FEM1B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FEM1B
Entrez Gene ID	10116
Full Name	fem-1 homolog B
Synonyms	F1A-ALPHA,F1AA,FEM1-beta
General protein information	Preferred Names fem-1 homolog B Names protein fem-1 homolog B FEM-1-like death receptor binding protein fem-1-like death receptor-binding protein alpha fem-1-like in apoptotic pathway protein alpha
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q23
Summary	This gene encodes an ankyrin repeat protein that belongs to the death receptor-associated family of proteins and plays a role in mediating apoptosis. The encoded protein is also thought to function in the replication stress-induced checkpoint signaling pathway via interaction with checkpoint kinase 1. [provided by RefSeq, Aug 2013].
Disorder MIM:	613539

mRNA and Protein(s)

mRNA	Protein	Name
NM_015322.4	NP_056137.1	protein fem-1 homolog B
XM_017021859.1	XP_016877348.1	protein fem-1 homolog B isoform X1
NM_015322.5	NP_056137.1	protein fem-1 homolog B

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	FEM1B	NP_001025724.1
Danio rerio (zebrafish)	fem1b	XP_695502.4
Xenopus tropicalis (tropical clawed frog)	fem1b	NP_001238770.1
Homo sapiens (human)	FEM1B	NP_056137.1
Pan troglodytes (chimpanzee)	FEM1B	XP_523110.2
Bos taurus (cattle)	FEM1B	NP_001179312.1
Mus musculus (house mouse)	Fem1b	NP_034323.1
Rattus norvegicus (Norway rat)	Fem1b	NP_001101627.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007382	XP_308449.4
	FEM1B	XP_001083413.1
Canis lupus familiaris (dog)	FEM1B	XP_535526.3
Drosophila melanogaster (fruit fly)	Fem-1	NP_611508.1

Gene Ontology
Bibliography

Related articles in PubMed

FEM1 proteins are ancient regulators of SLBP degradation.
Dankert JF, Pagan JK, Starostina NG, Kipreos ET, Pagano M
Cell cycle (Georgetown, Tex.)16(6)556-564(2017 Mar)

Fem1b promotes ubiquitylation and suppresses transcriptional activity of Gli1.
Gilder AS, Chen YB, Jackson RJ 3rd, Jiang J, Maher JF
Biochemical and biophysical research communications440(3)431-6(2013 Oct)

Family-based analysis of candidate genes for polycystic ovary syndrome.
Ewens KG, Stewart DR, Ankener W, Urbanek M, McAllister JM, Chen C, Baig KM, Parker SC, Margulies EH, Legro RS, Dunaif A, Strauss JF 3rd, Spielman RS
The Journal of clinical endocrinology and metabolism95(5)2306-15(2010 May)

Fem1b, a proapoptotic protein, mediates proteasome inhibitor-induced apoptosis of human colon cancer cells.
Subauste MC, Sansom OJ, Porecha N, Raich N, Du L, Maher JF
Molecular carcinogenesis49(2)105-13(2010 Feb)

Human FEM1B is required for Rad9 recruitment and CHK1 activation in response to replication stress.
Sun TP, Shieh SY
Oncogene28(18)1971-81(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FEM1 proteins are ancient regulators of Stem-Loop Binding Protein.
Title: FEM1 proteins are ancient regulators of SLBP degradation.

These findings have implications for understanding the cellular functions of Fem1b, and the regulation of Gli1 oncoprotein activity.
Title: Fem1b promotes ubiquitylation and suppresses transcriptional activity of Gli1.

Observational study of gene-disease association. (HuGE Navigator)
Title: Family-based analysis of candidate genes for polycystic ovary syndrome.

the proapoptotic protein Fem1b is downregulated by the proteasome in malignant colon cancer cells and mediates proteasome inhibitor-induced apoptosis of these cells
Title: Fem1b, a proapoptotic protein, mediates proteasome inhibitor-induced apoptosis of human colon cancer cells.

Observational study of gene-disease association, gene-gene interaction, gene-environment interaction, and genetic testing. (HuGE Navigator)
Title: Integrative predictive model of coronary artery calcification in atherosclerosis.

The following FEM1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FEM1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21504	NM_015322.5 Latest version!	Homo sapiens fem-1 homolog B (FEM1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu85224	XM_017021859.1 Latest version!	Homo sapiens fem-1 homolog B (FEM1B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu21504	NM_015322.4	Homo sapiens fem-1 homolog B (FEM1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FEM1B ( NM_015322.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056137.1 Homo sapiens fem-1 homolog B (FEM1B), mRNA.

Price & Availability↑

CloneID	OHu21504
Clone ID Related Accession (Same CDS sequence)	NM_015322.4 , NM_015322.5
Accession Version	NM_015322.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	protein fem-1 homolog B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC010122.2, AC021553.14, AB007856.2 and BF112021.1. On Aug 21, 2013 this sequence version replaced NM_015322.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC010122.2, SRR1660807.20143.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGCC TGGCTGGCTA TGTATACAAG GCGGCCAGCG AGGGCAAGGT GCTGACTCTG 
GCCGCCTTGC TTCTCAACCG GTCTGAAAGC GACATCCGCT ATCTGCTTGG CTATGTCAGC 
CAGCAGGGAG GGCAGCGCTC CACGCCCCTC ATCATCGCAG CCCGCAATGG ACACGCAAAG 
GTGGTACGCT TGCTCTTAGA ACATTACCGG GTGCAGACTC AGCAGACTGG CACCGTCCGC 
TTCGACGGGT ATGTCATTGA TGGTGCCACT GCTCTTTGGT GTGCAGCTGG AGCAGGACAT 
TTTGAAGTTG TTAAACTTCT AGTCAGCCAT GGAGCCAACG TGAACCATAC CACAGTAACT 
AATTCAACCC CCCTGCGGGC AGCATGCTTT GATGGCAGAC TGGACATTGT GAAATACTTG 
GTTGAAAATA ATGCCAACAT CAGCATTGCC AACAAATATG ACAACACCTG CCTAATGATT 
GCGGCATATA AGGGACACAC TGATGTGGTC AGATACCTTT TAGAACAACG TGCTGATCCC 
AATGCCAAAG CACATTGTGG AGCCACAGCA TTGCACTTTG CAGCTGAAGC TGGGCACATA 
GATATTGTGA AAGAGCTGAT AAAATGGCGT GCTGCTATAG TAGTGAATGG CCATGGGATG 
ACGCCATTGA AAGTAGCTGC CGAAAGCTGT AAAGCTGATG TCGTAGAACT GTTACTCTCT 
CATGCTGATT GCGACCGAAG AAGTCGGATT GAAGCTTTGG AACTCTTGGG TGCCTCCTTT 
GCAAATGACC GTGAGAACTA TGACATCATA AAGACATACC ACTATCTATA TTTAGCCATG 
TTAGAGAGGT TCCAAGATGG TGATAACATT CTCGAAAAAG AGGTTCTTCC ACCAATCCAT 
GCTTATGGGA ATAGAACTGA ATGTAGAAAT CCTCAGGAAC TGGAGTCCAT TCGGCAAGAC 
AGAGATGCTC TTCATATGGA AGGCCTTATA GTTCGGGAAC GGATTTTAGG TGCTGACAAT 
ATTGATGTTT CTCATCCCAT CATTTACAGA GGAGCTGTTT ATGCGGATAA TATGGAATTT 
GAGCAGTGTA TCAAGTTGTG GCTTCATGCC CTGCACCTCA GACAAAAAGG TAACAGGAAC 
ACCCACAAGG ATCTTCTTCG ATTTGCTCAA GTTTTCTCAC AAATGATACA TTTGAATGAA 
ACTGTGAAGG CCCCAGACAT AGAATGTGTT TTGAGATGCA GTGTTTTGGA AATAGAACAA 
AGTATGAACA GAGTGAAAAA TATTTCAGAT GCTGATGTCC ACAATGCTAT GGACAATTAT 
GAATGTAATC TCTATACCTT TCTGTATTTA GTGTGCATCT CTACCAAAAC ACAGTGCAGC 
GAAGAAGATC AGTGCAAAAT TAACAAGCAG ATCTACAACC TGATTCACCT TGATCCCAGA 
ACTCGTGAAG GTTTCACCTT GCTGCATCTG GCTGTCAATT CCAATACTCC AGTTGATGAT 
TTCCACACCA ATGACGTCTG CAGCTTTCCA AATGCACTTG TCACAAAGCT CCTGCTGGAC 
TGTGGTGCTG AGGTGAATGC CGTGGACAAT GAGGGAAACA GTGCCCTTCA TATTATCGTT 
CAGTACAACA GGCCCATCAG TGATTTTTTG ACCTTGCACT CCATCATCAT TAGCCTAGTT 
GAAGCCGGAG CTCACACTGA CATGACGAAT AAACAGAATA AGACTCCGCT AGACAAAAGT 
ACAACTGGGG TATCTGAAAT ACTGCTTAAA ACTCAAATGA AGATGAGTCT CAAGTGCCTG 
GCTGCCCGAG CAGTTCGGGC TAATGACATT AACTACCAAG ACCAGATCCC CAGAACTCTT 
GAAGAGTTTG TTGGATTTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056137.1
CDS	616..2499
Translation	MEGLAGYVYKAASEGKVLTLAALLLNRSESDIRYLLGYVSQQGGQRSTPLIIAARNGHAKVVRLLLEHYRVQTQQTGTVRFDGYVIDGATALWCAAGAGHFEVVKLLVSHGANVNHTTVTNSTPLRAACFDGRLDIVKYLVENNANISIANKYDNTCLMIAAYKGHTDVVRYLLEQRADPNAKAHCGATALHFAAEAGHIDIVKELIKWRAAIVVNGHGMTPLKVAAESCKADVVELLLSHADCDRRSRIEALELLGASFANDRENYDIIKTYHYLYLAMLERFQDGDNILEKEVLPPIHAYGNRTECRNPQELESIRQDRDALHMEGLIVRERILGADNIDVSHPIIYRGAVYADNMEFEQCIKLWLHALHLRQKGNRNTHKDLLRFAQVFSQMIHLNETVKAPDIECVLRCSVLEIEQSMNRVKNISDADVHNAMDNYECNLYTFLYLVCISTKTQCSEEDQCKINKQIYNLIHLDPRTREGFTLLHLAVNSNTPVDDFHTNDVCSFPNALVTKLLLDCGAEVNAVDNEGNSALHIIVQYNRPISDFLTLHSIIISLVEAGAHTDMTNKQNKTPLDKSTTGVSEILLKTQMKMSLKCLAARAVRANDINYQDQIPRTLEEFVGFH

Target ORF information:

RefSeq Version	NM_015322.4
Organism	Homo sapiens(human)
Definition	Homo sapiens fem-1 homolog B (FEM1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015322.4

ORF Insert Sequence:

ATGGAGGGCC TGGCTGGCTA TGTATACAAG GCGGCCAGCG AGGGCAAGGT GCTGACTCTG 
GCCGCCTTGC TTCTCAACCG GTCTGAAAGC GACATCCGCT ATCTGCTTGG CTATGTCAGC 
CAGCAGGGAG GGCAGCGCTC CACGCCCCTC ATCATCGCAG CCCGCAATGG ACACGCAAAG 
GTGGTACGCT TGCTCTTAGA ACATTACCGG GTGCAGACTC AGCAGACTGG CACCGTCCGC 
TTCGACGGGT ATGTCATTGA TGGTGCCACT GCTCTTTGGT GTGCAGCTGG AGCAGGACAT 
TTTGAAGTTG TTAAACTTCT AGTCAGCCAT GGAGCCAACG TGAACCATAC CACAGTAACT 
AATTCAACCC CCCTGCGGGC AGCATGCTTT GATGGCAGAC TGGACATTGT GAAATACTTG 
GTTGAAAATA ATGCCAACAT CAGCATTGCC AACAAATATG ACAACACCTG CCTAATGATT 
GCGGCATATA AGGGACACAC TGATGTGGTC AGATACCTTT TAGAACAACG TGCTGATCCC 
AATGCCAAAG CACATTGTGG AGCCACAGCA TTGCACTTTG CAGCTGAAGC TGGGCACATA 
GATATTGTGA AAGAGCTGAT AAAATGGCGT GCTGCTATAG TAGTGAATGG CCATGGGATG 
ACGCCATTGA AAGTAGCTGC CGAAAGCTGT AAAGCTGATG TCGTAGAACT GTTACTCTCT 
CATGCTGATT GCGACCGAAG AAGTCGGATT GAAGCTTTGG AACTCTTGGG TGCCTCCTTT 
GCAAATGACC GTGAGAACTA TGACATCATA AAGACATACC ACTATCTATA TTTAGCCATG 
TTAGAGAGGT TCCAAGATGG TGATAACATT CTCGAAAAAG AGGTTCTTCC ACCAATCCAT 
GCTTATGGGA ATAGAACTGA ATGTAGAAAT CCTCAGGAAC TGGAGTCCAT TCGGCAAGAC 
AGAGATGCTC TTCATATGGA AGGCCTTATA GTTCGGGAAC GGATTTTAGG TGCTGACAAT 
ATTGATGTTT CTCATCCCAT CATTTACAGA GGAGCTGTTT ATGCGGATAA TATGGAATTT 
GAGCAGTGTA TCAAGTTGTG GCTTCATGCC CTGCACCTCA GACAAAAAGG TAACAGGAAC 
ACCCACAAGG ATCTTCTTCG ATTTGCTCAA GTTTTCTCAC AAATGATACA TTTGAATGAA 
ACTGTGAAGG CCCCAGACAT AGAATGTGTT TTGAGATGCA GTGTTTTGGA AATAGAACAA 
AGTATGAACA GAGTGAAAAA TATTTCAGAT GCTGATGTCC ACAATGCTAT GGACAATTAT 
GAATGTAATC TCTATACCTT TCTGTATTTA GTGTGCATCT CTACCAAAAC ACAGTGCAGC 
GAAGAAGATC AGTGCAAAAT TAACAAGCAG ATCTACAACC TGATTCACCT TGATCCCAGA 
ACTCGTGAAG GTTTCACCTT GCTGCATCTG GCTGTCAATT CCAATACTCC AGTTGATGAT 
TTCCACACCA ATGACGTCTG CAGCTTTCCA AATGCACTTG TCACAAAGCT CCTGCTGGAC 
TGTGGTGCTG AGGTGAATGC CGTGGACAAT GAGGGAAACA GTGCCCTTCA TATTATCGTT 
CAGTACAACA GGCCCATCAG TGATTTTTTG ACCTTGCACT CCATCATCAT TAGCCTAGTT 
GAAGCCGGAG CTCACACTGA CATGACGAAT AAACAGAATA AGACTCCGCT AGACAAAAGT 
ACAACTGGGG TATCTGAAAT ACTGCTTAAA ACTCAAATGA AGATGAGTCT CAAGTGCCTG 
GCTGCCCGAG CAGTTCGGGC TAATGACATT AACTACCAAG ACCAGATCCC CAGAACTCTT 
GAAGAGTTTG TTGGATTTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FEM1B ( NM_015322.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_056137.1 Homo sapiens fem-1 homolog B (FEM1B), mRNA.

Price & Availability↑

CloneID	OHu21504
Clone ID Related Accession (Same CDS sequence)	NM_015322.4 , NM_015322.5
Accession Version	NM_015322.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	protein fem-1 homolog B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107871.10, BC010122.2, AC021553.14, AB007856.2 and BF112021.1. On Nov 22, 2018 this sequence version replaced NM_015322.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.169560.1, SRR1660807.20143.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000306917.5/ ENSP00000307298.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGGCC TGGCTGGCTA TGTATACAAG GCGGCCAGCG AGGGCAAGGT GCTGACTCTG 
GCCGCCTTGC TTCTCAACCG GTCTGAAAGC GACATCCGCT ATCTGCTTGG CTATGTCAGC 
CAGCAGGGAG GGCAGCGCTC CACGCCCCTC ATCATCGCAG CCCGCAATGG ACACGCAAAG 
GTGGTACGCT TGCTCTTAGA ACATTACCGG GTGCAGACTC AGCAGACTGG CACCGTCCGC 
TTCGACGGGT ATGTCATTGA TGGTGCCACT GCTCTTTGGT GTGCAGCTGG AGCAGGACAT 
TTTGAAGTTG TTAAACTTCT AGTCAGCCAT GGAGCCAACG TGAACCATAC CACAGTAACT 
AATTCAACCC CCCTGCGGGC AGCATGCTTT GATGGCAGAC TGGACATTGT GAAATACTTG 
GTTGAAAATA ATGCCAACAT CAGCATTGCC AACAAATATG ACAACACCTG CCTAATGATT 
GCGGCATATA AGGGACACAC TGATGTGGTC AGATACCTTT TAGAACAACG TGCTGATCCC 
AATGCCAAAG CACATTGTGG AGCCACAGCA TTGCACTTTG CAGCTGAAGC TGGGCACATA 
GATATTGTGA AAGAGCTGAT AAAATGGCGT GCTGCTATAG TAGTGAATGG CCATGGGATG 
ACGCCATTGA AAGTAGCTGC CGAAAGCTGT AAAGCTGATG TCGTAGAACT GTTACTCTCT 
CATGCTGATT GCGACCGAAG AAGTCGGATT GAAGCTTTGG AACTCTTGGG TGCCTCCTTT 
GCAAATGACC GTGAGAACTA TGACATCATA AAGACATACC ACTATCTATA TTTAGCCATG 
TTAGAGAGGT TCCAAGATGG TGATAACATT CTCGAAAAAG AGGTTCTTCC ACCAATCCAT 
GCTTATGGGA ATAGAACTGA ATGTAGAAAT CCTCAGGAAC TGGAGTCCAT TCGGCAAGAC 
AGAGATGCTC TTCATATGGA AGGCCTTATA GTTCGGGAAC GGATTTTAGG TGCTGACAAT 
ATTGATGTTT CTCATCCCAT CATTTACAGA GGAGCTGTTT ATGCGGATAA TATGGAATTT 
GAGCAGTGTA TCAAGTTGTG GCTTCATGCC CTGCACCTCA GACAAAAAGG TAACAGGAAC 
ACCCACAAGG ATCTTCTTCG ATTTGCTCAA GTTTTCTCAC AAATGATACA TTTGAATGAA 
ACTGTGAAGG CCCCAGACAT AGAATGTGTT TTGAGATGCA GTGTTTTGGA AATAGAACAA 
AGTATGAACA GAGTGAAAAA TATTTCAGAT GCTGATGTCC ACAATGCTAT GGACAATTAT 
GAATGTAATC TCTATACCTT TCTGTATTTA GTGTGCATCT CTACCAAAAC ACAGTGCAGC 
GAAGAAGATC AGTGCAAAAT TAACAAGCAG ATCTACAACC TGATTCACCT TGATCCCAGA 
ACTCGTGAAG GTTTCACCTT GCTGCATCTG GCTGTCAATT CCAATACTCC AGTTGATGAT 
TTCCACACCA ATGACGTCTG CAGCTTTCCA AATGCACTTG TCACAAAGCT CCTGCTGGAC 
TGTGGTGCTG AGGTGAATGC CGTGGACAAT GAGGGAAACA GTGCCCTTCA TATTATCGTT 
CAGTACAACA GGCCCATCAG TGATTTTTTG ACCTTGCACT CCATCATCAT TAGCCTAGTT 
GAAGCCGGAG CTCACACTGA CATGACGAAT AAACAGAATA AGACTCCGCT AGACAAAAGT 
ACAACTGGGG TATCTGAAAT ACTGCTTAAA ACTCAAATGA AGATGAGTCT CAAGTGCCTG 
GCTGCCCGAG CAGTTCGGGC TAATGACATT AACTACCAAG ACCAGATCCC CAGAACTCTT 
GAAGAGTTTG TTGGATTTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056137.1
CDS	674..2557
Translation	MEGLAGYVYKAASEGKVLTLAALLLNRSESDIRYLLGYVSQQGGQRSTPLIIAARNGHAKVVRLLLEHYRVQTQQTGTVRFDGYVIDGATALWCAAGAGHFEVVKLLVSHGANVNHTTVTNSTPLRAACFDGRLDIVKYLVENNANISIANKYDNTCLMIAAYKGHTDVVRYLLEQRADPNAKAHCGATALHFAAEAGHIDIVKELIKWRAAIVVNGHGMTPLKVAAESCKADVVELLLSHADCDRRSRIEALELLGASFANDRENYDIIKTYHYLYLAMLERFQDGDNILEKEVLPPIHAYGNRTECRNPQELESIRQDRDALHMEGLIVRERILGADNIDVSHPIIYRGAVYADNMEFEQCIKLWLHALHLRQKGNRNTHKDLLRFAQVFSQMIHLNETVKAPDIECVLRCSVLEIEQSMNRVKNISDADVHNAMDNYECNLYTFLYLVCISTKTQCSEEDQCKINKQIYNLIHLDPRTREGFTLLHLAVNSNTPVDDFHTNDVCSFPNALVTKLLLDCGAEVNAVDNEGNSALHIIVQYNRPISDFLTLHSIIISLVEAGAHTDMTNKQNKTPLDKSTTGVSEILLKTQMKMSLKCLAARAVRANDINYQDQIPRTLEEFVGFH

Target ORF information:

RefSeq Version	NM_015322.5
Organism	Homo sapiens(human)
Definition	Homo sapiens fem-1 homolog B (FEM1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015322.5

ORF Insert Sequence:

ATGGAGGGCC TGGCTGGCTA TGTATACAAG GCGGCCAGCG AGGGCAAGGT GCTGACTCTG 
GCCGCCTTGC TTCTCAACCG GTCTGAAAGC GACATCCGCT ATCTGCTTGG CTATGTCAGC 
CAGCAGGGAG GGCAGCGCTC CACGCCCCTC ATCATCGCAG CCCGCAATGG ACACGCAAAG 
GTGGTACGCT TGCTCTTAGA ACATTACCGG GTGCAGACTC AGCAGACTGG CACCGTCCGC 
TTCGACGGGT ATGTCATTGA TGGTGCCACT GCTCTTTGGT GTGCAGCTGG AGCAGGACAT 
TTTGAAGTTG TTAAACTTCT AGTCAGCCAT GGAGCCAACG TGAACCATAC CACAGTAACT 
AATTCAACCC CCCTGCGGGC AGCATGCTTT GATGGCAGAC TGGACATTGT GAAATACTTG 
GTTGAAAATA ATGCCAACAT CAGCATTGCC AACAAATATG ACAACACCTG CCTAATGATT 
GCGGCATATA AGGGACACAC TGATGTGGTC AGATACCTTT TAGAACAACG TGCTGATCCC 
AATGCCAAAG CACATTGTGG AGCCACAGCA TTGCACTTTG CAGCTGAAGC TGGGCACATA 
GATATTGTGA AAGAGCTGAT AAAATGGCGT GCTGCTATAG TAGTGAATGG CCATGGGATG 
ACGCCATTGA AAGTAGCTGC CGAAAGCTGT AAAGCTGATG TCGTAGAACT GTTACTCTCT 
CATGCTGATT GCGACCGAAG AAGTCGGATT GAAGCTTTGG AACTCTTGGG TGCCTCCTTT 
GCAAATGACC GTGAGAACTA TGACATCATA AAGACATACC ACTATCTATA TTTAGCCATG 
TTAGAGAGGT TCCAAGATGG TGATAACATT CTCGAAAAAG AGGTTCTTCC ACCAATCCAT 
GCTTATGGGA ATAGAACTGA ATGTAGAAAT CCTCAGGAAC TGGAGTCCAT TCGGCAAGAC 
AGAGATGCTC TTCATATGGA AGGCCTTATA GTTCGGGAAC GGATTTTAGG TGCTGACAAT 
ATTGATGTTT CTCATCCCAT CATTTACAGA GGAGCTGTTT ATGCGGATAA TATGGAATTT 
GAGCAGTGTA TCAAGTTGTG GCTTCATGCC CTGCACCTCA GACAAAAAGG TAACAGGAAC 
ACCCACAAGG ATCTTCTTCG ATTTGCTCAA GTTTTCTCAC AAATGATACA TTTGAATGAA 
ACTGTGAAGG CCCCAGACAT AGAATGTGTT TTGAGATGCA GTGTTTTGGA AATAGAACAA 
AGTATGAACA GAGTGAAAAA TATTTCAGAT GCTGATGTCC ACAATGCTAT GGACAATTAT 
GAATGTAATC TCTATACCTT TCTGTATTTA GTGTGCATCT CTACCAAAAC ACAGTGCAGC 
GAAGAAGATC AGTGCAAAAT TAACAAGCAG ATCTACAACC TGATTCACCT TGATCCCAGA 
ACTCGTGAAG GTTTCACCTT GCTGCATCTG GCTGTCAATT CCAATACTCC AGTTGATGAT 
TTCCACACCA ATGACGTCTG CAGCTTTCCA AATGCACTTG TCACAAAGCT CCTGCTGGAC 
TGTGGTGCTG AGGTGAATGC CGTGGACAAT GAGGGAAACA GTGCCCTTCA TATTATCGTT 
CAGTACAACA GGCCCATCAG TGATTTTTTG ACCTTGCACT CCATCATCAT TAGCCTAGTT 
GAAGCCGGAG CTCACACTGA CATGACGAAT AAACAGAATA AGACTCCGCT AGACAAAAGT 
ACAACTGGGG TATCTGAAAT ACTGCTTAAA ACTCAAATGA AGATGAGTCT CAAGTGCCTG 
GCTGCCCGAG CAGTTCGGGC TAATGACATT AACTACCAAG ACCAGATCCC CAGAACTCTT 
GAAGAGTTTG TTGGATTTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FEM1B ( XM_017021859.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877348.1 Homo sapiens fem-1 homolog B (FEM1B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu85224
Clone ID Related Accession (Same CDS sequence)	XM_017021859.1
Accession Version	XM_017021859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein fem-1 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCGG CATATAAGGG ACACACTGAT GTGGTCAGAT ACCTTTTAGA ACAACGTGCT 
GATCCCAATG CCAAAGCACA TTGTGGAGCC ACAGCATTGC ACTTTGCAGC TGAAGCTGGG 
CACATAGATA TTGTGAAAGA GCTGATAAAA TGGCGTGCTG CTATAGTAGT GAATGGCCAT 
GGGATGACGC CATTGAAAGT AGCTGCCGAA AGCTGTAAAG CTGATGTCGT AGAACTGTTA 
CTCTCTCATG CTGATTGCGA CCGAAGAAGT CGGATTGAAG CTTTGGAACT CTTGGGTGCC 
TCCTTTGCAA ATGACCGTGA GAACTATGAC ATCATAAAGA CATACCACTA TCTATATTTA 
GCCATGTTAG AGAGGTTCCA AGATGGTGAT AACATTCTCG AAAAAGAGGT TCTTCCACCA 
ATCCATGCTT ATGGGAATAG AACTGAATGT AGAAATCCTC AGGAACTGGA GTCCATTCGG 
CAAGACAGAG ATGCTCTTCA TATGGAAGGC CTTATAGTTC GGGAACGGAT TTTAGGTGCT 
GACAATATTG ATGTTTCTCA TCCCATCATT TACAGAGGAG CTGTTTATGC GGATAATATG 
GAATTTGAGC AGTGTATCAA GTTGTGGCTT CATGCCCTGC ACCTCAGACA AAAAGGTAAC 
AGGAACACCC ACAAGGATCT TCTTCGATTT GCTCAAGTTT TCTCACAAAT GATACATTTG 
AATGAAACTG TGAAGGCCCC AGACATAGAA TGTGTTTTGA GATGCAGTGT TTTGGAAATA 
GAACAAAGTA TGAACAGAGT GAAAAATATT TCAGATGCTG ATGTCCACAA TGCTATGGAC 
AATTATGAAT GTAATCTCTA TACCTTTCTG TATTTAGTGT GCATCTCTAC CAAAACACAG 
TGCAGCGAAG AAGATCAGTG CAAAATTAAC AAGCAGATCT ACAACCTGAT TCACCTTGAT 
CCCAGAACTC GTGAAGGTTT CACCTTGCTG CATCTGGCTG TCAATTCCAA TACTCCAGTT 
GATGATTTCC ACACCAATGA CGTCTGCAGC TTTCCAAATG CACTTGTCAC AAAGCTCCTG 
CTGGACTGTG GTGCTGAGGT GAATGCCGTG GACAATGAGG GAAACAGTGC CCTTCATATT 
ATCGTTCAGT ACAACAGGCC CATCAGTGAT TTTTTGACCT TGCACTCCAT CATCATTAGC 
CTAGTTGAAG CCGGAGCTCA CACTGACATG ACGAATAAAC AGAATAAGAC TCCGCTAGAC 
AAAAGTACAA CTGGGGTATC TGAAATACTG CTTAAAACTC AAATGAAGAT GAGTCTCAAG 
TGCCTGGCTG CCCGAGCAGT TCGGGCTAAT GACATTAACT ACCAAGACCA GATCCCCAGA 
ACTCTTGAAG AGTTTGTTGG ATTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877348.1
CDS	603..2012
Translation	MIAAYKGHTDVVRYLLEQRADPNAKAHCGATALHFAAEAGHIDIVKELIKWRAAIVVNGHGMTPLKVAAESCKADVVELLLSHADCDRRSRIEALELLGASFANDRENYDIIKTYHYLYLAMLERFQDGDNILEKEVLPPIHAYGNRTECRNPQELESIRQDRDALHMEGLIVRERILGADNIDVSHPIIYRGAVYADNMEFEQCIKLWLHALHLRQKGNRNTHKDLLRFAQVFSQMIHLNETVKAPDIECVLRCSVLEIEQSMNRVKNISDADVHNAMDNYECNLYTFLYLVCISTKTQCSEEDQCKINKQIYNLIHLDPRTREGFTLLHLAVNSNTPVDDFHTNDVCSFPNALVTKLLLDCGAEVNAVDNEGNSALHIIVQYNRPISDFLTLHSIIISLVEAGAHTDMTNKQNKTPLDKSTTGVSEILLKTQMKMSLKCLAARAVRANDINYQDQIPRTLEEFVGFH

Target ORF information:

RefSeq Version	XM_017021859.1
Organism	Homo sapiens(human)
Definition	Homo sapiens fem-1 homolog B (FEM1B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021859.1

ORF Insert Sequence:

ATGATTGCGG CATATAAGGG ACACACTGAT GTGGTCAGAT ACCTTTTAGA ACAACGTGCT 
GATCCCAATG CCAAAGCACA TTGTGGAGCC ACAGCATTGC ACTTTGCAGC TGAAGCTGGG 
CACATAGATA TTGTGAAAGA GCTGATAAAA TGGCGTGCTG CTATAGTAGT GAATGGCCAT 
GGGATGACGC CATTGAAAGT AGCTGCCGAA AGCTGTAAAG CTGATGTCGT AGAACTGTTA 
CTCTCTCATG CTGATTGCGA CCGAAGAAGT CGGATTGAAG CTTTGGAACT CTTGGGTGCC 
TCCTTTGCAA ATGACCGTGA GAACTATGAC ATCATAAAGA CATACCACTA TCTATATTTA 
GCCATGTTAG AGAGGTTCCA AGATGGTGAT AACATTCTCG AAAAAGAGGT TCTTCCACCA 
ATCCATGCTT ATGGGAATAG AACTGAATGT AGAAATCCTC AGGAACTGGA GTCCATTCGG 
CAAGACAGAG ATGCTCTTCA TATGGAAGGC CTTATAGTTC GGGAACGGAT TTTAGGTGCT 
GACAATATTG ATGTTTCTCA TCCCATCATT TACAGAGGAG CTGTTTATGC GGATAATATG 
GAATTTGAGC AGTGTATCAA GTTGTGGCTT CATGCCCTGC ACCTCAGACA AAAAGGTAAC 
AGGAACACCC ACAAGGATCT TCTTCGATTT GCTCAAGTTT TCTCACAAAT GATACATTTG 
AATGAAACTG TGAAGGCCCC AGACATAGAA TGTGTTTTGA GATGCAGTGT TTTGGAAATA 
GAACAAAGTA TGAACAGAGT GAAAAATATT TCAGATGCTG ATGTCCACAA TGCTATGGAC 
AATTATGAAT GTAATCTCTA TACCTTTCTG TATTTAGTGT GCATCTCTAC CAAAACACAG 
TGCAGCGAAG AAGATCAGTG CAAAATTAAC AAGCAGATCT ACAACCTGAT TCACCTTGAT 
CCCAGAACTC GTGAAGGTTT CACCTTGCTG CATCTGGCTG TCAATTCCAA TACTCCAGTT 
GATGATTTCC ACACCAATGA CGTCTGCAGC TTTCCAAATG CACTTGTCAC AAAGCTCCTG 
CTGGACTGTG GTGCTGAGGT GAATGCCGTG GACAATGAGG GAAACAGTGC CCTTCATATT 
ATCGTTCAGT ACAACAGGCC CATCAGTGAT TTTTTGACCT TGCACTCCAT CATCATTAGC 
CTAGTTGAAG CCGGAGCTCA CACTGACATG ACGAATAAAC AGAATAAGAC TCCGCTAGAC 
AAAAGTACAA CTGGGGTATC TGAAATACTG CTTAAAACTC AAATGAAGAT GAGTCTCAAG 
TGCCTGGCTG CCCGAGCAGT TCGGGCTAAT GACATTAACT ACCAAGACCA GATCCCCAGA 
ACTCTTGAAG AGTTTGTTGG ATTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FEM1 proteins are ancient regulators of SLBP degradation.
Cell cycle (Georgetown, Tex.)16(6)556-564(2017 Mar)
Dankert JF,Pagan JK,Starostina NG,Kipreos ET,Pagano M

Fem1b promotes ubiquitylation and suppresses transcriptional activity of Gli1.
Biochemical and biophysical research communications440(3)431-6(2013 Oct)
Gilder AS,Chen YB,Jackson RJ 3rd,Jiang J,Maher JF

Family-based analysis of candidate genes for polycystic ovary syndrome.
The Journal of clinical endocrinology and metabolism95(5)2306-15(2010 May)
Ewens KG,Stewart DR,Ankener W,Urbanek M,McAllister JM,Chen C,Baig KM,Parker SC,Margulies EH,Legro RS,Dunaif A,Strauss JF 3rd,Spielman RS

Fem1b, a proapoptotic protein, mediates proteasome inhibitor-induced apoptosis of human colon cancer cells.
Molecular carcinogenesis49(2)105-13(2010 Feb)
Subauste MC,Sansom OJ,Porecha N,Raich N,Du L,Maher JF

Human FEM1B is required for Rad9 recruitment and CHK1 activation in response to replication stress.
Oncogene28(18)1971-81(2009 May)
Sun TP,Shieh SY