Als2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Als2
Entrez Gene ID	363235
Full Name	ALS2, alsin Rho guanine nucleotide exchange factor
General protein information	Preferred Names ALS2, alsin Rho guanine nucleotide exchange factor Names alsin alsin Rho guanine nucleotide exchange factor amyotrophic lateral sclerosis 2 (juvenile) amyotrophic lateral sclerosis 2 protein homolog amyotrophic lateral sclerosis protein 2 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q31

mRNA and Protein(s)

mRNA	Protein	Name
XM_017596517.1	XP_017452006.1	alsin isoform X2
XM_006245001.3	XP_006245063.1	alsin isoform X1
XM_008767118.2	XP_008765340.1	alsin isoform X3
NM_001013413.1	NP_001013431.1	alsin

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ALS2	XP_003640234.1
Danio rerio (zebrafish)	als2b	XP_002662342.2
Homo sapiens (human)	ALS2	NP_065970.2
Bos taurus (cattle)	ALS2	XP_005202742.1
Rattus norvegicus (Norway rat)	Als2	NP_001013431.1
Pan troglodytes (chimpanzee)	ALS2	NP_001073389.1
Xenopus tropicalis (tropical clawed frog)	als2	XP_004917813.1
	ALS2	XP_001091562.2
Mus musculus (house mouse)	Als2	NP_001153420.2
Gallus gallus (chicken)	ALS2	XP_421940.2

Gene Ontology
Bibliography

Related articles in PubMed

Molecular interaction of neurocalcin alpha with alsin (ALS2).
Masutani T, Taguchi K, Kumanogoh H, Nakamura S, Maekawa S
Neuroscience letters438(1)26-8(2008 Jun)

ALS2CL, a novel ALS2-interactor, modulates ALS2-mediated endosome dynamics.
Suzuki-Utsunomiya K, Hadano S, Otomo A, Kunita R, Mizumura H, Osuga H, Ikeda JE
Biochemical and biophysical research communications354(2)491-7(2007 Mar)

Amyotrophic lateral sclerosis 2-deficiency leads to neuronal degeneration in amyotrophic lateral sclerosis through altered AMPA receptor trafficking.
Lai C, Xie C, McCormack SG, Chiang HC, Michalak MK, Lin X, Chandran J, Shim H, Shimoji M, Cookson MR, Huganir RL, Rothstein JD, Price DL, Wong PC, Martin LJ, Zhu JJ, Cai H
The Journal of neuroscience : the official journal of the Society for Neuroscience26(45)11798-806(2006 Nov)

Alsin/Rac1 signaling controls survival and growth of spinal motoneurons.
Jacquier A, Buhler E, Sch?fer MK, Bohl D, Blanchard S, Beclin C, Haase G
Annals of neurology60(1)105-17(2006 Jul)

Als2-deficient mice exhibit disturbances in endosome trafficking associated with motor behavioral abnormalities.
Devon RS, Orban PC, Gerrow K, Barbieri MA, Schwab C, Cao LP, Helm JR, Bissada N, Cruz-Aguado R, Davidson TL, Witmer J, Metzler M, Lam CK, Tetzlaff W, Simpson EM, McCaffery JM, El-Husseini AE, Leavitt BR, Hayden MR
Proceedings of the National Academy of Sciences of the United States of America103(25)9595-600(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that alsin binds cell membrane in a Ca(2+)-dependent manner through NCalpha and regulates membrane dynamics.
Title: Molecular interaction of neurocalcin alpha with alsin (ALS2).

ALS2/Alsin has a role in regulating Rac-PAK signaling and neurite outgrowth
Title: ALS2/Alsin regulates Rac-PAK signaling and neurite outgrowth.

Als2 gene encodes a large protein termed alsin, which contains a number of predicted cell signaling and protein trafficking sequence motifs.
Title: Alsin is a Rab5 and Rac1 guanine nucleotide exchange factor.

The following Als2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Als2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa48506	XM_008767118.2 Latest version!	Rattus norvegicus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa62808	XM_017596517.1 Latest version!	Rattus norvegicus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa03946	NM_001013413.1 Latest version!	Rattus norvegicus alsin Rho guanine nucleotide exchange factor ALS2 (Als2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa03946	XM_006245001.3 Latest version!	Rattus norvegicus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Als2 ( XM_008767118.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765340.1 Rattus norvegicus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa48506
Clone ID Related Accession (Same CDS sequence)	XM_008767118.2
Accession Version	XM_008767118.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alsin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767118.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAAAAG CTCAGCAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGAGGA 
AAAACAGTTC TTCAGGCGGC CCTTGGCGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC CCTTCCCTGG AAAAGTGAAG CAGCAGAAAT TTGTCCAAGC 
AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTG TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCGGTGAC AGAAAGTGGA GTGGTGTACA TGTGGGGGGA GAACGCTGCC 
GGGCAGTGTG CAGTAGCGAA CCAGCAGTAT GTGTCGGAAC CAAGTCCTGT CAGCATTTCT 
GACTCGGAGA CCAGCCCCTT GCTAGCAGTT AGGATTCTGC AGTTGGCATG TGGCGAGGAA 
CACACACTGG CATTGTCAAT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG 
GGTCTCATCA CCACCACCTT CCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGA 
CGAGTGGTGC TCCAGGTGGC CTGTGGTGCG TTCCACAGCC TTGCACTTGT GCAGTGCCTC 
CCTCCCCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC 
ACCATGACAG ACAAAGAGGA CCACGTGATA ATATCAGACA GCCATTGCTG CCCTTTAGGT 
GTGACACTGT CTGAATCTCA AGCAGAAAAG CACGCCAGCA CTGTGACCAG CCCTCACCCG 
GAGACGCTGG ATGGGCAAGG AGAGGTGTTT GAGAACACAG TAGCAGAAGC TGAACTGAAT 
ATGGGAAGCG ATCAGACCAC AAGTGGCAGT GCCATTTCCG CCCAGCAGAA CATCGTGGGA 
ATGGCTGAAG TGTCTTCTGC CAGAACAGCT CCCTCATACC CAGACACCCA GACGGTAACT 
GCATACCTGC AGAAGCTATC AGAGCATTCA GTGAAGGAGA ACCATGAGCG TGAGGAAAAG 
CTACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC 
ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTGAGA 
GTGGCCGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTATAGC 
ACTGCCCCCT GCGAGCCAGG AGCTCCGTCA GGCACTGCCT CCACAGGCCC AGAAAGTCTG 
AAAGATCTCA GAGAAGAGCA GGTGAAACAG GAATCACTAC AAGGGAAGAA AAGCTCAAGC 
CTCATGGATA TCAGAGAGGA GGAGTCGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGA 
TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCCG CCAGGGTGAA AACACGGACA 
GTGGTTCTGA CTCCCACGTA CAGTGGAGAA GCAGATGCCC TCCTGCCTTC GCTGAGAACA 
GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTGG GGCACGGCGA TGTCCTGCCC 
AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTCATCCA CTTGGAGGCA 
GGTGGCTCCC ACTCTCTCGC ACTGACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT 
ACCTTCGGTC AGCTGGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCGAAGGTT 
AGCAGTGAAA GTGGAGTCTG GAGTGTAGCT GCAGGCCACG GTTATTCCCT GTTTTTAGTG 
GACACTGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GCCAGGACCG TGCAGAAGGT 
GACACCCTGC CAGAGAATCC CAGTGGTACA ACGACTCCGG TACTTCTCTC CTGTAGTAAG 
GATTTTCTCC GTGTGACTCT GACTGGCCCC TCAGGAACTC GCTCTGTAGA CCAGCCTGGC 
CTCAAACTCA CAGAGATCTG CCTGCCTCTG CCTCCAGAGT GCTGGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765340.1
CDS	448..2538
Translation	MDSKKKSSAEAEGSKERGLVHVWQAGSFSLTPERLPGWGGKTVLQAALGVKHGVLLTEDGEVYSFGTLPWKSEAAEICPSSPLLESALVGHHVVTVATGSFHSGAVTESGVVYMWGENAAGQCAVANQQYVSEPSPVSISDSETSPLLAVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPPQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEKHASTVTSPHPETLDGQGEVFENTVAEAELNMGSDQTTSGSAISAQQNIVGMAEVSSARTAPSYPDTQTVTAYLQKLSEHSVKENHEREEKLPQVQPLVEEAVPDLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSTAPCEPGAPSGTASTGPESLKDLREEQVKQESLQGKKSSSLMDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGSHSLALTAKSQVYSWGSNTFGQLGHSEFPTTVPRLSKVSSESGVWSVAAGHGYSLFLVDTEDFQPGLYYSGRQDRAEGDTLPENPSGTTTPVLLSCSKDFLRVTLTGPSGTRSVDQPGLKLTEICLPLPPECWD

Target ORF information:

RefSeq Version	XM_008767118.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767118.2

ORF Insert Sequence:

ATGGACTCAA AGAAGAAAAG CTCAGCAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGAGGA 
AAAACAGTTC TTCAGGCGGC CCTTGGCGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC CCTTCCCTGG AAAAGTGAAG CAGCAGAAAT TTGTCCAAGC 
AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTG TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCGGTGAC AGAAAGTGGA GTGGTGTACA TGTGGGGGGA GAACGCTGCC 
GGGCAGTGTG CAGTAGCGAA CCAGCAGTAT GTGTCGGAAC CAAGTCCTGT CAGCATTTCT 
GACTCGGAGA CCAGCCCCTT GCTAGCAGTT AGGATTCTGC AGTTGGCATG TGGCGAGGAA 
CACACACTGG CATTGTCAAT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG 
GGTCTCATCA CCACCACCTT CCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGA 
CGAGTGGTGC TCCAGGTGGC CTGTGGTGCG TTCCACAGCC TTGCACTTGT GCAGTGCCTC 
CCTCCCCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC 
ACCATGACAG ACAAAGAGGA CCACGTGATA ATATCAGACA GCCATTGCTG CCCTTTAGGT 
GTGACACTGT CTGAATCTCA AGCAGAAAAG CACGCCAGCA CTGTGACCAG CCCTCACCCG 
GAGACGCTGG ATGGGCAAGG AGAGGTGTTT GAGAACACAG TAGCAGAAGC TGAACTGAAT 
ATGGGAAGCG ATCAGACCAC AAGTGGCAGT GCCATTTCCG CCCAGCAGAA CATCGTGGGA 
ATGGCTGAAG TGTCTTCTGC CAGAACAGCT CCCTCATACC CAGACACCCA GACGGTAACT 
GCATACCTGC AGAAGCTATC AGAGCATTCA GTGAAGGAGA ACCATGAGCG TGAGGAAAAG 
CTACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC 
ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTGAGA 
GTGGCCGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTATAGC 
ACTGCCCCCT GCGAGCCAGG AGCTCCGTCA GGCACTGCCT CCACAGGCCC AGAAAGTCTG 
AAAGATCTCA GAGAAGAGCA GGTGAAACAG GAATCACTAC AAGGGAAGAA AAGCTCAAGC 
CTCATGGATA TCAGAGAGGA GGAGTCGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGA 
TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCCG CCAGGGTGAA AACACGGACA 
GTGGTTCTGA CTCCCACGTA CAGTGGAGAA GCAGATGCCC TCCTGCCTTC GCTGAGAACA 
GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTGG GGCACGGCGA TGTCCTGCCC 
AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTCATCCA CTTGGAGGCA 
GGTGGCTCCC ACTCTCTCGC ACTGACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT 
ACCTTCGGTC AGCTGGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCGAAGGTT 
AGCAGTGAAA GTGGAGTCTG GAGTGTAGCT GCAGGCCACG GTTATTCCCT GTTTTTAGTG 
GACACTGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GCCAGGACCG TGCAGAAGGT 
GACACCCTGC CAGAGAATCC CAGTGGTACA ACGACTCCGG TACTTCTCTC CTGTAGTAAG 
GATTTTCTCC GTGTGACTCT GACTGGCCCC TCAGGAACTC GCTCTGTAGA CCAGCCTGGC 
CTCAAACTCA CAGAGATCTG CCTGCCTCTG CCTCCAGAGT GCTGGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Als2 ( XM_017596517.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452006.1 Rattus norvegicus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa62808
Clone ID Related Accession (Same CDS sequence)	XM_017596517.1
Accession Version	XM_017596517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAAAAG CTCAGCAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGAGGA 
AAAACAGTTC TTCAGGCGGC CCTTGGCGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC CCTTCCCTGG AAAAGTGAAG CAGCAGAAAT TTGTCCAAGC 
AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTG TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCGGTGAC AGAAAGTGGA GTGGTGTACA TGTGGGGGGA GAACGCTGCC 
GGGCAGTGTG CAGTAGCGAA CCAGCAGTAT GTGTCGGAAC CAAGTCCTGT CAGCATTTCT 
GACTCGGAGA CCAGCCCCTT GCTAGCAGTT AGGATTCTGC AGTTGGCATG TGGCGAGGAA 
CACACACTGG CATTGTCAAT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG 
GGTCTCATCA CCACCACCTT CCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGA 
CGAGTGGTGC TCCAGGTGGC CTGTGGTGCG TTCCACAGCC TTGCACTTGT GCAGTGCCTC 
CCTCCCCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC 
ACCATGACAG ACAAAGAGGA CCACGTGATA ATATCAGACA GCCATTGCTG CCCTTTAGGT 
GTGACACTGT CTGAATCTCA AGCAGAAAAG CACGCCAGCA CTGTGACCAG CCCTCACCCG 
GAGACGCTGG ATGGGCAAGG AGAGGTGTTT GAGAACACAG TAGCAGAAGC TGAACTGAAT 
ATGGGAAGCG ATCAGACCAC AAGTGGCAGT GCCATTTCCG CCCAGCAGAA CATCGTGGGA 
ATGGCTGAAG TGTCTTCTGC CAGAACAGCT CCCTCATACC CAGACACCCA GACGGTAACT 
GCATACCTGC AGAAGCTATC AGAGCATTCA GTGAAGGAGA ACCATGAGCG TGAGGAAAAG 
CTACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC 
ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTGAGA 
GTGGCCGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTATAGC 
ACTGCCCCCT GCGAGCCAGG AGCTCCGTCA GGCACTGCCT CCACAGGCCC AGAAAGTCTG 
AAAGATCTCA GAGAAGAGCA GGTGAAACAG GAATCACTAC AAGGGAAGAA AAGCTCAAGC 
CTCATGGATA TCAGAGAGGA GGAGTCGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGA 
TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCCG CCAGGGTGAA AACACGGACA 
GTGGTTCTGA CTCCCACGTA CAGTGGAGAA GCAGATGCCC TCCTGCCTTC GCTGAGAACA 
GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTGG GGCACGGCGA TGTCCTGCCC 
AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTCATCCA CTTGGAGGCA 
GGTGGCTCCC ACTCTCTCGC ACTGACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT 
ACCTTCGGTC AGCTGGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCGAAGGTT 
AGCAGTGAAA GTGGAGTCTG GAGTGTAGCT GCAGGCCACG GTTATTCCCT GTTTTTAGTG 
GACACTGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GCCAGGACCG TGCAGAAGGT 
GACACCCTGC CAGAGAATCC CAGTGGTACA ACGACTCCGC TTGGATATAT CAGCAGAGTG 
ACAGCAGGAA AAGATAGCTA CCTAGCCTTG GTGGATAAGA ACATCATGGG ATACATCGCC 
AGCCTCCATG AGTTGGCTAC CACAGAAAGA CGGTTTTACT CGAAACTAAG TGAAATCAAG 
TCACAGATAC TTAGGCCTCT TCTCAGTCTG GAACATTTGG GCACTGTGAC CACTGTCCAG 
CTGTTGCAGG AAGTCGCCAG CCGGTTCAGC AAGTTGTGTT ACCTCATTGG GCAGCATGGA 
GCCTCACTGA GCAGCTACCT GCAAGGCATG AAGGAAGCCA GGAACCTGGT CATCATGAAG 
CACTCGAGTC TTTTCTTGGA CAGTTACACA GAGTATTGTA CATCAGTTTC AAATTTTCTG 
GTTATGGGAG GATCCCAGCT TCTTGCTAAG CCTGCAATTG ATTTCCTAAA TAAAAACCAA 
GAACTCTTGC AAGATTTGTC AGAAGTGAAT GATGAGAACA CTCAGTTGAT GGAAATACTG 
AATGCGCTGT TTTTCTTGCC AATCAGACGA CTTCATAATT ATGCAAAAGT TTTGCTAAAG 
CTCGCCACTT GCTTTGAAGT GACATCACCA GAGTATCAAA AGCTGCAGGA TTCCAGTTCT 
TGTTATGAGT CTCTTGCTCT CCATCTTGGC AAGAAGAGGA AGGAAGCAGA GTACACGCTG 
AGCTTCTGGA AGACCTTCCC TGGAAAAATG ACGGATTCCT TGAGGAAGCC AGAGCGCCGG 
CTGCTGTGTG AGAGCAGTAA CCGAGCCCTC TCCCTGCAGC ATGCTGGCAG GTTTTCTGTG 
AATTGGTTCA TTCTCTTCAA TGATGCCCTG GTCCATGCTC AGTTCTCCAC ACACCACGTG 
TTCCCTTTGG CCACACTCTG GGCAGAGCCA CTTTCTGAAG AAACTGGTGG CGTGAATGGC 
TTAAAGATAA CCACACCTGA AGAACAGTTC ACGCTCATTT CTTCAACACC CCAGGAAAAG 
ACCAAGTGGC TTCGGGCTAT CAGCCAAGCC GTGGACCAGG CTTTGAGGGG GACGTCTGAC 
TTCCCACTTT ACGGAGGCAG CAGCACTGTT CAGAGGCAGG AACCACCCAT CTCAAGAAGT 
GCCAAATACA CTTTCTACAA GGATACTCGC CTAAAGGACG CCACTTACGA TGGGCGCTGG 
CTTTCAGGGA AGCCGCACGG AAGGGGTGTT TTGAAGTGGC CTGATGGGAA GGTGTATTCT 
GGCACGTTCA GGAATGGCTT GGAAGACGGA TATGGTGAAT ACAGAATCCC AAACAAGGCC 
CTGAACAAAG AAGACCATTA CGTGGGCCAT TGGAAGGAGG GGAAAATGTG TGGGCAAGGA 
GTGTACAGCT ATGCCTCTGG TGAAGTGTTT GAAGGCTGCT TTCAAGATAA CATGCGCCAC 
GGGCACGGTC TCCTCCGCAG TGGAAAACTG ACTTCTTCCT CTCCCAGCAT GTTCATTGGC 
CAGTGGGTAA TGGACAAGAA AGCAGGGTAT GGCGTCTTCG ATGATATCAC CAGGGGAGAA 
AAGTACATGG GAATGTGGCA GGACGATGCA TGCCAAGGGA ATGGGGTGGT GGTCACCCAG 
TTTGGGTTAT ACTACGAAGG CAACTTCCAC CTTAATAAAA TGATGGGAAA TGGGGTTTTG 
CTTTCGGAAG ATGATACTAT ATATGAAGGA GAATTTTCTG ATGACTGGAC ACTTTGTGGA 
AAGGGAACAC TGACTATGCC AAATGGAGAT TATATTGAAG GTTATTTTAG TGGAGAATGG 
GGATCTGGGA TAAAAATCAC TGGGACCTAC TTCAAACCTA GCCTATATGA GAGTGATAAG 
GACAGGCCCA AAGCCTTCAG GAAGCTGGGA AACCTGGCCG TGGCCGCCGA TGAGAAATGG 
AGAGCAGTGT TTGACGAATG CTGGCGCCAG CTGGGCTGTG AGAGCCCAGG CCAAGGGGAG 
GTTTGGAAAG CGTGGGATAA TATTGCTGTG GCCTTGACCA CCAACCGTCG CCAGCATAAA 
GACAGTCCAG AAATACTAAG CCGTTCACAG ACTCAGACCC TGGAGAGTTT GGAGTACATT 
CCCCAGCACG TTGGCGCCTT CTCTGTGGAG AAATATGATG ATATCAAGAA GTATTTAATA 
AAGGCCTGTG ACACTCCTCT GCACCCACTG GGCAGGCTTG TGGAGACCCT GGTTGCAGTG 
TATAGAATGA CTTATGTGGG CGTAGGGGCC AACCGTCGGT TACTGCAGGA AGCTGTGAAG 
GAGATTAAGT CTTATCTCAA GAGGATTTTC CAACTTGTGA GGTTCTTGTT TCCTGAGCTT 
CCTGAAGAGG GCAGCACAGT TCCTCTCTCT GCTCCTCTGC CCACTGGAAG AAGATCCTTC 
TGCACTGGGA AATCAGATTC CAGATCCGAG TCACCAGAGC CAGGTTATGT AGTAACAAGT 
TCTGGCTTAC TGCTTCCGGT GCTGCTGCCT CGGCTCTACC CGCCTCTCTT CATGCTCTAT 
GCCCTGGACA ATGACCGAGA GGAAGACATT TACTGGGAAT GTGTGCTTCG ACTAAACAAG 
CAGCCAGATA TTGCTCTCCT GGGCTTTCTT GGAGTACAGA GGAAATTCTG GCCAGCAACC 
TTGTCAATCC TTGGAGAGAG TAAAAAGGTA CTGCCATCCA CAAAGGATGC TTGTTTTGCA 
TCTGCAGTAG AATGTCTGCA GCAGATCAGC ACAACATTTA CTCCATCAGA CAAACTTAAA 
GTGATCCAGC AGACCTTCGA AGAAATCTCT CAGAGTGTGC TGGCGTCGCT GCAGGAGGAC 
TTCCTTTGGT CCATGGATGA CTTGTTCCCT GTCTTCTTAT ACGTGGTGCT GCGGGCCAGG 
ATTCGGAATT TAGGCTCTGA AGTTCATCTC ATTGAGGATC TGATGGACCC CTATCTCCAG 
CATGGGGAGC AAGGCATAAT GTTCACCACC TTGAAGGCCT GTTACTACCA GATTCAGCGT 
GAGAAGCTTA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452006.1
CDS	443..5377
Translation	MDSKKKSSAEAEGSKERGLVHVWQAGSFSLTPERLPGWGGKTVLQAALGVKHGVLLTEDGEVYSFGTLPWKSEAAEICPSSPLLESALVGHHVVTVATGSFHSGAVTESGVVYMWGENAAGQCAVANQQYVSEPSPVSISDSETSPLLAVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPPQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEKHASTVTSPHPETLDGQGEVFENTVAEAELNMGSDQTTSGSAISAQQNIVGMAEVSSARTAPSYPDTQTVTAYLQKLSEHSVKENHEREEKLPQVQPLVEEAVPDLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSTAPCEPGAPSGTASTGPESLKDLREEQVKQESLQGKKSSSLMDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGSHSLALTAKSQVYSWGSNTFGQLGHSEFPTTVPRLSKVSSESGVWSVAAGHGYSLFLVDTEDFQPGLYYSGRQDRAEGDTLPENPSGTTTPLGYISRVTAGKDSYLALVDKNIMGYIASLHELATTERRFYSKLSEIKSQILRPLLSLEHLGTVTTVQLLQEVASRFSKLCYLIGQHGASLSSYLQGMKEARNLVIMKHSSLFLDSYTEYCTSVSNFLVMGGSQLLAKPAIDFLNKNQELLQDLSEVNDENTQLMEILNALFFLPIRRLHNYAKVLLKLATCFEVTSPEYQKLQDSSSCYESLALHLGKKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEETGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGTSDFPLYGGSSTVQRQEPPISRSAKYTFYKDTRLKDATYDGRWLSGKPHGRGVLKWPDGKVYSGTFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGMWQDDACQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLCGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKAFRKLGNLAVAADEKWRAVFDECWRQLGCESPGQGEVWKAWDNIAVALTTNRRQHKDSPEILSRSQTQTLESLEYIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTVPLSAPLPTGRRSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALDNDREEDIYWECVLRLNKQPDIALLGFLGVQRKFWPATLSILGESKKVLPSTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_017596517.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596517.1

ORF Insert Sequence:

ATGGACTCAA AGAAGAAAAG CTCAGCAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGAGGA 
AAAACAGTTC TTCAGGCGGC CCTTGGCGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC CCTTCCCTGG AAAAGTGAAG CAGCAGAAAT TTGTCCAAGC 
AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTG TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCGGTGAC AGAAAGTGGA GTGGTGTACA TGTGGGGGGA GAACGCTGCC 
GGGCAGTGTG CAGTAGCGAA CCAGCAGTAT GTGTCGGAAC CAAGTCCTGT CAGCATTTCT 
GACTCGGAGA CCAGCCCCTT GCTAGCAGTT AGGATTCTGC AGTTGGCATG TGGCGAGGAA 
CACACACTGG CATTGTCAAT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG 
GGTCTCATCA CCACCACCTT CCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGA 
CGAGTGGTGC TCCAGGTGGC CTGTGGTGCG TTCCACAGCC TTGCACTTGT GCAGTGCCTC 
CCTCCCCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC 
ACCATGACAG ACAAAGAGGA CCACGTGATA ATATCAGACA GCCATTGCTG CCCTTTAGGT 
GTGACACTGT CTGAATCTCA AGCAGAAAAG CACGCCAGCA CTGTGACCAG CCCTCACCCG 
GAGACGCTGG ATGGGCAAGG AGAGGTGTTT GAGAACACAG TAGCAGAAGC TGAACTGAAT 
ATGGGAAGCG ATCAGACCAC AAGTGGCAGT GCCATTTCCG CCCAGCAGAA CATCGTGGGA 
ATGGCTGAAG TGTCTTCTGC CAGAACAGCT CCCTCATACC CAGACACCCA GACGGTAACT 
GCATACCTGC AGAAGCTATC AGAGCATTCA GTGAAGGAGA ACCATGAGCG TGAGGAAAAG 
CTACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC 
ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTGAGA 
GTGGCCGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTATAGC 
ACTGCCCCCT GCGAGCCAGG AGCTCCGTCA GGCACTGCCT CCACAGGCCC AGAAAGTCTG 
AAAGATCTCA GAGAAGAGCA GGTGAAACAG GAATCACTAC AAGGGAAGAA AAGCTCAAGC 
CTCATGGATA TCAGAGAGGA GGAGTCGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGA 
TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCCG CCAGGGTGAA AACACGGACA 
GTGGTTCTGA CTCCCACGTA CAGTGGAGAA GCAGATGCCC TCCTGCCTTC GCTGAGAACA 
GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTGG GGCACGGCGA TGTCCTGCCC 
AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTCATCCA CTTGGAGGCA 
GGTGGCTCCC ACTCTCTCGC ACTGACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT 
ACCTTCGGTC AGCTGGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCGAAGGTT 
AGCAGTGAAA GTGGAGTCTG GAGTGTAGCT GCAGGCCACG GTTATTCCCT GTTTTTAGTG 
GACACTGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GCCAGGACCG TGCAGAAGGT 
GACACCCTGC CAGAGAATCC CAGTGGTACA ACGACTCCGC TTGGATATAT CAGCAGAGTG 
ACAGCAGGAA AAGATAGCTA CCTAGCCTTG GTGGATAAGA ACATCATGGG ATACATCGCC 
AGCCTCCATG AGTTGGCTAC CACAGAAAGA CGGTTTTACT CGAAACTAAG TGAAATCAAG 
TCACAGATAC TTAGGCCTCT TCTCAGTCTG GAACATTTGG GCACTGTGAC CACTGTCCAG 
CTGTTGCAGG AAGTCGCCAG CCGGTTCAGC AAGTTGTGTT ACCTCATTGG GCAGCATGGA 
GCCTCACTGA GCAGCTACCT GCAAGGCATG AAGGAAGCCA GGAACCTGGT CATCATGAAG 
CACTCGAGTC TTTTCTTGGA CAGTTACACA GAGTATTGTA CATCAGTTTC AAATTTTCTG 
GTTATGGGAG GATCCCAGCT TCTTGCTAAG CCTGCAATTG ATTTCCTAAA TAAAAACCAA 
GAACTCTTGC AAGATTTGTC AGAAGTGAAT GATGAGAACA CTCAGTTGAT GGAAATACTG 
AATGCGCTGT TTTTCTTGCC AATCAGACGA CTTCATAATT ATGCAAAAGT TTTGCTAAAG 
CTCGCCACTT GCTTTGAAGT GACATCACCA GAGTATCAAA AGCTGCAGGA TTCCAGTTCT 
TGTTATGAGT CTCTTGCTCT CCATCTTGGC AAGAAGAGGA AGGAAGCAGA GTACACGCTG 
AGCTTCTGGA AGACCTTCCC TGGAAAAATG ACGGATTCCT TGAGGAAGCC AGAGCGCCGG 
CTGCTGTGTG AGAGCAGTAA CCGAGCCCTC TCCCTGCAGC ATGCTGGCAG GTTTTCTGTG 
AATTGGTTCA TTCTCTTCAA TGATGCCCTG GTCCATGCTC AGTTCTCCAC ACACCACGTG 
TTCCCTTTGG CCACACTCTG GGCAGAGCCA CTTTCTGAAG AAACTGGTGG CGTGAATGGC 
TTAAAGATAA CCACACCTGA AGAACAGTTC ACGCTCATTT CTTCAACACC CCAGGAAAAG 
ACCAAGTGGC TTCGGGCTAT CAGCCAAGCC GTGGACCAGG CTTTGAGGGG GACGTCTGAC 
TTCCCACTTT ACGGAGGCAG CAGCACTGTT CAGAGGCAGG AACCACCCAT CTCAAGAAGT 
GCCAAATACA CTTTCTACAA GGATACTCGC CTAAAGGACG CCACTTACGA TGGGCGCTGG 
CTTTCAGGGA AGCCGCACGG AAGGGGTGTT TTGAAGTGGC CTGATGGGAA GGTGTATTCT 
GGCACGTTCA GGAATGGCTT GGAAGACGGA TATGGTGAAT ACAGAATCCC AAACAAGGCC 
CTGAACAAAG AAGACCATTA CGTGGGCCAT TGGAAGGAGG GGAAAATGTG TGGGCAAGGA 
GTGTACAGCT ATGCCTCTGG TGAAGTGTTT GAAGGCTGCT TTCAAGATAA CATGCGCCAC 
GGGCACGGTC TCCTCCGCAG TGGAAAACTG ACTTCTTCCT CTCCCAGCAT GTTCATTGGC 
CAGTGGGTAA TGGACAAGAA AGCAGGGTAT GGCGTCTTCG ATGATATCAC CAGGGGAGAA 
AAGTACATGG GAATGTGGCA GGACGATGCA TGCCAAGGGA ATGGGGTGGT GGTCACCCAG 
TTTGGGTTAT ACTACGAAGG CAACTTCCAC CTTAATAAAA TGATGGGAAA TGGGGTTTTG 
CTTTCGGAAG ATGATACTAT ATATGAAGGA GAATTTTCTG ATGACTGGAC ACTTTGTGGA 
AAGGGAACAC TGACTATGCC AAATGGAGAT TATATTGAAG GTTATTTTAG TGGAGAATGG 
GGATCTGGGA TAAAAATCAC TGGGACCTAC TTCAAACCTA GCCTATATGA GAGTGATAAG 
GACAGGCCCA AAGCCTTCAG GAAGCTGGGA AACCTGGCCG TGGCCGCCGA TGAGAAATGG 
AGAGCAGTGT TTGACGAATG CTGGCGCCAG CTGGGCTGTG AGAGCCCAGG CCAAGGGGAG 
GTTTGGAAAG CGTGGGATAA TATTGCTGTG GCCTTGACCA CCAACCGTCG CCAGCATAAA 
GACAGTCCAG AAATACTAAG CCGTTCACAG ACTCAGACCC TGGAGAGTTT GGAGTACATT 
CCCCAGCACG TTGGCGCCTT CTCTGTGGAG AAATATGATG ATATCAAGAA GTATTTAATA 
AAGGCCTGTG ACACTCCTCT GCACCCACTG GGCAGGCTTG TGGAGACCCT GGTTGCAGTG 
TATAGAATGA CTTATGTGGG CGTAGGGGCC AACCGTCGGT TACTGCAGGA AGCTGTGAAG 
GAGATTAAGT CTTATCTCAA GAGGATTTTC CAACTTGTGA GGTTCTTGTT TCCTGAGCTT 
CCTGAAGAGG GCAGCACAGT TCCTCTCTCT GCTCCTCTGC CCACTGGAAG AAGATCCTTC 
TGCACTGGGA AATCAGATTC CAGATCCGAG TCACCAGAGC CAGGTTATGT AGTAACAAGT 
TCTGGCTTAC TGCTTCCGGT GCTGCTGCCT CGGCTCTACC CGCCTCTCTT CATGCTCTAT 
GCCCTGGACA ATGACCGAGA GGAAGACATT TACTGGGAAT GTGTGCTTCG ACTAAACAAG 
CAGCCAGATA TTGCTCTCCT GGGCTTTCTT GGAGTACAGA GGAAATTCTG GCCAGCAACC 
TTGTCAATCC TTGGAGAGAG TAAAAAGGTA CTGCCATCCA CAAAGGATGC TTGTTTTGCA 
TCTGCAGTAG AATGTCTGCA GCAGATCAGC ACAACATTTA CTCCATCAGA CAAACTTAAA 
GTGATCCAGC AGACCTTCGA AGAAATCTCT CAGAGTGTGC TGGCGTCGCT GCAGGAGGAC 
TTCCTTTGGT CCATGGATGA CTTGTTCCCT GTCTTCTTAT ACGTGGTGCT GCGGGCCAGG 
ATTCGGAATT TAGGCTCTGA AGTTCATCTC ATTGAGGATC TGATGGACCC CTATCTCCAG 
CATGGGGAGC AAGGCATAAT GTTCACCACC TTGAAGGCCT GTTACTACCA GATTCAGCGT 
GAGAAGCTTA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Als2 ( NM_001013413.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001013431.1 Rattus norvegicus alsin Rho guanine nucleotide exchange factor ALS2 (Als2), mRNA.

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CloneID	ORa03946
Clone ID Related Accession (Same CDS sequence)	XM_006245001.3 , NM_001013413.1
Accession Version	NM_001013413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	alsin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BK005190.1. On or before Feb 3, 2007 this sequence version replaced XM_343574.3, XM_001061827.1, XM_001064519.1, XM_001064573.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BK005190.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGACTCAA AGAAGAAAAG CTCAGCAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGAGGA 
AAAACAGTTC TTCAGGCGGC CCTTGGCGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC CCTTCCCTGG AAAAGTGAAG CAGCAGAAAT TTGTCCAAGC 
AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTG TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCGGTGAC AGAAAGTGGA GTGGTGTACA TGTGGGGGGA GAACGCTGCC 
GGGCAGTGTG CAGTAGCGAA CCAGCAGTAT GTGTCGGAAC CAAGTCCTGT CAGCATTTCT 
GACTCGGAGA CCAGCCCCTT GCTAGCAGTT AGGATTCTGC AGTTGGCATG TGGCGAGGAA 
CACACACTGG CATTGTCAAT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG 
GGTCTCATCA CCACCACCTT CCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGA 
CGAGTGGTGC TCCAGGTGGC CTGTGGTGCG TTCCACAGCC TTGCACTTGT GCAGTGCCTC 
CCTCCCCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC 
ACCATGACAG ACAAAGAGGA CCACGTGATA ATATCAGACA GCCATTGCTG CCCTTTAGGT 
GTGACACTGT CTGAATCTCA AGCAGAAAAG CACGCCAGCA CTGTGACCAG CCCTCACCCG 
GAGACGCTGG ATGGGCAAGG AGAGGTGTTT GAGAACACAG TAGCAGAAGC TGAACTGAAT 
ATGGGAAGCG ATCAGACCAC AAGTGGCAGT GCCATTTCCG CCCAGCAGAA CATCGTGGGA 
ATGGCTGAAG TGTCTTCTGC CAGAACAGCT CCCTCATACC CAGACACCCA GACGGTAACT 
GCATACCTGC AGAAGCTATC AGAGCATTCA GTGAAGGAGA ACCATGAGCG TGAGGAAAAG 
CTACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC 
ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTGAGA 
GTGGCCGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTATAGC 
ACTGCCCCCT GCGAGCCAGG AGCTCCGTCA GGCACTGCCT CCACAGGCCC AGAAAGTCTG 
AAAGATCTCA GAGAAGAGCA GGTGAAACAG GAATCACTAC AAGGGAAGAA AAGCTCAAGC 
CTCATGGATA TCAGAGAGGA GGAGTCGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGA 
TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCCG CCAGGGTGAA AACACGGACA 
GTGGTTCTGA CTCCCACGTA CAGTGGAGAA GCAGATGCCC TCCTGCCTTC GCTGAGAACA 
GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTGG GGCACGGCGA TGTCCTGCCC 
AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTCATCCA CTTGGAGGCA 
GGTGGCTCCC ACTCTCTCGC ACTGACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT 
ACCTTCGGTC AGCTGGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCGAAGGTT 
AGCAGTGAAA GTGGAGTCTG GAGTGTAGCT GCAGGCCACG GTTATTCCCT GTTTTTAGTG 
GACACTGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GCCAGGACCG TGCAGAAGGT 
GACACCCTGC CAGAGAATCC CAGTGGTACA ACGACTCCGG TACTTCTCTC CTGTAGTAAG 
CTTGGATATA TCAGCAGAGT GACAGCAGGA AAAGATAGCT ACCTAGCCTT GGTGGATAAG 
AACATCATGG GATACATCGC CAGCCTCCAT GAGTTGGCTA CCACAGAAAG ACGGTTTTAC 
TCGAAACTAA GTGAAATCAA GTCACAGATA CTTAGGCCTC TTCTCAGTCT GGAACATTTG 
GGCACTGTGA CCACTGTCCA GCTGTTGCAG GAAGTCGCCA GCCGGTTCAG CAAGTTGTGT 
TACCTCATTG GGCAGCATGG AGCCTCACTG AGCAGCTACC TGCAAGGCAT GAAGGAAGCC 
AGGAACCTGG TCATCATGAA GCACTCGAGT CTTTTCTTGG ACAGTTACAC AGAGTATTGT 
ACATCAGTTT CAAATTTTCT GGTTATGGGA GGATCCCAGC TTCTTGCTAA GCCTGCAATT 
GATTTCCTAA ATAAAAACCA AGAACTCTTG CAAGATTTGT CAGAAGTGAA TGATGAGAAC 
ACTCAGTTGA TGGAAATACT GAATGCGCTG TTTTTCTTGC CAATCAGACG ACTTCATAAT 
TATGCAAAAG TTTTGCTAAA GCTCGCCACT TGCTTTGAAG TGACATCACC AGAGTATCAA 
AAGCTGCAGG ATTCCAGTTC TTGTTATGAG TCTCTTGCTC TCCATCTTGG CAAGAAGAGG 
AAGGAAGCAG AGTACACGCT GAGCTTCTGG AAGACCTTCC CTGGAAAAAT GACGGATTCC 
TTGAGGAAGC CAGAGCGCCG GCTGCTGTGT GAGAGCAGTA ACCGAGCCCT CTCCCTGCAG 
CATGCTGGCA GGTTTTCTGT GAATTGGTTC ATTCTCTTCA ATGATGCCCT GGTCCATGCT 
CAGTTCTCCA CACACCACGT GTTCCCTTTG GCCACACTCT GGGCAGAGCC ACTTTCTGAA 
GAAACTGGTG GCGTGAATGG CTTAAAGATA ACCACACCTG AAGAACAGTT CACGCTCATT 
TCTTCAACAC CCCAGGAAAA GACCAAGTGG CTTCGGGCTA TCAGCCAAGC CGTGGACCAG 
GCTTTGAGGG GGACGTCTGA CTTCCCACTT TACGGAGGCA GCAGCACTGT TCAGAGGCAG 
GAACCACCCA TCTCAAGAAG TGCCAAATAC ACTTTCTACA AGGATACTCG CCTAAAGGAC 
GCCACTTACG ATGGGCGCTG GCTTTCAGGG AAGCCGCACG GAAGGGGTGT TTTGAAGTGG 
CCTGATGGGA AGGTGTATTC TGGCACGTTC AGGAATGGCT TGGAAGACGG ATATGGTGAA 
TACAGAATCC CAAACAAGGC CCTGAACAAA GAAGACCATT ACGTGGGCCA TTGGAAGGAG 
GGGAAAATGT GTGGGCAAGG AGTGTACAGC TATGCCTCTG GTGAAGTGTT TGAAGGCTGC 
TTTCAAGATA ACATGCGCCA CGGGCACGGT CTCCTCCGCA GTGGAAAACT GACTTCTTCC 
TCTCCCAGCA TGTTCATTGG CCAGTGGGTA ATGGACAAGA AAGCAGGGTA TGGCGTCTTC 
GATGATATCA CCAGGGGAGA AAAGTACATG GGAATGTGGC AGGACGATGC ATGCCAAGGG 
AATGGGGTGG TGGTCACCCA GTTTGGGTTA TACTACGAAG GCAACTTCCA CCTTAATAAA 
ATGATGGGAA ATGGGGTTTT GCTTTCGGAA GATGATACTA TATATGAAGG AGAATTTTCT 
GATGACTGGA CACTTTGTGG AAAGGGAACA CTGACTATGC CAAATGGAGA TTATATTGAA 
GGTTATTTTA GTGGAGAATG GGGATCTGGG ATAAAAATCA CTGGGACCTA CTTCAAACCT 
AGCCTATATG AGAGTGATAA GGACAGGCCC AAAGCCTTCA GGAAGCTGGG AAACCTGGCC 
GTGGCCGCCG ATGAGAAATG GAGAGCAGTG TTTGACGAAT GCTGGCGCCA GCTGGGCTGT 
GAGAGCCCAG GCCAAGGGGA GGTTTGGAAA GCGTGGGATA ATATTGCTGT GGCCTTGACC 
ACCAACCGTC GCCAGCATAA AGACAGTCCA GAAATACTAA GCCGTTCACA GACTCAGACC 
CTGGAGAGTT TGGAGTACAT TCCCCAGCAC GTTGGCGCCT TCTCTGTGGA GAAATATGAT 
GATATCAAGA AGTATTTAAT AAAGGCCTGT GACACTCCTC TGCACCCACT GGGCAGGCTT 
GTGGAGACCC TGGTTGCAGT GTATAGAATG ACTTATGTGG GCGTAGGGGC CAACCGTCGG 
TTACTGCAGG AAGCTGTGAA GGAGATTAAG TCTTATCTCA AGAGGATTTT CCAACTTGTG 
AGGTTCTTGT TTCCTGAGCT TCCTGAAGAG GGCAGCACAG TTCCTCTCTC TGCTCCTCTG 
CCCACTGGAA GAAGATCCTT CTGCACTGGG AAATCAGATT CCAGATCCGA GTCACCAGAG 
CCAGGTTATG TAGTAACAAG TTCTGGCTTA CTGCTTCCGG TGCTGCTGCC TCGGCTCTAC 
CCGCCTCTCT TCATGCTCTA TGCCCTGGAC AATGACCGAG AGGAAGACAT TTACTGGGAA 
TGTGTGCTTC GACTAAACAA GCAGCCAGAT ATTGCTCTCC TGGGCTTTCT TGGAGTACAG 
AGGAAATTCT GGCCAGCAAC CTTGTCAATC CTTGGAGAGA GTAAAAAGGT ACTGCCATCC 
ACAAAGGATG CTTGTTTTGC ATCTGCAGTA GAATGTCTGC AGCAGATCAG CACAACATTT 
ACTCCATCAG ACAAACTTAA AGTGATCCAG CAGACCTTCG AAGAAATCTC TCAGAGTGTG 
CTGGCGTCGC TGCAGGAGGA CTTCCTTTGG TCCATGGATG ACTTGTTCCC TGTCTTCTTA 
TACGTGGTGC TGCGGGCCAG GATTCGGAAT TTAGGCTCTG AAGTTCATCT CATTGAGGAT 
CTGATGGACC CCTATCTCCA GCATGGGGAG CAAGGCATAA TGTTCACCAC CTTGAAGGCC 
TGTTACTACC AGATTCAGCG TGAGAAGCTT AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013431.1
CDS	113..5068
Translation	MDSKKKSSAEAEGSKERGLVHVWQAGSFSLTPERLPGWGGKTVLQAALGVKHGVLLTEDGEVYSFGTLPWKSEAAEICPSSPLLESALVGHHVVTVATGSFHSGAVTESGVVYMWGENAAGQCAVANQQYVSEPSPVSISDSETSPLLAVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPPQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEKHASTVTSPHPETLDGQGEVFENTVAEAELNMGSDQTTSGSAISAQQNIVGMAEVSSARTAPSYPDTQTVTAYLQKLSEHSVKENHEREEKLPQVQPLVEEAVPDLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSTAPCEPGAPSGTASTGPESLKDLREEQVKQESLQGKKSSSLMDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGSHSLALTAKSQVYSWGSNTFGQLGHSEFPTTVPRLSKVSSESGVWSVAAGHGYSLFLVDTEDFQPGLYYSGRQDRAEGDTLPENPSGTTTPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELATTERRFYSKLSEIKSQILRPLLSLEHLGTVTTVQLLQEVASRFSKLCYLIGQHGASLSSYLQGMKEARNLVIMKHSSLFLDSYTEYCTSVSNFLVMGGSQLLAKPAIDFLNKNQELLQDLSEVNDENTQLMEILNALFFLPIRRLHNYAKVLLKLATCFEVTSPEYQKLQDSSSCYESLALHLGKKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEETGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGTSDFPLYGGSSTVQRQEPPISRSAKYTFYKDTRLKDATYDGRWLSGKPHGRGVLKWPDGKVYSGTFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGMWQDDACQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLCGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKAFRKLGNLAVAADEKWRAVFDECWRQLGCESPGQGEVWKAWDNIAVALTTNRRQHKDSPEILSRSQTQTLESLEYIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTVPLSAPLPTGRRSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALDNDREEDIYWECVLRLNKQPDIALLGFLGVQRKFWPATLSILGESKKVLPSTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	NM_001013413.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alsin Rho guanine nucleotide exchange factor ALS2 (Als2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013413.1

ORF Insert Sequence:

ATGGACTCAA AGAAGAAAAG CTCAGCAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGAGGA 
AAAACAGTTC TTCAGGCGGC CCTTGGCGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC CCTTCCCTGG AAAAGTGAAG CAGCAGAAAT TTGTCCAAGC 
AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTG TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCGGTGAC AGAAAGTGGA GTGGTGTACA TGTGGGGGGA GAACGCTGCC 
GGGCAGTGTG CAGTAGCGAA CCAGCAGTAT GTGTCGGAAC CAAGTCCTGT CAGCATTTCT 
GACTCGGAGA CCAGCCCCTT GCTAGCAGTT AGGATTCTGC AGTTGGCATG TGGCGAGGAA 
CACACACTGG CATTGTCAAT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG 
GGTCTCATCA CCACCACCTT CCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGA 
CGAGTGGTGC TCCAGGTGGC CTGTGGTGCG TTCCACAGCC TTGCACTTGT GCAGTGCCTC 
CCTCCCCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC 
ACCATGACAG ACAAAGAGGA CCACGTGATA ATATCAGACA GCCATTGCTG CCCTTTAGGT 
GTGACACTGT CTGAATCTCA AGCAGAAAAG CACGCCAGCA CTGTGACCAG CCCTCACCCG 
GAGACGCTGG ATGGGCAAGG AGAGGTGTTT GAGAACACAG TAGCAGAAGC TGAACTGAAT 
ATGGGAAGCG ATCAGACCAC AAGTGGCAGT GCCATTTCCG CCCAGCAGAA CATCGTGGGA 
ATGGCTGAAG TGTCTTCTGC CAGAACAGCT CCCTCATACC CAGACACCCA GACGGTAACT 
GCATACCTGC AGAAGCTATC AGAGCATTCA GTGAAGGAGA ACCATGAGCG TGAGGAAAAG 
CTACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC 
ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTGAGA 
GTGGCCGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTATAGC 
ACTGCCCCCT GCGAGCCAGG AGCTCCGTCA GGCACTGCCT CCACAGGCCC AGAAAGTCTG 
AAAGATCTCA GAGAAGAGCA GGTGAAACAG GAATCACTAC AAGGGAAGAA AAGCTCAAGC 
CTCATGGATA TCAGAGAGGA GGAGTCGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGA 
TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCCG CCAGGGTGAA AACACGGACA 
GTGGTTCTGA CTCCCACGTA CAGTGGAGAA GCAGATGCCC TCCTGCCTTC GCTGAGAACA 
GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTGG GGCACGGCGA TGTCCTGCCC 
AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTCATCCA CTTGGAGGCA 
GGTGGCTCCC ACTCTCTCGC ACTGACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT 
ACCTTCGGTC AGCTGGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCGAAGGTT 
AGCAGTGAAA GTGGAGTCTG GAGTGTAGCT GCAGGCCACG GTTATTCCCT GTTTTTAGTG 
GACACTGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GCCAGGACCG TGCAGAAGGT 
GACACCCTGC CAGAGAATCC CAGTGGTACA ACGACTCCGG TACTTCTCTC CTGTAGTAAG 
CTTGGATATA TCAGCAGAGT GACAGCAGGA AAAGATAGCT ACCTAGCCTT GGTGGATAAG 
AACATCATGG GATACATCGC CAGCCTCCAT GAGTTGGCTA CCACAGAAAG ACGGTTTTAC 
TCGAAACTAA GTGAAATCAA GTCACAGATA CTTAGGCCTC TTCTCAGTCT GGAACATTTG 
GGCACTGTGA CCACTGTCCA GCTGTTGCAG GAAGTCGCCA GCCGGTTCAG CAAGTTGTGT 
TACCTCATTG GGCAGCATGG AGCCTCACTG AGCAGCTACC TGCAAGGCAT GAAGGAAGCC 
AGGAACCTGG TCATCATGAA GCACTCGAGT CTTTTCTTGG ACAGTTACAC AGAGTATTGT 
ACATCAGTTT CAAATTTTCT GGTTATGGGA GGATCCCAGC TTCTTGCTAA GCCTGCAATT 
GATTTCCTAA ATAAAAACCA AGAACTCTTG CAAGATTTGT CAGAAGTGAA TGATGAGAAC 
ACTCAGTTGA TGGAAATACT GAATGCGCTG TTTTTCTTGC CAATCAGACG ACTTCATAAT 
TATGCAAAAG TTTTGCTAAA GCTCGCCACT TGCTTTGAAG TGACATCACC AGAGTATCAA 
AAGCTGCAGG ATTCCAGTTC TTGTTATGAG TCTCTTGCTC TCCATCTTGG CAAGAAGAGG 
AAGGAAGCAG AGTACACGCT GAGCTTCTGG AAGACCTTCC CTGGAAAAAT GACGGATTCC 
TTGAGGAAGC CAGAGCGCCG GCTGCTGTGT GAGAGCAGTA ACCGAGCCCT CTCCCTGCAG 
CATGCTGGCA GGTTTTCTGT GAATTGGTTC ATTCTCTTCA ATGATGCCCT GGTCCATGCT 
CAGTTCTCCA CACACCACGT GTTCCCTTTG GCCACACTCT GGGCAGAGCC ACTTTCTGAA 
GAAACTGGTG GCGTGAATGG CTTAAAGATA ACCACACCTG AAGAACAGTT CACGCTCATT 
TCTTCAACAC CCCAGGAAAA GACCAAGTGG CTTCGGGCTA TCAGCCAAGC CGTGGACCAG 
GCTTTGAGGG GGACGTCTGA CTTCCCACTT TACGGAGGCA GCAGCACTGT TCAGAGGCAG 
GAACCACCCA TCTCAAGAAG TGCCAAATAC ACTTTCTACA AGGATACTCG CCTAAAGGAC 
GCCACTTACG ATGGGCGCTG GCTTTCAGGG AAGCCGCACG GAAGGGGTGT TTTGAAGTGG 
CCTGATGGGA AGGTGTATTC TGGCACGTTC AGGAATGGCT TGGAAGACGG ATATGGTGAA 
TACAGAATCC CAAACAAGGC CCTGAACAAA GAAGACCATT ACGTGGGCCA TTGGAAGGAG 
GGGAAAATGT GTGGGCAAGG AGTGTACAGC TATGCCTCTG GTGAAGTGTT TGAAGGCTGC 
TTTCAAGATA ACATGCGCCA CGGGCACGGT CTCCTCCGCA GTGGAAAACT GACTTCTTCC 
TCTCCCAGCA TGTTCATTGG CCAGTGGGTA ATGGACAAGA AAGCAGGGTA TGGCGTCTTC 
GATGATATCA CCAGGGGAGA AAAGTACATG GGAATGTGGC AGGACGATGC ATGCCAAGGG 
AATGGGGTGG TGGTCACCCA GTTTGGGTTA TACTACGAAG GCAACTTCCA CCTTAATAAA 
ATGATGGGAA ATGGGGTTTT GCTTTCGGAA GATGATACTA TATATGAAGG AGAATTTTCT 
GATGACTGGA CACTTTGTGG AAAGGGAACA CTGACTATGC CAAATGGAGA TTATATTGAA 
GGTTATTTTA GTGGAGAATG GGGATCTGGG ATAAAAATCA CTGGGACCTA CTTCAAACCT 
AGCCTATATG AGAGTGATAA GGACAGGCCC AAAGCCTTCA GGAAGCTGGG AAACCTGGCC 
GTGGCCGCCG ATGAGAAATG GAGAGCAGTG TTTGACGAAT GCTGGCGCCA GCTGGGCTGT 
GAGAGCCCAG GCCAAGGGGA GGTTTGGAAA GCGTGGGATA ATATTGCTGT GGCCTTGACC 
ACCAACCGTC GCCAGCATAA AGACAGTCCA GAAATACTAA GCCGTTCACA GACTCAGACC 
CTGGAGAGTT TGGAGTACAT TCCCCAGCAC GTTGGCGCCT TCTCTGTGGA GAAATATGAT 
GATATCAAGA AGTATTTAAT AAAGGCCTGT GACACTCCTC TGCACCCACT GGGCAGGCTT 
GTGGAGACCC TGGTTGCAGT GTATAGAATG ACTTATGTGG GCGTAGGGGC CAACCGTCGG 
TTACTGCAGG AAGCTGTGAA GGAGATTAAG TCTTATCTCA AGAGGATTTT CCAACTTGTG 
AGGTTCTTGT TTCCTGAGCT TCCTGAAGAG GGCAGCACAG TTCCTCTCTC TGCTCCTCTG 
CCCACTGGAA GAAGATCCTT CTGCACTGGG AAATCAGATT CCAGATCCGA GTCACCAGAG 
CCAGGTTATG TAGTAACAAG TTCTGGCTTA CTGCTTCCGG TGCTGCTGCC TCGGCTCTAC 
CCGCCTCTCT TCATGCTCTA TGCCCTGGAC AATGACCGAG AGGAAGACAT TTACTGGGAA 
TGTGTGCTTC GACTAAACAA GCAGCCAGAT ATTGCTCTCC TGGGCTTTCT TGGAGTACAG 
AGGAAATTCT GGCCAGCAAC CTTGTCAATC CTTGGAGAGA GTAAAAAGGT ACTGCCATCC 
ACAAAGGATG CTTGTTTTGC ATCTGCAGTA GAATGTCTGC AGCAGATCAG CACAACATTT 
ACTCCATCAG ACAAACTTAA AGTGATCCAG CAGACCTTCG AAGAAATCTC TCAGAGTGTG 
CTGGCGTCGC TGCAGGAGGA CTTCCTTTGG TCCATGGATG ACTTGTTCCC TGTCTTCTTA 
TACGTGGTGC TGCGGGCCAG GATTCGGAAT TTAGGCTCTG AAGTTCATCT CATTGAGGAT 
CTGATGGACC CCTATCTCCA GCATGGGGAG CAAGGCATAA TGTTCACCAC CTTGAAGGCC 
TGTTACTACC AGATTCAGCG TGAGAAGCTT AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Als2 ( XM_006245001.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245063.1 Rattus norvegicus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant X1, mRNA.

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CloneID	ORa03946
Clone ID Related Accession (Same CDS sequence)	XM_006245001.3 , NM_001013413.1
Accession Version	XM_006245001.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245001.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAAAAG CTCAGCAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGAGGA 
AAAACAGTTC TTCAGGCGGC CCTTGGCGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC CCTTCCCTGG AAAAGTGAAG CAGCAGAAAT TTGTCCAAGC 
AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTG TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCGGTGAC AGAAAGTGGA GTGGTGTACA TGTGGGGGGA GAACGCTGCC 
GGGCAGTGTG CAGTAGCGAA CCAGCAGTAT GTGTCGGAAC CAAGTCCTGT CAGCATTTCT 
GACTCGGAGA CCAGCCCCTT GCTAGCAGTT AGGATTCTGC AGTTGGCATG TGGCGAGGAA 
CACACACTGG CATTGTCAAT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG 
GGTCTCATCA CCACCACCTT CCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGA 
CGAGTGGTGC TCCAGGTGGC CTGTGGTGCG TTCCACAGCC TTGCACTTGT GCAGTGCCTC 
CCTCCCCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC 
ACCATGACAG ACAAAGAGGA CCACGTGATA ATATCAGACA GCCATTGCTG CCCTTTAGGT 
GTGACACTGT CTGAATCTCA AGCAGAAAAG CACGCCAGCA CTGTGACCAG CCCTCACCCG 
GAGACGCTGG ATGGGCAAGG AGAGGTGTTT GAGAACACAG TAGCAGAAGC TGAACTGAAT 
ATGGGAAGCG ATCAGACCAC AAGTGGCAGT GCCATTTCCG CCCAGCAGAA CATCGTGGGA 
ATGGCTGAAG TGTCTTCTGC CAGAACAGCT CCCTCATACC CAGACACCCA GACGGTAACT 
GCATACCTGC AGAAGCTATC AGAGCATTCA GTGAAGGAGA ACCATGAGCG TGAGGAAAAG 
CTACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC 
ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTGAGA 
GTGGCCGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTATAGC 
ACTGCCCCCT GCGAGCCAGG AGCTCCGTCA GGCACTGCCT CCACAGGCCC AGAAAGTCTG 
AAAGATCTCA GAGAAGAGCA GGTGAAACAG GAATCACTAC AAGGGAAGAA AAGCTCAAGC 
CTCATGGATA TCAGAGAGGA GGAGTCGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGA 
TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCCG CCAGGGTGAA AACACGGACA 
GTGGTTCTGA CTCCCACGTA CAGTGGAGAA GCAGATGCCC TCCTGCCTTC GCTGAGAACA 
GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTGG GGCACGGCGA TGTCCTGCCC 
AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTCATCCA CTTGGAGGCA 
GGTGGCTCCC ACTCTCTCGC ACTGACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT 
ACCTTCGGTC AGCTGGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCGAAGGTT 
AGCAGTGAAA GTGGAGTCTG GAGTGTAGCT GCAGGCCACG GTTATTCCCT GTTTTTAGTG 
GACACTGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GCCAGGACCG TGCAGAAGGT 
GACACCCTGC CAGAGAATCC CAGTGGTACA ACGACTCCGG TACTTCTCTC CTGTAGTAAG 
CTTGGATATA TCAGCAGAGT GACAGCAGGA AAAGATAGCT ACCTAGCCTT GGTGGATAAG 
AACATCATGG GATACATCGC CAGCCTCCAT GAGTTGGCTA CCACAGAAAG ACGGTTTTAC 
TCGAAACTAA GTGAAATCAA GTCACAGATA CTTAGGCCTC TTCTCAGTCT GGAACATTTG 
GGCACTGTGA CCACTGTCCA GCTGTTGCAG GAAGTCGCCA GCCGGTTCAG CAAGTTGTGT 
TACCTCATTG GGCAGCATGG AGCCTCACTG AGCAGCTACC TGCAAGGCAT GAAGGAAGCC 
AGGAACCTGG TCATCATGAA GCACTCGAGT CTTTTCTTGG ACAGTTACAC AGAGTATTGT 
ACATCAGTTT CAAATTTTCT GGTTATGGGA GGATCCCAGC TTCTTGCTAA GCCTGCAATT 
GATTTCCTAA ATAAAAACCA AGAACTCTTG CAAGATTTGT CAGAAGTGAA TGATGAGAAC 
ACTCAGTTGA TGGAAATACT GAATGCGCTG TTTTTCTTGC CAATCAGACG ACTTCATAAT 
TATGCAAAAG TTTTGCTAAA GCTCGCCACT TGCTTTGAAG TGACATCACC AGAGTATCAA 
AAGCTGCAGG ATTCCAGTTC TTGTTATGAG TCTCTTGCTC TCCATCTTGG CAAGAAGAGG 
AAGGAAGCAG AGTACACGCT GAGCTTCTGG AAGACCTTCC CTGGAAAAAT GACGGATTCC 
TTGAGGAAGC CAGAGCGCCG GCTGCTGTGT GAGAGCAGTA ACCGAGCCCT CTCCCTGCAG 
CATGCTGGCA GGTTTTCTGT GAATTGGTTC ATTCTCTTCA ATGATGCCCT GGTCCATGCT 
CAGTTCTCCA CACACCACGT GTTCCCTTTG GCCACACTCT GGGCAGAGCC ACTTTCTGAA 
GAAACTGGTG GCGTGAATGG CTTAAAGATA ACCACACCTG AAGAACAGTT CACGCTCATT 
TCTTCAACAC CCCAGGAAAA GACCAAGTGG CTTCGGGCTA TCAGCCAAGC CGTGGACCAG 
GCTTTGAGGG GGACGTCTGA CTTCCCACTT TACGGAGGCA GCAGCACTGT TCAGAGGCAG 
GAACCACCCA TCTCAAGAAG TGCCAAATAC ACTTTCTACA AGGATACTCG CCTAAAGGAC 
GCCACTTACG ATGGGCGCTG GCTTTCAGGG AAGCCGCACG GAAGGGGTGT TTTGAAGTGG 
CCTGATGGGA AGGTGTATTC TGGCACGTTC AGGAATGGCT TGGAAGACGG ATATGGTGAA 
TACAGAATCC CAAACAAGGC CCTGAACAAA GAAGACCATT ACGTGGGCCA TTGGAAGGAG 
GGGAAAATGT GTGGGCAAGG AGTGTACAGC TATGCCTCTG GTGAAGTGTT TGAAGGCTGC 
TTTCAAGATA ACATGCGCCA CGGGCACGGT CTCCTCCGCA GTGGAAAACT GACTTCTTCC 
TCTCCCAGCA TGTTCATTGG CCAGTGGGTA ATGGACAAGA AAGCAGGGTA TGGCGTCTTC 
GATGATATCA CCAGGGGAGA AAAGTACATG GGAATGTGGC AGGACGATGC ATGCCAAGGG 
AATGGGGTGG TGGTCACCCA GTTTGGGTTA TACTACGAAG GCAACTTCCA CCTTAATAAA 
ATGATGGGAA ATGGGGTTTT GCTTTCGGAA GATGATACTA TATATGAAGG AGAATTTTCT 
GATGACTGGA CACTTTGTGG AAAGGGAACA CTGACTATGC CAAATGGAGA TTATATTGAA 
GGTTATTTTA GTGGAGAATG GGGATCTGGG ATAAAAATCA CTGGGACCTA CTTCAAACCT 
AGCCTATATG AGAGTGATAA GGACAGGCCC AAAGCCTTCA GGAAGCTGGG AAACCTGGCC 
GTGGCCGCCG ATGAGAAATG GAGAGCAGTG TTTGACGAAT GCTGGCGCCA GCTGGGCTGT 
GAGAGCCCAG GCCAAGGGGA GGTTTGGAAA GCGTGGGATA ATATTGCTGT GGCCTTGACC 
ACCAACCGTC GCCAGCATAA AGACAGTCCA GAAATACTAA GCCGTTCACA GACTCAGACC 
CTGGAGAGTT TGGAGTACAT TCCCCAGCAC GTTGGCGCCT TCTCTGTGGA GAAATATGAT 
GATATCAAGA AGTATTTAAT AAAGGCCTGT GACACTCCTC TGCACCCACT GGGCAGGCTT 
GTGGAGACCC TGGTTGCAGT GTATAGAATG ACTTATGTGG GCGTAGGGGC CAACCGTCGG 
TTACTGCAGG AAGCTGTGAA GGAGATTAAG TCTTATCTCA AGAGGATTTT CCAACTTGTG 
AGGTTCTTGT TTCCTGAGCT TCCTGAAGAG GGCAGCACAG TTCCTCTCTC TGCTCCTCTG 
CCCACTGGAA GAAGATCCTT CTGCACTGGG AAATCAGATT CCAGATCCGA GTCACCAGAG 
CCAGGTTATG TAGTAACAAG TTCTGGCTTA CTGCTTCCGG TGCTGCTGCC TCGGCTCTAC 
CCGCCTCTCT TCATGCTCTA TGCCCTGGAC AATGACCGAG AGGAAGACAT TTACTGGGAA 
TGTGTGCTTC GACTAAACAA GCAGCCAGAT ATTGCTCTCC TGGGCTTTCT TGGAGTACAG 
AGGAAATTCT GGCCAGCAAC CTTGTCAATC CTTGGAGAGA GTAAAAAGGT ACTGCCATCC 
ACAAAGGATG CTTGTTTTGC ATCTGCAGTA GAATGTCTGC AGCAGATCAG CACAACATTT 
ACTCCATCAG ACAAACTTAA AGTGATCCAG CAGACCTTCG AAGAAATCTC TCAGAGTGTG 
CTGGCGTCGC TGCAGGAGGA CTTCCTTTGG TCCATGGATG ACTTGTTCCC TGTCTTCTTA 
TACGTGGTGC TGCGGGCCAG GATTCGGAAT TTAGGCTCTG AAGTTCATCT CATTGAGGAT 
CTGATGGACC CCTATCTCCA GCATGGGGAG CAAGGCATAA TGTTCACCAC CTTGAAGGCC 
TGTTACTACC AGATTCAGCG TGAGAAGCTT AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245063.1
CDS	443..5398
Translation	MDSKKKSSAEAEGSKERGLVHVWQAGSFSLTPERLPGWGGKTVLQAALGVKHGVLLTEDGEVYSFGTLPWKSEAAEICPSSPLLESALVGHHVVTVATGSFHSGAVTESGVVYMWGENAAGQCAVANQQYVSEPSPVSISDSETSPLLAVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPPQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEKHASTVTSPHPETLDGQGEVFENTVAEAELNMGSDQTTSGSAISAQQNIVGMAEVSSARTAPSYPDTQTVTAYLQKLSEHSVKENHEREEKLPQVQPLVEEAVPDLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSTAPCEPGAPSGTASTGPESLKDLREEQVKQESLQGKKSSSLMDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGSHSLALTAKSQVYSWGSNTFGQLGHSEFPTTVPRLSKVSSESGVWSVAAGHGYSLFLVDTEDFQPGLYYSGRQDRAEGDTLPENPSGTTTPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELATTERRFYSKLSEIKSQILRPLLSLEHLGTVTTVQLLQEVASRFSKLCYLIGQHGASLSSYLQGMKEARNLVIMKHSSLFLDSYTEYCTSVSNFLVMGGSQLLAKPAIDFLNKNQELLQDLSEVNDENTQLMEILNALFFLPIRRLHNYAKVLLKLATCFEVTSPEYQKLQDSSSCYESLALHLGKKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEETGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGTSDFPLYGGSSTVQRQEPPISRSAKYTFYKDTRLKDATYDGRWLSGKPHGRGVLKWPDGKVYSGTFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGMWQDDACQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLCGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKAFRKLGNLAVAADEKWRAVFDECWRQLGCESPGQGEVWKAWDNIAVALTTNRRQHKDSPEILSRSQTQTLESLEYIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTVPLSAPLPTGRRSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALDNDREEDIYWECVLRLNKQPDIALLGFLGVQRKFWPATLSILGESKKVLPSTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_006245001.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245001.3

ORF Insert Sequence:

ATGGACTCAA AGAAGAAAAG CTCAGCAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGAGGA 
AAAACAGTTC TTCAGGCGGC CCTTGGCGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC CCTTCCCTGG AAAAGTGAAG CAGCAGAAAT TTGTCCAAGC 
AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTG TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCGGTGAC AGAAAGTGGA GTGGTGTACA TGTGGGGGGA GAACGCTGCC 
GGGCAGTGTG CAGTAGCGAA CCAGCAGTAT GTGTCGGAAC CAAGTCCTGT CAGCATTTCT 
GACTCGGAGA CCAGCCCCTT GCTAGCAGTT AGGATTCTGC AGTTGGCATG TGGCGAGGAA 
CACACACTGG CATTGTCAAT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG 
GGTCTCATCA CCACCACCTT CCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGA 
CGAGTGGTGC TCCAGGTGGC CTGTGGTGCG TTCCACAGCC TTGCACTTGT GCAGTGCCTC 
CCTCCCCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC 
ACCATGACAG ACAAAGAGGA CCACGTGATA ATATCAGACA GCCATTGCTG CCCTTTAGGT 
GTGACACTGT CTGAATCTCA AGCAGAAAAG CACGCCAGCA CTGTGACCAG CCCTCACCCG 
GAGACGCTGG ATGGGCAAGG AGAGGTGTTT GAGAACACAG TAGCAGAAGC TGAACTGAAT 
ATGGGAAGCG ATCAGACCAC AAGTGGCAGT GCCATTTCCG CCCAGCAGAA CATCGTGGGA 
ATGGCTGAAG TGTCTTCTGC CAGAACAGCT CCCTCATACC CAGACACCCA GACGGTAACT 
GCATACCTGC AGAAGCTATC AGAGCATTCA GTGAAGGAGA ACCATGAGCG TGAGGAAAAG 
CTACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC 
ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTGAGA 
GTGGCCGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTATAGC 
ACTGCCCCCT GCGAGCCAGG AGCTCCGTCA GGCACTGCCT CCACAGGCCC AGAAAGTCTG 
AAAGATCTCA GAGAAGAGCA GGTGAAACAG GAATCACTAC AAGGGAAGAA AAGCTCAAGC 
CTCATGGATA TCAGAGAGGA GGAGTCGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGA 
TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCCG CCAGGGTGAA AACACGGACA 
GTGGTTCTGA CTCCCACGTA CAGTGGAGAA GCAGATGCCC TCCTGCCTTC GCTGAGAACA 
GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTGG GGCACGGCGA TGTCCTGCCC 
AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTCATCCA CTTGGAGGCA 
GGTGGCTCCC ACTCTCTCGC ACTGACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT 
ACCTTCGGTC AGCTGGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCGAAGGTT 
AGCAGTGAAA GTGGAGTCTG GAGTGTAGCT GCAGGCCACG GTTATTCCCT GTTTTTAGTG 
GACACTGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GCCAGGACCG TGCAGAAGGT 
GACACCCTGC CAGAGAATCC CAGTGGTACA ACGACTCCGG TACTTCTCTC CTGTAGTAAG 
CTTGGATATA TCAGCAGAGT GACAGCAGGA AAAGATAGCT ACCTAGCCTT GGTGGATAAG 
AACATCATGG GATACATCGC CAGCCTCCAT GAGTTGGCTA CCACAGAAAG ACGGTTTTAC 
TCGAAACTAA GTGAAATCAA GTCACAGATA CTTAGGCCTC TTCTCAGTCT GGAACATTTG 
GGCACTGTGA CCACTGTCCA GCTGTTGCAG GAAGTCGCCA GCCGGTTCAG CAAGTTGTGT 
TACCTCATTG GGCAGCATGG AGCCTCACTG AGCAGCTACC TGCAAGGCAT GAAGGAAGCC 
AGGAACCTGG TCATCATGAA GCACTCGAGT CTTTTCTTGG ACAGTTACAC AGAGTATTGT 
ACATCAGTTT CAAATTTTCT GGTTATGGGA GGATCCCAGC TTCTTGCTAA GCCTGCAATT 
GATTTCCTAA ATAAAAACCA AGAACTCTTG CAAGATTTGT CAGAAGTGAA TGATGAGAAC 
ACTCAGTTGA TGGAAATACT GAATGCGCTG TTTTTCTTGC CAATCAGACG ACTTCATAAT 
TATGCAAAAG TTTTGCTAAA GCTCGCCACT TGCTTTGAAG TGACATCACC AGAGTATCAA 
AAGCTGCAGG ATTCCAGTTC TTGTTATGAG TCTCTTGCTC TCCATCTTGG CAAGAAGAGG 
AAGGAAGCAG AGTACACGCT GAGCTTCTGG AAGACCTTCC CTGGAAAAAT GACGGATTCC 
TTGAGGAAGC CAGAGCGCCG GCTGCTGTGT GAGAGCAGTA ACCGAGCCCT CTCCCTGCAG 
CATGCTGGCA GGTTTTCTGT GAATTGGTTC ATTCTCTTCA ATGATGCCCT GGTCCATGCT 
CAGTTCTCCA CACACCACGT GTTCCCTTTG GCCACACTCT GGGCAGAGCC ACTTTCTGAA 
GAAACTGGTG GCGTGAATGG CTTAAAGATA ACCACACCTG AAGAACAGTT CACGCTCATT 
TCTTCAACAC CCCAGGAAAA GACCAAGTGG CTTCGGGCTA TCAGCCAAGC CGTGGACCAG 
GCTTTGAGGG GGACGTCTGA CTTCCCACTT TACGGAGGCA GCAGCACTGT TCAGAGGCAG 
GAACCACCCA TCTCAAGAAG TGCCAAATAC ACTTTCTACA AGGATACTCG CCTAAAGGAC 
GCCACTTACG ATGGGCGCTG GCTTTCAGGG AAGCCGCACG GAAGGGGTGT TTTGAAGTGG 
CCTGATGGGA AGGTGTATTC TGGCACGTTC AGGAATGGCT TGGAAGACGG ATATGGTGAA 
TACAGAATCC CAAACAAGGC CCTGAACAAA GAAGACCATT ACGTGGGCCA TTGGAAGGAG 
GGGAAAATGT GTGGGCAAGG AGTGTACAGC TATGCCTCTG GTGAAGTGTT TGAAGGCTGC 
TTTCAAGATA ACATGCGCCA CGGGCACGGT CTCCTCCGCA GTGGAAAACT GACTTCTTCC 
TCTCCCAGCA TGTTCATTGG CCAGTGGGTA ATGGACAAGA AAGCAGGGTA TGGCGTCTTC 
GATGATATCA CCAGGGGAGA AAAGTACATG GGAATGTGGC AGGACGATGC ATGCCAAGGG 
AATGGGGTGG TGGTCACCCA GTTTGGGTTA TACTACGAAG GCAACTTCCA CCTTAATAAA 
ATGATGGGAA ATGGGGTTTT GCTTTCGGAA GATGATACTA TATATGAAGG AGAATTTTCT 
GATGACTGGA CACTTTGTGG AAAGGGAACA CTGACTATGC CAAATGGAGA TTATATTGAA 
GGTTATTTTA GTGGAGAATG GGGATCTGGG ATAAAAATCA CTGGGACCTA CTTCAAACCT 
AGCCTATATG AGAGTGATAA GGACAGGCCC AAAGCCTTCA GGAAGCTGGG AAACCTGGCC 
GTGGCCGCCG ATGAGAAATG GAGAGCAGTG TTTGACGAAT GCTGGCGCCA GCTGGGCTGT 
GAGAGCCCAG GCCAAGGGGA GGTTTGGAAA GCGTGGGATA ATATTGCTGT GGCCTTGACC 
ACCAACCGTC GCCAGCATAA AGACAGTCCA GAAATACTAA GCCGTTCACA GACTCAGACC 
CTGGAGAGTT TGGAGTACAT TCCCCAGCAC GTTGGCGCCT TCTCTGTGGA GAAATATGAT 
GATATCAAGA AGTATTTAAT AAAGGCCTGT GACACTCCTC TGCACCCACT GGGCAGGCTT 
GTGGAGACCC TGGTTGCAGT GTATAGAATG ACTTATGTGG GCGTAGGGGC CAACCGTCGG 
TTACTGCAGG AAGCTGTGAA GGAGATTAAG TCTTATCTCA AGAGGATTTT CCAACTTGTG 
AGGTTCTTGT TTCCTGAGCT TCCTGAAGAG GGCAGCACAG TTCCTCTCTC TGCTCCTCTG 
CCCACTGGAA GAAGATCCTT CTGCACTGGG AAATCAGATT CCAGATCCGA GTCACCAGAG 
CCAGGTTATG TAGTAACAAG TTCTGGCTTA CTGCTTCCGG TGCTGCTGCC TCGGCTCTAC 
CCGCCTCTCT TCATGCTCTA TGCCCTGGAC AATGACCGAG AGGAAGACAT TTACTGGGAA 
TGTGTGCTTC GACTAAACAA GCAGCCAGAT ATTGCTCTCC TGGGCTTTCT TGGAGTACAG 
AGGAAATTCT GGCCAGCAAC CTTGTCAATC CTTGGAGAGA GTAAAAAGGT ACTGCCATCC 
ACAAAGGATG CTTGTTTTGC ATCTGCAGTA GAATGTCTGC AGCAGATCAG CACAACATTT 
ACTCCATCAG ACAAACTTAA AGTGATCCAG CAGACCTTCG AAGAAATCTC TCAGAGTGTG 
CTGGCGTCGC TGCAGGAGGA CTTCCTTTGG TCCATGGATG ACTTGTTCCC TGTCTTCTTA 
TACGTGGTGC TGCGGGCCAG GATTCGGAAT TTAGGCTCTG AAGTTCATCT CATTGAGGAT 
CTGATGGACC CCTATCTCCA GCATGGGGAG CAAGGCATAA TGTTCACCAC CTTGAAGGCC 
TGTTACTACC AGATTCAGCG TGAGAAGCTT AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular interaction of neurocalcin alpha with alsin (ALS2).
Neuroscience letters438(1)26-8(2008 Jun)
Masutani T,Taguchi K,Kumanogoh H,Nakamura S,Maekawa S

ALS2CL, a novel ALS2-interactor, modulates ALS2-mediated endosome dynamics.
Biochemical and biophysical research communications354(2)491-7(2007 Mar)
Suzuki-Utsunomiya K,Hadano S,Otomo A,Kunita R,Mizumura H,Osuga H,Ikeda JE

Amyotrophic lateral sclerosis 2-deficiency leads to neuronal degeneration in amyotrophic lateral sclerosis through altered AMPA receptor trafficking.
The Journal of neuroscience : the official journal of the Society for Neuroscience26(45)11798-806(2006 Nov)
Lai C,Xie C,McCormack SG,Chiang HC,Michalak MK,Lin X,Chandran J,Shim H,Shimoji M,Cookson MR,Huganir RL,Rothstein JD,Price DL,Wong PC,Martin LJ,Zhu JJ,Cai H

Alsin/Rac1 signaling controls survival and growth of spinal motoneurons.
Annals of neurology60(1)105-17(2006 Jul)
Jacquier A,Buhler E,Sch?fer MK,Bohl D,Blanchard S,Beclin C,Haase G

Als2-deficient mice exhibit disturbances in endosome trafficking associated with motor behavioral abnormalities.
Proceedings of the National Academy of Sciences of the United States of America103(25)9595-600(2006 Jun)
Devon RS,Orban PC,Gerrow K,Barbieri MA,Schwab C,Cao LP,Helm JR,Bissada N,Cruz-Aguado R,Davidson TL,Witmer J,Metzler M,Lam CK,Tetzlaff W,Simpson EM,McCaffery JM,El-Husseini AE,Leavitt BR,Hayden MR

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Als2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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