Als2 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Als2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Als2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa48506 XM_008767118.2
Latest version!
Rattus norvegicus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
ORa62808 XM_017596517.1
Latest version!
Rattus norvegicus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
ORa03946 NM_001013413.1
Latest version!
Rattus norvegicus alsin Rho guanine nucleotide exchange factor ALS2 (Als2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
ORa03946 XM_006245001.3
Latest version!
Rattus norvegicus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa48506
    Clone ID Related Accession (Same CDS sequence) XM_008767118.2
    Accession Version XM_008767118.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product alsin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767118.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACTCAA AGAAGAAAAG CTCAGCAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
    CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGAGGA
    AAAACAGTTC TTCAGGCGGC CCTTGGCGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC CCTTCCCTGG AAAAGTGAAG CAGCAGAAAT TTGTCCAAGC
    AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTG TTACTGTGGC AACAGGGAGC
    TTCCACAGTG GAGCGGTGAC AGAAAGTGGA GTGGTGTACA TGTGGGGGGA GAACGCTGCC
    GGGCAGTGTG CAGTAGCGAA CCAGCAGTAT GTGTCGGAAC CAAGTCCTGT CAGCATTTCT
    GACTCGGAGA CCAGCCCCTT GCTAGCAGTT AGGATTCTGC AGTTGGCATG TGGCGAGGAA
    CACACACTGG CATTGTCAAT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG
    GGTCTCATCA CCACCACCTT CCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGA
    CGAGTGGTGC TCCAGGTGGC CTGTGGTGCG TTCCACAGCC TTGCACTTGT GCAGTGCCTC
    CCTCCCCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC
    ACCATGACAG ACAAAGAGGA CCACGTGATA ATATCAGACA GCCATTGCTG CCCTTTAGGT
    GTGACACTGT CTGAATCTCA AGCAGAAAAG CACGCCAGCA CTGTGACCAG CCCTCACCCG
    GAGACGCTGG ATGGGCAAGG AGAGGTGTTT GAGAACACAG TAGCAGAAGC TGAACTGAAT
    ATGGGAAGCG ATCAGACCAC AAGTGGCAGT GCCATTTCCG CCCAGCAGAA CATCGTGGGA
    ATGGCTGAAG TGTCTTCTGC CAGAACAGCT CCCTCATACC CAGACACCCA GACGGTAACT
    GCATACCTGC AGAAGCTATC AGAGCATTCA GTGAAGGAGA ACCATGAGCG TGAGGAAAAG
    CTACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC
    ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTGAGA
    GTGGCCGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTATAGC
    ACTGCCCCCT GCGAGCCAGG AGCTCCGTCA GGCACTGCCT CCACAGGCCC AGAAAGTCTG
    AAAGATCTCA GAGAAGAGCA GGTGAAACAG GAATCACTAC AAGGGAAGAA AAGCTCAAGC
    CTCATGGATA TCAGAGAGGA GGAGTCGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGA
    TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCCG CCAGGGTGAA AACACGGACA
    GTGGTTCTGA CTCCCACGTA CAGTGGAGAA GCAGATGCCC TCCTGCCTTC GCTGAGAACA
    GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTGG GGCACGGCGA TGTCCTGCCC
    AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTCATCCA CTTGGAGGCA
    GGTGGCTCCC ACTCTCTCGC ACTGACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT
    ACCTTCGGTC AGCTGGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCGAAGGTT
    AGCAGTGAAA GTGGAGTCTG GAGTGTAGCT GCAGGCCACG GTTATTCCCT GTTTTTAGTG
    GACACTGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GCCAGGACCG TGCAGAAGGT
    GACACCCTGC CAGAGAATCC CAGTGGTACA ACGACTCCGG TACTTCTCTC CTGTAGTAAG
    GATTTTCTCC GTGTGACTCT GACTGGCCCC TCAGGAACTC GCTCTGTAGA CCAGCCTGGC
    CTCAAACTCA CAGAGATCTG CCTGCCTCTG CCTCCAGAGT GCTGGGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765340.1
    CDS448..2538
    Translation

    Target ORF information:

    RefSeq Version XM_008767118.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767118.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACTCAA AGAAGAAAAG CTCAGCAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
    CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGAGGA
    AAAACAGTTC TTCAGGCGGC CCTTGGCGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC CCTTCCCTGG AAAAGTGAAG CAGCAGAAAT TTGTCCAAGC
    AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTG TTACTGTGGC AACAGGGAGC
    TTCCACAGTG GAGCGGTGAC AGAAAGTGGA GTGGTGTACA TGTGGGGGGA GAACGCTGCC
    GGGCAGTGTG CAGTAGCGAA CCAGCAGTAT GTGTCGGAAC CAAGTCCTGT CAGCATTTCT
    GACTCGGAGA CCAGCCCCTT GCTAGCAGTT AGGATTCTGC AGTTGGCATG TGGCGAGGAA
    CACACACTGG CATTGTCAAT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG
    GGTCTCATCA CCACCACCTT CCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGA
    CGAGTGGTGC TCCAGGTGGC CTGTGGTGCG TTCCACAGCC TTGCACTTGT GCAGTGCCTC
    CCTCCCCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC
    ACCATGACAG ACAAAGAGGA CCACGTGATA ATATCAGACA GCCATTGCTG CCCTTTAGGT
    GTGACACTGT CTGAATCTCA AGCAGAAAAG CACGCCAGCA CTGTGACCAG CCCTCACCCG
    GAGACGCTGG ATGGGCAAGG AGAGGTGTTT GAGAACACAG TAGCAGAAGC TGAACTGAAT
    ATGGGAAGCG ATCAGACCAC AAGTGGCAGT GCCATTTCCG CCCAGCAGAA CATCGTGGGA
    ATGGCTGAAG TGTCTTCTGC CAGAACAGCT CCCTCATACC CAGACACCCA GACGGTAACT
    GCATACCTGC AGAAGCTATC AGAGCATTCA GTGAAGGAGA ACCATGAGCG TGAGGAAAAG
    CTACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC
    ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTGAGA
    GTGGCCGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTATAGC
    ACTGCCCCCT GCGAGCCAGG AGCTCCGTCA GGCACTGCCT CCACAGGCCC AGAAAGTCTG
    AAAGATCTCA GAGAAGAGCA GGTGAAACAG GAATCACTAC AAGGGAAGAA AAGCTCAAGC
    CTCATGGATA TCAGAGAGGA GGAGTCGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGA
    TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCCG CCAGGGTGAA AACACGGACA
    GTGGTTCTGA CTCCCACGTA CAGTGGAGAA GCAGATGCCC TCCTGCCTTC GCTGAGAACA
    GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTGG GGCACGGCGA TGTCCTGCCC
    AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTCATCCA CTTGGAGGCA
    GGTGGCTCCC ACTCTCTCGC ACTGACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT
    ACCTTCGGTC AGCTGGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCGAAGGTT
    AGCAGTGAAA GTGGAGTCTG GAGTGTAGCT GCAGGCCACG GTTATTCCCT GTTTTTAGTG
    GACACTGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GCCAGGACCG TGCAGAAGGT
    GACACCCTGC CAGAGAATCC CAGTGGTACA ACGACTCCGG TACTTCTCTC CTGTAGTAAG
    GATTTTCTCC GTGTGACTCT GACTGGCCCC TCAGGAACTC GCTCTGTAGA CCAGCCTGGC
    CTCAAACTCA CAGAGATCTG CCTGCCTCTG CCTCCAGAGT GCTGGGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62808
    Clone ID Related Accession (Same CDS sequence) XM_017596517.1
    Accession Version XM_017596517.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product alsin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAAAAG CTCAGCAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
    CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGAGGA
    AAAACAGTTC TTCAGGCGGC CCTTGGCGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC CCTTCCCTGG AAAAGTGAAG CAGCAGAAAT TTGTCCAAGC
    AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTG TTACTGTGGC AACAGGGAGC
    TTCCACAGTG GAGCGGTGAC AGAAAGTGGA GTGGTGTACA TGTGGGGGGA GAACGCTGCC
    GGGCAGTGTG CAGTAGCGAA CCAGCAGTAT GTGTCGGAAC CAAGTCCTGT CAGCATTTCT
    GACTCGGAGA CCAGCCCCTT GCTAGCAGTT AGGATTCTGC AGTTGGCATG TGGCGAGGAA
    CACACACTGG CATTGTCAAT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG
    GGTCTCATCA CCACCACCTT CCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGA
    CGAGTGGTGC TCCAGGTGGC CTGTGGTGCG TTCCACAGCC TTGCACTTGT GCAGTGCCTC
    CCTCCCCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC
    ACCATGACAG ACAAAGAGGA CCACGTGATA ATATCAGACA GCCATTGCTG CCCTTTAGGT
    GTGACACTGT CTGAATCTCA AGCAGAAAAG CACGCCAGCA CTGTGACCAG CCCTCACCCG
    GAGACGCTGG ATGGGCAAGG AGAGGTGTTT GAGAACACAG TAGCAGAAGC TGAACTGAAT
    ATGGGAAGCG ATCAGACCAC AAGTGGCAGT GCCATTTCCG CCCAGCAGAA CATCGTGGGA
    ATGGCTGAAG TGTCTTCTGC CAGAACAGCT CCCTCATACC CAGACACCCA GACGGTAACT
    GCATACCTGC AGAAGCTATC AGAGCATTCA GTGAAGGAGA ACCATGAGCG TGAGGAAAAG
    CTACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC
    ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTGAGA
    GTGGCCGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTATAGC
    ACTGCCCCCT GCGAGCCAGG AGCTCCGTCA GGCACTGCCT CCACAGGCCC AGAAAGTCTG
    AAAGATCTCA GAGAAGAGCA GGTGAAACAG GAATCACTAC AAGGGAAGAA AAGCTCAAGC
    CTCATGGATA TCAGAGAGGA GGAGTCGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGA
    TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCCG CCAGGGTGAA AACACGGACA
    GTGGTTCTGA CTCCCACGTA CAGTGGAGAA GCAGATGCCC TCCTGCCTTC GCTGAGAACA
    GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTGG GGCACGGCGA TGTCCTGCCC
    AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTCATCCA CTTGGAGGCA
    GGTGGCTCCC ACTCTCTCGC ACTGACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT
    ACCTTCGGTC AGCTGGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCGAAGGTT
    AGCAGTGAAA GTGGAGTCTG GAGTGTAGCT GCAGGCCACG GTTATTCCCT GTTTTTAGTG
    GACACTGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GCCAGGACCG TGCAGAAGGT
    GACACCCTGC CAGAGAATCC CAGTGGTACA ACGACTCCGC TTGGATATAT CAGCAGAGTG
    ACAGCAGGAA AAGATAGCTA CCTAGCCTTG GTGGATAAGA ACATCATGGG ATACATCGCC
    AGCCTCCATG AGTTGGCTAC CACAGAAAGA CGGTTTTACT CGAAACTAAG TGAAATCAAG
    TCACAGATAC TTAGGCCTCT TCTCAGTCTG GAACATTTGG GCACTGTGAC CACTGTCCAG
    CTGTTGCAGG AAGTCGCCAG CCGGTTCAGC AAGTTGTGTT ACCTCATTGG GCAGCATGGA
    GCCTCACTGA GCAGCTACCT GCAAGGCATG AAGGAAGCCA GGAACCTGGT CATCATGAAG
    CACTCGAGTC TTTTCTTGGA CAGTTACACA GAGTATTGTA CATCAGTTTC AAATTTTCTG
    GTTATGGGAG GATCCCAGCT TCTTGCTAAG CCTGCAATTG ATTTCCTAAA TAAAAACCAA
    GAACTCTTGC AAGATTTGTC AGAAGTGAAT GATGAGAACA CTCAGTTGAT GGAAATACTG
    AATGCGCTGT TTTTCTTGCC AATCAGACGA CTTCATAATT ATGCAAAAGT TTTGCTAAAG
    CTCGCCACTT GCTTTGAAGT GACATCACCA GAGTATCAAA AGCTGCAGGA TTCCAGTTCT
    TGTTATGAGT CTCTTGCTCT CCATCTTGGC AAGAAGAGGA AGGAAGCAGA GTACACGCTG
    AGCTTCTGGA AGACCTTCCC TGGAAAAATG ACGGATTCCT TGAGGAAGCC AGAGCGCCGG
    CTGCTGTGTG AGAGCAGTAA CCGAGCCCTC TCCCTGCAGC ATGCTGGCAG GTTTTCTGTG
    AATTGGTTCA TTCTCTTCAA TGATGCCCTG GTCCATGCTC AGTTCTCCAC ACACCACGTG
    TTCCCTTTGG CCACACTCTG GGCAGAGCCA CTTTCTGAAG AAACTGGTGG CGTGAATGGC
    TTAAAGATAA CCACACCTGA AGAACAGTTC ACGCTCATTT CTTCAACACC CCAGGAAAAG
    ACCAAGTGGC TTCGGGCTAT CAGCCAAGCC GTGGACCAGG CTTTGAGGGG GACGTCTGAC
    TTCCCACTTT ACGGAGGCAG CAGCACTGTT CAGAGGCAGG AACCACCCAT CTCAAGAAGT
    GCCAAATACA CTTTCTACAA GGATACTCGC CTAAAGGACG CCACTTACGA TGGGCGCTGG
    CTTTCAGGGA AGCCGCACGG AAGGGGTGTT TTGAAGTGGC CTGATGGGAA GGTGTATTCT
    GGCACGTTCA GGAATGGCTT GGAAGACGGA TATGGTGAAT ACAGAATCCC AAACAAGGCC
    CTGAACAAAG AAGACCATTA CGTGGGCCAT TGGAAGGAGG GGAAAATGTG TGGGCAAGGA
    GTGTACAGCT ATGCCTCTGG TGAAGTGTTT GAAGGCTGCT TTCAAGATAA CATGCGCCAC
    GGGCACGGTC TCCTCCGCAG TGGAAAACTG ACTTCTTCCT CTCCCAGCAT GTTCATTGGC
    CAGTGGGTAA TGGACAAGAA AGCAGGGTAT GGCGTCTTCG ATGATATCAC CAGGGGAGAA
    AAGTACATGG GAATGTGGCA GGACGATGCA TGCCAAGGGA ATGGGGTGGT GGTCACCCAG
    TTTGGGTTAT ACTACGAAGG CAACTTCCAC CTTAATAAAA TGATGGGAAA TGGGGTTTTG
    CTTTCGGAAG ATGATACTAT ATATGAAGGA GAATTTTCTG ATGACTGGAC ACTTTGTGGA
    AAGGGAACAC TGACTATGCC AAATGGAGAT TATATTGAAG GTTATTTTAG TGGAGAATGG
    GGATCTGGGA TAAAAATCAC TGGGACCTAC TTCAAACCTA GCCTATATGA GAGTGATAAG
    GACAGGCCCA AAGCCTTCAG GAAGCTGGGA AACCTGGCCG TGGCCGCCGA TGAGAAATGG
    AGAGCAGTGT TTGACGAATG CTGGCGCCAG CTGGGCTGTG AGAGCCCAGG CCAAGGGGAG
    GTTTGGAAAG CGTGGGATAA TATTGCTGTG GCCTTGACCA CCAACCGTCG CCAGCATAAA
    GACAGTCCAG AAATACTAAG CCGTTCACAG ACTCAGACCC TGGAGAGTTT GGAGTACATT
    CCCCAGCACG TTGGCGCCTT CTCTGTGGAG AAATATGATG ATATCAAGAA GTATTTAATA
    AAGGCCTGTG ACACTCCTCT GCACCCACTG GGCAGGCTTG TGGAGACCCT GGTTGCAGTG
    TATAGAATGA CTTATGTGGG CGTAGGGGCC AACCGTCGGT TACTGCAGGA AGCTGTGAAG
    GAGATTAAGT CTTATCTCAA GAGGATTTTC CAACTTGTGA GGTTCTTGTT TCCTGAGCTT
    CCTGAAGAGG GCAGCACAGT TCCTCTCTCT GCTCCTCTGC CCACTGGAAG AAGATCCTTC
    TGCACTGGGA AATCAGATTC CAGATCCGAG TCACCAGAGC CAGGTTATGT AGTAACAAGT
    TCTGGCTTAC TGCTTCCGGT GCTGCTGCCT CGGCTCTACC CGCCTCTCTT CATGCTCTAT
    GCCCTGGACA ATGACCGAGA GGAAGACATT TACTGGGAAT GTGTGCTTCG ACTAAACAAG
    CAGCCAGATA TTGCTCTCCT GGGCTTTCTT GGAGTACAGA GGAAATTCTG GCCAGCAACC
    TTGTCAATCC TTGGAGAGAG TAAAAAGGTA CTGCCATCCA CAAAGGATGC TTGTTTTGCA
    TCTGCAGTAG AATGTCTGCA GCAGATCAGC ACAACATTTA CTCCATCAGA CAAACTTAAA
    GTGATCCAGC AGACCTTCGA AGAAATCTCT CAGAGTGTGC TGGCGTCGCT GCAGGAGGAC
    TTCCTTTGGT CCATGGATGA CTTGTTCCCT GTCTTCTTAT ACGTGGTGCT GCGGGCCAGG
    ATTCGGAATT TAGGCTCTGA AGTTCATCTC ATTGAGGATC TGATGGACCC CTATCTCCAG
    CATGGGGAGC AAGGCATAAT GTTCACCACC TTGAAGGCCT GTTACTACCA GATTCAGCGT
    GAGAAGCTTA ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017452006.1
    CDS443..5377
    Translation

    Target ORF information:

    RefSeq Version XM_017596517.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017596517.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAAAAG CTCAGCAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
    CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGAGGA
    AAAACAGTTC TTCAGGCGGC CCTTGGCGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC CCTTCCCTGG AAAAGTGAAG CAGCAGAAAT TTGTCCAAGC
    AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTG TTACTGTGGC AACAGGGAGC
    TTCCACAGTG GAGCGGTGAC AGAAAGTGGA GTGGTGTACA TGTGGGGGGA GAACGCTGCC
    GGGCAGTGTG CAGTAGCGAA CCAGCAGTAT GTGTCGGAAC CAAGTCCTGT CAGCATTTCT
    GACTCGGAGA CCAGCCCCTT GCTAGCAGTT AGGATTCTGC AGTTGGCATG TGGCGAGGAA
    CACACACTGG CATTGTCAAT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG
    GGTCTCATCA CCACCACCTT CCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGA
    CGAGTGGTGC TCCAGGTGGC CTGTGGTGCG TTCCACAGCC TTGCACTTGT GCAGTGCCTC
    CCTCCCCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC
    ACCATGACAG ACAAAGAGGA CCACGTGATA ATATCAGACA GCCATTGCTG CCCTTTAGGT
    GTGACACTGT CTGAATCTCA AGCAGAAAAG CACGCCAGCA CTGTGACCAG CCCTCACCCG
    GAGACGCTGG ATGGGCAAGG AGAGGTGTTT GAGAACACAG TAGCAGAAGC TGAACTGAAT
    ATGGGAAGCG ATCAGACCAC AAGTGGCAGT GCCATTTCCG CCCAGCAGAA CATCGTGGGA
    ATGGCTGAAG TGTCTTCTGC CAGAACAGCT CCCTCATACC CAGACACCCA GACGGTAACT
    GCATACCTGC AGAAGCTATC AGAGCATTCA GTGAAGGAGA ACCATGAGCG TGAGGAAAAG
    CTACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC
    ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTGAGA
    GTGGCCGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTATAGC
    ACTGCCCCCT GCGAGCCAGG AGCTCCGTCA GGCACTGCCT CCACAGGCCC AGAAAGTCTG
    AAAGATCTCA GAGAAGAGCA GGTGAAACAG GAATCACTAC AAGGGAAGAA AAGCTCAAGC
    CTCATGGATA TCAGAGAGGA GGAGTCGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGA
    TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCCG CCAGGGTGAA AACACGGACA
    GTGGTTCTGA CTCCCACGTA CAGTGGAGAA GCAGATGCCC TCCTGCCTTC GCTGAGAACA
    GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTGG GGCACGGCGA TGTCCTGCCC
    AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTCATCCA CTTGGAGGCA
    GGTGGCTCCC ACTCTCTCGC ACTGACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT
    ACCTTCGGTC AGCTGGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCGAAGGTT
    AGCAGTGAAA GTGGAGTCTG GAGTGTAGCT GCAGGCCACG GTTATTCCCT GTTTTTAGTG
    GACACTGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GCCAGGACCG TGCAGAAGGT
    GACACCCTGC CAGAGAATCC CAGTGGTACA ACGACTCCGC TTGGATATAT CAGCAGAGTG
    ACAGCAGGAA AAGATAGCTA CCTAGCCTTG GTGGATAAGA ACATCATGGG ATACATCGCC
    AGCCTCCATG AGTTGGCTAC CACAGAAAGA CGGTTTTACT CGAAACTAAG TGAAATCAAG
    TCACAGATAC TTAGGCCTCT TCTCAGTCTG GAACATTTGG GCACTGTGAC CACTGTCCAG
    CTGTTGCAGG AAGTCGCCAG CCGGTTCAGC AAGTTGTGTT ACCTCATTGG GCAGCATGGA
    GCCTCACTGA GCAGCTACCT GCAAGGCATG AAGGAAGCCA GGAACCTGGT CATCATGAAG
    CACTCGAGTC TTTTCTTGGA CAGTTACACA GAGTATTGTA CATCAGTTTC AAATTTTCTG
    GTTATGGGAG GATCCCAGCT TCTTGCTAAG CCTGCAATTG ATTTCCTAAA TAAAAACCAA
    GAACTCTTGC AAGATTTGTC AGAAGTGAAT GATGAGAACA CTCAGTTGAT GGAAATACTG
    AATGCGCTGT TTTTCTTGCC AATCAGACGA CTTCATAATT ATGCAAAAGT TTTGCTAAAG
    CTCGCCACTT GCTTTGAAGT GACATCACCA GAGTATCAAA AGCTGCAGGA TTCCAGTTCT
    TGTTATGAGT CTCTTGCTCT CCATCTTGGC AAGAAGAGGA AGGAAGCAGA GTACACGCTG
    AGCTTCTGGA AGACCTTCCC TGGAAAAATG ACGGATTCCT TGAGGAAGCC AGAGCGCCGG
    CTGCTGTGTG AGAGCAGTAA CCGAGCCCTC TCCCTGCAGC ATGCTGGCAG GTTTTCTGTG
    AATTGGTTCA TTCTCTTCAA TGATGCCCTG GTCCATGCTC AGTTCTCCAC ACACCACGTG
    TTCCCTTTGG CCACACTCTG GGCAGAGCCA CTTTCTGAAG AAACTGGTGG CGTGAATGGC
    TTAAAGATAA CCACACCTGA AGAACAGTTC ACGCTCATTT CTTCAACACC CCAGGAAAAG
    ACCAAGTGGC TTCGGGCTAT CAGCCAAGCC GTGGACCAGG CTTTGAGGGG GACGTCTGAC
    TTCCCACTTT ACGGAGGCAG CAGCACTGTT CAGAGGCAGG AACCACCCAT CTCAAGAAGT
    GCCAAATACA CTTTCTACAA GGATACTCGC CTAAAGGACG CCACTTACGA TGGGCGCTGG
    CTTTCAGGGA AGCCGCACGG AAGGGGTGTT TTGAAGTGGC CTGATGGGAA GGTGTATTCT
    GGCACGTTCA GGAATGGCTT GGAAGACGGA TATGGTGAAT ACAGAATCCC AAACAAGGCC
    CTGAACAAAG AAGACCATTA CGTGGGCCAT TGGAAGGAGG GGAAAATGTG TGGGCAAGGA
    GTGTACAGCT ATGCCTCTGG TGAAGTGTTT GAAGGCTGCT TTCAAGATAA CATGCGCCAC
    GGGCACGGTC TCCTCCGCAG TGGAAAACTG ACTTCTTCCT CTCCCAGCAT GTTCATTGGC
    CAGTGGGTAA TGGACAAGAA AGCAGGGTAT GGCGTCTTCG ATGATATCAC CAGGGGAGAA
    AAGTACATGG GAATGTGGCA GGACGATGCA TGCCAAGGGA ATGGGGTGGT GGTCACCCAG
    TTTGGGTTAT ACTACGAAGG CAACTTCCAC CTTAATAAAA TGATGGGAAA TGGGGTTTTG
    CTTTCGGAAG ATGATACTAT ATATGAAGGA GAATTTTCTG ATGACTGGAC ACTTTGTGGA
    AAGGGAACAC TGACTATGCC AAATGGAGAT TATATTGAAG GTTATTTTAG TGGAGAATGG
    GGATCTGGGA TAAAAATCAC TGGGACCTAC TTCAAACCTA GCCTATATGA GAGTGATAAG
    GACAGGCCCA AAGCCTTCAG GAAGCTGGGA AACCTGGCCG TGGCCGCCGA TGAGAAATGG
    AGAGCAGTGT TTGACGAATG CTGGCGCCAG CTGGGCTGTG AGAGCCCAGG CCAAGGGGAG
    GTTTGGAAAG CGTGGGATAA TATTGCTGTG GCCTTGACCA CCAACCGTCG CCAGCATAAA
    GACAGTCCAG AAATACTAAG CCGTTCACAG ACTCAGACCC TGGAGAGTTT GGAGTACATT
    CCCCAGCACG TTGGCGCCTT CTCTGTGGAG AAATATGATG ATATCAAGAA GTATTTAATA
    AAGGCCTGTG ACACTCCTCT GCACCCACTG GGCAGGCTTG TGGAGACCCT GGTTGCAGTG
    TATAGAATGA CTTATGTGGG CGTAGGGGCC AACCGTCGGT TACTGCAGGA AGCTGTGAAG
    GAGATTAAGT CTTATCTCAA GAGGATTTTC CAACTTGTGA GGTTCTTGTT TCCTGAGCTT
    CCTGAAGAGG GCAGCACAGT TCCTCTCTCT GCTCCTCTGC CCACTGGAAG AAGATCCTTC
    TGCACTGGGA AATCAGATTC CAGATCCGAG TCACCAGAGC CAGGTTATGT AGTAACAAGT
    TCTGGCTTAC TGCTTCCGGT GCTGCTGCCT CGGCTCTACC CGCCTCTCTT CATGCTCTAT
    GCCCTGGACA ATGACCGAGA GGAAGACATT TACTGGGAAT GTGTGCTTCG ACTAAACAAG
    CAGCCAGATA TTGCTCTCCT GGGCTTTCTT GGAGTACAGA GGAAATTCTG GCCAGCAACC
    TTGTCAATCC TTGGAGAGAG TAAAAAGGTA CTGCCATCCA CAAAGGATGC TTGTTTTGCA
    TCTGCAGTAG AATGTCTGCA GCAGATCAGC ACAACATTTA CTCCATCAGA CAAACTTAAA
    GTGATCCAGC AGACCTTCGA AGAAATCTCT CAGAGTGTGC TGGCGTCGCT GCAGGAGGAC
    TTCCTTTGGT CCATGGATGA CTTGTTCCCT GTCTTCTTAT ACGTGGTGCT GCGGGCCAGG
    ATTCGGAATT TAGGCTCTGA AGTTCATCTC ATTGAGGATC TGATGGACCC CTATCTCCAG
    CATGGGGAGC AAGGCATAAT GTTCACCACC TTGAAGGCCT GTTACTACCA GATTCAGCGT
    GAGAAGCTTA ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa03946
    Clone ID Related Accession (Same CDS sequence) XM_006245001.3 , NM_001013413.1
    Accession Version NM_001013413.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Rattus norvegicus(Norway rat)
    Product alsin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BK005190.1. On or before Feb 3, 2007 this sequence version replaced XM_343574.3, XM_001061827.1, XM_001064519.1, XM_001064573.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BK005190.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAAAAG CTCAGCAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
    CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGAGGA
    AAAACAGTTC TTCAGGCGGC CCTTGGCGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC CCTTCCCTGG AAAAGTGAAG CAGCAGAAAT TTGTCCAAGC
    AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTG TTACTGTGGC AACAGGGAGC
    TTCCACAGTG GAGCGGTGAC AGAAAGTGGA GTGGTGTACA TGTGGGGGGA GAACGCTGCC
    GGGCAGTGTG CAGTAGCGAA CCAGCAGTAT GTGTCGGAAC CAAGTCCTGT CAGCATTTCT
    GACTCGGAGA CCAGCCCCTT GCTAGCAGTT AGGATTCTGC AGTTGGCATG TGGCGAGGAA
    CACACACTGG CATTGTCAAT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG
    GGTCTCATCA CCACCACCTT CCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGA
    CGAGTGGTGC TCCAGGTGGC CTGTGGTGCG TTCCACAGCC TTGCACTTGT GCAGTGCCTC
    CCTCCCCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC
    ACCATGACAG ACAAAGAGGA CCACGTGATA ATATCAGACA GCCATTGCTG CCCTTTAGGT
    GTGACACTGT CTGAATCTCA AGCAGAAAAG CACGCCAGCA CTGTGACCAG CCCTCACCCG
    GAGACGCTGG ATGGGCAAGG AGAGGTGTTT GAGAACACAG TAGCAGAAGC TGAACTGAAT
    ATGGGAAGCG ATCAGACCAC AAGTGGCAGT GCCATTTCCG CCCAGCAGAA CATCGTGGGA
    ATGGCTGAAG TGTCTTCTGC CAGAACAGCT CCCTCATACC CAGACACCCA GACGGTAACT
    GCATACCTGC AGAAGCTATC AGAGCATTCA GTGAAGGAGA ACCATGAGCG TGAGGAAAAG
    CTACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC
    ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTGAGA
    GTGGCCGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTATAGC
    ACTGCCCCCT GCGAGCCAGG AGCTCCGTCA GGCACTGCCT CCACAGGCCC AGAAAGTCTG
    AAAGATCTCA GAGAAGAGCA GGTGAAACAG GAATCACTAC AAGGGAAGAA AAGCTCAAGC
    CTCATGGATA TCAGAGAGGA GGAGTCGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGA
    TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCCG CCAGGGTGAA AACACGGACA
    GTGGTTCTGA CTCCCACGTA CAGTGGAGAA GCAGATGCCC TCCTGCCTTC GCTGAGAACA
    GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTGG GGCACGGCGA TGTCCTGCCC
    AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTCATCCA CTTGGAGGCA
    GGTGGCTCCC ACTCTCTCGC ACTGACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT
    ACCTTCGGTC AGCTGGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCGAAGGTT
    AGCAGTGAAA GTGGAGTCTG GAGTGTAGCT GCAGGCCACG GTTATTCCCT GTTTTTAGTG
    GACACTGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GCCAGGACCG TGCAGAAGGT
    GACACCCTGC CAGAGAATCC CAGTGGTACA ACGACTCCGG TACTTCTCTC CTGTAGTAAG
    CTTGGATATA TCAGCAGAGT GACAGCAGGA AAAGATAGCT ACCTAGCCTT GGTGGATAAG
    AACATCATGG GATACATCGC CAGCCTCCAT GAGTTGGCTA CCACAGAAAG ACGGTTTTAC
    TCGAAACTAA GTGAAATCAA GTCACAGATA CTTAGGCCTC TTCTCAGTCT GGAACATTTG
    GGCACTGTGA CCACTGTCCA GCTGTTGCAG GAAGTCGCCA GCCGGTTCAG CAAGTTGTGT
    TACCTCATTG GGCAGCATGG AGCCTCACTG AGCAGCTACC TGCAAGGCAT GAAGGAAGCC
    AGGAACCTGG TCATCATGAA GCACTCGAGT CTTTTCTTGG ACAGTTACAC AGAGTATTGT
    ACATCAGTTT CAAATTTTCT GGTTATGGGA GGATCCCAGC TTCTTGCTAA GCCTGCAATT
    GATTTCCTAA ATAAAAACCA AGAACTCTTG CAAGATTTGT CAGAAGTGAA TGATGAGAAC
    ACTCAGTTGA TGGAAATACT GAATGCGCTG TTTTTCTTGC CAATCAGACG ACTTCATAAT
    TATGCAAAAG TTTTGCTAAA GCTCGCCACT TGCTTTGAAG TGACATCACC AGAGTATCAA
    AAGCTGCAGG ATTCCAGTTC TTGTTATGAG TCTCTTGCTC TCCATCTTGG CAAGAAGAGG
    AAGGAAGCAG AGTACACGCT GAGCTTCTGG AAGACCTTCC CTGGAAAAAT GACGGATTCC
    TTGAGGAAGC CAGAGCGCCG GCTGCTGTGT GAGAGCAGTA ACCGAGCCCT CTCCCTGCAG
    CATGCTGGCA GGTTTTCTGT GAATTGGTTC ATTCTCTTCA ATGATGCCCT GGTCCATGCT
    CAGTTCTCCA CACACCACGT GTTCCCTTTG GCCACACTCT GGGCAGAGCC ACTTTCTGAA
    GAAACTGGTG GCGTGAATGG CTTAAAGATA ACCACACCTG AAGAACAGTT CACGCTCATT
    TCTTCAACAC CCCAGGAAAA GACCAAGTGG CTTCGGGCTA TCAGCCAAGC CGTGGACCAG
    GCTTTGAGGG GGACGTCTGA CTTCCCACTT TACGGAGGCA GCAGCACTGT TCAGAGGCAG
    GAACCACCCA TCTCAAGAAG TGCCAAATAC ACTTTCTACA AGGATACTCG CCTAAAGGAC
    GCCACTTACG ATGGGCGCTG GCTTTCAGGG AAGCCGCACG GAAGGGGTGT TTTGAAGTGG
    CCTGATGGGA AGGTGTATTC TGGCACGTTC AGGAATGGCT TGGAAGACGG ATATGGTGAA
    TACAGAATCC CAAACAAGGC CCTGAACAAA GAAGACCATT ACGTGGGCCA TTGGAAGGAG
    GGGAAAATGT GTGGGCAAGG AGTGTACAGC TATGCCTCTG GTGAAGTGTT TGAAGGCTGC
    TTTCAAGATA ACATGCGCCA CGGGCACGGT CTCCTCCGCA GTGGAAAACT GACTTCTTCC
    TCTCCCAGCA TGTTCATTGG CCAGTGGGTA ATGGACAAGA AAGCAGGGTA TGGCGTCTTC
    GATGATATCA CCAGGGGAGA AAAGTACATG GGAATGTGGC AGGACGATGC ATGCCAAGGG
    AATGGGGTGG TGGTCACCCA GTTTGGGTTA TACTACGAAG GCAACTTCCA CCTTAATAAA
    ATGATGGGAA ATGGGGTTTT GCTTTCGGAA GATGATACTA TATATGAAGG AGAATTTTCT
    GATGACTGGA CACTTTGTGG AAAGGGAACA CTGACTATGC CAAATGGAGA TTATATTGAA
    GGTTATTTTA GTGGAGAATG GGGATCTGGG ATAAAAATCA CTGGGACCTA CTTCAAACCT
    AGCCTATATG AGAGTGATAA GGACAGGCCC AAAGCCTTCA GGAAGCTGGG AAACCTGGCC
    GTGGCCGCCG ATGAGAAATG GAGAGCAGTG TTTGACGAAT GCTGGCGCCA GCTGGGCTGT
    GAGAGCCCAG GCCAAGGGGA GGTTTGGAAA GCGTGGGATA ATATTGCTGT GGCCTTGACC
    ACCAACCGTC GCCAGCATAA AGACAGTCCA GAAATACTAA GCCGTTCACA GACTCAGACC
    CTGGAGAGTT TGGAGTACAT TCCCCAGCAC GTTGGCGCCT TCTCTGTGGA GAAATATGAT
    GATATCAAGA AGTATTTAAT AAAGGCCTGT GACACTCCTC TGCACCCACT GGGCAGGCTT
    GTGGAGACCC TGGTTGCAGT GTATAGAATG ACTTATGTGG GCGTAGGGGC CAACCGTCGG
    TTACTGCAGG AAGCTGTGAA GGAGATTAAG TCTTATCTCA AGAGGATTTT CCAACTTGTG
    AGGTTCTTGT TTCCTGAGCT TCCTGAAGAG GGCAGCACAG TTCCTCTCTC TGCTCCTCTG
    CCCACTGGAA GAAGATCCTT CTGCACTGGG AAATCAGATT CCAGATCCGA GTCACCAGAG
    CCAGGTTATG TAGTAACAAG TTCTGGCTTA CTGCTTCCGG TGCTGCTGCC TCGGCTCTAC
    CCGCCTCTCT TCATGCTCTA TGCCCTGGAC AATGACCGAG AGGAAGACAT TTACTGGGAA
    TGTGTGCTTC GACTAAACAA GCAGCCAGAT ATTGCTCTCC TGGGCTTTCT TGGAGTACAG
    AGGAAATTCT GGCCAGCAAC CTTGTCAATC CTTGGAGAGA GTAAAAAGGT ACTGCCATCC
    ACAAAGGATG CTTGTTTTGC ATCTGCAGTA GAATGTCTGC AGCAGATCAG CACAACATTT
    ACTCCATCAG ACAAACTTAA AGTGATCCAG CAGACCTTCG AAGAAATCTC TCAGAGTGTG
    CTGGCGTCGC TGCAGGAGGA CTTCCTTTGG TCCATGGATG ACTTGTTCCC TGTCTTCTTA
    TACGTGGTGC TGCGGGCCAG GATTCGGAAT TTAGGCTCTG AAGTTCATCT CATTGAGGAT
    CTGATGGACC CCTATCTCCA GCATGGGGAG CAAGGCATAA TGTTCACCAC CTTGAAGGCC
    TGTTACTACC AGATTCAGCG TGAGAAGCTT AACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001013431.1
    CDS113..5068
    Translation

    Target ORF information:

    RefSeq Version NM_001013413.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus alsin Rho guanine nucleotide exchange factor ALS2 (Als2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001013413.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAAAAG CTCAGCAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
    CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGAGGA
    AAAACAGTTC TTCAGGCGGC CCTTGGCGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC CCTTCCCTGG AAAAGTGAAG CAGCAGAAAT TTGTCCAAGC
    AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTG TTACTGTGGC AACAGGGAGC
    TTCCACAGTG GAGCGGTGAC AGAAAGTGGA GTGGTGTACA TGTGGGGGGA GAACGCTGCC
    GGGCAGTGTG CAGTAGCGAA CCAGCAGTAT GTGTCGGAAC CAAGTCCTGT CAGCATTTCT
    GACTCGGAGA CCAGCCCCTT GCTAGCAGTT AGGATTCTGC AGTTGGCATG TGGCGAGGAA
    CACACACTGG CATTGTCAAT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG
    GGTCTCATCA CCACCACCTT CCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGA
    CGAGTGGTGC TCCAGGTGGC CTGTGGTGCG TTCCACAGCC TTGCACTTGT GCAGTGCCTC
    CCTCCCCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC
    ACCATGACAG ACAAAGAGGA CCACGTGATA ATATCAGACA GCCATTGCTG CCCTTTAGGT
    GTGACACTGT CTGAATCTCA AGCAGAAAAG CACGCCAGCA CTGTGACCAG CCCTCACCCG
    GAGACGCTGG ATGGGCAAGG AGAGGTGTTT GAGAACACAG TAGCAGAAGC TGAACTGAAT
    ATGGGAAGCG ATCAGACCAC AAGTGGCAGT GCCATTTCCG CCCAGCAGAA CATCGTGGGA
    ATGGCTGAAG TGTCTTCTGC CAGAACAGCT CCCTCATACC CAGACACCCA GACGGTAACT
    GCATACCTGC AGAAGCTATC AGAGCATTCA GTGAAGGAGA ACCATGAGCG TGAGGAAAAG
    CTACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC
    ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTGAGA
    GTGGCCGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTATAGC
    ACTGCCCCCT GCGAGCCAGG AGCTCCGTCA GGCACTGCCT CCACAGGCCC AGAAAGTCTG
    AAAGATCTCA GAGAAGAGCA GGTGAAACAG GAATCACTAC AAGGGAAGAA AAGCTCAAGC
    CTCATGGATA TCAGAGAGGA GGAGTCGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGA
    TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCCG CCAGGGTGAA AACACGGACA
    GTGGTTCTGA CTCCCACGTA CAGTGGAGAA GCAGATGCCC TCCTGCCTTC GCTGAGAACA
    GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTGG GGCACGGCGA TGTCCTGCCC
    AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTCATCCA CTTGGAGGCA
    GGTGGCTCCC ACTCTCTCGC ACTGACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT
    ACCTTCGGTC AGCTGGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCGAAGGTT
    AGCAGTGAAA GTGGAGTCTG GAGTGTAGCT GCAGGCCACG GTTATTCCCT GTTTTTAGTG
    GACACTGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GCCAGGACCG TGCAGAAGGT
    GACACCCTGC CAGAGAATCC CAGTGGTACA ACGACTCCGG TACTTCTCTC CTGTAGTAAG
    CTTGGATATA TCAGCAGAGT GACAGCAGGA AAAGATAGCT ACCTAGCCTT GGTGGATAAG
    AACATCATGG GATACATCGC CAGCCTCCAT GAGTTGGCTA CCACAGAAAG ACGGTTTTAC
    TCGAAACTAA GTGAAATCAA GTCACAGATA CTTAGGCCTC TTCTCAGTCT GGAACATTTG
    GGCACTGTGA CCACTGTCCA GCTGTTGCAG GAAGTCGCCA GCCGGTTCAG CAAGTTGTGT
    TACCTCATTG GGCAGCATGG AGCCTCACTG AGCAGCTACC TGCAAGGCAT GAAGGAAGCC
    AGGAACCTGG TCATCATGAA GCACTCGAGT CTTTTCTTGG ACAGTTACAC AGAGTATTGT
    ACATCAGTTT CAAATTTTCT GGTTATGGGA GGATCCCAGC TTCTTGCTAA GCCTGCAATT
    GATTTCCTAA ATAAAAACCA AGAACTCTTG CAAGATTTGT CAGAAGTGAA TGATGAGAAC
    ACTCAGTTGA TGGAAATACT GAATGCGCTG TTTTTCTTGC CAATCAGACG ACTTCATAAT
    TATGCAAAAG TTTTGCTAAA GCTCGCCACT TGCTTTGAAG TGACATCACC AGAGTATCAA
    AAGCTGCAGG ATTCCAGTTC TTGTTATGAG TCTCTTGCTC TCCATCTTGG CAAGAAGAGG
    AAGGAAGCAG AGTACACGCT GAGCTTCTGG AAGACCTTCC CTGGAAAAAT GACGGATTCC
    TTGAGGAAGC CAGAGCGCCG GCTGCTGTGT GAGAGCAGTA ACCGAGCCCT CTCCCTGCAG
    CATGCTGGCA GGTTTTCTGT GAATTGGTTC ATTCTCTTCA ATGATGCCCT GGTCCATGCT
    CAGTTCTCCA CACACCACGT GTTCCCTTTG GCCACACTCT GGGCAGAGCC ACTTTCTGAA
    GAAACTGGTG GCGTGAATGG CTTAAAGATA ACCACACCTG AAGAACAGTT CACGCTCATT
    TCTTCAACAC CCCAGGAAAA GACCAAGTGG CTTCGGGCTA TCAGCCAAGC CGTGGACCAG
    GCTTTGAGGG GGACGTCTGA CTTCCCACTT TACGGAGGCA GCAGCACTGT TCAGAGGCAG
    GAACCACCCA TCTCAAGAAG TGCCAAATAC ACTTTCTACA AGGATACTCG CCTAAAGGAC
    GCCACTTACG ATGGGCGCTG GCTTTCAGGG AAGCCGCACG GAAGGGGTGT TTTGAAGTGG
    CCTGATGGGA AGGTGTATTC TGGCACGTTC AGGAATGGCT TGGAAGACGG ATATGGTGAA
    TACAGAATCC CAAACAAGGC CCTGAACAAA GAAGACCATT ACGTGGGCCA TTGGAAGGAG
    GGGAAAATGT GTGGGCAAGG AGTGTACAGC TATGCCTCTG GTGAAGTGTT TGAAGGCTGC
    TTTCAAGATA ACATGCGCCA CGGGCACGGT CTCCTCCGCA GTGGAAAACT GACTTCTTCC
    TCTCCCAGCA TGTTCATTGG CCAGTGGGTA ATGGACAAGA AAGCAGGGTA TGGCGTCTTC
    GATGATATCA CCAGGGGAGA AAAGTACATG GGAATGTGGC AGGACGATGC ATGCCAAGGG
    AATGGGGTGG TGGTCACCCA GTTTGGGTTA TACTACGAAG GCAACTTCCA CCTTAATAAA
    ATGATGGGAA ATGGGGTTTT GCTTTCGGAA GATGATACTA TATATGAAGG AGAATTTTCT
    GATGACTGGA CACTTTGTGG AAAGGGAACA CTGACTATGC CAAATGGAGA TTATATTGAA
    GGTTATTTTA GTGGAGAATG GGGATCTGGG ATAAAAATCA CTGGGACCTA CTTCAAACCT
    AGCCTATATG AGAGTGATAA GGACAGGCCC AAAGCCTTCA GGAAGCTGGG AAACCTGGCC
    GTGGCCGCCG ATGAGAAATG GAGAGCAGTG TTTGACGAAT GCTGGCGCCA GCTGGGCTGT
    GAGAGCCCAG GCCAAGGGGA GGTTTGGAAA GCGTGGGATA ATATTGCTGT GGCCTTGACC
    ACCAACCGTC GCCAGCATAA AGACAGTCCA GAAATACTAA GCCGTTCACA GACTCAGACC
    CTGGAGAGTT TGGAGTACAT TCCCCAGCAC GTTGGCGCCT TCTCTGTGGA GAAATATGAT
    GATATCAAGA AGTATTTAAT AAAGGCCTGT GACACTCCTC TGCACCCACT GGGCAGGCTT
    GTGGAGACCC TGGTTGCAGT GTATAGAATG ACTTATGTGG GCGTAGGGGC CAACCGTCGG
    TTACTGCAGG AAGCTGTGAA GGAGATTAAG TCTTATCTCA AGAGGATTTT CCAACTTGTG
    AGGTTCTTGT TTCCTGAGCT TCCTGAAGAG GGCAGCACAG TTCCTCTCTC TGCTCCTCTG
    CCCACTGGAA GAAGATCCTT CTGCACTGGG AAATCAGATT CCAGATCCGA GTCACCAGAG
    CCAGGTTATG TAGTAACAAG TTCTGGCTTA CTGCTTCCGG TGCTGCTGCC TCGGCTCTAC
    CCGCCTCTCT TCATGCTCTA TGCCCTGGAC AATGACCGAG AGGAAGACAT TTACTGGGAA
    TGTGTGCTTC GACTAAACAA GCAGCCAGAT ATTGCTCTCC TGGGCTTTCT TGGAGTACAG
    AGGAAATTCT GGCCAGCAAC CTTGTCAATC CTTGGAGAGA GTAAAAAGGT ACTGCCATCC
    ACAAAGGATG CTTGTTTTGC ATCTGCAGTA GAATGTCTGC AGCAGATCAG CACAACATTT
    ACTCCATCAG ACAAACTTAA AGTGATCCAG CAGACCTTCG AAGAAATCTC TCAGAGTGTG
    CTGGCGTCGC TGCAGGAGGA CTTCCTTTGG TCCATGGATG ACTTGTTCCC TGTCTTCTTA
    TACGTGGTGC TGCGGGCCAG GATTCGGAAT TTAGGCTCTG AAGTTCATCT CATTGAGGAT
    CTGATGGACC CCTATCTCCA GCATGGGGAG CAAGGCATAA TGTTCACCAC CTTGAAGGCC
    TGTTACTACC AGATTCAGCG TGAGAAGCTT AACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa03946
    Clone ID Related Accession (Same CDS sequence) XM_006245001.3 , NM_001013413.1
    Accession Version XM_006245001.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product alsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245001.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAAAAG CTCAGCAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
    CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGAGGA
    AAAACAGTTC TTCAGGCGGC CCTTGGCGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC CCTTCCCTGG AAAAGTGAAG CAGCAGAAAT TTGTCCAAGC
    AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTG TTACTGTGGC AACAGGGAGC
    TTCCACAGTG GAGCGGTGAC AGAAAGTGGA GTGGTGTACA TGTGGGGGGA GAACGCTGCC
    GGGCAGTGTG CAGTAGCGAA CCAGCAGTAT GTGTCGGAAC CAAGTCCTGT CAGCATTTCT
    GACTCGGAGA CCAGCCCCTT GCTAGCAGTT AGGATTCTGC AGTTGGCATG TGGCGAGGAA
    CACACACTGG CATTGTCAAT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG
    GGTCTCATCA CCACCACCTT CCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGA
    CGAGTGGTGC TCCAGGTGGC CTGTGGTGCG TTCCACAGCC TTGCACTTGT GCAGTGCCTC
    CCTCCCCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC
    ACCATGACAG ACAAAGAGGA CCACGTGATA ATATCAGACA GCCATTGCTG CCCTTTAGGT
    GTGACACTGT CTGAATCTCA AGCAGAAAAG CACGCCAGCA CTGTGACCAG CCCTCACCCG
    GAGACGCTGG ATGGGCAAGG AGAGGTGTTT GAGAACACAG TAGCAGAAGC TGAACTGAAT
    ATGGGAAGCG ATCAGACCAC AAGTGGCAGT GCCATTTCCG CCCAGCAGAA CATCGTGGGA
    ATGGCTGAAG TGTCTTCTGC CAGAACAGCT CCCTCATACC CAGACACCCA GACGGTAACT
    GCATACCTGC AGAAGCTATC AGAGCATTCA GTGAAGGAGA ACCATGAGCG TGAGGAAAAG
    CTACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC
    ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTGAGA
    GTGGCCGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTATAGC
    ACTGCCCCCT GCGAGCCAGG AGCTCCGTCA GGCACTGCCT CCACAGGCCC AGAAAGTCTG
    AAAGATCTCA GAGAAGAGCA GGTGAAACAG GAATCACTAC AAGGGAAGAA AAGCTCAAGC
    CTCATGGATA TCAGAGAGGA GGAGTCGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGA
    TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCCG CCAGGGTGAA AACACGGACA
    GTGGTTCTGA CTCCCACGTA CAGTGGAGAA GCAGATGCCC TCCTGCCTTC GCTGAGAACA
    GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTGG GGCACGGCGA TGTCCTGCCC
    AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTCATCCA CTTGGAGGCA
    GGTGGCTCCC ACTCTCTCGC ACTGACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT
    ACCTTCGGTC AGCTGGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCGAAGGTT
    AGCAGTGAAA GTGGAGTCTG GAGTGTAGCT GCAGGCCACG GTTATTCCCT GTTTTTAGTG
    GACACTGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GCCAGGACCG TGCAGAAGGT
    GACACCCTGC CAGAGAATCC CAGTGGTACA ACGACTCCGG TACTTCTCTC CTGTAGTAAG
    CTTGGATATA TCAGCAGAGT GACAGCAGGA AAAGATAGCT ACCTAGCCTT GGTGGATAAG
    AACATCATGG GATACATCGC CAGCCTCCAT GAGTTGGCTA CCACAGAAAG ACGGTTTTAC
    TCGAAACTAA GTGAAATCAA GTCACAGATA CTTAGGCCTC TTCTCAGTCT GGAACATTTG
    GGCACTGTGA CCACTGTCCA GCTGTTGCAG GAAGTCGCCA GCCGGTTCAG CAAGTTGTGT
    TACCTCATTG GGCAGCATGG AGCCTCACTG AGCAGCTACC TGCAAGGCAT GAAGGAAGCC
    AGGAACCTGG TCATCATGAA GCACTCGAGT CTTTTCTTGG ACAGTTACAC AGAGTATTGT
    ACATCAGTTT CAAATTTTCT GGTTATGGGA GGATCCCAGC TTCTTGCTAA GCCTGCAATT
    GATTTCCTAA ATAAAAACCA AGAACTCTTG CAAGATTTGT CAGAAGTGAA TGATGAGAAC
    ACTCAGTTGA TGGAAATACT GAATGCGCTG TTTTTCTTGC CAATCAGACG ACTTCATAAT
    TATGCAAAAG TTTTGCTAAA GCTCGCCACT TGCTTTGAAG TGACATCACC AGAGTATCAA
    AAGCTGCAGG ATTCCAGTTC TTGTTATGAG TCTCTTGCTC TCCATCTTGG CAAGAAGAGG
    AAGGAAGCAG AGTACACGCT GAGCTTCTGG AAGACCTTCC CTGGAAAAAT GACGGATTCC
    TTGAGGAAGC CAGAGCGCCG GCTGCTGTGT GAGAGCAGTA ACCGAGCCCT CTCCCTGCAG
    CATGCTGGCA GGTTTTCTGT GAATTGGTTC ATTCTCTTCA ATGATGCCCT GGTCCATGCT
    CAGTTCTCCA CACACCACGT GTTCCCTTTG GCCACACTCT GGGCAGAGCC ACTTTCTGAA
    GAAACTGGTG GCGTGAATGG CTTAAAGATA ACCACACCTG AAGAACAGTT CACGCTCATT
    TCTTCAACAC CCCAGGAAAA GACCAAGTGG CTTCGGGCTA TCAGCCAAGC CGTGGACCAG
    GCTTTGAGGG GGACGTCTGA CTTCCCACTT TACGGAGGCA GCAGCACTGT TCAGAGGCAG
    GAACCACCCA TCTCAAGAAG TGCCAAATAC ACTTTCTACA AGGATACTCG CCTAAAGGAC
    GCCACTTACG ATGGGCGCTG GCTTTCAGGG AAGCCGCACG GAAGGGGTGT TTTGAAGTGG
    CCTGATGGGA AGGTGTATTC TGGCACGTTC AGGAATGGCT TGGAAGACGG ATATGGTGAA
    TACAGAATCC CAAACAAGGC CCTGAACAAA GAAGACCATT ACGTGGGCCA TTGGAAGGAG
    GGGAAAATGT GTGGGCAAGG AGTGTACAGC TATGCCTCTG GTGAAGTGTT TGAAGGCTGC
    TTTCAAGATA ACATGCGCCA CGGGCACGGT CTCCTCCGCA GTGGAAAACT GACTTCTTCC
    TCTCCCAGCA TGTTCATTGG CCAGTGGGTA ATGGACAAGA AAGCAGGGTA TGGCGTCTTC
    GATGATATCA CCAGGGGAGA AAAGTACATG GGAATGTGGC AGGACGATGC ATGCCAAGGG
    AATGGGGTGG TGGTCACCCA GTTTGGGTTA TACTACGAAG GCAACTTCCA CCTTAATAAA
    ATGATGGGAA ATGGGGTTTT GCTTTCGGAA GATGATACTA TATATGAAGG AGAATTTTCT
    GATGACTGGA CACTTTGTGG AAAGGGAACA CTGACTATGC CAAATGGAGA TTATATTGAA
    GGTTATTTTA GTGGAGAATG GGGATCTGGG ATAAAAATCA CTGGGACCTA CTTCAAACCT
    AGCCTATATG AGAGTGATAA GGACAGGCCC AAAGCCTTCA GGAAGCTGGG AAACCTGGCC
    GTGGCCGCCG ATGAGAAATG GAGAGCAGTG TTTGACGAAT GCTGGCGCCA GCTGGGCTGT
    GAGAGCCCAG GCCAAGGGGA GGTTTGGAAA GCGTGGGATA ATATTGCTGT GGCCTTGACC
    ACCAACCGTC GCCAGCATAA AGACAGTCCA GAAATACTAA GCCGTTCACA GACTCAGACC
    CTGGAGAGTT TGGAGTACAT TCCCCAGCAC GTTGGCGCCT TCTCTGTGGA GAAATATGAT
    GATATCAAGA AGTATTTAAT AAAGGCCTGT GACACTCCTC TGCACCCACT GGGCAGGCTT
    GTGGAGACCC TGGTTGCAGT GTATAGAATG ACTTATGTGG GCGTAGGGGC CAACCGTCGG
    TTACTGCAGG AAGCTGTGAA GGAGATTAAG TCTTATCTCA AGAGGATTTT CCAACTTGTG
    AGGTTCTTGT TTCCTGAGCT TCCTGAAGAG GGCAGCACAG TTCCTCTCTC TGCTCCTCTG
    CCCACTGGAA GAAGATCCTT CTGCACTGGG AAATCAGATT CCAGATCCGA GTCACCAGAG
    CCAGGTTATG TAGTAACAAG TTCTGGCTTA CTGCTTCCGG TGCTGCTGCC TCGGCTCTAC
    CCGCCTCTCT TCATGCTCTA TGCCCTGGAC AATGACCGAG AGGAAGACAT TTACTGGGAA
    TGTGTGCTTC GACTAAACAA GCAGCCAGAT ATTGCTCTCC TGGGCTTTCT TGGAGTACAG
    AGGAAATTCT GGCCAGCAAC CTTGTCAATC CTTGGAGAGA GTAAAAAGGT ACTGCCATCC
    ACAAAGGATG CTTGTTTTGC ATCTGCAGTA GAATGTCTGC AGCAGATCAG CACAACATTT
    ACTCCATCAG ACAAACTTAA AGTGATCCAG CAGACCTTCG AAGAAATCTC TCAGAGTGTG
    CTGGCGTCGC TGCAGGAGGA CTTCCTTTGG TCCATGGATG ACTTGTTCCC TGTCTTCTTA
    TACGTGGTGC TGCGGGCCAG GATTCGGAAT TTAGGCTCTG AAGTTCATCT CATTGAGGAT
    CTGATGGACC CCTATCTCCA GCATGGGGAG CAAGGCATAA TGTTCACCAC CTTGAAGGCC
    TGTTACTACC AGATTCAGCG TGAGAAGCTT AACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006245063.1
    CDS443..5398
    Translation

    Target ORF information:

    RefSeq Version XM_006245001.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006245001.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAAAAG CTCAGCAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
    CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGAGGA
    AAAACAGTTC TTCAGGCGGC CCTTGGCGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC CCTTCCCTGG AAAAGTGAAG CAGCAGAAAT TTGTCCAAGC
    AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTG TTACTGTGGC AACAGGGAGC
    TTCCACAGTG GAGCGGTGAC AGAAAGTGGA GTGGTGTACA TGTGGGGGGA GAACGCTGCC
    GGGCAGTGTG CAGTAGCGAA CCAGCAGTAT GTGTCGGAAC CAAGTCCTGT CAGCATTTCT
    GACTCGGAGA CCAGCCCCTT GCTAGCAGTT AGGATTCTGC AGTTGGCATG TGGCGAGGAA
    CACACACTGG CATTGTCAAT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG
    GGTCTCATCA CCACCACCTT CCCAGTGACA AAGCCACAAA AGGTGGAACA CCTTGCTGGA
    CGAGTGGTGC TCCAGGTGGC CTGTGGTGCG TTCCACAGCC TTGCACTTGT GCAGTGCCTC
    CCTCCCCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC
    ACCATGACAG ACAAAGAGGA CCACGTGATA ATATCAGACA GCCATTGCTG CCCTTTAGGT
    GTGACACTGT CTGAATCTCA AGCAGAAAAG CACGCCAGCA CTGTGACCAG CCCTCACCCG
    GAGACGCTGG ATGGGCAAGG AGAGGTGTTT GAGAACACAG TAGCAGAAGC TGAACTGAAT
    ATGGGAAGCG ATCAGACCAC AAGTGGCAGT GCCATTTCCG CCCAGCAGAA CATCGTGGGA
    ATGGCTGAAG TGTCTTCTGC CAGAACAGCT CCCTCATACC CAGACACCCA GACGGTAACT
    GCATACCTGC AGAAGCTATC AGAGCATTCA GTGAAGGAGA ACCATGAGCG TGAGGAAAAG
    CTACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC
    ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTGAGA
    GTGGCCGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTATAGC
    ACTGCCCCCT GCGAGCCAGG AGCTCCGTCA GGCACTGCCT CCACAGGCCC AGAAAGTCTG
    AAAGATCTCA GAGAAGAGCA GGTGAAACAG GAATCACTAC AAGGGAAGAA AAGCTCAAGC
    CTCATGGATA TCAGAGAGGA GGAGTCGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGA
    TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCCG CCAGGGTGAA AACACGGACA
    GTGGTTCTGA CTCCCACGTA CAGTGGAGAA GCAGATGCCC TCCTGCCTTC GCTGAGAACA
    GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTGG GGCACGGCGA TGTCCTGCCC
    AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTCATCCA CTTGGAGGCA
    GGTGGCTCCC ACTCTCTCGC ACTGACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT
    ACCTTCGGTC AGCTGGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCGAAGGTT
    AGCAGTGAAA GTGGAGTCTG GAGTGTAGCT GCAGGCCACG GTTATTCCCT GTTTTTAGTG
    GACACTGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GCCAGGACCG TGCAGAAGGT
    GACACCCTGC CAGAGAATCC CAGTGGTACA ACGACTCCGG TACTTCTCTC CTGTAGTAAG
    CTTGGATATA TCAGCAGAGT GACAGCAGGA AAAGATAGCT ACCTAGCCTT GGTGGATAAG
    AACATCATGG GATACATCGC CAGCCTCCAT GAGTTGGCTA CCACAGAAAG ACGGTTTTAC
    TCGAAACTAA GTGAAATCAA GTCACAGATA CTTAGGCCTC TTCTCAGTCT GGAACATTTG
    GGCACTGTGA CCACTGTCCA GCTGTTGCAG GAAGTCGCCA GCCGGTTCAG CAAGTTGTGT
    TACCTCATTG GGCAGCATGG AGCCTCACTG AGCAGCTACC TGCAAGGCAT GAAGGAAGCC
    AGGAACCTGG TCATCATGAA GCACTCGAGT CTTTTCTTGG ACAGTTACAC AGAGTATTGT
    ACATCAGTTT CAAATTTTCT GGTTATGGGA GGATCCCAGC TTCTTGCTAA GCCTGCAATT
    GATTTCCTAA ATAAAAACCA AGAACTCTTG CAAGATTTGT CAGAAGTGAA TGATGAGAAC
    ACTCAGTTGA TGGAAATACT GAATGCGCTG TTTTTCTTGC CAATCAGACG ACTTCATAAT
    TATGCAAAAG TTTTGCTAAA GCTCGCCACT TGCTTTGAAG TGACATCACC AGAGTATCAA
    AAGCTGCAGG ATTCCAGTTC TTGTTATGAG TCTCTTGCTC TCCATCTTGG CAAGAAGAGG
    AAGGAAGCAG AGTACACGCT GAGCTTCTGG AAGACCTTCC CTGGAAAAAT GACGGATTCC
    TTGAGGAAGC CAGAGCGCCG GCTGCTGTGT GAGAGCAGTA ACCGAGCCCT CTCCCTGCAG
    CATGCTGGCA GGTTTTCTGT GAATTGGTTC ATTCTCTTCA ATGATGCCCT GGTCCATGCT
    CAGTTCTCCA CACACCACGT GTTCCCTTTG GCCACACTCT GGGCAGAGCC ACTTTCTGAA
    GAAACTGGTG GCGTGAATGG CTTAAAGATA ACCACACCTG AAGAACAGTT CACGCTCATT
    TCTTCAACAC CCCAGGAAAA GACCAAGTGG CTTCGGGCTA TCAGCCAAGC CGTGGACCAG
    GCTTTGAGGG GGACGTCTGA CTTCCCACTT TACGGAGGCA GCAGCACTGT TCAGAGGCAG
    GAACCACCCA TCTCAAGAAG TGCCAAATAC ACTTTCTACA AGGATACTCG CCTAAAGGAC
    GCCACTTACG ATGGGCGCTG GCTTTCAGGG AAGCCGCACG GAAGGGGTGT TTTGAAGTGG
    CCTGATGGGA AGGTGTATTC TGGCACGTTC AGGAATGGCT TGGAAGACGG ATATGGTGAA
    TACAGAATCC CAAACAAGGC CCTGAACAAA GAAGACCATT ACGTGGGCCA TTGGAAGGAG
    GGGAAAATGT GTGGGCAAGG AGTGTACAGC TATGCCTCTG GTGAAGTGTT TGAAGGCTGC
    TTTCAAGATA ACATGCGCCA CGGGCACGGT CTCCTCCGCA GTGGAAAACT GACTTCTTCC
    TCTCCCAGCA TGTTCATTGG CCAGTGGGTA ATGGACAAGA AAGCAGGGTA TGGCGTCTTC
    GATGATATCA CCAGGGGAGA AAAGTACATG GGAATGTGGC AGGACGATGC ATGCCAAGGG
    AATGGGGTGG TGGTCACCCA GTTTGGGTTA TACTACGAAG GCAACTTCCA CCTTAATAAA
    ATGATGGGAA ATGGGGTTTT GCTTTCGGAA GATGATACTA TATATGAAGG AGAATTTTCT
    GATGACTGGA CACTTTGTGG AAAGGGAACA CTGACTATGC CAAATGGAGA TTATATTGAA
    GGTTATTTTA GTGGAGAATG GGGATCTGGG ATAAAAATCA CTGGGACCTA CTTCAAACCT
    AGCCTATATG AGAGTGATAA GGACAGGCCC AAAGCCTTCA GGAAGCTGGG AAACCTGGCC
    GTGGCCGCCG ATGAGAAATG GAGAGCAGTG TTTGACGAAT GCTGGCGCCA GCTGGGCTGT
    GAGAGCCCAG GCCAAGGGGA GGTTTGGAAA GCGTGGGATA ATATTGCTGT GGCCTTGACC
    ACCAACCGTC GCCAGCATAA AGACAGTCCA GAAATACTAA GCCGTTCACA GACTCAGACC
    CTGGAGAGTT TGGAGTACAT TCCCCAGCAC GTTGGCGCCT TCTCTGTGGA GAAATATGAT
    GATATCAAGA AGTATTTAAT AAAGGCCTGT GACACTCCTC TGCACCCACT GGGCAGGCTT
    GTGGAGACCC TGGTTGCAGT GTATAGAATG ACTTATGTGG GCGTAGGGGC CAACCGTCGG
    TTACTGCAGG AAGCTGTGAA GGAGATTAAG TCTTATCTCA AGAGGATTTT CCAACTTGTG
    AGGTTCTTGT TTCCTGAGCT TCCTGAAGAG GGCAGCACAG TTCCTCTCTC TGCTCCTCTG
    CCCACTGGAA GAAGATCCTT CTGCACTGGG AAATCAGATT CCAGATCCGA GTCACCAGAG
    CCAGGTTATG TAGTAACAAG TTCTGGCTTA CTGCTTCCGG TGCTGCTGCC TCGGCTCTAC
    CCGCCTCTCT TCATGCTCTA TGCCCTGGAC AATGACCGAG AGGAAGACAT TTACTGGGAA
    TGTGTGCTTC GACTAAACAA GCAGCCAGAT ATTGCTCTCC TGGGCTTTCT TGGAGTACAG
    AGGAAATTCT GGCCAGCAAC CTTGTCAATC CTTGGAGAGA GTAAAAAGGT ACTGCCATCC
    ACAAAGGATG CTTGTTTTGC ATCTGCAGTA GAATGTCTGC AGCAGATCAG CACAACATTT
    ACTCCATCAG ACAAACTTAA AGTGATCCAG CAGACCTTCG AAGAAATCTC TCAGAGTGTG
    CTGGCGTCGC TGCAGGAGGA CTTCCTTTGG TCCATGGATG ACTTGTTCCC TGTCTTCTTA
    TACGTGGTGC TGCGGGCCAG GATTCGGAAT TTAGGCTCTG AAGTTCATCT CATTGAGGAT
    CTGATGGACC CCTATCTCCA GCATGGGGAG CAAGGCATAA TGTTCACCAC CTTGAAGGCC
    TGTTACTACC AGATTCAGCG TGAGAAGCTT AACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.