ALS2 cDNA ORF clone, Gallus gallus(chicken)

The following ALS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa38345 XM_015289455.2
Latest version!
Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa10920 XM_015289451.2
Latest version!
Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa10920 XM_015289453.2
Latest version!
Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa10920 XM_004942618.3
Latest version!
Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa10920 XM_421940.6
Latest version!
Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa10920 XM_015289452.2
Latest version!
Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa10920 XM_015289454.1
Latest version!
Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa10920 XM_004942617.3
Latest version!
Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OGa10920 XM_015289451.1 Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa10920 XM_015289452.1 Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa38345 XM_015289455.1 Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa10920 XM_421940.5 Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa10920 XM_004942617.2 Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa10920 XM_004942618.2 Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa10920 XM_015289453.1 Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa38345
    Clone ID Related Accession (Same CDS sequence) XM_015289455.1 , XM_015289455.2
    Accession Version XM_015289455.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product alsin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTGGGGGA ACAATTCTTC TGGACAGTGT GCAGTTGCTA ATCAACCATA TGTGCCGGAA 
    CCACAACCTA TCAGTATTTC TGATTCTGAA ACCAGTCCTC TACTATCAGT AAGGGTTTTG
    CAGCTAGCAT GTGGAGAAGA GCACACGTTG GCACTATCAC TAAGCAGAGA GATATGGGCT
    TGGGGGAGTG GCTGCCAGCT AGGTCTTATA ACAACAACTT TCCCAGTGAC AAAGCCACAG
    AAGGTAGAGC ACCTGGCAGG ACGTGTTGTG CTCCAGATTG CTTGTGGAGC CTACCACAGC
    CTGGCACTGG TACAATGTCT CCCTGTGCAG GAGATGAAGC CTATTCCAGA GAGGTGCAAT
    CATTGCAGTC AACTCCTCAT TACCATGACA GACAAGGAAG ATCATGTAAT CATTTCCGAT
    AGTCACTGTT GCCCACTGGG CGTAGCGCTG GCTGATAAAC AACCAGAAAA CCATGCCTTC
    TGTTCTTCGT TGGAAAACCT GGAAGGAAAA AGTGTAACAG GTAGTCAGAG TGAAGACTGT
    AAGAAACCTC TTGTGGAAGA GCATACATTT GTTGCTTTAG AATCTGATGG AGCAAGCTTA
    CTTTCCAGCA ACAATGGACA GGAAGACAGT GGAATTTCTA GCCCTGGAAA TATTCTGTGT
    ACTGACAAAA AAGAAGTGAA AGAATACTTA ATCGGTTTAT CTGATCACAC ATTAAATGAG
    AGCCTGGAGA AAAATATCTG CACAGAATCT GAGCAGCCTT CTCAAGGAGA ACAAATAGGT
    GCTTGTATCC CTGGTCCTCC AGGTACGAGT GCGTCTGCCT TAAACAGCCT GGTGGCCTCC
    TGTGCATCAG CAGTTGGTGT GAGGGTAGCT GCTACATATG AAGCTGGAGC GTTGTCTCTA
    AAGAAAGTAA TGAACTTCTA TGGCACACCT GCAAATGAGC CAGGCCCTCA GCCTGGCAGT
    GGTTCCACAG GGCCTGAAGC TCTGAAAGAC AGTCGAGAAG AGCAGGTCAA ACTGGAATCA
    ATGCAGGGCA AGAAGAGTTC CAGTTTAGTA GATATTAGAG AGGAGGAATC AGAAGGAGGA
    AGTCGAAGAC TTTCCCTGCC TGGCCTGTTA TCACAAGTTT CTCCTAGGCT CTTACGGAAG
    GTAGGACGGG CAAAAATGAG GACTGTGGCT CTGACTCCAA CCTACAGTGG TGAGGCAGAT
    GCTCTTTTAC CATCTTTAAG AACAGAGGTA TGGAGCTGGG GAAGGGGGAA GGATGGACAG
    CTAGGACATG GTGATGTTCT ACCAAGGCTT CAGCCACTGT GTGTGAAAAG TTTAGATGGT
    AAAGAAGTGA TTCAACTCTC TGCTGGTGGT CATCATTCCT TAGCACTCAC TGCCAAATCC
    CAGGTGTATT CATGGGGCAG TAATATCTTT GGTCAGCTTG GTCACACGAA CTCCCCAACA
    ACAGTCCCTC GACTTGCAAA GGTGTGTGGT GATGGTGTTT GGAGTACAGC GGCAGGATCA
    GATTATTCTC TCTTCTTAGC AGATGAGGAA GACTTCCAAC CTGGATTATA TTATTGTGGT
    CAAGAGACAG CAACAGATAA TTTGAGTGAA AATAATTGTA GTAAGAATCC AGTTTTATTA
    CTATCCTGTA GTAAGATAGG ATATATAAGC AATGTGATAG CTGGTGGAGA CAACTGTTTG
    GTCTTGGTAG ACAAAAACAT AATGGGATAT ATTGCCAGTT TGCATGAGTT GGCTTCTACT
    GAGAGAAGGT TTTATTTCAG ACTGAGTGAG ATCAAATCTC AGGTTCTTAG ACCTCTTCTA
    GGTTTAGATA ATTTGGGCAA TGCAAATACA ATCCAGTTGT TACAAGAAAT GGCAAGCAGG
    TTCAGCAAGC TGTGCTATCT CATCGGTCAA CATGGAGCTT CTTTAAGTAG CTTTCTTCAT
    GGAGTTAAAG AAGCCAGGAG TTTGGCCATA CTGAAGCATT CCAGTCTCTT TTTGGATAGT
    TACACTGAAT ACTGTACTTC AATAACAAAT TTCCTGGTAA TGGGAGGATT TATGCTTCTC
    GCAAAGCCTG CAATAGACTT CTTGAGTAAA AGTCAGGAGC TGTTACAGAA ACTGTCTGAG
    TATAATGAGG AAAACCCGCA ACTAGTGGAT GTTCTAAATG CTCTGTTTTT CATGCCATTT
    GGACGACTTC ATGATTATGC CAGAACCTTG CTAAAGCTTG CCACGTGCTT TGAAGTGGCA
    TCTCCAGAAT ACCAGAAGCT ACAAGATTCT AGCTCTTGTT ATGAGAGCCT TGCTGTCCAT
    CTCGGCAGAA AAAGGAAAGA AGCAGAATAC ACACTCAGCT TCTGGAAGAC GTTTCCTGGG
    AAAATGACGG ATTCTTTGCG GAAGCCAGAC CGTCGTTTAA TCTGTGAAAG CAGCAATAGG
    GGGTTGTCCC TACAGCATGC TGGAAGATTC TCTGTGAACT GGTTTATCCT TTTTAACGAT
    GCTCTGGTGC ACGCTCAGTT TTCAACGCAC CATATTTTTG CTTTGTCCAC GCTGTGGGTA
    GAAGCTCTTT CAGAAGAAGC TGGTAATGTG AATGGATTGA AGATTACAAC ACCAGAAGAA
    CAGTTTGTTC TGCTTTCTTC AACACCACAG GAAAAGACGA AGTGGCTGAG GGCAATAAGT
    CAAGCAGTGG ATCAGGCCCT TAAAGGGACT TCTGATTTAA TTCTATATGG AGCTGGTGGG
    AATGTTCCAA GACAGGAGCC TCCTATTTCT CGCAGTGCCA AATACGCCTT CTATAAGGAC
    CCTCGATTTA AAGATGCTGT TTATGATGGG AGATGGCTCT CAGGAAAACC CCATGGCAGA
    GGGATCCTAA AGTGGGCTGA TGGAAGAATG TACTCAGGGA CCTTCCGAAC TGGACTGGAG
    GATGGGTATG GCGAATACAG AGTGCCAAAT AAAGCCTTGA ATAAAGATGA CCACTACGTG
    GGGTACTGGA AGGAAGGCAA AATGTGTGGG CATGGAGTCT ACAGCTATGC TACTGGTGAA
    GTATATGAAG GATGCTTTCA AGATAATATG CGTCACGGCC ATGGCCTGCT GCGCAGTGGG
    AAGCTTACCT CTTCTTCTCC TAGTATGTTC ATTGGACAGT GGACAATGGA TAAGAAGAGT
    GGTTATGGTG TTTTTGATGA TATTACAAGA GGAGAAAAGT ATATGGGAAT GTGGCAAGAT
    GACCTTTGCC AAGGCAATGG TGTTGTGGTT ACTCAGTTTG GATTGTATTA TGAGGGAGCC
    TTCAGCACTA ACAAAATGAT GGGGACTGGC ATTCTGCTTT CTGAGGATGA TACAGTTTAT
    GAAGGGGAAT TTTCAGATAA CTGGACTCTG AGTGGAAAGG GAACACTGAC CTTGCCAAAT
    GGAGATTATA TTGAAGGTCT CTTCAGTGGA GAGTGGGGAT CTGGAATAAA AATCACAGGA
    ACCTACTTCA AACCTAGTTT ATATGAGAGT GAGAAGGATA AGCCTAAAGC ATTGCGGAAA
    CTTGGGATCC TGGCAGTGCC TCCTGATGAG AAATGGAAGG CTGTATTTGA AGAATGTTGG
    AACCAGCTTG GCTGTGAGAG TCCAGGCGAG GGTGATAGAT GGAAGGCTTG GGACAACATT
    GCAGTGGCTC TGACCACCAA CCGACGGCAA CACAAGGACA GTCCAGAATT ACTGAGCCGT
    TCTCATACTC AGACACTGGA AAGTCTAGAA TTTATTCCGC AACACGTTGG TGCCTTCACC
    TTGGAAAAAT ATGAAAATAT CAAAAAATAT TTGATAAAGG CATGTGATAC TCCTCTCCAT
    CCGTTGGGCA AGCTTGTGGA AACACTAGTA GCTGTCTACA GAATGACTTA TGTTGGAGTA
    GGAGCTAATC GACGATTACT GCAGGAGGCA GTAAGAGAGA TCAAGTCCTA CCTCAAACGC
    ATCTTCCAAT TGGTTAGGTT CTTGTTCCCT GATCTTCCGG AAGAGGGCAG CACAATTCCA
    TTTCAGCCAA CGGAAGTAAA GGGAAGGCGT TCATTCTGCA GTGGGAAATC TGACTCCAGA
    TCAGAGTCCC CTGAGCCAGG CTATGTGGTA ACCAGTTTTG GCTTGCTACT CCCTGTACTA
    CTGCCACGTC TCTATCCACC TCTGTTTATG TTATATGCCT TAGACAACGA GCGTGAGGAA
    GATATTTACT GGGAGTGTGT CTTACGTCTA AACAAACAAA CTGATATGGC TCTTCTAAGT
    TTTCTTGGAG TGCAAGCGAA ATTCTGGCCA GGAACTGTAT CCATCCTTGG TGAGAGTAGA
    AAGGTATCAT CAAATACAAA AGATGCCTGC TTTGCCTCTG CAGTTGAGTG TTTACAGCAA
    ATCAGTACCA CATTTACACC AGCAGACAAA CTGAAGGTAA TCCAGCAGAC TTTTGAAGAG
    ATATCTCAGA ATGTACTTGA GAGCCTTCAG GAAGATTTTC TGTGGTCTAT GGATGACCTT
    TTCCCTGTTT TTCTCTATGT GGTGCTACGA GCCAGGATAA GGAATTTGGG GTCTGAGGTG
    CACTTAATTG AAGACTTGAT GGATCCTTAC CTGCAACATG GGGAACAAGG CATCATGTTT
    ACCACTTTAA AGGCGTGCTA CTACCAGATT CAACATGAGA AGCTTACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144941.1
    CDS404..5014
    Translation

    Target ORF information:

    RefSeq Version XM_015289455.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289455.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTGGGGGA ACAATTCTTC TGGACAGTGT GCAGTTGCTA ATCAACCATA TGTGCCGGAA 
    CCACAACCTA TCAGTATTTC TGATTCTGAA ACCAGTCCTC TACTATCAGT AAGGGTTTTG
    CAGCTAGCAT GTGGAGAAGA GCACACGTTG GCACTATCAC TAAGCAGAGA GATATGGGCT
    TGGGGGAGTG GCTGCCAGCT AGGTCTTATA ACAACAACTT TCCCAGTGAC AAAGCCACAG
    AAGGTAGAGC ACCTGGCAGG ACGTGTTGTG CTCCAGATTG CTTGTGGAGC CTACCACAGC
    CTGGCACTGG TACAATGTCT CCCTGTGCAG GAGATGAAGC CTATTCCAGA GAGGTGCAAT
    CATTGCAGTC AACTCCTCAT TACCATGACA GACAAGGAAG ATCATGTAAT CATTTCCGAT
    AGTCACTGTT GCCCACTGGG CGTAGCGCTG GCTGATAAAC AACCAGAAAA CCATGCCTTC
    TGTTCTTCGT TGGAAAACCT GGAAGGAAAA AGTGTAACAG GTAGTCAGAG TGAAGACTGT
    AAGAAACCTC TTGTGGAAGA GCATACATTT GTTGCTTTAG AATCTGATGG AGCAAGCTTA
    CTTTCCAGCA ACAATGGACA GGAAGACAGT GGAATTTCTA GCCCTGGAAA TATTCTGTGT
    ACTGACAAAA AAGAAGTGAA AGAATACTTA ATCGGTTTAT CTGATCACAC ATTAAATGAG
    AGCCTGGAGA AAAATATCTG CACAGAATCT GAGCAGCCTT CTCAAGGAGA ACAAATAGGT
    GCTTGTATCC CTGGTCCTCC AGGTACGAGT GCGTCTGCCT TAAACAGCCT GGTGGCCTCC
    TGTGCATCAG CAGTTGGTGT GAGGGTAGCT GCTACATATG AAGCTGGAGC GTTGTCTCTA
    AAGAAAGTAA TGAACTTCTA TGGCACACCT GCAAATGAGC CAGGCCCTCA GCCTGGCAGT
    GGTTCCACAG GGCCTGAAGC TCTGAAAGAC AGTCGAGAAG AGCAGGTCAA ACTGGAATCA
    ATGCAGGGCA AGAAGAGTTC CAGTTTAGTA GATATTAGAG AGGAGGAATC AGAAGGAGGA
    AGTCGAAGAC TTTCCCTGCC TGGCCTGTTA TCACAAGTTT CTCCTAGGCT CTTACGGAAG
    GTAGGACGGG CAAAAATGAG GACTGTGGCT CTGACTCCAA CCTACAGTGG TGAGGCAGAT
    GCTCTTTTAC CATCTTTAAG AACAGAGGTA TGGAGCTGGG GAAGGGGGAA GGATGGACAG
    CTAGGACATG GTGATGTTCT ACCAAGGCTT CAGCCACTGT GTGTGAAAAG TTTAGATGGT
    AAAGAAGTGA TTCAACTCTC TGCTGGTGGT CATCATTCCT TAGCACTCAC TGCCAAATCC
    CAGGTGTATT CATGGGGCAG TAATATCTTT GGTCAGCTTG GTCACACGAA CTCCCCAACA
    ACAGTCCCTC GACTTGCAAA GGTGTGTGGT GATGGTGTTT GGAGTACAGC GGCAGGATCA
    GATTATTCTC TCTTCTTAGC AGATGAGGAA GACTTCCAAC CTGGATTATA TTATTGTGGT
    CAAGAGACAG CAACAGATAA TTTGAGTGAA AATAATTGTA GTAAGAATCC AGTTTTATTA
    CTATCCTGTA GTAAGATAGG ATATATAAGC AATGTGATAG CTGGTGGAGA CAACTGTTTG
    GTCTTGGTAG ACAAAAACAT AATGGGATAT ATTGCCAGTT TGCATGAGTT GGCTTCTACT
    GAGAGAAGGT TTTATTTCAG ACTGAGTGAG ATCAAATCTC AGGTTCTTAG ACCTCTTCTA
    GGTTTAGATA ATTTGGGCAA TGCAAATACA ATCCAGTTGT TACAAGAAAT GGCAAGCAGG
    TTCAGCAAGC TGTGCTATCT CATCGGTCAA CATGGAGCTT CTTTAAGTAG CTTTCTTCAT
    GGAGTTAAAG AAGCCAGGAG TTTGGCCATA CTGAAGCATT CCAGTCTCTT TTTGGATAGT
    TACACTGAAT ACTGTACTTC AATAACAAAT TTCCTGGTAA TGGGAGGATT TATGCTTCTC
    GCAAAGCCTG CAATAGACTT CTTGAGTAAA AGTCAGGAGC TGTTACAGAA ACTGTCTGAG
    TATAATGAGG AAAACCCGCA ACTAGTGGAT GTTCTAAATG CTCTGTTTTT CATGCCATTT
    GGACGACTTC ATGATTATGC CAGAACCTTG CTAAAGCTTG CCACGTGCTT TGAAGTGGCA
    TCTCCAGAAT ACCAGAAGCT ACAAGATTCT AGCTCTTGTT ATGAGAGCCT TGCTGTCCAT
    CTCGGCAGAA AAAGGAAAGA AGCAGAATAC ACACTCAGCT TCTGGAAGAC GTTTCCTGGG
    AAAATGACGG ATTCTTTGCG GAAGCCAGAC CGTCGTTTAA TCTGTGAAAG CAGCAATAGG
    GGGTTGTCCC TACAGCATGC TGGAAGATTC TCTGTGAACT GGTTTATCCT TTTTAACGAT
    GCTCTGGTGC ACGCTCAGTT TTCAACGCAC CATATTTTTG CTTTGTCCAC GCTGTGGGTA
    GAAGCTCTTT CAGAAGAAGC TGGTAATGTG AATGGATTGA AGATTACAAC ACCAGAAGAA
    CAGTTTGTTC TGCTTTCTTC AACACCACAG GAAAAGACGA AGTGGCTGAG GGCAATAAGT
    CAAGCAGTGG ATCAGGCCCT TAAAGGGACT TCTGATTTAA TTCTATATGG AGCTGGTGGG
    AATGTTCCAA GACAGGAGCC TCCTATTTCT CGCAGTGCCA AATACGCCTT CTATAAGGAC
    CCTCGATTTA AAGATGCTGT TTATGATGGG AGATGGCTCT CAGGAAAACC CCATGGCAGA
    GGGATCCTAA AGTGGGCTGA TGGAAGAATG TACTCAGGGA CCTTCCGAAC TGGACTGGAG
    GATGGGTATG GCGAATACAG AGTGCCAAAT AAAGCCTTGA ATAAAGATGA CCACTACGTG
    GGGTACTGGA AGGAAGGCAA AATGTGTGGG CATGGAGTCT ACAGCTATGC TACTGGTGAA
    GTATATGAAG GATGCTTTCA AGATAATATG CGTCACGGCC ATGGCCTGCT GCGCAGTGGG
    AAGCTTACCT CTTCTTCTCC TAGTATGTTC ATTGGACAGT GGACAATGGA TAAGAAGAGT
    GGTTATGGTG TTTTTGATGA TATTACAAGA GGAGAAAAGT ATATGGGAAT GTGGCAAGAT
    GACCTTTGCC AAGGCAATGG TGTTGTGGTT ACTCAGTTTG GATTGTATTA TGAGGGAGCC
    TTCAGCACTA ACAAAATGAT GGGGACTGGC ATTCTGCTTT CTGAGGATGA TACAGTTTAT
    GAAGGGGAAT TTTCAGATAA CTGGACTCTG AGTGGAAAGG GAACACTGAC CTTGCCAAAT
    GGAGATTATA TTGAAGGTCT CTTCAGTGGA GAGTGGGGAT CTGGAATAAA AATCACAGGA
    ACCTACTTCA AACCTAGTTT ATATGAGAGT GAGAAGGATA AGCCTAAAGC ATTGCGGAAA
    CTTGGGATCC TGGCAGTGCC TCCTGATGAG AAATGGAAGG CTGTATTTGA AGAATGTTGG
    AACCAGCTTG GCTGTGAGAG TCCAGGCGAG GGTGATAGAT GGAAGGCTTG GGACAACATT
    GCAGTGGCTC TGACCACCAA CCGACGGCAA CACAAGGACA GTCCAGAATT ACTGAGCCGT
    TCTCATACTC AGACACTGGA AAGTCTAGAA TTTATTCCGC AACACGTTGG TGCCTTCACC
    TTGGAAAAAT ATGAAAATAT CAAAAAATAT TTGATAAAGG CATGTGATAC TCCTCTCCAT
    CCGTTGGGCA AGCTTGTGGA AACACTAGTA GCTGTCTACA GAATGACTTA TGTTGGAGTA
    GGAGCTAATC GACGATTACT GCAGGAGGCA GTAAGAGAGA TCAAGTCCTA CCTCAAACGC
    ATCTTCCAAT TGGTTAGGTT CTTGTTCCCT GATCTTCCGG AAGAGGGCAG CACAATTCCA
    TTTCAGCCAA CGGAAGTAAA GGGAAGGCGT TCATTCTGCA GTGGGAAATC TGACTCCAGA
    TCAGAGTCCC CTGAGCCAGG CTATGTGGTA ACCAGTTTTG GCTTGCTACT CCCTGTACTA
    CTGCCACGTC TCTATCCACC TCTGTTTATG TTATATGCCT TAGACAACGA GCGTGAGGAA
    GATATTTACT GGGAGTGTGT CTTACGTCTA AACAAACAAA CTGATATGGC TCTTCTAAGT
    TTTCTTGGAG TGCAAGCGAA ATTCTGGCCA GGAACTGTAT CCATCCTTGG TGAGAGTAGA
    AAGGTATCAT CAAATACAAA AGATGCCTGC TTTGCCTCTG CAGTTGAGTG TTTACAGCAA
    ATCAGTACCA CATTTACACC AGCAGACAAA CTGAAGGTAA TCCAGCAGAC TTTTGAAGAG
    ATATCTCAGA ATGTACTTGA GAGCCTTCAG GAAGATTTTC TGTGGTCTAT GGATGACCTT
    TTCCCTGTTT TTCTCTATGT GGTGCTACGA GCCAGGATAA GGAATTTGGG GTCTGAGGTG
    CACTTAATTG AAGACTTGAT GGATCCTTAC CTGCAACATG GGGAACAAGG CATCATGTTT
    ACCACTTTAA AGGCGTGCTA CTACCAGATT CAACATGAGA AGCTTACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa38345
    Clone ID Related Accession (Same CDS sequence) XM_015289455.1 , XM_015289455.2
    Accession Version XM_015289455.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product alsin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289455.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTGGGGGA ACAATTCTTC TGGACAGTGT GCAGTTGCTA ATCAACCATA TGTGCCGGAA 
    CCACAACCTA TCAGTATTTC TGATTCTGAA ACCAGTCCTC TACTATCAGT AAGGGTTTTG
    CAGCTAGCAT GTGGAGAAGA GCACACGTTG GCACTATCAC TAAGCAGAGA GATATGGGCT
    TGGGGGAGTG GCTGCCAGCT AGGTCTTATA ACAACAACTT TCCCAGTGAC AAAGCCACAG
    AAGGTAGAGC ACCTGGCAGG ACGTGTTGTG CTCCAGATTG CTTGTGGAGC CTACCACAGC
    CTGGCACTGG TACAATGTCT CCCTGTGCAG GAGATGAAGC CTATTCCAGA GAGGTGCAAT
    CATTGCAGTC AACTCCTCAT TACCATGACA GACAAGGAAG ATCATGTAAT CATTTCCGAT
    AGTCACTGTT GCCCACTGGG CGTAGCGCTG GCTGATAAAC AACCAGAAAA CCATGCCTTC
    TGTTCTTCGT TGGAAAACCT GGAAGGAAAA AGTGTAACAG GTAGTCAGAG TGAAGACTGT
    AAGAAACCTC TTGTGGAAGA GCATACATTT GTTGCTTTAG AATCTGATGG AGCAAGCTTA
    CTTTCCAGCA ACAATGGACA GGAAGACAGT GGAATTTCTA GCCCTGGAAA TATTCTGTGT
    ACTGACAAAA AAGAAGTGAA AGAATACTTA ATCGGTTTAT CTGATCACAC ATTAAATGAG
    AGCCTGGAGA AAAATATCTG CACAGAATCT GAGCAGCCTT CTCAAGGAGA ACAAATAGGT
    GCTTGTATCC CTGGTCCTCC AGGTACGAGT GCGTCTGCCT TAAACAGCCT GGTGGCCTCC
    TGTGCATCAG CAGTTGGTGT GAGGGTAGCT GCTACATATG AAGCTGGAGC GTTGTCTCTA
    AAGAAAGTAA TGAACTTCTA TGGCACACCT GCAAATGAGC CAGGCCCTCA GCCTGGCAGT
    GGTTCCACAG GGCCTGAAGC TCTGAAAGAC AGTCGAGAAG AGCAGGTCAA ACTGGAATCA
    ATGCAGGGCA AGAAGAGTTC CAGTTTAGTA GATATTAGAG AGGAGGAATC AGAAGGAGGA
    AGTCGAAGAC TTTCCCTGCC TGGCCTGTTA TCACAAGTTT CTCCTAGGCT CTTACGGAAG
    GTAGGACGGG CAAAAATGAG GACTGTGGCT CTGACTCCAA CCTACAGTGG TGAGGCAGAT
    GCTCTTTTAC CATCTTTAAG AACAGAGGTA TGGAGCTGGG GAAGGGGGAA GGATGGACAG
    CTAGGACATG GTGATGTTCT ACCAAGGCTT CAGCCACTGT GTGTGAAAAG TTTAGATGGT
    AAAGAAGTGA TTCAACTCTC TGCTGGTGGT CATCATTCCT TAGCACTCAC TGCCAAATCC
    CAGGTGTATT CATGGGGCAG TAATATCTTT GGTCAGCTTG GTCACACGAA CTCCCCAACA
    ACAGTCCCTC GACTTGCAAA GGTGTGTGGT GATGGTGTTT GGAGTACAGC GGCAGGATCA
    GATTATTCTC TCTTCTTAGC AGATGAGGAA GACTTCCAAC CTGGATTATA TTATTGTGGT
    CAAGAGACAG CAACAGATAA TTTGAGTGAA AATAATTGTA GTAAGAATCC AGTTTTATTA
    CTATCCTGTA GTAAGATAGG ATATATAAGC AATGTGATAG CTGGTGGAGA CAACTGTTTG
    GTCTTGGTAG ACAAAAACAT AATGGGATAT ATTGCCAGTT TGCATGAGTT GGCTTCTACT
    GAGAGAAGGT TTTATTTCAG ACTGAGTGAG ATCAAATCTC AGGTTCTTAG ACCTCTTCTA
    GGTTTAGATA ATTTGGGCAA TGCAAATACA ATCCAGTTGT TACAAGAAAT GGCAAGCAGG
    TTCAGCAAGC TGTGCTATCT CATCGGTCAA CATGGAGCTT CTTTAAGTAG CTTTCTTCAT
    GGAGTTAAAG AAGCCAGGAG TTTGGCCATA CTGAAGCATT CCAGTCTCTT TTTGGATAGT
    TACACTGAAT ACTGTACTTC AATAACAAAT TTCCTGGTAA TGGGAGGATT TATGCTTCTC
    GCAAAGCCTG CAATAGACTT CTTGAGTAAA AGTCAGGAGC TGTTACAGAA ACTGTCTGAG
    TATAATGAGG AAAACCCGCA ACTAGTGGAT GTTCTAAATG CTCTGTTTTT CATGCCATTT
    GGACGACTTC ATGATTATGC CAGAACCTTG CTAAAGCTTG CCACGTGCTT TGAAGTGGCA
    TCTCCAGAAT ACCAGAAGCT ACAAGATTCT AGCTCTTGTT ATGAGAGCCT TGCTGTCCAT
    CTCGGCAGAA AAAGGAAAGA AGCAGAATAC ACACTCAGCT TCTGGAAGAC GTTTCCTGGG
    AAAATGACGG ATTCTTTGCG GAAGCCAGAC CGTCGTTTAA TCTGTGAAAG CAGCAATAGG
    GGGTTGTCCC TACAGCATGC TGGAAGATTC TCTGTGAACT GGTTTATCCT TTTTAACGAT
    GCTCTGGTGC ACGCTCAGTT TTCAACGCAC CATATTTTTG CTTTGTCCAC GCTGTGGGTA
    GAAGCTCTTT CAGAAGAAGC TGGTAATGTG AATGGATTGA AGATTACAAC ACCAGAAGAA
    CAGTTTGTTC TGCTTTCTTC AACACCACAG GAAAAGACGA AGTGGCTGAG GGCAATAAGT
    CAAGCAGTGG ATCAGGCCCT TAAAGGGACT TCTGATTTAA TTCTATATGG AGCTGGTGGG
    AATGTTCCAA GACAGGAGCC TCCTATTTCT CGCAGTGCCA AATACGCCTT CTATAAGGAC
    CCTCGATTTA AAGATGCTGT TTATGATGGG AGATGGCTCT CAGGAAAACC CCATGGCAGA
    GGGATCCTAA AGTGGGCTGA TGGAAGAATG TACTCAGGGA CCTTCCGAAC TGGACTGGAG
    GATGGGTATG GCGAATACAG AGTGCCAAAT AAAGCCTTGA ATAAAGATGA CCACTACGTG
    GGGTACTGGA AGGAAGGCAA AATGTGTGGG CATGGAGTCT ACAGCTATGC TACTGGTGAA
    GTATATGAAG GATGCTTTCA AGATAATATG CGTCACGGCC ATGGCCTGCT GCGCAGTGGG
    AAGCTTACCT CTTCTTCTCC TAGTATGTTC ATTGGACAGT GGACAATGGA TAAGAAGAGT
    GGTTATGGTG TTTTTGATGA TATTACAAGA GGAGAAAAGT ATATGGGAAT GTGGCAAGAT
    GACCTTTGCC AAGGCAATGG TGTTGTGGTT ACTCAGTTTG GATTGTATTA TGAGGGAGCC
    TTCAGCACTA ACAAAATGAT GGGGACTGGC ATTCTGCTTT CTGAGGATGA TACAGTTTAT
    GAAGGGGAAT TTTCAGATAA CTGGACTCTG AGTGGAAAGG GAACACTGAC CTTGCCAAAT
    GGAGATTATA TTGAAGGTCT CTTCAGTGGA GAGTGGGGAT CTGGAATAAA AATCACAGGA
    ACCTACTTCA AACCTAGTTT ATATGAGAGT GAGAAGGATA AGCCTAAAGC ATTGCGGAAA
    CTTGGGATCC TGGCAGTGCC TCCTGATGAG AAATGGAAGG CTGTATTTGA AGAATGTTGG
    AACCAGCTTG GCTGTGAGAG TCCAGGCGAG GGTGATAGAT GGAAGGCTTG GGACAACATT
    GCAGTGGCTC TGACCACCAA CCGACGGCAA CACAAGGACA GTCCAGAATT ACTGAGCCGT
    TCTCATACTC AGACACTGGA AAGTCTAGAA TTTATTCCGC AACACGTTGG TGCCTTCACC
    TTGGAAAAAT ATGAAAATAT CAAAAAATAT TTGATAAAGG CATGTGATAC TCCTCTCCAT
    CCGTTGGGCA AGCTTGTGGA AACACTAGTA GCTGTCTACA GAATGACTTA TGTTGGAGTA
    GGAGCTAATC GACGATTACT GCAGGAGGCA GTAAGAGAGA TCAAGTCCTA CCTCAAACGC
    ATCTTCCAAT TGGTTAGGTT CTTGTTCCCT GATCTTCCGG AAGAGGGCAG CACAATTCCA
    TTTCAGCCAA CGGAAGTAAA GGGAAGGCGT TCATTCTGCA GTGGGAAATC TGACTCCAGA
    TCAGAGTCCC CTGAGCCAGG CTATGTGGTA ACCAGTTTTG GCTTGCTACT CCCTGTACTA
    CTGCCACGTC TCTATCCACC TCTGTTTATG TTATATGCCT TAGACAACGA GCGTGAGGAA
    GATATTTACT GGGAGTGTGT CTTACGTCTA AACAAACAAA CTGATATGGC TCTTCTAAGT
    TTTCTTGGAG TGCAAGCGAA ATTCTGGCCA GGAACTGTAT CCATCCTTGG TGAGAGTAGA
    AAGGTATCAT CAAATACAAA AGATGCCTGC TTTGCCTCTG CAGTTGAGTG TTTACAGCAA
    ATCAGTACCA CATTTACACC AGCAGACAAA CTGAAGGTAA TCCAGCAGAC TTTTGAAGAG
    ATATCTCAGA ATGTACTTGA GAGCCTTCAG GAAGATTTTC TGTGGTCTAT GGATGACCTT
    TTCCCTGTTT TTCTCTATGT GGTGCTACGA GCCAGGATAA GGAATTTGGG GTCTGAGGTG
    CACTTAATTG AAGACTTGAT GGATCCTTAC CTGCAACATG GGGAACAAGG CATCATGTTT
    ACCACTTTAA AGGCGTGCTA CTACCAGATT CAACATGAGA AGCTTACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144941.1
    CDS402..5012
    Translation

    Target ORF information:

    RefSeq Version XM_015289455.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289455.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTGGGGGA ACAATTCTTC TGGACAGTGT GCAGTTGCTA ATCAACCATA TGTGCCGGAA 
    CCACAACCTA TCAGTATTTC TGATTCTGAA ACCAGTCCTC TACTATCAGT AAGGGTTTTG
    CAGCTAGCAT GTGGAGAAGA GCACACGTTG GCACTATCAC TAAGCAGAGA GATATGGGCT
    TGGGGGAGTG GCTGCCAGCT AGGTCTTATA ACAACAACTT TCCCAGTGAC AAAGCCACAG
    AAGGTAGAGC ACCTGGCAGG ACGTGTTGTG CTCCAGATTG CTTGTGGAGC CTACCACAGC
    CTGGCACTGG TACAATGTCT CCCTGTGCAG GAGATGAAGC CTATTCCAGA GAGGTGCAAT
    CATTGCAGTC AACTCCTCAT TACCATGACA GACAAGGAAG ATCATGTAAT CATTTCCGAT
    AGTCACTGTT GCCCACTGGG CGTAGCGCTG GCTGATAAAC AACCAGAAAA CCATGCCTTC
    TGTTCTTCGT TGGAAAACCT GGAAGGAAAA AGTGTAACAG GTAGTCAGAG TGAAGACTGT
    AAGAAACCTC TTGTGGAAGA GCATACATTT GTTGCTTTAG AATCTGATGG AGCAAGCTTA
    CTTTCCAGCA ACAATGGACA GGAAGACAGT GGAATTTCTA GCCCTGGAAA TATTCTGTGT
    ACTGACAAAA AAGAAGTGAA AGAATACTTA ATCGGTTTAT CTGATCACAC ATTAAATGAG
    AGCCTGGAGA AAAATATCTG CACAGAATCT GAGCAGCCTT CTCAAGGAGA ACAAATAGGT
    GCTTGTATCC CTGGTCCTCC AGGTACGAGT GCGTCTGCCT TAAACAGCCT GGTGGCCTCC
    TGTGCATCAG CAGTTGGTGT GAGGGTAGCT GCTACATATG AAGCTGGAGC GTTGTCTCTA
    AAGAAAGTAA TGAACTTCTA TGGCACACCT GCAAATGAGC CAGGCCCTCA GCCTGGCAGT
    GGTTCCACAG GGCCTGAAGC TCTGAAAGAC AGTCGAGAAG AGCAGGTCAA ACTGGAATCA
    ATGCAGGGCA AGAAGAGTTC CAGTTTAGTA GATATTAGAG AGGAGGAATC AGAAGGAGGA
    AGTCGAAGAC TTTCCCTGCC TGGCCTGTTA TCACAAGTTT CTCCTAGGCT CTTACGGAAG
    GTAGGACGGG CAAAAATGAG GACTGTGGCT CTGACTCCAA CCTACAGTGG TGAGGCAGAT
    GCTCTTTTAC CATCTTTAAG AACAGAGGTA TGGAGCTGGG GAAGGGGGAA GGATGGACAG
    CTAGGACATG GTGATGTTCT ACCAAGGCTT CAGCCACTGT GTGTGAAAAG TTTAGATGGT
    AAAGAAGTGA TTCAACTCTC TGCTGGTGGT CATCATTCCT TAGCACTCAC TGCCAAATCC
    CAGGTGTATT CATGGGGCAG TAATATCTTT GGTCAGCTTG GTCACACGAA CTCCCCAACA
    ACAGTCCCTC GACTTGCAAA GGTGTGTGGT GATGGTGTTT GGAGTACAGC GGCAGGATCA
    GATTATTCTC TCTTCTTAGC AGATGAGGAA GACTTCCAAC CTGGATTATA TTATTGTGGT
    CAAGAGACAG CAACAGATAA TTTGAGTGAA AATAATTGTA GTAAGAATCC AGTTTTATTA
    CTATCCTGTA GTAAGATAGG ATATATAAGC AATGTGATAG CTGGTGGAGA CAACTGTTTG
    GTCTTGGTAG ACAAAAACAT AATGGGATAT ATTGCCAGTT TGCATGAGTT GGCTTCTACT
    GAGAGAAGGT TTTATTTCAG ACTGAGTGAG ATCAAATCTC AGGTTCTTAG ACCTCTTCTA
    GGTTTAGATA ATTTGGGCAA TGCAAATACA ATCCAGTTGT TACAAGAAAT GGCAAGCAGG
    TTCAGCAAGC TGTGCTATCT CATCGGTCAA CATGGAGCTT CTTTAAGTAG CTTTCTTCAT
    GGAGTTAAAG AAGCCAGGAG TTTGGCCATA CTGAAGCATT CCAGTCTCTT TTTGGATAGT
    TACACTGAAT ACTGTACTTC AATAACAAAT TTCCTGGTAA TGGGAGGATT TATGCTTCTC
    GCAAAGCCTG CAATAGACTT CTTGAGTAAA AGTCAGGAGC TGTTACAGAA ACTGTCTGAG
    TATAATGAGG AAAACCCGCA ACTAGTGGAT GTTCTAAATG CTCTGTTTTT CATGCCATTT
    GGACGACTTC ATGATTATGC CAGAACCTTG CTAAAGCTTG CCACGTGCTT TGAAGTGGCA
    TCTCCAGAAT ACCAGAAGCT ACAAGATTCT AGCTCTTGTT ATGAGAGCCT TGCTGTCCAT
    CTCGGCAGAA AAAGGAAAGA AGCAGAATAC ACACTCAGCT TCTGGAAGAC GTTTCCTGGG
    AAAATGACGG ATTCTTTGCG GAAGCCAGAC CGTCGTTTAA TCTGTGAAAG CAGCAATAGG
    GGGTTGTCCC TACAGCATGC TGGAAGATTC TCTGTGAACT GGTTTATCCT TTTTAACGAT
    GCTCTGGTGC ACGCTCAGTT TTCAACGCAC CATATTTTTG CTTTGTCCAC GCTGTGGGTA
    GAAGCTCTTT CAGAAGAAGC TGGTAATGTG AATGGATTGA AGATTACAAC ACCAGAAGAA
    CAGTTTGTTC TGCTTTCTTC AACACCACAG GAAAAGACGA AGTGGCTGAG GGCAATAAGT
    CAAGCAGTGG ATCAGGCCCT TAAAGGGACT TCTGATTTAA TTCTATATGG AGCTGGTGGG
    AATGTTCCAA GACAGGAGCC TCCTATTTCT CGCAGTGCCA AATACGCCTT CTATAAGGAC
    CCTCGATTTA AAGATGCTGT TTATGATGGG AGATGGCTCT CAGGAAAACC CCATGGCAGA
    GGGATCCTAA AGTGGGCTGA TGGAAGAATG TACTCAGGGA CCTTCCGAAC TGGACTGGAG
    GATGGGTATG GCGAATACAG AGTGCCAAAT AAAGCCTTGA ATAAAGATGA CCACTACGTG
    GGGTACTGGA AGGAAGGCAA AATGTGTGGG CATGGAGTCT ACAGCTATGC TACTGGTGAA
    GTATATGAAG GATGCTTTCA AGATAATATG CGTCACGGCC ATGGCCTGCT GCGCAGTGGG
    AAGCTTACCT CTTCTTCTCC TAGTATGTTC ATTGGACAGT GGACAATGGA TAAGAAGAGT
    GGTTATGGTG TTTTTGATGA TATTACAAGA GGAGAAAAGT ATATGGGAAT GTGGCAAGAT
    GACCTTTGCC AAGGCAATGG TGTTGTGGTT ACTCAGTTTG GATTGTATTA TGAGGGAGCC
    TTCAGCACTA ACAAAATGAT GGGGACTGGC ATTCTGCTTT CTGAGGATGA TACAGTTTAT
    GAAGGGGAAT TTTCAGATAA CTGGACTCTG AGTGGAAAGG GAACACTGAC CTTGCCAAAT
    GGAGATTATA TTGAAGGTCT CTTCAGTGGA GAGTGGGGAT CTGGAATAAA AATCACAGGA
    ACCTACTTCA AACCTAGTTT ATATGAGAGT GAGAAGGATA AGCCTAAAGC ATTGCGGAAA
    CTTGGGATCC TGGCAGTGCC TCCTGATGAG AAATGGAAGG CTGTATTTGA AGAATGTTGG
    AACCAGCTTG GCTGTGAGAG TCCAGGCGAG GGTGATAGAT GGAAGGCTTG GGACAACATT
    GCAGTGGCTC TGACCACCAA CCGACGGCAA CACAAGGACA GTCCAGAATT ACTGAGCCGT
    TCTCATACTC AGACACTGGA AAGTCTAGAA TTTATTCCGC AACACGTTGG TGCCTTCACC
    TTGGAAAAAT ATGAAAATAT CAAAAAATAT TTGATAAAGG CATGTGATAC TCCTCTCCAT
    CCGTTGGGCA AGCTTGTGGA AACACTAGTA GCTGTCTACA GAATGACTTA TGTTGGAGTA
    GGAGCTAATC GACGATTACT GCAGGAGGCA GTAAGAGAGA TCAAGTCCTA CCTCAAACGC
    ATCTTCCAAT TGGTTAGGTT CTTGTTCCCT GATCTTCCGG AAGAGGGCAG CACAATTCCA
    TTTCAGCCAA CGGAAGTAAA GGGAAGGCGT TCATTCTGCA GTGGGAAATC TGACTCCAGA
    TCAGAGTCCC CTGAGCCAGG CTATGTGGTA ACCAGTTTTG GCTTGCTACT CCCTGTACTA
    CTGCCACGTC TCTATCCACC TCTGTTTATG TTATATGCCT TAGACAACGA GCGTGAGGAA
    GATATTTACT GGGAGTGTGT CTTACGTCTA AACAAACAAA CTGATATGGC TCTTCTAAGT
    TTTCTTGGAG TGCAAGCGAA ATTCTGGCCA GGAACTGTAT CCATCCTTGG TGAGAGTAGA
    AAGGTATCAT CAAATACAAA AGATGCCTGC TTTGCCTCTG CAGTTGAGTG TTTACAGCAA
    ATCAGTACCA CATTTACACC AGCAGACAAA CTGAAGGTAA TCCAGCAGAC TTTTGAAGAG
    ATATCTCAGA ATGTACTTGA GAGCCTTCAG GAAGATTTTC TGTGGTCTAT GGATGACCTT
    TTCCCTGTTT TTCTCTATGT GGTGCTACGA GCCAGGATAA GGAATTTGGG GTCTGAGGTG
    CACTTAATTG AAGACTTGAT GGATCCTTAC CTGCAACATG GGGAACAAGG CATCATGTTT
    ACCACTTTAA AGGCGTGCTA CTACCAGATT CAACATGAGA AGCTTACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10920
    Clone ID Related Accession (Same CDS sequence) XM_015289451.1 , XM_015289451.2 , XM_421940.5 , XM_004942617.2 , XM_015289452.1 , XM_004942618.2 , XM_015289454.1 , XM_015289452.2 , XM_015289453.1 , XM_421940.6 , XM_004942617.3 , XM_004942618.3 , XM_015289453.2
    Accession Version XM_015289451.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product alsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144937.1
    CDS456..5405
    Translation

    Target ORF information:

    RefSeq Version XM_015289451.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289451.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10920
    Clone ID Related Accession (Same CDS sequence) XM_015289451.1 , XM_015289451.2 , XM_421940.5 , XM_004942617.2 , XM_015289452.1 , XM_004942618.2 , XM_015289454.1 , XM_015289452.2 , XM_015289453.1 , XM_421940.6 , XM_004942617.3 , XM_004942618.3 , XM_015289453.2
    Accession Version XM_015289451.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product alsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289451.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144937.1
    CDS606..5555
    Translation

    Target ORF information:

    RefSeq Version XM_015289451.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289451.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10920
    Clone ID Related Accession (Same CDS sequence) XM_015289451.1 , XM_015289451.2 , XM_421940.5 , XM_004942617.2 , XM_015289452.1 , XM_004942618.2 , XM_015289454.1 , XM_015289452.2 , XM_015289453.1 , XM_421940.6 , XM_004942617.3 , XM_004942618.3 , XM_015289453.2
    Accession Version XM_421940.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product alsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_421940.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_421940.2
    CDS155..5104
    Translation

    Target ORF information:

    RefSeq Version XM_421940.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_421940.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10920
    Clone ID Related Accession (Same CDS sequence) XM_015289451.1 , XM_015289451.2 , XM_421940.5 , XM_004942617.2 , XM_015289452.1 , XM_004942618.2 , XM_015289454.1 , XM_015289452.2 , XM_015289453.1 , XM_421940.6 , XM_004942617.3 , XM_004942618.3 , XM_015289453.2
    Accession Version XM_004942617.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product alsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004942617.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004942674.1
    CDS151..5100
    Translation

    Target ORF information:

    RefSeq Version XM_004942617.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004942617.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10920
    Clone ID Related Accession (Same CDS sequence) XM_015289451.1 , XM_015289451.2 , XM_421940.5 , XM_004942617.2 , XM_015289452.1 , XM_004942618.2 , XM_015289454.1 , XM_015289452.2 , XM_015289453.1 , XM_421940.6 , XM_004942617.3 , XM_004942618.3 , XM_015289453.2
    Accession Version XM_015289452.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product alsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144938.1
    CDS196..5145
    Translation

    Target ORF information:

    RefSeq Version XM_015289452.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289452.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10920
    Clone ID Related Accession (Same CDS sequence) XM_015289451.1 , XM_015289451.2 , XM_421940.5 , XM_004942617.2 , XM_015289452.1 , XM_004942618.2 , XM_015289454.1 , XM_015289452.2 , XM_015289453.1 , XM_421940.6 , XM_004942617.3 , XM_004942618.3 , XM_015289453.2
    Accession Version XM_004942618.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product alsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004942618.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004942675.1
    CDS145..5094
    Translation

    Target ORF information:

    RefSeq Version XM_004942618.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004942618.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10920
    Clone ID Related Accession (Same CDS sequence) XM_015289451.1 , XM_015289451.2 , XM_421940.5 , XM_004942617.2 , XM_015289452.1 , XM_004942618.2 , XM_015289454.1 , XM_015289452.2 , XM_015289453.1 , XM_421940.6 , XM_004942617.3 , XM_004942618.3 , XM_015289453.2
    Accession Version XM_015289454.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product alsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144940.1
    CDS87..5036
    Translation

    Target ORF information:

    RefSeq Version XM_015289454.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289454.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10920
    Clone ID Related Accession (Same CDS sequence) XM_015289451.1 , XM_015289451.2 , XM_421940.5 , XM_004942617.2 , XM_015289452.1 , XM_004942618.2 , XM_015289454.1 , XM_015289452.2 , XM_015289453.1 , XM_421940.6 , XM_004942617.3 , XM_004942618.3 , XM_015289453.2
    Accession Version XM_015289452.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product alsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289452.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144938.1
    CDS214..5163
    Translation

    Target ORF information:

    RefSeq Version XM_015289452.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289452.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10920
    Clone ID Related Accession (Same CDS sequence) XM_015289451.1 , XM_015289451.2 , XM_421940.5 , XM_004942617.2 , XM_015289452.1 , XM_004942618.2 , XM_015289454.1 , XM_015289452.2 , XM_015289453.1 , XM_421940.6 , XM_004942617.3 , XM_004942618.3 , XM_015289453.2
    Accession Version XM_015289453.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product alsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144939.1
    CDS87..5036
    Translation

    Target ORF information:

    RefSeq Version XM_015289453.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289453.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10920
    Clone ID Related Accession (Same CDS sequence) XM_015289451.1 , XM_015289451.2 , XM_421940.5 , XM_004942617.2 , XM_015289452.1 , XM_004942618.2 , XM_015289454.1 , XM_015289452.2 , XM_015289453.1 , XM_421940.6 , XM_004942617.3 , XM_004942618.3 , XM_015289453.2
    Accession Version XM_421940.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product alsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_421940.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_421940.2
    CDS155..5104
    Translation

    Target ORF information:

    RefSeq Version XM_421940.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_421940.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10920
    Clone ID Related Accession (Same CDS sequence) XM_015289451.1 , XM_015289451.2 , XM_421940.5 , XM_004942617.2 , XM_015289452.1 , XM_004942618.2 , XM_015289454.1 , XM_015289452.2 , XM_015289453.1 , XM_421940.6 , XM_004942617.3 , XM_004942618.3 , XM_015289453.2
    Accession Version XM_004942617.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product alsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004942617.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004942674.1
    CDS96..5045
    Translation

    Target ORF information:

    RefSeq Version XM_004942617.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004942617.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10920
    Clone ID Related Accession (Same CDS sequence) XM_015289451.1 , XM_015289451.2 , XM_421940.5 , XM_004942617.2 , XM_015289452.1 , XM_004942618.2 , XM_015289454.1 , XM_015289452.2 , XM_015289453.1 , XM_421940.6 , XM_004942617.3 , XM_004942618.3 , XM_015289453.2
    Accession Version XM_004942618.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product alsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004942618.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004942675.1
    CDS96..5045
    Translation

    Target ORF information:

    RefSeq Version XM_004942618.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004942618.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10920
    Clone ID Related Accession (Same CDS sequence) XM_015289451.1 , XM_015289451.2 , XM_421940.5 , XM_004942617.2 , XM_015289452.1 , XM_004942618.2 , XM_015289454.1 , XM_015289452.2 , XM_015289453.1 , XM_421940.6 , XM_004942617.3 , XM_004942618.3 , XM_015289453.2
    Accession Version XM_015289453.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product alsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289453.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144939.1
    CDS87..5036
    Translation

    Target ORF information:

    RefSeq Version XM_015289453.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289453.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAC AGGAAAGAAG CTCAGAAGAG GCAGAAGGAG CCAAAGAGAG AGGCCTGGTC 
    TATACCTGGA GGGCTGGTTC CTGCTCTGTA ACTCCAGAAA GGCTTCCAGG CCTCTGTGGA
    AAGACTGTAC TGCAGGCAGC CCTTGGTACA AATCATGGCT TACTTTTAAC AGAAGGTGGT
    GAGGTCTACA GTTTTGGAAA ACTGCCCTGG CGATCAGGAC CAGAGGAGTT CTGTGCTAAT
    AGTCCCATTT TAGAAAATAC CTTGCTTGGG CAGCATGTTA TTACAGTGGC AGCTGGCAGC
    TTCCATAATG GAGCTGTGAC AGAAGGTGGT GTGGTGTACA TGTGGGGGAA CAATTCTTCT
    GGACAGTGTG CAGTTGCTAA TCAACCATAT GTGCCGGAAC CACAACCTAT CAGTATTTCT
    GATTCTGAAA CCAGTCCTCT ACTATCAGTA AGGGTTTTGC AGCTAGCATG TGGAGAAGAG
    CACACGTTGG CACTATCACT AAGCAGAGAG ATATGGGCTT GGGGGAGTGG CTGCCAGCTA
    GGTCTTATAA CAACAACTTT CCCAGTGACA AAGCCACAGA AGGTAGAGCA CCTGGCAGGA
    CGTGTTGTGC TCCAGATTGC TTGTGGAGCC TACCACAGCC TGGCACTGGT ACAATGTCTC
    CCTGTGCAGG AGATGAAGCC TATTCCAGAG AGGTGCAATC ATTGCAGTCA ACTCCTCATT
    ACCATGACAG ACAAGGAAGA TCATGTAATC ATTTCCGATA GTCACTGTTG CCCACTGGGC
    GTAGCGCTGG CTGATAAACA ACCAGAAAAC CATGCCTTCT GTTCTTCGTT GGAAAACCTG
    GAAGGAAAAA GTGTAACAGG TAGTCAGAGT GAAGACTGTA AGAAACCTCT TGTGGAAGAG
    CATACATTTG TTGCTTTAGA ATCTGATGGA GCAAGCTTAC TTTCCAGCAA CAATGGACAG
    GAAGACAGTG GAATTTCTAG CCCTGGAAAT ATTCTGTGTA CTGACAAAAA AGAAGTGAAA
    GAATACTTAA TCGGTTTATC TGATCACACA TTAAATGAGA GCCTGGAGAA AAATATCTGC
    ACAGAATCTG AGCAGCCTTC TCAAGGAGAA CAAATAGGTG CTTGTATCCC TGGTCCTCCA
    GGTACGAGTG CGTCTGCCTT AAACAGCCTG GTGGCCTCCT GTGCATCAGC AGTTGGTGTG
    AGGGTAGCTG CTACATATGA AGCTGGAGCG TTGTCTCTAA AGAAAGTAAT GAACTTCTAT
    GGCACACCTG CAAATGAGCC AGGCCCTCAG CCTGGCAGTG GTTCCACAGG GCCTGAAGCT
    CTGAAAGACA GTCGAGAAGA GCAGGTCAAA CTGGAATCAA TGCAGGGCAA GAAGAGTTCC
    AGTTTAGTAG ATATTAGAGA GGAGGAATCA GAAGGAGGAA GTCGAAGACT TTCCCTGCCT
    GGCCTGTTAT CACAAGTTTC TCCTAGGCTC TTACGGAAGG TAGGACGGGC AAAAATGAGG
    ACTGTGGCTC TGACTCCAAC CTACAGTGGT GAGGCAGATG CTCTTTTACC ATCTTTAAGA
    ACAGAGGTAT GGAGCTGGGG AAGGGGGAAG GATGGACAGC TAGGACATGG TGATGTTCTA
    CCAAGGCTTC AGCCACTGTG TGTGAAAAGT TTAGATGGTA AAGAAGTGAT TCAACTCTCT
    GCTGGTGGTC ATCATTCCTT AGCACTCACT GCCAAATCCC AGGTGTATTC ATGGGGCAGT
    AATATCTTTG GTCAGCTTGG TCACACGAAC TCCCCAACAA CAGTCCCTCG ACTTGCAAAG
    GTGTGTGGTG ATGGTGTTTG GAGTACAGCG GCAGGATCAG ATTATTCTCT CTTCTTAGCA
    GATGAGGAAG ACTTCCAACC TGGATTATAT TATTGTGGTC AAGAGACAGC AACAGATAAT
    TTGAGTGAAA ATAATTGTAG TAAGAATCCA GTTTTATTAC TATCCTGTAG TAAGATAGGA
    TATATAAGCA ATGTGATAGC TGGTGGAGAC AACTGTTTGG TCTTGGTAGA CAAAAACATA
    ATGGGATATA TTGCCAGTTT GCATGAGTTG GCTTCTACTG AGAGAAGGTT TTATTTCAGA
    CTGAGTGAGA TCAAATCTCA GGTTCTTAGA CCTCTTCTAG GTTTAGATAA TTTGGGCAAT
    GCAAATACAA TCCAGTTGTT ACAAGAAATG GCAAGCAGGT TCAGCAAGCT GTGCTATCTC
    ATCGGTCAAC ATGGAGCTTC TTTAAGTAGC TTTCTTCATG GAGTTAAAGA AGCCAGGAGT
    TTGGCCATAC TGAAGCATTC CAGTCTCTTT TTGGATAGTT ACACTGAATA CTGTACTTCA
    ATAACAAATT TCCTGGTAAT GGGAGGATTT ATGCTTCTCG CAAAGCCTGC AATAGACTTC
    TTGAGTAAAA GTCAGGAGCT GTTACAGAAA CTGTCTGAGT ATAATGAGGA AAACCCGCAA
    CTAGTGGATG TTCTAAATGC TCTGTTTTTC ATGCCATTTG GACGACTTCA TGATTATGCC
    AGAACCTTGC TAAAGCTTGC CACGTGCTTT GAAGTGGCAT CTCCAGAATA CCAGAAGCTA
    CAAGATTCTA GCTCTTGTTA TGAGAGCCTT GCTGTCCATC TCGGCAGAAA AAGGAAAGAA
    GCAGAATACA CACTCAGCTT CTGGAAGACG TTTCCTGGGA AAATGACGGA TTCTTTGCGG
    AAGCCAGACC GTCGTTTAAT CTGTGAAAGC AGCAATAGGG GGTTGTCCCT ACAGCATGCT
    GGAAGATTCT CTGTGAACTG GTTTATCCTT TTTAACGATG CTCTGGTGCA CGCTCAGTTT
    TCAACGCACC ATATTTTTGC TTTGTCCACG CTGTGGGTAG AAGCTCTTTC AGAAGAAGCT
    GGTAATGTGA ATGGATTGAA GATTACAACA CCAGAAGAAC AGTTTGTTCT GCTTTCTTCA
    ACACCACAGG AAAAGACGAA GTGGCTGAGG GCAATAAGTC AAGCAGTGGA TCAGGCCCTT
    AAAGGGACTT CTGATTTAAT TCTATATGGA GCTGGTGGGA ATGTTCCAAG ACAGGAGCCT
    CCTATTTCTC GCAGTGCCAA ATACGCCTTC TATAAGGACC CTCGATTTAA AGATGCTGTT
    TATGATGGGA GATGGCTCTC AGGAAAACCC CATGGCAGAG GGATCCTAAA GTGGGCTGAT
    GGAAGAATGT ACTCAGGGAC CTTCCGAACT GGACTGGAGG ATGGGTATGG CGAATACAGA
    GTGCCAAATA AAGCCTTGAA TAAAGATGAC CACTACGTGG GGTACTGGAA GGAAGGCAAA
    ATGTGTGGGC ATGGAGTCTA CAGCTATGCT ACTGGTGAAG TATATGAAGG ATGCTTTCAA
    GATAATATGC GTCACGGCCA TGGCCTGCTG CGCAGTGGGA AGCTTACCTC TTCTTCTCCT
    AGTATGTTCA TTGGACAGTG GACAATGGAT AAGAAGAGTG GTTATGGTGT TTTTGATGAT
    ATTACAAGAG GAGAAAAGTA TATGGGAATG TGGCAAGATG ACCTTTGCCA AGGCAATGGT
    GTTGTGGTTA CTCAGTTTGG ATTGTATTAT GAGGGAGCCT TCAGCACTAA CAAAATGATG
    GGGACTGGCA TTCTGCTTTC TGAGGATGAT ACAGTTTATG AAGGGGAATT TTCAGATAAC
    TGGACTCTGA GTGGAAAGGG AACACTGACC TTGCCAAATG GAGATTATAT TGAAGGTCTC
    TTCAGTGGAG AGTGGGGATC TGGAATAAAA ATCACAGGAA CCTACTTCAA ACCTAGTTTA
    TATGAGAGTG AGAAGGATAA GCCTAAAGCA TTGCGGAAAC TTGGGATCCT GGCAGTGCCT
    CCTGATGAGA AATGGAAGGC TGTATTTGAA GAATGTTGGA ACCAGCTTGG CTGTGAGAGT
    CCAGGCGAGG GTGATAGATG GAAGGCTTGG GACAACATTG CAGTGGCTCT GACCACCAAC
    CGACGGCAAC ACAAGGACAG TCCAGAATTA CTGAGCCGTT CTCATACTCA GACACTGGAA
    AGTCTAGAAT TTATTCCGCA ACACGTTGGT GCCTTCACCT TGGAAAAATA TGAAAATATC
    AAAAAATATT TGATAAAGGC ATGTGATACT CCTCTCCATC CGTTGGGCAA GCTTGTGGAA
    ACACTAGTAG CTGTCTACAG AATGACTTAT GTTGGAGTAG GAGCTAATCG ACGATTACTG
    CAGGAGGCAG TAAGAGAGAT CAAGTCCTAC CTCAAACGCA TCTTCCAATT GGTTAGGTTC
    TTGTTCCCTG ATCTTCCGGA AGAGGGCAGC ACAATTCCAT TTCAGCCAAC GGAAGTAAAG
    GGAAGGCGTT CATTCTGCAG TGGGAAATCT GACTCCAGAT CAGAGTCCCC TGAGCCAGGC
    TATGTGGTAA CCAGTTTTGG CTTGCTACTC CCTGTACTAC TGCCACGTCT CTATCCACCT
    CTGTTTATGT TATATGCCTT AGACAACGAG CGTGAGGAAG ATATTTACTG GGAGTGTGTC
    TTACGTCTAA ACAAACAAAC TGATATGGCT CTTCTAAGTT TTCTTGGAGT GCAAGCGAAA
    TTCTGGCCAG GAACTGTATC CATCCTTGGT GAGAGTAGAA AGGTATCATC AAATACAAAA
    GATGCCTGCT TTGCCTCTGC AGTTGAGTGT TTACAGCAAA TCAGTACCAC ATTTACACCA
    GCAGACAAAC TGAAGGTAAT CCAGCAGACT TTTGAAGAGA TATCTCAGAA TGTACTTGAG
    AGCCTTCAGG AAGATTTTCT GTGGTCTATG GATGACCTTT TCCCTGTTTT TCTCTATGTG
    GTGCTACGAG CCAGGATAAG GAATTTGGGG TCTGAGGTGC ACTTAATTGA AGACTTGATG
    GATCCTTACC TGCAACATGG GGAACAAGGC ATCATGTTTA CCACTTTAAA GGCGTGCTAC
    TACCAGATTC AACATGAGAA GCTTACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.