCTG CCCAGCACCC 
CGCATCTCCT GGATGAAAGA TAAGCAGTCC CTGCGTTCAG AGCCCTCAGT GGTCATTGTG 
TCCTGCAAAG ATGGACGGCA GCTGCTGAGC ATACCCCGGG CCGGCAAGCG GCATGCTGGG 
CTCTATGAGT GCTCAGCCAC CAATGTCCTG GGCAGCATTA CCAGCTCCTG TACTGTGGCC 
GTGGCCCGCA TTCCAGGGAA GCTAGCTCCT CCAGAGGTGC CCCAGACCTA CCATGACACA 
GCACTAGTAG TGTGGAAGCC AGGAGACGGT CGGGCACCTT GCACATACAC TCTGGAGCGG 
CGGGTGGATG GGGAATCTGT CTGGCACCCT GTGAGCTCAG GCATCCCCGA CTGTTATTAC 
AATGTGACTC AGCTGCCTGT CGGCGTGACC GTGCGGTTCC GTGTGGCCTG TTCCAATCGT 
GCTGGGCAGG GGCCCTTCAG CAACCCTTCT GAGAAGGTCT TCATCCGAGG CACTCCAGAT 
TCTCCTGCTC AGCCAGCTGC TGCTCCCCGA GATGCCCCTG TTACCTCAGG GCCAACCAGG 
GCCCCACCGC CTGACTCTCC TACCTCACTG GCCCCCACCC CCGCTCTTGC GCCCCCAGCC 
TCCCAGGCAT CCACTCTCAG CCCCTCAACT TCCTCCATGT CTGCCAACCA GGCCTTGTCC 
TCACTCAAGG CTGTGGGTCC ACCACCCGCA ACCCCTCCAC GAAAGCACAG GGGCCTTCTG 
GCCACCCAGC AAGCAGAGCC ATCCCCACCC AGTATCGTGG TCACCCCAAG TGAACCCAGG 
TCTTTTGTCC CTGACACTGG AACCCTGACC CCAACCTCTA GTCCTCAAGG GGTTAAACCA 
GCACCTTCCT CAACTTCCTT GTATATGGTG ACTTCCTTCG TGTCTGCACC TCCAGCCCCC 
CAGGCCCCAG CCCCTGAGCC CCCTCCTGAG CCCACCAAGG TGACTGTGCG GAGCCTCAGC 
CCAGCCAAGG AGGTGGTCAG CTCCCCAACT CCTGAGAGCA CCACTCTTCG GCAGGGTCCC 
CCTCAGAAAC CCTACACCTT CCTGGAGGAG AAGGCCAGGG GCCGCTTTGG CGTTGTGCGG 
TCATGCCGGG AGAATGCTAC GGGCCGAACG TTTGTCGCCA AGATCGTGCC GTACGCCGCG 
GAGGGCAAGC GACGCGTGCT GCAGGAATAC GAGGTGCTTC GGACGCTGCA CCACGAGCGG 
CTCATGTCCC TGCACGAGGC CTACATCACC CCTCGGTACC TCGTGCTTAT CGCTGAGAGC 
TGTGGCAACC GGGAACTCCT CTGTGGGCTC AGCGACAGAT TCCGGTATTC AGAAGATGAC 
GTGGCCACCT ATGTGGTGCA GCTGCTACAA GGCCTGGACT ACCTCCACGG CCACCACGTG 
CTACACCTGG ACATCAAGCC GGACAATCTA CTGTTGGCCG CTGACAATGC CCTTAAAATT 
GTGGACTTCG GCAGTGCTCA GCCCTATAAC CCTCAGGCCC TGAAGCCACT TGGCCACCGC 
ACAGGCACTC TGGAGTTCAT GGCTCCTGAG ATGGTGAAGG GAGACCCCAT TGGCTCTGCC 
ACGGACATCT GGGGAGCGGG TGTGCTCACC TACATCATGC TTAGTGGGTA CTCCCCATTC 
TATGAGCCAG ACCCCCAGGA AACAGAGGCT CGGATTGTTG GGGGTCGCTT TGATGCCTTC 
CAGTTGTATC CTAACACATC CCAGAGTGCC ACCCTCTTCT TGAGAAAGGT CCTCTCAGTA 
CATCCCTGGA GCCGGCCCTC TCTGCAGGAC TGCTTGGCCC ACCCATGGCT GCAAGATGCC 
TACCTGATGA AGCTGCGCCG CCAGACACTC ACCTTCACCA CCAACCGGCT CAAGGAATTC 
CTGGGCGAGC AGCGGCGACG TCGGGCTGAG GCTGCTACCC GTCACAAGGT GCTGCTCCGC 
TCCTACCCTG GCAGCCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_017322375.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177864.1 Mus musculus SPEG complex locus (Speg), transcript variant X6, mRNA.

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CloneID	OMu76945
Clone ID Related Accession (Same CDS sequence)	XM_017322375.1 , XM_017322375.2
Accession Version	XM_017322375.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACC AGTGTTGGTA CCTTCTGGGG AGGGGGTCTC CCTCTCCTCC CCATCTCACA 
GAACATCTCT GGTATCCCTG GACAGCAGGG ACCCTCATGC CTCCCTATAT TCTGCCTGCC 
TTCCATGCCC CATCCCACAA ACACTCTGAT AACTGTCTGT CCACATCTCT CTCCTGCTGC 
TCCTATGGAA GCGAAGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGCAGC 
ATTCCTCCGA GTGGATTGCA CAGAGAGGAG CCTGACCTTC AGCCTCAGCC AGCCAGTGAT 
GCCCTTCGTC CACGCCCTGC CCTTCCACCT CCCTCCAAAT CCGCTTTGCT TCCCCCACCA 
TCTCCTCGGG TGGGAAAGAG GGCTCTGCCA GGACCTAGTA CCCAGCCCCC AGCTACTCCC 
ACTTCGCCCC ATCGGCGTGC TCAGGAGCCC TCGCTGCCTG AGGACATCAC CACCACGGAA 
GAGAAGCGAG GGAAAAAGCC CAAGTCATCC GGGCCGTCGC TGGCGGGCAC AGTGGAGTCC 
CGGCCCCAGA CGCCACTGAG CGAGGCTTCG GGTCGCCTGT CAGCATTGGG CCGCTCGCCC 
CGGCTGGTGC GCGCGGGGTC CCGCATCCTG GACAAGCTAC AGTTCTTCGA AGAGCGGCGA 
CGCAGCCTGG AGCGCAGCGA CTCGCCGCCA GCGCCCCTGC GGCCCTGGGT GCCCCTGCGC 
AAGGCTCGCT CGCTGGAGCA GCCGAAGTCC GAGGGCGGTG CGGCGTGGGG CACACCCGAG 
GCCTCGCAGG AGGAGCTGCG GTCACCTCGG GGCAGTGTGG CAGAGCGGCG TCGCCTGTTC 
CAGCAAAAGG CGGCCTCGTT GGATGAACGC ACGCGACAAC GCAGTGCAAC CTCGGACCTC 
GAACTCCGCT TCGCCCAGGA GCTGGGTCGC ATCCGCCGAT CTACGTCGCG GGAGGAGCTG 
GTGCGTTCGC ACGAGTCCCT GCGTGCCACG CTGCAGCGCG CCCCATCCCC TCGGGAGCCC 
GGCGAGCCCC CACTCTTCTC CCGGCCTTCC ACACCCAAGA CCTCACGGGC TGTGAGCCCG 
GCTGCCACCC AGCCGCCGCC TCCTAGTGGT GCGGGCAAAT CTGGGGACGA GCCTGGGAGG 
CCCCGAAGCA GAGGGCCGGT GGGCAGGACT GAACCGGGGG AAGGCCCGCA GCAGGAGATC 
AAGCGTCGGG ACCAATTCCC GCTAACCAGG AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT 
GTGCCGCCCT ACACCGCGGA TCCCCCGGAG AGCAGGACAA AAGCCCCCTC CGGTCGCAAG 
CGGGAACCCC CTGCTCAAGC GGTGCGCTTT CTGCCCTGGG CCACTCCGGG AGTGGAGGAC 
TCTGTTCTGC CCCAAACCTT GGAGAAGAAT AGAGCGGGAC CCGAGGCTGA GAAGAGGCTT 
CGCAGAGGAC CTGAGGAGGA TGGCCCCTGG GGGCCCTGGG ACCGCAGAGG GACCCGCAGC 
CAAGGCAAAG GTCGCCGTGC TCGGCCTACT TCCCCCGAGC TCGAGTCCTC AGACGACTCC 
TATGTGTCCG CTGGGGAAGA GCCCCTGGAG GCACCCGTGT TTGAGATCCC TCTGCAGAAT 
ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177864.1
CDS	764..10207
Translation	MAHQCWYLLGRGSPSPPHLTEHLWYPWTAGTLMPPYILPAFHAPSHKHSDNCLSTSLSCCSYGSEVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPSSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_017322375.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322375.1

ORF Insert Sequence:

ATGGCTCACC AGTGTTGGTA CCTTCTGGGG AGGGGGTCTC CCTCTCCTCC CCATCTCACA 
GAACATCTCT GGTATCCCTG GACAGCAGGG ACCCTCATGC CTCCCTATAT TCTGCCTGCC 
TTCCATGCCC CATCCCACAA ACACTCTGAT AACTGTCTGT CCACATCTCT CTCCTGCTGC 
TCCTATGGAA GCGAAGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGCAGC 
ATTCCTCCGA GTGGATTGCA CAGAGAGGAG CCTGACCTTC AGCCTCAGCC AGCCAGTGAT 
GCCCTTCGTC CACGCCCTGC CCTTCCACCT CCCTCCAAAT CCGCTTTGCT TCCCCCACCA 
TCTCCTCGGG TGGGAAAGAG GGCTCTGCCA GGACCTAGTA CCCAGCCCCC AGCTACTCCC 
ACTTCGCCCC ATCGGCGTGC TCAGGAGCCC TCGCTGCCTG AGGACATCAC CACCACGGAA 
GAGAAGCGAG GGAAAAAGCC CAAGTCATCC GGGCCGTCGC TGGCGGGCAC AGTGGAGTCC 
CGGCCCCAGA CGCCACTGAG CGAGGCTTCG GGTCGCCTGT CAGCATTGGG CCGCTCGCCC 
CGGCTGGTGC GCGCGGGGTC CCGCATCCTG GACAAGCTAC AGTTCTTCGA AGAGCGGCGA 
CGCAGCCTGG AGCGCAGCGA CTCGCCGCCA GCGCCCCTGC GGCCCTGGGT GCCCCTGCGC 
AAGGCTCGCT CGCTGGAGCA GCCGAAGTCC GAGGGCGGTG CGGCGTGGGG CACACCCGAG 
GCCTCGCAGG AGGAGCTGCG GTCACCTCGG GGCAGTGTGG CAGAGCGGCG TCGCCTGTTC 
CAGCAAAAGG CGGCCTCGTT GGATGAACGC ACGCGACAAC GCAGTGCAAC CTCGGACCTC 
GAACTCCGCT TCGCCCAGGA GCTGGGTCGC ATCCGCCGAT CTACGTCGCG GGAGGAGCTG 
GTGCGTTCGC ACGAGTCCCT GCGTGCCACG CTGCAGCGCG CCCCATCCCC TCGGGAGCCC 
GGCGAGCCCC CACTCTTCTC CCGGCCTTCC ACACCCAAGA CCTCACGGGC TGTGAGCCCG 
GCTGCCACCC AGCCGCCGCC TCCTAGTGGT GCGGGCAAAT CTGGGGACGA GCCTGGGAGG 
CCCCGAAGCA GAGGGCCGGT GGGCAGGACT GAACCGGGGG AAGGCCCGCA GCAGGAGATC 
AAGCGTCGGG ACCAATTCCC GCTAACCAGG AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT 
GTGCCGCCCT ACACCGCGGA TCCCCCGGAG AGCAGGACAA AAGCCCCCTC CGGTCGCAAG 
CGGGAACCCC CTGCTCAAGC GGTGCGCTTT CTGCCCTGGG CCACTCCGGG AGTGGAGGAC 
TCTGTTCTGC CCCAAACCTT GGAGAAGAAT AGAGCGGGAC CCGAGGCTGA GAAGAGGCTT 
CGCAGAGGAC CTGAGGAGGA TGGCCCCTGG GGGCCCTGGG ACCGCAGAGG GACCCGCAGC 
CAAGGCAAAG GTCGCCGTGC TCGGCCTACT TCCCCCGAGC TCGAGTCCTC AGACGACTCC 
TATGTGTCCG CTGGGGAAGA GCCCCTGGAG GCACCCGTGT TTGAGATCCC TCTGCAGAAT 
ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_017322375.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177864.1 Mus musculus SPEG complex locus (Speg), transcript variant X7, mRNA.

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CloneID	OMu76945
Clone ID Related Accession (Same CDS sequence)	XM_017322375.1 , XM_017322375.2
Accession Version	XM_017322375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017322375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACC AGTGTTGGTA CCTTCTGGGG AGGGGGTCTC CCTCTCCTCC CCATCTCACA 
GAACATCTCT GGTATCCCTG GACAGCAGGG ACCCTCATGC CTCCCTATAT TCTGCCTGCC 
TTCCATGCCC CATCCCACAA ACACTCTGAT AACTGTCTGT CCACATCTCT CTCCTGCTGC 
TCCTATGGAA GCGAAGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGCAGC 
ATTCCTCCGA GTGGATTGCA CAGAGAGGAG CCTGACCTTC AGCCTCAGCC AGCCAGTGAT 
GCCCTTCGTC CACGCCCTGC CCTTCCACCT CCCTCCAAAT CCGCTTTGCT TCCCCCACCA 
TCTCCTCGGG TGGGAAAGAG GGCTCTGCCA GGACCTAGTA CCCAGCCCCC AGCTACTCCC 
ACTTCGCCCC ATCGGCGTGC TCAGGAGCCC TCGCTGCCTG AGGACATCAC CACCACGGAA 
GAGAAGCGAG GGAAAAAGCC CAAGTCATCC GGGCCGTCGC TGGCGGGCAC AGTGGAGTCC 
CGGCCCCAGA CGCCACTGAG CGAGGCTTCG GGTCGCCTGT CAGCATTGGG CCGCTCGCCC 
CGGCTGGTGC GCGCGGGGTC CCGCATCCTG GACAAGCTAC AGTTCTTCGA AGAGCGGCGA 
CGCAGCCTGG AGCGCAGCGA CTCGCCGCCA GCGCCCCTGC GGCCCTGGGT GCCCCTGCGC 
AAGGCTCGCT CGCTGGAGCA GCCGAAGTCC GAGGGCGGTG CGGCGTGGGG CACACCCGAG 
GCCTCGCAGG AGGAGCTGCG GTCACCTCGG GGCAGTGTGG CAGAGCGGCG TCGCCTGTTC 
CAGCAAAAGG CGGCCTCGTT GGATGAACGC ACGCGACAAC GCAGTGCAAC CTCGGACCTC 
GAACTCCGCT TCGCCCAGGA GCTGGGTCGC ATCCGCCGAT CTACGTCGCG GGAGGAGCTG 
GTGCGTTCGC ACGAGTCCCT GCGTGCCACG CTGCAGCGCG CCCCATCCCC TCGGGAGCCC 
GGCGAGCCCC CACTCTTCTC CCGGCCTTCC ACACCCAAGA CCTCACGGGC TGTGAGCCCG 
GCTGCCACCC AGCCGCCGCC TCCTAGTGGT GCGGGCAAAT CTGGGGACGA GCCTGGGAGG 
CCCCGAAGCA GAGGGCCGGT GGGCAGGACT GAACCGGGGG AAGGCCCGCA GCAGGAGATC 
AAGCGTCGGG ACCAATTCCC GCTAACCAGG AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT 
GTGCCGCCCT ACACCGCGGA TCCCCCGGAG AGCAGGACAA AAGCCCCCTC CGGTCGCAAG 
CGGGAACCCC CTGCTCAAGC GGTGCGCTTT CTGCCCTGGG CCACTCCGGG AGTGGAGGAC 
TCTGTTCTGC CCCAAACCTT GGAGAAGAAT AGAGCGGGAC CCGAGGCTGA GAAGAGGCTT 
CGCAGAGGAC CTGAGGAGGA TGGCCCCTGG GGGCCCTGGG ACCGCAGAGG GACCCGCAGC 
CAAGGCAAAG GTCGCCGTGC TCGGCCTACT TCCCCCGAGC TCGAGTCCTC AGACGACTCC 
TATGTGTCCG CTGGGGAAGA GCCCCTGGAG GCACCCGTGT TTGAGATCCC TCTGCAGAAT 
ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177864.1
CDS	2105..11548
Translation	MAHQCWYLLGRGSPSPPHLTEHLWYPWTAGTLMPPYILPAFHAPSHKHSDNCLSTSLSCCSYGSEVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPSSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP

Target ORF information:

RefSeq Version	XM_017322375.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322375.2

ORF Insert Sequence:

ATGGCTCACC AGTGTTGGTA CCTTCTGGGG AGGGGGTCTC CCTCTCCTCC CCATCTCACA 
GAACATCTCT GGTATCCCTG GACAGCAGGG ACCCTCATGC CTCCCTATAT TCTGCCTGCC 
TTCCATGCCC CATCCCACAA ACACTCTGAT AACTGTCTGT CCACATCTCT CTCCTGCTGC 
TCCTATGGAA GCGAAGTTTT CCGCTCCTGC AGAAAGCAAA GTTACGACTC GGAGACTGCC 
GAGGATGACA TCAGCGATGT GCCGGGGACC CAGCGCCTGG AGCTTCGGGA TGACAGGGCC 
TTCAGCACCC CCACGGGGGG CTCTGATACC CTGGTGGGTA CCTCCCTGGA CACACCCCCG 
ACCTCCGTGA CAGGCACCTC AGAGGAGCAA GTGAGCTGGT GGGGCAGCGG GCAGACGGTC 
CTGGAGCAGG AAGCGGGCAG CGGGGGTGGC ACCCGCCCCC TCCCAGGCAG CCCAAGCAGC 
ATTCCTCCGA GTGGATTGCA CAGAGAGGAG CCTGACCTTC AGCCTCAGCC AGCCAGTGAT 
GCCCTTCGTC CACGCCCTGC CCTTCCACCT CCCTCCAAAT CCGCTTTGCT TCCCCCACCA 
TCTCCTCGGG TGGGAAAGAG GGCTCTGCCA GGACCTAGTA CCCAGCCCCC AGCTACTCCC 
ACTTCGCCCC ATCGGCGTGC TCAGGAGCCC TCGCTGCCTG AGGACATCAC CACCACGGAA 
GAGAAGCGAG GGAAAAAGCC CAAGTCATCC GGGCCGTCGC TGGCGGGCAC AGTGGAGTCC 
CGGCCCCAGA CGCCACTGAG CGAGGCTTCG GGTCGCCTGT CAGCATTGGG CCGCTCGCCC 
CGGCTGGTGC GCGCGGGGTC CCGCATCCTG GACAAGCTAC AGTTCTTCGA AGAGCGGCGA 
CGCAGCCTGG AGCGCAGCGA CTCGCCGCCA GCGCCCCTGC GGCCCTGGGT GCCCCTGCGC 
AAGGCTCGCT CGCTGGAGCA GCCGAAGTCC GAGGGCGGTG CGGCGTGGGG CACACCCGAG 
GCCTCGCAGG AGGAGCTGCG GTCACCTCGG GGCAGTGTGG CAGAGCGGCG TCGCCTGTTC 
CAGCAAAAGG CGGCCTCGTT GGATGAACGC ACGCGACAAC GCAGTGCAAC CTCGGACCTC 
GAACTCCGCT TCGCCCAGGA GCTGGGTCGC ATCCGCCGAT CTACGTCGCG GGAGGAGCTG 
GTGCGTTCGC ACGAGTCCCT GCGTGCCACG CTGCAGCGCG CCCCATCCCC TCGGGAGCCC 
GGCGAGCCCC CACTCTTCTC CCGGCCTTCC ACACCCAAGA CCTCACGGGC TGTGAGCCCG 
GCTGCCACCC AGCCGCCGCC TCCTAGTGGT GCGGGCAAAT CTGGGGACGA GCCTGGGAGG 
CCCCGAAGCA GAGGGCCGGT GGGCAGGACT GAACCGGGGG AAGGCCCGCA GCAGGAGATC 
AAGCGTCGGG ACCAATTCCC GCTAACCAGG AGCAGAGCCA TCCAGGAGTG CAGGAGCCCT 
GTGCCGCCCT ACACCGCGGA TCCCCCGGAG AGCAGGACAA AAGCCCCCTC CGGTCGCAAG 
CGGGAACCCC CTGCTCAAGC GGTGCGCTTT CTGCCCTGGG CCACTCCGGG AGTGGAGGAC 
TCTGTTCTGC CCCAAACCTT GGAGAAGAAT AGAGCGGGAC CCGAGGCTGA GAAGAGGCTT 
CGCAGAGGAC CTGAGGAGGA TGGCCCCTGG GGGCCCTGGG ACCGCAGAGG GACCCGCAGC 
CAAGGCAAAG GTCGCCGTGC TCGGCCTACT TCCCCCGAGC TCGAGTCCTC AGACGACTCC 
TATGTGTCCG CTGGGGAAGA GCCCCTGGAG GCACCCGTGT TTGAGATCCC TCTGCAGAAT 
ATGGTGGTGG CGCCAGGAGC TGACGTGCTA CTTAAGTGTA TCATCACCGC CAACCCCCCA 
CCCCAAGTGT CCTGGAAAAA GGATGGGTCC ATGTTGCACA GCGAGGGTCG TCTTCTCATC 
CGGGCTGAAG GTGAACGGCA CACACTGCTG CTCAGAGAGG CCCAGGCTGC CGATGCTGGG 
AGCTACACAG CCACTGCCAC CAACGAACTG GGCCAAGCTA CCTGTGCTTC TTCACTGGCT 
GTGAGACCTG GCGGCTCCAC ATCCCCTTTC AGCAGCCCCA TCACCTCTGA TGAGGAGTAC 
CTGAGCCCCC CAGAGGAGTT CCCAGAGCCT GGGGAGACCT GGCCCCGAAC CCCTACCATG 
AAGCTCAGTC CCAGCCAGGA TCATGATTCC TCCGACTCTT CTTCCAAGGC ACCCCCAACG 
TTCAAGGTCT CACTCATGGA CCAATCGGTG AGAGAAGGTC AAGATGTCAT TATGAGCATC 
CGTGTGCAGG GAGAGCCCAA GCCTGTGGTT TCCTGGCTGA GGAATCGACA GCCCGTGCGC 
CCAGACCAGC GGCGCTTTGC AGAGGAGGCC GAGGGTGGGC TCTGCCGCTT GAGGATCCTG 
GCTGCTGAAC GGGGCGATGC TGGTTTCTAC ACATGCAAGG CGGTCAACGA ATATGGCGCT 
CGGCAGTGCG AGGCCCGCCT GGAGGTCCGA GCACACCCTG AAAGCCGGTC CCTGGCCGTG 
CTGGCCCCCC TGCAGGACGT GGACGTGGGG GCCGGGGAGA TGGCGCTGTT TGAGTGCCTG 
GTGGCAGGTC CAGCTGACGT CGAGGTGGAC TGGCTGTGCC GGGGCCGCCT GCTGCAGCCT 
GCGCTGCTCA AATGCAAGAT GCATTTTGAT GGCCGCAAGT GCAAGCTGCT GCTCACCTCT 
GTGCACGAGG ACGACAGTGG TGTCTACACC TGCAAGCTGA GCACAGCCAA AGATGAACTG 
ACCTGCAGTG CCCGGCTGAC GGTTCGGCCG TCGCTGGCGC CTCTCTTCAC CCGGTTGCTG 
GAAGATGTGG AGGTGTTGGA AGGCCGTGCT GCCCGCTTGG ATTGCAAGAT CAGCGGTACC 
CCGCCTCCCT CCGTTACCTG GACTCATTTT GGCCACCCCG TGAATGAGGG TGACAACTTG 
CGGCTTCAAC AGGATGGAGG TCTACACTCA CTGCACATTG CCCGGGTGGG CAGTGAGGAC 
GAAGGACTCT ATGAAGTCAG TGCCACCAAC ACTCATGGCC AGGCCCACTG CTCCGCCCAG 
CTGTATGTGG AGGAGCCTCG GACAGCTGCC TCTGGCCCCA GCTCGAAGCT GGAGAAGATG 
CCATCTATCC CTGAGGAGCC GGAGCACGGA GATCTAGAGA GGCTGTCTAT TCCTGACTTC 
CTACGGCCAC TGCAAGATCT GGAAGTGGGA CTAGCTAAGG AGGCCATGCT GGAGTGCCAA 
GTGACCGGCC TGCCCTATCC CACCATCAGC TGGTTCCACA ATGGCCACCG CATTCAAAGC 
AGTGACGACC GACGCATGAC ACAGTACAGG GACATACATC GCCTGGTGTT CCCAGCTGTG 
GGGCCTCAGC ATGCTGGTGT CTATAAGAGT GTAATCGCCA ACAAGCTGGG CAAAGCGGCC 
TGCTACGCCC ATCTCTATGT CACAGATGTG GTTCCAGGCC CTCCAGATGG TGCTCCGGAA 
GTAGTAGCTG TGACCGGAAG GATGGTCACA CTATCGTGGA ACCCACCCAG GAGTCTGGAC 
ATGGCTATTG ACCCAGATTC CCTAACATAC ACTGTGCAGC ACCAGGTGTT GGGCTCGGAC 
CAGTGGACGG CACTGGTCAC AGGCCTGCGG GAGCCTGCGT GGGCAGCCAC AGGGCTGAAG 
AAGGGGATCC AGCACATCTT CCGGGTCCTC AGCTCCAGTG GCAAGAGCAG CAGCAAGCCC 
TCAGCACCGT CGGAACCCGT GCAGCTGCTA GAGCATGGCC CACCCCTGGA AGAGGCGCCT 
GCTGTGCTGG ACAAGCAGGA CATTGTGTAT GTGGTAGAGG GCCAGCCTGC CTGTGTCACC 
GTCACTTTTA ACCATGTGGA GGCCCAGGTT GTCTGGAGGA GCTGCCGGGG AGCCCTCCTC 
GAGGCGCGCA CAGGTGTGTA TGAACTGAGT CAGCCAGATG ACGACCAGTA TTGTCTGCGG 
ATCTGCCGGG TGAGCCGACG AGACCTGGGA CCCCTTACTT GCAGTGCCCG AAACCGCCAC 
GGCACAAAGG CCTGCTCGGT CACCCTGGAG CTGGCAGAGG CACCTCGGTT TGAGTCTATC 
ATGGAAGATG TGGAGGTGGG GCCTGGAGAA ACCGCTCGCT TTGCTGTGGT AGTTGAGGGG 
AAACCACTGC CAGACATCAT GTGGTACAAG GATGAGGTGC TGCTGGCCGA GAGCAACCAT 
GTGAGCTTCG TGTATGAGGA GAACGAGTGT TCCCTAGTGC TGCTCAGCGC AGGAAGCCAG 
GATGGAGGCG TCTATACCTG TACCGCCCGG AACCTGGCAG GCGAAGTCTC CTGCAAGGCG 
GAGCTGTCTG TGCTTTCAGC TCAGACAGCC ATGGAGGTCG AGGGGGTCGG AGAGGATGAG 
GAACACCGAG GAAGAAGACT TAGCGACTAC TACGACATCC ACCAAGAGAT TGGCAGGGGT 
GCCTTCTCCT ACCTGCGGCG TGTAGTGGAG AGGAGCTCTG GCCTGGAGTT TGCAGCCAAG 
TTCATCCCTA GCCAGGCTAA GCCTAAAGCA TCAGCAAGAC GCGAGGCGCG CTTGCTGGCC 
CGGCTCCAGC ATGGCTGTGT GCTCTACTTC CACGAAGCCT TTGAGAGGCG CAGGGGACTT 
GTCATAGTCA CGGAGCTCTG CACAGAAGAG CTTCTAGAGC GCATGGCCAG GAAGCCCACC 
GTGTGTGAGT CTGAGACTCG GACCTATATG CGTCAAGTGC TAGAGGGGAT ATGCTATCTG 
CATCAGAGCC ATGTGCTGCA CCTGGATGTC AAGCCGGAGA ACCTGCTTGT GTGGGATGGT 
GCAGGGGGTG AAGAGCAGGT GCGCATCTGT GACTTCGGGA ACGCCCAGGA ACTCACTCCG 
GGAGAGCCCC AGTACTGTCA GTATGGCACG CCAGAGTTTG TAGCACCTGA GATTGTCAAC 
CAGAGCCCTG TGTCTGGAGT CACTGACATC TGGCCCGTGG GCGTCGTTGC CTTTCTCTGT 
TTGACGGGCA TCTCCCCATT TGTTGGGGAG AATGACCGGA CAACACTCAT GAATATCCGA 
AACTACAACG TGGCATTTGA GGAGACTACG TTCCTGAGTC TGAGCAGGGA GGCCCGGGGC 
TTCCTCATCA AAGTGCTGGT GCAGGATCGA CTGAGACCTA CTGCAGAAGA GACATTGGAA 
CATCCTTGGT TCAAAACAGA AGCAAAGGGT GCAGAGGTGA GCACAGATCA CCTGAAGCTC 
TTCCTGTCAC GGAGGAGGTG GCAGCGCTCT CAAATCAGCT ACAAGTGCCA CCTGGTGCTG 
CGCCCCATTC CGGAACTGCT ACGGGCTCCC CCGGAGCGGG TGTGGGTGGC CATGCCTAGA 
AGACAACCCC CGAGTGGGGG ACTCTCATCC TCTTCAGACT CAGAAGAGGA AGAGTTGGAG 
GAACTTCCCT CAGTACCCCG CCCCCTGCAG CCTGAGTTCT CAGGTTCTCG GGTATCCCTC 
ACCGACATCC CCACCGAAGA TGAGGCTCTG GGGACCCCGG AAGCTGGGGC TGCCACCCCC 
ATGGACTGGC AAGAGCAGGA AAGGACTCCC TCTAAGGACC AGGAGGCCCC CAGCCCTGAG 
GCACTCCCCT CTCCAGGCCA GGAGTCCCCT GATGGGCCTA GCCCCAGGCG GCCAGAGCTC 
CGCAGGGGTA GCTCTGCTGA GAGTGCCCTG CCCCGAGTCG GGTCGCGGGA ACCGGGCCGG 
AGCTTGCACA AGGCAGCATC CGTGGAGCTG CCGCAGCGCA GGAGCCCCAG CCCGGGTGCC 
ACCCGCCTGA CTCGGGGAGG CCTGGGTGAG GGCGAGTATG CCCAGAGGCT GCAGGCCCTG 
CGTCAGCGGC TGCTGAGGGG AGGCCCCGAG GATGGCAAGG TCAGCGGCCT CAGGGGACCC 
CTGCTGGAGA GCCTGGGGGG CCGTGCACGG GATCCCAGGA TGGCAAGGGC TGCCTCCAGC 
GAGGCTGCCC CCCACCACCA GCCCCCTCCG GAGTCCCGGG GTCTGCAAAA GAGCAGCAGC 
TTCTCTCAGG GCGAGGCAGA GCCCCGGGGT CGGCACCGCC GCGCGGGGGC ACCCCTCGAA 
ATCCCCGTGG CCAGACTTGG GGCTCGCAGG CTGCAGGAGT CTCCCTCCTT ATCTGCCCTT 
AGCGAGACCC AGCCACCGAG CCCTGCGAGG CCCAGCGTCC CCAAACTCAG TATCACCAAG 
TCCCCGGAAC CTTCCGCTGT CACATCCAGA GACTCTCCCC AGCCCCCGGA ACCTCAGCCT 
GTCCCAGAAA AGGTCCCTGA GCCCAAACCA GAACCAGTCC GAGCTGCAAA ACCAGCACAA 
CCCCCTTTGG CTCTGCAAAT GCCAACGCAG CCTCTCACCC CCTATGCCCA GATCATGCAG 
TCGCTCCAGC TGTCCAGTCC CACCCTGTCC CCACAGGACC CCGCTGTGCC TCCATCAGAG 
CCCAAGCCCC ACGCGGCTGT GTTTGCCAGG GTGGCTTCCC CACCACCCGG AGTCTCTGAG 
AAGCGCGTGC CTTCGGCCAG AACTCCCCCA GTGCTAGCCG AGAAAGCCCG AGTCCCCACG 
GTGCCCCCCA GGCCAGGCAG CAGCCTCAGC GGCAGCATCG AAAACCTTGA ATCAGAGGCT 
GTGTTCGAGG CTAAGTTCAA ACGCAGCCGC GAATCGCCAC TGTCGCGGGG CCTGAGGCTA 
CTGAGCCGCT CGCGCTCGGA GGAGCGCGGT CCCTTCCGTG GGGCGGAGGA CGACGGCATA 
TACCGGCCCA GTCCGGCAGG AACGCCGCTA GAGCTGGTGC GACGGCCCGA GCGCTCGCGC 
TCCGTGCAGG ACCTCAGGGT AGCCGGAGAG CCGGGCCTTG TGCGTCGCCT TTCGCTGTCC 
CTGTCGCAGA AGCTGCGGCG GACACCGCCG GGACAGCGAC ACCCTGCCTG GGAGTCCCGC 
AGCGGAGACG GGGAGAGCTC CGAGGGCGGC AGCTCAGCTC GGGCATCCCC AGTGCTGGCG 
GTACGCAGGA GACTCAGCTC CACGCTGGAG CGTCTGTCCA GCCGTTTGCA GCGCAGCGGC 
AGCAGCGAGG ACTCCGGGGG CGCCTCGGGT CGCAGCACGC CGCTGTTTGG ACGGCTGCGC 
AGGGCTACTT CGGAAGGGGA GAGCCTGCGA CGCCTCGGTG TTCCGCACAA TCAGCTGGGC 
TCTCAGACTG GTGCCACTAC ACCTTCCGCT GAGTCTCTGG GCTCAGAGGC CAGTGGCACA 
TCGGGTTCTT CAGCTCCTGG AGAAAGCCGA AGCCGGCACC GGTGGGGCCT TTCCAGGCTG 
CGCAAGGACA AGGGATTATC TCAGCCAAAC CTCTCCTCCA GTGTCCAAGA GGACTTGGGT 
CACCAGTATG TGCCCAGTGA GTCAGATTTC CCCCCAGTCT TCCACATCAA ACTCAAGGAC 
CAGGTGCTGT TGGAGGGGGA AGCAGCCACC CTGCTCTGCC TACCCGCAGC CTGCCCAGCA 
CCCCGCATCT CCTGGATGAA AGATAAGCAG TCCCTGCGTT CAGAGCCCTC AGTGGTCATT 
GTGTCCTGCA AAGATGGACG GCAGCTGCTG AGCATACCCC GGGCCGGCAA GCGGCATGCT 
GGGCTCTATG AGTGCTCAGC CACCAATGTC CTGGGCAGCA TTACCAGCTC CTGTACTGTG 
GCCGTGGCCC GCATTCCAGG GAAGCTAGCT CCTCCAGAGG TGCCCCAGAC CTACCATGAC 
ACAGCACTAG TAGTGTGGAA GCCAGGAGAC GGTCGGGCAC CTTGCACATA CACTCTGGAG 
CGGCGGGTGG ATGGGGAATC TGTCTGGCAC CCTGTGAGCT CAGGCATCCC CGACTGTTAT 
TACAATGTGA CTCAGCTGCC TGTCGGCGTG ACCGTGCGGT TCCGTGTGGC CTGTTCCAAT 
CGTGCTGGGC AGGGGCCCTT CAGCAACCCT TCTGAGAAGG TCTTCATCCG AGGCACTCCA 
GATTCTCCTG CTCAGCCAGC TGCTGCTCCC CGAGATGCCC CTGTTACCTC AGGGCCAACC 
AGGGCCCCAC CGCCTGACTC TCCTACCTCA CTGGCCCCCA CCCCCGCTCT TGCGCCCCCA 
GCCTCCCAGG CATCCACTCT CAGCCCCTCA ACTTCCTCCA TGTCTGCCAA CCAGGCCTTG 
TCCTCACTCA AGGCTGTGGG TCCACCACCC GCAACCCCTC CACGAAAGCA CAGGGGCCTT 
CTGGCCACCC AGCAAGCAGA GCCATCCCCA CCCAGTATCG TGGTCACCCC AAGTGAACCC 
AGGTCTTTTG TCCCTGACAC TGGAACCCTG ACCCCAACCT CTAGTCCTCA AGGGGTTAAA 
CCAGCACCTT CCTCAACTTC CTTGTATATG GTGACTTCCT TCGTGTCTGC ACCTCCAGCC 
CCCCAGGCCC CAGCCCCTGA GCCCCCTCCT GAGCCCACCA AGGTGACTGT GCGGAGCCTC 
AGCCCAGCCA AGGAGGTGGT CAGCTCCCCA ACTCCTGAGA GCACCACTCT TCGGCAGGGT 
CCCCCTCAGA AACCCTACAC CTTCCTGGAG GAGAAGGCCA GGGGCCGCTT TGGCGTTGTG 
CGGTCATGCC GGGAGAATGC TACGGGCCGA ACGTTTGTCG CCAAGATCGT GCCGTACGCC 
GCGGAGGGCA AGCGACGCGT GCTGCAGGAA TACGAGGTGC TTCGGACGCT GCACCACGAG 
CGGCTCATGT CCCTGCACGA GGCCTACATC ACCCCTCGGT ACCTCGTGCT TATCGCTGAG 
AGCTGTGGCA ACCGGGAACT CCTCTGTGGG CTCAGCGACA GATTCCGGTA TTCAGAAGAT 
GACGTGGCCA CCTATGTGGT GCAGCTGCTA CAAGGCCTGG ACTACCTCCA CGGCCACCAC 
GTGCTACACC TGGACATCAA GCCGGACAAT CTACTGTTGG CCGCTGACAA TGCCCTTAAA 
ATTGTGGACT TCGGCAGTGC TCAGCCCTAT AACCCTCAGG CCCTGAAGCC ACTTGGCCAC 
CGCACAGGCA CTCTGGAGTT CATGGCTCCT GAGATGGTGA AGGGAGACCC CATTGGCTCT 
GCCACGGACA TCTGGGGAGC GGGTGTGCTC ACCTACATCA TGCTTAGTGG GTACTCCCCA 
TTCTATGAGC CAGACCCCCA GGAAACAGAG GCTCGGATTG TTGGGGGTCG CTTTGATGCC 
TTCCAGTTGT ATCCTAACAC ATCCCAGAGT GCCACCCTCT TCTTGAGAAA GGTCCTCTCA 
GTACATCCCT GGAGCCGGCC CTCTCTGCAG GACTGCTTGG CCCACCCATG GCTGCAAGAT 
GCCTACCTGA TGAAGCTGCG CCGCCAGACA CTCACCTTCA CCACCAACCG GCTCAAGGAA 
TTCCTGGGCG AGCAGCGGCG ACGTCGGGCT GAGGCTGCTA CCCGTCACAA GGTGCTGCTC 
CGCTCCTACC CTGGCAGCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Speg ( XM_030243202.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099062.1 Mus musculus SPEG complex locus (Speg), transcript variant X2, mRNA.

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CloneID	OMu84984
Clone ID Related Accession (Same CDS sequence)	XM_030243202.1
Accession Version	XM_030243202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	striated muscle-specific serine/threonine-protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
TACGACTCGG AGACTGCCGA GGATGACATC AGCGATGTGC CGGGGACCCA GCGCCTGGAG 
CTTCGGGATG ACAGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGTACC 
TCCCTGGACA CACCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG 
GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC 
CCAGGCAGCC CAAGGCAAGC ACAGACAACC GGGGCCGGGC CACGGCACCT GGGGGTGGAG 
CCGCTGGTGC GGGCATCGCG AGCTAATCTG GTGGGCGCAA GCTGGGGGTC AGAGGATAGC 
CTTTCGGTGG CCAGTGACCT CTATGGCAGC GCGTTCAGTC TGTACAGAGG ACGGGCGCTC 
TCGATCCACG TCAGCATTCC TCCGAGTGGA TTGCACAGAG AGGAGCCTGA CCTTCAGCCT 
CAGCCAGCCA GTGATGCCCT TCGTCCACGC CCTGCCCTTC CACCTCCCTC CAAATCCGCT 
TTGCTTCCCC CACCATCTCC TCGGGTGGGA AAGAGGGCTC TGCCAGGACC TAGTACCCAG 
CCCCCAGCTA CTCCCACTTC GCCCCATCGG CGTGCTCAGG AGCCCTCGCT GCCTGAGGAC 
ATCACCACCA CGGAAGAGAA GCGAGGGAAA AAGCCCAAGT CATCCGGGCC GTCGCTGGCG 
GGCACAGTGG AGTCCCGGCC CCAGACGCCA CTGAGCGAGG CTTCGGGTCG CCTGTCAGCA 
TTGGGCCGCT CGCCCCGGCT GGTGCGCGCG GGGTCCCGCA TCCTGGACAA GCTACAGTTC 
TTCGAAGAGC GGCGACGCAG CCTGGAGCGC AGCGACTCGC CGCCAGCGCC CCTGCGGCCC 
TGGGTGCCCC TGCGCAAGGC TCGCTCGCTG GAGCAGCCGA AGTCCGAGGG CGGTGCGGCG 
TGGGGCACAC CCGAGGCCTC GCAGGAGGAG CTGCGGTCAC CTCGGGGCAG TGTGGCAGAG 
CGGCGTCGCC TGTTCCAGCA AAAGGCGGCC TCGTTGGATG AACGCACGCG ACAACGCAGT 
GCAACCTCGG ACCTCGAACT CCGCTTCGCC CAGGAGCTGG GTCGCATCCG CCGATCTACG 
TCGCGGGAGG AGCTGGTGCG TTCGCACGAG TCCCTGCGTG CCACGCTGCA GCGCGCCCCA 
TCCCCTCGGG AGCCCGGCGA GCCCCCACTC TTCTCCCGGC CTTCCACACC CAAGACCTCA 
CGGGCTGTGA GCCCGGCTGC CACCCAGCCG CCGCCTCCTA GTGGTGCGGG CAAATCTGGG 
GACGAGCCTG GGAGGCCCCG AAGCAGAGGG CCGGTGGGCA GGACTGAACC GGGGGAAGGC 
CCGCAGCAGG AGATCAAGCG TCGGGACCAA TTCCCGCTAA CCAGGAGCAG AGCCATCCAG 
GAGTGCAGGA GCCCTGTGCC GCCCTACACC GCGGATCCCC CGGAGAGCAG GACAAAAGCC 
CCCTCCGGTC GCAAGCGGGA ACCCCCTGCT CAAGCGGTGC GCTTTCTGCC CTGGGCCACT 
CCGGGAGTGG AGGACTCTGT TCTGCCCCAA ACCTTGGAGA AGAATAGAGC GGGACCCGAG 
GCTGAGAAGA GGCTTCGCAG AGGACCTGAG GAGGATGGCC CCTGGGGGCC CTGGGACCGC 
AGAGGGACCC GCAGCCAAGG CAAAGGTCGC CGTGCTCGGC CTACTTCCCC CGAGCTCGAG 
TCCTCAGACG ACTCCTATGT GTCCGCTGGG GAAGAGCCCC TGGAGGCACC CGTGTTTGAG 
ATCCCTCTGC AGAATATGGT GGTGGCGCCA GGAGCTGACG TGCTACTTAA GTGTATCATC 
ACCGCCAACC CCCCACCCCA AGTGTCCTGG AAAAAGGATG GGTCCATGTT GCACAGCGAG 
GGTCGTCTTC TCATCCGGGC TGAAGGTGAA CGGCACACAC TGCTGCTCAG AGAGGCCCAG 
GCTGCCGATG CTGGGAGCTA CACAGCCACT GCCACCAACG AACTGGGCCA AGCTACCTGT 
GCTTCTTCAC TGGCTGTGAG ACCTGGCGGC TCCACATCCC CTTTCAGCAG CCCCATCACC 
TCTGATGAGG AGTACCTGAG CCCCCCAGAG GAGTTCCCAG AGCCTGGGGA GACCTGGCCC 
CGAACCCCTA CCATGAAGCT CAGTCCCAGC CAGGATCATG ATTCCTCCGA CTCTTCTTCC 
AAGGCACCCC CAACGTTCAA GGTCTCACTC ATGGACCAAT CGGTGAGAGA AGGTCAAGAT 
GTCATTATGA GCATCCGTGT GCAGGGAGAG CCCAAGCCTG TGGTTTCCTG GCTGAGGAAT 
CGACAGCCCG TGCGCCCAGA CCAGCGGCGC TTTGCAGAGG AGGCCGAGGG TGGGCTCTGC 
CGCTTGAGGA TCCTGGCTGC TGAACGGGGC GATGCTGGTT TCTACACATG CAAGGCGGTC 
AACGAATATG GCGCTCGGCA GTGCGAGGCC CGCCTGGAGG TCCGAGCACA CCCTGAAAGC 
CGGTCCCTGG CCGTGCTGGC CCCCCTGCAG GACGTGGACG TGGGGGCCGG GGAGATGGCG 
CTGTTTGAGT GCCTGGTGGC AGGTCCAGCT GACGTCGAGG TGGACTGGCT GTGCCGGGGC 
CGCCTGCTGC AGCCTGCGCT GCTCAAATGC AAGATGCATT TTGATGGCCG CAAGTGCAAG 
CTGCTGCTCA CCTCTGTGCA CGAGGACGAC AGTGGTGTCT ACACCTGCAA GCTGAGCACA 
GCCAAAGATG AACTGACCTG CAGTGCCCGG CTGACGGTTC GGCCGTCGCT GGCGCCTCTC 
TTCACCCGGT TGCTGGAAGA TGTGGAGGTG TTGGAAGGCC GTGCTGCCCG CTTGGATTGC 
AAGATCAGCG GTACCCCGCC TCCCTCCGTT ACCTGGACTC ATTTTGGCCA CCCCGTGAAT 
GAGGGTGACA ACTTGCGGCT TCAACAGGAT GGAGGTCTAC ACTCACTGCA CATTGCCCGG 
GTGGGCAGTG AGGACGAAGG ACTCTATGAA GTCAGTGCCA CCAACACTCA TGGCCAGGCC 
CACTGCTCCG CCCAGCTGTA TGTGGAGGAG CCTCGGACAG CTGCCTCTGG CCCCAGCTCG 
AAGCTGGAGA AGATGCCATC TATCCCTGAG GAGCCGGAGC ACGGAGATCT AGAGAGGCTG 
TCTATTCCTG ACTTCCTACG GCCACTGCAA GATCTGGAAG TGGGACTAGC TAAGGAGGCC 
ATGCTGGAGT GCCAAGTGAC CGGCCTGCCC TATCCCACCA TCAGCTGGTT CCACAATGGC 
CACCGCATTC AAAGCAGTGA CGACCGACGC ATGACACAGT ACAGGGACAT ACATCGCCTG 
GTGTTCCCAG CTGTGGGGCC TCAGCATGCT GGTGTCTATA AGAGTGTAAT CGCCAACAAG 
CTGGGCAAAG CGGCCTGCTA CGCCCATCTC TATGTCACAG ATGTGGTTCC AGGCCCTCCA 
GATGGTGCTC CGGAAGTAGT AGCTGTGACC GGAAGGATGG TCACACTATC GTGGAACCCA 
CCCAGGAGTC TGGACATGGC TATTGACCCA GATTCCCTAA CATACACTGT GCAGCACCAG 
GTGTTGGGCT CGGACCAGTG GACGGCACTG GTCACAGGCC TGCGGGAGCC TGCGTGGGCA 
GCCACAGGGC TGAAGAAGGG GATCCAGCAC ATCTTCCGGG TCCTCAGCTC CAGTGGCAAG 
AGCAGCAGCA AGCCCTCAGC ACCGTCGGAA CCCGTGCAGC TGCTAGAGCA TGGCCCACCC 
CTGGAAGAGG CGCCTGCTGT GCTGGACAAG CAGGACATTG TGTATGTGGT AGAGGGCCAG 
CCTGCCTGTG TCACCGTCAC TTTTAACCAT GTGGAGGCCC AGGTTGTCTG GAGGAGCTGC 
CGGGGAGCCC TCCTCGAGGC GCGCACAGGT GTGTATGAAC TGAGTCAGCC AGATGACGAC 
CAGTATTGTC TGCGGATCTG CCGGGTGAGC CGACGAGACC TGGGACCCCT TACTTGCAGT 
GCCCGAAACC GCCACGGCAC AAAGGCCTGC TCGGTCACCC TGGAGCTGGC AGAGGCACCT 
CGGTTTGAGT CTATCATGGA AGATGTGGAG GTGGGGCCTG GAGAAACCGC TCGCTTTGCT 
GTGGTAGTTG AGGGGAAACC ACTGCCAGAC ATCATGTGGT ACAAGGATGA GGTGCTGCTG 
GCCGAGAGCA ACCATGTGAG CTTCGTGTAT GAGGAGAACG AGTGTTCCCT AGTGCTGCTC 
AGCGCAGGAA GCCAGGATGG AGGCGTCTAT ACCTGTACCG CCCGGAACCT GGCAGGCGAA 
GTCTCCTGCA AGGCGGAGCT GTCTGTGCTT TCAGCTCAGA CAGCCATGGA GGTCGAGGGG 
GTCGGAGAGG ATGAGGAACA CCGAGGAAGA AGACTTAGCG ACTACTACGA CATCCACCAA 
GAGATTGGCA GGGGTGCCTT CTCCTACCTG CGGCGTGTAG TGGAGAGGAG CTCTGGCCTG 
GAGTTTGCAG CCAAGTTCAT CCCTAGCCAG GCTAAGCCTA AAGCATCAGC AAGACGCGAG 
GCGCGCTTGC TGGCCCGGCT CCAGCATGGC TGTGTGCTCT ACTTCCACGA AGCCTTTGAG 
AGGCGCAGGG GACTTGTCAT AGTCACGGAG CTCTGCACAG AAGAGCTTCT AGAGCGCATG 
GCCAGGAAGC CCACCGTGTG TGAGTCTGAG ACTCGGACCT ATATGCGTCA AGTGCTAGAG 
GGGATATGCT ATCTGCATCA GAGCCATGTG CTGCACCTGG ATGTCAAGCC GGAGAACCTG 
CTTGTGTGGG ATGGTGCAGG GGGTGAAGAG CAGGTGCGCA TCTGTGACTT CGGGAACGCC 
CAGGAACTCA CTCCGGGAGA GCCCCAGTAC TGTCAGTATG GCACGCCAGA GTTTGTAGCA 
CCTGAGATTG TCAACCAGAG CCCTGTGTCT GGAGTCACTG ACATCTGGCC CGTGGGCGTC 
GTTGCCTTTC TCTGTTTGAC GGGCATCTCC CCATTTGTTG GGGAGAATGA CCGGACAACA 
CTCATGAATA TCCGAAACTA CAACGTGGCA TTTGAGGAGA CTACGTTCCT GAGTCTGAGC 
AGGGAGGCCC GGGGCTTCCT CATCAAAGTG CTGGTGCAGG ATCGACTGAG ACCTACTGCA 
GAAGAGACAT TGGAACATCC TTGGTTCAAA ACAGAAGCAA AGGGTGCAGA GGTGAGCACA 
GATCACCTGA AGCTCTTCCT GTCACGGAGG AGGTGGCAGC GCTCTCAAAT CAGCTACAAG 
TGCCACCTGG TGCTGCGCCC CATTCCGGAA CTGCTACGGG CTCCCCCGGA GCGGGTGTGG 
GTGGCCATGC CTAGAAGACA ACCCCCGAGT GGGGGACTCT CATCCTCTTC AGACTCAGAA 
GAGGAAGAGT TGGAGGAACT TCCCTCAGTA CCCCGCCCCC TGCAGCCTGA GTTCTCAGGT 
TCTCGGGTAT CCCTCACCGA CATCCCCACC GAAGATGAGG CTCTGGGGAC CCCGGAAGCT 
GGGGCTGCCA CCCCCATGGA CTGGCAAGAG CAGGAAAGGA CTCCCTCTAA GGACCAGGAG 
GCCCCCAGCC CTGAGGCACT CCCCTCTCCA GGCCAGGAGT CCCCTGATGG GCCTAGCCCC 
AGGCGGCCAG AGCTCCGCAG GGGTAGCTCT GCTGAGAGTG CCCTGCCCCG AGTCGGGTCG 
CGGGAACCGG GCCGGAGCTT GCACAAGGCA GCATCCGTGG AGCTGCCGCA GCGCAGGAGC 
CCCAGCCCGG GTGCCACCCG CCTGACTCGG GGAGGCCTGG GTGAGGGCGA GTATGCCCAG 
AGGCTGCAGG CCCTGCGTCA GCGGCTGCTG AGGGGAGGCC CCGAGGATGG CAAGGTCAGC 
GGCCTCAGGG GACCCCTGCT GGAGAGCCTG GGGGGCCGTG CACGGGATCC CAGGATGGCA 
AGGGCTGCCT CCAGCGAGGC TGCCCCCCAC CACCAGCCCC CTCCGGAGTC CCGGGGTCTG 
CAAAAGAGCA GCAGCTTCTC TCAGGGCGAG GCAGAGCCCC GGGGTCGGCA CCGCCGCGCG 
GGGGCACCCC TCGAAATCCC CGTGGCCAGA CTTGGGGCTC GCAGGCTGCA GGAGTCTCCC 
TCCTTATCTG CCCTTAGCGA GACCCAGCCA CCGAGCCCTG CGAGGCCCAG CGTCCCCAAA 
CTCAGTATCA CCAAGTCCCC GGAACCTTCC GCTGTCACAT CCAGAGACTC TCCCCAGCCC 
CCGGAACCTC AGCCTGTCCC AGAAAAGGTC CCTGAGCCCA AACCAGAACC AGTCCGAGCT 
GCAAAACCAG CACAACCCCC TTTGGCTCTG CAAATGCCAA CGCAGCCTCT CACCCCCTAT 
GCCCAGATCA TGCAGTCGCT CCAGCTGTCC AGTCCCACCC TGTCCCCACA GGACCCCGCT 
GTGCCTCCAT CAGAGCCCAA GCCCCACGCG GCTGTGTTTG CCAGGGTGGC TTCCCCACCA 
CCCGGAGTCT CTGAGAAGCG CGTGCCTTCG GCCAGAACTC CCCCAGTGCT AGCCGAGAAA 
GCCCGAGTCC CCACGGTGCC CCCCAGGCCA GGCAGCAGCC TCAGCGGCAG CATCGAAAAC 
CTTGAATCAG AGGCTGTGTT CGAGGCTAAG TTCAAACGCA GCCGCGAATC GCCACTGTCG 
CGGGGCCTGA GGCTACTGAG CCGCTCGCGC TCGGAGGAGC GCGGTCCCTT CCGTGGGGCG 
GAGGACGACG GCATATACCG GCCCAGTCCG GCAGGAACGC CGCTAGAGCT GGTGCGACGG 
CCCGAGCGCT CGCGCTCCGT GCAGGACCTC AGGGTAGCCG GAGAGCCGGG CCTTGTGCGT 
CGCCTTTCGC TGTCCCTGTC GCAGAAGCTG CGGCGGACAC CGCCGGGACA GCGACACCCT 
GCCTGGGAGT CCCGCAGCGG AGACGGGGAG AGCTCCGAGG GCGGCAGCTC AGCTCGGGCA 
TCCCCAGTGC TGGCGGTACG CAGGAGACTC AGCTCCACGC TGGAGCGTCT GTCCAGCCGT 
TTGCAGCGCA GCGGCAGCAG CGAGGACTCC GGGGGCGCCT CGGGTCGCAG CACGCCGCTG 
TTTGGACGGC TGCGCAGGGC TACTTCGGAA GGGGAGAGCC TGCGACGCCT CGGTGTTCCG 
CACAATCAGC TGGGCTCTCA GACTGGTGCC ACTACACCTT CCGCTGAGTC TCTGGGCTCA 
GAGGCCAGTG GCACATCGGG TTCTTCAGCT CCTGGAGAAA GCCGAAGCCG GCACCGGTGG 
GGCCTTTCCA GGCTGCGCAA GGACAAGGGA TTATCTCAGC CAAACCTCTC CTCCAGTGTC 
CAAGAGGACT TGGGTCACCA GTATGTGCCC AGTGAGTCAG ATTTCCCCCC AGTCTTCCAC 
ATCAAACTCA AGGACCAGGT GCTGTTGGAG GGGGAAGCAG CCACCCTGCT CTGCCTACCC 
GCAGCCTGCC CAGCACCCCG CATCTCCTGG ATGAAAGATA AGCAGTCCCT GCGTTCAGAG 
CCCTCAGTGG TCATTGTGTC CTGCAAAGAT GGACGGCAGC TGCTGAGCAT ACCCCGGGCC 
GGCAAGCGGC ATGCTGGGCT CTATGAGTGC TCAGCCACCA ATGTCCTGGG CAGCATTACC 
AGCTCCTGTA CTGTGGCCGT GGCCCGCATT CCAGGGAAGC TAGCTCCTCC AGAGGTGCCC 
CAGACCTACC ATGACACAGC ACTAGTAGTG TGGAAGCCAG GAGACGGTCG GGCACCTTGC 
ACATACACTC TGGAGCGGCG GGTGGATGGG GAATCTGTCT GGCACCCTGT GAGCTCAGGC 
ATCCCCGACT GTTATTACAA TGTGACTCAG CTGCCTGTCG GCGTGACCGT GCGGTTCCGT 
GTGGCCTGTT CCAATCGTGC TGGGCAGGGG CCCTTCAGCA ACCCTTCTGA GAAGGTCTTC 
ATCCGAGGCA CTCCAGATTC TCCTGCTCAG CCAGCTGCTG CTCCCCGAGA TGCCCCTGTT 
ACCTCAGGGC CAACCAGGGC CCCACCGCCT GACTCTCCTA CCTCACTGGC CCCCACCCCC 
GCTCTTGCGC CCCCAGCCTC CCAGGCATCC ACTCTCAGCC CCTCAACTTC CTCCATGTCT 
GCCAACCAGG CCTTGTCCTC ACTCAAGGCT GTGGGTCCAC CACCCGCAAC CCCTCCACGA 
AAGCACAGGG GCCTTCTGGC CACCCAGCAA GCAGAGCCAT CCCCACCCAG TATCGTGGTC 
ACCCCAAGTG AACCCAGGTC TTTTGTCCCT GACACTGGAA CCCTGACCCC AACCTCTAGT 
CCTCAAGGGG TTAAACCAGC ACCTTCCTCA ACTTCCTTGT ATATGGTGAC TTCCTTCGTG 
TCTGCACCTC CAGCCCCCCA GGCCCCAGCC CCTGAGCCCC CTCCTGAGCC CACCAAGGTG 
ACTGTGCGGA GCCTCAGCCC AGCCAAGGAG GTGGTCAGCT CCCCAACTCC TGAGAGCACC 
ACTCTTCGGC AGGGTCCCCC TCAGAAACCC TACACCTTCC TGGAGGAGAA GGCCAGGGGC 
CGCTTTGGCG TTGTGCGGTC ATGCCGGGAG AATGCTACGG GCCGAACGTT TGTCGCCAAG 
ATCGTGCCGT ACGCCGCGGA GGGCAAGCGA CGCGTGCTGC AGGAATACGA GGTGCTTCGG 
ACGCTGCACC ACGAGCGGCT CATGTCCCTG CACGAGGCCT ACATCACCCC TCGGTACCTC 
GTGCTTATCG CTGAGAGCTG TGGCAACCGG GAACTCCTCT GTGGGCTCAG CGACAGATTC 
CGGTATTCAG AAGATGACGT GGCCACCTAT GTGGTGCAGC TGCTACAAGG CCTGGACTAC 
CTCCACGGCC ACCACGTGCT ACACCTGGAC ATCAAGCCGG ACAATCTACT GTTGGCCGCT 
GACAATGCCC TTAAAATTGT GGACTTCGGC AGTGCTCAGC CCTATAACCC TCAGGCCCTG 
AAGCCACTTG GCCACCGCAC AGGCACTCTG GAGTTCATGG CTCCTGAGAT GGTGAAGGGA 
GACCCCATTG GCTCTGCCAC GGACATCTGG GGAGCGGGTG TGCTCACCTA CATCATGCAC 
GCAACCAAAA GCAAAGTGGC ATTATCCCTG AATACTGTGC CCCTTCCAGA TGTCCTACAT 
GAATGTCTCT TAGACCTTTC AACTGCACAT CACTCTAAGG TAGAGGCAAT GAGAGAGGCA 
TCCGAGATGG CAATTCACCT TTCTTCCACA ACAGGCTTAG TGGGTACTCC CCATTCTATG 
AGCCAGACCC CCAGGAAACA GAGGCTCGGA TTGTTGGGGG TCGCTTTGAT GCCTTCCAGT 
TGTATCCTAA CACATCCCAG AGTGCCACCC TCTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099062.1
CDS	105..9863
Translation	MQKARGTRGEDAGTRAPPSPGVPPKRAKVGAGRGVLVTGDGAGAPVFLRPLKNAAVCAGSDVRLRVVVSGTPQPSLSWFRDGQLLPPPAPEPSCLWLRSCGAQDAGVYSCSAQNERGQASCEAVLTVLEVRVFRSCRKQSYDSETAEDDISDVPGTQRLELRDDRAFSTPTGGSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPGSPRQAQTTGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAFSLYRGRALSIHVSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALLPPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKPKSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFEERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELRSPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSREELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPPPSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQECRSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTLEKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESSDDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKKDGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCASSLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQDHDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQPVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARLEVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRLLQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLTVRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEGDNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPRTAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAMLECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGVYKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPRSLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIFRVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPACVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRRDLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVVVEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTCTARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEIGRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTEAKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVAMPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTEDEALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRRPELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGGLGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARAASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLGARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPEPQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSPTLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKARVPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSEERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRLSLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSSTLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHNQLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLSQPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAACPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSATNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTYTLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPFSNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPALAPPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAEPSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSAPPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYTFLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTLHHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVVQLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKPLGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMHATKSKVALSLNTVPLPDVLHECLLDLSTAHHSKVEAMREASEMAIHLSSTTGLVGTPHSMSQTPRKQRLGLLGVALMPSSCILTHPRVPPSS

Target ORF information:

RefSeq Version	XM_030243202.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPEG complex locus (Speg), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243202.1

ORF Insert Sequence:

ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCCGGCA CGAGGGCACC TCCTAGCCCC 
GGGGTGCCCC CGAAGAGAGC CAAGGTGGGG GCCGGCAGAG GGGTCCTCGT CACCGGGGAT 
GGGGCCGGGG CGCCGGTCTT CCTGCGGCCC CTGAAGAACG CGGCGGTGTG TGCGGGCAGT 
GATGTAAGGC TGCGGGTAGT AGTGAGCGGG ACACCCCAGC CTAGCCTCAG CTGGTTCCGG 
GATGGGCAGC TCCTGCCTCC ACCGGCCCCA GAGCCCAGCT GCCTGTGGCT GCGGAGCTGC 
GGGGCTCAGG ACGCCGGCGT GTACAGCTGC AGCGCCCAGA ATGAGCGGGG CCAGGCCTCC 
TGTGAGGCAG TGCTCACAGT GCTGGAGGTC AGAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
TACGACTCGG AGACTGCCGA GGATGACATC AGCGATGTGC CGGGGACCCA GCGCCTGGAG 
CTTCGGGATG ACAGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGTACC 
TCCCTGGACA CACCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG 
GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCG GGGGTGGCAC CCGCCCCCTC 
CCAGGCAGCC CAAGGCAAGC ACAGACAACC GGGGCCGGGC CACGGCACCT GGGGGTGGAG 
CCGCTGGTGC GGGCATCGCG AGCTAATCTG GTGGGCGCAA GCTGGGGGTC AGAGGATAGC 
CTTTCGGTGG CCAGTGACCT CTATGGCAGC GCGTTCAGTC TGTACAGAGG ACGGGCGCTC 
TCGATCCACG TCAGCATTCC TCCGAGTGGA TTGCACAGAG AGGAGCCTGA CCTTCAGCCT 
CAGCCAGCCA GTGATGCCCT TCGTCCACGC CCTGCCCTTC CACCTCCCTC CAAATCCGCT 
TTGCTTCCCC CACCATCTCC TCGGGTGGGA AAGAGGGCTC TGCCAGGACC TAGTACCCAG 
CCCCCAGCTA CTCCCACTTC GCCCCATCGG CGTGCTCAGG AGCCCTCGCT GCCTGAGGAC 
ATCACCACCA CGGAAGAGAA GCGAGGGAAA AAGCCCAAGT CATCCGGGCC GTCGCTGGCG 
GGCACAGTGG AGTCCCGGCC CCAGACGCCA CTGAGCGAGG CTTCGGGTCG CCTGTCAGCA 
TTGGGCCGCT CGCCCCGGCT GGTGCGCGCG GGGTCCCGCA TCCTGGACAA GCTACAGTTC 
TTCGAAGAGC GGCGACGCAG CCTGGAGCGC AGCGACTCGC CGCCAGCGCC CCTGCGGCCC 
TGGGTGCCCC TGCGCAAGGC TCGCTCGCTG GAGCAGCCGA AGTCCGAGGG CGGTGCGGCG 
TGGGGCACAC CCGAGGCCTC GCAGGAGGAG CTGCGGTCAC CTCGGGGCAG TGTGGCAGAG 
CGGCGTCGCC TGTTCCAGCA AAAGGCGGCC TCGTTGGATG AACGCACGCG ACAACGCAGT 
GCAACCTCGG ACCTCGAACT CCGCTTCGCC CAGGAGCTGG GTCGCATCCG CCGATCTACG 
TCGCGGGAGG AGCTGGTGCG TTCGCACGAG TCCCTGCGTG CCACGCTGCA GCGCGCCCCA 
TCCCCTCGGG AGCCCGGCGA GCCCCCACTC TTCTCCCGGC CTTCCACACC CAAGACCTCA 
CGGGCTGTGA GCCCGGCTGC CACCCAGCCG CCGCCTCCTA GTGGTGCGGG CAAATCTGGG 
GACGAGCCTG GGAGGCCCCG AAGCAGAGGG CCGGTGGGCA GGACTGAACC GGGGGAAGGC 
CCGCAGCAGG AGATCAAGCG TCGGGACCAA TTCCCGCTAA CCAGGAGCAG AGCCATCCAG 
GAGTGCAGGA GCCCTGTGCC GCCCTACACC GCGGATCCCC CGGAGAGCAG GACAAAAGCC 
CCCTCCGGTC GCAAGCGGGA ACCCCCTGCT CAAGCGGTGC GCTTTCTGCC CTGGGCCACT 
CCGGGAGTGG AGGACTCTGT TCTGCCCCAA ACCTTGGAGA AGAATAGAGC GGGACCCGAG 
GCTGAGAAGA GGCTTCGCAG AGGACCTGAG GAGGATGGCC CCTGGGGGCC CTGGGACCGC 
AGAGGGACCC GCAGCCAAGG CAAAGGTCGC CGTGCTCGGC CTACTTCCCC CGAGCTCGAG 
TCCTCAGACG ACTCCTATGT GTCCGCTGGG GAAGAGCCCC TGGAGGCACC CGTGTTTGAG 
ATCCCTCTGC AGAATATGGT GGTGGCGCCA GGAGCTGACG TGCTACTTAA GTGTATCATC 
ACCGCCAACC CCCCACCCCA AGTGTCCTGG AAAAAGGATG GGTCCATGTT GCACAGCGAG 
GGTCGTCTTC TCATCCGGGC TGAAGGTGAA CGGCACACAC TGCTGCTCAG AGAGGCCCAG 
GCTGCCGATG CTGGGAGCTA CACAGCCACT GCCACCAACG AACTGGGCCA AGCTACCTGT 
GCTTCTTCAC TGGCTGTGAG ACCTGGCGGC TCCACATCCC CTTTCAGCAG CCCCATCACC 
TCTGATGAGG AGTACCTGAG CCCCCCAGAG GAGTTCCCAG AGCCTGGGGA GACCTGGCCC 
CGAACCCCTA CCATGAAGCT CAGTCCCAGC CAGGATCATG ATTCCTCCGA CTCTTCTTCC 
AAGGCACCCC CAACGTTCAA GGTCTCACTC ATGGACCAAT CGGTGAGAGA AGGTCAAGAT 
GTCATTATGA GCATCCGTGT GCAGGGAGAG CCCAAGCCTG TGGTTTCCTG GCTGAGGAAT 
CGACAGCCCG TGCGCCCAGA CCAGCGGCGC TTTGCAGAGG AGGCCGAGGG TGGGCTCTGC 
CGCTTGAGGA TCCTGGCTGC TGAACGGGGC GATGCTGGTT TCTACACATG CAAGGCGGTC 
AACGAATATG GCGCTCGGCA GTGCGAGGCC CGCCTGGAGG TCCGAGCACA CCCTGAAAGC 
CGGTCCCTGG CCGTGCTGGC CCCCCTGCAG GACGTGGACG TGGGGGCCGG GGAGATGGCG 
CTGTTTGAGT GCCTGGTGGC AGGTCCAGCT GACGTCGAGG TGGACTGGCT GTGCCGGGGC 
CGCCTGCTGC AGCCTGCGCT GCTCAAATGC AAGATGCATT TTGATGGCCG CAAGTGCAAG 
CTGCTGCTCA CCTCTGTGCA CGAGGACGAC AGTGGTGTCT ACACCTGCAA GCTGAGCACA 
GCCAAAGATG AACTGACCTG CAGTGCCCGG CTGACGGTTC GGCCGTCGCT GGCGCCTCTC 
TTCACCCGGT TGCTGGAAGA TGTGGAGGTG TTGGAAGGCC GTGCTGCCCG CTTGGATTGC 
AAGATCAGCG GTACCCCGCC TCCCTCCGTT ACCTGGACTC ATTTTGGCCA CCCCGTGAAT 
GAGGGTGACA ACTTGCGGCT TCAACAGGAT GGAGGTCTAC ACTCACTGCA CATTGCCCGG 
GTGGGCAGTG AGGACGAAGG ACTCTATGAA GTCAGTGCCA CCAACACTCA TGGCCAGGCC 
CACTGCTCCG CCCAGCTGTA TGTGGAGGAG CCTCGGACAG CTGCCTCTGG CCCCAGCTCG 
AAGCTGGAGA AGATGCCATC TATCCCTGAG GAGCCGGAGC ACGGAGATCT AGAGAGGCTG 
TCTATTCCTG ACTTCCTACG GCCACTGCAA GATCTGGAAG TGGGACTAGC TAAGGAGGCC 
ATGCTGGAGT GCCAAGTGAC CGGCCTGCCC TATCCCACCA TCAGCTGGTT CCACAATGGC 
CACCGCATTC AAAGCAGTGA CGACCGACGC ATGACACAGT ACAGGGACAT ACATCGCCTG 
GTGTTCCCAG CTGTGGGGCC TCAGCATGCT GGTGTCTATA AGAGTGTAAT CGCCAACAAG 
CTGGGCAAAG CGGCCTGCTA CGCCCATCTC TATGTCACAG ATGTGGTTCC AGGCCCTCCA 
GATGGTGCTC CGGAAGTAGT AGCTGTGACC GGAAGGATGG TCACACTATC GTGGAACCCA 
CCCAGGAGTC TGGACATGGC TATTGACCCA GATTCCCTAA CATACACTGT GCAGCACCAG 
GTGTTGGGCT CGGACCAGTG GACGGCACTG GTCACAGGCC TGCGGGAGCC TGCGTGGGCA 
GCCACAGGGC TGAAGAAGGG GATCCAGCAC ATCTTCCGGG TCCTCAGCTC CAGTGGCAAG 
AGCAGCAGCA AGCCCTCAGC ACCGTCGGAA CCCGTGCAGC TGCTAGAGCA TGGCCCACCC 
CTGGAAGAGG CGCCTGCTGT GCTGGACAAG CAGGACATTG TGTATGTGGT AGAGGGCCAG 
CCTGCCTGTG TCACCGTCAC TTTTAACCAT GTGGAGGCCC AGGTTGTCTG GAGGAGCTGC 
CGGGGAGCCC TCCTCGAGGC GCGCACAGGT GTGTATGAAC TGAGTCAGCC AGATGACGAC 
CAGTATTGTC TGCGGATCTG CCGGGTGAGC CGACGAGACC TGGGACCCCT TACTTGCAGT 
GCCCGAAACC GCCACGGCAC AAAGGCCTGC TCGGTCACCC TGGAGCTGGC AGAGGCACCT 
CGGTTTGAGT CTATCATGGA AGATGTGGAG GTGGGGCCTG GAGAAACCGC TCGCTTTGCT 
GTGGTAGTTG AGGGGAAACC ACTGCCAGAC ATCATGTGGT ACAAGGATGA GGTGCTGCTG 
GCCGAGAGCA ACCATGTGAG CTTCGTGTAT GAGGAGAACG AGTGTTCCCT AGTGCTGCTC 
AGCGCAGGAA GCCAGGATGG AGGCGTCTAT ACCTGTACCG CCCGGAACCT GGCAGGCGAA 
GTCTCCTGCA AGGCGGAGCT GTCTGTGCTT TCAGCTCAGA CAGCCATGGA GGTCGAGGGG 
GTCGGAGAGG ATGAGGAACA CCGAGGAAGA AGACTTAGCG ACTACTACGA CATCCACCAA 
GAGATTGGCA GGGGTGCCTT CTCCTACCTG CGGCGTGTAG TGGAGAGGAG CTCTGGCCTG 
GAGTTTGCAG CCAAGTTCAT CCCTAGCCAG GCTAAGCCTA AAGCATCAGC AAGACGCGAG 
GCGCGCTTGC TGGCCCGGCT CCAGCATGGC TGTGTGCTCT ACTTCCACGA AGCCTTTGAG 
AGGCGCAGGG GACTTGTCAT AGTCACGGAG CTCTGCACAG AAGAGCTTCT AGAGCGCATG 
GCCAGGAAGC CCACCGTGTG TGAGTCTGAG ACTCGGACCT ATATGCGTCA AGTGCTAGAG 
GGGATATGCT ATCTGCATCA GAGCCATGTG CTGCACCTGG ATGTCAAGCC GGAGAACCTG 
CTTGTGTGGG ATGGTGCAGG GGGTGAAGAG CAGGTGCGCA TCTGTGACTT CGGGAACGCC 
CAGGAACTCA CTCCGGGAGA GCCCCAGTAC TGTCAGTATG GCACGCCAGA GTTTGTAGCA 
CCTGAGATTG TCAACCAGAG CCCTGTGTCT GGAGTCACTG ACATCTGGCC CGTGGGCGTC 
GTTGCCTTTC TCTGTTTGAC GGGCATCTCC CCATTTGTTG GGGAGAATGA CCGGACAACA 
CTCATGAATA TCCGAAACTA CAACGTGGCA TTTGAGGAGA CTACGTTCCT GAGTCTGAGC 
AGGGAGGCCC GGGGCTTCCT CATCAAAGTG CTGGTGCAGG ATCGACTGAG ACCTACTGCA 
GAAGAGACAT TGGAACATCC TTGGTTCAAA ACAGAAGCAA AGGGTGCAGA GGTGAGCACA 
GATCACCTGA AGCTCTTCCT GTCACGGAGG AGGTGGCAGC GCTCTCAAAT CAGCTACAAG 
TGCCACCTGG TGCTGCGCCC CATTCCGGAA CTGCTACGGG CTCCCCCGGA GCGGGTGTGG 
GTGGCCATGC CTAGAAGACA ACCCCCGAGT GGGGGACTCT CATCCTCTTC AGACTCAGAA 
GAGGAAGAGT TGGAGGAACT TCCCTCAGTA CCCCGCCCCC TGCAGCCTGA GTTCTCAGGT 
TCTCGGGTAT CCCTCACCGA CATCCCCACC GAAGATGAGG CTCTGGGGAC CCCGGAAGCT 
GGGGCTGCCA CCCCCATGGA CTGGCAAGAG CAGGAAAGGA CTCCCTCTAA GGACCAGGAG 
GCCCCCAGCC CTGAGGCACT CCCCTCTCCA GGCCAGGAGT CCCCTGATGG GCCTAGCCCC 
AGGCGGCCAG AGCTCCGCAG GGGTAGCTCT GCTGAGAGTG CCCTGCCCCG AGTCGGGTCG 
CGGGAACCGG GCCGGAGCTT GCACAAGGCA GCATCCGTGG AGCTGCCGCA GCGCAGGAGC 
CCCAGCCCGG GTGCCACCCG CCTGACTCGG GGAGGCCTGG GTGAGGGCGA GTATGCCCAG 
AGGCTGCAGG CCCTGCGTCA GCGGCTGCTG AGGGGAGGCC CCGAGGATGG CAAGGTCAGC 
GGCCTCAGGG GACCCCTGCT GGAGAGCCTG GGGGGCCGTG CACGGGATCC CAGGATGGCA 
AGGGCTGCCT CCAGCGAGGC TGCCCCCCAC CACCAGCCCC CTCCGGAGTC CCGGGGTCTG 
CAAAAGAGCA GCAGCTTCTC TCAGGGCGAG GCAGAGCCCC GGGGTCGGCA CCGCCGCGCG 
GGGGCACCCC TCGAAATCCC CGTGGCCAGA CTTGGGGCTC GCAGGCTGCA GGAGTCTCCC 
TCCTTATCTG CCCTTAGCGA GACCCAGCCA CCGAGCCCTG CGAGGCCCAG CGTCCCCAAA 
CTCAGTATCA CCAAGTCCCC GGAACCTTCC GCTGTCACAT CCAGAGACTC TCCCCAGCCC 
CCGGAACCTC AGCCTGTCCC AGAAAAGGTC CCTGAGCCCA AACCAGAACC AGTCCGAGCT 
GCAAAACCAG CACAACCCCC TTTGGCTCTG CAAATGCCAA CGCAGCCTCT CACCCCCTAT 
GCCCAGATCA TGCAGTCGCT CCAGCTGTCC AGTCCCACCC TGTCCCCACA GGACCCCGCT 
GTGCCTCCAT CAGAGCCCAA GCCCCACGCG GCTGTGTTTG CCAGGGTGGC TTCCCCACCA 
CCCGGAGTCT CTGAGAAGCG CGTGCCTTCG GCCAGAACTC CCCCAGTGCT AGCCGAGAAA 
GCCCGAGTCC CCACGGTGCC CCCCAGGCCA GGCAGCAGCC TCAGCGGCAG CATCGAAAAC 
CTTGAATCAG AGGCTGTGTT CGAGGCTAAG TTCAAACGCA GCCGCGAATC GCCACTGTCG 
CGGGGCCTGA GGCTACTGAG CCGCTCGCGC TCGGAGGAGC GCGGTCCCTT CCGTGGGGCG 
GAGGACGACG GCATATACCG GCCCAGTCCG GCAGGAACGC CGCTAGAGCT GGTGCGACGG 
CCCGAGCGCT CGCGCTCCGT GCAGGACCTC AGGGTAGCCG GAGAGCCGGG CCTTGTGCGT 
CGCCTTTCGC TGTCCCTGTC GCAGAAGCTG CGGCGGACAC CGCCGGGACA GCGACACCCT 
GCCTGGGAGT CCCGCAGCGG AGACGGGGAG AGCTCCGAGG GCGGCAGCTC AGCTCGGGCA 
TCCCCAGTGC TGGCGGTACG CAGGAGACTC AGCTCCACGC TGGAGCGTCT GTCCAGCCGT 
TTGCAGCGCA GCGGCAGCAG CGAGGACTCC GGGGGCGCCT CGGGTCGCAG CACGCCGCTG 
TTTGGACGGC TGCGCAGGGC TACTTCGGAA GGGGAGAGCC TGCGACGCCT CGGTGTTCCG 
CACAATCAGC TGGGCTCTCA GACTGGTGCC ACTACACCTT CCGCTGAGTC TCTGGGCTCA 
GAGGCCAGTG GCACATCGGG TTCTTCAGCT CCTGGAGAAA GCCGAAGCCG GCACCGGTGG 
GGCCTTTCCA GGCTGCGCAA GGACAAGGGA TTATCTCAGC CAAACCTCTC CTCCAGTGTC 
CAAGAGGACT TGGGTCACCA GTATGTGCCC AGTGAGTCAG ATTTCCCCCC AGTCTTCCAC 
ATCAAACTCA AGGACCAGGT GCTGTTGGAG GGGGAAGCAG CCACCCTGCT CTGCCTACCC 
GCAGCCTGCC CAGCACCCCG CATCTCCTGG ATGAAAGATA AGCAGTCCCT GCGTTCAGAG 
CCCTCAGTGG TCATTGTGTC CTGCAAAGAT GGACGGCAGC TGCTGAGCAT ACCCCGGGCC 
GGCAAGCGGC ATGCTGGGCT CTATGAGTGC TCAGCCACCA ATGTCCTGGG CAGCATTACC 
AGCTCCTGTA CTGTGGCCGT GGCCCGCATT CCAGGGAAGC TAGCTCCTCC AGAGGTGCCC 
CAGACCTACC ATGACACAGC ACTAGTAGTG TGGAAGCCAG GAGACGGTCG GGCACCTTGC 
ACATACACTC TGGAGCGGCG GGTGGATGGG GAATCTGTCT GGCACCCTGT GAGCTCAGGC 
ATCCCCGACT GTTATTACAA TGTGACTCAG CTGCCTGTCG GCGTGACCGT GCGGTTCCGT 
GTGGCCTGTT CCAATCGTGC TGGGCAGGGG CCCTTCAGCA ACCCTTCTGA GAAGGTCTTC 
ATCCGAGGCA CTCCAGATTC TCCTGCTCAG CCAGCTGCTG CTCCCCGAGA TGCCCCTGTT 
ACCTCAGGGC CAACCAGGGC CCCACCGCCT GACTCTCCTA CCTCACTGGC CCCCACCCCC 
GCTCTTGCGC CCCCAGCCTC CCAGGCATCC ACTCTCAGCC CCTCAACTTC CTCCATGTCT 
GCCAACCAGG CCTTGTCCTC ACTCAAGGCT GTGGGTCCAC CACCCGCAAC CCCTCCACGA 
AAGCACAGGG GCCTTCTGGC CACCCAGCAA GCAGAGCCAT CCCCACCCAG TATCGTGGTC 
ACCCCAAGTG AACCCAGGTC TTTTGTCCCT GACACTGGAA CCCTGACCCC AACCTCTAGT 
CCTCAAGGGG TTAAACCAGC ACCTTCCTCA ACTTCCTTGT ATATGGTGAC TTCCTTCGTG 
TCTGCACCTC CAGCCCCCCA GGCCCCAGCC CCTGAGCCCC CTCCTGAGCC CACCAAGGTG 
ACTGTGCGGA GCCTCAGCCC AGCCAAGGAG GTGGTCAGCT CCCCAACTCC TGAGAGCACC 
ACTCTTCGGC AGGGTCCCCC TCAGAAACCC TACACCTTCC TGGAGGAGAA GGCCAGGGGC 
CGCTTTGGCG TTGTGCGGTC ATGCCGGGAG AATGCTACGG GCCGAACGTT TGTCGCCAAG 
ATCGTGCCGT ACGCCGCGGA GGGCAAGCGA CGCGTGCTGC AGGAATACGA GGTGCTTCGG 
ACGCTGCACC ACGAGCGGCT CATGTCCCTG CACGAGGCCT ACATCACCCC TCGGTACCTC 
GTGCTTATCG CTGAGAGCTG TGGCAACCGG GAACTCCTCT GTGGGCTCAG CGACAGATTC 
CGGTATTCAG AAGATGACGT GGCCACCTAT GTGGTGCAGC TGCTACAAGG CCTGGACTAC 
CTCCACGGCC ACCACGTGCT ACACCTGGAC ATCAAGCCGG ACAATCTACT GTTGGCCGCT 
GACAATGCCC TTAAAATTGT GGACTTCGGC AGTGCTCAGC CCTATAACCC TCAGGCCCTG 
AAGCCACTTG GCCACCGCAC AGGCACTCTG GAGTTCATGG CTCCTGAGAT GGTGAAGGGA 
GACCCCATTG GCTCTGCCAC GGACATCTGG GGAGCGGGTG TGCTCACCTA CATCATGCAC 
GCAACCAAAA GCAAAGTGGC ATTATCCCTG AATACTGTGC CCCTTCCAGA TGTCCTACAT 
GAATGTCTCT TAGACCTTTC AACTGCACAT CACTCTAAGG TAGAGGCAAT GAGAGAGGCA 
TCCGAGATGG CAATTCACCT TTCTTCCACA ACAGGCTTAG TGGGTACTCC CCATTCTATG 
AGCCAGACCC CCAGGAAACA GAGGCTCGGA TTGTTGGGGG TCGCTTTGAT GCCTTCCAGT 
TGTATCCTAA CACATCCCAG AGTGCCACCC TCTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SPEG (Striated Muscle Preferentially Expressed Protein Kinase) Is Essential for Cardiac Function by Regulating Junctional Membrane Complex Activity.
Circulation research120(1)110-119(2017 Jan)
Quick AP,Wang Q,Philippen LE,Barreto-Torres G,Chiang DY,Beavers D,Wang G,Khalid M,Reynolds JO,Campbell HM,Showell J,McCauley MD,Scholten A,Wehrens XH

Disruption of striated preferentially expressed gene locus leads to dilated cardiomyopathy in mice.
Circulation119(2)261-8(2009 Jan)
Liu X,Ramjiganesh T,Chen YH,Chung SW,Hall SR,Schissel SL,Padera RF Jr,Liao R,Ackerman KG,Kajstura J,Leri A,Anversa P,Yet SF,Layne MD,Perrella MA

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Speg cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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