Fem-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fem-1
Entrez Gene ID	37344
Full Name	CG9025 gene product from transcript CG9025-RA
Synonyms	CG9025,Dmel\CG9025,dFEM-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 57A9-57A9

mRNA and Protein(s)

mRNA	Protein	Name
NM_166408.2	NP_725993.1	Fem-1, isoform B
NM_137664.3	NP_611508.1	Fem-1, isoform A

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	FEM1B	NP_001025724.1
Danio rerio (zebrafish)	fem1b	XP_695502.4
Xenopus tropicalis (tropical clawed frog)	fem1b	NP_001238770.1
Homo sapiens (human)	FEM1B	NP_056137.1
Pan troglodytes (chimpanzee)	FEM1B	XP_523110.2
Bos taurus (cattle)	FEM1B	NP_001179312.1
Mus musculus (house mouse)	Fem1b	NP_034323.1
Rattus norvegicus (Norway rat)	Fem1b	NP_001101627.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007382	XP_308449.4
	FEM1B	XP_001083413.1
Canis lupus familiaris (dog)	FEM1B	XP_535526.3
Drosophila melanogaster (fruit fly)	Fem-1	NP_611508.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Fem-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fem-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10701	NM_166408.2 Latest version!	Drosophila melanogaster Fem-1, transcript variant B (Fem-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa10700	NM_137664.3 Latest version!	Drosophila melanogaster Fem-1, transcript variant A (Fem-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fem-1 ( NM_166408.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725993.1 Drosophila melanogaster Fem-1, transcript variant B (Fem-1), mRNA.

Price & Availability↑

CloneID	OFa10701
Clone ID Related Accession (Same CDS sequence)	NM_166408.2
Accession Version	NM_166408.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Fem-1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166408.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACGCGG AGCTGTATCG CACTCGCAAG CGGTGGTATG AGTCCGCTTG GCCTGGAGCA 
GGATTCTGGC GAGGAGCCAA CGACACGCTG CCCGTGACGA ACTACATCAC GTATCCGGCG 
AATTTCGATC AGCCGGATGG ACAATCGCTA ACACCCCTGA CCATGGCCGC GATGATGGGC 
AATGTGAAAT TCGTGAAGAC TCTGCTGTCG CACTACGACG TCGACTTGGA CCGAGAATGC 
AATGTCATCT ACGACGGGAT GGTGGTCTAC GGAGCCACAG CCCTGTGGGT GGCTGCCGGA 
ATGGGGCATC TGCAGATAGT CAAAATGCTC GTCCAAGCGG GAGCGGCCAT CAATCACAAT 
ACCAAGGCGC AGTCGTCGCC GCTGAGAGCG GCCTGCTATG AGGGTCGATT GGACATCGTT 
GAATTCCTAA TTGACAATGG CGCTGATGTG AATGCCACGA ATTTGTTCAA CAACAACACG 
CTTATGATTG CCGCCTACAA GGGTCACCAC CTGGTGGTAA AGACTCTACT TCAGAATGGA 
TCAAGGGCCA ATGATCAGGC GCTGTGCGGC GCCACTGCCT TGCACTATGC GGCCGAAAGC 
GGTCACCTGG ATGTGGTGGT AGCCCTGCTT GACCATGGCG CCACTCTGAA GAAGAATGAG 
CTGGGAATTA CGCCCGCCTT GCAGGCTGCC GAACGTCTCC ATGAGGATGT CCTTGAAGCG 
TTCATCGAAC GACCAGAACT GATGAGCTTG GAGGAGCAGA TCACAGCATT GGAGCTTCTG 
GGAGCCACTT ATGCGAATGA CAAAATCAAA TACGACGTGA ATAAGGCCTA TGGCTATCTG 
CTGAGAGCCA TGCAGCTACG TTACAGCAAT CCACGCGGTG TGATCCGCAA AAGAGTTCAG 
CCCACTGTGC CCGCGTATGA CAATTGGCTT GAGACTGAAA ATCTGCCCGA ATTGTATGCC 
ATCAAACTTA ATCACCACTC CATCCACATG GAGTCGCTGG CCATAAGGGA ACGCATTCTG 
GGCCGCAATA ATCCGGAGTT GCCTCAGGCA ATTATCTATC GAGGAGCTGT GATGGCGGAT 
CAGGGCAGGT TTTACCAGTG CCAGGTGCTT TGGAACTACG CCATAGATCT GCGAATGCGC 
AATAATGTAT CGGTAGATCG CGATCTCTTA CGGTTCGCCC AGCTTTTTGC TCAGATTTTG 
CGGGTGGAGA ACCACAATCT TACGTTGGAT CACGTTCTGC CAGTTTTGGC CAAGTGTCAG 
CAGGAGATCG AGAACAATAA GTTTAAAATT AAGGAAGCCA AGCCCAACGC TTGCCCCAGT 
CTATGGCAGG ATCAGAATAA TCAGAACTGC ATTACTGCAC TCTATCTAAT CAAGATCGTT 
ACCCATTTGG CGCGACGCAA GAAGGATCAA AACATTGACG AGGAGCACAT CCAGCAGCTA 
TTCCTGGTCG TACGCAAGTT CATACAGAAC GACACTCGCC TGCAGGATGG ACAGACATTG 
CTGCATATCG CCGTCAATGG AGTGATGCCC GTAGACGAGT TCTACACAAA TGAGATGTGC 
AGATTTCCCT GCTATGCCAC TGCTTTGGTT CTGGTACACT GTGGCGCCTC CGTGGTTGCA 
GTGGACTCGG ATCGGAACAC CCCTCTACAC ATCCTAGTGA CCAAGGTGAA CACCACTCAG 
GATCGCCAGG CCGAAATGGC TCGCATTCTG GAGCTGTTCG TCGAGGCAGG AGCCCATTTG 
GATGCAGTGA ACGCGGCAGG GCAGACAGCG GCCACGGCCT GCAAGCTGCC TATCTTGGCC 
AATCGGCTGC ACGCCCATCA GAATGCCCAC ACCAGCCTCA AGTGCCTGGC CGCACGCTCA 
ATTGCCACCA ACAGGCTAAA CTTCAAGGGT CTGATACCCA CGCAACTGGA GGCCTTCATA 
CAGATGCACA GCGTGCGCAA GGTGCTGCCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725993.1
CDS	76..2028
Translation	MHAELYRTRKRWYESAWPGAGFWRGANDTLPVTNYITYPANFDQPDGQSLTPLTMAAMMGNVKFVKTLLSHYDVDLDRECNVIYDGMVVYGATALWVAAGMGHLQIVKMLVQAGAAINHNTKAQSSPLRAACYEGRLDIVEFLIDNGADVNATNLFNNNTLMIAAYKGHHLVVKTLLQNGSRANDQALCGATALHYAAESGHLDVVVALLDHGATLKKNELGITPALQAAERLHEDVLEAFIERPELMSLEEQITALELLGATYANDKIKYDVNKAYGYLLRAMQLRYSNPRGVIRKRVQPTVPAYDNWLETENLPELYAIKLNHHSIHMESLAIRERILGRNNPELPQAIIYRGAVMADQGRFYQCQVLWNYAIDLRMRNNVSVDRDLLRFAQLFAQILRVENHNLTLDHVLPVLAKCQQEIENNKFKIKEAKPNACPSLWQDQNNQNCITALYLIKIVTHLARRKKDQNIDEEHIQQLFLVVRKFIQNDTRLQDGQTLLHIAVNGVMPVDEFYTNEMCRFPCYATALVLVHCGASVVAVDSDRNTPLHILVTKVNTTQDRQAEMARILELFVEAGAHLDAVNAAGQTAATACKLPILANRLHAHQNAHTSLKCLAARSIATNRLNFKGLIPTQLEAFIQMHSVRKVLP

Target ORF information:

RefSeq Version	NM_166408.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Fem-1, transcript variant B (Fem-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166408.2

ORF Insert Sequence:

ATGCACGCGG AGCTGTATCG CACTCGCAAG CGGTGGTATG AGTCCGCTTG GCCTGGAGCA 
GGATTCTGGC GAGGAGCCAA CGACACGCTG CCCGTGACGA ACTACATCAC GTATCCGGCG 
AATTTCGATC AGCCGGATGG ACAATCGCTA ACACCCCTGA CCATGGCCGC GATGATGGGC 
AATGTGAAAT TCGTGAAGAC TCTGCTGTCG CACTACGACG TCGACTTGGA CCGAGAATGC 
AATGTCATCT ACGACGGGAT GGTGGTCTAC GGAGCCACAG CCCTGTGGGT GGCTGCCGGA 
ATGGGGCATC TGCAGATAGT CAAAATGCTC GTCCAAGCGG GAGCGGCCAT CAATCACAAT 
ACCAAGGCGC AGTCGTCGCC GCTGAGAGCG GCCTGCTATG AGGGTCGATT GGACATCGTT 
GAATTCCTAA TTGACAATGG CGCTGATGTG AATGCCACGA ATTTGTTCAA CAACAACACG 
CTTATGATTG CCGCCTACAA GGGTCACCAC CTGGTGGTAA AGACTCTACT TCAGAATGGA 
TCAAGGGCCA ATGATCAGGC GCTGTGCGGC GCCACTGCCT TGCACTATGC GGCCGAAAGC 
GGTCACCTGG ATGTGGTGGT AGCCCTGCTT GACCATGGCG CCACTCTGAA GAAGAATGAG 
CTGGGAATTA CGCCCGCCTT GCAGGCTGCC GAACGTCTCC ATGAGGATGT CCTTGAAGCG 
TTCATCGAAC GACCAGAACT GATGAGCTTG GAGGAGCAGA TCACAGCATT GGAGCTTCTG 
GGAGCCACTT ATGCGAATGA CAAAATCAAA TACGACGTGA ATAAGGCCTA TGGCTATCTG 
CTGAGAGCCA TGCAGCTACG TTACAGCAAT CCACGCGGTG TGATCCGCAA AAGAGTTCAG 
CCCACTGTGC CCGCGTATGA CAATTGGCTT GAGACTGAAA ATCTGCCCGA ATTGTATGCC 
ATCAAACTTA ATCACCACTC CATCCACATG GAGTCGCTGG CCATAAGGGA ACGCATTCTG 
GGCCGCAATA ATCCGGAGTT GCCTCAGGCA ATTATCTATC GAGGAGCTGT GATGGCGGAT 
CAGGGCAGGT TTTACCAGTG CCAGGTGCTT TGGAACTACG CCATAGATCT GCGAATGCGC 
AATAATGTAT CGGTAGATCG CGATCTCTTA CGGTTCGCCC AGCTTTTTGC TCAGATTTTG 
CGGGTGGAGA ACCACAATCT TACGTTGGAT CACGTTCTGC CAGTTTTGGC CAAGTGTCAG 
CAGGAGATCG AGAACAATAA GTTTAAAATT AAGGAAGCCA AGCCCAACGC TTGCCCCAGT 
CTATGGCAGG ATCAGAATAA TCAGAACTGC ATTACTGCAC TCTATCTAAT CAAGATCGTT 
ACCCATTTGG CGCGACGCAA GAAGGATCAA AACATTGACG AGGAGCACAT CCAGCAGCTA 
TTCCTGGTCG TACGCAAGTT CATACAGAAC GACACTCGCC TGCAGGATGG ACAGACATTG 
CTGCATATCG CCGTCAATGG AGTGATGCCC GTAGACGAGT TCTACACAAA TGAGATGTGC 
AGATTTCCCT GCTATGCCAC TGCTTTGGTT CTGGTACACT GTGGCGCCTC CGTGGTTGCA 
GTGGACTCGG ATCGGAACAC CCCTCTACAC ATCCTAGTGA CCAAGGTGAA CACCACTCAG 
GATCGCCAGG CCGAAATGGC TCGCATTCTG GAGCTGTTCG TCGAGGCAGG AGCCCATTTG 
GATGCAGTGA ACGCGGCAGG GCAGACAGCG GCCACGGCCT GCAAGCTGCC TATCTTGGCC 
AATCGGCTGC ACGCCCATCA GAATGCCCAC ACCAGCCTCA AGTGCCTGGC CGCACGCTCA 
ATTGCCACCA ACAGGCTAAA CTTCAAGGGT CTGATACCCA CGCAACTGGA GGCCTTCATA 
CAGATGCACA GCGTGCGCAA GGTGCTGCCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fem-1 ( NM_137664.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611508.1 Drosophila melanogaster Fem-1, transcript variant A (Fem-1), mRNA.

Price & Availability↑

CloneID	OFa10700
Clone ID Related Accession (Same CDS sequence)	NM_137664.3
Accession Version	NM_137664.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Fem-1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137664.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGTCG ATCCCGCAGA AGAGGCGCCT CTAGACGAGG CACATACGTC CATATACGAC 
GGCTATCCGC CCGATTTCGG CTACTTCAAC ATGGCCCACG GAATGCTGGT GGAGATCTCC 
GAGGAGATCC GGGCCAAGTT TCCCCACGAG CCGCGTCTAC GTTTCTCCCA GCTAAACAAC 
CTTCACCTAG CGGCCCGCAG TGGTCAGATG TTGAGCTTCG TCGAGATCTT GAATTCGGTT 
AGCTGCAGGG CGGTGCAGAC CCACCTGGTG AATACAAATT TCGATCAGCC GGATGGACAA 
TCGCTAACAC CCCTGACCAT GGCCGCGATG ATGGGCAATG TGAAATTCGT GAAGACTCTG 
CTGTCGCACT ACGACGTCGA CTTGGACCGA GAATGCAATG TCATCTACGA CGGGATGGTG 
GTCTACGGAG CCACAGCCCT GTGGGTGGCT GCCGGAATGG GGCATCTGCA GATAGTCAAA 
ATGCTCGTCC AAGCGGGAGC GGCCATCAAT CACAATACCA AGGCGCAGTC GTCGCCGCTG 
AGAGCGGCCT GCTATGAGGG TCGATTGGAC ATCGTTGAAT TCCTAATTGA CAATGGCGCT 
GATGTGAATG CCACGAATTT GTTCAACAAC AACACGCTTA TGATTGCCGC CTACAAGGGT 
CACCACCTGG TGGTAAAGAC TCTACTTCAG AATGGATCAA GGGCCAATGA TCAGGCGCTG 
TGCGGCGCCA CTGCCTTGCA CTATGCGGCC GAAAGCGGTC ACCTGGATGT GGTGGTAGCC 
CTGCTTGACC ATGGCGCCAC TCTGAAGAAG AATGAGCTGG GAATTACGCC CGCCTTGCAG 
GCTGCCGAAC GTCTCCATGA GGATGTCCTT GAAGCGTTCA TCGAACGACC AGAACTGATG 
AGCTTGGAGG AGCAGATCAC AGCATTGGAG CTTCTGGGAG CCACTTATGC GAATGACAAA 
ATCAAATACG ACGTGAATAA GGCCTATGGC TATCTGCTGA GAGCCATGCA GCTACGTTAC 
AGCAATCCAC GCGGTGTGAT CCGCAAAAGA GTTCAGCCCA CTGTGCCCGC GTATGACAAT 
TGGCTTGAGA CTGAAAATCT GCCCGAATTG TATGCCATCA AACTTAATCA CCACTCCATC 
CACATGGAGT CGCTGGCCAT AAGGGAACGC ATTCTGGGCC GCAATAATCC GGAGTTGCCT 
CAGGCAATTA TCTATCGAGG AGCTGTGATG GCGGATCAGG GCAGGTTTTA CCAGTGCCAG 
GTGCTTTGGA ACTACGCCAT AGATCTGCGA ATGCGCAATA ATGTATCGGT AGATCGCGAT 
CTCTTACGGT TCGCCCAGCT TTTTGCTCAG ATTTTGCGGG TGGAGAACCA CAATCTTACG 
TTGGATCACG TTCTGCCAGT TTTGGCCAAG TGTCAGCAGG AGATCGAGAA CAATAAGTTT 
AAAATTAAGG AAGCCAAGCC CAACGCTTGC CCCAGTCTAT GGCAGGATCA GAATAATCAG 
AACTGCATTA CTGCACTCTA TCTAATCAAG ATCGTTACCC ATTTGGCGCG ACGCAAGAAG 
GATCAAAACA TTGACGAGGA GCACATCCAG CAGCTATTCC TGGTCGTACG CAAGTTCATA 
CAGAACGACA CTCGCCTGCA GGATGGACAG ACATTGCTGC ATATCGCCGT CAATGGAGTG 
ATGCCCGTAG ACGAGTTCTA CACAAATGAG ATGTGCAGAT TTCCCTGCTA TGCCACTGCT 
TTGGTTCTGG TACACTGTGG CGCCTCCGTG GTTGCAGTGG ACTCGGATCG GAACACCCCT 
CTACACATCC TAGTGACCAA GGTGAACACC ACTCAGGATC GCCAGGCCGA AATGGCTCGC 
ATTCTGGAGC TGTTCGTCGA GGCAGGAGCC CATTTGGATG CAGTGAACGC GGCAGGGCAG 
ACAGCGGCCA CGGCCTGCAA GCTGCCTATC TTGGCCAATC GGCTGCACGC CCATCAGAAT 
GCCCACACCA GCCTCAAGTG CCTGGCCGCA CGCTCAATTG CCACCAACAG GCTAAACTTC 
AAGGGTCTGA TACCCACGCA ACTGGAGGCC TTCATACAGA TGCACAGCGT GCGCAAGGTG 
CTGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611508.1
CDS	113..2221
Translation	MDVDPAEEAPLDEAHTSIYDGYPPDFGYFNMAHGMLVEISEEIRAKFPHEPRLRFSQLNNLHLAARSGQMLSFVEILNSVSCRAVQTHLVNTNFDQPDGQSLTPLTMAAMMGNVKFVKTLLSHYDVDLDRECNVIYDGMVVYGATALWVAAGMGHLQIVKMLVQAGAAINHNTKAQSSPLRAACYEGRLDIVEFLIDNGADVNATNLFNNNTLMIAAYKGHHLVVKTLLQNGSRANDQALCGATALHYAAESGHLDVVVALLDHGATLKKNELGITPALQAAERLHEDVLEAFIERPELMSLEEQITALELLGATYANDKIKYDVNKAYGYLLRAMQLRYSNPRGVIRKRVQPTVPAYDNWLETENLPELYAIKLNHHSIHMESLAIRERILGRNNPELPQAIIYRGAVMADQGRFYQCQVLWNYAIDLRMRNNVSVDRDLLRFAQLFAQILRVENHNLTLDHVLPVLAKCQQEIENNKFKIKEAKPNACPSLWQDQNNQNCITALYLIKIVTHLARRKKDQNIDEEHIQQLFLVVRKFIQNDTRLQDGQTLLHIAVNGVMPVDEFYTNEMCRFPCYATALVLVHCGASVVAVDSDRNTPLHILVTKVNTTQDRQAEMARILELFVEAGAHLDAVNAAGQTAATACKLPILANRLHAHQNAHTSLKCLAARSIATNRLNFKGLIPTQLEAFIQMHSVRKVLP

Target ORF information:

RefSeq Version	NM_137664.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Fem-1, transcript variant A (Fem-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137664.3

ORF Insert Sequence:

ATGGACGTCG ATCCCGCAGA AGAGGCGCCT CTAGACGAGG CACATACGTC CATATACGAC 
GGCTATCCGC CCGATTTCGG CTACTTCAAC ATGGCCCACG GAATGCTGGT GGAGATCTCC 
GAGGAGATCC GGGCCAAGTT TCCCCACGAG CCGCGTCTAC GTTTCTCCCA GCTAAACAAC 
CTTCACCTAG CGGCCCGCAG TGGTCAGATG TTGAGCTTCG TCGAGATCTT GAATTCGGTT 
AGCTGCAGGG CGGTGCAGAC CCACCTGGTG AATACAAATT TCGATCAGCC GGATGGACAA 
TCGCTAACAC CCCTGACCAT GGCCGCGATG ATGGGCAATG TGAAATTCGT GAAGACTCTG 
CTGTCGCACT ACGACGTCGA CTTGGACCGA GAATGCAATG TCATCTACGA CGGGATGGTG 
GTCTACGGAG CCACAGCCCT GTGGGTGGCT GCCGGAATGG GGCATCTGCA GATAGTCAAA 
ATGCTCGTCC AAGCGGGAGC GGCCATCAAT CACAATACCA AGGCGCAGTC GTCGCCGCTG 
AGAGCGGCCT GCTATGAGGG TCGATTGGAC ATCGTTGAAT TCCTAATTGA CAATGGCGCT 
GATGTGAATG CCACGAATTT GTTCAACAAC AACACGCTTA TGATTGCCGC CTACAAGGGT 
CACCACCTGG TGGTAAAGAC TCTACTTCAG AATGGATCAA GGGCCAATGA TCAGGCGCTG 
TGCGGCGCCA CTGCCTTGCA CTATGCGGCC GAAAGCGGTC ACCTGGATGT GGTGGTAGCC 
CTGCTTGACC ATGGCGCCAC TCTGAAGAAG AATGAGCTGG GAATTACGCC CGCCTTGCAG 
GCTGCCGAAC GTCTCCATGA GGATGTCCTT GAAGCGTTCA TCGAACGACC AGAACTGATG 
AGCTTGGAGG AGCAGATCAC AGCATTGGAG CTTCTGGGAG CCACTTATGC GAATGACAAA 
ATCAAATACG ACGTGAATAA GGCCTATGGC TATCTGCTGA GAGCCATGCA GCTACGTTAC 
AGCAATCCAC GCGGTGTGAT CCGCAAAAGA GTTCAGCCCA CTGTGCCCGC GTATGACAAT 
TGGCTTGAGA CTGAAAATCT GCCCGAATTG TATGCCATCA AACTTAATCA CCACTCCATC 
CACATGGAGT CGCTGGCCAT AAGGGAACGC ATTCTGGGCC GCAATAATCC GGAGTTGCCT 
CAGGCAATTA TCTATCGAGG AGCTGTGATG GCGGATCAGG GCAGGTTTTA CCAGTGCCAG 
GTGCTTTGGA ACTACGCCAT AGATCTGCGA ATGCGCAATA ATGTATCGGT AGATCGCGAT 
CTCTTACGGT TCGCCCAGCT TTTTGCTCAG ATTTTGCGGG TGGAGAACCA CAATCTTACG 
TTGGATCACG TTCTGCCAGT TTTGGCCAAG TGTCAGCAGG AGATCGAGAA CAATAAGTTT 
AAAATTAAGG AAGCCAAGCC CAACGCTTGC CCCAGTCTAT GGCAGGATCA GAATAATCAG 
AACTGCATTA CTGCACTCTA TCTAATCAAG ATCGTTACCC ATTTGGCGCG ACGCAAGAAG 
GATCAAAACA TTGACGAGGA GCACATCCAG CAGCTATTCC TGGTCGTACG CAAGTTCATA 
CAGAACGACA CTCGCCTGCA GGATGGACAG ACATTGCTGC ATATCGCCGT CAATGGAGTG 
ATGCCCGTAG ACGAGTTCTA CACAAATGAG ATGTGCAGAT TTCCCTGCTA TGCCACTGCT 
TTGGTTCTGG TACACTGTGG CGCCTCCGTG GTTGCAGTGG ACTCGGATCG GAACACCCCT 
CTACACATCC TAGTGACCAA GGTGAACACC ACTCAGGATC GCCAGGCCGA AATGGCTCGC 
ATTCTGGAGC TGTTCGTCGA GGCAGGAGCC CATTTGGATG CAGTGAACGC GGCAGGGCAG 
ACAGCGGCCA CGGCCTGCAA GCTGCCTATC TTGGCCAATC GGCTGCACGC CCATCAGAAT 
GCCCACACCA GCCTCAAGTG CCTGGCCGCA CGCTCAATTG CCACCAACAG GCTAAACTTC 
AAGGGTCTGA TACCCACGCA ACTGGAGGCC TTCATACAGA TGCACAGCGT GCGCAAGGTG 
CTGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Fem-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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