Fem1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fem1b
Entrez Gene ID	315745
Full Name	fem-1 homolog B
General protein information	Preferred Names fem-1 homolog B Names protein fem-1 homolog B FEM1-beta feminization 1 homolog b
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001108157.1	NP_001101627.1	protein fem-1 homolog B

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	FEM1B	NP_001025724.1
Danio rerio (zebrafish)	fem1b	XP_695502.4
Xenopus tropicalis (tropical clawed frog)	fem1b	NP_001238770.1
Homo sapiens (human)	FEM1B	NP_056137.1
Pan troglodytes (chimpanzee)	FEM1B	XP_523110.2
Bos taurus (cattle)	FEM1B	NP_001179312.1
Mus musculus (house mouse)	Fem1b	NP_034323.1
Rattus norvegicus (Norway rat)	Fem1b	NP_001101627.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007382	XP_308449.4
	FEM1B	XP_001083413.1
Canis lupus familiaris (dog)	FEM1B	XP_535526.3
Drosophila melanogaster (fruit fly)	Fem-1	NP_611508.1

Gene Ontology
Bibliography

Related articles in PubMed

Mouse Fem1b interacts with and induces ubiquitin-mediated degradation of Ankrd37.
Shi YQ, Liao SY, Zhuang XJ, Han CS
Gene485(2)153-9(2011 Oct)

Uncovering early response of gene regulatory networks in ESCs by systematic induction of transcription factors.
Nishiyama A, Xin L, Sharov AA, Thomas M, Mowrer G, Meyers E, Piao Y, Mehta S, Yee S, Nakatake Y, Stagg C, Sharova L, Correa-Cerro LS, Bassey U, Hoang H, Kim E, Tapnio R, Qian Y, Dudekula D, Zalzman M, Li M, Falco G, Yang HT, Lee SL, Monti M, Stanghellini I, Islam MN, Nagaraja R, Goldberg I, Wang W, Longo DL, Schlessinger D, Ko MS
Cell stem cell5(4)420-33(2009 Oct)

Human FEM1B is required for Rad9 recruitment and CHK1 activation in response to replication stress.
Sun TP, Shieh SY
Oncogene28(18)1971-81(2009 May)

Mouse Fem1b interacts with the Nkx3.1 homeoprotein and is required for proper male secondary sexual development.
Wang X, Desai N, Hu YP, Price SM, Abate-Shen C, Shen MM
Developmental dynamics : an official publication of the American Association of Anatomists237(10)2963-72(2008 Oct)

VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases.
Kamura T, Maenaka K, Kotoshiba S, Matsumoto M, Kohda D, Conaway RC, Conaway JW, Nakayama KI
Genes & development18(24)3055-65(2004 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Fem1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fem1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06782	NM_001108157.1 Latest version!	Rattus norvegicus fem-1 homolog B (Fem1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fem1b ( NM_001108157.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101627.1 Rattus norvegicus fem-1 homolog B (Fem1b), mRNA.

Price & Availability↑

CloneID	ORa06782
Clone ID Related Accession (Same CDS sequence)	NM_001108157.1
Accession Version	NM_001108157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	protein fem-1 homolog B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473975.1. On or before Oct 4, 2007 this sequence version replaced XM_236321.3, XM_001074110.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGGCC TGGCTGGTTA TGTGTACAAG GCGGCCAGCG AGGGCAAGGT GCTCACTCTG 
GCTGCCTTGC TCCTTAACCG GTCAGAAAGC GATATCCGCT ACCTGCTGGG CTATGTCAGT 
CAGCAGGGAG GACAGCGCTC CACACCCCTC ATCATCGCAG CCCGCAATGG GCACGCCAAG 
GTGGTGCGCT TGCTGTTAGA ACACTACCGT GTGCAGACCC AGCAGACTGG CACCGTCCGC 
TTCGACGGGT ATGTCATTGA TGGTGCCACT GCTCTTTGGT GTGCTGCGGG AGCCGGACAT 
TTTGAAGTTG TTAAACTTCT AGTCAGCCAT GGAGCCAACG TGAACCACAC CACAGTCACT 
AACTCAACTC CATTGCGGGC AGCGTGCTTT GATGGTAGAC TGGACATTGT GAAATATTTG 
GTTGAAAATA ATGCCAATAT CAGCATTGCC AACAAGTATG ACAACACCTG CCTAATGATC 
GCAGCATATA AGGGGCACAC CGATGTTGTC AGATACCTCT TAGAACAGCG TGCTGACCCC 
AATGCTAAAG CACACTGTGG AGCCACAGCT TTGCACTTCG CAGCCGAAGC TGGTCACATT 
GACATTGTGA AAGAACTGAT AAAATGGCGA GCCGCAATAG TAGTAAATGG CCACGGGATG 
ACACCGTTGA AGGTAGCTGC TGAAAGCTGT AAAGCTGATG TCGTTGAACT GTTGCTCTCT 
CATGCCGATT GTGACCGCAG AAGTCGGATT GAAGCCTTGG AGCTCTTGGG TGCCTCCTTT 
GCAAATGACC GTGAGAACTA TGACATCATG AAGACATACC ACTATTTATA TTTAGCTATG 
TTGGAGAGGT TTCAGGATGG TGACAACATT CTTGAGAAAG AGGTTCTTCC ACCCATCCAT 
GCTTATGGGA ACAGAACTGA ATGTAGGAAC CCACAGGAAT TGGAGGCTAT TCGGCAAGAC 
AGAGATGCTC TTCACATGGA GGGCCTTATC GTGCGAGAAC GGATTTTAGG TGCTGACAAC 
ATTGATGTTT CCCATCCCAT CATTTACAGA GGGGCTGTCT ATGCTGACAA CATGGAGTTC 
GAGCAGTGCA TCAAATTGTG GCTTCACGCG CTGCACCTCA GACAGAAAGG AAATAGAAAT 
ACCCACAAGG ATCTGCTTCG GTTTGCTCAA GTCTTCTCAC AGATGATACA CCTCAATGAA 
GCTGTGAAGG CCCCAGACAT AGAATGCGTT TTGAGATGCA GTGTCTTGGA AATAGAGCAG 
AGCATGAACA GAGTTAAAAA TATCTCCGAT GCTGACGTCC ACAGTGCCAT GGATAACTAT 
GAGTGTAACC TCTATACCTT TCTGTACCTT GTGTGCATCT CCACCAAGAC ACAGTGTAGC 
GAAGAAGACC AGTGCAGAAT TAACAAGCAG ATCTACAACC TGATTCACCT GGACCCCAGA 
ACACGGGAAG GTTTCACCTT GCTACACTTG GCCGTCAACT CGAACACACC AGTCGATGAT 
TTCCACACCA ATGATGTCTG CAGCTTTCCC AATGCACTGG TCACAAAGCT CCTGCTGGAC 
TGTGGTGCTG AGGTAAATGC TGTGGACAAT GAGGGGAACA GTGCCCTCCA CATTATCGTC 
CAGTACAACA GGCCCATCAG TGATTTTCTG ACCTTGCACT CCATCATCAT TAGCCTTGTG 
GAAGCTGGAG CTCATACCGA CATGACAAAC AAGCAGAATA AAACTCCGCT AGACAAAAGT 
ACAACTGGGG TGTCTGAAAT ACTACTTAAA ACTCAGATGA AGATGAGCCT CAAGTGCCTG 
GCTGCCCGAG CAGTTCGGGC TAATGACATT AACTACCAAG ACCAGATCCC CCGGACTCTT 
GAAGAGTTTG TTGGATTTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101627.1
CDS	254..2137
Translation	MEGLAGYVYKAASEGKVLTLAALLLNRSESDIRYLLGYVSQQGGQRSTPLIIAARNGHAKVVRLLLEHYRVQTQQTGTVRFDGYVIDGATALWCAAGAGHFEVVKLLVSHGANVNHTTVTNSTPLRAACFDGRLDIVKYLVENNANISIANKYDNTCLMIAAYKGHTDVVRYLLEQRADPNAKAHCGATALHFAAEAGHIDIVKELIKWRAAIVVNGHGMTPLKVAAESCKADVVELLLSHADCDRRSRIEALELLGASFANDRENYDIMKTYHYLYLAMLERFQDGDNILEKEVLPPIHAYGNRTECRNPQELEAIRQDRDALHMEGLIVRERILGADNIDVSHPIIYRGAVYADNMEFEQCIKLWLHALHLRQKGNRNTHKDLLRFAQVFSQMIHLNEAVKAPDIECVLRCSVLEIEQSMNRVKNISDADVHSAMDNYECNLYTFLYLVCISTKTQCSEEDQCRINKQIYNLIHLDPRTREGFTLLHLAVNSNTPVDDFHTNDVCSFPNALVTKLLLDCGAEVNAVDNEGNSALHIIVQYNRPISDFLTLHSIIISLVEAGAHTDMTNKQNKTPLDKSTTGVSEILLKTQMKMSLKCLAARAVRANDINYQDQIPRTLEEFVGFH

Target ORF information:

RefSeq Version	NM_001108157.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus fem-1 homolog B (Fem1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108157.1

ORF Insert Sequence:

ATGGAGGGCC TGGCTGGTTA TGTGTACAAG GCGGCCAGCG AGGGCAAGGT GCTCACTCTG 
GCTGCCTTGC TCCTTAACCG GTCAGAAAGC GATATCCGCT ACCTGCTGGG CTATGTCAGT 
CAGCAGGGAG GACAGCGCTC CACACCCCTC ATCATCGCAG CCCGCAATGG GCACGCCAAG 
GTGGTGCGCT TGCTGTTAGA ACACTACCGT GTGCAGACCC AGCAGACTGG CACCGTCCGC 
TTCGACGGGT ATGTCATTGA TGGTGCCACT GCTCTTTGGT GTGCTGCGGG AGCCGGACAT 
TTTGAAGTTG TTAAACTTCT AGTCAGCCAT GGAGCCAACG TGAACCACAC CACAGTCACT 
AACTCAACTC CATTGCGGGC AGCGTGCTTT GATGGTAGAC TGGACATTGT GAAATATTTG 
GTTGAAAATA ATGCCAATAT CAGCATTGCC AACAAGTATG ACAACACCTG CCTAATGATC 
GCAGCATATA AGGGGCACAC CGATGTTGTC AGATACCTCT TAGAACAGCG TGCTGACCCC 
AATGCTAAAG CACACTGTGG AGCCACAGCT TTGCACTTCG CAGCCGAAGC TGGTCACATT 
GACATTGTGA AAGAACTGAT AAAATGGCGA GCCGCAATAG TAGTAAATGG CCACGGGATG 
ACACCGTTGA AGGTAGCTGC TGAAAGCTGT AAAGCTGATG TCGTTGAACT GTTGCTCTCT 
CATGCCGATT GTGACCGCAG AAGTCGGATT GAAGCCTTGG AGCTCTTGGG TGCCTCCTTT 
GCAAATGACC GTGAGAACTA TGACATCATG AAGACATACC ACTATTTATA TTTAGCTATG 
TTGGAGAGGT TTCAGGATGG TGACAACATT CTTGAGAAAG AGGTTCTTCC ACCCATCCAT 
GCTTATGGGA ACAGAACTGA ATGTAGGAAC CCACAGGAAT TGGAGGCTAT TCGGCAAGAC 
AGAGATGCTC TTCACATGGA GGGCCTTATC GTGCGAGAAC GGATTTTAGG TGCTGACAAC 
ATTGATGTTT CCCATCCCAT CATTTACAGA GGGGCTGTCT ATGCTGACAA CATGGAGTTC 
GAGCAGTGCA TCAAATTGTG GCTTCACGCG CTGCACCTCA GACAGAAAGG AAATAGAAAT 
ACCCACAAGG ATCTGCTTCG GTTTGCTCAA GTCTTCTCAC AGATGATACA CCTCAATGAA 
GCTGTGAAGG CCCCAGACAT AGAATGCGTT TTGAGATGCA GTGTCTTGGA AATAGAGCAG 
AGCATGAACA GAGTTAAAAA TATCTCCGAT GCTGACGTCC ACAGTGCCAT GGATAACTAT 
GAGTGTAACC TCTATACCTT TCTGTACCTT GTGTGCATCT CCACCAAGAC ACAGTGTAGC 
GAAGAAGACC AGTGCAGAAT TAACAAGCAG ATCTACAACC TGATTCACCT GGACCCCAGA 
ACACGGGAAG GTTTCACCTT GCTACACTTG GCCGTCAACT CGAACACACC AGTCGATGAT 
TTCCACACCA ATGATGTCTG CAGCTTTCCC AATGCACTGG TCACAAAGCT CCTGCTGGAC 
TGTGGTGCTG AGGTAAATGC TGTGGACAAT GAGGGGAACA GTGCCCTCCA CATTATCGTC 
CAGTACAACA GGCCCATCAG TGATTTTCTG ACCTTGCACT CCATCATCAT TAGCCTTGTG 
GAAGCTGGAG CTCATACCGA CATGACAAAC AAGCAGAATA AAACTCCGCT AGACAAAAGT 
ACAACTGGGG TGTCTGAAAT ACTACTTAAA ACTCAGATGA AGATGAGCCT CAAGTGCCTG 
GCTGCCCGAG CAGTTCGGGC TAATGACATT AACTACCAAG ACCAGATCCC CCGGACTCTT 
GAAGAGTTTG TTGGATTTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mouse Fem1b interacts with and induces ubiquitin-mediated degradation of Ankrd37.
Gene485(2)153-9(2011 Oct)
Shi YQ,Liao SY,Zhuang XJ,Han CS

Uncovering early response of gene regulatory networks in ESCs by systematic induction of transcription factors.
Cell stem cell5(4)420-33(2009 Oct)
Nishiyama A,Xin L,Sharov AA,Thomas M,Mowrer G,Meyers E,Piao Y,Mehta S,Yee S,Nakatake Y,Stagg C,Sharova L,Correa-Cerro LS,Bassey U,Hoang H,Kim E,Tapnio R,Qian Y,Dudekula D,Zalzman M,Li M,Falco G,Yang HT,Lee SL,Monti M,Stanghellini I,Islam MN,Nagaraja R,Goldberg I,Wang W,Longo DL,Schlessinger D,Ko MS

Human FEM1B is required for Rad9 recruitment and CHK1 activation in response to replication stress.
Oncogene28(18)1971-81(2009 May)
Sun TP,Shieh SY

Mouse Fem1b interacts with the Nkx3.1 homeoprotein and is required for proper male secondary sexual development.
Developmental dynamics : an official publication of the American Association of Anatomists237(10)2963-72(2008 Oct)
Wang X,Desai N,Hu YP,Price SM,Abate-Shen C,Shen MM

VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases.
Genes & development18(24)3055-65(2004 Dec)
Kamura T,Maenaka K,Kotoshiba S,Matsumoto M,Kohda D,Conaway RC,Conaway JW,Nakayama KI

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Fem1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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