trim71 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	trim71
Entrez Gene ID	100486898
Full Name	tripartite motif containing 71, E3 ubiquitin protein ligase
General protein information	Preferred Names tripartite motif containing 71, E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase TRIM71
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004915373.4	XP_004915430.1	E3 ubiquitin-protein ligase TRIM71 isoform X2
XM_002937846.4	XP_002937892.1	E3 ubiquitin-protein ligase TRIM71 isoform X3
XM_004915372.3	XP_004915429.1	E3 ubiquitin-protein ligase TRIM71 isoform X1
XM_004915373.3	XP_004915430.1	E3 ubiquitin-protein ligase TRIM71 isoform X2
XM_004915372.4	XP_004915429.1	E3 ubiquitin-protein ligase TRIM71 isoform X1
XM_002937846.5	XP_002937892.1	E3 ubiquitin-protein ligase TRIM71 isoform X3

Pathways from BioSystems

Homologies

	TRIM71	XP_001098706.1
Bos taurus (cattle)	TRIM71	XP_002707823.1
Gallus gallus (chicken)	TRIM71	NP_001032352.2
Pan troglodytes (chimpanzee)	TRIM71	XP_516352.3
Rattus norvegicus (Norway rat)	Trim71	NP_001178730.1
Danio rerio (zebrafish)	trim71	XP_690252.5
Caenorhabditis elegans (roundworm)	lin-41	NP_001020998.1
Mus musculus (house mouse)	Trim71	NP_001035968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005125	XP_314006.4
Homo sapiens (human)	TRIM71	NP_001034200.1
Canis lupus familiaris (dog)	TRIM71	XP_534214.3
Drosophila melanogaster (fruit fly)	wech	NP_001137614.1
Xenopus tropicalis (tropical clawed frog)	LOC100486898	XP_004915429.1

Gene Ontology

The following trim71 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trim71 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa11890	XM_004915372.3 Latest version!	Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa11891	XM_004915373.3 Latest version!	Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa11892	XM_002937846.4 Latest version!	Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa60085	XM_004915372.4 Latest version!	Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa60086	XM_004915373.4 Latest version!	Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa60087	XM_002937846.5 Latest version!	Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trim71 ( XM_004915372.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915429.1 Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa11890
Clone ID Related Accession (Same CDS sequence)	XM_004915372.3
Accession Version	XM_004915372.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase TRIM71 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915372.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCAT TCCCGGAGTC CGATTTCCAG CTGTGCCCCC TGTGTAAGGA GATGTGCGTG 
TCCTCTGCCC CCATCTCCTC CTCCAGCTCT TCCTCATCCT CCTCCCAGGT GTCCACCAGC 
AGCAGTAGCG CCGGCAGTGC AGGAGGAGGC ACCGGCTTGG CCTCGGCTCC CCCCCGCAGG 
CTGCACGTCT TGCCGTGCCT GCACGCCTTC TGTCGCCAGT GCCTGGAGGG GCGCCGGAGC 
CCCGGGGACT CTCTCCAGCT GCGCTGCCCG GTCTGTGACC ACAAGGTGCT CATCTCCGAG 
GCCGGCATGG ACGCGCTGCC CTCTTCTACC TTCTTGCACC TCAGCAACCT GCTGGACGCG 
GTGGTGGGCG CAGCCGACGA GCAGCAACAG AGTAACGGTG GCCGGACTGC TTCCAACAGA 
CAGCGTTCGG CTTCATGTTC TTCCTCCGGC CTCCTCCGCA GGGCGCCCCC CAGTCAGTCC 
GAACCGCGGT GCTCCTCATG CGACGATGGC AACGGGGCCA GCTCGCACTG TTTGGACTGC 
CAGGAGAACT TGTGCGACAA CTGCCTGAGG GCCCACCAGC GGGTGCGACT TACTAAGGAC 
CACTTCATAG AGCGCTTCCC CGCCTCCCCC TGCAGCTCCG CGGCTTCCAG TGCCGCCACT 
ACCTCGTCCT CTTCCAGCTC GGCCTTCTCC CTCCTGCCTG TGTACCCGGA GAGGCTCTAC 
TGCCAGCAGC ACGATGAGGA GATTTACAAA TTTGGAATAC GCAAGAGAGT GTATACCCTG 
GCGGATCTGG TGAGGATTAA GGATTGGGTC CTGCATTTCT ACTGTGACTC CTGCTCTGTT 
CCTATATGTC GTGAGTGCAC TATGGGCCGT CATGCTGGAC ACAGCTTTGT CTACCTCCAG 
GAAGCTCTTC AGGACTCAAG GGCACTAACT ATTCAGCTAT TAGCAGATGC CCAACAAGGC 
CGTCAAGCAA TTCAGCTGAG TCTAGAACAA GCTCAAGCAT TAGCAGAACA AGTTGAGATG 
AAAGCCAAAG TGGTGCAGTC TGAGATCAAA GCAGTCACAT CTAGACACAA GAAGGCATTG 
GAAGAGAGAG AGTGTGAGCT GCTCTGGAAG GTTGAAAAGA TACGTCAGGT GAAAGCAAAA 
TCACTTTATC ACCAAGTTGA AAAACTCCAC CAGGCCCTTA ATAAATTGGA CAGCACAATA 
AATGCAGTGC AGCAAGTCTT GGAGGAAGGT TGTACTATGG ATATCCTGAT AGCTCGTGAT 
CGTGTTTTGG CTCAGGTGCA GGATGTAAAG AATGCTAGAG GCCTTTTACA ACTTCAAGAA 
GATGACAGGA TTATGTTTAC TCCTCCTGAC CAAGCCTTAT ACCTTGCCAT CAAATCCTTG 
GGTCTTGTCA GCAGTGGAGC TTTTGCAGCT TTGACTAAAG CCACTGGAGA AGGACTAAAG 
CGTGCCCTAC AGGGAAAAGT TGCATCATTC ACTGTTATAG GATATGACCA TGATGGAGAA 
GCCCGCCTCT CAGGTGGGGA CCTCATAACA GTGTTGGTGA TGGGTCCAGA TGGGAATCTC 
TTTGCTGCTG AGGTCAGTGA TCAGCTAAAT GGTACCTACT TAGTGAGCTA CCGGCCTCAA 
CTGGAAGGGG AGCATCTTAT TTCAGTTATG GTTTGCAACC AACACATAGA AAACAGTCCC 
TTTAAAGTCA ATGTAAAATC TGGACGTTGC TACCTGGGTA TTGGACTTCC TACCCTCTCT 
TTTGGTGGGG AGGGTGATCA TGATGGAAAA CTTTGCCGCC CCTGGGGAGT TTGTGTTGAC 
AGGGAAGGTT ACATAATTGT GGCTGATAGG AGCAACAACC GTGTGCAAAT CTTTAAGCCA 
TGTGGAACCT TCCATCACAA ATTTGGCAGC CTTGGCTCTA GACCTGGACA GTTTGATCGA 
CCTGCTGGAG TAGCTTGCGA CAATTCACGC AGAATAGTCG TGGCAGACAA AGACAACCAC 
CGTGTTCAGA TATTTACATT TGAAGGGCAA TTCTTACTAA AGTTTGGTGA AAAGGGGACC 
AAGAATGGCC AATTCAACTA CCCTTGGGAT GTGGCAGTTA ATTCTGAGGG AAAGATCCTA 
GTTTCAGACA CCCGAAACCA CCGTGTCCAG CTGTTTGGTC CTGATGGGAC ATTCCTTAAC 
AAGTATGGCT TCGAGGGTGC ACTCTGGAAG CACTTTGACT CTCCCCGAGG TGTAGCATTT 
AGCCAGGATG GCTATTTGGT AGTGACTGAC TTCAATAATC ATCGGTTATT AATAATCAAG 
CCAGATTGTC AGTCGGCACA TTTCTTGGGC ACTGAGGGCA CAGGCAATGG GCAGTTTTTG 
CGTCCACAGG GTGTAGCAGT TGACCAGGAA GGGCGCATCA TTGTGGCAGA CTCCAGAAAT 
CACAGAGTCC AGATATTTGA GCCTAATGGC AGCTTCCTGT GCAAGTTTGG AACTCAAGGA 
AGTGGCTTTG GTCAAATGGA TCGCCCGTCT GGTATAGCTG TAACTCCCGA TGGCACAATT 
GTTGTGGTGG ATTTTGGAAA TAACCGCATT CTAGCCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915429.1
CDS	1225..3786
Translation	MASFPESDFQLCPLCKEMCVSSAPISSSSSSSSSSQVSTSSSSAGSAGGGTGLASAPPRRLHVLPCLHAFCRQCLEGRRSPGDSLQLRCPVCDHKVLISEAGMDALPSSTFLHLSNLLDAVVGAADEQQQSNGGRTASNRQRSASCSSSGLLRRAPPSQSEPRCSSCDDGNGASSHCLDCQENLCDNCLRAHQRVRLTKDHFIERFPASPCSSAASSAATTSSSSSSAFSLLPVYPERLYCQQHDEEIYKFGIRKRVYTLADLVRIKDWVLHFYCDSCSVPICRECTMGRHAGHSFVYLQEALQDSRALTIQLLADAQQGRQAIQLSLEQAQALAEQVEMKAKVVQSEIKAVTSRHKKALEERECELLWKVEKIRQVKAKSLYHQVEKLHQALNKLDSTINAVQQVLEEGCTMDILIARDRVLAQVQDVKNARGLLQLQEDDRIMFTPPDQALYLAIKSLGLVSSGAFAALTKATGEGLKRALQGKVASFTVIGYDHDGEARLSGGDLITVLVMGPDGNLFAAEVSDQLNGTYLVSYRPQLEGEHLISVMVCNQHIENSPFKVNVKSGRCYLGIGLPTLSFGGEGDHDGKLCRPWGVCVDREGYIIVADRSNNRVQIFKPCGTFHHKFGSLGSRPGQFDRPAGVACDNSRRIVVADKDNHRVQIFTFEGQFLLKFGEKGTKNGQFNYPWDVAVNSEGKILVSDTRNHRVQLFGPDGTFLNKYGFEGALWKHFDSPRGVAFSQDGYLVVTDFNNHRLLIIKPDCQSAHFLGTEGTGNGQFLRPQGVAVDQEGRIIVADSRNHRVQIFEPNGSFLCKFGTQGSGFGQMDRPSGIAVTPDGTIVVVDFGNNRILAF

Target ORF information:

RefSeq Version	XM_004915372.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915372.3

ORF Insert Sequence:

ATGGCTTCAT TCCCGGAGTC CGATTTCCAG CTGTGCCCCC TGTGTAAGGA GATGTGCGTG 
TCCTCTGCCC CCATCTCCTC CTCCAGCTCT TCCTCATCCT CCTCCCAGGT GTCCACCAGC 
AGCAGTAGCG CCGGCAGTGC AGGAGGAGGC ACCGGCTTGG CCTCGGCTCC CCCCCGCAGG 
CTGCACGTCT TGCCGTGCCT GCACGCCTTC TGTCGCCAGT GCCTGGAGGG GCGCCGGAGC 
CCCGGGGACT CTCTCCAGCT GCGCTGCCCG GTCTGTGACC ACAAGGTGCT CATCTCCGAG 
GCCGGCATGG ACGCGCTGCC CTCTTCTACC TTCTTGCACC TCAGCAACCT GCTGGACGCG 
GTGGTGGGCG CAGCCGACGA GCAGCAACAG AGTAACGGTG GCCGGACTGC TTCCAACAGA 
CAGCGTTCGG CTTCATGTTC TTCCTCCGGC CTCCTCCGCA GGGCGCCCCC CAGTCAGTCC 
GAACCGCGGT GCTCCTCATG CGACGATGGC AACGGGGCCA GCTCGCACTG TTTGGACTGC 
CAGGAGAACT TGTGCGACAA CTGCCTGAGG GCCCACCAGC GGGTGCGACT TACTAAGGAC 
CACTTCATAG AGCGCTTCCC CGCCTCCCCC TGCAGCTCCG CGGCTTCCAG TGCCGCCACT 
ACCTCGTCCT CTTCCAGCTC GGCCTTCTCC CTCCTGCCTG TGTACCCGGA GAGGCTCTAC 
TGCCAGCAGC ACGATGAGGA GATTTACAAA TTTGGAATAC GCAAGAGAGT GTATACCCTG 
GCGGATCTGG TGAGGATTAA GGATTGGGTC CTGCATTTCT ACTGTGACTC CTGCTCTGTT 
CCTATATGTC GTGAGTGCAC TATGGGCCGT CATGCTGGAC ACAGCTTTGT CTACCTCCAG 
GAAGCTCTTC AGGACTCAAG GGCACTAACT ATTCAGCTAT TAGCAGATGC CCAACAAGGC 
CGTCAAGCAA TTCAGCTGAG TCTAGAACAA GCTCAAGCAT TAGCAGAACA AGTTGAGATG 
AAAGCCAAAG TGGTGCAGTC TGAGATCAAA GCAGTCACAT CTAGACACAA GAAGGCATTG 
GAAGAGAGAG AGTGTGAGCT GCTCTGGAAG GTTGAAAAGA TACGTCAGGT GAAAGCAAAA 
TCACTTTATC ACCAAGTTGA AAAACTCCAC CAGGCCCTTA ATAAATTGGA CAGCACAATA 
AATGCAGTGC AGCAAGTCTT GGAGGAAGGT TGTACTATGG ATATCCTGAT AGCTCGTGAT 
CGTGTTTTGG CTCAGGTGCA GGATGTAAAG AATGCTAGAG GCCTTTTACA ACTTCAAGAA 
GATGACAGGA TTATGTTTAC TCCTCCTGAC CAAGCCTTAT ACCTTGCCAT CAAATCCTTG 
GGTCTTGTCA GCAGTGGAGC TTTTGCAGCT TTGACTAAAG CCACTGGAGA AGGACTAAAG 
CGTGCCCTAC AGGGAAAAGT TGCATCATTC ACTGTTATAG GATATGACCA TGATGGAGAA 
GCCCGCCTCT CAGGTGGGGA CCTCATAACA GTGTTGGTGA TGGGTCCAGA TGGGAATCTC 
TTTGCTGCTG AGGTCAGTGA TCAGCTAAAT GGTACCTACT TAGTGAGCTA CCGGCCTCAA 
CTGGAAGGGG AGCATCTTAT TTCAGTTATG GTTTGCAACC AACACATAGA AAACAGTCCC 
TTTAAAGTCA ATGTAAAATC TGGACGTTGC TACCTGGGTA TTGGACTTCC TACCCTCTCT 
TTTGGTGGGG AGGGTGATCA TGATGGAAAA CTTTGCCGCC CCTGGGGAGT TTGTGTTGAC 
AGGGAAGGTT ACATAATTGT GGCTGATAGG AGCAACAACC GTGTGCAAAT CTTTAAGCCA 
TGTGGAACCT TCCATCACAA ATTTGGCAGC CTTGGCTCTA GACCTGGACA GTTTGATCGA 
CCTGCTGGAG TAGCTTGCGA CAATTCACGC AGAATAGTCG TGGCAGACAA AGACAACCAC 
CGTGTTCAGA TATTTACATT TGAAGGGCAA TTCTTACTAA AGTTTGGTGA AAAGGGGACC 
AAGAATGGCC AATTCAACTA CCCTTGGGAT GTGGCAGTTA ATTCTGAGGG AAAGATCCTA 
GTTTCAGACA CCCGAAACCA CCGTGTCCAG CTGTTTGGTC CTGATGGGAC ATTCCTTAAC 
AAGTATGGCT TCGAGGGTGC ACTCTGGAAG CACTTTGACT CTCCCCGAGG TGTAGCATTT 
AGCCAGGATG GCTATTTGGT AGTGACTGAC TTCAATAATC ATCGGTTATT AATAATCAAG 
CCAGATTGTC AGTCGGCACA TTTCTTGGGC ACTGAGGGCA CAGGCAATGG GCAGTTTTTG 
CGTCCACAGG GTGTAGCAGT TGACCAGGAA GGGCGCATCA TTGTGGCAGA CTCCAGAAAT 
CACAGAGTCC AGATATTTGA GCCTAATGGC AGCTTCCTGT GCAAGTTTGG AACTCAAGGA 
AGTGGCTTTG GTCAAATGGA TCGCCCGTCT GGTATAGCTG TAACTCCCGA TGGCACAATT 
GTTGTGGTGG ATTTTGGAAA TAACCGCATT CTAGCCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim71 ( XM_004915373.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915430.1 Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa11891
Clone ID Related Accession (Same CDS sequence)	XM_004915373.3
Accession Version	XM_004915373.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase TRIM71 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915373.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCAT TCCCGGAGTC CGATTTCCAG CTGTGCCCCC TGTGTAAGGA GATGTGCGTG 
TCCTCTGCCC CCATCTCCTC CTCCAGCTCT TCCTCATCCT CCTCCCAGGT GTCCACCAGC 
AGCAGTAGCG CCGGCAGTGC AGGAGGAGGC ACCGGCTTGG CCTCGGCTCC CCCCCGCAGG 
CTGCACGTCT TGCCGTGCCT GCACGCCTTC TGTCGCCAGT GCCTGGAGGG GCGCCGGAGC 
CCCGGGGACT CTCTCCAGCT GCGCTGCCCG GTCTGTGACC ACAAGGTGCT CATCTCCGAG 
GCCGGCATGG ACGCGCTGCC CTCTTCTACC TTCTTGCACC TCAGCAACCT GCTGGACGCG 
GTGGTGGGCG CAGCCGACGA GCAGCAACAG AGTAACGGTG GCCGGACTGC TTCCAACAGA 
CAGCGTTCGG CTTCATGTTC TTCCTCCGGC CTCCTCCGCA GGGCGCCCCC CAGTCAGTCC 
GAACCGCGGT GCTCCTCATG CGACGATGGC AACGGGGCCA GCTCGCACTG TTTGGACTGC 
CAGGAGAACT TGTGCGACAA CTGCCTGAGG GCCCACCAGC GGGTGCGACT TACTAAGGAC 
CACTTCATAG AGCGCTTCCC CGCCTCCCCC TGCAGCTCCG CGGCTTCCAG TGCCGCCACT 
ACCTCGTCCT CTTCCAGCTC GGCCTTCTCC CTCCTGCCTG TGTACCCGGA GAGGCTCTAC 
TGCCAGCAGC ACGATGAGGA GATTTACAAA TTTGGAATAC GCAAGAGAGT GTATACCCTG 
GCGGATCTGG TCCTGCATTT CTACTGTGAC TCCTGCTCTG TTCCTATATG TCGTGAGTGC 
ACTATGGGCC GTCATGCTGG ACACAGCTTT GTCTACCTCC AGGAAGCTCT TCAGGACTCA 
AGGGCACTAA CTATTCAGCT ATTAGCAGAT GCCCAACAAG GCCGTCAAGC AATTCAGCTG 
AGTCTAGAAC AAGCTCAAGC ATTAGCAGAA CAAGTTGAGA TGAAAGCCAA AGTGGTGCAG 
TCTGAGATCA AAGCAGTCAC ATCTAGACAC AAGAAGGCAT TGGAAGAGAG AGAGTGTGAG 
CTGCTCTGGA AGGTTGAAAA GATACGTCAG GTGAAAGCAA AATCACTTTA TCACCAAGTT 
GAAAAACTCC ACCAGGCCCT TAATAAATTG GACAGCACAA TAAATGCAGT GCAGCAAGTC 
TTGGAGGAAG GTTGTACTAT GGATATCCTG ATAGCTCGTG ATCGTGTTTT GGCTCAGGTG 
CAGGATGTAA AGAATGCTAG AGGCCTTTTA CAACTTCAAG AAGATGACAG GATTATGTTT 
ACTCCTCCTG ACCAAGCCTT ATACCTTGCC ATCAAATCCT TGGGTCTTGT CAGCAGTGGA 
GCTTTTGCAG CTTTGACTAA AGCCACTGGA GAAGGACTAA AGCGTGCCCT ACAGGGAAAA 
GTTGCATCAT TCACTGTTAT AGGATATGAC CATGATGGAG AAGCCCGCCT CTCAGGTGGG 
GACCTCATAA CAGTGTTGGT GATGGGTCCA GATGGGAATC TCTTTGCTGC TGAGGTCAGT 
GATCAGCTAA ATGGTACCTA CTTAGTGAGC TACCGGCCTC AACTGGAAGG GGAGCATCTT 
ATTTCAGTTA TGGTTTGCAA CCAACACATA GAAAACAGTC CCTTTAAAGT CAATGTAAAA 
TCTGGACGTT GCTACCTGGG TATTGGACTT CCTACCCTCT CTTTTGGTGG GGAGGGTGAT 
CATGATGGAA AACTTTGCCG CCCCTGGGGA GTTTGTGTTG ACAGGGAAGG TTACATAATT 
GTGGCTGATA GGAGCAACAA CCGTGTGCAA ATCTTTAAGC CATGTGGAAC CTTCCATCAC 
AAATTTGGCA GCCTTGGCTC TAGACCTGGA CAGTTTGATC GACCTGCTGG AGTAGCTTGC 
GACAATTCAC GCAGAATAGT CGTGGCAGAC AAAGACAACC ACCGTGTTCA GATATTTACA 
TTTGAAGGGC AATTCTTACT AAAGTTTGGT GAAAAGGGGA CCAAGAATGG CCAATTCAAC 
TACCCTTGGG ATGTGGCAGT TAATTCTGAG GGAAAGATCC TAGTTTCAGA CACCCGAAAC 
CACCGTGTCC AGCTGTTTGG TCCTGATGGG ACATTCCTTA ACAAGTATGG CTTCGAGGGT 
GCACTCTGGA AGCACTTTGA CTCTCCCCGA GGTGTAGCAT TTAGCCAGGA TGGCTATTTG 
GTAGTGACTG ACTTCAATAA TCATCGGTTA TTAATAATCA AGCCAGATTG TCAGTCGGCA 
CATTTCTTGG GCACTGAGGG CACAGGCAAT GGGCAGTTTT TGCGTCCACA GGGTGTAGCA 
GTTGACCAGG AAGGGCGCAT CATTGTGGCA GACTCCAGAA ATCACAGAGT CCAGATATTT 
GAGCCTAATG GCAGCTTCCT GTGCAAGTTT GGAACTCAAG GAAGTGGCTT TGGTCAAATG 
GATCGCCCGT CTGGTATAGC TGTAACTCCC GATGGCACAA TTGTTGTGGT GGATTTTGGA 
AATAACCGCA TTCTAGCCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915430.1
CDS	1225..3768
Translation	MASFPESDFQLCPLCKEMCVSSAPISSSSSSSSSSQVSTSSSSAGSAGGGTGLASAPPRRLHVLPCLHAFCRQCLEGRRSPGDSLQLRCPVCDHKVLISEAGMDALPSSTFLHLSNLLDAVVGAADEQQQSNGGRTASNRQRSASCSSSGLLRRAPPSQSEPRCSSCDDGNGASSHCLDCQENLCDNCLRAHQRVRLTKDHFIERFPASPCSSAASSAATTSSSSSSAFSLLPVYPERLYCQQHDEEIYKFGIRKRVYTLADLVLHFYCDSCSVPICRECTMGRHAGHSFVYLQEALQDSRALTIQLLADAQQGRQAIQLSLEQAQALAEQVEMKAKVVQSEIKAVTSRHKKALEERECELLWKVEKIRQVKAKSLYHQVEKLHQALNKLDSTINAVQQVLEEGCTMDILIARDRVLAQVQDVKNARGLLQLQEDDRIMFTPPDQALYLAIKSLGLVSSGAFAALTKATGEGLKRALQGKVASFTVIGYDHDGEARLSGGDLITVLVMGPDGNLFAAEVSDQLNGTYLVSYRPQLEGEHLISVMVCNQHIENSPFKVNVKSGRCYLGIGLPTLSFGGEGDHDGKLCRPWGVCVDREGYIIVADRSNNRVQIFKPCGTFHHKFGSLGSRPGQFDRPAGVACDNSRRIVVADKDNHRVQIFTFEGQFLLKFGEKGTKNGQFNYPWDVAVNSEGKILVSDTRNHRVQLFGPDGTFLNKYGFEGALWKHFDSPRGVAFSQDGYLVVTDFNNHRLLIIKPDCQSAHFLGTEGTGNGQFLRPQGVAVDQEGRIIVADSRNHRVQIFEPNGSFLCKFGTQGSGFGQMDRPSGIAVTPDGTIVVVDFGNNRILAF

Target ORF information:

RefSeq Version	XM_004915373.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915373.3

ORF Insert Sequence:

ATGGCTTCAT TCCCGGAGTC CGATTTCCAG CTGTGCCCCC TGTGTAAGGA GATGTGCGTG 
TCCTCTGCCC CCATCTCCTC CTCCAGCTCT TCCTCATCCT CCTCCCAGGT GTCCACCAGC 
AGCAGTAGCG CCGGCAGTGC AGGAGGAGGC ACCGGCTTGG CCTCGGCTCC CCCCCGCAGG 
CTGCACGTCT TGCCGTGCCT GCACGCCTTC TGTCGCCAGT GCCTGGAGGG GCGCCGGAGC 
CCCGGGGACT CTCTCCAGCT GCGCTGCCCG GTCTGTGACC ACAAGGTGCT CATCTCCGAG 
GCCGGCATGG ACGCGCTGCC CTCTTCTACC TTCTTGCACC TCAGCAACCT GCTGGACGCG 
GTGGTGGGCG CAGCCGACGA GCAGCAACAG AGTAACGGTG GCCGGACTGC TTCCAACAGA 
CAGCGTTCGG CTTCATGTTC TTCCTCCGGC CTCCTCCGCA GGGCGCCCCC CAGTCAGTCC 
GAACCGCGGT GCTCCTCATG CGACGATGGC AACGGGGCCA GCTCGCACTG TTTGGACTGC 
CAGGAGAACT TGTGCGACAA CTGCCTGAGG GCCCACCAGC GGGTGCGACT TACTAAGGAC 
CACTTCATAG AGCGCTTCCC CGCCTCCCCC TGCAGCTCCG CGGCTTCCAG TGCCGCCACT 
ACCTCGTCCT CTTCCAGCTC GGCCTTCTCC CTCCTGCCTG TGTACCCGGA GAGGCTCTAC 
TGCCAGCAGC ACGATGAGGA GATTTACAAA TTTGGAATAC GCAAGAGAGT GTATACCCTG 
GCGGATCTGG TCCTGCATTT CTACTGTGAC TCCTGCTCTG TTCCTATATG TCGTGAGTGC 
ACTATGGGCC GTCATGCTGG ACACAGCTTT GTCTACCTCC AGGAAGCTCT TCAGGACTCA 
AGGGCACTAA CTATTCAGCT ATTAGCAGAT GCCCAACAAG GCCGTCAAGC AATTCAGCTG 
AGTCTAGAAC AAGCTCAAGC ATTAGCAGAA CAAGTTGAGA TGAAAGCCAA AGTGGTGCAG 
TCTGAGATCA AAGCAGTCAC ATCTAGACAC AAGAAGGCAT TGGAAGAGAG AGAGTGTGAG 
CTGCTCTGGA AGGTTGAAAA GATACGTCAG GTGAAAGCAA AATCACTTTA TCACCAAGTT 
GAAAAACTCC ACCAGGCCCT TAATAAATTG GACAGCACAA TAAATGCAGT GCAGCAAGTC 
TTGGAGGAAG GTTGTACTAT GGATATCCTG ATAGCTCGTG ATCGTGTTTT GGCTCAGGTG 
CAGGATGTAA AGAATGCTAG AGGCCTTTTA CAACTTCAAG AAGATGACAG GATTATGTTT 
ACTCCTCCTG ACCAAGCCTT ATACCTTGCC ATCAAATCCT TGGGTCTTGT CAGCAGTGGA 
GCTTTTGCAG CTTTGACTAA AGCCACTGGA GAAGGACTAA AGCGTGCCCT ACAGGGAAAA 
GTTGCATCAT TCACTGTTAT AGGATATGAC CATGATGGAG AAGCCCGCCT CTCAGGTGGG 
GACCTCATAA CAGTGTTGGT GATGGGTCCA GATGGGAATC TCTTTGCTGC TGAGGTCAGT 
GATCAGCTAA ATGGTACCTA CTTAGTGAGC TACCGGCCTC AACTGGAAGG GGAGCATCTT 
ATTTCAGTTA TGGTTTGCAA CCAACACATA GAAAACAGTC CCTTTAAAGT CAATGTAAAA 
TCTGGACGTT GCTACCTGGG TATTGGACTT CCTACCCTCT CTTTTGGTGG GGAGGGTGAT 
CATGATGGAA AACTTTGCCG CCCCTGGGGA GTTTGTGTTG ACAGGGAAGG TTACATAATT 
GTGGCTGATA GGAGCAACAA CCGTGTGCAA ATCTTTAAGC CATGTGGAAC CTTCCATCAC 
AAATTTGGCA GCCTTGGCTC TAGACCTGGA CAGTTTGATC GACCTGCTGG AGTAGCTTGC 
GACAATTCAC GCAGAATAGT CGTGGCAGAC AAAGACAACC ACCGTGTTCA GATATTTACA 
TTTGAAGGGC AATTCTTACT AAAGTTTGGT GAAAAGGGGA CCAAGAATGG CCAATTCAAC 
TACCCTTGGG ATGTGGCAGT TAATTCTGAG GGAAAGATCC TAGTTTCAGA CACCCGAAAC 
CACCGTGTCC AGCTGTTTGG TCCTGATGGG ACATTCCTTA ACAAGTATGG CTTCGAGGGT 
GCACTCTGGA AGCACTTTGA CTCTCCCCGA GGTGTAGCAT TTAGCCAGGA TGGCTATTTG 
GTAGTGACTG ACTTCAATAA TCATCGGTTA TTAATAATCA AGCCAGATTG TCAGTCGGCA 
CATTTCTTGG GCACTGAGGG CACAGGCAAT GGGCAGTTTT TGCGTCCACA GGGTGTAGCA 
GTTGACCAGG AAGGGCGCAT CATTGTGGCA GACTCCAGAA ATCACAGAGT CCAGATATTT 
GAGCCTAATG GCAGCTTCCT GTGCAAGTTT GGAACTCAAG GAAGTGGCTT TGGTCAAATG 
GATCGCCCGT CTGGTATAGC TGTAACTCCC GATGGCACAA TTGTTGTGGT GGATTTTGGA 
AATAACCGCA TTCTAGCCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim71 ( XM_002937846.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937892.1 Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X3, mRNA.

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CloneID	OXa11892
Clone ID Related Accession (Same CDS sequence)	XM_002937846.4
Accession Version	XM_002937846.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase TRIM71 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002937846.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCAT TCCCGGAGTC CGATTTCCAG CTGTGCCCCC TGTGTAAGGA GATGTGCGTG 
TCCTCTGCCC CCATCTCCTC CTCCAGCTCT TCCTCATCCT CCTCCCAGGT GTCCACCAGC 
AGCAGTAGCG CCGGCAGTGC AGGAGGAGGC ACCGGCTTGG CCTCGGCTCC CCCCCGCAGG 
CTGCACGTCT TGCCGTGCCT GCACGCCTTC TGTCGCCAGT GCCTGGAGGG GCGCCGGAGC 
CCCGGGGACT CTCTCCAGCT GCGCTGCCCG GTCTGTGACC ACAAGGTGCT CATCTCCGAG 
GCCGGCATGG ACGCGCTGCC CTCTTCTACC TTCTTGCACC TCAGCAACCT GCTGGACGCG 
GTGGTGGGCG CAGCCGACGA GCAGCAACAG AGTAACGGTG GCCGGACTGC TTCCAACAGA 
CAGCGTTCGG CTTCATGTTC TTCCTCCGGC CTCCTCCGCA GGGCGCCCCC CAGTCAGTCC 
GAACCGCGGT GCTCCTCATG CGACGATGGC AACGGGGCCA GCTCGCACTG TTTGGACTGC 
CAGGAGAACT TGTGCGACAA CTGCCTGAGG GCCCACCAGC GGGTGCGACT TACTAAGGAC 
CACTTCATAG AGCGCTTCCC CGCCTCCCCC TGCAGCTCCG CGGCTTCCAG TGCCGCCACT 
ACCTCGTCCT CTTCCAGCTC GGCCTTCTCC CTCCTGCCTG TGTACCCGGA GAGGCTCTAC 
TGCCAGCAGC ACGATGAGGA GGTCCTGCAT TTCTACTGTG ACTCCTGCTC TGTTCCTATA 
TGTCGTGAGT GCACTATGGG CCGTCATGCT GGACACAGCT TTGTCTACCT CCAGGAAGCT 
CTTCAGGACT CAAGGGCACT AACTATTCAG CTATTAGCAG ATGCCCAACA AGGCCGTCAA 
GCAATTCAGC TGAGTCTAGA ACAAGCTCAA GCATTAGCAG AACAAGTTGA GATGAAAGCC 
AAAGTGGTGC AGTCTGAGAT CAAAGCAGTC ACATCTAGAC ACAAGAAGGC ATTGGAAGAG 
AGAGAGTGTG AGCTGCTCTG GAAGGTTGAA AAGATACGTC AGGTGAAAGC AAAATCACTT 
TATCACCAAG TTGAAAAACT CCACCAGGCC CTTAATAAAT TGGACAGCAC AATAAATGCA 
GTGCAGCAAG TCTTGGAGGA AGGTTGTACT ATGGATATCC TGATAGCTCG TGATCGTGTT 
TTGGCTCAGG TGCAGGATGT AAAGAATGCT AGAGGCCTTT TACAACTTCA AGAAGATGAC 
AGGATTATGT TTACTCCTCC TGACCAAGCC TTATACCTTG CCATCAAATC CTTGGGTCTT 
GTCAGCAGTG GAGCTTTTGC AGCTTTGACT AAAGCCACTG GAGAAGGACT AAAGCGTGCC 
CTACAGGGAA AAGTTGCATC ATTCACTGTT ATAGGATATG ACCATGATGG AGAAGCCCGC 
CTCTCAGGTG GGGACCTCAT AACAGTGTTG GTGATGGGTC CAGATGGGAA TCTCTTTGCT 
GCTGAGGTCA GTGATCAGCT AAATGGTACC TACTTAGTGA GCTACCGGCC TCAACTGGAA 
GGGGAGCATC TTATTTCAGT TATGGTTTGC AACCAACACA TAGAAAACAG TCCCTTTAAA 
GTCAATGTAA AATCTGGACG TTGCTACCTG GGTATTGGAC TTCCTACCCT CTCTTTTGGT 
GGGGAGGGTG ATCATGATGG AAAACTTTGC CGCCCCTGGG GAGTTTGTGT TGACAGGGAA 
GGTTACATAA TTGTGGCTGA TAGGAGCAAC AACCGTGTGC AAATCTTTAA GCCATGTGGA 
ACCTTCCATC ACAAATTTGG CAGCCTTGGC TCTAGACCTG GACAGTTTGA TCGACCTGCT 
GGAGTAGCTT GCGACAATTC ACGCAGAATA GTCGTGGCAG ACAAAGACAA CCACCGTGTT 
CAGATATTTA CATTTGAAGG GCAATTCTTA CTAAAGTTTG GTGAAAAGGG GACCAAGAAT 
GGCCAATTCA ACTACCCTTG GGATGTGGCA GTTAATTCTG AGGGAAAGAT CCTAGTTTCA 
GACACCCGAA ACCACCGTGT CCAGCTGTTT GGTCCTGATG GGACATTCCT TAACAAGTAT 
GGCTTCGAGG GTGCACTCTG GAAGCACTTT GACTCTCCCC GAGGTGTAGC ATTTAGCCAG 
GATGGCTATT TGGTAGTGAC TGACTTCAAT AATCATCGGT TATTAATAAT CAAGCCAGAT 
TGTCAGTCGG CACATTTCTT GGGCACTGAG GGCACAGGCA ATGGGCAGTT TTTGCGTCCA 
CAGGGTGTAG CAGTTGACCA GGAAGGGCGC ATCATTGTGG CAGACTCCAG AAATCACAGA 
GTCCAGATAT TTGAGCCTAA TGGCAGCTTC CTGTGCAAGT TTGGAACTCA AGGAAGTGGC 
TTTGGTCAAA TGGATCGCCC GTCTGGTATA GCTGTAACTC CCGATGGCAC AATTGTTGTG 
GTGGATTTTG GAAATAACCG CATTCTAGCC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937892.1
CDS	1224..3719
Translation	MASFPESDFQLCPLCKEMCVSSAPISSSSSSSSSSQVSTSSSSAGSAGGGTGLASAPPRRLHVLPCLHAFCRQCLEGRRSPGDSLQLRCPVCDHKVLISEAGMDALPSSTFLHLSNLLDAVVGAADEQQQSNGGRTASNRQRSASCSSSGLLRRAPPSQSEPRCSSCDDGNGASSHCLDCQENLCDNCLRAHQRVRLTKDHFIERFPASPCSSAASSAATTSSSSSSAFSLLPVYPERLYCQQHDEEVLHFYCDSCSVPICRECTMGRHAGHSFVYLQEALQDSRALTIQLLADAQQGRQAIQLSLEQAQALAEQVEMKAKVVQSEIKAVTSRHKKALEERECELLWKVEKIRQVKAKSLYHQVEKLHQALNKLDSTINAVQQVLEEGCTMDILIARDRVLAQVQDVKNARGLLQLQEDDRIMFTPPDQALYLAIKSLGLVSSGAFAALTKATGEGLKRALQGKVASFTVIGYDHDGEARLSGGDLITVLVMGPDGNLFAAEVSDQLNGTYLVSYRPQLEGEHLISVMVCNQHIENSPFKVNVKSGRCYLGIGLPTLSFGGEGDHDGKLCRPWGVCVDREGYIIVADRSNNRVQIFKPCGTFHHKFGSLGSRPGQFDRPAGVACDNSRRIVVADKDNHRVQIFTFEGQFLLKFGEKGTKNGQFNYPWDVAVNSEGKILVSDTRNHRVQLFGPDGTFLNKYGFEGALWKHFDSPRGVAFSQDGYLVVTDFNNHRLLIIKPDCQSAHFLGTEGTGNGQFLRPQGVAVDQEGRIIVADSRNHRVQIFEPNGSFLCKFGTQGSGFGQMDRPSGIAVTPDGTIVVVDFGNNRILAF

Target ORF information:

RefSeq Version	XM_002937846.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937846.4

ORF Insert Sequence:

ATGGCTTCAT TCCCGGAGTC CGATTTCCAG CTGTGCCCCC TGTGTAAGGA GATGTGCGTG 
TCCTCTGCCC CCATCTCCTC CTCCAGCTCT TCCTCATCCT CCTCCCAGGT GTCCACCAGC 
AGCAGTAGCG CCGGCAGTGC AGGAGGAGGC ACCGGCTTGG CCTCGGCTCC CCCCCGCAGG 
CTGCACGTCT TGCCGTGCCT GCACGCCTTC TGTCGCCAGT GCCTGGAGGG GCGCCGGAGC 
CCCGGGGACT CTCTCCAGCT GCGCTGCCCG GTCTGTGACC ACAAGGTGCT CATCTCCGAG 
GCCGGCATGG ACGCGCTGCC CTCTTCTACC TTCTTGCACC TCAGCAACCT GCTGGACGCG 
GTGGTGGGCG CAGCCGACGA GCAGCAACAG AGTAACGGTG GCCGGACTGC TTCCAACAGA 
CAGCGTTCGG CTTCATGTTC TTCCTCCGGC CTCCTCCGCA GGGCGCCCCC CAGTCAGTCC 
GAACCGCGGT GCTCCTCATG CGACGATGGC AACGGGGCCA GCTCGCACTG TTTGGACTGC 
CAGGAGAACT TGTGCGACAA CTGCCTGAGG GCCCACCAGC GGGTGCGACT TACTAAGGAC 
CACTTCATAG AGCGCTTCCC CGCCTCCCCC TGCAGCTCCG CGGCTTCCAG TGCCGCCACT 
ACCTCGTCCT CTTCCAGCTC GGCCTTCTCC CTCCTGCCTG TGTACCCGGA GAGGCTCTAC 
TGCCAGCAGC ACGATGAGGA GGTCCTGCAT TTCTACTGTG ACTCCTGCTC TGTTCCTATA 
TGTCGTGAGT GCACTATGGG CCGTCATGCT GGACACAGCT TTGTCTACCT CCAGGAAGCT 
CTTCAGGACT CAAGGGCACT AACTATTCAG CTATTAGCAG ATGCCCAACA AGGCCGTCAA 
GCAATTCAGC TGAGTCTAGA ACAAGCTCAA GCATTAGCAG AACAAGTTGA GATGAAAGCC 
AAAGTGGTGC AGTCTGAGAT CAAAGCAGTC ACATCTAGAC ACAAGAAGGC ATTGGAAGAG 
AGAGAGTGTG AGCTGCTCTG GAAGGTTGAA AAGATACGTC AGGTGAAAGC AAAATCACTT 
TATCACCAAG TTGAAAAACT CCACCAGGCC CTTAATAAAT TGGACAGCAC AATAAATGCA 
GTGCAGCAAG TCTTGGAGGA AGGTTGTACT ATGGATATCC TGATAGCTCG TGATCGTGTT 
TTGGCTCAGG TGCAGGATGT AAAGAATGCT AGAGGCCTTT TACAACTTCA AGAAGATGAC 
AGGATTATGT TTACTCCTCC TGACCAAGCC TTATACCTTG CCATCAAATC CTTGGGTCTT 
GTCAGCAGTG GAGCTTTTGC AGCTTTGACT AAAGCCACTG GAGAAGGACT AAAGCGTGCC 
CTACAGGGAA AAGTTGCATC ATTCACTGTT ATAGGATATG ACCATGATGG AGAAGCCCGC 
CTCTCAGGTG GGGACCTCAT AACAGTGTTG GTGATGGGTC CAGATGGGAA TCTCTTTGCT 
GCTGAGGTCA GTGATCAGCT AAATGGTACC TACTTAGTGA GCTACCGGCC TCAACTGGAA 
GGGGAGCATC TTATTTCAGT TATGGTTTGC AACCAACACA TAGAAAACAG TCCCTTTAAA 
GTCAATGTAA AATCTGGACG TTGCTACCTG GGTATTGGAC TTCCTACCCT CTCTTTTGGT 
GGGGAGGGTG ATCATGATGG AAAACTTTGC CGCCCCTGGG GAGTTTGTGT TGACAGGGAA 
GGTTACATAA TTGTGGCTGA TAGGAGCAAC AACCGTGTGC AAATCTTTAA GCCATGTGGA 
ACCTTCCATC ACAAATTTGG CAGCCTTGGC TCTAGACCTG GACAGTTTGA TCGACCTGCT 
GGAGTAGCTT GCGACAATTC ACGCAGAATA GTCGTGGCAG ACAAAGACAA CCACCGTGTT 
CAGATATTTA CATTTGAAGG GCAATTCTTA CTAAAGTTTG GTGAAAAGGG GACCAAGAAT 
GGCCAATTCA ACTACCCTTG GGATGTGGCA GTTAATTCTG AGGGAAAGAT CCTAGTTTCA 
GACACCCGAA ACCACCGTGT CCAGCTGTTT GGTCCTGATG GGACATTCCT TAACAAGTAT 
GGCTTCGAGG GTGCACTCTG GAAGCACTTT GACTCTCCCC GAGGTGTAGC ATTTAGCCAG 
GATGGCTATT TGGTAGTGAC TGACTTCAAT AATCATCGGT TATTAATAAT CAAGCCAGAT 
TGTCAGTCGG CACATTTCTT GGGCACTGAG GGCACAGGCA ATGGGCAGTT TTTGCGTCCA 
CAGGGTGTAG CAGTTGACCA GGAAGGGCGC ATCATTGTGG CAGACTCCAG AAATCACAGA 
GTCCAGATAT TTGAGCCTAA TGGCAGCTTC CTGTGCAAGT TTGGAACTCA AGGAAGTGGC 
TTTGGTCAAA TGGATCGCCC GTCTGGTATA GCTGTAACTC CCGATGGCAC AATTGTTGTG 
GTGGATTTTG GAAATAACCG CATTCTAGCC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim71 ( XM_004915372.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915429.1 Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa60085
Clone ID Related Accession (Same CDS sequence)	XM_004915372.4
Accession Version	XM_004915372.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase TRIM71 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915372.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCAT TCCCGGAGTC CGATTTCCAG CTGTGCCCCC TGTGTAAGGA GATGTGCGTG 
TCCTCTGCCC CCATCTCCTC CTCCAGCTCT TCCTCATCCT CCTCCCAGGT GTCCACCAGC 
AGCAGTAGCG CCGGCAGTGC AGGAGGAGGC ACCGGCTTGG CCTCGGCTCC CCCCCGCAGG 
CTGCACGTCT TGCCGTGCCT GCACGCCTTC TGTCGCCAGT GCCTGGAGGG GCGCCGGAGC 
CCCGGGGACT CTCTCCAGCT GCGCTGCCCG GTCTGTGACC ACAAGGTGCT CATCTCCGAG 
GCCGGCATGG ACGCGCTGCC CTCTTCTACC TTCTTGCACC TCAGCAACCT GCTGGACGCG 
GTGGTGGGCG CAGCCGACGA GCAGCAACAG AGTAACGGTG GCCGGACTGC TTCCAACAGA 
CAGCGTTCGG CTTCATGTTC TTCCTCCGGC CTCCTCCGCA GGGCGCCCCC CAGTCAGTCC 
GAACCGCGGT GCTCCTCATG CGACGATGGC AACGGGGCCA GCTCGCACTG CTTGGACTGC 
CAGGAGAACT TGTGCGACAA CTGCCTGAGG GCCCACCAGC GGGTCCGACT TACTAAGGAC 
CACTTCATAG AGCGCTTCCC CGCCTCCCCC TGCAGCTCCG CGGCTTCCAG TGCCGCCACT 
ACCTCGTCCT CTTCCAGCTC GGCCTTCTCC CTCCTGCCTG TGTACCCGGA GAGGCTCTAC 
TGCCAGCAGC ACGATGAGGA GATTTACAAA TTTGGAATAC GCAAGAGAGT GTATACCCTG 
GCGGATCTGG TGAGGATTAA GGATTGGGTC CTGCATTTCT ACTGTGACTC CTGCTCTGTT 
CCTATATGTC GTGAGTGCAC TATGGGCCGT CATGCTGGAC ACAGCTTTGT CTACCTCCAG 
GAAGCTCTTC AGGACTCAAG GGCACTAACT ATTCAGCTAT TAGCAGATGC CCAACAAGGC 
CGTCAAGCAA TTCAGCTGAG TCTAGAACAA GCTCAAGCAT TAGCAGAACA AGTTGAGATG 
AAAGCCAAAG TGGTGCAGTC TGAGATCAAA GCAGTCACAT CTAGACACAA GAAGGCATTG 
GAAGAGAGAG AGTGTGAGCT GCTCTGGAAG GTTGAAAAGA TACGTCAGGT GAAAGCAAAA 
TCACTTTATC ACCAAGTTGA AAAACTCCAC CAGGCCCTTA ATAAGTTGGA CAGCACAATA 
AATGCAGTGC AGCAAGTCTT GGAGGAAGGT TGTACTATGG ATATCCTAAT AGCTCGTGAT 
CGTGTTTTGG CTCAGGTGCA GGATGTAAAG AATGCTAGAG GCCTTTTACA ACTTCAAGAA 
GATGACAGGA TTATGTTTAC TCCTCCTGAC CAAGCCTTAT ACCTTGCCAT CAAATCCTTG 
GGTCTTGTCA GCAGTGGAGC TTTTGCAGCT TTGACTAAAG CCACTGGAGA AGGACTAAAG 
CGTGCCCTAC AGGGAAAAGT TGCATCATTC ACTGTTATAG GATATGACCA TGATGGAGAA 
GCCCGCCTCT CAGGTGGGGA CCTCATAACA GTGTTGGTGA TGGGTCCAGA TGGGAATCTC 
TTTGCTGCTG AGGTCAGTGA TCAGCTAAAT GGTACCTACT TAGTGAGCTA CCGGCCTCAA 
CTGGAAGGGG AGCATCTTAT TTCAGTTATG GTTTGCAACC AACACATAGA AAACAGTCCC 
TTTAAAGTCA ATGTAAAATC TGGACGTTGC TACCTGGGTA TTGGACTTCC TACCCTCTCT 
TTTGGTGGGG AGGGTGATCA TGATGGAAAA CTTTGCCGCC CCTGGGGAGT TTGTGTTGAC 
AGGGAAGGTT ACATAATTGT GGCTGATAGG AGCAACAACC GTGTGCAAAT CTTTAAGCCA 
TGTGGAACCT TCCATCACAA ATTTGGCAGC CTTGGCTCTA GACCTGGACA GTTTGATCGA 
CCTGCTGGAG TAGCTTGCGA CAATTCACGC AGAATAGTCG TGGCAGACAA AGACAACCAC 
CGTGTTCAGA TATTTACATT TGAAGGGCAA TTCTTACTAA AGTTTGGTGA AAAGGGGACC 
AAGAATGGCC AATTCAACTA CCCTTGGGAT GTGGCAGTTA ATTCTGAGGG AAAGATCCTA 
GTTTCAGACA CCCGAAACCA CCGTGTCCAG CTGTTTGGTC CTGATGGGAC ATTCCTTAAC 
AAGTATGGCT TCGAGGGTGC ACTCTGGAAG CACTTTGACT CTCCCCGAGG TGTAGCATTT 
AGCCAGGATG GCTATTTGGT AGTGACTGAC TTCAATAATC ATCGGTTATT AATAATCAAG 
CCAGATTGTC AGTCGGCACA TTTCTTGGGC ACTGAGGGCA CAGGCAATGG GCAGTTTTTG 
CGTCCACAGG GTGTAGCAGT TGACCAGGAA GGGCGCATCA TTGTGGCAGA CTCCAGAAAT 
CACAGAGTCC AGATATTTGA GCCTAATGGC AGCTTCCTGT GCAAGTTTGG AACTCAAGGA 
AGTGGCTTTG GTCAAATGGA TCGCCCGTCT GGTATAGCTG TAACTCCCGA TGGCACAATT 
GTTGTGGTGG ATTTTGGAAA TAACCGCATT CTAGCCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915429.1
CDS	909..3470
Translation	MASFPESDFQLCPLCKEMCVSSAPISSSSSSSSSSQVSTSSSSAGSAGGGTGLASAPPRRLHVLPCLHAFCRQCLEGRRSPGDSLQLRCPVCDHKVLISEAGMDALPSSTFLHLSNLLDAVVGAADEQQQSNGGRTASNRQRSASCSSSGLLRRAPPSQSEPRCSSCDDGNGASSHCLDCQENLCDNCLRAHQRVRLTKDHFIERFPASPCSSAASSAATTSSSSSSAFSLLPVYPERLYCQQHDEEIYKFGIRKRVYTLADLVRIKDWVLHFYCDSCSVPICRECTMGRHAGHSFVYLQEALQDSRALTIQLLADAQQGRQAIQLSLEQAQALAEQVEMKAKVVQSEIKAVTSRHKKALEERECELLWKVEKIRQVKAKSLYHQVEKLHQALNKLDSTINAVQQVLEEGCTMDILIARDRVLAQVQDVKNARGLLQLQEDDRIMFTPPDQALYLAIKSLGLVSSGAFAALTKATGEGLKRALQGKVASFTVIGYDHDGEARLSGGDLITVLVMGPDGNLFAAEVSDQLNGTYLVSYRPQLEGEHLISVMVCNQHIENSPFKVNVKSGRCYLGIGLPTLSFGGEGDHDGKLCRPWGVCVDREGYIIVADRSNNRVQIFKPCGTFHHKFGSLGSRPGQFDRPAGVACDNSRRIVVADKDNHRVQIFTFEGQFLLKFGEKGTKNGQFNYPWDVAVNSEGKILVSDTRNHRVQLFGPDGTFLNKYGFEGALWKHFDSPRGVAFSQDGYLVVTDFNNHRLLIIKPDCQSAHFLGTEGTGNGQFLRPQGVAVDQEGRIIVADSRNHRVQIFEPNGSFLCKFGTQGSGFGQMDRPSGIAVTPDGTIVVVDFGNNRILAF

Target ORF information:

RefSeq Version	XM_004915372.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915372.4

ORF Insert Sequence:

ATGGCTTCAT TCCCGGAGTC CGATTTCCAG CTGTGCCCCC TGTGTAAGGA GATGTGCGTG 
TCCTCTGCCC CCATCTCCTC CTCCAGCTCT TCCTCATCCT CCTCCCAGGT GTCCACCAGC 
AGCAGTAGCG CCGGCAGTGC AGGAGGAGGC ACCGGCTTGG CCTCGGCTCC CCCCCGCAGG 
CTGCACGTCT TGCCGTGCCT GCACGCCTTC TGTCGCCAGT GCCTGGAGGG GCGCCGGAGC 
CCCGGGGACT CTCTCCAGCT GCGCTGCCCG GTCTGTGACC ACAAGGTGCT CATCTCCGAG 
GCCGGCATGG ACGCGCTGCC CTCTTCTACC TTCTTGCACC TCAGCAACCT GCTGGACGCG 
GTGGTGGGCG CAGCCGACGA GCAGCAACAG AGTAACGGTG GCCGGACTGC TTCCAACAGA 
CAGCGTTCGG CTTCATGTTC TTCCTCCGGC CTCCTCCGCA GGGCGCCCCC CAGTCAGTCC 
GAACCGCGGT GCTCCTCATG CGACGATGGC AACGGGGCCA GCTCGCACTG CTTGGACTGC 
CAGGAGAACT TGTGCGACAA CTGCCTGAGG GCCCACCAGC GGGTCCGACT TACTAAGGAC 
CACTTCATAG AGCGCTTCCC CGCCTCCCCC TGCAGCTCCG CGGCTTCCAG TGCCGCCACT 
ACCTCGTCCT CTTCCAGCTC GGCCTTCTCC CTCCTGCCTG TGTACCCGGA GAGGCTCTAC 
TGCCAGCAGC ACGATGAGGA GATTTACAAA TTTGGAATAC GCAAGAGAGT GTATACCCTG 
GCGGATCTGG TGAGGATTAA GGATTGGGTC CTGCATTTCT ACTGTGACTC CTGCTCTGTT 
CCTATATGTC GTGAGTGCAC TATGGGCCGT CATGCTGGAC ACAGCTTTGT CTACCTCCAG 
GAAGCTCTTC AGGACTCAAG GGCACTAACT ATTCAGCTAT TAGCAGATGC CCAACAAGGC 
CGTCAAGCAA TTCAGCTGAG TCTAGAACAA GCTCAAGCAT TAGCAGAACA AGTTGAGATG 
AAAGCCAAAG TGGTGCAGTC TGAGATCAAA GCAGTCACAT CTAGACACAA GAAGGCATTG 
GAAGAGAGAG AGTGTGAGCT GCTCTGGAAG GTTGAAAAGA TACGTCAGGT GAAAGCAAAA 
TCACTTTATC ACCAAGTTGA AAAACTCCAC CAGGCCCTTA ATAAGTTGGA CAGCACAATA 
AATGCAGTGC AGCAAGTCTT GGAGGAAGGT TGTACTATGG ATATCCTAAT AGCTCGTGAT 
CGTGTTTTGG CTCAGGTGCA GGATGTAAAG AATGCTAGAG GCCTTTTACA ACTTCAAGAA 
GATGACAGGA TTATGTTTAC TCCTCCTGAC CAAGCCTTAT ACCTTGCCAT CAAATCCTTG 
GGTCTTGTCA GCAGTGGAGC TTTTGCAGCT TTGACTAAAG CCACTGGAGA AGGACTAAAG 
CGTGCCCTAC AGGGAAAAGT TGCATCATTC ACTGTTATAG GATATGACCA TGATGGAGAA 
GCCCGCCTCT CAGGTGGGGA CCTCATAACA GTGTTGGTGA TGGGTCCAGA TGGGAATCTC 
TTTGCTGCTG AGGTCAGTGA TCAGCTAAAT GGTACCTACT TAGTGAGCTA CCGGCCTCAA 
CTGGAAGGGG AGCATCTTAT TTCAGTTATG GTTTGCAACC AACACATAGA AAACAGTCCC 
TTTAAAGTCA ATGTAAAATC TGGACGTTGC TACCTGGGTA TTGGACTTCC TACCCTCTCT 
TTTGGTGGGG AGGGTGATCA TGATGGAAAA CTTTGCCGCC CCTGGGGAGT TTGTGTTGAC 
AGGGAAGGTT ACATAATTGT GGCTGATAGG AGCAACAACC GTGTGCAAAT CTTTAAGCCA 
TGTGGAACCT TCCATCACAA ATTTGGCAGC CTTGGCTCTA GACCTGGACA GTTTGATCGA 
CCTGCTGGAG TAGCTTGCGA CAATTCACGC AGAATAGTCG TGGCAGACAA AGACAACCAC 
CGTGTTCAGA TATTTACATT TGAAGGGCAA TTCTTACTAA AGTTTGGTGA AAAGGGGACC 
AAGAATGGCC AATTCAACTA CCCTTGGGAT GTGGCAGTTA ATTCTGAGGG AAAGATCCTA 
GTTTCAGACA CCCGAAACCA CCGTGTCCAG CTGTTTGGTC CTGATGGGAC ATTCCTTAAC 
AAGTATGGCT TCGAGGGTGC ACTCTGGAAG CACTTTGACT CTCCCCGAGG TGTAGCATTT 
AGCCAGGATG GCTATTTGGT AGTGACTGAC TTCAATAATC ATCGGTTATT AATAATCAAG 
CCAGATTGTC AGTCGGCACA TTTCTTGGGC ACTGAGGGCA CAGGCAATGG GCAGTTTTTG 
CGTCCACAGG GTGTAGCAGT TGACCAGGAA GGGCGCATCA TTGTGGCAGA CTCCAGAAAT 
CACAGAGTCC AGATATTTGA GCCTAATGGC AGCTTCCTGT GCAAGTTTGG AACTCAAGGA 
AGTGGCTTTG GTCAAATGGA TCGCCCGTCT GGTATAGCTG TAACTCCCGA TGGCACAATT 
GTTGTGGTGG ATTTTGGAAA TAACCGCATT CTAGCCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim71 ( XM_004915373.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915430.1 Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X2, mRNA.

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CloneID	OXa60086
Clone ID Related Accession (Same CDS sequence)	XM_004915373.4
Accession Version	XM_004915373.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase TRIM71 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915373.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCAT TCCCGGAGTC CGATTTCCAG CTGTGCCCCC TGTGTAAGGA GATGTGCGTG 
TCCTCTGCCC CCATCTCCTC CTCCAGCTCT TCCTCATCCT CCTCCCAGGT GTCCACCAGC 
AGCAGTAGCG CCGGCAGTGC AGGAGGAGGC ACCGGCTTGG CCTCGGCTCC CCCCCGCAGG 
CTGCACGTCT TGCCGTGCCT GCACGCCTTC TGTCGCCAGT GCCTGGAGGG GCGCCGGAGC 
CCCGGGGACT CTCTCCAGCT GCGCTGCCCG GTCTGTGACC ACAAGGTGCT CATCTCCGAG 
GCCGGCATGG ACGCGCTGCC CTCTTCTACC TTCTTGCACC TCAGCAACCT GCTGGACGCG 
GTGGTGGGCG CAGCCGACGA GCAGCAACAG AGTAACGGTG GCCGGACTGC TTCCAACAGA 
CAGCGTTCGG CTTCATGTTC TTCCTCCGGC CTCCTCCGCA GGGCGCCCCC CAGTCAGTCC 
GAACCGCGGT GCTCCTCATG CGACGATGGC AACGGGGCCA GCTCGCACTG CTTGGACTGC 
CAGGAGAACT TGTGCGACAA CTGCCTGAGG GCCCACCAGC GGGTCCGACT TACTAAGGAC 
CACTTCATAG AGCGCTTCCC CGCCTCCCCC TGCAGCTCCG CGGCTTCCAG TGCCGCCACT 
ACCTCGTCCT CTTCCAGCTC GGCCTTCTCC CTCCTGCCTG TGTACCCGGA GAGGCTCTAC 
TGCCAGCAGC ACGATGAGGA GATTTACAAA TTTGGAATAC GCAAGAGAGT GTATACCCTG 
GCGGATCTGG TCCTGCATTT CTACTGTGAC TCCTGCTCTG TTCCTATATG TCGTGAGTGC 
ACTATGGGCC GTCATGCTGG ACACAGCTTT GTCTACCTCC AGGAAGCTCT TCAGGACTCA 
AGGGCACTAA CTATTCAGCT ATTAGCAGAT GCCCAACAAG GCCGTCAAGC AATTCAGCTG 
AGTCTAGAAC AAGCTCAAGC ATTAGCAGAA CAAGTTGAGA TGAAAGCCAA AGTGGTGCAG 
TCTGAGATCA AAGCAGTCAC ATCTAGACAC AAGAAGGCAT TGGAAGAGAG AGAGTGTGAG 
CTGCTCTGGA AGGTTGAAAA GATACGTCAG GTGAAAGCAA AATCACTTTA TCACCAAGTT 
GAAAAACTCC ACCAGGCCCT TAATAAGTTG GACAGCACAA TAAATGCAGT GCAGCAAGTC 
TTGGAGGAAG GTTGTACTAT GGATATCCTA ATAGCTCGTG ATCGTGTTTT GGCTCAGGTG 
CAGGATGTAA AGAATGCTAG AGGCCTTTTA CAACTTCAAG AAGATGACAG GATTATGTTT 
ACTCCTCCTG ACCAAGCCTT ATACCTTGCC ATCAAATCCT TGGGTCTTGT CAGCAGTGGA 
GCTTTTGCAG CTTTGACTAA AGCCACTGGA GAAGGACTAA AGCGTGCCCT ACAGGGAAAA 
GTTGCATCAT TCACTGTTAT AGGATATGAC CATGATGGAG AAGCCCGCCT CTCAGGTGGG 
GACCTCATAA CAGTGTTGGT GATGGGTCCA GATGGGAATC TCTTTGCTGC TGAGGTCAGT 
GATCAGCTAA ATGGTACCTA CTTAGTGAGC TACCGGCCTC AACTGGAAGG GGAGCATCTT 
ATTTCAGTTA TGGTTTGCAA CCAACACATA GAAAACAGTC CCTTTAAAGT CAATGTAAAA 
TCTGGACGTT GCTACCTGGG TATTGGACTT CCTACCCTCT CTTTTGGTGG GGAGGGTGAT 
CATGATGGAA AACTTTGCCG CCCCTGGGGA GTTTGTGTTG ACAGGGAAGG TTACATAATT 
GTGGCTGATA GGAGCAACAA CCGTGTGCAA ATCTTTAAGC CATGTGGAAC CTTCCATCAC 
AAATTTGGCA GCCTTGGCTC TAGACCTGGA CAGTTTGATC GACCTGCTGG AGTAGCTTGC 
GACAATTCAC GCAGAATAGT CGTGGCAGAC AAAGACAACC ACCGTGTTCA GATATTTACA 
TTTGAAGGGC AATTCTTACT AAAGTTTGGT GAAAAGGGGA CCAAGAATGG CCAATTCAAC 
TACCCTTGGG ATGTGGCAGT TAATTCTGAG GGAAAGATCC TAGTTTCAGA CACCCGAAAC 
CACCGTGTCC AGCTGTTTGG TCCTGATGGG ACATTCCTTA ACAAGTATGG CTTCGAGGGT 
GCACTCTGGA AGCACTTTGA CTCTCCCCGA GGTGTAGCAT TTAGCCAGGA TGGCTATTTG 
GTAGTGACTG ACTTCAATAA TCATCGGTTA TTAATAATCA AGCCAGATTG TCAGTCGGCA 
CATTTCTTGG GCACTGAGGG CACAGGCAAT GGGCAGTTTT TGCGTCCACA GGGTGTAGCA 
GTTGACCAGG AAGGGCGCAT CATTGTGGCA GACTCCAGAA ATCACAGAGT CCAGATATTT 
GAGCCTAATG GCAGCTTCCT GTGCAAGTTT GGAACTCAAG GAAGTGGCTT TGGTCAAATG 
GATCGCCCGT CTGGTATAGC TGTAACTCCC GATGGCACAA TTGTTGTGGT GGATTTTGGA 
AATAACCGCA TTCTAGCCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915430.1
CDS	909..3452
Translation	MASFPESDFQLCPLCKEMCVSSAPISSSSSSSSSSQVSTSSSSAGSAGGGTGLASAPPRRLHVLPCLHAFCRQCLEGRRSPGDSLQLRCPVCDHKVLISEAGMDALPSSTFLHLSNLLDAVVGAADEQQQSNGGRTASNRQRSASCSSSGLLRRAPPSQSEPRCSSCDDGNGASSHCLDCQENLCDNCLRAHQRVRLTKDHFIERFPASPCSSAASSAATTSSSSSSAFSLLPVYPERLYCQQHDEEIYKFGIRKRVYTLADLVLHFYCDSCSVPICRECTMGRHAGHSFVYLQEALQDSRALTIQLLADAQQGRQAIQLSLEQAQALAEQVEMKAKVVQSEIKAVTSRHKKALEERECELLWKVEKIRQVKAKSLYHQVEKLHQALNKLDSTINAVQQVLEEGCTMDILIARDRVLAQVQDVKNARGLLQLQEDDRIMFTPPDQALYLAIKSLGLVSSGAFAALTKATGEGLKRALQGKVASFTVIGYDHDGEARLSGGDLITVLVMGPDGNLFAAEVSDQLNGTYLVSYRPQLEGEHLISVMVCNQHIENSPFKVNVKSGRCYLGIGLPTLSFGGEGDHDGKLCRPWGVCVDREGYIIVADRSNNRVQIFKPCGTFHHKFGSLGSRPGQFDRPAGVACDNSRRIVVADKDNHRVQIFTFEGQFLLKFGEKGTKNGQFNYPWDVAVNSEGKILVSDTRNHRVQLFGPDGTFLNKYGFEGALWKHFDSPRGVAFSQDGYLVVTDFNNHRLLIIKPDCQSAHFLGTEGTGNGQFLRPQGVAVDQEGRIIVADSRNHRVQIFEPNGSFLCKFGTQGSGFGQMDRPSGIAVTPDGTIVVVDFGNNRILAF

Target ORF information:

RefSeq Version	XM_004915373.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915373.4

ORF Insert Sequence:

ATGGCTTCAT TCCCGGAGTC CGATTTCCAG CTGTGCCCCC TGTGTAAGGA GATGTGCGTG 
TCCTCTGCCC CCATCTCCTC CTCCAGCTCT TCCTCATCCT CCTCCCAGGT GTCCACCAGC 
AGCAGTAGCG CCGGCAGTGC AGGAGGAGGC ACCGGCTTGG CCTCGGCTCC CCCCCGCAGG 
CTGCACGTCT TGCCGTGCCT GCACGCCTTC TGTCGCCAGT GCCTGGAGGG GCGCCGGAGC 
CCCGGGGACT CTCTCCAGCT GCGCTGCCCG GTCTGTGACC ACAAGGTGCT CATCTCCGAG 
GCCGGCATGG ACGCGCTGCC CTCTTCTACC TTCTTGCACC TCAGCAACCT GCTGGACGCG 
GTGGTGGGCG CAGCCGACGA GCAGCAACAG AGTAACGGTG GCCGGACTGC TTCCAACAGA 
CAGCGTTCGG CTTCATGTTC TTCCTCCGGC CTCCTCCGCA GGGCGCCCCC CAGTCAGTCC 
GAACCGCGGT GCTCCTCATG CGACGATGGC AACGGGGCCA GCTCGCACTG CTTGGACTGC 
CAGGAGAACT TGTGCGACAA CTGCCTGAGG GCCCACCAGC GGGTCCGACT TACTAAGGAC 
CACTTCATAG AGCGCTTCCC CGCCTCCCCC TGCAGCTCCG CGGCTTCCAG TGCCGCCACT 
ACCTCGTCCT CTTCCAGCTC GGCCTTCTCC CTCCTGCCTG TGTACCCGGA GAGGCTCTAC 
TGCCAGCAGC ACGATGAGGA GATTTACAAA TTTGGAATAC GCAAGAGAGT GTATACCCTG 
GCGGATCTGG TCCTGCATTT CTACTGTGAC TCCTGCTCTG TTCCTATATG TCGTGAGTGC 
ACTATGGGCC GTCATGCTGG ACACAGCTTT GTCTACCTCC AGGAAGCTCT TCAGGACTCA 
AGGGCACTAA CTATTCAGCT ATTAGCAGAT GCCCAACAAG GCCGTCAAGC AATTCAGCTG 
AGTCTAGAAC AAGCTCAAGC ATTAGCAGAA CAAGTTGAGA TGAAAGCCAA AGTGGTGCAG 
TCTGAGATCA AAGCAGTCAC ATCTAGACAC AAGAAGGCAT TGGAAGAGAG AGAGTGTGAG 
CTGCTCTGGA AGGTTGAAAA GATACGTCAG GTGAAAGCAA AATCACTTTA TCACCAAGTT 
GAAAAACTCC ACCAGGCCCT TAATAAGTTG GACAGCACAA TAAATGCAGT GCAGCAAGTC 
TTGGAGGAAG GTTGTACTAT GGATATCCTA ATAGCTCGTG ATCGTGTTTT GGCTCAGGTG 
CAGGATGTAA AGAATGCTAG AGGCCTTTTA CAACTTCAAG AAGATGACAG GATTATGTTT 
ACTCCTCCTG ACCAAGCCTT ATACCTTGCC ATCAAATCCT TGGGTCTTGT CAGCAGTGGA 
GCTTTTGCAG CTTTGACTAA AGCCACTGGA GAAGGACTAA AGCGTGCCCT ACAGGGAAAA 
GTTGCATCAT TCACTGTTAT AGGATATGAC CATGATGGAG AAGCCCGCCT CTCAGGTGGG 
GACCTCATAA CAGTGTTGGT GATGGGTCCA GATGGGAATC TCTTTGCTGC TGAGGTCAGT 
GATCAGCTAA ATGGTACCTA CTTAGTGAGC TACCGGCCTC AACTGGAAGG GGAGCATCTT 
ATTTCAGTTA TGGTTTGCAA CCAACACATA GAAAACAGTC CCTTTAAAGT CAATGTAAAA 
TCTGGACGTT GCTACCTGGG TATTGGACTT CCTACCCTCT CTTTTGGTGG GGAGGGTGAT 
CATGATGGAA AACTTTGCCG CCCCTGGGGA GTTTGTGTTG ACAGGGAAGG TTACATAATT 
GTGGCTGATA GGAGCAACAA CCGTGTGCAA ATCTTTAAGC CATGTGGAAC CTTCCATCAC 
AAATTTGGCA GCCTTGGCTC TAGACCTGGA CAGTTTGATC GACCTGCTGG AGTAGCTTGC 
GACAATTCAC GCAGAATAGT CGTGGCAGAC AAAGACAACC ACCGTGTTCA GATATTTACA 
TTTGAAGGGC AATTCTTACT AAAGTTTGGT GAAAAGGGGA CCAAGAATGG CCAATTCAAC 
TACCCTTGGG ATGTGGCAGT TAATTCTGAG GGAAAGATCC TAGTTTCAGA CACCCGAAAC 
CACCGTGTCC AGCTGTTTGG TCCTGATGGG ACATTCCTTA ACAAGTATGG CTTCGAGGGT 
GCACTCTGGA AGCACTTTGA CTCTCCCCGA GGTGTAGCAT TTAGCCAGGA TGGCTATTTG 
GTAGTGACTG ACTTCAATAA TCATCGGTTA TTAATAATCA AGCCAGATTG TCAGTCGGCA 
CATTTCTTGG GCACTGAGGG CACAGGCAAT GGGCAGTTTT TGCGTCCACA GGGTGTAGCA 
GTTGACCAGG AAGGGCGCAT CATTGTGGCA GACTCCAGAA ATCACAGAGT CCAGATATTT 
GAGCCTAATG GCAGCTTCCT GTGCAAGTTT GGAACTCAAG GAAGTGGCTT TGGTCAAATG 
GATCGCCCGT CTGGTATAGC TGTAACTCCC GATGGCACAA TTGTTGTGGT GGATTTTGGA 
AATAACCGCA TTCTAGCCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim71 ( XM_002937846.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937892.1 Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa60087
Clone ID Related Accession (Same CDS sequence)	XM_002937846.5
Accession Version	XM_002937846.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase TRIM71 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937846.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCAT TCCCGGAGTC CGATTTCCAG CTGTGCCCCC TGTGTAAGGA GATGTGCGTG 
TCCTCTGCCC CCATCTCCTC CTCCAGCTCT TCCTCATCCT CCTCCCAGGT GTCCACCAGC 
AGCAGTAGCG CCGGCAGTGC AGGAGGAGGC ACCGGCTTGG CCTCGGCTCC CCCCCGCAGG 
CTGCACGTCT TGCCGTGCCT GCACGCCTTC TGTCGCCAGT GCCTGGAGGG GCGCCGGAGC 
CCCGGGGACT CTCTCCAGCT GCGCTGCCCG GTCTGTGACC ACAAGGTGCT CATCTCCGAG 
GCCGGCATGG ACGCGCTGCC CTCTTCTACC TTCTTGCACC TCAGCAACCT GCTGGACGCG 
GTGGTGGGCG CAGCCGACGA GCAGCAACAG AGTAACGGTG GCCGGACTGC TTCCAACAGA 
CAGCGTTCGG CTTCATGTTC TTCCTCCGGC CTCCTCCGCA GGGCGCCCCC CAGTCAGTCC 
GAACCGCGGT GCTCCTCATG CGACGATGGC AACGGGGCCA GCTCGCACTG CTTGGACTGC 
CAGGAGAACT TGTGCGACAA CTGCCTGAGG GCCCACCAGC GGGTCCGACT TACTAAGGAC 
CACTTCATAG AGCGCTTCCC CGCCTCCCCC TGCAGCTCCG CGGCTTCCAG TGCCGCCACT 
ACCTCGTCCT CTTCCAGCTC GGCCTTCTCC CTCCTGCCTG TGTACCCGGA GAGGCTCTAC 
TGCCAGCAGC ACGATGAGGA GGTCCTGCAT TTCTACTGTG ACTCCTGCTC TGTTCCTATA 
TGTCGTGAGT GCACTATGGG CCGTCATGCT GGACACAGCT TTGTCTACCT CCAGGAAGCT 
CTTCAGGACT CAAGGGCACT AACTATTCAG CTATTAGCAG ATGCCCAACA AGGCCGTCAA 
GCAATTCAGC TGAGTCTAGA ACAAGCTCAA GCATTAGCAG AACAAGTTGA GATGAAAGCC 
AAAGTGGTGC AGTCTGAGAT CAAAGCAGTC ACATCTAGAC ACAAGAAGGC ATTGGAAGAG 
AGAGAGTGTG AGCTGCTCTG GAAGGTTGAA AAGATACGTC AGGTGAAAGC AAAATCACTT 
TATCACCAAG TTGAAAAACT CCACCAGGCC CTTAATAAGT TGGACAGCAC AATAAATGCA 
GTGCAGCAAG TCTTGGAGGA AGGTTGTACT ATGGATATCC TAATAGCTCG TGATCGTGTT 
TTGGCTCAGG TGCAGGATGT AAAGAATGCT AGAGGCCTTT TACAACTTCA AGAAGATGAC 
AGGATTATGT TTACTCCTCC TGACCAAGCC TTATACCTTG CCATCAAATC CTTGGGTCTT 
GTCAGCAGTG GAGCTTTTGC AGCTTTGACT AAAGCCACTG GAGAAGGACT AAAGCGTGCC 
CTACAGGGAA AAGTTGCATC ATTCACTGTT ATAGGATATG ACCATGATGG AGAAGCCCGC 
CTCTCAGGTG GGGACCTCAT AACAGTGTTG GTGATGGGTC CAGATGGGAA TCTCTTTGCT 
GCTGAGGTCA GTGATCAGCT AAATGGTACC TACTTAGTGA GCTACCGGCC TCAACTGGAA 
GGGGAGCATC TTATTTCAGT TATGGTTTGC AACCAACACA TAGAAAACAG TCCCTTTAAA 
GTCAATGTAA AATCTGGACG TTGCTACCTG GGTATTGGAC TTCCTACCCT CTCTTTTGGT 
GGGGAGGGTG ATCATGATGG AAAACTTTGC CGCCCCTGGG GAGTTTGTGT TGACAGGGAA 
GGTTACATAA TTGTGGCTGA TAGGAGCAAC AACCGTGTGC AAATCTTTAA GCCATGTGGA 
ACCTTCCATC ACAAATTTGG CAGCCTTGGC TCTAGACCTG GACAGTTTGA TCGACCTGCT 
GGAGTAGCTT GCGACAATTC ACGCAGAATA GTCGTGGCAG ACAAAGACAA CCACCGTGTT 
CAGATATTTA CATTTGAAGG GCAATTCTTA CTAAAGTTTG GTGAAAAGGG GACCAAGAAT 
GGCCAATTCA ACTACCCTTG GGATGTGGCA GTTAATTCTG AGGGAAAGAT CCTAGTTTCA 
GACACCCGAA ACCACCGTGT CCAGCTGTTT GGTCCTGATG GGACATTCCT TAACAAGTAT 
GGCTTCGAGG GTGCACTCTG GAAGCACTTT GACTCTCCCC GAGGTGTAGC ATTTAGCCAG 
GATGGCTATT TGGTAGTGAC TGACTTCAAT AATCATCGGT TATTAATAAT CAAGCCAGAT 
TGTCAGTCGG CACATTTCTT GGGCACTGAG GGCACAGGCA ATGGGCAGTT TTTGCGTCCA 
CAGGGTGTAG CAGTTGACCA GGAAGGGCGC ATCATTGTGG CAGACTCCAG AAATCACAGA 
GTCCAGATAT TTGAGCCTAA TGGCAGCTTC CTGTGCAAGT TTGGAACTCA AGGAAGTGGC 
TTTGGTCAAA TGGATCGCCC GTCTGGTATA GCTGTAACTC CCGATGGCAC AATTGTTGTG 
GTGGATTTTG GAAATAACCG CATTCTAGCC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937892.1
CDS	906..3401
Translation	MASFPESDFQLCPLCKEMCVSSAPISSSSSSSSSSQVSTSSSSAGSAGGGTGLASAPPRRLHVLPCLHAFCRQCLEGRRSPGDSLQLRCPVCDHKVLISEAGMDALPSSTFLHLSNLLDAVVGAADEQQQSNGGRTASNRQRSASCSSSGLLRRAPPSQSEPRCSSCDDGNGASSHCLDCQENLCDNCLRAHQRVRLTKDHFIERFPASPCSSAASSAATTSSSSSSAFSLLPVYPERLYCQQHDEEVLHFYCDSCSVPICRECTMGRHAGHSFVYLQEALQDSRALTIQLLADAQQGRQAIQLSLEQAQALAEQVEMKAKVVQSEIKAVTSRHKKALEERECELLWKVEKIRQVKAKSLYHQVEKLHQALNKLDSTINAVQQVLEEGCTMDILIARDRVLAQVQDVKNARGLLQLQEDDRIMFTPPDQALYLAIKSLGLVSSGAFAALTKATGEGLKRALQGKVASFTVIGYDHDGEARLSGGDLITVLVMGPDGNLFAAEVSDQLNGTYLVSYRPQLEGEHLISVMVCNQHIENSPFKVNVKSGRCYLGIGLPTLSFGGEGDHDGKLCRPWGVCVDREGYIIVADRSNNRVQIFKPCGTFHHKFGSLGSRPGQFDRPAGVACDNSRRIVVADKDNHRVQIFTFEGQFLLKFGEKGTKNGQFNYPWDVAVNSEGKILVSDTRNHRVQLFGPDGTFLNKYGFEGALWKHFDSPRGVAFSQDGYLVVTDFNNHRLLIIKPDCQSAHFLGTEGTGNGQFLRPQGVAVDQEGRIIVADSRNHRVQIFEPNGSFLCKFGTQGSGFGQMDRPSGIAVTPDGTIVVVDFGNNRILAF

Target ORF information:

RefSeq Version	XM_002937846.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 71, E3 ubiquitin protein ligase (trim71), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937846.5

ORF Insert Sequence:

ATGGCTTCAT TCCCGGAGTC CGATTTCCAG CTGTGCCCCC TGTGTAAGGA GATGTGCGTG 
TCCTCTGCCC CCATCTCCTC CTCCAGCTCT TCCTCATCCT CCTCCCAGGT GTCCACCAGC 
AGCAGTAGCG CCGGCAGTGC AGGAGGAGGC ACCGGCTTGG CCTCGGCTCC CCCCCGCAGG 
CTGCACGTCT TGCCGTGCCT GCACGCCTTC TGTCGCCAGT GCCTGGAGGG GCGCCGGAGC 
CCCGGGGACT CTCTCCAGCT GCGCTGCCCG GTCTGTGACC ACAAGGTGCT CATCTCCGAG 
GCCGGCATGG ACGCGCTGCC CTCTTCTACC TTCTTGCACC TCAGCAACCT GCTGGACGCG 
GTGGTGGGCG CAGCCGACGA GCAGCAACAG AGTAACGGTG GCCGGACTGC TTCCAACAGA 
CAGCGTTCGG CTTCATGTTC TTCCTCCGGC CTCCTCCGCA GGGCGCCCCC CAGTCAGTCC 
GAACCGCGGT GCTCCTCATG CGACGATGGC AACGGGGCCA GCTCGCACTG CTTGGACTGC 
CAGGAGAACT TGTGCGACAA CTGCCTGAGG GCCCACCAGC GGGTCCGACT TACTAAGGAC 
CACTTCATAG AGCGCTTCCC CGCCTCCCCC TGCAGCTCCG CGGCTTCCAG TGCCGCCACT 
ACCTCGTCCT CTTCCAGCTC GGCCTTCTCC CTCCTGCCTG TGTACCCGGA GAGGCTCTAC 
TGCCAGCAGC ACGATGAGGA GGTCCTGCAT TTCTACTGTG ACTCCTGCTC TGTTCCTATA 
TGTCGTGAGT GCACTATGGG CCGTCATGCT GGACACAGCT TTGTCTACCT CCAGGAAGCT 
CTTCAGGACT CAAGGGCACT AACTATTCAG CTATTAGCAG ATGCCCAACA AGGCCGTCAA 
GCAATTCAGC TGAGTCTAGA ACAAGCTCAA GCATTAGCAG AACAAGTTGA GATGAAAGCC 
AAAGTGGTGC AGTCTGAGAT CAAAGCAGTC ACATCTAGAC ACAAGAAGGC ATTGGAAGAG 
AGAGAGTGTG AGCTGCTCTG GAAGGTTGAA AAGATACGTC AGGTGAAAGC AAAATCACTT 
TATCACCAAG TTGAAAAACT CCACCAGGCC CTTAATAAGT TGGACAGCAC AATAAATGCA 
GTGCAGCAAG TCTTGGAGGA AGGTTGTACT ATGGATATCC TAATAGCTCG TGATCGTGTT 
TTGGCTCAGG TGCAGGATGT AAAGAATGCT AGAGGCCTTT TACAACTTCA AGAAGATGAC 
AGGATTATGT TTACTCCTCC TGACCAAGCC TTATACCTTG CCATCAAATC CTTGGGTCTT 
GTCAGCAGTG GAGCTTTTGC AGCTTTGACT AAAGCCACTG GAGAAGGACT AAAGCGTGCC 
CTACAGGGAA AAGTTGCATC ATTCACTGTT ATAGGATATG ACCATGATGG AGAAGCCCGC 
CTCTCAGGTG GGGACCTCAT AACAGTGTTG GTGATGGGTC CAGATGGGAA TCTCTTTGCT 
GCTGAGGTCA GTGATCAGCT AAATGGTACC TACTTAGTGA GCTACCGGCC TCAACTGGAA 
GGGGAGCATC TTATTTCAGT TATGGTTTGC AACCAACACA TAGAAAACAG TCCCTTTAAA 
GTCAATGTAA AATCTGGACG TTGCTACCTG GGTATTGGAC TTCCTACCCT CTCTTTTGGT 
GGGGAGGGTG ATCATGATGG AAAACTTTGC CGCCCCTGGG GAGTTTGTGT TGACAGGGAA 
GGTTACATAA TTGTGGCTGA TAGGAGCAAC AACCGTGTGC AAATCTTTAA GCCATGTGGA 
ACCTTCCATC ACAAATTTGG CAGCCTTGGC TCTAGACCTG GACAGTTTGA TCGACCTGCT 
GGAGTAGCTT GCGACAATTC ACGCAGAATA GTCGTGGCAG ACAAAGACAA CCACCGTGTT 
CAGATATTTA CATTTGAAGG GCAATTCTTA CTAAAGTTTG GTGAAAAGGG GACCAAGAAT 
GGCCAATTCA ACTACCCTTG GGATGTGGCA GTTAATTCTG AGGGAAAGAT CCTAGTTTCA 
GACACCCGAA ACCACCGTGT CCAGCTGTTT GGTCCTGATG GGACATTCCT TAACAAGTAT 
GGCTTCGAGG GTGCACTCTG GAAGCACTTT GACTCTCCCC GAGGTGTAGC ATTTAGCCAG 
GATGGCTATT TGGTAGTGAC TGACTTCAAT AATCATCGGT TATTAATAAT CAAGCCAGAT 
TGTCAGTCGG CACATTTCTT GGGCACTGAG GGCACAGGCA ATGGGCAGTT TTTGCGTCCA 
CAGGGTGTAG CAGTTGACCA GGAAGGGCGC ATCATTGTGG CAGACTCCAG AAATCACAGA 
GTCCAGATAT TTGAGCCTAA TGGCAGCTTC CTGTGCAAGT TTGGAACTCA AGGAAGTGGC 
TTTGGTCAAA TGGATCGCCC GTCTGGTATA GCTGTAACTC CCGATGGCAC AATTGTTGTG 
GTGGATTTTG GAAATAACCG CATTCTAGCC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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trim71 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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