Trim71 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trim71
Entrez Gene ID	301042
Full Name	tripartite motif containing 71
Synonyms	RGD1566388
General protein information	Preferred Names tripartite motif containing 71 Names E3 ubiquitin-protein ligase TRIM71 B-box zinc finger, Filamin and NHL repeat containing protein (123.8 kD) (lin-41) Drosophila dappled/ vertebrate TRipartite Motif protein related RING-type E3 ubiquitin transferase TRIM71 protein lin-41 homolog tripartite motif containing 71, E3 ubiquitin protein ligase tripartite motif-containing protein 71
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32

mRNA and Protein(s)

mRNA	Protein	Name
NM_001191801.1	NP_001178730.1	E3 ubiquitin-protein ligase TRIM71

Pathways from BioSystems

Homologies

	TRIM71	XP_001098706.1
Bos taurus (cattle)	TRIM71	XP_002707823.1
Gallus gallus (chicken)	TRIM71	NP_001032352.2
Pan troglodytes (chimpanzee)	TRIM71	XP_516352.3
Rattus norvegicus (Norway rat)	Trim71	NP_001178730.1
Danio rerio (zebrafish)	trim71	XP_690252.5
Caenorhabditis elegans (roundworm)	lin-41	NP_001020998.1
Mus musculus (house mouse)	Trim71	NP_001035968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005125	XP_314006.4
Homo sapiens (human)	TRIM71	NP_001034200.1
Canis lupus familiaris (dog)	TRIM71	XP_534214.3
Drosophila melanogaster (fruit fly)	wech	NP_001137614.1
Xenopus tropicalis (tropical clawed frog)	LOC100486898	XP_004915429.1

Gene Ontology
Bibliography

Related articles in PubMed

A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis.
Treiber T, Treiber N, Plessmann U, Harlander S, Dai? JL, Eichner N, Lehmann G, Schall K, Urlaub H, Meister G
Molecular cell66(2)270-284.e13(2017 Apr)

The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function.
Loedige I, Gaidatzis D, Sack R, Meister G, Filipowicz W
Nucleic acids research41(1)518-32(2013 Jan)

Trim71 cooperates with microRNAs to repress Cdkn1a expression and promote embryonic stem cell proliferation.
Chang HM, Martinez NJ, Thornton JE, Hagan JP, Nguyen KD, Gregory RI
Nature communications3923(2012 Jun)

The ubiquitin ligase mLin41 temporally promotes neural progenitor cell maintenance through FGF signaling.
Chen J, Lai F, Niswander L
Genes & development26(8)803-15(2012 Apr)

The let-7 target gene mouse lin-41 is a stem cell specific E3 ubiquitin ligase for the miRNA pathway protein Ago2.
Rybak A, Fuchs H, Hadian K, Smirnova L, Wulczyn EA, Michel G, Nitsch R, Krappmann D, Wulczyn FG
Nature cell biology11(12)1411-20(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Trim71 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trim71 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07691	NM_001191801.1 Latest version!	Rattus norvegicus tripartite motif containing 71 (Trim71), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trim71 ( NM_001191801.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178730.1 Rattus norvegicus tripartite motif containing 71 (Trim71), mRNA.

Price & Availability↑

CloneID	ORa07691
Clone ID Related Accession (Same CDS sequence)	NM_001191801.1
Accession Version	NM_001191801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase TRIM71
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07071570.1 and AABR07071568.1. On Jul 17, 2010 this sequence version replaced XM_236676.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689604, SAMN01906349 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTCGT TCCCTGAGAC CGACTTCCAG ATCTGCCTGC TGTGCAAGGA GATGTGCGGC 
TCGCCCGCGC CTCTCTCCTC CAGCTCGTCC GCCTCTTCAT CGTCCTCGCA GACGTCCACG 
TCGTCGGCGG GCGGCGGCGG CCCCGGGGCG GCGGCGCGGC GCCTGCACGT CCTGCCCTGC 
CTGCACGCCT TCTGCCGGCC GTGCCTCGAG GCGCACCGGC TCCCGGCTCC CGGTGGCGCC 
GGCCCCGCCG AGGCGCTGAA GCTGCGCTGT CCTGTGTGTG ACCAGAAGGT GGTCCTGGCC 
GAGGCGGCAG GCATGGACGC GCTGCCGTCG TCCGCCTTCC TGCTCAGCAA CCTGCTGGAC 
GCCGTGGTGG CCACCGCCGA CGAGCCGCCG CCCAAGAACG GGCGCGCGGG TGGCGGCCCC 
GGGGGCGCGG GCGGCCACAG CAATCACCGG CACCACGCGC ACCACCCGGC CCAGCGCGCC 
GCCGCTCCAG CGCCGCAGCC GCCGCCCGGC CCGGCCGCTT CTCCAGGGTC GCTGTTGTTG 
CGCCGGCCGC ACGGCTGCAG CTCCTGCGAC GAGGGCAATG CTGCCTCGTC GCGCTGCCTC 
GACTGCCAGG AACACCTGTG CGACAACTGT GTCCGCGCGC ACCAGCGCGT ACGCCTCACC 
AAGGACCACT ACATCGAGCG CGGCCCGCCG GGGCCTGCGG CCGCCTCCGC GGCGCAGCAG 
CTGGGGCTCG GGCCGCCCTT CGCAGGCGCG CCCTTCTCCA TCCTCTCGGT GTTCCCGGAG 
CGCCTCGGCT TCTGCCAACA CCACGACGAC GAGGTACTAC ATCTGTACTG CGACACCTGC 
TCCGTGCCCA TCTGCAGAGA GTGCACACTG GGCCGCCATG GCGGCCACAG CTTCGCCTAC 
CTCCAGGACG CCCTACAGGA CTCACGGGCA CTCACCATCC AGCTGCTGGC CGACGCCCAG 
CAGGGCCGCC AGGCCATTCA GCTGAGCATT GAGCAGGCGC AGACTGTGGC AGAACAAGTG 
GAGATGAAGG CCAAGGTGGT CCAGTCGGAG GTCAAGGCCG TCACGGCGAG ACACAAGAAA 
GCTCTGGAGG AGCGTGAGTG CGAGCTGCTG TGGAAGGTGG AGAAGATCCG GCAGGTGAAG 
GCTAAGTCTC TCTACCTGCA GGTGGAGAAG CTGCGGCAGA ATCTCAACAA GCTGGAGAGC 
ACCATCAGCG CCGTCCAGCA GGTCCTAGAA GAGGGCCGAG CCCTGGACAT CCTGCTGGCC 
CGAGACCGGA TGCTGGCTCA GGTGCAGGAG CTCAAGACCA TCCGGGGCCT CCTGCAGCCC 
CAGGAAGATG ACCGAGTTAT GTTCACACCC CCCGACCAGG CCCTGTACCT TGCCCTCAAG 
TCTTTTGGCT TTGTCAGCAG TGGGGCCTTT GCCCCACTCA CGAAGGCCGC GGGAGATGGC 
ATCAAGCGAG CCCTTCAGGG AAAGGTGGCC TCCTTCACTG TCATGGGCTA TGACCACAAT 
GGTGAGCCCC GCCTCTCAGG AGGTGACCTG ATGTCGGTGG TGGTCCTTGG CCCTGATGGA 
AACCTGTTTG GTGCAGAAGT GAGCGACCAG CAGAATGGGA CCTACGTCGT GAGCTACAGG 
CCCCAGCTTG AGGGTGAACA CTTGGTATCA GTGACGCTGT ATAACCAGCA CATCGAGAAC 
AGCCCCTTCA AGGTAGTGGT CAAGTCGGGC CGCAGCTATG TGGGCATCGG GCTCCCAGTG 
TTGAGCTTTG GCAGCGAGGG TGATGGCGAG GGTAAGCTCT GCAGGCCCTG GGGCGTGAGT 
GTGGATAAGG AGGGCTACAT CATCGTGGCT GACCGCAGCA ATAACAGAAT TCAGGTGTTT 
AAGCCCTGTG GGTCTTTCCA CCACAAGTTT GGTACCCTAG GATCTCGGCC CGGGCAGTTT 
GACCGACCGG CGGGGGTGGC CTGCGACGCC TCCCGCAGGA TCGTGGTGGC CGACAAAGAC 
AATCATCGCA TCCAGATCTT CACCTTCGAG GGCCAGTTCC TTCTGAAGTT TGGCGAGAAA 
GGAACCAAGA ATGGGCAGTT TAACTACCCG TGGGATGTAG CGGTCAACTC TGAGGGCAAG 
ATCCTGGTTT CAGACACCCG AAACCACCGC ATCCAGCTGT TCGGACCCGA CGGCGTCTTC 
CTGAATAAGT ATGGCTTCGA GGGATCTCTC TGGAAGCACT TCGACTCCCC ACGGGGCGTG 
GCTTTCAACC ATGAAGGTCA CCTGGTAGTC ACTGATTTCA ACAACCATCG GCTCCTGGTT 
ATCCACCCCG ATTGCCAGTC TGCCCGCTTC CTGGGCTCTG AGGGCACTGG CAATGGGCAG 
TTCCTGCGAC CACAGGGTGT GGCTGTAGAC CAGGAAGGGC GAATCATTGT CGCCGATTCC 
AGAAATCACC GTGTGCAGAT GTTTGAGGCC AACGGCAGCT TCCTGTGCAA GTTCGGTGCT 
CAAGGCAGCG GCTTCGGGCA GATGGACCGC CCCTCAGGTA TCGCGGTCAC CCCGGAAGGA 
TTGATCGTCG TGGTGGACTT TGGCAACAAT CGAATCCTCA TCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178730.1
CDS	1..2568
Translation	MASFPETDFQICLLCKEMCGSPAPLSSSSSASSSSSQTSTSSAGGGGPGAAARRLHVLPCLHAFCRPCLEAHRLPAPGGAGPAEALKLRCPVCDQKVVLAEAAGMDALPSSAFLLSNLLDAVVATADEPPPKNGRAGGGPGGAGGHSNHRHHAHHPAQRAAAPAPQPPPGPAASPGSLLLRRPHGCSSCDEGNAASSRCLDCQEHLCDNCVRAHQRVRLTKDHYIERGPPGPAAASAAQQLGLGPPFAGAPFSILSVFPERLGFCQHHDDEVLHLYCDTCSVPICRECTLGRHGGHSFAYLQDALQDSRALTIQLLADAQQGRQAIQLSIEQAQTVAEQVEMKAKVVQSEVKAVTARHKKALEERECELLWKVEKIRQVKAKSLYLQVEKLRQNLNKLESTISAVQQVLEEGRALDILLARDRMLAQVQELKTIRGLLQPQEDDRVMFTPPDQALYLALKSFGFVSSGAFAPLTKAAGDGIKRALQGKVASFTVMGYDHNGEPRLSGGDLMSVVVLGPDGNLFGAEVSDQQNGTYVVSYRPQLEGEHLVSVTLYNQHIENSPFKVVVKSGRSYVGIGLPVLSFGSEGDGEGKLCRPWGVSVDKEGYIIVADRSNNRIQVFKPCGSFHHKFGTLGSRPGQFDRPAGVACDASRRIVVADKDNHRIQIFTFEGQFLLKFGEKGTKNGQFNYPWDVAVNSEGKILVSDTRNHRIQLFGPDGVFLNKYGFEGSLWKHFDSPRGVAFNHEGHLVVTDFNNHRLLVIHPDCQSARFLGSEGTGNGQFLRPQGVAVDQEGRIIVADSRNHRVQMFEANGSFLCKFGAQGSGFGQMDRPSGIAVTPEGLIVVVDFGNNRILIF

Target ORF information:

RefSeq Version	NM_001191801.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tripartite motif containing 71 (Trim71), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191801.1

ORF Insert Sequence:

ATGGCTTCGT TCCCTGAGAC CGACTTCCAG ATCTGCCTGC TGTGCAAGGA GATGTGCGGC 
TCGCCCGCGC CTCTCTCCTC CAGCTCGTCC GCCTCTTCAT CGTCCTCGCA GACGTCCACG 
TCGTCGGCGG GCGGCGGCGG CCCCGGGGCG GCGGCGCGGC GCCTGCACGT CCTGCCCTGC 
CTGCACGCCT TCTGCCGGCC GTGCCTCGAG GCGCACCGGC TCCCGGCTCC CGGTGGCGCC 
GGCCCCGCCG AGGCGCTGAA GCTGCGCTGT CCTGTGTGTG ACCAGAAGGT GGTCCTGGCC 
GAGGCGGCAG GCATGGACGC GCTGCCGTCG TCCGCCTTCC TGCTCAGCAA CCTGCTGGAC 
GCCGTGGTGG CCACCGCCGA CGAGCCGCCG CCCAAGAACG GGCGCGCGGG TGGCGGCCCC 
GGGGGCGCGG GCGGCCACAG CAATCACCGG CACCACGCGC ACCACCCGGC CCAGCGCGCC 
GCCGCTCCAG CGCCGCAGCC GCCGCCCGGC CCGGCCGCTT CTCCAGGGTC GCTGTTGTTG 
CGCCGGCCGC ACGGCTGCAG CTCCTGCGAC GAGGGCAATG CTGCCTCGTC GCGCTGCCTC 
GACTGCCAGG AACACCTGTG CGACAACTGT GTCCGCGCGC ACCAGCGCGT ACGCCTCACC 
AAGGACCACT ACATCGAGCG CGGCCCGCCG GGGCCTGCGG CCGCCTCCGC GGCGCAGCAG 
CTGGGGCTCG GGCCGCCCTT CGCAGGCGCG CCCTTCTCCA TCCTCTCGGT GTTCCCGGAG 
CGCCTCGGCT TCTGCCAACA CCACGACGAC GAGGTACTAC ATCTGTACTG CGACACCTGC 
TCCGTGCCCA TCTGCAGAGA GTGCACACTG GGCCGCCATG GCGGCCACAG CTTCGCCTAC 
CTCCAGGACG CCCTACAGGA CTCACGGGCA CTCACCATCC AGCTGCTGGC CGACGCCCAG 
CAGGGCCGCC AGGCCATTCA GCTGAGCATT GAGCAGGCGC AGACTGTGGC AGAACAAGTG 
GAGATGAAGG CCAAGGTGGT CCAGTCGGAG GTCAAGGCCG TCACGGCGAG ACACAAGAAA 
GCTCTGGAGG AGCGTGAGTG CGAGCTGCTG TGGAAGGTGG AGAAGATCCG GCAGGTGAAG 
GCTAAGTCTC TCTACCTGCA GGTGGAGAAG CTGCGGCAGA ATCTCAACAA GCTGGAGAGC 
ACCATCAGCG CCGTCCAGCA GGTCCTAGAA GAGGGCCGAG CCCTGGACAT CCTGCTGGCC 
CGAGACCGGA TGCTGGCTCA GGTGCAGGAG CTCAAGACCA TCCGGGGCCT CCTGCAGCCC 
CAGGAAGATG ACCGAGTTAT GTTCACACCC CCCGACCAGG CCCTGTACCT TGCCCTCAAG 
TCTTTTGGCT TTGTCAGCAG TGGGGCCTTT GCCCCACTCA CGAAGGCCGC GGGAGATGGC 
ATCAAGCGAG CCCTTCAGGG AAAGGTGGCC TCCTTCACTG TCATGGGCTA TGACCACAAT 
GGTGAGCCCC GCCTCTCAGG AGGTGACCTG ATGTCGGTGG TGGTCCTTGG CCCTGATGGA 
AACCTGTTTG GTGCAGAAGT GAGCGACCAG CAGAATGGGA CCTACGTCGT GAGCTACAGG 
CCCCAGCTTG AGGGTGAACA CTTGGTATCA GTGACGCTGT ATAACCAGCA CATCGAGAAC 
AGCCCCTTCA AGGTAGTGGT CAAGTCGGGC CGCAGCTATG TGGGCATCGG GCTCCCAGTG 
TTGAGCTTTG GCAGCGAGGG TGATGGCGAG GGTAAGCTCT GCAGGCCCTG GGGCGTGAGT 
GTGGATAAGG AGGGCTACAT CATCGTGGCT GACCGCAGCA ATAACAGAAT TCAGGTGTTT 
AAGCCCTGTG GGTCTTTCCA CCACAAGTTT GGTACCCTAG GATCTCGGCC CGGGCAGTTT 
GACCGACCGG CGGGGGTGGC CTGCGACGCC TCCCGCAGGA TCGTGGTGGC CGACAAAGAC 
AATCATCGCA TCCAGATCTT CACCTTCGAG GGCCAGTTCC TTCTGAAGTT TGGCGAGAAA 
GGAACCAAGA ATGGGCAGTT TAACTACCCG TGGGATGTAG CGGTCAACTC TGAGGGCAAG 
ATCCTGGTTT CAGACACCCG AAACCACCGC ATCCAGCTGT TCGGACCCGA CGGCGTCTTC 
CTGAATAAGT ATGGCTTCGA GGGATCTCTC TGGAAGCACT TCGACTCCCC ACGGGGCGTG 
GCTTTCAACC ATGAAGGTCA CCTGGTAGTC ACTGATTTCA ACAACCATCG GCTCCTGGTT 
ATCCACCCCG ATTGCCAGTC TGCCCGCTTC CTGGGCTCTG AGGGCACTGG CAATGGGCAG 
TTCCTGCGAC CACAGGGTGT GGCTGTAGAC CAGGAAGGGC GAATCATTGT CGCCGATTCC 
AGAAATCACC GTGTGCAGAT GTTTGAGGCC AACGGCAGCT TCCTGTGCAA GTTCGGTGCT 
CAAGGCAGCG GCTTCGGGCA GATGGACCGC CCCTCAGGTA TCGCGGTCAC CCCGGAAGGA 
TTGATCGTCG TGGTGGACTT TGGCAACAAT CGAATCCTCA TCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis.
Molecular cell66(2)270-284.e13(2017 Apr)
Treiber T,Treiber N,Plessmann U,Harlander S,Dai? JL,Eichner N,Lehmann G,Schall K,Urlaub H,Meister G

The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function.
Nucleic acids research41(1)518-32(2013 Jan)
Loedige I,Gaidatzis D,Sack R,Meister G,Filipowicz W

Trim71 cooperates with microRNAs to repress Cdkn1a expression and promote embryonic stem cell proliferation.
Nature communications3923(2012 Jun)
Chang HM,Martinez NJ,Thornton JE,Hagan JP,Nguyen KD,Gregory RI

The ubiquitin ligase mLin41 temporally promotes neural progenitor cell maintenance through FGF signaling.
Genes & development26(8)803-15(2012 Apr)
Chen J,Lai F,Niswander L

The let-7 target gene mouse lin-41 is a stem cell specific E3 ubiquitin ligase for the miRNA pathway protein Ago2.
Nature cell biology11(12)1411-20(2009 Dec)
Rybak A,Fuchs H,Hadian K,Smirnova L,Wulczyn EA,Michel G,Nitsch R,Krappmann D,Wulczyn FG

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Trim71 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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