SPEG cDNA ORF clone, Bos taurus(cattle)

The following SPEG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPEG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa16715 XM_005202851.1
Latest version!
Bos taurus SPEG complex locus (SPEG), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $104.30
$149.00
OBa01572 NM_001142485.1
Latest version!
Bos taurus SPEG complex locus (SPEG), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa216243 NM_001142485.2
Latest version!
Bos taurus SPEG complex locus (SPEG), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa245707 NM_001142485.3
Latest version!
Bos taurus striated muscle enriched protein kinase (SPEG), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa16713 XM_005202849.3
Latest version!
Bos taurus SPEG complex locus (SPEG), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa16713 XM_015459169.1
Latest version!
Bos taurus SPEG complex locus (SPEG), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa16713 XM_005202848.3
Latest version!
Bos taurus SPEG complex locus (SPEG), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa50732 XM_010802350.2
Latest version!
Bos taurus SPEG complex locus (SPEG), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa87987 XM_015459155.1
Latest version!
Bos taurus SPEG complex locus (SPEG), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa87988 XM_015459160.1
Latest version!
Bos taurus SPEG complex locus (SPEG), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa87989 XM_015459172.1
Latest version!
Bos taurus SPEG complex locus (SPEG), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa87990 XM_015459173.1
Latest version!
Bos taurus SPEG complex locus (SPEG), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa87991 XM_015459174.1
Latest version!
Bos taurus SPEG complex locus (SPEG), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa16715
    Clone ID Related Accession (Same CDS sequence) XM_005202851.1
    Accession Version XM_005202851.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 342bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product striated muscle preferentially expressed protein kinase isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103849.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    ATGAAGCTCA GTCCTGGCCA GAATCGCCGT TCCTCCGACA CTGGCTCCAA GGCGCCCCCT 
    ACTTTCAAGG TTTCACTTAT GGACCAGTCA GTAAGGGAAG GCCAAGACGT CACCATGAGC
    ATCCGTGTGC AGGGCGAACC CAAGCCGGTG GTCTCCTGGC TGAGAAACCG TCAGCCTGTG
    CGCCCAGACC AGCGGCGCTT TGCTGAGGAG GCAGAGGATG GGCTCTGCCG GCTTCGGATC
    CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC TACACCTGCA AGGCGGTTAA CGAGTATGGC
    GCTCGACAGT GTGAGGCGCG CCTGGAGGTC AGAGGCGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202908.1
    CDS230..571
    Translation

    Target ORF information:

    RefSeq Version XM_005202851.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SPEG complex locus (SPEG), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202851.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    ATGAAGCTCA GTCCTGGCCA GAATCGCCGT TCCTCCGACA CTGGCTCCAA GGCGCCCCCT 
    ACTTTCAAGG TTTCACTTAT GGACCAGTCA GTAAGGGAAG GCCAAGACGT CACCATGAGC
    ATCCGTGTGC AGGGCGAACC CAAGCCGGTG GTCTCCTGGC TGAGAAACCG TCAGCCTGTG
    CGCCCAGACC AGCGGCGCTT TGCTGAGGAG GCAGAGGATG GGCTCTGCCG GCTTCGGATC
    CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC TACACCTGCA AGGCGGTTAA CGAGTATGGC
    GCTCGACAGT GTGAGGCGCG CCTGGAGGTC AGAGGCGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01572
    Clone ID Related Accession (Same CDS sequence) NM_001142485.1
    Accession Version NM_001142485.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2009-01-03
    Organism Bos taurus(Cattle)
    Product SPEG complex locus
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03006790.1 and AAFC03021879.1. On Dec 4, 2008 this sequence version replaced XM_868492.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCGGGCA CGAGGGCACC CCCCAGCCCC 
    GGGGTGCCCC CGAAGAGGGC CAAAGTGGGG GCCGGCGGAG GGGCGGCGGC CGCGGTGGCC
    GGGGCGCCGG TCTTCCTACG GCCCCTGAAG GACGCGGCGG TGTCTGCGGG CAGCGACGTG
    CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCAGCTGGTT CCGGGATGGG
    CAGCGCTTGC CCCCGCCGGC CCCTGAGCCC AGCTGCCTGT GGCTGCGAAG CTGCGGGCCG
    CGGGATGCCG GCGTGTACAG CTGCAGGGCC CAGAACGAGC TGGGCCAGGC CTCCTGCGAG
    GCGGTTCTCA CCGTGCTGGA GGTCGGAGAC TCGGAGACAG GCGAGGATGA CATCAGCGAC
    GGGCAGGGGA CCCAGCGCCT GGAGCTTCGG GATGATGGGG CCTTCAGCAC CCCCACGGGG
    GGCTCTGATA CCCTGGTGGG CACCTCCCTG GACACGCCCC CGACCTCCGT GACAGGCACC
    TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC
    AGCAGGGCTG GCACCCGCCG CCTCCCGGGC AGCCCAAGGC AAGCACAGGC AACCGGGGCC
    GGGCCACGGC ACCTGGGGGT GGAGCCGCTC GTACGGGCAT CTCGAGCTAA TCTGGTGGGC
    GCAAGCTGGG GGTCAGAGGA TAGCCTTTCG GTGGCCAGTG ACCTGTACGG CAGCGCATTC
    AGCCTGTACA GAGGACGGGC GCTCTCGATC CACGTCAGTG TCCCTCAGAG CGGACTGCAC
    AAGGAGGAGC CTGACCTTCA GCCTCAGCCC GCCAGCGAAG CCCCCCGTCG CCCAGCCCTG
    CCGCCCCCCT CCAAATCCGC GCTGCTGCCC CCACCGTCCC CTCGGGTGGG TAAGCGGCCC
    CCGCCGGGAG CCGGCGGCCA GCCCCTGGCC ACCCCCACGC CGCCGCACCG GCGCTCTCGG
    GAGCCGGTGC CGCCCGAGGA CGCCACCGCC GAAGAAAAGC GAGGGAAAAA GTCCAAGTCG
    TCCGGGCCCT CGCTAGCGGG CACGGCGGAG TCCCGGCCCC AGACGCCCCT GAGTGAGGCC
    TCGGGCCGCC AGTCGGCGCT GGGCCGCTCC CCGAGGCTGG TGCGCGCTGG CTCCCGCATC
    CTGGACAAGC TGCAGTTCTT CGAGGAGCGC CGGCGCAGCC TGGAGCGCAG CGACTCGCCG
    CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG CGCAAGGCCC GTTCGCTGGA GCAGCCCAAG
    TCCGAGGGCG GCGGGCCGTG GGGCACGCCC AGGGCCTCGC AGGAGGAGCT GCGGTCCCCG
    GCGGGCAGCG TGGCCGAGCG GCGCCGCCTA TTCCAACAGA AGGCGGCCTC GCTGGACGAG
    CGCACGCGTC AGCGCAGCCC TGCGTCCGAC CTCGAGCTGC GCTTCGCCCA GGAGCTGGGC
    CGCATCCGCC GCTCCACGTC GCGGGAGGAG CTGGTGCGCT CTCACGAGTC CCTGCGCGCC
    ACGCTGCAGC GCGCCCCGTC CCCACGAGAG CCCGGCGAGC CGCCGCTCTT CTCCCAGCCC
    TCCACCCCCA AGACCCCGCG GGCCTTGAGC CCCGCCGCCG CCCAGCCGCC CCCTGCGAGC
    GTCGCGGAAA AGCCCGGGGA TGAGCCAGGG AGGCCCCGGG GCCGTGGGCC AGCGGGCAGG
    ACGGAGCCGG GGGAAGGCCC GCAGCAGGAG GTTAGGCGCC GGGACCAGTT CCCGCTGACC
    CGGAGCAGAG CCATCCAGGA GTGCCGGAGC CCGGTGCCGC CCCCCACCTC TGAGCCCCCC
    GAGACCAGGA CGAAAGCTCC CCCGGGTCGG AAGCGGGAGC CCCCGCCGCA GGCCGTGCGC
    TTCCTGCCCT GGGCCACGCC GGGCCAGGAG GGCGCTGCTG TGCCCCAGAC CTTGGAGAAG
    AACAGGGCGG GACCCGAGGC CGAGAAGAGG CTGCGCAGAG GGCCAGAGGA GGACGGTCCT
    TGGGGGGCCT GGGACCGCCG AGGGGCCCGC AGCCAGGGCA GAGGTCGCCG GGCGCGGCCC
    ACCTCGCCTG AGCTCGAGTC TTCGGATGAC TCCTATGTGT CTGCTGGAGA AGAGCCTCTG
    GAGGCCCCTG TGTTTGAGAT CCCCCTGCAA AATGCAGTGG TGGCCCCAGG GGTGGACGTG
    CTGCTCAAGT GTATTGTTAC CGCCAACCCC CCGCCCCAAG TGTCCTGGCA GAAGGATGGG
    TCCCCGCTGC GCAGTGATGG CCGCCTTCTC ATCCGGGCAG AAGGCGAGCG GCACACCCTG
    CTGCTCCGGG AGGCCCGGGC CGCCGATGCC GGGAGCTATG CAGCCACTGC CACCAACGAG
    CTGGGCCAGG CCCGCTGCGC TGCCACGCTG GCCGTGAGAC CTGGTGGGTC AACATCTCCT
    TTCAGCAGTC CTATCACCTC TGACGAGGAG TATCTGAGCC CCCCAGAGGA GTTCCCGGAA
    CCCGGGGAGA CCTGGCCCCG AGCCCCCACC ATGAAGCTCA GTCCTGGCCA GAATCGCCGT
    TCCTCCGACA CTGGCTCCAA GGCGCCCCCT ACTTTCAAGG TTTCACTTAT GGACCAGTCA
    GTAAGGGAAG GCCAAGACGT CACCATGAGC ATCCGTGTGC AGGGCGAACC CAAGCCGGTG
    GTCTCCTGGC TGAGAAACCG TCAGCCTGTG CGCCCAGACC AGCGGCGCTT TGCTGAGGAG
    GCAGAGGATG GGCTCTGCCG GCTTCGGATC CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC
    TACACCTGCA AGGCGGTTAA CGAGTATGGC GCTCGACAGT GTGAGGCGCG CCTGGAGGTC
    AGAGCACACC CTGAAAGCCG GTCCCTGGCC GTGCTGGCCC CTCTGCAGGA CGTGGACGTG
    GGGGCCGGGG AGATGGCGCT GTTTGAGTGC CTGGTGGCGG GTCCCACCGA CGTGGAGGTA
    GACTGGCTGT GCCGAGGCCG CCTGCTGCAG CCTGCACTAC TCAAATGCAA GATGCATTTC
    GATGGCCGCA AATGCAAGCT GCTGCTCACC TCTGTGCATG AGGACGACAG CGGCGTCTAC
    ACCTGCAAGC TCAGCACGGC CAAAGATGAA CTGACCTGCA GTGCCCGGCT GACCGTGCGA
    CCCTCGCTGG CACCCCTGTT CACGCGGCTG CTGGAGGACG TGGAAGTGCT GGAGGGCCGC
    GCTGCCCGCT TCGACTGCAA GATCAGCGGG AGCCCGCCCC CCTCCGTTAC CTGGACTCAC
    TTTGGCCACC CCGTGGAGGA GAGCGAGAAC GTGCGGCTGC GGCAGGACGG GGGCCTGCAT
    TCGCTGCACA TCGCACATGT GGGCAGTGAG GATGAGGGGC TGTACGCGGT CAGTGCTGCC
    AACACCCACG GCCAGGCCCA CTGCTCAGCC CAGCTCTACG TGGAGGAGCC TCGGACGGCT
    GCCACCGGCC CTAGCTCCAA GCTGGAGAAG ATGCCCTCCA TCCCCGAGGA GCCCGAGCAG
    GGTGAGCTGG AGCGGCTGTC CATGCCCGAC TTCCTGCGGC CGCTGCAGGA TCTGGACGTG
    GGACTGGCCA AGGAGGCCAT GCTGGAGTGC CAGGTGACCG GCCTGCCCTA CCCCACCATC
    AGCTGGTTCC ACAACGGCCA CCGCATCCAG AGCAGCGACG ACCGACGCAT GACGCAGTAC
    AGGGATGTCC ATCGCTTGGT GTTCCCTGCC GTGGGACCTC AGCATGCTGG TGTCTACAAG
    AGCGTCATTG CTAACAAGCT GGGCAAAGCC GCCTGCTATG CTCATCTCTA CGTCACAGAT
    GTGGTTCCAG GCCCCCCAGA CGGTGCCCCT CAGGTAGTGG CTGTGACCGG GAGGATGATC
    ACGCTTGCAT GGAACCCCCC GAGGAGTCTG GACATGGCTA TCGACCCAGA CGCCCTGACC
    TACACCGTGC AGCACCAGGT GCTGGGCTCA GACCAGTGGA CGGCGCTGGT CACGGGCCTG
    CGGCAGCCCG GGTGGGCAGC CACCGGATTG CGAAAGGGGG TCCAGCACGT CTTCCGGGTT
    CTCAGCACCA CCATCAAGAG CAGCAGCAAG CCCTCGCCCC CGTCCGAGCC CACGCAGCTG
    CTGGAGCGTG GCCCGCCCCT GGAGGAGGCG CCCGCTGTGC TGGACAAGCC AGACGTCGTG
    TATGTGGTGG AGGGCCAGCC CACCAGTGTC ACCGTCACCT TCAACCACGT GGAGGCCCAG
    GTTGTCTGGA GGAGCTGCCG AGGAGCCCTC CTAGAGGCCC GGCCAGGTGT GTTTGAGCTG
    AGCCAGCCAG ATGACGACCA GTACTCTCTT AGGATCTGCC GGGTGAGCCG CCGGGACCTG
    GGAGCCCTCA CTTGCACTGC CCGCAACCGC CACGGCACAC AGGCCTGCTC AGTCACCCTG
    GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC ATCATGGAGG ATGTGGAGGT AGGGGCTGGA
    GAGACTGCTC GCTTCGCCGT GGTGGTGGAC GGGAAGCCCT TGCCGGACAT CATGTGGTAC
    AAGGATGAGG CGCTGCTGAC CGAGAGCAAC CATGTGAGCT TCGTGTACGA GGAGAACGAG
    TGCTCCCTGG TGGTGCTCAG CGCGGGGGCC CAGGATGGAG GGGTCTACAC CTGCACGGCC
    CGTAACCTGG CTGGCGAAGT CTCCTGCAAA GCAGAGCTGG CTGTGCACTC AGCTCAGACA
    GCTATGGAGG TGGAAGGGAT CGGGGAAGAC GAGGAGCAGC GAGGAAGGAG GCTCAGCGAC
    TTCTATGATA TTCAGCAGGA GATCGGCAGG GGTGCCTTCT CCTACCTGCG CCGTGTAGTG
    GAGAGGAGCT CCGGACTGGA GTTTGCAGCC AAGTTCATCC CCAGCCAGGC AAAGCCAAAG
    GCATCGGCAT GGCGGGAGGC ACGGCTGCTG GCCCGGCTCC AGCATGACTG CATCCTCTAC
    TTCCATGAGG CCTTCGAGAG GCGCCGGGGA CTGGTCATCG TCACCGAGCT CTGCACAGAA
    GAGCTTCTGG AGCGAATGGC CAGGAAACCC ACCGTGTGTG AGTCTGAGAT CCGGGCCTAC
    ATGCGGCAGG TACTAGAAGG AATATGCTAC TTGCACGAGA ACCACGTGCT GCACCTGGAT
    GTCAAGCCCG AGAACCTGCT GGTGTGGGAT GGTGTTGAGG GCGAGGAGCA GGTGAGGATC
    TGTGACTTTG GGAACGCTCA AGAGCTGACA CCGGGAGAGC CCCAGTATTG CCAATACGGC
    ACACCTGAGT TCGTGGCGCC CGAGATTGTC AACCAGACCC CTGTGTCTGG AGTCACTGAC
    ATCTGGCCCG TGGGTGTCGT CGCCTTCCTC TGCCTGACCG GAATCTCCCC GTTCGTTGGG
    GAAAATGATC GGACGACATT GATGAACATC CGAAACTACA ACGTGGCCTT CGAGGAGACC
    ACGTTCCTGA GCCTGAGCAG GGAGGCCCGG GGCTTCCTCA TCAAAGTGCT GGTGCGGGAC
    CGGCTGAGGC CTACTGCAGA GGAGACCCTG GAACATCCTT GGTTTAAAGC ACAGGCAAAG
    GGTGCAGTGG TGAGCACGGA TCACCTAAAG CTATTCCTCT CCCGGCGGAG GTGGCAGCGC
    TCCCAGATCA GCTACAAGTG CCACCTGGTG CTGCGCCCCA TCCCGGAGCT GCTGCGAGCA
    CCCCCGGAGC GGGTGTGGGT GGCCGTGCCC CGAAGACCAC CCCCTAGTGG AGGGCTGTCG
    TCATCATCCG ACTCTGAAGA GGAAGAGCTG GAGGAGCTGC CCTCTGTGCC CCGACCTCTG
    CAGCCTGAGT TTTCGGGCTC CCGGGTGTCC CTCACTGACA TTCCCACTGA GGATGAGGCC
    CTCGGGGCCC TGGAGGCTGG GGCGGCCACC CCCATGGACT GGCAGGAACA AGGAAAGGCC
    CCCTCTCAGG ACCAGGAGGC CCCCAGCCCT CAGGCACTCC CCTCCCCAGG CCAGGAGTCC
    CCGTCCGGGC CCAGCCCCCG GCGGGCAGAG CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC
    CTGCCCCGGG CCGGGCCGAG GGAACCGGGC CGGGGCCTGC ACAAGGCAGC GTCCGTCGAG
    CTGCCGCAGC GCCGAAGCCC CAGCCCGGGG GCCACCCGCC TGACCCGAGG AGGCCTGGGT
    GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC CTGCGCCAGC GGCTGCTGAG GGGTGGCCCC
    GATGATGGCA AGGTCAGCGG CCTCAGGGGC CCCCTGCTGG AGAGCCTGGG GGGCCGGGCT
    CGTGACCCCC GGCTGGCGCG GGCAGCCTCC AGCGAGGCAG CACCCCACCA CCAGCACCCA
    CCTGAGGCTC GGGGCCTGCA GAAGAGCAGT AGCTTCTCGC AGGGTGAGGC AGAGCCCCGG
    GGCCGGCACC GCCGAGCAGG GGCACCCCTT GAGATCCCCG TGGCCCGGCT GGGGGCCCGC
    AAGCTGCAGG AGTCTCCCTC CTTGTCCGCC CTCAGCGAGG CCCAACCACC TAGCCCCGTG
    CGGCCCAGTG CAGCCAAGCC CAGCACCCCT AAGTCTTCAG AACCTCTTGT GGGCACCCCC
    AGTGAGGCTG CACAGCCTCC AGCACCCCAG CCTGCCCGAG AGAAGGCCCC AGAATCTACG
    CCAGAACCAG CTCGGGGCCC CAAGCCCACA CAGCCCCCCA CAGCTCTGCA AACCCTAGCG
    CCTCCTCTCA CTCCGTATGC GCAGATCATG CAGTCGCTCC AGCTGCCAGG CCAGGCGCAA
    GGCCCCCCAC AGGGCGCAGT CGTATCTCCT ACATCTCGGT CTCCAGCATC TCGGTTGGAA
    CCCAAGCCCC ACCCGGCTGT GTTCTCCAGG GTGGCCTCCC CACCTCCGGG AGCCTCCGAG
    AAGCGCCTGC TGCCGGGAGC CGGAGCAACC CCAGGGCCAG CCGAAAAAGC CCGGGTCCCG
    GCGGTCCCCC GCAGGCCAGG GAGCAGTCTC AGCAGCAGCA TCGAGAACCT GGAGTCGGAG
    GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC CGGGAGTCGC CCCTGTCGCG GGGGCTGCGG
    CTGCTGAGCC GCTCGCGGTC GGAGGAGCGC GGCCCCTTCC GCGGGGCTGA GGAGGAGGAC
    GGCATTTACC GGCCCAGCCC GGCGGGCACG CCGCTGGAGC TGGTGCGACG GCCCGAGCGC
    TCGCGCTCCG TGCAGGACCT CAGGGCGGTC GGGGAGCCAG GCCTCGTGCG CCGCCTCTCG
    CTGTCGCTGT CCCAGCGTCT GCGGCGGACC CCGCCCGCGC AGCGCCACCC GGCCTGGGAG
    CCCCGCGGCG GGGACGGCGA GAGCTCGGAG GGCGGCAGCT CGGCGCGGGG CTCCCCGGTG
    CTGACGGTGC GCCGGAGGCT CAGCTCCACG CTGGAGCGGC TGTCGAGCCG GTTGCAGCGC
    AGCGGCAGCA GCGAGGACTC GGGGGGCGCG TCGGGCCGCA GCACGCCGCT GTTCGGCCGG
    CTGCGCAGGG CCACGTCCGA GAGCGAGAGT CTGCGGCGCC TGGACCTCGC GCAAAATCAG
    CTGGCGGCCC AAGCCGGCGC CGCCACCCCT TCCGCGGAGT CCCTGGGCTC CGAGGCCAGC
    GCTACGTCGG GCTCCTCTGC TCCTGCAGAA AGTCGAAGCC GCCTGCGCTG GGGCCTCTCT
    CGGCTGCGGA AGGACAAGAG GTTGTCACAG CCTAACCTCT CTGGCAGTGT CCAGGAGGAT
    TTGGGTCACC AGTATGTGCG CAGTGAGTCA GACTTTCCCC CAGTCTTCCA CATCAAACTC
    AAGGACCAGG TGCTGCTGGA GGGAGAGGCA GCCACCCTGC TCTGCTTGCC AGCAGCCTGC
    CCTGCACCCC ACATCTCCTG GATGAAAGAC AAGCAGTCCC TGCGGTCAGA GCCCTCGGTG
    ATCATCGTGT CCTGCAAAGA TGGTCGTCAG CTACTGAGCA TCCCCCGGGC AGGCAAGCGG
    CATGCGGGGC TCTATGAGTG CTCCGCCACC AACGTCCTTG GCAGCATCAC CAGCTCCTGT
    ACCGTGGCTG TGGCTCGTAC CCCAGGAAAG CTAGCTCCTC CTGAGGTGCC CCAGACCTAC
    CAGGACACAG CACTGGTGCT CTGGAAGCCG GGAGACAGCA AGGCGCCTTG CACATACACG
    CTGGAGCGGC GGGTGGATGG GGAGTCTTCC TGGCACCCTG TGAGCTCGGG CATCCCCGAC
    TGCTACTACA ACGTGACCCA CCTACCAGTC GGCATGGCTG TGAGGTTCCG TGTGGCCTGT
    GCCAACCGTG CTGGGCAAGG GCCTTTCAGC AACCCTTCTG AGAAGGTCCT CGTCAGGGGC
    GTGCAAGATT CCTCAGCTCT GCCATCTGCT GCTCACAGAG ATGCCCCTGT TACCTCAGGG
    CCAGCCAGGG CCCCGCCTCC TGACGCTCCT ACCTCACTGG CCCCTCCCCT GAGCCCCTCC
    CCAGCTCCTG CTCCCCCAGG CCCCCAGGCA GCCCCTCTCG GCCCTTCATC TTCCCCAGCA
    CCCCCCAGCC AGGCCTTGTC CTCCCTCAAG GCTGTGGGTC CACCACCCCA AACCCCCCCA
    CGAAAGCACC GGGGCCTGCA GGCTGCCCAG CAAGTAGAAC CTACCCCACC CAGTGCCCAG
    GACTCCCCAA GTGAGCCCAA GTCTTCCGTC CCTGACACTG GGACCCCCAT CCCAGCCTCT
    GCCCCTCAAG TGGTGAAAAC AGCCTCTTCC TCAATGCCCC TGTATATGGT GACCTCCTTT
    GTGTCTGCAC CACCAGCCCC TGAGCCCCCA GCCCCCGAGC CCCCTCCTGA GCCCACCAAA
    GTGACTGTGC GGAGCCTCAG CCCAGCCAAG GAGGTGGTTG GCTCCCCTGG GGGTGATCCC
    CCCAGGGCTC CTAAGCCAGA GGGCACCACT CTTCGACAGG GCCCTCCTCA GAAACCATAC
    ACCTTCCTGG AGGAGAAGGC CAGGGGTCGC TTTGGTGTGG TGCGTGCCTG CCGGGAGAAT
    GCCACCGGGC GAACATTCGT GGCCAAGATT GTGCCATACG CGGCCGAGGG CAAGCGGCAG
    GTCCTGCAGG AGTACGAGGT GCTGAGGACC CTGCACCACG AGCGGCTTAT GGCCCTGCAC
    GAAGCCTACA TCACCCCGCG CTACCTCGTG CTCATCGCCG AGAGCTGCGG CAACCGGGAG
    CTTCTCTGTG GGCTCAGCGA CAGATTCCGG TATTCAGAGG ATGATGTGGC CACCTATGTG
    GTGCAGCTGC TACAAGGCCT GGATTACCTC CATGGCCGCC ACGTGCTGCA CCTGGACATC
    AAGCCAGACA ACCTGCTGCT GGCCCCTGAC AACGCCCTCA AGATCGTGGA CTTTGGCAGT
    GCCCAGCCCT ACAACCCCCA GGCTCTGCGG CCCCTGGGTC ACCGCACGGG CACACTGGAG
    TTCATGGCTC CTGAGATGGT AAAGGGAGAC CCCATCGGCT CTGCCACGGA CATCTGGGGA
    GCGGGTGTGC TCACTTACAT CATGCTCAGT GGACGCTCCC CATTCTATGA GCCAGACCCC
    CAGGAAACAG AGGCTCGCAT TGTGGGGGGC CGCTTTGATG CCTTCCAGCT GTATCCCAAC
    ACATCCCAGA GTGCCACCCT CTTCTTGCGA AAGGTCCTCT CAGTACATCC CTGGAGCCGG
    CCCTCCGTGC AGGACTGCCT GGCTCACCCG TGGCTGCAGG ATGCCTACCT GATGAAACTG
    CGCCGCCAGA CACTCACCTT CACCACCAAC CGGCTCAAGG AGTTCCTGGG TGAGCAGCGG
    CGCCGCAGGG CCGAGGCCGC CACCCGCCAC AAGGTGCTGC TCCGCTCTTA CCCAGGCAGC
    CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001135957.1
    CDS1..9846
    Translation

    Target ORF information:

    RefSeq Version NM_001142485.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus SPEG complex locus (SPEG), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001142485.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCGGGCA CGAGGGCACC CCCCAGCCCC 
    GGGGTGCCCC CGAAGAGGGC CAAAGTGGGG GCCGGCGGAG GGGCGGCGGC CGCGGTGGCC
    GGGGCGCCGG TCTTCCTACG GCCCCTGAAG GACGCGGCGG TGTCTGCGGG CAGCGACGTG
    CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCAGCTGGTT CCGGGATGGG
    CAGCGCTTGC CCCCGCCGGC CCCTGAGCCC AGCTGCCTGT GGCTGCGAAG CTGCGGGCCG
    CGGGATGCCG GCGTGTACAG CTGCAGGGCC CAGAACGAGC TGGGCCAGGC CTCCTGCGAG
    GCGGTTCTCA CCGTGCTGGA GGTCGGAGAC TCGGAGACAG GCGAGGATGA CATCAGCGAC
    GGGCAGGGGA CCCAGCGCCT GGAGCTTCGG GATGATGGGG CCTTCAGCAC CCCCACGGGG
    GGCTCTGATA CCCTGGTGGG CACCTCCCTG GACACGCCCC CGACCTCCGT GACAGGCACC
    TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC
    AGCAGGGCTG GCACCCGCCG CCTCCCGGGC AGCCCAAGGC AAGCACAGGC AACCGGGGCC
    GGGCCACGGC ACCTGGGGGT GGAGCCGCTC GTACGGGCAT CTCGAGCTAA TCTGGTGGGC
    GCAAGCTGGG GGTCAGAGGA TAGCCTTTCG GTGGCCAGTG ACCTGTACGG CAGCGCATTC
    AGCCTGTACA GAGGACGGGC GCTCTCGATC CACGTCAGTG TCCCTCAGAG CGGACTGCAC
    AAGGAGGAGC CTGACCTTCA GCCTCAGCCC GCCAGCGAAG CCCCCCGTCG CCCAGCCCTG
    CCGCCCCCCT CCAAATCCGC GCTGCTGCCC CCACCGTCCC CTCGGGTGGG TAAGCGGCCC
    CCGCCGGGAG CCGGCGGCCA GCCCCTGGCC ACCCCCACGC CGCCGCACCG GCGCTCTCGG
    GAGCCGGTGC CGCCCGAGGA CGCCACCGCC GAAGAAAAGC GAGGGAAAAA GTCCAAGTCG
    TCCGGGCCCT CGCTAGCGGG CACGGCGGAG TCCCGGCCCC AGACGCCCCT GAGTGAGGCC
    TCGGGCCGCC AGTCGGCGCT GGGCCGCTCC CCGAGGCTGG TGCGCGCTGG CTCCCGCATC
    CTGGACAAGC TGCAGTTCTT CGAGGAGCGC CGGCGCAGCC TGGAGCGCAG CGACTCGCCG
    CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG CGCAAGGCCC GTTCGCTGGA GCAGCCCAAG
    TCCGAGGGCG GCGGGCCGTG GGGCACGCCC AGGGCCTCGC AGGAGGAGCT GCGGTCCCCG
    GCGGGCAGCG TGGCCGAGCG GCGCCGCCTA TTCCAACAGA AGGCGGCCTC GCTGGACGAG
    CGCACGCGTC AGCGCAGCCC TGCGTCCGAC CTCGAGCTGC GCTTCGCCCA GGAGCTGGGC
    CGCATCCGCC GCTCCACGTC GCGGGAGGAG CTGGTGCGCT CTCACGAGTC CCTGCGCGCC
    ACGCTGCAGC GCGCCCCGTC CCCACGAGAG CCCGGCGAGC CGCCGCTCTT CTCCCAGCCC
    TCCACCCCCA AGACCCCGCG GGCCTTGAGC CCCGCCGCCG CCCAGCCGCC CCCTGCGAGC
    GTCGCGGAAA AGCCCGGGGA TGAGCCAGGG AGGCCCCGGG GCCGTGGGCC AGCGGGCAGG
    ACGGAGCCGG GGGAAGGCCC GCAGCAGGAG GTTAGGCGCC GGGACCAGTT CCCGCTGACC
    CGGAGCAGAG CCATCCAGGA GTGCCGGAGC CCGGTGCCGC CCCCCACCTC TGAGCCCCCC
    GAGACCAGGA CGAAAGCTCC CCCGGGTCGG AAGCGGGAGC CCCCGCCGCA GGCCGTGCGC
    TTCCTGCCCT GGGCCACGCC GGGCCAGGAG GGCGCTGCTG TGCCCCAGAC CTTGGAGAAG
    AACAGGGCGG GACCCGAGGC CGAGAAGAGG CTGCGCAGAG GGCCAGAGGA GGACGGTCCT
    TGGGGGGCCT GGGACCGCCG AGGGGCCCGC AGCCAGGGCA GAGGTCGCCG GGCGCGGCCC
    ACCTCGCCTG AGCTCGAGTC TTCGGATGAC TCCTATGTGT CTGCTGGAGA AGAGCCTCTG
    GAGGCCCCTG TGTTTGAGAT CCCCCTGCAA AATGCAGTGG TGGCCCCAGG GGTGGACGTG
    CTGCTCAAGT GTATTGTTAC CGCCAACCCC CCGCCCCAAG TGTCCTGGCA GAAGGATGGG
    TCCCCGCTGC GCAGTGATGG CCGCCTTCTC ATCCGGGCAG AAGGCGAGCG GCACACCCTG
    CTGCTCCGGG AGGCCCGGGC CGCCGATGCC GGGAGCTATG CAGCCACTGC CACCAACGAG
    CTGGGCCAGG CCCGCTGCGC TGCCACGCTG GCCGTGAGAC CTGGTGGGTC AACATCTCCT
    TTCAGCAGTC CTATCACCTC TGACGAGGAG TATCTGAGCC CCCCAGAGGA GTTCCCGGAA
    CCCGGGGAGA CCTGGCCCCG AGCCCCCACC ATGAAGCTCA GTCCTGGCCA GAATCGCCGT
    TCCTCCGACA CTGGCTCCAA GGCGCCCCCT ACTTTCAAGG TTTCACTTAT GGACCAGTCA
    GTAAGGGAAG GCCAAGACGT CACCATGAGC ATCCGTGTGC AGGGCGAACC CAAGCCGGTG
    GTCTCCTGGC TGAGAAACCG TCAGCCTGTG CGCCCAGACC AGCGGCGCTT TGCTGAGGAG
    GCAGAGGATG GGCTCTGCCG GCTTCGGATC CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC
    TACACCTGCA AGGCGGTTAA CGAGTATGGC GCTCGACAGT GTGAGGCGCG CCTGGAGGTC
    AGAGCACACC CTGAAAGCCG GTCCCTGGCC GTGCTGGCCC CTCTGCAGGA CGTGGACGTG
    GGGGCCGGGG AGATGGCGCT GTTTGAGTGC CTGGTGGCGG GTCCCACCGA CGTGGAGGTA
    GACTGGCTGT GCCGAGGCCG CCTGCTGCAG CCTGCACTAC TCAAATGCAA GATGCATTTC
    GATGGCCGCA AATGCAAGCT GCTGCTCACC TCTGTGCATG AGGACGACAG CGGCGTCTAC
    ACCTGCAAGC TCAGCACGGC CAAAGATGAA CTGACCTGCA GTGCCCGGCT GACCGTGCGA
    CCCTCGCTGG CACCCCTGTT CACGCGGCTG CTGGAGGACG TGGAAGTGCT GGAGGGCCGC
    GCTGCCCGCT TCGACTGCAA GATCAGCGGG AGCCCGCCCC CCTCCGTTAC CTGGACTCAC
    TTTGGCCACC CCGTGGAGGA GAGCGAGAAC GTGCGGCTGC GGCAGGACGG GGGCCTGCAT
    TCGCTGCACA TCGCACATGT GGGCAGTGAG GATGAGGGGC TGTACGCGGT CAGTGCTGCC
    AACACCCACG GCCAGGCCCA CTGCTCAGCC CAGCTCTACG TGGAGGAGCC TCGGACGGCT
    GCCACCGGCC CTAGCTCCAA GCTGGAGAAG ATGCCCTCCA TCCCCGAGGA GCCCGAGCAG
    GGTGAGCTGG AGCGGCTGTC CATGCCCGAC TTCCTGCGGC CGCTGCAGGA TCTGGACGTG
    GGACTGGCCA AGGAGGCCAT GCTGGAGTGC CAGGTGACCG GCCTGCCCTA CCCCACCATC
    AGCTGGTTCC ACAACGGCCA CCGCATCCAG AGCAGCGACG ACCGACGCAT GACGCAGTAC
    AGGGATGTCC ATCGCTTGGT GTTCCCTGCC GTGGGACCTC AGCATGCTGG TGTCTACAAG
    AGCGTCATTG CTAACAAGCT GGGCAAAGCC GCCTGCTATG CTCATCTCTA CGTCACAGAT
    GTGGTTCCAG GCCCCCCAGA CGGTGCCCCT CAGGTAGTGG CTGTGACCGG GAGGATGATC
    ACGCTTGCAT GGAACCCCCC GAGGAGTCTG GACATGGCTA TCGACCCAGA CGCCCTGACC
    TACACCGTGC AGCACCAGGT GCTGGGCTCA GACCAGTGGA CGGCGCTGGT CACGGGCCTG
    CGGCAGCCCG GGTGGGCAGC CACCGGATTG CGAAAGGGGG TCCAGCACGT CTTCCGGGTT
    CTCAGCACCA CCATCAAGAG CAGCAGCAAG CCCTCGCCCC CGTCCGAGCC CACGCAGCTG
    CTGGAGCGTG GCCCGCCCCT GGAGGAGGCG CCCGCTGTGC TGGACAAGCC AGACGTCGTG
    TATGTGGTGG AGGGCCAGCC CACCAGTGTC ACCGTCACCT TCAACCACGT GGAGGCCCAG
    GTTGTCTGGA GGAGCTGCCG AGGAGCCCTC CTAGAGGCCC GGCCAGGTGT GTTTGAGCTG
    AGCCAGCCAG ATGACGACCA GTACTCTCTT AGGATCTGCC GGGTGAGCCG CCGGGACCTG
    GGAGCCCTCA CTTGCACTGC CCGCAACCGC CACGGCACAC AGGCCTGCTC AGTCACCCTG
    GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC ATCATGGAGG ATGTGGAGGT AGGGGCTGGA
    GAGACTGCTC GCTTCGCCGT GGTGGTGGAC GGGAAGCCCT TGCCGGACAT CATGTGGTAC
    AAGGATGAGG CGCTGCTGAC CGAGAGCAAC CATGTGAGCT TCGTGTACGA GGAGAACGAG
    TGCTCCCTGG TGGTGCTCAG CGCGGGGGCC CAGGATGGAG GGGTCTACAC CTGCACGGCC
    CGTAACCTGG CTGGCGAAGT CTCCTGCAAA GCAGAGCTGG CTGTGCACTC AGCTCAGACA
    GCTATGGAGG TGGAAGGGAT CGGGGAAGAC GAGGAGCAGC GAGGAAGGAG GCTCAGCGAC
    TTCTATGATA TTCAGCAGGA GATCGGCAGG GGTGCCTTCT CCTACCTGCG CCGTGTAGTG
    GAGAGGAGCT CCGGACTGGA GTTTGCAGCC AAGTTCATCC CCAGCCAGGC AAAGCCAAAG
    GCATCGGCAT GGCGGGAGGC ACGGCTGCTG GCCCGGCTCC AGCATGACTG CATCCTCTAC
    TTCCATGAGG CCTTCGAGAG GCGCCGGGGA CTGGTCATCG TCACCGAGCT CTGCACAGAA
    GAGCTTCTGG AGCGAATGGC CAGGAAACCC ACCGTGTGTG AGTCTGAGAT CCGGGCCTAC
    ATGCGGCAGG TACTAGAAGG AATATGCTAC TTGCACGAGA ACCACGTGCT GCACCTGGAT
    GTCAAGCCCG AGAACCTGCT GGTGTGGGAT GGTGTTGAGG GCGAGGAGCA GGTGAGGATC
    TGTGACTTTG GGAACGCTCA AGAGCTGACA CCGGGAGAGC CCCAGTATTG CCAATACGGC
    ACACCTGAGT TCGTGGCGCC CGAGATTGTC AACCAGACCC CTGTGTCTGG AGTCACTGAC
    ATCTGGCCCG TGGGTGTCGT CGCCTTCCTC TGCCTGACCG GAATCTCCCC GTTCGTTGGG
    GAAAATGATC GGACGACATT GATGAACATC CGAAACTACA ACGTGGCCTT CGAGGAGACC
    ACGTTCCTGA GCCTGAGCAG GGAGGCCCGG GGCTTCCTCA TCAAAGTGCT GGTGCGGGAC
    CGGCTGAGGC CTACTGCAGA GGAGACCCTG GAACATCCTT GGTTTAAAGC ACAGGCAAAG
    GGTGCAGTGG TGAGCACGGA TCACCTAAAG CTATTCCTCT CCCGGCGGAG GTGGCAGCGC
    TCCCAGATCA GCTACAAGTG CCACCTGGTG CTGCGCCCCA TCCCGGAGCT GCTGCGAGCA
    CCCCCGGAGC GGGTGTGGGT GGCCGTGCCC CGAAGACCAC CCCCTAGTGG AGGGCTGTCG
    TCATCATCCG ACTCTGAAGA GGAAGAGCTG GAGGAGCTGC CCTCTGTGCC CCGACCTCTG
    CAGCCTGAGT TTTCGGGCTC CCGGGTGTCC CTCACTGACA TTCCCACTGA GGATGAGGCC
    CTCGGGGCCC TGGAGGCTGG GGCGGCCACC CCCATGGACT GGCAGGAACA AGGAAAGGCC
    CCCTCTCAGG ACCAGGAGGC CCCCAGCCCT CAGGCACTCC CCTCCCCAGG CCAGGAGTCC
    CCGTCCGGGC CCAGCCCCCG GCGGGCAGAG CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC
    CTGCCCCGGG CCGGGCCGAG GGAACCGGGC CGGGGCCTGC ACAAGGCAGC GTCCGTCGAG
    CTGCCGCAGC GCCGAAGCCC CAGCCCGGGG GCCACCCGCC TGACCCGAGG AGGCCTGGGT
    GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC CTGCGCCAGC GGCTGCTGAG GGGTGGCCCC
    GATGATGGCA AGGTCAGCGG CCTCAGGGGC CCCCTGCTGG AGAGCCTGGG GGGCCGGGCT
    CGTGACCCCC GGCTGGCGCG GGCAGCCTCC AGCGAGGCAG CACCCCACCA CCAGCACCCA
    CCTGAGGCTC GGGGCCTGCA GAAGAGCAGT AGCTTCTCGC AGGGTGAGGC AGAGCCCCGG
    GGCCGGCACC GCCGAGCAGG GGCACCCCTT GAGATCCCCG TGGCCCGGCT GGGGGCCCGC
    AAGCTGCAGG AGTCTCCCTC CTTGTCCGCC CTCAGCGAGG CCCAACCACC TAGCCCCGTG
    CGGCCCAGTG CAGCCAAGCC CAGCACCCCT AAGTCTTCAG AACCTCTTGT GGGCACCCCC
    AGTGAGGCTG CACAGCCTCC AGCACCCCAG CCTGCCCGAG AGAAGGCCCC AGAATCTACG
    CCAGAACCAG CTCGGGGCCC CAAGCCCACA CAGCCCCCCA CAGCTCTGCA AACCCTAGCG
    CCTCCTCTCA CTCCGTATGC GCAGATCATG CAGTCGCTCC AGCTGCCAGG CCAGGCGCAA
    GGCCCCCCAC AGGGCGCAGT CGTATCTCCT ACATCTCGGT CTCCAGCATC TCGGTTGGAA
    CCCAAGCCCC ACCCGGCTGT GTTCTCCAGG GTGGCCTCCC CACCTCCGGG AGCCTCCGAG
    AAGCGCCTGC TGCCGGGAGC CGGAGCAACC CCAGGGCCAG CCGAAAAAGC CCGGGTCCCG
    GCGGTCCCCC GCAGGCCAGG GAGCAGTCTC AGCAGCAGCA TCGAGAACCT GGAGTCGGAG
    GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC CGGGAGTCGC CCCTGTCGCG GGGGCTGCGG
    CTGCTGAGCC GCTCGCGGTC GGAGGAGCGC GGCCCCTTCC GCGGGGCTGA GGAGGAGGAC
    GGCATTTACC GGCCCAGCCC GGCGGGCACG CCGCTGGAGC TGGTGCGACG GCCCGAGCGC
    TCGCGCTCCG TGCAGGACCT CAGGGCGGTC GGGGAGCCAG GCCTCGTGCG CCGCCTCTCG
    CTGTCGCTGT CCCAGCGTCT GCGGCGGACC CCGCCCGCGC AGCGCCACCC GGCCTGGGAG
    CCCCGCGGCG GGGACGGCGA GAGCTCGGAG GGCGGCAGCT CGGCGCGGGG CTCCCCGGTG
    CTGACGGTGC GCCGGAGGCT CAGCTCCACG CTGGAGCGGC TGTCGAGCCG GTTGCAGCGC
    AGCGGCAGCA GCGAGGACTC GGGGGGCGCG TCGGGCCGCA GCACGCCGCT GTTCGGCCGG
    CTGCGCAGGG CCACGTCCGA GAGCGAGAGT CTGCGGCGCC TGGACCTCGC GCAAAATCAG
    CTGGCGGCCC AAGCCGGCGC CGCCACCCCT TCCGCGGAGT CCCTGGGCTC CGAGGCCAGC
    GCTACGTCGG GCTCCTCTGC TCCTGCAGAA AGTCGAAGCC GCCTGCGCTG GGGCCTCTCT
    CGGCTGCGGA AGGACAAGAG GTTGTCACAG CCTAACCTCT CTGGCAGTGT CCAGGAGGAT
    TTGGGTCACC AGTATGTGCG CAGTGAGTCA GACTTTCCCC CAGTCTTCCA CATCAAACTC
    AAGGACCAGG TGCTGCTGGA GGGAGAGGCA GCCACCCTGC TCTGCTTGCC AGCAGCCTGC
    CCTGCACCCC ACATCTCCTG GATGAAAGAC AAGCAGTCCC TGCGGTCAGA GCCCTCGGTG
    ATCATCGTGT CCTGCAAAGA TGGTCGTCAG CTACTGAGCA TCCCCCGGGC AGGCAAGCGG
    CATGCGGGGC TCTATGAGTG CTCCGCCACC AACGTCCTTG GCAGCATCAC CAGCTCCTGT
    ACCGTGGCTG TGGCTCGTAC CCCAGGAAAG CTAGCTCCTC CTGAGGTGCC CCAGACCTAC
    CAGGACACAG CACTGGTGCT CTGGAAGCCG GGAGACAGCA AGGCGCCTTG CACATACACG
    CTGGAGCGGC GGGTGGATGG GGAGTCTTCC TGGCACCCTG TGAGCTCGGG CATCCCCGAC
    TGCTACTACA ACGTGACCCA CCTACCAGTC GGCATGGCTG TGAGGTTCCG TGTGGCCTGT
    GCCAACCGTG CTGGGCAAGG GCCTTTCAGC AACCCTTCTG AGAAGGTCCT CGTCAGGGGC
    GTGCAAGATT CCTCAGCTCT GCCATCTGCT GCTCACAGAG ATGCCCCTGT TACCTCAGGG
    CCAGCCAGGG CCCCGCCTCC TGACGCTCCT ACCTCACTGG CCCCTCCCCT GAGCCCCTCC
    CCAGCTCCTG CTCCCCCAGG CCCCCAGGCA GCCCCTCTCG GCCCTTCATC TTCCCCAGCA
    CCCCCCAGCC AGGCCTTGTC CTCCCTCAAG GCTGTGGGTC CACCACCCCA AACCCCCCCA
    CGAAAGCACC GGGGCCTGCA GGCTGCCCAG CAAGTAGAAC CTACCCCACC CAGTGCCCAG
    GACTCCCCAA GTGAGCCCAA GTCTTCCGTC CCTGACACTG GGACCCCCAT CCCAGCCTCT
    GCCCCTCAAG TGGTGAAAAC AGCCTCTTCC TCAATGCCCC TGTATATGGT GACCTCCTTT
    GTGTCTGCAC CACCAGCCCC TGAGCCCCCA GCCCCCGAGC CCCCTCCTGA GCCCACCAAA
    GTGACTGTGC GGAGCCTCAG CCCAGCCAAG GAGGTGGTTG GCTCCCCTGG GGGTGATCCC
    CCCAGGGCTC CTAAGCCAGA GGGCACCACT CTTCGACAGG GCCCTCCTCA GAAACCATAC
    ACCTTCCTGG AGGAGAAGGC CAGGGGTCGC TTTGGTGTGG TGCGTGCCTG CCGGGAGAAT
    GCCACCGGGC GAACATTCGT GGCCAAGATT GTGCCATACG CGGCCGAGGG CAAGCGGCAG
    GTCCTGCAGG AGTACGAGGT GCTGAGGACC CTGCACCACG AGCGGCTTAT GGCCCTGCAC
    GAAGCCTACA TCACCCCGCG CTACCTCGTG CTCATCGCCG AGAGCTGCGG CAACCGGGAG
    CTTCTCTGTG GGCTCAGCGA CAGATTCCGG TATTCAGAGG ATGATGTGGC CACCTATGTG
    GTGCAGCTGC TACAAGGCCT GGATTACCTC CATGGCCGCC ACGTGCTGCA CCTGGACATC
    AAGCCAGACA ACCTGCTGCT GGCCCCTGAC AACGCCCTCA AGATCGTGGA CTTTGGCAGT
    GCCCAGCCCT ACAACCCCCA GGCTCTGCGG CCCCTGGGTC ACCGCACGGG CACACTGGAG
    TTCATGGCTC CTGAGATGGT AAAGGGAGAC CCCATCGGCT CTGCCACGGA CATCTGGGGA
    GCGGGTGTGC TCACTTACAT CATGCTCAGT GGACGCTCCC CATTCTATGA GCCAGACCCC
    CAGGAAACAG AGGCTCGCAT TGTGGGGGGC CGCTTTGATG CCTTCCAGCT GTATCCCAAC
    ACATCCCAGA GTGCCACCCT CTTCTTGCGA AAGGTCCTCT CAGTACATCC CTGGAGCCGG
    CCCTCCGTGC AGGACTGCCT GGCTCACCCG TGGCTGCAGG ATGCCTACCT GATGAAACTG
    CGCCGCCAGA CACTCACCTT CACCACCAAC CGGCTCAAGG AGTTCCTGGG TGAGCAGCGG
    CGCCGCAGGG CCGAGGCCGC CACCCGCCAC AAGGTGCTGC TCCGCTCTTA CCCAGGCAGC
    CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216243
    Clone ID Related Accession (Same CDS sequence) NM_001142485.2
    Accession Version NM_001142485.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product striated muscle preferentially expressed protein kinase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02005944.1 and DAAA02005945.1. On Jun 30, 2017 this sequence version replaced NM_001142485.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145420 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCGGGCA CGAGGGCACC CCCCAGCCCC 
    GGGGTGCCCC CGAAGAGGGC CAAAGTGGGG GCCGGCGGAG GGGCGGCGGC CGCGGTGGCC
    GGGGCGCCGG TCTTCCTACG GCCCCTGAAG GACGCGGCGG TGTCTGCGGG CAGCGACGTG
    CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCAGCTGGTT CCGGGATGGG
    CAGCGCTTGC CCCCGCCGGC CCCTGAGCCC AGCTGCCTGT GGCTGCGAAG CTGCGGGCCG
    CGGGATGCCG GCGTGTACAG CTGCAGGGCC CAGAACGAGC TGGGCCAGGC CTCCTGCGAG
    GCGGTTCTCA CCGTGCTGGA GGTCGGAGAC TCGGAGACAG GCGAGGATGA CATCAGCGAC
    GGGCAGGGGA CCCAGCGCCT GGAGCTTCGG GATGATGGGG CCTTCAGCAC CCCCACGGGG
    GGCTCTGATA CCCTGGTGGG CACCTCCCTG GACACGCCCC CGACCTCCGT GACAGGCACC
    TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC
    AGCAGGGCTG GCACCCGCCG CCTCCCGGGC AGCCCAAGGC AAGCACAGGC AACCGGGGCC
    GGGCCACGGC ACCTGGGGGT GGAGCCGCTC GTACGGGCAT CTCGAGCTAA TCTGGTGGGC
    GCAAGCTGGG GGTCAGAGGA TAGCCTTTCG GTGGCCAGTG ACCTGTACGG CAGCGCATTC
    AGCCTGTACA GAGGACGGGC GCTCTCGATC CACGTCAGTG TCCCTCAGAG CGGACTGCAC
    AAGGAGGAGC CTGACCTTCA GCCTCAGCCC GCCAGCGAAG CCCCCCGTCG CCCAGCCCTG
    CCGCCCCCCT CCAAATCCGC GCTGCTGCCC CCACCGTCCC CTCGGGTGGG TAAGCGGCCC
    CCGCCGGGAG CCGGCGGCCA GCCCCTGGCC ACCCCCACGC CGCCGCACCG GCGCTCTCGG
    GAGCCGGTGC CGCCCGAGGA CGCCACCGCC GAAGAAAAGC GAGGGAAAAA GTCCAAGTCG
    TCCGGGCCCT CGCTAGCGGG CACGGCGGAG TCCCGGCCCC AGACGCCCCT GAGTGAGGCC
    TCGGGCCGCC AGTCGGCGCT GGGCCGCTCC CCGAGGCTGG TGCGCGCTGG CTCCCGCATC
    CTGGACAAGC TGCAGTTCTT CGAGGAGCGC CGGCGCAGCC TGGAGCGCAG CGACTCGCCG
    CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG CGCAAGGCCC GTTCGCTGGA GCAGCCCAAG
    TCCGAGGGCG GCGGGCCGTG GGGCACGCCC AGGGCCTCGC AGGAGGAGCT GCGGTCCCCG
    GCGGGCAGCG TGGCCGAGCG GCGCCGCCTA TTCCAACAGA AGGCGGCCTC GCTGGACGAG
    CGCACGCGTC AGCGCAGCCC TGCGTCCGAC CTCGAGCTGC GCTTCGCCCA GGAGCTGGGC
    CGCATCCGCC GCTCCACGTC GCGGGAGGAG CTGGTGCGCT CTCACGAGTC CCTGCGCGCC
    ACGCTGCAGC GCGCCCCGTC CCCCCGAGAG CCCGGCGAGC CGCCGCTCTT CTCCCAGCCC
    TCCACCCCCA AGACCCCGCG GGCCTTGAGC CCCGCCGCCG CCCAGCCGCC CCCTGCGAGC
    GTCGCGGAAA AGCCCGGGGA TGAGCCAGGG AGGCCCCGGG GCCGTGGGCC AGCGGGCAGG
    ACGGAGCCGG GGGAAGGCCC GCAGCAGGAG GTTAGGCGCC GGGACCAGTT CCCGCTGACC
    CGGAGCAGAG CCATCCAGGA GTGCCGGAGC CCGGTGCCGC CCCCCACCTC TGAGCCCCCC
    GAGACCAGGA CGAAAGCTCC CCCGGGTCGG AAGCGGGAGC CCCCGCCGCA GGCCGTGCGC
    TTCCTGCCCT GGGCCACGCC GGGCCAGGAG GGCGCTGCTG TGCCCCAGAC CTTGGAGAAG
    AACAGGGCGG GACCCGAGGC CGAGAAGAGG CTGCGCAGAG GGCCAGAGGA GGACGGTCCT
    TGGGGGGCCT GGGACCGCCG AGGGGCCCGC AGCCAGGGCA GAGGTCGCCG GGCGCGGCCC
    ACCTCGCCTG AGCTCGAGTC TTCGGATGAC TCCTATGTGT CTGCTGGAGA AGAGCCTCTG
    GAGGCCCCTG TGTTTGAGAT CCCCCTGCAA AATGCAGTGG TGGCCCCAGG GGTGGACGTG
    CTGCTCAAGT GTATTGTTAC CGCCAACCCC CCGCCCCAAG TGTCCTGGCA GAAGGATGGG
    TCCCCGCTGC GCAGTGATGG CCGCCTTCTC ATCCGGGCAG AAGGCGAGCG GCACACCCTG
    CTGCTCCGGG AGGCCCGGGC CGCCGATGCC GGGAGCTATG CAGCCACTGC CACCAACGAG
    CTGGGCCAGG CCCGCTGCGC TGCCACGCTG GCCGTGAGAC CTGGTGGGTC AACATCTCCT
    TTCAGCAGTC CTATCACCTC TGACGAGGAG TATCTGAGCC CCCCAGAGGA GTTCCCGGAA
    CCCGGGGAGA CCTGGCCCCG AGCCCCCACC ATGAAGCTCA GTCCTGGCCA GAATCGCCGT
    TCCTCCGACA CTGGCTCCAA GGCGCCCCCT ACTTTCAAGG TTTCACTTAT GGACCAGTCA
    GTAAGGGAAG GCCAAGACGT CACCATGAGC ATCCGTGTGC AGGGCGAACC CAAGCCGGTG
    GTCTCCTGGC TGAGAAACCG TCAGCCTGTG CGCCCAGACC AGCGGCGCTT TGCTGAGGAG
    GCAGAGGATG GGCTCTGCCG GCTTCGGATC CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC
    TACACCTGCA AGGCGGTTAA CGAGTATGGC GCTCGACAGT GTGAGGCGCG CCTGGAGGTC
    AGAGCACACC CTGAAAGCCG GTCCCTGGCC GTGCTGGCCC CTCTGCAGGA CGTGGACGTG
    GGGGCCGGGG AGATGGCGCT GTTTGAGTGC CTGGTGGCGG GTCCCACCGA CGTGGAGGTA
    GACTGGCTGT GCCGAGGCCG CCTGCTGCAG CCTGCACTAC TCAAATGCAA GATGCATTTC
    GATGGCCGCA AATGCAAGCT GCTGCTCACC TCTGTGCATG AGGACGACAG CGGCGTCTAC
    ACCTGCAAGC TCAGCACGGC CAAAGATGAA CTGACCTGCA GTGCCCGGCT GACCGTGCGA
    CCCTCGCTGG CACCCCTGTT CACGCGGCTG CTGGAGGACG TGGAAGTGCT GGAGGGCCGC
    GCTGCCCGCT TCGACTGCAA GATCAGCGGG AGCCCGCCCC CCTCCGTTAC CTGGACTCAC
    TTTGGCCACC CCGTGGAGGA GAGCGAGAAC GTGCGGCTGC GGCAGGACGG GGGCCTGCAT
    TCGCTGCACA TCGCACATGT GGGCAGTGAG GATGAGGGGC TGTACGCGGT CAGTGCTGCC
    AACACCCACG GCCAGGCCCA CTGCTCAGCC CAGCTCTACG TGGAGGAGCC TCGGACGGCT
    GCCACCGGCC CTAGCTCCAA GCTGGAGAAG ATGCCCTCCA TCCCCGAGGA GCCCGAGCAG
    GGTGAGCTGG AGCGGCTGTC CATGCCCGAC TTCCTGCGGC CGCTGCAGGA TCTGGACGTG
    GGACTGGCCA AGGAGGCCAT GCTGGAGTGC CAGGTGACCG GCCTGCCCTA CCCCACCATC
    AGCTGGTTCC ACAACGGCCA CCGCATCCAG AGCAGCGACG ACCGACGCAT GACGCAGTAC
    AGGGATGTCC ATCGCTTGGT GTTCCCTGCC GTGGGACCTC AGCATGCTGG TGTCTACAAG
    AGCGTCATTG CTAACAAGCT GGGCAAAGCC GCCTGCTATG CTCATCTCTA CGTCACAGAT
    GTGGTTCCAG GCCCCCCAGA CGGTGCCCCT CAGGTAGTGG CTGTGACCGG GAGGATGATC
    ACGCTTGCAT GGAACCCCCC GAGGAGTCTG GACATGGCTA TCGACCCAGA CGCCCTGACC
    TACACCGTGC AGCACCAGGT GCTGGGCTCA GACCAGTGGA CGGCGCTGGT CACGGGCCTG
    CGGCAGCCCG GGTGGGCAGC CACCGGATTG CGAAAGGGGG TCCAGCACGT CTTCCGGGTT
    CTCAGCACCA CCATCAAGAG CAGCAGCAAG CCCTCGCCCC CGTCCGAGCC CACGCAGCTG
    CTGGAGCGTG GCCCGCCCCT GGAGGAGGCG CCCGCTGTGC TGGACAAGCC AGACGTCGTG
    TATGTGGTGG AGGGCCAGCC CACCAGTGTC ACCGTCACCT TCAACCACGT GGAGGCCCAG
    GTTGTCTGGA GGAGCTGCCG AGGAGCCCTC CTAGAGGCCC GGCCAGGTGT GTTTGAGCTG
    AGCCAGCCAG ATGACGACCA GTACTCTCTT AGGATCTGCC GGGTGAGCCG CCGGGACCTG
    GGAGCCCTCA CTTGCACTGC CCGCAACCGC CACGGCACAC AGGCCTGCTC AGTCACCCTG
    GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC ATCATGGAGG ATGTGGAGGT AGGGGCTGGA
    GAGACTGCTC GCTTCGCCGT GGTGGTGGAC GGGAAGCCCT TGCCGGACAT CATGTGGTAC
    AAGGATGAGG CGCTGCTGAC CGAGAGCAAC CATGTGAGCT TCGTGTACGA GGAGAACGAG
    TGCTCCCTGG TGGTGCTCAG CGCGGGGGCC CAGGATGGAG GGGTCTACAC CTGCACGGCC
    CGTAACCTGG CTGGCGAAGT CTCCTGCAAA GCAGAGCTGG CTGTGCACTC AGCTCAGACA
    GCTATGGAGG TGGAAGGGAT CGGGGAAGAC GAGGAGCAGC GAGGAAGGAG GCTCAGCGAC
    TTCTATGATA TTCAGCAGGA GATCGGCAGG GGTGCCTTCT CCTACCTGCG CCGTGTAGTG
    GAGAGGAGCT CCGGACTGGA GTTTGCAGCC AAGTTCATCC CCAGCCAGGC AAAGCCAAAG
    GCATCGGCAT GGCGGGAGGC ACGGCTGCTG GCCCGGCTCC AGCATGACTG CATCCTCTAC
    TTCCATGAGG CCTTCGAGAG GCGCCGGGGA CTGGTCATCG TCACCGAGCT CTGCACAGAA
    GAGCTTCTGG AGCGAATGGC CAGGAAACCC ACCGTGTGTG AGTCTGAGAT CCGGGCCTAC
    ATGCGGCAGG TACTAGAAGG AATATGCTAC TTGCACGAGA ACCACGTGCT GCACCTGGAT
    GTCAAGCCCG AGAACCTGCT GGTGTGGGAT GGTGTTGAGG GCGAGGAGCA GGTGAGGATC
    TGTGACTTTG GGAACGCTCA AGAGCTGACA CCGGGAGAGC CCCAGTATTG CCAATACGGC
    ACACCTGAGT TCGTGGCGCC CGAGATTGTC AACCAGACCC CTGTGTCTGG AGTCACTGAC
    ATCTGGCCCG TGGGTGTCGT CGCCTTCCTC TGCCTGACCG GAATCTCCCC GTTCGTTGGG
    GAAAATGATC GGACGACATT GATGAACATC CGAAACTACA ACGTGGCCTT CGAGGAGACC
    ACGTTCCTGA GCCTGAGCAG GGAGGCCCGG GGCTTCCTCA TCAAAGTGCT GGTGCGGGAC
    CGGCTGAGGC CTACTGCAGA GGAGACCCTG GAACATCCTT GGTTTAAAGC ACAGGCAAAG
    GGTGCAGTGG TGAGCACGGA TCACCTAAAG CTATTCCTCT CCCGGCGGAG GTGGCAGCGC
    TCCCAGATCA GCTACAAGTG CCACCTGGTG CTGCGCCCCA TCCCGGAGCT GCTGCGAGCA
    CCCCCGGAGC GGGTGTGGGT GGCCGTGCCC CGAAGACCAC CCCCTAGTGG AGGGCTGTCG
    TCATCATCCG ACTCTGAAGA GGAAGAGCTG GAGGAGCTGC CCTCTGTGCC CCGACCTCTG
    CAGCCTGAGT TTTCGGGCTC CCGGGTGTCC CTCACTGACA TTCCCACTGA GGATGAGGCC
    CTCGGGGCCC TGGAGGCTGG GGCGGCCACC CCCATGGACT GGCAAGAACA AGGAAAGGCC
    CCCTCTCAGG ACCAGGAGGC CCCCAGCCCT CAGGCACTCC CCTCCCCAGG CCAGGAGTCC
    CCGTCCGGGC CCAGCCCCCG GCGGGCAGAG CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC
    CTGCCCCGGG CCGGGCCGAG GGAACCGGGC CGGGGCCTGC ACAAGGCAGC GTCCGTCGAG
    CTGCCGCAGC GCCGAAGCCC CAGCCCGGGG GCCACCCGCC TGACCCGAGG AGGCCTGGGT
    GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC CTGCGCCAGC GGCTGCTGAG GGGTGGCCCC
    GATGATGGCA AGGTCAGCGG CCTCAGGGGC CCCCTGCTGG AGAGCCTGGG GGGCCGGGCT
    CGTGACCCCC GGCTGGCGCG GGCAGCCTCC AGCGAGGCAG CACCCCATCA CCAGCACCCA
    CCTGAGGCTC GGGGCCTGCA GAAGAGCAGT AGCTTCTCGC AGGGTGAGGC AGAGCCCCGG
    GGCCGGCACC GCCGAGCAGG GGCACCCCTT GAGATCCCCG TGGCCCGGCT GGGGGCCCGC
    AAGCTGCAGG AGTCTCCCTC CTTGTCCGCC CTCAGCGAGG CCCAACCACC TAGCCCCGTG
    CGGCCCAGTG CAGCCAAGCC CAGCACCCCT AAGTCTTCAG AACCTCTTGT GGGCACCCCC
    AGTGAGGCTG CACAGCCTCC AGCACCCCAG CCTGCCCGAG AGAAGGCCCC AGAATCTACG
    CCAGAACCAG CTCGGGGCCC CAAGCCCACA CAGCCCCCCA CAGCTCTGCA AACCCTAGCG
    CCTCCTCTCA CTCCGTATGC GCAGATCATG CAGTCGCTCC AGCTGCCAGG CCAGGCGCAA
    GGCCCCCCAC AGGGCGCAGT CGTATCTCCT ACATCTCGGT CTCCAGCATC TCGGTTGGAA
    CCCAAGCCCC ACCCGGCTGT GTTCTCCAGG GTGGCCTCCC CACCTCCAGG AGCCTCCGAG
    AAGCGCCTGC TGCCGGGAGC CGGAGCAACC CCAGGGCCAG CCGAAAAAGC CCGGGTCCCG
    GCGGTCCCCC GCAGGCCAGG GAGCAGTCTC AGCAGCAGCA TCGAGAACCT GGAGTCGGAG
    GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC CGGGAGTCGC CCCTGTCGCG GGGGCTGCGG
    CTGCTGAGCC GCTCGCGGTC GGAGGAGCGC GGCCCCTTCC GCGGGGCTGA GGAGGAGGAC
    GGCATTTACC GGCCCAGCCC GGCGGGCACG CCGCTGGAGC TGGTGCGACG GCCCGAGCGC
    TCGCGCTCCG TGCAGGACCT CAGGGCGGTC GGGGAGCCAG GCCTCGTGCG CCGCCTCTCG
    CTGTCGCTGT CCCAGCGTCT GCGGCGGACC CCGCCCGCGC AGCGCCACCC GGCCTGGGAG
    CCCCGCGGCG GGGACGGCGA GAGCTCGGAG GGCGGCAGCT CGGCGCGGGG CTCCCCGGTG
    CTGACGGTGC GCCGGAGGCT CAGCTCCACG CTGGAGCGGC TGTCGAGCCG GTTGCAGCGC
    AGCGGCAGCA GCGAGGACTC GGGGGGCGCG TCGGGCCGCA GCACGCCGCT GTTCGGCCGG
    CTGCGCAGGG CCACGTCCGA GAGCGAGAGT CTGCGGCGCC TGGACCTCGC GCAAAATCAG
    CTGGCGGCCC AAGCCGGCGC CGCCACCCCT TCCGCGGAGT CCCTGGGCTC CGAGGCCAGC
    GCTACGTCGG GCTCCTCTGC TCCTGCAGAA AGTCGAAGCC GCCTGCGCTG GGGCCTCTCT
    CGGCTGCGGA AGGACAAGAG GTTGTCACAG CCTAACCTCT CTGGCAGTGT CCAGGAGGAT
    TTGGGTCACC AGTATGTGCG CAGTGAGTCA GACTTTCCCC CAGTCTTCCA CATCAAACTC
    AAGGACCAGG TGCTGCTGGA GGGAGAGGCA GCCACCCTGC TCTGCTTGCC GGCAGCCTGC
    CCTGCACCCC ACATCTCCTG GATGAAAGAC AAGCAGTCCC TGCGGTCAGA GCCCTCGGTG
    ATCATCGTGT CCTGCAAAGA TGGTCGTCAG CTACTGAGCA TCCCCCGGGC AGGCAAGCGG
    CATGCGGGGC TCTATGAGTG CTCCGCCACC AACGTCCTTG GCAGCATCAC CAGCTCCTGT
    ACCGTGGCTG TGGCTCGTAC CCCAGGAAAG CTAGCTCCTC CTGAGGTGCC CCAGACCTAC
    CAGGACACAG CACTGGTGCT CTGGAAGCCG GGAGACAGCA AGGCGCCTTG CACATACACG
    CTGGAGCGGC GGGTGGATGG GGAGTCTTCC TGGCACCCTG TGAGCTCGGG CATCCCCGAC
    TGCTACTACA ACGTGACCCA CCTACCAGTC GGCATGGCTG TGAGGTTCCG TGTGGCCTGT
    GCCAACCGTG CTGGGCAAGG GCCTTTCAGC AACCCTTCTG AGAAGGTCCT CGTCAGGGGC
    GTGCAAGATT CCTCAGCTCT GCCATCTGCT GCTCACAGAG ATGCCCCTGT TACCTCAGGG
    CCAGCCAGGG CCCCGCCTCC TGACGCTCCT ACCTCACTGG CCCCTCCCCT GAGCCCCTCC
    CCAGCTCCTG CTCCCCCAGG CCCCCAGGCA GCCCCTCTCG GCCCTTCATC TTCCCCAGCA
    CCCCCCAGCC AGGCCTTGTC CTCCCTCAAG GCTGTGGGTC CACCACCCCA AACCCCCCCA
    CGAAAGCACC GGGGCCTGCA GGCTGCCCAG CAAGTAGAAC CTACCCCACC CAGTGCCCAG
    GACTCCCCAA GTGAGCCCAA GTCTTCCGTC CCTGACACTG GGACCCCCAT CCCAGCCTCT
    GCCCCTCAAG TGGTGAAAAC AGCCTCTTCC TCAATGCCCC TGTATATGGT GACCTCCTTT
    GTGTCTGCAC CACCAGCCCC TGAGCCCCCA GCCCCCGAGC CCCCTCCTGA GCCCACCAAA
    GTGACTGTGC GGAGCCTCAG CCCAGCCAAG GAGGTGGTTG GCTCCCCTGG GGGTGGTCCC
    CCCAGGGCTC CTAAGCCAGA GGGCACCACT CTTCGACAGG GCCCTCCTCA GAAACCATAC
    ACCTTCCTGG AGGAGAAGGC CAGGGGTCGC TTTGGTGTGG TGCGTGCCTG CCGGGAGAAT
    GCCACCGGGC GAACATTCGT GGCCAAGATT GTGCCATACG CGGCCGAGGG CAAGCGGCAG
    GTCCTGCAGG AGTACGAGGT GCTGAGGACC CTGCACCACG AGCGGCTTAT GGCCCTGCAC
    GAAGCCTACA TCACCCCGCG CTACCTCGTG CTCATCGCCG AGAGCTGCGG CAACCGGGAG
    CTTCTCTGTG GGCTCAGCGA CAGATTCCGG TATTCAGAGG ATGATGTGGC CACCTATGTG
    GTGCAGCTGC TACAAGGCCT GGATTACCTC CATGGCCGCC ACGTGCTGCA CCTGGACATC
    AAGCCAGACA ACCTGCTGCT GGCCCCTGAC AACGCCCTCA AGATCGTGGA CTTTGGCAGT
    GCCCAGCCCT ACAACCCCCA GGCTCTGCGG CCCCTGGGTC ACCGCACGGG CACACTGGAG
    TTCATGGCTC CTGAGATGGT AAAGGGAGAC CCCATCGGCT CTGCCACGGA CATCTGGGGA
    GCGGGTGTGC TCACTTACAT CATGCTCAGT GGACGCTCCC CATTCTATGA GCCAGACCCC
    CAGGAAACAG AGGCTCGCAT TGTGGGGGGC CGCTTTGATG CCTTCCAGCT GTATCCCAAC
    ACATCCCAGA GTGCCACCCT CTTCTTGCGA AAGGTCCTCT CAGTACATCC CTGGAGCCGG
    CCCTCCGTGC AGGACTGCCT GGCTCACCCG TGGCTGCAGG ATGCCTACCT GATGAAACTG
    CGCCGCCAGA CACTCACCTT CACCACCAAC CGGCTCAAGG AGTTCCTGGG TGAGCAGCGG
    CGCCGCAGGG CCGAGGCCGC CACCCGCCAC AAGGTGCTGC TCCGCTCTTA CCCAGGCAGC
    CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001135957.2
    CDS1..9846
    Translation

    Target ORF information:

    RefSeq Version NM_001142485.2
    Organism Bos taurus(cattle)
    Definition Bos taurus SPEG complex locus (SPEG), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001142485.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCGGGCA CGAGGGCACC CCCCAGCCCC 
    GGGGTGCCCC CGAAGAGGGC CAAAGTGGGG GCCGGCGGAG GGGCGGCGGC CGCGGTGGCC
    GGGGCGCCGG TCTTCCTACG GCCCCTGAAG GACGCGGCGG TGTCTGCGGG CAGCGACGTG
    CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCAGCTGGTT CCGGGATGGG
    CAGCGCTTGC CCCCGCCGGC CCCTGAGCCC AGCTGCCTGT GGCTGCGAAG CTGCGGGCCG
    CGGGATGCCG GCGTGTACAG CTGCAGGGCC CAGAACGAGC TGGGCCAGGC CTCCTGCGAG
    GCGGTTCTCA CCGTGCTGGA GGTCGGAGAC TCGGAGACAG GCGAGGATGA CATCAGCGAC
    GGGCAGGGGA CCCAGCGCCT GGAGCTTCGG GATGATGGGG CCTTCAGCAC CCCCACGGGG
    GGCTCTGATA CCCTGGTGGG CACCTCCCTG GACACGCCCC CGACCTCCGT GACAGGCACC
    TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC
    AGCAGGGCTG GCACCCGCCG CCTCCCGGGC AGCCCAAGGC AAGCACAGGC AACCGGGGCC
    GGGCCACGGC ACCTGGGGGT GGAGCCGCTC GTACGGGCAT CTCGAGCTAA TCTGGTGGGC
    GCAAGCTGGG GGTCAGAGGA TAGCCTTTCG GTGGCCAGTG ACCTGTACGG CAGCGCATTC
    AGCCTGTACA GAGGACGGGC GCTCTCGATC CACGTCAGTG TCCCTCAGAG CGGACTGCAC
    AAGGAGGAGC CTGACCTTCA GCCTCAGCCC GCCAGCGAAG CCCCCCGTCG CCCAGCCCTG
    CCGCCCCCCT CCAAATCCGC GCTGCTGCCC CCACCGTCCC CTCGGGTGGG TAAGCGGCCC
    CCGCCGGGAG CCGGCGGCCA GCCCCTGGCC ACCCCCACGC CGCCGCACCG GCGCTCTCGG
    GAGCCGGTGC CGCCCGAGGA CGCCACCGCC GAAGAAAAGC GAGGGAAAAA GTCCAAGTCG
    TCCGGGCCCT CGCTAGCGGG CACGGCGGAG TCCCGGCCCC AGACGCCCCT GAGTGAGGCC
    TCGGGCCGCC AGTCGGCGCT GGGCCGCTCC CCGAGGCTGG TGCGCGCTGG CTCCCGCATC
    CTGGACAAGC TGCAGTTCTT CGAGGAGCGC CGGCGCAGCC TGGAGCGCAG CGACTCGCCG
    CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG CGCAAGGCCC GTTCGCTGGA GCAGCCCAAG
    TCCGAGGGCG GCGGGCCGTG GGGCACGCCC AGGGCCTCGC AGGAGGAGCT GCGGTCCCCG
    GCGGGCAGCG TGGCCGAGCG GCGCCGCCTA TTCCAACAGA AGGCGGCCTC GCTGGACGAG
    CGCACGCGTC AGCGCAGCCC TGCGTCCGAC CTCGAGCTGC GCTTCGCCCA GGAGCTGGGC
    CGCATCCGCC GCTCCACGTC GCGGGAGGAG CTGGTGCGCT CTCACGAGTC CCTGCGCGCC
    ACGCTGCAGC GCGCCCCGTC CCCCCGAGAG CCCGGCGAGC CGCCGCTCTT CTCCCAGCCC
    TCCACCCCCA AGACCCCGCG GGCCTTGAGC CCCGCCGCCG CCCAGCCGCC CCCTGCGAGC
    GTCGCGGAAA AGCCCGGGGA TGAGCCAGGG AGGCCCCGGG GCCGTGGGCC AGCGGGCAGG
    ACGGAGCCGG GGGAAGGCCC GCAGCAGGAG GTTAGGCGCC GGGACCAGTT CCCGCTGACC
    CGGAGCAGAG CCATCCAGGA GTGCCGGAGC CCGGTGCCGC CCCCCACCTC TGAGCCCCCC
    GAGACCAGGA CGAAAGCTCC CCCGGGTCGG AAGCGGGAGC CCCCGCCGCA GGCCGTGCGC
    TTCCTGCCCT GGGCCACGCC GGGCCAGGAG GGCGCTGCTG TGCCCCAGAC CTTGGAGAAG
    AACAGGGCGG GACCCGAGGC CGAGAAGAGG CTGCGCAGAG GGCCAGAGGA GGACGGTCCT
    TGGGGGGCCT GGGACCGCCG AGGGGCCCGC AGCCAGGGCA GAGGTCGCCG GGCGCGGCCC
    ACCTCGCCTG AGCTCGAGTC TTCGGATGAC TCCTATGTGT CTGCTGGAGA AGAGCCTCTG
    GAGGCCCCTG TGTTTGAGAT CCCCCTGCAA AATGCAGTGG TGGCCCCAGG GGTGGACGTG
    CTGCTCAAGT GTATTGTTAC CGCCAACCCC CCGCCCCAAG TGTCCTGGCA GAAGGATGGG
    TCCCCGCTGC GCAGTGATGG CCGCCTTCTC ATCCGGGCAG AAGGCGAGCG GCACACCCTG
    CTGCTCCGGG AGGCCCGGGC CGCCGATGCC GGGAGCTATG CAGCCACTGC CACCAACGAG
    CTGGGCCAGG CCCGCTGCGC TGCCACGCTG GCCGTGAGAC CTGGTGGGTC AACATCTCCT
    TTCAGCAGTC CTATCACCTC TGACGAGGAG TATCTGAGCC CCCCAGAGGA GTTCCCGGAA
    CCCGGGGAGA CCTGGCCCCG AGCCCCCACC ATGAAGCTCA GTCCTGGCCA GAATCGCCGT
    TCCTCCGACA CTGGCTCCAA GGCGCCCCCT ACTTTCAAGG TTTCACTTAT GGACCAGTCA
    GTAAGGGAAG GCCAAGACGT CACCATGAGC ATCCGTGTGC AGGGCGAACC CAAGCCGGTG
    GTCTCCTGGC TGAGAAACCG TCAGCCTGTG CGCCCAGACC AGCGGCGCTT TGCTGAGGAG
    GCAGAGGATG GGCTCTGCCG GCTTCGGATC CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC
    TACACCTGCA AGGCGGTTAA CGAGTATGGC GCTCGACAGT GTGAGGCGCG CCTGGAGGTC
    AGAGCACACC CTGAAAGCCG GTCCCTGGCC GTGCTGGCCC CTCTGCAGGA CGTGGACGTG
    GGGGCCGGGG AGATGGCGCT GTTTGAGTGC CTGGTGGCGG GTCCCACCGA CGTGGAGGTA
    GACTGGCTGT GCCGAGGCCG CCTGCTGCAG CCTGCACTAC TCAAATGCAA GATGCATTTC
    GATGGCCGCA AATGCAAGCT GCTGCTCACC TCTGTGCATG AGGACGACAG CGGCGTCTAC
    ACCTGCAAGC TCAGCACGGC CAAAGATGAA CTGACCTGCA GTGCCCGGCT GACCGTGCGA
    CCCTCGCTGG CACCCCTGTT CACGCGGCTG CTGGAGGACG TGGAAGTGCT GGAGGGCCGC
    GCTGCCCGCT TCGACTGCAA GATCAGCGGG AGCCCGCCCC CCTCCGTTAC CTGGACTCAC
    TTTGGCCACC CCGTGGAGGA GAGCGAGAAC GTGCGGCTGC GGCAGGACGG GGGCCTGCAT
    TCGCTGCACA TCGCACATGT GGGCAGTGAG GATGAGGGGC TGTACGCGGT CAGTGCTGCC
    AACACCCACG GCCAGGCCCA CTGCTCAGCC CAGCTCTACG TGGAGGAGCC TCGGACGGCT
    GCCACCGGCC CTAGCTCCAA GCTGGAGAAG ATGCCCTCCA TCCCCGAGGA GCCCGAGCAG
    GGTGAGCTGG AGCGGCTGTC CATGCCCGAC TTCCTGCGGC CGCTGCAGGA TCTGGACGTG
    GGACTGGCCA AGGAGGCCAT GCTGGAGTGC CAGGTGACCG GCCTGCCCTA CCCCACCATC
    AGCTGGTTCC ACAACGGCCA CCGCATCCAG AGCAGCGACG ACCGACGCAT GACGCAGTAC
    AGGGATGTCC ATCGCTTGGT GTTCCCTGCC GTGGGACCTC AGCATGCTGG TGTCTACAAG
    AGCGTCATTG CTAACAAGCT GGGCAAAGCC GCCTGCTATG CTCATCTCTA CGTCACAGAT
    GTGGTTCCAG GCCCCCCAGA CGGTGCCCCT CAGGTAGTGG CTGTGACCGG GAGGATGATC
    ACGCTTGCAT GGAACCCCCC GAGGAGTCTG GACATGGCTA TCGACCCAGA CGCCCTGACC
    TACACCGTGC AGCACCAGGT GCTGGGCTCA GACCAGTGGA CGGCGCTGGT CACGGGCCTG
    CGGCAGCCCG GGTGGGCAGC CACCGGATTG CGAAAGGGGG TCCAGCACGT CTTCCGGGTT
    CTCAGCACCA CCATCAAGAG CAGCAGCAAG CCCTCGCCCC CGTCCGAGCC CACGCAGCTG
    CTGGAGCGTG GCCCGCCCCT GGAGGAGGCG CCCGCTGTGC TGGACAAGCC AGACGTCGTG
    TATGTGGTGG AGGGCCAGCC CACCAGTGTC ACCGTCACCT TCAACCACGT GGAGGCCCAG
    GTTGTCTGGA GGAGCTGCCG AGGAGCCCTC CTAGAGGCCC GGCCAGGTGT GTTTGAGCTG
    AGCCAGCCAG ATGACGACCA GTACTCTCTT AGGATCTGCC GGGTGAGCCG CCGGGACCTG
    GGAGCCCTCA CTTGCACTGC CCGCAACCGC CACGGCACAC AGGCCTGCTC AGTCACCCTG
    GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC ATCATGGAGG ATGTGGAGGT AGGGGCTGGA
    GAGACTGCTC GCTTCGCCGT GGTGGTGGAC GGGAAGCCCT TGCCGGACAT CATGTGGTAC
    AAGGATGAGG CGCTGCTGAC CGAGAGCAAC CATGTGAGCT TCGTGTACGA GGAGAACGAG
    TGCTCCCTGG TGGTGCTCAG CGCGGGGGCC CAGGATGGAG GGGTCTACAC CTGCACGGCC
    CGTAACCTGG CTGGCGAAGT CTCCTGCAAA GCAGAGCTGG CTGTGCACTC AGCTCAGACA
    GCTATGGAGG TGGAAGGGAT CGGGGAAGAC GAGGAGCAGC GAGGAAGGAG GCTCAGCGAC
    TTCTATGATA TTCAGCAGGA GATCGGCAGG GGTGCCTTCT CCTACCTGCG CCGTGTAGTG
    GAGAGGAGCT CCGGACTGGA GTTTGCAGCC AAGTTCATCC CCAGCCAGGC AAAGCCAAAG
    GCATCGGCAT GGCGGGAGGC ACGGCTGCTG GCCCGGCTCC AGCATGACTG CATCCTCTAC
    TTCCATGAGG CCTTCGAGAG GCGCCGGGGA CTGGTCATCG TCACCGAGCT CTGCACAGAA
    GAGCTTCTGG AGCGAATGGC CAGGAAACCC ACCGTGTGTG AGTCTGAGAT CCGGGCCTAC
    ATGCGGCAGG TACTAGAAGG AATATGCTAC TTGCACGAGA ACCACGTGCT GCACCTGGAT
    GTCAAGCCCG AGAACCTGCT GGTGTGGGAT GGTGTTGAGG GCGAGGAGCA GGTGAGGATC
    TGTGACTTTG GGAACGCTCA AGAGCTGACA CCGGGAGAGC CCCAGTATTG CCAATACGGC
    ACACCTGAGT TCGTGGCGCC CGAGATTGTC AACCAGACCC CTGTGTCTGG AGTCACTGAC
    ATCTGGCCCG TGGGTGTCGT CGCCTTCCTC TGCCTGACCG GAATCTCCCC GTTCGTTGGG
    GAAAATGATC GGACGACATT GATGAACATC CGAAACTACA ACGTGGCCTT CGAGGAGACC
    ACGTTCCTGA GCCTGAGCAG GGAGGCCCGG GGCTTCCTCA TCAAAGTGCT GGTGCGGGAC
    CGGCTGAGGC CTACTGCAGA GGAGACCCTG GAACATCCTT GGTTTAAAGC ACAGGCAAAG
    GGTGCAGTGG TGAGCACGGA TCACCTAAAG CTATTCCTCT CCCGGCGGAG GTGGCAGCGC
    TCCCAGATCA GCTACAAGTG CCACCTGGTG CTGCGCCCCA TCCCGGAGCT GCTGCGAGCA
    CCCCCGGAGC GGGTGTGGGT GGCCGTGCCC CGAAGACCAC CCCCTAGTGG AGGGCTGTCG
    TCATCATCCG ACTCTGAAGA GGAAGAGCTG GAGGAGCTGC CCTCTGTGCC CCGACCTCTG
    CAGCCTGAGT TTTCGGGCTC CCGGGTGTCC CTCACTGACA TTCCCACTGA GGATGAGGCC
    CTCGGGGCCC TGGAGGCTGG GGCGGCCACC CCCATGGACT GGCAAGAACA AGGAAAGGCC
    CCCTCTCAGG ACCAGGAGGC CCCCAGCCCT CAGGCACTCC CCTCCCCAGG CCAGGAGTCC
    CCGTCCGGGC CCAGCCCCCG GCGGGCAGAG CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC
    CTGCCCCGGG CCGGGCCGAG GGAACCGGGC CGGGGCCTGC ACAAGGCAGC GTCCGTCGAG
    CTGCCGCAGC GCCGAAGCCC CAGCCCGGGG GCCACCCGCC TGACCCGAGG AGGCCTGGGT
    GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC CTGCGCCAGC GGCTGCTGAG GGGTGGCCCC
    GATGATGGCA AGGTCAGCGG CCTCAGGGGC CCCCTGCTGG AGAGCCTGGG GGGCCGGGCT
    CGTGACCCCC GGCTGGCGCG GGCAGCCTCC AGCGAGGCAG CACCCCATCA CCAGCACCCA
    CCTGAGGCTC GGGGCCTGCA GAAGAGCAGT AGCTTCTCGC AGGGTGAGGC AGAGCCCCGG
    GGCCGGCACC GCCGAGCAGG GGCACCCCTT GAGATCCCCG TGGCCCGGCT GGGGGCCCGC
    AAGCTGCAGG AGTCTCCCTC CTTGTCCGCC CTCAGCGAGG CCCAACCACC TAGCCCCGTG
    CGGCCCAGTG CAGCCAAGCC CAGCACCCCT AAGTCTTCAG AACCTCTTGT GGGCACCCCC
    AGTGAGGCTG CACAGCCTCC AGCACCCCAG CCTGCCCGAG AGAAGGCCCC AGAATCTACG
    CCAGAACCAG CTCGGGGCCC CAAGCCCACA CAGCCCCCCA CAGCTCTGCA AACCCTAGCG
    CCTCCTCTCA CTCCGTATGC GCAGATCATG CAGTCGCTCC AGCTGCCAGG CCAGGCGCAA
    GGCCCCCCAC AGGGCGCAGT CGTATCTCCT ACATCTCGGT CTCCAGCATC TCGGTTGGAA
    CCCAAGCCCC ACCCGGCTGT GTTCTCCAGG GTGGCCTCCC CACCTCCAGG AGCCTCCGAG
    AAGCGCCTGC TGCCGGGAGC CGGAGCAACC CCAGGGCCAG CCGAAAAAGC CCGGGTCCCG
    GCGGTCCCCC GCAGGCCAGG GAGCAGTCTC AGCAGCAGCA TCGAGAACCT GGAGTCGGAG
    GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC CGGGAGTCGC CCCTGTCGCG GGGGCTGCGG
    CTGCTGAGCC GCTCGCGGTC GGAGGAGCGC GGCCCCTTCC GCGGGGCTGA GGAGGAGGAC
    GGCATTTACC GGCCCAGCCC GGCGGGCACG CCGCTGGAGC TGGTGCGACG GCCCGAGCGC
    TCGCGCTCCG TGCAGGACCT CAGGGCGGTC GGGGAGCCAG GCCTCGTGCG CCGCCTCTCG
    CTGTCGCTGT CCCAGCGTCT GCGGCGGACC CCGCCCGCGC AGCGCCACCC GGCCTGGGAG
    CCCCGCGGCG GGGACGGCGA GAGCTCGGAG GGCGGCAGCT CGGCGCGGGG CTCCCCGGTG
    CTGACGGTGC GCCGGAGGCT CAGCTCCACG CTGGAGCGGC TGTCGAGCCG GTTGCAGCGC
    AGCGGCAGCA GCGAGGACTC GGGGGGCGCG TCGGGCCGCA GCACGCCGCT GTTCGGCCGG
    CTGCGCAGGG CCACGTCCGA GAGCGAGAGT CTGCGGCGCC TGGACCTCGC GCAAAATCAG
    CTGGCGGCCC AAGCCGGCGC CGCCACCCCT TCCGCGGAGT CCCTGGGCTC CGAGGCCAGC
    GCTACGTCGG GCTCCTCTGC TCCTGCAGAA AGTCGAAGCC GCCTGCGCTG GGGCCTCTCT
    CGGCTGCGGA AGGACAAGAG GTTGTCACAG CCTAACCTCT CTGGCAGTGT CCAGGAGGAT
    TTGGGTCACC AGTATGTGCG CAGTGAGTCA GACTTTCCCC CAGTCTTCCA CATCAAACTC
    AAGGACCAGG TGCTGCTGGA GGGAGAGGCA GCCACCCTGC TCTGCTTGCC GGCAGCCTGC
    CCTGCACCCC ACATCTCCTG GATGAAAGAC AAGCAGTCCC TGCGGTCAGA GCCCTCGGTG
    ATCATCGTGT CCTGCAAAGA TGGTCGTCAG CTACTGAGCA TCCCCCGGGC AGGCAAGCGG
    CATGCGGGGC TCTATGAGTG CTCCGCCACC AACGTCCTTG GCAGCATCAC CAGCTCCTGT
    ACCGTGGCTG TGGCTCGTAC CCCAGGAAAG CTAGCTCCTC CTGAGGTGCC CCAGACCTAC
    CAGGACACAG CACTGGTGCT CTGGAAGCCG GGAGACAGCA AGGCGCCTTG CACATACACG
    CTGGAGCGGC GGGTGGATGG GGAGTCTTCC TGGCACCCTG TGAGCTCGGG CATCCCCGAC
    TGCTACTACA ACGTGACCCA CCTACCAGTC GGCATGGCTG TGAGGTTCCG TGTGGCCTGT
    GCCAACCGTG CTGGGCAAGG GCCTTTCAGC AACCCTTCTG AGAAGGTCCT CGTCAGGGGC
    GTGCAAGATT CCTCAGCTCT GCCATCTGCT GCTCACAGAG ATGCCCCTGT TACCTCAGGG
    CCAGCCAGGG CCCCGCCTCC TGACGCTCCT ACCTCACTGG CCCCTCCCCT GAGCCCCTCC
    CCAGCTCCTG CTCCCCCAGG CCCCCAGGCA GCCCCTCTCG GCCCTTCATC TTCCCCAGCA
    CCCCCCAGCC AGGCCTTGTC CTCCCTCAAG GCTGTGGGTC CACCACCCCA AACCCCCCCA
    CGAAAGCACC GGGGCCTGCA GGCTGCCCAG CAAGTAGAAC CTACCCCACC CAGTGCCCAG
    GACTCCCCAA GTGAGCCCAA GTCTTCCGTC CCTGACACTG GGACCCCCAT CCCAGCCTCT
    GCCCCTCAAG TGGTGAAAAC AGCCTCTTCC TCAATGCCCC TGTATATGGT GACCTCCTTT
    GTGTCTGCAC CACCAGCCCC TGAGCCCCCA GCCCCCGAGC CCCCTCCTGA GCCCACCAAA
    GTGACTGTGC GGAGCCTCAG CCCAGCCAAG GAGGTGGTTG GCTCCCCTGG GGGTGGTCCC
    CCCAGGGCTC CTAAGCCAGA GGGCACCACT CTTCGACAGG GCCCTCCTCA GAAACCATAC
    ACCTTCCTGG AGGAGAAGGC CAGGGGTCGC TTTGGTGTGG TGCGTGCCTG CCGGGAGAAT
    GCCACCGGGC GAACATTCGT GGCCAAGATT GTGCCATACG CGGCCGAGGG CAAGCGGCAG
    GTCCTGCAGG AGTACGAGGT GCTGAGGACC CTGCACCACG AGCGGCTTAT GGCCCTGCAC
    GAAGCCTACA TCACCCCGCG CTACCTCGTG CTCATCGCCG AGAGCTGCGG CAACCGGGAG
    CTTCTCTGTG GGCTCAGCGA CAGATTCCGG TATTCAGAGG ATGATGTGGC CACCTATGTG
    GTGCAGCTGC TACAAGGCCT GGATTACCTC CATGGCCGCC ACGTGCTGCA CCTGGACATC
    AAGCCAGACA ACCTGCTGCT GGCCCCTGAC AACGCCCTCA AGATCGTGGA CTTTGGCAGT
    GCCCAGCCCT ACAACCCCCA GGCTCTGCGG CCCCTGGGTC ACCGCACGGG CACACTGGAG
    TTCATGGCTC CTGAGATGGT AAAGGGAGAC CCCATCGGCT CTGCCACGGA CATCTGGGGA
    GCGGGTGTGC TCACTTACAT CATGCTCAGT GGACGCTCCC CATTCTATGA GCCAGACCCC
    CAGGAAACAG AGGCTCGCAT TGTGGGGGGC CGCTTTGATG CCTTCCAGCT GTATCCCAAC
    ACATCCCAGA GTGCCACCCT CTTCTTGCGA AAGGTCCTCT CAGTACATCC CTGGAGCCGG
    CCCTCCGTGC AGGACTGCCT GGCTCACCCG TGGCTGCAGG ATGCCTACCT GATGAAACTG
    CGCCGCCAGA CACTCACCTT CACCACCAAC CGGCTCAAGG AGTTCCTGGG TGAGCAGCGG
    CGCCGCAGGG CCGAGGCCGC CACCCGCCAC AAGGTGCTGC TCCGCTCTTA CCCAGGCAGC
    CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa245707
    Clone ID Related Accession (Same CDS sequence) NM_001142485.3
    Accession Version NM_001142485.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-15
    Organism Bos taurus(cattle)
    Product striated muscle preferentially expressed protein kinase
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000002.1 and EV798029.1. On Apr 16, 2019 this sequence version replaced NM_001142485.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145420 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCGGGCA CGAGGGCACC CCCCAGCCCC 
    GGGGTGCCCC CGAAGAGGGC CAAAGTGGGG GCCGGCGGAG GGGCGGCGGC CGCGGTGGCC
    GGGGCGCCGG TCTTCCTACG GCCCCTGAAG GACGCGGCGG TGTCTGCGGG CAGCGACGTG
    CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCAGCTGGTT CCGGGATGGG
    CAGCGCTTGC CCCCGCCGGC CCCTGAGCCC AGCTGCCTGT GGCTGCGAAG CTGCGGGCCG
    CGGGATGCCG GCGTGTACAG CTGCAGGGCC CAGAACGAGC TGGGCCAGGC CTCCTGCGAG
    GCGGTTCTCA CCGTGCTGGA GGTCGGAGAC TCGGAGACAG GCGAGGATGA CATCAGCGAC
    GGGCAGGGGA CCCAGCGCCT GGAGCTTCGG GATGATGGGG CCTTCAGCAC CCCCACGGGG
    GGCTCTGATA CCCTGGTGGG CACCTCCCTG GACACGCCCC CGACCTCCGT GACAGGCACC
    TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC
    AGCAGGGCTG GCACCCGCCG CCTCCCGGGC AGCCCAAGGC AAGCACAGGC AACCGGGGCC
    GGGCCACGGC ACCTGGGGGT GGAGCCGCTC GTACGGGCAT CTCGAGCTAA TCTGGTGGGC
    GCAAGCTGGG GGTCAGAGGA TAGCCTTTCG GTGGCCAGTG ACCTGTACGG CAGCGCATTC
    AGCCTGTACA GAGGACGGGC GCTCTCGATC CACGTCAGTG TCCCTCAGAG CGGACTGCAC
    AAGGAGGAGC CTGACCTTCA GCCTCAGCCC GCCAGCGAAG CCCCCCGTCG CCCAGCCCTG
    CCGCCCCCCT CCAAATCCGC GCTGCTGCCC CCACCGTCCC CTCGGGTGGG TAAGCGGCCC
    CCGCCGGGAG CCGGCGGCCA GCCCCTGGCC ACCCCCACGC CGCCGCACCG GCGCTCTCGG
    GAGCCGGTGC CGCCCGAGGA CGCCACCGCC GAAGAAAAGC GAGGGAAAAA GTCCAAGTCG
    TCCGGGCCCT CGCTAGCGGG CACGGCGGAG TCCCGGCCCC AGACGCCCCT GAGTGAGGCC
    TCGGGCCGCC AGTCGGCGCT GGGCCGCTCC CCGAGGCTGG TGCGCGCTGG CTCCCGCATC
    CTGGACAAGC TGCAGTTCTT CGAGGAGCGC CGGCGCAGCC TGGAGCGCAG CGACTCGCCG
    CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG CGCAAGGCCC GTTCGCTGGA GCAGCCCAAG
    TCCGAGGGCG GCGGGCCGTG GGGCACGCCC AGGGCCTCGC AGGAGGAGCT GCGGTCCCCG
    GCGGGCAGCG TGGCCGAGCG GCGCCGCCTA TTCCAACAGA AGGCGGCCTC GCTGGACGAG
    CGCACGCGTC AGCGCAGCCC TGCGTCCGAC CTCGAGCTGC GCTTCGCCCA GGAGCTGGGC
    CGCATCCGCC GCTCCACGTC GCGGGAGGAG CTGGTGCGCT CTCACGAGTC CCTGCGCGCC
    ACGCTGCAGC GCGCCCCGTC CCCACGAGAG CCCGGCGAGC CGCCGCTCTT CTCCCAGCCC
    TCCACCCCCA AGACCCCGCG GGCCTTGAGC CCCGCCGCCG CCCAGCCGCC CCCTGCGAGC
    GTCGCGGAAA AGCCCGGGGA TGAGCCAGGG AGGCCCCGGG GCCGTGGGCC AGCGGGCAGG
    ACGGAGCCGG GGGAAGGCCC GCAGCAGGAG GTTAGGCGCC GGGACCAGTT CCCGCTGACC
    CGGAGCAGAG CCATCCAGGA GTGCCGGAGC CCGGTGCCGC CCCCCACCTC TGAGCCCCCC
    GAGACCAGGA CGAAAGCTCC CCCGGGTCGG AAGCGGGAGC CCCCGCCGCA GGCCGTGCGC
    TTCCTGCCCT GGGCCACGCC GGGCCAGGAG GGCGCTGCTG TGCCCCAGAC CTTGGAGAAG
    AACAGGGCGG GACCCGAGGC CGAGAAGAGG CTGCGCAGAG GGCCAGAGGA GGACGGTCCT
    TGGGGGGCCT GGGACCGCCG AGGGGCCCGC AGCCAGGGCA GAGGTCGCCG GGCGCGGCCC
    ACCTCGCCTG AGCTCGAGTC TTCGGATGAC TCCTATGTGT CTGCTGGAGA AGAGCCTCTG
    GAGGCCCCTG TGTTTGAGAT CCCCCTGCAA AATGCAGTGG TGGCCCCAGG GGTGGACGTG
    CTGCTCAAGT GTATTGTTAC CGCCAACCCC CCGCCCCAAG TGTCCTGGCA GAAGGATGGG
    TCCCCGCTGC GCAGTGATGG CCGCCTTCTC ATCCGGGCAG AAGGCGAGCG GCACACCCTG
    CTGCTCCGGG AGGCCCGGGC CGCCGATGCC GGGAGCTATG CAGCCACTGC CACCAACGAG
    CTGGGCCAGG CCCGCTGCGC TGCCACGCTG GCCGTGAGAC CTGGTGGGTC AACATCTCCT
    TTCAGCAGTC CTATCACCTC TGACGAGGAG TATCTGAGCC CCCCAGAGGA GTTCCCGGAA
    CCCGGGGAGA CCTGGCCCCG AGCCCCCACC ATGAAGCTCA GTCCTGGCCA GAATCGCCGT
    TCCTCCGACA CTGGCTCCAA GGCGCCCCCT ACTTTCAAGG TTTCACTTAT GGACCAGTCA
    GTAAGGGAAG GCCAAGACGT CACCATGAGC ATCCGTGTGC AGGGCGAACC CAAGCCGGTG
    GTCTCCTGGC TGAGAAACCG TCAGCCTGTG CGCCCAGACC AGCGGCGCTT TGCTGAGGAG
    GCAGAGGATG GGCTCTGCCG GCTTCGGATC CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC
    TACACCTGCA AGGCGGTTAA CGAGTATGGC GCTCGACAGT GTGAGGCGCG CCTGGAGGTC
    AGAGCACACC CTGAAAGCCG GTCCCTGGCC GTGCTGGCCC CTCTGCAGGA CGTGGACGTG
    GGGGCCGGGG AGATGGCGCT GTTTGAGTGC CTGGTGGCGG GTCCCACCGA CGTGGAGGTA
    GACTGGCTGT GCCGAGGCCG CCTGCTGCAG CCTGCACTAC TCAAATGCAA GATGCATTTC
    GATGGCCGCA AATGCAAGCT GCTGCTCACC TCTGTGCATG AGGACGACAG CGGCGTCTAC
    ACCTGCAAGC TCAGCACGGC CAAAGATGAA CTGACCTGCA GTGCCCGGCT GACCGTGCGA
    CCCTCGCTGG CACCCCTGTT CACGCGGCTG CTGGAGGACG TGGAAGTGCT GGAGGGCCGC
    GCTGCCCGCT TCGACTGCAA GATCAGCGGG AGCCCGCCCC CCTCCGTTAC CTGGACTCAC
    TTTGGCCACC CCGTGGAGGA GAGCGAGAAC GTGCGGCTGC GGCAGGACGG GGGCCTGCAT
    TCGCTGCACA TCGCACATGT GGGCAGTGAG GATGAGGGGC TGTACGCGGT CAGTGCTGCC
    AACACCCACG GCCAGGCCCA CTGCTCAGCC CAGCTCTACG TGGAGGAGCC TCGGACGGCT
    GCCACCGGCC CTAGCTCCAA GCTGGAGAAG ATGCCCTCCA TCCCCGAGGA GCCCGAGCAG
    GGTGAGCTGG AGCGGCTGTC CATGCCCGAC TTCCTGCGGC CGCTGCAGGA TCTGGACGTG
    GGACTGGCCA AGGAGGCCAT GCTGGAGTGC CAGGTGACCG GCCTGCCCTA CCCCACCATC
    AGCTGGTTCC ACAACGGCCA CCGCATCCAG AGCAGCGACG ACCGACGCAT GACGCAGTAC
    AGGGATGTCC ATCGCTTGGT GTTCCCTGCC GTGGGACCTC AGCATGCTGG TGTCTACAAG
    AGCGTCATTG CTAACAAGCT GGGCAAAGCC GCCTGCTATG CTCATCTCTA CGTCACAGAT
    GTGGTTCCAG GCCCCCCAGA CGGTGCCCCT CAGGTAGTGG CTGTGACCGG GAGGATGATC
    ACGCTTGCAT GGAACCCCCC GAGGAGTCTG GACATGGCTA TCGACCCAGA CGCCCTGACC
    TACACCGTGC AGCACCAGGT GCTGGGCTCA GACCAGTGGA CGGCGCTGGT CACGGGCCTG
    CGGCAGCCCG GGTGGGCAGC CACCGGATTG CGAAAGGGGG TCCAGCACGT CTTCCGGGTT
    CTCAGCACCA CCATCAAGAG CAGCAGCAAG CCCTCGCCCC CGTCCGAGCC CACGCAGCTG
    CTGGAGCGTG GCCCGCCCCT GGAGGAGGCG CCCGCTGTGC TGGACAAGCC AGACGTCGTG
    TATGTGGTGG AGGGCCAGCC CACCAGTGTC ACCGTCACCT TCAACCACGT GGAGGCCCAG
    GTTGTCTGGA GGAGCTGCCG AGGAGCCCTC CTAGAGGCCC GGCCAGGTGT GTTTGAGCTG
    AGCCAGCCAG ATGACGACCA GTACTCTCTT AGGATCTGCC GGGTGAGCCG CCGGGACCTG
    GGAGCCCTCA CTTGCACTGC CCGCAACCGC CACGGCACAC AGGCCTGCTC AGTCACCCTG
    GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC ATCATGGAGG ATGTGGAGGT AGGGGCTGGA
    GAGACTGCTC GCTTCGCCGT GGTGGTGGAC GGGAAGCCCT TGCCGGACAT CATGTGGTAC
    AAGGATGAGG CGCTGCTGAC CGAGAGCAAC CATGTGAGCT TCGTGTACGA GGAGAACGAG
    TGCTCCCTGG TGGTGCTCAG CGCGGGGGCC CAGGATGGAG GGGTCTACAC CTGCACGGCC
    CGTAACCTGG CTGGCGAAGT CTCCTGCAAA GCAGAGCTGG CTGTGCACTC AGCTCAGACA
    GCTATGGAGG TGGAAGGGAT CGGGGAAGAC GAGGAGCAGC GAGGAAGGAG GCTCAGCGAC
    TTCTATGATA TTCAGCAGGA GATCGGCAGG GGTGCCTTCT CCTACCTGCG CCGTGTAGTG
    GAGAGGAGCT CCGGACTGGA GTTTGCAGCC AAGTTCATCC CCAGCCAGGC AAAGCCAAAG
    GCATCGGCGT GGCGGGAGGC ACGGCTGCTG GCCCGGCTCC AGCATGACTG CATCCTCTAC
    TTCCATGAGG CCTTCGAGAG GCGCCGGGGA CTGGTCATCG TCACCGAGCT CTGCACAGAA
    GAGCTTCTGG AGCGAATGGC CAGGAAACCC ACCGTGTGTG AGTCTGAGAT CCGGGCCTAC
    ATGCGGCAGG TACTAGAAGG AATATGCTAC TTGCACGAGA ACCACGTGCT GCACCTGGAT
    GTCAAGCCCG AGAACCTGCT GGTGTGGGAT GGTGTTGAGG GCGAGGAGCA GGTGAGGATC
    TGTGACTTTG GGAACGCTCA AGAGCTGACA CCGGGAGAGC CCCAGTATTG CCAATACGGC
    ACACCTGAGT TCGTGGCGCC CGAGATTGTC AACCAGACCC CTGTGTCTGG AGTCACTGAC
    ATCTGGCCCG TGGGTGTCGT CGCCTTCCTC TGCCTGACCG GAATCTCCCC GTTCGTTGGG
    GAAAATGATC GGACGACATT GATGAACATC CGAAACTACA ACGTGGCCTT CGAGGAGACC
    ACGTTCCTGA GCCTGAGCAG GGAGGCCCGG GGCTTCCTCA TCAAAGTGCT GGTGCGGGAC
    CGGCTGAGGC CTACTGCAGA GGAGACCCTG GAACATCCTT GGTTTAAAGC ACAGGCAAAG
    GGTGCAGTGG TGAGCACGGA TCACCTAAAG CTATTCCTCT CCCGGCGGAG GTGGCAGCGC
    TCCCAGATCA GCTACAAGTG CCACCTGGTG CTGCGCCCCA TCCCGGAGCT GCTGCGAGCA
    CCCCCGGAGC GGGTGTGGGT GGCCGTGCCC CGAAGACCAC CCCCTAGTGG AGGGCTGTCG
    TCATCATCCG ACTCTGAAGA GGAAGAGCTG GAGGAGCTGC CCTCTGTGCC CCGACCTCTG
    CAGCCTGAGT TTTCGGGCTC CCGGGTGTCC CTCACTGACA TTCCCACTGA GGATGAGGCC
    CTCGGGGCCC TGGAGGCTGG GGCGGCCACC CCCATGGACT GGCAAGAACA AGGAAAGGCC
    CCCTCTCAGG ACCAGGAGGC CCCCAGCCCT CAGGCACTCC CCTCCCCAGG CCAGGAGTCC
    CCGTCCGGGC CCAGCCCCCG GCGGGCAGAG CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC
    CTGCCCCGGG CCGGGCCGAG GGAACCGGGC CGGGGCCTGC ACAAGGCAGC GTCCGTCGAG
    CTGCCGCAGC GCCGAAGCCC CAGCCCGGGG GCCACCCGCC TGACCCGAGG AGGCCTGGGT
    GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC CTGCGCCAGC GGCTGCTGAG GGGTGGCCCC
    GATGATGGCA AGGTCAGCGG CCTCAGGGGC CCCCTGCTGG AGAGCCTGGG GGGCCGGGCT
    CGTGACCCCC GGCTGGCGCG GGCAGCCTCC AGCGAGGCAG CACCCCATCA CCAGCACCCA
    CCTGAGGCTC GGGGCCTGCA GAAGAGCAGT AGCTTCTCGC AGGGTGAGGC AGAGCCCCGG
    GGCCGGCACC GCCGAGCAGG GGCACCCCTT GAGATCCCCG TGGCCCGGCT GGGGGCCCGC
    AAGCTGCAGG AGTCTCCCTC CTTGTCCGCC CTCAGCGAGG CCCAACCACC TAGCCCCGTG
    CGGCCCAGTG CAGCCAAGCC CAGCACCCCT AAGTCTTCAG AACCTCTTGT GGGCACCCCC
    AGTGAGGCTG CACAGCCTCC AGCACCCCAG CCTGCCCGAG AGAAGGCCCC AGAATCTACG
    CCAGAACCAG CTCGGGGCCC CAAGCCCACA CAGCCCCCCA CAGCTCTGCA AACCCTAGCG
    CCTCCTCTCA CTCCGTATGC GCAGATCATG CAGTCGCTCC AGCTGCCAGG CCAGGCGCAA
    GGCCCCCCAC AGGGCGCAGT CGTATCTCCT ACATCTCGGT CTCCAGCATC TCGGTTGGAA
    CCCAAGCCCC ACCCGGCTGT GTTCTCCAGG GTGGCCTCCC CACCTCCAGG AGCCTCCGAG
    AAGCGCCTGC TGCCGGGAGC CGGAGCAACC CCAGGGCCAG CCGAAAAAGC CCGGGTCCCG
    GCGGTCCCCC GCAGGCCAGG GAGCAGTCTC AGCAGCAGCA TCGAGAACCT GGAGTCGGAG
    GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC CGGGAGTCGC CCCTGTCGCG GGGGCTGCGG
    CTGCTGAGCC GCTCGCGGTC GGAGGAGCGC GGCCCCTTCC GCGGGGCTGA GGAGGAGGAC
    GGCATTTACC GGCCCAGCCC GGCGGGCACG CCGCTGGAGC TGGTGCGACG GCCCGAGCGC
    TCGCGCTCCG TGCAGGACCT CAGGGCGGTC GGGGAGCCAG GCCTCGTGCG CCGCCTCTCG
    CTGTCGCTGT CCCAGCGTCT GCGGCGGACC CCGCCCGCGC AGCGCCACCC GGCCTGGGAG
    CCCCGCGGCG GGGACGGCGA GAGCTCGGAG GGCGGCAGCT CGGCGCGGGG CTCCCCGGTG
    CTGACGGTGC GCCGGAGGCT CAGCTCCACG CTGGAGCGGC TGTCGAGCCG GTTGCAGCGC
    AGCGGCAGCA GCGAGGACTC GGGGGGCGCG TCGGGCCGCA GCACGCCGCT GTTCGGCCGG
    CTGCGCAGGG CCACGTCCGA GAGCGAGAGT CTGCGGCGCC TGGACCTCGC GCAAAATCAG
    CTGGCGGCCC AAGCCGGCGC CGCCACCCCT TCCGCGGAGT CCCTGGGCTC CGAGGCCAGC
    GCTACGTCGG GCTCCTCTGC TCCTGCAGAA AGTCGAAGCC GCCTGCGCTG GGGCCTCTCT
    CGGCTGCGGA AGGACAAGAG GTTGTCACAG CCTAACCTCT CTGGCAGTGT CCAGGAGGAT
    TTGGGTCACC AGTATGTGCG CAGTGAGTCA GACTTTCCCC CAGTCTTCCA CATCAAACTC
    AAGGACCAGG TGCTGCTGGA GGGAGAGGCA GCCACCCTGC TCTGCTTGCC AGCAGCCTGC
    CCTGCACCCC ACATCTCCTG GATGAAAGAC AAGCAGTCCC TGCGGTCAGA GCCCTCGGTG
    ATCATCGTGT CCTGCAAAGA TGGTCGTCAG CTACTGAGCA TCCCCCGGGC GGGCAAGCGG
    CATGCGGGGC TCTATGAGTG CTCCGCCACC AACGTCCTTG GCAGCATCAC CAGCTCCTGT
    ACCGTGGCTG TGGCTCGTAC CCCAGGAAAG CTAGCTCCTC CTGAGGTGCC CCAGACCTAC
    CAGGACACAG CACTGGTGCT CTGGAAGCCG GGAGACAGCA AGGCGCCTTG CACATACACG
    CTGGAGCGGC GGGTGGATGG GGAGTCTTCC TGGCACCCCG TGAGCTCGGG CATCCCCGAC
    TGCTACTACA ACGTGACCCA CCTACCAGTC GGCATGGCTG TGAGGTTCCG TGTGGCCTGT
    GCCAACCGTG CTGGGCAAGG GCCTTTCAGC AACCCTTCTG AGAAGGTCCT CGTCAGGGGC
    GTGCAAGATT CCTCAGCTCT GCCATCTGCT GCTCACAGAG ATGCCCCTGT TACCTCAGGG
    CCAGCCAGGG CCCCGCCTCC TGACGCTCCT ACCTCACTGG CCCCTCCCCT GAGCCCCTCC
    CCAGCTCCTG CTCCCCCAGG CCCCCAGGCA GCCCCTCTCG GCCCTTCATC TTCCCCAGCA
    CCCCCCAGCC AGGCCTTGTC CTCCCTCAAG GCTGTGGGTC CACCACCCCA AACCCCCCCA
    CGAAAGCACC GGGGCCTGCA GGCTGCCCAG CAAGTAGAAC CTACCCCACC CAGTGCCCAG
    GACTCCCCAA GTGAGCCCAA GTCTTCCGTC CCTGACACTG GGACCCCCAT CCCAGCCTCT
    GCCCCTCAAG TGGTGAAAAC AGCCTCTTCC TCAATGCCCC TGTATATGGT GACCTCCTTT
    GTGTCTGCAC CACCAGCCCC TGAGCCCCCA GCCCCCGAGC CCCCTCCTGA GCCCACCAAA
    GTGACTGTGC GGAGCCTCAG CCCAGCCAAG GAGGTGGTTG GCTCCCCTGG GGGTGGTCCC
    CCCAGGGCTC CTAAGCCAGA GGGCACCACT CTTCGACAGG GCCCTCCTCA GAAACCATAC
    ACCTTCCTGG AGGAGAAGGC CAGGGGTCGC TTTGGTGTGG TGCGTGCCTG CCGGGAGAAT
    GCCACCGGGC GAACATTCGT GGCCAAGATT GTGCCATACG CGGCCGAGGG CAAGCGGCAG
    GTCCTGCAGG AGTACGAGGT GCTGAGGACC CTGCACCACG AGCGGCTTAT GGCCCTGCAC
    GAAGCCTACA TCACCCCGCG CTACCTCGTG CTCATCGCCG AGAGCTGCGG CAACCGGGAG
    CTTCTCTGTG GGCTCAGCGA CAGATTCCGG TATTCAGAGG ATGATGTGGC CACCTATGTG
    GTGCAGCTGC TACAAGGCCT GGATTACCTC CATGGCCGCC ACGTGCTGCA CCTGGACATC
    AAGCCAGACA ACCTGCTGCT GGCCCCTGAC AACGCCCTCA AGATCGTGGA CTTTGGCAGT
    GCCCAGCCCT ACAACCCCCA GGCTCTGCGG CCCCTGGGTC ACCGCACGGG CACACTGGAG
    TTCATGGCTC CTGAGATGGT AAAGGGAGAC CCCATCGGCT CTGCCACGGA CATCTGGGGA
    GCGGGTGTGC TCACTTACAT CATGCTCAGT GGACGCTCCC CATTCTATGA GCCAGACCCC
    CAGGAAACAG AGGCTCGCAT TGTGGGGGGC CGCTTTGATG CCTTCCAGCT GTATCCCAAC
    ACATCCCAGA GTGCCACCCT CTTCTTGCGA AAGGTCCTCT CAGTACATCC CTGGAGCCGG
    CCCTCCGTGC AGGACTGCCT GGCTCACCCG TGGCTGCAGG ATGCCTACCT GATGAAACTG
    CGCCGCCAGA CACTCACCTT CACCACCAAC CGGCTCAAGG AGTTCCTGGG TGAGCAGCGG
    CGCCGCAGGG CCGAGGCCGC CACCCGCCAC AAGGTGCTGC TCCGCTCTTA CCCAGGCAGC
    CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001135957.2
    CDS1..9846
    Translation

    Target ORF information:

    RefSeq Version NM_001142485.3
    Organism Bos taurus(cattle)
    Definition Bos taurus striated muscle enriched protein kinase (SPEG), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001142485.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCGGGCA CGAGGGCACC CCCCAGCCCC 
    GGGGTGCCCC CGAAGAGGGC CAAAGTGGGG GCCGGCGGAG GGGCGGCGGC CGCGGTGGCC
    GGGGCGCCGG TCTTCCTACG GCCCCTGAAG GACGCGGCGG TGTCTGCGGG CAGCGACGTG
    CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCAGCTGGTT CCGGGATGGG
    CAGCGCTTGC CCCCGCCGGC CCCTGAGCCC AGCTGCCTGT GGCTGCGAAG CTGCGGGCCG
    CGGGATGCCG GCGTGTACAG CTGCAGGGCC CAGAACGAGC TGGGCCAGGC CTCCTGCGAG
    GCGGTTCTCA CCGTGCTGGA GGTCGGAGAC TCGGAGACAG GCGAGGATGA CATCAGCGAC
    GGGCAGGGGA CCCAGCGCCT GGAGCTTCGG GATGATGGGG CCTTCAGCAC CCCCACGGGG
    GGCTCTGATA CCCTGGTGGG CACCTCCCTG GACACGCCCC CGACCTCCGT GACAGGCACC
    TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC
    AGCAGGGCTG GCACCCGCCG CCTCCCGGGC AGCCCAAGGC AAGCACAGGC AACCGGGGCC
    GGGCCACGGC ACCTGGGGGT GGAGCCGCTC GTACGGGCAT CTCGAGCTAA TCTGGTGGGC
    GCAAGCTGGG GGTCAGAGGA TAGCCTTTCG GTGGCCAGTG ACCTGTACGG CAGCGCATTC
    AGCCTGTACA GAGGACGGGC GCTCTCGATC CACGTCAGTG TCCCTCAGAG CGGACTGCAC
    AAGGAGGAGC CTGACCTTCA GCCTCAGCCC GCCAGCGAAG CCCCCCGTCG CCCAGCCCTG
    CCGCCCCCCT CCAAATCCGC GCTGCTGCCC CCACCGTCCC CTCGGGTGGG TAAGCGGCCC
    CCGCCGGGAG CCGGCGGCCA GCCCCTGGCC ACCCCCACGC CGCCGCACCG GCGCTCTCGG
    GAGCCGGTGC CGCCCGAGGA CGCCACCGCC GAAGAAAAGC GAGGGAAAAA GTCCAAGTCG
    TCCGGGCCCT CGCTAGCGGG CACGGCGGAG TCCCGGCCCC AGACGCCCCT GAGTGAGGCC
    TCGGGCCGCC AGTCGGCGCT GGGCCGCTCC CCGAGGCTGG TGCGCGCTGG CTCCCGCATC
    CTGGACAAGC TGCAGTTCTT CGAGGAGCGC CGGCGCAGCC TGGAGCGCAG CGACTCGCCG
    CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG CGCAAGGCCC GTTCGCTGGA GCAGCCCAAG
    TCCGAGGGCG GCGGGCCGTG GGGCACGCCC AGGGCCTCGC AGGAGGAGCT GCGGTCCCCG
    GCGGGCAGCG TGGCCGAGCG GCGCCGCCTA TTCCAACAGA AGGCGGCCTC GCTGGACGAG
    CGCACGCGTC AGCGCAGCCC TGCGTCCGAC CTCGAGCTGC GCTTCGCCCA GGAGCTGGGC
    CGCATCCGCC GCTCCACGTC GCGGGAGGAG CTGGTGCGCT CTCACGAGTC CCTGCGCGCC
    ACGCTGCAGC GCGCCCCGTC CCCACGAGAG CCCGGCGAGC CGCCGCTCTT CTCCCAGCCC
    TCCACCCCCA AGACCCCGCG GGCCTTGAGC CCCGCCGCCG CCCAGCCGCC CCCTGCGAGC
    GTCGCGGAAA AGCCCGGGGA TGAGCCAGGG AGGCCCCGGG GCCGTGGGCC AGCGGGCAGG
    ACGGAGCCGG GGGAAGGCCC GCAGCAGGAG GTTAGGCGCC GGGACCAGTT CCCGCTGACC
    CGGAGCAGAG CCATCCAGGA GTGCCGGAGC CCGGTGCCGC CCCCCACCTC TGAGCCCCCC
    GAGACCAGGA CGAAAGCTCC CCCGGGTCGG AAGCGGGAGC CCCCGCCGCA GGCCGTGCGC
    TTCCTGCCCT GGGCCACGCC GGGCCAGGAG GGCGCTGCTG TGCCCCAGAC CTTGGAGAAG
    AACAGGGCGG GACCCGAGGC CGAGAAGAGG CTGCGCAGAG GGCCAGAGGA GGACGGTCCT
    TGGGGGGCCT GGGACCGCCG AGGGGCCCGC AGCCAGGGCA GAGGTCGCCG GGCGCGGCCC
    ACCTCGCCTG AGCTCGAGTC TTCGGATGAC TCCTATGTGT CTGCTGGAGA AGAGCCTCTG
    GAGGCCCCTG TGTTTGAGAT CCCCCTGCAA AATGCAGTGG TGGCCCCAGG GGTGGACGTG
    CTGCTCAAGT GTATTGTTAC CGCCAACCCC CCGCCCCAAG TGTCCTGGCA GAAGGATGGG
    TCCCCGCTGC GCAGTGATGG CCGCCTTCTC ATCCGGGCAG AAGGCGAGCG GCACACCCTG
    CTGCTCCGGG AGGCCCGGGC CGCCGATGCC GGGAGCTATG CAGCCACTGC CACCAACGAG
    CTGGGCCAGG CCCGCTGCGC TGCCACGCTG GCCGTGAGAC CTGGTGGGTC AACATCTCCT
    TTCAGCAGTC CTATCACCTC TGACGAGGAG TATCTGAGCC CCCCAGAGGA GTTCCCGGAA
    CCCGGGGAGA CCTGGCCCCG AGCCCCCACC ATGAAGCTCA GTCCTGGCCA GAATCGCCGT
    TCCTCCGACA CTGGCTCCAA GGCGCCCCCT ACTTTCAAGG TTTCACTTAT GGACCAGTCA
    GTAAGGGAAG GCCAAGACGT CACCATGAGC ATCCGTGTGC AGGGCGAACC CAAGCCGGTG
    GTCTCCTGGC TGAGAAACCG TCAGCCTGTG CGCCCAGACC AGCGGCGCTT TGCTGAGGAG
    GCAGAGGATG GGCTCTGCCG GCTTCGGATC CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC
    TACACCTGCA AGGCGGTTAA CGAGTATGGC GCTCGACAGT GTGAGGCGCG CCTGGAGGTC
    AGAGCACACC CTGAAAGCCG GTCCCTGGCC GTGCTGGCCC CTCTGCAGGA CGTGGACGTG
    GGGGCCGGGG AGATGGCGCT GTTTGAGTGC CTGGTGGCGG GTCCCACCGA CGTGGAGGTA
    GACTGGCTGT GCCGAGGCCG CCTGCTGCAG CCTGCACTAC TCAAATGCAA GATGCATTTC
    GATGGCCGCA AATGCAAGCT GCTGCTCACC TCTGTGCATG AGGACGACAG CGGCGTCTAC
    ACCTGCAAGC TCAGCACGGC CAAAGATGAA CTGACCTGCA GTGCCCGGCT GACCGTGCGA
    CCCTCGCTGG CACCCCTGTT CACGCGGCTG CTGGAGGACG TGGAAGTGCT GGAGGGCCGC
    GCTGCCCGCT TCGACTGCAA GATCAGCGGG AGCCCGCCCC CCTCCGTTAC CTGGACTCAC
    TTTGGCCACC CCGTGGAGGA GAGCGAGAAC GTGCGGCTGC GGCAGGACGG GGGCCTGCAT
    TCGCTGCACA TCGCACATGT GGGCAGTGAG GATGAGGGGC TGTACGCGGT CAGTGCTGCC
    AACACCCACG GCCAGGCCCA CTGCTCAGCC CAGCTCTACG TGGAGGAGCC TCGGACGGCT
    GCCACCGGCC CTAGCTCCAA GCTGGAGAAG ATGCCCTCCA TCCCCGAGGA GCCCGAGCAG
    GGTGAGCTGG AGCGGCTGTC CATGCCCGAC TTCCTGCGGC CGCTGCAGGA TCTGGACGTG
    GGACTGGCCA AGGAGGCCAT GCTGGAGTGC CAGGTGACCG GCCTGCCCTA CCCCACCATC
    AGCTGGTTCC ACAACGGCCA CCGCATCCAG AGCAGCGACG ACCGACGCAT GACGCAGTAC
    AGGGATGTCC ATCGCTTGGT GTTCCCTGCC GTGGGACCTC AGCATGCTGG TGTCTACAAG
    AGCGTCATTG CTAACAAGCT GGGCAAAGCC GCCTGCTATG CTCATCTCTA CGTCACAGAT
    GTGGTTCCAG GCCCCCCAGA CGGTGCCCCT CAGGTAGTGG CTGTGACCGG GAGGATGATC
    ACGCTTGCAT GGAACCCCCC GAGGAGTCTG GACATGGCTA TCGACCCAGA CGCCCTGACC
    TACACCGTGC AGCACCAGGT GCTGGGCTCA GACCAGTGGA CGGCGCTGGT CACGGGCCTG
    CGGCAGCCCG GGTGGGCAGC CACCGGATTG CGAAAGGGGG TCCAGCACGT CTTCCGGGTT
    CTCAGCACCA CCATCAAGAG CAGCAGCAAG CCCTCGCCCC CGTCCGAGCC CACGCAGCTG
    CTGGAGCGTG GCCCGCCCCT GGAGGAGGCG CCCGCTGTGC TGGACAAGCC AGACGTCGTG
    TATGTGGTGG AGGGCCAGCC CACCAGTGTC ACCGTCACCT TCAACCACGT GGAGGCCCAG
    GTTGTCTGGA GGAGCTGCCG AGGAGCCCTC CTAGAGGCCC GGCCAGGTGT GTTTGAGCTG
    AGCCAGCCAG ATGACGACCA GTACTCTCTT AGGATCTGCC GGGTGAGCCG CCGGGACCTG
    GGAGCCCTCA CTTGCACTGC CCGCAACCGC CACGGCACAC AGGCCTGCTC AGTCACCCTG
    GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC ATCATGGAGG ATGTGGAGGT AGGGGCTGGA
    GAGACTGCTC GCTTCGCCGT GGTGGTGGAC GGGAAGCCCT TGCCGGACAT CATGTGGTAC
    AAGGATGAGG CGCTGCTGAC CGAGAGCAAC CATGTGAGCT TCGTGTACGA GGAGAACGAG
    TGCTCCCTGG TGGTGCTCAG CGCGGGGGCC CAGGATGGAG GGGTCTACAC CTGCACGGCC
    CGTAACCTGG CTGGCGAAGT CTCCTGCAAA GCAGAGCTGG CTGTGCACTC AGCTCAGACA
    GCTATGGAGG TGGAAGGGAT CGGGGAAGAC GAGGAGCAGC GAGGAAGGAG GCTCAGCGAC
    TTCTATGATA TTCAGCAGGA GATCGGCAGG GGTGCCTTCT CCTACCTGCG CCGTGTAGTG
    GAGAGGAGCT CCGGACTGGA GTTTGCAGCC AAGTTCATCC CCAGCCAGGC AAAGCCAAAG
    GCATCGGCGT GGCGGGAGGC ACGGCTGCTG GCCCGGCTCC AGCATGACTG CATCCTCTAC
    TTCCATGAGG CCTTCGAGAG GCGCCGGGGA CTGGTCATCG TCACCGAGCT CTGCACAGAA
    GAGCTTCTGG AGCGAATGGC CAGGAAACCC ACCGTGTGTG AGTCTGAGAT CCGGGCCTAC
    ATGCGGCAGG TACTAGAAGG AATATGCTAC TTGCACGAGA ACCACGTGCT GCACCTGGAT
    GTCAAGCCCG AGAACCTGCT GGTGTGGGAT GGTGTTGAGG GCGAGGAGCA GGTGAGGATC
    TGTGACTTTG GGAACGCTCA AGAGCTGACA CCGGGAGAGC CCCAGTATTG CCAATACGGC
    ACACCTGAGT TCGTGGCGCC CGAGATTGTC AACCAGACCC CTGTGTCTGG AGTCACTGAC
    ATCTGGCCCG TGGGTGTCGT CGCCTTCCTC TGCCTGACCG GAATCTCCCC GTTCGTTGGG
    GAAAATGATC GGACGACATT GATGAACATC CGAAACTACA ACGTGGCCTT CGAGGAGACC
    ACGTTCCTGA GCCTGAGCAG GGAGGCCCGG GGCTTCCTCA TCAAAGTGCT GGTGCGGGAC
    CGGCTGAGGC CTACTGCAGA GGAGACCCTG GAACATCCTT GGTTTAAAGC ACAGGCAAAG
    GGTGCAGTGG TGAGCACGGA TCACCTAAAG CTATTCCTCT CCCGGCGGAG GTGGCAGCGC
    TCCCAGATCA GCTACAAGTG CCACCTGGTG CTGCGCCCCA TCCCGGAGCT GCTGCGAGCA
    CCCCCGGAGC GGGTGTGGGT GGCCGTGCCC CGAAGACCAC CCCCTAGTGG AGGGCTGTCG
    TCATCATCCG ACTCTGAAGA GGAAGAGCTG GAGGAGCTGC CCTCTGTGCC CCGACCTCTG
    CAGCCTGAGT TTTCGGGCTC CCGGGTGTCC CTCACTGACA TTCCCACTGA GGATGAGGCC
    CTCGGGGCCC TGGAGGCTGG GGCGGCCACC CCCATGGACT GGCAAGAACA AGGAAAGGCC
    CCCTCTCAGG ACCAGGAGGC CCCCAGCCCT CAGGCACTCC CCTCCCCAGG CCAGGAGTCC
    CCGTCCGGGC CCAGCCCCCG GCGGGCAGAG CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC
    CTGCCCCGGG CCGGGCCGAG GGAACCGGGC CGGGGCCTGC ACAAGGCAGC GTCCGTCGAG
    CTGCCGCAGC GCCGAAGCCC CAGCCCGGGG GCCACCCGCC TGACCCGAGG AGGCCTGGGT
    GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC CTGCGCCAGC GGCTGCTGAG GGGTGGCCCC
    GATGATGGCA AGGTCAGCGG CCTCAGGGGC CCCCTGCTGG AGAGCCTGGG GGGCCGGGCT
    CGTGACCCCC GGCTGGCGCG GGCAGCCTCC AGCGAGGCAG CACCCCATCA CCAGCACCCA
    CCTGAGGCTC GGGGCCTGCA GAAGAGCAGT AGCTTCTCGC AGGGTGAGGC AGAGCCCCGG
    GGCCGGCACC GCCGAGCAGG GGCACCCCTT GAGATCCCCG TGGCCCGGCT GGGGGCCCGC
    AAGCTGCAGG AGTCTCCCTC CTTGTCCGCC CTCAGCGAGG CCCAACCACC TAGCCCCGTG
    CGGCCCAGTG CAGCCAAGCC CAGCACCCCT AAGTCTTCAG AACCTCTTGT GGGCACCCCC
    AGTGAGGCTG CACAGCCTCC AGCACCCCAG CCTGCCCGAG AGAAGGCCCC AGAATCTACG
    CCAGAACCAG CTCGGGGCCC CAAGCCCACA CAGCCCCCCA CAGCTCTGCA AACCCTAGCG
    CCTCCTCTCA CTCCGTATGC GCAGATCATG CAGTCGCTCC AGCTGCCAGG CCAGGCGCAA
    GGCCCCCCAC AGGGCGCAGT CGTATCTCCT ACATCTCGGT CTCCAGCATC TCGGTTGGAA
    CCCAAGCCCC ACCCGGCTGT GTTCTCCAGG GTGGCCTCCC CACCTCCAGG AGCCTCCGAG
    AAGCGCCTGC TGCCGGGAGC CGGAGCAACC CCAGGGCCAG CCGAAAAAGC CCGGGTCCCG
    GCGGTCCCCC GCAGGCCAGG GAGCAGTCTC AGCAGCAGCA TCGAGAACCT GGAGTCGGAG
    GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC CGGGAGTCGC CCCTGTCGCG GGGGCTGCGG
    CTGCTGAGCC GCTCGCGGTC GGAGGAGCGC GGCCCCTTCC GCGGGGCTGA GGAGGAGGAC
    GGCATTTACC GGCCCAGCCC GGCGGGCACG CCGCTGGAGC TGGTGCGACG GCCCGAGCGC
    TCGCGCTCCG TGCAGGACCT CAGGGCGGTC GGGGAGCCAG GCCTCGTGCG CCGCCTCTCG
    CTGTCGCTGT CCCAGCGTCT GCGGCGGACC CCGCCCGCGC AGCGCCACCC GGCCTGGGAG
    CCCCGCGGCG GGGACGGCGA GAGCTCGGAG GGCGGCAGCT CGGCGCGGGG CTCCCCGGTG
    CTGACGGTGC GCCGGAGGCT CAGCTCCACG CTGGAGCGGC TGTCGAGCCG GTTGCAGCGC
    AGCGGCAGCA GCGAGGACTC GGGGGGCGCG TCGGGCCGCA GCACGCCGCT GTTCGGCCGG
    CTGCGCAGGG CCACGTCCGA GAGCGAGAGT CTGCGGCGCC TGGACCTCGC GCAAAATCAG
    CTGGCGGCCC AAGCCGGCGC CGCCACCCCT TCCGCGGAGT CCCTGGGCTC CGAGGCCAGC
    GCTACGTCGG GCTCCTCTGC TCCTGCAGAA AGTCGAAGCC GCCTGCGCTG GGGCCTCTCT
    CGGCTGCGGA AGGACAAGAG GTTGTCACAG CCTAACCTCT CTGGCAGTGT CCAGGAGGAT
    TTGGGTCACC AGTATGTGCG CAGTGAGTCA GACTTTCCCC CAGTCTTCCA CATCAAACTC
    AAGGACCAGG TGCTGCTGGA GGGAGAGGCA GCCACCCTGC TCTGCTTGCC AGCAGCCTGC
    CCTGCACCCC ACATCTCCTG GATGAAAGAC AAGCAGTCCC TGCGGTCAGA GCCCTCGGTG
    ATCATCGTGT CCTGCAAAGA TGGTCGTCAG CTACTGAGCA TCCCCCGGGC GGGCAAGCGG
    CATGCGGGGC TCTATGAGTG CTCCGCCACC AACGTCCTTG GCAGCATCAC CAGCTCCTGT
    ACCGTGGCTG TGGCTCGTAC CCCAGGAAAG CTAGCTCCTC CTGAGGTGCC CCAGACCTAC
    CAGGACACAG CACTGGTGCT CTGGAAGCCG GGAGACAGCA AGGCGCCTTG CACATACACG
    CTGGAGCGGC GGGTGGATGG GGAGTCTTCC TGGCACCCCG TGAGCTCGGG CATCCCCGAC
    TGCTACTACA ACGTGACCCA CCTACCAGTC GGCATGGCTG TGAGGTTCCG TGTGGCCTGT
    GCCAACCGTG CTGGGCAAGG GCCTTTCAGC AACCCTTCTG AGAAGGTCCT CGTCAGGGGC
    GTGCAAGATT CCTCAGCTCT GCCATCTGCT GCTCACAGAG ATGCCCCTGT TACCTCAGGG
    CCAGCCAGGG CCCCGCCTCC TGACGCTCCT ACCTCACTGG CCCCTCCCCT GAGCCCCTCC
    CCAGCTCCTG CTCCCCCAGG CCCCCAGGCA GCCCCTCTCG GCCCTTCATC TTCCCCAGCA
    CCCCCCAGCC AGGCCTTGTC CTCCCTCAAG GCTGTGGGTC CACCACCCCA AACCCCCCCA
    CGAAAGCACC GGGGCCTGCA GGCTGCCCAG CAAGTAGAAC CTACCCCACC CAGTGCCCAG
    GACTCCCCAA GTGAGCCCAA GTCTTCCGTC CCTGACACTG GGACCCCCAT CCCAGCCTCT
    GCCCCTCAAG TGGTGAAAAC AGCCTCTTCC TCAATGCCCC TGTATATGGT GACCTCCTTT
    GTGTCTGCAC CACCAGCCCC TGAGCCCCCA GCCCCCGAGC CCCCTCCTGA GCCCACCAAA
    GTGACTGTGC GGAGCCTCAG CCCAGCCAAG GAGGTGGTTG GCTCCCCTGG GGGTGGTCCC
    CCCAGGGCTC CTAAGCCAGA GGGCACCACT CTTCGACAGG GCCCTCCTCA GAAACCATAC
    ACCTTCCTGG AGGAGAAGGC CAGGGGTCGC TTTGGTGTGG TGCGTGCCTG CCGGGAGAAT
    GCCACCGGGC GAACATTCGT GGCCAAGATT GTGCCATACG CGGCCGAGGG CAAGCGGCAG
    GTCCTGCAGG AGTACGAGGT GCTGAGGACC CTGCACCACG AGCGGCTTAT GGCCCTGCAC
    GAAGCCTACA TCACCCCGCG CTACCTCGTG CTCATCGCCG AGAGCTGCGG CAACCGGGAG
    CTTCTCTGTG GGCTCAGCGA CAGATTCCGG TATTCAGAGG ATGATGTGGC CACCTATGTG
    GTGCAGCTGC TACAAGGCCT GGATTACCTC CATGGCCGCC ACGTGCTGCA CCTGGACATC
    AAGCCAGACA ACCTGCTGCT GGCCCCTGAC AACGCCCTCA AGATCGTGGA CTTTGGCAGT
    GCCCAGCCCT ACAACCCCCA GGCTCTGCGG CCCCTGGGTC ACCGCACGGG CACACTGGAG
    TTCATGGCTC CTGAGATGGT AAAGGGAGAC CCCATCGGCT CTGCCACGGA CATCTGGGGA
    GCGGGTGTGC TCACTTACAT CATGCTCAGT GGACGCTCCC CATTCTATGA GCCAGACCCC
    CAGGAAACAG AGGCTCGCAT TGTGGGGGGC CGCTTTGATG CCTTCCAGCT GTATCCCAAC
    ACATCCCAGA GTGCCACCCT CTTCTTGCGA AAGGTCCTCT CAGTACATCC CTGGAGCCGG
    CCCTCCGTGC AGGACTGCCT GGCTCACCCG TGGCTGCAGG ATGCCTACCT GATGAAACTG
    CGCCGCCAGA CACTCACCTT CACCACCAAC CGGCTCAAGG AGTTCCTGGG TGAGCAGCGG
    CGCCGCAGGG CCGAGGCCGC CACCCGCCAC AAGGTGCTGC TCCGCTCTTA CCCAGGCAGC
    CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16713
    Clone ID Related Accession (Same CDS sequence) XM_015459169.1 , XM_005202848.3 , XM_005202849.3
    Accession Version XM_015459169.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product striated muscle preferentially expressed protein kinase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103849.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAAGCTCA GTCCTGGCCA GAATCGCCGT TCCTCCGACA CTGGCTCCAA GGCGCCCCCT 
    ACTTTCAAGG TTTCACTTAT GGACCAGTCA GTAAGGGAAG GCCAAGACGT CACCATGAGC
    ATCCGTGTGC AGGGCGAACC CAAGCCGGTG GTCTCCTGGC TGAGAAACCG TCAGCCTGTG
    CGCCCAGACC AGCGGCGCTT TGCTGAGGAG GCAGAGGATG GGCTCTGCCG GCTTCGGATC
    CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC TACACCTGCA AGGCGGTTAA CGAGTATGGC
    GCTCGACAGT GTGAGGCGCG CCTGGAGGTC AGAGCACACC CTGAAAGCCG GTCCCTGGCC
    GTGCTGGCCC CTCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC
    CTGGTGGCGG GTCCCACCGA CGTGGAGGTA GACTGGCTGT GCCGAGGCCG CCTGCTGCAG
    CCTGCACTAC TCAAATGCAA GATGCATTTC GATGGCCGCA AATGCAAGCT GCTGCTCACC
    TCTGTGCATG AGGACGACAG CGGCGTCTAC ACCTGCAAGC TCAGCACGGC CAAAGATGAA
    CTGACCTGCA GTGCCCGGCT GACCGTGCGA CCCTCGCTGG CACCCCTGTT CACGCGGCTG
    CTGGAGGACG TGGAAGTGCT GGAGGGCCGC GCTGCCCGCT TCGACTGCAA GATCAGCGGG
    AGCCCGCCCC CCTCCGTTAC CTGGACTCAC TTTGGCCACC CCGTGGAGGA GAGCGAGAAC
    GTGCGGCTGC GGCAGGACGG GGGCCTGCAT TCGCTGCACA TCGCACATGT GGGCAGTGAG
    GATGAGGGGC TGTACGCGGT CAGTGCTGCC AACACCCACG GCCAGGCCCA CTGCTCAGCC
    CAGCTCTACG TGGAGGAGCC TCGGACGGCT GCCACCGGCC CTAGCTCCAA GCTGGAGAAG
    ATGCCCTCCA TCCCCGAGGA GCCCGAGCAG GGTGAGCTGG AGCGGCTGTC CATGCCCGAC
    TTCCTGCGGC CGCTGCAGGA TCTGGACGTG GGACTGGCCA AGGAGGCCAT GCTGGAGTGC
    CAGGTGACCG GCCTGCCCTA CCCCACCATC AGCTGGTTCC ACAACGGCCA CCGCATCCAG
    AGCAGCGACG ACCGACGCAT GACGCAGTAC AGGGATGTCC ATCGCTTGGT GTTCCCTGCC
    GTGGGACCTC AGCATGCTGG TGTCTACAAG AGCGTCATTG CTAACAAGCT GGGCAAAGCC
    GCCTGCTATG CTCATCTCTA CGTCACAGAT GTGGTTCCAG GCCCCCCAGA CGGTGCCCCT
    CAGGTAGTGG CTGTGACCGG GAGGATGATC ACGCTTGCAT GGAACCCCCC GAGGAGTCTG
    GACATGGCTA TCGACCCAGA CGCCCTGACC TACACCGTGC AGCACCAGGT GCTGGGCTCA
    GACCAGTGGA CGGCGCTGGT CACGGGCCTG CGGCAGCCCG GGTGGGCAGC CACCGGATTG
    CGAAAGGGGG TCCAGCACGT CTTCCGGGTT CTCAGCACCA CCATCAAGAG CAGCAGCAAG
    CCCTCGCCCC CGTCCGAGCC CACGCAGCTG CTGGAGCGTG GCCCGCCCCT GGAGGAGGCG
    CCCGCTGTGC TGGACAAGCC AGACGTCGTG TATGTGGTGG AGGGCCAGCC CACCAGTGTC
    ACCGTCACCT TCAACCACGT GGAGGCCCAG GTTGTCTGGA GGAGCTGCCG AGGAGCCCTC
    CTAGAGGCCC GGCCAGGTGT GTTTGAGCTG AGCCAGCCAG ATGACGACCA GTACTCTCTT
    AGGATCTGCC GGGTGAGCCG CCGGGACCTG GGAGCCCTCA CTTGCACTGC CCGCAACCGC
    CACGGCACAC AGGCCTGCTC AGTCACCCTG GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC
    ATCATGGAGG ATGTGGAGGT AGGGGCTGGA GAGACTGCTC GCTTCGCCGT GGTGGTGGAC
    GGGAAGCCCT TGCCGGACAT CATGTGGTAC AAGGATGAGG CGCTGCTGAC CGAGAGCAAC
    CATGTGAGCT TCGTGTACGA GGAGAACGAG TGCTCCCTGG TGGTGCTCAG CGCGGGGGCC
    CAGGATGGAG GGGTCTACAC CTGCACGGCC CGTAACCTGG CTGGCGAAGT CTCCTGCAAA
    GCAGAGCTGG CTGTGCACTC AGCTCAGACA GCTATGGAGG TGGAAGGGAT CGGGGAAGAC
    GAGGAGCAGC GAGGAAGGAG GCTCAGCGAC TTCTATGATA TTCAGCAGGA GATCGGCAGG
    GGTGCCTTCT CCTACCTGCG CCGTGTAGTG GAGAGGAGCT CCGGACTGGA GTTTGCAGCC
    AAGTTCATCC CCAGCCAGGC AAAGCCAAAG GCATCGGCAT GGCGGGAGGC ACGGCTGCTG
    GCCCGGCTCC AGCATGACTG CATCCTCTAC TTCCATGAGG CCTTCGAGAG GCGCCGGGGA
    CTGGTCATCG TCACCGAGCT CTGCACAGAA GAGCTTCTGG AGCGAATGGC CAGGAAACCC
    ACCGTGTGTG AGTCTGAGAT CCGGGCCTAC ATGCGGCAGG TACTAGAAGG AATATGCTAC
    TTGCACGAGA ACCACGTGCT GCACCTGGAT GTCAAGCCCG AGAACCTGCT GGTGTGGGAT
    GGTGTTGAGG GCGAGGAGCA GGTGAGGATC TGTGACTTTG GGAACGCTCA AGAGCTGACA
    CCGGGAGAGC CCCAGTATTG CCAATACGGC ACACCTGAGT TCGTGGCGCC CGAGATTGTC
    AACCAGACCC CTGTGTCTGG AGTCACTGAC ATCTGGCCCG TGGGTGTCGT CGCCTTCCTC
    TGCCTGACCG GAATCTCCCC GTTCGTTGGG GAAAATGATC GGACGACATT GATGAACATC
    CGAAACTACA ACGTGGCCTT CGAGGAGACC ACGTTCCTGA GCCTGAGCAG GGAGGCCCGG
    GGCTTCCTCA TCAAAGTGCT GGTGCGGGAC CGGCTGAGGC CTACTGCAGA GGAGACCCTG
    GAACATCCTT GGTTTAAAGC ACAGGCAAAG GGTGCAGTGG TGAGCACGGA TCACCTAAAG
    CTATTCCTCT CCCGGCGGAG GTGGCAGCGC TCCCAGATCA GCTACAAGTG CCACCTGGTG
    CTGCGCCCCA TCCCGGAGCT GCTGCGAGCA CCCCCGGAGC GGGTGTGGGT GGCCGTGCCC
    CGAAGACCAC CCCCTAGTGG AGGGCTGTCG TCATCATCCG ACTCTGAAGA GGAAGAGCTG
    GAGGAGCTGC CCTCTGTGCC CCGACCTCTG CAGCCTGAGT TTTCGGGCTC CCGGGTGTCC
    CTCACTGACA TTCCCACTGA GGATGAGGCC CTCGGGGCCC TGGAGGCTGG GGCGGCCACC
    CCCATGGACT GGCAAGAACA AGGAAAGGCC CCCTCTCAGG ACCAGGAGGC CCCCAGCCCT
    CAGGCACTCC CCTCCCCAGG CCAGGAGTCC CCGTCCGGGC CCAGCCCCCG GCGGGCAGAG
    CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC CTGCCCCGGG CCGGGCCGAG GGAACCGGGC
    CGGGGCCTGC ACAAGGCAGC GTCCGTCGAG CTGCCGCAGC GCCGAAGCCC CAGCCCGGGG
    GCCACCCGCC TGACCCGAGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC
    CTGCGCCAGC GGCTGCTGAG GGGTGGCCCC GATGATGGCA AGGTCAGCGG CCTCAGGGGC
    CCCCTGCTGG AGAGCCTGGG GGGCCGGGCT CGTGACCCCC GGCTGGCGCG GGCAGCCTCC
    AGCGAGGCAG CACCCCATCA CCAGCACCCA CCTGAGGCTC GGGGCCTGCA GAAGAGCAGT
    AGCTTCTCGC AGGGTGAGGC AGAGCCCCGG GGCCGGCACC GCCGAGCAGG GGCACCCCTT
    GAGATCCCCG TGGCCCGGCT GGGGGCCCGC AAGCTGCAGG AGTCTCCCTC CTTGTCCGCC
    CTCAGCGAGG CCCAACCACC TAGCCCCGTG CGGCCCAGTG CAGCCAAGCC CAGCACCCCT
    AAGTCTTCAG AACCTCTTGT GGGCACCCCC AGTGAGGCTG CACAGCCTCC AGCACCCCAG
    CCTGCCCGAG AGAAGGCCCC AGAATCTACG CCAGAACCAG CTCGGGGCCC CAAGCCCACA
    CAGCCCCCCA CAGCTCTGCA AACCCTAGCG CCTCCTCTCA CTCCGTATGC GCAGATCATG
    CAGTCGCTCC AGCTGCCAGG CCAGGCGCAA GGCCCCCCAC AGGGCGCAGT CGTATCTCCT
    ACATCTCGGT CTCCAGCATC TCGGTTGGAA CCCAAGCCCC ACCCGGCTGT GTTCTCCAGG
    GTGGCCTCCC CACCTCCAGG AGCCTCCGAG AAGCGCCTGC TGCCGGGAGC CGGAGCAACC
    CCAGGGCCAG CCGAAAAAGC CCGGGTCCCG GCGGTCCCCC GCAGGCCAGG GAGCAGTCTC
    AGCAGCAGCA TCGAGAACCT GGAGTCGGAG GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC
    CGGGAGTCGC CCCTGTCGCG GGGGCTGCGG CTGCTGAGCC GCTCGCGGTC GGAGGAGCGC
    GGCCCCTTCC GCGGGGCTGA GGAGGAGGAC GGCATTTACC GGCCCAGCCC GGCGGGCACG
    CCGCTGGAGC TGGTGCGACG GCCCGAGCGC TCGCGCTCCG TGCAGGACCT CAGGGCGGTC
    GGGGAGCCAG GCCTCGTGCG CCGCCTCTCG CTGTCGCTGT CCCAGCGTCT GCGGCGGACC
    CCGCCCGCGC AGCGCCACCC GGCCTGGGAG CCCCGCGGCG GGGACGGCGA GAGCTCGGAG
    GGCGGCAGCT CGGCGCGGGG CTCCCCGGTG CTGACGGTGC GCCGGAGGCT CAGCTCCACG
    CTGGAGCGGC TGTCGAGCCG GTTGCAGCGC AGCGGCAGCA GCGAGGACTC GGGGGGCGCG
    TCGGGCCGCA GCACGCCGCT GTTCGGCCGG CTGCGCAGGG CCACGTCCGA GAGCGAGAGT
    CTGCGGCGCC TGGACCTCGC GCAAAATCAG CTGGCGGCCC AAGCCGGCGC CGCCACCCCT
    TCCGCGGAGT CCCTGGGCTC CGAGGCCAGC GCTACGTCGG GCTCCTCTGC TCCTGCAGAA
    AGTCGAAGCC GCCTGCGCTG GGGCCTCTCT CGGCTGCGGA AGGACAAGAG GTTGTCACAG
    CCTAACCTCT CTGGCAGTGT CCAGGAGGAT TTGGGTCACC AGTATGTGCG CAGTGAGTCA
    GACTTTCCCC CAGTCTTCCA CATCAAACTC AAGGACCAGG TGCTGCTGGA GGGAGAGGCA
    GCCACCCTGC TCTGCTTGCC GGCAGCCTGC CCTGCACCCC ACATCTCCTG GATGAAAGAC
    AAGCAGTCCC TGCGGTCAGA GCCCTCGGTG ATCATCGTGT CCTGCAAAGA TGGTCGTCAG
    CTACTGAGCA TCCCCCGGGC AGGCAAGCGG CATGCGGGGC TCTATGAGTG CTCCGCCACC
    AACGTCCTTG GCAGCATCAC CAGCTCCTGT ACCGTGGCTG TGGCTCGTAC CCCAGGAAAG
    CTAGCTCCTC CTGAGGTGCC CCAGACCTAC CAGGACACAG CACTGGTGCT CTGGAAGCCG
    GGAGACAGCA AGGCGCCTTG CACATACACG CTGGAGCGGC GGGTGGATGG GGAGTCTTCC
    TGGCACCCTG TGAGCTCGGG CATCCCCGAC TGCTACTACA ACGTGACCCA CCTACCAGTC
    GGCATGGCTG TGAGGTTCCG TGTGGCCTGT GCCAACCGTG CTGGGCAAGG GCCTTTCAGC
    AACCCTTCTG AGAAGGTCCT CGTCAGGGGC GTGCAAGATT CCTCAGCTCT GCCATCTGCT
    GCTCACAGAG ATGCCCCTGT TACCTCAGGG CCAGCCAGGG CCCCGCCTCC TGACGCTCCT
    ACCTCACTGG CCCCTCCCCT GAGCCCCTCC CCAGCTCCTG CTCCCCCAGG CCCCCAGGCA
    GCCCCTCTCG GCCCTTCATC TTCCCCAGCA CCCCCCAGCC AGGCCTTGTC CTCCCTCAAG
    GCTGTGGGTC CACCACCCCA AACCCCCCCA CGAAAGCACC GGGGCCTGCA GGCTGCCCAG
    CAAGTAGAAC CTACCCCACC CAGTGCCCAG GACTCCCCAA GTGAGCCCAA GTCTTCCGTC
    CCTGACACTG GGACCCCCAT CCCAGCCTCT GCCCCTCAAG TGGTGAAAAC AGCCTCTTCC
    TCAATGCCCC TGTATATGGT GACCTCCTTT GTGTCTGCAC CACCAGCCCC TGAGCCCCCA
    GCCCCCGAGC CCCCTCCTGA GCCCACCAAA GTGACTGTGC GGAGCCTCAG CCCAGCCAAG
    GAGGTGGTTG GCTCCCCTGG GGGTGGTCCC CCCAGGGCTC CTAAGCCAGA GGGCACCACT
    CTTCGACAGG GCCCTCCTCA GAAACCATAC ACCTTCCTGG AGGAGAAGGC CAGGGGTCGC
    TTTGGTGTGG TGCGTGCCTG CCGGGAGAAT GCCACCGGGC GAACATTCGT GGCCAAGATT
    GTGCCATACG CGGCCGAGGG CAAGCGGCAG GTCCTGCAGG AGTACGAGGT GCTGAGGACC
    CTGCACCACG AGCGGCTTAT GGCCCTGCAC GAAGCCTACA TCACCCCGCG CTACCTCGTG
    CTCATCGCCG AGAGCTGCGG CAACCGGGAG CTTCTCTGTG GGCTCAGCGA CAGATTCCGG
    TATTCAGAGG ATGATGTGGC CACCTATGTG GTGCAGCTGC TACAAGGCCT GGATTACCTC
    CATGGCCGCC ACGTGCTGCA CCTGGACATC AAGCCAGACA ACCTGCTGCT GGCCCCTGAC
    AACGCCCTCA AGATCGTGGA CTTTGGCAGT GCCCAGCCCT ACAACCCCCA GGCTCTGCGG
    CCCCTGGGTC ACCGCACGGG CACACTGGAG TTCATGGCTC CTGAGATGGT AAAGGGAGAC
    CCCATCGGCT CTGCCACGGA CATCTGGGGA GCGGGTGTGC TCACTTACAT CATGCTCAGT
    GGACGCTCCC CATTCTATGA GCCAGACCCC CAGGAAACAG AGGCTCGCAT TGTGGGGGGC
    CGCTTTGATG CCTTCCAGCT GTATCCCAAC ACATCCCAGA GTGCCACCCT CTTCTTGCGA
    AAGGTCCTCT CAGTACATCC CTGGAGCCGG CCCTCCGTGC AGGACTGCCT GGCTCACCCG
    TGGCTGCAGG ATGCCTACCT GATGAAACTG CGCCGCCAGA CACTCACCTT CACCACCAAC
    CGGCTCAAGG AGTTCCTGGG TGAGCAGCGG CGCCGCAGGG CCGAGGCCGC CACCCGCCAC
    AAGGTGCTGC TCCGCTCTTA CCCAGGCAGC CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314655.1
    CDS2200..9495
    Translation

    Target ORF information:

    RefSeq Version XM_015459169.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SPEG complex locus (SPEG), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459169.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAAGCTCA GTCCTGGCCA GAATCGCCGT TCCTCCGACA CTGGCTCCAA GGCGCCCCCT 
    ACTTTCAAGG TTTCACTTAT GGACCAGTCA GTAAGGGAAG GCCAAGACGT CACCATGAGC
    ATCCGTGTGC AGGGCGAACC CAAGCCGGTG GTCTCCTGGC TGAGAAACCG TCAGCCTGTG
    CGCCCAGACC AGCGGCGCTT TGCTGAGGAG GCAGAGGATG GGCTCTGCCG GCTTCGGATC
    CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC TACACCTGCA AGGCGGTTAA CGAGTATGGC
    GCTCGACAGT GTGAGGCGCG CCTGGAGGTC AGAGCACACC CTGAAAGCCG GTCCCTGGCC
    GTGCTGGCCC CTCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC
    CTGGTGGCGG GTCCCACCGA CGTGGAGGTA GACTGGCTGT GCCGAGGCCG CCTGCTGCAG
    CCTGCACTAC TCAAATGCAA GATGCATTTC GATGGCCGCA AATGCAAGCT GCTGCTCACC
    TCTGTGCATG AGGACGACAG CGGCGTCTAC ACCTGCAAGC TCAGCACGGC CAAAGATGAA
    CTGACCTGCA GTGCCCGGCT GACCGTGCGA CCCTCGCTGG CACCCCTGTT CACGCGGCTG
    CTGGAGGACG TGGAAGTGCT GGAGGGCCGC GCTGCCCGCT TCGACTGCAA GATCAGCGGG
    AGCCCGCCCC CCTCCGTTAC CTGGACTCAC TTTGGCCACC CCGTGGAGGA GAGCGAGAAC
    GTGCGGCTGC GGCAGGACGG GGGCCTGCAT TCGCTGCACA TCGCACATGT GGGCAGTGAG
    GATGAGGGGC TGTACGCGGT CAGTGCTGCC AACACCCACG GCCAGGCCCA CTGCTCAGCC
    CAGCTCTACG TGGAGGAGCC TCGGACGGCT GCCACCGGCC CTAGCTCCAA GCTGGAGAAG
    ATGCCCTCCA TCCCCGAGGA GCCCGAGCAG GGTGAGCTGG AGCGGCTGTC CATGCCCGAC
    TTCCTGCGGC CGCTGCAGGA TCTGGACGTG GGACTGGCCA AGGAGGCCAT GCTGGAGTGC
    CAGGTGACCG GCCTGCCCTA CCCCACCATC AGCTGGTTCC ACAACGGCCA CCGCATCCAG
    AGCAGCGACG ACCGACGCAT GACGCAGTAC AGGGATGTCC ATCGCTTGGT GTTCCCTGCC
    GTGGGACCTC AGCATGCTGG TGTCTACAAG AGCGTCATTG CTAACAAGCT GGGCAAAGCC
    GCCTGCTATG CTCATCTCTA CGTCACAGAT GTGGTTCCAG GCCCCCCAGA CGGTGCCCCT
    CAGGTAGTGG CTGTGACCGG GAGGATGATC ACGCTTGCAT GGAACCCCCC GAGGAGTCTG
    GACATGGCTA TCGACCCAGA CGCCCTGACC TACACCGTGC AGCACCAGGT GCTGGGCTCA
    GACCAGTGGA CGGCGCTGGT CACGGGCCTG CGGCAGCCCG GGTGGGCAGC CACCGGATTG
    CGAAAGGGGG TCCAGCACGT CTTCCGGGTT CTCAGCACCA CCATCAAGAG CAGCAGCAAG
    CCCTCGCCCC CGTCCGAGCC CACGCAGCTG CTGGAGCGTG GCCCGCCCCT GGAGGAGGCG
    CCCGCTGTGC TGGACAAGCC AGACGTCGTG TATGTGGTGG AGGGCCAGCC CACCAGTGTC
    ACCGTCACCT TCAACCACGT GGAGGCCCAG GTTGTCTGGA GGAGCTGCCG AGGAGCCCTC
    CTAGAGGCCC GGCCAGGTGT GTTTGAGCTG AGCCAGCCAG ATGACGACCA GTACTCTCTT
    AGGATCTGCC GGGTGAGCCG CCGGGACCTG GGAGCCCTCA CTTGCACTGC CCGCAACCGC
    CACGGCACAC AGGCCTGCTC AGTCACCCTG GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC
    ATCATGGAGG ATGTGGAGGT AGGGGCTGGA GAGACTGCTC GCTTCGCCGT GGTGGTGGAC
    GGGAAGCCCT TGCCGGACAT CATGTGGTAC AAGGATGAGG CGCTGCTGAC CGAGAGCAAC
    CATGTGAGCT TCGTGTACGA GGAGAACGAG TGCTCCCTGG TGGTGCTCAG CGCGGGGGCC
    CAGGATGGAG GGGTCTACAC CTGCACGGCC CGTAACCTGG CTGGCGAAGT CTCCTGCAAA
    GCAGAGCTGG CTGTGCACTC AGCTCAGACA GCTATGGAGG TGGAAGGGAT CGGGGAAGAC
    GAGGAGCAGC GAGGAAGGAG GCTCAGCGAC TTCTATGATA TTCAGCAGGA GATCGGCAGG
    GGTGCCTTCT CCTACCTGCG CCGTGTAGTG GAGAGGAGCT CCGGACTGGA GTTTGCAGCC
    AAGTTCATCC CCAGCCAGGC AAAGCCAAAG GCATCGGCAT GGCGGGAGGC ACGGCTGCTG
    GCCCGGCTCC AGCATGACTG CATCCTCTAC TTCCATGAGG CCTTCGAGAG GCGCCGGGGA
    CTGGTCATCG TCACCGAGCT CTGCACAGAA GAGCTTCTGG AGCGAATGGC CAGGAAACCC
    ACCGTGTGTG AGTCTGAGAT CCGGGCCTAC ATGCGGCAGG TACTAGAAGG AATATGCTAC
    TTGCACGAGA ACCACGTGCT GCACCTGGAT GTCAAGCCCG AGAACCTGCT GGTGTGGGAT
    GGTGTTGAGG GCGAGGAGCA GGTGAGGATC TGTGACTTTG GGAACGCTCA AGAGCTGACA
    CCGGGAGAGC CCCAGTATTG CCAATACGGC ACACCTGAGT TCGTGGCGCC CGAGATTGTC
    AACCAGACCC CTGTGTCTGG AGTCACTGAC ATCTGGCCCG TGGGTGTCGT CGCCTTCCTC
    TGCCTGACCG GAATCTCCCC GTTCGTTGGG GAAAATGATC GGACGACATT GATGAACATC
    CGAAACTACA ACGTGGCCTT CGAGGAGACC ACGTTCCTGA GCCTGAGCAG GGAGGCCCGG
    GGCTTCCTCA TCAAAGTGCT GGTGCGGGAC CGGCTGAGGC CTACTGCAGA GGAGACCCTG
    GAACATCCTT GGTTTAAAGC ACAGGCAAAG GGTGCAGTGG TGAGCACGGA TCACCTAAAG
    CTATTCCTCT CCCGGCGGAG GTGGCAGCGC TCCCAGATCA GCTACAAGTG CCACCTGGTG
    CTGCGCCCCA TCCCGGAGCT GCTGCGAGCA CCCCCGGAGC GGGTGTGGGT GGCCGTGCCC
    CGAAGACCAC CCCCTAGTGG AGGGCTGTCG TCATCATCCG ACTCTGAAGA GGAAGAGCTG
    GAGGAGCTGC CCTCTGTGCC CCGACCTCTG CAGCCTGAGT TTTCGGGCTC CCGGGTGTCC
    CTCACTGACA TTCCCACTGA GGATGAGGCC CTCGGGGCCC TGGAGGCTGG GGCGGCCACC
    CCCATGGACT GGCAAGAACA AGGAAAGGCC CCCTCTCAGG ACCAGGAGGC CCCCAGCCCT
    CAGGCACTCC CCTCCCCAGG CCAGGAGTCC CCGTCCGGGC CCAGCCCCCG GCGGGCAGAG
    CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC CTGCCCCGGG CCGGGCCGAG GGAACCGGGC
    CGGGGCCTGC ACAAGGCAGC GTCCGTCGAG CTGCCGCAGC GCCGAAGCCC CAGCCCGGGG
    GCCACCCGCC TGACCCGAGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC
    CTGCGCCAGC GGCTGCTGAG GGGTGGCCCC GATGATGGCA AGGTCAGCGG CCTCAGGGGC
    CCCCTGCTGG AGAGCCTGGG GGGCCGGGCT CGTGACCCCC GGCTGGCGCG GGCAGCCTCC
    AGCGAGGCAG CACCCCATCA CCAGCACCCA CCTGAGGCTC GGGGCCTGCA GAAGAGCAGT
    AGCTTCTCGC AGGGTGAGGC AGAGCCCCGG GGCCGGCACC GCCGAGCAGG GGCACCCCTT
    GAGATCCCCG TGGCCCGGCT GGGGGCCCGC AAGCTGCAGG AGTCTCCCTC CTTGTCCGCC
    CTCAGCGAGG CCCAACCACC TAGCCCCGTG CGGCCCAGTG CAGCCAAGCC CAGCACCCCT
    AAGTCTTCAG AACCTCTTGT GGGCACCCCC AGTGAGGCTG CACAGCCTCC AGCACCCCAG
    CCTGCCCGAG AGAAGGCCCC AGAATCTACG CCAGAACCAG CTCGGGGCCC CAAGCCCACA
    CAGCCCCCCA CAGCTCTGCA AACCCTAGCG CCTCCTCTCA CTCCGTATGC GCAGATCATG
    CAGTCGCTCC AGCTGCCAGG CCAGGCGCAA GGCCCCCCAC AGGGCGCAGT CGTATCTCCT
    ACATCTCGGT CTCCAGCATC TCGGTTGGAA CCCAAGCCCC ACCCGGCTGT GTTCTCCAGG
    GTGGCCTCCC CACCTCCAGG AGCCTCCGAG AAGCGCCTGC TGCCGGGAGC CGGAGCAACC
    CCAGGGCCAG CCGAAAAAGC CCGGGTCCCG GCGGTCCCCC GCAGGCCAGG GAGCAGTCTC
    AGCAGCAGCA TCGAGAACCT GGAGTCGGAG GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC
    CGGGAGTCGC CCCTGTCGCG GGGGCTGCGG CTGCTGAGCC GCTCGCGGTC GGAGGAGCGC
    GGCCCCTTCC GCGGGGCTGA GGAGGAGGAC GGCATTTACC GGCCCAGCCC GGCGGGCACG
    CCGCTGGAGC TGGTGCGACG GCCCGAGCGC TCGCGCTCCG TGCAGGACCT CAGGGCGGTC
    GGGGAGCCAG GCCTCGTGCG CCGCCTCTCG CTGTCGCTGT CCCAGCGTCT GCGGCGGACC
    CCGCCCGCGC AGCGCCACCC GGCCTGGGAG CCCCGCGGCG GGGACGGCGA GAGCTCGGAG
    GGCGGCAGCT CGGCGCGGGG CTCCCCGGTG CTGACGGTGC GCCGGAGGCT CAGCTCCACG
    CTGGAGCGGC TGTCGAGCCG GTTGCAGCGC AGCGGCAGCA GCGAGGACTC GGGGGGCGCG
    TCGGGCCGCA GCACGCCGCT GTTCGGCCGG CTGCGCAGGG CCACGTCCGA GAGCGAGAGT
    CTGCGGCGCC TGGACCTCGC GCAAAATCAG CTGGCGGCCC AAGCCGGCGC CGCCACCCCT
    TCCGCGGAGT CCCTGGGCTC CGAGGCCAGC GCTACGTCGG GCTCCTCTGC TCCTGCAGAA
    AGTCGAAGCC GCCTGCGCTG GGGCCTCTCT CGGCTGCGGA AGGACAAGAG GTTGTCACAG
    CCTAACCTCT CTGGCAGTGT CCAGGAGGAT TTGGGTCACC AGTATGTGCG CAGTGAGTCA
    GACTTTCCCC CAGTCTTCCA CATCAAACTC AAGGACCAGG TGCTGCTGGA GGGAGAGGCA
    GCCACCCTGC TCTGCTTGCC GGCAGCCTGC CCTGCACCCC ACATCTCCTG GATGAAAGAC
    AAGCAGTCCC TGCGGTCAGA GCCCTCGGTG ATCATCGTGT CCTGCAAAGA TGGTCGTCAG
    CTACTGAGCA TCCCCCGGGC AGGCAAGCGG CATGCGGGGC TCTATGAGTG CTCCGCCACC
    AACGTCCTTG GCAGCATCAC CAGCTCCTGT ACCGTGGCTG TGGCTCGTAC CCCAGGAAAG
    CTAGCTCCTC CTGAGGTGCC CCAGACCTAC CAGGACACAG CACTGGTGCT CTGGAAGCCG
    GGAGACAGCA AGGCGCCTTG CACATACACG CTGGAGCGGC GGGTGGATGG GGAGTCTTCC
    TGGCACCCTG TGAGCTCGGG CATCCCCGAC TGCTACTACA ACGTGACCCA CCTACCAGTC
    GGCATGGCTG TGAGGTTCCG TGTGGCCTGT GCCAACCGTG CTGGGCAAGG GCCTTTCAGC
    AACCCTTCTG AGAAGGTCCT CGTCAGGGGC GTGCAAGATT CCTCAGCTCT GCCATCTGCT
    GCTCACAGAG ATGCCCCTGT TACCTCAGGG CCAGCCAGGG CCCCGCCTCC TGACGCTCCT
    ACCTCACTGG CCCCTCCCCT GAGCCCCTCC CCAGCTCCTG CTCCCCCAGG CCCCCAGGCA
    GCCCCTCTCG GCCCTTCATC TTCCCCAGCA CCCCCCAGCC AGGCCTTGTC CTCCCTCAAG
    GCTGTGGGTC CACCACCCCA AACCCCCCCA CGAAAGCACC GGGGCCTGCA GGCTGCCCAG
    CAAGTAGAAC CTACCCCACC CAGTGCCCAG GACTCCCCAA GTGAGCCCAA GTCTTCCGTC
    CCTGACACTG GGACCCCCAT CCCAGCCTCT GCCCCTCAAG TGGTGAAAAC AGCCTCTTCC
    TCAATGCCCC TGTATATGGT GACCTCCTTT GTGTCTGCAC CACCAGCCCC TGAGCCCCCA
    GCCCCCGAGC CCCCTCCTGA GCCCACCAAA GTGACTGTGC GGAGCCTCAG CCCAGCCAAG
    GAGGTGGTTG GCTCCCCTGG GGGTGGTCCC CCCAGGGCTC CTAAGCCAGA GGGCACCACT
    CTTCGACAGG GCCCTCCTCA GAAACCATAC ACCTTCCTGG AGGAGAAGGC CAGGGGTCGC
    TTTGGTGTGG TGCGTGCCTG CCGGGAGAAT GCCACCGGGC GAACATTCGT GGCCAAGATT
    GTGCCATACG CGGCCGAGGG CAAGCGGCAG GTCCTGCAGG AGTACGAGGT GCTGAGGACC
    CTGCACCACG AGCGGCTTAT GGCCCTGCAC GAAGCCTACA TCACCCCGCG CTACCTCGTG
    CTCATCGCCG AGAGCTGCGG CAACCGGGAG CTTCTCTGTG GGCTCAGCGA CAGATTCCGG
    TATTCAGAGG ATGATGTGGC CACCTATGTG GTGCAGCTGC TACAAGGCCT GGATTACCTC
    CATGGCCGCC ACGTGCTGCA CCTGGACATC AAGCCAGACA ACCTGCTGCT GGCCCCTGAC
    AACGCCCTCA AGATCGTGGA CTTTGGCAGT GCCCAGCCCT ACAACCCCCA GGCTCTGCGG
    CCCCTGGGTC ACCGCACGGG CACACTGGAG TTCATGGCTC CTGAGATGGT AAAGGGAGAC
    CCCATCGGCT CTGCCACGGA CATCTGGGGA GCGGGTGTGC TCACTTACAT CATGCTCAGT
    GGACGCTCCC CATTCTATGA GCCAGACCCC CAGGAAACAG AGGCTCGCAT TGTGGGGGGC
    CGCTTTGATG CCTTCCAGCT GTATCCCAAC ACATCCCAGA GTGCCACCCT CTTCTTGCGA
    AAGGTCCTCT CAGTACATCC CTGGAGCCGG CCCTCCGTGC AGGACTGCCT GGCTCACCCG
    TGGCTGCAGG ATGCCTACCT GATGAAACTG CGCCGCCAGA CACTCACCTT CACCACCAAC
    CGGCTCAAGG AGTTCCTGGG TGAGCAGCGG CGCCGCAGGG CCGAGGCCGC CACCCGCCAC
    AAGGTGCTGC TCCGCTCTTA CCCAGGCAGC CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16713
    Clone ID Related Accession (Same CDS sequence) XM_015459169.1 , XM_005202848.3 , XM_005202849.3
    Accession Version XM_005202848.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product striated muscle preferentially expressed protein kinase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103849.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202848.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAAGCTCA GTCCTGGCCA GAATCGCCGT TCCTCCGACA CTGGCTCCAA GGCGCCCCCT 
    ACTTTCAAGG TTTCACTTAT GGACCAGTCA GTAAGGGAAG GCCAAGACGT CACCATGAGC
    ATCCGTGTGC AGGGCGAACC CAAGCCGGTG GTCTCCTGGC TGAGAAACCG TCAGCCTGTG
    CGCCCAGACC AGCGGCGCTT TGCTGAGGAG GCAGAGGATG GGCTCTGCCG GCTTCGGATC
    CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC TACACCTGCA AGGCGGTTAA CGAGTATGGC
    GCTCGACAGT GTGAGGCGCG CCTGGAGGTC AGAGCACACC CTGAAAGCCG GTCCCTGGCC
    GTGCTGGCCC CTCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC
    CTGGTGGCGG GTCCCACCGA CGTGGAGGTA GACTGGCTGT GCCGAGGCCG CCTGCTGCAG
    CCTGCACTAC TCAAATGCAA GATGCATTTC GATGGCCGCA AATGCAAGCT GCTGCTCACC
    TCTGTGCATG AGGACGACAG CGGCGTCTAC ACCTGCAAGC TCAGCACGGC CAAAGATGAA
    CTGACCTGCA GTGCCCGGCT GACCGTGCGA CCCTCGCTGG CACCCCTGTT CACGCGGCTG
    CTGGAGGACG TGGAAGTGCT GGAGGGCCGC GCTGCCCGCT TCGACTGCAA GATCAGCGGG
    AGCCCGCCCC CCTCCGTTAC CTGGACTCAC TTTGGCCACC CCGTGGAGGA GAGCGAGAAC
    GTGCGGCTGC GGCAGGACGG GGGCCTGCAT TCGCTGCACA TCGCACATGT GGGCAGTGAG
    GATGAGGGGC TGTACGCGGT CAGTGCTGCC AACACCCACG GCCAGGCCCA CTGCTCAGCC
    CAGCTCTACG TGGAGGAGCC TCGGACGGCT GCCACCGGCC CTAGCTCCAA GCTGGAGAAG
    ATGCCCTCCA TCCCCGAGGA GCCCGAGCAG GGTGAGCTGG AGCGGCTGTC CATGCCCGAC
    TTCCTGCGGC CGCTGCAGGA TCTGGACGTG GGACTGGCCA AGGAGGCCAT GCTGGAGTGC
    CAGGTGACCG GCCTGCCCTA CCCCACCATC AGCTGGTTCC ACAACGGCCA CCGCATCCAG
    AGCAGCGACG ACCGACGCAT GACGCAGTAC AGGGATGTCC ATCGCTTGGT GTTCCCTGCC
    GTGGGACCTC AGCATGCTGG TGTCTACAAG AGCGTCATTG CTAACAAGCT GGGCAAAGCC
    GCCTGCTATG CTCATCTCTA CGTCACAGAT GTGGTTCCAG GCCCCCCAGA CGGTGCCCCT
    CAGGTAGTGG CTGTGACCGG GAGGATGATC ACGCTTGCAT GGAACCCCCC GAGGAGTCTG
    GACATGGCTA TCGACCCAGA CGCCCTGACC TACACCGTGC AGCACCAGGT GCTGGGCTCA
    GACCAGTGGA CGGCGCTGGT CACGGGCCTG CGGCAGCCCG GGTGGGCAGC CACCGGATTG
    CGAAAGGGGG TCCAGCACGT CTTCCGGGTT CTCAGCACCA CCATCAAGAG CAGCAGCAAG
    CCCTCGCCCC CGTCCGAGCC CACGCAGCTG CTGGAGCGTG GCCCGCCCCT GGAGGAGGCG
    CCCGCTGTGC TGGACAAGCC AGACGTCGTG TATGTGGTGG AGGGCCAGCC CACCAGTGTC
    ACCGTCACCT TCAACCACGT GGAGGCCCAG GTTGTCTGGA GGAGCTGCCG AGGAGCCCTC
    CTAGAGGCCC GGCCAGGTGT GTTTGAGCTG AGCCAGCCAG ATGACGACCA GTACTCTCTT
    AGGATCTGCC GGGTGAGCCG CCGGGACCTG GGAGCCCTCA CTTGCACTGC CCGCAACCGC
    CACGGCACAC AGGCCTGCTC AGTCACCCTG GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC
    ATCATGGAGG ATGTGGAGGT AGGGGCTGGA GAGACTGCTC GCTTCGCCGT GGTGGTGGAC
    GGGAAGCCCT TGCCGGACAT CATGTGGTAC AAGGATGAGG CGCTGCTGAC CGAGAGCAAC
    CATGTGAGCT TCGTGTACGA GGAGAACGAG TGCTCCCTGG TGGTGCTCAG CGCGGGGGCC
    CAGGATGGAG GGGTCTACAC CTGCACGGCC CGTAACCTGG CTGGCGAAGT CTCCTGCAAA
    GCAGAGCTGG CTGTGCACTC AGCTCAGACA GCTATGGAGG TGGAAGGGAT CGGGGAAGAC
    GAGGAGCAGC GAGGAAGGAG GCTCAGCGAC TTCTATGATA TTCAGCAGGA GATCGGCAGG
    GGTGCCTTCT CCTACCTGCG CCGTGTAGTG GAGAGGAGCT CCGGACTGGA GTTTGCAGCC
    AAGTTCATCC CCAGCCAGGC AAAGCCAAAG GCATCGGCAT GGCGGGAGGC ACGGCTGCTG
    GCCCGGCTCC AGCATGACTG CATCCTCTAC TTCCATGAGG CCTTCGAGAG GCGCCGGGGA
    CTGGTCATCG TCACCGAGCT CTGCACAGAA GAGCTTCTGG AGCGAATGGC CAGGAAACCC
    ACCGTGTGTG AGTCTGAGAT CCGGGCCTAC ATGCGGCAGG TACTAGAAGG AATATGCTAC
    TTGCACGAGA ACCACGTGCT GCACCTGGAT GTCAAGCCCG AGAACCTGCT GGTGTGGGAT
    GGTGTTGAGG GCGAGGAGCA GGTGAGGATC TGTGACTTTG GGAACGCTCA AGAGCTGACA
    CCGGGAGAGC CCCAGTATTG CCAATACGGC ACACCTGAGT TCGTGGCGCC CGAGATTGTC
    AACCAGACCC CTGTGTCTGG AGTCACTGAC ATCTGGCCCG TGGGTGTCGT CGCCTTCCTC
    TGCCTGACCG GAATCTCCCC GTTCGTTGGG GAAAATGATC GGACGACATT GATGAACATC
    CGAAACTACA ACGTGGCCTT CGAGGAGACC ACGTTCCTGA GCCTGAGCAG GGAGGCCCGG
    GGCTTCCTCA TCAAAGTGCT GGTGCGGGAC CGGCTGAGGC CTACTGCAGA GGAGACCCTG
    GAACATCCTT GGTTTAAAGC ACAGGCAAAG GGTGCAGTGG TGAGCACGGA TCACCTAAAG
    CTATTCCTCT CCCGGCGGAG GTGGCAGCGC TCCCAGATCA GCTACAAGTG CCACCTGGTG
    CTGCGCCCCA TCCCGGAGCT GCTGCGAGCA CCCCCGGAGC GGGTGTGGGT GGCCGTGCCC
    CGAAGACCAC CCCCTAGTGG AGGGCTGTCG TCATCATCCG ACTCTGAAGA GGAAGAGCTG
    GAGGAGCTGC CCTCTGTGCC CCGACCTCTG CAGCCTGAGT TTTCGGGCTC CCGGGTGTCC
    CTCACTGACA TTCCCACTGA GGATGAGGCC CTCGGGGCCC TGGAGGCTGG GGCGGCCACC
    CCCATGGACT GGCAAGAACA AGGAAAGGCC CCCTCTCAGG ACCAGGAGGC CCCCAGCCCT
    CAGGCACTCC CCTCCCCAGG CCAGGAGTCC CCGTCCGGGC CCAGCCCCCG GCGGGCAGAG
    CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC CTGCCCCGGG CCGGGCCGAG GGAACCGGGC
    CGGGGCCTGC ACAAGGCAGC GTCCGTCGAG CTGCCGCAGC GCCGAAGCCC CAGCCCGGGG
    GCCACCCGCC TGACCCGAGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC
    CTGCGCCAGC GGCTGCTGAG GGGTGGCCCC GATGATGGCA AGGTCAGCGG CCTCAGGGGC
    CCCCTGCTGG AGAGCCTGGG GGGCCGGGCT CGTGACCCCC GGCTGGCGCG GGCAGCCTCC
    AGCGAGGCAG CACCCCATCA CCAGCACCCA CCTGAGGCTC GGGGCCTGCA GAAGAGCAGT
    AGCTTCTCGC AGGGTGAGGC AGAGCCCCGG GGCCGGCACC GCCGAGCAGG GGCACCCCTT
    GAGATCCCCG TGGCCCGGCT GGGGGCCCGC AAGCTGCAGG AGTCTCCCTC CTTGTCCGCC
    CTCAGCGAGG CCCAACCACC TAGCCCCGTG CGGCCCAGTG CAGCCAAGCC CAGCACCCCT
    AAGTCTTCAG AACCTCTTGT GGGCACCCCC AGTGAGGCTG CACAGCCTCC AGCACCCCAG
    CCTGCCCGAG AGAAGGCCCC AGAATCTACG CCAGAACCAG CTCGGGGCCC CAAGCCCACA
    CAGCCCCCCA CAGCTCTGCA AACCCTAGCG CCTCCTCTCA CTCCGTATGC GCAGATCATG
    CAGTCGCTCC AGCTGCCAGG CCAGGCGCAA GGCCCCCCAC AGGGCGCAGT CGTATCTCCT
    ACATCTCGGT CTCCAGCATC TCGGTTGGAA CCCAAGCCCC ACCCGGCTGT GTTCTCCAGG
    GTGGCCTCCC CACCTCCAGG AGCCTCCGAG AAGCGCCTGC TGCCGGGAGC CGGAGCAACC
    CCAGGGCCAG CCGAAAAAGC CCGGGTCCCG GCGGTCCCCC GCAGGCCAGG GAGCAGTCTC
    AGCAGCAGCA TCGAGAACCT GGAGTCGGAG GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC
    CGGGAGTCGC CCCTGTCGCG GGGGCTGCGG CTGCTGAGCC GCTCGCGGTC GGAGGAGCGC
    GGCCCCTTCC GCGGGGCTGA GGAGGAGGAC GGCATTTACC GGCCCAGCCC GGCGGGCACG
    CCGCTGGAGC TGGTGCGACG GCCCGAGCGC TCGCGCTCCG TGCAGGACCT CAGGGCGGTC
    GGGGAGCCAG GCCTCGTGCG CCGCCTCTCG CTGTCGCTGT CCCAGCGTCT GCGGCGGACC
    CCGCCCGCGC AGCGCCACCC GGCCTGGGAG CCCCGCGGCG GGGACGGCGA GAGCTCGGAG
    GGCGGCAGCT CGGCGCGGGG CTCCCCGGTG CTGACGGTGC GCCGGAGGCT CAGCTCCACG
    CTGGAGCGGC TGTCGAGCCG GTTGCAGCGC AGCGGCAGCA GCGAGGACTC GGGGGGCGCG
    TCGGGCCGCA GCACGCCGCT GTTCGGCCGG CTGCGCAGGG CCACGTCCGA GAGCGAGAGT
    CTGCGGCGCC TGGACCTCGC GCAAAATCAG CTGGCGGCCC AAGCCGGCGC CGCCACCCCT
    TCCGCGGAGT CCCTGGGCTC CGAGGCCAGC GCTACGTCGG GCTCCTCTGC TCCTGCAGAA
    AGTCGAAGCC GCCTGCGCTG GGGCCTCTCT CGGCTGCGGA AGGACAAGAG GTTGTCACAG
    CCTAACCTCT CTGGCAGTGT CCAGGAGGAT TTGGGTCACC AGTATGTGCG CAGTGAGTCA
    GACTTTCCCC CAGTCTTCCA CATCAAACTC AAGGACCAGG TGCTGCTGGA GGGAGAGGCA
    GCCACCCTGC TCTGCTTGCC GGCAGCCTGC CCTGCACCCC ACATCTCCTG GATGAAAGAC
    AAGCAGTCCC TGCGGTCAGA GCCCTCGGTG ATCATCGTGT CCTGCAAAGA TGGTCGTCAG
    CTACTGAGCA TCCCCCGGGC AGGCAAGCGG CATGCGGGGC TCTATGAGTG CTCCGCCACC
    AACGTCCTTG GCAGCATCAC CAGCTCCTGT ACCGTGGCTG TGGCTCGTAC CCCAGGAAAG
    CTAGCTCCTC CTGAGGTGCC CCAGACCTAC CAGGACACAG CACTGGTGCT CTGGAAGCCG
    GGAGACAGCA AGGCGCCTTG CACATACACG CTGGAGCGGC GGGTGGATGG GGAGTCTTCC
    TGGCACCCTG TGAGCTCGGG CATCCCCGAC TGCTACTACA ACGTGACCCA CCTACCAGTC
    GGCATGGCTG TGAGGTTCCG TGTGGCCTGT GCCAACCGTG CTGGGCAAGG GCCTTTCAGC
    AACCCTTCTG AGAAGGTCCT CGTCAGGGGC GTGCAAGATT CCTCAGCTCT GCCATCTGCT
    GCTCACAGAG ATGCCCCTGT TACCTCAGGG CCAGCCAGGG CCCCGCCTCC TGACGCTCCT
    ACCTCACTGG CCCCTCCCCT GAGCCCCTCC CCAGCTCCTG CTCCCCCAGG CCCCCAGGCA
    GCCCCTCTCG GCCCTTCATC TTCCCCAGCA CCCCCCAGCC AGGCCTTGTC CTCCCTCAAG
    GCTGTGGGTC CACCACCCCA AACCCCCCCA CGAAAGCACC GGGGCCTGCA GGCTGCCCAG
    CAAGTAGAAC CTACCCCACC CAGTGCCCAG GACTCCCCAA GTGAGCCCAA GTCTTCCGTC
    CCTGACACTG GGACCCCCAT CCCAGCCTCT GCCCCTCAAG TGGTGAAAAC AGCCTCTTCC
    TCAATGCCCC TGTATATGGT GACCTCCTTT GTGTCTGCAC CACCAGCCCC TGAGCCCCCA
    GCCCCCGAGC CCCCTCCTGA GCCCACCAAA GTGACTGTGC GGAGCCTCAG CCCAGCCAAG
    GAGGTGGTTG GCTCCCCTGG GGGTGGTCCC CCCAGGGCTC CTAAGCCAGA GGGCACCACT
    CTTCGACAGG GCCCTCCTCA GAAACCATAC ACCTTCCTGG AGGAGAAGGC CAGGGGTCGC
    TTTGGTGTGG TGCGTGCCTG CCGGGAGAAT GCCACCGGGC GAACATTCGT GGCCAAGATT
    GTGCCATACG CGGCCGAGGG CAAGCGGCAG GTCCTGCAGG AGTACGAGGT GCTGAGGACC
    CTGCACCACG AGCGGCTTAT GGCCCTGCAC GAAGCCTACA TCACCCCGCG CTACCTCGTG
    CTCATCGCCG AGAGCTGCGG CAACCGGGAG CTTCTCTGTG GGCTCAGCGA CAGATTCCGG
    TATTCAGAGG ATGATGTGGC CACCTATGTG GTGCAGCTGC TACAAGGCCT GGATTACCTC
    CATGGCCGCC ACGTGCTGCA CCTGGACATC AAGCCAGACA ACCTGCTGCT GGCCCCTGAC
    AACGCCCTCA AGATCGTGGA CTTTGGCAGT GCCCAGCCCT ACAACCCCCA GGCTCTGCGG
    CCCCTGGGTC ACCGCACGGG CACACTGGAG TTCATGGCTC CTGAGATGGT AAAGGGAGAC
    CCCATCGGCT CTGCCACGGA CATCTGGGGA GCGGGTGTGC TCACTTACAT CATGCTCAGT
    GGACGCTCCC CATTCTATGA GCCAGACCCC CAGGAAACAG AGGCTCGCAT TGTGGGGGGC
    CGCTTTGATG CCTTCCAGCT GTATCCCAAC ACATCCCAGA GTGCCACCCT CTTCTTGCGA
    AAGGTCCTCT CAGTACATCC CTGGAGCCGG CCCTCCGTGC AGGACTGCCT GGCTCACCCG
    TGGCTGCAGG ATGCCTACCT GATGAAACTG CGCCGCCAGA CACTCACCTT CACCACCAAC
    CGGCTCAAGG AGTTCCTGGG TGAGCAGCGG CGCCGCAGGG CCGAGGCCGC CACCCGCCAC
    AAGGTGCTGC TCCGCTCTTA CCCAGGCAGC CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202905.1
    CDS339..7634
    Translation

    Target ORF information:

    RefSeq Version XM_005202848.3
    Organism Bos taurus(cattle)
    Definition Bos taurus SPEG complex locus (SPEG), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202848.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAAGCTCA GTCCTGGCCA GAATCGCCGT TCCTCCGACA CTGGCTCCAA GGCGCCCCCT 
    ACTTTCAAGG TTTCACTTAT GGACCAGTCA GTAAGGGAAG GCCAAGACGT CACCATGAGC
    ATCCGTGTGC AGGGCGAACC CAAGCCGGTG GTCTCCTGGC TGAGAAACCG TCAGCCTGTG
    CGCCCAGACC AGCGGCGCTT TGCTGAGGAG GCAGAGGATG GGCTCTGCCG GCTTCGGATC
    CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC TACACCTGCA AGGCGGTTAA CGAGTATGGC
    GCTCGACAGT GTGAGGCGCG CCTGGAGGTC AGAGCACACC CTGAAAGCCG GTCCCTGGCC
    GTGCTGGCCC CTCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC
    CTGGTGGCGG GTCCCACCGA CGTGGAGGTA GACTGGCTGT GCCGAGGCCG CCTGCTGCAG
    CCTGCACTAC TCAAATGCAA GATGCATTTC GATGGCCGCA AATGCAAGCT GCTGCTCACC
    TCTGTGCATG AGGACGACAG CGGCGTCTAC ACCTGCAAGC TCAGCACGGC CAAAGATGAA
    CTGACCTGCA GTGCCCGGCT GACCGTGCGA CCCTCGCTGG CACCCCTGTT CACGCGGCTG
    CTGGAGGACG TGGAAGTGCT GGAGGGCCGC GCTGCCCGCT TCGACTGCAA GATCAGCGGG
    AGCCCGCCCC CCTCCGTTAC CTGGACTCAC TTTGGCCACC CCGTGGAGGA GAGCGAGAAC
    GTGCGGCTGC GGCAGGACGG GGGCCTGCAT TCGCTGCACA TCGCACATGT GGGCAGTGAG
    GATGAGGGGC TGTACGCGGT CAGTGCTGCC AACACCCACG GCCAGGCCCA CTGCTCAGCC
    CAGCTCTACG TGGAGGAGCC TCGGACGGCT GCCACCGGCC CTAGCTCCAA GCTGGAGAAG
    ATGCCCTCCA TCCCCGAGGA GCCCGAGCAG GGTGAGCTGG AGCGGCTGTC CATGCCCGAC
    TTCCTGCGGC CGCTGCAGGA TCTGGACGTG GGACTGGCCA AGGAGGCCAT GCTGGAGTGC
    CAGGTGACCG GCCTGCCCTA CCCCACCATC AGCTGGTTCC ACAACGGCCA CCGCATCCAG
    AGCAGCGACG ACCGACGCAT GACGCAGTAC AGGGATGTCC ATCGCTTGGT GTTCCCTGCC
    GTGGGACCTC AGCATGCTGG TGTCTACAAG AGCGTCATTG CTAACAAGCT GGGCAAAGCC
    GCCTGCTATG CTCATCTCTA CGTCACAGAT GTGGTTCCAG GCCCCCCAGA CGGTGCCCCT
    CAGGTAGTGG CTGTGACCGG GAGGATGATC ACGCTTGCAT GGAACCCCCC GAGGAGTCTG
    GACATGGCTA TCGACCCAGA CGCCCTGACC TACACCGTGC AGCACCAGGT GCTGGGCTCA
    GACCAGTGGA CGGCGCTGGT CACGGGCCTG CGGCAGCCCG GGTGGGCAGC CACCGGATTG
    CGAAAGGGGG TCCAGCACGT CTTCCGGGTT CTCAGCACCA CCATCAAGAG CAGCAGCAAG
    CCCTCGCCCC CGTCCGAGCC CACGCAGCTG CTGGAGCGTG GCCCGCCCCT GGAGGAGGCG
    CCCGCTGTGC TGGACAAGCC AGACGTCGTG TATGTGGTGG AGGGCCAGCC CACCAGTGTC
    ACCGTCACCT TCAACCACGT GGAGGCCCAG GTTGTCTGGA GGAGCTGCCG AGGAGCCCTC
    CTAGAGGCCC GGCCAGGTGT GTTTGAGCTG AGCCAGCCAG ATGACGACCA GTACTCTCTT
    AGGATCTGCC GGGTGAGCCG CCGGGACCTG GGAGCCCTCA CTTGCACTGC CCGCAACCGC
    CACGGCACAC AGGCCTGCTC AGTCACCCTG GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC
    ATCATGGAGG ATGTGGAGGT AGGGGCTGGA GAGACTGCTC GCTTCGCCGT GGTGGTGGAC
    GGGAAGCCCT TGCCGGACAT CATGTGGTAC AAGGATGAGG CGCTGCTGAC CGAGAGCAAC
    CATGTGAGCT TCGTGTACGA GGAGAACGAG TGCTCCCTGG TGGTGCTCAG CGCGGGGGCC
    CAGGATGGAG GGGTCTACAC CTGCACGGCC CGTAACCTGG CTGGCGAAGT CTCCTGCAAA
    GCAGAGCTGG CTGTGCACTC AGCTCAGACA GCTATGGAGG TGGAAGGGAT CGGGGAAGAC
    GAGGAGCAGC GAGGAAGGAG GCTCAGCGAC TTCTATGATA TTCAGCAGGA GATCGGCAGG
    GGTGCCTTCT CCTACCTGCG CCGTGTAGTG GAGAGGAGCT CCGGACTGGA GTTTGCAGCC
    AAGTTCATCC CCAGCCAGGC AAAGCCAAAG GCATCGGCAT GGCGGGAGGC ACGGCTGCTG
    GCCCGGCTCC AGCATGACTG CATCCTCTAC TTCCATGAGG CCTTCGAGAG GCGCCGGGGA
    CTGGTCATCG TCACCGAGCT CTGCACAGAA GAGCTTCTGG AGCGAATGGC CAGGAAACCC
    ACCGTGTGTG AGTCTGAGAT CCGGGCCTAC ATGCGGCAGG TACTAGAAGG AATATGCTAC
    TTGCACGAGA ACCACGTGCT GCACCTGGAT GTCAAGCCCG AGAACCTGCT GGTGTGGGAT
    GGTGTTGAGG GCGAGGAGCA GGTGAGGATC TGTGACTTTG GGAACGCTCA AGAGCTGACA
    CCGGGAGAGC CCCAGTATTG CCAATACGGC ACACCTGAGT TCGTGGCGCC CGAGATTGTC
    AACCAGACCC CTGTGTCTGG AGTCACTGAC ATCTGGCCCG TGGGTGTCGT CGCCTTCCTC
    TGCCTGACCG GAATCTCCCC GTTCGTTGGG GAAAATGATC GGACGACATT GATGAACATC
    CGAAACTACA ACGTGGCCTT CGAGGAGACC ACGTTCCTGA GCCTGAGCAG GGAGGCCCGG
    GGCTTCCTCA TCAAAGTGCT GGTGCGGGAC CGGCTGAGGC CTACTGCAGA GGAGACCCTG
    GAACATCCTT GGTTTAAAGC ACAGGCAAAG GGTGCAGTGG TGAGCACGGA TCACCTAAAG
    CTATTCCTCT CCCGGCGGAG GTGGCAGCGC TCCCAGATCA GCTACAAGTG CCACCTGGTG
    CTGCGCCCCA TCCCGGAGCT GCTGCGAGCA CCCCCGGAGC GGGTGTGGGT GGCCGTGCCC
    CGAAGACCAC CCCCTAGTGG AGGGCTGTCG TCATCATCCG ACTCTGAAGA GGAAGAGCTG
    GAGGAGCTGC CCTCTGTGCC CCGACCTCTG CAGCCTGAGT TTTCGGGCTC CCGGGTGTCC
    CTCACTGACA TTCCCACTGA GGATGAGGCC CTCGGGGCCC TGGAGGCTGG GGCGGCCACC
    CCCATGGACT GGCAAGAACA AGGAAAGGCC CCCTCTCAGG ACCAGGAGGC CCCCAGCCCT
    CAGGCACTCC CCTCCCCAGG CCAGGAGTCC CCGTCCGGGC CCAGCCCCCG GCGGGCAGAG
    CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC CTGCCCCGGG CCGGGCCGAG GGAACCGGGC
    CGGGGCCTGC ACAAGGCAGC GTCCGTCGAG CTGCCGCAGC GCCGAAGCCC CAGCCCGGGG
    GCCACCCGCC TGACCCGAGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC
    CTGCGCCAGC GGCTGCTGAG GGGTGGCCCC GATGATGGCA AGGTCAGCGG CCTCAGGGGC
    CCCCTGCTGG AGAGCCTGGG GGGCCGGGCT CGTGACCCCC GGCTGGCGCG GGCAGCCTCC
    AGCGAGGCAG CACCCCATCA CCAGCACCCA CCTGAGGCTC GGGGCCTGCA GAAGAGCAGT
    AGCTTCTCGC AGGGTGAGGC AGAGCCCCGG GGCCGGCACC GCCGAGCAGG GGCACCCCTT
    GAGATCCCCG TGGCCCGGCT GGGGGCCCGC AAGCTGCAGG AGTCTCCCTC CTTGTCCGCC
    CTCAGCGAGG CCCAACCACC TAGCCCCGTG CGGCCCAGTG CAGCCAAGCC CAGCACCCCT
    AAGTCTTCAG AACCTCTTGT GGGCACCCCC AGTGAGGCTG CACAGCCTCC AGCACCCCAG
    CCTGCCCGAG AGAAGGCCCC AGAATCTACG CCAGAACCAG CTCGGGGCCC CAAGCCCACA
    CAGCCCCCCA CAGCTCTGCA AACCCTAGCG CCTCCTCTCA CTCCGTATGC GCAGATCATG
    CAGTCGCTCC AGCTGCCAGG CCAGGCGCAA GGCCCCCCAC AGGGCGCAGT CGTATCTCCT
    ACATCTCGGT CTCCAGCATC TCGGTTGGAA CCCAAGCCCC ACCCGGCTGT GTTCTCCAGG
    GTGGCCTCCC CACCTCCAGG AGCCTCCGAG AAGCGCCTGC TGCCGGGAGC CGGAGCAACC
    CCAGGGCCAG CCGAAAAAGC CCGGGTCCCG GCGGTCCCCC GCAGGCCAGG GAGCAGTCTC
    AGCAGCAGCA TCGAGAACCT GGAGTCGGAG GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC
    CGGGAGTCGC CCCTGTCGCG GGGGCTGCGG CTGCTGAGCC GCTCGCGGTC GGAGGAGCGC
    GGCCCCTTCC GCGGGGCTGA GGAGGAGGAC GGCATTTACC GGCCCAGCCC GGCGGGCACG
    CCGCTGGAGC TGGTGCGACG GCCCGAGCGC TCGCGCTCCG TGCAGGACCT CAGGGCGGTC
    GGGGAGCCAG GCCTCGTGCG CCGCCTCTCG CTGTCGCTGT CCCAGCGTCT GCGGCGGACC
    CCGCCCGCGC AGCGCCACCC GGCCTGGGAG CCCCGCGGCG GGGACGGCGA GAGCTCGGAG
    GGCGGCAGCT CGGCGCGGGG CTCCCCGGTG CTGACGGTGC GCCGGAGGCT CAGCTCCACG
    CTGGAGCGGC TGTCGAGCCG GTTGCAGCGC AGCGGCAGCA GCGAGGACTC GGGGGGCGCG
    TCGGGCCGCA GCACGCCGCT GTTCGGCCGG CTGCGCAGGG CCACGTCCGA GAGCGAGAGT
    CTGCGGCGCC TGGACCTCGC GCAAAATCAG CTGGCGGCCC AAGCCGGCGC CGCCACCCCT
    TCCGCGGAGT CCCTGGGCTC CGAGGCCAGC GCTACGTCGG GCTCCTCTGC TCCTGCAGAA
    AGTCGAAGCC GCCTGCGCTG GGGCCTCTCT CGGCTGCGGA AGGACAAGAG GTTGTCACAG
    CCTAACCTCT CTGGCAGTGT CCAGGAGGAT TTGGGTCACC AGTATGTGCG CAGTGAGTCA
    GACTTTCCCC CAGTCTTCCA CATCAAACTC AAGGACCAGG TGCTGCTGGA GGGAGAGGCA
    GCCACCCTGC TCTGCTTGCC GGCAGCCTGC CCTGCACCCC ACATCTCCTG GATGAAAGAC
    AAGCAGTCCC TGCGGTCAGA GCCCTCGGTG ATCATCGTGT CCTGCAAAGA TGGTCGTCAG
    CTACTGAGCA TCCCCCGGGC AGGCAAGCGG CATGCGGGGC TCTATGAGTG CTCCGCCACC
    AACGTCCTTG GCAGCATCAC CAGCTCCTGT ACCGTGGCTG TGGCTCGTAC CCCAGGAAAG
    CTAGCTCCTC CTGAGGTGCC CCAGACCTAC CAGGACACAG CACTGGTGCT CTGGAAGCCG
    GGAGACAGCA AGGCGCCTTG CACATACACG CTGGAGCGGC GGGTGGATGG GGAGTCTTCC
    TGGCACCCTG TGAGCTCGGG CATCCCCGAC TGCTACTACA ACGTGACCCA CCTACCAGTC
    GGCATGGCTG TGAGGTTCCG TGTGGCCTGT GCCAACCGTG CTGGGCAAGG GCCTTTCAGC
    AACCCTTCTG AGAAGGTCCT CGTCAGGGGC GTGCAAGATT CCTCAGCTCT GCCATCTGCT
    GCTCACAGAG ATGCCCCTGT TACCTCAGGG CCAGCCAGGG CCCCGCCTCC TGACGCTCCT
    ACCTCACTGG CCCCTCCCCT GAGCCCCTCC CCAGCTCCTG CTCCCCCAGG CCCCCAGGCA
    GCCCCTCTCG GCCCTTCATC TTCCCCAGCA CCCCCCAGCC AGGCCTTGTC CTCCCTCAAG
    GCTGTGGGTC CACCACCCCA AACCCCCCCA CGAAAGCACC GGGGCCTGCA GGCTGCCCAG
    CAAGTAGAAC CTACCCCACC CAGTGCCCAG GACTCCCCAA GTGAGCCCAA GTCTTCCGTC
    CCTGACACTG GGACCCCCAT CCCAGCCTCT GCCCCTCAAG TGGTGAAAAC AGCCTCTTCC
    TCAATGCCCC TGTATATGGT GACCTCCTTT GTGTCTGCAC CACCAGCCCC TGAGCCCCCA
    GCCCCCGAGC CCCCTCCTGA GCCCACCAAA GTGACTGTGC GGAGCCTCAG CCCAGCCAAG
    GAGGTGGTTG GCTCCCCTGG GGGTGGTCCC CCCAGGGCTC CTAAGCCAGA GGGCACCACT
    CTTCGACAGG GCCCTCCTCA GAAACCATAC ACCTTCCTGG AGGAGAAGGC CAGGGGTCGC
    TTTGGTGTGG TGCGTGCCTG CCGGGAGAAT GCCACCGGGC GAACATTCGT GGCCAAGATT
    GTGCCATACG CGGCCGAGGG CAAGCGGCAG GTCCTGCAGG AGTACGAGGT GCTGAGGACC
    CTGCACCACG AGCGGCTTAT GGCCCTGCAC GAAGCCTACA TCACCCCGCG CTACCTCGTG
    CTCATCGCCG AGAGCTGCGG CAACCGGGAG CTTCTCTGTG GGCTCAGCGA CAGATTCCGG
    TATTCAGAGG ATGATGTGGC CACCTATGTG GTGCAGCTGC TACAAGGCCT GGATTACCTC
    CATGGCCGCC ACGTGCTGCA CCTGGACATC AAGCCAGACA ACCTGCTGCT GGCCCCTGAC
    AACGCCCTCA AGATCGTGGA CTTTGGCAGT GCCCAGCCCT ACAACCCCCA GGCTCTGCGG
    CCCCTGGGTC ACCGCACGGG CACACTGGAG TTCATGGCTC CTGAGATGGT AAAGGGAGAC
    CCCATCGGCT CTGCCACGGA CATCTGGGGA GCGGGTGTGC TCACTTACAT CATGCTCAGT
    GGACGCTCCC CATTCTATGA GCCAGACCCC CAGGAAACAG AGGCTCGCAT TGTGGGGGGC
    CGCTTTGATG CCTTCCAGCT GTATCCCAAC ACATCCCAGA GTGCCACCCT CTTCTTGCGA
    AAGGTCCTCT CAGTACATCC CTGGAGCCGG CCCTCCGTGC AGGACTGCCT GGCTCACCCG
    TGGCTGCAGG ATGCCTACCT GATGAAACTG CGCCGCCAGA CACTCACCTT CACCACCAAC
    CGGCTCAAGG AGTTCCTGGG TGAGCAGCGG CGCCGCAGGG CCGAGGCCGC CACCCGCCAC
    AAGGTGCTGC TCCGCTCTTA CCCAGGCAGC CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16713
    Clone ID Related Accession (Same CDS sequence) XM_015459169.1 , XM_005202848.3 , XM_005202849.3
    Accession Version XM_005202849.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product striated muscle preferentially expressed protein kinase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103849.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202849.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAAGCTCA GTCCTGGCCA GAATCGCCGT TCCTCCGACA CTGGCTCCAA GGCGCCCCCT 
    ACTTTCAAGG TTTCACTTAT GGACCAGTCA GTAAGGGAAG GCCAAGACGT CACCATGAGC
    ATCCGTGTGC AGGGCGAACC CAAGCCGGTG GTCTCCTGGC TGAGAAACCG TCAGCCTGTG
    CGCCCAGACC AGCGGCGCTT TGCTGAGGAG GCAGAGGATG GGCTCTGCCG GCTTCGGATC
    CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC TACACCTGCA AGGCGGTTAA CGAGTATGGC
    GCTCGACAGT GTGAGGCGCG CCTGGAGGTC AGAGCACACC CTGAAAGCCG GTCCCTGGCC
    GTGCTGGCCC CTCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC
    CTGGTGGCGG GTCCCACCGA CGTGGAGGTA GACTGGCTGT GCCGAGGCCG CCTGCTGCAG
    CCTGCACTAC TCAAATGCAA GATGCATTTC GATGGCCGCA AATGCAAGCT GCTGCTCACC
    TCTGTGCATG AGGACGACAG CGGCGTCTAC ACCTGCAAGC TCAGCACGGC CAAAGATGAA
    CTGACCTGCA GTGCCCGGCT GACCGTGCGA CCCTCGCTGG CACCCCTGTT CACGCGGCTG
    CTGGAGGACG TGGAAGTGCT GGAGGGCCGC GCTGCCCGCT TCGACTGCAA GATCAGCGGG
    AGCCCGCCCC CCTCCGTTAC CTGGACTCAC TTTGGCCACC CCGTGGAGGA GAGCGAGAAC
    GTGCGGCTGC GGCAGGACGG GGGCCTGCAT TCGCTGCACA TCGCACATGT GGGCAGTGAG
    GATGAGGGGC TGTACGCGGT CAGTGCTGCC AACACCCACG GCCAGGCCCA CTGCTCAGCC
    CAGCTCTACG TGGAGGAGCC TCGGACGGCT GCCACCGGCC CTAGCTCCAA GCTGGAGAAG
    ATGCCCTCCA TCCCCGAGGA GCCCGAGCAG GGTGAGCTGG AGCGGCTGTC CATGCCCGAC
    TTCCTGCGGC CGCTGCAGGA TCTGGACGTG GGACTGGCCA AGGAGGCCAT GCTGGAGTGC
    CAGGTGACCG GCCTGCCCTA CCCCACCATC AGCTGGTTCC ACAACGGCCA CCGCATCCAG
    AGCAGCGACG ACCGACGCAT GACGCAGTAC AGGGATGTCC ATCGCTTGGT GTTCCCTGCC
    GTGGGACCTC AGCATGCTGG TGTCTACAAG AGCGTCATTG CTAACAAGCT GGGCAAAGCC
    GCCTGCTATG CTCATCTCTA CGTCACAGAT GTGGTTCCAG GCCCCCCAGA CGGTGCCCCT
    CAGGTAGTGG CTGTGACCGG GAGGATGATC ACGCTTGCAT GGAACCCCCC GAGGAGTCTG
    GACATGGCTA TCGACCCAGA CGCCCTGACC TACACCGTGC AGCACCAGGT GCTGGGCTCA
    GACCAGTGGA CGGCGCTGGT CACGGGCCTG CGGCAGCCCG GGTGGGCAGC CACCGGATTG
    CGAAAGGGGG TCCAGCACGT CTTCCGGGTT CTCAGCACCA CCATCAAGAG CAGCAGCAAG
    CCCTCGCCCC CGTCCGAGCC CACGCAGCTG CTGGAGCGTG GCCCGCCCCT GGAGGAGGCG
    CCCGCTGTGC TGGACAAGCC AGACGTCGTG TATGTGGTGG AGGGCCAGCC CACCAGTGTC
    ACCGTCACCT TCAACCACGT GGAGGCCCAG GTTGTCTGGA GGAGCTGCCG AGGAGCCCTC
    CTAGAGGCCC GGCCAGGTGT GTTTGAGCTG AGCCAGCCAG ATGACGACCA GTACTCTCTT
    AGGATCTGCC GGGTGAGCCG CCGGGACCTG GGAGCCCTCA CTTGCACTGC CCGCAACCGC
    CACGGCACAC AGGCCTGCTC AGTCACCCTG GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC
    ATCATGGAGG ATGTGGAGGT AGGGGCTGGA GAGACTGCTC GCTTCGCCGT GGTGGTGGAC
    GGGAAGCCCT TGCCGGACAT CATGTGGTAC AAGGATGAGG CGCTGCTGAC CGAGAGCAAC
    CATGTGAGCT TCGTGTACGA GGAGAACGAG TGCTCCCTGG TGGTGCTCAG CGCGGGGGCC
    CAGGATGGAG GGGTCTACAC CTGCACGGCC CGTAACCTGG CTGGCGAAGT CTCCTGCAAA
    GCAGAGCTGG CTGTGCACTC AGCTCAGACA GCTATGGAGG TGGAAGGGAT CGGGGAAGAC
    GAGGAGCAGC GAGGAAGGAG GCTCAGCGAC TTCTATGATA TTCAGCAGGA GATCGGCAGG
    GGTGCCTTCT CCTACCTGCG CCGTGTAGTG GAGAGGAGCT CCGGACTGGA GTTTGCAGCC
    AAGTTCATCC CCAGCCAGGC AAAGCCAAAG GCATCGGCAT GGCGGGAGGC ACGGCTGCTG
    GCCCGGCTCC AGCATGACTG CATCCTCTAC TTCCATGAGG CCTTCGAGAG GCGCCGGGGA
    CTGGTCATCG TCACCGAGCT CTGCACAGAA GAGCTTCTGG AGCGAATGGC CAGGAAACCC
    ACCGTGTGTG AGTCTGAGAT CCGGGCCTAC ATGCGGCAGG TACTAGAAGG AATATGCTAC
    TTGCACGAGA ACCACGTGCT GCACCTGGAT GTCAAGCCCG AGAACCTGCT GGTGTGGGAT
    GGTGTTGAGG GCGAGGAGCA GGTGAGGATC TGTGACTTTG GGAACGCTCA AGAGCTGACA
    CCGGGAGAGC CCCAGTATTG CCAATACGGC ACACCTGAGT TCGTGGCGCC CGAGATTGTC
    AACCAGACCC CTGTGTCTGG AGTCACTGAC ATCTGGCCCG TGGGTGTCGT CGCCTTCCTC
    TGCCTGACCG GAATCTCCCC GTTCGTTGGG GAAAATGATC GGACGACATT GATGAACATC
    CGAAACTACA ACGTGGCCTT CGAGGAGACC ACGTTCCTGA GCCTGAGCAG GGAGGCCCGG
    GGCTTCCTCA TCAAAGTGCT GGTGCGGGAC CGGCTGAGGC CTACTGCAGA GGAGACCCTG
    GAACATCCTT GGTTTAAAGC ACAGGCAAAG GGTGCAGTGG TGAGCACGGA TCACCTAAAG
    CTATTCCTCT CCCGGCGGAG GTGGCAGCGC TCCCAGATCA GCTACAAGTG CCACCTGGTG
    CTGCGCCCCA TCCCGGAGCT GCTGCGAGCA CCCCCGGAGC GGGTGTGGGT GGCCGTGCCC
    CGAAGACCAC CCCCTAGTGG AGGGCTGTCG TCATCATCCG ACTCTGAAGA GGAAGAGCTG
    GAGGAGCTGC CCTCTGTGCC CCGACCTCTG CAGCCTGAGT TTTCGGGCTC CCGGGTGTCC
    CTCACTGACA TTCCCACTGA GGATGAGGCC CTCGGGGCCC TGGAGGCTGG GGCGGCCACC
    CCCATGGACT GGCAAGAACA AGGAAAGGCC CCCTCTCAGG ACCAGGAGGC CCCCAGCCCT
    CAGGCACTCC CCTCCCCAGG CCAGGAGTCC CCGTCCGGGC CCAGCCCCCG GCGGGCAGAG
    CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC CTGCCCCGGG CCGGGCCGAG GGAACCGGGC
    CGGGGCCTGC ACAAGGCAGC GTCCGTCGAG CTGCCGCAGC GCCGAAGCCC CAGCCCGGGG
    GCCACCCGCC TGACCCGAGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC
    CTGCGCCAGC GGCTGCTGAG GGGTGGCCCC GATGATGGCA AGGTCAGCGG CCTCAGGGGC
    CCCCTGCTGG AGAGCCTGGG GGGCCGGGCT CGTGACCCCC GGCTGGCGCG GGCAGCCTCC
    AGCGAGGCAG CACCCCATCA CCAGCACCCA CCTGAGGCTC GGGGCCTGCA GAAGAGCAGT
    AGCTTCTCGC AGGGTGAGGC AGAGCCCCGG GGCCGGCACC GCCGAGCAGG GGCACCCCTT
    GAGATCCCCG TGGCCCGGCT GGGGGCCCGC AAGCTGCAGG AGTCTCCCTC CTTGTCCGCC
    CTCAGCGAGG CCCAACCACC TAGCCCCGTG CGGCCCAGTG CAGCCAAGCC CAGCACCCCT
    AAGTCTTCAG AACCTCTTGT GGGCACCCCC AGTGAGGCTG CACAGCCTCC AGCACCCCAG
    CCTGCCCGAG AGAAGGCCCC AGAATCTACG CCAGAACCAG CTCGGGGCCC CAAGCCCACA
    CAGCCCCCCA CAGCTCTGCA AACCCTAGCG CCTCCTCTCA CTCCGTATGC GCAGATCATG
    CAGTCGCTCC AGCTGCCAGG CCAGGCGCAA GGCCCCCCAC AGGGCGCAGT CGTATCTCCT
    ACATCTCGGT CTCCAGCATC TCGGTTGGAA CCCAAGCCCC ACCCGGCTGT GTTCTCCAGG
    GTGGCCTCCC CACCTCCAGG AGCCTCCGAG AAGCGCCTGC TGCCGGGAGC CGGAGCAACC
    CCAGGGCCAG CCGAAAAAGC CCGGGTCCCG GCGGTCCCCC GCAGGCCAGG GAGCAGTCTC
    AGCAGCAGCA TCGAGAACCT GGAGTCGGAG GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC
    CGGGAGTCGC CCCTGTCGCG GGGGCTGCGG CTGCTGAGCC GCTCGCGGTC GGAGGAGCGC
    GGCCCCTTCC GCGGGGCTGA GGAGGAGGAC GGCATTTACC GGCCCAGCCC GGCGGGCACG
    CCGCTGGAGC TGGTGCGACG GCCCGAGCGC TCGCGCTCCG TGCAGGACCT CAGGGCGGTC
    GGGGAGCCAG GCCTCGTGCG CCGCCTCTCG CTGTCGCTGT CCCAGCGTCT GCGGCGGACC
    CCGCCCGCGC AGCGCCACCC GGCCTGGGAG CCCCGCGGCG GGGACGGCGA GAGCTCGGAG
    GGCGGCAGCT CGGCGCGGGG CTCCCCGGTG CTGACGGTGC GCCGGAGGCT CAGCTCCACG
    CTGGAGCGGC TGTCGAGCCG GTTGCAGCGC AGCGGCAGCA GCGAGGACTC GGGGGGCGCG
    TCGGGCCGCA GCACGCCGCT GTTCGGCCGG CTGCGCAGGG CCACGTCCGA GAGCGAGAGT
    CTGCGGCGCC TGGACCTCGC GCAAAATCAG CTGGCGGCCC AAGCCGGCGC CGCCACCCCT
    TCCGCGGAGT CCCTGGGCTC CGAGGCCAGC GCTACGTCGG GCTCCTCTGC TCCTGCAGAA
    AGTCGAAGCC GCCTGCGCTG GGGCCTCTCT CGGCTGCGGA AGGACAAGAG GTTGTCACAG
    CCTAACCTCT CTGGCAGTGT CCAGGAGGAT TTGGGTCACC AGTATGTGCG CAGTGAGTCA
    GACTTTCCCC CAGTCTTCCA CATCAAACTC AAGGACCAGG TGCTGCTGGA GGGAGAGGCA
    GCCACCCTGC TCTGCTTGCC GGCAGCCTGC CCTGCACCCC ACATCTCCTG GATGAAAGAC
    AAGCAGTCCC TGCGGTCAGA GCCCTCGGTG ATCATCGTGT CCTGCAAAGA TGGTCGTCAG
    CTACTGAGCA TCCCCCGGGC AGGCAAGCGG CATGCGGGGC TCTATGAGTG CTCCGCCACC
    AACGTCCTTG GCAGCATCAC CAGCTCCTGT ACCGTGGCTG TGGCTCGTAC CCCAGGAAAG
    CTAGCTCCTC CTGAGGTGCC CCAGACCTAC CAGGACACAG CACTGGTGCT CTGGAAGCCG
    GGAGACAGCA AGGCGCCTTG CACATACACG CTGGAGCGGC GGGTGGATGG GGAGTCTTCC
    TGGCACCCTG TGAGCTCGGG CATCCCCGAC TGCTACTACA ACGTGACCCA CCTACCAGTC
    GGCATGGCTG TGAGGTTCCG TGTGGCCTGT GCCAACCGTG CTGGGCAAGG GCCTTTCAGC
    AACCCTTCTG AGAAGGTCCT CGTCAGGGGC GTGCAAGATT CCTCAGCTCT GCCATCTGCT
    GCTCACAGAG ATGCCCCTGT TACCTCAGGG CCAGCCAGGG CCCCGCCTCC TGACGCTCCT
    ACCTCACTGG CCCCTCCCCT GAGCCCCTCC CCAGCTCCTG CTCCCCCAGG CCCCCAGGCA
    GCCCCTCTCG GCCCTTCATC TTCCCCAGCA CCCCCCAGCC AGGCCTTGTC CTCCCTCAAG
    GCTGTGGGTC CACCACCCCA AACCCCCCCA CGAAAGCACC GGGGCCTGCA GGCTGCCCAG
    CAAGTAGAAC CTACCCCACC CAGTGCCCAG GACTCCCCAA GTGAGCCCAA GTCTTCCGTC
    CCTGACACTG GGACCCCCAT CCCAGCCTCT GCCCCTCAAG TGGTGAAAAC AGCCTCTTCC
    TCAATGCCCC TGTATATGGT GACCTCCTTT GTGTCTGCAC CACCAGCCCC TGAGCCCCCA
    GCCCCCGAGC CCCCTCCTGA GCCCACCAAA GTGACTGTGC GGAGCCTCAG CCCAGCCAAG
    GAGGTGGTTG GCTCCCCTGG GGGTGGTCCC CCCAGGGCTC CTAAGCCAGA GGGCACCACT
    CTTCGACAGG GCCCTCCTCA GAAACCATAC ACCTTCCTGG AGGAGAAGGC CAGGGGTCGC
    TTTGGTGTGG TGCGTGCCTG CCGGGAGAAT GCCACCGGGC GAACATTCGT GGCCAAGATT
    GTGCCATACG CGGCCGAGGG CAAGCGGCAG GTCCTGCAGG AGTACGAGGT GCTGAGGACC
    CTGCACCACG AGCGGCTTAT GGCCCTGCAC GAAGCCTACA TCACCCCGCG CTACCTCGTG
    CTCATCGCCG AGAGCTGCGG CAACCGGGAG CTTCTCTGTG GGCTCAGCGA CAGATTCCGG
    TATTCAGAGG ATGATGTGGC CACCTATGTG GTGCAGCTGC TACAAGGCCT GGATTACCTC
    CATGGCCGCC ACGTGCTGCA CCTGGACATC AAGCCAGACA ACCTGCTGCT GGCCCCTGAC
    AACGCCCTCA AGATCGTGGA CTTTGGCAGT GCCCAGCCCT ACAACCCCCA GGCTCTGCGG
    CCCCTGGGTC ACCGCACGGG CACACTGGAG TTCATGGCTC CTGAGATGGT AAAGGGAGAC
    CCCATCGGCT CTGCCACGGA CATCTGGGGA GCGGGTGTGC TCACTTACAT CATGCTCAGT
    GGACGCTCCC CATTCTATGA GCCAGACCCC CAGGAAACAG AGGCTCGCAT TGTGGGGGGC
    CGCTTTGATG CCTTCCAGCT GTATCCCAAC ACATCCCAGA GTGCCACCCT CTTCTTGCGA
    AAGGTCCTCT CAGTACATCC CTGGAGCCGG CCCTCCGTGC AGGACTGCCT GGCTCACCCG
    TGGCTGCAGG ATGCCTACCT GATGAAACTG CGCCGCCAGA CACTCACCTT CACCACCAAC
    CGGCTCAAGG AGTTCCTGGG TGAGCAGCGG CGCCGCAGGG CCGAGGCCGC CACCCGCCAC
    AAGGTGCTGC TCCGCTCTTA CCCAGGCAGC CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202906.1
    CDS441..7736
    Translation

    Target ORF information:

    RefSeq Version XM_005202849.3
    Organism Bos taurus(cattle)
    Definition Bos taurus SPEG complex locus (SPEG), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202849.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAAGCTCA GTCCTGGCCA GAATCGCCGT TCCTCCGACA CTGGCTCCAA GGCGCCCCCT 
    ACTTTCAAGG TTTCACTTAT GGACCAGTCA GTAAGGGAAG GCCAAGACGT CACCATGAGC
    ATCCGTGTGC AGGGCGAACC CAAGCCGGTG GTCTCCTGGC TGAGAAACCG TCAGCCTGTG
    CGCCCAGACC AGCGGCGCTT TGCTGAGGAG GCAGAGGATG GGCTCTGCCG GCTTCGGATC
    CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC TACACCTGCA AGGCGGTTAA CGAGTATGGC
    GCTCGACAGT GTGAGGCGCG CCTGGAGGTC AGAGCACACC CTGAAAGCCG GTCCCTGGCC
    GTGCTGGCCC CTCTGCAGGA CGTGGACGTG GGGGCCGGGG AGATGGCGCT GTTTGAGTGC
    CTGGTGGCGG GTCCCACCGA CGTGGAGGTA GACTGGCTGT GCCGAGGCCG CCTGCTGCAG
    CCTGCACTAC TCAAATGCAA GATGCATTTC GATGGCCGCA AATGCAAGCT GCTGCTCACC
    TCTGTGCATG AGGACGACAG CGGCGTCTAC ACCTGCAAGC TCAGCACGGC CAAAGATGAA
    CTGACCTGCA GTGCCCGGCT GACCGTGCGA CCCTCGCTGG CACCCCTGTT CACGCGGCTG
    CTGGAGGACG TGGAAGTGCT GGAGGGCCGC GCTGCCCGCT TCGACTGCAA GATCAGCGGG
    AGCCCGCCCC CCTCCGTTAC CTGGACTCAC TTTGGCCACC CCGTGGAGGA GAGCGAGAAC
    GTGCGGCTGC GGCAGGACGG GGGCCTGCAT TCGCTGCACA TCGCACATGT GGGCAGTGAG
    GATGAGGGGC TGTACGCGGT CAGTGCTGCC AACACCCACG GCCAGGCCCA CTGCTCAGCC
    CAGCTCTACG TGGAGGAGCC TCGGACGGCT GCCACCGGCC CTAGCTCCAA GCTGGAGAAG
    ATGCCCTCCA TCCCCGAGGA GCCCGAGCAG GGTGAGCTGG AGCGGCTGTC CATGCCCGAC
    TTCCTGCGGC CGCTGCAGGA TCTGGACGTG GGACTGGCCA AGGAGGCCAT GCTGGAGTGC
    CAGGTGACCG GCCTGCCCTA CCCCACCATC AGCTGGTTCC ACAACGGCCA CCGCATCCAG
    AGCAGCGACG ACCGACGCAT GACGCAGTAC AGGGATGTCC ATCGCTTGGT GTTCCCTGCC
    GTGGGACCTC AGCATGCTGG TGTCTACAAG AGCGTCATTG CTAACAAGCT GGGCAAAGCC
    GCCTGCTATG CTCATCTCTA CGTCACAGAT GTGGTTCCAG GCCCCCCAGA CGGTGCCCCT
    CAGGTAGTGG CTGTGACCGG GAGGATGATC ACGCTTGCAT GGAACCCCCC GAGGAGTCTG
    GACATGGCTA TCGACCCAGA CGCCCTGACC TACACCGTGC AGCACCAGGT GCTGGGCTCA
    GACCAGTGGA CGGCGCTGGT CACGGGCCTG CGGCAGCCCG GGTGGGCAGC CACCGGATTG
    CGAAAGGGGG TCCAGCACGT CTTCCGGGTT CTCAGCACCA CCATCAAGAG CAGCAGCAAG
    CCCTCGCCCC CGTCCGAGCC CACGCAGCTG CTGGAGCGTG GCCCGCCCCT GGAGGAGGCG
    CCCGCTGTGC TGGACAAGCC AGACGTCGTG TATGTGGTGG AGGGCCAGCC CACCAGTGTC
    ACCGTCACCT TCAACCACGT GGAGGCCCAG GTTGTCTGGA GGAGCTGCCG AGGAGCCCTC
    CTAGAGGCCC GGCCAGGTGT GTTTGAGCTG AGCCAGCCAG ATGACGACCA GTACTCTCTT
    AGGATCTGCC GGGTGAGCCG CCGGGACCTG GGAGCCCTCA CTTGCACTGC CCGCAACCGC
    CACGGCACAC AGGCCTGCTC AGTCACCCTG GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC
    ATCATGGAGG ATGTGGAGGT AGGGGCTGGA GAGACTGCTC GCTTCGCCGT GGTGGTGGAC
    GGGAAGCCCT TGCCGGACAT CATGTGGTAC AAGGATGAGG CGCTGCTGAC CGAGAGCAAC
    CATGTGAGCT TCGTGTACGA GGAGAACGAG TGCTCCCTGG TGGTGCTCAG CGCGGGGGCC
    CAGGATGGAG GGGTCTACAC CTGCACGGCC CGTAACCTGG CTGGCGAAGT CTCCTGCAAA
    GCAGAGCTGG CTGTGCACTC AGCTCAGACA GCTATGGAGG TGGAAGGGAT CGGGGAAGAC
    GAGGAGCAGC GAGGAAGGAG GCTCAGCGAC TTCTATGATA TTCAGCAGGA GATCGGCAGG
    GGTGCCTTCT CCTACCTGCG CCGTGTAGTG GAGAGGAGCT CCGGACTGGA GTTTGCAGCC
    AAGTTCATCC CCAGCCAGGC AAAGCCAAAG GCATCGGCAT GGCGGGAGGC ACGGCTGCTG
    GCCCGGCTCC AGCATGACTG CATCCTCTAC TTCCATGAGG CCTTCGAGAG GCGCCGGGGA
    CTGGTCATCG TCACCGAGCT CTGCACAGAA GAGCTTCTGG AGCGAATGGC CAGGAAACCC
    ACCGTGTGTG AGTCTGAGAT CCGGGCCTAC ATGCGGCAGG TACTAGAAGG AATATGCTAC
    TTGCACGAGA ACCACGTGCT GCACCTGGAT GTCAAGCCCG AGAACCTGCT GGTGTGGGAT
    GGTGTTGAGG GCGAGGAGCA GGTGAGGATC TGTGACTTTG GGAACGCTCA AGAGCTGACA
    CCGGGAGAGC CCCAGTATTG CCAATACGGC ACACCTGAGT TCGTGGCGCC CGAGATTGTC
    AACCAGACCC CTGTGTCTGG AGTCACTGAC ATCTGGCCCG TGGGTGTCGT CGCCTTCCTC
    TGCCTGACCG GAATCTCCCC GTTCGTTGGG GAAAATGATC GGACGACATT GATGAACATC
    CGAAACTACA ACGTGGCCTT CGAGGAGACC ACGTTCCTGA GCCTGAGCAG GGAGGCCCGG
    GGCTTCCTCA TCAAAGTGCT GGTGCGGGAC CGGCTGAGGC CTACTGCAGA GGAGACCCTG
    GAACATCCTT GGTTTAAAGC ACAGGCAAAG GGTGCAGTGG TGAGCACGGA TCACCTAAAG
    CTATTCCTCT CCCGGCGGAG GTGGCAGCGC TCCCAGATCA GCTACAAGTG CCACCTGGTG
    CTGCGCCCCA TCCCGGAGCT GCTGCGAGCA CCCCCGGAGC GGGTGTGGGT GGCCGTGCCC
    CGAAGACCAC CCCCTAGTGG AGGGCTGTCG TCATCATCCG ACTCTGAAGA GGAAGAGCTG
    GAGGAGCTGC CCTCTGTGCC CCGACCTCTG CAGCCTGAGT TTTCGGGCTC CCGGGTGTCC
    CTCACTGACA TTCCCACTGA GGATGAGGCC CTCGGGGCCC TGGAGGCTGG GGCGGCCACC
    CCCATGGACT GGCAAGAACA AGGAAAGGCC CCCTCTCAGG ACCAGGAGGC CCCCAGCCCT
    CAGGCACTCC CCTCCCCAGG CCAGGAGTCC CCGTCCGGGC CCAGCCCCCG GCGGGCAGAG
    CTCCGCAGGG GCAGCTCGGC TGAGAGCGCC CTGCCCCGGG CCGGGCCGAG GGAACCGGGC
    CGGGGCCTGC ACAAGGCAGC GTCCGTCGAG CTGCCGCAGC GCCGAAGCCC CAGCCCGGGG
    GCCACCCGCC TGACCCGAGG AGGCCTGGGT GAGGGCGAGT ATGCCCAGAG GCTGCAGGCC
    CTGCGCCAGC GGCTGCTGAG GGGTGGCCCC GATGATGGCA AGGTCAGCGG CCTCAGGGGC
    CCCCTGCTGG AGAGCCTGGG GGGCCGGGCT CGTGACCCCC GGCTGGCGCG GGCAGCCTCC
    AGCGAGGCAG CACCCCATCA CCAGCACCCA CCTGAGGCTC GGGGCCTGCA GAAGAGCAGT
    AGCTTCTCGC AGGGTGAGGC AGAGCCCCGG GGCCGGCACC GCCGAGCAGG GGCACCCCTT
    GAGATCCCCG TGGCCCGGCT GGGGGCCCGC AAGCTGCAGG AGTCTCCCTC CTTGTCCGCC
    CTCAGCGAGG CCCAACCACC TAGCCCCGTG CGGCCCAGTG CAGCCAAGCC CAGCACCCCT
    AAGTCTTCAG AACCTCTTGT GGGCACCCCC AGTGAGGCTG CACAGCCTCC AGCACCCCAG
    CCTGCCCGAG AGAAGGCCCC AGAATCTACG CCAGAACCAG CTCGGGGCCC CAAGCCCACA
    CAGCCCCCCA CAGCTCTGCA AACCCTAGCG CCTCCTCTCA CTCCGTATGC GCAGATCATG
    CAGTCGCTCC AGCTGCCAGG CCAGGCGCAA GGCCCCCCAC AGGGCGCAGT CGTATCTCCT
    ACATCTCGGT CTCCAGCATC TCGGTTGGAA CCCAAGCCCC ACCCGGCTGT GTTCTCCAGG
    GTGGCCTCCC CACCTCCAGG AGCCTCCGAG AAGCGCCTGC TGCCGGGAGC CGGAGCAACC
    CCAGGGCCAG CCGAAAAAGC CCGGGTCCCG GCGGTCCCCC GCAGGCCAGG GAGCAGTCTC
    AGCAGCAGCA TCGAGAACCT GGAGTCGGAG GCCGTGTTCG AGGCCAAGTT CAAGCGCAGC
    CGGGAGTCGC CCCTGTCGCG GGGGCTGCGG CTGCTGAGCC GCTCGCGGTC GGAGGAGCGC
    GGCCCCTTCC GCGGGGCTGA GGAGGAGGAC GGCATTTACC GGCCCAGCCC GGCGGGCACG
    CCGCTGGAGC TGGTGCGACG GCCCGAGCGC TCGCGCTCCG TGCAGGACCT CAGGGCGGTC
    GGGGAGCCAG GCCTCGTGCG CCGCCTCTCG CTGTCGCTGT CCCAGCGTCT GCGGCGGACC
    CCGCCCGCGC AGCGCCACCC GGCCTGGGAG CCCCGCGGCG GGGACGGCGA GAGCTCGGAG
    GGCGGCAGCT CGGCGCGGGG CTCCCCGGTG CTGACGGTGC GCCGGAGGCT CAGCTCCACG
    CTGGAGCGGC TGTCGAGCCG GTTGCAGCGC AGCGGCAGCA GCGAGGACTC GGGGGGCGCG
    TCGGGCCGCA GCACGCCGCT GTTCGGCCGG CTGCGCAGGG CCACGTCCGA GAGCGAGAGT
    CTGCGGCGCC TGGACCTCGC GCAAAATCAG CTGGCGGCCC AAGCCGGCGC CGCCACCCCT
    TCCGCGGAGT CCCTGGGCTC CGAGGCCAGC GCTACGTCGG GCTCCTCTGC TCCTGCAGAA
    AGTCGAAGCC GCCTGCGCTG GGGCCTCTCT CGGCTGCGGA AGGACAAGAG GTTGTCACAG
    CCTAACCTCT CTGGCAGTGT CCAGGAGGAT TTGGGTCACC AGTATGTGCG CAGTGAGTCA
    GACTTTCCCC CAGTCTTCCA CATCAAACTC AAGGACCAGG TGCTGCTGGA GGGAGAGGCA
    GCCACCCTGC TCTGCTTGCC GGCAGCCTGC CCTGCACCCC ACATCTCCTG GATGAAAGAC
    AAGCAGTCCC TGCGGTCAGA GCCCTCGGTG ATCATCGTGT CCTGCAAAGA TGGTCGTCAG
    CTACTGAGCA TCCCCCGGGC AGGCAAGCGG CATGCGGGGC TCTATGAGTG CTCCGCCACC
    AACGTCCTTG GCAGCATCAC CAGCTCCTGT ACCGTGGCTG TGGCTCGTAC CCCAGGAAAG
    CTAGCTCCTC CTGAGGTGCC CCAGACCTAC CAGGACACAG CACTGGTGCT CTGGAAGCCG
    GGAGACAGCA AGGCGCCTTG CACATACACG CTGGAGCGGC GGGTGGATGG GGAGTCTTCC
    TGGCACCCTG TGAGCTCGGG CATCCCCGAC TGCTACTACA ACGTGACCCA CCTACCAGTC
    GGCATGGCTG TGAGGTTCCG TGTGGCCTGT GCCAACCGTG CTGGGCAAGG GCCTTTCAGC
    AACCCTTCTG AGAAGGTCCT CGTCAGGGGC GTGCAAGATT CCTCAGCTCT GCCATCTGCT
    GCTCACAGAG ATGCCCCTGT TACCTCAGGG CCAGCCAGGG CCCCGCCTCC TGACGCTCCT
    ACCTCACTGG CCCCTCCCCT GAGCCCCTCC CCAGCTCCTG CTCCCCCAGG CCCCCAGGCA
    GCCCCTCTCG GCCCTTCATC TTCCCCAGCA CCCCCCAGCC AGGCCTTGTC CTCCCTCAAG
    GCTGTGGGTC CACCACCCCA AACCCCCCCA CGAAAGCACC GGGGCCTGCA GGCTGCCCAG
    CAAGTAGAAC CTACCCCACC CAGTGCCCAG GACTCCCCAA GTGAGCCCAA GTCTTCCGTC
    CCTGACACTG GGACCCCCAT CCCAGCCTCT GCCCCTCAAG TGGTGAAAAC AGCCTCTTCC
    TCAATGCCCC TGTATATGGT GACCTCCTTT GTGTCTGCAC CACCAGCCCC TGAGCCCCCA
    GCCCCCGAGC CCCCTCCTGA GCCCACCAAA GTGACTGTGC GGAGCCTCAG CCCAGCCAAG
    GAGGTGGTTG GCTCCCCTGG GGGTGGTCCC CCCAGGGCTC CTAAGCCAGA GGGCACCACT
    CTTCGACAGG GCCCTCCTCA GAAACCATAC ACCTTCCTGG AGGAGAAGGC CAGGGGTCGC
    TTTGGTGTGG TGCGTGCCTG CCGGGAGAAT GCCACCGGGC GAACATTCGT GGCCAAGATT
    GTGCCATACG CGGCCGAGGG CAAGCGGCAG GTCCTGCAGG AGTACGAGGT GCTGAGGACC
    CTGCACCACG AGCGGCTTAT GGCCCTGCAC GAAGCCTACA TCACCCCGCG CTACCTCGTG
    CTCATCGCCG AGAGCTGCGG CAACCGGGAG CTTCTCTGTG GGCTCAGCGA CAGATTCCGG
    TATTCAGAGG ATGATGTGGC CACCTATGTG GTGCAGCTGC TACAAGGCCT GGATTACCTC
    CATGGCCGCC ACGTGCTGCA CCTGGACATC AAGCCAGACA ACCTGCTGCT GGCCCCTGAC
    AACGCCCTCA AGATCGTGGA CTTTGGCAGT GCCCAGCCCT ACAACCCCCA GGCTCTGCGG
    CCCCTGGGTC ACCGCACGGG CACACTGGAG TTCATGGCTC CTGAGATGGT AAAGGGAGAC
    CCCATCGGCT CTGCCACGGA CATCTGGGGA GCGGGTGTGC TCACTTACAT CATGCTCAGT
    GGACGCTCCC CATTCTATGA GCCAGACCCC CAGGAAACAG AGGCTCGCAT TGTGGGGGGC
    CGCTTTGATG CCTTCCAGCT GTATCCCAAC ACATCCCAGA GTGCCACCCT CTTCTTGCGA
    AAGGTCCTCT CAGTACATCC CTGGAGCCGG CCCTCCGTGC AGGACTGCCT GGCTCACCCG
    TGGCTGCAGG ATGCCTACCT GATGAAACTG CGCCGCCAGA CACTCACCTT CACCACCAAC
    CGGCTCAAGG AGTTCCTGGG TGAGCAGCGG CGCCGCAGGG CCGAGGCCGC CACCCGCCAC
    AAGGTGCTGC TCCGCTCTTA CCCAGGCAGC CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50732
    Clone ID Related Accession (Same CDS sequence) XM_010802350.2
    Accession Version XM_010802350.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product striated muscle preferentially expressed protein kinase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103849.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802350.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    9001
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    9181
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    9301
    9361
    9421
    9481
    ATGTCCCCTG CACCATCCAT CCCTCTGTGC ACTTCTCCAC CCCCCCACCA TTGCTTCCTC 
    TCCTGGCCCC AGCTGCCCAC TCTGTCTTCC TACACAGCTC CATCCTCCTC CTGTTCCATC
    ACCACCCACA TGCTCCTGCT CCCACCTGTC CTGGCTCACT GTGCCATCTC CATGGTCTCG
    TGGACCCCTA TCTCCCTTCC TTGTCTCCCG CAAGATCTCC ACTCTCCCGG GGGCCCTCTC
    CTGCCTCGCC CATTCAGGCT CCAGTTGTCC CCAGGATCCC CACTCCCCCC CCCATCAGGG
    GCCCCCTCCG TGCCTGCTGT CTCAGCAGCT GCTGCCTTTT CATCTCTGCA CATTCCTGTT
    CCCATGTGGG CCTTTTTCTG GGAGGAACGG AACCTTTCTG CACGGCAGCT CCCGGGAGAG
    CAGGAGGGAG CGAGCGAGAG CACAACCAGG AGACAGAAGA GAGCGAGCAG TGTCCCTCAG
    AGCGGACTGC ACAAGGAGGA GCCTGACCTT CAGCCTCAGC CCGCCAGCGA AGCCCCCCGT
    CGCCCAGCCC TGCCGCCCCC CTCCAAATCC GCGCTGCTGC CCCCACCGTC CCCTCGGGTG
    GGTAAGCGGC CCCCGCCGGG AGCCGGCGGC CAGCCCCTGG CCACCCCCAC GCCGCCGCAC
    CGGCGCTCTC GGGAGCCGGT GCCGCCCGAG GACGCCACCG CCGAAGAAAA GCGAGGGAAA
    AAGTCCAAGT CGTCCGGGCC CTCGCTAGCG GGCACGGCGG AGTCCCGGCC CCAGACGCCC
    CTGAGTGAGG CCTCGGGCCG CCAGTCGGCG CTGGGCCGCT CCCCGAGGCT GGTGCGCGCT
    GGCTCCCGCA TCCTGGACAA GCTGCAGTTC TTCGAGGAGC GCCGGCGCAG CCTGGAGCGC
    AGCGACTCGC CGCCGGCGCC CCTGCGGCCC TGGGTGCCCC TGCGCAAGGC CCGTTCGCTG
    GAGCAGCCCA AGTCCGAGGG CGGCGGGCCG TGGGGCACGC CCAGGGCCTC GCAGGAGGAG
    CTGCGGTCCC CGGCGGGCAG CGTGGCCGAG CGGCGCCGCC TATTCCAACA GAAGGCGGCC
    TCGCTGGACG AGCGCACGCG TCAGCGCAGC CCTGCGTCCG ACCTCGAGCT GCGCTTCGCC
    CAGGAGCTGG GCCGCATCCG CCGCTCCACG TCGCGGGAGG AGCTGGTGCG CTCTCACGAG
    TCCCTGCGCG CCACGCTGCA GCGCGCCCCG TCCCCCCGAG AGCCCGGCGA GCCGCCGCTC
    TTCTCCCAGC CCTCCACCCC CAAGACCCCG CGGGCCTTGA GCCCCGCCGC CGCCCAGCCG
    CCCCCTGCGA GCGTCGCGGA AAAGCCCGGG GATGAGCCAG GGAGGCCCCG GGGCCGTGGG
    CCAGCGGGCA GGACGGAGCC GGGGGAAGGC CCGCAGCAGG AGGTTAGGCG CCGGGACCAG
    TTCCCGCTGA CCCGGAGCAG AGCCATCCAG GAGTGCCGGA GCCCGGTGCC GCCCCCCACC
    TCTGAGCCCC CCGAGACCAG GACGAAAGCT CCCCCGGGTC GGAAGCGGGA GCCCCCGCCG
    CAGGCCGTGC GCTTCCTGCC CTGGGCCACG CCGGGCCAGG AGGGCGCTGC TGTGCCCCAG
    ACCTTGGAGA AGAACAGGGC GGGACCCGAG GCCGAGAAGA GGCTGCGCAG AGGGCCAGAG
    GAGGACGGTC CTTGGGGGGC CTGGGACCGC CGAGGGGCCC GCAGCCAGGG CAGAGGTCGC
    CGGGCGCGGC CCACCTCGCC TGAGCTCGAG TCTTCGGATG ACTCCTATGT GTCTGCTGGA
    GAAGAGCCTC TGGAGGCCCC TGTGTTTGAG ATCCCCCTGC AAAATGCAGT GGTGGCCCCA
    GGGGTGGACG TGCTGCTCAA GTGTATTGTT ACCGCCAACC CCCCGCCCCA AGTGTCCTGG
    CAGAAGGATG GGTCCCCGCT GCGCAGTGAT GGCCGCCTTC TCATCCGGGC AGAAGGCGAG
    CGGCACACCC TGCTGCTCCG GGAGGCCCGG GCCGCCGATG CCGGGAGCTA TGCAGCCACT
    GCCACCAACG AGCTGGGCCA GGCCCGCTGC GCTGCCACGC TGGCCGTGAG ACCTGGTGGG
    TCAACATCTC CTTTCAGCAG TCCTATCACC TCTGACGAGG AGTATCTGAG CCCCCCAGAG
    GAGTTCCCGG AACCCGGGGA GACCTGGCCC CGAGCCCCCA CCATGAAGCT CAGTCCTGGC
    CAGAATCGCC GTTCCTCCGA CACTGGCTCC AAGGCGCCCC CTACTTTCAA GGTTTCACTT
    ATGGACCAGT CAGTAAGGGA AGGCCAAGAC GTCACCATGA GCATCCGTGT GCAGGGCGAA
    CCCAAGCCGG TGGTCTCCTG GCTGAGAAAC CGTCAGCCTG TGCGCCCAGA CCAGCGGCGC
    TTTGCTGAGG AGGCAGAGGA TGGGCTCTGC CGGCTTCGGA TCCTGGCTGC CGAGCGGGGC
    GACGCTGGCT TCTACACCTG CAAGGCGGTT AACGAGTATG GCGCTCGACA GTGTGAGGCG
    CGCCTGGAGG TCAGAGCACA CCCTGAAAGC CGGTCCCTGG CCGTGCTGGC CCCTCTGCAG
    GACGTGGACG TGGGGGCCGG GGAGATGGCG CTGTTTGAGT GCCTGGTGGC GGGTCCCACC
    GACGTGGAGG TAGACTGGCT GTGCCGAGGC CGCCTGCTGC AGCCTGCACT ACTCAAATGC
    AAGATGCATT TCGATGGCCG CAAATGCAAG CTGCTGCTCA CCTCTGTGCA TGAGGACGAC
    AGCGGCGTCT ACACCTGCAA GCTCAGCACG GCCAAAGATG AACTGACCTG CAGTGCCCGG
    CTGACCGTGC GACCCTCGCT GGCACCCCTG TTCACGCGGC TGCTGGAGGA CGTGGAAGTG
    CTGGAGGGCC GCGCTGCCCG CTTCGACTGC AAGATCAGCG GGAGCCCGCC CCCCTCCGTT
    ACCTGGACTC ACTTTGGCCA CCCCGTGGAG GAGAGCGAGA ACGTGCGGCT GCGGCAGGAC
    GGGGGCCTGC ATTCGCTGCA CATCGCACAT GTGGGCAGTG AGGATGAGGG GCTGTACGCG
    GTCAGTGCTG CCAACACCCA CGGCCAGGCC CACTGCTCAG CCCAGCTCTA CGTGGAGGAG
    CCTCGGACGG CTGCCACCGG CCCTAGCTCC AAGCTGGAGA AGATGCCCTC CATCCCCGAG
    GAGCCCGAGC AGGGTGAGCT GGAGCGGCTG TCCATGCCCG ACTTCCTGCG GCCGCTGCAG
    GATCTGGACG TGGGACTGGC CAAGGAGGCC ATGCTGGAGT GCCAGGTGAC CGGCCTGCCC
    TACCCCACCA TCAGCTGGTT CCACAACGGC CACCGCATCC AGAGCAGCGA CGACCGACGC
    ATGACGCAGT ACAGGGATGT CCATCGCTTG GTGTTCCCTG CCGTGGGACC TCAGCATGCT
    GGTGTCTACA AGAGCGTCAT TGCTAACAAG CTGGGCAAAG CCGCCTGCTA TGCTCATCTC
    TACGTCACAG ATGTGGTTCC AGGCCCCCCA GACGGTGCCC CTCAGGTAGT GGCTGTGACC
    GGGAGGATGA TCACGCTTGC ATGGAACCCC CCGAGGAGTC TGGACATGGC TATCGACCCA
    GACGCCCTGA CCTACACCGT GCAGCACCAG GTGCTGGGCT CAGACCAGTG GACGGCGCTG
    GTCACGGGCC TGCGGCAGCC CGGGTGGGCA GCCACCGGAT TGCGAAAGGG GGTCCAGCAC
    GTCTTCCGGG TTCTCAGCAC CACCATCAAG AGCAGCAGCA AGCCCTCGCC CCCGTCCGAG
    CCCACGCAGC TGCTGGAGCG TGGCCCGCCC CTGGAGGAGG CGCCCGCTGT GCTGGACAAG
    CCAGACGTCG TGTATGTGGT GGAGGGCCAG CCCACCAGTG TCACCGTCAC CTTCAACCAC
    GTGGAGGCCC AGGTTGTCTG GAGGAGCTGC CGAGGAGCCC TCCTAGAGGC CCGGCCAGGT
    GTGTTTGAGC TGAGCCAGCC AGATGACGAC CAGTACTCTC TTAGGATCTG CCGGGTGAGC
    CGCCGGGACC TGGGAGCCCT CACTTGCACT GCCCGCAACC GCCACGGCAC ACAGGCCTGC
    TCAGTCACCC TGGAGCTGGC AGAGGCCCCT CGGTTTGAGT CCATCATGGA GGATGTGGAG
    GTAGGGGCTG GAGAGACTGC TCGCTTCGCC GTGGTGGTGG ACGGGAAGCC CTTGCCGGAC
    ATCATGTGGT ACAAGGATGA GGCGCTGCTG ACCGAGAGCA ACCATGTGAG CTTCGTGTAC
    GAGGAGAACG AGTGCTCCCT GGTGGTGCTC AGCGCGGGGG CCCAGGATGG AGGGGTCTAC
    ACCTGCACGG CCCGTAACCT GGCTGGCGAA GTCTCCTGCA AAGCAGAGCT GGCTGTGCAC
    TCAGCTCAGA CAGCTATGGA GGTGGAAGGG ATCGGGGAAG ACGAGGAGCA GCGAGGAAGG
    AGGCTCAGCG ACTTCTATGA TATTCAGCAG GAGATCGGCA GGGGTGCCTT CTCCTACCTG
    CGCCGTGTAG TGGAGAGGAG CTCCGGACTG GAGTTTGCAG CCAAGTTCAT CCCCAGCCAG
    GCAAAGCCAA AGGCATCGGC ATGGCGGGAG GCACGGCTGC TGGCCCGGCT CCAGCATGAC
    TGCATCCTCT ACTTCCATGA GGCCTTCGAG AGGCGCCGGG GACTGGTCAT CGTCACCGAG
    CTCTGCACAG AAGAGCTTCT GGAGCGAATG GCCAGGAAAC CCACCGTGTG TGAGTCTGAG
    ATCCGGGCCT ACATGCGGCA GGTACTAGAA GGAATATGCT ACTTGCACGA GAACCACGTG
    CTGCACCTGG ATGTCAAGCC CGAGAACCTG CTGGTGTGGG ATGGTGTTGA GGGCGAGGAG
    CAGGTGAGGA TCTGTGACTT TGGGAACGCT CAAGAGCTGA CACCGGGAGA GCCCCAGTAT
    TGCCAATACG GCACACCTGA GTTCGTGGCG CCCGAGATTG TCAACCAGAC CCCTGTGTCT
    GGAGTCACTG ACATCTGGCC CGTGGGTGTC GTCGCCTTCC TCTGCCTGAC CGGAATCTCC
    CCGTTCGTTG GGGAAAATGA TCGGACGACA TTGATGAACA TCCGAAACTA CAACGTGGCC
    TTCGAGGAGA CCACGTTCCT GAGCCTGAGC AGGGAGGCCC GGGGCTTCCT CATCAAAGTG
    CTGGTGCGGG ACCGGCTGAG GCCTACTGCA GAGGAGACCC TGGAACATCC TTGGTTTAAA
    GCACAGGCAA AGGGTGCAGT GGTGAGCACG GATCACCTAA AGCTATTCCT CTCCCGGCGG
    AGGTGGCAGC GCTCCCAGAT CAGCTACAAG TGCCACCTGG TGCTGCGCCC CATCCCGGAG
    CTGCTGCGAG CACCCCCGGA GCGGGTGTGG GTGGCCGTGC CCCGAAGACC ACCCCCTAGT
    GGAGGGCTGT CGTCATCATC CGACTCTGAA GAGGAAGAGC TGGAGGAGCT GCCCTCTGTG
    CCCCGACCTC TGCAGCCTGA GTTTTCGGGC TCCCGGGTGT CCCTCACTGA CATTCCCACT
    GAGGATGAGG CCCTCGGGGC CCTGGAGGCT GGGGCGGCCA CCCCCATGGA CTGGCAAGAA
    CAAGGAAAGG CCCCCTCTCA GGACCAGGAG GCCCCCAGCC CTCAGGCACT CCCCTCCCCA
    GGCCAGGAGT CCCCGTCCGG GCCCAGCCCC CGGCGGGCAG AGCTCCGCAG GGGCAGCTCG
    GCTGAGAGCG CCCTGCCCCG GGCCGGGCCG AGGGAACCGG GCCGGGGCCT GCACAAGGCA
    GCGTCCGTCG AGCTGCCGCA GCGCCGAAGC CCCAGCCCGG GGGCCACCCG CCTGACCCGA
    GGAGGCCTGG GTGAGGGCGA GTATGCCCAG AGGCTGCAGG CCCTGCGCCA GCGGCTGCTG
    AGGGGTGGCC CCGATGATGG CAAGGTCAGC GGCCTCAGGG GCCCCCTGCT GGAGAGCCTG
    GGGGGCCGGG CTCGTGACCC CCGGCTGGCG CGGGCAGCCT CCAGCGAGGC AGCACCCCAT
    CACCAGCACC CACCTGAGGC TCGGGGCCTG CAGAAGAGCA GTAGCTTCTC GCAGGGTGAG
    GCAGAGCCCC GGGGCCGGCA CCGCCGAGCA GGGGCACCCC TTGAGATCCC CGTGGCCCGG
    CTGGGGGCCC GCAAGCTGCA GGAGTCTCCC TCCTTGTCCG CCCTCAGCGA GGCCCAACCA
    CCTAGCCCCG TGCGGCCCAG TGCAGCCAAG CCCAGCACCC CTAAGTCTTC AGAACCTCTT
    GTGGGCACCC CCAGTGAGGC TGCACAGCCT CCAGCACCCC AGCCTGCCCG AGAGAAGGCC
    CCAGAATCTA CGCCAGAACC AGCTCGGGGC CCCAAGCCCA CACAGCCCCC CACAGCTCTG
    CAAACCCTAG CGCCTCCTCT CACTCCGTAT GCGCAGATCA TGCAGTCGCT CCAGCTGCCA
    GGCCAGGCGC AAGGCCCCCC ACAGGGCGCA GTCGTATCTC CTACATCTCG GTCTCCAGCA
    TCTCGGTTGG AACCCAAGCC CCACCCGGCT GTGTTCTCCA GGGTGGCCTC CCCACCTCCA
    GGAGCCTCCG AGAAGCGCCT GCTGCCGGGA GCCGGAGCAA CCCCAGGGCC AGCCGAAAAA
    GCCCGGGTCC CGGCGGTCCC CCGCAGGCCA GGGAGCAGTC TCAGCAGCAG CATCGAGAAC
    CTGGAGTCGG AGGCCGTGTT CGAGGCCAAG TTCAAGCGCA GCCGGGAGTC GCCCCTGTCG
    CGGGGGCTGC GGCTGCTGAG CCGCTCGCGG TCGGAGGAGC GCGGCCCCTT CCGCGGGGCT
    GAGGAGGAGG ACGGCATTTA CCGGCCCAGC CCGGCGGGCA CGCCGCTGGA GCTGGTGCGA
    CGGCCCGAGC GCTCGCGCTC CGTGCAGGAC CTCAGGGCGG TCGGGGAGCC AGGCCTCGTG
    CGCCGCCTCT CGCTGTCGCT GTCCCAGCGT CTGCGGCGGA CCCCGCCCGC GCAGCGCCAC
    CCGGCCTGGG AGCCCCGCGG CGGGGACGGC GAGAGCTCGG AGGGCGGCAG CTCGGCGCGG
    GGCTCCCCGG TGCTGACGGT GCGCCGGAGG CTCAGCTCCA CGCTGGAGCG GCTGTCGAGC
    CGGTTGCAGC GCAGCGGCAG CAGCGAGGAC TCGGGGGGCG CGTCGGGCCG CAGCACGCCG
    CTGTTCGGCC GGCTGCGCAG GGCCACGTCC GAGAGCGAGA GTCTGCGGCG CCTGGACCTC
    GCGCAAAATC AGCTGGCGGC CCAAGCCGGC GCCGCCACCC CTTCCGCGGA GTCCCTGGGC
    TCCGAGGCCA GCGCTACGTC GGGCTCCTCT GCTCCTGCAG AAAGTCGAAG CCGCCTGCGC
    TGGGGCCTCT CTCGGCTGCG GAAGGACAAG AGGTTGTCAC AGCCTAACCT CTCTGGCAGT
    GTCCAGGAGG ATTTGGGTCA CCAGTATGTG CGCAGTGAGT CAGACTTTCC CCCAGTCTTC
    CACATCAAAC TCAAGGACCA GGTGCTGCTG GAGGGAGAGG CAGCCACCCT GCTCTGCTTG
    CCGGCAGCCT GCCCTGCACC CCACATCTCC TGGATGAAAG ACAAGCAGTC CCTGCGGTCA
    GAGCCCTCGG TGATCATCGT GTCCTGCAAA GATGGTCGTC AGCTACTGAG CATCCCCCGG
    GCAGGCAAGC GGCATGCGGG GCTCTATGAG TGCTCCGCCA CCAACGTCCT TGGCAGCATC
    ACCAGCTCCT GTACCGTGGC TGTGGCTCGT ACCCCAGGAA AGCTAGCTCC TCCTGAGGTG
    CCCCAGACCT ACCAGGACAC AGCACTGGTG CTCTGGAAGC CGGGAGACAG CAAGGCGCCT
    TGCACATACA CGCTGGAGCG GCGGGTGGAT GGGGAGTCTT CCTGGCACCC TGTGAGCTCG
    GGCATCCCCG ACTGCTACTA CAACGTGACC CACCTACCAG TCGGCATGGC TGTGAGGTTC
    CGTGTGGCCT GTGCCAACCG TGCTGGGCAA GGGCCTTTCA GCAACCCTTC TGAGAAGGTC
    CTCGTCAGGG GCGTGCAAGA TTCCTCAGCT CTGCCATCTG CTGCTCACAG AGATGCCCCT
    GTTACCTCAG GGCCAGCCAG GGCCCCGCCT CCTGACGCTC CTACCTCACT GGCCCCTCCC
    CTGAGCCCCT CCCCAGCTCC TGCTCCCCCA GGCCCCCAGG CAGCCCCTCT CGGCCCTTCA
    TCTTCCCCAG CACCCCCCAG CCAGGCCTTG TCCTCCCTCA AGGCTGTGGG TCCACCACCC
    CAAACCCCCC CACGAAAGCA CCGGGGCCTG CAGGCTGCCC AGCAAGTAGA ACCTACCCCA
    CCCAGTGCCC AGGACTCCCC AAGTGAGCCC AAGTCTTCCG TCCCTGACAC TGGGACCCCC
    ATCCCAGCCT CTGCCCCTCA AGTGGTGAAA ACAGCCTCTT CCTCAATGCC CCTGTATATG
    GTGACCTCCT TTGTGTCTGC ACCACCAGCC CCTGAGCCCC CAGCCCCCGA GCCCCCTCCT
    GAGCCCACCA AAGTGACTGT GCGGAGCCTC AGCCCAGCCA AGGAGGTGGT TGGCTCCCCT
    GGGGGTGGTC CCCCCAGGGC TCCTAAGCCA GAGGGCACCA CTCTTCGACA GGGCCCTCCT
    CAGAAACCAT ACACCTTCCT GGAGGAGAAG GCCAGGGGTC GCTTTGGTGT GGTGCGTGCC
    TGCCGGGAGA ATGCCACCGG GCGAACATTC GTGGCCAAGA TTGTGCCATA CGCGGCCGAG
    GGCAAGCGGC AGGTCCTGCA GGAGTACGAG GTGCTGAGGA CCCTGCACCA CGAGCGGCTT
    ATGGCCCTGC ACGAAGCCTA CATCACCCCG CGCTACCTCG TGCTCATCGC CGAGAGCTGC
    GGCAACCGGG AGCTTCTCTG TGGGCTCAGC GACAGATTCC GGTATTCAGA GGATGATGTG
    GCCACCTATG TGGTGCAGCT GCTACAAGGC CTGGATTACC TCCATGGCCG CCACGTGCTG
    CACCTGGACA TCAAGCCAGA CAACCTGCTG CTGGCCCCTG ACAACGCCCT CAAGATCGTG
    GACTTTGGCA GTGCCCAGCC CTACAACCCC CAGGCTCTGC GGCCCCTGGG TCACCGCACG
    GGCACACTGG AGTTCATGGC TCCTGAGATG GTAAAGGGAG ACCCCATCGG CTCTGCCACG
    GACATCTGGG GAGCGGGTGT GCTCACTTAC ATCATGCTCA GTGGACGCTC CCCATTCTAT
    GAGCCAGACC CCCAGGAAAC AGAGGCTCGC ATTGTGGGGG GCCGCTTTGA TGCCTTCCAG
    CTGTATCCCA ACACATCCCA GAGTGCCACC CTCTTCTTGC GAAAGGTCCT CTCAGTACAT
    CCCTGGAGCC GGCCCTCCGT GCAGGACTGC CTGGCTCACC CGTGGCTGCA GGATGCCTAC
    CTGATGAAAC TGCGCCGCCA GACACTCACC TTCACCACCA ACCGGCTCAA GGAGTTCCTG
    GGTGAGCAGC GGCGCCGCAG GGCCGAGGCC GCCACCCGCC ACAAGGTGCT GCTCCGCTCT
    TACCCAGGCA GCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800652.1
    CDS88..9585
    Translation

    Target ORF information:

    RefSeq Version XM_010802350.2
    Organism Bos taurus(cattle)
    Definition Bos taurus SPEG complex locus (SPEG), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802350.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGTCCCCTG CACCATCCAT CCCTCTGTGC ACTTCTCCAC CCCCCCACCA TTGCTTCCTC 
    TCCTGGCCCC AGCTGCCCAC TCTGTCTTCC TACACAGCTC CATCCTCCTC CTGTTCCATC
    ACCACCCACA TGCTCCTGCT CCCACCTGTC CTGGCTCACT GTGCCATCTC CATGGTCTCG
    TGGACCCCTA TCTCCCTTCC TTGTCTCCCG CAAGATCTCC ACTCTCCCGG GGGCCCTCTC
    CTGCCTCGCC CATTCAGGCT CCAGTTGTCC CCAGGATCCC CACTCCCCCC CCCATCAGGG
    GCCCCCTCCG TGCCTGCTGT CTCAGCAGCT GCTGCCTTTT CATCTCTGCA CATTCCTGTT
    CCCATGTGGG CCTTTTTCTG GGAGGAACGG AACCTTTCTG CACGGCAGCT CCCGGGAGAG
    CAGGAGGGAG CGAGCGAGAG CACAACCAGG AGACAGAAGA GAGCGAGCAG TGTCCCTCAG
    AGCGGACTGC ACAAGGAGGA GCCTGACCTT CAGCCTCAGC CCGCCAGCGA AGCCCCCCGT
    CGCCCAGCCC TGCCGCCCCC CTCCAAATCC GCGCTGCTGC CCCCACCGTC CCCTCGGGTG
    GGTAAGCGGC CCCCGCCGGG AGCCGGCGGC CAGCCCCTGG CCACCCCCAC GCCGCCGCAC
    CGGCGCTCTC GGGAGCCGGT GCCGCCCGAG GACGCCACCG CCGAAGAAAA GCGAGGGAAA
    AAGTCCAAGT CGTCCGGGCC CTCGCTAGCG GGCACGGCGG AGTCCCGGCC CCAGACGCCC
    CTGAGTGAGG CCTCGGGCCG CCAGTCGGCG CTGGGCCGCT CCCCGAGGCT GGTGCGCGCT
    GGCTCCCGCA TCCTGGACAA GCTGCAGTTC TTCGAGGAGC GCCGGCGCAG CCTGGAGCGC
    AGCGACTCGC CGCCGGCGCC CCTGCGGCCC TGGGTGCCCC TGCGCAAGGC CCGTTCGCTG
    GAGCAGCCCA AGTCCGAGGG CGGCGGGCCG TGGGGCACGC CCAGGGCCTC GCAGGAGGAG
    CTGCGGTCCC CGGCGGGCAG CGTGGCCGAG CGGCGCCGCC TATTCCAACA GAAGGCGGCC
    TCGCTGGACG AGCGCACGCG TCAGCGCAGC CCTGCGTCCG ACCTCGAGCT GCGCTTCGCC
    CAGGAGCTGG GCCGCATCCG CCGCTCCACG TCGCGGGAGG AGCTGGTGCG CTCTCACGAG
    TCCCTGCGCG CCACGCTGCA GCGCGCCCCG TCCCCCCGAG AGCCCGGCGA GCCGCCGCTC
    TTCTCCCAGC CCTCCACCCC CAAGACCCCG CGGGCCTTGA GCCCCGCCGC CGCCCAGCCG
    CCCCCTGCGA GCGTCGCGGA AAAGCCCGGG GATGAGCCAG GGAGGCCCCG GGGCCGTGGG
    CCAGCGGGCA GGACGGAGCC GGGGGAAGGC CCGCAGCAGG AGGTTAGGCG CCGGGACCAG
    TTCCCGCTGA CCCGGAGCAG AGCCATCCAG GAGTGCCGGA GCCCGGTGCC GCCCCCCACC
    TCTGAGCCCC CCGAGACCAG GACGAAAGCT CCCCCGGGTC GGAAGCGGGA GCCCCCGCCG
    CAGGCCGTGC GCTTCCTGCC CTGGGCCACG CCGGGCCAGG AGGGCGCTGC TGTGCCCCAG
    ACCTTGGAGA AGAACAGGGC GGGACCCGAG GCCGAGAAGA GGCTGCGCAG AGGGCCAGAG
    GAGGACGGTC CTTGGGGGGC CTGGGACCGC CGAGGGGCCC GCAGCCAGGG CAGAGGTCGC
    CGGGCGCGGC CCACCTCGCC TGAGCTCGAG TCTTCGGATG ACTCCTATGT GTCTGCTGGA
    GAAGAGCCTC TGGAGGCCCC TGTGTTTGAG ATCCCCCTGC AAAATGCAGT GGTGGCCCCA
    GGGGTGGACG TGCTGCTCAA GTGTATTGTT ACCGCCAACC CCCCGCCCCA AGTGTCCTGG
    CAGAAGGATG GGTCCCCGCT GCGCAGTGAT GGCCGCCTTC TCATCCGGGC AGAAGGCGAG
    CGGCACACCC TGCTGCTCCG GGAGGCCCGG GCCGCCGATG CCGGGAGCTA TGCAGCCACT
    GCCACCAACG AGCTGGGCCA GGCCCGCTGC GCTGCCACGC TGGCCGTGAG ACCTGGTGGG
    TCAACATCTC CTTTCAGCAG TCCTATCACC TCTGACGAGG AGTATCTGAG CCCCCCAGAG
    GAGTTCCCGG AACCCGGGGA GACCTGGCCC CGAGCCCCCA CCATGAAGCT CAGTCCTGGC
    CAGAATCGCC GTTCCTCCGA CACTGGCTCC AAGGCGCCCC CTACTTTCAA GGTTTCACTT
    ATGGACCAGT CAGTAAGGGA AGGCCAAGAC GTCACCATGA GCATCCGTGT GCAGGGCGAA
    CCCAAGCCGG TGGTCTCCTG GCTGAGAAAC CGTCAGCCTG TGCGCCCAGA CCAGCGGCGC
    TTTGCTGAGG AGGCAGAGGA TGGGCTCTGC CGGCTTCGGA TCCTGGCTGC CGAGCGGGGC
    GACGCTGGCT TCTACACCTG CAAGGCGGTT AACGAGTATG GCGCTCGACA GTGTGAGGCG
    CGCCTGGAGG TCAGAGCACA CCCTGAAAGC CGGTCCCTGG CCGTGCTGGC CCCTCTGCAG
    GACGTGGACG TGGGGGCCGG GGAGATGGCG CTGTTTGAGT GCCTGGTGGC GGGTCCCACC
    GACGTGGAGG TAGACTGGCT GTGCCGAGGC CGCCTGCTGC AGCCTGCACT ACTCAAATGC
    AAGATGCATT TCGATGGCCG CAAATGCAAG CTGCTGCTCA CCTCTGTGCA TGAGGACGAC
    AGCGGCGTCT ACACCTGCAA GCTCAGCACG GCCAAAGATG AACTGACCTG CAGTGCCCGG
    CTGACCGTGC GACCCTCGCT GGCACCCCTG TTCACGCGGC TGCTGGAGGA CGTGGAAGTG
    CTGGAGGGCC GCGCTGCCCG CTTCGACTGC AAGATCAGCG GGAGCCCGCC CCCCTCCGTT
    ACCTGGACTC ACTTTGGCCA CCCCGTGGAG GAGAGCGAGA ACGTGCGGCT GCGGCAGGAC
    GGGGGCCTGC ATTCGCTGCA CATCGCACAT GTGGGCAGTG AGGATGAGGG GCTGTACGCG
    GTCAGTGCTG CCAACACCCA CGGCCAGGCC CACTGCTCAG CCCAGCTCTA CGTGGAGGAG
    CCTCGGACGG CTGCCACCGG CCCTAGCTCC AAGCTGGAGA AGATGCCCTC CATCCCCGAG
    GAGCCCGAGC AGGGTGAGCT GGAGCGGCTG TCCATGCCCG ACTTCCTGCG GCCGCTGCAG
    GATCTGGACG TGGGACTGGC CAAGGAGGCC ATGCTGGAGT GCCAGGTGAC CGGCCTGCCC
    TACCCCACCA TCAGCTGGTT CCACAACGGC CACCGCATCC AGAGCAGCGA CGACCGACGC
    ATGACGCAGT ACAGGGATGT CCATCGCTTG GTGTTCCCTG CCGTGGGACC TCAGCATGCT
    GGTGTCTACA AGAGCGTCAT TGCTAACAAG CTGGGCAAAG CCGCCTGCTA TGCTCATCTC
    TACGTCACAG ATGTGGTTCC AGGCCCCCCA GACGGTGCCC CTCAGGTAGT GGCTGTGACC
    GGGAGGATGA TCACGCTTGC ATGGAACCCC CCGAGGAGTC TGGACATGGC TATCGACCCA
    GACGCCCTGA CCTACACCGT GCAGCACCAG GTGCTGGGCT CAGACCAGTG GACGGCGCTG
    GTCACGGGCC TGCGGCAGCC CGGGTGGGCA GCCACCGGAT TGCGAAAGGG GGTCCAGCAC
    GTCTTCCGGG TTCTCAGCAC CACCATCAAG AGCAGCAGCA AGCCCTCGCC CCCGTCCGAG
    CCCACGCAGC TGCTGGAGCG TGGCCCGCCC CTGGAGGAGG CGCCCGCTGT GCTGGACAAG
    CCAGACGTCG TGTATGTGGT GGAGGGCCAG CCCACCAGTG TCACCGTCAC CTTCAACCAC
    GTGGAGGCCC AGGTTGTCTG GAGGAGCTGC CGAGGAGCCC TCCTAGAGGC CCGGCCAGGT
    GTGTTTGAGC TGAGCCAGCC AGATGACGAC CAGTACTCTC TTAGGATCTG CCGGGTGAGC
    CGCCGGGACC TGGGAGCCCT CACTTGCACT GCCCGCAACC GCCACGGCAC ACAGGCCTGC
    TCAGTCACCC TGGAGCTGGC AGAGGCCCCT CGGTTTGAGT CCATCATGGA GGATGTGGAG
    GTAGGGGCTG GAGAGACTGC TCGCTTCGCC GTGGTGGTGG ACGGGAAGCC CTTGCCGGAC
    ATCATGTGGT ACAAGGATGA GGCGCTGCTG ACCGAGAGCA ACCATGTGAG CTTCGTGTAC
    GAGGAGAACG AGTGCTCCCT GGTGGTGCTC AGCGCGGGGG CCCAGGATGG AGGGGTCTAC
    ACCTGCACGG CCCGTAACCT GGCTGGCGAA GTCTCCTGCA AAGCAGAGCT GGCTGTGCAC
    TCAGCTCAGA CAGCTATGGA GGTGGAAGGG ATCGGGGAAG ACGAGGAGCA GCGAGGAAGG
    AGGCTCAGCG ACTTCTATGA TATTCAGCAG GAGATCGGCA GGGGTGCCTT CTCCTACCTG
    CGCCGTGTAG TGGAGAGGAG CTCCGGACTG GAGTTTGCAG CCAAGTTCAT CCCCAGCCAG
    GCAAAGCCAA AGGCATCGGC ATGGCGGGAG GCACGGCTGC TGGCCCGGCT CCAGCATGAC
    TGCATCCTCT ACTTCCATGA GGCCTTCGAG AGGCGCCGGG GACTGGTCAT CGTCACCGAG
    CTCTGCACAG AAGAGCTTCT GGAGCGAATG GCCAGGAAAC CCACCGTGTG TGAGTCTGAG
    ATCCGGGCCT ACATGCGGCA GGTACTAGAA GGAATATGCT ACTTGCACGA GAACCACGTG
    CTGCACCTGG ATGTCAAGCC CGAGAACCTG CTGGTGTGGG ATGGTGTTGA GGGCGAGGAG
    CAGGTGAGGA TCTGTGACTT TGGGAACGCT CAAGAGCTGA CACCGGGAGA GCCCCAGTAT
    TGCCAATACG GCACACCTGA GTTCGTGGCG CCCGAGATTG TCAACCAGAC CCCTGTGTCT
    GGAGTCACTG ACATCTGGCC CGTGGGTGTC GTCGCCTTCC TCTGCCTGAC CGGAATCTCC
    CCGTTCGTTG GGGAAAATGA TCGGACGACA TTGATGAACA TCCGAAACTA CAACGTGGCC
    TTCGAGGAGA CCACGTTCCT GAGCCTGAGC AGGGAGGCCC GGGGCTTCCT CATCAAAGTG
    CTGGTGCGGG ACCGGCTGAG GCCTACTGCA GAGGAGACCC TGGAACATCC TTGGTTTAAA
    GCACAGGCAA AGGGTGCAGT GGTGAGCACG GATCACCTAA AGCTATTCCT CTCCCGGCGG
    AGGTGGCAGC GCTCCCAGAT CAGCTACAAG TGCCACCTGG TGCTGCGCCC CATCCCGGAG
    CTGCTGCGAG CACCCCCGGA GCGGGTGTGG GTGGCCGTGC CCCGAAGACC ACCCCCTAGT
    GGAGGGCTGT CGTCATCATC CGACTCTGAA GAGGAAGAGC TGGAGGAGCT GCCCTCTGTG
    CCCCGACCTC TGCAGCCTGA GTTTTCGGGC TCCCGGGTGT CCCTCACTGA CATTCCCACT
    GAGGATGAGG CCCTCGGGGC CCTGGAGGCT GGGGCGGCCA CCCCCATGGA CTGGCAAGAA
    CAAGGAAAGG CCCCCTCTCA GGACCAGGAG GCCCCCAGCC CTCAGGCACT CCCCTCCCCA
    GGCCAGGAGT CCCCGTCCGG GCCCAGCCCC CGGCGGGCAG AGCTCCGCAG GGGCAGCTCG
    GCTGAGAGCG CCCTGCCCCG GGCCGGGCCG AGGGAACCGG GCCGGGGCCT GCACAAGGCA
    GCGTCCGTCG AGCTGCCGCA GCGCCGAAGC CCCAGCCCGG GGGCCACCCG CCTGACCCGA
    GGAGGCCTGG GTGAGGGCGA GTATGCCCAG AGGCTGCAGG CCCTGCGCCA GCGGCTGCTG
    AGGGGTGGCC CCGATGATGG CAAGGTCAGC GGCCTCAGGG GCCCCCTGCT GGAGAGCCTG
    GGGGGCCGGG CTCGTGACCC CCGGCTGGCG CGGGCAGCCT CCAGCGAGGC AGCACCCCAT
    CACCAGCACC CACCTGAGGC TCGGGGCCTG CAGAAGAGCA GTAGCTTCTC GCAGGGTGAG
    GCAGAGCCCC GGGGCCGGCA CCGCCGAGCA GGGGCACCCC TTGAGATCCC CGTGGCCCGG
    CTGGGGGCCC GCAAGCTGCA GGAGTCTCCC TCCTTGTCCG CCCTCAGCGA GGCCCAACCA
    CCTAGCCCCG TGCGGCCCAG TGCAGCCAAG CCCAGCACCC CTAAGTCTTC AGAACCTCTT
    GTGGGCACCC CCAGTGAGGC TGCACAGCCT CCAGCACCCC AGCCTGCCCG AGAGAAGGCC
    CCAGAATCTA CGCCAGAACC AGCTCGGGGC CCCAAGCCCA CACAGCCCCC CACAGCTCTG
    CAAACCCTAG CGCCTCCTCT CACTCCGTAT GCGCAGATCA TGCAGTCGCT CCAGCTGCCA
    GGCCAGGCGC AAGGCCCCCC ACAGGGCGCA GTCGTATCTC CTACATCTCG GTCTCCAGCA
    TCTCGGTTGG AACCCAAGCC CCACCCGGCT GTGTTCTCCA GGGTGGCCTC CCCACCTCCA
    GGAGCCTCCG AGAAGCGCCT GCTGCCGGGA GCCGGAGCAA CCCCAGGGCC AGCCGAAAAA
    GCCCGGGTCC CGGCGGTCCC CCGCAGGCCA GGGAGCAGTC TCAGCAGCAG CATCGAGAAC
    CTGGAGTCGG AGGCCGTGTT CGAGGCCAAG TTCAAGCGCA GCCGGGAGTC GCCCCTGTCG
    CGGGGGCTGC GGCTGCTGAG CCGCTCGCGG TCGGAGGAGC GCGGCCCCTT CCGCGGGGCT
    GAGGAGGAGG ACGGCATTTA CCGGCCCAGC CCGGCGGGCA CGCCGCTGGA GCTGGTGCGA
    CGGCCCGAGC GCTCGCGCTC CGTGCAGGAC CTCAGGGCGG TCGGGGAGCC AGGCCTCGTG
    CGCCGCCTCT CGCTGTCGCT GTCCCAGCGT CTGCGGCGGA CCCCGCCCGC GCAGCGCCAC
    CCGGCCTGGG AGCCCCGCGG CGGGGACGGC GAGAGCTCGG AGGGCGGCAG CTCGGCGCGG
    GGCTCCCCGG TGCTGACGGT GCGCCGGAGG CTCAGCTCCA CGCTGGAGCG GCTGTCGAGC
    CGGTTGCAGC GCAGCGGCAG CAGCGAGGAC TCGGGGGGCG CGTCGGGCCG CAGCACGCCG
    CTGTTCGGCC GGCTGCGCAG GGCCACGTCC GAGAGCGAGA GTCTGCGGCG CCTGGACCTC
    GCGCAAAATC AGCTGGCGGC CCAAGCCGGC GCCGCCACCC CTTCCGCGGA GTCCCTGGGC
    TCCGAGGCCA GCGCTACGTC GGGCTCCTCT GCTCCTGCAG AAAGTCGAAG CCGCCTGCGC
    TGGGGCCTCT CTCGGCTGCG GAAGGACAAG AGGTTGTCAC AGCCTAACCT CTCTGGCAGT
    GTCCAGGAGG ATTTGGGTCA CCAGTATGTG CGCAGTGAGT CAGACTTTCC CCCAGTCTTC
    CACATCAAAC TCAAGGACCA GGTGCTGCTG GAGGGAGAGG CAGCCACCCT GCTCTGCTTG
    CCGGCAGCCT GCCCTGCACC CCACATCTCC TGGATGAAAG ACAAGCAGTC CCTGCGGTCA
    GAGCCCTCGG TGATCATCGT GTCCTGCAAA GATGGTCGTC AGCTACTGAG CATCCCCCGG
    GCAGGCAAGC GGCATGCGGG GCTCTATGAG TGCTCCGCCA CCAACGTCCT TGGCAGCATC
    ACCAGCTCCT GTACCGTGGC TGTGGCTCGT ACCCCAGGAA AGCTAGCTCC TCCTGAGGTG
    CCCCAGACCT ACCAGGACAC AGCACTGGTG CTCTGGAAGC CGGGAGACAG CAAGGCGCCT
    TGCACATACA CGCTGGAGCG GCGGGTGGAT GGGGAGTCTT CCTGGCACCC TGTGAGCTCG
    GGCATCCCCG ACTGCTACTA CAACGTGACC CACCTACCAG TCGGCATGGC TGTGAGGTTC
    CGTGTGGCCT GTGCCAACCG TGCTGGGCAA GGGCCTTTCA GCAACCCTTC TGAGAAGGTC
    CTCGTCAGGG GCGTGCAAGA TTCCTCAGCT CTGCCATCTG CTGCTCACAG AGATGCCCCT
    GTTACCTCAG GGCCAGCCAG GGCCCCGCCT CCTGACGCTC CTACCTCACT GGCCCCTCCC
    CTGAGCCCCT CCCCAGCTCC TGCTCCCCCA GGCCCCCAGG CAGCCCCTCT CGGCCCTTCA
    TCTTCCCCAG CACCCCCCAG CCAGGCCTTG TCCTCCCTCA AGGCTGTGGG TCCACCACCC
    CAAACCCCCC CACGAAAGCA CCGGGGCCTG CAGGCTGCCC AGCAAGTAGA ACCTACCCCA
    CCCAGTGCCC AGGACTCCCC AAGTGAGCCC AAGTCTTCCG TCCCTGACAC TGGGACCCCC
    ATCCCAGCCT CTGCCCCTCA AGTGGTGAAA ACAGCCTCTT CCTCAATGCC CCTGTATATG
    GTGACCTCCT TTGTGTCTGC ACCACCAGCC CCTGAGCCCC CAGCCCCCGA GCCCCCTCCT
    GAGCCCACCA AAGTGACTGT GCGGAGCCTC AGCCCAGCCA AGGAGGTGGT TGGCTCCCCT
    GGGGGTGGTC CCCCCAGGGC TCCTAAGCCA GAGGGCACCA CTCTTCGACA GGGCCCTCCT
    CAGAAACCAT ACACCTTCCT GGAGGAGAAG GCCAGGGGTC GCTTTGGTGT GGTGCGTGCC
    TGCCGGGAGA ATGCCACCGG GCGAACATTC GTGGCCAAGA TTGTGCCATA CGCGGCCGAG
    GGCAAGCGGC AGGTCCTGCA GGAGTACGAG GTGCTGAGGA CCCTGCACCA CGAGCGGCTT
    ATGGCCCTGC ACGAAGCCTA CATCACCCCG CGCTACCTCG TGCTCATCGC CGAGAGCTGC
    GGCAACCGGG AGCTTCTCTG TGGGCTCAGC GACAGATTCC GGTATTCAGA GGATGATGTG
    GCCACCTATG TGGTGCAGCT GCTACAAGGC CTGGATTACC TCCATGGCCG CCACGTGCTG
    CACCTGGACA TCAAGCCAGA CAACCTGCTG CTGGCCCCTG ACAACGCCCT CAAGATCGTG
    GACTTTGGCA GTGCCCAGCC CTACAACCCC CAGGCTCTGC GGCCCCTGGG TCACCGCACG
    GGCACACTGG AGTTCATGGC TCCTGAGATG GTAAAGGGAG ACCCCATCGG CTCTGCCACG
    GACATCTGGG GAGCGGGTGT GCTCACTTAC ATCATGCTCA GTGGACGCTC CCCATTCTAT
    GAGCCAGACC CCCAGGAAAC AGAGGCTCGC ATTGTGGGGG GCCGCTTTGA TGCCTTCCAG
    CTGTATCCCA ACACATCCCA GAGTGCCACC CTCTTCTTGC GAAAGGTCCT CTCAGTACAT
    CCCTGGAGCC GGCCCTCCGT GCAGGACTGC CTGGCTCACC CGTGGCTGCA GGATGCCTAC
    CTGATGAAAC TGCGCCGCCA GACACTCACC TTCACCACCA ACCGGCTCAA GGAGTTCCTG
    GGTGAGCAGC GGCGCCGCAG GGCCGAGGCC GCCACCCGCC ACAAGGTGCT GCTCCGCTCT
    TACCCAGGCA GCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87987
    Clone ID Related Accession (Same CDS sequence) XM_015459155.1
    Accession Version XM_015459155.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product striated muscle preferentially expressed protein kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103849.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCGGGCA CGAGGGCACC CCCCAGCCCC 
    GGGGTGCCCC CGAAGAGGGC CAAAGTGGGG GCCGGCGGAG GGGCGGCGGC CGCGGTGGCC
    GGGGCGCCGG TCTTCCTACG GCCCCTGAAG GACGCGGCGG TGTCTGCGGG CAGCGACGTG
    CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCAGCTGGTT CCGGGATGGG
    CAGCGCTTGC CCCCGCCGGC CCCTGAGCCC AGCTGCCTGT GGCTGCGAAG CTGCGGGCCG
    CGGGATGCCG GCGTGTACAG CTGCAGGGCC CAGAACGAGC TGGGCCAGGC CTCCTGCGAG
    GCGGTTCTCA CCGTGCTGGA GGTCGGAGAC TCGGAGACAG GCGAGGATGA CATCAGCGAC
    GGGCAGGGGA CCCAGCGCCT GGAGCTTCGG GATGATGGGG CCTTCAGCAC CCCCACGGGG
    GGCTCTGATA CCCTGGTGGG CACCTCCCTG GACACGCCCC CGACCTCCGT GACAGGCACC
    TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC
    AGCAGGGCTG GCACCCGCCG CCTCCCGGGC AGCCCAAGCA GTGTCCCTCA GAGCGGACTG
    CACAAGGAGG AGCCTGACCT TCAGCCTCAG CCCGCCAGCG AAGCCCCCCG TCGCCCAGCC
    CTGCCGCCCC CCTCCAAATC CGCGCTGCTG CCCCCACCGT CCCCTCGGGT GGGTAAGCGG
    CCCCCGCCGG GAGCCGGCGG CCAGCCCCTG GCCACCCCCA CGCCGCCGCA CCGGCGCTCT
    CGGGAGCCGG TGCCGCCCGA GGACGCCACC GCCGAAGAAA AGCGAGGGAA AAAGTCCAAG
    TCGTCCGGGC CCTCGCTAGC GGGCACGGCG GAGTCCCGGC CCCAGACGCC CCTGAGTGAG
    GCCTCGGGCC GCCAGTCGGC GCTGGGCCGC TCCCCGAGGC TGGTGCGCGC TGGCTCCCGC
    ATCCTGGACA AGCTGCAGTT CTTCGAGGAG CGCCGGCGCA GCCTGGAGCG CAGCGACTCG
    CCGCCGGCGC CCCTGCGGCC CTGGGTGCCC CTGCGCAAGG CCCGTTCGCT GGAGCAGCCC
    AAGTCCGAGG GCGGCGGGCC GTGGGGCACG CCCAGGGCCT CGCAGGAGGA GCTGCGGTCC
    CCGGCGGGCA GCGTGGCCGA GCGGCGCCGC CTATTCCAAC AGAAGGCGGC CTCGCTGGAC
    GAGCGCACGC GTCAGCGCAG CCCTGCGTCC GACCTCGAGC TGCGCTTCGC CCAGGAGCTG
    GGCCGCATCC GCCGCTCCAC GTCGCGGGAG GAGCTGGTGC GCTCTCACGA GTCCCTGCGC
    GCCACGCTGC AGCGCGCCCC GTCCCCCCGA GAGCCCGGCG AGCCGCCGCT CTTCTCCCAG
    CCCTCCACCC CCAAGACCCC GCGGGCCTTG AGCCCCGCCG CCGCCCAGCC GCCCCCTGCG
    AGCGTCGCGG AAAAGCCCGG GGATGAGCCA GGGAGGCCCC GGGGCCGTGG GCCAGCGGGC
    AGGACGGAGC CGGGGGAAGG CCCGCAGCAG GAGGTTAGGC GCCGGGACCA GTTCCCGCTG
    ACCCGGAGCA GAGCCATCCA GGAGTGCCGG AGCCCGGTGC CGCCCCCCAC CTCTGAGCCC
    CCCGAGACCA GGACGAAAGC TCCCCCGGGT CGGAAGCGGG AGCCCCCGCC GCAGGCCGTG
    CGCTTCCTGC CCTGGGCCAC GCCGGGCCAG GAGGGCGCTG CTGTGCCCCA GACCTTGGAG
    AAGAACAGGG CGGGACCCGA GGCCGAGAAG AGGCTGCGCA GAGGGCCAGA GGAGGACGGT
    CCTTGGGGGG CCTGGGACCG CCGAGGGGCC CGCAGCCAGG GCAGAGGTCG CCGGGCGCGG
    CCCACCTCGC CTGAGCTCGA GTCTTCGGAT GACTCCTATG TGTCTGCTGG AGAAGAGCCT
    CTGGAGGCCC CTGTGTTTGA GATCCCCCTG CAAAATGCAG TGGTGGCCCC AGGGGTGGAC
    GTGCTGCTCA AGTGTATTGT TACCGCCAAC CCCCCGCCCC AAGTGTCCTG GCAGAAGGAT
    GGGTCCCCGC TGCGCAGTGA TGGCCGCCTT CTCATCCGGG CAGAAGGCGA GCGGCACACC
    CTGCTGCTCC GGGAGGCCCG GGCCGCCGAT GCCGGGAGCT ATGCAGCCAC TGCCACCAAC
    GAGCTGGGCC AGGCCCGCTG CGCTGCCACG CTGGCCGTGA GACCTGGTGG GTCAACATCT
    CCTTTCAGCA GTCCTATCAC CTCTGACGAG GAGTATCTGA GCCCCCCAGA GGAGTTCCCG
    GAACCCGGGG AGACCTGGCC CCGAGCCCCC ACCATGAAGC TCAGTCCTGG CCAGAATCGC
    CGTTCCTCCG ACACTGGCTC CAAGGCGCCC CCTACTTTCA AGGTTTCACT TATGGACCAG
    TCAGTAAGGG AAGGCCAAGA CGTCACCATG AGCATCCGTG TGCAGGGCGA ACCCAAGCCG
    GTGGTCTCCT GGCTGAGAAA CCGTCAGCCT GTGCGCCCAG ACCAGCGGCG CTTTGCTGAG
    GAGGCAGAGG ATGGGCTCTG CCGGCTTCGG ATCCTGGCTG CCGAGCGGGG CGACGCTGGC
    TTCTACACCT GCAAGGCGGT TAACGAGTAT GGCGCTCGAC AGTGTGAGGC GCGCCTGGAG
    GTCAGAGCAC ACCCTGAAAG CCGGTCCCTG GCCGTGCTGG CCCCTCTGCA GGACGTGGAC
    GTGGGGGCCG GGGAGATGGC GCTGTTTGAG TGCCTGGTGG CGGGTCCCAC CGACGTGGAG
    GTAGACTGGC TGTGCCGAGG CCGCCTGCTG CAGCCTGCAC TACTCAAATG CAAGATGCAT
    TTCGATGGCC GCAAATGCAA GCTGCTGCTC ACCTCTGTGC ATGAGGACGA CAGCGGCGTC
    TACACCTGCA AGCTCAGCAC GGCCAAAGAT GAACTGACCT GCAGTGCCCG GCTGACCGTG
    CGACCCTCGC TGGCACCCCT GTTCACGCGG CTGCTGGAGG ACGTGGAAGT GCTGGAGGGC
    CGCGCTGCCC GCTTCGACTG CAAGATCAGC GGGAGCCCGC CCCCCTCCGT TACCTGGACT
    CACTTTGGCC ACCCCGTGGA GGAGAGCGAG AACGTGCGGC TGCGGCAGGA CGGGGGCCTG
    CATTCGCTGC ACATCGCACA TGTGGGCAGT GAGGATGAGG GGCTGTACGC GGTCAGTGCT
    GCCAACACCC ACGGCCAGGC CCACTGCTCA GCCCAGCTCT ACGTGGAGGA GCCTCGGACG
    GCTGCCACCG GCCCTAGCTC CAAGCTGGAG AAGATGCCCT CCATCCCCGA GGAGCCCGAG
    CAGGGTGAGC TGGAGCGGCT GTCCATGCCC GACTTCCTGC GGCCGCTGCA GGATCTGGAC
    GTGGGACTGG CCAAGGAGGC CATGCTGGAG TGCCAGGTGA CCGGCCTGCC CTACCCCACC
    ATCAGCTGGT TCCACAACGG CCACCGCATC CAGAGCAGCG ACGACCGACG CATGACGCAG
    TACAGGGATG TCCATCGCTT GGTGTTCCCT GCCGTGGGAC CTCAGCATGC TGGTGTCTAC
    AAGAGCGTCA TTGCTAACAA GCTGGGCAAA GCCGCCTGCT ATGCTCATCT CTACGTCACA
    GATGTGGTTC CAGGCCCCCC AGACGGTGCC CCTCAGGTAG TGGCTGTGAC CGGGAGGATG
    ATCACGCTTG CATGGAACCC CCCGAGGAGT CTGGACATGG CTATCGACCC AGACGCCCTG
    ACCTACACCG TGCAGCACCA GGTGCTGGGC TCAGACCAGT GGACGGCGCT GGTCACGGGC
    CTGCGGCAGC CCGGGTGGGC AGCCACCGGA TTGCGAAAGG GGGTCCAGCA CGTCTTCCGG
    GTTCTCAGCA CCACCATCAA GAGCAGCAGC AAGCCCTCGC CCCCGTCCGA GCCCACGCAG
    CTGCTGGAGC GTGGCCCGCC CCTGGAGGAG GCGCCCGCTG TGCTGGACAA GCCAGACGTC
    GTGTATGTGG TGGAGGGCCA GCCCACCAGT GTCACCGTCA CCTTCAACCA CGTGGAGGCC
    CAGGTTGTCT GGAGGAGCTG CCGAGGAGCC CTCCTAGAGG CCCGGCCAGG TGTGTTTGAG
    CTGAGCCAGC CAGATGACGA CCAGTACTCT CTTAGGATCT GCCGGGTGAG CCGCCGGGAC
    CTGGGAGCCC TCACTTGCAC TGCCCGCAAC CGCCACGGCA CACAGGCCTG CTCAGTCACC
    CTGGAGCTGG CAGAGGCCCC TCGGTTTGAG TCCATCATGG AGGATGTGGA GGTAGGGGCT
    GGAGAGACTG CTCGCTTCGC CGTGGTGGTG GACGGGAAGC CCTTGCCGGA CATCATGTGG
    TACAAGGATG AGGCGCTGCT GACCGAGAGC AACCATGTGA GCTTCGTGTA CGAGGAGAAC
    GAGTGCTCCC TGGTGGTGCT CAGCGCGGGG GCCCAGGATG GAGGGGTCTA CACCTGCACG
    GCCCGTAACC TGGCTGGCGA AGTCTCCTGC AAAGCAGAGC TGGCTGTGCA CTCAGCTCAG
    ACAGCTATGG AGGTGGAAGG GATCGGGGAA GACGAGGAGC AGCGAGGAAG GAGGCTCAGC
    GACTTCTATG ATATTCAGCA GGAGATCGGC AGGGGTGCCT TCTCCTACCT GCGCCGTGTA
    GTGGAGAGGA GCTCCGGACT GGAGTTTGCA GCCAAGTTCA TCCCCAGCCA GGCAAAGCCA
    AAGGCATCGG CATGGCGGGA GGCACGGCTG CTGGCCCGGC TCCAGCATGA CTGCATCCTC
    TACTTCCATG AGGCCTTCGA GAGGCGCCGG GGACTGGTCA TCGTCACCGA GCTCTGCACA
    GAAGAGCTTC TGGAGCGAAT GGCCAGGAAA CCCACCGTGT GTGAGTCTGA GATCCGGGCC
    TACATGCGGC AGGTACTAGA AGGAATATGC TACTTGCACG AGAACCACGT GCTGCACCTG
    GATGTCAAGC CCGAGAACCT GCTGGTGTGG GATGGTGTTG AGGGCGAGGA GCAGGTGAGG
    ATCTGTGACT TTGGGAACGC TCAAGAGCTG ACACCGGGAG AGCCCCAGTA TTGCCAATAC
    GGCACACCTG AGTTCGTGGC GCCCGAGATT GTCAACCAGA CCCCTGTGTC TGGAGTCACT
    GACATCTGGC CCGTGGGTGT CGTCGCCTTC CTCTGCCTGA CCGGAATCTC CCCGTTCGTT
    GGGGAAAATG ATCGGACGAC ATTGATGAAC ATCCGAAACT ACAACGTGGC CTTCGAGGAG
    ACCACGTTCC TGAGCCTGAG CAGGGAGGCC CGGGGCTTCC TCATCAAAGT GCTGGTGCGG
    GACCGGCTGA GGCCTACTGC AGAGGAGACC CTGGAACATC CTTGGTTTAA AGCACAGGCA
    AAGGGTGCAG TGGTGAGCAC GGATCACCTA AAGCTATTCC TCTCCCGGCG GAGGTGGCAG
    CGCTCCCAGA TCAGCTACAA GTGCCACCTG GTGCTGCGCC CCATCCCGGA GCTGCTGCGA
    GCACCCCCGG AGCGGGTGTG GGTGGCCGTG CCCCGAAGAC CACCCCCTAG TGGAGGGCTG
    TCGTCATCAT CCGACTCTGA AGAGGAAGAG CTGGAGGAGC TGCCCTCTGT GCCCCGACCT
    CTGCAGCCTG AGTTTTCGGG CTCCCGGGTG TCCCTCACTG ACATTCCCAC TGAGGATGAG
    GCCCTCGGGG CCCTGGAGGC TGGGGCGGCC ACCCCCATGG ACTGGCAAGA ACAAGGAAAG
    GCCCCCTCTC AGGACCAGGA GGCCCCCAGC CCTCAGGCAC TCCCCTCCCC AGGCCAGGAG
    TCCCCGTCCG GGCCCAGCCC CCGGCGGGCA GAGCTCCGCA GGGGCAGCTC GGCTGAGAGC
    GCCCTGCCCC GGGCCGGGCC GAGGGAACCG GGCCGGGGCC TGCACAAGGC AGCGTCCGTC
    GAGCTGCCGC AGCGCCGAAG CCCCAGCCCG GGGGCCACCC GCCTGACCCG AGGAGGCCTG
    GGTGAGGGCG AGTATGCCCA GAGGCTGCAG GCCCTGCGCC AGCGGCTGCT GAGGGGTGGC
    CCCGATGATG GCAAGGTCAG CGGCCTCAGG GGCCCCCTGC TGGAGAGCCT GGGGGGCCGG
    GCTCGTGACC CCCGGCTGGC GCGGGCAGCC TCCAGCGAGG CAGCACCCCA TCACCAGCAC
    CCACCTGAGG CTCGGGGCCT GCAGAAGAGC AGTAGCTTCT CGCAGGGTGA GGCAGAGCCC
    CGGGGCCGGC ACCGCCGAGC AGGGGCACCC CTTGAGATCC CCGTGGCCCG GCTGGGGGCC
    CGCAAGCTGC AGGAGTCTCC CTCCTTGTCC GCCCTCAGCG AGGCCCAACC ACCTAGCCCC
    GTGCGGCCCA GTGCAGCCAA GCCCAGCACC CCTAAGTCTT CAGAACCTCT TGTGGGCACC
    CCCAGTGAGG CTGCACAGCC TCCAGCACCC CAGCCTGCCC GAGAGAAGGC CCCAGAATCT
    ACGCCAGAAC CAGCTCGGGG CCCCAAGCCC ACACAGCCCC CCACAGCTCT GCAAACCCTA
    GCGCCTCCTC TCACTCCGTA TGCGCAGATC ATGCAGTCGC TCCAGCTGCC AGGCCAGGCG
    CAAGGCCCCC CACAGGGCGC AGTCGTATCT CCTACATCTC GGTCTCCAGC ATCTCGGTTG
    GAACCCAAGC CCCACCCGGC TGTGTTCTCC AGGGTGGCCT CCCCACCTCC AGGAGCCTCC
    GAGAAGCGCC TGCTGCCGGG AGCCGGAGCA ACCCCAGGGC CAGCCGAAAA AGCCCGGGTC
    CCGGCGGTCC CCCGCAGGCC AGGGAGCAGT CTCAGCAGCA GCATCGAGAA CCTGGAGTCG
    GAGGCCGTGT TCGAGGCCAA GTTCAAGCGC AGCCGGGAGT CGCCCCTGTC GCGGGGGCTG
    CGGCTGCTGA GCCGCTCGCG GTCGGAGGAG CGCGGCCCCT TCCGCGGGGC TGAGGAGGAG
    GACGGCATTT ACCGGCCCAG CCCGGCGGGC ACGCCGCTGG AGCTGGTGCG ACGGCCCGAG
    CGCTCGCGCT CCGTGCAGGA CCTCAGGGCG GTCGGGGAGC CAGGCCTCGT GCGCCGCCTC
    TCGCTGTCGC TGTCCCAGCG TCTGCGGCGG ACCCCGCCCG CGCAGCGCCA CCCGGCCTGG
    GAGCCCCGCG GCGGGGACGG CGAGAGCTCG GAGGGCGGCA GCTCGGCGCG GGGCTCCCCG
    GTGCTGACGG TGCGCCGGAG GCTCAGCTCC ACGCTGGAGC GGCTGTCGAG CCGGTTGCAG
    CGCAGCGGCA GCAGCGAGGA CTCGGGGGGC GCGTCGGGCC GCAGCACGCC GCTGTTCGGC
    CGGCTGCGCA GGGCCACGTC CGAGAGCGAG AGTCTGCGGC GCCTGGACCT CGCGCAAAAT
    CAGCTGGCGG CCCAAGCCGG CGCCGCCACC CCTTCCGCGG AGTCCCTGGG CTCCGAGGCC
    AGCGCTACGT CGGGCTCCTC TGCTCCTGCA GAAAGTCGAA GCCGCCTGCG CTGGGGCCTC
    TCTCGGCTGC GGAAGGACAA GAGGTTGTCA CAGCCTAACC TCTCTGGCAG TGTCCAGGAG
    GATTTGGGTC ACCAGTATGT GCGCAGTGAG TCAGACTTTC CCCCAGTCTT CCACATCAAA
    CTCAAGGACC AGGTGCTGCT GGAGGGAGAG GCAGCCACCC TGCTCTGCTT GCCGGCAGCC
    TGCCCTGCAC CCCACATCTC CTGGATGAAA GACAAGCAGT CCCTGCGGTC AGAGCCCTCG
    GTGATCATCG TGTCCTGCAA AGATGGTCGT CAGCTACTGA GCATCCCCCG GGCAGGCAAG
    CGGCATGCGG GGCTCTATGA GTGCTCCGCC ACCAACGTCC TTGGCAGCAT CACCAGCTCC
    TGTACCGTGG CTGTGGCTCG TACCCCAGGA AAGCTAGCTC CTCCTGAGGT GCCCCAGACC
    TACCAGGACA CAGCACTGGT GCTCTGGAAG CCGGGAGACA GCAAGGCGCC TTGCACATAC
    ACGCTGGAGC GGCGGGTGGA TGGGGAGTCT TCCTGGCACC CTGTGAGCTC GGGCATCCCC
    GACTGCTACT ACAACGTGAC CCACCTACCA GTCGGCATGG CTGTGAGGTT CCGTGTGGCC
    TGTGCCAACC GTGCTGGGCA AGGGCCTTTC AGCAACCCTT CTGAGAAGGT CCTCGTCAGG
    GGCGTGCAAG ATTCCTCAGC TCTGCCATCT GCTGCTCACA GAGATGCCCC TGTTACCTCA
    GGGCCAGCCA GGGCCCCGCC TCCTGACGCT CCTACCTCAC TGGCCCCTCC CCTGAGCCCC
    TCCCCAGCTC CTGCTCCCCC AGGCCCCCAG GCAGCCCCTC TCGGCCCTTC ATCTTCCCCA
    GCACCCCCCA GCCAGGCCTT GTCCTCCCTC AAGGCTGTGG GTCCACCACC CCAAACCCCC
    CCACGAAAGC ACCGGGGCCT GCAGGCTGCC CAGCAAGTAG AACCTACCCC ACCCAGTGCC
    CAGGACTCCC CAAGTGAGCC CAAGTCTTCC GTCCCTGACA CTGGGACCCC CATCCCAGCC
    TCTGCCCCTC AAGTGGTGAA AACAGCCTCT TCCTCAATGC CCCTGTATAT GGTGACCTCC
    TTTGTGTCTG CACCACCAGC CCCTGAGCCC CCAGCCCCCG AGCCCCCTCC TGAGCCCACC
    AAAGTGACTG TGCGGAGCCT CAGCCCAGCC AAGGAGGTGG TTGGCTCCCC TGGGGGTGGT
    CCCCCCAGGG CTCCTAAGCC AGAGGGCACC ACTCTTCGAC AGGGCCCTCC TCAGAAACCA
    TACACCTTCC TGGAGGAGAA GGCCAGGGGT CGCTTTGGTG TGGTGCGTGC CTGCCGGGAG
    AATGCCACCG GGCGAACATT CGTGGCCAAG ATTGTGCCAT ACGCGGCCGA GGGCAAGCGG
    CAGGTCCTGC AGGAGTACGA GGTGCTGAGG ACCCTGCACC ACGAGCGGCT TATGGCCCTG
    CACGAAGCCT ACATCACCCC GCGCTACCTC GTGCTCATCG CCGAGAGCTG CGGCAACCGG
    GAGCTTCTCT GTGGGCTCAG CGACAGATTC CGGTATTCAG AGGATGATGT GGCCACCTAT
    GTGGTGCAGC TGCTACAAGG CCTGGATTAC CTCCATGGCC GCCACGTGCT GCACCTGGAC
    ATCAAGCCAG ACAACCTGCT GCTGGCCCCT GACAACGCCC TCAAGATCGT GGACTTTGGC
    AGTGCCCAGC CCTACAACCC CCAGGCTCTG CGGCCCCTGG GTCACCGCAC GGGCACACTG
    GAGTTCATGG CTCCTGAGAT GGTAAAGGGA GACCCCATCG GCTCTGCCAC GGACATCTGG
    GGAGCGGGTG TGCTCACTTA CATCATGCTC AGTGGACGCT CCCCATTCTA TGAGCCAGAC
    CCCCAGGAAA CAGAGGCTCG CATTGTGGGG GGCCGCTTTG ATGCCTTCCA GCTGTATCCC
    AACACATCCC AGAGTGCCAC CCTCTTCTTG CGAAAGGTCC TCTCAGTACA TCCCTGGAGC
    CGGCCCTCCG TGCAGGACTG CCTGGCTCAC CCGTGGCTGC AGGATGCCTA CCTGATGAAA
    CTGCGCCGCC AGACACTCAC CTTCACCACC AACCGGCTCA AGGAGTTCCT GGGTGAGCAG
    CGGCGCCGCA GGGCCGAGGC CGCCACCCGC CACAAGGTGC TGCTCCGCTC TTACCCAGGC
    AGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314641.1
    CDS237..9905
    Translation

    Target ORF information:

    RefSeq Version XM_015459155.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SPEG complex locus (SPEG), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459155.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCGGGCA CGAGGGCACC CCCCAGCCCC 
    GGGGTGCCCC CGAAGAGGGC CAAAGTGGGG GCCGGCGGAG GGGCGGCGGC CGCGGTGGCC
    GGGGCGCCGG TCTTCCTACG GCCCCTGAAG GACGCGGCGG TGTCTGCGGG CAGCGACGTG
    CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCAGCTGGTT CCGGGATGGG
    CAGCGCTTGC CCCCGCCGGC CCCTGAGCCC AGCTGCCTGT GGCTGCGAAG CTGCGGGCCG
    CGGGATGCCG GCGTGTACAG CTGCAGGGCC CAGAACGAGC TGGGCCAGGC CTCCTGCGAG
    GCGGTTCTCA CCGTGCTGGA GGTCGGAGAC TCGGAGACAG GCGAGGATGA CATCAGCGAC
    GGGCAGGGGA CCCAGCGCCT GGAGCTTCGG GATGATGGGG CCTTCAGCAC CCCCACGGGG
    GGCTCTGATA CCCTGGTGGG CACCTCCCTG GACACGCCCC CGACCTCCGT GACAGGCACC
    TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC
    AGCAGGGCTG GCACCCGCCG CCTCCCGGGC AGCCCAAGCA GTGTCCCTCA GAGCGGACTG
    CACAAGGAGG AGCCTGACCT TCAGCCTCAG CCCGCCAGCG AAGCCCCCCG TCGCCCAGCC
    CTGCCGCCCC CCTCCAAATC CGCGCTGCTG CCCCCACCGT CCCCTCGGGT GGGTAAGCGG
    CCCCCGCCGG GAGCCGGCGG CCAGCCCCTG GCCACCCCCA CGCCGCCGCA CCGGCGCTCT
    CGGGAGCCGG TGCCGCCCGA GGACGCCACC GCCGAAGAAA AGCGAGGGAA AAAGTCCAAG
    TCGTCCGGGC CCTCGCTAGC GGGCACGGCG GAGTCCCGGC CCCAGACGCC CCTGAGTGAG
    GCCTCGGGCC GCCAGTCGGC GCTGGGCCGC TCCCCGAGGC TGGTGCGCGC TGGCTCCCGC
    ATCCTGGACA AGCTGCAGTT CTTCGAGGAG CGCCGGCGCA GCCTGGAGCG CAGCGACTCG
    CCGCCGGCGC CCCTGCGGCC CTGGGTGCCC CTGCGCAAGG CCCGTTCGCT GGAGCAGCCC
    AAGTCCGAGG GCGGCGGGCC GTGGGGCACG CCCAGGGCCT CGCAGGAGGA GCTGCGGTCC
    CCGGCGGGCA GCGTGGCCGA GCGGCGCCGC CTATTCCAAC AGAAGGCGGC CTCGCTGGAC
    GAGCGCACGC GTCAGCGCAG CCCTGCGTCC GACCTCGAGC TGCGCTTCGC CCAGGAGCTG
    GGCCGCATCC GCCGCTCCAC GTCGCGGGAG GAGCTGGTGC GCTCTCACGA GTCCCTGCGC
    GCCACGCTGC AGCGCGCCCC GTCCCCCCGA GAGCCCGGCG AGCCGCCGCT CTTCTCCCAG
    CCCTCCACCC CCAAGACCCC GCGGGCCTTG AGCCCCGCCG CCGCCCAGCC GCCCCCTGCG
    AGCGTCGCGG AAAAGCCCGG GGATGAGCCA GGGAGGCCCC GGGGCCGTGG GCCAGCGGGC
    AGGACGGAGC CGGGGGAAGG CCCGCAGCAG GAGGTTAGGC GCCGGGACCA GTTCCCGCTG
    ACCCGGAGCA GAGCCATCCA GGAGTGCCGG AGCCCGGTGC CGCCCCCCAC CTCTGAGCCC
    CCCGAGACCA GGACGAAAGC TCCCCCGGGT CGGAAGCGGG AGCCCCCGCC GCAGGCCGTG
    CGCTTCCTGC CCTGGGCCAC GCCGGGCCAG GAGGGCGCTG CTGTGCCCCA GACCTTGGAG
    AAGAACAGGG CGGGACCCGA GGCCGAGAAG AGGCTGCGCA GAGGGCCAGA GGAGGACGGT
    CCTTGGGGGG CCTGGGACCG CCGAGGGGCC CGCAGCCAGG GCAGAGGTCG CCGGGCGCGG
    CCCACCTCGC CTGAGCTCGA GTCTTCGGAT GACTCCTATG TGTCTGCTGG AGAAGAGCCT
    CTGGAGGCCC CTGTGTTTGA GATCCCCCTG CAAAATGCAG TGGTGGCCCC AGGGGTGGAC
    GTGCTGCTCA AGTGTATTGT TACCGCCAAC CCCCCGCCCC AAGTGTCCTG GCAGAAGGAT
    GGGTCCCCGC TGCGCAGTGA TGGCCGCCTT CTCATCCGGG CAGAAGGCGA GCGGCACACC
    CTGCTGCTCC GGGAGGCCCG GGCCGCCGAT GCCGGGAGCT ATGCAGCCAC TGCCACCAAC
    GAGCTGGGCC AGGCCCGCTG CGCTGCCACG CTGGCCGTGA GACCTGGTGG GTCAACATCT
    CCTTTCAGCA GTCCTATCAC CTCTGACGAG GAGTATCTGA GCCCCCCAGA GGAGTTCCCG
    GAACCCGGGG AGACCTGGCC CCGAGCCCCC ACCATGAAGC TCAGTCCTGG CCAGAATCGC
    CGTTCCTCCG ACACTGGCTC CAAGGCGCCC CCTACTTTCA AGGTTTCACT TATGGACCAG
    TCAGTAAGGG AAGGCCAAGA CGTCACCATG AGCATCCGTG TGCAGGGCGA ACCCAAGCCG
    GTGGTCTCCT GGCTGAGAAA CCGTCAGCCT GTGCGCCCAG ACCAGCGGCG CTTTGCTGAG
    GAGGCAGAGG ATGGGCTCTG CCGGCTTCGG ATCCTGGCTG CCGAGCGGGG CGACGCTGGC
    TTCTACACCT GCAAGGCGGT TAACGAGTAT GGCGCTCGAC AGTGTGAGGC GCGCCTGGAG
    GTCAGAGCAC ACCCTGAAAG CCGGTCCCTG GCCGTGCTGG CCCCTCTGCA GGACGTGGAC
    GTGGGGGCCG GGGAGATGGC GCTGTTTGAG TGCCTGGTGG CGGGTCCCAC CGACGTGGAG
    GTAGACTGGC TGTGCCGAGG CCGCCTGCTG CAGCCTGCAC TACTCAAATG CAAGATGCAT
    TTCGATGGCC GCAAATGCAA GCTGCTGCTC ACCTCTGTGC ATGAGGACGA CAGCGGCGTC
    TACACCTGCA AGCTCAGCAC GGCCAAAGAT GAACTGACCT GCAGTGCCCG GCTGACCGTG
    CGACCCTCGC TGGCACCCCT GTTCACGCGG CTGCTGGAGG ACGTGGAAGT GCTGGAGGGC
    CGCGCTGCCC GCTTCGACTG CAAGATCAGC GGGAGCCCGC CCCCCTCCGT TACCTGGACT
    CACTTTGGCC ACCCCGTGGA GGAGAGCGAG AACGTGCGGC TGCGGCAGGA CGGGGGCCTG
    CATTCGCTGC ACATCGCACA TGTGGGCAGT GAGGATGAGG GGCTGTACGC GGTCAGTGCT
    GCCAACACCC ACGGCCAGGC CCACTGCTCA GCCCAGCTCT ACGTGGAGGA GCCTCGGACG
    GCTGCCACCG GCCCTAGCTC CAAGCTGGAG AAGATGCCCT CCATCCCCGA GGAGCCCGAG
    CAGGGTGAGC TGGAGCGGCT GTCCATGCCC GACTTCCTGC GGCCGCTGCA GGATCTGGAC
    GTGGGACTGG CCAAGGAGGC CATGCTGGAG TGCCAGGTGA CCGGCCTGCC CTACCCCACC
    ATCAGCTGGT TCCACAACGG CCACCGCATC CAGAGCAGCG ACGACCGACG CATGACGCAG
    TACAGGGATG TCCATCGCTT GGTGTTCCCT GCCGTGGGAC CTCAGCATGC TGGTGTCTAC
    AAGAGCGTCA TTGCTAACAA GCTGGGCAAA GCCGCCTGCT ATGCTCATCT CTACGTCACA
    GATGTGGTTC CAGGCCCCCC AGACGGTGCC CCTCAGGTAG TGGCTGTGAC CGGGAGGATG
    ATCACGCTTG CATGGAACCC CCCGAGGAGT CTGGACATGG CTATCGACCC AGACGCCCTG
    ACCTACACCG TGCAGCACCA GGTGCTGGGC TCAGACCAGT GGACGGCGCT GGTCACGGGC
    CTGCGGCAGC CCGGGTGGGC AGCCACCGGA TTGCGAAAGG GGGTCCAGCA CGTCTTCCGG
    GTTCTCAGCA CCACCATCAA GAGCAGCAGC AAGCCCTCGC CCCCGTCCGA GCCCACGCAG
    CTGCTGGAGC GTGGCCCGCC CCTGGAGGAG GCGCCCGCTG TGCTGGACAA GCCAGACGTC
    GTGTATGTGG TGGAGGGCCA GCCCACCAGT GTCACCGTCA CCTTCAACCA CGTGGAGGCC
    CAGGTTGTCT GGAGGAGCTG CCGAGGAGCC CTCCTAGAGG CCCGGCCAGG TGTGTTTGAG
    CTGAGCCAGC CAGATGACGA CCAGTACTCT CTTAGGATCT GCCGGGTGAG CCGCCGGGAC
    CTGGGAGCCC TCACTTGCAC TGCCCGCAAC CGCCACGGCA CACAGGCCTG CTCAGTCACC
    CTGGAGCTGG CAGAGGCCCC TCGGTTTGAG TCCATCATGG AGGATGTGGA GGTAGGGGCT
    GGAGAGACTG CTCGCTTCGC CGTGGTGGTG GACGGGAAGC CCTTGCCGGA CATCATGTGG
    TACAAGGATG AGGCGCTGCT GACCGAGAGC AACCATGTGA GCTTCGTGTA CGAGGAGAAC
    GAGTGCTCCC TGGTGGTGCT CAGCGCGGGG GCCCAGGATG GAGGGGTCTA CACCTGCACG
    GCCCGTAACC TGGCTGGCGA AGTCTCCTGC AAAGCAGAGC TGGCTGTGCA CTCAGCTCAG
    ACAGCTATGG AGGTGGAAGG GATCGGGGAA GACGAGGAGC AGCGAGGAAG GAGGCTCAGC
    GACTTCTATG ATATTCAGCA GGAGATCGGC AGGGGTGCCT TCTCCTACCT GCGCCGTGTA
    GTGGAGAGGA GCTCCGGACT GGAGTTTGCA GCCAAGTTCA TCCCCAGCCA GGCAAAGCCA
    AAGGCATCGG CATGGCGGGA GGCACGGCTG CTGGCCCGGC TCCAGCATGA CTGCATCCTC
    TACTTCCATG AGGCCTTCGA GAGGCGCCGG GGACTGGTCA TCGTCACCGA GCTCTGCACA
    GAAGAGCTTC TGGAGCGAAT GGCCAGGAAA CCCACCGTGT GTGAGTCTGA GATCCGGGCC
    TACATGCGGC AGGTACTAGA AGGAATATGC TACTTGCACG AGAACCACGT GCTGCACCTG
    GATGTCAAGC CCGAGAACCT GCTGGTGTGG GATGGTGTTG AGGGCGAGGA GCAGGTGAGG
    ATCTGTGACT TTGGGAACGC TCAAGAGCTG ACACCGGGAG AGCCCCAGTA TTGCCAATAC
    GGCACACCTG AGTTCGTGGC GCCCGAGATT GTCAACCAGA CCCCTGTGTC TGGAGTCACT
    GACATCTGGC CCGTGGGTGT CGTCGCCTTC CTCTGCCTGA CCGGAATCTC CCCGTTCGTT
    GGGGAAAATG ATCGGACGAC ATTGATGAAC ATCCGAAACT ACAACGTGGC CTTCGAGGAG
    ACCACGTTCC TGAGCCTGAG CAGGGAGGCC CGGGGCTTCC TCATCAAAGT GCTGGTGCGG
    GACCGGCTGA GGCCTACTGC AGAGGAGACC CTGGAACATC CTTGGTTTAA AGCACAGGCA
    AAGGGTGCAG TGGTGAGCAC GGATCACCTA AAGCTATTCC TCTCCCGGCG GAGGTGGCAG
    CGCTCCCAGA TCAGCTACAA GTGCCACCTG GTGCTGCGCC CCATCCCGGA GCTGCTGCGA
    GCACCCCCGG AGCGGGTGTG GGTGGCCGTG CCCCGAAGAC CACCCCCTAG TGGAGGGCTG
    TCGTCATCAT CCGACTCTGA AGAGGAAGAG CTGGAGGAGC TGCCCTCTGT GCCCCGACCT
    CTGCAGCCTG AGTTTTCGGG CTCCCGGGTG TCCCTCACTG ACATTCCCAC TGAGGATGAG
    GCCCTCGGGG CCCTGGAGGC TGGGGCGGCC ACCCCCATGG ACTGGCAAGA ACAAGGAAAG
    GCCCCCTCTC AGGACCAGGA GGCCCCCAGC CCTCAGGCAC TCCCCTCCCC AGGCCAGGAG
    TCCCCGTCCG GGCCCAGCCC CCGGCGGGCA GAGCTCCGCA GGGGCAGCTC GGCTGAGAGC
    GCCCTGCCCC GGGCCGGGCC GAGGGAACCG GGCCGGGGCC TGCACAAGGC AGCGTCCGTC
    GAGCTGCCGC AGCGCCGAAG CCCCAGCCCG GGGGCCACCC GCCTGACCCG AGGAGGCCTG
    GGTGAGGGCG AGTATGCCCA GAGGCTGCAG GCCCTGCGCC AGCGGCTGCT GAGGGGTGGC
    CCCGATGATG GCAAGGTCAG CGGCCTCAGG GGCCCCCTGC TGGAGAGCCT GGGGGGCCGG
    GCTCGTGACC CCCGGCTGGC GCGGGCAGCC TCCAGCGAGG CAGCACCCCA TCACCAGCAC
    CCACCTGAGG CTCGGGGCCT GCAGAAGAGC AGTAGCTTCT CGCAGGGTGA GGCAGAGCCC
    CGGGGCCGGC ACCGCCGAGC AGGGGCACCC CTTGAGATCC CCGTGGCCCG GCTGGGGGCC
    CGCAAGCTGC AGGAGTCTCC CTCCTTGTCC GCCCTCAGCG AGGCCCAACC ACCTAGCCCC
    GTGCGGCCCA GTGCAGCCAA GCCCAGCACC CCTAAGTCTT CAGAACCTCT TGTGGGCACC
    CCCAGTGAGG CTGCACAGCC TCCAGCACCC CAGCCTGCCC GAGAGAAGGC CCCAGAATCT
    ACGCCAGAAC CAGCTCGGGG CCCCAAGCCC ACACAGCCCC CCACAGCTCT GCAAACCCTA
    GCGCCTCCTC TCACTCCGTA TGCGCAGATC ATGCAGTCGC TCCAGCTGCC AGGCCAGGCG
    CAAGGCCCCC CACAGGGCGC AGTCGTATCT CCTACATCTC GGTCTCCAGC ATCTCGGTTG
    GAACCCAAGC CCCACCCGGC TGTGTTCTCC AGGGTGGCCT CCCCACCTCC AGGAGCCTCC
    GAGAAGCGCC TGCTGCCGGG AGCCGGAGCA ACCCCAGGGC CAGCCGAAAA AGCCCGGGTC
    CCGGCGGTCC CCCGCAGGCC AGGGAGCAGT CTCAGCAGCA GCATCGAGAA CCTGGAGTCG
    GAGGCCGTGT TCGAGGCCAA GTTCAAGCGC AGCCGGGAGT CGCCCCTGTC GCGGGGGCTG
    CGGCTGCTGA GCCGCTCGCG GTCGGAGGAG CGCGGCCCCT TCCGCGGGGC TGAGGAGGAG
    GACGGCATTT ACCGGCCCAG CCCGGCGGGC ACGCCGCTGG AGCTGGTGCG ACGGCCCGAG
    CGCTCGCGCT CCGTGCAGGA CCTCAGGGCG GTCGGGGAGC CAGGCCTCGT GCGCCGCCTC
    TCGCTGTCGC TGTCCCAGCG TCTGCGGCGG ACCCCGCCCG CGCAGCGCCA CCCGGCCTGG
    GAGCCCCGCG GCGGGGACGG CGAGAGCTCG GAGGGCGGCA GCTCGGCGCG GGGCTCCCCG
    GTGCTGACGG TGCGCCGGAG GCTCAGCTCC ACGCTGGAGC GGCTGTCGAG CCGGTTGCAG
    CGCAGCGGCA GCAGCGAGGA CTCGGGGGGC GCGTCGGGCC GCAGCACGCC GCTGTTCGGC
    CGGCTGCGCA GGGCCACGTC CGAGAGCGAG AGTCTGCGGC GCCTGGACCT CGCGCAAAAT
    CAGCTGGCGG CCCAAGCCGG CGCCGCCACC CCTTCCGCGG AGTCCCTGGG CTCCGAGGCC
    AGCGCTACGT CGGGCTCCTC TGCTCCTGCA GAAAGTCGAA GCCGCCTGCG CTGGGGCCTC
    TCTCGGCTGC GGAAGGACAA GAGGTTGTCA CAGCCTAACC TCTCTGGCAG TGTCCAGGAG
    GATTTGGGTC ACCAGTATGT GCGCAGTGAG TCAGACTTTC CCCCAGTCTT CCACATCAAA
    CTCAAGGACC AGGTGCTGCT GGAGGGAGAG GCAGCCACCC TGCTCTGCTT GCCGGCAGCC
    TGCCCTGCAC CCCACATCTC CTGGATGAAA GACAAGCAGT CCCTGCGGTC AGAGCCCTCG
    GTGATCATCG TGTCCTGCAA AGATGGTCGT CAGCTACTGA GCATCCCCCG GGCAGGCAAG
    CGGCATGCGG GGCTCTATGA GTGCTCCGCC ACCAACGTCC TTGGCAGCAT CACCAGCTCC
    TGTACCGTGG CTGTGGCTCG TACCCCAGGA AAGCTAGCTC CTCCTGAGGT GCCCCAGACC
    TACCAGGACA CAGCACTGGT GCTCTGGAAG CCGGGAGACA GCAAGGCGCC TTGCACATAC
    ACGCTGGAGC GGCGGGTGGA TGGGGAGTCT TCCTGGCACC CTGTGAGCTC GGGCATCCCC
    GACTGCTACT ACAACGTGAC CCACCTACCA GTCGGCATGG CTGTGAGGTT CCGTGTGGCC
    TGTGCCAACC GTGCTGGGCA AGGGCCTTTC AGCAACCCTT CTGAGAAGGT CCTCGTCAGG
    GGCGTGCAAG ATTCCTCAGC TCTGCCATCT GCTGCTCACA GAGATGCCCC TGTTACCTCA
    GGGCCAGCCA GGGCCCCGCC TCCTGACGCT CCTACCTCAC TGGCCCCTCC CCTGAGCCCC
    TCCCCAGCTC CTGCTCCCCC AGGCCCCCAG GCAGCCCCTC TCGGCCCTTC ATCTTCCCCA
    GCACCCCCCA GCCAGGCCTT GTCCTCCCTC AAGGCTGTGG GTCCACCACC CCAAACCCCC
    CCACGAAAGC ACCGGGGCCT GCAGGCTGCC CAGCAAGTAG AACCTACCCC ACCCAGTGCC
    CAGGACTCCC CAAGTGAGCC CAAGTCTTCC GTCCCTGACA CTGGGACCCC CATCCCAGCC
    TCTGCCCCTC AAGTGGTGAA AACAGCCTCT TCCTCAATGC CCCTGTATAT GGTGACCTCC
    TTTGTGTCTG CACCACCAGC CCCTGAGCCC CCAGCCCCCG AGCCCCCTCC TGAGCCCACC
    AAAGTGACTG TGCGGAGCCT CAGCCCAGCC AAGGAGGTGG TTGGCTCCCC TGGGGGTGGT
    CCCCCCAGGG CTCCTAAGCC AGAGGGCACC ACTCTTCGAC AGGGCCCTCC TCAGAAACCA
    TACACCTTCC TGGAGGAGAA GGCCAGGGGT CGCTTTGGTG TGGTGCGTGC CTGCCGGGAG
    AATGCCACCG GGCGAACATT CGTGGCCAAG ATTGTGCCAT ACGCGGCCGA GGGCAAGCGG
    CAGGTCCTGC AGGAGTACGA GGTGCTGAGG ACCCTGCACC ACGAGCGGCT TATGGCCCTG
    CACGAAGCCT ACATCACCCC GCGCTACCTC GTGCTCATCG CCGAGAGCTG CGGCAACCGG
    GAGCTTCTCT GTGGGCTCAG CGACAGATTC CGGTATTCAG AGGATGATGT GGCCACCTAT
    GTGGTGCAGC TGCTACAAGG CCTGGATTAC CTCCATGGCC GCCACGTGCT GCACCTGGAC
    ATCAAGCCAG ACAACCTGCT GCTGGCCCCT GACAACGCCC TCAAGATCGT GGACTTTGGC
    AGTGCCCAGC CCTACAACCC CCAGGCTCTG CGGCCCCTGG GTCACCGCAC GGGCACACTG
    GAGTTCATGG CTCCTGAGAT GGTAAAGGGA GACCCCATCG GCTCTGCCAC GGACATCTGG
    GGAGCGGGTG TGCTCACTTA CATCATGCTC AGTGGACGCT CCCCATTCTA TGAGCCAGAC
    CCCCAGGAAA CAGAGGCTCG CATTGTGGGG GGCCGCTTTG ATGCCTTCCA GCTGTATCCC
    AACACATCCC AGAGTGCCAC CCTCTTCTTG CGAAAGGTCC TCTCAGTACA TCCCTGGAGC
    CGGCCCTCCG TGCAGGACTG CCTGGCTCAC CCGTGGCTGC AGGATGCCTA CCTGATGAAA
    CTGCGCCGCC AGACACTCAC CTTCACCACC AACCGGCTCA AGGAGTTCCT GGGTGAGCAG
    CGGCGCCGCA GGGCCGAGGC CGCCACCCGC CACAAGGTGC TGCTCCGCTC TTACCCAGGC
    AGCCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87988
    Clone ID Related Accession (Same CDS sequence) XM_015459160.1
    Accession Version XM_015459160.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product striated muscle preferentially expressed protein kinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103849.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGTCTCTCT CCTGCTGCTC CTATGGAAGC GAAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
    TACGACTCGG AGACAGGCGA GGATGACATC AGCGACGGGC AGGGGACCCA GCGCCTGGAG
    CTTCGGGATG ATGGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGCACC
    TCCCTGGACA CGCCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG
    GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCA GGGCTGGCAC CCGCCGCCTC
    CCGGGCAGCC CAAGGCAAGC ACAGGCAACC GGGGCCGGGC CACGGCACCT GGGGGTGGAG
    CCGCTCGTAC GGGCATCTCG AGCTAATCTG GTGGGCGCAA GCTGGGGGTC AGAGGATAGC
    CTTTCGGTGG CCAGTGACCT GTACGGCAGC GCATTCAGCC TGTACAGAGG ACGGGCGCTC
    TCGATCCACG TCAGTGTCCC TCAGAGCGGA CTGCACAAGG AGGAGCCTGA CCTTCAGCCT
    CAGCCCGCCA GCGAAGCCCC CCGTCGCCCA GCCCTGCCGC CCCCCTCCAA ATCCGCGCTG
    CTGCCCCCAC CGTCCCCTCG GGTGGGTAAG CGGCCCCCGC CGGGAGCCGG CGGCCAGCCC
    CTGGCCACCC CCACGCCGCC GCACCGGCGC TCTCGGGAGC CGGTGCCGCC CGAGGACGCC
    ACCGCCGAAG AAAAGCGAGG GAAAAAGTCC AAGTCGTCCG GGCCCTCGCT AGCGGGCACG
    GCGGAGTCCC GGCCCCAGAC GCCCCTGAGT GAGGCCTCGG GCCGCCAGTC GGCGCTGGGC
    CGCTCCCCGA GGCTGGTGCG CGCTGGCTCC CGCATCCTGG ACAAGCTGCA GTTCTTCGAG
    GAGCGCCGGC GCAGCCTGGA GCGCAGCGAC TCGCCGCCGG CGCCCCTGCG GCCCTGGGTG
    CCCCTGCGCA AGGCCCGTTC GCTGGAGCAG CCCAAGTCCG AGGGCGGCGG GCCGTGGGGC
    ACGCCCAGGG CCTCGCAGGA GGAGCTGCGG TCCCCGGCGG GCAGCGTGGC CGAGCGGCGC
    CGCCTATTCC AACAGAAGGC GGCCTCGCTG GACGAGCGCA CGCGTCAGCG CAGCCCTGCG
    TCCGACCTCG AGCTGCGCTT CGCCCAGGAG CTGGGCCGCA TCCGCCGCTC CACGTCGCGG
    GAGGAGCTGG TGCGCTCTCA CGAGTCCCTG CGCGCCACGC TGCAGCGCGC CCCGTCCCCC
    CGAGAGCCCG GCGAGCCGCC GCTCTTCTCC CAGCCCTCCA CCCCCAAGAC CCCGCGGGCC
    TTGAGCCCCG CCGCCGCCCA GCCGCCCCCT GCGAGCGTCG CGGAAAAGCC CGGGGATGAG
    CCAGGGAGGC CCCGGGGCCG TGGGCCAGCG GGCAGGACGG AGCCGGGGGA AGGCCCGCAG
    CAGGAGGTTA GGCGCCGGGA CCAGTTCCCG CTGACCCGGA GCAGAGCCAT CCAGGAGTGC
    CGGAGCCCGG TGCCGCCCCC CACCTCTGAG CCCCCCGAGA CCAGGACGAA AGCTCCCCCG
    GGTCGGAAGC GGGAGCCCCC GCCGCAGGCC GTGCGCTTCC TGCCCTGGGC CACGCCGGGC
    CAGGAGGGCG CTGCTGTGCC CCAGACCTTG GAGAAGAACA GGGCGGGACC CGAGGCCGAG
    AAGAGGCTGC GCAGAGGGCC AGAGGAGGAC GGTCCTTGGG GGGCCTGGGA CCGCCGAGGG
    GCCCGCAGCC AGGGCAGAGG TCGCCGGGCG CGGCCCACCT CGCCTGAGCT CGAGTCTTCG
    GATGACTCCT ATGTGTCTGC TGGAGAAGAG CCTCTGGAGG CCCCTGTGTT TGAGATCCCC
    CTGCAAAATG CAGTGGTGGC CCCAGGGGTG GACGTGCTGC TCAAGTGTAT TGTTACCGCC
    AACCCCCCGC CCCAAGTGTC CTGGCAGAAG GATGGGTCCC CGCTGCGCAG TGATGGCCGC
    CTTCTCATCC GGGCAGAAGG CGAGCGGCAC ACCCTGCTGC TCCGGGAGGC CCGGGCCGCC
    GATGCCGGGA GCTATGCAGC CACTGCCACC AACGAGCTGG GCCAGGCCCG CTGCGCTGCC
    ACGCTGGCCG TGAGACCTGG TGGGTCAACA TCTCCTTTCA GCAGTCCTAT CACCTCTGAC
    GAGGAGTATC TGAGCCCCCC AGAGGAGTTC CCGGAACCCG GGGAGACCTG GCCCCGAGCC
    CCCACCATGA AGCTCAGTCC TGGCCAGAAT CGCCGTTCCT CCGACACTGG CTCCAAGGCG
    CCCCCTACTT TCAAGGTTTC ACTTATGGAC CAGTCAGTAA GGGAAGGCCA AGACGTCACC
    ATGAGCATCC GTGTGCAGGG CGAACCCAAG CCGGTGGTCT CCTGGCTGAG AAACCGTCAG
    CCTGTGCGCC CAGACCAGCG GCGCTTTGCT GAGGAGGCAG AGGATGGGCT CTGCCGGCTT
    CGGATCCTGG CTGCCGAGCG GGGCGACGCT GGCTTCTACA CCTGCAAGGC GGTTAACGAG
    TATGGCGCTC GACAGTGTGA GGCGCGCCTG GAGGTCAGAG CACACCCTGA AAGCCGGTCC
    CTGGCCGTGC TGGCCCCTCT GCAGGACGTG GACGTGGGGG CCGGGGAGAT GGCGCTGTTT
    GAGTGCCTGG TGGCGGGTCC CACCGACGTG GAGGTAGACT GGCTGTGCCG AGGCCGCCTG
    CTGCAGCCTG CACTACTCAA ATGCAAGATG CATTTCGATG GCCGCAAATG CAAGCTGCTG
    CTCACCTCTG TGCATGAGGA CGACAGCGGC GTCTACACCT GCAAGCTCAG CACGGCCAAA
    GATGAACTGA CCTGCAGTGC CCGGCTGACC GTGCGACCCT CGCTGGCACC CCTGTTCACG
    CGGCTGCTGG AGGACGTGGA AGTGCTGGAG GGCCGCGCTG CCCGCTTCGA CTGCAAGATC
    AGCGGGAGCC CGCCCCCCTC CGTTACCTGG ACTCACTTTG GCCACCCCGT GGAGGAGAGC
    GAGAACGTGC GGCTGCGGCA GGACGGGGGC CTGCATTCGC TGCACATCGC ACATGTGGGC
    AGTGAGGATG AGGGGCTGTA CGCGGTCAGT GCTGCCAACA CCCACGGCCA GGCCCACTGC
    TCAGCCCAGC TCTACGTGGA GGAGCCTCGG ACGGCTGCCA CCGGCCCTAG CTCCAAGCTG
    GAGAAGATGC CCTCCATCCC CGAGGAGCCC GAGCAGGGTG AGCTGGAGCG GCTGTCCATG
    CCCGACTTCC TGCGGCCGCT GCAGGATCTG GACGTGGGAC TGGCCAAGGA GGCCATGCTG
    GAGTGCCAGG TGACCGGCCT GCCCTACCCC ACCATCAGCT GGTTCCACAA CGGCCACCGC
    ATCCAGAGCA GCGACGACCG ACGCATGACG CAGTACAGGG ATGTCCATCG CTTGGTGTTC
    CCTGCCGTGG GACCTCAGCA TGCTGGTGTC TACAAGAGCG TCATTGCTAA CAAGCTGGGC
    AAAGCCGCCT GCTATGCTCA TCTCTACGTC ACAGATGTGG TTCCAGGCCC CCCAGACGGT
    GCCCCTCAGG TAGTGGCTGT GACCGGGAGG ATGATCACGC TTGCATGGAA CCCCCCGAGG
    AGTCTGGACA TGGCTATCGA CCCAGACGCC CTGACCTACA CCGTGCAGCA CCAGGTGCTG
    GGCTCAGACC AGTGGACGGC GCTGGTCACG GGCCTGCGGC AGCCCGGGTG GGCAGCCACC
    GGATTGCGAA AGGGGGTCCA GCACGTCTTC CGGGTTCTCA GCACCACCAT CAAGAGCAGC
    AGCAAGCCCT CGCCCCCGTC CGAGCCCACG CAGCTGCTGG AGCGTGGCCC GCCCCTGGAG
    GAGGCGCCCG CTGTGCTGGA CAAGCCAGAC GTCGTGTATG TGGTGGAGGG CCAGCCCACC
    AGTGTCACCG TCACCTTCAA CCACGTGGAG GCCCAGGTTG TCTGGAGGAG CTGCCGAGGA
    GCCCTCCTAG AGGCCCGGCC AGGTGTGTTT GAGCTGAGCC AGCCAGATGA CGACCAGTAC
    TCTCTTAGGA TCTGCCGGGT GAGCCGCCGG GACCTGGGAG CCCTCACTTG CACTGCCCGC
    AACCGCCACG GCACACAGGC CTGCTCAGTC ACCCTGGAGC TGGCAGAGGC CCCTCGGTTT
    GAGTCCATCA TGGAGGATGT GGAGGTAGGG GCTGGAGAGA CTGCTCGCTT CGCCGTGGTG
    GTGGACGGGA AGCCCTTGCC GGACATCATG TGGTACAAGG ATGAGGCGCT GCTGACCGAG
    AGCAACCATG TGAGCTTCGT GTACGAGGAG AACGAGTGCT CCCTGGTGGT GCTCAGCGCG
    GGGGCCCAGG ATGGAGGGGT CTACACCTGC ACGGCCCGTA ACCTGGCTGG CGAAGTCTCC
    TGCAAAGCAG AGCTGGCTGT GCACTCAGCT CAGACAGCTA TGGAGGTGGA AGGGATCGGG
    GAAGACGAGG AGCAGCGAGG AAGGAGGCTC AGCGACTTCT ATGATATTCA GCAGGAGATC
    GGCAGGGGTG CCTTCTCCTA CCTGCGCCGT GTAGTGGAGA GGAGCTCCGG ACTGGAGTTT
    GCAGCCAAGT TCATCCCCAG CCAGGCAAAG CCAAAGGCAT CGGCATGGCG GGAGGCACGG
    CTGCTGGCCC GGCTCCAGCA TGACTGCATC CTCTACTTCC ATGAGGCCTT CGAGAGGCGC
    CGGGGACTGG TCATCGTCAC CGAGCTCTGC ACAGAAGAGC TTCTGGAGCG AATGGCCAGG
    AAACCCACCG TGTGTGAGTC TGAGATCCGG GCCTACATGC GGCAGGTACT AGAAGGAATA
    TGCTACTTGC ACGAGAACCA CGTGCTGCAC CTGGATGTCA AGCCCGAGAA CCTGCTGGTG
    TGGGATGGTG TTGAGGGCGA GGAGCAGGTG AGGATCTGTG ACTTTGGGAA CGCTCAAGAG
    CTGACACCGG GAGAGCCCCA GTATTGCCAA TACGGCACAC CTGAGTTCGT GGCGCCCGAG
    ATTGTCAACC AGACCCCTGT GTCTGGAGTC ACTGACATCT GGCCCGTGGG TGTCGTCGCC
    TTCCTCTGCC TGACCGGAAT CTCCCCGTTC GTTGGGGAAA ATGATCGGAC GACATTGATG
    AACATCCGAA ACTACAACGT GGCCTTCGAG GAGACCACGT TCCTGAGCCT GAGCAGGGAG
    GCCCGGGGCT TCCTCATCAA AGTGCTGGTG CGGGACCGGC TGAGGCCTAC TGCAGAGGAG
    ACCCTGGAAC ATCCTTGGTT TAAAGCACAG GCAAAGGGTG CAGTGGTGAG CACGGATCAC
    CTAAAGCTAT TCCTCTCCCG GCGGAGGTGG CAGCGCTCCC AGATCAGCTA CAAGTGCCAC
    CTGGTGCTGC GCCCCATCCC GGAGCTGCTG CGAGCACCCC CGGAGCGGGT GTGGGTGGCC
    GTGCCCCGAA GACCACCCCC TAGTGGAGGG CTGTCGTCAT CATCCGACTC TGAAGAGGAA
    GAGCTGGAGG AGCTGCCCTC TGTGCCCCGA CCTCTGCAGC CTGAGTTTTC GGGCTCCCGG
    GTGTCCCTCA CTGACATTCC CACTGAGGAT GAGGCCCTCG GGGCCCTGGA GGCTGGGGCG
    GCCACCCCCA TGGACTGGCA AGAACAAGGA AAGGCCCCCT CTCAGGACCA GGAGGCCCCC
    AGCCCTCAGG CACTCCCCTC CCCAGGCCAG GAGTCCCCGT CCGGGCCCAG CCCCCGGCGG
    GCAGAGCTCC GCAGGGGCAG CTCGGCTGAG AGCGCCCTGC CCCGGGCCGG GCCGAGGGAA
    CCGGGCCGGG GCCTGCACAA GGCAGCGTCC GTCGAGCTGC CGCAGCGCCG AAGCCCCAGC
    CCGGGGGCCA CCCGCCTGAC CCGAGGAGGC CTGGGTGAGG GCGAGTATGC CCAGAGGCTG
    CAGGCCCTGC GCCAGCGGCT GCTGAGGGGT GGCCCCGATG ATGGCAAGGT CAGCGGCCTC
    AGGGGCCCCC TGCTGGAGAG CCTGGGGGGC CGGGCTCGTG ACCCCCGGCT GGCGCGGGCA
    GCCTCCAGCG AGGCAGCACC CCATCACCAG CACCCACCTG AGGCTCGGGG CCTGCAGAAG
    AGCAGTAGCT TCTCGCAGGG TGAGGCAGAG CCCCGGGGCC GGCACCGCCG AGCAGGGGCA
    CCCCTTGAGA TCCCCGTGGC CCGGCTGGGG GCCCGCAAGC TGCAGGAGTC TCCCTCCTTG
    TCCGCCCTCA GCGAGGCCCA ACCACCTAGC CCCGTGCGGC CCAGTGCAGC CAAGCCCAGC
    ACCCCTAAGT CTTCAGAACC TCTTGTGGGC ACCCCCAGTG AGGCTGCACA GCCTCCAGCA
    CCCCAGCCTG CCCGAGAGAA GGCCCCAGAA TCTACGCCAG AACCAGCTCG GGGCCCCAAG
    CCCACACAGC CCCCCACAGC TCTGCAAACC CTAGCGCCTC CTCTCACTCC GTATGCGCAG
    ATCATGCAGT CGCTCCAGCT GCCAGGCCAG GCGCAAGGCC CCCCACAGGG CGCAGTCGTA
    TCTCCTACAT CTCGGTCTCC AGCATCTCGG TTGGAACCCA AGCCCCACCC GGCTGTGTTC
    TCCAGGGTGG CCTCCCCACC TCCAGGAGCC TCCGAGAAGC GCCTGCTGCC GGGAGCCGGA
    GCAACCCCAG GGCCAGCCGA AAAAGCCCGG GTCCCGGCGG TCCCCCGCAG GCCAGGGAGC
    AGTCTCAGCA GCAGCATCGA GAACCTGGAG TCGGAGGCCG TGTTCGAGGC CAAGTTCAAG
    CGCAGCCGGG AGTCGCCCCT GTCGCGGGGG CTGCGGCTGC TGAGCCGCTC GCGGTCGGAG
    GAGCGCGGCC CCTTCCGCGG GGCTGAGGAG GAGGACGGCA TTTACCGGCC CAGCCCGGCG
    GGCACGCCGC TGGAGCTGGT GCGACGGCCC GAGCGCTCGC GCTCCGTGCA GGACCTCAGG
    GCGGTCGGGG AGCCAGGCCT CGTGCGCCGC CTCTCGCTGT CGCTGTCCCA GCGTCTGCGG
    CGGACCCCGC CCGCGCAGCG CCACCCGGCC TGGGAGCCCC GCGGCGGGGA CGGCGAGAGC
    TCGGAGGGCG GCAGCTCGGC GCGGGGCTCC CCGGTGCTGA CGGTGCGCCG GAGGCTCAGC
    TCCACGCTGG AGCGGCTGTC GAGCCGGTTG CAGCGCAGCG GCAGCAGCGA GGACTCGGGG
    GGCGCGTCGG GCCGCAGCAC GCCGCTGTTC GGCCGGCTGC GCAGGGCCAC GTCCGAGAGC
    GAGAGTCTGC GGCGCCTGGA CCTCGCGCAA AATCAGCTGG CGGCCCAAGC CGGCGCCGCC
    ACCCCTTCCG CGGAGTCCCT GGGCTCCGAG GCCAGCGCTA CGTCGGGCTC CTCTGCTCCT
    GCAGAAAGTC GAAGCCGCCT GCGCTGGGGC CTCTCTCGGC TGCGGAAGGA CAAGAGGTTG
    TCACAGCCTA ACCTCTCTGG CAGTGTCCAG GAGGATTTGG GTCACCAGTA TGTGCGCAGT
    GAGTCAGACT TTCCCCCAGT CTTCCACATC AAACTCAAGG ACCAGGTGCT GCTGGAGGGA
    GAGGCAGCCA CCCTGCTCTG CTTGCCGGCA GCCTGCCCTG CACCCCACAT CTCCTGGATG
    AAAGACAAGC AGTCCCTGCG GTCAGAGCCC TCGGTGATCA TCGTGTCCTG CAAAGATGGT
    CGTCAGCTAC TGAGCATCCC CCGGGCAGGC AAGCGGCATG CGGGGCTCTA TGAGTGCTCC
    GCCACCAACG TCCTTGGCAG CATCACCAGC TCCTGTACCG TGGCTGTGGC TCGTACCCCA
    GGAAAGCTAG CTCCTCCTGA GGTGCCCCAG ACCTACCAGG ACACAGCACT GGTGCTCTGG
    AAGCCGGGAG ACAGCAAGGC GCCTTGCACA TACACGCTGG AGCGGCGGGT GGATGGGGAG
    TCTTCCTGGC ACCCTGTGAG CTCGGGCATC CCCGACTGCT ACTACAACGT GACCCACCTA
    CCAGTCGGCA TGGCTGTGAG GTTCCGTGTG GCCTGTGCCA ACCGTGCTGG GCAAGGGCCT
    TTCAGCAACC CTTCTGAGAA GGTCCTCGTC AGGGGCGTGC AAGATTCCTC AGCTCTGCCA
    TCTGCTGCTC ACAGAGATGC CCCTGTTACC TCAGGGCCAG CCAGGGCCCC GCCTCCTGAC
    GCTCCTACCT CACTGGCCCC TCCCCTGAGC CCCTCCCCAG CTCCTGCTCC CCCAGGCCCC
    CAGGCAGCCC CTCTCGGCCC TTCATCTTCC CCAGCACCCC CCAGCCAGGC CTTGTCCTCC
    CTCAAGGCTG TGGGTCCACC ACCCCAAACC CCCCCACGAA AGCACCGGGG CCTGCAGGCT
    GCCCAGCAAG TAGAACCTAC CCCACCCAGT GCCCAGGACT CCCCAAGTGA GCCCAAGTCT
    TCCGTCCCTG ACACTGGGAC CCCCATCCCA GCCTCTGCCC CTCAAGTGGT GAAAACAGCC
    TCTTCCTCAA TGCCCCTGTA TATGGTGACC TCCTTTGTGT CTGCACCACC AGCCCCTGAG
    CCCCCAGCCC CCGAGCCCCC TCCTGAGCCC ACCAAAGTGA CTGTGCGGAG CCTCAGCCCA
    GCCAAGGAGG TGGTTGGCTC CCCTGGGGGT GGTCCCCCCA GGGCTCCTAA GCCAGAGGGC
    ACCACTCTTC GACAGGGCCC TCCTCAGAAA CCATACACCT TCCTGGAGGA GAAGGCCAGG
    GGTCGCTTTG GTGTGGTGCG TGCCTGCCGG GAGAATGCCA CCGGGCGAAC ATTCGTGGCC
    AAGATTGTGC CATACGCGGC CGAGGGCAAG CGGCAGGTCC TGCAGGAGTA CGAGGTGCTG
    AGGACCCTGC ACCACGAGCG GCTTATGGCC CTGCACGAAG CCTACATCAC CCCGCGCTAC
    CTCGTGCTCA TCGCCGAGAG CTGCGGCAAC CGGGAGCTTC TCTGTGGGCT CAGCGACAGA
    TTCCGGTATT CAGAGGATGA TGTGGCCACC TATGTGGTGC AGCTGCTACA AGGCCTGGAT
    TACCTCCATG GCCGCCACGT GCTGCACCTG GACATCAAGC CAGACAACCT GCTGCTGGCC
    CCTGACAACG CCCTCAAGAT CGTGGACTTT GGCAGTGCCC AGCCCTACAA CCCCCAGGCT
    CTGCGGCCCC TGGGTCACCG CACGGGCACA CTGGAGTTCA TGGCTCCTGA GATGGTAAAG
    GGAGACCCCA TCGGCTCTGC CACGGACATC TGGGGAGCGG GTGTGCTCAC TTACATCATG
    CTCAGTGGAC GCTCCCCATT CTATGAGCCA GACCCCCAGG AAACAGAGGC TCGCATTGTG
    GGGGGCCGCT TTGATGCCTT CCAGCTGTAT CCCAACACAT CCCAGAGTGC CACCCTCTTC
    TTGCGAAAGG TCCTCTCAGT ACATCCCTGG AGCCGGCCCT CCGTGCAGGA CTGCCTGGCT
    CACCCGTGGC TGCAGGATGC CTACCTGATG AAACTGCGCC GCCAGACACT CACCTTCACC
    ACCAACCGGC TCAAGGAGTT CCTGGGTGAG CAGCGGCGCC GCAGGGCCGA GGCCGCCACC
    CGCCACAAGG TGCTGCTCCG CTCTTACCCA GGCAGCCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314646.1
    CDS982..10503
    Translation

    Target ORF information:

    RefSeq Version XM_015459160.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SPEG complex locus (SPEG), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459160.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGTCTCTCT CCTGCTGCTC CTATGGAAGC GAAGTTTTCC GCTCCTGCAG AAAGCAAAGT 
    TACGACTCGG AGACAGGCGA GGATGACATC AGCGACGGGC AGGGGACCCA GCGCCTGGAG
    CTTCGGGATG ATGGGGCCTT CAGCACCCCC ACGGGGGGCT CTGATACCCT GGTGGGCACC
    TCCCTGGACA CGCCCCCGAC CTCCGTGACA GGCACCTCAG AGGAGCAAGT GAGCTGGTGG
    GGCAGCGGGC AGACGGTCCT GGAGCAGGAA GCGGGCAGCA GGGCTGGCAC CCGCCGCCTC
    CCGGGCAGCC CAAGGCAAGC ACAGGCAACC GGGGCCGGGC CACGGCACCT GGGGGTGGAG
    CCGCTCGTAC GGGCATCTCG AGCTAATCTG GTGGGCGCAA GCTGGGGGTC AGAGGATAGC
    CTTTCGGTGG CCAGTGACCT GTACGGCAGC GCATTCAGCC TGTACAGAGG ACGGGCGCTC
    TCGATCCACG TCAGTGTCCC TCAGAGCGGA CTGCACAAGG AGGAGCCTGA CCTTCAGCCT
    CAGCCCGCCA GCGAAGCCCC CCGTCGCCCA GCCCTGCCGC CCCCCTCCAA ATCCGCGCTG
    CTGCCCCCAC CGTCCCCTCG GGTGGGTAAG CGGCCCCCGC CGGGAGCCGG CGGCCAGCCC
    CTGGCCACCC CCACGCCGCC GCACCGGCGC TCTCGGGAGC CGGTGCCGCC CGAGGACGCC
    ACCGCCGAAG AAAAGCGAGG GAAAAAGTCC AAGTCGTCCG GGCCCTCGCT AGCGGGCACG
    GCGGAGTCCC GGCCCCAGAC GCCCCTGAGT GAGGCCTCGG GCCGCCAGTC GGCGCTGGGC
    CGCTCCCCGA GGCTGGTGCG CGCTGGCTCC CGCATCCTGG ACAAGCTGCA GTTCTTCGAG
    GAGCGCCGGC GCAGCCTGGA GCGCAGCGAC TCGCCGCCGG CGCCCCTGCG GCCCTGGGTG
    CCCCTGCGCA AGGCCCGTTC GCTGGAGCAG CCCAAGTCCG AGGGCGGCGG GCCGTGGGGC
    ACGCCCAGGG CCTCGCAGGA GGAGCTGCGG TCCCCGGCGG GCAGCGTGGC CGAGCGGCGC
    CGCCTATTCC AACAGAAGGC GGCCTCGCTG GACGAGCGCA CGCGTCAGCG CAGCCCTGCG
    TCCGACCTCG AGCTGCGCTT CGCCCAGGAG CTGGGCCGCA TCCGCCGCTC CACGTCGCGG
    GAGGAGCTGG TGCGCTCTCA CGAGTCCCTG CGCGCCACGC TGCAGCGCGC CCCGTCCCCC
    CGAGAGCCCG GCGAGCCGCC GCTCTTCTCC CAGCCCTCCA CCCCCAAGAC CCCGCGGGCC
    TTGAGCCCCG CCGCCGCCCA GCCGCCCCCT GCGAGCGTCG CGGAAAAGCC CGGGGATGAG
    CCAGGGAGGC CCCGGGGCCG TGGGCCAGCG GGCAGGACGG AGCCGGGGGA AGGCCCGCAG
    CAGGAGGTTA GGCGCCGGGA CCAGTTCCCG CTGACCCGGA GCAGAGCCAT CCAGGAGTGC
    CGGAGCCCGG TGCCGCCCCC CACCTCTGAG CCCCCCGAGA CCAGGACGAA AGCTCCCCCG
    GGTCGGAAGC GGGAGCCCCC GCCGCAGGCC GTGCGCTTCC TGCCCTGGGC CACGCCGGGC
    CAGGAGGGCG CTGCTGTGCC CCAGACCTTG GAGAAGAACA GGGCGGGACC CGAGGCCGAG
    AAGAGGCTGC GCAGAGGGCC AGAGGAGGAC GGTCCTTGGG GGGCCTGGGA CCGCCGAGGG
    GCCCGCAGCC AGGGCAGAGG TCGCCGGGCG CGGCCCACCT CGCCTGAGCT CGAGTCTTCG
    GATGACTCCT ATGTGTCTGC TGGAGAAGAG CCTCTGGAGG CCCCTGTGTT TGAGATCCCC
    CTGCAAAATG CAGTGGTGGC CCCAGGGGTG GACGTGCTGC TCAAGTGTAT TGTTACCGCC
    AACCCCCCGC CCCAAGTGTC CTGGCAGAAG GATGGGTCCC CGCTGCGCAG TGATGGCCGC
    CTTCTCATCC GGGCAGAAGG CGAGCGGCAC ACCCTGCTGC TCCGGGAGGC CCGGGCCGCC
    GATGCCGGGA GCTATGCAGC CACTGCCACC AACGAGCTGG GCCAGGCCCG CTGCGCTGCC
    ACGCTGGCCG TGAGACCTGG TGGGTCAACA TCTCCTTTCA GCAGTCCTAT CACCTCTGAC
    GAGGAGTATC TGAGCCCCCC AGAGGAGTTC CCGGAACCCG GGGAGACCTG GCCCCGAGCC
    CCCACCATGA AGCTCAGTCC TGGCCAGAAT CGCCGTTCCT CCGACACTGG CTCCAAGGCG
    CCCCCTACTT TCAAGGTTTC ACTTATGGAC CAGTCAGTAA GGGAAGGCCA AGACGTCACC
    ATGAGCATCC GTGTGCAGGG CGAACCCAAG CCGGTGGTCT CCTGGCTGAG AAACCGTCAG
    CCTGTGCGCC CAGACCAGCG GCGCTTTGCT GAGGAGGCAG AGGATGGGCT CTGCCGGCTT
    CGGATCCTGG CTGCCGAGCG GGGCGACGCT GGCTTCTACA CCTGCAAGGC GGTTAACGAG
    TATGGCGCTC GACAGTGTGA GGCGCGCCTG GAGGTCAGAG CACACCCTGA AAGCCGGTCC
    CTGGCCGTGC TGGCCCCTCT GCAGGACGTG GACGTGGGGG CCGGGGAGAT GGCGCTGTTT
    GAGTGCCTGG TGGCGGGTCC CACCGACGTG GAGGTAGACT GGCTGTGCCG AGGCCGCCTG
    CTGCAGCCTG CACTACTCAA ATGCAAGATG CATTTCGATG GCCGCAAATG CAAGCTGCTG
    CTCACCTCTG TGCATGAGGA CGACAGCGGC GTCTACACCT GCAAGCTCAG CACGGCCAAA
    GATGAACTGA CCTGCAGTGC CCGGCTGACC GTGCGACCCT CGCTGGCACC CCTGTTCACG
    CGGCTGCTGG AGGACGTGGA AGTGCTGGAG GGCCGCGCTG CCCGCTTCGA CTGCAAGATC
    AGCGGGAGCC CGCCCCCCTC CGTTACCTGG ACTCACTTTG GCCACCCCGT GGAGGAGAGC
    GAGAACGTGC GGCTGCGGCA GGACGGGGGC CTGCATTCGC TGCACATCGC ACATGTGGGC
    AGTGAGGATG AGGGGCTGTA CGCGGTCAGT GCTGCCAACA CCCACGGCCA GGCCCACTGC
    TCAGCCCAGC TCTACGTGGA GGAGCCTCGG ACGGCTGCCA CCGGCCCTAG CTCCAAGCTG
    GAGAAGATGC CCTCCATCCC CGAGGAGCCC GAGCAGGGTG AGCTGGAGCG GCTGTCCATG
    CCCGACTTCC TGCGGCCGCT GCAGGATCTG GACGTGGGAC TGGCCAAGGA GGCCATGCTG
    GAGTGCCAGG TGACCGGCCT GCCCTACCCC ACCATCAGCT GGTTCCACAA CGGCCACCGC
    ATCCAGAGCA GCGACGACCG ACGCATGACG CAGTACAGGG ATGTCCATCG CTTGGTGTTC
    CCTGCCGTGG GACCTCAGCA TGCTGGTGTC TACAAGAGCG TCATTGCTAA CAAGCTGGGC
    AAAGCCGCCT GCTATGCTCA TCTCTACGTC ACAGATGTGG TTCCAGGCCC CCCAGACGGT
    GCCCCTCAGG TAGTGGCTGT GACCGGGAGG ATGATCACGC TTGCATGGAA CCCCCCGAGG
    AGTCTGGACA TGGCTATCGA CCCAGACGCC CTGACCTACA CCGTGCAGCA CCAGGTGCTG
    GGCTCAGACC AGTGGACGGC GCTGGTCACG GGCCTGCGGC AGCCCGGGTG GGCAGCCACC
    GGATTGCGAA AGGGGGTCCA GCACGTCTTC CGGGTTCTCA GCACCACCAT CAAGAGCAGC
    AGCAAGCCCT CGCCCCCGTC CGAGCCCACG CAGCTGCTGG AGCGTGGCCC GCCCCTGGAG
    GAGGCGCCCG CTGTGCTGGA CAAGCCAGAC GTCGTGTATG TGGTGGAGGG CCAGCCCACC
    AGTGTCACCG TCACCTTCAA CCACGTGGAG GCCCAGGTTG TCTGGAGGAG CTGCCGAGGA
    GCCCTCCTAG AGGCCCGGCC AGGTGTGTTT GAGCTGAGCC AGCCAGATGA CGACCAGTAC
    TCTCTTAGGA TCTGCCGGGT GAGCCGCCGG GACCTGGGAG CCCTCACTTG CACTGCCCGC
    AACCGCCACG GCACACAGGC CTGCTCAGTC ACCCTGGAGC TGGCAGAGGC CCCTCGGTTT
    GAGTCCATCA TGGAGGATGT GGAGGTAGGG GCTGGAGAGA CTGCTCGCTT CGCCGTGGTG
    GTGGACGGGA AGCCCTTGCC GGACATCATG TGGTACAAGG ATGAGGCGCT GCTGACCGAG
    AGCAACCATG TGAGCTTCGT GTACGAGGAG AACGAGTGCT CCCTGGTGGT GCTCAGCGCG
    GGGGCCCAGG ATGGAGGGGT CTACACCTGC ACGGCCCGTA ACCTGGCTGG CGAAGTCTCC
    TGCAAAGCAG AGCTGGCTGT GCACTCAGCT CAGACAGCTA TGGAGGTGGA AGGGATCGGG
    GAAGACGAGG AGCAGCGAGG AAGGAGGCTC AGCGACTTCT ATGATATTCA GCAGGAGATC
    GGCAGGGGTG CCTTCTCCTA CCTGCGCCGT GTAGTGGAGA GGAGCTCCGG ACTGGAGTTT
    GCAGCCAAGT TCATCCCCAG CCAGGCAAAG CCAAAGGCAT CGGCATGGCG GGAGGCACGG
    CTGCTGGCCC GGCTCCAGCA TGACTGCATC CTCTACTTCC ATGAGGCCTT CGAGAGGCGC
    CGGGGACTGG TCATCGTCAC CGAGCTCTGC ACAGAAGAGC TTCTGGAGCG AATGGCCAGG
    AAACCCACCG TGTGTGAGTC TGAGATCCGG GCCTACATGC GGCAGGTACT AGAAGGAATA
    TGCTACTTGC ACGAGAACCA CGTGCTGCAC CTGGATGTCA AGCCCGAGAA CCTGCTGGTG
    TGGGATGGTG TTGAGGGCGA GGAGCAGGTG AGGATCTGTG ACTTTGGGAA CGCTCAAGAG
    CTGACACCGG GAGAGCCCCA GTATTGCCAA TACGGCACAC CTGAGTTCGT GGCGCCCGAG
    ATTGTCAACC AGACCCCTGT GTCTGGAGTC ACTGACATCT GGCCCGTGGG TGTCGTCGCC
    TTCCTCTGCC TGACCGGAAT CTCCCCGTTC GTTGGGGAAA ATGATCGGAC GACATTGATG
    AACATCCGAA ACTACAACGT GGCCTTCGAG GAGACCACGT TCCTGAGCCT GAGCAGGGAG
    GCCCGGGGCT TCCTCATCAA AGTGCTGGTG CGGGACCGGC TGAGGCCTAC TGCAGAGGAG
    ACCCTGGAAC ATCCTTGGTT TAAAGCACAG GCAAAGGGTG CAGTGGTGAG CACGGATCAC
    CTAAAGCTAT TCCTCTCCCG GCGGAGGTGG CAGCGCTCCC AGATCAGCTA CAAGTGCCAC
    CTGGTGCTGC GCCCCATCCC GGAGCTGCTG CGAGCACCCC CGGAGCGGGT GTGGGTGGCC
    GTGCCCCGAA GACCACCCCC TAGTGGAGGG CTGTCGTCAT CATCCGACTC TGAAGAGGAA
    GAGCTGGAGG AGCTGCCCTC TGTGCCCCGA CCTCTGCAGC CTGAGTTTTC GGGCTCCCGG
    GTGTCCCTCA CTGACATTCC CACTGAGGAT GAGGCCCTCG GGGCCCTGGA GGCTGGGGCG
    GCCACCCCCA TGGACTGGCA AGAACAAGGA AAGGCCCCCT CTCAGGACCA GGAGGCCCCC
    AGCCCTCAGG CACTCCCCTC CCCAGGCCAG GAGTCCCCGT CCGGGCCCAG CCCCCGGCGG
    GCAGAGCTCC GCAGGGGCAG CTCGGCTGAG AGCGCCCTGC CCCGGGCCGG GCCGAGGGAA
    CCGGGCCGGG GCCTGCACAA GGCAGCGTCC GTCGAGCTGC CGCAGCGCCG AAGCCCCAGC
    CCGGGGGCCA CCCGCCTGAC CCGAGGAGGC CTGGGTGAGG GCGAGTATGC CCAGAGGCTG
    CAGGCCCTGC GCCAGCGGCT GCTGAGGGGT GGCCCCGATG ATGGCAAGGT CAGCGGCCTC
    AGGGGCCCCC TGCTGGAGAG CCTGGGGGGC CGGGCTCGTG ACCCCCGGCT GGCGCGGGCA
    GCCTCCAGCG AGGCAGCACC CCATCACCAG CACCCACCTG AGGCTCGGGG CCTGCAGAAG
    AGCAGTAGCT TCTCGCAGGG TGAGGCAGAG CCCCGGGGCC GGCACCGCCG AGCAGGGGCA
    CCCCTTGAGA TCCCCGTGGC CCGGCTGGGG GCCCGCAAGC TGCAGGAGTC TCCCTCCTTG
    TCCGCCCTCA GCGAGGCCCA ACCACCTAGC CCCGTGCGGC CCAGTGCAGC CAAGCCCAGC
    ACCCCTAAGT CTTCAGAACC TCTTGTGGGC ACCCCCAGTG AGGCTGCACA GCCTCCAGCA
    CCCCAGCCTG CCCGAGAGAA GGCCCCAGAA TCTACGCCAG AACCAGCTCG GGGCCCCAAG
    CCCACACAGC CCCCCACAGC TCTGCAAACC CTAGCGCCTC CTCTCACTCC GTATGCGCAG
    ATCATGCAGT CGCTCCAGCT GCCAGGCCAG GCGCAAGGCC CCCCACAGGG CGCAGTCGTA
    TCTCCTACAT CTCGGTCTCC AGCATCTCGG TTGGAACCCA AGCCCCACCC GGCTGTGTTC
    TCCAGGGTGG CCTCCCCACC TCCAGGAGCC TCCGAGAAGC GCCTGCTGCC GGGAGCCGGA
    GCAACCCCAG GGCCAGCCGA AAAAGCCCGG GTCCCGGCGG TCCCCCGCAG GCCAGGGAGC
    AGTCTCAGCA GCAGCATCGA GAACCTGGAG TCGGAGGCCG TGTTCGAGGC CAAGTTCAAG
    CGCAGCCGGG AGTCGCCCCT GTCGCGGGGG CTGCGGCTGC TGAGCCGCTC GCGGTCGGAG
    GAGCGCGGCC CCTTCCGCGG GGCTGAGGAG GAGGACGGCA TTTACCGGCC CAGCCCGGCG
    GGCACGCCGC TGGAGCTGGT GCGACGGCCC GAGCGCTCGC GCTCCGTGCA GGACCTCAGG
    GCGGTCGGGG AGCCAGGCCT CGTGCGCCGC CTCTCGCTGT CGCTGTCCCA GCGTCTGCGG
    CGGACCCCGC CCGCGCAGCG CCACCCGGCC TGGGAGCCCC GCGGCGGGGA CGGCGAGAGC
    TCGGAGGGCG GCAGCTCGGC GCGGGGCTCC CCGGTGCTGA CGGTGCGCCG GAGGCTCAGC
    TCCACGCTGG AGCGGCTGTC GAGCCGGTTG CAGCGCAGCG GCAGCAGCGA GGACTCGGGG
    GGCGCGTCGG GCCGCAGCAC GCCGCTGTTC GGCCGGCTGC GCAGGGCCAC GTCCGAGAGC
    GAGAGTCTGC GGCGCCTGGA CCTCGCGCAA AATCAGCTGG CGGCCCAAGC CGGCGCCGCC
    ACCCCTTCCG CGGAGTCCCT GGGCTCCGAG GCCAGCGCTA CGTCGGGCTC CTCTGCTCCT
    GCAGAAAGTC GAAGCCGCCT GCGCTGGGGC CTCTCTCGGC TGCGGAAGGA CAAGAGGTTG
    TCACAGCCTA ACCTCTCTGG CAGTGTCCAG GAGGATTTGG GTCACCAGTA TGTGCGCAGT
    GAGTCAGACT TTCCCCCAGT CTTCCACATC AAACTCAAGG ACCAGGTGCT GCTGGAGGGA
    GAGGCAGCCA CCCTGCTCTG CTTGCCGGCA GCCTGCCCTG CACCCCACAT CTCCTGGATG
    AAAGACAAGC AGTCCCTGCG GTCAGAGCCC TCGGTGATCA TCGTGTCCTG CAAAGATGGT
    CGTCAGCTAC TGAGCATCCC CCGGGCAGGC AAGCGGCATG CGGGGCTCTA TGAGTGCTCC
    GCCACCAACG TCCTTGGCAG CATCACCAGC TCCTGTACCG TGGCTGTGGC TCGTACCCCA
    GGAAAGCTAG CTCCTCCTGA GGTGCCCCAG ACCTACCAGG ACACAGCACT GGTGCTCTGG
    AAGCCGGGAG ACAGCAAGGC GCCTTGCACA TACACGCTGG AGCGGCGGGT GGATGGGGAG
    TCTTCCTGGC ACCCTGTGAG CTCGGGCATC CCCGACTGCT ACTACAACGT GACCCACCTA
    CCAGTCGGCA TGGCTGTGAG GTTCCGTGTG GCCTGTGCCA ACCGTGCTGG GCAAGGGCCT
    TTCAGCAACC CTTCTGAGAA GGTCCTCGTC AGGGGCGTGC AAGATTCCTC AGCTCTGCCA
    TCTGCTGCTC ACAGAGATGC CCCTGTTACC TCAGGGCCAG CCAGGGCCCC GCCTCCTGAC
    GCTCCTACCT CACTGGCCCC TCCCCTGAGC CCCTCCCCAG CTCCTGCTCC CCCAGGCCCC
    CAGGCAGCCC CTCTCGGCCC TTCATCTTCC CCAGCACCCC CCAGCCAGGC CTTGTCCTCC
    CTCAAGGCTG TGGGTCCACC ACCCCAAACC CCCCCACGAA AGCACCGGGG CCTGCAGGCT
    GCCCAGCAAG TAGAACCTAC CCCACCCAGT GCCCAGGACT CCCCAAGTGA GCCCAAGTCT
    TCCGTCCCTG ACACTGGGAC CCCCATCCCA GCCTCTGCCC CTCAAGTGGT GAAAACAGCC
    TCTTCCTCAA TGCCCCTGTA TATGGTGACC TCCTTTGTGT CTGCACCACC AGCCCCTGAG
    CCCCCAGCCC CCGAGCCCCC TCCTGAGCCC ACCAAAGTGA CTGTGCGGAG CCTCAGCCCA
    GCCAAGGAGG TGGTTGGCTC CCCTGGGGGT GGTCCCCCCA GGGCTCCTAA GCCAGAGGGC
    ACCACTCTTC GACAGGGCCC TCCTCAGAAA CCATACACCT TCCTGGAGGA GAAGGCCAGG
    GGTCGCTTTG GTGTGGTGCG TGCCTGCCGG GAGAATGCCA CCGGGCGAAC ATTCGTGGCC
    AAGATTGTGC CATACGCGGC CGAGGGCAAG CGGCAGGTCC TGCAGGAGTA CGAGGTGCTG
    AGGACCCTGC ACCACGAGCG GCTTATGGCC CTGCACGAAG CCTACATCAC CCCGCGCTAC
    CTCGTGCTCA TCGCCGAGAG CTGCGGCAAC CGGGAGCTTC TCTGTGGGCT CAGCGACAGA
    TTCCGGTATT CAGAGGATGA TGTGGCCACC TATGTGGTGC AGCTGCTACA AGGCCTGGAT
    TACCTCCATG GCCGCCACGT GCTGCACCTG GACATCAAGC CAGACAACCT GCTGCTGGCC
    CCTGACAACG CCCTCAAGAT CGTGGACTTT GGCAGTGCCC AGCCCTACAA CCCCCAGGCT
    CTGCGGCCCC TGGGTCACCG CACGGGCACA CTGGAGTTCA TGGCTCCTGA GATGGTAAAG
    GGAGACCCCA TCGGCTCTGC CACGGACATC TGGGGAGCGG GTGTGCTCAC TTACATCATG
    CTCAGTGGAC GCTCCCCATT CTATGAGCCA GACCCCCAGG AAACAGAGGC TCGCATTGTG
    GGGGGCCGCT TTGATGCCTT CCAGCTGTAT CCCAACACAT CCCAGAGTGC CACCCTCTTC
    TTGCGAAAGG TCCTCTCAGT ACATCCCTGG AGCCGGCCCT CCGTGCAGGA CTGCCTGGCT
    CACCCGTGGC TGCAGGATGC CTACCTGATG AAACTGCGCC GCCAGACACT CACCTTCACC
    ACCAACCGGC TCAAGGAGTT CCTGGGTGAG CAGCGGCGCC GCAGGGCCGA GGCCGCCACC
    CGCCACAAGG TGCTGCTCCG CTCTTACCCA GGCAGCCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87989
    Clone ID Related Accession (Same CDS sequence) XM_015459172.1
    Accession Version XM_015459172.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5052bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product striated muscle preferentially expressed protein kinase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103849.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCGGGCA CGAGGGCACC CCCCAGCCCC 
    GGGGTGCCCC CGAAGAGGGC CAAAGTGGGG GCCGGCGGAG GGGCGGCGGC CGCGGTGGCC
    GGGGCGCCGG TCTTCCTACG GCCCCTGAAG GACGCGGCGG TGTCTGCGGG CAGCGACGTG
    CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCAGCTGGTT CCGGGATGGG
    CAGCGCTTGC CCCCGCCGGC CCCTGAGCCC AGCTGCCTGT GGCTGCGAAG CTGCGGGCCG
    CGGGATGCCG GCGTGTACAG CTGCAGGGCC CAGAACGAGC TGGGCCAGGC CTCCTGCGAG
    GCGGTTCTCA CCGTGCTGGA GGTCGGAGAC TCGGAGACAG GCGAGGATGA CATCAGCGAC
    GGGCAGGGGA CCCAGCGCCT GGAGCTTCGG GATGATGGGG CCTTCAGCAC CCCCACGGGG
    GGCTCTGATA CCCTGGTGGG CACCTCCCTG GACACGCCCC CGACCTCCGT GACAGGCACC
    TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC
    AGCAGGGCTG GCACCCGCCG CCTCCCGGGC AGCCCAAGGC AAGCACAGGC AACCGGGGCC
    GGGCCACGGC ACCTGGGGGT GGAGCCGCTC GTACGGGCAT CTCGAGCTAA TCTGGTGGGC
    GCAAGCTGGG GGTCAGAGGA TAGCCTTTCG GTGGCCAGTG ACCTGTACGG CAGCGCATTC
    AGCCTGTACA GAGGACGGGC GCTCTCGATC CACGTCAGTG TCCCTCAGAG CGGACTGCAC
    AAGGAGGAGC CTGACCTTCA GCCTCAGCCC GCCAGCGAAG CCCCCCGTCG CCCAGCCCTG
    CCGCCCCCCT CCAAATCCGC GCTGCTGCCC CCACCGTCCC CTCGGGTGGG TAAGCGGCCC
    CCGCCGGGAG CCGGCGGCCA GCCCCTGGCC ACCCCCACGC CGCCGCACCG GCGCTCTCGG
    GAGCCGGTGC CGCCCGAGGA CGCCACCGCC GAAGAAAAGC GAGGGAAAAA GTCCAAGTCG
    TCCGGGCCCT CGCTAGCGGG CACGGCGGAG TCCCGGCCCC AGACGCCCCT GAGTGAGGCC
    TCGGGCCGCC AGTCGGCGCT GGGCCGCTCC CCGAGGCTGG TGCGCGCTGG CTCCCGCATC
    CTGGACAAGC TGCAGTTCTT CGAGGAGCGC CGGCGCAGCC TGGAGCGCAG CGACTCGCCG
    CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG CGCAAGGCCC GTTCGCTGGA GCAGCCCAAG
    TCCGAGGGCG GCGGGCCGTG GGGCACGCCC AGGGCCTCGC AGGAGGAGCT GCGGTCCCCG
    GCGGGCAGCG TGGCCGAGCG GCGCCGCCTA TTCCAACAGA AGGCGGCCTC GCTGGACGAG
    CGCACGCGTC AGCGCAGCCC TGCGTCCGAC CTCGAGCTGC GCTTCGCCCA GGAGCTGGGC
    CGCATCCGCC GCTCCACGTC GCGGGAGGAG CTGGTGCGCT CTCACGAGTC CCTGCGCGCC
    ACGCTGCAGC GCGCCCCGTC CCCCCGAGAG CCCGGCGAGC CGCCGCTCTT CTCCCAGCCC
    TCCACCCCCA AGACCCCGCG GGCCTTGAGC CCCGCCGCCG CCCAGCCGCC CCCTGCGAGC
    GTCGCGGAAA AGCCCGGGGA TGAGCCAGGG AGGCCCCGGG GCCGTGGGCC AGCGGGCAGG
    ACGGAGCCGG GGGAAGGCCC GCAGCAGGAG GTTAGGCGCC GGGACCAGTT CCCGCTGACC
    CGGAGCAGAG CCATCCAGGA GTGCCGGAGC CCGGTGCCGC CCCCCACCTC TGAGCCCCCC
    GAGACCAGGA CGAAAGCTCC CCCGGGTCGG AAGCGGGAGC CCCCGCCGCA GGCCGTGCGC
    TTCCTGCCCT GGGCCACGCC GGGCCAGGAG GGCGCTGCTG TGCCCCAGAC CTTGGAGAAG
    AACAGGGCGG GACCCGAGGC CGAGAAGAGG CTGCGCAGAG GGCCAGAGGA GGACGGTCCT
    TGGGGGGCCT GGGACCGCCG AGGGGCCCGC AGCCAGGGCA GAGGTCGCCG GGCGCGGCCC
    ACCTCGCCTG AGCTCGAGTC TTCGGATGAC TCCTATGTGT CTGCTGGAGA AGAGCCTCTG
    GAGGCCCCTG TGTTTGAGAT CCCCCTGCAA AATGCAGTGG TGGCCCCAGG GGTGGACGTG
    CTGCTCAAGT GTATTGTTAC CGCCAACCCC CCGCCCCAAG TGTCCTGGCA GAAGGATGGG
    TCCCCGCTGC GCAGTGATGG CCGCCTTCTC ATCCGGGCAG AAGGCGAGCG GCACACCCTG
    CTGCTCCGGG AGGCCCGGGC CGCCGATGCC GGGAGCTATG CAGCCACTGC CACCAACGAG
    CTGGGCCAGG CCCGCTGCGC TGCCACGCTG GCCGTGAGAC CTGGTGGGTC AACATCTCCT
    TTCAGCAGTC CTATCACCTC TGACGAGGAG TATCTGAGCC CCCCAGAGGA GTTCCCGGAA
    CCCGGGGAGA CCTGGCCCCG AGCCCCCACC ATGAAGCTCA GTCCTGGCCA GAATCGCCGT
    TCCTCCGACA CTGGCTCCAA GGCGCCCCCT ACTTTCAAGG TTTCACTTAT GGACCAGTCA
    GTAAGGGAAG GCCAAGACGT CACCATGAGC ATCCGTGTGC AGGGCGAACC CAAGCCGGTG
    GTCTCCTGGC TGAGAAACCG TCAGCCTGTG CGCCCAGACC AGCGGCGCTT TGCTGAGGAG
    GCAGAGGATG GGCTCTGCCG GCTTCGGATC CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC
    TACACCTGCA AGGCGGTTAA CGAGTATGGC GCTCGACAGT GTGAGGCGCG CCTGGAGGTC
    AGAGCACACC CTGAAAGCCG GTCCCTGGCC GTGCTGGCCC CTCTGCAGGA CGTGGACGTG
    GGGGCCGGGG AGATGGCGCT GTTTGAGTGC CTGGTGGCGG GTCCCACCGA CGTGGAGGTA
    GACTGGCTGT GCCGAGGCCG CCTGCTGCAG CCTGCACTAC TCAAATGCAA GATGCATTTC
    GATGGCCGCA AATGCAAGCT GCTGCTCACC TCTGTGCATG AGGACGACAG CGGCGTCTAC
    ACCTGCAAGC TCAGCACGGC CAAAGATGAA CTGACCTGCA GTGCCCGGCT GACCGTGCGA
    CCCTCGCTGG CACCCCTGTT CACGCGGCTG CTGGAGGACG TGGAAGTGCT GGAGGGCCGC
    GCTGCCCGCT TCGACTGCAA GATCAGCGGG AGCCCGCCCC CCTCCGTTAC CTGGACTCAC
    TTTGGCCACC CCGTGGAGGA GAGCGAGAAC GTGCGGCTGC GGCAGGACGG GGGCCTGCAT
    TCGCTGCACA TCGCACATGT GGGCAGTGAG GATGAGGGGC TGTACGCGGT CAGTGCTGCC
    AACACCCACG GCCAGGCCCA CTGCTCAGCC CAGCTCTACG TGGAGGAGCC TCGGACGGCT
    GCCACCGGCC CTAGCTCCAA GCTGGAGAAG ATGCCCTCCA TCCCCGAGGA GCCCGAGCAG
    GGTGAGCTGG AGCGGCTGTC CATGCCCGAC TTCCTGCGGC CGCTGCAGGA TCTGGACGTG
    GGACTGGCCA AGGAGGCCAT GCTGGAGTGC CAGGTGACCG GCCTGCCCTA CCCCACCATC
    AGCTGGTTCC ACAACGGCCA CCGCATCCAG AGCAGCGACG ACCGACGCAT GACGCAGTAC
    AGGGATGTCC ATCGCTTGGT GTTCCCTGCC GTGGGACCTC AGCATGCTGG TGTCTACAAG
    AGCGTCATTG CTAACAAGCT GGGCAAAGCC GCCTGCTATG CTCATCTCTA CGTCACAGAT
    GTGGTTCCAG GCCCCCCAGA CGGTGCCCCT CAGGTAGTGG CTGTGACCGG GAGGATGATC
    ACGCTTGCAT GGAACCCCCC GAGGAGTCTG GACATGGCTA TCGACCCAGA CGCCCTGACC
    TACACCGTGC AGCACCAGGT GCTGGGCTCA GACCAGTGGA CGGCGCTGGT CACGGGCCTG
    CGGCAGCCCG GGTGGGCAGC CACCGGATTG CGAAAGGGGG TCCAGCACGT CTTCCGGGTT
    CTCAGCACCA CCATCAAGAG CAGCAGCAAG CCCTCGCCCC CGTCCGAGCC CACGCAGCTG
    CTGGAGCGTG GCCCGCCCCT GGAGGAGGCG CCCGCTGTGC TGGACAAGCC AGACGTCGTG
    TATGTGGTGG AGGGCCAGCC CACCAGTGTC ACCGTCACCT TCAACCACGT GGAGGCCCAG
    GTTGTCTGGA GGAGCTGCCG AGGAGCCCTC CTAGAGGCCC GGCCAGGTGT GTTTGAGCTG
    AGCCAGCCAG ATGACGACCA GTACTCTCTT AGGATCTGCC GGGTGAGCCG CCGGGACCTG
    GGAGCCCTCA CTTGCACTGC CCGCAACCGC CACGGCACAC AGGCCTGCTC AGTCACCCTG
    GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC ATCATGGAGG ATGTGGAGGT AGGGGCTGGA
    GAGACTGCTC GCTTCGCCGT GGTGGTGGAC GGGAAGCCCT TGCCGGACAT CATGTGGTAC
    AAGGATGAGG CGCTGCTGAC CGAGAGCAAC CATGTGAGCT TCGTGTACGA GGAGAACGAG
    TGCTCCCTGG TGGTGCTCAG CGCGGGGGCC CAGGATGGAG GGGTCTACAC CTGCACGGCC
    CGTAACCTGG CTGGCGAAGT CTCCTGCAAA GCAGAGCTGG CTGTGCACTC AGCTCAGACA
    GCTATGGAGG TGGAAGGGAT CGGGGAAGAC GAGGAGCAGC GAGGAAGGAG GCTCAGCGAC
    TTCTATGATA TTCAGCAGGA GATCGGCAGG GGTGCCTTCT CCTACCTGCG CCGTGTAGTG
    GAGAGGAGCT CCGGACTGGA GTTTGCAGCC AAGTTCATCC CCAGCCAGGC AAAGCCAAAG
    GCATCGGCAT GGCGGGAGGC ACGGCTGCTG GCCCGGCTCC AGCATGACTG CATCCTCTAC
    TTCCATGAGG CCTTCGAGAG GCGCCGGGGA CTGGTCATCG TCACCGAGCT GGAAGTCCAA
    GACATCTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314658.1
    CDS246..5297
    Translation

    Target ORF information:

    RefSeq Version XM_015459172.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SPEG complex locus (SPEG), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459172.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCGGGCA CGAGGGCACC CCCCAGCCCC 
    GGGGTGCCCC CGAAGAGGGC CAAAGTGGGG GCCGGCGGAG GGGCGGCGGC CGCGGTGGCC
    GGGGCGCCGG TCTTCCTACG GCCCCTGAAG GACGCGGCGG TGTCTGCGGG CAGCGACGTG
    CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCAGCTGGTT CCGGGATGGG
    CAGCGCTTGC CCCCGCCGGC CCCTGAGCCC AGCTGCCTGT GGCTGCGAAG CTGCGGGCCG
    CGGGATGCCG GCGTGTACAG CTGCAGGGCC CAGAACGAGC TGGGCCAGGC CTCCTGCGAG
    GCGGTTCTCA CCGTGCTGGA GGTCGGAGAC TCGGAGACAG GCGAGGATGA CATCAGCGAC
    GGGCAGGGGA CCCAGCGCCT GGAGCTTCGG GATGATGGGG CCTTCAGCAC CCCCACGGGG
    GGCTCTGATA CCCTGGTGGG CACCTCCCTG GACACGCCCC CGACCTCCGT GACAGGCACC
    TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC
    AGCAGGGCTG GCACCCGCCG CCTCCCGGGC AGCCCAAGGC AAGCACAGGC AACCGGGGCC
    GGGCCACGGC ACCTGGGGGT GGAGCCGCTC GTACGGGCAT CTCGAGCTAA TCTGGTGGGC
    GCAAGCTGGG GGTCAGAGGA TAGCCTTTCG GTGGCCAGTG ACCTGTACGG CAGCGCATTC
    AGCCTGTACA GAGGACGGGC GCTCTCGATC CACGTCAGTG TCCCTCAGAG CGGACTGCAC
    AAGGAGGAGC CTGACCTTCA GCCTCAGCCC GCCAGCGAAG CCCCCCGTCG CCCAGCCCTG
    CCGCCCCCCT CCAAATCCGC GCTGCTGCCC CCACCGTCCC CTCGGGTGGG TAAGCGGCCC
    CCGCCGGGAG CCGGCGGCCA GCCCCTGGCC ACCCCCACGC CGCCGCACCG GCGCTCTCGG
    GAGCCGGTGC CGCCCGAGGA CGCCACCGCC GAAGAAAAGC GAGGGAAAAA GTCCAAGTCG
    TCCGGGCCCT CGCTAGCGGG CACGGCGGAG TCCCGGCCCC AGACGCCCCT GAGTGAGGCC
    TCGGGCCGCC AGTCGGCGCT GGGCCGCTCC CCGAGGCTGG TGCGCGCTGG CTCCCGCATC
    CTGGACAAGC TGCAGTTCTT CGAGGAGCGC CGGCGCAGCC TGGAGCGCAG CGACTCGCCG
    CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG CGCAAGGCCC GTTCGCTGGA GCAGCCCAAG
    TCCGAGGGCG GCGGGCCGTG GGGCACGCCC AGGGCCTCGC AGGAGGAGCT GCGGTCCCCG
    GCGGGCAGCG TGGCCGAGCG GCGCCGCCTA TTCCAACAGA AGGCGGCCTC GCTGGACGAG
    CGCACGCGTC AGCGCAGCCC TGCGTCCGAC CTCGAGCTGC GCTTCGCCCA GGAGCTGGGC
    CGCATCCGCC GCTCCACGTC GCGGGAGGAG CTGGTGCGCT CTCACGAGTC CCTGCGCGCC
    ACGCTGCAGC GCGCCCCGTC CCCCCGAGAG CCCGGCGAGC CGCCGCTCTT CTCCCAGCCC
    TCCACCCCCA AGACCCCGCG GGCCTTGAGC CCCGCCGCCG CCCAGCCGCC CCCTGCGAGC
    GTCGCGGAAA AGCCCGGGGA TGAGCCAGGG AGGCCCCGGG GCCGTGGGCC AGCGGGCAGG
    ACGGAGCCGG GGGAAGGCCC GCAGCAGGAG GTTAGGCGCC GGGACCAGTT CCCGCTGACC
    CGGAGCAGAG CCATCCAGGA GTGCCGGAGC CCGGTGCCGC CCCCCACCTC TGAGCCCCCC
    GAGACCAGGA CGAAAGCTCC CCCGGGTCGG AAGCGGGAGC CCCCGCCGCA GGCCGTGCGC
    TTCCTGCCCT GGGCCACGCC GGGCCAGGAG GGCGCTGCTG TGCCCCAGAC CTTGGAGAAG
    AACAGGGCGG GACCCGAGGC CGAGAAGAGG CTGCGCAGAG GGCCAGAGGA GGACGGTCCT
    TGGGGGGCCT GGGACCGCCG AGGGGCCCGC AGCCAGGGCA GAGGTCGCCG GGCGCGGCCC
    ACCTCGCCTG AGCTCGAGTC TTCGGATGAC TCCTATGTGT CTGCTGGAGA AGAGCCTCTG
    GAGGCCCCTG TGTTTGAGAT CCCCCTGCAA AATGCAGTGG TGGCCCCAGG GGTGGACGTG
    CTGCTCAAGT GTATTGTTAC CGCCAACCCC CCGCCCCAAG TGTCCTGGCA GAAGGATGGG
    TCCCCGCTGC GCAGTGATGG CCGCCTTCTC ATCCGGGCAG AAGGCGAGCG GCACACCCTG
    CTGCTCCGGG AGGCCCGGGC CGCCGATGCC GGGAGCTATG CAGCCACTGC CACCAACGAG
    CTGGGCCAGG CCCGCTGCGC TGCCACGCTG GCCGTGAGAC CTGGTGGGTC AACATCTCCT
    TTCAGCAGTC CTATCACCTC TGACGAGGAG TATCTGAGCC CCCCAGAGGA GTTCCCGGAA
    CCCGGGGAGA CCTGGCCCCG AGCCCCCACC ATGAAGCTCA GTCCTGGCCA GAATCGCCGT
    TCCTCCGACA CTGGCTCCAA GGCGCCCCCT ACTTTCAAGG TTTCACTTAT GGACCAGTCA
    GTAAGGGAAG GCCAAGACGT CACCATGAGC ATCCGTGTGC AGGGCGAACC CAAGCCGGTG
    GTCTCCTGGC TGAGAAACCG TCAGCCTGTG CGCCCAGACC AGCGGCGCTT TGCTGAGGAG
    GCAGAGGATG GGCTCTGCCG GCTTCGGATC CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC
    TACACCTGCA AGGCGGTTAA CGAGTATGGC GCTCGACAGT GTGAGGCGCG CCTGGAGGTC
    AGAGCACACC CTGAAAGCCG GTCCCTGGCC GTGCTGGCCC CTCTGCAGGA CGTGGACGTG
    GGGGCCGGGG AGATGGCGCT GTTTGAGTGC CTGGTGGCGG GTCCCACCGA CGTGGAGGTA
    GACTGGCTGT GCCGAGGCCG CCTGCTGCAG CCTGCACTAC TCAAATGCAA GATGCATTTC
    GATGGCCGCA AATGCAAGCT GCTGCTCACC TCTGTGCATG AGGACGACAG CGGCGTCTAC
    ACCTGCAAGC TCAGCACGGC CAAAGATGAA CTGACCTGCA GTGCCCGGCT GACCGTGCGA
    CCCTCGCTGG CACCCCTGTT CACGCGGCTG CTGGAGGACG TGGAAGTGCT GGAGGGCCGC
    GCTGCCCGCT TCGACTGCAA GATCAGCGGG AGCCCGCCCC CCTCCGTTAC CTGGACTCAC
    TTTGGCCACC CCGTGGAGGA GAGCGAGAAC GTGCGGCTGC GGCAGGACGG GGGCCTGCAT
    TCGCTGCACA TCGCACATGT GGGCAGTGAG GATGAGGGGC TGTACGCGGT CAGTGCTGCC
    AACACCCACG GCCAGGCCCA CTGCTCAGCC CAGCTCTACG TGGAGGAGCC TCGGACGGCT
    GCCACCGGCC CTAGCTCCAA GCTGGAGAAG ATGCCCTCCA TCCCCGAGGA GCCCGAGCAG
    GGTGAGCTGG AGCGGCTGTC CATGCCCGAC TTCCTGCGGC CGCTGCAGGA TCTGGACGTG
    GGACTGGCCA AGGAGGCCAT GCTGGAGTGC CAGGTGACCG GCCTGCCCTA CCCCACCATC
    AGCTGGTTCC ACAACGGCCA CCGCATCCAG AGCAGCGACG ACCGACGCAT GACGCAGTAC
    AGGGATGTCC ATCGCTTGGT GTTCCCTGCC GTGGGACCTC AGCATGCTGG TGTCTACAAG
    AGCGTCATTG CTAACAAGCT GGGCAAAGCC GCCTGCTATG CTCATCTCTA CGTCACAGAT
    GTGGTTCCAG GCCCCCCAGA CGGTGCCCCT CAGGTAGTGG CTGTGACCGG GAGGATGATC
    ACGCTTGCAT GGAACCCCCC GAGGAGTCTG GACATGGCTA TCGACCCAGA CGCCCTGACC
    TACACCGTGC AGCACCAGGT GCTGGGCTCA GACCAGTGGA CGGCGCTGGT CACGGGCCTG
    CGGCAGCCCG GGTGGGCAGC CACCGGATTG CGAAAGGGGG TCCAGCACGT CTTCCGGGTT
    CTCAGCACCA CCATCAAGAG CAGCAGCAAG CCCTCGCCCC CGTCCGAGCC CACGCAGCTG
    CTGGAGCGTG GCCCGCCCCT GGAGGAGGCG CCCGCTGTGC TGGACAAGCC AGACGTCGTG
    TATGTGGTGG AGGGCCAGCC CACCAGTGTC ACCGTCACCT TCAACCACGT GGAGGCCCAG
    GTTGTCTGGA GGAGCTGCCG AGGAGCCCTC CTAGAGGCCC GGCCAGGTGT GTTTGAGCTG
    AGCCAGCCAG ATGACGACCA GTACTCTCTT AGGATCTGCC GGGTGAGCCG CCGGGACCTG
    GGAGCCCTCA CTTGCACTGC CCGCAACCGC CACGGCACAC AGGCCTGCTC AGTCACCCTG
    GAGCTGGCAG AGGCCCCTCG GTTTGAGTCC ATCATGGAGG ATGTGGAGGT AGGGGCTGGA
    GAGACTGCTC GCTTCGCCGT GGTGGTGGAC GGGAAGCCCT TGCCGGACAT CATGTGGTAC
    AAGGATGAGG CGCTGCTGAC CGAGAGCAAC CATGTGAGCT TCGTGTACGA GGAGAACGAG
    TGCTCCCTGG TGGTGCTCAG CGCGGGGGCC CAGGATGGAG GGGTCTACAC CTGCACGGCC
    CGTAACCTGG CTGGCGAAGT CTCCTGCAAA GCAGAGCTGG CTGTGCACTC AGCTCAGACA
    GCTATGGAGG TGGAAGGGAT CGGGGAAGAC GAGGAGCAGC GAGGAAGGAG GCTCAGCGAC
    TTCTATGATA TTCAGCAGGA GATCGGCAGG GGTGCCTTCT CCTACCTGCG CCGTGTAGTG
    GAGAGGAGCT CCGGACTGGA GTTTGCAGCC AAGTTCATCC CCAGCCAGGC AAAGCCAAAG
    GCATCGGCAT GGCGGGAGGC ACGGCTGCTG GCCCGGCTCC AGCATGACTG CATCCTCTAC
    TTCCATGAGG CCTTCGAGAG GCGCCGGGGA CTGGTCATCG TCACCGAGCT GGAAGTCCAA
    GACATCTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87990
    Clone ID Related Accession (Same CDS sequence) XM_015459173.1
    Accession Version XM_015459173.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product striated muscle preferentially expressed protein kinase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103849.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCGGGCA CGAGGGCACC CCCCAGCCCC 
    GGGGTGCCCC CGAAGAGGGC CAAAGTGGGG GCCGGCGGAG GGGCGGCGGC CGCGGTGGCC
    GGGGCGCCGG TCTTCCTACG GCCCCTGAAG GACGCGGCGG TGTCTGCGGG CAGCGACGTG
    CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCAGCTGGTT CCGGGATGGG
    CAGCGCTTGC CCCCGCCGGC CCCTGAGCCC AGCTGCCTGT GGCTGCGAAG CTGCGGGCCG
    CGGGATGCCG GCGTGTACAG CTGCAGGGCC CAGAACGAGC TGGGCCAGGC CTCCTGCGAG
    GCGGTTCTCA CCGTGCTGGA GGTCGGAGAC TCGGAGACAG GCGAGGATGA CATCAGCGAC
    GGGCAGGGGA CCCAGCGCCT GGAGCTTCGG GATGATGGGG CCTTCAGCAC CCCCACGGGG
    GGCTCTGATA CCCTGGTGGG CACCTCCCTG GACACGCCCC CGACCTCCGT GACAGGCACC
    TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC
    AGCAGGGCTG GCACCCGCCG CCTCCCGGGC AGCCCAAGGC AAGCACAGGC AACCGGGGCC
    GGGCCACGGC ACCTGGGGGT GGAGCCGCTC GTACGGGCAT CTCGAGCTAA TCTGGTGGGC
    GCAAGCTGGG GGTCAGAGGA TAGCCTTTCG GTGGCCAGTG ACCTGTACGG CAGCGCATTC
    AGCCTGTACA GAGGACGGGC GCTCTCGATC CACGTCAGTG TCCCTCAGAG CGGACTGCAC
    AAGGAGGAGC CTGACCTTCA GCCTCAGCCC GCCAGCGAAG CCCCCCGTCG CCCAGCCCTG
    CCGCCCCCCT CCAAATCCGC GCTGCTGCCC CCACCGTCCC CTCGGGTGGG TAAGCGGCCC
    CCGCCGGGAG CCGGCGGCCA GCCCCTGGCC ACCCCCACGC CGCCGCACCG GCGCTCTCGG
    GAGCCGGTGC CGCCCGAGGA CGCCACCGCC GAAGAAAAGC GAGGGAAAAA GTCCAAGTCG
    TCCGGGCCCT CGCTAGCGGG CACGGCGGAG TCCCGGCCCC AGACGCCCCT GAGTGAGGCC
    TCGGGCCGCC AGTCGGCGCT GGGCCGCTCC CCGAGGCTGG TGCGCGCTGG CTCCCGCATC
    CTGGACAAGC TGCAGTTCTT CGAGGAGCGC CGGCGCAGCC TGGAGCGCAG CGACTCGCCG
    CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG CGCAAGGCCC GTTCGCTGGA GCAGCCCAAG
    TCCGAGGGCG GCGGGCCGTG GGGCACGCCC AGGGCCTCGC AGGAGGAGCT GCGGTCCCCG
    GCGGGCAGCG TGGCCGAGCG GCGCCGCCTA TTCCAACAGA AGGCGGCCTC GCTGGACGAG
    CGCACGCGTC AGCGCAGCCC TGCGTCCGAC CTCGAGCTGC GCTTCGCCCA GGAGCTGGGC
    CGCATCCGCC GCTCCACGTC GCGGGAGGAG CTGGTGCGCT CTCACGAGTC CCTGCGCGCC
    ACGCTGCAGC GCGCCCCGTC CCCCCGAGAG CCCGGCGAGC CGCCGCTCTT CTCCCAGCCC
    TCCACCCCCA AGACCCCGCG GGCCTTGAGC CCCGCCGCCG CCCAGCCGCC CCCTGCGAGC
    GTCGCGGAAA AGCCCGGGGA TGAGCCAGGG AGGCCCCGGG GCCGTGGGCC AGCGGGCAGG
    ACGGAGCCGG GGGAAGGCCC GCAGCAGGAG GTTAGGCGCC GGGACCAGTT CCCGCTGACC
    CGGAGCAGAG CCATCCAGGA GTGCCGGAGC CCGGTGCCGC CCCCCACCTC TGAGCCCCCC
    GAGACCAGGA CGAAAGCTCC CCCGGGTCGG AAGCGGGAGC CCCCGCCGCA GGCCGTGCGC
    TTCCTGCCCT GGGCCACGCC GGGCCAGGAG GGCGCTGCTG TGCCCCAGAC CTTGGAGAAG
    AACAGGGCGG GACCCGAGGC CGAGAAGAGG CTGCGCAGAG GGCCAGAGGA GGACGGTCCT
    TGGGGGGCCT GGGACCGCCG AGGGGCCCGC AGCCAGGGCA GAGGTCGCCG GGCGCGGCCC
    ACCTCGCCTG AGCTCGAGTC TTCGGATGAC TCCTATGTGT CTGCTGGAGA AGAGCCTCTG
    GAGGCCCCTG TGTTTGAGAT CCCCCTGCAA AATGCAGTGG TGGCCCCAGG GGTGGACGTG
    CTGCTCAAGT GTATTGTTAC CGCCAACCCC CCGCCCCAAG TGTCCTGGCA GAAGGATGGG
    TCCCCGCTGC GCAGTGATGG CCGCCTTCTC ATCCGGGCAG AAGGCGAGCG GCACACCCTG
    CTGCTCCGGG AGGCCCGGGC CGCCGATGCC GGGAGCTATG CAGCCACTGC CACCAACGAG
    CTGGGCCAGG CCCGCTGCGC TGCCACGCTG GCCGTGAGAC CTGGTGGGTC AACATCTCCT
    TTCAGCAGTC CTATCACCTC TGACGAGGAG TATCTGAGCC CCCCAGAGGA GTTCCCGGAA
    CCCGGGGAGA CCTGGCCCCG AGCCCCCACC ATGAAGCTCA GTCCTGGCCA GAATCGCCGT
    TCCTCCGACA CTGGCTCCAA GGCGCCCCCT ACTTTCAAGG TTTCACTTAT GGACCAGTCA
    GTAAGGGAAG GCCAAGACGT CACCATGAGC ATCCGTGTGC AGGGCGAACC CAAGCCGGTG
    GTCTCCTGGC TGAGAAACCG TCAGCCTGTG CGCCCAGACC AGCGGCGCTT TGCTGAGGAG
    GCAGAGGATG GGCTCTGCCG GCTTCGGATC CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC
    TACACCTGCA AGGCGGTTAA CGAGTATGGC GCTCGACAGT GTGAGGCGCG CCTGGAGGTC
    AGAGGACGTG GACGTGGGGG CCGGGGAGAT GGCGCTGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314659.1
    CDS254..3175
    Translation

    Target ORF information:

    RefSeq Version XM_015459173.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SPEG complex locus (SPEG), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459173.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCGGGCA CGAGGGCACC CCCCAGCCCC 
    GGGGTGCCCC CGAAGAGGGC CAAAGTGGGG GCCGGCGGAG GGGCGGCGGC CGCGGTGGCC
    GGGGCGCCGG TCTTCCTACG GCCCCTGAAG GACGCGGCGG TGTCTGCGGG CAGCGACGTG
    CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCAGCTGGTT CCGGGATGGG
    CAGCGCTTGC CCCCGCCGGC CCCTGAGCCC AGCTGCCTGT GGCTGCGAAG CTGCGGGCCG
    CGGGATGCCG GCGTGTACAG CTGCAGGGCC CAGAACGAGC TGGGCCAGGC CTCCTGCGAG
    GCGGTTCTCA CCGTGCTGGA GGTCGGAGAC TCGGAGACAG GCGAGGATGA CATCAGCGAC
    GGGCAGGGGA CCCAGCGCCT GGAGCTTCGG GATGATGGGG CCTTCAGCAC CCCCACGGGG
    GGCTCTGATA CCCTGGTGGG CACCTCCCTG GACACGCCCC CGACCTCCGT GACAGGCACC
    TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC
    AGCAGGGCTG GCACCCGCCG CCTCCCGGGC AGCCCAAGGC AAGCACAGGC AACCGGGGCC
    GGGCCACGGC ACCTGGGGGT GGAGCCGCTC GTACGGGCAT CTCGAGCTAA TCTGGTGGGC
    GCAAGCTGGG GGTCAGAGGA TAGCCTTTCG GTGGCCAGTG ACCTGTACGG CAGCGCATTC
    AGCCTGTACA GAGGACGGGC GCTCTCGATC CACGTCAGTG TCCCTCAGAG CGGACTGCAC
    AAGGAGGAGC CTGACCTTCA GCCTCAGCCC GCCAGCGAAG CCCCCCGTCG CCCAGCCCTG
    CCGCCCCCCT CCAAATCCGC GCTGCTGCCC CCACCGTCCC CTCGGGTGGG TAAGCGGCCC
    CCGCCGGGAG CCGGCGGCCA GCCCCTGGCC ACCCCCACGC CGCCGCACCG GCGCTCTCGG
    GAGCCGGTGC CGCCCGAGGA CGCCACCGCC GAAGAAAAGC GAGGGAAAAA GTCCAAGTCG
    TCCGGGCCCT CGCTAGCGGG CACGGCGGAG TCCCGGCCCC AGACGCCCCT GAGTGAGGCC
    TCGGGCCGCC AGTCGGCGCT GGGCCGCTCC CCGAGGCTGG TGCGCGCTGG CTCCCGCATC
    CTGGACAAGC TGCAGTTCTT CGAGGAGCGC CGGCGCAGCC TGGAGCGCAG CGACTCGCCG
    CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG CGCAAGGCCC GTTCGCTGGA GCAGCCCAAG
    TCCGAGGGCG GCGGGCCGTG GGGCACGCCC AGGGCCTCGC AGGAGGAGCT GCGGTCCCCG
    GCGGGCAGCG TGGCCGAGCG GCGCCGCCTA TTCCAACAGA AGGCGGCCTC GCTGGACGAG
    CGCACGCGTC AGCGCAGCCC TGCGTCCGAC CTCGAGCTGC GCTTCGCCCA GGAGCTGGGC
    CGCATCCGCC GCTCCACGTC GCGGGAGGAG CTGGTGCGCT CTCACGAGTC CCTGCGCGCC
    ACGCTGCAGC GCGCCCCGTC CCCCCGAGAG CCCGGCGAGC CGCCGCTCTT CTCCCAGCCC
    TCCACCCCCA AGACCCCGCG GGCCTTGAGC CCCGCCGCCG CCCAGCCGCC CCCTGCGAGC
    GTCGCGGAAA AGCCCGGGGA TGAGCCAGGG AGGCCCCGGG GCCGTGGGCC AGCGGGCAGG
    ACGGAGCCGG GGGAAGGCCC GCAGCAGGAG GTTAGGCGCC GGGACCAGTT CCCGCTGACC
    CGGAGCAGAG CCATCCAGGA GTGCCGGAGC CCGGTGCCGC CCCCCACCTC TGAGCCCCCC
    GAGACCAGGA CGAAAGCTCC CCCGGGTCGG AAGCGGGAGC CCCCGCCGCA GGCCGTGCGC
    TTCCTGCCCT GGGCCACGCC GGGCCAGGAG GGCGCTGCTG TGCCCCAGAC CTTGGAGAAG
    AACAGGGCGG GACCCGAGGC CGAGAAGAGG CTGCGCAGAG GGCCAGAGGA GGACGGTCCT
    TGGGGGGCCT GGGACCGCCG AGGGGCCCGC AGCCAGGGCA GAGGTCGCCG GGCGCGGCCC
    ACCTCGCCTG AGCTCGAGTC TTCGGATGAC TCCTATGTGT CTGCTGGAGA AGAGCCTCTG
    GAGGCCCCTG TGTTTGAGAT CCCCCTGCAA AATGCAGTGG TGGCCCCAGG GGTGGACGTG
    CTGCTCAAGT GTATTGTTAC CGCCAACCCC CCGCCCCAAG TGTCCTGGCA GAAGGATGGG
    TCCCCGCTGC GCAGTGATGG CCGCCTTCTC ATCCGGGCAG AAGGCGAGCG GCACACCCTG
    CTGCTCCGGG AGGCCCGGGC CGCCGATGCC GGGAGCTATG CAGCCACTGC CACCAACGAG
    CTGGGCCAGG CCCGCTGCGC TGCCACGCTG GCCGTGAGAC CTGGTGGGTC AACATCTCCT
    TTCAGCAGTC CTATCACCTC TGACGAGGAG TATCTGAGCC CCCCAGAGGA GTTCCCGGAA
    CCCGGGGAGA CCTGGCCCCG AGCCCCCACC ATGAAGCTCA GTCCTGGCCA GAATCGCCGT
    TCCTCCGACA CTGGCTCCAA GGCGCCCCCT ACTTTCAAGG TTTCACTTAT GGACCAGTCA
    GTAAGGGAAG GCCAAGACGT CACCATGAGC ATCCGTGTGC AGGGCGAACC CAAGCCGGTG
    GTCTCCTGGC TGAGAAACCG TCAGCCTGTG CGCCCAGACC AGCGGCGCTT TGCTGAGGAG
    GCAGAGGATG GGCTCTGCCG GCTTCGGATC CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC
    TACACCTGCA AGGCGGTTAA CGAGTATGGC GCTCGACAGT GTGAGGCGCG CCTGGAGGTC
    AGAGGACGTG GACGTGGGGG CCGGGGAGAT GGCGCTGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87991
    Clone ID Related Accession (Same CDS sequence) XM_015459174.1
    Accession Version XM_015459174.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2892bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product striated muscle preferentially expressed protein kinase isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103849.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCGGGCA CGAGGGCACC CCCCAGCCCC 
    GGGGTGCCCC CGAAGAGGGC CAAAGTGGGG GCCGGCGGAG GGGCGGCGGC CGCGGTGGCC
    GGGGCGCCGG TCTTCCTACG GCCCCTGAAG GACGCGGCGG TGTCTGCGGG CAGCGACGTG
    CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCAGCTGGTT CCGGGATGGG
    CAGCGCTTGC CCCCGCCGGC CCCTGAGCCC AGCTGCCTGT GGCTGCGAAG CTGCGGGCCG
    CGGGATGCCG GCGTGTACAG CTGCAGGGCC CAGAACGAGC TGGGCCAGGC CTCCTGCGAG
    GCGGTTCTCA CCGTGCTGGA GGTCGGAGAC TCGGAGACAG GCGAGGATGA CATCAGCGAC
    GGGCAGGGGA CCCAGCGCCT GGAGCTTCGG GATGATGGGG CCTTCAGCAC CCCCACGGGG
    GGCTCTGATA CCCTGGTGGG CACCTCCCTG GACACGCCCC CGACCTCCGT GACAGGCACC
    TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC
    AGCAGGGCTG GCACCCGCCG CCTCCCGGGC AGCCCAAGGC AAGCACAGGC AACCGGGGCC
    GGGCCACGGC ACCTGGGGGT GGAGCCGCTC GTACGGGCAT CTCGAGCTAA TCTGGTGGGC
    GCAAGCTGGG GGTCAGAGGA TAGCCTTTCG GTGGCCAGTG ACCTGTACGG CAGCGCATTC
    AGCCTGTACA GAGGACGGGC GCTCTCGATC CACGTCAGTG TCCCTCAGAG CGGACTGCAC
    AAGGAGGAGC CTGACCTTCA GCCTCAGCCC GCCAGCGAAG CCCCCCGTCG CCCAGCCCTG
    CCGCCCCCCT CCAAATCCGC GCTGCTGCCC CCACCGTCCC CTCGGGTGGG TAAGCGGCCC
    CCGCCGGGAG CCGGCGGCCA GCCCCTGGCC ACCCCCACGC CGCCGCACCG GCGCTCTCGG
    GAGCCGGTGC CGCCCGAGGA CGCCACCGCC GAAGAAAAGC GAGGGAAAAA GTCCAAGTCG
    TCCGGGCCCT CGCTAGCGGG CACGGCGGAG TCCCGGCCCC AGACGCCCCT GAGTGAGGCC
    TCGGGCCGCC AGTCGGCGCT GGGCCGCTCC CCGAGGCTGG TGCGCGCTGG CTCCCGCATC
    CTGGACAAGC TGCAGTTCTT CGAGGAGCGC CGGCGCAGCC TGGAGCGCAG CGACTCGCCG
    CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG CGCAAGGCCC GTTCGCTGGA GCAGCCCAAG
    TCCGAGGGCG GCGGGCCGTG GGGCACGCCC AGGGCCTCGC AGGAGGAGCT GCGGTCCCCG
    GCGGGCAGCG TGGCCGAGCG GCGCCGCCTA TTCCAACAGA AGGCGGCCTC GCTGGACGAG
    CGCACGCGTC AGCGCAGCCC TGCGTCCGAC CTCGAGCTGC GCTTCGCCCA GGAGCTGGGC
    CGCATCCGCC GCTCCACGTC GCGGGAGGAG CTGGTGCGCT CTCACGAGTC CCTGCGCGCC
    ACGCTGCAGC GCGCCCCGTC CCCCCGAGAG CCCGGCGAGC CGCCGCTCTT CTCCCAGCCC
    TCCACCCCCA AGACCCCGCG GGCCTTGAGC CCCGCCGCCG CCCAGCCGCC CCCTGCGAGC
    GTCGCGGAAA AGCCCGGGGA TGAGCCAGGG AGGCCCCGGG GCCGTGGGCC AGCGGGCAGG
    ACGGAGCCGG GGGAAGGCCC GCAGCAGGAG GTTAGGCGCC GGGACCAGTT CCCGCTGACC
    CGGAGCAGAG CCATCCAGGA GTGCCGGAGC CCGGTGCCGC CCCCCACCTC TGAGCCCCCC
    GAGACCAGGA CGAAAGCTCC CCCGGGTCGG AAGCGGGAGC CCCCGCCGCA GGCCGTGCGC
    TTCCTGCCCT GGGCCACGCC GGGCCAGGAG GGCGCTGCTG TGCCCCAGAC CTTGGAGAAG
    AACAGGGCGG GACCCGAGGC CGAGAAGAGG CTGCGCAGAG GGCCAGAGGA GGACGGTCCT
    TGGGGGGCCT GGGACCGCCG AGGGGCCCGC AGCCAGGGCA GAGGTCGCCG GGCGCGGCCC
    ACCTCGCCTG AGCTCGAGTC TTCGGATGAC TCCTATGTGT CTGCTGGAGA AGAGCCTCTG
    GAGGCCCCTG TGTTTGAGAT CCCCCTGCAA AATGCAGTGG TGGCCCCAGG GGTGGACGTG
    CTGCTCAAGT GTATTGTTAC CGCCAACCCC CCGCCCCAAG TGTCCTGGCA GAAGGATGGG
    TCCCCGCTGC GCAGTGATGG CCGCCTTCTC ATCCGGGCAG AAGGCGAGCG GCACACCCTG
    CTGCTCCGGG AGGCCCGGGC CGCCGATGCC GGGAGCTATG CAGCCACTGC CACCAACGAG
    CTGGGCCAGG CCCGCTGCGC TGCCACGCTG GCCGTGAGAC CTGGTGGGTC AACATCTCCT
    TTCAGCAGTC CTATCACCTC TGACGAGGAG TATCTGAGCC CCCCAGAGGA GTTCCCGGAA
    CCCGGGGAGA CCTGGCCCCG AGCCCCCACC ATGAAGCTCA GTCCTGGCCA GAATCGCCGT
    TCCTCCGACA CTGGCTCCAA GGCGCCCCCT ACTTTCAAGG TTTCACTTAT GGACCAGTCA
    GTAAGGGAAG GCCAAGACGT CACCATGAGC ATCCGTGTGC AGGGCGAACC CAAGCCGGTG
    GTCTCCTGGC TGAGAAACCG TCAGCCTGTG CGCCCAGACC AGCGGCGCTT TGCTGAGGAG
    GCAGAGGATG GGCTCTGCCG GCTTCGGATC CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC
    TACACCTGCA AGGCGGTTAA CGAGTATGGC GCTCGACAGT GTGAGGCGCG CCTGGAGGTC
    AGAGGCGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314660.1
    CDS253..3144
    Translation

    Target ORF information:

    RefSeq Version XM_015459174.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SPEG complex locus (SPEG), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459174.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCAGAAAG CCCGGGGCAC GCGAGGCGAG GACGCGGGCA CGAGGGCACC CCCCAGCCCC 
    GGGGTGCCCC CGAAGAGGGC CAAAGTGGGG GCCGGCGGAG GGGCGGCGGC CGCGGTGGCC
    GGGGCGCCGG TCTTCCTACG GCCCCTGAAG GACGCGGCGG TGTCTGCGGG CAGCGACGTG
    CGGCTGCGGG TGGTGGTGAG CGGGACGCCC CAGCCCAGCC TCAGCTGGTT CCGGGATGGG
    CAGCGCTTGC CCCCGCCGGC CCCTGAGCCC AGCTGCCTGT GGCTGCGAAG CTGCGGGCCG
    CGGGATGCCG GCGTGTACAG CTGCAGGGCC CAGAACGAGC TGGGCCAGGC CTCCTGCGAG
    GCGGTTCTCA CCGTGCTGGA GGTCGGAGAC TCGGAGACAG GCGAGGATGA CATCAGCGAC
    GGGCAGGGGA CCCAGCGCCT GGAGCTTCGG GATGATGGGG CCTTCAGCAC CCCCACGGGG
    GGCTCTGATA CCCTGGTGGG CACCTCCCTG GACACGCCCC CGACCTCCGT GACAGGCACC
    TCAGAGGAGC AAGTGAGCTG GTGGGGCAGC GGGCAGACGG TCCTGGAGCA GGAAGCGGGC
    AGCAGGGCTG GCACCCGCCG CCTCCCGGGC AGCCCAAGGC AAGCACAGGC AACCGGGGCC
    GGGCCACGGC ACCTGGGGGT GGAGCCGCTC GTACGGGCAT CTCGAGCTAA TCTGGTGGGC
    GCAAGCTGGG GGTCAGAGGA TAGCCTTTCG GTGGCCAGTG ACCTGTACGG CAGCGCATTC
    AGCCTGTACA GAGGACGGGC GCTCTCGATC CACGTCAGTG TCCCTCAGAG CGGACTGCAC
    AAGGAGGAGC CTGACCTTCA GCCTCAGCCC GCCAGCGAAG CCCCCCGTCG CCCAGCCCTG
    CCGCCCCCCT CCAAATCCGC GCTGCTGCCC CCACCGTCCC CTCGGGTGGG TAAGCGGCCC
    CCGCCGGGAG CCGGCGGCCA GCCCCTGGCC ACCCCCACGC CGCCGCACCG GCGCTCTCGG
    GAGCCGGTGC CGCCCGAGGA CGCCACCGCC GAAGAAAAGC GAGGGAAAAA GTCCAAGTCG
    TCCGGGCCCT CGCTAGCGGG CACGGCGGAG TCCCGGCCCC AGACGCCCCT GAGTGAGGCC
    TCGGGCCGCC AGTCGGCGCT GGGCCGCTCC CCGAGGCTGG TGCGCGCTGG CTCCCGCATC
    CTGGACAAGC TGCAGTTCTT CGAGGAGCGC CGGCGCAGCC TGGAGCGCAG CGACTCGCCG
    CCGGCGCCCC TGCGGCCCTG GGTGCCCCTG CGCAAGGCCC GTTCGCTGGA GCAGCCCAAG
    TCCGAGGGCG GCGGGCCGTG GGGCACGCCC AGGGCCTCGC AGGAGGAGCT GCGGTCCCCG
    GCGGGCAGCG TGGCCGAGCG GCGCCGCCTA TTCCAACAGA AGGCGGCCTC GCTGGACGAG
    CGCACGCGTC AGCGCAGCCC TGCGTCCGAC CTCGAGCTGC GCTTCGCCCA GGAGCTGGGC
    CGCATCCGCC GCTCCACGTC GCGGGAGGAG CTGGTGCGCT CTCACGAGTC CCTGCGCGCC
    ACGCTGCAGC GCGCCCCGTC CCCCCGAGAG CCCGGCGAGC CGCCGCTCTT CTCCCAGCCC
    TCCACCCCCA AGACCCCGCG GGCCTTGAGC CCCGCCGCCG CCCAGCCGCC CCCTGCGAGC
    GTCGCGGAAA AGCCCGGGGA TGAGCCAGGG AGGCCCCGGG GCCGTGGGCC AGCGGGCAGG
    ACGGAGCCGG GGGAAGGCCC GCAGCAGGAG GTTAGGCGCC GGGACCAGTT CCCGCTGACC
    CGGAGCAGAG CCATCCAGGA GTGCCGGAGC CCGGTGCCGC CCCCCACCTC TGAGCCCCCC
    GAGACCAGGA CGAAAGCTCC CCCGGGTCGG AAGCGGGAGC CCCCGCCGCA GGCCGTGCGC
    TTCCTGCCCT GGGCCACGCC GGGCCAGGAG GGCGCTGCTG TGCCCCAGAC CTTGGAGAAG
    AACAGGGCGG GACCCGAGGC CGAGAAGAGG CTGCGCAGAG GGCCAGAGGA GGACGGTCCT
    TGGGGGGCCT GGGACCGCCG AGGGGCCCGC AGCCAGGGCA GAGGTCGCCG GGCGCGGCCC
    ACCTCGCCTG AGCTCGAGTC TTCGGATGAC TCCTATGTGT CTGCTGGAGA AGAGCCTCTG
    GAGGCCCCTG TGTTTGAGAT CCCCCTGCAA AATGCAGTGG TGGCCCCAGG GGTGGACGTG
    CTGCTCAAGT GTATTGTTAC CGCCAACCCC CCGCCCCAAG TGTCCTGGCA GAAGGATGGG
    TCCCCGCTGC GCAGTGATGG CCGCCTTCTC ATCCGGGCAG AAGGCGAGCG GCACACCCTG
    CTGCTCCGGG AGGCCCGGGC CGCCGATGCC GGGAGCTATG CAGCCACTGC CACCAACGAG
    CTGGGCCAGG CCCGCTGCGC TGCCACGCTG GCCGTGAGAC CTGGTGGGTC AACATCTCCT
    TTCAGCAGTC CTATCACCTC TGACGAGGAG TATCTGAGCC CCCCAGAGGA GTTCCCGGAA
    CCCGGGGAGA CCTGGCCCCG AGCCCCCACC ATGAAGCTCA GTCCTGGCCA GAATCGCCGT
    TCCTCCGACA CTGGCTCCAA GGCGCCCCCT ACTTTCAAGG TTTCACTTAT GGACCAGTCA
    GTAAGGGAAG GCCAAGACGT CACCATGAGC ATCCGTGTGC AGGGCGAACC CAAGCCGGTG
    GTCTCCTGGC TGAGAAACCG TCAGCCTGTG CGCCCAGACC AGCGGCGCTT TGCTGAGGAG
    GCAGAGGATG GGCTCTGCCG GCTTCGGATC CTGGCTGCCG AGCGGGGCGA CGCTGGCTTC
    TACACCTGCA AGGCGGTTAA CGAGTATGGC GCTCGACAGT GTGAGGCGCG CCTGGAGGTC
    AGAGGCGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.