TRIM71 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRIM71
Entrez Gene ID	131405
Full Name	tripartite motif containing 71
Synonyms	LIN-41,LIN41
General protein information	Preferred Names tripartite motif containing 71 Names E3 ubiquitin-protein ligase TRIM71 RING-type E3 ubiquitin transferase TRIM71 abnormal cell LINeage LIN-41 homolog of C. elegans Lin-41 protein lin-41 homolog tripartite motif containing 71, E3 ubiquitin protein ligase tripartite motif-containing protein 71
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p22.3
Summary	The protein encoded by this gene is an E3 ubiquitin-protein ligase that binds with miRNAs and maintains the growth and upkeep of embryonic stem cells. This gene also is involved in the G1-S phase transition of the cell cycle. [provided by RefSeq, Dec 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001039111.1	NP_001034200.1	E3 ubiquitin-protein ligase TRIM71
NM_001039111.2	NP_001034200.1	E3 ubiquitin-protein ligase TRIM71
NM_001039111.3	NP_001034200.1	E3 ubiquitin-protein ligase TRIM71

Pathways from BioSystems

Homologies

	TRIM71	XP_001098706.1
Bos taurus (cattle)	TRIM71	XP_002707823.1
Gallus gallus (chicken)	TRIM71	NP_001032352.2
Pan troglodytes (chimpanzee)	TRIM71	XP_516352.3
Rattus norvegicus (Norway rat)	Trim71	NP_001178730.1
Danio rerio (zebrafish)	trim71	XP_690252.5
Caenorhabditis elegans (roundworm)	lin-41	NP_001020998.1
Mus musculus (house mouse)	Trim71	NP_001035968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005125	XP_314006.4
Homo sapiens (human)	TRIM71	NP_001034200.1
Canis lupus familiaris (dog)	TRIM71	XP_534214.3
Drosophila melanogaster (fruit fly)	wech	NP_001137614.1
Xenopus tropicalis (tropical clawed frog)	LOC100486898	XP_004915429.1

Gene Ontology
Bibliography

Related articles in PubMed

A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis.
Treiber T, Treiber N, Plessmann U, Harlander S, Dai? JL, Eichner N, Lehmann G, Schall K, Urlaub H, Meister G
Molecular cell66(2)270-284.e13(2017 Apr)

TRIM71 suppresses tumorigenesis via modulation of Lin28B-let-7-HMGA2 signaling.
Yin J, Kim TH, Park N, Shin D, Choi HI, Cho S, Park JB, Kim JH
Oncotarget7(48)79854-79868(2016 Nov)

TRIM-NHL proteins in development and disease.
Tocchini C, Ciosk R
Seminars in cell & developmental biology47-4852-9(2015 Dec)

The ubiquitin ligase human TRIM71 regulates let-7 microRNA biogenesis via modulation of Lin28B protein.
Lee SH, Cho S, Kim MS, Choi K, Cho JY, Gwak HS, Kim YJ, Yoo H, Lee SH, Park JB, Kim JH
Biochimica et biophysica acta1839(5)374-86(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicate most-studied TRIpartite Motif (TRIM)-NHL proteins TRIM2, TRIM3, TRIM32 and TRIM71, and their mutations have been linked to diseases.
Title: TRIM-NHL proteins in development and disease.

Consistent with the let-7 microRNA stimulatory role of TRIM71 via Lin28B polyubiquitination.
Title: The ubiquitin ligase human TRIM71 regulates let-7 microRNA biogenesis via modulation of Lin28B protein.

poiopi poiopi i90iy9oyu8
Title: Exploration of deleterious single nucleotide polymorphisms in the components of human P bodies: an in silico approach.

The stem cell E3-ligase Lin-41 promotes liver cancer progression through inhibition of microRNA-mediated gene silencing.
Title: The stem cell E3-ligase Lin-41 promotes liver cancer progression through inhibition of microRNA-mediated gene silencing.

Trim71 cooperates with microRNAs to repress Cdkn1a expression and promote embryonic stem cell proliferation
Title: Trim71 cooperates with microRNAs to repress Cdkn1a expression and promote embryonic stem cell proliferation.

The following TRIM71 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRIM71 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04725	NM_001039111.3 Latest version!	Homo sapiens tripartite motif containing 71 (TRIM71), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu04725	NM_001039111.1	Homo sapiens tripartite motif containing 71, E3 ubiquitin protein ligase (TRIM71), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu04725	NM_001039111.2	Homo sapiens tripartite motif containing 71 (TRIM71), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRIM71 ( NM_001039111.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001034200.1 Homo sapiens tripartite motif containing 71, E3 ubiquitin protein ligase (TRIM71), mRNA.

Price & Availability↑

CloneID	OHu04725
Clone ID Related Accession (Same CDS sequence)	NM_001039111.1 , NM_001039111.2 , NM_001039111.3
Accession Version	NM_001039111.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	E3 ubiquitin-protein ligase TRIM71
Comment

ATGGCTTCGT TCCCCGAGAC CGATTTCCAG ATCTGCTTGC TGTGCAAGGA GATGTGCGGC 
TCGCCGGCGC CGCTCTCCTC CAACTCGTCC GCGTCGTCGT CCTCCTCGCA GACGTCCACG 
TCGTCGGGGG GCGGCGGCGG GGGCCCTGGG GCGGCGGCGC GCCGCCTACA CGTCCTGCCC 
TGCCTGCACG CCTTCTGCCG CCCCTGCCTC GAGGCGCACC GGCTGCCGGC GGCGGGCGGC 
GGCGCGGCGG GAGAGCCGCT CAAGCTGCGC TGCCCCGTGT GCGACCAGAA AGTAGTGCTA 
GCCGAGGCGG CGGGTATGGA CGCGCTGCCT TCGTCCGCCT TCCTGCTTAG CAACCTGCTC 
GACGCGGTGG TGGCCACTGC CGACGAGCCG CCGCCCAAGA ACGGGCGCGC CGGCGCTCCG 
GCGGGAGCGG GCGGCCACAG CAACCACCGG CACCACGCTC ACCACGCGCA CCCGCGCGCG 
TCCGCCTCCG CGCCGCCACT CCCGCAGGCG CCGCAGCCGC CCGCGCCTTC CCGCTCGGCA 
CCCGGCGGCC CTGCCGCTTC CCCGTCGGCG CTGCTGCTCC GCCGTCCTCA CGGCTGCAGC 
TCGTGCGATG AGGGCAACGC AGCTTCTTCG CGCTGCCTCG ACTGCCAGGA GCACCTGTGC 
GACAACTGCG TCCGAGCGCA CCAGCGCGTG CGCCTCACCA AGGACCACTA CATCGAGCGC 
GGCCCGCCGG GTCCCGGTGC CGCAGCAGCG GCGCAGCAGC TCGGGCTCGG GCCGCCCTTT 
CCCGGCCCGC CCTTCTCCAT CCTCTCAGTG TTTCCCGAGC GCCTCGGCTT CTGCCAGCAC 
CACGACGACG AGGTGCTGCA CCTGTACTGT GACACTTGCT CTGTACCCAT CTGTCGTGAG 
TGCACAATGG GCCGGCATGG GGGCCACAGC TTCATCTACC TCCAGGAGGC ACTGCAGGAC 
TCACGGGCAC TCACCATCCA GCTGCTGGCA GATGCCCAGC AGGGACGACA GGCAATCCAG 
CTGAGCATCG AGCAGGCCCA GACGGTGGCG GAACAGGTGG AGATGAAGGC GAAGGTTGTG 
CAGTCGGAGG TCAAAGCCGT GACGGCGAGG CATAAGAAAG CCCTGGAGGA ACGCGAGTGT 
GAGCTGCTGT GGAAGGTAGA AAAGATCCGC CAGGTGAAAG CCAAGTCTCT GTACCTGCAG 
GTGGAGAAGC TGCGGCAAAA CCTCAACAAG CTTGAGAGCA CCATCAGTGC CGTGCAGCAG 
GTCCTGGAGG AGGGTAGAGC GCTAGACATC CTACTGGCCC GAGACCGGAT GCTGGCCCAG 
GTGCAGGAGC TGAAGACCGT GCGGAGCCTC CTGCAGCCCC AGGAAGACGA CCGAGTCATG 
TTCACACCCC CCGATCAGGC ACTGTACCTT GCCATCAAGT CTTTTGGCTT TGTTAGCAGC 
GGGGCCTTTG CCCCACTCAC CAAGGCCACA GGCGATGGCC TCAAGCGTGC CCTCCAGGGT 
AAGGTGGCCT CCTTCACAGT CATTGGTTAT GACCACGATG GTGAGCCCCG CCTCTCAGGA 
GGCGACCTGA TGTCGGCTGT GGTCCTGGGC CCTGATGGCA ACCTGTTTGG TGCAGAGGTG 
AGTGATCAGC AGAATGGGAC ATACGTGGTG AGTTACCGAC CCCAGCTGGA GGGTGAGCAC 
CTGGTATCTG TGACACTGTG CAACCAGCAC ATTGAGAACA GCCCTTTCAA GGTGGTGGTC 
AAGTCAGGCC GCAGCTACGT GGGCATTGGG CTCCCGGGCC TGAGCTTCGG CAGTGAGGGT 
GACAGCGATG GCAAGCTCTG CCGCCCTTGG GGTGTGAGTG TAGACAAGGA GGGCTACATC 
ATTGTCGCCG ACCGCAGCAA CAACCGCATC CAGGTGTTCA AGCCCTGCGG CGCCTTCCAC 
CACAAATTCG GCACCCTGGG CTCCCGGCCT GGGCAGTTCG ACCGACCAGC CGGCGTGGCC 
TGTGACGCCT CACGCAGGAT CGTGGTGGCT GACAAGGACA ATCATCGCAT CCAGATCTTC 
ACGTTCGAGG GCCAGTTCCT CCTCAAGTTT GGTGAGAAAG GAACCAAGAA TGGGCAGTTC 
AACTACCCTT GGGATGTGGC GGTGAATTCT GAGGGCAAGA TCCTGGTCTC AGACACGAGG 
AACCACCGGA TCCAGCTGTT TGGGCCTGAT GGTGTCTTCC TAAACAAGTA TGGCTTCGAG 
GGGGCTCTCT GGAAGCACTT TGACTCCCCA CGGGGTGTGG CCTTCAACCA TGAGGGCCAC 
TTGGTGGTCA CTGACTTCAA CAACCACCGG CTCCTGGTTA TTCACCCCGA CTGCCAGTCG 
GCACGCTTTC TGGGCTCGGA GGGCACAGGC AATGGGCAGT TCCTGCGCCC ACAAGGGGTA 
GCTGTGGACC AGGAAGGGCG CATCATTGTG GCGGATTCCA GGAACCATCG GGTACAGATG 
TTTGAATCCA ACGGCAGCTT CCTGTGCAAG TTTGGTGCTC AAGGCAGCGG CTTTGGGCAG 
ATGGACCGCC CTTCCGGCAT CGCCATCACC CCCGACGGAA TGATCGTTGT GGTGGACTTT 
GGCAACAATC GAATCCTCGT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_001034200.1

Translation

MASFPETDFQICLLCKEMCGSPAPLSSNSSASSSSSQTSTSSGGGGGGPGAAARRLHVLPCLHAFCRPCLEAHRLPAAGGGAAGEPLKLRCPVCDQKVVLAEAAGMDALPSSAFLLSNLLDAVVATADEPPPKNGRAGAPAGAGGHSNHRHHAHHAHPRASASAPPLPQAPQPPAPSRSAPGGPAASPSALLLRRPHGCSSCDEGNAASSRCLDCQEHLCDNCVRAHQRVRLTKDHYIERGPPGPGAAAAAQQLGLGPPFPGPPFSILSVFPERLGFCQHHDDEVLHLYCDTCSVPICRECTMGRHGGHSFIYLQEALQDSRALTIQLLADAQQGRQAIQLSIEQAQTVAEQVEMKAKVVQSEVKAVTARHKKALEERECELLWKVEKIRQVKAKSLYLQVEKLRQNLNKLESTISAVQQVLEEGRALDILLARDRMLAQVQELKTVRSLLQPQEDDRVMFTPPDQALYLAIKSFGFVSSGAFAPLTKATGDGLKRALQGKVASFTVIGYDHDGEPRLSGGDLMSAVVLGPDGNLFGAEVSDQQNGTYVVSYRPQLEGEHLVSVTLCNQHIENSPFKVVVKSGRSYVGIGLPGLSFGSEGDSDGKLCRPWGVSVDKEGYIIVADRSNNRIQVFKPCGAFHHKFGTLGSRPGQFDRPAGVACDASRRIVVADKDNHRIQIFTFEGQFLLKFGEKGTKNGQFNYPWDVAVNSEGKILVSDTRNHRIQLFGPDGVFLNKYGFEGALWKHFDSPRGVAFNHEGHLVVTDFNNHRLLVIHPDCQSARFLGSEGTGNGQFLRPQGVAVDQEGRIIVADSRNHRVQMFESNGSFLCKFGAQGSGFGQMDRPSGIAITPDGMIVVVDFGNNRILVF

Target ORF information:

RefSeq Version	NM_001039111.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens tripartite motif containing 71, E3 ubiquitin protein ligase (TRIM71), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039111.1

ORF Insert Sequence:

ATGGCTTCGT TCCCCGAGAC CGATTTCCAG ATCTGCTTGC TGTGCAAGGA GATGTGCGGC 
TCGCCGGCGC CGCTCTCCTC CAACTCGTCC GCGTCGTCGT CCTCCTCGCA GACGTCCACG 
TCGTCGGGGG GCGGCGGCGG GGGCCCTGGG GCGGCGGCGC GCCGCCTACA CGTCCTGCCC 
TGCCTGCACG CCTTCTGCCG CCCCTGCCTC GAGGCGCACC GGCTGCCGGC GGCGGGCGGC 
GGCGCGGCGG GAGAGCCGCT CAAGCTGCGC TGCCCCGTGT GCGACCAGAA AGTAGTGCTA 
GCCGAGGCGG CGGGTATGGA CGCGCTGCCT TCGTCCGCCT TCCTGCTTAG CAACCTGCTC 
GACGCGGTGG TGGCCACTGC CGACGAGCCG CCGCCCAAGA ACGGGCGCGC CGGCGCTCCG 
GCGGGAGCGG GCGGCCACAG CAACCACCGG CACCACGCTC ACCACGCGCA CCCGCGCGCG 
TCCGCCTCCG CGCCGCCACT CCCGCAGGCG CCGCAGCCGC CCGCGCCTTC CCGCTCGGCA 
CCCGGCGGCC CTGCCGCTTC CCCGTCGGCG CTGCTGCTCC GCCGTCCTCA CGGCTGCAGC 
TCGTGCGATG AGGGCAACGC AGCTTCTTCG CGCTGCCTCG ACTGCCAGGA GCACCTGTGC 
GACAACTGCG TCCGAGCGCA CCAGCGCGTG CGCCTCACCA AGGACCACTA CATCGAGCGC 
GGCCCGCCGG GTCCCGGTGC CGCAGCAGCG GCGCAGCAGC TCGGGCTCGG GCCGCCCTTT 
CCCGGCCCGC CCTTCTCCAT CCTCTCAGTG TTTCCCGAGC GCCTCGGCTT CTGCCAGCAC 
CACGACGACG AGGTGCTGCA CCTGTACTGT GACACTTGCT CTGTACCCAT CTGTCGTGAG 
TGCACAATGG GCCGGCATGG GGGCCACAGC TTCATCTACC TCCAGGAGGC ACTGCAGGAC 
TCACGGGCAC TCACCATCCA GCTGCTGGCA GATGCCCAGC AGGGACGACA GGCAATCCAG 
CTGAGCATCG AGCAGGCCCA GACGGTGGCG GAACAGGTGG AGATGAAGGC GAAGGTTGTG 
CAGTCGGAGG TCAAAGCCGT GACGGCGAGG CATAAGAAAG CCCTGGAGGA ACGCGAGTGT 
GAGCTGCTGT GGAAGGTAGA AAAGATCCGC CAGGTGAAAG CCAAGTCTCT GTACCTGCAG 
GTGGAGAAGC TGCGGCAAAA CCTCAACAAG CTTGAGAGCA CCATCAGTGC CGTGCAGCAG 
GTCCTGGAGG AGGGTAGAGC GCTAGACATC CTACTGGCCC GAGACCGGAT GCTGGCCCAG 
GTGCAGGAGC TGAAGACCGT GCGGAGCCTC CTGCAGCCCC AGGAAGACGA CCGAGTCATG 
TTCACACCCC CCGATCAGGC ACTGTACCTT GCCATCAAGT CTTTTGGCTT TGTTAGCAGC 
GGGGCCTTTG CCCCACTCAC CAAGGCCACA GGCGATGGCC TCAAGCGTGC CCTCCAGGGT 
AAGGTGGCCT CCTTCACAGT CATTGGTTAT GACCACGATG GTGAGCCCCG CCTCTCAGGA 
GGCGACCTGA TGTCGGCTGT GGTCCTGGGC CCTGATGGCA ACCTGTTTGG TGCAGAGGTG 
AGTGATCAGC AGAATGGGAC ATACGTGGTG AGTTACCGAC CCCAGCTGGA GGGTGAGCAC 
CTGGTATCTG TGACACTGTG CAACCAGCAC ATTGAGAACA GCCCTTTCAA GGTGGTGGTC 
AAGTCAGGCC GCAGCTACGT GGGCATTGGG CTCCCGGGCC TGAGCTTCGG CAGTGAGGGT 
GACAGCGATG GCAAGCTCTG CCGCCCTTGG GGTGTGAGTG TAGACAAGGA GGGCTACATC 
ATTGTCGCCG ACCGCAGCAA CAACCGCATC CAGGTGTTCA AGCCCTGCGG CGCCTTCCAC 
CACAAATTCG GCACCCTGGG CTCCCGGCCT GGGCAGTTCG ACCGACCAGC CGGCGTGGCC 
TGTGACGCCT CACGCAGGAT CGTGGTGGCT GACAAGGACA ATCATCGCAT CCAGATCTTC 
ACGTTCGAGG GCCAGTTCCT CCTCAAGTTT GGTGAGAAAG GAACCAAGAA TGGGCAGTTC 
AACTACCCTT GGGATGTGGC GGTGAATTCT GAGGGCAAGA TCCTGGTCTC AGACACGAGG 
AACCACCGGA TCCAGCTGTT TGGGCCTGAT GGTGTCTTCC TAAACAAGTA TGGCTTCGAG 
GGGGCTCTCT GGAAGCACTT TGACTCCCCA CGGGGTGTGG CCTTCAACCA TGAGGGCCAC 
TTGGTGGTCA CTGACTTCAA CAACCACCGG CTCCTGGTTA TTCACCCCGA CTGCCAGTCG 
GCACGCTTTC TGGGCTCGGA GGGCACAGGC AATGGGCAGT TCCTGCGCCC ACAAGGGGTA 
GCTGTGGACC AGGAAGGGCG CATCATTGTG GCGGATTCCA GGAACCATCG GGTACAGATG 
TTTGAATCCA ACGGCAGCTT CCTGTGCAAG TTTGGTGCTC AAGGCAGCGG CTTTGGGCAG 
ATGGACCGCC CTTCCGGCAT CGCCATCACC CCCGACGGAA TGATCGTTGT GGTGGACTTT 
GGCAACAATC GAATCCTCGT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM71 ( NM_001039111.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001034200.1 Homo sapiens tripartite motif containing 71 (TRIM71), mRNA.

Price & Availability↑

CloneID	OHu04725
Clone ID Related Accession (Same CDS sequence)	NM_001039111.1 , NM_001039111.2 , NM_001039111.3
Accession Version	NM_001039111.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIM71
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ232881.1, AC139452.4 and AW196612.1. On Dec 22, 2015 this sequence version replaced NM_001039111.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ232881.1, DT932675.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCGT TCCCCGAGAC CGATTTCCAG ATCTGCTTGC TGTGCAAGGA GATGTGCGGC 
TCGCCGGCGC CGCTCTCCTC CAACTCGTCC GCGTCGTCGT CCTCCTCGCA GACGTCCACG 
TCGTCGGGGG GCGGCGGCGG GGGCCCTGGG GCGGCGGCGC GCCGCCTACA CGTCCTGCCC 
TGCCTGCACG CCTTCTGCCG CCCCTGCCTC GAGGCGCACC GGCTGCCGGC GGCGGGCGGC 
GGCGCGGCGG GAGAGCCGCT CAAGCTGCGC TGCCCCGTGT GCGACCAGAA AGTAGTGCTA 
GCCGAGGCGG CGGGTATGGA CGCGCTGCCT TCGTCCGCCT TCCTGCTTAG CAACCTGCTC 
GACGCGGTGG TGGCCACTGC CGACGAGCCG CCGCCCAAGA ACGGGCGCGC CGGCGCTCCG 
GCGGGAGCGG GCGGCCACAG CAACCACCGG CACCACGCTC ACCACGCGCA CCCGCGCGCG 
TCCGCCTCCG CGCCGCCACT CCCGCAGGCG CCGCAGCCGC CCGCGCCTTC CCGCTCGGCA 
CCCGGCGGCC CTGCCGCTTC CCCGTCGGCG CTGCTGCTCC GCCGTCCTCA CGGCTGCAGC 
TCGTGCGATG AGGGCAACGC AGCTTCTTCG CGCTGCCTCG ACTGCCAGGA GCACCTGTGC 
GACAACTGCG TCCGAGCGCA CCAGCGCGTG CGCCTCACCA AGGACCACTA CATCGAGCGC 
GGCCCGCCGG GTCCCGGTGC CGCAGCAGCG GCGCAGCAGC TCGGGCTCGG GCCGCCCTTT 
CCCGGCCCGC CCTTCTCCAT CCTCTCAGTG TTTCCCGAGC GCCTCGGCTT CTGCCAGCAC 
CACGACGACG AGGTGCTGCA CCTGTACTGT GACACTTGCT CTGTACCCAT CTGTCGTGAG 
TGCACAATGG GCCGGCATGG GGGCCACAGC TTCATCTACC TCCAGGAGGC ACTGCAGGAC 
TCACGGGCAC TCACCATCCA GCTGCTGGCA GATGCCCAGC AGGGACGACA GGCAATCCAG 
CTGAGCATCG AGCAGGCCCA GACGGTGGCG GAACAGGTGG AGATGAAGGC GAAGGTTGTG 
CAGTCGGAGG TCAAAGCCGT GACGGCGAGG CATAAGAAAG CCCTGGAGGA ACGCGAGTGT 
GAGCTGCTGT GGAAGGTAGA AAAGATCCGC CAGGTGAAAG CCAAGTCTCT GTACCTGCAG 
GTGGAGAAGC TGCGGCAAAA CCTCAACAAG CTTGAGAGCA CCATCAGTGC CGTGCAGCAG 
GTCCTGGAGG AGGGTAGAGC GCTAGACATC CTACTGGCCC GAGACCGGAT GCTGGCCCAG 
GTGCAGGAGC TGAAGACCGT GCGGAGCCTC CTGCAGCCCC AGGAAGACGA CCGAGTCATG 
TTCACACCCC CCGATCAGGC ACTGTACCTT GCCATCAAGT CTTTTGGCTT TGTTAGCAGC 
GGGGCCTTTG CCCCACTCAC CAAGGCCACA GGCGATGGCC TCAAGCGTGC CCTCCAGGGT 
AAGGTGGCCT CCTTCACAGT CATTGGTTAT GACCACGATG GTGAGCCCCG CCTCTCAGGA 
GGCGACCTGA TGTCGGCTGT GGTCCTGGGC CCTGATGGCA ACCTGTTTGG TGCAGAGGTG 
AGTGATCAGC AGAATGGGAC ATACGTGGTG AGTTACCGAC CCCAGCTGGA GGGTGAGCAC 
CTGGTATCTG TGACACTGTG CAACCAGCAC ATTGAGAACA GCCCTTTCAA GGTGGTGGTC 
AAGTCAGGCC GCAGCTACGT GGGCATTGGG CTCCCGGGCC TGAGCTTCGG CAGTGAGGGT 
GACAGCGATG GCAAGCTCTG CCGCCCTTGG GGTGTGAGTG TAGACAAGGA GGGCTACATC 
ATTGTCGCCG ACCGCAGCAA CAACCGCATC CAGGTGTTCA AGCCCTGCGG CGCCTTCCAC 
CACAAATTCG GCACCCTGGG CTCCCGGCCT GGGCAGTTCG ACCGACCAGC CGGCGTGGCC 
TGTGACGCCT CACGCAGGAT CGTGGTGGCT GACAAGGACA ATCATCGCAT CCAGATCTTC 
ACGTTCGAGG GCCAGTTCCT CCTCAAGTTT GGTGAGAAAG GAACCAAGAA TGGGCAGTTC 
AACTACCCTT GGGATGTGGC GGTGAATTCT GAGGGCAAGA TCCTGGTCTC AGACACGAGG 
AACCACCGGA TCCAGCTGTT TGGGCCTGAT GGTGTCTTCC TAAACAAGTA TGGCTTCGAG 
GGGGCTCTCT GGAAGCACTT TGACTCCCCA CGGGGTGTGG CCTTCAACCA TGAGGGCCAC 
TTGGTGGTCA CTGACTTCAA CAACCACCGG CTCCTGGTTA TTCACCCCGA CTGCCAGTCG 
GCACGCTTTC TGGGCTCGGA GGGCACAGGC AATGGGCAGT TCCTGCGCCC ACAAGGGGTA 
GCTGTGGACC AGGAAGGGCG CATCATTGTG GCGGATTCCA GGAACCATCG GGTACAGATG 
TTTGAATCCA ACGGCAGCTT CCTGTGCAAG TTTGGTGCTC AAGGCAGCGG CTTTGGGCAG 
ATGGACCGCC CTTCCGGCAT CGCCATCACC CCCGACGGAA TGATCGTTGT GGTGGACTTT 
GGCAACAATC GAATCCTCGT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034200.1
CDS	64..2670
Translation	MASFPETDFQICLLCKEMCGSPAPLSSNSSASSSSSQTSTSSGGGGGGPGAAARRLHVLPCLHAFCRPCLEAHRLPAAGGGAAGEPLKLRCPVCDQKVVLAEAAGMDALPSSAFLLSNLLDAVVATADEPPPKNGRAGAPAGAGGHSNHRHHAHHAHPRASASAPPLPQAPQPPAPSRSAPGGPAASPSALLLRRPHGCSSCDEGNAASSRCLDCQEHLCDNCVRAHQRVRLTKDHYIERGPPGPGAAAAAQQLGLGPPFPGPPFSILSVFPERLGFCQHHDDEVLHLYCDTCSVPICRECTMGRHGGHSFIYLQEALQDSRALTIQLLADAQQGRQAIQLSIEQAQTVAEQVEMKAKVVQSEVKAVTARHKKALEERECELLWKVEKIRQVKAKSLYLQVEKLRQNLNKLESTISAVQQVLEEGRALDILLARDRMLAQVQELKTVRSLLQPQEDDRVMFTPPDQALYLAIKSFGFVSSGAFAPLTKATGDGLKRALQGKVASFTVIGYDHDGEPRLSGGDLMSAVVLGPDGNLFGAEVSDQQNGTYVVSYRPQLEGEHLVSVTLCNQHIENSPFKVVVKSGRSYVGIGLPGLSFGSEGDSDGKLCRPWGVSVDKEGYIIVADRSNNRIQVFKPCGAFHHKFGTLGSRPGQFDRPAGVACDASRRIVVADKDNHRIQIFTFEGQFLLKFGEKGTKNGQFNYPWDVAVNSEGKILVSDTRNHRIQLFGPDGVFLNKYGFEGALWKHFDSPRGVAFNHEGHLVVTDFNNHRLLVIHPDCQSARFLGSEGTGNGQFLRPQGVAVDQEGRIIVADSRNHRVQMFESNGSFLCKFGAQGSGFGQMDRPSGIAITPDGMIVVVDFGNNRILVF

Target ORF information:

RefSeq Version	NM_001039111.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 71 (TRIM71), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039111.2

ORF Insert Sequence:

ATGGCTTCGT TCCCCGAGAC CGATTTCCAG ATCTGCTTGC TGTGCAAGGA GATGTGCGGC 
TCGCCGGCGC CGCTCTCCTC CAACTCGTCC GCGTCGTCGT CCTCCTCGCA GACGTCCACG 
TCGTCGGGGG GCGGCGGCGG GGGCCCTGGG GCGGCGGCGC GCCGCCTACA CGTCCTGCCC 
TGCCTGCACG CCTTCTGCCG CCCCTGCCTC GAGGCGCACC GGCTGCCGGC GGCGGGCGGC 
GGCGCGGCGG GAGAGCCGCT CAAGCTGCGC TGCCCCGTGT GCGACCAGAA AGTAGTGCTA 
GCCGAGGCGG CGGGTATGGA CGCGCTGCCT TCGTCCGCCT TCCTGCTTAG CAACCTGCTC 
GACGCGGTGG TGGCCACTGC CGACGAGCCG CCGCCCAAGA ACGGGCGCGC CGGCGCTCCG 
GCGGGAGCGG GCGGCCACAG CAACCACCGG CACCACGCTC ACCACGCGCA CCCGCGCGCG 
TCCGCCTCCG CGCCGCCACT CCCGCAGGCG CCGCAGCCGC CCGCGCCTTC CCGCTCGGCA 
CCCGGCGGCC CTGCCGCTTC CCCGTCGGCG CTGCTGCTCC GCCGTCCTCA CGGCTGCAGC 
TCGTGCGATG AGGGCAACGC AGCTTCTTCG CGCTGCCTCG ACTGCCAGGA GCACCTGTGC 
GACAACTGCG TCCGAGCGCA CCAGCGCGTG CGCCTCACCA AGGACCACTA CATCGAGCGC 
GGCCCGCCGG GTCCCGGTGC CGCAGCAGCG GCGCAGCAGC TCGGGCTCGG GCCGCCCTTT 
CCCGGCCCGC CCTTCTCCAT CCTCTCAGTG TTTCCCGAGC GCCTCGGCTT CTGCCAGCAC 
CACGACGACG AGGTGCTGCA CCTGTACTGT GACACTTGCT CTGTACCCAT CTGTCGTGAG 
TGCACAATGG GCCGGCATGG GGGCCACAGC TTCATCTACC TCCAGGAGGC ACTGCAGGAC 
TCACGGGCAC TCACCATCCA GCTGCTGGCA GATGCCCAGC AGGGACGACA GGCAATCCAG 
CTGAGCATCG AGCAGGCCCA GACGGTGGCG GAACAGGTGG AGATGAAGGC GAAGGTTGTG 
CAGTCGGAGG TCAAAGCCGT GACGGCGAGG CATAAGAAAG CCCTGGAGGA ACGCGAGTGT 
GAGCTGCTGT GGAAGGTAGA AAAGATCCGC CAGGTGAAAG CCAAGTCTCT GTACCTGCAG 
GTGGAGAAGC TGCGGCAAAA CCTCAACAAG CTTGAGAGCA CCATCAGTGC CGTGCAGCAG 
GTCCTGGAGG AGGGTAGAGC GCTAGACATC CTACTGGCCC GAGACCGGAT GCTGGCCCAG 
GTGCAGGAGC TGAAGACCGT GCGGAGCCTC CTGCAGCCCC AGGAAGACGA CCGAGTCATG 
TTCACACCCC CCGATCAGGC ACTGTACCTT GCCATCAAGT CTTTTGGCTT TGTTAGCAGC 
GGGGCCTTTG CCCCACTCAC CAAGGCCACA GGCGATGGCC TCAAGCGTGC CCTCCAGGGT 
AAGGTGGCCT CCTTCACAGT CATTGGTTAT GACCACGATG GTGAGCCCCG CCTCTCAGGA 
GGCGACCTGA TGTCGGCTGT GGTCCTGGGC CCTGATGGCA ACCTGTTTGG TGCAGAGGTG 
AGTGATCAGC AGAATGGGAC ATACGTGGTG AGTTACCGAC CCCAGCTGGA GGGTGAGCAC 
CTGGTATCTG TGACACTGTG CAACCAGCAC ATTGAGAACA GCCCTTTCAA GGTGGTGGTC 
AAGTCAGGCC GCAGCTACGT GGGCATTGGG CTCCCGGGCC TGAGCTTCGG CAGTGAGGGT 
GACAGCGATG GCAAGCTCTG CCGCCCTTGG GGTGTGAGTG TAGACAAGGA GGGCTACATC 
ATTGTCGCCG ACCGCAGCAA CAACCGCATC CAGGTGTTCA AGCCCTGCGG CGCCTTCCAC 
CACAAATTCG GCACCCTGGG CTCCCGGCCT GGGCAGTTCG ACCGACCAGC CGGCGTGGCC 
TGTGACGCCT CACGCAGGAT CGTGGTGGCT GACAAGGACA ATCATCGCAT CCAGATCTTC 
ACGTTCGAGG GCCAGTTCCT CCTCAAGTTT GGTGAGAAAG GAACCAAGAA TGGGCAGTTC 
AACTACCCTT GGGATGTGGC GGTGAATTCT GAGGGCAAGA TCCTGGTCTC AGACACGAGG 
AACCACCGGA TCCAGCTGTT TGGGCCTGAT GGTGTCTTCC TAAACAAGTA TGGCTTCGAG 
GGGGCTCTCT GGAAGCACTT TGACTCCCCA CGGGGTGTGG CCTTCAACCA TGAGGGCCAC 
TTGGTGGTCA CTGACTTCAA CAACCACCGG CTCCTGGTTA TTCACCCCGA CTGCCAGTCG 
GCACGCTTTC TGGGCTCGGA GGGCACAGGC AATGGGCAGT TCCTGCGCCC ACAAGGGGTA 
GCTGTGGACC AGGAAGGGCG CATCATTGTG GCGGATTCCA GGAACCATCG GGTACAGATG 
TTTGAATCCA ACGGCAGCTT CCTGTGCAAG TTTGGTGCTC AAGGCAGCGG CTTTGGGCAG 
ATGGACCGCC CTTCCGGCAT CGCCATCACC CCCGACGGAA TGATCGTTGT GGTGGACTTT 
GGCAACAATC GAATCCTCGT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM71 ( NM_001039111.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001034200.1 Homo sapiens tripartite motif containing 71 (TRIM71), mRNA.

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CloneID	OHu04725
Clone ID Related Accession (Same CDS sequence)	NM_001039111.1 , NM_001039111.2 , NM_001039111.3
Accession Version	NM_001039111.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-24
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIM71
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC139452.4, DQ232881.1 and AW196612.1. On Nov 22, 2018 this sequence version replaced NM_001039111.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ232881.1, DT932675.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000383763.6/ ENSP00000373272.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTTCGT TCCCCGAGAC CGATTTCCAG ATCTGCTTGC TGTGCAAGGA GATGTGCGGC 
TCGCCGGCGC CGCTCTCCTC CAACTCGTCC GCGTCGTCGT CCTCCTCGCA GACGTCCACG 
TCGTCGGGGG GCGGCGGCGG GGGCCCTGGG GCGGCGGCGC GCCGCCTACA CGTCCTGCCC 
TGCCTGCACG CCTTCTGCCG CCCCTGCCTC GAGGCGCACC GGCTGCCGGC GGCGGGCGGC 
GGCGCGGCGG GAGAGCCGCT CAAGCTGCGC TGCCCCGTGT GCGACCAGAA AGTAGTGCTA 
GCCGAGGCGG CGGGTATGGA CGCGCTGCCT TCGTCCGCCT TCCTGCTTAG CAACCTGCTC 
GACGCGGTGG TGGCCACTGC CGACGAGCCG CCGCCCAAGA ACGGGCGCGC CGGCGCTCCG 
GCGGGAGCGG GCGGCCACAG CAACCACCGG CACCACGCTC ACCACGCGCA CCCGCGCGCG 
TCCGCCTCCG CGCCGCCACT CCCGCAGGCG CCGCAGCCGC CCGCGCCTTC CCGCTCGGCA 
CCCGGCGGCC CTGCCGCTTC CCCGTCGGCG CTGCTGCTCC GCCGTCCTCA CGGCTGCAGC 
TCGTGCGATG AGGGCAACGC AGCTTCTTCG CGCTGCCTCG ACTGCCAGGA GCACCTGTGC 
GACAACTGCG TCCGAGCGCA CCAGCGCGTG CGCCTCACCA AGGACCACTA CATCGAGCGC 
GGCCCGCCGG GTCCCGGTGC CGCAGCAGCG GCGCAGCAGC TCGGGCTCGG GCCGCCCTTT 
CCCGGCCCGC CCTTCTCCAT CCTCTCAGTG TTTCCCGAGC GCCTCGGCTT CTGCCAGCAC 
CACGACGACG AGGTGCTGCA CCTGTACTGT GACACTTGCT CTGTACCCAT CTGTCGTGAG 
TGCACAATGG GCCGGCATGG GGGCCACAGC TTCATCTACC TCCAGGAGGC ACTGCAGGAC 
TCACGGGCAC TCACCATCCA GCTGCTGGCA GATGCCCAGC AGGGACGACA GGCAATCCAG 
CTGAGCATCG AGCAGGCCCA GACGGTGGCG GAACAGGTGG AGATGAAGGC GAAGGTTGTG 
CAGTCGGAGG TCAAAGCCGT GACGGCGAGG CATAAGAAAG CCCTGGAGGA ACGCGAGTGT 
GAGCTGCTGT GGAAGGTAGA AAAGATCCGC CAGGTGAAAG CCAAGTCTCT GTACCTGCAG 
GTGGAGAAGC TGCGGCAAAA CCTCAACAAG CTTGAGAGCA CCATCAGTGC CGTGCAGCAG 
GTCCTGGAGG AGGGTAGAGC GCTAGACATC CTACTGGCCC GAGACCGGAT GCTGGCCCAG 
GTGCAGGAGC TGAAGACCGT GCGGAGCCTC CTGCAGCCCC AGGAAGACGA CCGAGTCATG 
TTCACACCCC CCGATCAGGC ACTGTACCTT GCCATCAAGT CTTTTGGCTT TGTTAGCAGC 
GGGGCCTTTG CCCCACTCAC CAAGGCCACA GGCGATGGCC TCAAGCGTGC CCTCCAGGGT 
AAGGTGGCCT CCTTCACAGT CATTGGTTAT GACCACGATG GTGAGCCCCG CCTCTCAGGA 
GGCGACCTGA TGTCGGCTGT GGTCCTGGGC CCTGATGGCA ACCTGTTTGG TGCAGAGGTG 
AGTGATCAGC AGAATGGGAC ATACGTGGTG AGTTACCGAC CCCAGCTGGA GGGTGAGCAC 
CTGGTATCTG TGACACTGTG CAACCAGCAC ATTGAGAACA GCCCTTTCAA GGTGGTGGTC 
AAGTCAGGCC GCAGCTACGT GGGCATTGGG CTCCCGGGCC TGAGCTTCGG CAGTGAGGGT 
GACAGCGATG GCAAGCTCTG CCGCCCTTGG GGTGTGAGTG TAGACAAGGA GGGCTACATC 
ATTGTCGCCG ACCGCAGCAA CAACCGCATC CAGGTGTTCA AGCCCTGCGG CGCCTTCCAC 
CACAAATTCG GCACCCTGGG CTCCCGGCCT GGGCAGTTCG ACCGACCAGC CGGCGTGGCC 
TGTGACGCCT CACGCAGGAT CGTGGTGGCT GACAAGGACA ATCATCGCAT CCAGATCTTC 
ACGTTCGAGG GCCAGTTCCT CCTCAAGTTT GGTGAGAAAG GAACCAAGAA TGGGCAGTTC 
AACTACCCTT GGGATGTGGC GGTGAATTCT GAGGGCAAGA TCCTGGTCTC AGACACGAGG 
AACCACCGGA TCCAGCTGTT TGGGCCTGAT GGTGTCTTCC TAAACAAGTA TGGCTTCGAG 
GGGGCTCTCT GGAAGCACTT TGACTCCCCA CGGGGTGTGG CCTTCAACCA TGAGGGCCAC 
TTGGTGGTCA CTGACTTCAA CAACCACCGG CTCCTGGTTA TTCACCCCGA CTGCCAGTCG 
GCACGCTTTC TGGGCTCGGA GGGCACAGGC AATGGGCAGT TCCTGCGCCC ACAAGGGGTA 
GCTGTGGACC AGGAAGGGCG CATCATTGTG GCGGATTCCA GGAACCATCG GGTACAGATG 
TTTGAATCCA ACGGCAGCTT CCTGTGCAAG TTTGGTGCTC AAGGCAGCGG CTTTGGGCAG 
ATGGACCGCC CTTCCGGCAT CGCCATCACC CCCGACGGAA TGATCGTTGT GGTGGACTTT 
GGCAACAATC GAATCCTCGT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034200.1
CDS	85..2691
Translation	MASFPETDFQICLLCKEMCGSPAPLSSNSSASSSSSQTSTSSGGGGGGPGAAARRLHVLPCLHAFCRPCLEAHRLPAAGGGAAGEPLKLRCPVCDQKVVLAEAAGMDALPSSAFLLSNLLDAVVATADEPPPKNGRAGAPAGAGGHSNHRHHAHHAHPRASASAPPLPQAPQPPAPSRSAPGGPAASPSALLLRRPHGCSSCDEGNAASSRCLDCQEHLCDNCVRAHQRVRLTKDHYIERGPPGPGAAAAAQQLGLGPPFPGPPFSILSVFPERLGFCQHHDDEVLHLYCDTCSVPICRECTMGRHGGHSFIYLQEALQDSRALTIQLLADAQQGRQAIQLSIEQAQTVAEQVEMKAKVVQSEVKAVTARHKKALEERECELLWKVEKIRQVKAKSLYLQVEKLRQNLNKLESTISAVQQVLEEGRALDILLARDRMLAQVQELKTVRSLLQPQEDDRVMFTPPDQALYLAIKSFGFVSSGAFAPLTKATGDGLKRALQGKVASFTVIGYDHDGEPRLSGGDLMSAVVLGPDGNLFGAEVSDQQNGTYVVSYRPQLEGEHLVSVTLCNQHIENSPFKVVVKSGRSYVGIGLPGLSFGSEGDSDGKLCRPWGVSVDKEGYIIVADRSNNRIQVFKPCGAFHHKFGTLGSRPGQFDRPAGVACDASRRIVVADKDNHRIQIFTFEGQFLLKFGEKGTKNGQFNYPWDVAVNSEGKILVSDTRNHRIQLFGPDGVFLNKYGFEGALWKHFDSPRGVAFNHEGHLVVTDFNNHRLLVIHPDCQSARFLGSEGTGNGQFLRPQGVAVDQEGRIIVADSRNHRVQMFESNGSFLCKFGAQGSGFGQMDRPSGIAITPDGMIVVVDFGNNRILVF

Target ORF information:

RefSeq Version	NM_001039111.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 71 (TRIM71), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039111.3

ORF Insert Sequence:

ATGGCTTCGT TCCCCGAGAC CGATTTCCAG ATCTGCTTGC TGTGCAAGGA GATGTGCGGC 
TCGCCGGCGC CGCTCTCCTC CAACTCGTCC GCGTCGTCGT CCTCCTCGCA GACGTCCACG 
TCGTCGGGGG GCGGCGGCGG GGGCCCTGGG GCGGCGGCGC GCCGCCTACA CGTCCTGCCC 
TGCCTGCACG CCTTCTGCCG CCCCTGCCTC GAGGCGCACC GGCTGCCGGC GGCGGGCGGC 
GGCGCGGCGG GAGAGCCGCT CAAGCTGCGC TGCCCCGTGT GCGACCAGAA AGTAGTGCTA 
GCCGAGGCGG CGGGTATGGA CGCGCTGCCT TCGTCCGCCT TCCTGCTTAG CAACCTGCTC 
GACGCGGTGG TGGCCACTGC CGACGAGCCG CCGCCCAAGA ACGGGCGCGC CGGCGCTCCG 
GCGGGAGCGG GCGGCCACAG CAACCACCGG CACCACGCTC ACCACGCGCA CCCGCGCGCG 
TCCGCCTCCG CGCCGCCACT CCCGCAGGCG CCGCAGCCGC CCGCGCCTTC CCGCTCGGCA 
CCCGGCGGCC CTGCCGCTTC CCCGTCGGCG CTGCTGCTCC GCCGTCCTCA CGGCTGCAGC 
TCGTGCGATG AGGGCAACGC AGCTTCTTCG CGCTGCCTCG ACTGCCAGGA GCACCTGTGC 
GACAACTGCG TCCGAGCGCA CCAGCGCGTG CGCCTCACCA AGGACCACTA CATCGAGCGC 
GGCCCGCCGG GTCCCGGTGC CGCAGCAGCG GCGCAGCAGC TCGGGCTCGG GCCGCCCTTT 
CCCGGCCCGC CCTTCTCCAT CCTCTCAGTG TTTCCCGAGC GCCTCGGCTT CTGCCAGCAC 
CACGACGACG AGGTGCTGCA CCTGTACTGT GACACTTGCT CTGTACCCAT CTGTCGTGAG 
TGCACAATGG GCCGGCATGG GGGCCACAGC TTCATCTACC TCCAGGAGGC ACTGCAGGAC 
TCACGGGCAC TCACCATCCA GCTGCTGGCA GATGCCCAGC AGGGACGACA GGCAATCCAG 
CTGAGCATCG AGCAGGCCCA GACGGTGGCG GAACAGGTGG AGATGAAGGC GAAGGTTGTG 
CAGTCGGAGG TCAAAGCCGT GACGGCGAGG CATAAGAAAG CCCTGGAGGA ACGCGAGTGT 
GAGCTGCTGT GGAAGGTAGA AAAGATCCGC CAGGTGAAAG CCAAGTCTCT GTACCTGCAG 
GTGGAGAAGC TGCGGCAAAA CCTCAACAAG CTTGAGAGCA CCATCAGTGC CGTGCAGCAG 
GTCCTGGAGG AGGGTAGAGC GCTAGACATC CTACTGGCCC GAGACCGGAT GCTGGCCCAG 
GTGCAGGAGC TGAAGACCGT GCGGAGCCTC CTGCAGCCCC AGGAAGACGA CCGAGTCATG 
TTCACACCCC CCGATCAGGC ACTGTACCTT GCCATCAAGT CTTTTGGCTT TGTTAGCAGC 
GGGGCCTTTG CCCCACTCAC CAAGGCCACA GGCGATGGCC TCAAGCGTGC CCTCCAGGGT 
AAGGTGGCCT CCTTCACAGT CATTGGTTAT GACCACGATG GTGAGCCCCG CCTCTCAGGA 
GGCGACCTGA TGTCGGCTGT GGTCCTGGGC CCTGATGGCA ACCTGTTTGG TGCAGAGGTG 
AGTGATCAGC AGAATGGGAC ATACGTGGTG AGTTACCGAC CCCAGCTGGA GGGTGAGCAC 
CTGGTATCTG TGACACTGTG CAACCAGCAC ATTGAGAACA GCCCTTTCAA GGTGGTGGTC 
AAGTCAGGCC GCAGCTACGT GGGCATTGGG CTCCCGGGCC TGAGCTTCGG CAGTGAGGGT 
GACAGCGATG GCAAGCTCTG CCGCCCTTGG GGTGTGAGTG TAGACAAGGA GGGCTACATC 
ATTGTCGCCG ACCGCAGCAA CAACCGCATC CAGGTGTTCA AGCCCTGCGG CGCCTTCCAC 
CACAAATTCG GCACCCTGGG CTCCCGGCCT GGGCAGTTCG ACCGACCAGC CGGCGTGGCC 
TGTGACGCCT CACGCAGGAT CGTGGTGGCT GACAAGGACA ATCATCGCAT CCAGATCTTC 
ACGTTCGAGG GCCAGTTCCT CCTCAAGTTT GGTGAGAAAG GAACCAAGAA TGGGCAGTTC 
AACTACCCTT GGGATGTGGC GGTGAATTCT GAGGGCAAGA TCCTGGTCTC AGACACGAGG 
AACCACCGGA TCCAGCTGTT TGGGCCTGAT GGTGTCTTCC TAAACAAGTA TGGCTTCGAG 
GGGGCTCTCT GGAAGCACTT TGACTCCCCA CGGGGTGTGG CCTTCAACCA TGAGGGCCAC 
TTGGTGGTCA CTGACTTCAA CAACCACCGG CTCCTGGTTA TTCACCCCGA CTGCCAGTCG 
GCACGCTTTC TGGGCTCGGA GGGCACAGGC AATGGGCAGT TCCTGCGCCC ACAAGGGGTA 
GCTGTGGACC AGGAAGGGCG CATCATTGTG GCGGATTCCA GGAACCATCG GGTACAGATG 
TTTGAATCCA ACGGCAGCTT CCTGTGCAAG TTTGGTGCTC AAGGCAGCGG CTTTGGGCAG 
ATGGACCGCC CTTCCGGCAT CGCCATCACC CCCGACGGAA TGATCGTTGT GGTGGACTTT 
GGCAACAATC GAATCCTCGT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis.
Molecular cell66(2)270-284.e13(2017 Apr)
Treiber T,Treiber N,Plessmann U,Harlander S,Dai? JL,Eichner N,Lehmann G,Schall K,Urlaub H,Meister G

TRIM71 suppresses tumorigenesis via modulation of Lin28B-let-7-HMGA2 signaling.
Oncotarget7(48)79854-79868(2016 Nov)
Yin J,Kim TH,Park N,Shin D,Choi HI,Cho S,Park JB,Kim JH

TRIM-NHL proteins in development and disease.
Seminars in cell & developmental biology47-4852-9(2015 Dec)
Tocchini C,Ciosk R

The ubiquitin ligase human TRIM71 regulates let-7 microRNA biogenesis via modulation of Lin28B protein.
Biochimica et biophysica acta1839(5)374-86(2014 May)
Lee SH,Cho S,Kim MS,Choi K,Cho JY,Gwak HS,Kim YJ,Yoo H,Lee SH,Park JB,Kim JH

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TRIM71 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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