ALS2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ALS2
Entrez Gene ID	100856109
Full Name	ALS2, alsin Rho guanine nucleotide exchange factor
General protein information	Preferred Names ALS2, alsin Rho guanine nucleotide exchange factor Names alsin amyotrophic lateral sclerosis 2 (juvenile)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022414976.1	XP_022270684.1	alsin isoform X1
XM_022414977.1	XP_022270685.1	alsin isoform X1
XM_003640186.4	XP_003640234.1	alsin isoform X3
XM_014111067.1	XP_013966542.1	alsin isoform X2
XM_003640186.3	XP_003640234.1	alsin isoform X1
XM_005640545.2	XP_005640602.1	alsin isoform X1
XM_022414978.1	XP_022270686.1	alsin isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ALS2	XP_003640234.1
Danio rerio (zebrafish)	als2b	XP_002662342.2
Homo sapiens (human)	ALS2	NP_065970.2
Bos taurus (cattle)	ALS2	XP_005202742.1
Rattus norvegicus (Norway rat)	Als2	NP_001013431.1
Pan troglodytes (chimpanzee)	ALS2	NP_001073389.1
Xenopus tropicalis (tropical clawed frog)	als2	XP_004917813.1
	ALS2	XP_001091562.2
Mus musculus (house mouse)	Als2	NP_001153420.2
Gallus gallus (chicken)	ALS2	XP_421940.2

Gene Ontology

The following ALS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe284800	XM_022414976.1 Latest version!	Canis lupus familiaris ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe284800	XM_022414977.1 Latest version!	Canis lupus familiaris ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe284801	XM_022414978.1 Latest version!	Canis lupus familiaris ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe45243	XM_014111067.1 Latest version!	Canis lupus familiaris amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe31363	XM_003640186.4 Latest version!	Canis lupus familiaris ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe31363	XM_005640545.2 Latest version!	Canis lupus familiaris amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OCe31363	XM_003640186.3	Canis lupus familiaris amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALS2 ( XM_022414976.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022270684.1 Canis lupus familiaris ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X1, mRNA.

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CloneID	OCe284800
Clone ID Related Accession (Same CDS sequence)	XM_022414976.1 , XM_022414977.1
Accession Version	XM_022414976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006619.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AAGCAGGATC CTATTCTGTA ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCAGGCAGC CCTTGGAGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGAAC CGGCAGAGAT TTGTCCGAGT 
TGCCCTATTC TAGAAAATGC CCTGATAGGG CAATTTGTCG TTACTGTGGC ATCAGGGAGT 
TTCCACAATG GAGCAGTAAC AGAAAGTGGT GTAGCATACA TGTGGGGGGA GAACTCTGCA 
GGCCAGTGTG CAGTAGCTAA CCAGCAGTAT GTTCCGGAAC CAAATCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTTCTTT GTTAGCAGTC AGGATTTTGC AGTTGGCATG TGGTGAGGAG 
CACACACTGG CATTATCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG 
GGTCTCATTA CCACTACCTT CCCGGTGACA AAGCCACAAA AGGTGGAACA TCTTGCTGGG 
CGAGTAGTGC TACAAGTTGC CTGTGGTGCG TTCCACAGCT TAGCACTTGT ACAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA AGATGCAACC AGTGCAGCCA ACTCTTGATT 
ACCATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCACTGTTG CCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAAAC CAGGCCAGCA CTGCCATCAG CCCCTCCACT 
GAAACCCTTG ACAATCAAGA AGTATTCGAG AACACGCTTG TAGAAAATGA GCTGCCTGTT 
GCTACTGATA CAAACGTTGG AAATGTTCAG ACCACGGGTG GTGATGCCAT ATCCTCCCAA 
CAAGAAATCA TGGGAACAAC TGAAATTCCT TCTGCCCAAA ATATACCATC ATACCCTGAT 
ACTCAGGCAG TAAATGAGTA CATGCAAAAA CTGTCAGATC ATTCAGTAAG AGACAACTCA 
GAGAACGGCA AAAAGCCAAT GCCATCTCAG GTACCTGCTA AATTTTATAA TATAAAGCCT 
CTTGTAGAAG AAGCAGTTCC TAATCTTCAC AGTCCACCAA CCACAAGCAC CTCAGCCCTA 
AACAGCCTAG TAGTCTCTTG TGCATCTGCT GTTGGTGTGA GAGTGGCTGC TACATATGAA 
GCTGGGGCCT TGTCTCTTAA GAAAGTTATG AACTTTTATA GTACGGCCCC TTGTGAACCT 
GGAACTCAGG CAGGCAGTAG TTCCATAGGC CCAGAAGGTC TGAAAGATAG CAGAGAAGAA 
CAGGTTAAAC AGGAATCAAT GCAAGGAAAG AAAAGTTCAA GTCTCGTGGA TATCCGAGAA 
GAGGAATCAG AGGGAGGCAG CCGAAGACTC TCCCTCCCTG GATTGTTGTC CCAAGTTTCC 
CCCAGGCTCT TAAGAAAAGC TGCTCGGGTA AAAACTCGGA CAGTGGTTCT GACACCCACA 
TACAGCGGAG AAGCAGATGC ACTCCTGCCT TCTCTTAGAA CAGAAGTGTG GACCTGGGGG 
AAAGGGAAGG AAGGACAGCT GGGTCATGGT GATGTTCTGC CAAGGCTTCA ACCACTGTGT 
GTAAAATGTC TGGATGGTAA AGAAGTAATC TATCTGGAGG CAGGTGGTTA CCATTCTCTT 
GCACTTACTG CGAAATCCCA GGTTTACTCA TGGGGTAGCA ATACCTTTGG TCAACTTGGA 
CATTATGATT TTCCAACAAC AGTTCCTCGT CTTGCAAAGG TGAGCAGTGA AAATGGAGTC 
TGGAGTGTCG CTGCCGGCCA GGATTATTCC CTGTTCTTAG TGGATACAGA AGACTTCCAG 
CCTGGGTTGT ATTACAGTGG CCGACAGGAC CCTGCGGAAG GTGACAACCT TCCAGAGAAT 
CACAGTGGCA CTAAGACTCC AGTACTTCTC TCCTGTAGTA AGCTTGGATA TATAAGTAGA 
GTGACAGCAG GAAAAGATAG CTATCTAGCT TTGGTGGACA AGAATGTTAT GGGATATATT 
GCCAGTCTCC ATGAGTTGGC TACCACGGAA AGACGGTTCT ATGCAAAACT AAGTGACATC 
AAATCTCAGA TTCTCAGGCC TCTTCTCAGT TTAGAAAATT TGGGTACTGC AAGCACAGTC 
CAGCTGTTGC AGGAGGTGGC AAGCCGATTC AGCAAGCTGT GTTACCTAAT TGGTCAGCAT 
GGAGCCTCAC TGAGCAGCTT CCTTCATGGG ATAAAGGAAG CCAGGAGTTT GGTCATTCTG 
AAGCATGCAA GTCTCTTCTT GGATAGTTAT ACAGAGTATT GCACATCTAT TACAAATTTC 
CTGGTTATGG GAGGATTCCA ACTTCTTGCT AAGCCTGCCA TTGATTTCCT AAATAAAAAC 
CAGGAGCTAT TACAAGATTT ATCAGAAGTA AATGATGAAA ACACCCAATT GATGGAAATA 
CTGAATACTC TGTTTTTCTT GCCAATCAGA CGACTTCATA ATTATGCAAA AGTTTTGCTA 
AAGCTTGCTA CTTGTTTTGA AGTGACATCT CCAGAATACC AGAAACTGCA GGATTCCAGT 
TCTTGTTATG AGTGTCTTGC TCTCCATCTC GGCAGGAAAA GGAAGGAAGC TGAATACACC 
CTGGGCTTCT GGAAGACCTT TCCTGGGAAA ATGACGGATT CCTTAAGGAA GCCAGAGCGT 
CGACTGCTCT GTGAAAGCAG TAACCGGGCC CTATCTCTGC AGCATGCTGG GAGGTTTTCT 
GTGAATTGGT TCATTCTCTT TAATGATGCC CTGGTCCATG CCCAGTTCTC CACACATCAT 
GTTTTCCCTT TGGCCACACT ATGGGCAGAG CCACTTTCTG AAGAAGCTGG TGGTGTGAAT 
GGCTTAAAGA TAACTACACC TGAGGAGCAG TTCACTCTCA TTTCATCAAC ACCCCAGGAA 
AAGACTAAGT GGCTTCGGGC TATAAGCCAA GCTGTGGATC AGGCTTTGAG GGGGACAGCT 
GATCTTCCAC CTTATGGAAG TGGCAGCAGT ATTCAGAGGC AGGAACCACC CATTTCACGC 
AGTGCCAAGT ATACTTTCTA TAAGGATCCT CGCCTAAAAG ATGCAACCTA TGATGGACGC 
TGGCTTTCAG GGAAGCCTCA TGGCAGAGGG GTTTTGAAAT GGCCTGATGG AAAGATGTAT 
TCTGGCATGT TCAGGAATGG CTTGGAAGAT GGATATGGAG AATACAGAAT CCCAAACAAA 
GCATTGAACA AAGAAGACCA TTATGTAGGC CATTGGAAAG AAGGAAAAAT GTGCGGTCAA 
GGAGTCTATA GTTATGCCTC TGGTGAAGTG TTTGAAGGCT GTTTTCAAGA TAACATGCGT 
CATGGTCATG GTCTCCTTCG AAGCGGAAAA TTAACTTCCT CTTCTCCCAG TATGTTCATT 
GGCCAGTGGG TAATGGATAA GAAAGCAGGA TATGGTGTCT TTGATGATAT CACTAGAGGG 
GAAAAGTATA TGGGAATGTG GCAAGATGAC GTATGTCAAG GGAATGGTGT GGTAGTTACC 
CAATTTGGAT TATACTATGA AGGCAACTTT CACCTTAATA AAATGATGGG AAATGGTGTT 
TTACTTTCTG AAGATGATAC TATATATGAG GGAGAATTTT CAGATGACTG GACTCTTAGT 
GGAAAGGGAA CACTGACTAT GCCAAATGGA GATTACATTG AAGGTTATTT TAGTGGAGAA 
TGGGGATCTG GTATAAAAAT CACAGGAACC TACTTCAAGC CTAGTCTGTA TGAGAGTGAT 
AAAGACAGAC CCAAAGTTTT CAGGAAGCTG GGAAACCTGG CAGTGTCTGC TGATGAAAAG 
TGGAAAGCAG TATTTGATGA ATGTTGGCAT CAACTGGGCT GTGAGAACCC AGGCCAAGGG 
GAAGTTTGGA AAGCATGGGA CAATATTGCT GTGGCCCTGA CCACCAATCG GCGCCAACAC 
AGAGACAGTC CAGAGACATT AAGTCGTTCA CAGACTCAGA CACTAGAGAG TTTGGAATTC 
ATTCCTCAGC ATGTTGGTGC TTTCTCGGTA GAGAAATATG ATGACATCAA GAAATATTTA 
ATAAAGGCAT GTGACACTCC TCTGCACCCC CTGGGCAGGC TTGTGGAGAC ACTGGTTGCA 
GTTTATAGAA TGACATATGT GGGTGTAGGA GCCAATCGCC GGCTACTGCA GGAGGCTGTG 
AAAGAGATTA AATCTTACCT TAAGCGAATT TTCCAGCTGG TGAGATTCTT ATTTCCTGAG 
CTTCCTGAAG AGGGCAGCAC AATTCCTCTT TCTGCTCCTC TGCCAACGGA AAGGAAATCC 
TTTTGCACTG GGAAGTCAGA TTCCAGATCC GAATCACCAG AGCCAGGTTA TGTAGTAACA 
AGTTCTGGGT TACTGCTTCC TGTACTACTA CCTCGGCTCT ACCCACCACT GTTTATGCTC 
TATGCCCTGG ATAATGATCG TGAGGAAGAC ATTTACTGGG AGTGTGTTCT ACGACTAAAT 
AAGCAGCCAG ATATTGCTCT TCTCGGCTTC CTTGGGGTGC AGAGGAAATT TTGGCCAGTG 
ACCTTGTCAA TCCTTGGAGA GAGTAAAAAG GTTCTGCCAA CCACAAAAGA TGCTTGTTTT 
GCCTCAGCTG TAGAATGTCT ACAGCAGATC AGCACAACAT TTACCCCATC AGACAAACTT 
AAGGTCATCC AGCAGACTTT TGAAGAGATC TCTCAGAGTG TCCTGGCATC ACTTCAGGAA 
GACTTCCTGT GGTCCATGGA TGACTTGTTT CCTGTTTTCT TATATGTGGT GCTACGGGCC 
AGGATTAGGA ATTTAGGCTC TGAGGTACAC CTCATTGAAG ATCTAATGGA TCCATATTTA 
CAGCATGGGG AACAGGGTAT AATGTTCACC ACCTTAAAGG CATGTTACTA CCAGATCCAG 
CGTGAGAAGC TTAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022270684.1
CDS	213..5210
Translation	MDSKKRSSTEAEGSKERGLVHVWQAGSYSVTPERLPGWGGKTVLQAALGVKHGVLLTEDGEVYSFGTLPWRSEPAEICPSCPILENALIGQFVVTVASGSFHNGAVTESGVAYMWGENSAGQCAVANQQYVPEPNPVSISDSETSSLLAVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAENQASTAISPSTETLDNQEVFENTLVENELPVATDTNVGNVQTTGGDAISSQQEIMGTTEIPSAQNIPSYPDTQAVNEYMQKLSDHSVRDNSENGKKPMPSQVPAKFYNIKPLVEEAVPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSTAPCEPGTQAGSSSIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIYLEAGGYHSLALTAKSQVYSWGSNTFGQLGHYDFPTTVPRLAKVSSENGVWSVAAGQDYSLFLVDTEDFQPGLYYSGRQDPAEGDNLPENHSGTKTPVLLSCSKLGYISRVTAGKDSYLALVDKNVMGYIASLHELATTERRFYAKLSDIKSQILRPLLSLENLGTASTVQLLQEVASRFSKLCYLIGQHGASLSSFLHGIKEARSLVILKHASLFLDSYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVNDENTQLMEILNTLFFLPIRRLHNYAKVLLKLATCFEVTSPEYQKLQDSSSCYECLALHLGRKRKEAEYTLGFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGTADLPPYGSGSSIQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGMWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKVFRKLGNLAVSADEKWKAVFDECWHQLGCENPGQGEVWKAWDNIAVALTTNRRQHRDSPETLSRSQTQTLESLEFIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALDNDREEDIYWECVLRLNKQPDIALLGFLGVQRKFWPVTLSILGESKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_022414976.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022414976.1

ORF Insert Sequence:

ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AAGCAGGATC CTATTCTGTA ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCAGGCAGC CCTTGGAGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGAAC CGGCAGAGAT TTGTCCGAGT 
TGCCCTATTC TAGAAAATGC CCTGATAGGG CAATTTGTCG TTACTGTGGC ATCAGGGAGT 
TTCCACAATG GAGCAGTAAC AGAAAGTGGT GTAGCATACA TGTGGGGGGA GAACTCTGCA 
GGCCAGTGTG CAGTAGCTAA CCAGCAGTAT GTTCCGGAAC CAAATCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTTCTTT GTTAGCAGTC AGGATTTTGC AGTTGGCATG TGGTGAGGAG 
CACACACTGG CATTATCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG 
GGTCTCATTA CCACTACCTT CCCGGTGACA AAGCCACAAA AGGTGGAACA TCTTGCTGGG 
CGAGTAGTGC TACAAGTTGC CTGTGGTGCG TTCCACAGCT TAGCACTTGT ACAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA AGATGCAACC AGTGCAGCCA ACTCTTGATT 
ACCATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCACTGTTG CCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAAAC CAGGCCAGCA CTGCCATCAG CCCCTCCACT 
GAAACCCTTG ACAATCAAGA AGTATTCGAG AACACGCTTG TAGAAAATGA GCTGCCTGTT 
GCTACTGATA CAAACGTTGG AAATGTTCAG ACCACGGGTG GTGATGCCAT ATCCTCCCAA 
CAAGAAATCA TGGGAACAAC TGAAATTCCT TCTGCCCAAA ATATACCATC ATACCCTGAT 
ACTCAGGCAG TAAATGAGTA CATGCAAAAA CTGTCAGATC ATTCAGTAAG AGACAACTCA 
GAGAACGGCA AAAAGCCAAT GCCATCTCAG GTACCTGCTA AATTTTATAA TATAAAGCCT 
CTTGTAGAAG AAGCAGTTCC TAATCTTCAC AGTCCACCAA CCACAAGCAC CTCAGCCCTA 
AACAGCCTAG TAGTCTCTTG TGCATCTGCT GTTGGTGTGA GAGTGGCTGC TACATATGAA 
GCTGGGGCCT TGTCTCTTAA GAAAGTTATG AACTTTTATA GTACGGCCCC TTGTGAACCT 
GGAACTCAGG CAGGCAGTAG TTCCATAGGC CCAGAAGGTC TGAAAGATAG CAGAGAAGAA 
CAGGTTAAAC AGGAATCAAT GCAAGGAAAG AAAAGTTCAA GTCTCGTGGA TATCCGAGAA 
GAGGAATCAG AGGGAGGCAG CCGAAGACTC TCCCTCCCTG GATTGTTGTC CCAAGTTTCC 
CCCAGGCTCT TAAGAAAAGC TGCTCGGGTA AAAACTCGGA CAGTGGTTCT GACACCCACA 
TACAGCGGAG AAGCAGATGC ACTCCTGCCT TCTCTTAGAA CAGAAGTGTG GACCTGGGGG 
AAAGGGAAGG AAGGACAGCT GGGTCATGGT GATGTTCTGC CAAGGCTTCA ACCACTGTGT 
GTAAAATGTC TGGATGGTAA AGAAGTAATC TATCTGGAGG CAGGTGGTTA CCATTCTCTT 
GCACTTACTG CGAAATCCCA GGTTTACTCA TGGGGTAGCA ATACCTTTGG TCAACTTGGA 
CATTATGATT TTCCAACAAC AGTTCCTCGT CTTGCAAAGG TGAGCAGTGA AAATGGAGTC 
TGGAGTGTCG CTGCCGGCCA GGATTATTCC CTGTTCTTAG TGGATACAGA AGACTTCCAG 
CCTGGGTTGT ATTACAGTGG CCGACAGGAC CCTGCGGAAG GTGACAACCT TCCAGAGAAT 
CACAGTGGCA CTAAGACTCC AGTACTTCTC TCCTGTAGTA AGCTTGGATA TATAAGTAGA 
GTGACAGCAG GAAAAGATAG CTATCTAGCT TTGGTGGACA AGAATGTTAT GGGATATATT 
GCCAGTCTCC ATGAGTTGGC TACCACGGAA AGACGGTTCT ATGCAAAACT AAGTGACATC 
AAATCTCAGA TTCTCAGGCC TCTTCTCAGT TTAGAAAATT TGGGTACTGC AAGCACAGTC 
CAGCTGTTGC AGGAGGTGGC AAGCCGATTC AGCAAGCTGT GTTACCTAAT TGGTCAGCAT 
GGAGCCTCAC TGAGCAGCTT CCTTCATGGG ATAAAGGAAG CCAGGAGTTT GGTCATTCTG 
AAGCATGCAA GTCTCTTCTT GGATAGTTAT ACAGAGTATT GCACATCTAT TACAAATTTC 
CTGGTTATGG GAGGATTCCA ACTTCTTGCT AAGCCTGCCA TTGATTTCCT AAATAAAAAC 
CAGGAGCTAT TACAAGATTT ATCAGAAGTA AATGATGAAA ACACCCAATT GATGGAAATA 
CTGAATACTC TGTTTTTCTT GCCAATCAGA CGACTTCATA ATTATGCAAA AGTTTTGCTA 
AAGCTTGCTA CTTGTTTTGA AGTGACATCT CCAGAATACC AGAAACTGCA GGATTCCAGT 
TCTTGTTATG AGTGTCTTGC TCTCCATCTC GGCAGGAAAA GGAAGGAAGC TGAATACACC 
CTGGGCTTCT GGAAGACCTT TCCTGGGAAA ATGACGGATT CCTTAAGGAA GCCAGAGCGT 
CGACTGCTCT GTGAAAGCAG TAACCGGGCC CTATCTCTGC AGCATGCTGG GAGGTTTTCT 
GTGAATTGGT TCATTCTCTT TAATGATGCC CTGGTCCATG CCCAGTTCTC CACACATCAT 
GTTTTCCCTT TGGCCACACT ATGGGCAGAG CCACTTTCTG AAGAAGCTGG TGGTGTGAAT 
GGCTTAAAGA TAACTACACC TGAGGAGCAG TTCACTCTCA TTTCATCAAC ACCCCAGGAA 
AAGACTAAGT GGCTTCGGGC TATAAGCCAA GCTGTGGATC AGGCTTTGAG GGGGACAGCT 
GATCTTCCAC CTTATGGAAG TGGCAGCAGT ATTCAGAGGC AGGAACCACC CATTTCACGC 
AGTGCCAAGT ATACTTTCTA TAAGGATCCT CGCCTAAAAG ATGCAACCTA TGATGGACGC 
TGGCTTTCAG GGAAGCCTCA TGGCAGAGGG GTTTTGAAAT GGCCTGATGG AAAGATGTAT 
TCTGGCATGT TCAGGAATGG CTTGGAAGAT GGATATGGAG AATACAGAAT CCCAAACAAA 
GCATTGAACA AAGAAGACCA TTATGTAGGC CATTGGAAAG AAGGAAAAAT GTGCGGTCAA 
GGAGTCTATA GTTATGCCTC TGGTGAAGTG TTTGAAGGCT GTTTTCAAGA TAACATGCGT 
CATGGTCATG GTCTCCTTCG AAGCGGAAAA TTAACTTCCT CTTCTCCCAG TATGTTCATT 
GGCCAGTGGG TAATGGATAA GAAAGCAGGA TATGGTGTCT TTGATGATAT CACTAGAGGG 
GAAAAGTATA TGGGAATGTG GCAAGATGAC GTATGTCAAG GGAATGGTGT GGTAGTTACC 
CAATTTGGAT TATACTATGA AGGCAACTTT CACCTTAATA AAATGATGGG AAATGGTGTT 
TTACTTTCTG AAGATGATAC TATATATGAG GGAGAATTTT CAGATGACTG GACTCTTAGT 
GGAAAGGGAA CACTGACTAT GCCAAATGGA GATTACATTG AAGGTTATTT TAGTGGAGAA 
TGGGGATCTG GTATAAAAAT CACAGGAACC TACTTCAAGC CTAGTCTGTA TGAGAGTGAT 
AAAGACAGAC CCAAAGTTTT CAGGAAGCTG GGAAACCTGG CAGTGTCTGC TGATGAAAAG 
TGGAAAGCAG TATTTGATGA ATGTTGGCAT CAACTGGGCT GTGAGAACCC AGGCCAAGGG 
GAAGTTTGGA AAGCATGGGA CAATATTGCT GTGGCCCTGA CCACCAATCG GCGCCAACAC 
AGAGACAGTC CAGAGACATT AAGTCGTTCA CAGACTCAGA CACTAGAGAG TTTGGAATTC 
ATTCCTCAGC ATGTTGGTGC TTTCTCGGTA GAGAAATATG ATGACATCAA GAAATATTTA 
ATAAAGGCAT GTGACACTCC TCTGCACCCC CTGGGCAGGC TTGTGGAGAC ACTGGTTGCA 
GTTTATAGAA TGACATATGT GGGTGTAGGA GCCAATCGCC GGCTACTGCA GGAGGCTGTG 
AAAGAGATTA AATCTTACCT TAAGCGAATT TTCCAGCTGG TGAGATTCTT ATTTCCTGAG 
CTTCCTGAAG AGGGCAGCAC AATTCCTCTT TCTGCTCCTC TGCCAACGGA AAGGAAATCC 
TTTTGCACTG GGAAGTCAGA TTCCAGATCC GAATCACCAG AGCCAGGTTA TGTAGTAACA 
AGTTCTGGGT TACTGCTTCC TGTACTACTA CCTCGGCTCT ACCCACCACT GTTTATGCTC 
TATGCCCTGG ATAATGATCG TGAGGAAGAC ATTTACTGGG AGTGTGTTCT ACGACTAAAT 
AAGCAGCCAG ATATTGCTCT TCTCGGCTTC CTTGGGGTGC AGAGGAAATT TTGGCCAGTG 
ACCTTGTCAA TCCTTGGAGA GAGTAAAAAG GTTCTGCCAA CCACAAAAGA TGCTTGTTTT 
GCCTCAGCTG TAGAATGTCT ACAGCAGATC AGCACAACAT TTACCCCATC AGACAAACTT 
AAGGTCATCC AGCAGACTTT TGAAGAGATC TCTCAGAGTG TCCTGGCATC ACTTCAGGAA 
GACTTCCTGT GGTCCATGGA TGACTTGTTT CCTGTTTTCT TATATGTGGT GCTACGGGCC 
AGGATTAGGA ATTTAGGCTC TGAGGTACAC CTCATTGAAG ATCTAATGGA TCCATATTTA 
CAGCATGGGG AACAGGGTAT AATGTTCACC ACCTTAAAGG CATGTTACTA CCAGATCCAG 
CGTGAGAAGC TTAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_022414977.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022270685.1 Canis lupus familiaris ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X2, mRNA.

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CloneID	OCe284800
Clone ID Related Accession (Same CDS sequence)	XM_022414976.1 , XM_022414977.1
Accession Version	XM_022414977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006619.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AAGCAGGATC CTATTCTGTA ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCAGGCAGC CCTTGGAGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGAAC CGGCAGAGAT TTGTCCGAGT 
TGCCCTATTC TAGAAAATGC CCTGATAGGG CAATTTGTCG TTACTGTGGC ATCAGGGAGT 
TTCCACAATG GAGCAGTAAC AGAAAGTGGT GTAGCATACA TGTGGGGGGA GAACTCTGCA 
GGCCAGTGTG CAGTAGCTAA CCAGCAGTAT GTTCCGGAAC CAAATCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTTCTTT GTTAGCAGTC AGGATTTTGC AGTTGGCATG TGGTGAGGAG 
CACACACTGG CATTATCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG 
GGTCTCATTA CCACTACCTT CCCGGTGACA AAGCCACAAA AGGTGGAACA TCTTGCTGGG 
CGAGTAGTGC TACAAGTTGC CTGTGGTGCG TTCCACAGCT TAGCACTTGT ACAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA AGATGCAACC AGTGCAGCCA ACTCTTGATT 
ACCATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCACTGTTG CCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAAAC CAGGCCAGCA CTGCCATCAG CCCCTCCACT 
GAAACCCTTG ACAATCAAGA AGTATTCGAG AACACGCTTG TAGAAAATGA GCTGCCTGTT 
GCTACTGATA CAAACGTTGG AAATGTTCAG ACCACGGGTG GTGATGCCAT ATCCTCCCAA 
CAAGAAATCA TGGGAACAAC TGAAATTCCT TCTGCCCAAA ATATACCATC ATACCCTGAT 
ACTCAGGCAG TAAATGAGTA CATGCAAAAA CTGTCAGATC ATTCAGTAAG AGACAACTCA 
GAGAACGGCA AAAAGCCAAT GCCATCTCAG GTACCTGCTA AATTTTATAA TATAAAGCCT 
CTTGTAGAAG AAGCAGTTCC TAATCTTCAC AGTCCACCAA CCACAAGCAC CTCAGCCCTA 
AACAGCCTAG TAGTCTCTTG TGCATCTGCT GTTGGTGTGA GAGTGGCTGC TACATATGAA 
GCTGGGGCCT TGTCTCTTAA GAAAGTTATG AACTTTTATA GTACGGCCCC TTGTGAACCT 
GGAACTCAGG CAGGCAGTAG TTCCATAGGC CCAGAAGGTC TGAAAGATAG CAGAGAAGAA 
CAGGTTAAAC AGGAATCAAT GCAAGGAAAG AAAAGTTCAA GTCTCGTGGA TATCCGAGAA 
GAGGAATCAG AGGGAGGCAG CCGAAGACTC TCCCTCCCTG GATTGTTGTC CCAAGTTTCC 
CCCAGGCTCT TAAGAAAAGC TGCTCGGGTA AAAACTCGGA CAGTGGTTCT GACACCCACA 
TACAGCGGAG AAGCAGATGC ACTCCTGCCT TCTCTTAGAA CAGAAGTGTG GACCTGGGGG 
AAAGGGAAGG AAGGACAGCT GGGTCATGGT GATGTTCTGC CAAGGCTTCA ACCACTGTGT 
GTAAAATGTC TGGATGGTAA AGAAGTAATC TATCTGGAGG CAGGTGGTTA CCATTCTCTT 
GCACTTACTG CGAAATCCCA GGTTTACTCA TGGGGTAGCA ATACCTTTGG TCAACTTGGA 
CATTATGATT TTCCAACAAC AGTTCCTCGT CTTGCAAAGG TGAGCAGTGA AAATGGAGTC 
TGGAGTGTCG CTGCCGGCCA GGATTATTCC CTGTTCTTAG TGGATACAGA AGACTTCCAG 
CCTGGGTTGT ATTACAGTGG CCGACAGGAC CCTGCGGAAG GTGACAACCT TCCAGAGAAT 
CACAGTGGCA CTAAGACTCC AGTACTTCTC TCCTGTAGTA AGCTTGGATA TATAAGTAGA 
GTGACAGCAG GAAAAGATAG CTATCTAGCT TTGGTGGACA AGAATGTTAT GGGATATATT 
GCCAGTCTCC ATGAGTTGGC TACCACGGAA AGACGGTTCT ATGCAAAACT AAGTGACATC 
AAATCTCAGA TTCTCAGGCC TCTTCTCAGT TTAGAAAATT TGGGTACTGC AAGCACAGTC 
CAGCTGTTGC AGGAGGTGGC AAGCCGATTC AGCAAGCTGT GTTACCTAAT TGGTCAGCAT 
GGAGCCTCAC TGAGCAGCTT CCTTCATGGG ATAAAGGAAG CCAGGAGTTT GGTCATTCTG 
AAGCATGCAA GTCTCTTCTT GGATAGTTAT ACAGAGTATT GCACATCTAT TACAAATTTC 
CTGGTTATGG GAGGATTCCA ACTTCTTGCT AAGCCTGCCA TTGATTTCCT AAATAAAAAC 
CAGGAGCTAT TACAAGATTT ATCAGAAGTA AATGATGAAA ACACCCAATT GATGGAAATA 
CTGAATACTC TGTTTTTCTT GCCAATCAGA CGACTTCATA ATTATGCAAA AGTTTTGCTA 
AAGCTTGCTA CTTGTTTTGA AGTGACATCT CCAGAATACC AGAAACTGCA GGATTCCAGT 
TCTTGTTATG AGTGTCTTGC TCTCCATCTC GGCAGGAAAA GGAAGGAAGC TGAATACACC 
CTGGGCTTCT GGAAGACCTT TCCTGGGAAA ATGACGGATT CCTTAAGGAA GCCAGAGCGT 
CGACTGCTCT GTGAAAGCAG TAACCGGGCC CTATCTCTGC AGCATGCTGG GAGGTTTTCT 
GTGAATTGGT TCATTCTCTT TAATGATGCC CTGGTCCATG CCCAGTTCTC CACACATCAT 
GTTTTCCCTT TGGCCACACT ATGGGCAGAG CCACTTTCTG AAGAAGCTGG TGGTGTGAAT 
GGCTTAAAGA TAACTACACC TGAGGAGCAG TTCACTCTCA TTTCATCAAC ACCCCAGGAA 
AAGACTAAGT GGCTTCGGGC TATAAGCCAA GCTGTGGATC AGGCTTTGAG GGGGACAGCT 
GATCTTCCAC CTTATGGAAG TGGCAGCAGT ATTCAGAGGC AGGAACCACC CATTTCACGC 
AGTGCCAAGT ATACTTTCTA TAAGGATCCT CGCCTAAAAG ATGCAACCTA TGATGGACGC 
TGGCTTTCAG GGAAGCCTCA TGGCAGAGGG GTTTTGAAAT GGCCTGATGG AAAGATGTAT 
TCTGGCATGT TCAGGAATGG CTTGGAAGAT GGATATGGAG AATACAGAAT CCCAAACAAA 
GCATTGAACA AAGAAGACCA TTATGTAGGC CATTGGAAAG AAGGAAAAAT GTGCGGTCAA 
GGAGTCTATA GTTATGCCTC TGGTGAAGTG TTTGAAGGCT GTTTTCAAGA TAACATGCGT 
CATGGTCATG GTCTCCTTCG AAGCGGAAAA TTAACTTCCT CTTCTCCCAG TATGTTCATT 
GGCCAGTGGG TAATGGATAA GAAAGCAGGA TATGGTGTCT TTGATGATAT CACTAGAGGG 
GAAAAGTATA TGGGAATGTG GCAAGATGAC GTATGTCAAG GGAATGGTGT GGTAGTTACC 
CAATTTGGAT TATACTATGA AGGCAACTTT CACCTTAATA AAATGATGGG AAATGGTGTT 
TTACTTTCTG AAGATGATAC TATATATGAG GGAGAATTTT CAGATGACTG GACTCTTAGT 
GGAAAGGGAA CACTGACTAT GCCAAATGGA GATTACATTG AAGGTTATTT TAGTGGAGAA 
TGGGGATCTG GTATAAAAAT CACAGGAACC TACTTCAAGC CTAGTCTGTA TGAGAGTGAT 
AAAGACAGAC CCAAAGTTTT CAGGAAGCTG GGAAACCTGG CAGTGTCTGC TGATGAAAAG 
TGGAAAGCAG TATTTGATGA ATGTTGGCAT CAACTGGGCT GTGAGAACCC AGGCCAAGGG 
GAAGTTTGGA AAGCATGGGA CAATATTGCT GTGGCCCTGA CCACCAATCG GCGCCAACAC 
AGAGACAGTC CAGAGACATT AAGTCGTTCA CAGACTCAGA CACTAGAGAG TTTGGAATTC 
ATTCCTCAGC ATGTTGGTGC TTTCTCGGTA GAGAAATATG ATGACATCAA GAAATATTTA 
ATAAAGGCAT GTGACACTCC TCTGCACCCC CTGGGCAGGC TTGTGGAGAC ACTGGTTGCA 
GTTTATAGAA TGACATATGT GGGTGTAGGA GCCAATCGCC GGCTACTGCA GGAGGCTGTG 
AAAGAGATTA AATCTTACCT TAAGCGAATT TTCCAGCTGG TGAGATTCTT ATTTCCTGAG 
CTTCCTGAAG AGGGCAGCAC AATTCCTCTT TCTGCTCCTC TGCCAACGGA AAGGAAATCC 
TTTTGCACTG GGAAGTCAGA TTCCAGATCC GAATCACCAG AGCCAGGTTA TGTAGTAACA 
AGTTCTGGGT TACTGCTTCC TGTACTACTA CCTCGGCTCT ACCCACCACT GTTTATGCTC 
TATGCCCTGG ATAATGATCG TGAGGAAGAC ATTTACTGGG AGTGTGTTCT ACGACTAAAT 
AAGCAGCCAG ATATTGCTCT TCTCGGCTTC CTTGGGGTGC AGAGGAAATT TTGGCCAGTG 
ACCTTGTCAA TCCTTGGAGA GAGTAAAAAG GTTCTGCCAA CCACAAAAGA TGCTTGTTTT 
GCCTCAGCTG TAGAATGTCT ACAGCAGATC AGCACAACAT TTACCCCATC AGACAAACTT 
AAGGTCATCC AGCAGACTTT TGAAGAGATC TCTCAGAGTG TCCTGGCATC ACTTCAGGAA 
GACTTCCTGT GGTCCATGGA TGACTTGTTT CCTGTTTTCT TATATGTGGT GCTACGGGCC 
AGGATTAGGA ATTTAGGCTC TGAGGTACAC CTCATTGAAG ATCTAATGGA TCCATATTTA 
CAGCATGGGG AACAGGGTAT AATGTTCACC ACCTTAAAGG CATGTTACTA CCAGATCCAG 
CGTGAGAAGC TTAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022270685.1
CDS	161..5158
Translation	MDSKKRSSTEAEGSKERGLVHVWQAGSYSVTPERLPGWGGKTVLQAALGVKHGVLLTEDGEVYSFGTLPWRSEPAEICPSCPILENALIGQFVVTVASGSFHNGAVTESGVAYMWGENSAGQCAVANQQYVPEPNPVSISDSETSSLLAVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAENQASTAISPSTETLDNQEVFENTLVENELPVATDTNVGNVQTTGGDAISSQQEIMGTTEIPSAQNIPSYPDTQAVNEYMQKLSDHSVRDNSENGKKPMPSQVPAKFYNIKPLVEEAVPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSTAPCEPGTQAGSSSIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIYLEAGGYHSLALTAKSQVYSWGSNTFGQLGHYDFPTTVPRLAKVSSENGVWSVAAGQDYSLFLVDTEDFQPGLYYSGRQDPAEGDNLPENHSGTKTPVLLSCSKLGYISRVTAGKDSYLALVDKNVMGYIASLHELATTERRFYAKLSDIKSQILRPLLSLENLGTASTVQLLQEVASRFSKLCYLIGQHGASLSSFLHGIKEARSLVILKHASLFLDSYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVNDENTQLMEILNTLFFLPIRRLHNYAKVLLKLATCFEVTSPEYQKLQDSSSCYECLALHLGRKRKEAEYTLGFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGTADLPPYGSGSSIQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGMWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKVFRKLGNLAVSADEKWKAVFDECWHQLGCENPGQGEVWKAWDNIAVALTTNRRQHRDSPETLSRSQTQTLESLEFIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALDNDREEDIYWECVLRLNKQPDIALLGFLGVQRKFWPVTLSILGESKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_022414977.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022414977.1

ORF Insert Sequence:

ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AAGCAGGATC CTATTCTGTA ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCAGGCAGC CCTTGGAGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGAAC CGGCAGAGAT TTGTCCGAGT 
TGCCCTATTC TAGAAAATGC CCTGATAGGG CAATTTGTCG TTACTGTGGC ATCAGGGAGT 
TTCCACAATG GAGCAGTAAC AGAAAGTGGT GTAGCATACA TGTGGGGGGA GAACTCTGCA 
GGCCAGTGTG CAGTAGCTAA CCAGCAGTAT GTTCCGGAAC CAAATCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTTCTTT GTTAGCAGTC AGGATTTTGC AGTTGGCATG TGGTGAGGAG 
CACACACTGG CATTATCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG 
GGTCTCATTA CCACTACCTT CCCGGTGACA AAGCCACAAA AGGTGGAACA TCTTGCTGGG 
CGAGTAGTGC TACAAGTTGC CTGTGGTGCG TTCCACAGCT TAGCACTTGT ACAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA AGATGCAACC AGTGCAGCCA ACTCTTGATT 
ACCATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCACTGTTG CCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAAAC CAGGCCAGCA CTGCCATCAG CCCCTCCACT 
GAAACCCTTG ACAATCAAGA AGTATTCGAG AACACGCTTG TAGAAAATGA GCTGCCTGTT 
GCTACTGATA CAAACGTTGG AAATGTTCAG ACCACGGGTG GTGATGCCAT ATCCTCCCAA 
CAAGAAATCA TGGGAACAAC TGAAATTCCT TCTGCCCAAA ATATACCATC ATACCCTGAT 
ACTCAGGCAG TAAATGAGTA CATGCAAAAA CTGTCAGATC ATTCAGTAAG AGACAACTCA 
GAGAACGGCA AAAAGCCAAT GCCATCTCAG GTACCTGCTA AATTTTATAA TATAAAGCCT 
CTTGTAGAAG AAGCAGTTCC TAATCTTCAC AGTCCACCAA CCACAAGCAC CTCAGCCCTA 
AACAGCCTAG TAGTCTCTTG TGCATCTGCT GTTGGTGTGA GAGTGGCTGC TACATATGAA 
GCTGGGGCCT TGTCTCTTAA GAAAGTTATG AACTTTTATA GTACGGCCCC TTGTGAACCT 
GGAACTCAGG CAGGCAGTAG TTCCATAGGC CCAGAAGGTC TGAAAGATAG CAGAGAAGAA 
CAGGTTAAAC AGGAATCAAT GCAAGGAAAG AAAAGTTCAA GTCTCGTGGA TATCCGAGAA 
GAGGAATCAG AGGGAGGCAG CCGAAGACTC TCCCTCCCTG GATTGTTGTC CCAAGTTTCC 
CCCAGGCTCT TAAGAAAAGC TGCTCGGGTA AAAACTCGGA CAGTGGTTCT GACACCCACA 
TACAGCGGAG AAGCAGATGC ACTCCTGCCT TCTCTTAGAA CAGAAGTGTG GACCTGGGGG 
AAAGGGAAGG AAGGACAGCT GGGTCATGGT GATGTTCTGC CAAGGCTTCA ACCACTGTGT 
GTAAAATGTC TGGATGGTAA AGAAGTAATC TATCTGGAGG CAGGTGGTTA CCATTCTCTT 
GCACTTACTG CGAAATCCCA GGTTTACTCA TGGGGTAGCA ATACCTTTGG TCAACTTGGA 
CATTATGATT TTCCAACAAC AGTTCCTCGT CTTGCAAAGG TGAGCAGTGA AAATGGAGTC 
TGGAGTGTCG CTGCCGGCCA GGATTATTCC CTGTTCTTAG TGGATACAGA AGACTTCCAG 
CCTGGGTTGT ATTACAGTGG CCGACAGGAC CCTGCGGAAG GTGACAACCT TCCAGAGAAT 
CACAGTGGCA CTAAGACTCC AGTACTTCTC TCCTGTAGTA AGCTTGGATA TATAAGTAGA 
GTGACAGCAG GAAAAGATAG CTATCTAGCT TTGGTGGACA AGAATGTTAT GGGATATATT 
GCCAGTCTCC ATGAGTTGGC TACCACGGAA AGACGGTTCT ATGCAAAACT AAGTGACATC 
AAATCTCAGA TTCTCAGGCC TCTTCTCAGT TTAGAAAATT TGGGTACTGC AAGCACAGTC 
CAGCTGTTGC AGGAGGTGGC AAGCCGATTC AGCAAGCTGT GTTACCTAAT TGGTCAGCAT 
GGAGCCTCAC TGAGCAGCTT CCTTCATGGG ATAAAGGAAG CCAGGAGTTT GGTCATTCTG 
AAGCATGCAA GTCTCTTCTT GGATAGTTAT ACAGAGTATT GCACATCTAT TACAAATTTC 
CTGGTTATGG GAGGATTCCA ACTTCTTGCT AAGCCTGCCA TTGATTTCCT AAATAAAAAC 
CAGGAGCTAT TACAAGATTT ATCAGAAGTA AATGATGAAA ACACCCAATT GATGGAAATA 
CTGAATACTC TGTTTTTCTT GCCAATCAGA CGACTTCATA ATTATGCAAA AGTTTTGCTA 
AAGCTTGCTA CTTGTTTTGA AGTGACATCT CCAGAATACC AGAAACTGCA GGATTCCAGT 
TCTTGTTATG AGTGTCTTGC TCTCCATCTC GGCAGGAAAA GGAAGGAAGC TGAATACACC 
CTGGGCTTCT GGAAGACCTT TCCTGGGAAA ATGACGGATT CCTTAAGGAA GCCAGAGCGT 
CGACTGCTCT GTGAAAGCAG TAACCGGGCC CTATCTCTGC AGCATGCTGG GAGGTTTTCT 
GTGAATTGGT TCATTCTCTT TAATGATGCC CTGGTCCATG CCCAGTTCTC CACACATCAT 
GTTTTCCCTT TGGCCACACT ATGGGCAGAG CCACTTTCTG AAGAAGCTGG TGGTGTGAAT 
GGCTTAAAGA TAACTACACC TGAGGAGCAG TTCACTCTCA TTTCATCAAC ACCCCAGGAA 
AAGACTAAGT GGCTTCGGGC TATAAGCCAA GCTGTGGATC AGGCTTTGAG GGGGACAGCT 
GATCTTCCAC CTTATGGAAG TGGCAGCAGT ATTCAGAGGC AGGAACCACC CATTTCACGC 
AGTGCCAAGT ATACTTTCTA TAAGGATCCT CGCCTAAAAG ATGCAACCTA TGATGGACGC 
TGGCTTTCAG GGAAGCCTCA TGGCAGAGGG GTTTTGAAAT GGCCTGATGG AAAGATGTAT 
TCTGGCATGT TCAGGAATGG CTTGGAAGAT GGATATGGAG AATACAGAAT CCCAAACAAA 
GCATTGAACA AAGAAGACCA TTATGTAGGC CATTGGAAAG AAGGAAAAAT GTGCGGTCAA 
GGAGTCTATA GTTATGCCTC TGGTGAAGTG TTTGAAGGCT GTTTTCAAGA TAACATGCGT 
CATGGTCATG GTCTCCTTCG AAGCGGAAAA TTAACTTCCT CTTCTCCCAG TATGTTCATT 
GGCCAGTGGG TAATGGATAA GAAAGCAGGA TATGGTGTCT TTGATGATAT CACTAGAGGG 
GAAAAGTATA TGGGAATGTG GCAAGATGAC GTATGTCAAG GGAATGGTGT GGTAGTTACC 
CAATTTGGAT TATACTATGA AGGCAACTTT CACCTTAATA AAATGATGGG AAATGGTGTT 
TTACTTTCTG AAGATGATAC TATATATGAG GGAGAATTTT CAGATGACTG GACTCTTAGT 
GGAAAGGGAA CACTGACTAT GCCAAATGGA GATTACATTG AAGGTTATTT TAGTGGAGAA 
TGGGGATCTG GTATAAAAAT CACAGGAACC TACTTCAAGC CTAGTCTGTA TGAGAGTGAT 
AAAGACAGAC CCAAAGTTTT CAGGAAGCTG GGAAACCTGG CAGTGTCTGC TGATGAAAAG 
TGGAAAGCAG TATTTGATGA ATGTTGGCAT CAACTGGGCT GTGAGAACCC AGGCCAAGGG 
GAAGTTTGGA AAGCATGGGA CAATATTGCT GTGGCCCTGA CCACCAATCG GCGCCAACAC 
AGAGACAGTC CAGAGACATT AAGTCGTTCA CAGACTCAGA CACTAGAGAG TTTGGAATTC 
ATTCCTCAGC ATGTTGGTGC TTTCTCGGTA GAGAAATATG ATGACATCAA GAAATATTTA 
ATAAAGGCAT GTGACACTCC TCTGCACCCC CTGGGCAGGC TTGTGGAGAC ACTGGTTGCA 
GTTTATAGAA TGACATATGT GGGTGTAGGA GCCAATCGCC GGCTACTGCA GGAGGCTGTG 
AAAGAGATTA AATCTTACCT TAAGCGAATT TTCCAGCTGG TGAGATTCTT ATTTCCTGAG 
CTTCCTGAAG AGGGCAGCAC AATTCCTCTT TCTGCTCCTC TGCCAACGGA AAGGAAATCC 
TTTTGCACTG GGAAGTCAGA TTCCAGATCC GAATCACCAG AGCCAGGTTA TGTAGTAACA 
AGTTCTGGGT TACTGCTTCC TGTACTACTA CCTCGGCTCT ACCCACCACT GTTTATGCTC 
TATGCCCTGG ATAATGATCG TGAGGAAGAC ATTTACTGGG AGTGTGTTCT ACGACTAAAT 
AAGCAGCCAG ATATTGCTCT TCTCGGCTTC CTTGGGGTGC AGAGGAAATT TTGGCCAGTG 
ACCTTGTCAA TCCTTGGAGA GAGTAAAAAG GTTCTGCCAA CCACAAAAGA TGCTTGTTTT 
GCCTCAGCTG TAGAATGTCT ACAGCAGATC AGCACAACAT TTACCCCATC AGACAAACTT 
AAGGTCATCC AGCAGACTTT TGAAGAGATC TCTCAGAGTG TCCTGGCATC ACTTCAGGAA 
GACTTCCTGT GGTCCATGGA TGACTTGTTT CCTGTTTTCT TATATGTGGT GCTACGGGCC 
AGGATTAGGA ATTTAGGCTC TGAGGTACAC CTCATTGAAG ATCTAATGGA TCCATATTTA 
CAGCATGGGG AACAGGGTAT AATGTTCACC ACCTTAAAGG CATGTTACTA CCAGATCCAG 
CGTGAGAAGC TTAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_022414978.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022270686.1 Canis lupus familiaris ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe284801
Clone ID Related Accession (Same CDS sequence)	XM_022414978.1
Accession Version	XM_022414978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	alsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006619.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AAGCAGGATC CTATTCTGTA ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCAGGCAGC CCTTGGAGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGAAC CGGCAGAGAT TTGTCCGAGT 
TGCCCTATTC TAGAAAATGC CCTGATAGGG CAATTTGTCG TTACTGTGGC ATCAGGGAGT 
TTCCACAATG GAGCAGTAAC AGAAAGTGGT GTAGCATACA TGTGGGGGGA GAACTCTGCA 
GGCCAGTGTG CAGTAGCTAA CCAGCAGTAT GTTCCGGAAC CAAATCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTTCTTT GTTAGCAGTC AGGATTTTGC AGTTGGCATG TGGTGAGGAG 
CACACACTGG CATTATCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG 
GGTCTCATTA CCACTACCTT CCCGGTGACA AAGCCACAAA AGGTGGAACA TCTTGCTGGG 
CGAGTAGTGC TACAAGTTGC CTGTGGTGCG TTCCACAGCT TAGCACTTGT ACAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA AGATGCAACC AGTGCAGCCA ACTCTTGATT 
ACCATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCACTGTTG CCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAAAC CAGGCCAGCA CTGCCATCAG CCCCTCCACT 
GAAACCCTTG ACAATCAAGA AGTATTCGAG AACACGCTTG TAGAAAATGA GCTGCCTGTT 
GCTACTGATA CAAACGTTGG AAATGTTCAG ACCACGGGTG GTGATGCCAT ATCCTCCCAA 
CAAGAAATCA TGGGAACAAC TGAAATTCCT TCTGCCCAAA ATATACCATC ATACCCTGAT 
ACTCAGGCAG TAAATGAGTA CATGCAAAAA CTGTCAGATC ATTCAGTAAG AGACAACTCA 
GAGAACGGCA AAAAGCCAAT GCCATCTCAG GTACCTGCTA AATTTTATAA TATAAAGCCT 
CTTGTAGAAG AAGCAGTTCC TAATCTTCAC AGTCCACCAA CCACAAGCAC CTCAGCCCTA 
AACAGCCTAG TAGTCTCTTG TGCATCTGCT GTTGGTGTGA GAGTGGCTGC TACATATGAA 
GCTGGGGCCT TGTCTCTTAA GAAAGTTATG AACTTTTATA GTACGGCCCC TTGTGAACCT 
GGAACTCAGG CAGGCAGTAG TTCCATAGGC CCAGAAGGTC TGAAAGATAG CAGAGAAGAA 
CAGGTTAAAC AGGAATCAAT GCAAGGAAAG AAAAGTTCAA GTCTCGTGGA TATCCGAGAA 
GAGGAATCAG AGGGAGGCAG CCGAAGACTC TCCCTCCCTG GATTGTTGTC CCAAGTTTCC 
CCCAGGCTCT TAAGAAAAGC TGCTCGGGTA AAAACTCGGA CAGTGGTTCT GACACCCACA 
TACAGCGGAG AAGCAGATGC ACTCCTGCCT TCTCTTAGAA CAGAAGTGTG GACCTGGGGG 
AAAGGGAAGG AAGGACAGCT GGGTCATGGT GATGTTCTGC CAAGGCTTCA ACCACTGTGT 
GTAAAATGTC TGGATGGTAA AGAAGTAATC TATCTGGAGG CAGGTGGTTA CCATTCTCTT 
GCACTTACTG CGAAATCCCA GGTTTACTCA TGGGGTAGCA ATACCTTTGG TCAACTTGGA 
CATTATGATT TTCCAACAAC AGTTCCTCGT CTTGCAAAGG TGAGCAGTGA AAATGGAGTC 
TGGAGTGTCG CTGCCGGCCA GGATTATTCC CTGTTCTTAG TGGATACAGA AGACTTCCAG 
CCTGGGTTGT ATTACAGTGG CCGACAGGAC CCTGCGGAAG GTGACAACCT TCCAGAGAAT 
CACAGTGGCA CTAAGACTCC AGTACTTCTC TCCTGTAGTA AGCTTGGATA TATAAGTAGA 
GTGACAGCAG GAAAAGATAG CTATCTAGCT TTGGTGGACA AGAATGTTAT GGGATATATT 
GCCAGTCTCC ATGAGTTGGC TACCACGGAA AGACGGTTCT ATGCAAAACT AAGTGACATC 
AAATCTCAGA TTCTCAGGCC TCTTCTCAGT TTAGAAAATT TGGGTACTGC AAGCACAGTC 
CAGCTGTTGC AGGAGGTGGC AAGCCGATTC AGCAAGCTGT GTTACCTAAT TGGTCAGCAT 
GGAGCCTCAC TGAGCAGCTT CCTTCATGGG ATAAAGGAAG CCAGGAGTTT GGTCATTCTG 
AAGCATGCAA GTCTCTTCTT GGATAGTTAT ACAGAGTATT GCACATCTAT TACAAATTTC 
CTGGTTATGG GAGGATTCCA ACTTCTTGCT AAGCCTGCCA TTGATTTCCT AAATAAAAAC 
CAGGAGCTAT TACAAGATTT ATCAGAAGTA AATGATGAAA ACACCCAATT GATGGAAATA 
CTGAATACTC TGTTTTTCTT GCCAATCAGA CGACTTCATA ATTATGCAAA AGTTTTGCTA 
AAGCTTGCTA CTTGTTTTGA AGTGACATCT CCAGAATACC AGAAACTGCA GGATTCCAGT 
TCTTGTTATG AGTGTCTTGC TCTCCATCTC GGCAGGAAAA GGAAGGAAGC TGAATACACC 
CTGGGCTTCT GGAAGACCTT TCCTGGGAAA ATGACGGATT CCTTAAGGAA GCCAGAGCGT 
CGACTGCTCT GTGAAAGCAG TAACCGGGCC CTATCTCTGC AGCATGCTGG GAGGTTTTCT 
GTGAATTGGT TCATTCTCTT TAATGATGCC CTGGTCCATG CCCAGTTCTC CACACATCAT 
GTTTTCCCTT TGGCCACACT ATGGGCAGAG CCACTTTCTG AAGAAGCTGG TGGTGTGAAT 
GGCTTAAAGA TAACTACACC TGAGGAGCAG TTCACTCTCA TTTCATCAAC ACCCCAGGAA 
AAGACTAAGT GGCTTCGGGC TATAAGCCAA GCTGTGGATC AGGCTTTGAG GGGGACAGCT 
GATCTTCCAC CTTATGGAAG TGGCAGCAGT ATTCAGAGGC AGGAACCACC CATTTCACGC 
AGTGCCAAGT ATACTTTCTA TAAGGATCCT CGCCTAAAAG ATGCAACCTA TGATGGACGC 
TGGCTTTCAG GGAAGCCTCA TGGCAGAGGG GTTTTGAAAT GGCCTGATGG AAAGATGTAT 
TCTGGCATGT TCAGGAATGG CTTGGAAGAT GGATATGGAG AATACAGAAT CCCAAACAAA 
GCATTGAACA AAGAAGACCA TTATGTAGGC CATTGGAAAG AAGGAAAAAT GTGCGGTCAA 
GGAGTCTATA GTTATGCCTC TGGTGAAGTG TTTGAAGGCT GTTTTCAAGA TAACATGCGT 
CATGGTCATG GTCTCCTTCG AAGCGGAAAA TTAACTTCCT CTTCTCCCAG TATGTTCATT 
GGCCAGTGGG TAATGGATAA GAAAGCAGGA TATGGTGTCT TTGATGATAT CACTAGAGGG 
GAAAAGTATA TGGGAATGTG GCAAGATGAC GTATGTCAAG GGAATGGTGT GGTAGTTACC 
CAATTTGGAT TATACTATGA AGGCAACTTT CACCTTAATA AAATGATGGG AAATGGTGTT 
TTACTTTCTG AAGATGATAC TATATATGAG GGAGAATTTT CAGATGACTG GACTCTTAGT 
GGAAAGGGAA CACTGACTAT GCCAAATGGA GATTACATTG AAGGTTATTT TAGTGGAGAA 
TGGGGATCTG GTATAAAAAT CACAGGAACC TACTTCAAGC CTAGTCTGTA TGAGAGTGAT 
AAAGACAGAC CCAAAGTTTT GAAGCTGGGA AACCTGGCAG TGTCTGCTGA TGAAAAGTGG 
AAAGCAGTAT TTGATGAATG TTGGCATCAA CTGGGCTGTG AGAACCCAGG CCAAGGGGAA 
GTTTGGAAAG CATGGGACAA TATTGCTGTG GCCCTGACCA CCAATCGGCG CCAACACAGA 
GACAGTCCAG AGACATTAAG TCGTTCACAG ACTCAGACAC TAGAGAGTTT GGAATTCATT 
CCTCAGCATG TTGGTGCTTT CTCGGTAGAG AAATATGATG ACATCAAGAA ATATTTAATA 
AAGGCATGTG ACACTCCTCT GCACCCCCTG GGCAGGCTTG TGGAGACACT GGTTGCAGTT 
TATAGAATGA CATATGTGGG TGTAGGAGCC AATCGCCGGC TACTGCAGGA GGCTGTGAAA 
GAGATTAAAT CTTACCTTAA GCGAATTTTC CAGCTGGTGA GATTCTTATT TCCTGAGCTT 
CCTGAAGAGG GCAGCACAAT TCCTCTTTCT GCTCCTCTGC CAACGGAAAG GAAATCCTTT 
TGCACTGGGA AGTCAGATTC CAGATCCGAA TCACCAGAGC CAGGTTATGT AGTAACAAGT 
TCTGGGTTAC TGCTTCCTGT ACTACTACCT CGGCTCTACC CACCACTGTT TATGCTCTAT 
GCCCTGGATA ATGATCGTGA GGAAGACATT TACTGGGAGT GTGTTCTACG ACTAAATAAG 
CAGCCAGATA TTGCTCTTCT CGGCTTCCTT GGGGTGCAGA GGAAATTTTG GCCAGTGACC 
TTGTCAATCC TTGGAGAGAG TAAAAAGGTT CTGCCAACCA CAAAAGATGC TTGTTTTGCC 
TCAGCTGTAG AATGTCTACA GCAGATCAGC ACAACATTTA CCCCATCAGA CAAACTTAAG 
GTCATCCAGC AGACTTTTGA AGAGATCTCT CAGAGTGTCC TGGCATCACT TCAGGAAGAC 
TTCCTGTGGT CCATGGATGA CTTGTTTCCT GTTTTCTTAT ATGTGGTGCT ACGGGCCAGG 
ATTAGGAATT TAGGCTCTGA GGTACACCTC ATTGAAGATC TAATGGATCC ATATTTACAG 
CATGGGGAAC AGGGTATAAT GTTCACCACC TTAAAGGCAT GTTACTACCA GATCCAGCGT 
GAGAAGCTTA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022270686.1
CDS	213..5207
Translation	MDSKKRSSTEAEGSKERGLVHVWQAGSYSVTPERLPGWGGKTVLQAALGVKHGVLLTEDGEVYSFGTLPWRSEPAEICPSCPILENALIGQFVVTVASGSFHNGAVTESGVAYMWGENSAGQCAVANQQYVPEPNPVSISDSETSSLLAVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAENQASTAISPSTETLDNQEVFENTLVENELPVATDTNVGNVQTTGGDAISSQQEIMGTTEIPSAQNIPSYPDTQAVNEYMQKLSDHSVRDNSENGKKPMPSQVPAKFYNIKPLVEEAVPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSTAPCEPGTQAGSSSIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIYLEAGGYHSLALTAKSQVYSWGSNTFGQLGHYDFPTTVPRLAKVSSENGVWSVAAGQDYSLFLVDTEDFQPGLYYSGRQDPAEGDNLPENHSGTKTPVLLSCSKLGYISRVTAGKDSYLALVDKNVMGYIASLHELATTERRFYAKLSDIKSQILRPLLSLENLGTASTVQLLQEVASRFSKLCYLIGQHGASLSSFLHGIKEARSLVILKHASLFLDSYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVNDENTQLMEILNTLFFLPIRRLHNYAKVLLKLATCFEVTSPEYQKLQDSSSCYECLALHLGRKRKEAEYTLGFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGTADLPPYGSGSSIQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGMWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKVLKLGNLAVSADEKWKAVFDECWHQLGCENPGQGEVWKAWDNIAVALTTNRRQHRDSPETLSRSQTQTLESLEFIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALDNDREEDIYWECVLRLNKQPDIALLGFLGVQRKFWPVTLSILGESKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_022414978.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022414978.1

ORF Insert Sequence:

ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AAGCAGGATC CTATTCTGTA ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCAGGCAGC CCTTGGAGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGAAC CGGCAGAGAT TTGTCCGAGT 
TGCCCTATTC TAGAAAATGC CCTGATAGGG CAATTTGTCG TTACTGTGGC ATCAGGGAGT 
TTCCACAATG GAGCAGTAAC AGAAAGTGGT GTAGCATACA TGTGGGGGGA GAACTCTGCA 
GGCCAGTGTG CAGTAGCTAA CCAGCAGTAT GTTCCGGAAC CAAATCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTTCTTT GTTAGCAGTC AGGATTTTGC AGTTGGCATG TGGTGAGGAG 
CACACACTGG CATTATCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG 
GGTCTCATTA CCACTACCTT CCCGGTGACA AAGCCACAAA AGGTGGAACA TCTTGCTGGG 
CGAGTAGTGC TACAAGTTGC CTGTGGTGCG TTCCACAGCT TAGCACTTGT ACAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA AGATGCAACC AGTGCAGCCA ACTCTTGATT 
ACCATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCACTGTTG CCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAAAC CAGGCCAGCA CTGCCATCAG CCCCTCCACT 
GAAACCCTTG ACAATCAAGA AGTATTCGAG AACACGCTTG TAGAAAATGA GCTGCCTGTT 
GCTACTGATA CAAACGTTGG AAATGTTCAG ACCACGGGTG GTGATGCCAT ATCCTCCCAA 
CAAGAAATCA TGGGAACAAC TGAAATTCCT TCTGCCCAAA ATATACCATC ATACCCTGAT 
ACTCAGGCAG TAAATGAGTA CATGCAAAAA CTGTCAGATC ATTCAGTAAG AGACAACTCA 
GAGAACGGCA AAAAGCCAAT GCCATCTCAG GTACCTGCTA AATTTTATAA TATAAAGCCT 
CTTGTAGAAG AAGCAGTTCC TAATCTTCAC AGTCCACCAA CCACAAGCAC CTCAGCCCTA 
AACAGCCTAG TAGTCTCTTG TGCATCTGCT GTTGGTGTGA GAGTGGCTGC TACATATGAA 
GCTGGGGCCT TGTCTCTTAA GAAAGTTATG AACTTTTATA GTACGGCCCC TTGTGAACCT 
GGAACTCAGG CAGGCAGTAG TTCCATAGGC CCAGAAGGTC TGAAAGATAG CAGAGAAGAA 
CAGGTTAAAC AGGAATCAAT GCAAGGAAAG AAAAGTTCAA GTCTCGTGGA TATCCGAGAA 
GAGGAATCAG AGGGAGGCAG CCGAAGACTC TCCCTCCCTG GATTGTTGTC CCAAGTTTCC 
CCCAGGCTCT TAAGAAAAGC TGCTCGGGTA AAAACTCGGA CAGTGGTTCT GACACCCACA 
TACAGCGGAG AAGCAGATGC ACTCCTGCCT TCTCTTAGAA CAGAAGTGTG GACCTGGGGG 
AAAGGGAAGG AAGGACAGCT GGGTCATGGT GATGTTCTGC CAAGGCTTCA ACCACTGTGT 
GTAAAATGTC TGGATGGTAA AGAAGTAATC TATCTGGAGG CAGGTGGTTA CCATTCTCTT 
GCACTTACTG CGAAATCCCA GGTTTACTCA TGGGGTAGCA ATACCTTTGG TCAACTTGGA 
CATTATGATT TTCCAACAAC AGTTCCTCGT CTTGCAAAGG TGAGCAGTGA AAATGGAGTC 
TGGAGTGTCG CTGCCGGCCA GGATTATTCC CTGTTCTTAG TGGATACAGA AGACTTCCAG 
CCTGGGTTGT ATTACAGTGG CCGACAGGAC CCTGCGGAAG GTGACAACCT TCCAGAGAAT 
CACAGTGGCA CTAAGACTCC AGTACTTCTC TCCTGTAGTA AGCTTGGATA TATAAGTAGA 
GTGACAGCAG GAAAAGATAG CTATCTAGCT TTGGTGGACA AGAATGTTAT GGGATATATT 
GCCAGTCTCC ATGAGTTGGC TACCACGGAA AGACGGTTCT ATGCAAAACT AAGTGACATC 
AAATCTCAGA TTCTCAGGCC TCTTCTCAGT TTAGAAAATT TGGGTACTGC AAGCACAGTC 
CAGCTGTTGC AGGAGGTGGC AAGCCGATTC AGCAAGCTGT GTTACCTAAT TGGTCAGCAT 
GGAGCCTCAC TGAGCAGCTT CCTTCATGGG ATAAAGGAAG CCAGGAGTTT GGTCATTCTG 
AAGCATGCAA GTCTCTTCTT GGATAGTTAT ACAGAGTATT GCACATCTAT TACAAATTTC 
CTGGTTATGG GAGGATTCCA ACTTCTTGCT AAGCCTGCCA TTGATTTCCT AAATAAAAAC 
CAGGAGCTAT TACAAGATTT ATCAGAAGTA AATGATGAAA ACACCCAATT GATGGAAATA 
CTGAATACTC TGTTTTTCTT GCCAATCAGA CGACTTCATA ATTATGCAAA AGTTTTGCTA 
AAGCTTGCTA CTTGTTTTGA AGTGACATCT CCAGAATACC AGAAACTGCA GGATTCCAGT 
TCTTGTTATG AGTGTCTTGC TCTCCATCTC GGCAGGAAAA GGAAGGAAGC TGAATACACC 
CTGGGCTTCT GGAAGACCTT TCCTGGGAAA ATGACGGATT CCTTAAGGAA GCCAGAGCGT 
CGACTGCTCT GTGAAAGCAG TAACCGGGCC CTATCTCTGC AGCATGCTGG GAGGTTTTCT 
GTGAATTGGT TCATTCTCTT TAATGATGCC CTGGTCCATG CCCAGTTCTC CACACATCAT 
GTTTTCCCTT TGGCCACACT ATGGGCAGAG CCACTTTCTG AAGAAGCTGG TGGTGTGAAT 
GGCTTAAAGA TAACTACACC TGAGGAGCAG TTCACTCTCA TTTCATCAAC ACCCCAGGAA 
AAGACTAAGT GGCTTCGGGC TATAAGCCAA GCTGTGGATC AGGCTTTGAG GGGGACAGCT 
GATCTTCCAC CTTATGGAAG TGGCAGCAGT ATTCAGAGGC AGGAACCACC CATTTCACGC 
AGTGCCAAGT ATACTTTCTA TAAGGATCCT CGCCTAAAAG ATGCAACCTA TGATGGACGC 
TGGCTTTCAG GGAAGCCTCA TGGCAGAGGG GTTTTGAAAT GGCCTGATGG AAAGATGTAT 
TCTGGCATGT TCAGGAATGG CTTGGAAGAT GGATATGGAG AATACAGAAT CCCAAACAAA 
GCATTGAACA AAGAAGACCA TTATGTAGGC CATTGGAAAG AAGGAAAAAT GTGCGGTCAA 
GGAGTCTATA GTTATGCCTC TGGTGAAGTG TTTGAAGGCT GTTTTCAAGA TAACATGCGT 
CATGGTCATG GTCTCCTTCG AAGCGGAAAA TTAACTTCCT CTTCTCCCAG TATGTTCATT 
GGCCAGTGGG TAATGGATAA GAAAGCAGGA TATGGTGTCT TTGATGATAT CACTAGAGGG 
GAAAAGTATA TGGGAATGTG GCAAGATGAC GTATGTCAAG GGAATGGTGT GGTAGTTACC 
CAATTTGGAT TATACTATGA AGGCAACTTT CACCTTAATA AAATGATGGG AAATGGTGTT 
TTACTTTCTG AAGATGATAC TATATATGAG GGAGAATTTT CAGATGACTG GACTCTTAGT 
GGAAAGGGAA CACTGACTAT GCCAAATGGA GATTACATTG AAGGTTATTT TAGTGGAGAA 
TGGGGATCTG GTATAAAAAT CACAGGAACC TACTTCAAGC CTAGTCTGTA TGAGAGTGAT 
AAAGACAGAC CCAAAGTTTT GAAGCTGGGA AACCTGGCAG TGTCTGCTGA TGAAAAGTGG 
AAAGCAGTAT TTGATGAATG TTGGCATCAA CTGGGCTGTG AGAACCCAGG CCAAGGGGAA 
GTTTGGAAAG CATGGGACAA TATTGCTGTG GCCCTGACCA CCAATCGGCG CCAACACAGA 
GACAGTCCAG AGACATTAAG TCGTTCACAG ACTCAGACAC TAGAGAGTTT GGAATTCATT 
CCTCAGCATG TTGGTGCTTT CTCGGTAGAG AAATATGATG ACATCAAGAA ATATTTAATA 
AAGGCATGTG ACACTCCTCT GCACCCCCTG GGCAGGCTTG TGGAGACACT GGTTGCAGTT 
TATAGAATGA CATATGTGGG TGTAGGAGCC AATCGCCGGC TACTGCAGGA GGCTGTGAAA 
GAGATTAAAT CTTACCTTAA GCGAATTTTC CAGCTGGTGA GATTCTTATT TCCTGAGCTT 
CCTGAAGAGG GCAGCACAAT TCCTCTTTCT GCTCCTCTGC CAACGGAAAG GAAATCCTTT 
TGCACTGGGA AGTCAGATTC CAGATCCGAA TCACCAGAGC CAGGTTATGT AGTAACAAGT 
TCTGGGTTAC TGCTTCCTGT ACTACTACCT CGGCTCTACC CACCACTGTT TATGCTCTAT 
GCCCTGGATA ATGATCGTGA GGAAGACATT TACTGGGAGT GTGTTCTACG ACTAAATAAG 
CAGCCAGATA TTGCTCTTCT CGGCTTCCTT GGGGTGCAGA GGAAATTTTG GCCAGTGACC 
TTGTCAATCC TTGGAGAGAG TAAAAAGGTT CTGCCAACCA CAAAAGATGC TTGTTTTGCC 
TCAGCTGTAG AATGTCTACA GCAGATCAGC ACAACATTTA CCCCATCAGA CAAACTTAAG 
GTCATCCAGC AGACTTTTGA AGAGATCTCT CAGAGTGTCC TGGCATCACT TCAGGAAGAC 
TTCCTGTGGT CCATGGATGA CTTGTTTCCT GTTTTCTTAT ATGTGGTGCT ACGGGCCAGG 
ATTAGGAATT TAGGCTCTGA GGTACACCTC ATTGAAGATC TAATGGATCC ATATTTACAG 
CATGGGGAAC AGGGTATAAT GTTCACCACC TTAAAGGCAT GTTACTACCA GATCCAGCGT 
GAGAAGCTTA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_014111067.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966542.1 Canis lupus familiaris amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X3, mRNA.

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CloneID	OCe45243
Clone ID Related Accession (Same CDS sequence)	XM_014111067.1
Accession Version	XM_014111067.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	alsin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726123.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AAGCAGGATC CTATTCTGTA ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCAGGCAGC CCTTGGAGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGAAC CGGCAGAGAT TTGTCCGAGT 
TGCCCTATTC TAGAAAATGC CCTGATAGGG CAATTTGTCG TTACTGTGGC ATCAGGGAGT 
TTCCACAATG GAGCAGTAAC AGAAAGTGGT GTAGCATACA TGTGGGGGGA GAACTCTGCA 
GGCCAGTGTG CAGTAGCTAA CCAGCAGTAT GTTCCGGAAC CAAATCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTTCTTT GTTAGCAGTC AGGATTTTGC AGTTGGCATG TGGTGAGGAG 
CACACACTGG CATTATCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG 
GGTCTCATTA CCACTACCTT CCCGGTGACA AAGCCACAAA AGGTGGAACA TCTTGCTGGG 
CGAGTAGTGC TACAAGTTGC CTGTGGTGCG TTCCACAGCT TAGCACTTGT ACAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA AGATGCAACC AGTGCAGCCA ACTCTTGATT 
ACCATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCACTGTTG CCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAAAC CAGGCCAGCA CTGCCATCAG CCCCTCCACT 
GAAACCCTTG ACAATCAAGA AGTATTCGAG AACACGCTTG TAGAAAATGA GCTGCCTGTT 
GCTACTGATA CAAACGTTGG AAATGTTCAG ACCACGGGTG GTGATGCCAT ATCCTCCCAA 
CAAGAAATCA TGGGAACAAC TGAAATTCCT TCTGCCCAAA ATATACCATC ATACCCTGAT 
ACTCAGGCAG TAAATGAGTA CATGCAAAAA CTGTCAGATC ATTCAGTAAG AGACAACTCA 
GAGAACGGCA AAAAGCCAAT GCCATCTCAG CCTCTTGTAG AAGAAGCAGT TCCTAATCTT 
CACAGTCCAC CAACCACAAG CACCTCAGCC CTAAACAGCC TAGTAGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAGTACGGC CCCTTGTGAA CCTGGAACTC AGGCAGGCAG TAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAATC AATGCAAGGA 
AAGAAAAGTT CAAGTCTCGT GGATATCCGA GAAGAGGAAT CAGAGGGAGG CAGCCGAAGA 
CTCTCCCTCC CTGGATTGTT GTCCCAAGTT TCCCCCAGGC TCTTAAGAAA AGCTGCTCGG 
GTAAAAACTC GGACAGTGGT TCTGACACCC ACATACAGCG GAGAAGCAGA TGCACTCCTG 
CCTTCTCTTA GAACAGAAGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGTCAT 
GGTGATGTTC TGCCAAGGCT TCAACCACTG TGTGTAAAAT GTCTGGATGG TAAAGAAGTA 
ATCTATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCGAAATC CCAGGTTTAC 
TCATGGGGTA GCAATACCTT TGGTCAACTT GGACATTATG ATTTTCCAAC AACAGTTCCT 
CGTCTTGCAA AGGTGAGCAG TGAAAATGGA GTCTGGAGTG TCGCTGCCGG CCAGGATTAT 
TCCCTGTTCT TAGTGGATAC AGAAGACTTC CAGCCTGGGT TGTATTACAG TGGCCGACAG 
GACCCTGCGG AAGGTGACAA CCTTCCAGAG AATCACAGTG GCACTAAGAC TCCAGTACTT 
CTCTCCTGTA GTAAGCTTGG ATATATAAGT AGAGTGACAG CAGGAAAAGA TAGCTATCTA 
GCTTTGGTGG ACAAGAATGT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACCACG 
GAAAGACGGT TCTATGCAAA ACTAAGTGAC ATCAAATCTC AGATTCTCAG GCCTCTTCTC 
AGTTTAGAAA ATTTGGGTAC TGCAAGCACA GTCCAGCTGT TGCAGGAGGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAGCCT CACTGAGCAG CTTCCTTCAT 
GGGATAAAGG AAGCCAGGAG TTTGGTCATT CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAACTTCTT 
GCTAAGCCTG CCATTGATTT CCTAAATAAA AACCAGGAGC TATTACAAGA TTTATCAGAA 
GTAAATGATG AAAACACCCA ATTGATGGAA ATACTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGACTTC ATAATTATGC AAAAGTTTTG CTAAAGCTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAACT GCAGGATTCC AGTTCTTGTT ATGAGTGTCT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCTGAATAC ACCCTGGGCT TCTGGAAGAC CTTTCCTGGG 
AAAATGACGG ATTCCTTAAG GAAGCCAGAG CGTCGACTGC TCTGTGAAAG CAGTAACCGG 
GCCCTATCTC TGCAGCATGC TGGGAGGTTT TCTGTGAATT GGTTCATTCT CTTTAATGAT 
GCCCTGGTCC ATGCCCAGTT CTCCACACAT CATGTTTTCC CTTTGGCCAC ACTATGGGCA 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCTTAA AGATAACTAC ACCTGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACTA AGTGGCTTCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGACA GCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCCATTTCA CGCAGTGCCA AGTATACTTT CTATAAGGAT 
CCTCGCCTAA AAGATGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AATGGCCTGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATATG GAGAATACAG AATCCCAAAC AAAGCATTGA ACAAAGAAGA CCATTATGTA 
GGCCATTGGA AAGAAGGAAA AATGTGCGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAACATG CGTCATGGTC ATGGTCTCCT TCGAAGCGGA 
AAATTAACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACTAGA GGGGAAAAGT ATATGGGAAT GTGGCAAGAT 
GACGTATGTC AAGGGAATGG TGTGGTAGTT ACCCAATTTG GATTATACTA TGAAGGCAAC 
TTTCACCTTA ATAAAATGAT GGGAAATGGT GTTTTACTTT CTGAAGATGA TACTATATAT 
GAGGGAGAAT TTTCAGATGA CTGGACTCTT AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGTATAAA AATCACAGGA 
ACCTACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACA GACCCAAAGT TTTGAAGCTG 
GGAAACCTGG CAGTGTCTGC TGATGAAAAG TGGAAAGCAG TATTTGATGA ATGTTGGCAT 
CAACTGGGCT GTGAGAACCC AGGCCAAGGG GAAGTTTGGA AAGCATGGGA CAATATTGCT 
GTGGCCCTGA CCACCAATCG GCGCCAACAC AGAGACAGTC CAGAGACATT AAGTCGTTCA 
CAGACTCAGA CACTAGAGAG TTTGGAATTC ATTCCTCAGC ATGTTGGTGC TTTCTCGGTA 
GAGAAATATG ATGACATCAA GAAATATTTA ATAAAGGCAT GTGACACTCC TCTGCACCCC 
CTGGGCAGGC TTGTGGAGAC ACTGGTTGCA GTTTATAGAA TGACATATGT GGGTGTAGGA 
GCCAATCGCC GGCTACTGCA GGAGGCTGTG AAAGAGATTA AATCTTACCT TAAGCGAATT 
TTCCAGCTGG TGAGATTCTT ATTTCCTGAG CTTCCTGAAG AGGGCAGCAC AATTCCTCTT 
TCTGCTCCTC TGCCAACGGA AAGGAAATCC TTTTGCACTG GGAAGTCAGA TTCCAGATCC 
GAATCACCAG AGCCAGGTTA TGTAGTAACA AGTTCTGGGT TACTGCTTCC TGTACTACTA 
CCTCGGCTCT ACCCACCACT GTTTATGCTC TATGCCCTGG ATAATGATCG TGAGGAAGAC 
ATTTACTGGG AGTGTGTTCT ACGACTAAAT AAGCAGCCAG ATATTGCTCT TCTCGGCTTC 
CTTGGGGTGC AGAGGAAATT TTGGCCAGTG ACCTTGTCAA TCCTTGGAGA GAGTAAAAAG 
GTTCTGCCAA CCACAAAAGA TGCTTGTTTT GCCTCAGCTG TAGAATGTCT ACAGCAGATC 
AGCACAACAT TTACCCCATC AGACAAACTT AAGGTCATCC AGCAGACTTT TGAAGAGATC 
TCTCAGAGTG TCCTGGCATC ACTTCAGGAA GACTTCCTGT GGTCCATGGA TGACTTGTTT 
CCTGTTTTCT TATATGTGGT GCTACGGGCC AGGATTAGGA ATTTAGGCTC TGAGGTACAC 
CTCATTGAAG ATCTAATGGA TCCATATTTA CAGCATGGGG AACAGGGTAT AATGTTCACC 
ACCTTAAAGG CATGTTACTA CCAGATCCAG CGTGAGAAGC TTAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966542.1
CDS	154..5121
Translation	MDSKKRSSTEAEGSKERGLVHVWQAGSYSVTPERLPGWGGKTVLQAALGVKHGVLLTEDGEVYSFGTLPWRSEPAEICPSCPILENALIGQFVVTVASGSFHNGAVTESGVAYMWGENSAGQCAVANQQYVPEPNPVSISDSETSSLLAVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAENQASTAISPSTETLDNQEVFENTLVENELPVATDTNVGNVQTTGGDAISSQQEIMGTTEIPSAQNIPSYPDTQAVNEYMQKLSDHSVRDNSENGKKPMPSQPLVEEAVPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSTAPCEPGTQAGSSSIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIYLEAGGYHSLALTAKSQVYSWGSNTFGQLGHYDFPTTVPRLAKVSSENGVWSVAAGQDYSLFLVDTEDFQPGLYYSGRQDPAEGDNLPENHSGTKTPVLLSCSKLGYISRVTAGKDSYLALVDKNVMGYIASLHELATTERRFYAKLSDIKSQILRPLLSLENLGTASTVQLLQEVASRFSKLCYLIGQHGASLSSFLHGIKEARSLVILKHASLFLDSYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVNDENTQLMEILNTLFFLPIRRLHNYAKVLLKLATCFEVTSPEYQKLQDSSSCYECLALHLGRKRKEAEYTLGFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGTADLPPYGSGSSIQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGMWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKVLKLGNLAVSADEKWKAVFDECWHQLGCENPGQGEVWKAWDNIAVALTTNRRQHRDSPETLSRSQTQTLESLEFIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALDNDREEDIYWECVLRLNKQPDIALLGFLGVQRKFWPVTLSILGESKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_014111067.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111067.1

ORF Insert Sequence:

ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AAGCAGGATC CTATTCTGTA ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCAGGCAGC CCTTGGAGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGAAC CGGCAGAGAT TTGTCCGAGT 
TGCCCTATTC TAGAAAATGC CCTGATAGGG CAATTTGTCG TTACTGTGGC ATCAGGGAGT 
TTCCACAATG GAGCAGTAAC AGAAAGTGGT GTAGCATACA TGTGGGGGGA GAACTCTGCA 
GGCCAGTGTG CAGTAGCTAA CCAGCAGTAT GTTCCGGAAC CAAATCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTTCTTT GTTAGCAGTC AGGATTTTGC AGTTGGCATG TGGTGAGGAG 
CACACACTGG CATTATCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG 
GGTCTCATTA CCACTACCTT CCCGGTGACA AAGCCACAAA AGGTGGAACA TCTTGCTGGG 
CGAGTAGTGC TACAAGTTGC CTGTGGTGCG TTCCACAGCT TAGCACTTGT ACAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA AGATGCAACC AGTGCAGCCA ACTCTTGATT 
ACCATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCACTGTTG CCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAAAC CAGGCCAGCA CTGCCATCAG CCCCTCCACT 
GAAACCCTTG ACAATCAAGA AGTATTCGAG AACACGCTTG TAGAAAATGA GCTGCCTGTT 
GCTACTGATA CAAACGTTGG AAATGTTCAG ACCACGGGTG GTGATGCCAT ATCCTCCCAA 
CAAGAAATCA TGGGAACAAC TGAAATTCCT TCTGCCCAAA ATATACCATC ATACCCTGAT 
ACTCAGGCAG TAAATGAGTA CATGCAAAAA CTGTCAGATC ATTCAGTAAG AGACAACTCA 
GAGAACGGCA AAAAGCCAAT GCCATCTCAG CCTCTTGTAG AAGAAGCAGT TCCTAATCTT 
CACAGTCCAC CAACCACAAG CACCTCAGCC CTAAACAGCC TAGTAGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAGTACGGC CCCTTGTGAA CCTGGAACTC AGGCAGGCAG TAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAATC AATGCAAGGA 
AAGAAAAGTT CAAGTCTCGT GGATATCCGA GAAGAGGAAT CAGAGGGAGG CAGCCGAAGA 
CTCTCCCTCC CTGGATTGTT GTCCCAAGTT TCCCCCAGGC TCTTAAGAAA AGCTGCTCGG 
GTAAAAACTC GGACAGTGGT TCTGACACCC ACATACAGCG GAGAAGCAGA TGCACTCCTG 
CCTTCTCTTA GAACAGAAGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGTCAT 
GGTGATGTTC TGCCAAGGCT TCAACCACTG TGTGTAAAAT GTCTGGATGG TAAAGAAGTA 
ATCTATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCGAAATC CCAGGTTTAC 
TCATGGGGTA GCAATACCTT TGGTCAACTT GGACATTATG ATTTTCCAAC AACAGTTCCT 
CGTCTTGCAA AGGTGAGCAG TGAAAATGGA GTCTGGAGTG TCGCTGCCGG CCAGGATTAT 
TCCCTGTTCT TAGTGGATAC AGAAGACTTC CAGCCTGGGT TGTATTACAG TGGCCGACAG 
GACCCTGCGG AAGGTGACAA CCTTCCAGAG AATCACAGTG GCACTAAGAC TCCAGTACTT 
CTCTCCTGTA GTAAGCTTGG ATATATAAGT AGAGTGACAG CAGGAAAAGA TAGCTATCTA 
GCTTTGGTGG ACAAGAATGT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACCACG 
GAAAGACGGT TCTATGCAAA ACTAAGTGAC ATCAAATCTC AGATTCTCAG GCCTCTTCTC 
AGTTTAGAAA ATTTGGGTAC TGCAAGCACA GTCCAGCTGT TGCAGGAGGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAGCCT CACTGAGCAG CTTCCTTCAT 
GGGATAAAGG AAGCCAGGAG TTTGGTCATT CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAACTTCTT 
GCTAAGCCTG CCATTGATTT CCTAAATAAA AACCAGGAGC TATTACAAGA TTTATCAGAA 
GTAAATGATG AAAACACCCA ATTGATGGAA ATACTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGACTTC ATAATTATGC AAAAGTTTTG CTAAAGCTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAACT GCAGGATTCC AGTTCTTGTT ATGAGTGTCT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCTGAATAC ACCCTGGGCT TCTGGAAGAC CTTTCCTGGG 
AAAATGACGG ATTCCTTAAG GAAGCCAGAG CGTCGACTGC TCTGTGAAAG CAGTAACCGG 
GCCCTATCTC TGCAGCATGC TGGGAGGTTT TCTGTGAATT GGTTCATTCT CTTTAATGAT 
GCCCTGGTCC ATGCCCAGTT CTCCACACAT CATGTTTTCC CTTTGGCCAC ACTATGGGCA 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCTTAA AGATAACTAC ACCTGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACTA AGTGGCTTCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGACA GCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCCATTTCA CGCAGTGCCA AGTATACTTT CTATAAGGAT 
CCTCGCCTAA AAGATGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AATGGCCTGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATATG GAGAATACAG AATCCCAAAC AAAGCATTGA ACAAAGAAGA CCATTATGTA 
GGCCATTGGA AAGAAGGAAA AATGTGCGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAACATG CGTCATGGTC ATGGTCTCCT TCGAAGCGGA 
AAATTAACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACTAGA GGGGAAAAGT ATATGGGAAT GTGGCAAGAT 
GACGTATGTC AAGGGAATGG TGTGGTAGTT ACCCAATTTG GATTATACTA TGAAGGCAAC 
TTTCACCTTA ATAAAATGAT GGGAAATGGT GTTTTACTTT CTGAAGATGA TACTATATAT 
GAGGGAGAAT TTTCAGATGA CTGGACTCTT AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGTATAAA AATCACAGGA 
ACCTACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACA GACCCAAAGT TTTGAAGCTG 
GGAAACCTGG CAGTGTCTGC TGATGAAAAG TGGAAAGCAG TATTTGATGA ATGTTGGCAT 
CAACTGGGCT GTGAGAACCC AGGCCAAGGG GAAGTTTGGA AAGCATGGGA CAATATTGCT 
GTGGCCCTGA CCACCAATCG GCGCCAACAC AGAGACAGTC CAGAGACATT AAGTCGTTCA 
CAGACTCAGA CACTAGAGAG TTTGGAATTC ATTCCTCAGC ATGTTGGTGC TTTCTCGGTA 
GAGAAATATG ATGACATCAA GAAATATTTA ATAAAGGCAT GTGACACTCC TCTGCACCCC 
CTGGGCAGGC TTGTGGAGAC ACTGGTTGCA GTTTATAGAA TGACATATGT GGGTGTAGGA 
GCCAATCGCC GGCTACTGCA GGAGGCTGTG AAAGAGATTA AATCTTACCT TAAGCGAATT 
TTCCAGCTGG TGAGATTCTT ATTTCCTGAG CTTCCTGAAG AGGGCAGCAC AATTCCTCTT 
TCTGCTCCTC TGCCAACGGA AAGGAAATCC TTTTGCACTG GGAAGTCAGA TTCCAGATCC 
GAATCACCAG AGCCAGGTTA TGTAGTAACA AGTTCTGGGT TACTGCTTCC TGTACTACTA 
CCTCGGCTCT ACCCACCACT GTTTATGCTC TATGCCCTGG ATAATGATCG TGAGGAAGAC 
ATTTACTGGG AGTGTGTTCT ACGACTAAAT AAGCAGCCAG ATATTGCTCT TCTCGGCTTC 
CTTGGGGTGC AGAGGAAATT TTGGCCAGTG ACCTTGTCAA TCCTTGGAGA GAGTAAAAAG 
GTTCTGCCAA CCACAAAAGA TGCTTGTTTT GCCTCAGCTG TAGAATGTCT ACAGCAGATC 
AGCACAACAT TTACCCCATC AGACAAACTT AAGGTCATCC AGCAGACTTT TGAAGAGATC 
TCTCAGAGTG TCCTGGCATC ACTTCAGGAA GACTTCCTGT GGTCCATGGA TGACTTGTTT 
CCTGTTTTCT TATATGTGGT GCTACGGGCC AGGATTAGGA ATTTAGGCTC TGAGGTACAC 
CTCATTGAAG ATCTAATGGA TCCATATTTA CAGCATGGGG AACAGGGTAT AATGTTCACC 
ACCTTAAAGG CATGTTACTA CCAGATCCAG CGTGAGAAGC TTAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_003640186.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003640234.1 Canis lupus familiaris ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X4, mRNA.

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CloneID	OCe31363
Clone ID Related Accession (Same CDS sequence)	XM_003640186.4 , XM_003640186.3 , XM_005640545.2
Accession Version	XM_003640186.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	alsin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006619.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003640186.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AAGCAGGATC CTATTCTGTA ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCAGGCAGC CCTTGGAGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGAAC CGGCAGAGAT TTGTCCGAGT 
TGCCCTATTC TAGAAAATGC CCTGATAGGG CAATTTGTCG TTACTGTGGC ATCAGGGAGT 
TTCCACAATG GAGCAGTAAC AGAAAGTGGT GTAGCATACA TGTGGGGGGA GAACTCTGCA 
GGCCAGTGTG CAGTAGCTAA CCAGCAGTAT GTTCCGGAAC CAAATCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTTCTTT GTTAGCAGTC AGGATTTTGC AGTTGGCATG TGGTGAGGAG 
CACACACTGG CATTATCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG 
GGTCTCATTA CCACTACCTT CCCGGTGACA AAGCCACAAA AGGTGGAACA TCTTGCTGGG 
CGAGTAGTGC TACAAGTTGC CTGTGGTGCG TTCCACAGCT TAGCACTTGT ACAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA AGATGCAACC AGTGCAGCCA ACTCTTGATT 
ACCATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCACTGTTG CCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAAAC CAGGCCAGCA CTGCCATCAG CCCCTCCACT 
GAAACCCTTG ACAATCAAGA AGTATTCGAG AACACGCTTG TAGAAAATGA GCTGCCTGTT 
GCTACTGATA CAAACGTTGG AAATGTTCAG ACCACGGGTG GTGATGCCAT ATCCTCCCAA 
CAAGAAATCA TGGGAACAAC TGAAATTCCT TCTGCCCAAA ATATACCATC ATACCCTGAT 
ACTCAGGCAG TAAATGAGTA CATGCAAAAA CTGTCAGATC ATTCAGTAAG AGACAACTCA 
GAGAACGGCA AAAAGCCAAT GCCATCTCAG CCTCTTGTAG AAGAAGCAGT TCCTAATCTT 
CACAGTCCAC CAACCACAAG CACCTCAGCC CTAAACAGCC TAGTAGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAGTACGGC CCCTTGTGAA CCTGGAACTC AGGCAGGCAG TAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAATC AATGCAAGGA 
AAGAAAAGTT CAAGTCTCGT GGATATCCGA GAAGAGGAAT CAGAGGGAGG CAGCCGAAGA 
CTCTCCCTCC CTGGATTGTT GTCCCAAGTT TCCCCCAGGC TCTTAAGAAA AGCTGCTCGG 
GTAAAAACTC GGACAGTGGT TCTGACACCC ACATACAGCG GAGAAGCAGA TGCACTCCTG 
CCTTCTCTTA GAACAGAAGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGTCAT 
GGTGATGTTC TGCCAAGGCT TCAACCACTG TGTGTAAAAT GTCTGGATGG TAAAGAAGTA 
ATCTATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCGAAATC CCAGGTTTAC 
TCATGGGGTA GCAATACCTT TGGTCAACTT GGACATTATG ATTTTCCAAC AACAGTTCCT 
CGTCTTGCAA AGGTGAGCAG TGAAAATGGA GTCTGGAGTG TCGCTGCCGG CCAGGATTAT 
TCCCTGTTCT TAGTGGATAC AGAAGACTTC CAGCCTGGGT TGTATTACAG TGGCCGACAG 
GACCCTGCGG AAGGTGACAA CCTTCCAGAG AATCACAGTG GCACTAAGAC TCCAGTACTT 
CTCTCCTGTA GTAAGCTTGG ATATATAAGT AGAGTGACAG CAGGAAAAGA TAGCTATCTA 
GCTTTGGTGG ACAAGAATGT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACCACG 
GAAAGACGGT TCTATGCAAA ACTAAGTGAC ATCAAATCTC AGATTCTCAG GCCTCTTCTC 
AGTTTAGAAA ATTTGGGTAC TGCAAGCACA GTCCAGCTGT TGCAGGAGGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAGCCT CACTGAGCAG CTTCCTTCAT 
GGGATAAAGG AAGCCAGGAG TTTGGTCATT CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAACTTCTT 
GCTAAGCCTG CCATTGATTT CCTAAATAAA AACCAGGAGC TATTACAAGA TTTATCAGAA 
GTAAATGATG AAAACACCCA ATTGATGGAA ATACTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGACTTC ATAATTATGC AAAAGTTTTG CTAAAGCTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAACT GCAGGATTCC AGTTCTTGTT ATGAGTGTCT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCTGAATAC ACCCTGGGCT TCTGGAAGAC CTTTCCTGGG 
AAAATGACGG ATTCCTTAAG GAAGCCAGAG CGTCGACTGC TCTGTGAAAG CAGTAACCGG 
GCCCTATCTC TGCAGCATGC TGGGAGGTTT TCTGTGAATT GGTTCATTCT CTTTAATGAT 
GCCCTGGTCC ATGCCCAGTT CTCCACACAT CATGTTTTCC CTTTGGCCAC ACTATGGGCA 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCTTAA AGATAACTAC ACCTGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACTA AGTGGCTTCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGACA GCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCCATTTCA CGCAGTGCCA AGTATACTTT CTATAAGGAT 
CCTCGCCTAA AAGATGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AATGGCCTGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATATG GAGAATACAG AATCCCAAAC AAAGCATTGA ACAAAGAAGA CCATTATGTA 
GGCCATTGGA AAGAAGGAAA AATGTGCGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAACATG CGTCATGGTC ATGGTCTCCT TCGAAGCGGA 
AAATTAACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACTAGA GGGGAAAAGT ATATGGGAAT GTGGCAAGAT 
GACGTATGTC AAGGGAATGG TGTGGTAGTT ACCCAATTTG GATTATACTA TGAAGGCAAC 
TTTCACCTTA ATAAAATGAT GGGAAATGGT GTTTTACTTT CTGAAGATGA TACTATATAT 
GAGGGAGAAT TTTCAGATGA CTGGACTCTT AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGTATAAA AATCACAGGA 
ACCTACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACA GACCCAAAGT TTTCAGGAAG 
CTGGGAAACC TGGCAGTGTC TGCTGATGAA AAGTGGAAAG CAGTATTTGA TGAATGTTGG 
CATCAACTGG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCATG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAC ATTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCTTTCTCG 
GTAGAGAAAT ATGATGACAT CAAGAAATAT TTAATAAAGG CATGTGACAC TCCTCTGCAC 
CCCCTGGGCA GGCTTGTGGA GACACTGGTT GCAGTTTATA GAATGACATA TGTGGGTGTA 
GGAGCCAATC GCCGGCTACT GCAGGAGGCT GTGAAAGAGA TTAAATCTTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGATT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTTTCTGCTC CTCTGCCAAC GGAAAGGAAA TCCTTTTGCA CTGGGAAGTC AGATTCCAGA 
TCCGAATCAC CAGAGCCAGG TTATGTAGTA ACAAGTTCTG GGTTACTGCT TCCTGTACTA 
CTACCTCGGC TCTACCCACC ACTGTTTATG CTCTATGCCC TGGATAATGA TCGTGAGGAA 
GACATTTACT GGGAGTGTGT TCTACGACTA AATAAGCAGC CAGATATTGC TCTTCTCGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAATCCTTGG AGAGAGTAAA 
AAGGTTCTGC CAACCACAAA AGATGCTTGT TTTGCCTCAG CTGTAGAATG TCTACAGCAG 
ATCAGCACAA CATTTACCCC ATCAGACAAA CTTAAGGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TGTGGTCCAT GGATGACTTG 
TTTCCTGTTT TCTTATATGT GGTGCTACGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATTG AAGATCTAAT GGATCCATAT TTACAGCATG GGGAACAGGG TATAATGTTC 
ACCACCTTAA AGGCATGTTA CTACCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003640234.1
CDS	213..5183
Translation	MDSKKRSSTEAEGSKERGLVHVWQAGSYSVTPERLPGWGGKTVLQAALGVKHGVLLTEDGEVYSFGTLPWRSEPAEICPSCPILENALIGQFVVTVASGSFHNGAVTESGVAYMWGENSAGQCAVANQQYVPEPNPVSISDSETSSLLAVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAENQASTAISPSTETLDNQEVFENTLVENELPVATDTNVGNVQTTGGDAISSQQEIMGTTEIPSAQNIPSYPDTQAVNEYMQKLSDHSVRDNSENGKKPMPSQPLVEEAVPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSTAPCEPGTQAGSSSIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIYLEAGGYHSLALTAKSQVYSWGSNTFGQLGHYDFPTTVPRLAKVSSENGVWSVAAGQDYSLFLVDTEDFQPGLYYSGRQDPAEGDNLPENHSGTKTPVLLSCSKLGYISRVTAGKDSYLALVDKNVMGYIASLHELATTERRFYAKLSDIKSQILRPLLSLENLGTASTVQLLQEVASRFSKLCYLIGQHGASLSSFLHGIKEARSLVILKHASLFLDSYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVNDENTQLMEILNTLFFLPIRRLHNYAKVLLKLATCFEVTSPEYQKLQDSSSCYECLALHLGRKRKEAEYTLGFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGTADLPPYGSGSSIQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGMWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKVFRKLGNLAVSADEKWKAVFDECWHQLGCENPGQGEVWKAWDNIAVALTTNRRQHRDSPETLSRSQTQTLESLEFIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALDNDREEDIYWECVLRLNKQPDIALLGFLGVQRKFWPVTLSILGESKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_003640186.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003640186.4

ORF Insert Sequence:

ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AAGCAGGATC CTATTCTGTA ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCAGGCAGC CCTTGGAGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGAAC CGGCAGAGAT TTGTCCGAGT 
TGCCCTATTC TAGAAAATGC CCTGATAGGG CAATTTGTCG TTACTGTGGC ATCAGGGAGT 
TTCCACAATG GAGCAGTAAC AGAAAGTGGT GTAGCATACA TGTGGGGGGA GAACTCTGCA 
GGCCAGTGTG CAGTAGCTAA CCAGCAGTAT GTTCCGGAAC CAAATCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTTCTTT GTTAGCAGTC AGGATTTTGC AGTTGGCATG TGGTGAGGAG 
CACACACTGG CATTATCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG 
GGTCTCATTA CCACTACCTT CCCGGTGACA AAGCCACAAA AGGTGGAACA TCTTGCTGGG 
CGAGTAGTGC TACAAGTTGC CTGTGGTGCG TTCCACAGCT TAGCACTTGT ACAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA AGATGCAACC AGTGCAGCCA ACTCTTGATT 
ACCATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCACTGTTG CCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAAAC CAGGCCAGCA CTGCCATCAG CCCCTCCACT 
GAAACCCTTG ACAATCAAGA AGTATTCGAG AACACGCTTG TAGAAAATGA GCTGCCTGTT 
GCTACTGATA CAAACGTTGG AAATGTTCAG ACCACGGGTG GTGATGCCAT ATCCTCCCAA 
CAAGAAATCA TGGGAACAAC TGAAATTCCT TCTGCCCAAA ATATACCATC ATACCCTGAT 
ACTCAGGCAG TAAATGAGTA CATGCAAAAA CTGTCAGATC ATTCAGTAAG AGACAACTCA 
GAGAACGGCA AAAAGCCAAT GCCATCTCAG CCTCTTGTAG AAGAAGCAGT TCCTAATCTT 
CACAGTCCAC CAACCACAAG CACCTCAGCC CTAAACAGCC TAGTAGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAGTACGGC CCCTTGTGAA CCTGGAACTC AGGCAGGCAG TAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAATC AATGCAAGGA 
AAGAAAAGTT CAAGTCTCGT GGATATCCGA GAAGAGGAAT CAGAGGGAGG CAGCCGAAGA 
CTCTCCCTCC CTGGATTGTT GTCCCAAGTT TCCCCCAGGC TCTTAAGAAA AGCTGCTCGG 
GTAAAAACTC GGACAGTGGT TCTGACACCC ACATACAGCG GAGAAGCAGA TGCACTCCTG 
CCTTCTCTTA GAACAGAAGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGTCAT 
GGTGATGTTC TGCCAAGGCT TCAACCACTG TGTGTAAAAT GTCTGGATGG TAAAGAAGTA 
ATCTATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCGAAATC CCAGGTTTAC 
TCATGGGGTA GCAATACCTT TGGTCAACTT GGACATTATG ATTTTCCAAC AACAGTTCCT 
CGTCTTGCAA AGGTGAGCAG TGAAAATGGA GTCTGGAGTG TCGCTGCCGG CCAGGATTAT 
TCCCTGTTCT TAGTGGATAC AGAAGACTTC CAGCCTGGGT TGTATTACAG TGGCCGACAG 
GACCCTGCGG AAGGTGACAA CCTTCCAGAG AATCACAGTG GCACTAAGAC TCCAGTACTT 
CTCTCCTGTA GTAAGCTTGG ATATATAAGT AGAGTGACAG CAGGAAAAGA TAGCTATCTA 
GCTTTGGTGG ACAAGAATGT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACCACG 
GAAAGACGGT TCTATGCAAA ACTAAGTGAC ATCAAATCTC AGATTCTCAG GCCTCTTCTC 
AGTTTAGAAA ATTTGGGTAC TGCAAGCACA GTCCAGCTGT TGCAGGAGGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAGCCT CACTGAGCAG CTTCCTTCAT 
GGGATAAAGG AAGCCAGGAG TTTGGTCATT CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAACTTCTT 
GCTAAGCCTG CCATTGATTT CCTAAATAAA AACCAGGAGC TATTACAAGA TTTATCAGAA 
GTAAATGATG AAAACACCCA ATTGATGGAA ATACTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGACTTC ATAATTATGC AAAAGTTTTG CTAAAGCTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAACT GCAGGATTCC AGTTCTTGTT ATGAGTGTCT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCTGAATAC ACCCTGGGCT TCTGGAAGAC CTTTCCTGGG 
AAAATGACGG ATTCCTTAAG GAAGCCAGAG CGTCGACTGC TCTGTGAAAG CAGTAACCGG 
GCCCTATCTC TGCAGCATGC TGGGAGGTTT TCTGTGAATT GGTTCATTCT CTTTAATGAT 
GCCCTGGTCC ATGCCCAGTT CTCCACACAT CATGTTTTCC CTTTGGCCAC ACTATGGGCA 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCTTAA AGATAACTAC ACCTGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACTA AGTGGCTTCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGACA GCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCCATTTCA CGCAGTGCCA AGTATACTTT CTATAAGGAT 
CCTCGCCTAA AAGATGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AATGGCCTGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATATG GAGAATACAG AATCCCAAAC AAAGCATTGA ACAAAGAAGA CCATTATGTA 
GGCCATTGGA AAGAAGGAAA AATGTGCGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAACATG CGTCATGGTC ATGGTCTCCT TCGAAGCGGA 
AAATTAACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACTAGA GGGGAAAAGT ATATGGGAAT GTGGCAAGAT 
GACGTATGTC AAGGGAATGG TGTGGTAGTT ACCCAATTTG GATTATACTA TGAAGGCAAC 
TTTCACCTTA ATAAAATGAT GGGAAATGGT GTTTTACTTT CTGAAGATGA TACTATATAT 
GAGGGAGAAT TTTCAGATGA CTGGACTCTT AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGTATAAA AATCACAGGA 
ACCTACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACA GACCCAAAGT TTTCAGGAAG 
CTGGGAAACC TGGCAGTGTC TGCTGATGAA AAGTGGAAAG CAGTATTTGA TGAATGTTGG 
CATCAACTGG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCATG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAC ATTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCTTTCTCG 
GTAGAGAAAT ATGATGACAT CAAGAAATAT TTAATAAAGG CATGTGACAC TCCTCTGCAC 
CCCCTGGGCA GGCTTGTGGA GACACTGGTT GCAGTTTATA GAATGACATA TGTGGGTGTA 
GGAGCCAATC GCCGGCTACT GCAGGAGGCT GTGAAAGAGA TTAAATCTTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGATT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTTTCTGCTC CTCTGCCAAC GGAAAGGAAA TCCTTTTGCA CTGGGAAGTC AGATTCCAGA 
TCCGAATCAC CAGAGCCAGG TTATGTAGTA ACAAGTTCTG GGTTACTGCT TCCTGTACTA 
CTACCTCGGC TCTACCCACC ACTGTTTATG CTCTATGCCC TGGATAATGA TCGTGAGGAA 
GACATTTACT GGGAGTGTGT TCTACGACTA AATAAGCAGC CAGATATTGC TCTTCTCGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAATCCTTGG AGAGAGTAAA 
AAGGTTCTGC CAACCACAAA AGATGCTTGT TTTGCCTCAG CTGTAGAATG TCTACAGCAG 
ATCAGCACAA CATTTACCCC ATCAGACAAA CTTAAGGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TGTGGTCCAT GGATGACTTG 
TTTCCTGTTT TCTTATATGT GGTGCTACGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATTG AAGATCTAAT GGATCCATAT TTACAGCATG GGGAACAGGG TATAATGTTC 
ACCACCTTAA AGGCATGTTA CTACCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_003640186.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003640234.1 Canis lupus familiaris amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X1, mRNA.

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CloneID	OCe31363
Clone ID Related Accession (Same CDS sequence)	XM_003640186.4 , XM_003640186.3 , XM_005640545.2
Accession Version	XM_003640186.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	alsin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726123.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003640186.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AAGCAGGATC CTATTCTGTA ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCAGGCAGC CCTTGGAGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGAAC CGGCAGAGAT TTGTCCGAGT 
TGCCCTATTC TAGAAAATGC CCTGATAGGG CAATTTGTCG TTACTGTGGC ATCAGGGAGT 
TTCCACAATG GAGCAGTAAC AGAAAGTGGT GTAGCATACA TGTGGGGGGA GAACTCTGCA 
GGCCAGTGTG CAGTAGCTAA CCAGCAGTAT GTTCCGGAAC CAAATCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTTCTTT GTTAGCAGTC AGGATTTTGC AGTTGGCATG TGGTGAGGAG 
CACACACTGG CATTATCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG 
GGTCTCATTA CCACTACCTT CCCGGTGACA AAGCCACAAA AGGTGGAACA TCTTGCTGGG 
CGAGTAGTGC TACAAGTTGC CTGTGGTGCG TTCCACAGCT TAGCACTTGT ACAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA AGATGCAACC AGTGCAGCCA ACTCTTGATT 
ACCATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCACTGTTG CCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAAAC CAGGCCAGCA CTGCCATCAG CCCCTCCACT 
GAAACCCTTG ACAATCAAGA AGTATTCGAG AACACGCTTG TAGAAAATGA GCTGCCTGTT 
GCTACTGATA CAAACGTTGG AAATGTTCAG ACCACGGGTG GTGATGCCAT ATCCTCCCAA 
CAAGAAATCA TGGGAACAAC TGAAATTCCT TCTGCCCAAA ATATACCATC ATACCCTGAT 
ACTCAGGCAG TAAATGAGTA CATGCAAAAA CTGTCAGATC ATTCAGTAAG AGACAACTCA 
GAGAACGGCA AAAAGCCAAT GCCATCTCAG CCTCTTGTAG AAGAAGCAGT TCCTAATCTT 
CACAGTCCAC CAACCACAAG CACCTCAGCC CTAAACAGCC TAGTAGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAGTACGGC CCCTTGTGAA CCTGGAACTC AGGCAGGCAG TAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAATC AATGCAAGGA 
AAGAAAAGTT CAAGTCTCGT GGATATCCGA GAAGAGGAAT CAGAGGGAGG CAGCCGAAGA 
CTCTCCCTCC CTGGATTGTT GTCCCAAGTT TCCCCCAGGC TCTTAAGAAA AGCTGCTCGG 
GTAAAAACTC GGACAGTGGT TCTGACACCC ACATACAGCG GAGAAGCAGA TGCACTCCTG 
CCTTCTCTTA GAACAGAAGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGTCAT 
GGTGATGTTC TGCCAAGGCT TCAACCACTG TGTGTAAAAT GTCTGGATGG TAAAGAAGTA 
ATCTATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCGAAATC CCAGGTTTAC 
TCATGGGGTA GCAATACCTT TGGTCAACTT GGACATTATG ATTTTCCAAC AACAGTTCCT 
CGTCTTGCAA AGGTGAGCAG TGAAAATGGA GTCTGGAGTG TCGCTGCCGG CCAGGATTAT 
TCCCTGTTCT TAGTGGATAC AGAAGACTTC CAGCCTGGGT TGTATTACAG TGGCCGACAG 
GACCCTGCGG AAGGTGACAA CCTTCCAGAG AATCACAGTG GCACTAAGAC TCCAGTACTT 
CTCTCCTGTA GTAAGCTTGG ATATATAAGT AGAGTGACAG CAGGAAAAGA TAGCTATCTA 
GCTTTGGTGG ACAAGAATGT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACCACG 
GAAAGACGGT TCTATGCAAA ACTAAGTGAC ATCAAATCTC AGATTCTCAG GCCTCTTCTC 
AGTTTAGAAA ATTTGGGTAC TGCAAGCACA GTCCAGCTGT TGCAGGAGGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAGCCT CACTGAGCAG CTTCCTTCAT 
GGGATAAAGG AAGCCAGGAG TTTGGTCATT CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAACTTCTT 
GCTAAGCCTG CCATTGATTT CCTAAATAAA AACCAGGAGC TATTACAAGA TTTATCAGAA 
GTAAATGATG AAAACACCCA ATTGATGGAA ATACTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGACTTC ATAATTATGC AAAAGTTTTG CTAAAGCTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAACT GCAGGATTCC AGTTCTTGTT ATGAGTGTCT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCTGAATAC ACCCTGGGCT TCTGGAAGAC CTTTCCTGGG 
AAAATGACGG ATTCCTTAAG GAAGCCAGAG CGTCGACTGC TCTGTGAAAG CAGTAACCGG 
GCCCTATCTC TGCAGCATGC TGGGAGGTTT TCTGTGAATT GGTTCATTCT CTTTAATGAT 
GCCCTGGTCC ATGCCCAGTT CTCCACACAT CATGTTTTCC CTTTGGCCAC ACTATGGGCA 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCTTAA AGATAACTAC ACCTGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACTA AGTGGCTTCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGACA GCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCCATTTCA CGCAGTGCCA AGTATACTTT CTATAAGGAT 
CCTCGCCTAA AAGATGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AATGGCCTGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATATG GAGAATACAG AATCCCAAAC AAAGCATTGA ACAAAGAAGA CCATTATGTA 
GGCCATTGGA AAGAAGGAAA AATGTGCGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAACATG CGTCATGGTC ATGGTCTCCT TCGAAGCGGA 
AAATTAACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACTAGA GGGGAAAAGT ATATGGGAAT GTGGCAAGAT 
GACGTATGTC AAGGGAATGG TGTGGTAGTT ACCCAATTTG GATTATACTA TGAAGGCAAC 
TTTCACCTTA ATAAAATGAT GGGAAATGGT GTTTTACTTT CTGAAGATGA TACTATATAT 
GAGGGAGAAT TTTCAGATGA CTGGACTCTT AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGTATAAA AATCACAGGA 
ACCTACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACA GACCCAAAGT TTTCAGGAAG 
CTGGGAAACC TGGCAGTGTC TGCTGATGAA AAGTGGAAAG CAGTATTTGA TGAATGTTGG 
CATCAACTGG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCATG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAC ATTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCTTTCTCG 
GTAGAGAAAT ATGATGACAT CAAGAAATAT TTAATAAAGG CATGTGACAC TCCTCTGCAC 
CCCCTGGGCA GGCTTGTGGA GACACTGGTT GCAGTTTATA GAATGACATA TGTGGGTGTA 
GGAGCCAATC GCCGGCTACT GCAGGAGGCT GTGAAAGAGA TTAAATCTTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGATT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTTTCTGCTC CTCTGCCAAC GGAAAGGAAA TCCTTTTGCA CTGGGAAGTC AGATTCCAGA 
TCCGAATCAC CAGAGCCAGG TTATGTAGTA ACAAGTTCTG GGTTACTGCT TCCTGTACTA 
CTACCTCGGC TCTACCCACC ACTGTTTATG CTCTATGCCC TGGATAATGA TCGTGAGGAA 
GACATTTACT GGGAGTGTGT TCTACGACTA AATAAGCAGC CAGATATTGC TCTTCTCGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAATCCTTGG AGAGAGTAAA 
AAGGTTCTGC CAACCACAAA AGATGCTTGT TTTGCCTCAG CTGTAGAATG TCTACAGCAG 
ATCAGCACAA CATTTACCCC ATCAGACAAA CTTAAGGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TGTGGTCCAT GGATGACTTG 
TTTCCTGTTT TCTTATATGT GGTGCTACGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATTG AAGATCTAAT GGATCCATAT TTACAGCATG GGGAACAGGG TATAATGTTC 
ACCACCTTAA AGGCATGTTA CTACCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003640234.1
CDS	154..5124
Translation	MDSKKRSSTEAEGSKERGLVHVWQAGSYSVTPERLPGWGGKTVLQAALGVKHGVLLTEDGEVYSFGTLPWRSEPAEICPSCPILENALIGQFVVTVASGSFHNGAVTESGVAYMWGENSAGQCAVANQQYVPEPNPVSISDSETSSLLAVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAENQASTAISPSTETLDNQEVFENTLVENELPVATDTNVGNVQTTGGDAISSQQEIMGTTEIPSAQNIPSYPDTQAVNEYMQKLSDHSVRDNSENGKKPMPSQPLVEEAVPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSTAPCEPGTQAGSSSIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIYLEAGGYHSLALTAKSQVYSWGSNTFGQLGHYDFPTTVPRLAKVSSENGVWSVAAGQDYSLFLVDTEDFQPGLYYSGRQDPAEGDNLPENHSGTKTPVLLSCSKLGYISRVTAGKDSYLALVDKNVMGYIASLHELATTERRFYAKLSDIKSQILRPLLSLENLGTASTVQLLQEVASRFSKLCYLIGQHGASLSSFLHGIKEARSLVILKHASLFLDSYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVNDENTQLMEILNTLFFLPIRRLHNYAKVLLKLATCFEVTSPEYQKLQDSSSCYECLALHLGRKRKEAEYTLGFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGTADLPPYGSGSSIQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGMWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKVFRKLGNLAVSADEKWKAVFDECWHQLGCENPGQGEVWKAWDNIAVALTTNRRQHRDSPETLSRSQTQTLESLEFIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALDNDREEDIYWECVLRLNKQPDIALLGFLGVQRKFWPVTLSILGESKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_003640186.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003640186.3

ORF Insert Sequence:

ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AAGCAGGATC CTATTCTGTA ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCAGGCAGC CCTTGGAGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGAAC CGGCAGAGAT TTGTCCGAGT 
TGCCCTATTC TAGAAAATGC CCTGATAGGG CAATTTGTCG TTACTGTGGC ATCAGGGAGT 
TTCCACAATG GAGCAGTAAC AGAAAGTGGT GTAGCATACA TGTGGGGGGA GAACTCTGCA 
GGCCAGTGTG CAGTAGCTAA CCAGCAGTAT GTTCCGGAAC CAAATCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTTCTTT GTTAGCAGTC AGGATTTTGC AGTTGGCATG TGGTGAGGAG 
CACACACTGG CATTATCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG 
GGTCTCATTA CCACTACCTT CCCGGTGACA AAGCCACAAA AGGTGGAACA TCTTGCTGGG 
CGAGTAGTGC TACAAGTTGC CTGTGGTGCG TTCCACAGCT TAGCACTTGT ACAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA AGATGCAACC AGTGCAGCCA ACTCTTGATT 
ACCATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCACTGTTG CCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAAAC CAGGCCAGCA CTGCCATCAG CCCCTCCACT 
GAAACCCTTG ACAATCAAGA AGTATTCGAG AACACGCTTG TAGAAAATGA GCTGCCTGTT 
GCTACTGATA CAAACGTTGG AAATGTTCAG ACCACGGGTG GTGATGCCAT ATCCTCCCAA 
CAAGAAATCA TGGGAACAAC TGAAATTCCT TCTGCCCAAA ATATACCATC ATACCCTGAT 
ACTCAGGCAG TAAATGAGTA CATGCAAAAA CTGTCAGATC ATTCAGTAAG AGACAACTCA 
GAGAACGGCA AAAAGCCAAT GCCATCTCAG CCTCTTGTAG AAGAAGCAGT TCCTAATCTT 
CACAGTCCAC CAACCACAAG CACCTCAGCC CTAAACAGCC TAGTAGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAGTACGGC CCCTTGTGAA CCTGGAACTC AGGCAGGCAG TAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAATC AATGCAAGGA 
AAGAAAAGTT CAAGTCTCGT GGATATCCGA GAAGAGGAAT CAGAGGGAGG CAGCCGAAGA 
CTCTCCCTCC CTGGATTGTT GTCCCAAGTT TCCCCCAGGC TCTTAAGAAA AGCTGCTCGG 
GTAAAAACTC GGACAGTGGT TCTGACACCC ACATACAGCG GAGAAGCAGA TGCACTCCTG 
CCTTCTCTTA GAACAGAAGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGTCAT 
GGTGATGTTC TGCCAAGGCT TCAACCACTG TGTGTAAAAT GTCTGGATGG TAAAGAAGTA 
ATCTATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCGAAATC CCAGGTTTAC 
TCATGGGGTA GCAATACCTT TGGTCAACTT GGACATTATG ATTTTCCAAC AACAGTTCCT 
CGTCTTGCAA AGGTGAGCAG TGAAAATGGA GTCTGGAGTG TCGCTGCCGG CCAGGATTAT 
TCCCTGTTCT TAGTGGATAC AGAAGACTTC CAGCCTGGGT TGTATTACAG TGGCCGACAG 
GACCCTGCGG AAGGTGACAA CCTTCCAGAG AATCACAGTG GCACTAAGAC TCCAGTACTT 
CTCTCCTGTA GTAAGCTTGG ATATATAAGT AGAGTGACAG CAGGAAAAGA TAGCTATCTA 
GCTTTGGTGG ACAAGAATGT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACCACG 
GAAAGACGGT TCTATGCAAA ACTAAGTGAC ATCAAATCTC AGATTCTCAG GCCTCTTCTC 
AGTTTAGAAA ATTTGGGTAC TGCAAGCACA GTCCAGCTGT TGCAGGAGGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAGCCT CACTGAGCAG CTTCCTTCAT 
GGGATAAAGG AAGCCAGGAG TTTGGTCATT CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAACTTCTT 
GCTAAGCCTG CCATTGATTT CCTAAATAAA AACCAGGAGC TATTACAAGA TTTATCAGAA 
GTAAATGATG AAAACACCCA ATTGATGGAA ATACTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGACTTC ATAATTATGC AAAAGTTTTG CTAAAGCTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAACT GCAGGATTCC AGTTCTTGTT ATGAGTGTCT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCTGAATAC ACCCTGGGCT TCTGGAAGAC CTTTCCTGGG 
AAAATGACGG ATTCCTTAAG GAAGCCAGAG CGTCGACTGC TCTGTGAAAG CAGTAACCGG 
GCCCTATCTC TGCAGCATGC TGGGAGGTTT TCTGTGAATT GGTTCATTCT CTTTAATGAT 
GCCCTGGTCC ATGCCCAGTT CTCCACACAT CATGTTTTCC CTTTGGCCAC ACTATGGGCA 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCTTAA AGATAACTAC ACCTGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACTA AGTGGCTTCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGACA GCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCCATTTCA CGCAGTGCCA AGTATACTTT CTATAAGGAT 
CCTCGCCTAA AAGATGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AATGGCCTGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATATG GAGAATACAG AATCCCAAAC AAAGCATTGA ACAAAGAAGA CCATTATGTA 
GGCCATTGGA AAGAAGGAAA AATGTGCGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAACATG CGTCATGGTC ATGGTCTCCT TCGAAGCGGA 
AAATTAACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACTAGA GGGGAAAAGT ATATGGGAAT GTGGCAAGAT 
GACGTATGTC AAGGGAATGG TGTGGTAGTT ACCCAATTTG GATTATACTA TGAAGGCAAC 
TTTCACCTTA ATAAAATGAT GGGAAATGGT GTTTTACTTT CTGAAGATGA TACTATATAT 
GAGGGAGAAT TTTCAGATGA CTGGACTCTT AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGTATAAA AATCACAGGA 
ACCTACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACA GACCCAAAGT TTTCAGGAAG 
CTGGGAAACC TGGCAGTGTC TGCTGATGAA AAGTGGAAAG CAGTATTTGA TGAATGTTGG 
CATCAACTGG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCATG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAC ATTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCTTTCTCG 
GTAGAGAAAT ATGATGACAT CAAGAAATAT TTAATAAAGG CATGTGACAC TCCTCTGCAC 
CCCCTGGGCA GGCTTGTGGA GACACTGGTT GCAGTTTATA GAATGACATA TGTGGGTGTA 
GGAGCCAATC GCCGGCTACT GCAGGAGGCT GTGAAAGAGA TTAAATCTTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGATT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTTTCTGCTC CTCTGCCAAC GGAAAGGAAA TCCTTTTGCA CTGGGAAGTC AGATTCCAGA 
TCCGAATCAC CAGAGCCAGG TTATGTAGTA ACAAGTTCTG GGTTACTGCT TCCTGTACTA 
CTACCTCGGC TCTACCCACC ACTGTTTATG CTCTATGCCC TGGATAATGA TCGTGAGGAA 
GACATTTACT GGGAGTGTGT TCTACGACTA AATAAGCAGC CAGATATTGC TCTTCTCGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAATCCTTGG AGAGAGTAAA 
AAGGTTCTGC CAACCACAAA AGATGCTTGT TTTGCCTCAG CTGTAGAATG TCTACAGCAG 
ATCAGCACAA CATTTACCCC ATCAGACAAA CTTAAGGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TGTGGTCCAT GGATGACTTG 
TTTCCTGTTT TCTTATATGT GGTGCTACGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATTG AAGATCTAAT GGATCCATAT TTACAGCATG GGGAACAGGG TATAATGTTC 
ACCACCTTAA AGGCATGTTA CTACCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_005640545.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005640602.1 Canis lupus familiaris amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X2, mRNA.

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CloneID	OCe31363
Clone ID Related Accession (Same CDS sequence)	XM_003640186.4 , XM_003640186.3 , XM_005640545.2
Accession Version	XM_005640545.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	alsin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726123.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640545.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AAGCAGGATC CTATTCTGTA ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCAGGCAGC CCTTGGAGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGAAC CGGCAGAGAT TTGTCCGAGT 
TGCCCTATTC TAGAAAATGC CCTGATAGGG CAATTTGTCG TTACTGTGGC ATCAGGGAGT 
TTCCACAATG GAGCAGTAAC AGAAAGTGGT GTAGCATACA TGTGGGGGGA GAACTCTGCA 
GGCCAGTGTG CAGTAGCTAA CCAGCAGTAT GTTCCGGAAC CAAATCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTTCTTT GTTAGCAGTC AGGATTTTGC AGTTGGCATG TGGTGAGGAG 
CACACACTGG CATTATCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG 
GGTCTCATTA CCACTACCTT CCCGGTGACA AAGCCACAAA AGGTGGAACA TCTTGCTGGG 
CGAGTAGTGC TACAAGTTGC CTGTGGTGCG TTCCACAGCT TAGCACTTGT ACAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA AGATGCAACC AGTGCAGCCA ACTCTTGATT 
ACCATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCACTGTTG CCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAAAC CAGGCCAGCA CTGCCATCAG CCCCTCCACT 
GAAACCCTTG ACAATCAAGA AGTATTCGAG AACACGCTTG TAGAAAATGA GCTGCCTGTT 
GCTACTGATA CAAACGTTGG AAATGTTCAG ACCACGGGTG GTGATGCCAT ATCCTCCCAA 
CAAGAAATCA TGGGAACAAC TGAAATTCCT TCTGCCCAAA ATATACCATC ATACCCTGAT 
ACTCAGGCAG TAAATGAGTA CATGCAAAAA CTGTCAGATC ATTCAGTAAG AGACAACTCA 
GAGAACGGCA AAAAGCCAAT GCCATCTCAG CCTCTTGTAG AAGAAGCAGT TCCTAATCTT 
CACAGTCCAC CAACCACAAG CACCTCAGCC CTAAACAGCC TAGTAGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAGTACGGC CCCTTGTGAA CCTGGAACTC AGGCAGGCAG TAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAATC AATGCAAGGA 
AAGAAAAGTT CAAGTCTCGT GGATATCCGA GAAGAGGAAT CAGAGGGAGG CAGCCGAAGA 
CTCTCCCTCC CTGGATTGTT GTCCCAAGTT TCCCCCAGGC TCTTAAGAAA AGCTGCTCGG 
GTAAAAACTC GGACAGTGGT TCTGACACCC ACATACAGCG GAGAAGCAGA TGCACTCCTG 
CCTTCTCTTA GAACAGAAGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGTCAT 
GGTGATGTTC TGCCAAGGCT TCAACCACTG TGTGTAAAAT GTCTGGATGG TAAAGAAGTA 
ATCTATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCGAAATC CCAGGTTTAC 
TCATGGGGTA GCAATACCTT TGGTCAACTT GGACATTATG ATTTTCCAAC AACAGTTCCT 
CGTCTTGCAA AGGTGAGCAG TGAAAATGGA GTCTGGAGTG TCGCTGCCGG CCAGGATTAT 
TCCCTGTTCT TAGTGGATAC AGAAGACTTC CAGCCTGGGT TGTATTACAG TGGCCGACAG 
GACCCTGCGG AAGGTGACAA CCTTCCAGAG AATCACAGTG GCACTAAGAC TCCAGTACTT 
CTCTCCTGTA GTAAGCTTGG ATATATAAGT AGAGTGACAG CAGGAAAAGA TAGCTATCTA 
GCTTTGGTGG ACAAGAATGT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACCACG 
GAAAGACGGT TCTATGCAAA ACTAAGTGAC ATCAAATCTC AGATTCTCAG GCCTCTTCTC 
AGTTTAGAAA ATTTGGGTAC TGCAAGCACA GTCCAGCTGT TGCAGGAGGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAGCCT CACTGAGCAG CTTCCTTCAT 
GGGATAAAGG AAGCCAGGAG TTTGGTCATT CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAACTTCTT 
GCTAAGCCTG CCATTGATTT CCTAAATAAA AACCAGGAGC TATTACAAGA TTTATCAGAA 
GTAAATGATG AAAACACCCA ATTGATGGAA ATACTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGACTTC ATAATTATGC AAAAGTTTTG CTAAAGCTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAACT GCAGGATTCC AGTTCTTGTT ATGAGTGTCT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCTGAATAC ACCCTGGGCT TCTGGAAGAC CTTTCCTGGG 
AAAATGACGG ATTCCTTAAG GAAGCCAGAG CGTCGACTGC TCTGTGAAAG CAGTAACCGG 
GCCCTATCTC TGCAGCATGC TGGGAGGTTT TCTGTGAATT GGTTCATTCT CTTTAATGAT 
GCCCTGGTCC ATGCCCAGTT CTCCACACAT CATGTTTTCC CTTTGGCCAC ACTATGGGCA 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCTTAA AGATAACTAC ACCTGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACTA AGTGGCTTCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGACA GCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCCATTTCA CGCAGTGCCA AGTATACTTT CTATAAGGAT 
CCTCGCCTAA AAGATGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AATGGCCTGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATATG GAGAATACAG AATCCCAAAC AAAGCATTGA ACAAAGAAGA CCATTATGTA 
GGCCATTGGA AAGAAGGAAA AATGTGCGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAACATG CGTCATGGTC ATGGTCTCCT TCGAAGCGGA 
AAATTAACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACTAGA GGGGAAAAGT ATATGGGAAT GTGGCAAGAT 
GACGTATGTC AAGGGAATGG TGTGGTAGTT ACCCAATTTG GATTATACTA TGAAGGCAAC 
TTTCACCTTA ATAAAATGAT GGGAAATGGT GTTTTACTTT CTGAAGATGA TACTATATAT 
GAGGGAGAAT TTTCAGATGA CTGGACTCTT AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGTATAAA AATCACAGGA 
ACCTACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACA GACCCAAAGT TTTCAGGAAG 
CTGGGAAACC TGGCAGTGTC TGCTGATGAA AAGTGGAAAG CAGTATTTGA TGAATGTTGG 
CATCAACTGG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCATG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAC ATTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCTTTCTCG 
GTAGAGAAAT ATGATGACAT CAAGAAATAT TTAATAAAGG CATGTGACAC TCCTCTGCAC 
CCCCTGGGCA GGCTTGTGGA GACACTGGTT GCAGTTTATA GAATGACATA TGTGGGTGTA 
GGAGCCAATC GCCGGCTACT GCAGGAGGCT GTGAAAGAGA TTAAATCTTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGATT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTTTCTGCTC CTCTGCCAAC GGAAAGGAAA TCCTTTTGCA CTGGGAAGTC AGATTCCAGA 
TCCGAATCAC CAGAGCCAGG TTATGTAGTA ACAAGTTCTG GGTTACTGCT TCCTGTACTA 
CTACCTCGGC TCTACCCACC ACTGTTTATG CTCTATGCCC TGGATAATGA TCGTGAGGAA 
GACATTTACT GGGAGTGTGT TCTACGACTA AATAAGCAGC CAGATATTGC TCTTCTCGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAATCCTTGG AGAGAGTAAA 
AAGGTTCTGC CAACCACAAA AGATGCTTGT TTTGCCTCAG CTGTAGAATG TCTACAGCAG 
ATCAGCACAA CATTTACCCC ATCAGACAAA CTTAAGGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TGTGGTCCAT GGATGACTTG 
TTTCCTGTTT TCTTATATGT GGTGCTACGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATTG AAGATCTAAT GGATCCATAT TTACAGCATG GGGAACAGGG TATAATGTTC 
ACCACCTTAA AGGCATGTTA CTACCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005640602.1
CDS	493..5463
Translation	MDSKKRSSTEAEGSKERGLVHVWQAGSYSVTPERLPGWGGKTVLQAALGVKHGVLLTEDGEVYSFGTLPWRSEPAEICPSCPILENALIGQFVVTVASGSFHNGAVTESGVAYMWGENSAGQCAVANQQYVPEPNPVSISDSETSSLLAVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAENQASTAISPSTETLDNQEVFENTLVENELPVATDTNVGNVQTTGGDAISSQQEIMGTTEIPSAQNIPSYPDTQAVNEYMQKLSDHSVRDNSENGKKPMPSQPLVEEAVPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSTAPCEPGTQAGSSSIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIYLEAGGYHSLALTAKSQVYSWGSNTFGQLGHYDFPTTVPRLAKVSSENGVWSVAAGQDYSLFLVDTEDFQPGLYYSGRQDPAEGDNLPENHSGTKTPVLLSCSKLGYISRVTAGKDSYLALVDKNVMGYIASLHELATTERRFYAKLSDIKSQILRPLLSLENLGTASTVQLLQEVASRFSKLCYLIGQHGASLSSFLHGIKEARSLVILKHASLFLDSYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVNDENTQLMEILNTLFFLPIRRLHNYAKVLLKLATCFEVTSPEYQKLQDSSSCYECLALHLGRKRKEAEYTLGFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGTADLPPYGSGSSIQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGMWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDRPKVFRKLGNLAVSADEKWKAVFDECWHQLGCENPGQGEVWKAWDNIAVALTTNRRQHRDSPETLSRSQTQTLESLEFIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALDNDREEDIYWECVLRLNKQPDIALLGFLGVQRKFWPVTLSILGESKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_005640545.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005640545.2

ORF Insert Sequence:

ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTGGTC 
CATGTGTGGC AAGCAGGATC CTATTCTGTA ACACCAGAGA GATTGCCAGG CTGGGGAGGA 
AAGACTGTTC TTCAGGCAGC CCTTGGAGTG AAGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGAAC CGGCAGAGAT TTGTCCGAGT 
TGCCCTATTC TAGAAAATGC CCTGATAGGG CAATTTGTCG TTACTGTGGC ATCAGGGAGT 
TTCCACAATG GAGCAGTAAC AGAAAGTGGT GTAGCATACA TGTGGGGGGA GAACTCTGCA 
GGCCAGTGTG CAGTAGCTAA CCAGCAGTAT GTTCCGGAAC CAAATCCTGT CAGCATTTCT 
GATTCTGAGA CCAGTTCTTT GTTAGCAGTC AGGATTTTGC AGTTGGCATG TGGTGAGGAG 
CACACACTGG CATTATCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG 
GGTCTCATTA CCACTACCTT CCCGGTGACA AAGCCACAAA AGGTGGAACA TCTTGCTGGG 
CGAGTAGTGC TACAAGTTGC CTGTGGTGCG TTCCACAGCT TAGCACTTGT ACAGTGCCTC 
CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA AGATGCAACC AGTGCAGCCA ACTCTTGATT 
ACCATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCACTGTTG CCCATTAGGT 
GTGACACTGT CAGAATCCCA GGCAGAAAAC CAGGCCAGCA CTGCCATCAG CCCCTCCACT 
GAAACCCTTG ACAATCAAGA AGTATTCGAG AACACGCTTG TAGAAAATGA GCTGCCTGTT 
GCTACTGATA CAAACGTTGG AAATGTTCAG ACCACGGGTG GTGATGCCAT ATCCTCCCAA 
CAAGAAATCA TGGGAACAAC TGAAATTCCT TCTGCCCAAA ATATACCATC ATACCCTGAT 
ACTCAGGCAG TAAATGAGTA CATGCAAAAA CTGTCAGATC ATTCAGTAAG AGACAACTCA 
GAGAACGGCA AAAAGCCAAT GCCATCTCAG CCTCTTGTAG AAGAAGCAGT TCCTAATCTT 
CACAGTCCAC CAACCACAAG CACCTCAGCC CTAAACAGCC TAGTAGTCTC TTGTGCATCT 
GCTGTTGGTG TGAGAGTGGC TGCTACATAT GAAGCTGGGG CCTTGTCTCT TAAGAAAGTT 
ATGAACTTTT ATAGTACGGC CCCTTGTGAA CCTGGAACTC AGGCAGGCAG TAGTTCCATA 
GGCCCAGAAG GTCTGAAAGA TAGCAGAGAA GAACAGGTTA AACAGGAATC AATGCAAGGA 
AAGAAAAGTT CAAGTCTCGT GGATATCCGA GAAGAGGAAT CAGAGGGAGG CAGCCGAAGA 
CTCTCCCTCC CTGGATTGTT GTCCCAAGTT TCCCCCAGGC TCTTAAGAAA AGCTGCTCGG 
GTAAAAACTC GGACAGTGGT TCTGACACCC ACATACAGCG GAGAAGCAGA TGCACTCCTG 
CCTTCTCTTA GAACAGAAGT GTGGACCTGG GGGAAAGGGA AGGAAGGACA GCTGGGTCAT 
GGTGATGTTC TGCCAAGGCT TCAACCACTG TGTGTAAAAT GTCTGGATGG TAAAGAAGTA 
ATCTATCTGG AGGCAGGTGG TTACCATTCT CTTGCACTTA CTGCGAAATC CCAGGTTTAC 
TCATGGGGTA GCAATACCTT TGGTCAACTT GGACATTATG ATTTTCCAAC AACAGTTCCT 
CGTCTTGCAA AGGTGAGCAG TGAAAATGGA GTCTGGAGTG TCGCTGCCGG CCAGGATTAT 
TCCCTGTTCT TAGTGGATAC AGAAGACTTC CAGCCTGGGT TGTATTACAG TGGCCGACAG 
GACCCTGCGG AAGGTGACAA CCTTCCAGAG AATCACAGTG GCACTAAGAC TCCAGTACTT 
CTCTCCTGTA GTAAGCTTGG ATATATAAGT AGAGTGACAG CAGGAAAAGA TAGCTATCTA 
GCTTTGGTGG ACAAGAATGT TATGGGATAT ATTGCCAGTC TCCATGAGTT GGCTACCACG 
GAAAGACGGT TCTATGCAAA ACTAAGTGAC ATCAAATCTC AGATTCTCAG GCCTCTTCTC 
AGTTTAGAAA ATTTGGGTAC TGCAAGCACA GTCCAGCTGT TGCAGGAGGT GGCAAGCCGA 
TTCAGCAAGC TGTGTTACCT AATTGGTCAG CATGGAGCCT CACTGAGCAG CTTCCTTCAT 
GGGATAAAGG AAGCCAGGAG TTTGGTCATT CTGAAGCATG CAAGTCTCTT CTTGGATAGT 
TATACAGAGT ATTGCACATC TATTACAAAT TTCCTGGTTA TGGGAGGATT CCAACTTCTT 
GCTAAGCCTG CCATTGATTT CCTAAATAAA AACCAGGAGC TATTACAAGA TTTATCAGAA 
GTAAATGATG AAAACACCCA ATTGATGGAA ATACTGAATA CTCTGTTTTT CTTGCCAATC 
AGACGACTTC ATAATTATGC AAAAGTTTTG CTAAAGCTTG CTACTTGTTT TGAAGTGACA 
TCTCCAGAAT ACCAGAAACT GCAGGATTCC AGTTCTTGTT ATGAGTGTCT TGCTCTCCAT 
CTCGGCAGGA AAAGGAAGGA AGCTGAATAC ACCCTGGGCT TCTGGAAGAC CTTTCCTGGG 
AAAATGACGG ATTCCTTAAG GAAGCCAGAG CGTCGACTGC TCTGTGAAAG CAGTAACCGG 
GCCCTATCTC TGCAGCATGC TGGGAGGTTT TCTGTGAATT GGTTCATTCT CTTTAATGAT 
GCCCTGGTCC ATGCCCAGTT CTCCACACAT CATGTTTTCC CTTTGGCCAC ACTATGGGCA 
GAGCCACTTT CTGAAGAAGC TGGTGGTGTG AATGGCTTAA AGATAACTAC ACCTGAGGAG 
CAGTTCACTC TCATTTCATC AACACCCCAG GAAAAGACTA AGTGGCTTCG GGCTATAAGC 
CAAGCTGTGG ATCAGGCTTT GAGGGGGACA GCTGATCTTC CACCTTATGG AAGTGGCAGC 
AGTATTCAGA GGCAGGAACC ACCCATTTCA CGCAGTGCCA AGTATACTTT CTATAAGGAT 
CCTCGCCTAA AAGATGCAAC CTATGATGGA CGCTGGCTTT CAGGGAAGCC TCATGGCAGA 
GGGGTTTTGA AATGGCCTGA TGGAAAGATG TATTCTGGCA TGTTCAGGAA TGGCTTGGAA 
GATGGATATG GAGAATACAG AATCCCAAAC AAAGCATTGA ACAAAGAAGA CCATTATGTA 
GGCCATTGGA AAGAAGGAAA AATGTGCGGT CAAGGAGTCT ATAGTTATGC CTCTGGTGAA 
GTGTTTGAAG GCTGTTTTCA AGATAACATG CGTCATGGTC ATGGTCTCCT TCGAAGCGGA 
AAATTAACTT CCTCTTCTCC CAGTATGTTC ATTGGCCAGT GGGTAATGGA TAAGAAAGCA 
GGATATGGTG TCTTTGATGA TATCACTAGA GGGGAAAAGT ATATGGGAAT GTGGCAAGAT 
GACGTATGTC AAGGGAATGG TGTGGTAGTT ACCCAATTTG GATTATACTA TGAAGGCAAC 
TTTCACCTTA ATAAAATGAT GGGAAATGGT GTTTTACTTT CTGAAGATGA TACTATATAT 
GAGGGAGAAT TTTCAGATGA CTGGACTCTT AGTGGAAAGG GAACACTGAC TATGCCAAAT 
GGAGATTACA TTGAAGGTTA TTTTAGTGGA GAATGGGGAT CTGGTATAAA AATCACAGGA 
ACCTACTTCA AGCCTAGTCT GTATGAGAGT GATAAAGACA GACCCAAAGT TTTCAGGAAG 
CTGGGAAACC TGGCAGTGTC TGCTGATGAA AAGTGGAAAG CAGTATTTGA TGAATGTTGG 
CATCAACTGG GCTGTGAGAA CCCAGGCCAA GGGGAAGTTT GGAAAGCATG GGACAATATT 
GCTGTGGCCC TGACCACCAA TCGGCGCCAA CACAGAGACA GTCCAGAGAC ATTAAGTCGT 
TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCTC AGCATGTTGG TGCTTTCTCG 
GTAGAGAAAT ATGATGACAT CAAGAAATAT TTAATAAAGG CATGTGACAC TCCTCTGCAC 
CCCCTGGGCA GGCTTGTGGA GACACTGGTT GCAGTTTATA GAATGACATA TGTGGGTGTA 
GGAGCCAATC GCCGGCTACT GCAGGAGGCT GTGAAAGAGA TTAAATCTTA CCTTAAGCGA 
ATTTTCCAGC TGGTGAGATT CTTATTTCCT GAGCTTCCTG AAGAGGGCAG CACAATTCCT 
CTTTCTGCTC CTCTGCCAAC GGAAAGGAAA TCCTTTTGCA CTGGGAAGTC AGATTCCAGA 
TCCGAATCAC CAGAGCCAGG TTATGTAGTA ACAAGTTCTG GGTTACTGCT TCCTGTACTA 
CTACCTCGGC TCTACCCACC ACTGTTTATG CTCTATGCCC TGGATAATGA TCGTGAGGAA 
GACATTTACT GGGAGTGTGT TCTACGACTA AATAAGCAGC CAGATATTGC TCTTCTCGGC 
TTCCTTGGGG TGCAGAGGAA ATTTTGGCCA GTGACCTTGT CAATCCTTGG AGAGAGTAAA 
AAGGTTCTGC CAACCACAAA AGATGCTTGT TTTGCCTCAG CTGTAGAATG TCTACAGCAG 
ATCAGCACAA CATTTACCCC ATCAGACAAA CTTAAGGTCA TCCAGCAGAC TTTTGAAGAG 
ATCTCTCAGA GTGTCCTGGC ATCACTTCAG GAAGACTTCC TGTGGTCCAT GGATGACTTG 
TTTCCTGTTT TCTTATATGT GGTGCTACGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA 
CACCTCATTG AAGATCTAAT GGATCCATAT TTACAGCATG GGGAACAGGG TATAATGTTC 
ACCACCTTAA AGGCATGTTA CTACCAGATC CAGCGTGAGA AGCTTAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ALS2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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