ALS2 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005202685.4	XP_005202742.1	alsin isoform X1
XM_002685510.5	XP_002685556.1	alsin isoform X1
XM_005199635.3	XP_005199692.1	alsin isoform X1
XM_024981303.1	XP_024837071.1	alsin isoform X3
XM_005199636.3	XP_005199693.1	alsin isoform X2
XM_002685510.6	XP_002685556.1	alsin isoform X1
XM_005202685.3	XP_005202742.1	alsin isoform X1
XM_005202686.3	XP_005202743.1	alsin isoform X2
XM_615861.8	XP_615861.5	alsin isoform X1
XM_005202686.4	XP_005202743.1	alsin isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa227772	XM_024981303.1 Latest version!	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa02940	XM_005199635.3 Latest version!	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa02940	XM_005202685.4 Latest version!	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa02940	XM_615861.8 Latest version!	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa02940	XM_002685510.6 Latest version!	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa02941	XM_005202686.4 Latest version!	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa02941	XM_005199636.3 Latest version!	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OBa02940	XM_002685510.5	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa02940	XM_005202685.3	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa02941	XM_005202686.3	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

ALS2 ( XM_024981303.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837071.1 Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa227772
Clone ID Related Accession (Same CDS sequence)	XM_024981303.1
Accession Version	XM_024981303.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	alsin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGGG AGAACTCTGC TGGCCAGTGT GCGGTAGCTA ACCAGCCATA TGTTCCAGAG 
CCAAACCCTG TCAGCATTTC TGATTCTGAG ACCAGTCCTT TGTTAGCGGT CAGGATCTTG 
CAGTTGGCAT GTGGTGAGGA GCACACTCTG GCATTGTCCC TAAGCAGAGA GATCTGGGCA 
TGGGGTACTG GTTGTCAGTT GGGTCTCATC ACCACCACCT TTCCAGTGAC AAAGCCACAA 
AAGGTGGAAC ACCTTGCTGG GCGAGTAGTG CTCCAAGTTG CTTGCGGTGC CTTCCACAGC 
TTAGCACTGG TCCAGTGCCT CCCTTCCCAG GATCTGAAGC CAGTGCCAGA AAGATGCAAC 
CAATGCAGCC AGCTCTTGAT TACGATGACT GACAAGGAAG ACCACGTGAT TATATCAGAC 
AGCCACTGTT GTCCATTAGG TGTGACACTG TCAGAATCCC AGGCAGAAAT CCAGGCCAGC 
ACCACCGTCA GCCCCTCCTC TGAAACCCTT GACACCCAGG GAGAAGTGTT TGAGAACACT 
CTTGTGCAGA ATGAGCCGCC TGCTGCTACT GAACTGAATG TTGGAAATGT TCAGACCGCA 
AGTGTTCAAA CCACTTCCTT CCCACAAGAT ATCATGGGAA CAACTGAAGT TTCTTCTGCA 
AGTAGACCAT CATACCCTGA CACTCAGGCA GTAAATGAAT ATGTGCAGAA ACTGTCGGAT 
CATTCAGTAA GAGAAAGCTC CGAGAATGGT GAAAAGCCAG TGCCATCTCA GCCTCTTGTA 
GAAGAAGCAA TTCCTAATCT TCACAGCCCA CCAACCACAA GCACTTCAGC CCTCAACAGC 
CTTGTGGTCT CTTGTGCATC TGCTGTTGGT GTGAGAGTGG CTGCTACATA TGAAGCTGGG 
GCCTTGTCTC TTAAGAAAGT TATGAACTTT TATAATACAG CCCCTTGTGA GCCTGGAGCT 
CTGGCAGGCA GCAGTTCCAT AGGCCCAGAA GGTCTGAAAG ATAGCAGAGA AGAACAGGTT 
AAACAGGAGT CAATGCAAGG AAAGAAGAGT TCAAGTCTTG TGGATATCAG AGAAGAAGAG 
TCTGAGGGAG GCAGCCGAAG ACTCTCTCTC CCTGGCTTGT TGTCACAAGT CTCGCCCAGG 
CTCCTGAGGA AAGCTGCACG GGTAAAAACT CGAACAGTGG TTCTGACCCC TACATACAGT 
GGAGAAGCAG ACGCGCTCCT GCCTTCTCTG AGAACAGAGG TGTGGACCTG GGGGAAAGGG 
AAGGAAGGAC AGCTGGGGCA TGGCGATGTT CTGCCCAGGC TTCAACCATT GTGTGTAAAA 
TGTCTGGACG GTAAAGAAGT AATCCATCTG GAGGCAGGTG GTTACCATTC TCTTGCACTT 
ACTGCAAAAT CCCAGGTTTA CTCATGGGGG AGCAACACCT TTGGTCAACT TGGGCATTCA 
GATTTTCCAA CAACAGTTCC CCGTCTTGCA AAGGTGAACA GTGAAAATGG AGTCTGGAGT 
GTGGCTGCAG GCCAGGATTA TTCCCTGTTT TTAGTGGATA CCGAAGACTT CCAGCCTGGG 
TTATGTTACA GTGGCCGGCT GGACCCGACA GAAGGTGACA ACCTTCCAGA GAATCACAGT 
GGTACTAAGA TGCCAGTCCT CCTCTCCTGT AGTAAGCTTG GGTATATAAG CAGAGTGACA 
GCAGGAAAAG ACAGTTATCT AGCCTTGGTG GACAAAAACA TTATGGGATA TATTGCCAGT 
CTCCATGAGT TGGCTACTAC TGAAAGACGC TTCTATTCAA AACTAAGTGA TATCAAGTCC 
CAGATTCTCA GGCCTCTTCT TGGTTTAGAA AATCTGGGCA CTGCAAATAC AGTCCAGCTG 
TTGCAGGAAG TGGCAAGCCG ATTCAGCAAG CTGTGTTACC TAATTGGTCA GCATGGAACC 
TCACTGAGCA GCTTCCTTCA GGGGATAAAG GAAGCCAGGA GTTTGGTCAT CCTGAAGCAT 
GCAAGTCTCT TCTTGGATAG TTATACAGAG TATTGCACAT CTATTACAAA TTTCCTGGTT 
ATGGGAGGAT TCCAGCTTCT TGCTAAACCT GCCATTGATT TCCTAAATAA AAACCAGGAA 
CTGTTGCAAG ATTTATCAGA AGTGGATGAG GAAAACACCC AGTTGATGGA AATTTTGAAT 
ACTCTGTTTT TCTTGCCAAT CAGACGGCTT CATAGTTATG CAAAAGTTTT GCTAAAACTT 
GCTACTTGTT TTGAAGTGAC ATCTCCAGAA TACCAGAAGC TGCAGGATTC CAGCTCTTGT 
TATGAGTCAC TTGCTCTCCA TCTCGGCAGG AAAAGGAAGG AAGCAGAATA CACACTGAGT 
TTCTGGAAGA CCTTTCCTGG AAAAATGACG GATTCCTTGC GGAAGCCCGA GCGTCGGCTG 
CTGTGTGAGA GCAGCAACCG GGCCCTGTCC CTGCAGCACG CTGGGAGGTT TTCTGTGAAC 
TGGTTCATTC TCTTTAATGA TGCCCTAGTC CATGCCCAGT TCTCCACACA CCATGTTTTC 
CCTTTGGCCA CACTGTGGGC TGAGCCACTT TCTGAAGAAG CTGGTGGTGT GAATGGCCTA 
AAGATAACTA CACCCGAGGA GCAGTTCACT CTCATTTCAT CAACACCCCA GGAAAAGACA 
AAGTGGCTCC GGGCTATAAG CCAAGCTGTG GATCAGGCTT TGAGGGGGAT GTCTGATCTT 
CCACCTTATG GAAGTGGCAG CAGTATTCAG AGGCAGGAAC CACCAATTTC ACGCAGTGCC 
AAATACACTT TCTACAAGGA TCCTCGCCTA AAGGACGCAA CCTATGATGG ACGCTGGCTT 
TCAGGGAAGC CTCATGGCAG AGGGGTTTTG AAGTGGCCAG ATGGAAAGAT GTATTCTGGC 
ATGTTCAGGA ATGGCTTGGA AGATGGATAC GGAGAATATA GAATCCCAAA CAAAGCACTG 
AACAAAGAAG ACCATTATGT GGGCCATTGG AAAGAAGGAA AAATGTGTGG TCAAGGAGTC 
TATAGTTATG CCTCTGGTGA AGTGTTTGAA GGCTGTTTTC AAGATAATAT GCGTCATGGT 
CATGGTCTCC TGCGAAGCGG CAAGCTGACT TCCTCTTCTC CCAGTATGTT CATTGGCCAG 
TGGGTAATGG ATAAGAAAGC AGGATATGGT GTCTTTGATG ATATCACGAG AGGGGAGAAG 
TATATGGGAC TGTGGCAAGA TGATGTATGC CAAGGCAATG GTGTAGTAGT TACCCAGTTT 
GGTTTATACT ATGAAGGCAA CTTCCATCTT AATAAAATGA TGGGAAATGG GGTTTTACTT 
TCTGAAGATG ATACTATCTA TGAAGGGGAA TTTTCAGATG ACTGGACCCT CAGTGGAAAG 
GGAACACTGA CTATGCCAAA TGGAGATTAC ATTGAAGGTT ATTTTAGTGG AGAATGGGGA 
TCTGGGATCA AAATCACTGG AACATACTTC AAGCCTAGTC TGTATGAGAG TGATAAAGAC 
CGACCTAAAG TTTTCAGGAA GCTTGGAAAC CTGGCGGTGC CGGCTGATGA GAAGTGGAAA 
GCCGTGTTTG ATGAATGTTG GCATCAGCTC GGCTGTGAGA ACCCAGGCCA AGGGGAAGTT 
TGGAAAGCGT GGGACAATAT TGCTGTGGCC CTGACCACCA ATCGGCGCCA ACACAGAGAC 
AGTCCAGAGA TACTAAGTCG TTCACAGACT CAGACACTAG AGAGTTTGGA ATTCATTCCT 
CAGCATGTTG GTGCCTTCTC CGTGGAGAAA TATGATGACA TTAAGAAATA CTTAATAAAG 
GCCTGCGACA CTCCTCTGCA CCCGCTGGGC AGGCTCGTGG AGACTCTGGT TGCAGTGTAT 
AGAATGACAT ATGTGGGTGT GGGAGCCAAC CGCCGGCTAC TGCAGGAGGC TGTGAAGGAG 
ATTAAGTCCT ACCTTAAGCG AATTTTCCAG CTGGTGAGGT TCTTATTTCC TGAGCTTCCT 
GAAGAGGGCA GCACAATTCC TCTCTCTGCC CCTCTGCCGA CCGAAAGGAA ATCCTTCTGC 
ACTGGAAAGT CAGATTCCAG ATCTGAATCA CCAGAGCCAG GCTATGTGGT AACAGGTTCT 
GGGTTACTGC TTCCTGTACT CCTACCTCGG CTCTACCCAC CACTGTTCAT GCTGTACGCC 
CTGGATAATG ATCGTGAGGA GGACGTTTAC TGGGAATGTG TTCTCCGGCT AAATAAGCAG 
ACAGACCTTG CTCTTTTGGG CTTCCTTGGG GTGCAGAGGA AATTTTGGCC AGTGACCTTG 
TCAGTCCTTG GAGAGAATAA AAAGGTTTTG CCAACCACGA AAGATGCTTG TTTTGCCTCA 
GCTGTGGAAT GTCTGCAACA GATCAGCACA ACGTTTACCC CATCAGACAA ACTAAAAGTC 
ATCCAGCAGA CTTTTGAAGA GATCTCTCAG AGTGTCCTGG CATCACTTCA GGAAGACTTC 
CTATGGTCCA TGGATGACTT ATTCCCTGTT TTTTTATATG TGGTGCTCCG GGCCAGGATT 
AGGAATTTAG GCTCTGAGGT ACACCTCATC GAGGATCTAA TGGACCCATA TCTTCAGCAT 
GGAGAACAGG GTATAATGTT CACTACTTTG AAGGCATGTT ACTATCAGAT CCAGCGTGAG 
AAGCTTAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837071.1
CDS	364..4995
Translation	MWGENSAGQCAVANQPYVPEPNPVSISDSETSPLLAVRILQLACGEEHTLALSLSREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEIQASTTVSPSSETLDTQGEVFENTLVQNEPPAATELNVGNVQTASVQTTSFPQDIMGTTEVSSASRPSYPDTQAVNEYVQKLSDHSVRESSENGEKPVPSQPLVEEAIPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYNTAPCEPGALAGSSSIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGYHSLALTAKSQVYSWGSNTFGQLGHSDFPTTVPRLAKVNSENGVWSVAAGQDYSLFLVDTEDFQPGLCYSGRLDPTEGDNLPENHSGTKMPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPLLGLENLGTANTVQLLQEVASRFSKLCYLIGQHGTSLSSFLQGIKEARSLVILKHASLFLDSYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVDEENTQLMEILNTLFFLPIRRLHSYAKVLLKLATCFEVTSPEYQKLQDSSSCYESLALHLGRKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGMSDLPPYGSGSSIQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGLWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKVFRKLGNLAVPADEKWKAVFDECWHQLGCENPGQGEVWKAWDNIAVALTTNRRQHRDSPEILSRSQTQTLESLEFIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTGSGLLLPVLLPRLYPPLFMLYALDNDREEDVYWECVLRLNKQTDLALLGFLGVQRKFWPVTLSVLGENKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_024981303.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981303.1

ORF Insert Sequence:

ATGTGGGGGG AGAACTCTGC TGGCCAGTGT GCGGTAGCTA ACCAGCCATA TGTTCCAGAG 
CCAAACCCTG TCAGCATTTC TGATTCTGAG ACCAGTCCTT TGTTAGCGGT CAGGATCTTG 
CAGTTGGCAT GTGGTGAGGA GCACACTCTG GCATTGTCCC TAAGCAGAGA GATCTGGGCA 
TGGGGTACTG GTTGTCAGTT GGGTCTCATC ACCACCACCT TTCCAGTGAC AAAGCCACAA 
AAGGTGGAAC ACCTTGCTGG GCGAGTAGTG CTCCAAGTTG CTTGCGGTGC CTTCCACAGC 
TTAGCACTGG TCCAGTGCCT CCCTTCCCAG GATCTGAAGC CAGTGCCAGA AAGATGCAAC 
CAATGCAGCC AGCTCTTGAT TACGATGACT GACAAGGAAG ACCACGTGAT TATATCAGAC 
AGCCACTGTT GTCCATTAGG TGTGACACTG TCAGAATCCC AGGCAGAAAT CCAGGCCAGC 
ACCACCGTCA GCCCCTCCTC TGAAACCCTT GACACCCAGG GAGAAGTGTT TGAGAACACT 
CTTGTGCAGA ATGAGCCGCC TGCTGCTACT GAACTGAATG TTGGAAATGT TCAGACCGCA 
AGTGTTCAAA CCACTTCCTT CCCACAAGAT ATCATGGGAA CAACTGAAGT TTCTTCTGCA 
AGTAGACCAT CATACCCTGA CACTCAGGCA GTAAATGAAT ATGTGCAGAA ACTGTCGGAT 
CATTCAGTAA GAGAAAGCTC CGAGAATGGT GAAAAGCCAG TGCCATCTCA GCCTCTTGTA 
GAAGAAGCAA TTCCTAATCT TCACAGCCCA CCAACCACAA GCACTTCAGC CCTCAACAGC 
CTTGTGGTCT CTTGTGCATC TGCTGTTGGT GTGAGAGTGG CTGCTACATA TGAAGCTGGG 
GCCTTGTCTC TTAAGAAAGT TATGAACTTT TATAATACAG CCCCTTGTGA GCCTGGAGCT 
CTGGCAGGCA GCAGTTCCAT AGGCCCAGAA GGTCTGAAAG ATAGCAGAGA AGAACAGGTT 
AAACAGGAGT CAATGCAAGG AAAGAAGAGT TCAAGTCTTG TGGATATCAG AGAAGAAGAG 
TCTGAGGGAG GCAGCCGAAG ACTCTCTCTC CCTGGCTTGT TGTCACAAGT CTCGCCCAGG 
CTCCTGAGGA AAGCTGCACG GGTAAAAACT CGAACAGTGG TTCTGACCCC TACATACAGT 
GGAGAAGCAG ACGCGCTCCT GCCTTCTCTG AGAACAGAGG TGTGGACCTG GGGGAAAGGG 
AAGGAAGGAC AGCTGGGGCA TGGCGATGTT CTGCCCAGGC TTCAACCATT GTGTGTAAAA 
TGTCTGGACG GTAAAGAAGT AATCCATCTG GAGGCAGGTG GTTACCATTC TCTTGCACTT 
ACTGCAAAAT CCCAGGTTTA CTCATGGGGG AGCAACACCT TTGGTCAACT TGGGCATTCA 
GATTTTCCAA CAACAGTTCC CCGTCTTGCA AAGGTGAACA GTGAAAATGG AGTCTGGAGT 
GTGGCTGCAG GCCAGGATTA TTCCCTGTTT TTAGTGGATA CCGAAGACTT CCAGCCTGGG 
TTATGTTACA GTGGCCGGCT GGACCCGACA GAAGGTGACA ACCTTCCAGA GAATCACAGT 
GGTACTAAGA TGCCAGTCCT CCTCTCCTGT AGTAAGCTTG GGTATATAAG CAGAGTGACA 
GCAGGAAAAG ACAGTTATCT AGCCTTGGTG GACAAAAACA TTATGGGATA TATTGCCAGT 
CTCCATGAGT TGGCTACTAC TGAAAGACGC TTCTATTCAA AACTAAGTGA TATCAAGTCC 
CAGATTCTCA GGCCTCTTCT TGGTTTAGAA AATCTGGGCA CTGCAAATAC AGTCCAGCTG 
TTGCAGGAAG TGGCAAGCCG ATTCAGCAAG CTGTGTTACC TAATTGGTCA GCATGGAACC 
TCACTGAGCA GCTTCCTTCA GGGGATAAAG GAAGCCAGGA GTTTGGTCAT CCTGAAGCAT 
GCAAGTCTCT TCTTGGATAG TTATACAGAG TATTGCACAT CTATTACAAA TTTCCTGGTT 
ATGGGAGGAT TCCAGCTTCT TGCTAAACCT GCCATTGATT TCCTAAATAA AAACCAGGAA 
CTGTTGCAAG ATTTATCAGA AGTGGATGAG GAAAACACCC AGTTGATGGA AATTTTGAAT 
ACTCTGTTTT TCTTGCCAAT CAGACGGCTT CATAGTTATG CAAAAGTTTT GCTAAAACTT 
GCTACTTGTT TTGAAGTGAC ATCTCCAGAA TACCAGAAGC TGCAGGATTC CAGCTCTTGT 
TATGAGTCAC TTGCTCTCCA TCTCGGCAGG AAAAGGAAGG AAGCAGAATA CACACTGAGT 
TTCTGGAAGA CCTTTCCTGG AAAAATGACG GATTCCTTGC GGAAGCCCGA GCGTCGGCTG 
CTGTGTGAGA GCAGCAACCG GGCCCTGTCC CTGCAGCACG CTGGGAGGTT TTCTGTGAAC 
TGGTTCATTC TCTTTAATGA TGCCCTAGTC CATGCCCAGT TCTCCACACA CCATGTTTTC 
CCTTTGGCCA CACTGTGGGC TGAGCCACTT TCTGAAGAAG CTGGTGGTGT GAATGGCCTA 
AAGATAACTA CACCCGAGGA GCAGTTCACT CTCATTTCAT CAACACCCCA GGAAAAGACA 
AAGTGGCTCC GGGCTATAAG CCAAGCTGTG GATCAGGCTT TGAGGGGGAT GTCTGATCTT 
CCACCTTATG GAAGTGGCAG CAGTATTCAG AGGCAGGAAC CACCAATTTC ACGCAGTGCC 
AAATACACTT TCTACAAGGA TCCTCGCCTA AAGGACGCAA CCTATGATGG ACGCTGGCTT 
TCAGGGAAGC CTCATGGCAG AGGGGTTTTG AAGTGGCCAG ATGGAAAGAT GTATTCTGGC 
ATGTTCAGGA ATGGCTTGGA AGATGGATAC GGAGAATATA GAATCCCAAA CAAAGCACTG 
AACAAAGAAG ACCATTATGT GGGCCATTGG AAAGAAGGAA AAATGTGTGG TCAAGGAGTC 
TATAGTTATG CCTCTGGTGA AGTGTTTGAA GGCTGTTTTC AAGATAATAT GCGTCATGGT 
CATGGTCTCC TGCGAAGCGG CAAGCTGACT TCCTCTTCTC CCAGTATGTT CATTGGCCAG 
TGGGTAATGG ATAAGAAAGC AGGATATGGT GTCTTTGATG ATATCACGAG AGGGGAGAAG 
TATATGGGAC TGTGGCAAGA TGATGTATGC CAAGGCAATG GTGTAGTAGT TACCCAGTTT 
GGTTTATACT ATGAAGGCAA CTTCCATCTT AATAAAATGA TGGGAAATGG GGTTTTACTT 
TCTGAAGATG ATACTATCTA TGAAGGGGAA TTTTCAGATG ACTGGACCCT CAGTGGAAAG 
GGAACACTGA CTATGCCAAA TGGAGATTAC ATTGAAGGTT ATTTTAGTGG AGAATGGGGA 
TCTGGGATCA AAATCACTGG AACATACTTC AAGCCTAGTC TGTATGAGAG TGATAAAGAC 
CGACCTAAAG TTTTCAGGAA GCTTGGAAAC CTGGCGGTGC CGGCTGATGA GAAGTGGAAA 
GCCGTGTTTG ATGAATGTTG GCATCAGCTC GGCTGTGAGA ACCCAGGCCA AGGGGAAGTT 
TGGAAAGCGT GGGACAATAT TGCTGTGGCC CTGACCACCA ATCGGCGCCA ACACAGAGAC 
AGTCCAGAGA TACTAAGTCG TTCACAGACT CAGACACTAG AGAGTTTGGA ATTCATTCCT 
CAGCATGTTG GTGCCTTCTC CGTGGAGAAA TATGATGACA TTAAGAAATA CTTAATAAAG 
GCCTGCGACA CTCCTCTGCA CCCGCTGGGC AGGCTCGTGG AGACTCTGGT TGCAGTGTAT 
AGAATGACAT ATGTGGGTGT GGGAGCCAAC CGCCGGCTAC TGCAGGAGGC TGTGAAGGAG 
ATTAAGTCCT ACCTTAAGCG AATTTTCCAG CTGGTGAGGT TCTTATTTCC TGAGCTTCCT 
GAAGAGGGCA GCACAATTCC TCTCTCTGCC CCTCTGCCGA CCGAAAGGAA ATCCTTCTGC 
ACTGGAAAGT CAGATTCCAG ATCTGAATCA CCAGAGCCAG GCTATGTGGT AACAGGTTCT 
GGGTTACTGC TTCCTGTACT CCTACCTCGG CTCTACCCAC CACTGTTCAT GCTGTACGCC 
CTGGATAATG ATCGTGAGGA GGACGTTTAC TGGGAATGTG TTCTCCGGCT AAATAAGCAG 
ACAGACCTTG CTCTTTTGGG CTTCCTTGGG GTGCAGAGGA AATTTTGGCC AGTGACCTTG 
TCAGTCCTTG GAGAGAATAA AAAGGTTTTG CCAACCACGA AAGATGCTTG TTTTGCCTCA 
GCTGTGGAAT GTCTGCAACA GATCAGCACA ACGTTTACCC CATCAGACAA ACTAAAAGTC 
ATCCAGCAGA CTTTTGAAGA GATCTCTCAG AGTGTCCTGG CATCACTTCA GGAAGACTTC 
CTATGGTCCA TGGATGACTT ATTCCCTGTT TTTTTATATG TGGTGCTCCG GGCCAGGATT 
AGGAATTTAG GCTCTGAGGT ACACCTCATC GAGGATCTAA TGGACCCATA TCTTCAGCAT 
GGAGAACAGG GTATAATGTT CACTACTTTG AAGGCATGTT ACTATCAGAT CCAGCGTGAG 
AAGCTTAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_005202685.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202742.1 Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa02940
Clone ID Related Accession (Same CDS sequence)	XM_005202685.4 , XM_002685510.5 , XM_005199635.3 , XM_002685510.6 , XM_005202685.3 , XM_615861.8
Accession Version	XM_005202685.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202685.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTCAGGAAG 
CTTGGAAACC TGGCGGTGCC GGCTGATGAG AAGTGGAAAG CCGTGTTTGA TGAATGTTGG 
CATCAGCTCG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCGTG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAT ACTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCCTTCTCC 
GTGGAGAAAT ATGATGACAT TAAGAAATAC TTAATAAAGG CCTGCGACAC TCCTCTGCAC 
CCGCTGGGCA GGCTCGTGGA GACTCTGGTT GCAGTGTATA GAATGACATA TGTGGGTGTG 
GGAGCCAACC GCCGGCTACT GCAGGAGGCT GTGAAGGAGA TTAAGTCCTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGGTT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTCTCTGCCC CTCTGCCGAC CGAAAGGAAA TCCTTCTGCA CTGGAAAGTC AGATTCCAGA 
TCTGAATCAC CAGAGCCAGG CTATGTGGTA ACAGGTTCTG GGTTACTGCT TCCTGTACTC 
CTACCTCGGC TCTACCCACC ACTGTTCATG CTGTACGCCC TGGATAATGA TCGTGAGGAG 
GACGTTTACT GGGAATGTGT TCTCCGGCTA AATAAGCAGA CAGACCTTGC TCTTTTGGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAGTCCTTGG AGAGAATAAA 
AAGGTTTTGC CAACCACGAA AGATGCTTGT TTTGCCTCAG CTGTGGAATG TCTGCAACAG 
ATCAGCACAA CGTTTACCCC ATCAGACAAA CTAAAAGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TATGGTCCAT GGATGACTTA 
TTCCCTGTTT TTTTATATGT GGTGCTCCGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATCG AGGATCTAAT GGACCCATAT CTTCAGCATG GAGAACAGGG TATAATGTTC 
ACTACTTTGA AGGCATGTTA CTATCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202742.1
CDS	546..5516
Translation	MDSKKKSSTEAEGSKERGLVHVWQAGSCSVTPERLPGWGGKTVLHAALGVKHGVLLTEDGEVYSFGTLPWRSEPAETCPSNPILESALVGQYVVTVATGSFHTGAVTDSGVVYMWGENSAGQCAVANQPYVPEPNPVSISDSETSPLLAVRILQLACGEEHTLALSLSREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEIQASTTVSPSSETLDTQGEVFENTLVQNEPPAATELNVGNVQTASVQTTSFPQDIMGTTEVSSASRPSYPDTQAVNEYVQKLSDHSVRESSENGEKPVPSQPLVEEAIPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYNTAPCEPGALAGSSSIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGYHSLALTAKSQVYSWGSNTFGQLGHSDFPTTVPRLAKVNSENGVWSVAAGQDYSLFLVDTEDFQPGLCYSGRLDPTEGDNLPENHSGTKMPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPLLGLENLGTANTVQLLQEVASRFSKLCYLIGQHGTSLSSFLQGIKEARSLVILKHASLFLDSYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVDEENTQLMEILNTLFFLPIRRLHSYAKVLLKLATCFEVTSPEYQKLQDSSSCYESLALHLGRKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGMSDLPPYGSGSSIQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGLWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKVFRKLGNLAVPADEKWKAVFDECWHQLGCENPGQGEVWKAWDNIAVALTTNRRQHRDSPEILSRSQTQTLESLEFIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTGSGLLLPVLLPRLYPPLFMLYALDNDREEDVYWECVLRLNKQTDLALLGFLGVQRKFWPVTLSVLGENKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_005202685.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202685.4

ORF Insert Sequence:

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTCAGGAAG 
CTTGGAAACC TGGCGGTGCC GGCTGATGAG AAGTGGAAAG CCGTGTTTGA TGAATGTTGG 
CATCAGCTCG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCGTG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAT ACTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCCTTCTCC 
GTGGAGAAAT ATGATGACAT TAAGAAATAC TTAATAAAGG CCTGCGACAC TCCTCTGCAC 
CCGCTGGGCA GGCTCGTGGA GACTCTGGTT GCAGTGTATA GAATGACATA TGTGGGTGTG 
GGAGCCAACC GCCGGCTACT GCAGGAGGCT GTGAAGGAGA TTAAGTCCTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGGTT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTCTCTGCCC CTCTGCCGAC CGAAAGGAAA TCCTTCTGCA CTGGAAAGTC AGATTCCAGA 
TCTGAATCAC CAGAGCCAGG CTATGTGGTA ACAGGTTCTG GGTTACTGCT TCCTGTACTC 
CTACCTCGGC TCTACCCACC ACTGTTCATG CTGTACGCCC TGGATAATGA TCGTGAGGAG 
GACGTTTACT GGGAATGTGT TCTCCGGCTA AATAAGCAGA CAGACCTTGC TCTTTTGGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAGTCCTTGG AGAGAATAAA 
AAGGTTTTGC CAACCACGAA AGATGCTTGT TTTGCCTCAG CTGTGGAATG TCTGCAACAG 
ATCAGCACAA CGTTTACCCC ATCAGACAAA CTAAAAGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TATGGTCCAT GGATGACTTA 
TTCCCTGTTT TTTTATATGT GGTGCTCCGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATCG AGGATCTAAT GGACCCATAT CTTCAGCATG GAGAACAGGG TATAATGTTC 
ACTACTTTGA AGGCATGTTA CTATCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_002685510.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002685556.1 Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X2, mRNA.

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CloneID	OBa02940
Clone ID Related Accession (Same CDS sequence)	XM_005202685.4 , XM_002685510.5 , XM_005199635.3 , XM_002685510.6 , XM_005202685.3 , XM_615861.8
Accession Version	XM_002685510.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103845.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002685510.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTCAGGAAG 
CTTGGAAACC TGGCGGTGCC GGCTGATGAG AAGTGGAAAG CCGTGTTTGA TGAATGTTGG 
CATCAGCTCG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCGTG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAT ACTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCCTTCTCC 
GTGGAGAAAT ATGATGACAT TAAGAAATAC TTAATAAAGG CCTGCGACAC TCCTCTGCAC 
CCGCTGGGCA GGCTCGTGGA GACTCTGGTT GCAGTGTATA GAATGACATA TGTGGGTGTG 
GGAGCCAACC GCCGGCTACT GCAGGAGGCT GTGAAGGAGA TTAAGTCCTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGGTT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTCTCTGCCC CTCTGCCGAC CGAAAGGAAA TCCTTCTGCA CTGGAAAGTC AGATTCCAGA 
TCTGAATCAC CAGAGCCAGG CTATGTGGTA ACAGGTTCTG GGTTACTGCT TCCTGTACTC 
CTACCTCGGC TCTACCCACC ACTGTTCATG CTGTACGCCC TGGATAATGA TCGTGAGGAG 
GACGTTTACT GGGAATGTGT TCTCCGGCTA AATAAGCAGA CAGACCTTGC TCTTTTGGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAGTCCTTGG AGAGAATAAA 
AAGGTTTTGC CAACCACGAA AGATGCTTGT TTTGCCTCAG CTGTGGAATG TCTGCAACAG 
ATCAGCACAA CGTTTACCCC ATCAGACAAA CTAAAAGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TATGGTCCAT GGATGACTTA 
TTCCCTGTTT TTTTATATGT GGTGCTCCGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATCG AGGATCTAAT GGACCCATAT CTTCAGCATG GAGAACAGGG TATAATGTTC 
ACTACTTTGA AGGCATGTTA CTATCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002685556.1
CDS	147..5117
Translation	MDSKKKSSTEAEGSKERGLVHVWQAGSCSVTPERLPGWGGKTVLHAALGVKHGVLLTEDGEVYSFGTLPWRSEPAETCPSNPILESALVGQYVVTVATGSFHTGAVTDSGVVYMWGENSAGQCAVANQPYVPEPNPVSISDSETSPLLAVRILQLACGEEHTLALSLSREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEIQASTTVSPSSETLDTQGEVFENTLVQNEPPAATELNVGNVQTASVQTTSFPQDIMGTTEVSSASRPSYPDTQAVNEYVQKLSDHSVRESSENGEKPVPSQPLVEEAIPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYNTAPCEPGALAGSSSIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGYHSLALTAKSQVYSWGSNTFGQLGHSDFPTTVPRLAKVNSENGVWSVAAGQDYSLFLVDTEDFQPGLCYSGRLDPTEGDNLPENHSGTKMPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPLLGLENLGTANTVQLLQEVASRFSKLCYLIGQHGTSLSSFLQGIKEARSLVILKHASLFLDSYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVDEENTQLMEILNTLFFLPIRRLHSYAKVLLKLATCFEVTSPEYQKLQDSSSCYESLALHLGRKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGMSDLPPYGSGSSIQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGLWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKVFRKLGNLAVPADEKWKAVFDECWHQLGCENPGQGEVWKAWDNIAVALTTNRRQHRDSPEILSRSQTQTLESLEFIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTGSGLLLPVLLPRLYPPLFMLYALDNDREEDVYWECVLRLNKQTDLALLGFLGVQRKFWPVTLSVLGENKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_002685510.5
Organism	Bos taurus(cattle)
Definition	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002685510.5

ORF Insert Sequence:

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTCAGGAAG 
CTTGGAAACC TGGCGGTGCC GGCTGATGAG AAGTGGAAAG CCGTGTTTGA TGAATGTTGG 
CATCAGCTCG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCGTG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAT ACTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCCTTCTCC 
GTGGAGAAAT ATGATGACAT TAAGAAATAC TTAATAAAGG CCTGCGACAC TCCTCTGCAC 
CCGCTGGGCA GGCTCGTGGA GACTCTGGTT GCAGTGTATA GAATGACATA TGTGGGTGTG 
GGAGCCAACC GCCGGCTACT GCAGGAGGCT GTGAAGGAGA TTAAGTCCTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGGTT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTCTCTGCCC CTCTGCCGAC CGAAAGGAAA TCCTTCTGCA CTGGAAAGTC AGATTCCAGA 
TCTGAATCAC CAGAGCCAGG CTATGTGGTA ACAGGTTCTG GGTTACTGCT TCCTGTACTC 
CTACCTCGGC TCTACCCACC ACTGTTCATG CTGTACGCCC TGGATAATGA TCGTGAGGAG 
GACGTTTACT GGGAATGTGT TCTCCGGCTA AATAAGCAGA CAGACCTTGC TCTTTTGGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAGTCCTTGG AGAGAATAAA 
AAGGTTTTGC CAACCACGAA AGATGCTTGT TTTGCCTCAG CTGTGGAATG TCTGCAACAG 
ATCAGCACAA CGTTTACCCC ATCAGACAAA CTAAAAGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TATGGTCCAT GGATGACTTA 
TTCCCTGTTT TTTTATATGT GGTGCTCCGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATCG AGGATCTAAT GGACCCATAT CTTCAGCATG GAGAACAGGG TATAATGTTC 
ACTACTTTGA AGGCATGTTA CTATCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_005199635.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005199692.1 Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X2, mRNA.

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CloneID	OBa02940
Clone ID Related Accession (Same CDS sequence)	XM_005202685.4 , XM_002685510.5 , XM_005199635.3 , XM_002685510.6 , XM_005202685.3 , XM_615861.8
Accession Version	XM_005199635.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199635.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTCAGGAAG 
CTTGGAAACC TGGCGGTGCC GGCTGATGAG AAGTGGAAAG CCGTGTTTGA TGAATGTTGG 
CATCAGCTCG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCGTG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAT ACTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCCTTCTCC 
GTGGAGAAAT ATGATGACAT TAAGAAATAC TTAATAAAGG CCTGCGACAC TCCTCTGCAC 
CCGCTGGGCA GGCTCGTGGA GACTCTGGTT GCAGTGTATA GAATGACATA TGTGGGTGTG 
GGAGCCAACC GCCGGCTACT GCAGGAGGCT GTGAAGGAGA TTAAGTCCTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGGTT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTCTCTGCCC CTCTGCCGAC CGAAAGGAAA TCCTTCTGCA CTGGAAAGTC AGATTCCAGA 
TCTGAATCAC CAGAGCCAGG CTATGTGGTA ACAGGTTCTG GGTTACTGCT TCCTGTACTC 
CTACCTCGGC TCTACCCACC ACTGTTCATG CTGTACGCCC TGGATAATGA TCGTGAGGAG 
GACGTTTACT GGGAATGTGT TCTCCGGCTA AATAAGCAGA CAGACCTTGC TCTTTTGGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAGTCCTTGG AGAGAATAAA 
AAGGTTTTGC CAACCACGAA AGATGCTTGT TTTGCCTCAG CTGTGGAATG TCTGCAACAG 
ATCAGCACAA CGTTTACCCC ATCAGACAAA CTAAAAGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TATGGTCCAT GGATGACTTA 
TTCCCTGTTT TTTTATATGT GGTGCTCCGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATCG AGGATCTAAT GGACCCATAT CTTCAGCATG GAGAACAGGG TATAATGTTC 
ACTACTTTGA AGGCATGTTA CTATCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005199692.1
CDS	147..5117
Translation	MDSKKKSSTEAEGSKERGLVHVWQAGSCSVTPERLPGWGGKTVLHAALGVKHGVLLTEDGEVYSFGTLPWRSEPAETCPSNPILESALVGQYVVTVATGSFHTGAVTDSGVVYMWGENSAGQCAVANQPYVPEPNPVSISDSETSPLLAVRILQLACGEEHTLALSLSREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEIQASTTVSPSSETLDTQGEVFENTLVQNEPPAATELNVGNVQTASVQTTSFPQDIMGTTEVSSASRPSYPDTQAVNEYVQKLSDHSVRESSENGEKPVPSQPLVEEAIPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYNTAPCEPGALAGSSSIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGYHSLALTAKSQVYSWGSNTFGQLGHSDFPTTVPRLAKVNSENGVWSVAAGQDYSLFLVDTEDFQPGLCYSGRLDPTEGDNLPENHSGTKMPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPLLGLENLGTANTVQLLQEVASRFSKLCYLIGQHGTSLSSFLQGIKEARSLVILKHASLFLDSYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVDEENTQLMEILNTLFFLPIRRLHSYAKVLLKLATCFEVTSPEYQKLQDSSSCYESLALHLGRKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGMSDLPPYGSGSSIQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGLWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKVFRKLGNLAVPADEKWKAVFDECWHQLGCENPGQGEVWKAWDNIAVALTTNRRQHRDSPEILSRSQTQTLESLEFIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTGSGLLLPVLLPRLYPPLFMLYALDNDREEDVYWECVLRLNKQTDLALLGFLGVQRKFWPVTLSVLGENKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_005199635.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005199635.3

ORF Insert Sequence:

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTCAGGAAG 
CTTGGAAACC TGGCGGTGCC GGCTGATGAG AAGTGGAAAG CCGTGTTTGA TGAATGTTGG 
CATCAGCTCG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCGTG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAT ACTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCCTTCTCC 
GTGGAGAAAT ATGATGACAT TAAGAAATAC TTAATAAAGG CCTGCGACAC TCCTCTGCAC 
CCGCTGGGCA GGCTCGTGGA GACTCTGGTT GCAGTGTATA GAATGACATA TGTGGGTGTG 
GGAGCCAACC GCCGGCTACT GCAGGAGGCT GTGAAGGAGA TTAAGTCCTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGGTT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTCTCTGCCC CTCTGCCGAC CGAAAGGAAA TCCTTCTGCA CTGGAAAGTC AGATTCCAGA 
TCTGAATCAC CAGAGCCAGG CTATGTGGTA ACAGGTTCTG GGTTACTGCT TCCTGTACTC 
CTACCTCGGC TCTACCCACC ACTGTTCATG CTGTACGCCC TGGATAATGA TCGTGAGGAG 
GACGTTTACT GGGAATGTGT TCTCCGGCTA AATAAGCAGA CAGACCTTGC TCTTTTGGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAGTCCTTGG AGAGAATAAA 
AAGGTTTTGC CAACCACGAA AGATGCTTGT TTTGCCTCAG CTGTGGAATG TCTGCAACAG 
ATCAGCACAA CGTTTACCCC ATCAGACAAA CTAAAAGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TATGGTCCAT GGATGACTTA 
TTCCCTGTTT TTTTATATGT GGTGCTCCGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATCG AGGATCTAAT GGACCCATAT CTTCAGCATG GAGAACAGGG TATAATGTTC 
ACTACTTTGA AGGCATGTTA CTATCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_002685510.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002685556.1 Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X2, mRNA.

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CloneID	OBa02940
Clone ID Related Accession (Same CDS sequence)	XM_005202685.4 , XM_002685510.5 , XM_005199635.3 , XM_002685510.6 , XM_005202685.3 , XM_615861.8
Accession Version	XM_002685510.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002685510.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTCAGGAAG 
CTTGGAAACC TGGCGGTGCC GGCTGATGAG AAGTGGAAAG CCGTGTTTGA TGAATGTTGG 
CATCAGCTCG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCGTG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAT ACTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCCTTCTCC 
GTGGAGAAAT ATGATGACAT TAAGAAATAC TTAATAAAGG CCTGCGACAC TCCTCTGCAC 
CCGCTGGGCA GGCTCGTGGA GACTCTGGTT GCAGTGTATA GAATGACATA TGTGGGTGTG 
GGAGCCAACC GCCGGCTACT GCAGGAGGCT GTGAAGGAGA TTAAGTCCTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGGTT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTCTCTGCCC CTCTGCCGAC CGAAAGGAAA TCCTTCTGCA CTGGAAAGTC AGATTCCAGA 
TCTGAATCAC CAGAGCCAGG CTATGTGGTA ACAGGTTCTG GGTTACTGCT TCCTGTACTC 
CTACCTCGGC TCTACCCACC ACTGTTCATG CTGTACGCCC TGGATAATGA TCGTGAGGAG 
GACGTTTACT GGGAATGTGT TCTCCGGCTA AATAAGCAGA CAGACCTTGC TCTTTTGGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAGTCCTTGG AGAGAATAAA 
AAGGTTTTGC CAACCACGAA AGATGCTTGT TTTGCCTCAG CTGTGGAATG TCTGCAACAG 
ATCAGCACAA CGTTTACCCC ATCAGACAAA CTAAAAGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TATGGTCCAT GGATGACTTA 
TTCCCTGTTT TTTTATATGT GGTGCTCCGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATCG AGGATCTAAT GGACCCATAT CTTCAGCATG GAGAACAGGG TATAATGTTC 
ACTACTTTGA AGGCATGTTA CTATCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002685556.1
CDS	370..5340
Translation	MDSKKKSSTEAEGSKERGLVHVWQAGSCSVTPERLPGWGGKTVLHAALGVKHGVLLTEDGEVYSFGTLPWRSEPAETCPSNPILESALVGQYVVTVATGSFHTGAVTDSGVVYMWGENSAGQCAVANQPYVPEPNPVSISDSETSPLLAVRILQLACGEEHTLALSLSREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEIQASTTVSPSSETLDTQGEVFENTLVQNEPPAATELNVGNVQTASVQTTSFPQDIMGTTEVSSASRPSYPDTQAVNEYVQKLSDHSVRESSENGEKPVPSQPLVEEAIPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYNTAPCEPGALAGSSSIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGYHSLALTAKSQVYSWGSNTFGQLGHSDFPTTVPRLAKVNSENGVWSVAAGQDYSLFLVDTEDFQPGLCYSGRLDPTEGDNLPENHSGTKMPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPLLGLENLGTANTVQLLQEVASRFSKLCYLIGQHGTSLSSFLQGIKEARSLVILKHASLFLDSYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVDEENTQLMEILNTLFFLPIRRLHSYAKVLLKLATCFEVTSPEYQKLQDSSSCYESLALHLGRKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGMSDLPPYGSGSSIQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGLWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKVFRKLGNLAVPADEKWKAVFDECWHQLGCENPGQGEVWKAWDNIAVALTTNRRQHRDSPEILSRSQTQTLESLEFIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTGSGLLLPVLLPRLYPPLFMLYALDNDREEDVYWECVLRLNKQTDLALLGFLGVQRKFWPVTLSVLGENKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_002685510.6
Organism	Bos taurus(cattle)
Definition	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002685510.6

ORF Insert Sequence:

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTCAGGAAG 
CTTGGAAACC TGGCGGTGCC GGCTGATGAG AAGTGGAAAG CCGTGTTTGA TGAATGTTGG 
CATCAGCTCG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCGTG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAT ACTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCCTTCTCC 
GTGGAGAAAT ATGATGACAT TAAGAAATAC TTAATAAAGG CCTGCGACAC TCCTCTGCAC 
CCGCTGGGCA GGCTCGTGGA GACTCTGGTT GCAGTGTATA GAATGACATA TGTGGGTGTG 
GGAGCCAACC GCCGGCTACT GCAGGAGGCT GTGAAGGAGA TTAAGTCCTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGGTT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTCTCTGCCC CTCTGCCGAC CGAAAGGAAA TCCTTCTGCA CTGGAAAGTC AGATTCCAGA 
TCTGAATCAC CAGAGCCAGG CTATGTGGTA ACAGGTTCTG GGTTACTGCT TCCTGTACTC 
CTACCTCGGC TCTACCCACC ACTGTTCATG CTGTACGCCC TGGATAATGA TCGTGAGGAG 
GACGTTTACT GGGAATGTGT TCTCCGGCTA AATAAGCAGA CAGACCTTGC TCTTTTGGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAGTCCTTGG AGAGAATAAA 
AAGGTTTTGC CAACCACGAA AGATGCTTGT TTTGCCTCAG CTGTGGAATG TCTGCAACAG 
ATCAGCACAA CGTTTACCCC ATCAGACAAA CTAAAAGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TATGGTCCAT GGATGACTTA 
TTCCCTGTTT TTTTATATGT GGTGCTCCGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATCG AGGATCTAAT GGACCCATAT CTTCAGCATG GAGAACAGGG TATAATGTTC 
ACTACTTTGA AGGCATGTTA CTATCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_005202685.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202742.1 Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X1, mRNA.

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CloneID	OBa02940
Clone ID Related Accession (Same CDS sequence)	XM_005202685.4 , XM_002685510.5 , XM_005199635.3 , XM_002685510.6 , XM_005202685.3 , XM_615861.8
Accession Version	XM_005202685.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103845.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202685.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTCAGGAAG 
CTTGGAAACC TGGCGGTGCC GGCTGATGAG AAGTGGAAAG CCGTGTTTGA TGAATGTTGG 
CATCAGCTCG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCGTG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAT ACTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCCTTCTCC 
GTGGAGAAAT ATGATGACAT TAAGAAATAC TTAATAAAGG CCTGCGACAC TCCTCTGCAC 
CCGCTGGGCA GGCTCGTGGA GACTCTGGTT GCAGTGTATA GAATGACATA TGTGGGTGTG 
GGAGCCAACC GCCGGCTACT GCAGGAGGCT GTGAAGGAGA TTAAGTCCTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGGTT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTCTCTGCCC CTCTGCCGAC CGAAAGGAAA TCCTTCTGCA CTGGAAAGTC AGATTCCAGA 
TCTGAATCAC CAGAGCCAGG CTATGTGGTA ACAGGTTCTG GGTTACTGCT TCCTGTACTC 
CTACCTCGGC TCTACCCACC ACTGTTCATG CTGTACGCCC TGGATAATGA TCGTGAGGAG 
GACGTTTACT GGGAATGTGT TCTCCGGCTA AATAAGCAGA CAGACCTTGC TCTTTTGGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAGTCCTTGG AGAGAATAAA 
AAGGTTTTGC CAACCACGAA AGATGCTTGT TTTGCCTCAG CTGTGGAATG TCTGCAACAG 
ATCAGCACAA CGTTTACCCC ATCAGACAAA CTAAAAGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TATGGTCCAT GGATGACTTA 
TTCCCTGTTT TTTTATATGT GGTGCTCCGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATCG AGGATCTAAT GGACCCATAT CTTCAGCATG GAGAACAGGG TATAATGTTC 
ACTACTTTGA AGGCATGTTA CTATCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202742.1
CDS	523..5493
Translation	MDSKKKSSTEAEGSKERGLVHVWQAGSCSVTPERLPGWGGKTVLHAALGVKHGVLLTEDGEVYSFGTLPWRSEPAETCPSNPILESALVGQYVVTVATGSFHTGAVTDSGVVYMWGENSAGQCAVANQPYVPEPNPVSISDSETSPLLAVRILQLACGEEHTLALSLSREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEIQASTTVSPSSETLDTQGEVFENTLVQNEPPAATELNVGNVQTASVQTTSFPQDIMGTTEVSSASRPSYPDTQAVNEYVQKLSDHSVRESSENGEKPVPSQPLVEEAIPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYNTAPCEPGALAGSSSIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGYHSLALTAKSQVYSWGSNTFGQLGHSDFPTTVPRLAKVNSENGVWSVAAGQDYSLFLVDTEDFQPGLCYSGRLDPTEGDNLPENHSGTKMPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPLLGLENLGTANTVQLLQEVASRFSKLCYLIGQHGTSLSSFLQGIKEARSLVILKHASLFLDSYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVDEENTQLMEILNTLFFLPIRRLHSYAKVLLKLATCFEVTSPEYQKLQDSSSCYESLALHLGRKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGMSDLPPYGSGSSIQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGLWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKVFRKLGNLAVPADEKWKAVFDECWHQLGCENPGQGEVWKAWDNIAVALTTNRRQHRDSPEILSRSQTQTLESLEFIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTGSGLLLPVLLPRLYPPLFMLYALDNDREEDVYWECVLRLNKQTDLALLGFLGVQRKFWPVTLSVLGENKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_005202685.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202685.3

ORF Insert Sequence:

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTCAGGAAG 
CTTGGAAACC TGGCGGTGCC GGCTGATGAG AAGTGGAAAG CCGTGTTTGA TGAATGTTGG 
CATCAGCTCG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCGTG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAT ACTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCCTTCTCC 
GTGGAGAAAT ATGATGACAT TAAGAAATAC TTAATAAAGG CCTGCGACAC TCCTCTGCAC 
CCGCTGGGCA GGCTCGTGGA GACTCTGGTT GCAGTGTATA GAATGACATA TGTGGGTGTG 
GGAGCCAACC GCCGGCTACT GCAGGAGGCT GTGAAGGAGA TTAAGTCCTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGGTT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTCTCTGCCC CTCTGCCGAC CGAAAGGAAA TCCTTCTGCA CTGGAAAGTC AGATTCCAGA 
TCTGAATCAC CAGAGCCAGG CTATGTGGTA ACAGGTTCTG GGTTACTGCT TCCTGTACTC 
CTACCTCGGC TCTACCCACC ACTGTTCATG CTGTACGCCC TGGATAATGA TCGTGAGGAG 
GACGTTTACT GGGAATGTGT TCTCCGGCTA AATAAGCAGA CAGACCTTGC TCTTTTGGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAGTCCTTGG AGAGAATAAA 
AAGGTTTTGC CAACCACGAA AGATGCTTGT TTTGCCTCAG CTGTGGAATG TCTGCAACAG 
ATCAGCACAA CGTTTACCCC ATCAGACAAA CTAAAAGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TATGGTCCAT GGATGACTTA 
TTCCCTGTTT TTTTATATGT GGTGCTCCGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATCG AGGATCTAAT GGACCCATAT CTTCAGCATG GAGAACAGGG TATAATGTTC 
ACTACTTTGA AGGCATGTTA CTATCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_615861.8 ) cDNA ORF clone, Bos taurus(cattle) -> XP_615861.5 Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X1, mRNA.

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CloneID	OBa02940
Clone ID Related Accession (Same CDS sequence)	XM_005202685.4 , XM_002685510.5 , XM_005199635.3 , XM_002685510.6 , XM_005202685.3 , XM_615861.8
Accession Version	XM_615861.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_615861.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTCAGGAAG 
CTTGGAAACC TGGCGGTGCC GGCTGATGAG AAGTGGAAAG CCGTGTTTGA TGAATGTTGG 
CATCAGCTCG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCGTG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAT ACTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCCTTCTCC 
GTGGAGAAAT ATGATGACAT TAAGAAATAC TTAATAAAGG CCTGCGACAC TCCTCTGCAC 
CCGCTGGGCA GGCTCGTGGA GACTCTGGTT GCAGTGTATA GAATGACATA TGTGGGTGTG 
GGAGCCAACC GCCGGCTACT GCAGGAGGCT GTGAAGGAGA TTAAGTCCTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGGTT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTCTCTGCCC CTCTGCCGAC CGAAAGGAAA TCCTTCTGCA CTGGAAAGTC AGATTCCAGA 
TCTGAATCAC CAGAGCCAGG CTATGTGGTA ACAGGTTCTG GGTTACTGCT TCCTGTACTC 
CTACCTCGGC TCTACCCACC ACTGTTCATG CTGTACGCCC TGGATAATGA TCGTGAGGAG 
GACGTTTACT GGGAATGTGT TCTCCGGCTA AATAAGCAGA CAGACCTTGC TCTTTTGGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAGTCCTTGG AGAGAATAAA 
AAGGTTTTGC CAACCACGAA AGATGCTTGT TTTGCCTCAG CTGTGGAATG TCTGCAACAG 
ATCAGCACAA CGTTTACCCC ATCAGACAAA CTAAAAGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TATGGTCCAT GGATGACTTA 
TTCCCTGTTT TTTTATATGT GGTGCTCCGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATCG AGGATCTAAT GGACCCATAT CTTCAGCATG GAGAACAGGG TATAATGTTC 
ACTACTTTGA AGGCATGTTA CTATCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_615861.5
CDS	523..5493
Translation	MDSKKKSSTEAEGSKERGLVHVWQAGSCSVTPERLPGWGGKTVLHAALGVKHGVLLTEDGEVYSFGTLPWRSEPAETCPSNPILESALVGQYVVTVATGSFHTGAVTDSGVVYMWGENSAGQCAVANQPYVPEPNPVSISDSETSPLLAVRILQLACGEEHTLALSLSREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEIQASTTVSPSSETLDTQGEVFENTLVQNEPPAATELNVGNVQTASVQTTSFPQDIMGTTEVSSASRPSYPDTQAVNEYVQKLSDHSVRESSENGEKPVPSQPLVEEAIPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYNTAPCEPGALAGSSSIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGYHSLALTAKSQVYSWGSNTFGQLGHSDFPTTVPRLAKVNSENGVWSVAAGQDYSLFLVDTEDFQPGLCYSGRLDPTEGDNLPENHSGTKMPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPLLGLENLGTANTVQLLQEVASRFSKLCYLIGQHGTSLSSFLQGIKEARSLVILKHASLFLDSYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVDEENTQLMEILNTLFFLPIRRLHSYAKVLLKLATCFEVTSPEYQKLQDSSSCYESLALHLGRKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGMSDLPPYGSGSSIQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGLWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKVFRKLGNLAVPADEKWKAVFDECWHQLGCENPGQGEVWKAWDNIAVALTTNRRQHRDSPEILSRSQTQTLESLEFIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTGSGLLLPVLLPRLYPPLFMLYALDNDREEDVYWECVLRLNKQTDLALLGFLGVQRKFWPVTLSVLGENKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_615861.8
Organism	Bos taurus(cattle)
Definition	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_615861.8

ORF Insert Sequence:

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTCAGGAAG 
CTTGGAAACC TGGCGGTGCC GGCTGATGAG AAGTGGAAAG CCGTGTTTGA TGAATGTTGG 
CATCAGCTCG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCGTG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAT ACTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCCTTCTCC 
GTGGAGAAAT ATGATGACAT TAAGAAATAC TTAATAAAGG CCTGCGACAC TCCTCTGCAC 
CCGCTGGGCA GGCTCGTGGA GACTCTGGTT GCAGTGTATA GAATGACATA TGTGGGTGTG 
GGAGCCAACC GCCGGCTACT GCAGGAGGCT GTGAAGGAGA TTAAGTCCTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGGTT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTCTCTGCCC CTCTGCCGAC CGAAAGGAAA TCCTTCTGCA CTGGAAAGTC AGATTCCAGA 
TCTGAATCAC CAGAGCCAGG CTATGTGGTA ACAGGTTCTG GGTTACTGCT TCCTGTACTC 
CTACCTCGGC TCTACCCACC ACTGTTCATG CTGTACGCCC TGGATAATGA TCGTGAGGAG 
GACGTTTACT GGGAATGTGT TCTCCGGCTA AATAAGCAGA CAGACCTTGC TCTTTTGGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAGTCCTTGG AGAGAATAAA 
AAGGTTTTGC CAACCACGAA AGATGCTTGT TTTGCCTCAG CTGTGGAATG TCTGCAACAG 
ATCAGCACAA CGTTTACCCC ATCAGACAAA CTAAAAGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TATGGTCCAT GGATGACTTA 
TTCCCTGTTT TTTTATATGT GGTGCTCCGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATCG AGGATCTAAT GGACCCATAT CTTCAGCATG GAGAACAGGG TATAATGTTC 
ACTACTTTGA AGGCATGTTA CTATCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_005199636.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005199693.1 Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa02941
Clone ID Related Accession (Same CDS sequence)	XM_005199636.3 , XM_005202686.3 , XM_005202686.4
Accession Version	XM_005199636.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	alsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199636.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTGAAGCTT 
GGAAACCTGG CGGTGCCGGC TGATGAGAAG TGGAAAGCCG TGTTTGATGA ATGTTGGCAT 
CAGCTCGGCT GTGAGAACCC AGGCCAAGGG GAAGTTTGGA AAGCGTGGGA CAATATTGCT 
GTGGCCCTGA CCACCAATCG GCGCCAACAC AGAGACAGTC CAGAGATACT AAGTCGTTCA 
CAGACTCAGA CACTAGAGAG TTTGGAATTC ATTCCTCAGC ATGTTGGTGC CTTCTCCGTG 
GAGAAATATG ATGACATTAA GAAATACTTA ATAAAGGCCT GCGACACTCC TCTGCACCCG 
CTGGGCAGGC TCGTGGAGAC TCTGGTTGCA GTGTATAGAA TGACATATGT GGGTGTGGGA 
GCCAACCGCC GGCTACTGCA GGAGGCTGTG AAGGAGATTA AGTCCTACCT TAAGCGAATT 
TTCCAGCTGG TGAGGTTCTT ATTTCCTGAG CTTCCTGAAG AGGGCAGCAC AATTCCTCTC 
TCTGCCCCTC TGCCGACCGA AAGGAAATCC TTCTGCACTG GAAAGTCAGA TTCCAGATCT 
GAATCACCAG AGCCAGGCTA TGTGGTAACA GGTTCTGGGT TACTGCTTCC TGTACTCCTA 
CCTCGGCTCT ACCCACCACT GTTCATGCTG TACGCCCTGG ATAATGATCG TGAGGAGGAC 
GTTTACTGGG AATGTGTTCT CCGGCTAAAT AAGCAGACAG ACCTTGCTCT TTTGGGCTTC 
CTTGGGGTGC AGAGGAAATT TTGGCCAGTG ACCTTGTCAG TCCTTGGAGA GAATAAAAAG 
GTTTTGCCAA CCACGAAAGA TGCTTGTTTT GCCTCAGCTG TGGAATGTCT GCAACAGATC 
AGCACAACGT TTACCCCATC AGACAAACTA AAAGTCATCC AGCAGACTTT TGAAGAGATC 
TCTCAGAGTG TCCTGGCATC ACTTCAGGAA GACTTCCTAT GGTCCATGGA TGACTTATTC 
CCTGTTTTTT TATATGTGGT GCTCCGGGCC AGGATTAGGA ATTTAGGCTC TGAGGTACAC 
CTCATCGAGG ATCTAATGGA CCCATATCTT CAGCATGGAG AACAGGGTAT AATGTTCACT 
ACTTTGAAGG CATGTTACTA TCAGATCCAG CGTGAGAAGC TTAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005199693.1
CDS	523..5490
Translation	MDSKKKSSTEAEGSKERGLVHVWQAGSCSVTPERLPGWGGKTVLHAALGVKHGVLLTEDGEVYSFGTLPWRSEPAETCPSNPILESALVGQYVVTVATGSFHTGAVTDSGVVYMWGENSAGQCAVANQPYVPEPNPVSISDSETSPLLAVRILQLACGEEHTLALSLSREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEIQASTTVSPSSETLDTQGEVFENTLVQNEPPAATELNVGNVQTASVQTTSFPQDIMGTTEVSSASRPSYPDTQAVNEYVQKLSDHSVRESSENGEKPVPSQPLVEEAIPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYNTAPCEPGALAGSSSIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGYHSLALTAKSQVYSWGSNTFGQLGHSDFPTTVPRLAKVNSENGVWSVAAGQDYSLFLVDTEDFQPGLCYSGRLDPTEGDNLPENHSGTKMPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPLLGLENLGTANTVQLLQEVASRFSKLCYLIGQHGTSLSSFLQGIKEARSLVILKHASLFLDSYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVDEENTQLMEILNTLFFLPIRRLHSYAKVLLKLATCFEVTSPEYQKLQDSSSCYESLALHLGRKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGMSDLPPYGSGSSIQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGLWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKVLKLGNLAVPADEKWKAVFDECWHQLGCENPGQGEVWKAWDNIAVALTTNRRQHRDSPEILSRSQTQTLESLEFIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTGSGLLLPVLLPRLYPPLFMLYALDNDREEDVYWECVLRLNKQTDLALLGFLGVQRKFWPVTLSVLGENKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_005199636.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005199636.3

ORF Insert Sequence:

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTGAAGCTT 
GGAAACCTGG CGGTGCCGGC TGATGAGAAG TGGAAAGCCG TGTTTGATGA ATGTTGGCAT 
CAGCTCGGCT GTGAGAACCC AGGCCAAGGG GAAGTTTGGA AAGCGTGGGA CAATATTGCT 
GTGGCCCTGA CCACCAATCG GCGCCAACAC AGAGACAGTC CAGAGATACT AAGTCGTTCA 
CAGACTCAGA CACTAGAGAG TTTGGAATTC ATTCCTCAGC ATGTTGGTGC CTTCTCCGTG 
GAGAAATATG ATGACATTAA GAAATACTTA ATAAAGGCCT GCGACACTCC TCTGCACCCG 
CTGGGCAGGC TCGTGGAGAC TCTGGTTGCA GTGTATAGAA TGACATATGT GGGTGTGGGA 
GCCAACCGCC GGCTACTGCA GGAGGCTGTG AAGGAGATTA AGTCCTACCT TAAGCGAATT 
TTCCAGCTGG TGAGGTTCTT ATTTCCTGAG CTTCCTGAAG AGGGCAGCAC AATTCCTCTC 
TCTGCCCCTC TGCCGACCGA AAGGAAATCC TTCTGCACTG GAAAGTCAGA TTCCAGATCT 
GAATCACCAG AGCCAGGCTA TGTGGTAACA GGTTCTGGGT TACTGCTTCC TGTACTCCTA 
CCTCGGCTCT ACCCACCACT GTTCATGCTG TACGCCCTGG ATAATGATCG TGAGGAGGAC 
GTTTACTGGG AATGTGTTCT CCGGCTAAAT AAGCAGACAG ACCTTGCTCT TTTGGGCTTC 
CTTGGGGTGC AGAGGAAATT TTGGCCAGTG ACCTTGTCAG TCCTTGGAGA GAATAAAAAG 
GTTTTGCCAA CCACGAAAGA TGCTTGTTTT GCCTCAGCTG TGGAATGTCT GCAACAGATC 
AGCACAACGT TTACCCCATC AGACAAACTA AAAGTCATCC AGCAGACTTT TGAAGAGATC 
TCTCAGAGTG TCCTGGCATC ACTTCAGGAA GACTTCCTAT GGTCCATGGA TGACTTATTC 
CCTGTTTTTT TATATGTGGT GCTCCGGGCC AGGATTAGGA ATTTAGGCTC TGAGGTACAC 
CTCATCGAGG ATCTAATGGA CCCATATCTT CAGCATGGAG AACAGGGTAT AATGTTCACT 
ACTTTGAAGG CATGTTACTA TCAGATCCAG CGTGAGAAGC TTAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_005202686.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202743.1 Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X3, mRNA.

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CloneID	OBa02941
Clone ID Related Accession (Same CDS sequence)	XM_005199636.3 , XM_005202686.3 , XM_005202686.4
Accession Version	XM_005202686.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	alsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103845.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202686.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTGAAGCTT 
GGAAACCTGG CGGTGCCGGC TGATGAGAAG TGGAAAGCCG TGTTTGATGA ATGTTGGCAT 
CAGCTCGGCT GTGAGAACCC AGGCCAAGGG GAAGTTTGGA AAGCGTGGGA CAATATTGCT 
GTGGCCCTGA CCACCAATCG GCGCCAACAC AGAGACAGTC CAGAGATACT AAGTCGTTCA 
CAGACTCAGA CACTAGAGAG TTTGGAATTC ATTCCTCAGC ATGTTGGTGC CTTCTCCGTG 
GAGAAATATG ATGACATTAA GAAATACTTA ATAAAGGCCT GCGACACTCC TCTGCACCCG 
CTGGGCAGGC TCGTGGAGAC TCTGGTTGCA GTGTATAGAA TGACATATGT GGGTGTGGGA 
GCCAACCGCC GGCTACTGCA GGAGGCTGTG AAGGAGATTA AGTCCTACCT TAAGCGAATT 
TTCCAGCTGG TGAGGTTCTT ATTTCCTGAG CTTCCTGAAG AGGGCAGCAC AATTCCTCTC 
TCTGCCCCTC TGCCGACCGA AAGGAAATCC TTCTGCACTG GAAAGTCAGA TTCCAGATCT 
GAATCACCAG AGCCAGGCTA TGTGGTAACA GGTTCTGGGT TACTGCTTCC TGTACTCCTA 
CCTCGGCTCT ACCCACCACT GTTCATGCTG TACGCCCTGG ATAATGATCG TGAGGAGGAC 
GTTTACTGGG AATGTGTTCT CCGGCTAAAT AAGCAGACAG ACCTTGCTCT TTTGGGCTTC 
CTTGGGGTGC AGAGGAAATT TTGGCCAGTG ACCTTGTCAG TCCTTGGAGA GAATAAAAAG 
GTTTTGCCAA CCACGAAAGA TGCTTGTTTT GCCTCAGCTG TGGAATGTCT GCAACAGATC 
AGCACAACGT TTACCCCATC AGACAAACTA AAAGTCATCC AGCAGACTTT TGAAGAGATC 
TCTCAGAGTG TCCTGGCATC ACTTCAGGAA GACTTCCTAT GGTCCATGGA TGACTTATTC 
CCTGTTTTTT TATATGTGGT GCTCCGGGCC AGGATTAGGA ATTTAGGCTC TGAGGTACAC 
CTCATCGAGG ATCTAATGGA CCCATATCTT CAGCATGGAG AACAGGGTAT AATGTTCACT 
ACTTTGAAGG CATGTTACTA TCAGATCCAG CGTGAGAAGC TTAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202743.1
CDS	523..5490
Translation	MDSKKKSSTEAEGSKERGLVHVWQAGSCSVTPERLPGWGGKTVLHAALGVKHGVLLTEDGEVYSFGTLPWRSEPAETCPSNPILESALVGQYVVTVATGSFHTGAVTDSGVVYMWGENSAGQCAVANQPYVPEPNPVSISDSETSPLLAVRILQLACGEEHTLALSLSREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEIQASTTVSPSSETLDTQGEVFENTLVQNEPPAATELNVGNVQTASVQTTSFPQDIMGTTEVSSASRPSYPDTQAVNEYVQKLSDHSVRESSENGEKPVPSQPLVEEAIPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYNTAPCEPGALAGSSSIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGYHSLALTAKSQVYSWGSNTFGQLGHSDFPTTVPRLAKVNSENGVWSVAAGQDYSLFLVDTEDFQPGLCYSGRLDPTEGDNLPENHSGTKMPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPLLGLENLGTANTVQLLQEVASRFSKLCYLIGQHGTSLSSFLQGIKEARSLVILKHASLFLDSYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVDEENTQLMEILNTLFFLPIRRLHSYAKVLLKLATCFEVTSPEYQKLQDSSSCYESLALHLGRKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGMSDLPPYGSGSSIQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGLWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKVLKLGNLAVPADEKWKAVFDECWHQLGCENPGQGEVWKAWDNIAVALTTNRRQHRDSPEILSRSQTQTLESLEFIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTGSGLLLPVLLPRLYPPLFMLYALDNDREEDVYWECVLRLNKQTDLALLGFLGVQRKFWPVTLSVLGENKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_005202686.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202686.3

ORF Insert Sequence:

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTGAAGCTT 
GGAAACCTGG CGGTGCCGGC TGATGAGAAG TGGAAAGCCG TGTTTGATGA ATGTTGGCAT 
CAGCTCGGCT GTGAGAACCC AGGCCAAGGG GAAGTTTGGA AAGCGTGGGA CAATATTGCT 
GTGGCCCTGA CCACCAATCG GCGCCAACAC AGAGACAGTC CAGAGATACT AAGTCGTTCA 
CAGACTCAGA CACTAGAGAG TTTGGAATTC ATTCCTCAGC ATGTTGGTGC CTTCTCCGTG 
GAGAAATATG ATGACATTAA GAAATACTTA ATAAAGGCCT GCGACACTCC TCTGCACCCG 
CTGGGCAGGC TCGTGGAGAC TCTGGTTGCA GTGTATAGAA TGACATATGT GGGTGTGGGA 
GCCAACCGCC GGCTACTGCA GGAGGCTGTG AAGGAGATTA AGTCCTACCT TAAGCGAATT 
TTCCAGCTGG TGAGGTTCTT ATTTCCTGAG CTTCCTGAAG AGGGCAGCAC AATTCCTCTC 
TCTGCCCCTC TGCCGACCGA AAGGAAATCC TTCTGCACTG GAAAGTCAGA TTCCAGATCT 
GAATCACCAG AGCCAGGCTA TGTGGTAACA GGTTCTGGGT TACTGCTTCC TGTACTCCTA 
CCTCGGCTCT ACCCACCACT GTTCATGCTG TACGCCCTGG ATAATGATCG TGAGGAGGAC 
GTTTACTGGG AATGTGTTCT CCGGCTAAAT AAGCAGACAG ACCTTGCTCT TTTGGGCTTC 
CTTGGGGTGC AGAGGAAATT TTGGCCAGTG ACCTTGTCAG TCCTTGGAGA GAATAAAAAG 
GTTTTGCCAA CCACGAAAGA TGCTTGTTTT GCCTCAGCTG TGGAATGTCT GCAACAGATC 
AGCACAACGT TTACCCCATC AGACAAACTA AAAGTCATCC AGCAGACTTT TGAAGAGATC 
TCTCAGAGTG TCCTGGCATC ACTTCAGGAA GACTTCCTAT GGTCCATGGA TGACTTATTC 
CCTGTTTTTT TATATGTGGT GCTCCGGGCC AGGATTAGGA ATTTAGGCTC TGAGGTACAC 
CTCATCGAGG ATCTAATGGA CCCATATCTT CAGCATGGAG AACAGGGTAT AATGTTCACT 
ACTTTGAAGG CATGTTACTA TCAGATCCAG CGTGAGAAGC TTAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_005202686.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202743.1 Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X3, mRNA.

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CloneID	OBa02941
Clone ID Related Accession (Same CDS sequence)	XM_005199636.3 , XM_005202686.3 , XM_005202686.4
Accession Version	XM_005202686.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	alsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202686.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTGAAGCTT 
GGAAACCTGG CGGTGCCGGC TGATGAGAAG TGGAAAGCCG TGTTTGATGA ATGTTGGCAT 
CAGCTCGGCT GTGAGAACCC AGGCCAAGGG GAAGTTTGGA AAGCGTGGGA CAATATTGCT 
GTGGCCCTGA CCACCAATCG GCGCCAACAC AGAGACAGTC CAGAGATACT AAGTCGTTCA 
CAGACTCAGA CACTAGAGAG TTTGGAATTC ATTCCTCAGC ATGTTGGTGC CTTCTCCGTG 
GAGAAATATG ATGACATTAA GAAATACTTA ATAAAGGCCT GCGACACTCC TCTGCACCCG 
CTGGGCAGGC TCGTGGAGAC TCTGGTTGCA GTGTATAGAA TGACATATGT GGGTGTGGGA 
GCCAACCGCC GGCTACTGCA GGAGGCTGTG AAGGAGATTA AGTCCTACCT TAAGCGAATT 
TTCCAGCTGG TGAGGTTCTT ATTTCCTGAG CTTCCTGAAG AGGGCAGCAC AATTCCTCTC 
TCTGCCCCTC TGCCGACCGA AAGGAAATCC TTCTGCACTG GAAAGTCAGA TTCCAGATCT 
GAATCACCAG AGCCAGGCTA TGTGGTAACA GGTTCTGGGT TACTGCTTCC TGTACTCCTA 
CCTCGGCTCT ACCCACCACT GTTCATGCTG TACGCCCTGG ATAATGATCG TGAGGAGGAC 
GTTTACTGGG AATGTGTTCT CCGGCTAAAT AAGCAGACAG ACCTTGCTCT TTTGGGCTTC 
CTTGGGGTGC AGAGGAAATT TTGGCCAGTG ACCTTGTCAG TCCTTGGAGA GAATAAAAAG 
GTTTTGCCAA CCACGAAAGA TGCTTGTTTT GCCTCAGCTG TGGAATGTCT GCAACAGATC 
AGCACAACGT TTACCCCATC AGACAAACTA AAAGTCATCC AGCAGACTTT TGAAGAGATC 
TCTCAGAGTG TCCTGGCATC ACTTCAGGAA GACTTCCTAT GGTCCATGGA TGACTTATTC 
CCTGTTTTTT TATATGTGGT GCTCCGGGCC AGGATTAGGA ATTTAGGCTC TGAGGTACAC 
CTCATCGAGG ATCTAATGGA CCCATATCTT CAGCATGGAG AACAGGGTAT AATGTTCACT 
ACTTTGAAGG CATGTTACTA TCAGATCCAG CGTGAGAAGC TTAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202743.1
CDS	547..5514
Translation	MDSKKKSSTEAEGSKERGLVHVWQAGSCSVTPERLPGWGGKTVLHAALGVKHGVLLTEDGEVYSFGTLPWRSEPAETCPSNPILESALVGQYVVTVATGSFHTGAVTDSGVVYMWGENSAGQCAVANQPYVPEPNPVSISDSETSPLLAVRILQLACGEEHTLALSLSREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEIQASTTVSPSSETLDTQGEVFENTLVQNEPPAATELNVGNVQTASVQTTSFPQDIMGTTEVSSASRPSYPDTQAVNEYVQKLSDHSVRESSENGEKPVPSQPLVEEAIPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYNTAPCEPGALAGSSSIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGYHSLALTAKSQVYSWGSNTFGQLGHSDFPTTVPRLAKVNSENGVWSVAAGQDYSLFLVDTEDFQPGLCYSGRLDPTEGDNLPENHSGTKMPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPLLGLENLGTANTVQLLQEVASRFSKLCYLIGQHGTSLSSFLQGIKEARSLVILKHASLFLDSYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVDEENTQLMEILNTLFFLPIRRLHSYAKVLLKLATCFEVTSPEYQKLQDSSSCYESLALHLGRKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGMSDLPPYGSGSSIQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGLWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKVLKLGNLAVPADEKWKAVFDECWHQLGCENPGQGEVWKAWDNIAVALTTNRRQHRDSPEILSRSQTQTLESLEFIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTGSGLLLPVLLPRLYPPLFMLYALDNDREEDVYWECVLRLNKQTDLALLGFLGVQRKFWPVTLSVLGENKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_005202686.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202686.4

ORF Insert Sequence:

ATGGACTCAA AGAAGAAAAG TTCCACAGAG GCTGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AGGCAGGGTC CTGTTCTGTG ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCACGCTGC CCTTGGAGTG AAACATGGAG TGCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTCCCCTGG AGAAGTGAGC CAGCAGAGAC TTGTCCAAGC 
AACCCCATTC TGGAGAGTGC CCTGGTTGGG CAGTATGTTG TTACCGTGGC AACAGGGAGT 
TTCCACACCG GAGCAGTCAC AGACAGTGGT GTGGTGTACA TGTGGGGGGA GAACTCTGCT 
GGCCAGTGTG CGGTAGCTAA CCAGCCATAT GTTCCAGAGC CAAACCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTCCTTT GTTAGCGGTC AGGATCTTGC AGTTGGCATG TGGTGAGGAG 
CACACTCTGG CATTGTCCCT AAGCAGAGAG ATCTGGGCAT GGGGTACTGG TTGTCAGTTG 
GGTCTCATCA CCACCACCTT TCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGG 
CGAGTAGTGC TCCAAGTTGC TTGCGGTGCC TTCCACAGCT TAGCACTGGT CCAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTGCCAGAA AGATGCAACC AATGCAGCCA GCTCTTGATT 
ACGATGACTG ACAAGGAAGA CCACGTGATT ATATCAGACA GCCACTGTTG TCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAATC CAGGCCAGCA CCACCGTCAG CCCCTCCTCT 
GAAACCCTTG ACACCCAGGG AGAAGTGTTT GAGAACACTC TTGTGCAGAA TGAGCCGCCT 
GCTGCTACTG AACTGAATGT TGGAAATGTT CAGACCGCAA GTGTTCAAAC CACTTCCTTC 
CCACAAGATA TCATGGGAAC AACTGAAGTT TCTTCTGCAA GTAGACCATC ATACCCTGAC 
ACTCAGGCAG TAAATGAATA TGTGCAGAAA CTGTCGGATC ATTCAGTAAG AGAAAGCTCC 
GAGAATGGTG AAAAGCCAGT GCCATCTCAG CCTCTTGTAG AAGAAGCAAT TCCTAATCTT 
CACAGCCCAC CAACCACAAG CACTTCAGCC CTCAACAGCC TTGTGGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAATACAGC CCCTTGTGAG CCTGGAGCTC TGGCAGGCAG CAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAGTC AATGCAAGGA 
AAGAAGAGTT CAAGTCTTGT GGATATCAGA GAAGAAGAGT CTGAGGGAGG CAGCCGAAGA 
CTCTCTCTCC CTGGCTTGTT GTCACAAGTC TCGCCCAGGC TCCTGAGGAA AGCTGCACGG 
GTAAAAACTC GAACAGTGGT TCTGACCCCT ACATACAGTG GAGAAGCAGA CGCGCTCCTG 
CCTTCTCTGA GAACAGAGGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGGCAT 
GGCGATGTTC TGCCCAGGCT TCAACCATTG TGTGTAAAAT GTCTGGACGG TAAAGAAGTA 
ATCCATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCAAAATC CCAGGTTTAC 
TCATGGGGGA GCAACACCTT TGGTCAACTT GGGCATTCAG ATTTTCCAAC AACAGTTCCC 
CGTCTTGCAA AGGTGAACAG TGAAAATGGA GTCTGGAGTG TGGCTGCAGG CCAGGATTAT 
TCCCTGTTTT TAGTGGATAC CGAAGACTTC CAGCCTGGGT TATGTTACAG TGGCCGGCTG 
GACCCGACAG AAGGTGACAA CCTTCCAGAG AATCACAGTG GTACTAAGAT GCCAGTCCTC 
CTCTCCTGTA GTAAGCTTGG GTATATAAGC AGAGTGACAG CAGGAAAAGA CAGTTATCTA 
GCCTTGGTGG ACAAAAACAT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACTACT 
GAAAGACGCT TCTATTCAAA ACTAAGTGAT ATCAAGTCCC AGATTCTCAG GCCTCTTCTT 
GGTTTAGAAA ATCTGGGCAC TGCAAATACA GTCCAGCTGT TGCAGGAAGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAACCT CACTGAGCAG CTTCCTTCAG 
GGGATAAAGG AAGCCAGGAG TTTGGTCATC CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAGCTTCTT 
GCTAAACCTG CCATTGATTT CCTAAATAAA AACCAGGAAC TGTTGCAAGA TTTATCAGAA 
GTGGATGAGG AAAACACCCA GTTGATGGAA ATTTTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGGCTTC ATAGTTATGC AAAAGTTTTG CTAAAACTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAGCT GCAGGATTCC AGCTCTTGTT ATGAGTCACT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCAGAATAC ACACTGAGTT TCTGGAAGAC CTTTCCTGGA 
AAAATGACGG ATTCCTTGCG GAAGCCCGAG CGTCGGCTGC TGTGTGAGAG CAGCAACCGG 
GCCCTGTCCC TGCAGCACGC TGGGAGGTTT TCTGTGAACT GGTTCATTCT CTTTAATGAT 
GCCCTAGTCC ATGCCCAGTT CTCCACACAC CATGTTTTCC CTTTGGCCAC ACTGTGGGCT 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCCTAA AGATAACTAC ACCCGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACAA AGTGGCTCCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGATG TCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCAATTTCA CGCAGTGCCA AATACACTTT CTACAAGGAT 
CCTCGCCTAA AGGACGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AGTGGCCAGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATACG GAGAATATAG AATCCCAAAC AAAGCACTGA ACAAAGAAGA CCATTATGTG 
GGCCATTGGA AAGAAGGAAA AATGTGTGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAATATG CGTCATGGTC ATGGTCTCCT GCGAAGCGGC 
AAGCTGACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACGAGA GGGGAGAAGT ATATGGGACT GTGGCAAGAT 
GATGTATGCC AAGGCAATGG TGTAGTAGTT ACCCAGTTTG GTTTATACTA TGAAGGCAAC 
TTCCATCTTA ATAAAATGAT GGGAAATGGG GTTTTACTTT CTGAAGATGA TACTATCTAT 
GAAGGGGAAT TTTCAGATGA CTGGACCCTC AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGGATCAA AATCACTGGA 
ACATACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACC GACCTAAAGT TTTGAAGCTT 
GGAAACCTGG CGGTGCCGGC TGATGAGAAG TGGAAAGCCG TGTTTGATGA ATGTTGGCAT 
CAGCTCGGCT GTGAGAACCC AGGCCAAGGG GAAGTTTGGA AAGCGTGGGA CAATATTGCT 
GTGGCCCTGA CCACCAATCG GCGCCAACAC AGAGACAGTC CAGAGATACT AAGTCGTTCA 
CAGACTCAGA CACTAGAGAG TTTGGAATTC ATTCCTCAGC ATGTTGGTGC CTTCTCCGTG 
GAGAAATATG ATGACATTAA GAAATACTTA ATAAAGGCCT GCGACACTCC TCTGCACCCG 
CTGGGCAGGC TCGTGGAGAC TCTGGTTGCA GTGTATAGAA TGACATATGT GGGTGTGGGA 
GCCAACCGCC GGCTACTGCA GGAGGCTGTG AAGGAGATTA AGTCCTACCT TAAGCGAATT 
TTCCAGCTGG TGAGGTTCTT ATTTCCTGAG CTTCCTGAAG AGGGCAGCAC AATTCCTCTC 
TCTGCCCCTC TGCCGACCGA AAGGAAATCC TTCTGCACTG GAAAGTCAGA TTCCAGATCT 
GAATCACCAG AGCCAGGCTA TGTGGTAACA GGTTCTGGGT TACTGCTTCC TGTACTCCTA 
CCTCGGCTCT ACCCACCACT GTTCATGCTG TACGCCCTGG ATAATGATCG TGAGGAGGAC 
GTTTACTGGG AATGTGTTCT CCGGCTAAAT AAGCAGACAG ACCTTGCTCT TTTGGGCTTC 
CTTGGGGTGC AGAGGAAATT TTGGCCAGTG ACCTTGTCAG TCCTTGGAGA GAATAAAAAG 
GTTTTGCCAA CCACGAAAGA TGCTTGTTTT GCCTCAGCTG TGGAATGTCT GCAACAGATC 
AGCACAACGT TTACCCCATC AGACAAACTA AAAGTCATCC AGCAGACTTT TGAAGAGATC 
TCTCAGAGTG TCCTGGCATC ACTTCAGGAA GACTTCCTAT GGTCCATGGA TGACTTATTC 
CCTGTTTTTT TATATGTGGT GCTCCGGGCC AGGATTAGGA ATTTAGGCTC TGAGGTACAC 
CTCATCGAGG ATCTAATGGA CCCATATCTT CAGCATGGAG AACAGGGTAT AATGTTCACT 
ACTTTGAAGG CATGTTACTA TCAGATCCAG CGTGAGAAGC TTAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ALS2
Entrez Gene ID	535750
Full Name	ALS2, alsin Rho guanine nucleotide exchange factor
General protein information	Preferred Names ALS2, alsin Rho guanine nucleotide exchange factor Names alsin amyotrophic lateral sclerosis 2 (juvenile)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Canis lupus familiaris (dog)	ALS2	XP_003640234.1
Danio rerio (zebrafish)	als2b	XP_002662342.2
Homo sapiens (human)	ALS2	NP_065970.2
Bos taurus (cattle)	ALS2	XP_005202742.1
Rattus norvegicus (Norway rat)	Als2	NP_001013431.1
Pan troglodytes (chimpanzee)	ALS2	NP_001073389.1
Xenopus tropicalis (tropical clawed frog)	als2	XP_004917813.1
	ALS2	XP_001091562.2
Mus musculus (house mouse)	Als2	NP_001153420.2
Gallus gallus (chicken)	ALS2	XP_421940.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ALS2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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