TRIM71 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRIM71
Entrez Gene ID	428445
Full Name	tripartite motif containing 71
Synonyms	LIN-41,LIN41
General protein information	Preferred Names tripartite motif containing 71 Names E3 ubiquitin-protein ligase TRIM71 RING-type E3 ubiquitin transferase TRIM71 abnormal cell LINeage LIN-41 lin-41 homolog protein lin-41 homolog tripartite motif containing 71, E3 ubiquitin protein ligase tripartite motif-containing 71 tripartite motif-containing protein 71
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025147327.1	XP_025003095.1	E3 ubiquitin-protein ligase TRIM71 isoform X2
NM_001037275.2	NP_001032352.2	E3 ubiquitin-protein ligase TRIM71
XM_025147326.1	XP_025003094.1	E3 ubiquitin-protein ligase TRIM71 isoform X1
XM_025147328.1	XP_025003096.1	E3 ubiquitin-protein ligase TRIM71 isoform X2

Pathways from BioSystems

Homologies

	TRIM71	XP_001098706.1
Bos taurus (cattle)	TRIM71	XP_002707823.1
Gallus gallus (chicken)	TRIM71	NP_001032352.2
Pan troglodytes (chimpanzee)	TRIM71	XP_516352.3
Rattus norvegicus (Norway rat)	Trim71	NP_001178730.1
Danio rerio (zebrafish)	trim71	XP_690252.5
Caenorhabditis elegans (roundworm)	lin-41	NP_001020998.1
Mus musculus (house mouse)	Trim71	NP_001035968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005125	XP_314006.4
Homo sapiens (human)	TRIM71	NP_001034200.1
Canis lupus familiaris (dog)	TRIM71	XP_534214.3
Drosophila melanogaster (fruit fly)	wech	NP_001137614.1
Xenopus tropicalis (tropical clawed frog)	LOC100486898	XP_004915429.1

Gene Ontology
Bibliography

Related articles in PubMed

Cloning and regulation of the vertebrate homologue of lin-41 that functions as a heterochronic gene in Caenorhabditis elegans.
Kanamoto T, Terada K, Yoshikawa H, Furukawa T
Developmental dynamics : an official publication of the American Association of Anatomists235(4)1142-9(2006 Apr)

Reciprocal expression of lin-41 and the microRNAs let-7 and mir-125 during mouse embryogenesis.
Schulman BR, Esquela-Kerscher A, Slack FJ
Developmental dynamics : an official publication of the American Association of Anatomists234(4)1046-54(2005 Dec)

Analysis of the regulation of lin-41 during chick and mouse limb development.
Lancman JJ, Caruccio NC, Harfe BD, Pasquinelli AE, Schageman JJ, Pertsemlidis A, Fallon JF
Developmental dynamics : an official publication of the American Association of Anatomists234(4)948-60(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lin-41 is genetically and biochemically downstream of both the Shh and Fgf signaling pathways and is expressed in three phases over developmental time and most notably is associated with the developing autopod.
Title: Analysis of the regulation of lin-41 during chick and mouse limb development.

The following TRIM71 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRIM71 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa145951	XM_025147326.1 Latest version!	Gallus gallus tripartite motif containing 71 (TRIM71), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa145952	XM_025147327.1 Latest version!	Gallus gallus tripartite motif containing 71 (TRIM71), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa145952	XM_025147328.1 Latest version!	Gallus gallus tripartite motif containing 71 (TRIM71), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa27758	NM_001037275.2 Latest version!	Gallus gallus tripartite motif containing 71 (TRIM71), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRIM71 ( XM_025147326.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025003094.1 Gallus gallus tripartite motif containing 71 (TRIM71), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa145951
Clone ID Related Accession (Same CDS sequence)	XM_025147326.1
Accession Version	XM_025147326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIM71 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGT TCCCGGAGGC CGACTTCCAG GTGTGCCCGC TGTGCAAGGA GATGTGCGGC 
TCGCCCGCCC CGCTCTCCTC CAACTCGTCC ACCTCGTCGT CCTCGTCGCA GACCTCGGGC 
TCCTCGGGGG GCGGCGGCTC GTCGTGCGGG GGCGCGGCGC GGCGGCTGCA CGTCCTGCCC 
TGCCTGCACG CCTTCTGCCG CCAGTGCCTG GAGGCGCAGC GGCACCCCGC CGCGGGGGAC 
GCGCTCAAGC TGCGCTGCCC CATCTGCGAC CAGAAGGTGG TGATCTCGGA GCCCTCGGGC 
ATGGACGCGC TGCCCTCCTC CAACTTCCTC CTCAGCAACC TGCTGGACGT CGTGGTGGTG 
GCGGCCGCCG CCGACGAGCA GAAGAACGGC CGCGCCGCCG GCTCCGGGCC CGCCGCCGGC 
TCCGGCGCGG GGGGCGGCGG GGCCAACAAC CGCCACCACG GCCGCCCGCC GCCGCACCGC 
GCCGCCGCCG CCGCCGCCTC CTCGCCCGCC GCCGGCTCCG CCGCGCCCTC CGCCTCCTCG 
TCCTCCTCCT CGGGCGGCGG CGGCCCGGCG GCGCTGCTGC TGCGGCGGCC GCACAGCCGG 
CAGGGCGAGC CGCGCTGCAG CTCCTGCGAC GAGGGCAACG CCGCCAGCTC GCGCTGCCTG 
GACTGCCAGG AGCACCTGTG CGACAACTGC GTGCGGGCGC ACCAGCGCGT CCGCCTCACC 
AAGGACCACT TCATCGAGCG CTTCGCCGCC GGCCCCGCCG CCGCCTCCGC CGGCCCCGCC 
GCGCCGCTCG CCCTCAGCCC GCCCTACCCC GCCTCGCCCT ACAACATCCT CTCCGTCTTC 
CCCGAGCGGG CCAGCTACTG CCAGCACCAC GACGACGAGG TACTGCATTT CTACTGTGAT 
ACCTGCTCTG TCCCCATATG TCGTGAATGT ACCATGGGGC GACATGTGGG TCACAGCTTT 
ATCTACCTCC AAGATGCCCT CCAGGACTCC AGAACTCTTA CCATCCAGCT CCTGGCAGAT 
GCTCAGCAAG GACGACAGGC TATTCAGTTG AGTATTGAAC AGGCCCAGGC TGTGGCAGAG 
CAAGTTGAGA TGAAAGCGAA GGTGGTACAG TCTGAAGTCA AAGCAGTGAC TACCAGACAT 
AAGAAGGCCT TGGAAGAGCG AGAGTGCGAG CTGCTCTGGA AGGTGGAAAA GATTCGTCAA 
GTCAAGGCTA AGTCGCTCTA CTTGCAAGTT GAAAAGTTAC GTCAGAACCT AAATAAGCTG 
GACAACACTA TTAGTGCTGT TCAGCAGGTC CTGGAGGAAG GCCGTACCAT AGATATTCTT 
CTGGCCCGGG ACCGCATGCT GGCTCAGGTG CAGGAGCTCA AGAATGTGAG AGGCCTTCTG 
CAGCCACAGG AGGATGACAG GATCATGTTC ACGCCGCCTG ACCAGGCGTT GTATATGGCC 
ATTAAATCAA TGGGCTTTGT CAGTAGTGGG GCATTTGCTC CTCTGACCAA AGCCACTGGG 
GAAGGCCTCA AGCGTGCGCT GCAGGGCAAA GTAGCCTCTT TCACAGTTAT AGGCTACGAC 
CACGATGGTG AGCCTCGCCT TTCAGGAGGT GACATGATCT CTGCAGTGGT AATGGGCCCA 
GATGGAAATC TGTTTGGGGC AGACGTCAGT GATCAGCAGA ACGGAACCTA CCTGGTCAGC 
TACCGTCCGC AGCTAGAGGG AGAGCATCTG GTGTCCGTGA TGATGTGCAA CCAACACATT 
GAGAACAGCC CCTTCAAAGT TGTGGTGAAA TCTGGGCGCA GCTACATAGG TATTGGGCTA 
CCCGGGCTCT CGTTTGGCAG TGAAGGAGAC AGCGATGGGA AACTCTGCCG CCCCTGGGGG 
GTTAGCGTAG ACAAAGAAGG CTACATCATC GTGGCTGACC GCAGCAATAA CCGTATCCAG 
GTGTTCAAAC CATGTGGGAC TTTCCATCAC AAGTTTGGCA CGCTCGGTTC CCGGCCTGGC 
CAGTTCGATC GTCCCGCCGG TGTGGCCTGT GACGTCTCAC GCCGGATCGT CGTGGCTGAC 
AAGGACAATC ATCGCATCCA GATTTTCACG TTTGAGGGGC AGTTCATTCT GAAGTTTGGG 
GAGAAAGGAA CAAAGAATGG GCAATTCAAT TACCCGTGGG ACGTGGCCGT CAACGCAGAG 
GGCAAAATCC TGGTCTCCGA CACAAGGAAC CATCGCGTTC AGTTGTTCGG GCCCGATGGT 
GTGTTTCTTA ATAAGTACGG CTTTGAAGGG GCACTCTGGA AGCACTTTGA TTCCCCCAGA 
GGTGTGACTT TCAATCACGA GGGCCACTTG GTAGTGACAG ACTTCAACAA CCATCGCCTT 
CTGGTCATCC ATGCAGATTG CCAGTCAGCA CGCTTCCTGG GCTCGGAGGG GAGCGGGAAC 
GGGCAGTTCC TGCGCCCGCA GGGAGTGGCG GTGGATCAAG AGGGGCGCAT CATCGTGGCC 
GACTCCAGGA ACCACCGGGT GCAGATATTT GAGTCCAATG GTAGCTTTTT ATGCAAGTTC 
GGCGCTCAGG GAAGCGGCTT TGGTCAGATG GACCGTCCTT CAGGTATAGC TGTCACCCCT 
GATGGCATGA TTGTGGTGGT CGACTTTGGA AACAATCGAA TCCTCGTCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025003094.1
CDS	656..3289
Translation	MASFPEADFQVCPLCKEMCGSPAPLSSNSSTSSSSSQTSGSSGGGGSSCGGAARRLHVLPCLHAFCRQCLEAQRHPAAGDALKLRCPICDQKVVISEPSGMDALPSSNFLLSNLLDVVVVAAAADEQKNGRAAGSGPAAGSGAGGGGANNRHHGRPPPHRAAAAAASSPAAGSAAPSASSSSSSGGGGPAALLLRRPHSRQGEPRCSSCDEGNAASSRCLDCQEHLCDNCVRAHQRVRLTKDHFIERFAAGPAAASAGPAAPLALSPPYPASPYNILSVFPERASYCQHHDDEVLHFYCDTCSVPICRECTMGRHVGHSFIYLQDALQDSRTLTIQLLADAQQGRQAIQLSIEQAQAVAEQVEMKAKVVQSEVKAVTTRHKKALEERECELLWKVEKIRQVKAKSLYLQVEKLRQNLNKLDNTISAVQQVLEEGRTIDILLARDRMLAQVQELKNVRGLLQPQEDDRIMFTPPDQALYMAIKSMGFVSSGAFAPLTKATGEGLKRALQGKVASFTVIGYDHDGEPRLSGGDMISAVVMGPDGNLFGADVSDQQNGTYLVSYRPQLEGEHLVSVMMCNQHIENSPFKVVVKSGRSYIGIGLPGLSFGSEGDSDGKLCRPWGVSVDKEGYIIVADRSNNRIQVFKPCGTFHHKFGTLGSRPGQFDRPAGVACDVSRRIVVADKDNHRIQIFTFEGQFILKFGEKGTKNGQFNYPWDVAVNAEGKILVSDTRNHRVQLFGPDGVFLNKYGFEGALWKHFDSPRGVTFNHEGHLVVTDFNNHRLLVIHADCQSARFLGSEGSGNGQFLRPQGVAVDQEGRIIVADSRNHRVQIFESNGSFLCKFGAQGSGFGQMDRPSGIAVTPDGMIVVVDFGNNRILVF

Target ORF information:

RefSeq Version	XM_025147326.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tripartite motif containing 71 (TRIM71), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025147326.1

ORF Insert Sequence:

ATGGCTTCGT TCCCGGAGGC CGACTTCCAG GTGTGCCCGC TGTGCAAGGA GATGTGCGGC 
TCGCCCGCCC CGCTCTCCTC CAACTCGTCC ACCTCGTCGT CCTCGTCGCA GACCTCGGGC 
TCCTCGGGGG GCGGCGGCTC GTCGTGCGGG GGCGCGGCGC GGCGGCTGCA CGTCCTGCCC 
TGCCTGCACG CCTTCTGCCG CCAGTGCCTG GAGGCGCAGC GGCACCCCGC CGCGGGGGAC 
GCGCTCAAGC TGCGCTGCCC CATCTGCGAC CAGAAGGTGG TGATCTCGGA GCCCTCGGGC 
ATGGACGCGC TGCCCTCCTC CAACTTCCTC CTCAGCAACC TGCTGGACGT CGTGGTGGTG 
GCGGCCGCCG CCGACGAGCA GAAGAACGGC CGCGCCGCCG GCTCCGGGCC CGCCGCCGGC 
TCCGGCGCGG GGGGCGGCGG GGCCAACAAC CGCCACCACG GCCGCCCGCC GCCGCACCGC 
GCCGCCGCCG CCGCCGCCTC CTCGCCCGCC GCCGGCTCCG CCGCGCCCTC CGCCTCCTCG 
TCCTCCTCCT CGGGCGGCGG CGGCCCGGCG GCGCTGCTGC TGCGGCGGCC GCACAGCCGG 
CAGGGCGAGC CGCGCTGCAG CTCCTGCGAC GAGGGCAACG CCGCCAGCTC GCGCTGCCTG 
GACTGCCAGG AGCACCTGTG CGACAACTGC GTGCGGGCGC ACCAGCGCGT CCGCCTCACC 
AAGGACCACT TCATCGAGCG CTTCGCCGCC GGCCCCGCCG CCGCCTCCGC CGGCCCCGCC 
GCGCCGCTCG CCCTCAGCCC GCCCTACCCC GCCTCGCCCT ACAACATCCT CTCCGTCTTC 
CCCGAGCGGG CCAGCTACTG CCAGCACCAC GACGACGAGG TACTGCATTT CTACTGTGAT 
ACCTGCTCTG TCCCCATATG TCGTGAATGT ACCATGGGGC GACATGTGGG TCACAGCTTT 
ATCTACCTCC AAGATGCCCT CCAGGACTCC AGAACTCTTA CCATCCAGCT CCTGGCAGAT 
GCTCAGCAAG GACGACAGGC TATTCAGTTG AGTATTGAAC AGGCCCAGGC TGTGGCAGAG 
CAAGTTGAGA TGAAAGCGAA GGTGGTACAG TCTGAAGTCA AAGCAGTGAC TACCAGACAT 
AAGAAGGCCT TGGAAGAGCG AGAGTGCGAG CTGCTCTGGA AGGTGGAAAA GATTCGTCAA 
GTCAAGGCTA AGTCGCTCTA CTTGCAAGTT GAAAAGTTAC GTCAGAACCT AAATAAGCTG 
GACAACACTA TTAGTGCTGT TCAGCAGGTC CTGGAGGAAG GCCGTACCAT AGATATTCTT 
CTGGCCCGGG ACCGCATGCT GGCTCAGGTG CAGGAGCTCA AGAATGTGAG AGGCCTTCTG 
CAGCCACAGG AGGATGACAG GATCATGTTC ACGCCGCCTG ACCAGGCGTT GTATATGGCC 
ATTAAATCAA TGGGCTTTGT CAGTAGTGGG GCATTTGCTC CTCTGACCAA AGCCACTGGG 
GAAGGCCTCA AGCGTGCGCT GCAGGGCAAA GTAGCCTCTT TCACAGTTAT AGGCTACGAC 
CACGATGGTG AGCCTCGCCT TTCAGGAGGT GACATGATCT CTGCAGTGGT AATGGGCCCA 
GATGGAAATC TGTTTGGGGC AGACGTCAGT GATCAGCAGA ACGGAACCTA CCTGGTCAGC 
TACCGTCCGC AGCTAGAGGG AGAGCATCTG GTGTCCGTGA TGATGTGCAA CCAACACATT 
GAGAACAGCC CCTTCAAAGT TGTGGTGAAA TCTGGGCGCA GCTACATAGG TATTGGGCTA 
CCCGGGCTCT CGTTTGGCAG TGAAGGAGAC AGCGATGGGA AACTCTGCCG CCCCTGGGGG 
GTTAGCGTAG ACAAAGAAGG CTACATCATC GTGGCTGACC GCAGCAATAA CCGTATCCAG 
GTGTTCAAAC CATGTGGGAC TTTCCATCAC AAGTTTGGCA CGCTCGGTTC CCGGCCTGGC 
CAGTTCGATC GTCCCGCCGG TGTGGCCTGT GACGTCTCAC GCCGGATCGT CGTGGCTGAC 
AAGGACAATC ATCGCATCCA GATTTTCACG TTTGAGGGGC AGTTCATTCT GAAGTTTGGG 
GAGAAAGGAA CAAAGAATGG GCAATTCAAT TACCCGTGGG ACGTGGCCGT CAACGCAGAG 
GGCAAAATCC TGGTCTCCGA CACAAGGAAC CATCGCGTTC AGTTGTTCGG GCCCGATGGT 
GTGTTTCTTA ATAAGTACGG CTTTGAAGGG GCACTCTGGA AGCACTTTGA TTCCCCCAGA 
GGTGTGACTT TCAATCACGA GGGCCACTTG GTAGTGACAG ACTTCAACAA CCATCGCCTT 
CTGGTCATCC ATGCAGATTG CCAGTCAGCA CGCTTCCTGG GCTCGGAGGG GAGCGGGAAC 
GGGCAGTTCC TGCGCCCGCA GGGAGTGGCG GTGGATCAAG AGGGGCGCAT CATCGTGGCC 
GACTCCAGGA ACCACCGGGT GCAGATATTT GAGTCCAATG GTAGCTTTTT ATGCAAGTTC 
GGCGCTCAGG GAAGCGGCTT TGGTCAGATG GACCGTCCTT CAGGTATAGC TGTCACCCCT 
GATGGCATGA TTGTGGTGGT CGACTTTGGA AACAATCGAA TCCTCGTCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM71 ( XM_025147327.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025003095.1 Gallus gallus tripartite motif containing 71 (TRIM71), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa145952
Clone ID Related Accession (Same CDS sequence)	XM_025147327.1 , XM_025147328.1
Accession Version	XM_025147327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIM71 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGT TCCCGGAGGC CGACTTCCAG GTGTGCCCGC TGTGCAAGGA GATGTGCGGC 
TCGCCCGCCC CGCTCTCCTC CAACTCGTCC ACCTCGTCGT CCTCGTCGCA GACCTCGGGC 
TCCTCGGGGG GCGGCGGCTC GTCGTGCGGG GGCGCGGCGC GGCGGCTGCA CGTCCTGCCC 
TGCCTGCACG CCTTCTGCCG CCAGTGCCTG GAGGCGCAGC GGCACCCCGC CGCGGGGGAC 
GCGCTCAAGC TGCGCTGCCC CATCTGCGAC CAGAAGGTGG TGATCTCGGA GCCCTCGGGC 
ATGGACGCGC TGCCCTCCTC CAACTTCCTC CTCAGCAACC TGCTGGACGT CGTGGTGGTG 
GCGGCCGCCG CCGACGAGCA GAAGAACGGC CGCGCCGCCG GCTCCGGGCC CGCCGCCGGC 
TCCGGCGCGG GGGGCGGCGG GGCCAACAAC CGCCACCACG GCCGCCCGCC GCCGCACCGC 
GCCGCCGCCG CCGCCGCCTC CTCGCCCGCC GCCGGCTCCG CCGCGCCCTC CGCCTCCTCG 
TCCTCCTCCT CGGGCGGCGG CGGCCCGGCG GCGCTGCTGC TGCGGCGGCC GCACAGCCGG 
CAGGGCGAGC CGCGCTGCAG CTCCTGCGAC GAGGGCAACG CCGCCAGCTC GCGCTGCCTG 
GACTGCCAGG AGCACCTGTG CGACAACTGC GTGCGGGCGC ACCAGCGCGT CCGCCTCACC 
AAGGACCACT TCATCGAGCG CTTCGCCGCC GGCCCCGCCG CCGCCTCCGC CGGCCCCGCC 
GCGCCGCTCG CCCTCAGCCC GCCCTACCCC GCCTCGCCCT ACAACATCCT CTCCGTCTTC 
CCCGAGCGGG CCAGCTACTG CCAGCACCAC GACGACGAGG TACTGCATTT CTACTGTGAT 
ACCTGCTCTG TCCCCATATG TCGTGAATGT ACCATGGGGC GACATGTGGG TCACAGCTTT 
ATCTACCTCC AAGATGCCCT CCAGGACTCC AGAACTCTTA CCATCCAGCT CCTGGCAGAT 
GCTCAGCAAG GACGACAGGC TATTCAGTTG AGTATTGAAC AGGCCCAGGC TGTGGCAGAG 
CAAGTTGAGA TGAAAGCGAA GGTGGTACAG TCTGAAGTCA AAGCAGTGAC TACCAGACAT 
AAGAAGGCCT TGGAAGAGCG AGAGTGCGAG CTGCTCTGGA AGGTGGAAAA GATTCGTCAA 
GTCAAGGCTA AGTCGCTCTA CTTGCAAGTT GAAAAGTTAC GTCAGAACCT AAATAAGCTG 
GACAACACTA TTAGTGCTGT TCAGCAGGTC CTGGAGGAAG GCCGTACCAT AGATATTCTT 
CTGGCCCGGG ACCGCATGCT GGCTCAGGTG CAGGAGCTCA AGAATGTGAG AGGCCTTCTG 
CAGCCACAGG AGGATGACAG GATCATGTTC ACGCCGCCTG ACCAGGCGTT GTATATGGCC 
ATTAAATCAA TGGGCTTTGT CAGTAGTGGG GCATTTGCTC CTCTGACCAA AGCCACTGGG 
GAAGGCCTCA AGCGTGCGCT GCAGGGCAAA GTAGCCTCTT TCACAGTTAT AGGCTACGAC 
CACGATGGTG AGCCTCGCCT TTCAGGAGGT GACATGATCT CTGCAGTGGT AATGGGCCCA 
GATGGAAATC TGTTTGGGGC AGACGTCAGT GATCAGCAGA ACGGAACCTA CCTGGTCAGC 
TACCGTCCGC AGCTAGAGGG AGAGCATCTG GTGTCCGTGA TGATGTGCAA CCAACACATT 
GAGAACAGCC CCTTCAAAGT TGTGGTGAAA TCTGGGCGCA GCTACATAGG TATTGGGCTA 
CCCGGGCTCT CGTTTGGCAG TGAAGGAGAC AGCGATGGGA AACTCTGCCG CCCCTGGGGG 
GTTAGCGTAG ACAAAGAAGG CTACATCATC GTGGCTGACC GCAGCAATAA CCGTATCCAG 
GTGTTCAAAC CATGTGGGAC TTTCCATCAC AAGTTTGGCA CGCTCGGTTC CCGGCCTGGC 
CAGTTCGATC GTCCCGCCGG TGTGGCCTGT GACGTCTCAC GCCGGATCGT CGTGGCTGAC 
AAGGACAATC ATCGCATCCA GATTTTCACG TTTGAGGGGC AGTTCATTCT GAAGTTTGGG 
GAGAAAGGAA CAAAGAATGG GCAATTCAAT TACCCGTGGG ACGTGGCCGT CAACGCAGAG 
GGCAAAATCC TGGTCTCCGA CACAAGGAAC CATCGCGTTC AGTTGTTCGG GCCCGATGGT 
GTGTTTCTTA ATAAGTACGG CTTTGAAGGG GCACTCTGGA AGCACTTTGA TTCCCCCAGA 
GGTGTGACTT TCAATCACGA GGGCCACTTG GTAGTGACAG ACTTCAACAA CCATCGCCTT 
CTGGTCATCC ATGCAGATTG CCAGTCAGCA CGCTTCCTGG GCTCGGAGGG GAGCGGGAAC 
GGGCAGTTCC TGCGCCCGCA GGGAGTGGCG GTGGATCAAG AGGGGCGCAT CATCGTGGCC 
GACTCCAGGA ACCACCGGGT GCAGATATTT GAGTCCAATG GTAGCTTTTT ATGCAAGTTC 
GGCGCTCAGG GAAGCGGCTT TGGTCAGATG GACCGTCCTT CAGTGTGTTT GATTTACTGG 
CGAGCACAGC GTTTTGTTCC CAGAAGACTT TTGAACGTTT CGTGGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025003095.1
CDS	142..2772
Translation	MASFPEADFQVCPLCKEMCGSPAPLSSNSSTSSSSSQTSGSSGGGGSSCGGAARRLHVLPCLHAFCRQCLEAQRHPAAGDALKLRCPICDQKVVISEPSGMDALPSSNFLLSNLLDVVVVAAAADEQKNGRAAGSGPAAGSGAGGGGANNRHHGRPPPHRAAAAAASSPAAGSAAPSASSSSSSGGGGPAALLLRRPHSRQGEPRCSSCDEGNAASSRCLDCQEHLCDNCVRAHQRVRLTKDHFIERFAAGPAAASAGPAAPLALSPPYPASPYNILSVFPERASYCQHHDDEVLHFYCDTCSVPICRECTMGRHVGHSFIYLQDALQDSRTLTIQLLADAQQGRQAIQLSIEQAQAVAEQVEMKAKVVQSEVKAVTTRHKKALEERECELLWKVEKIRQVKAKSLYLQVEKLRQNLNKLDNTISAVQQVLEEGRTIDILLARDRMLAQVQELKNVRGLLQPQEDDRIMFTPPDQALYMAIKSMGFVSSGAFAPLTKATGEGLKRALQGKVASFTVIGYDHDGEPRLSGGDMISAVVMGPDGNLFGADVSDQQNGTYLVSYRPQLEGEHLVSVMMCNQHIENSPFKVVVKSGRSYIGIGLPGLSFGSEGDSDGKLCRPWGVSVDKEGYIIVADRSNNRIQVFKPCGTFHHKFGTLGSRPGQFDRPAGVACDVSRRIVVADKDNHRIQIFTFEGQFILKFGEKGTKNGQFNYPWDVAVNAEGKILVSDTRNHRVQLFGPDGVFLNKYGFEGALWKHFDSPRGVTFNHEGHLVVTDFNNHRLLVIHADCQSARFLGSEGSGNGQFLRPQGVAVDQEGRIIVADSRNHRVQIFESNGSFLCKFGAQGSGFGQMDRPSVCLIYWRAQRFVPRRLLNVSWK

Target ORF information:

RefSeq Version	XM_025147327.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tripartite motif containing 71 (TRIM71), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025147327.1

ORF Insert Sequence:

ATGGCTTCGT TCCCGGAGGC CGACTTCCAG GTGTGCCCGC TGTGCAAGGA GATGTGCGGC 
TCGCCCGCCC CGCTCTCCTC CAACTCGTCC ACCTCGTCGT CCTCGTCGCA GACCTCGGGC 
TCCTCGGGGG GCGGCGGCTC GTCGTGCGGG GGCGCGGCGC GGCGGCTGCA CGTCCTGCCC 
TGCCTGCACG CCTTCTGCCG CCAGTGCCTG GAGGCGCAGC GGCACCCCGC CGCGGGGGAC 
GCGCTCAAGC TGCGCTGCCC CATCTGCGAC CAGAAGGTGG TGATCTCGGA GCCCTCGGGC 
ATGGACGCGC TGCCCTCCTC CAACTTCCTC CTCAGCAACC TGCTGGACGT CGTGGTGGTG 
GCGGCCGCCG CCGACGAGCA GAAGAACGGC CGCGCCGCCG GCTCCGGGCC CGCCGCCGGC 
TCCGGCGCGG GGGGCGGCGG GGCCAACAAC CGCCACCACG GCCGCCCGCC GCCGCACCGC 
GCCGCCGCCG CCGCCGCCTC CTCGCCCGCC GCCGGCTCCG CCGCGCCCTC CGCCTCCTCG 
TCCTCCTCCT CGGGCGGCGG CGGCCCGGCG GCGCTGCTGC TGCGGCGGCC GCACAGCCGG 
CAGGGCGAGC CGCGCTGCAG CTCCTGCGAC GAGGGCAACG CCGCCAGCTC GCGCTGCCTG 
GACTGCCAGG AGCACCTGTG CGACAACTGC GTGCGGGCGC ACCAGCGCGT CCGCCTCACC 
AAGGACCACT TCATCGAGCG CTTCGCCGCC GGCCCCGCCG CCGCCTCCGC CGGCCCCGCC 
GCGCCGCTCG CCCTCAGCCC GCCCTACCCC GCCTCGCCCT ACAACATCCT CTCCGTCTTC 
CCCGAGCGGG CCAGCTACTG CCAGCACCAC GACGACGAGG TACTGCATTT CTACTGTGAT 
ACCTGCTCTG TCCCCATATG TCGTGAATGT ACCATGGGGC GACATGTGGG TCACAGCTTT 
ATCTACCTCC AAGATGCCCT CCAGGACTCC AGAACTCTTA CCATCCAGCT CCTGGCAGAT 
GCTCAGCAAG GACGACAGGC TATTCAGTTG AGTATTGAAC AGGCCCAGGC TGTGGCAGAG 
CAAGTTGAGA TGAAAGCGAA GGTGGTACAG TCTGAAGTCA AAGCAGTGAC TACCAGACAT 
AAGAAGGCCT TGGAAGAGCG AGAGTGCGAG CTGCTCTGGA AGGTGGAAAA GATTCGTCAA 
GTCAAGGCTA AGTCGCTCTA CTTGCAAGTT GAAAAGTTAC GTCAGAACCT AAATAAGCTG 
GACAACACTA TTAGTGCTGT TCAGCAGGTC CTGGAGGAAG GCCGTACCAT AGATATTCTT 
CTGGCCCGGG ACCGCATGCT GGCTCAGGTG CAGGAGCTCA AGAATGTGAG AGGCCTTCTG 
CAGCCACAGG AGGATGACAG GATCATGTTC ACGCCGCCTG ACCAGGCGTT GTATATGGCC 
ATTAAATCAA TGGGCTTTGT CAGTAGTGGG GCATTTGCTC CTCTGACCAA AGCCACTGGG 
GAAGGCCTCA AGCGTGCGCT GCAGGGCAAA GTAGCCTCTT TCACAGTTAT AGGCTACGAC 
CACGATGGTG AGCCTCGCCT TTCAGGAGGT GACATGATCT CTGCAGTGGT AATGGGCCCA 
GATGGAAATC TGTTTGGGGC AGACGTCAGT GATCAGCAGA ACGGAACCTA CCTGGTCAGC 
TACCGTCCGC AGCTAGAGGG AGAGCATCTG GTGTCCGTGA TGATGTGCAA CCAACACATT 
GAGAACAGCC CCTTCAAAGT TGTGGTGAAA TCTGGGCGCA GCTACATAGG TATTGGGCTA 
CCCGGGCTCT CGTTTGGCAG TGAAGGAGAC AGCGATGGGA AACTCTGCCG CCCCTGGGGG 
GTTAGCGTAG ACAAAGAAGG CTACATCATC GTGGCTGACC GCAGCAATAA CCGTATCCAG 
GTGTTCAAAC CATGTGGGAC TTTCCATCAC AAGTTTGGCA CGCTCGGTTC CCGGCCTGGC 
CAGTTCGATC GTCCCGCCGG TGTGGCCTGT GACGTCTCAC GCCGGATCGT CGTGGCTGAC 
AAGGACAATC ATCGCATCCA GATTTTCACG TTTGAGGGGC AGTTCATTCT GAAGTTTGGG 
GAGAAAGGAA CAAAGAATGG GCAATTCAAT TACCCGTGGG ACGTGGCCGT CAACGCAGAG 
GGCAAAATCC TGGTCTCCGA CACAAGGAAC CATCGCGTTC AGTTGTTCGG GCCCGATGGT 
GTGTTTCTTA ATAAGTACGG CTTTGAAGGG GCACTCTGGA AGCACTTTGA TTCCCCCAGA 
GGTGTGACTT TCAATCACGA GGGCCACTTG GTAGTGACAG ACTTCAACAA CCATCGCCTT 
CTGGTCATCC ATGCAGATTG CCAGTCAGCA CGCTTCCTGG GCTCGGAGGG GAGCGGGAAC 
GGGCAGTTCC TGCGCCCGCA GGGAGTGGCG GTGGATCAAG AGGGGCGCAT CATCGTGGCC 
GACTCCAGGA ACCACCGGGT GCAGATATTT GAGTCCAATG GTAGCTTTTT ATGCAAGTTC 
GGCGCTCAGG GAAGCGGCTT TGGTCAGATG GACCGTCCTT CAGTGTGTTT GATTTACTGG 
CGAGCACAGC GTTTTGTTCC CAGAAGACTT TTGAACGTTT CGTGGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM71 ( XM_025147328.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025003096.1 Gallus gallus tripartite motif containing 71 (TRIM71), transcript variant X3, mRNA.

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CloneID	OGa145952
Clone ID Related Accession (Same CDS sequence)	XM_025147327.1 , XM_025147328.1
Accession Version	XM_025147328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIM71 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGT TCCCGGAGGC CGACTTCCAG GTGTGCCCGC TGTGCAAGGA GATGTGCGGC 
TCGCCCGCCC CGCTCTCCTC CAACTCGTCC ACCTCGTCGT CCTCGTCGCA GACCTCGGGC 
TCCTCGGGGG GCGGCGGCTC GTCGTGCGGG GGCGCGGCGC GGCGGCTGCA CGTCCTGCCC 
TGCCTGCACG CCTTCTGCCG CCAGTGCCTG GAGGCGCAGC GGCACCCCGC CGCGGGGGAC 
GCGCTCAAGC TGCGCTGCCC CATCTGCGAC CAGAAGGTGG TGATCTCGGA GCCCTCGGGC 
ATGGACGCGC TGCCCTCCTC CAACTTCCTC CTCAGCAACC TGCTGGACGT CGTGGTGGTG 
GCGGCCGCCG CCGACGAGCA GAAGAACGGC CGCGCCGCCG GCTCCGGGCC CGCCGCCGGC 
TCCGGCGCGG GGGGCGGCGG GGCCAACAAC CGCCACCACG GCCGCCCGCC GCCGCACCGC 
GCCGCCGCCG CCGCCGCCTC CTCGCCCGCC GCCGGCTCCG CCGCGCCCTC CGCCTCCTCG 
TCCTCCTCCT CGGGCGGCGG CGGCCCGGCG GCGCTGCTGC TGCGGCGGCC GCACAGCCGG 
CAGGGCGAGC CGCGCTGCAG CTCCTGCGAC GAGGGCAACG CCGCCAGCTC GCGCTGCCTG 
GACTGCCAGG AGCACCTGTG CGACAACTGC GTGCGGGCGC ACCAGCGCGT CCGCCTCACC 
AAGGACCACT TCATCGAGCG CTTCGCCGCC GGCCCCGCCG CCGCCTCCGC CGGCCCCGCC 
GCGCCGCTCG CCCTCAGCCC GCCCTACCCC GCCTCGCCCT ACAACATCCT CTCCGTCTTC 
CCCGAGCGGG CCAGCTACTG CCAGCACCAC GACGACGAGG TACTGCATTT CTACTGTGAT 
ACCTGCTCTG TCCCCATATG TCGTGAATGT ACCATGGGGC GACATGTGGG TCACAGCTTT 
ATCTACCTCC AAGATGCCCT CCAGGACTCC AGAACTCTTA CCATCCAGCT CCTGGCAGAT 
GCTCAGCAAG GACGACAGGC TATTCAGTTG AGTATTGAAC AGGCCCAGGC TGTGGCAGAG 
CAAGTTGAGA TGAAAGCGAA GGTGGTACAG TCTGAAGTCA AAGCAGTGAC TACCAGACAT 
AAGAAGGCCT TGGAAGAGCG AGAGTGCGAG CTGCTCTGGA AGGTGGAAAA GATTCGTCAA 
GTCAAGGCTA AGTCGCTCTA CTTGCAAGTT GAAAAGTTAC GTCAGAACCT AAATAAGCTG 
GACAACACTA TTAGTGCTGT TCAGCAGGTC CTGGAGGAAG GCCGTACCAT AGATATTCTT 
CTGGCCCGGG ACCGCATGCT GGCTCAGGTG CAGGAGCTCA AGAATGTGAG AGGCCTTCTG 
CAGCCACAGG AGGATGACAG GATCATGTTC ACGCCGCCTG ACCAGGCGTT GTATATGGCC 
ATTAAATCAA TGGGCTTTGT CAGTAGTGGG GCATTTGCTC CTCTGACCAA AGCCACTGGG 
GAAGGCCTCA AGCGTGCGCT GCAGGGCAAA GTAGCCTCTT TCACAGTTAT AGGCTACGAC 
CACGATGGTG AGCCTCGCCT TTCAGGAGGT GACATGATCT CTGCAGTGGT AATGGGCCCA 
GATGGAAATC TGTTTGGGGC AGACGTCAGT GATCAGCAGA ACGGAACCTA CCTGGTCAGC 
TACCGTCCGC AGCTAGAGGG AGAGCATCTG GTGTCCGTGA TGATGTGCAA CCAACACATT 
GAGAACAGCC CCTTCAAAGT TGTGGTGAAA TCTGGGCGCA GCTACATAGG TATTGGGCTA 
CCCGGGCTCT CGTTTGGCAG TGAAGGAGAC AGCGATGGGA AACTCTGCCG CCCCTGGGGG 
GTTAGCGTAG ACAAAGAAGG CTACATCATC GTGGCTGACC GCAGCAATAA CCGTATCCAG 
GTGTTCAAAC CATGTGGGAC TTTCCATCAC AAGTTTGGCA CGCTCGGTTC CCGGCCTGGC 
CAGTTCGATC GTCCCGCCGG TGTGGCCTGT GACGTCTCAC GCCGGATCGT CGTGGCTGAC 
AAGGACAATC ATCGCATCCA GATTTTCACG TTTGAGGGGC AGTTCATTCT GAAGTTTGGG 
GAGAAAGGAA CAAAGAATGG GCAATTCAAT TACCCGTGGG ACGTGGCCGT CAACGCAGAG 
GGCAAAATCC TGGTCTCCGA CACAAGGAAC CATCGCGTTC AGTTGTTCGG GCCCGATGGT 
GTGTTTCTTA ATAAGTACGG CTTTGAAGGG GCACTCTGGA AGCACTTTGA TTCCCCCAGA 
GGTGTGACTT TCAATCACGA GGGCCACTTG GTAGTGACAG ACTTCAACAA CCATCGCCTT 
CTGGTCATCC ATGCAGATTG CCAGTCAGCA CGCTTCCTGG GCTCGGAGGG GAGCGGGAAC 
GGGCAGTTCC TGCGCCCGCA GGGAGTGGCG GTGGATCAAG AGGGGCGCAT CATCGTGGCC 
GACTCCAGGA ACCACCGGGT GCAGATATTT GAGTCCAATG GTAGCTTTTT ATGCAAGTTC 
GGCGCTCAGG GAAGCGGCTT TGGTCAGATG GACCGTCCTT CAGTGTGTTT GATTTACTGG 
CGAGCACAGC GTTTTGTTCC CAGAAGACTT TTGAACGTTT CGTGGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025003096.1
CDS	142..2772
Translation	MASFPEADFQVCPLCKEMCGSPAPLSSNSSTSSSSSQTSGSSGGGGSSCGGAARRLHVLPCLHAFCRQCLEAQRHPAAGDALKLRCPICDQKVVISEPSGMDALPSSNFLLSNLLDVVVVAAAADEQKNGRAAGSGPAAGSGAGGGGANNRHHGRPPPHRAAAAAASSPAAGSAAPSASSSSSSGGGGPAALLLRRPHSRQGEPRCSSCDEGNAASSRCLDCQEHLCDNCVRAHQRVRLTKDHFIERFAAGPAAASAGPAAPLALSPPYPASPYNILSVFPERASYCQHHDDEVLHFYCDTCSVPICRECTMGRHVGHSFIYLQDALQDSRTLTIQLLADAQQGRQAIQLSIEQAQAVAEQVEMKAKVVQSEVKAVTTRHKKALEERECELLWKVEKIRQVKAKSLYLQVEKLRQNLNKLDNTISAVQQVLEEGRTIDILLARDRMLAQVQELKNVRGLLQPQEDDRIMFTPPDQALYMAIKSMGFVSSGAFAPLTKATGEGLKRALQGKVASFTVIGYDHDGEPRLSGGDMISAVVMGPDGNLFGADVSDQQNGTYLVSYRPQLEGEHLVSVMMCNQHIENSPFKVVVKSGRSYIGIGLPGLSFGSEGDSDGKLCRPWGVSVDKEGYIIVADRSNNRIQVFKPCGTFHHKFGTLGSRPGQFDRPAGVACDVSRRIVVADKDNHRIQIFTFEGQFILKFGEKGTKNGQFNYPWDVAVNAEGKILVSDTRNHRVQLFGPDGVFLNKYGFEGALWKHFDSPRGVTFNHEGHLVVTDFNNHRLLVIHADCQSARFLGSEGSGNGQFLRPQGVAVDQEGRIIVADSRNHRVQIFESNGSFLCKFGAQGSGFGQMDRPSVCLIYWRAQRFVPRRLLNVSWK

Target ORF information:

RefSeq Version	XM_025147328.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tripartite motif containing 71 (TRIM71), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025147328.1

ORF Insert Sequence:

ATGGCTTCGT TCCCGGAGGC CGACTTCCAG GTGTGCCCGC TGTGCAAGGA GATGTGCGGC 
TCGCCCGCCC CGCTCTCCTC CAACTCGTCC ACCTCGTCGT CCTCGTCGCA GACCTCGGGC 
TCCTCGGGGG GCGGCGGCTC GTCGTGCGGG GGCGCGGCGC GGCGGCTGCA CGTCCTGCCC 
TGCCTGCACG CCTTCTGCCG CCAGTGCCTG GAGGCGCAGC GGCACCCCGC CGCGGGGGAC 
GCGCTCAAGC TGCGCTGCCC CATCTGCGAC CAGAAGGTGG TGATCTCGGA GCCCTCGGGC 
ATGGACGCGC TGCCCTCCTC CAACTTCCTC CTCAGCAACC TGCTGGACGT CGTGGTGGTG 
GCGGCCGCCG CCGACGAGCA GAAGAACGGC CGCGCCGCCG GCTCCGGGCC CGCCGCCGGC 
TCCGGCGCGG GGGGCGGCGG GGCCAACAAC CGCCACCACG GCCGCCCGCC GCCGCACCGC 
GCCGCCGCCG CCGCCGCCTC CTCGCCCGCC GCCGGCTCCG CCGCGCCCTC CGCCTCCTCG 
TCCTCCTCCT CGGGCGGCGG CGGCCCGGCG GCGCTGCTGC TGCGGCGGCC GCACAGCCGG 
CAGGGCGAGC CGCGCTGCAG CTCCTGCGAC GAGGGCAACG CCGCCAGCTC GCGCTGCCTG 
GACTGCCAGG AGCACCTGTG CGACAACTGC GTGCGGGCGC ACCAGCGCGT CCGCCTCACC 
AAGGACCACT TCATCGAGCG CTTCGCCGCC GGCCCCGCCG CCGCCTCCGC CGGCCCCGCC 
GCGCCGCTCG CCCTCAGCCC GCCCTACCCC GCCTCGCCCT ACAACATCCT CTCCGTCTTC 
CCCGAGCGGG CCAGCTACTG CCAGCACCAC GACGACGAGG TACTGCATTT CTACTGTGAT 
ACCTGCTCTG TCCCCATATG TCGTGAATGT ACCATGGGGC GACATGTGGG TCACAGCTTT 
ATCTACCTCC AAGATGCCCT CCAGGACTCC AGAACTCTTA CCATCCAGCT CCTGGCAGAT 
GCTCAGCAAG GACGACAGGC TATTCAGTTG AGTATTGAAC AGGCCCAGGC TGTGGCAGAG 
CAAGTTGAGA TGAAAGCGAA GGTGGTACAG TCTGAAGTCA AAGCAGTGAC TACCAGACAT 
AAGAAGGCCT TGGAAGAGCG AGAGTGCGAG CTGCTCTGGA AGGTGGAAAA GATTCGTCAA 
GTCAAGGCTA AGTCGCTCTA CTTGCAAGTT GAAAAGTTAC GTCAGAACCT AAATAAGCTG 
GACAACACTA TTAGTGCTGT TCAGCAGGTC CTGGAGGAAG GCCGTACCAT AGATATTCTT 
CTGGCCCGGG ACCGCATGCT GGCTCAGGTG CAGGAGCTCA AGAATGTGAG AGGCCTTCTG 
CAGCCACAGG AGGATGACAG GATCATGTTC ACGCCGCCTG ACCAGGCGTT GTATATGGCC 
ATTAAATCAA TGGGCTTTGT CAGTAGTGGG GCATTTGCTC CTCTGACCAA AGCCACTGGG 
GAAGGCCTCA AGCGTGCGCT GCAGGGCAAA GTAGCCTCTT TCACAGTTAT AGGCTACGAC 
CACGATGGTG AGCCTCGCCT TTCAGGAGGT GACATGATCT CTGCAGTGGT AATGGGCCCA 
GATGGAAATC TGTTTGGGGC AGACGTCAGT GATCAGCAGA ACGGAACCTA CCTGGTCAGC 
TACCGTCCGC AGCTAGAGGG AGAGCATCTG GTGTCCGTGA TGATGTGCAA CCAACACATT 
GAGAACAGCC CCTTCAAAGT TGTGGTGAAA TCTGGGCGCA GCTACATAGG TATTGGGCTA 
CCCGGGCTCT CGTTTGGCAG TGAAGGAGAC AGCGATGGGA AACTCTGCCG CCCCTGGGGG 
GTTAGCGTAG ACAAAGAAGG CTACATCATC GTGGCTGACC GCAGCAATAA CCGTATCCAG 
GTGTTCAAAC CATGTGGGAC TTTCCATCAC AAGTTTGGCA CGCTCGGTTC CCGGCCTGGC 
CAGTTCGATC GTCCCGCCGG TGTGGCCTGT GACGTCTCAC GCCGGATCGT CGTGGCTGAC 
AAGGACAATC ATCGCATCCA GATTTTCACG TTTGAGGGGC AGTTCATTCT GAAGTTTGGG 
GAGAAAGGAA CAAAGAATGG GCAATTCAAT TACCCGTGGG ACGTGGCCGT CAACGCAGAG 
GGCAAAATCC TGGTCTCCGA CACAAGGAAC CATCGCGTTC AGTTGTTCGG GCCCGATGGT 
GTGTTTCTTA ATAAGTACGG CTTTGAAGGG GCACTCTGGA AGCACTTTGA TTCCCCCAGA 
GGTGTGACTT TCAATCACGA GGGCCACTTG GTAGTGACAG ACTTCAACAA CCATCGCCTT 
CTGGTCATCC ATGCAGATTG CCAGTCAGCA CGCTTCCTGG GCTCGGAGGG GAGCGGGAAC 
GGGCAGTTCC TGCGCCCGCA GGGAGTGGCG GTGGATCAAG AGGGGCGCAT CATCGTGGCC 
GACTCCAGGA ACCACCGGGT GCAGATATTT GAGTCCAATG GTAGCTTTTT ATGCAAGTTC 
GGCGCTCAGG GAAGCGGCTT TGGTCAGATG GACCGTCCTT CAGTGTGTTT GATTTACTGG 
CGAGCACAGC GTTTTGTTCC CAGAAGACTT TTGAACGTTT CGTGGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM71 ( NM_001037275.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001032352.2 Gallus gallus tripartite motif containing 71 (TRIM71), mRNA.

Price & Availability↑

CloneID	OGa27758
Clone ID Related Accession (Same CDS sequence)	NM_001037275.2
Accession Version	NM_001037275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-10
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIM71
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ278876.1. On Feb 14, 2008 this sequence version replaced NM_001037275.1. ##Evidence-Data-START## Transcript exon combination :: DQ278876.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCTTCGT TCCCGGAGGC CGACTTCCAG GTGTGCCCGC TGTGCAAGGA GATGTGCGGC 
TCGCCCGCCC CGCTCTCCTC CAACTCGTCC ACCTCGTCGT CCTCGTCGCA GACCTCGGGC 
TCCTCGGGGG GCGGCGGCTC GTCGTGCGGG GGCGCGGCGC GGCGGCTGCA CGTCCTGCCC 
TGCCTGCACG CCTTCTGCCG CCAGTGCCTG GAGGCGCAGC GGCACCCCGC CGCGGGGGAC 
GCGCTCAAGC TGCGCTGCCC CATCTGCGAC CAGAAGGTGG TGATCTCGGA GCCCTCGGGC 
ATGGACGCGC TGCCCTCCTC CAACTTCCTC CTCAGCAACC TGCTGGACGT CGTGGTGGTG 
GCGGCCGCCG CCGACGAGCA GAAGAACGGC CGCGCCGCCG GCTCCGGGCC CGCCGCCGGC 
TCCGGCGCGG GGGGCGGCGG GGCCAACAAC CGCCACCACG GCCGCCCGCC GCCGCACCGC 
GCCGCCGCCG CCGCCTCCTC GCCCGCCGCC GGCTCCGCCG CGCCCTCCGC CTCCTCGTCC 
TCTTCCTCGG GCGGCGGCGG CCCGGCGGCG CTGCTGCTGC GGCGGCCGCA CAGCCGGCAG 
GGCGAGCCGC GCTGCAGCTC CTGCGACGAG GGCAACGCCG CCAGCTCGCG CTGCCTGGAC 
TGCCAGGAGC ACCTGTGCGA CAACTGCGTG CGGGCGCACC AGCGCGTCCG CCTCACCAAG 
GACCACTTCA TCGAGCGCTT CGCCGCCGGC CCCGCCGCCG CCTCCGCCGG CCCCGCCGCG 
CCGCTCGCCC TCAGCCCGCC CTACCCCGCC TCGCCCTACA ACATCCTCTC CGTCTTCCCC 
GAGCGGGCCA GCTACTGCCA GCACCACGAC GACGAGGTAC TGCATTTCTA CTGTGATACC 
TGCTCTGTCC CCATATGTCG TGAATGTACC ATGGGGCGAC ATGTGGGTCA CAGCTTTATC 
TACCTCCAAG ATGCCCTCCA GGACTCCAGA ACTCTTACCA TCCAGCTCCT GGCAGATGCT 
CAGCAAGGAC GACAGGCTAT TCAGTTGAGT ATTGAACAGG CCCAGGCTGT GGCAGAGCAA 
GTTGAGATGA AAGCGAAGGT GGTACAGTCT GAAGTCAAAG CAGTGACTAC CAGACATAAG 
AAGGCCTTGG AAGAGCGAGA GTGCGAGCTG CTCTGGAAGG TGGAAAAGAT TCGTCAAGTC 
AAGGCTAAGT CGCTCTACTT GCAAGTTGAA AAGTTACGTC AGAACCTAAA TAAGCTGGAC 
AACACTATTA GTGCTGTTCA GCAGGTCCTG GAGGAAGGCC GTACCATAGA TATTCTTCTG 
GCCCGGGACC GCATGCTGGC TCAGGTGCAG GAGCTCAAGA ATGTGAGAGG CCTTCTGCAG 
CCACAGGAGG ATGACAGGAT CATGTTCACG CCGCCTGACC AGGCGTTGTA TATGGCCATT 
AAATCAATGG GCTTTGTCAG TAGTGGGGCA TTTGCTCCTC TGACCAAAGC CACTGGGGAA 
GGCCTCAAGC GTGCGCTGCA GGGCAAAGTA GCCTCTTTCA CAGTTATAGG CTACGACCAC 
GATGGTGAGC CTCGCCTTTC AGGAGGTGAC ATGATCTCTG CAGTGGTAAT GGGCCCAGAT 
GGAAATCTGT TTGGGGCAGA CGTCAGTGAT CAGCAGAACG GAACCTACCT GGTCAGCTAC 
CGTCCGCAGC TAGAGGGAGA GCATCTGGTG TCCGTGATGA TGTGCAACCA ACACATTGAG 
AACAGCCCCT TCAAAGTTGT GGTGAAATCT GGGCGCAGCT ACATAGGTAT TGGGCTACCC 
GGGCTCTCGT TTGGCAGCGA AGGAGACAGC GATGGGAAAC TCTGCCGCCC CTGGGGGGTT 
AGCGTAGACA AAGAAGGCTA CATCATCGTG GCTGACCGCA GCAATAACCG TATCCAGGTG 
TTCAAACCAT GTGGGACTTT CCATCACAAG TTTGGCACGC TCGGTTCCCG GCCTGGCCAG 
TTCGATCGTC CCGCCGGTGT GGCCTGTGAC GTCTCACGCC GGATCGTCGT GGCTGACAAG 
GACAATCATC GCATCCAGAT TTTCACGTTT GAGGGGCAGT TCATTCTGAA GTTTGGGGAG 
AAAGGAACAA AGAATGGGCA ATTCAATTAC CCGTGGGACG TGGCCGTCAA CGCAGAGGGC 
AAAATCCTGG TCTCCGACAC AAGGAACCAT CGCGTTCAGT TGTTCGGGCC CGATGGTGTG 
TTTCTTAATA AGTACGGCTT TGAAGGGGCA CTCTGGAAGC ACTTTGATTC CCCCAGAGGT 
GTGACTTTCA ATCACGAGGG CCACTTGGTA GTGACAGACT TCAACAACCA TCGCCTTCTG 
GTCATCCATG CAGATTGCCA GTCAGCACGC TTCCTGGGCT CGGAGGGGAG CGGGAACGGG 
CAGTTCCTGC GCCCGCAGGG AGTGGCGGTG GATCAAGAGG GGCGCATCAT CGTGGCTGAC 
TCCAGGAACC ACCGGGTGCA GATATTTGAG TCCAATGGTA GCTTTTTATG CAAGTTCGGC 
GCTCAGGGAA GCGGCTTTGG TCAGATGGAC CGTCCTTCAG GTATAGCTGT CACCCCTGAT 
GGCATGATTG TGGTGGTCGA CTTTGGAAAC AATCGAATCC TCGTCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032352.2
CDS	450..3080
Translation	MASFPEADFQVCPLCKEMCGSPAPLSSNSSTSSSSSQTSGSSGGGGSSCGGAARRLHVLPCLHAFCRQCLEAQRHPAAGDALKLRCPICDQKVVISEPSGMDALPSSNFLLSNLLDVVVVAAAADEQKNGRAAGSGPAAGSGAGGGGANNRHHGRPPPHRAAAAASSPAAGSAAPSASSSSSSGGGGPAALLLRRPHSRQGEPRCSSCDEGNAASSRCLDCQEHLCDNCVRAHQRVRLTKDHFIERFAAGPAAASAGPAAPLALSPPYPASPYNILSVFPERASYCQHHDDEVLHFYCDTCSVPICRECTMGRHVGHSFIYLQDALQDSRTLTIQLLADAQQGRQAIQLSIEQAQAVAEQVEMKAKVVQSEVKAVTTRHKKALEERECELLWKVEKIRQVKAKSLYLQVEKLRQNLNKLDNTISAVQQVLEEGRTIDILLARDRMLAQVQELKNVRGLLQPQEDDRIMFTPPDQALYMAIKSMGFVSSGAFAPLTKATGEGLKRALQGKVASFTVIGYDHDGEPRLSGGDMISAVVMGPDGNLFGADVSDQQNGTYLVSYRPQLEGEHLVSVMMCNQHIENSPFKVVVKSGRSYIGIGLPGLSFGSEGDSDGKLCRPWGVSVDKEGYIIVADRSNNRIQVFKPCGTFHHKFGTLGSRPGQFDRPAGVACDVSRRIVVADKDNHRIQIFTFEGQFILKFGEKGTKNGQFNYPWDVAVNAEGKILVSDTRNHRVQLFGPDGVFLNKYGFEGALWKHFDSPRGVTFNHEGHLVVTDFNNHRLLVIHADCQSARFLGSEGSGNGQFLRPQGVAVDQEGRIIVADSRNHRVQIFESNGSFLCKFGAQGSGFGQMDRPSGIAVTPDGMIVVVDFGNNRILVF

Target ORF information:

RefSeq Version	NM_001037275.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tripartite motif containing 71 (TRIM71), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037275.2

ORF Insert Sequence:

ATGGCTTCGT TCCCGGAGGC CGACTTCCAG GTGTGCCCGC TGTGCAAGGA GATGTGCGGC 
TCGCCCGCCC CGCTCTCCTC CAACTCGTCC ACCTCGTCGT CCTCGTCGCA GACCTCGGGC 
TCCTCGGGGG GCGGCGGCTC GTCGTGCGGG GGCGCGGCGC GGCGGCTGCA CGTCCTGCCC 
TGCCTGCACG CCTTCTGCCG CCAGTGCCTG GAGGCGCAGC GGCACCCCGC CGCGGGGGAC 
GCGCTCAAGC TGCGCTGCCC CATCTGCGAC CAGAAGGTGG TGATCTCGGA GCCCTCGGGC 
ATGGACGCGC TGCCCTCCTC CAACTTCCTC CTCAGCAACC TGCTGGACGT CGTGGTGGTG 
GCGGCCGCCG CCGACGAGCA GAAGAACGGC CGCGCCGCCG GCTCCGGGCC CGCCGCCGGC 
TCCGGCGCGG GGGGCGGCGG GGCCAACAAC CGCCACCACG GCCGCCCGCC GCCGCACCGC 
GCCGCCGCCG CCGCCTCCTC GCCCGCCGCC GGCTCCGCCG CGCCCTCCGC CTCCTCGTCC 
TCTTCCTCGG GCGGCGGCGG CCCGGCGGCG CTGCTGCTGC GGCGGCCGCA CAGCCGGCAG 
GGCGAGCCGC GCTGCAGCTC CTGCGACGAG GGCAACGCCG CCAGCTCGCG CTGCCTGGAC 
TGCCAGGAGC ACCTGTGCGA CAACTGCGTG CGGGCGCACC AGCGCGTCCG CCTCACCAAG 
GACCACTTCA TCGAGCGCTT CGCCGCCGGC CCCGCCGCCG CCTCCGCCGG CCCCGCCGCG 
CCGCTCGCCC TCAGCCCGCC CTACCCCGCC TCGCCCTACA ACATCCTCTC CGTCTTCCCC 
GAGCGGGCCA GCTACTGCCA GCACCACGAC GACGAGGTAC TGCATTTCTA CTGTGATACC 
TGCTCTGTCC CCATATGTCG TGAATGTACC ATGGGGCGAC ATGTGGGTCA CAGCTTTATC 
TACCTCCAAG ATGCCCTCCA GGACTCCAGA ACTCTTACCA TCCAGCTCCT GGCAGATGCT 
CAGCAAGGAC GACAGGCTAT TCAGTTGAGT ATTGAACAGG CCCAGGCTGT GGCAGAGCAA 
GTTGAGATGA AAGCGAAGGT GGTACAGTCT GAAGTCAAAG CAGTGACTAC CAGACATAAG 
AAGGCCTTGG AAGAGCGAGA GTGCGAGCTG CTCTGGAAGG TGGAAAAGAT TCGTCAAGTC 
AAGGCTAAGT CGCTCTACTT GCAAGTTGAA AAGTTACGTC AGAACCTAAA TAAGCTGGAC 
AACACTATTA GTGCTGTTCA GCAGGTCCTG GAGGAAGGCC GTACCATAGA TATTCTTCTG 
GCCCGGGACC GCATGCTGGC TCAGGTGCAG GAGCTCAAGA ATGTGAGAGG CCTTCTGCAG 
CCACAGGAGG ATGACAGGAT CATGTTCACG CCGCCTGACC AGGCGTTGTA TATGGCCATT 
AAATCAATGG GCTTTGTCAG TAGTGGGGCA TTTGCTCCTC TGACCAAAGC CACTGGGGAA 
GGCCTCAAGC GTGCGCTGCA GGGCAAAGTA GCCTCTTTCA CAGTTATAGG CTACGACCAC 
GATGGTGAGC CTCGCCTTTC AGGAGGTGAC ATGATCTCTG CAGTGGTAAT GGGCCCAGAT 
GGAAATCTGT TTGGGGCAGA CGTCAGTGAT CAGCAGAACG GAACCTACCT GGTCAGCTAC 
CGTCCGCAGC TAGAGGGAGA GCATCTGGTG TCCGTGATGA TGTGCAACCA ACACATTGAG 
AACAGCCCCT TCAAAGTTGT GGTGAAATCT GGGCGCAGCT ACATAGGTAT TGGGCTACCC 
GGGCTCTCGT TTGGCAGCGA AGGAGACAGC GATGGGAAAC TCTGCCGCCC CTGGGGGGTT 
AGCGTAGACA AAGAAGGCTA CATCATCGTG GCTGACCGCA GCAATAACCG TATCCAGGTG 
TTCAAACCAT GTGGGACTTT CCATCACAAG TTTGGCACGC TCGGTTCCCG GCCTGGCCAG 
TTCGATCGTC CCGCCGGTGT GGCCTGTGAC GTCTCACGCC GGATCGTCGT GGCTGACAAG 
GACAATCATC GCATCCAGAT TTTCACGTTT GAGGGGCAGT TCATTCTGAA GTTTGGGGAG 
AAAGGAACAA AGAATGGGCA ATTCAATTAC CCGTGGGACG TGGCCGTCAA CGCAGAGGGC 
AAAATCCTGG TCTCCGACAC AAGGAACCAT CGCGTTCAGT TGTTCGGGCC CGATGGTGTG 
TTTCTTAATA AGTACGGCTT TGAAGGGGCA CTCTGGAAGC ACTTTGATTC CCCCAGAGGT 
GTGACTTTCA ATCACGAGGG CCACTTGGTA GTGACAGACT TCAACAACCA TCGCCTTCTG 
GTCATCCATG CAGATTGCCA GTCAGCACGC TTCCTGGGCT CGGAGGGGAG CGGGAACGGG 
CAGTTCCTGC GCCCGCAGGG AGTGGCGGTG GATCAAGAGG GGCGCATCAT CGTGGCTGAC 
TCCAGGAACC ACCGGGTGCA GATATTTGAG TCCAATGGTA GCTTTTTATG CAAGTTCGGC 
GCTCAGGGAA GCGGCTTTGG TCAGATGGAC CGTCCTTCAG GTATAGCTGT CACCCCTGAT 
GGCATGATTG TGGTGGTCGA CTTTGGAAAC AATCGAATCC TCGTCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cloning and regulation of the vertebrate homologue of lin-41 that functions as a heterochronic gene in Caenorhabditis elegans.
Developmental dynamics : an official publication of the American Association of Anatomists235(4)1142-9(2006 Apr)
Kanamoto T,Terada K,Yoshikawa H,Furukawa T

Reciprocal expression of lin-41 and the microRNAs let-7 and mir-125 during mouse embryogenesis.
Developmental dynamics : an official publication of the American Association of Anatomists234(4)1046-54(2005 Dec)
Schulman BR,Esquela-Kerscher A,Slack FJ

Analysis of the regulation of lin-41 during chick and mouse limb development.
Developmental dynamics : an official publication of the American Association of Anatomists234(4)948-60(2005 Dec)
Lancman JJ,Caruccio NC,Harfe BD,Pasquinelli AE,Schageman JJ,Pertsemlidis A,Fallon JF

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TRIM71 cDNA ORF clone, Gallus gallus(chicken)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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