MCF2L cDNA ORF clone, Gallus gallus(chicken)

The following MCF2L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCF2L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa142265 XM_025150076.1
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OGa30668 XM_015277842.2
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa30669 XM_015277843.2
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa30670 XM_015277844.1
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa30671 XM_015277845.2
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa30672 XM_015277846.1
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa30673 XM_015277847.2
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa30674 XM_015277848.2
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa30675 XM_015277849.2
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa30676 XM_015277850.2
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa30677 XM_015277853.2
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa30677 XM_015277852.1
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa30677 XM_025150075.1
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa30678 XM_015277854.2
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa25957 NM_001252063.1
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OGa30677 XM_015277853.1 Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa30676 XM_015277850.1 Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa30668 XM_015277842.1 Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa30669 XM_015277843.1 Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa30674 XM_015277848.1 Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa30675 XM_015277849.1 Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa30671 XM_015277845.1 Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa30673 XM_015277847.1 Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa30678 XM_015277854.1 Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa142265
    Clone ID Related Accession (Same CDS sequence) XM_025150076.1
    Accession Version XM_025150076.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGATGCT GGCTGCACGC TGAAGAAATG GCCCTAGAAG AATTGATGCA AAGATTAAAT 
    GCGGTTTCCC AGTGCACTGA TGAAATCATG CATCAGGATA TCATCCCCCT CTATGCAGCA
    GATATTCATG ACCAGTTAAA GAAACAATTT GCTTATCTGT CAGGTGGAAG AGGGGGAGAT
    GGATGCCCTG TTATCACTTT TCCCGACTAC CCAGCTTTCA GCGAGATCCC TGAGAAGGAG
    TTCCAGAATG TCCTGACCTA CCTGACCAGC ATCCCGAGTT TACGAGATGC TGGAATCGGG
    TTTATTCTTG TCATAGACCG CAGACAGGAC AAATGGACTT CTGTGAAAGC TTCCATACTG
    CGGATAGCAG CATCTTTCCC AGGGAACTTG CAACTCGTCC TCGTTCTGCG CCCAACAGGA
    CTTATTCATC GAGCTCTCTC AGACATTGCT TTCAAGTTCA ACAGAGATGA GTTTAAAATG
    AAAGTACCTA TCATAATGCT GGGCTCAGTA TCAGAACTGC AGGGTTACAT TGATAAAACA
    CAATTAACAG AGGATCTTGG TGGCACCCTG GATTACTGCC ATAACAGATG GCTATCCCTT
    CGCACTGCTA TAGAAGGTTT TGCCCAAAAG GTTAAGCAAA CAGCTCAGAT TCTTCAGTCC
    TTTGGGACTG AACTGGCTGA AACAGAACTA CCAAATGATG TGCAGTCAAC CAGCTCCCTT
    CTTGCAATAC ATACGGAAAA GAAGGACAAA ATGAAGGAAG ATATAAGGTT AGCAGTAGAA
    CAGGGAGACG ATATCCTTGG AAGTATTACA GAGCCAGTTA CTGAGAACCC TGAATACAAG
    CTGAATCAAG ACCAACTAGA CAATCAAACT ACAGTAGAAA GGCTTTTGGC TCAGTTACAT
    GAAACAGAGT CCGCCTTTGA TGAGTTTTGG ATTAAGCACC AGCAAAAACT TGAGCAGTGT
    CTGCACCTTC GGAATTTTGA ACAGAATTTT AGAGAGGTTA AAGCAGCTTT GGATATTGTG
    TCCGAGAGAC TGTCTGCTTT CACAGATGTG GGAAACAGCT GCTCACATGT GGAGCACATT
    TTGAAAGATC TTGCCAACTT TGAAGAAAAA TCATGTGAAA CTGTAGCAAA AGCCAGAATG
    CTAGCTTCAG AGGGTGATGC TTTTATTCAG AGCAATCATT ATGCTGTGGA TTCCATTATT
    CCAAAATGCA GTGAGCTTCA TCATCTATGT GATGCCTTCA CTACTGAGAT GGAACAGAGG
    AGGAATGTTC TTAATAAATC TCTAGAACTG CATGGCTTAC TGGAAAAGTC TATGAAGTGG
    TGCGATGAAG GAATTTATCT TCTGGCTTCA CAACCTGTAG ATAAGTGTCA GTCTCAGGAT
    GGTGCAGAAT CAGCATTACA GGAGATTGAG AAATTCCTAG ACACTGGTGC AGGCAATAAA
    ATTAAGGAGT TGAATAAAGT TTACAGAGAG TATGCACACA TCCTCAATGA GGACCTAAAG
    GAGCATATAC AAAAGGTTTT CCAGAAGCAA GAAAGCATGG AAGAAATGTT TCAAAAGAGA
    CAAGTAAGTC TTAAGAAACT GGCAGCTAAG CAAACACGTC CTGTTCAGCC AGTTGCACCT
    AGACCCGAAG CCTTTGTAAA ATCTCCTTGT ACCTCTCCAG GTCTTCAAAG AGATTGTGTA
    TCCAACACAG AAAGCGGTGT GCTTCGGAAA GTAAACTACA GAAGAGGAAA GAGTGAAATG
    AGTGAACTCC ATCAAAGCAG AGGAGGATCT ACAATGGATG ATGAAGAAAA CCTAGCTGTG
    TTACGGCAGC ATGTAATGAA TGAACTTATT GAAACGGAAC GAGCATATGT GGAAGAACTT
    CTCTGTGTTC TTGAGGGGTA TGCTGCAGAA ATGGACAACC CCTTAATGGC TCACCTCATT
    TCACCAGAAC TCCAAAACAA GAAGGATATT TTGTTTGGGA ATATGGAAGA AATTTATCAC
    TTTCACAACA GGATATTCTT GAGAGAACTA GAAAATTATG TAGAATACCC AGAACTGGTA
    GGACGGTGCT TTCTGGATCA GATGGAAGAT TTCCAGATTT ATGAGAAATA CTGTCAAAAC
    AAACCTCGAT CTGAAAGTTT GTGGAGGCAA TTTTCAGATT CCATATTCTT TCAGGAATGT
    CAAAGGAAAC TCGATCACAA GCTCAGTTTG GACTCATACC TGCTGAAGCC AGTTCAAAGA
    ATAACCAAAT ACCAATTATT GCTAAAGGAG ATGCTGAAGT ACAGTAAGAA CTGTGAAGGT
    GCTGAAGATC TTCAGGAGGC ATTAAATTCT ATATTGGGTA TTCTCAAAGC AGTCAATGAT
    TCTATGCACC AGATAGCCAT CACAGGCTAT GATGGAAACC TGAATGAGCT GGGCAAGCTC
    CTAATGCAGG GATCCTTCAA CGTCTGGACT GATCATAAAA AGGGTCACTC AAAGGTGAAA
    GATTTGGCAC GCTTCAAGCC AATGCAGCGA CACCTGTTCC TCCACGAGAA AGCAGTGCTG
    TTCTGTAAAA AGCGAGAAGA AAATGGAGAA GGATATGAGA AGGCACCTTC TTATAGCTAT
    AAACACTCCT TAAATATGGC AGCAGTTGGA ATAACAGAAA ATGTCAAAGG TGATGCAAAA
    AAATTTGAAA TCTGGTATAA TGCAAGGGAA GAAGTTTACA TCATACAGGC ACCAACTCCT
    GAAGTTAAAG CCACTTGGGT AAATGAAATA AGAAAAGTGC TGACTAGTCA ACTGCAGGCA
    TGCAGAGAAG CCAGTCAACA TAGGACACTT GAACACACTC AGAGTTTACC TCTACCAGCA
    TCATCCTGTA CAAGTCCTTC AAGGAACAAC ATCAGAAACG TGAAAAAAAT GGATGAGAGA
    AAAGCTGATC TCACAAGTCT AGAAGGTTAT GTCACCACAG TAGCACCAAA GTACCCCGAG
    AAAGGCAAAG GTTGGGCTAA AACGTCTCAT TCTCTTGATG CATCTGAAGA AAATGATGGC
    TGGTCCAGTG CTGAGGAGCC GCTGAATTCA TCGGATGCAG AAGAAGAAGG AGGAGGAGGA
    GGAGAGAGAA ATGAGATGAA GCTGACTCCT GGTAAATATA CAGTTGTGTC TGACTACGAG
    AAAGGAGTTT CTGAAGAATT TGCAGTGAAA GGTGGAGATC TAGTTCAACT GATTCGTGAA
    GGGGAGGAGG GACTTTGGTA TGTGAAGAAT CTGACCACTG GTAGAGAAGG TTGGATCCCA
    GCAAACAATC TGCTGATGCT CATAGGCAAT TCAAAATCAG CTCAATCTTT GAGCAGCTCT
    GAATCAGGCA CTGCTTCCAG CACTTTGAGC ACGTCATCAA GTTGCAGTGA TAGCTGCAAT
    GCCAGCAGCA CCAGCTTCTC AGACATTAAG GGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005844.1
    CDS2494..5889
    Translation

    Target ORF information:

    RefSeq Version XM_025150076.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025150076.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGATGCT GGCTGCACGC TGAAGAAATG GCCCTAGAAG AATTGATGCA AAGATTAAAT 
    GCGGTTTCCC AGTGCACTGA TGAAATCATG CATCAGGATA TCATCCCCCT CTATGCAGCA
    GATATTCATG ACCAGTTAAA GAAACAATTT GCTTATCTGT CAGGTGGAAG AGGGGGAGAT
    GGATGCCCTG TTATCACTTT TCCCGACTAC CCAGCTTTCA GCGAGATCCC TGAGAAGGAG
    TTCCAGAATG TCCTGACCTA CCTGACCAGC ATCCCGAGTT TACGAGATGC TGGAATCGGG
    TTTATTCTTG TCATAGACCG CAGACAGGAC AAATGGACTT CTGTGAAAGC TTCCATACTG
    CGGATAGCAG CATCTTTCCC AGGGAACTTG CAACTCGTCC TCGTTCTGCG CCCAACAGGA
    CTTATTCATC GAGCTCTCTC AGACATTGCT TTCAAGTTCA ACAGAGATGA GTTTAAAATG
    AAAGTACCTA TCATAATGCT GGGCTCAGTA TCAGAACTGC AGGGTTACAT TGATAAAACA
    CAATTAACAG AGGATCTTGG TGGCACCCTG GATTACTGCC ATAACAGATG GCTATCCCTT
    CGCACTGCTA TAGAAGGTTT TGCCCAAAAG GTTAAGCAAA CAGCTCAGAT TCTTCAGTCC
    TTTGGGACTG AACTGGCTGA AACAGAACTA CCAAATGATG TGCAGTCAAC CAGCTCCCTT
    CTTGCAATAC ATACGGAAAA GAAGGACAAA ATGAAGGAAG ATATAAGGTT AGCAGTAGAA
    CAGGGAGACG ATATCCTTGG AAGTATTACA GAGCCAGTTA CTGAGAACCC TGAATACAAG
    CTGAATCAAG ACCAACTAGA CAATCAAACT ACAGTAGAAA GGCTTTTGGC TCAGTTACAT
    GAAACAGAGT CCGCCTTTGA TGAGTTTTGG ATTAAGCACC AGCAAAAACT TGAGCAGTGT
    CTGCACCTTC GGAATTTTGA ACAGAATTTT AGAGAGGTTA AAGCAGCTTT GGATATTGTG
    TCCGAGAGAC TGTCTGCTTT CACAGATGTG GGAAACAGCT GCTCACATGT GGAGCACATT
    TTGAAAGATC TTGCCAACTT TGAAGAAAAA TCATGTGAAA CTGTAGCAAA AGCCAGAATG
    CTAGCTTCAG AGGGTGATGC TTTTATTCAG AGCAATCATT ATGCTGTGGA TTCCATTATT
    CCAAAATGCA GTGAGCTTCA TCATCTATGT GATGCCTTCA CTACTGAGAT GGAACAGAGG
    AGGAATGTTC TTAATAAATC TCTAGAACTG CATGGCTTAC TGGAAAAGTC TATGAAGTGG
    TGCGATGAAG GAATTTATCT TCTGGCTTCA CAACCTGTAG ATAAGTGTCA GTCTCAGGAT
    GGTGCAGAAT CAGCATTACA GGAGATTGAG AAATTCCTAG ACACTGGTGC AGGCAATAAA
    ATTAAGGAGT TGAATAAAGT TTACAGAGAG TATGCACACA TCCTCAATGA GGACCTAAAG
    GAGCATATAC AAAAGGTTTT CCAGAAGCAA GAAAGCATGG AAGAAATGTT TCAAAAGAGA
    CAAGTAAGTC TTAAGAAACT GGCAGCTAAG CAAACACGTC CTGTTCAGCC AGTTGCACCT
    AGACCCGAAG CCTTTGTAAA ATCTCCTTGT ACCTCTCCAG GTCTTCAAAG AGATTGTGTA
    TCCAACACAG AAAGCGGTGT GCTTCGGAAA GTAAACTACA GAAGAGGAAA GAGTGAAATG
    AGTGAACTCC ATCAAAGCAG AGGAGGATCT ACAATGGATG ATGAAGAAAA CCTAGCTGTG
    TTACGGCAGC ATGTAATGAA TGAACTTATT GAAACGGAAC GAGCATATGT GGAAGAACTT
    CTCTGTGTTC TTGAGGGGTA TGCTGCAGAA ATGGACAACC CCTTAATGGC TCACCTCATT
    TCACCAGAAC TCCAAAACAA GAAGGATATT TTGTTTGGGA ATATGGAAGA AATTTATCAC
    TTTCACAACA GGATATTCTT GAGAGAACTA GAAAATTATG TAGAATACCC AGAACTGGTA
    GGACGGTGCT TTCTGGATCA GATGGAAGAT TTCCAGATTT ATGAGAAATA CTGTCAAAAC
    AAACCTCGAT CTGAAAGTTT GTGGAGGCAA TTTTCAGATT CCATATTCTT TCAGGAATGT
    CAAAGGAAAC TCGATCACAA GCTCAGTTTG GACTCATACC TGCTGAAGCC AGTTCAAAGA
    ATAACCAAAT ACCAATTATT GCTAAAGGAG ATGCTGAAGT ACAGTAAGAA CTGTGAAGGT
    GCTGAAGATC TTCAGGAGGC ATTAAATTCT ATATTGGGTA TTCTCAAAGC AGTCAATGAT
    TCTATGCACC AGATAGCCAT CACAGGCTAT GATGGAAACC TGAATGAGCT GGGCAAGCTC
    CTAATGCAGG GATCCTTCAA CGTCTGGACT GATCATAAAA AGGGTCACTC AAAGGTGAAA
    GATTTGGCAC GCTTCAAGCC AATGCAGCGA CACCTGTTCC TCCACGAGAA AGCAGTGCTG
    TTCTGTAAAA AGCGAGAAGA AAATGGAGAA GGATATGAGA AGGCACCTTC TTATAGCTAT
    AAACACTCCT TAAATATGGC AGCAGTTGGA ATAACAGAAA ATGTCAAAGG TGATGCAAAA
    AAATTTGAAA TCTGGTATAA TGCAAGGGAA GAAGTTTACA TCATACAGGC ACCAACTCCT
    GAAGTTAAAG CCACTTGGGT AAATGAAATA AGAAAAGTGC TGACTAGTCA ACTGCAGGCA
    TGCAGAGAAG CCAGTCAACA TAGGACACTT GAACACACTC AGAGTTTACC TCTACCAGCA
    TCATCCTGTA CAAGTCCTTC AAGGAACAAC ATCAGAAACG TGAAAAAAAT GGATGAGAGA
    AAAGCTGATC TCACAAGTCT AGAAGGTTAT GTCACCACAG TAGCACCAAA GTACCCCGAG
    AAAGGCAAAG GTTGGGCTAA AACGTCTCAT TCTCTTGATG CATCTGAAGA AAATGATGGC
    TGGTCCAGTG CTGAGGAGCC GCTGAATTCA TCGGATGCAG AAGAAGAAGG AGGAGGAGGA
    GGAGAGAGAA ATGAGATGAA GCTGACTCCT GGTAAATATA CAGTTGTGTC TGACTACGAG
    AAAGGAGTTT CTGAAGAATT TGCAGTGAAA GGTGGAGATC TAGTTCAACT GATTCGTGAA
    GGGGAGGAGG GACTTTGGTA TGTGAAGAAT CTGACCACTG GTAGAGAAGG TTGGATCCCA
    GCAAACAATC TGCTGATGCT CATAGGCAAT TCAAAATCAG CTCAATCTTT GAGCAGCTCT
    GAATCAGGCA CTGCTTCCAG CACTTTGAGC ACGTCATCAA GTTGCAGTGA TAGCTGCAAT
    GCCAGCAGCA CCAGCTTCTC AGACATTAAG GGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30668
    Clone ID Related Accession (Same CDS sequence) XM_015277842.2 , XM_015277842.1
    Accession Version XM_015277842.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277842.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCTGACT ATTGGTACTG GAGGCTGGTC CCGTGCAGAG AAACCGGCAG CTGTAACTGT 
    TTTGCAACAG CCAACCAATC TAATGAGGAG AAAGAAGAAG ACACGAACCA CCAGGTTGTG
    GTGTCAGACA TCATGCAGCT GGTGCGAGAC AAGCAGGAGG TGCCGGAAAG AACAGCAGAT
    GAAATCATGC ATCAGGATAT CATCCCCCTC TATGCAGCAG ATATTCATGA CCAGTTAAAG
    AAACAATTTG CTTATCTGTC AGGTGGAAGA GGGGGAGATG GATGCCCTGT TATCACTTTT
    CCCGACTACC CAGCTTTCAG CGAGATCCCT GAGAAGGAGT TCCAGAATGT CCTGACCTAC
    CTGACCAGCA TCCCGAGTTT ACGAGATGCT GGAATCGGGT TTATTCTTGT CATAGACCGC
    AGACAGGACA AATGGACTTC TGTGAAAGCT TCCATACTGC GGATAGCAGC ATCTTTCCCA
    GGGAACTTGC AACTCGTCCT CGTTCTGCGC CCAACAGGAC TTATTCATCG AGCTCTCTCA
    GACATTGCTT TCAAGTTCAA CAGAGATGAG TTTAAAATGA AAGTACCTAT CATAATGCTG
    GGCTCAGTAT CAGAACTGCA GGGTTACATT GATAAAACAC AATTAACAGA GGATCTTGGT
    GGCACCCTGG ATTACTGCCA TAACAGATGG CTATCCCTTC GCACTGCTAT AGAAGGTTTT
    GCCCAAAAGG TTAAGCAAAC AGCTCAGATT CTTCAGTCCT TTGGGACTGA ACTGGCTGAA
    ACAGAACTAC CAAATGATGT GCAGTCAACC AGCTCCCTTC TTGCAATACA TACGGAAAAG
    AAGGACAAAA TGAAGGAAGA TATAAGGTTA GCAGTAGAAC AGGGAGACGA TATCCTTGGA
    AGTATTACAG AGCCAGTTAC TGAGAACCCT GAATACAAGC TGAATCAAGA CCAACTAGAC
    AATCAAACTA CAGTAGAAAG GCTTTTGGCT CAGTTACATG AAACAGAGTC CGCCTTTGAT
    GAGTTTTGGA TTAAGCACCA GCAAAAACTT GAGCAGTGTC TGCACCTTCG GAATTTTGAA
    CAGAATTTTA GAGAGGTTAA AGCAGCTTTG GATATTGTGT CCGAGAGACT GTCTGCTTTC
    ACAGATGTGG GAAACAGCTG CTCACATGTG GAGCACATTT TGAAAGATCT TGCCAACTTT
    GAAGAAAAAT CATGTGAAAC TGTAGCAAAA GCCAGAATGC TAGCTTCAGA GGGTGATGCT
    TTTATTCAGA GCAATCATTA TGCTGTGGAT TCCATTATTC CAAAATGCAG TGAGCTTCAT
    CATCTATGTG ATGCCTTCAC TACTGAGATG GAACAGAGGA GGAATGTTCT TAATAAATCT
    CTAGAACTGC ATGGCTTACT GGAAAAGTCT ATGAAGTGGT GCGATGAAGG AATTTATCTT
    CTGGCTTCAC AACCTGTAGA TAAGTGTCAG TCTCAGGATG GTGCAGAATC AGCATTACAG
    GAGATTGAGA AATTCCTAGA CACTGGTGCA GGCAATAAAA TTAAGGAGTT GAATAAAGTT
    TACAGAGAGT ATGCACACAT CCTCAATGAG GACCTAAAGG AGCATATACA AAAGGTTTTC
    CAGAAGCAAG AAAGCATGGA AGAAATGTTT CAAAAGAGAC AAGTAAGTCT TAAGAAACTG
    GCAGCTAAGC AAACACGTCC TGTTCAGCCA GTTGCACCTA GACCCGAAGC CTTTGTAAAA
    TCTCCTTGTA CCTCTCCAGG TCTTCAAAGA GATTGTGTAT CCAACACAGA AAGCGGTGTG
    CTTCGGAAAG TAAACTACAG AAGAGGAAAG AGTGAAATGA GTGAACTCCA TCAAAGCAGA
    GGAGGATCTA CAATGGATGA TGAAGAAAAC CTAGCTGTGT TACGGCAGCA TGTAATGAAT
    GAACTTATTG AAACGGAACG AGCATATGTG GAAGAACTTC TCTGTGTTCT TGAGGGGTAT
    GCTGCAGAAA TGGACAACCC CTTAATGGCT CACCTCATTT CACCAGAACT CCAAAACAAG
    AAGGATATTT TGTTTGGGAA TATGGAAGAA ATTTATCACT TTCACAACAG GATATTCTTG
    AGAGAACTAG AAAATTATGT AGAATACCCA GAACTGGTAG GACGGTGCTT TCTGGATCAG
    ATGGAAGATT TCCAGATTTA TGAGAAATAC TGTCAAAACA AACCTCGATC TGAAAGTTTG
    TGGAGGCAAT TTTCAGATTC CATATTCTTT CAGGAATGTC AAAGGAAACT CGATCACAAG
    CTCAGTTTGG ACTCATACCT GCTGAAGCCA GTTCAAAGAA TAACCAAATA CCAATTATTG
    CTAAAGGAGA TGCTGAAGTA CAGTAAGAAC TGTGAAGGTG CTGAAGATCT TCAGGAGGCA
    TTAAATTCTA TATTGGGTAT TCTCAAAGCA GTCAATGATT CTATGCACCA GATAGCCATC
    ACAGGCTATG ATGGAAACCT GAATGAGCTG GGCAAGCTCC TAATGCAGGG ATCCTTCAAC
    GTCTGGACTG ATCATAAAAA GGGTCACTCA AAGGTGAAAG ATTTGGCACG CTTCAAGCCA
    ATGCAGCGAC ACCTGTTCCT CCACGAGAAA GCAGTGCTGT TCTGTAAAAA GCGAGAAGAA
    AATGGAGAAG GATATGAGAA GGCACCTTCT TATAGCTATA AACACTCCTT AAATATGGCA
    GCAGTTGGAA TAACAGAAAA TGTCAAAGGT GATGCAAAAA AATTTGAAAT CTGGTATAAT
    GCAAGGGAAG AAGTTTACAT CATACAGGCA CCAACTCCTG AAGTTAAAGC CACTTGGGTA
    AATGAAATAA GAAAAGTGCT GACTAGTCAA CTGCAGGCAT GCAGAGAAGC CAGTCAACAT
    AGGACACTTG AACACACTCA GAGTTTACCT CTACCAGCAT CATCCTGTAC AAGTCCTTCA
    AGGAACAACA TCAGAAACGT GAAAAAAATG GATGAGAGAA AAGCTGATCT CACAAGTCTA
    GAAGGTTATG TCACCACAGT AGCACCAAAG TACCCCGAGA AAGGCAAAGA TGACACTAAC
    TCTACCTCAG AATGCTCTGT ACTGTCAAAA AAGCGCTTTA CGCTGCAGGG CTTTACTAAC
    CTCAAAAGTC AGAAAGCTTC TCCTACCAGT CCTGACAAAA AAGCTAAACG GCACGAAGTA
    GTGAGTGATC CAACTCCTTT TGGTGTAAGA GGTTGGGCTA AAACGTCTCA TTCTCTTGAT
    GCATCTGAAG AAAATGATGG CTGGTCCAGT GCTGAGGAGC CGCTGAATTC ATCGGATGCA
    GAAGAAGAAG GAGGAGGAGG AGGAGAGAGA AATGAGATGA AGCTGACTCC TGGTAAATAT
    ACAGTTGTGT CTGACTACGA GAAAGGAGTT TCTGAAGAAT TTGCAGTGAA AGGTGGAGAT
    CTAGTTCAAC TGATTCGTGA AGGGGAGGAG GGACTTTGGT ATGTGAAGAA TCTGACCACT
    GGTAGAGAAG GTTGGATCCC AGCAAACAAT CTGCTGATGC TCATAGGCAA TTCAAAATCA
    GCTCAATCTT TGAGCAGCTC TGAATCAGGC ACTGCTTCCA GCACTTTGAG CACGTCATCA
    AGTTGCAGTG ATAGCTGCAA TGCCAGCAGC ACCAGCTTCT CAGACATTAA GGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133328.1
    CDS307..3963
    Translation

    Target ORF information:

    RefSeq Version XM_015277842.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277842.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCTGACT ATTGGTACTG GAGGCTGGTC CCGTGCAGAG AAACCGGCAG CTGTAACTGT 
    TTTGCAACAG CCAACCAATC TAATGAGGAG AAAGAAGAAG ACACGAACCA CCAGGTTGTG
    GTGTCAGACA TCATGCAGCT GGTGCGAGAC AAGCAGGAGG TGCCGGAAAG AACAGCAGAT
    GAAATCATGC ATCAGGATAT CATCCCCCTC TATGCAGCAG ATATTCATGA CCAGTTAAAG
    AAACAATTTG CTTATCTGTC AGGTGGAAGA GGGGGAGATG GATGCCCTGT TATCACTTTT
    CCCGACTACC CAGCTTTCAG CGAGATCCCT GAGAAGGAGT TCCAGAATGT CCTGACCTAC
    CTGACCAGCA TCCCGAGTTT ACGAGATGCT GGAATCGGGT TTATTCTTGT CATAGACCGC
    AGACAGGACA AATGGACTTC TGTGAAAGCT TCCATACTGC GGATAGCAGC ATCTTTCCCA
    GGGAACTTGC AACTCGTCCT CGTTCTGCGC CCAACAGGAC TTATTCATCG AGCTCTCTCA
    GACATTGCTT TCAAGTTCAA CAGAGATGAG TTTAAAATGA AAGTACCTAT CATAATGCTG
    GGCTCAGTAT CAGAACTGCA GGGTTACATT GATAAAACAC AATTAACAGA GGATCTTGGT
    GGCACCCTGG ATTACTGCCA TAACAGATGG CTATCCCTTC GCACTGCTAT AGAAGGTTTT
    GCCCAAAAGG TTAAGCAAAC AGCTCAGATT CTTCAGTCCT TTGGGACTGA ACTGGCTGAA
    ACAGAACTAC CAAATGATGT GCAGTCAACC AGCTCCCTTC TTGCAATACA TACGGAAAAG
    AAGGACAAAA TGAAGGAAGA TATAAGGTTA GCAGTAGAAC AGGGAGACGA TATCCTTGGA
    AGTATTACAG AGCCAGTTAC TGAGAACCCT GAATACAAGC TGAATCAAGA CCAACTAGAC
    AATCAAACTA CAGTAGAAAG GCTTTTGGCT CAGTTACATG AAACAGAGTC CGCCTTTGAT
    GAGTTTTGGA TTAAGCACCA GCAAAAACTT GAGCAGTGTC TGCACCTTCG GAATTTTGAA
    CAGAATTTTA GAGAGGTTAA AGCAGCTTTG GATATTGTGT CCGAGAGACT GTCTGCTTTC
    ACAGATGTGG GAAACAGCTG CTCACATGTG GAGCACATTT TGAAAGATCT TGCCAACTTT
    GAAGAAAAAT CATGTGAAAC TGTAGCAAAA GCCAGAATGC TAGCTTCAGA GGGTGATGCT
    TTTATTCAGA GCAATCATTA TGCTGTGGAT TCCATTATTC CAAAATGCAG TGAGCTTCAT
    CATCTATGTG ATGCCTTCAC TACTGAGATG GAACAGAGGA GGAATGTTCT TAATAAATCT
    CTAGAACTGC ATGGCTTACT GGAAAAGTCT ATGAAGTGGT GCGATGAAGG AATTTATCTT
    CTGGCTTCAC AACCTGTAGA TAAGTGTCAG TCTCAGGATG GTGCAGAATC AGCATTACAG
    GAGATTGAGA AATTCCTAGA CACTGGTGCA GGCAATAAAA TTAAGGAGTT GAATAAAGTT
    TACAGAGAGT ATGCACACAT CCTCAATGAG GACCTAAAGG AGCATATACA AAAGGTTTTC
    CAGAAGCAAG AAAGCATGGA AGAAATGTTT CAAAAGAGAC AAGTAAGTCT TAAGAAACTG
    GCAGCTAAGC AAACACGTCC TGTTCAGCCA GTTGCACCTA GACCCGAAGC CTTTGTAAAA
    TCTCCTTGTA CCTCTCCAGG TCTTCAAAGA GATTGTGTAT CCAACACAGA AAGCGGTGTG
    CTTCGGAAAG TAAACTACAG AAGAGGAAAG AGTGAAATGA GTGAACTCCA TCAAAGCAGA
    GGAGGATCTA CAATGGATGA TGAAGAAAAC CTAGCTGTGT TACGGCAGCA TGTAATGAAT
    GAACTTATTG AAACGGAACG AGCATATGTG GAAGAACTTC TCTGTGTTCT TGAGGGGTAT
    GCTGCAGAAA TGGACAACCC CTTAATGGCT CACCTCATTT CACCAGAACT CCAAAACAAG
    AAGGATATTT TGTTTGGGAA TATGGAAGAA ATTTATCACT TTCACAACAG GATATTCTTG
    AGAGAACTAG AAAATTATGT AGAATACCCA GAACTGGTAG GACGGTGCTT TCTGGATCAG
    ATGGAAGATT TCCAGATTTA TGAGAAATAC TGTCAAAACA AACCTCGATC TGAAAGTTTG
    TGGAGGCAAT TTTCAGATTC CATATTCTTT CAGGAATGTC AAAGGAAACT CGATCACAAG
    CTCAGTTTGG ACTCATACCT GCTGAAGCCA GTTCAAAGAA TAACCAAATA CCAATTATTG
    CTAAAGGAGA TGCTGAAGTA CAGTAAGAAC TGTGAAGGTG CTGAAGATCT TCAGGAGGCA
    TTAAATTCTA TATTGGGTAT TCTCAAAGCA GTCAATGATT CTATGCACCA GATAGCCATC
    ACAGGCTATG ATGGAAACCT GAATGAGCTG GGCAAGCTCC TAATGCAGGG ATCCTTCAAC
    GTCTGGACTG ATCATAAAAA GGGTCACTCA AAGGTGAAAG ATTTGGCACG CTTCAAGCCA
    ATGCAGCGAC ACCTGTTCCT CCACGAGAAA GCAGTGCTGT TCTGTAAAAA GCGAGAAGAA
    AATGGAGAAG GATATGAGAA GGCACCTTCT TATAGCTATA AACACTCCTT AAATATGGCA
    GCAGTTGGAA TAACAGAAAA TGTCAAAGGT GATGCAAAAA AATTTGAAAT CTGGTATAAT
    GCAAGGGAAG AAGTTTACAT CATACAGGCA CCAACTCCTG AAGTTAAAGC CACTTGGGTA
    AATGAAATAA GAAAAGTGCT GACTAGTCAA CTGCAGGCAT GCAGAGAAGC CAGTCAACAT
    AGGACACTTG AACACACTCA GAGTTTACCT CTACCAGCAT CATCCTGTAC AAGTCCTTCA
    AGGAACAACA TCAGAAACGT GAAAAAAATG GATGAGAGAA AAGCTGATCT CACAAGTCTA
    GAAGGTTATG TCACCACAGT AGCACCAAAG TACCCCGAGA AAGGCAAAGA TGACACTAAC
    TCTACCTCAG AATGCTCTGT ACTGTCAAAA AAGCGCTTTA CGCTGCAGGG CTTTACTAAC
    CTCAAAAGTC AGAAAGCTTC TCCTACCAGT CCTGACAAAA AAGCTAAACG GCACGAAGTA
    GTGAGTGATC CAACTCCTTT TGGTGTAAGA GGTTGGGCTA AAACGTCTCA TTCTCTTGAT
    GCATCTGAAG AAAATGATGG CTGGTCCAGT GCTGAGGAGC CGCTGAATTC ATCGGATGCA
    GAAGAAGAAG GAGGAGGAGG AGGAGAGAGA AATGAGATGA AGCTGACTCC TGGTAAATAT
    ACAGTTGTGT CTGACTACGA GAAAGGAGTT TCTGAAGAAT TTGCAGTGAA AGGTGGAGAT
    CTAGTTCAAC TGATTCGTGA AGGGGAGGAG GGACTTTGGT ATGTGAAGAA TCTGACCACT
    GGTAGAGAAG GTTGGATCCC AGCAAACAAT CTGCTGATGC TCATAGGCAA TTCAAAATCA
    GCTCAATCTT TGAGCAGCTC TGAATCAGGC ACTGCTTCCA GCACTTTGAG CACGTCATCA
    AGTTGCAGTG ATAGCTGCAA TGCCAGCAGC ACCAGCTTCT CAGACATTAA GGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30668
    Clone ID Related Accession (Same CDS sequence) XM_015277842.2 , XM_015277842.1
    Accession Version XM_015277842.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCTGACT ATTGGTACTG GAGGCTGGTC CCGTGCAGAG AAACCGGCAG CTGTAACTGT 
    TTTGCAACAG CCAACCAATC TAATGAGGAG AAAGAAGAAG ACACGAACCA CCAGGTTGTG
    GTGTCAGACA TCATGCAGCT GGTGCGAGAC AAGCAGGAGG TGCCGGAAAG AACAGCAGAT
    GAAATCATGC ATCAGGATAT CATCCCCCTC TATGCAGCAG ATATTCATGA CCAGTTAAAG
    AAACAATTTG CTTATCTGTC AGGTGGAAGA GGGGGAGATG GATGCCCTGT TATCACTTTT
    CCCGACTACC CAGCTTTCAG CGAGATCCCT GAGAAGGAGT TCCAGAATGT CCTGACCTAC
    CTGACCAGCA TCCCGAGTTT ACGAGATGCT GGAATCGGGT TTATTCTTGT CATAGACCGC
    AGACAGGACA AATGGACTTC TGTGAAAGCT TCCATACTGC GGATAGCAGC ATCTTTCCCA
    GGGAACTTGC AACTCGTCCT CGTTCTGCGC CCAACAGGAC TTATTCATCG AGCTCTCTCA
    GACATTGCTT TCAAGTTCAA CAGAGATGAG TTTAAAATGA AAGTACCTAT CATAATGCTG
    GGCTCAGTAT CAGAACTGCA GGGTTACATT GATAAAACAC AATTAACAGA GGATCTTGGT
    GGCACCCTGG ATTACTGCCA TAACAGATGG CTATCCCTTC GCACTGCTAT AGAAGGTTTT
    GCCCAAAAGG TTAAGCAAAC AGCTCAGATT CTTCAGTCCT TTGGGACTGA ACTGGCTGAA
    ACAGAACTAC CAAATGATGT GCAGTCAACC AGCTCCCTTC TTGCAATACA TACGGAAAAG
    AAGGACAAAA TGAAGGAAGA TATAAGGTTA GCAGTAGAAC AGGGAGACGA TATCCTTGGA
    AGTATTACAG AGCCAGTTAC TGAGAACCCT GAATACAAGC TGAATCAAGA CCAACTAGAC
    AATCAAACTA CAGTAGAAAG GCTTTTGGCT CAGTTACATG AAACAGAGTC CGCCTTTGAT
    GAGTTTTGGA TTAAGCACCA GCAAAAACTT GAGCAGTGTC TGCACCTTCG GAATTTTGAA
    CAGAATTTTA GAGAGGTTAA AGCAGCTTTG GATATTGTGT CCGAGAGACT GTCTGCTTTC
    ACAGATGTGG GAAACAGCTG CTCACATGTG GAGCACATTT TGAAAGATCT TGCCAACTTT
    GAAGAAAAAT CATGTGAAAC TGTAGCAAAA GCCAGAATGC TAGCTTCAGA GGGTGATGCT
    TTTATTCAGA GCAATCATTA TGCTGTGGAT TCCATTATTC CAAAATGCAG TGAGCTTCAT
    CATCTATGTG ATGCCTTCAC TACTGAGATG GAACAGAGGA GGAATGTTCT TAATAAATCT
    CTAGAACTGC ATGGCTTACT GGAAAAGTCT ATGAAGTGGT GCGATGAAGG AATTTATCTT
    CTGGCTTCAC AACCTGTAGA TAAGTGTCAG TCTCAGGATG GTGCAGAATC AGCATTACAG
    GAGATTGAGA AATTCCTAGA CACTGGTGCA GGCAATAAAA TTAAGGAGTT GAATAAAGTT
    TACAGAGAGT ATGCACACAT CCTCAATGAG GACCTAAAGG AGCATATACA AAAGGTTTTC
    CAGAAGCAAG AAAGCATGGA AGAAATGTTT CAAAAGAGAC AAGTAAGTCT TAAGAAACTG
    GCAGCTAAGC AAACACGTCC TGTTCAGCCA GTTGCACCTA GACCCGAAGC CTTTGTAAAA
    TCTCCTTGTA CCTCTCCAGG TCTTCAAAGA GATTGTGTAT CCAACACAGA AAGCGGTGTG
    CTTCGGAAAG TAAACTACAG AAGAGGAAAG AGTGAAATGA GTGAACTCCA TCAAAGCAGA
    GGAGGATCTA CAATGGATGA TGAAGAAAAC CTAGCTGTGT TACGGCAGCA TGTAATGAAT
    GAACTTATTG AAACGGAACG AGCATATGTG GAAGAACTTC TCTGTGTTCT TGAGGGGTAT
    GCTGCAGAAA TGGACAACCC CTTAATGGCT CACCTCATTT CACCAGAACT CCAAAACAAG
    AAGGATATTT TGTTTGGGAA TATGGAAGAA ATTTATCACT TTCACAACAG GATATTCTTG
    AGAGAACTAG AAAATTATGT AGAATACCCA GAACTGGTAG GACGGTGCTT TCTGGATCAG
    ATGGAAGATT TCCAGATTTA TGAGAAATAC TGTCAAAACA AACCTCGATC TGAAAGTTTG
    TGGAGGCAAT TTTCAGATTC CATATTCTTT CAGGAATGTC AAAGGAAACT CGATCACAAG
    CTCAGTTTGG ACTCATACCT GCTGAAGCCA GTTCAAAGAA TAACCAAATA CCAATTATTG
    CTAAAGGAGA TGCTGAAGTA CAGTAAGAAC TGTGAAGGTG CTGAAGATCT TCAGGAGGCA
    TTAAATTCTA TATTGGGTAT TCTCAAAGCA GTCAATGATT CTATGCACCA GATAGCCATC
    ACAGGCTATG ATGGAAACCT GAATGAGCTG GGCAAGCTCC TAATGCAGGG ATCCTTCAAC
    GTCTGGACTG ATCATAAAAA GGGTCACTCA AAGGTGAAAG ATTTGGCACG CTTCAAGCCA
    ATGCAGCGAC ACCTGTTCCT CCACGAGAAA GCAGTGCTGT TCTGTAAAAA GCGAGAAGAA
    AATGGAGAAG GATATGAGAA GGCACCTTCT TATAGCTATA AACACTCCTT AAATATGGCA
    GCAGTTGGAA TAACAGAAAA TGTCAAAGGT GATGCAAAAA AATTTGAAAT CTGGTATAAT
    GCAAGGGAAG AAGTTTACAT CATACAGGCA CCAACTCCTG AAGTTAAAGC CACTTGGGTA
    AATGAAATAA GAAAAGTGCT GACTAGTCAA CTGCAGGCAT GCAGAGAAGC CAGTCAACAT
    AGGACACTTG AACACACTCA GAGTTTACCT CTACCAGCAT CATCCTGTAC AAGTCCTTCA
    AGGAACAACA TCAGAAACGT GAAAAAAATG GATGAGAGAA AAGCTGATCT CACAAGTCTA
    GAAGGTTATG TCACCACAGT AGCACCAAAG TACCCCGAGA AAGGCAAAGA TGACACTAAC
    TCTACCTCAG AATGCTCTGT ACTGTCAAAA AAGCGCTTTA CGCTGCAGGG CTTTACTAAC
    CTCAAAAGTC AGAAAGCTTC TCCTACCAGT CCTGACAAAA AAGCTAAACG GCACGAAGTA
    GTGAGTGATC CAACTCCTTT TGGTGTAAGA GGTTGGGCTA AAACGTCTCA TTCTCTTGAT
    GCATCTGAAG AAAATGATGG CTGGTCCAGT GCTGAGGAGC CGCTGAATTC ATCGGATGCA
    GAAGAAGAAG GAGGAGGAGG AGGAGAGAGA AATGAGATGA AGCTGACTCC TGGTAAATAT
    ACAGTTGTGT CTGACTACGA GAAAGGAGTT TCTGAAGAAT TTGCAGTGAA AGGTGGAGAT
    CTAGTTCAAC TGATTCGTGA AGGGGAGGAG GGACTTTGGT ATGTGAAGAA TCTGACCACT
    GGTAGAGAAG GTTGGATCCC AGCAAACAAT CTGCTGATGC TCATAGGCAA TTCAAAATCA
    GCTCAATCTT TGAGCAGCTC TGAATCAGGC ACTGCTTCCA GCACTTTGAG CACGTCATCA
    AGTTGCAGTG ATAGCTGCAA TGCCAGCAGC ACCAGCTTCT CAGACATTAA GGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133328.1
    CDS116..3772
    Translation

    Target ORF information:

    RefSeq Version XM_015277842.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277842.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCTGACT ATTGGTACTG GAGGCTGGTC CCGTGCAGAG AAACCGGCAG CTGTAACTGT 
    TTTGCAACAG CCAACCAATC TAATGAGGAG AAAGAAGAAG ACACGAACCA CCAGGTTGTG
    GTGTCAGACA TCATGCAGCT GGTGCGAGAC AAGCAGGAGG TGCCGGAAAG AACAGCAGAT
    GAAATCATGC ATCAGGATAT CATCCCCCTC TATGCAGCAG ATATTCATGA CCAGTTAAAG
    AAACAATTTG CTTATCTGTC AGGTGGAAGA GGGGGAGATG GATGCCCTGT TATCACTTTT
    CCCGACTACC CAGCTTTCAG CGAGATCCCT GAGAAGGAGT TCCAGAATGT CCTGACCTAC
    CTGACCAGCA TCCCGAGTTT ACGAGATGCT GGAATCGGGT TTATTCTTGT CATAGACCGC
    AGACAGGACA AATGGACTTC TGTGAAAGCT TCCATACTGC GGATAGCAGC ATCTTTCCCA
    GGGAACTTGC AACTCGTCCT CGTTCTGCGC CCAACAGGAC TTATTCATCG AGCTCTCTCA
    GACATTGCTT TCAAGTTCAA CAGAGATGAG TTTAAAATGA AAGTACCTAT CATAATGCTG
    GGCTCAGTAT CAGAACTGCA GGGTTACATT GATAAAACAC AATTAACAGA GGATCTTGGT
    GGCACCCTGG ATTACTGCCA TAACAGATGG CTATCCCTTC GCACTGCTAT AGAAGGTTTT
    GCCCAAAAGG TTAAGCAAAC AGCTCAGATT CTTCAGTCCT TTGGGACTGA ACTGGCTGAA
    ACAGAACTAC CAAATGATGT GCAGTCAACC AGCTCCCTTC TTGCAATACA TACGGAAAAG
    AAGGACAAAA TGAAGGAAGA TATAAGGTTA GCAGTAGAAC AGGGAGACGA TATCCTTGGA
    AGTATTACAG AGCCAGTTAC TGAGAACCCT GAATACAAGC TGAATCAAGA CCAACTAGAC
    AATCAAACTA CAGTAGAAAG GCTTTTGGCT CAGTTACATG AAACAGAGTC CGCCTTTGAT
    GAGTTTTGGA TTAAGCACCA GCAAAAACTT GAGCAGTGTC TGCACCTTCG GAATTTTGAA
    CAGAATTTTA GAGAGGTTAA AGCAGCTTTG GATATTGTGT CCGAGAGACT GTCTGCTTTC
    ACAGATGTGG GAAACAGCTG CTCACATGTG GAGCACATTT TGAAAGATCT TGCCAACTTT
    GAAGAAAAAT CATGTGAAAC TGTAGCAAAA GCCAGAATGC TAGCTTCAGA GGGTGATGCT
    TTTATTCAGA GCAATCATTA TGCTGTGGAT TCCATTATTC CAAAATGCAG TGAGCTTCAT
    CATCTATGTG ATGCCTTCAC TACTGAGATG GAACAGAGGA GGAATGTTCT TAATAAATCT
    CTAGAACTGC ATGGCTTACT GGAAAAGTCT ATGAAGTGGT GCGATGAAGG AATTTATCTT
    CTGGCTTCAC AACCTGTAGA TAAGTGTCAG TCTCAGGATG GTGCAGAATC AGCATTACAG
    GAGATTGAGA AATTCCTAGA CACTGGTGCA GGCAATAAAA TTAAGGAGTT GAATAAAGTT
    TACAGAGAGT ATGCACACAT CCTCAATGAG GACCTAAAGG AGCATATACA AAAGGTTTTC
    CAGAAGCAAG AAAGCATGGA AGAAATGTTT CAAAAGAGAC AAGTAAGTCT TAAGAAACTG
    GCAGCTAAGC AAACACGTCC TGTTCAGCCA GTTGCACCTA GACCCGAAGC CTTTGTAAAA
    TCTCCTTGTA CCTCTCCAGG TCTTCAAAGA GATTGTGTAT CCAACACAGA AAGCGGTGTG
    CTTCGGAAAG TAAACTACAG AAGAGGAAAG AGTGAAATGA GTGAACTCCA TCAAAGCAGA
    GGAGGATCTA CAATGGATGA TGAAGAAAAC CTAGCTGTGT TACGGCAGCA TGTAATGAAT
    GAACTTATTG AAACGGAACG AGCATATGTG GAAGAACTTC TCTGTGTTCT TGAGGGGTAT
    GCTGCAGAAA TGGACAACCC CTTAATGGCT CACCTCATTT CACCAGAACT CCAAAACAAG
    AAGGATATTT TGTTTGGGAA TATGGAAGAA ATTTATCACT TTCACAACAG GATATTCTTG
    AGAGAACTAG AAAATTATGT AGAATACCCA GAACTGGTAG GACGGTGCTT TCTGGATCAG
    ATGGAAGATT TCCAGATTTA TGAGAAATAC TGTCAAAACA AACCTCGATC TGAAAGTTTG
    TGGAGGCAAT TTTCAGATTC CATATTCTTT CAGGAATGTC AAAGGAAACT CGATCACAAG
    CTCAGTTTGG ACTCATACCT GCTGAAGCCA GTTCAAAGAA TAACCAAATA CCAATTATTG
    CTAAAGGAGA TGCTGAAGTA CAGTAAGAAC TGTGAAGGTG CTGAAGATCT TCAGGAGGCA
    TTAAATTCTA TATTGGGTAT TCTCAAAGCA GTCAATGATT CTATGCACCA GATAGCCATC
    ACAGGCTATG ATGGAAACCT GAATGAGCTG GGCAAGCTCC TAATGCAGGG ATCCTTCAAC
    GTCTGGACTG ATCATAAAAA GGGTCACTCA AAGGTGAAAG ATTTGGCACG CTTCAAGCCA
    ATGCAGCGAC ACCTGTTCCT CCACGAGAAA GCAGTGCTGT TCTGTAAAAA GCGAGAAGAA
    AATGGAGAAG GATATGAGAA GGCACCTTCT TATAGCTATA AACACTCCTT AAATATGGCA
    GCAGTTGGAA TAACAGAAAA TGTCAAAGGT GATGCAAAAA AATTTGAAAT CTGGTATAAT
    GCAAGGGAAG AAGTTTACAT CATACAGGCA CCAACTCCTG AAGTTAAAGC CACTTGGGTA
    AATGAAATAA GAAAAGTGCT GACTAGTCAA CTGCAGGCAT GCAGAGAAGC CAGTCAACAT
    AGGACACTTG AACACACTCA GAGTTTACCT CTACCAGCAT CATCCTGTAC AAGTCCTTCA
    AGGAACAACA TCAGAAACGT GAAAAAAATG GATGAGAGAA AAGCTGATCT CACAAGTCTA
    GAAGGTTATG TCACCACAGT AGCACCAAAG TACCCCGAGA AAGGCAAAGA TGACACTAAC
    TCTACCTCAG AATGCTCTGT ACTGTCAAAA AAGCGCTTTA CGCTGCAGGG CTTTACTAAC
    CTCAAAAGTC AGAAAGCTTC TCCTACCAGT CCTGACAAAA AAGCTAAACG GCACGAAGTA
    GTGAGTGATC CAACTCCTTT TGGTGTAAGA GGTTGGGCTA AAACGTCTCA TTCTCTTGAT
    GCATCTGAAG AAAATGATGG CTGGTCCAGT GCTGAGGAGC CGCTGAATTC ATCGGATGCA
    GAAGAAGAAG GAGGAGGAGG AGGAGAGAGA AATGAGATGA AGCTGACTCC TGGTAAATAT
    ACAGTTGTGT CTGACTACGA GAAAGGAGTT TCTGAAGAAT TTGCAGTGAA AGGTGGAGAT
    CTAGTTCAAC TGATTCGTGA AGGGGAGGAG GGACTTTGGT ATGTGAAGAA TCTGACCACT
    GGTAGAGAAG GTTGGATCCC AGCAAACAAT CTGCTGATGC TCATAGGCAA TTCAAAATCA
    GCTCAATCTT TGAGCAGCTC TGAATCAGGC ACTGCTTCCA GCACTTTGAG CACGTCATCA
    AGTTGCAGTG ATAGCTGCAA TGCCAGCAGC ACCAGCTTCT CAGACATTAA GGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30669
    Clone ID Related Accession (Same CDS sequence) XM_015277843.2 , XM_015277843.1
    Accession Version XM_015277843.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCTGACT ATTGGTACTG GAGGCTGGTC CCGTGCAGAG AAACCGGCAG CTGTAACTGT 
    TTTGCAACAG CCAACCAATC TAATGAGGAG AAAGAAGAAG ACACGAACCA CCAGGTTGTG
    GTGTCAGACA TCATGCAGCT GGTGCGAGAC AAGCAGGAGG TGCCGGAAAG AACAGCAGAT
    GAAATCATGC ATCAGGATAT CATCCCCCTC TATGCAGCAG ATATTCATGA CCAGTTAAAG
    AAACAATTTG CTTATCTGTC AGGTGGAAGA GGGGGAGATG GATGCCCTGT TATCACTTTT
    CCCGACTACC CAGCTTTCAG CGAGATCCCT GAGAAGGAGT TCCAGAATGT CCTGACCTAC
    CTGACCAGCA TCCCGAGTTT ACGAGATGCT GGAATCGGGT TTATTCTTGT CATAGACCGC
    AGACAGGACA AATGGACTTC TGTGAAAGCT TCCATACTGC GGATAGCAGC ATCTTTCCCA
    GGGAACTTGC AACTCGTCCT CGTTCTGCGC CCAACAGGAC TTATTCATCG AGCTCTCTCA
    GACATTGCTT TCAAGTTCAA CAGAGATGAG TTTAAAATGA AAGTACCTAT CATAATGCTG
    GGCTCAGTAT CAGAACTGCA GGGTTACATT GATAAAACAC AATTAACAGA GGATCTTGGT
    GGCACCCTGG ATTACTGCCA TAACAGATGG CTATCCCTTC GCACTGCTAT AGAAGGTTTT
    GCCCAAAAGG TTAAGCAAAC AGCTCAGATT CTTCAGTCCT TTGGGACTGA ACTGGCTGAA
    ACAGAACTAC CAAATGATGT GCAGTCAACC AGCTCCCTTC TTGCAATACA TACGGAAAAG
    AAGGACAAAA TGAAGGAAGA TATAAGGTTA GCAGTAGAAC AGGGAGACGA TATCCTTGGA
    AGTATTACAG AGCCAGTTAC TGAGAACCCT GAATACAAGC TGAATCAAGA CCAACTAGAC
    AATCAAACTA CAGTAGAAAG GCTTTTGGCT CAGTTACATG AAACAGAGTC CGCCTTTGAT
    GAGTTTTGGA TTAAGCACCA GCAAAAACTT GAGCAGTGTC TGCACCTTCG GAATTTTGAA
    CAGAATTTTA GAGAGGTTAA AGCAGCTTTG GATATTGTGT CCGAGAGACT GTCTGCTTTC
    ACAGATGTGG GAAACAGCTG CTCACATGTG GAGCACATTT TGAAAGATCT TGCCAACTTT
    GAAGAAAAAT CATGTGAAAC TGTAGCAAAA GCCAGAATGC TAGCTTCAGA GGGTGATGCT
    TTTATTCAGA GCAATCATTA TGCTGTGGAT TCCATTATTC CAAAATGCAG TGAGCTTCAT
    CATCTATGTG ATGCCTTCAC TACTGAGATG GAACAGAGGA GGAATGTTCT TAATAAATCT
    CTAGAACTGC ATGGCTTACT GGAAAAGTCT ATGAAGTGGT GCGATGAAGG AATTTATCTT
    CTGGCTTCAC AACCTGTAGA TAAGTGTCAG TCTCAGGATG GTGCAGAATC AGCATTACAG
    GAGATTGAGA AATTCCTAGA CACTGGTGCA GGCAATAAAA TTAAGGAGTT GAATAAAGTT
    TACAGAGAGT ATGCACACAT CCTCAATGAG GACCTAAAGG AGCATATACA AAAGGTTTTC
    CAGAAGCAAG AAAGCATGGA AGAAATGTTT CAAAAGAGAC AAGTAAGTCT TAAGAAACTG
    GCAGCTAAGC AAACACGTCC TGTTCAGCCA GTTGCACCTA GACCCGAAGC CTTTGTAAAA
    TCTCCTTGTA CCTCTCCAGG TCTTCAAAGA GATTGTGTAT CCAACACAGA AAGCGGTGTG
    CTTCGGAAAG TAAACTACAG AAGAGGAAAG AGTGAAATGA GTGAACTCCA TCAAAGCAGA
    GGAGGATCTA CAATGGATGA TGAAGAAAAC CTAGCTGTGT TACGGCAGCA TGTAATGAAT
    GAACTTATTG AAACGGAACG AGCATATGTG GAAGAACTTC TCTGTGTTCT TGAGGGGTAT
    GCTGCAGAAA TGGACAACCC CTTAATGGCT CACCTCATTT CACCAGAACT CCAAAACAAG
    AAGGATATTT TGTTTGGGAA TATGGAAGAA ATTTATCACT TTCACAACAG GATATTCTTG
    AGAGAACTAG AAAATTATGT AGAATACCCA GAACTGGTAG GACGGTGCTT TCTGGATCAG
    ATGGAAGATT TCCAGATTTA TGAGAAATAC TGTCAAAACA AACCTCGATC TGAAAGTTTG
    TGGAGGCAAT TTTCAGATTC CATATTCTTT CAGGAATGTC AAAGGAAACT CGATCACAAG
    CTCAGTTTGG ACTCATACCT GCTGAAGCCA GTTCAAAGAA TAACCAAATA CCAATTATTG
    CTAAAGGAGA TGCTGAAGTA CAGTAAGAAC TGTGAAGGTG CTGAAGATCT TCAGGAGGCA
    TTAAATTCTA TATTGGGTAT TCTCAAAGCA GTCAATGATT CTATGCACCA GATAGCCATC
    ACAGGCTATG ATGGAAACCT GAATGAGCTG GGCAAGCTCC TAATGCAGGG ATCCTTCAAC
    GTCTGGACTG ATCATAAAAA GGGTCACTCA AAGGTGAAAG ATTTGGCACG CTTCAAGCCA
    ATGCAGCGAC ACCTGTTCCT CCACGAGAAA GCAGTGCTGT TCTGTAAAAA GCGAGAAGAA
    AATGGAGAAG GATATGAGAA GGCACCTTCT TATAGCTATA AACACTCCTT AAATATGGCA
    GCAGTTGGAA TAACAGAAAA TGTCAAAGGT GATGCAAAAA AATTTGAAAT CTGGTATAAT
    GCAAGGGAAG AAGTTTACAT CATACAGGCA CCAACTCCTG AAGTTAAAGC CACTTGGGTA
    AATGAAATAA GAAAAGTGCT GACTAGTCAA CTGCAGGCAT GCAGAGAAGC CAGTCAACAT
    AGGACACTTG AACACACTCA GAGTTTACCT CTACCAGCAT CATCCTGTAC AAGTCCTTCA
    AGGAACAACA TCAGAAACGT GAAAAAAATG GATGAGAGAA AAGCTGATCT CACAAGTCTA
    GAAGGTTATG TCACCACAGT AGCACCAAAG TACCCCGAGA AAGGCAAAGA AACTCCATCT
    TCTACTGCTA AAAGCCAGAC ATTGCCAATG ATCCTTCTTA CAGGACCAGC TTCTCCTACC
    AGTCCTGACA AAAAAGCTAA ACGGCACGAA GTAGTGAGTG ATCCAACTCC TTTTGGTGTA
    AGAGGTTGGG CTAAAACGTC TCATTCTCTT GATGCATCTG AAGAAAATGA TGGCTGGTCC
    AGTGCTGAGG AGCCGCTGAA TTCATCGGAT GCAGAAGAAG AAGGAGGAGG AGGAGGAGAG
    AGAAATGAGA TGAAGCTGAC TCCTGGTAAA TATACAGTTG TGTCTGACTA CGAGAAAGGA
    GTTTCTGAAG AATTTGCAGT GAAAGGTGGA GATCTAGTTC AACTGATTCG TGAAGGGGAG
    GAGGGACTTT GGTATGTGAA GAATCTGACC ACTGGTAGAG AAGGTTGGAT CCCAGCAAAC
    AATCTGCTGA TGCTCATAGG CAATTCAAAA TCAGCTCAAT CTTTGAGCAG CTCTGAATCA
    GGCACTGCTT CCAGCACTTT GAGCACGTCA TCAAGTTGCA GTGATAGCTG CAATGCCAGC
    AGCACCAGCT TCTCAGACAT TAAGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133329.1
    CDS130..3759
    Translation

    Target ORF information:

    RefSeq Version XM_015277843.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277843.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCTGACT ATTGGTACTG GAGGCTGGTC CCGTGCAGAG AAACCGGCAG CTGTAACTGT 
    TTTGCAACAG CCAACCAATC TAATGAGGAG AAAGAAGAAG ACACGAACCA CCAGGTTGTG
    GTGTCAGACA TCATGCAGCT GGTGCGAGAC AAGCAGGAGG TGCCGGAAAG AACAGCAGAT
    GAAATCATGC ATCAGGATAT CATCCCCCTC TATGCAGCAG ATATTCATGA CCAGTTAAAG
    AAACAATTTG CTTATCTGTC AGGTGGAAGA GGGGGAGATG GATGCCCTGT TATCACTTTT
    CCCGACTACC CAGCTTTCAG CGAGATCCCT GAGAAGGAGT TCCAGAATGT CCTGACCTAC
    CTGACCAGCA TCCCGAGTTT ACGAGATGCT GGAATCGGGT TTATTCTTGT CATAGACCGC
    AGACAGGACA AATGGACTTC TGTGAAAGCT TCCATACTGC GGATAGCAGC ATCTTTCCCA
    GGGAACTTGC AACTCGTCCT CGTTCTGCGC CCAACAGGAC TTATTCATCG AGCTCTCTCA
    GACATTGCTT TCAAGTTCAA CAGAGATGAG TTTAAAATGA AAGTACCTAT CATAATGCTG
    GGCTCAGTAT CAGAACTGCA GGGTTACATT GATAAAACAC AATTAACAGA GGATCTTGGT
    GGCACCCTGG ATTACTGCCA TAACAGATGG CTATCCCTTC GCACTGCTAT AGAAGGTTTT
    GCCCAAAAGG TTAAGCAAAC AGCTCAGATT CTTCAGTCCT TTGGGACTGA ACTGGCTGAA
    ACAGAACTAC CAAATGATGT GCAGTCAACC AGCTCCCTTC TTGCAATACA TACGGAAAAG
    AAGGACAAAA TGAAGGAAGA TATAAGGTTA GCAGTAGAAC AGGGAGACGA TATCCTTGGA
    AGTATTACAG AGCCAGTTAC TGAGAACCCT GAATACAAGC TGAATCAAGA CCAACTAGAC
    AATCAAACTA CAGTAGAAAG GCTTTTGGCT CAGTTACATG AAACAGAGTC CGCCTTTGAT
    GAGTTTTGGA TTAAGCACCA GCAAAAACTT GAGCAGTGTC TGCACCTTCG GAATTTTGAA
    CAGAATTTTA GAGAGGTTAA AGCAGCTTTG GATATTGTGT CCGAGAGACT GTCTGCTTTC
    ACAGATGTGG GAAACAGCTG CTCACATGTG GAGCACATTT TGAAAGATCT TGCCAACTTT
    GAAGAAAAAT CATGTGAAAC TGTAGCAAAA GCCAGAATGC TAGCTTCAGA GGGTGATGCT
    TTTATTCAGA GCAATCATTA TGCTGTGGAT TCCATTATTC CAAAATGCAG TGAGCTTCAT
    CATCTATGTG ATGCCTTCAC TACTGAGATG GAACAGAGGA GGAATGTTCT TAATAAATCT
    CTAGAACTGC ATGGCTTACT GGAAAAGTCT ATGAAGTGGT GCGATGAAGG AATTTATCTT
    CTGGCTTCAC AACCTGTAGA TAAGTGTCAG TCTCAGGATG GTGCAGAATC AGCATTACAG
    GAGATTGAGA AATTCCTAGA CACTGGTGCA GGCAATAAAA TTAAGGAGTT GAATAAAGTT
    TACAGAGAGT ATGCACACAT CCTCAATGAG GACCTAAAGG AGCATATACA AAAGGTTTTC
    CAGAAGCAAG AAAGCATGGA AGAAATGTTT CAAAAGAGAC AAGTAAGTCT TAAGAAACTG
    GCAGCTAAGC AAACACGTCC TGTTCAGCCA GTTGCACCTA GACCCGAAGC CTTTGTAAAA
    TCTCCTTGTA CCTCTCCAGG TCTTCAAAGA GATTGTGTAT CCAACACAGA AAGCGGTGTG
    CTTCGGAAAG TAAACTACAG AAGAGGAAAG AGTGAAATGA GTGAACTCCA TCAAAGCAGA
    GGAGGATCTA CAATGGATGA TGAAGAAAAC CTAGCTGTGT TACGGCAGCA TGTAATGAAT
    GAACTTATTG AAACGGAACG AGCATATGTG GAAGAACTTC TCTGTGTTCT TGAGGGGTAT
    GCTGCAGAAA TGGACAACCC CTTAATGGCT CACCTCATTT CACCAGAACT CCAAAACAAG
    AAGGATATTT TGTTTGGGAA TATGGAAGAA ATTTATCACT TTCACAACAG GATATTCTTG
    AGAGAACTAG AAAATTATGT AGAATACCCA GAACTGGTAG GACGGTGCTT TCTGGATCAG
    ATGGAAGATT TCCAGATTTA TGAGAAATAC TGTCAAAACA AACCTCGATC TGAAAGTTTG
    TGGAGGCAAT TTTCAGATTC CATATTCTTT CAGGAATGTC AAAGGAAACT CGATCACAAG
    CTCAGTTTGG ACTCATACCT GCTGAAGCCA GTTCAAAGAA TAACCAAATA CCAATTATTG
    CTAAAGGAGA TGCTGAAGTA CAGTAAGAAC TGTGAAGGTG CTGAAGATCT TCAGGAGGCA
    TTAAATTCTA TATTGGGTAT TCTCAAAGCA GTCAATGATT CTATGCACCA GATAGCCATC
    ACAGGCTATG ATGGAAACCT GAATGAGCTG GGCAAGCTCC TAATGCAGGG ATCCTTCAAC
    GTCTGGACTG ATCATAAAAA GGGTCACTCA AAGGTGAAAG ATTTGGCACG CTTCAAGCCA
    ATGCAGCGAC ACCTGTTCCT CCACGAGAAA GCAGTGCTGT TCTGTAAAAA GCGAGAAGAA
    AATGGAGAAG GATATGAGAA GGCACCTTCT TATAGCTATA AACACTCCTT AAATATGGCA
    GCAGTTGGAA TAACAGAAAA TGTCAAAGGT GATGCAAAAA AATTTGAAAT CTGGTATAAT
    GCAAGGGAAG AAGTTTACAT CATACAGGCA CCAACTCCTG AAGTTAAAGC CACTTGGGTA
    AATGAAATAA GAAAAGTGCT GACTAGTCAA CTGCAGGCAT GCAGAGAAGC CAGTCAACAT
    AGGACACTTG AACACACTCA GAGTTTACCT CTACCAGCAT CATCCTGTAC AAGTCCTTCA
    AGGAACAACA TCAGAAACGT GAAAAAAATG GATGAGAGAA AAGCTGATCT CACAAGTCTA
    GAAGGTTATG TCACCACAGT AGCACCAAAG TACCCCGAGA AAGGCAAAGA AACTCCATCT
    TCTACTGCTA AAAGCCAGAC ATTGCCAATG ATCCTTCTTA CAGGACCAGC TTCTCCTACC
    AGTCCTGACA AAAAAGCTAA ACGGCACGAA GTAGTGAGTG ATCCAACTCC TTTTGGTGTA
    AGAGGTTGGG CTAAAACGTC TCATTCTCTT GATGCATCTG AAGAAAATGA TGGCTGGTCC
    AGTGCTGAGG AGCCGCTGAA TTCATCGGAT GCAGAAGAAG AAGGAGGAGG AGGAGGAGAG
    AGAAATGAGA TGAAGCTGAC TCCTGGTAAA TATACAGTTG TGTCTGACTA CGAGAAAGGA
    GTTTCTGAAG AATTTGCAGT GAAAGGTGGA GATCTAGTTC AACTGATTCG TGAAGGGGAG
    GAGGGACTTT GGTATGTGAA GAATCTGACC ACTGGTAGAG AAGGTTGGAT CCCAGCAAAC
    AATCTGCTGA TGCTCATAGG CAATTCAAAA TCAGCTCAAT CTTTGAGCAG CTCTGAATCA
    GGCACTGCTT CCAGCACTTT GAGCACGTCA TCAAGTTGCA GTGATAGCTG CAATGCCAGC
    AGCACCAGCT TCTCAGACAT TAAGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30669
    Clone ID Related Accession (Same CDS sequence) XM_015277843.2 , XM_015277843.1
    Accession Version XM_015277843.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCTGACT ATTGGTACTG GAGGCTGGTC CCGTGCAGAG AAACCGGCAG CTGTAACTGT 
    TTTGCAACAG CCAACCAATC TAATGAGGAG AAAGAAGAAG ACACGAACCA CCAGGTTGTG
    GTGTCAGACA TCATGCAGCT GGTGCGAGAC AAGCAGGAGG TGCCGGAAAG AACAGCAGAT
    GAAATCATGC ATCAGGATAT CATCCCCCTC TATGCAGCAG ATATTCATGA CCAGTTAAAG
    AAACAATTTG CTTATCTGTC AGGTGGAAGA GGGGGAGATG GATGCCCTGT TATCACTTTT
    CCCGACTACC CAGCTTTCAG CGAGATCCCT GAGAAGGAGT TCCAGAATGT CCTGACCTAC
    CTGACCAGCA TCCCGAGTTT ACGAGATGCT GGAATCGGGT TTATTCTTGT CATAGACCGC
    AGACAGGACA AATGGACTTC TGTGAAAGCT TCCATACTGC GGATAGCAGC ATCTTTCCCA
    GGGAACTTGC AACTCGTCCT CGTTCTGCGC CCAACAGGAC TTATTCATCG AGCTCTCTCA
    GACATTGCTT TCAAGTTCAA CAGAGATGAG TTTAAAATGA AAGTACCTAT CATAATGCTG
    GGCTCAGTAT CAGAACTGCA GGGTTACATT GATAAAACAC AATTAACAGA GGATCTTGGT
    GGCACCCTGG ATTACTGCCA TAACAGATGG CTATCCCTTC GCACTGCTAT AGAAGGTTTT
    GCCCAAAAGG TTAAGCAAAC AGCTCAGATT CTTCAGTCCT TTGGGACTGA ACTGGCTGAA
    ACAGAACTAC CAAATGATGT GCAGTCAACC AGCTCCCTTC TTGCAATACA TACGGAAAAG
    AAGGACAAAA TGAAGGAAGA TATAAGGTTA GCAGTAGAAC AGGGAGACGA TATCCTTGGA
    AGTATTACAG AGCCAGTTAC TGAGAACCCT GAATACAAGC TGAATCAAGA CCAACTAGAC
    AATCAAACTA CAGTAGAAAG GCTTTTGGCT CAGTTACATG AAACAGAGTC CGCCTTTGAT
    GAGTTTTGGA TTAAGCACCA GCAAAAACTT GAGCAGTGTC TGCACCTTCG GAATTTTGAA
    CAGAATTTTA GAGAGGTTAA AGCAGCTTTG GATATTGTGT CCGAGAGACT GTCTGCTTTC
    ACAGATGTGG GAAACAGCTG CTCACATGTG GAGCACATTT TGAAAGATCT TGCCAACTTT
    GAAGAAAAAT CATGTGAAAC TGTAGCAAAA GCCAGAATGC TAGCTTCAGA GGGTGATGCT
    TTTATTCAGA GCAATCATTA TGCTGTGGAT TCCATTATTC CAAAATGCAG TGAGCTTCAT
    CATCTATGTG ATGCCTTCAC TACTGAGATG GAACAGAGGA GGAATGTTCT TAATAAATCT
    CTAGAACTGC ATGGCTTACT GGAAAAGTCT ATGAAGTGGT GCGATGAAGG AATTTATCTT
    CTGGCTTCAC AACCTGTAGA TAAGTGTCAG TCTCAGGATG GTGCAGAATC AGCATTACAG
    GAGATTGAGA AATTCCTAGA CACTGGTGCA GGCAATAAAA TTAAGGAGTT GAATAAAGTT
    TACAGAGAGT ATGCACACAT CCTCAATGAG GACCTAAAGG AGCATATACA AAAGGTTTTC
    CAGAAGCAAG AAAGCATGGA AGAAATGTTT CAAAAGAGAC AAGTAAGTCT TAAGAAACTG
    GCAGCTAAGC AAACACGTCC TGTTCAGCCA GTTGCACCTA GACCCGAAGC CTTTGTAAAA
    TCTCCTTGTA CCTCTCCAGG TCTTCAAAGA GATTGTGTAT CCAACACAGA AAGCGGTGTG
    CTTCGGAAAG TAAACTACAG AAGAGGAAAG AGTGAAATGA GTGAACTCCA TCAAAGCAGA
    GGAGGATCTA CAATGGATGA TGAAGAAAAC CTAGCTGTGT TACGGCAGCA TGTAATGAAT
    GAACTTATTG AAACGGAACG AGCATATGTG GAAGAACTTC TCTGTGTTCT TGAGGGGTAT
    GCTGCAGAAA TGGACAACCC CTTAATGGCT CACCTCATTT CACCAGAACT CCAAAACAAG
    AAGGATATTT TGTTTGGGAA TATGGAAGAA ATTTATCACT TTCACAACAG GATATTCTTG
    AGAGAACTAG AAAATTATGT AGAATACCCA GAACTGGTAG GACGGTGCTT TCTGGATCAG
    ATGGAAGATT TCCAGATTTA TGAGAAATAC TGTCAAAACA AACCTCGATC TGAAAGTTTG
    TGGAGGCAAT TTTCAGATTC CATATTCTTT CAGGAATGTC AAAGGAAACT CGATCACAAG
    CTCAGTTTGG ACTCATACCT GCTGAAGCCA GTTCAAAGAA TAACCAAATA CCAATTATTG
    CTAAAGGAGA TGCTGAAGTA CAGTAAGAAC TGTGAAGGTG CTGAAGATCT TCAGGAGGCA
    TTAAATTCTA TATTGGGTAT TCTCAAAGCA GTCAATGATT CTATGCACCA GATAGCCATC
    ACAGGCTATG ATGGAAACCT GAATGAGCTG GGCAAGCTCC TAATGCAGGG ATCCTTCAAC
    GTCTGGACTG ATCATAAAAA GGGTCACTCA AAGGTGAAAG ATTTGGCACG CTTCAAGCCA
    ATGCAGCGAC ACCTGTTCCT CCACGAGAAA GCAGTGCTGT TCTGTAAAAA GCGAGAAGAA
    AATGGAGAAG GATATGAGAA GGCACCTTCT TATAGCTATA AACACTCCTT AAATATGGCA
    GCAGTTGGAA TAACAGAAAA TGTCAAAGGT GATGCAAAAA AATTTGAAAT CTGGTATAAT
    GCAAGGGAAG AAGTTTACAT CATACAGGCA CCAACTCCTG AAGTTAAAGC CACTTGGGTA
    AATGAAATAA GAAAAGTGCT GACTAGTCAA CTGCAGGCAT GCAGAGAAGC CAGTCAACAT
    AGGACACTTG AACACACTCA GAGTTTACCT CTACCAGCAT CATCCTGTAC AAGTCCTTCA
    AGGAACAACA TCAGAAACGT GAAAAAAATG GATGAGAGAA AAGCTGATCT CACAAGTCTA
    GAAGGTTATG TCACCACAGT AGCACCAAAG TACCCCGAGA AAGGCAAAGA AACTCCATCT
    TCTACTGCTA AAAGCCAGAC ATTGCCAATG ATCCTTCTTA CAGGACCAGC TTCTCCTACC
    AGTCCTGACA AAAAAGCTAA ACGGCACGAA GTAGTGAGTG ATCCAACTCC TTTTGGTGTA
    AGAGGTTGGG CTAAAACGTC TCATTCTCTT GATGCATCTG AAGAAAATGA TGGCTGGTCC
    AGTGCTGAGG AGCCGCTGAA TTCATCGGAT GCAGAAGAAG AAGGAGGAGG AGGAGGAGAG
    AGAAATGAGA TGAAGCTGAC TCCTGGTAAA TATACAGTTG TGTCTGACTA CGAGAAAGGA
    GTTTCTGAAG AATTTGCAGT GAAAGGTGGA GATCTAGTTC AACTGATTCG TGAAGGGGAG
    GAGGGACTTT GGTATGTGAA GAATCTGACC ACTGGTAGAG AAGGTTGGAT CCCAGCAAAC
    AATCTGCTGA TGCTCATAGG CAATTCAAAA TCAGCTCAAT CTTTGAGCAG CTCTGAATCA
    GGCACTGCTT CCAGCACTTT GAGCACGTCA TCAAGTTGCA GTGATAGCTG CAATGCCAGC
    AGCACCAGCT TCTCAGACAT TAAGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133329.1
    CDS116..3745
    Translation

    Target ORF information:

    RefSeq Version XM_015277843.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277843.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCTGACT ATTGGTACTG GAGGCTGGTC CCGTGCAGAG AAACCGGCAG CTGTAACTGT 
    TTTGCAACAG CCAACCAATC TAATGAGGAG AAAGAAGAAG ACACGAACCA CCAGGTTGTG
    GTGTCAGACA TCATGCAGCT GGTGCGAGAC AAGCAGGAGG TGCCGGAAAG AACAGCAGAT
    GAAATCATGC ATCAGGATAT CATCCCCCTC TATGCAGCAG ATATTCATGA CCAGTTAAAG
    AAACAATTTG CTTATCTGTC AGGTGGAAGA GGGGGAGATG GATGCCCTGT TATCACTTTT
    CCCGACTACC CAGCTTTCAG CGAGATCCCT GAGAAGGAGT TCCAGAATGT CCTGACCTAC
    CTGACCAGCA TCCCGAGTTT ACGAGATGCT GGAATCGGGT TTATTCTTGT CATAGACCGC
    AGACAGGACA AATGGACTTC TGTGAAAGCT TCCATACTGC GGATAGCAGC ATCTTTCCCA
    GGGAACTTGC AACTCGTCCT CGTTCTGCGC CCAACAGGAC TTATTCATCG AGCTCTCTCA
    GACATTGCTT TCAAGTTCAA CAGAGATGAG TTTAAAATGA AAGTACCTAT CATAATGCTG
    GGCTCAGTAT CAGAACTGCA GGGTTACATT GATAAAACAC AATTAACAGA GGATCTTGGT
    GGCACCCTGG ATTACTGCCA TAACAGATGG CTATCCCTTC GCACTGCTAT AGAAGGTTTT
    GCCCAAAAGG TTAAGCAAAC AGCTCAGATT CTTCAGTCCT TTGGGACTGA ACTGGCTGAA
    ACAGAACTAC CAAATGATGT GCAGTCAACC AGCTCCCTTC TTGCAATACA TACGGAAAAG
    AAGGACAAAA TGAAGGAAGA TATAAGGTTA GCAGTAGAAC AGGGAGACGA TATCCTTGGA
    AGTATTACAG AGCCAGTTAC TGAGAACCCT GAATACAAGC TGAATCAAGA CCAACTAGAC
    AATCAAACTA CAGTAGAAAG GCTTTTGGCT CAGTTACATG AAACAGAGTC CGCCTTTGAT
    GAGTTTTGGA TTAAGCACCA GCAAAAACTT GAGCAGTGTC TGCACCTTCG GAATTTTGAA
    CAGAATTTTA GAGAGGTTAA AGCAGCTTTG GATATTGTGT CCGAGAGACT GTCTGCTTTC
    ACAGATGTGG GAAACAGCTG CTCACATGTG GAGCACATTT TGAAAGATCT TGCCAACTTT
    GAAGAAAAAT CATGTGAAAC TGTAGCAAAA GCCAGAATGC TAGCTTCAGA GGGTGATGCT
    TTTATTCAGA GCAATCATTA TGCTGTGGAT TCCATTATTC CAAAATGCAG TGAGCTTCAT
    CATCTATGTG ATGCCTTCAC TACTGAGATG GAACAGAGGA GGAATGTTCT TAATAAATCT
    CTAGAACTGC ATGGCTTACT GGAAAAGTCT ATGAAGTGGT GCGATGAAGG AATTTATCTT
    CTGGCTTCAC AACCTGTAGA TAAGTGTCAG TCTCAGGATG GTGCAGAATC AGCATTACAG
    GAGATTGAGA AATTCCTAGA CACTGGTGCA GGCAATAAAA TTAAGGAGTT GAATAAAGTT
    TACAGAGAGT ATGCACACAT CCTCAATGAG GACCTAAAGG AGCATATACA AAAGGTTTTC
    CAGAAGCAAG AAAGCATGGA AGAAATGTTT CAAAAGAGAC AAGTAAGTCT TAAGAAACTG
    GCAGCTAAGC AAACACGTCC TGTTCAGCCA GTTGCACCTA GACCCGAAGC CTTTGTAAAA
    TCTCCTTGTA CCTCTCCAGG TCTTCAAAGA GATTGTGTAT CCAACACAGA AAGCGGTGTG
    CTTCGGAAAG TAAACTACAG AAGAGGAAAG AGTGAAATGA GTGAACTCCA TCAAAGCAGA
    GGAGGATCTA CAATGGATGA TGAAGAAAAC CTAGCTGTGT TACGGCAGCA TGTAATGAAT
    GAACTTATTG AAACGGAACG AGCATATGTG GAAGAACTTC TCTGTGTTCT TGAGGGGTAT
    GCTGCAGAAA TGGACAACCC CTTAATGGCT CACCTCATTT CACCAGAACT CCAAAACAAG
    AAGGATATTT TGTTTGGGAA TATGGAAGAA ATTTATCACT TTCACAACAG GATATTCTTG
    AGAGAACTAG AAAATTATGT AGAATACCCA GAACTGGTAG GACGGTGCTT TCTGGATCAG
    ATGGAAGATT TCCAGATTTA TGAGAAATAC TGTCAAAACA AACCTCGATC TGAAAGTTTG
    TGGAGGCAAT TTTCAGATTC CATATTCTTT CAGGAATGTC AAAGGAAACT CGATCACAAG
    CTCAGTTTGG ACTCATACCT GCTGAAGCCA GTTCAAAGAA TAACCAAATA CCAATTATTG
    CTAAAGGAGA TGCTGAAGTA CAGTAAGAAC TGTGAAGGTG CTGAAGATCT TCAGGAGGCA
    TTAAATTCTA TATTGGGTAT TCTCAAAGCA GTCAATGATT CTATGCACCA GATAGCCATC
    ACAGGCTATG ATGGAAACCT GAATGAGCTG GGCAAGCTCC TAATGCAGGG ATCCTTCAAC
    GTCTGGACTG ATCATAAAAA GGGTCACTCA AAGGTGAAAG ATTTGGCACG CTTCAAGCCA
    ATGCAGCGAC ACCTGTTCCT CCACGAGAAA GCAGTGCTGT TCTGTAAAAA GCGAGAAGAA
    AATGGAGAAG GATATGAGAA GGCACCTTCT TATAGCTATA AACACTCCTT AAATATGGCA
    GCAGTTGGAA TAACAGAAAA TGTCAAAGGT GATGCAAAAA AATTTGAAAT CTGGTATAAT
    GCAAGGGAAG AAGTTTACAT CATACAGGCA CCAACTCCTG AAGTTAAAGC CACTTGGGTA
    AATGAAATAA GAAAAGTGCT GACTAGTCAA CTGCAGGCAT GCAGAGAAGC CAGTCAACAT
    AGGACACTTG AACACACTCA GAGTTTACCT CTACCAGCAT CATCCTGTAC AAGTCCTTCA
    AGGAACAACA TCAGAAACGT GAAAAAAATG GATGAGAGAA AAGCTGATCT CACAAGTCTA
    GAAGGTTATG TCACCACAGT AGCACCAAAG TACCCCGAGA AAGGCAAAGA AACTCCATCT
    TCTACTGCTA AAAGCCAGAC ATTGCCAATG ATCCTTCTTA CAGGACCAGC TTCTCCTACC
    AGTCCTGACA AAAAAGCTAA ACGGCACGAA GTAGTGAGTG ATCCAACTCC TTTTGGTGTA
    AGAGGTTGGG CTAAAACGTC TCATTCTCTT GATGCATCTG AAGAAAATGA TGGCTGGTCC
    AGTGCTGAGG AGCCGCTGAA TTCATCGGAT GCAGAAGAAG AAGGAGGAGG AGGAGGAGAG
    AGAAATGAGA TGAAGCTGAC TCCTGGTAAA TATACAGTTG TGTCTGACTA CGAGAAAGGA
    GTTTCTGAAG AATTTGCAGT GAAAGGTGGA GATCTAGTTC AACTGATTCG TGAAGGGGAG
    GAGGGACTTT GGTATGTGAA GAATCTGACC ACTGGTAGAG AAGGTTGGAT CCCAGCAAAC
    AATCTGCTGA TGCTCATAGG CAATTCAAAA TCAGCTCAAT CTTTGAGCAG CTCTGAATCA
    GGCACTGCTT CCAGCACTTT GAGCACGTCA TCAAGTTGCA GTGATAGCTG CAATGCCAGC
    AGCACCAGCT TCTCAGACAT TAAGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30670
    Clone ID Related Accession (Same CDS sequence) XM_015277844.1
    Accession Version XM_015277844.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCGCAAC GTCGGCTTTT AAAGGATGGA CATGAAAGTC ACTTTAGGAT CCTACCAAGA 
    TTCAGCCAAA TCCTTCACAG TAGAAGGCAC TCGCGAAATC ACACGCGCCA AATATGCTCT
    GATGAAATCA TGCATCAGGA TATCATCCCC CTCTATGCAG CAGATATTCA TGACCAGTTA
    AAGAAACAAT TTGCTTATCT GTCAGGTGGA AGAGGGGGAG ATGGATGCCC TGTTATCACT
    TTTCCCGACT ACCCAGCTTT CAGCGAGATC CCTGAGAAGG AGTTCCAGAA TGTCCTGACC
    TACCTGACCA GCATCCCGAG TTTACGAGAT GCTGGAATCG GGTTTATTCT TGTCATAGAC
    CGCAGACAGG ACAAATGGAC TTCTGTGAAA GCTTCCATAC TGCGGATAGC AGCATCTTTC
    CCAGGGAACT TGCAACTCGT CCTCGTTCTG CGCCCAACAG GACTTATTCA TCGAGCTCTC
    TCAGACATTG CTTTCAAGTT CAACAGAGAT GAGTTTAAAA TGAAAGTACC TATCATAATG
    CTGGGCTCAG TATCAGAACT GCAGGGTTAC ATTGATAAAA CACAATTAAC AGAGGATCTT
    GGTGGCACCC TGGATTACTG CCATAACAGA TGGCTATCCC TTCGCACTGC TATAGAAGGT
    TTTGCCCAAA AGGTTAAGCA AACAGCTCAG ATTCTTCAGT CCTTTGGGAC TGAACTGGCT
    GAAACAGAAC TACCAAATGA TGTGCAGTCA ACCAGCTCCC TTCTTGCAAT ACATACGGAA
    AAGAAGGACA AAATGAAGGA AGATATAAGG TTAGCAGTAG AACAGGGAGA CGATATCCTT
    GGAAGTATTA CAGAGCCAGT TACTGAGAAC CCTGAATACA AGCTGAATCA AGACCAACTA
    GACAATCAAA CTACAGTAGA AAGGCTTTTG GCTCAGTTAC ATGAAACAGA GTCCGCCTTT
    GATGAGTTTT GGATTAAGCA CCAGCAAAAA CTTGAGCAGT GTCTGCACCT TCGGAATTTT
    GAACAGAATT TTAGAGAGGT TAAAGCAGCT TTGGATATTG TGTCCGAGAG ACTGTCTGCT
    TTCACAGATG TGGGAAACAG CTGCTCACAT GTGGAGCACA TTTTGAAAGA TCTTGCCAAC
    TTTGAAGAAA AATCATGTGA AACTGTAGCA AAAGCCAGAA TGCTAGCTTC AGAGGGTGAT
    GCTTTTATTC AGAGCAATCA TTATGCTGTG GATTCCATTA TTCCAAAATG CAGTGAGCTT
    CATCATCTAT GTGATGCCTT CACTACTGAG ATGGAACAGA GGAGGAATGT TCTTAATAAA
    TCTCTAGAAC TGCATGGCTT ACTGGAAAAG TCTATGAAGT GGTGCGATGA AGGAATTTAT
    CTTCTGGCTT CACAACCTGT AGATAAGTGT CAGTCTCAGG ATGGTGCAGA ATCAGCATTA
    CAGGAGATTG AGAAATTCCT AGACACTGGT GCAGGCAATA AAATTAAGGA GTTGAATAAA
    GTTTACAGAG AGTATGCACA CATCCTCAAT GAGGACCTAA AGGAGCATAT ACAAAAGGTT
    TTCCAGAAGC AAGAAAGCAT GGAAGAAATG TTTCAAAAGA GACAAGTAAG TCTTAAGAAA
    CTGGCAGCTA AGCAAACACG TCCTGTTCAG CCAGTTGCAC CTAGACCCGA AGCCTTTGTA
    AAATCTCCTT GTACCTCTCC AGGTCTTCAA AGAGATTGTG TATCCAACAC AGAAAGCGGT
    GTGCTTCGGA AAGTAAACTA CAGAAGAGGA AAGAGTGAAA TGAGTGAACT CCATCAAAGC
    AGAGGAGGAT CTACAATGGA TGATGAAGAA AACCTAGCTG TGTTACGGCA GCATGTAATG
    AATGAACTTA TTGAAACGGA ACGAGCATAT GTGGAAGAAC TTCTCTGTGT TCTTGAGGGG
    TATGCTGCAG AAATGGACAA CCCCTTAATG GCTCACCTCA TTTCACCAGA ACTCCAAAAC
    AAGAAGGATA TTTTGTTTGG GAATATGGAA GAAATTTATC ACTTTCACAA CAGGATATTC
    TTGAGAGAAC TAGAAAATTA TGTAGAATAC CCAGAACTGG TAGGACGGTG CTTTCTGGAT
    CAGATGGAAG ATTTCCAGAT TTATGAGAAA TACTGTCAAA ACAAACCTCG ATCTGAAAGT
    TTGTGGAGGC AATTTTCAGA TTCCATATTC TTTCAGGAAT GTCAAAGGAA ACTCGATCAC
    AAGCTCAGTT TGGACTCATA CCTGCTGAAG CCAGTTCAAA GAATAACCAA ATACCAATTA
    TTGCTAAAGG AGATGCTGAA GTACAGTAAG AACTGTGAAG GTGCTGAAGA TCTTCAGGAG
    GCATTAAATT CTATATTGGG TATTCTCAAA GCAGTCAATG ATTCTATGCA CCAGATAGCC
    ATCACAGGCT ATGATGGAAA CCTGAATGAG CTGGGCAAGC TCCTAATGCA GGGATCCTTC
    AACGTCTGGA CTGATCATAA AAAGGGTCAC TCAAAGGTGA AAGATTTGGC ACGCTTCAAG
    CCAATGCAGC GACACCTGTT CCTCCACGAG AAAGCAGTGC TGTTCTGTAA AAAGCGAGAA
    GAAAATGGAG AAGGATATGA GAAGGCACCT TCTTATAGCT ATAAACACTC CTTAAATATG
    GCAGCAGTTG GAATAACAGA AAATGTCAAA GGTGATGCAA AAAAATTTGA AATCTGGTAT
    AATGCAAGGG AAGAAGTTTA CATCATACAG GCACCAACTC CTGAAGTTAA AGCCACTTGG
    GTAAATGAAA TAAGAAAAGT GCTGACTAGT CAACTGCAGG CATGCAGAGA AGCCAGTCAA
    CATAGGACAC TTGAACACAC TCAGAGTTTA CCTCTACCAG CATCATCCTG TACAAGTCCT
    TCAAGGAACA ACATCAGAAA CGTGAAAAAA ATGGATGAGA GAAAAGCTGA TCTCACAAGT
    CTAGAAGGTT ATGTCACCAC AGTAGCACCA AAGTACCCCG AGAAAGGCAA AGATGACACT
    AACTCTACCT CAGAATGCTC TGTACTGTCA AAAAAGCGCT TTACGCTGCA GGGCTTTACT
    AACCTCAAAA GTCAGAAAGC TTCTCCTACC AGTCCTGACA AAAAAGCTAA ACGGCACGAA
    GTAGTGAGTG ATCCAACTCC TTTTGGTGTA AGAGGTTGGG CTAAAACGTC TCATTCTCTT
    GATGCATCTG AAGAAAATGA TGGCTGGTCC AGTGCTGAGG AGCCGCTGAA TTCATCGGAT
    GCAGAAGAAG AAGGAGGAGG AGGAGGAGAG AGAAATGAGA TGAAGCTGAC TCCTGGTAAA
    TATACAGTTG TGTCTGACTA CGAGAAAGGA GTTTCTGAAG AATTTGCAGT GAAAGGTGGA
    GATCTAGTTC AACTGATTCG TGAAGGGGAG GAGGGACTTT GGTATGTGAA GAATCTGACC
    ACTGGTAGAG AAGGTTGGAT CCCAGCAAAC AATCTGCTGA TGCTCATAGG CAATTCAAAA
    TCAGCTCAAT CTTTGAGCAG CTCTGAATCA GGCACTGCTT CCAGCACTTT GAGCACGTCA
    TCAAGTTGCA GTGATAGCTG CAATGCCAGC AGCACCAGCT TCTCAGACAT TAAGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133330.1
    CDS1128..4727
    Translation

    Target ORF information:

    RefSeq Version XM_015277844.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277844.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCGCAAC GTCGGCTTTT AAAGGATGGA CATGAAAGTC ACTTTAGGAT CCTACCAAGA 
    TTCAGCCAAA TCCTTCACAG TAGAAGGCAC TCGCGAAATC ACACGCGCCA AATATGCTCT
    GATGAAATCA TGCATCAGGA TATCATCCCC CTCTATGCAG CAGATATTCA TGACCAGTTA
    AAGAAACAAT TTGCTTATCT GTCAGGTGGA AGAGGGGGAG ATGGATGCCC TGTTATCACT
    TTTCCCGACT ACCCAGCTTT CAGCGAGATC CCTGAGAAGG AGTTCCAGAA TGTCCTGACC
    TACCTGACCA GCATCCCGAG TTTACGAGAT GCTGGAATCG GGTTTATTCT TGTCATAGAC
    CGCAGACAGG ACAAATGGAC TTCTGTGAAA GCTTCCATAC TGCGGATAGC AGCATCTTTC
    CCAGGGAACT TGCAACTCGT CCTCGTTCTG CGCCCAACAG GACTTATTCA TCGAGCTCTC
    TCAGACATTG CTTTCAAGTT CAACAGAGAT GAGTTTAAAA TGAAAGTACC TATCATAATG
    CTGGGCTCAG TATCAGAACT GCAGGGTTAC ATTGATAAAA CACAATTAAC AGAGGATCTT
    GGTGGCACCC TGGATTACTG CCATAACAGA TGGCTATCCC TTCGCACTGC TATAGAAGGT
    TTTGCCCAAA AGGTTAAGCA AACAGCTCAG ATTCTTCAGT CCTTTGGGAC TGAACTGGCT
    GAAACAGAAC TACCAAATGA TGTGCAGTCA ACCAGCTCCC TTCTTGCAAT ACATACGGAA
    AAGAAGGACA AAATGAAGGA AGATATAAGG TTAGCAGTAG AACAGGGAGA CGATATCCTT
    GGAAGTATTA CAGAGCCAGT TACTGAGAAC CCTGAATACA AGCTGAATCA AGACCAACTA
    GACAATCAAA CTACAGTAGA AAGGCTTTTG GCTCAGTTAC ATGAAACAGA GTCCGCCTTT
    GATGAGTTTT GGATTAAGCA CCAGCAAAAA CTTGAGCAGT GTCTGCACCT TCGGAATTTT
    GAACAGAATT TTAGAGAGGT TAAAGCAGCT TTGGATATTG TGTCCGAGAG ACTGTCTGCT
    TTCACAGATG TGGGAAACAG CTGCTCACAT GTGGAGCACA TTTTGAAAGA TCTTGCCAAC
    TTTGAAGAAA AATCATGTGA AACTGTAGCA AAAGCCAGAA TGCTAGCTTC AGAGGGTGAT
    GCTTTTATTC AGAGCAATCA TTATGCTGTG GATTCCATTA TTCCAAAATG CAGTGAGCTT
    CATCATCTAT GTGATGCCTT CACTACTGAG ATGGAACAGA GGAGGAATGT TCTTAATAAA
    TCTCTAGAAC TGCATGGCTT ACTGGAAAAG TCTATGAAGT GGTGCGATGA AGGAATTTAT
    CTTCTGGCTT CACAACCTGT AGATAAGTGT CAGTCTCAGG ATGGTGCAGA ATCAGCATTA
    CAGGAGATTG AGAAATTCCT AGACACTGGT GCAGGCAATA AAATTAAGGA GTTGAATAAA
    GTTTACAGAG AGTATGCACA CATCCTCAAT GAGGACCTAA AGGAGCATAT ACAAAAGGTT
    TTCCAGAAGC AAGAAAGCAT GGAAGAAATG TTTCAAAAGA GACAAGTAAG TCTTAAGAAA
    CTGGCAGCTA AGCAAACACG TCCTGTTCAG CCAGTTGCAC CTAGACCCGA AGCCTTTGTA
    AAATCTCCTT GTACCTCTCC AGGTCTTCAA AGAGATTGTG TATCCAACAC AGAAAGCGGT
    GTGCTTCGGA AAGTAAACTA CAGAAGAGGA AAGAGTGAAA TGAGTGAACT CCATCAAAGC
    AGAGGAGGAT CTACAATGGA TGATGAAGAA AACCTAGCTG TGTTACGGCA GCATGTAATG
    AATGAACTTA TTGAAACGGA ACGAGCATAT GTGGAAGAAC TTCTCTGTGT TCTTGAGGGG
    TATGCTGCAG AAATGGACAA CCCCTTAATG GCTCACCTCA TTTCACCAGA ACTCCAAAAC
    AAGAAGGATA TTTTGTTTGG GAATATGGAA GAAATTTATC ACTTTCACAA CAGGATATTC
    TTGAGAGAAC TAGAAAATTA TGTAGAATAC CCAGAACTGG TAGGACGGTG CTTTCTGGAT
    CAGATGGAAG ATTTCCAGAT TTATGAGAAA TACTGTCAAA ACAAACCTCG ATCTGAAAGT
    TTGTGGAGGC AATTTTCAGA TTCCATATTC TTTCAGGAAT GTCAAAGGAA ACTCGATCAC
    AAGCTCAGTT TGGACTCATA CCTGCTGAAG CCAGTTCAAA GAATAACCAA ATACCAATTA
    TTGCTAAAGG AGATGCTGAA GTACAGTAAG AACTGTGAAG GTGCTGAAGA TCTTCAGGAG
    GCATTAAATT CTATATTGGG TATTCTCAAA GCAGTCAATG ATTCTATGCA CCAGATAGCC
    ATCACAGGCT ATGATGGAAA CCTGAATGAG CTGGGCAAGC TCCTAATGCA GGGATCCTTC
    AACGTCTGGA CTGATCATAA AAAGGGTCAC TCAAAGGTGA AAGATTTGGC ACGCTTCAAG
    CCAATGCAGC GACACCTGTT CCTCCACGAG AAAGCAGTGC TGTTCTGTAA AAAGCGAGAA
    GAAAATGGAG AAGGATATGA GAAGGCACCT TCTTATAGCT ATAAACACTC CTTAAATATG
    GCAGCAGTTG GAATAACAGA AAATGTCAAA GGTGATGCAA AAAAATTTGA AATCTGGTAT
    AATGCAAGGG AAGAAGTTTA CATCATACAG GCACCAACTC CTGAAGTTAA AGCCACTTGG
    GTAAATGAAA TAAGAAAAGT GCTGACTAGT CAACTGCAGG CATGCAGAGA AGCCAGTCAA
    CATAGGACAC TTGAACACAC TCAGAGTTTA CCTCTACCAG CATCATCCTG TACAAGTCCT
    TCAAGGAACA ACATCAGAAA CGTGAAAAAA ATGGATGAGA GAAAAGCTGA TCTCACAAGT
    CTAGAAGGTT ATGTCACCAC AGTAGCACCA AAGTACCCCG AGAAAGGCAA AGATGACACT
    AACTCTACCT CAGAATGCTC TGTACTGTCA AAAAAGCGCT TTACGCTGCA GGGCTTTACT
    AACCTCAAAA GTCAGAAAGC TTCTCCTACC AGTCCTGACA AAAAAGCTAA ACGGCACGAA
    GTAGTGAGTG ATCCAACTCC TTTTGGTGTA AGAGGTTGGG CTAAAACGTC TCATTCTCTT
    GATGCATCTG AAGAAAATGA TGGCTGGTCC AGTGCTGAGG AGCCGCTGAA TTCATCGGAT
    GCAGAAGAAG AAGGAGGAGG AGGAGGAGAG AGAAATGAGA TGAAGCTGAC TCCTGGTAAA
    TATACAGTTG TGTCTGACTA CGAGAAAGGA GTTTCTGAAG AATTTGCAGT GAAAGGTGGA
    GATCTAGTTC AACTGATTCG TGAAGGGGAG GAGGGACTTT GGTATGTGAA GAATCTGACC
    ACTGGTAGAG AAGGTTGGAT CCCAGCAAAC AATCTGCTGA TGCTCATAGG CAATTCAAAA
    TCAGCTCAAT CTTTGAGCAG CTCTGAATCA GGCACTGCTT CCAGCACTTT GAGCACGTCA
    TCAAGTTGCA GTGATAGCTG CAATGCCAGC AGCACCAGCT TCTCAGACAT TAAGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30671
    Clone ID Related Accession (Same CDS sequence) XM_015277845.1 , XM_015277845.2
    Accession Version XM_015277845.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCTGACT ATTGGTACTG GAGGCTGGTC CCGTGCAGAG AAACCGGCAG CTGTAACTGT 
    TTTGCAACAG CCAACCAATC TAATGAGGAG AAAGAAGAAG ACACGAACCA CCAGGTTGTG
    GTGTCAGACA TCATGCAGCT GGTGCGAGAC AAGCAGGAGG TGCCGGAAAG AACAGCAGAT
    GAAATCATGC ATCAGGATAT CATCCCCCTC TATGCAGCAG ATATTCATGA CCAGTTAAAG
    AAACAATTTG CTTATCTGTC AGGTGGAAGA GGGGGAGATG GATGCCCTGT TATCACTTTT
    CCCGACTACC CAGCTTTCAG CGAGATCCCT GAGAAGGAGT TCCAGAATGT CCTGACCTAC
    CTGACCAGCA TCCCGAGTTT ACGAGATGCT GGAATCGGGT TTATTCTTGT CATAGACCGC
    AGACAGGACA AATGGACTTC TGTGAAAGCT TCCATACTGC GGATAGCAGC ATCTTTCCCA
    GGGAACTTGC AACTCGTCCT CGTTCTGCGC CCAACAGGAC TTATTCATCG AGCTCTCTCA
    GACATTGCTT TCAAGTTCAA CAGAGATGAG TTTAAAATGA AAGTACCTAT CATAATGCTG
    GGCTCAGTAT CAGAACTGCA GGGTTACATT GATAAAACAC AATTAACAGA GGATCTTGGT
    GGCACCCTGG ATTACTGCCA TAACAGATGG CTATCCCTTC GCACTGCTAT AGAAGGTTTT
    GCCCAAAAGG TTAAGCAAAC AGCTCAGATT CTTCAGTCCT TTGGGACTGA ACTGGCTGAA
    ACAGAACTAC CAAATGATGT GCAGTCAACC AGCTCCCTTC TTGCAATACA TACGGAAAAG
    AAGGACAAAA TGAAGGAAGA TATAAGGTTA GCAGTAGAAC AGGGAGACGA TATCCTTGGA
    AGTATTACAG AGCCAGTTAC TGAGAACCCT GAATACAAGC TGAATCAAGA CCAACTAGAC
    AATCAAACTA CAGTAGAAAG GCTTTTGGCT CAGTTACATG AAACAGAGTC CGCCTTTGAT
    GAGTTTTGGA TTAAGCACCA GCAAAAACTT GAGCAGTGTC TGCACCTTCG GAATTTTGAA
    CAGAATTTTA GAGAGGTTAA AGCAGCTTTG GATATTGTGT CCGAGAGACT GTCTGCTTTC
    ACAGATGTGG GAAACAGCTG CTCACATGTG GAGCACATTT TGAAAGATCT TGCCAACTTT
    GAAGAAAAAT CATGTGAAAC TGTAGCAAAA GCCAGAATGC TAGCTTCAGA GGGTGATGCT
    TTTATTCAGA GCAATCATTA TGCTGTGGAT TCCATTATTC CAAAATGCAG TGAGCTTCAT
    CATCTATGTG ATGCCTTCAC TACTGAGATG GAACAGAGGA GGAATGTTCT TAATAAATCT
    CTAGAACTGC ATGGCTTACT GGAAAAGTCT ATGAAGTGGT GCGATGAAGG AATTTATCTT
    CTGGCTTCAC AACCTGTAGA TAAGTGTCAG TCTCAGGATG GTGCAGAATC AGCATTACAG
    GAGATTGAGA AATTCCTAGA CACTGGTGCA GGCAATAAAA TTAAGGAGTT GAATAAAGTT
    TACAGAGAGT ATGCACACAT CCTCAATGAG GACCTAAAGG AGCATATACA AAAGGTTTTC
    CAGAAGCAAG AAAGCATGGA AGAAATGTTT CAAAAGAGAC AAGTAAGTCT TAAGAAACTG
    GCAGCTAAGC AAACACGTCC TGTTCAGCCA GTTGCACCTA GACCCGAAGC CTTTGTAAAA
    TCTCCTTGTA CCTCTCCAGG TCTTCAAAGA GATTGTGTAT CCAACACAGA AAGCGGTGTG
    CTTCGGAAAG TAAACTACAG AAGAGGAAAG AGTGAAATGA GTGAACTCCA TCAAAGCAGA
    GGAGGATCTA CAATGGATGA TGAAGAAAAC CTAGCTGTGT TACGGCAGCA TGTAATGAAT
    GAACTTATTG AAACGGAACG AGCATATGTG GAAGAACTTC TCTGTGTTCT TGAGGGGTAT
    GCTGCAGAAA TGGACAACCC CTTAATGGCT CACCTCATTT CACCAGAACT CCAAAACAAG
    AAGGATATTT TGTTTGGGAA TATGGAAGAA ATTTATCACT TTCACAACAG GATATTCTTG
    AGAGAACTAG AAAATTATGT AGAATACCCA GAACTGGTAG GACGGTGCTT TCTGGATCAG
    ATGGAAGATT TCCAGATTTA TGAGAAATAC TGTCAAAACA AACCTCGATC TGAAAGTTTG
    TGGAGGCAAT TTTCAGATTC CATATTCTTT CAGGAATGTC AAAGGAAACT CGATCACAAG
    CTCAGTTTGG ACTCATACCT GCTGAAGCCA GTTCAAAGAA TAACCAAATA CCAATTATTG
    CTAAAGGAGA TGCTGAAGTA CAGTAAGAAC TGTGAAGGTG CTGAAGATCT TCAGGAGGCA
    TTAAATTCTA TATTGGGTAT TCTCAAAGCA GTCAATGATT CTATGCACCA GATAGCCATC
    ACAGGCTATG ATGGAAACCT GAATGAGCTG GGCAAGCTCC TAATGCAGGG ATCCTTCAAC
    GTCTGGACTG ATCATAAAAA GGGTCACTCA AAGGTGAAAG ATTTGGCACG CTTCAAGCCA
    ATGCAGCGAC ACCTGTTCCT CCACGAGAAA GCAGTGCTGT TCTGTAAAAA GCGAGAAGAA
    AATGGAGAAG GATATGAGAA GGCACCTTCT TATAGCTATA AACACTCCTT AAATATGGCA
    GCAGTTGGAA TAACAGAAAA TGTCAAAGGT GATGCAAAAA AATTTGAAAT CTGGTATAAT
    GCAAGGGAAG AAGTTTACAT CATACAGGCA CCAACTCCTG AAGTTAAAGC CACTTGGGTA
    AATGAAATAA GAAAAGTGCT GACTAGTCAA CTGCAGGCAT GCAGAGAAGC CAGTCAACAT
    AGGACACTTG AACACACTCA GAGTTTACCT CTACCAGCAT CATCCTGTAC AAGTCCTTCA
    AGGAACAACA TCAGAAACGT GAAAAAAATG GATGAGAGAA AAGCTGATCT CACAAGTCTA
    GAAGGTTATG TCACCACAGT AGCACCAAAG TACCCCGAGA AAGGCAAAGC TTCTCCTACC
    AGTCCTGACA AAAAAGCTAA ACGGCACGAA GTAGTGAGTG ATCCAACTCC TTTTGGTGTA
    AGAGGTTGGG CTAAAACGTC TCATTCTCTT GATGCATCTG AAGAAAATGA TGGCTGGTCC
    AGTGCTGAGG AGCCGCTGAA TTCATCGGAT GCAGAAGAAG AAGGAGGAGG AGGAGGAGAG
    AGAAATGAGA TGAAGCTGAC TCCTGGTAAA TATACAGTTG TGTCTGACTA CGAGAAAGGA
    GTTTCTGAAG AATTTGCAGT GAAAGGTGGA GATCTAGTTC AACTGATTCG TGAAGGGGAG
    GAGGGACTTT GGTATGTGAA GAATCTGACC ACTGGTAGAG AAGGTTGGAT CCCAGCAAAC
    AATCTGCTGA TGCTCATAGG CAATTCAAAA TCAGCTCAAT CTTTGAGCAG CTCTGAATCA
    GGCACTGCTT CCAGCACTTT GAGCACGTCA TCAAGTTGCA GTGATAGCTG CAATGCCAGC
    AGCACCAGCT TCTCAGACAT TAAGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133331.1
    CDS116..3685
    Translation

    Target ORF information:

    RefSeq Version XM_015277845.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277845.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCTGACT ATTGGTACTG GAGGCTGGTC CCGTGCAGAG AAACCGGCAG CTGTAACTGT 
    TTTGCAACAG CCAACCAATC TAATGAGGAG AAAGAAGAAG ACACGAACCA CCAGGTTGTG
    GTGTCAGACA TCATGCAGCT GGTGCGAGAC AAGCAGGAGG TGCCGGAAAG AACAGCAGAT
    GAAATCATGC ATCAGGATAT CATCCCCCTC TATGCAGCAG ATATTCATGA CCAGTTAAAG
    AAACAATTTG CTTATCTGTC AGGTGGAAGA GGGGGAGATG GATGCCCTGT TATCACTTTT
    CCCGACTACC CAGCTTTCAG CGAGATCCCT GAGAAGGAGT TCCAGAATGT CCTGACCTAC
    CTGACCAGCA TCCCGAGTTT ACGAGATGCT GGAATCGGGT TTATTCTTGT CATAGACCGC
    AGACAGGACA AATGGACTTC TGTGAAAGCT TCCATACTGC GGATAGCAGC ATCTTTCCCA
    GGGAACTTGC AACTCGTCCT CGTTCTGCGC CCAACAGGAC TTATTCATCG AGCTCTCTCA
    GACATTGCTT TCAAGTTCAA CAGAGATGAG TTTAAAATGA AAGTACCTAT CATAATGCTG
    GGCTCAGTAT CAGAACTGCA GGGTTACATT GATAAAACAC AATTAACAGA GGATCTTGGT
    GGCACCCTGG ATTACTGCCA TAACAGATGG CTATCCCTTC GCACTGCTAT AGAAGGTTTT
    GCCCAAAAGG TTAAGCAAAC AGCTCAGATT CTTCAGTCCT TTGGGACTGA ACTGGCTGAA
    ACAGAACTAC CAAATGATGT GCAGTCAACC AGCTCCCTTC TTGCAATACA TACGGAAAAG
    AAGGACAAAA TGAAGGAAGA TATAAGGTTA GCAGTAGAAC AGGGAGACGA TATCCTTGGA
    AGTATTACAG AGCCAGTTAC TGAGAACCCT GAATACAAGC TGAATCAAGA CCAACTAGAC
    AATCAAACTA CAGTAGAAAG GCTTTTGGCT CAGTTACATG AAACAGAGTC CGCCTTTGAT
    GAGTTTTGGA TTAAGCACCA GCAAAAACTT GAGCAGTGTC TGCACCTTCG GAATTTTGAA
    CAGAATTTTA GAGAGGTTAA AGCAGCTTTG GATATTGTGT CCGAGAGACT GTCTGCTTTC
    ACAGATGTGG GAAACAGCTG CTCACATGTG GAGCACATTT TGAAAGATCT TGCCAACTTT
    GAAGAAAAAT CATGTGAAAC TGTAGCAAAA GCCAGAATGC TAGCTTCAGA GGGTGATGCT
    TTTATTCAGA GCAATCATTA TGCTGTGGAT TCCATTATTC CAAAATGCAG TGAGCTTCAT
    CATCTATGTG ATGCCTTCAC TACTGAGATG GAACAGAGGA GGAATGTTCT TAATAAATCT
    CTAGAACTGC ATGGCTTACT GGAAAAGTCT ATGAAGTGGT GCGATGAAGG AATTTATCTT
    CTGGCTTCAC AACCTGTAGA TAAGTGTCAG TCTCAGGATG GTGCAGAATC AGCATTACAG
    GAGATTGAGA AATTCCTAGA CACTGGTGCA GGCAATAAAA TTAAGGAGTT GAATAAAGTT
    TACAGAGAGT ATGCACACAT CCTCAATGAG GACCTAAAGG AGCATATACA AAAGGTTTTC
    CAGAAGCAAG AAAGCATGGA AGAAATGTTT CAAAAGAGAC AAGTAAGTCT TAAGAAACTG
    GCAGCTAAGC AAACACGTCC TGTTCAGCCA GTTGCACCTA GACCCGAAGC CTTTGTAAAA
    TCTCCTTGTA CCTCTCCAGG TCTTCAAAGA GATTGTGTAT CCAACACAGA AAGCGGTGTG
    CTTCGGAAAG TAAACTACAG AAGAGGAAAG AGTGAAATGA GTGAACTCCA TCAAAGCAGA
    GGAGGATCTA CAATGGATGA TGAAGAAAAC CTAGCTGTGT TACGGCAGCA TGTAATGAAT
    GAACTTATTG AAACGGAACG AGCATATGTG GAAGAACTTC TCTGTGTTCT TGAGGGGTAT
    GCTGCAGAAA TGGACAACCC CTTAATGGCT CACCTCATTT CACCAGAACT CCAAAACAAG
    AAGGATATTT TGTTTGGGAA TATGGAAGAA ATTTATCACT TTCACAACAG GATATTCTTG
    AGAGAACTAG AAAATTATGT AGAATACCCA GAACTGGTAG GACGGTGCTT TCTGGATCAG
    ATGGAAGATT TCCAGATTTA TGAGAAATAC TGTCAAAACA AACCTCGATC TGAAAGTTTG
    TGGAGGCAAT TTTCAGATTC CATATTCTTT CAGGAATGTC AAAGGAAACT CGATCACAAG
    CTCAGTTTGG ACTCATACCT GCTGAAGCCA GTTCAAAGAA TAACCAAATA CCAATTATTG
    CTAAAGGAGA TGCTGAAGTA CAGTAAGAAC TGTGAAGGTG CTGAAGATCT TCAGGAGGCA
    TTAAATTCTA TATTGGGTAT TCTCAAAGCA GTCAATGATT CTATGCACCA GATAGCCATC
    ACAGGCTATG ATGGAAACCT GAATGAGCTG GGCAAGCTCC TAATGCAGGG ATCCTTCAAC
    GTCTGGACTG ATCATAAAAA GGGTCACTCA AAGGTGAAAG ATTTGGCACG CTTCAAGCCA
    ATGCAGCGAC ACCTGTTCCT CCACGAGAAA GCAGTGCTGT TCTGTAAAAA GCGAGAAGAA
    AATGGAGAAG GATATGAGAA GGCACCTTCT TATAGCTATA AACACTCCTT AAATATGGCA
    GCAGTTGGAA TAACAGAAAA TGTCAAAGGT GATGCAAAAA AATTTGAAAT CTGGTATAAT
    GCAAGGGAAG AAGTTTACAT CATACAGGCA CCAACTCCTG AAGTTAAAGC CACTTGGGTA
    AATGAAATAA GAAAAGTGCT GACTAGTCAA CTGCAGGCAT GCAGAGAAGC CAGTCAACAT
    AGGACACTTG AACACACTCA GAGTTTACCT CTACCAGCAT CATCCTGTAC AAGTCCTTCA
    AGGAACAACA TCAGAAACGT GAAAAAAATG GATGAGAGAA AAGCTGATCT CACAAGTCTA
    GAAGGTTATG TCACCACAGT AGCACCAAAG TACCCCGAGA AAGGCAAAGC TTCTCCTACC
    AGTCCTGACA AAAAAGCTAA ACGGCACGAA GTAGTGAGTG ATCCAACTCC TTTTGGTGTA
    AGAGGTTGGG CTAAAACGTC TCATTCTCTT GATGCATCTG AAGAAAATGA TGGCTGGTCC
    AGTGCTGAGG AGCCGCTGAA TTCATCGGAT GCAGAAGAAG AAGGAGGAGG AGGAGGAGAG
    AGAAATGAGA TGAAGCTGAC TCCTGGTAAA TATACAGTTG TGTCTGACTA CGAGAAAGGA
    GTTTCTGAAG AATTTGCAGT GAAAGGTGGA GATCTAGTTC AACTGATTCG TGAAGGGGAG
    GAGGGACTTT GGTATGTGAA GAATCTGACC ACTGGTAGAG AAGGTTGGAT CCCAGCAAAC
    AATCTGCTGA TGCTCATAGG CAATTCAAAA TCAGCTCAAT CTTTGAGCAG CTCTGAATCA
    GGCACTGCTT CCAGCACTTT GAGCACGTCA TCAAGTTGCA GTGATAGCTG CAATGCCAGC
    AGCACCAGCT TCTCAGACAT TAAGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30671
    Clone ID Related Accession (Same CDS sequence) XM_015277845.1 , XM_015277845.2
    Accession Version XM_015277845.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277845.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCTGACT ATTGGTACTG GAGGCTGGTC CCGTGCAGAG AAACCGGCAG CTGTAACTGT 
    TTTGCAACAG CCAACCAATC TAATGAGGAG AAAGAAGAAG ACACGAACCA CCAGGTTGTG
    GTGTCAGACA TCATGCAGCT GGTGCGAGAC AAGCAGGAGG TGCCGGAAAG AACAGCAGAT
    GAAATCATGC ATCAGGATAT CATCCCCCTC TATGCAGCAG ATATTCATGA CCAGTTAAAG
    AAACAATTTG CTTATCTGTC AGGTGGAAGA GGGGGAGATG GATGCCCTGT TATCACTTTT
    CCCGACTACC CAGCTTTCAG CGAGATCCCT GAGAAGGAGT TCCAGAATGT CCTGACCTAC
    CTGACCAGCA TCCCGAGTTT ACGAGATGCT GGAATCGGGT TTATTCTTGT CATAGACCGC
    AGACAGGACA AATGGACTTC TGTGAAAGCT TCCATACTGC GGATAGCAGC ATCTTTCCCA
    GGGAACTTGC AACTCGTCCT CGTTCTGCGC CCAACAGGAC TTATTCATCG AGCTCTCTCA
    GACATTGCTT TCAAGTTCAA CAGAGATGAG TTTAAAATGA AAGTACCTAT CATAATGCTG
    GGCTCAGTAT CAGAACTGCA GGGTTACATT GATAAAACAC AATTAACAGA GGATCTTGGT
    GGCACCCTGG ATTACTGCCA TAACAGATGG CTATCCCTTC GCACTGCTAT AGAAGGTTTT
    GCCCAAAAGG TTAAGCAAAC AGCTCAGATT CTTCAGTCCT TTGGGACTGA ACTGGCTGAA
    ACAGAACTAC CAAATGATGT GCAGTCAACC AGCTCCCTTC TTGCAATACA TACGGAAAAG
    AAGGACAAAA TGAAGGAAGA TATAAGGTTA GCAGTAGAAC AGGGAGACGA TATCCTTGGA
    AGTATTACAG AGCCAGTTAC TGAGAACCCT GAATACAAGC TGAATCAAGA CCAACTAGAC
    AATCAAACTA CAGTAGAAAG GCTTTTGGCT CAGTTACATG AAACAGAGTC CGCCTTTGAT
    GAGTTTTGGA TTAAGCACCA GCAAAAACTT GAGCAGTGTC TGCACCTTCG GAATTTTGAA
    CAGAATTTTA GAGAGGTTAA AGCAGCTTTG GATATTGTGT CCGAGAGACT GTCTGCTTTC
    ACAGATGTGG GAAACAGCTG CTCACATGTG GAGCACATTT TGAAAGATCT TGCCAACTTT
    GAAGAAAAAT CATGTGAAAC TGTAGCAAAA GCCAGAATGC TAGCTTCAGA GGGTGATGCT
    TTTATTCAGA GCAATCATTA TGCTGTGGAT TCCATTATTC CAAAATGCAG TGAGCTTCAT
    CATCTATGTG ATGCCTTCAC TACTGAGATG GAACAGAGGA GGAATGTTCT TAATAAATCT
    CTAGAACTGC ATGGCTTACT GGAAAAGTCT ATGAAGTGGT GCGATGAAGG AATTTATCTT
    CTGGCTTCAC AACCTGTAGA TAAGTGTCAG TCTCAGGATG GTGCAGAATC AGCATTACAG
    GAGATTGAGA AATTCCTAGA CACTGGTGCA GGCAATAAAA TTAAGGAGTT GAATAAAGTT
    TACAGAGAGT ATGCACACAT CCTCAATGAG GACCTAAAGG AGCATATACA AAAGGTTTTC
    CAGAAGCAAG AAAGCATGGA AGAAATGTTT CAAAAGAGAC AAGTAAGTCT TAAGAAACTG
    GCAGCTAAGC AAACACGTCC TGTTCAGCCA GTTGCACCTA GACCCGAAGC CTTTGTAAAA
    TCTCCTTGTA CCTCTCCAGG TCTTCAAAGA GATTGTGTAT CCAACACAGA AAGCGGTGTG
    CTTCGGAAAG TAAACTACAG AAGAGGAAAG AGTGAAATGA GTGAACTCCA TCAAAGCAGA
    GGAGGATCTA CAATGGATGA TGAAGAAAAC CTAGCTGTGT TACGGCAGCA TGTAATGAAT
    GAACTTATTG AAACGGAACG AGCATATGTG GAAGAACTTC TCTGTGTTCT TGAGGGGTAT
    GCTGCAGAAA TGGACAACCC CTTAATGGCT CACCTCATTT CACCAGAACT CCAAAACAAG
    AAGGATATTT TGTTTGGGAA TATGGAAGAA ATTTATCACT TTCACAACAG GATATTCTTG
    AGAGAACTAG AAAATTATGT AGAATACCCA GAACTGGTAG GACGGTGCTT TCTGGATCAG
    ATGGAAGATT TCCAGATTTA TGAGAAATAC TGTCAAAACA AACCTCGATC TGAAAGTTTG
    TGGAGGCAAT TTTCAGATTC CATATTCTTT CAGGAATGTC AAAGGAAACT CGATCACAAG
    CTCAGTTTGG ACTCATACCT GCTGAAGCCA GTTCAAAGAA TAACCAAATA CCAATTATTG
    CTAAAGGAGA TGCTGAAGTA CAGTAAGAAC TGTGAAGGTG CTGAAGATCT TCAGGAGGCA
    TTAAATTCTA TATTGGGTAT TCTCAAAGCA GTCAATGATT CTATGCACCA GATAGCCATC
    ACAGGCTATG ATGGAAACCT GAATGAGCTG GGCAAGCTCC TAATGCAGGG ATCCTTCAAC
    GTCTGGACTG ATCATAAAAA GGGTCACTCA AAGGTGAAAG ATTTGGCACG CTTCAAGCCA
    ATGCAGCGAC ACCTGTTCCT CCACGAGAAA GCAGTGCTGT TCTGTAAAAA GCGAGAAGAA
    AATGGAGAAG GATATGAGAA GGCACCTTCT TATAGCTATA AACACTCCTT AAATATGGCA
    GCAGTTGGAA TAACAGAAAA TGTCAAAGGT GATGCAAAAA AATTTGAAAT CTGGTATAAT
    GCAAGGGAAG AAGTTTACAT CATACAGGCA CCAACTCCTG AAGTTAAAGC CACTTGGGTA
    AATGAAATAA GAAAAGTGCT GACTAGTCAA CTGCAGGCAT GCAGAGAAGC CAGTCAACAT
    AGGACACTTG AACACACTCA GAGTTTACCT CTACCAGCAT CATCCTGTAC AAGTCCTTCA
    AGGAACAACA TCAGAAACGT GAAAAAAATG GATGAGAGAA AAGCTGATCT CACAAGTCTA
    GAAGGTTATG TCACCACAGT AGCACCAAAG TACCCCGAGA AAGGCAAAGC TTCTCCTACC
    AGTCCTGACA AAAAAGCTAA ACGGCACGAA GTAGTGAGTG ATCCAACTCC TTTTGGTGTA
    AGAGGTTGGG CTAAAACGTC TCATTCTCTT GATGCATCTG AAGAAAATGA TGGCTGGTCC
    AGTGCTGAGG AGCCGCTGAA TTCATCGGAT GCAGAAGAAG AAGGAGGAGG AGGAGGAGAG
    AGAAATGAGA TGAAGCTGAC TCCTGGTAAA TATACAGTTG TGTCTGACTA CGAGAAAGGA
    GTTTCTGAAG AATTTGCAGT GAAAGGTGGA GATCTAGTTC AACTGATTCG TGAAGGGGAG
    GAGGGACTTT GGTATGTGAA GAATCTGACC ACTGGTAGAG AAGGTTGGAT CCCAGCAAAC
    AATCTGCTGA TGCTCATAGG CAATTCAAAA TCAGCTCAAT CTTTGAGCAG CTCTGAATCA
    GGCACTGCTT CCAGCACTTT GAGCACGTCA TCAAGTTGCA GTGATAGCTG CAATGCCAGC
    AGCACCAGCT TCTCAGACAT TAAGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133331.1
    CDS130..3699
    Translation

    Target ORF information:

    RefSeq Version XM_015277845.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277845.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCTGACT ATTGGTACTG GAGGCTGGTC CCGTGCAGAG AAACCGGCAG CTGTAACTGT 
    TTTGCAACAG CCAACCAATC TAATGAGGAG AAAGAAGAAG ACACGAACCA CCAGGTTGTG
    GTGTCAGACA TCATGCAGCT GGTGCGAGAC AAGCAGGAGG TGCCGGAAAG AACAGCAGAT
    GAAATCATGC ATCAGGATAT CATCCCCCTC TATGCAGCAG ATATTCATGA CCAGTTAAAG
    AAACAATTTG CTTATCTGTC AGGTGGAAGA GGGGGAGATG GATGCCCTGT TATCACTTTT
    CCCGACTACC CAGCTTTCAG CGAGATCCCT GAGAAGGAGT TCCAGAATGT CCTGACCTAC
    CTGACCAGCA TCCCGAGTTT ACGAGATGCT GGAATCGGGT TTATTCTTGT CATAGACCGC
    AGACAGGACA AATGGACTTC TGTGAAAGCT TCCATACTGC GGATAGCAGC ATCTTTCCCA
    GGGAACTTGC AACTCGTCCT CGTTCTGCGC CCAACAGGAC TTATTCATCG AGCTCTCTCA
    GACATTGCTT TCAAGTTCAA CAGAGATGAG TTTAAAATGA AAGTACCTAT CATAATGCTG
    GGCTCAGTAT CAGAACTGCA GGGTTACATT GATAAAACAC AATTAACAGA GGATCTTGGT
    GGCACCCTGG ATTACTGCCA TAACAGATGG CTATCCCTTC GCACTGCTAT AGAAGGTTTT
    GCCCAAAAGG TTAAGCAAAC AGCTCAGATT CTTCAGTCCT TTGGGACTGA ACTGGCTGAA
    ACAGAACTAC CAAATGATGT GCAGTCAACC AGCTCCCTTC TTGCAATACA TACGGAAAAG
    AAGGACAAAA TGAAGGAAGA TATAAGGTTA GCAGTAGAAC AGGGAGACGA TATCCTTGGA
    AGTATTACAG AGCCAGTTAC TGAGAACCCT GAATACAAGC TGAATCAAGA CCAACTAGAC
    AATCAAACTA CAGTAGAAAG GCTTTTGGCT CAGTTACATG AAACAGAGTC CGCCTTTGAT
    GAGTTTTGGA TTAAGCACCA GCAAAAACTT GAGCAGTGTC TGCACCTTCG GAATTTTGAA
    CAGAATTTTA GAGAGGTTAA AGCAGCTTTG GATATTGTGT CCGAGAGACT GTCTGCTTTC
    ACAGATGTGG GAAACAGCTG CTCACATGTG GAGCACATTT TGAAAGATCT TGCCAACTTT
    GAAGAAAAAT CATGTGAAAC TGTAGCAAAA GCCAGAATGC TAGCTTCAGA GGGTGATGCT
    TTTATTCAGA GCAATCATTA TGCTGTGGAT TCCATTATTC CAAAATGCAG TGAGCTTCAT
    CATCTATGTG ATGCCTTCAC TACTGAGATG GAACAGAGGA GGAATGTTCT TAATAAATCT
    CTAGAACTGC ATGGCTTACT GGAAAAGTCT ATGAAGTGGT GCGATGAAGG AATTTATCTT
    CTGGCTTCAC AACCTGTAGA TAAGTGTCAG TCTCAGGATG GTGCAGAATC AGCATTACAG
    GAGATTGAGA AATTCCTAGA CACTGGTGCA GGCAATAAAA TTAAGGAGTT GAATAAAGTT
    TACAGAGAGT ATGCACACAT CCTCAATGAG GACCTAAAGG AGCATATACA AAAGGTTTTC
    CAGAAGCAAG AAAGCATGGA AGAAATGTTT CAAAAGAGAC AAGTAAGTCT TAAGAAACTG
    GCAGCTAAGC AAACACGTCC TGTTCAGCCA GTTGCACCTA GACCCGAAGC CTTTGTAAAA
    TCTCCTTGTA CCTCTCCAGG TCTTCAAAGA GATTGTGTAT CCAACACAGA AAGCGGTGTG
    CTTCGGAAAG TAAACTACAG AAGAGGAAAG AGTGAAATGA GTGAACTCCA TCAAAGCAGA
    GGAGGATCTA CAATGGATGA TGAAGAAAAC CTAGCTGTGT TACGGCAGCA TGTAATGAAT
    GAACTTATTG AAACGGAACG AGCATATGTG GAAGAACTTC TCTGTGTTCT TGAGGGGTAT
    GCTGCAGAAA TGGACAACCC CTTAATGGCT CACCTCATTT CACCAGAACT CCAAAACAAG
    AAGGATATTT TGTTTGGGAA TATGGAAGAA ATTTATCACT TTCACAACAG GATATTCTTG
    AGAGAACTAG AAAATTATGT AGAATACCCA GAACTGGTAG GACGGTGCTT TCTGGATCAG
    ATGGAAGATT TCCAGATTTA TGAGAAATAC TGTCAAAACA AACCTCGATC TGAAAGTTTG
    TGGAGGCAAT TTTCAGATTC CATATTCTTT CAGGAATGTC AAAGGAAACT CGATCACAAG
    CTCAGTTTGG ACTCATACCT GCTGAAGCCA GTTCAAAGAA TAACCAAATA CCAATTATTG
    CTAAAGGAGA TGCTGAAGTA CAGTAAGAAC TGTGAAGGTG CTGAAGATCT TCAGGAGGCA
    TTAAATTCTA TATTGGGTAT TCTCAAAGCA GTCAATGATT CTATGCACCA GATAGCCATC
    ACAGGCTATG ATGGAAACCT GAATGAGCTG GGCAAGCTCC TAATGCAGGG ATCCTTCAAC
    GTCTGGACTG ATCATAAAAA GGGTCACTCA AAGGTGAAAG ATTTGGCACG CTTCAAGCCA
    ATGCAGCGAC ACCTGTTCCT CCACGAGAAA GCAGTGCTGT TCTGTAAAAA GCGAGAAGAA
    AATGGAGAAG GATATGAGAA GGCACCTTCT TATAGCTATA AACACTCCTT AAATATGGCA
    GCAGTTGGAA TAACAGAAAA TGTCAAAGGT GATGCAAAAA AATTTGAAAT CTGGTATAAT
    GCAAGGGAAG AAGTTTACAT CATACAGGCA CCAACTCCTG AAGTTAAAGC CACTTGGGTA
    AATGAAATAA GAAAAGTGCT GACTAGTCAA CTGCAGGCAT GCAGAGAAGC CAGTCAACAT
    AGGACACTTG AACACACTCA GAGTTTACCT CTACCAGCAT CATCCTGTAC AAGTCCTTCA
    AGGAACAACA TCAGAAACGT GAAAAAAATG GATGAGAGAA AAGCTGATCT CACAAGTCTA
    GAAGGTTATG TCACCACAGT AGCACCAAAG TACCCCGAGA AAGGCAAAGC TTCTCCTACC
    AGTCCTGACA AAAAAGCTAA ACGGCACGAA GTAGTGAGTG ATCCAACTCC TTTTGGTGTA
    AGAGGTTGGG CTAAAACGTC TCATTCTCTT GATGCATCTG AAGAAAATGA TGGCTGGTCC
    AGTGCTGAGG AGCCGCTGAA TTCATCGGAT GCAGAAGAAG AAGGAGGAGG AGGAGGAGAG
    AGAAATGAGA TGAAGCTGAC TCCTGGTAAA TATACAGTTG TGTCTGACTA CGAGAAAGGA
    GTTTCTGAAG AATTTGCAGT GAAAGGTGGA GATCTAGTTC AACTGATTCG TGAAGGGGAG
    GAGGGACTTT GGTATGTGAA GAATCTGACC ACTGGTAGAG AAGGTTGGAT CCCAGCAAAC
    AATCTGCTGA TGCTCATAGG CAATTCAAAA TCAGCTCAAT CTTTGAGCAG CTCTGAATCA
    GGCACTGCTT CCAGCACTTT GAGCACGTCA TCAAGTTGCA GTGATAGCTG CAATGCCAGC
    AGCACCAGCT TCTCAGACAT TAAGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30672
    Clone ID Related Accession (Same CDS sequence) XM_015277846.1
    Accession Version XM_015277846.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTGTGTC ATGTATGCTG CTCATACATA ATCAAAGGAG ATGGGCTGCG TGCATTGGAT 
    TTTGGACAGA ACAATAAGAA AGATGAAATC ATGCATCAGG ATATCATCCC CCTCTATGCA
    GCAGATATTC ATGACCAGTT AAAGAAACAA TTTGCTTATC TGTCAGGTGG AAGAGGGGGA
    GATGGATGCC CTGTTATCAC TTTTCCCGAC TACCCAGCTT TCAGCGAGAT CCCTGAGAAG
    GAGTTCCAGA ATGTCCTGAC CTACCTGACC AGCATCCCGA GTTTACGAGA TGCTGGAATC
    GGGTTTATTC TTGTCATAGA CCGCAGACAG GACAAATGGA CTTCTGTGAA AGCTTCCATA
    CTGCGGATAG CAGCATCTTT CCCAGGGAAC TTGCAACTCG TCCTCGTTCT GCGCCCAACA
    GGACTTATTC ATCGAGCTCT CTCAGACATT GCTTTCAAGT TCAACAGAGA TGAGTTTAAA
    ATGAAAGTAC CTATCATAAT GCTGGGCTCA GTATCAGAAC TGCAGGGTTA CATTGATAAA
    ACACAATTAA CAGAGGATCT TGGTGGCACC CTGGATTACT GCCATAACAG ATGGCTATCC
    CTTCGCACTG CTATAGAAGG TTTTGCCCAA AAGGTTAAGC AAACAGCTCA GATTCTTCAG
    TCCTTTGGGA CTGAACTGGC TGAAACAGAA CTACCAAATG ATGTGCAGTC AACCAGCTCC
    CTTCTTGCAA TACATACGGA AAAGAAGGAC AAAATGAAGG AAGATATAAG GTTAGCAGTA
    GAACAGGGAG ACGATATCCT TGGAAGTATT ACAGAGCCAG TTACTGAGAA CCCTGAATAC
    AAGCTGAATC AAGACCAACT AGACAATCAA ACTACAGTAG AAAGGCTTTT GGCTCAGTTA
    CATGAAACAG AGTCCGCCTT TGATGAGTTT TGGATTAAGC ACCAGCAAAA ACTTGAGCAG
    TGTCTGCACC TTCGGAATTT TGAACAGAAT TTTAGAGAGG TTAAAGCAGC TTTGGATATT
    GTGTCCGAGA GACTGTCTGC TTTCACAGAT GTGGGAAACA GCTGCTCACA TGTGGAGCAC
    ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA AAATCATGTG AAACTGTAGC AAAAGCCAGA
    ATGCTAGCTT CAGAGGGTGA TGCTTTTATT CAGAGCAATC ATTATGCTGT GGATTCCATT
    ATTCCAAAAT GCAGTGAGCT TCATCATCTA TGTGATGCCT TCACTACTGA GATGGAACAG
    AGGAGGAATG TTCTTAATAA ATCTCTAGAA CTGCATGGCT TACTGGAAAA GTCTATGAAG
    TGGTGCGATG AAGGAATTTA TCTTCTGGCT TCACAACCTG TAGATAAGTG TCAGTCTCAG
    GATGGTGCAG AATCAGCATT ACAGGAGATT GAGAAATTCC TAGACACTGG TGCAGGCAAT
    AAAATTAAGG AGTTGAATAA AGTTTACAGA GAGTATGCAC ACATCCTCAA TGAGGACCTA
    AAGGAGCATA TACAAAAGGT TTTCCAGAAG CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG
    AGACAAGTAA GTCTTAAGAA ACTGGCAGCT AAGCAAACAC GTCCTGTTCA GCCAGTTGCA
    CCTAGACCCG AAGCCTTTGT AAAATCTCCT TGTACCTCTC CAGGTCTTCA AAGAGATTGT
    GTATCCAACA CAGAAAGCGG TGTGCTTCGG AAAGTAAACT ACAGAAGAGG AAAGAGTGAA
    ATGAGTGAAC TCCATCAAAG CAGAGGAGGA TCTACAATGG ATGATGAAGA AAACCTAGCT
    GTGTTACGGC AGCATGTAAT GAATGAACTT ATTGAAACGG AACGAGCATA TGTGGAAGAA
    CTTCTCTGTG TTCTTGAGGG GTATGCTGCA GAAATGGACA ACCCCTTAAT GGCTCACCTC
    ATTTCACCAG AACTCCAAAA CAAGAAGGAT ATTTTGTTTG GGAATATGGA AGAAATTTAT
    CACTTTCACA ACAGGATATT CTTGAGAGAA CTAGAAAATT ATGTAGAATA CCCAGAACTG
    GTAGGACGGT GCTTTCTGGA TCAGATGGAA GATTTCCAGA TTTATGAGAA ATACTGTCAA
    AACAAACCTC GATCTGAAAG TTTGTGGAGG CAATTTTCAG ATTCCATATT CTTTCAGGAA
    TGTCAAAGGA AACTCGATCA CAAGCTCAGT TTGGACTCAT ACCTGCTGAA GCCAGTTCAA
    AGAATAACCA AATACCAATT ATTGCTAAAG GAGATGCTGA AGTACAGTAA GAACTGTGAA
    GGTGCTGAAG ATCTTCAGGA GGCATTAAAT TCTATATTGG GTATTCTCAA AGCAGTCAAT
    GATTCTATGC ACCAGATAGC CATCACAGGC TATGATGGAA ACCTGAATGA GCTGGGCAAG
    CTCCTAATGC AGGGATCCTT CAACGTCTGG ACTGATCATA AAAAGGGTCA CTCAAAGGTG
    AAAGATTTGG CACGCTTCAA GCCAATGCAG CGACACCTGT TCCTCCACGA GAAAGCAGTG
    CTGTTCTGTA AAAAGCGAGA AGAAAATGGA GAAGGATATG AGAAGGCACC TTCTTATAGC
    TATAAACACT CCTTAAATAT GGCAGCAGTT GGAATAACAG AAAATGTCAA AGGTGATGCA
    AAAAAATTTG AAATCTGGTA TAATGCAAGG GAAGAAGTTT ACATCATACA GGCACCAACT
    CCTGAAGTTA AAGCCACTTG GGTAAATGAA ATAAGAAAAG TGCTGACTAG TCAACTGCAG
    GCATGCAGAG AAGCCAGTCA ACATAGGACA CTTGAACACA CTCAGAGTTT ACCTCTACCA
    GCATCATCCT GTACAAGTCC TTCAAGGAAC AACATCAGAA ACGTGAAAAA AATGGATGAG
    AGAAAAGCTG ATCTCACAAG TCTAGAAGGT TATGTCACCA CAGTAGCACC AAAGTACCCC
    GAGAAAGGCA AAGATGACAC TAACTCTACC TCAGAATGCT CTGTACTGTC AAAAAAGCGC
    TTTACGCTGC AGGGCTTTAC TAACCTCAAA AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC
    AAAAAAGCTA AACGGCACGA AGTAGTGAGT GATCCAACTC CTTTTGGTGT AAGAGGTTGG
    GCTAAAACGT CTCATTCTCT TGATGCATCT GAAGAAAATG ATGGCTGGTC CAGTGCTGAG
    GAGCCGCTGA ATTCATCGGA TGCAGAAGAA GAAGGAGGAG GAGGAGGAGA GAGAAATGAG
    ATGAAGCTGA CTCCTGGTAA ATATACAGTT GTGTCTGACT ACGAGAAAGG AGTTTCTGAA
    GAATTTGCAG TGAAAGGTGG AGATCTAGTT CAACTGATTC GTGAAGGGGA GGAGGGACTT
    TGGTATGTGA AGAATCTGAC CACTGGTAGA GAAGGTTGGA TCCCAGCAAA CAATCTGCTG
    ATGCTCATAG GCAATTCAAA ATCAGCTCAA TCTTTGAGCA GCTCTGAATC AGGCACTGCT
    TCCAGCACTT TGAGCACGTC ATCAAGTTGC AGTGATAGCT GCAATGCCAG CAGCACCAGC
    TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133332.1
    CDS16..3576
    Translation

    Target ORF information:

    RefSeq Version XM_015277846.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277846.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTGTGTC ATGTATGCTG CTCATACATA ATCAAAGGAG ATGGGCTGCG TGCATTGGAT 
    TTTGGACAGA ACAATAAGAA AGATGAAATC ATGCATCAGG ATATCATCCC CCTCTATGCA
    GCAGATATTC ATGACCAGTT AAAGAAACAA TTTGCTTATC TGTCAGGTGG AAGAGGGGGA
    GATGGATGCC CTGTTATCAC TTTTCCCGAC TACCCAGCTT TCAGCGAGAT CCCTGAGAAG
    GAGTTCCAGA ATGTCCTGAC CTACCTGACC AGCATCCCGA GTTTACGAGA TGCTGGAATC
    GGGTTTATTC TTGTCATAGA CCGCAGACAG GACAAATGGA CTTCTGTGAA AGCTTCCATA
    CTGCGGATAG CAGCATCTTT CCCAGGGAAC TTGCAACTCG TCCTCGTTCT GCGCCCAACA
    GGACTTATTC ATCGAGCTCT CTCAGACATT GCTTTCAAGT TCAACAGAGA TGAGTTTAAA
    ATGAAAGTAC CTATCATAAT GCTGGGCTCA GTATCAGAAC TGCAGGGTTA CATTGATAAA
    ACACAATTAA CAGAGGATCT TGGTGGCACC CTGGATTACT GCCATAACAG ATGGCTATCC
    CTTCGCACTG CTATAGAAGG TTTTGCCCAA AAGGTTAAGC AAACAGCTCA GATTCTTCAG
    TCCTTTGGGA CTGAACTGGC TGAAACAGAA CTACCAAATG ATGTGCAGTC AACCAGCTCC
    CTTCTTGCAA TACATACGGA AAAGAAGGAC AAAATGAAGG AAGATATAAG GTTAGCAGTA
    GAACAGGGAG ACGATATCCT TGGAAGTATT ACAGAGCCAG TTACTGAGAA CCCTGAATAC
    AAGCTGAATC AAGACCAACT AGACAATCAA ACTACAGTAG AAAGGCTTTT GGCTCAGTTA
    CATGAAACAG AGTCCGCCTT TGATGAGTTT TGGATTAAGC ACCAGCAAAA ACTTGAGCAG
    TGTCTGCACC TTCGGAATTT TGAACAGAAT TTTAGAGAGG TTAAAGCAGC TTTGGATATT
    GTGTCCGAGA GACTGTCTGC TTTCACAGAT GTGGGAAACA GCTGCTCACA TGTGGAGCAC
    ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA AAATCATGTG AAACTGTAGC AAAAGCCAGA
    ATGCTAGCTT CAGAGGGTGA TGCTTTTATT CAGAGCAATC ATTATGCTGT GGATTCCATT
    ATTCCAAAAT GCAGTGAGCT TCATCATCTA TGTGATGCCT TCACTACTGA GATGGAACAG
    AGGAGGAATG TTCTTAATAA ATCTCTAGAA CTGCATGGCT TACTGGAAAA GTCTATGAAG
    TGGTGCGATG AAGGAATTTA TCTTCTGGCT TCACAACCTG TAGATAAGTG TCAGTCTCAG
    GATGGTGCAG AATCAGCATT ACAGGAGATT GAGAAATTCC TAGACACTGG TGCAGGCAAT
    AAAATTAAGG AGTTGAATAA AGTTTACAGA GAGTATGCAC ACATCCTCAA TGAGGACCTA
    AAGGAGCATA TACAAAAGGT TTTCCAGAAG CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG
    AGACAAGTAA GTCTTAAGAA ACTGGCAGCT AAGCAAACAC GTCCTGTTCA GCCAGTTGCA
    CCTAGACCCG AAGCCTTTGT AAAATCTCCT TGTACCTCTC CAGGTCTTCA AAGAGATTGT
    GTATCCAACA CAGAAAGCGG TGTGCTTCGG AAAGTAAACT ACAGAAGAGG AAAGAGTGAA
    ATGAGTGAAC TCCATCAAAG CAGAGGAGGA TCTACAATGG ATGATGAAGA AAACCTAGCT
    GTGTTACGGC AGCATGTAAT GAATGAACTT ATTGAAACGG AACGAGCATA TGTGGAAGAA
    CTTCTCTGTG TTCTTGAGGG GTATGCTGCA GAAATGGACA ACCCCTTAAT GGCTCACCTC
    ATTTCACCAG AACTCCAAAA CAAGAAGGAT ATTTTGTTTG GGAATATGGA AGAAATTTAT
    CACTTTCACA ACAGGATATT CTTGAGAGAA CTAGAAAATT ATGTAGAATA CCCAGAACTG
    GTAGGACGGT GCTTTCTGGA TCAGATGGAA GATTTCCAGA TTTATGAGAA ATACTGTCAA
    AACAAACCTC GATCTGAAAG TTTGTGGAGG CAATTTTCAG ATTCCATATT CTTTCAGGAA
    TGTCAAAGGA AACTCGATCA CAAGCTCAGT TTGGACTCAT ACCTGCTGAA GCCAGTTCAA
    AGAATAACCA AATACCAATT ATTGCTAAAG GAGATGCTGA AGTACAGTAA GAACTGTGAA
    GGTGCTGAAG ATCTTCAGGA GGCATTAAAT TCTATATTGG GTATTCTCAA AGCAGTCAAT
    GATTCTATGC ACCAGATAGC CATCACAGGC TATGATGGAA ACCTGAATGA GCTGGGCAAG
    CTCCTAATGC AGGGATCCTT CAACGTCTGG ACTGATCATA AAAAGGGTCA CTCAAAGGTG
    AAAGATTTGG CACGCTTCAA GCCAATGCAG CGACACCTGT TCCTCCACGA GAAAGCAGTG
    CTGTTCTGTA AAAAGCGAGA AGAAAATGGA GAAGGATATG AGAAGGCACC TTCTTATAGC
    TATAAACACT CCTTAAATAT GGCAGCAGTT GGAATAACAG AAAATGTCAA AGGTGATGCA
    AAAAAATTTG AAATCTGGTA TAATGCAAGG GAAGAAGTTT ACATCATACA GGCACCAACT
    CCTGAAGTTA AAGCCACTTG GGTAAATGAA ATAAGAAAAG TGCTGACTAG TCAACTGCAG
    GCATGCAGAG AAGCCAGTCA ACATAGGACA CTTGAACACA CTCAGAGTTT ACCTCTACCA
    GCATCATCCT GTACAAGTCC TTCAAGGAAC AACATCAGAA ACGTGAAAAA AATGGATGAG
    AGAAAAGCTG ATCTCACAAG TCTAGAAGGT TATGTCACCA CAGTAGCACC AAAGTACCCC
    GAGAAAGGCA AAGATGACAC TAACTCTACC TCAGAATGCT CTGTACTGTC AAAAAAGCGC
    TTTACGCTGC AGGGCTTTAC TAACCTCAAA AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC
    AAAAAAGCTA AACGGCACGA AGTAGTGAGT GATCCAACTC CTTTTGGTGT AAGAGGTTGG
    GCTAAAACGT CTCATTCTCT TGATGCATCT GAAGAAAATG ATGGCTGGTC CAGTGCTGAG
    GAGCCGCTGA ATTCATCGGA TGCAGAAGAA GAAGGAGGAG GAGGAGGAGA GAGAAATGAG
    ATGAAGCTGA CTCCTGGTAA ATATACAGTT GTGTCTGACT ACGAGAAAGG AGTTTCTGAA
    GAATTTGCAG TGAAAGGTGG AGATCTAGTT CAACTGATTC GTGAAGGGGA GGAGGGACTT
    TGGTATGTGA AGAATCTGAC CACTGGTAGA GAAGGTTGGA TCCCAGCAAA CAATCTGCTG
    ATGCTCATAG GCAATTCAAA ATCAGCTCAA TCTTTGAGCA GCTCTGAATC AGGCACTGCT
    TCCAGCACTT TGAGCACGTC ATCAAGTTGC AGTGATAGCT GCAATGCCAG CAGCACCAGC
    TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30673
    Clone ID Related Accession (Same CDS sequence) XM_015277847.1 , XM_015277847.2
    Accession Version XM_015277847.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGATGCT GGCTGCACGC TGAAGAAATG GCCCTAGAAG AATTGATGCA AAGATTAAAT 
    GCGGTTTCCC AGTGCACTGA TGAAATCATG CATCAGGATA TCATCCCCCT CTATGCAGCA
    GATATTCATG ACCAGTTAAA GAAACAATTT GCTTATCTGT CAGGTGGAAG AGGGGGAGAT
    GGATGCCCTG TTATCACTTT TCCCGACTAC CCAGCTTTCA GCGAGATCCC TGAGAAGGAG
    TTCCAGAATG TCCTGACCTA CCTGACCAGC ATCCCGAGTT TACGAGATGC TGGAATCGGG
    TTTATTCTTG TCATAGACCG CAGACAGGAC AAATGGACTT CTGTGAAAGC TTCCATACTG
    CGGATAGCAG CATCTTTCCC AGGGAACTTG CAACTCGTCC TCGTTCTGCG CCCAACAGGA
    CTTATTCATC GAGCTCTCTC AGACATTGCT TTCAAGTTCA ACAGAGATGA GTTTAAAATG
    AAAGTACCTA TCATAATGCT GGGCTCAGTA TCAGAACTGC AGGGTTACAT TGATAAAACA
    CAATTAACAG AGGATCTTGG TGGCACCCTG GATTACTGCC ATAACAGATG GCTATCCCTT
    CGCACTGCTA TAGAAGGTTT TGCCCAAAAG GTTAAGCAAA CAGCTCAGAT TCTTCAGTCC
    TTTGGGACTG AACTGGCTGA AACAGAACTA CCAAATGATG TGCAGTCAAC CAGCTCCCTT
    CTTGCAATAC ATACGGAAAA GAAGGACAAA ATGAAGGAAG ATATAAGGTT AGCAGTAGAA
    CAGGGAGACG ATATCCTTGG AAGTATTACA GAGCCAGTTA CTGAGAACCC TGAATACAAG
    CTGAATCAAG ACCAACTAGA CAATCAAACT ACAGTAGAAA GGCTTTTGGC TCAGTTACAT
    GAAACAGAGT CCGCCTTTGA TGAGTTTTGG ATTAAGCACC AGCAAAAACT TGAGCAGTGT
    CTGCACCTTC GGAATTTTGA ACAGAATTTT AGAGAGGTTA AAGCAGCTTT GGATATTGTG
    TCCGAGAGAC TGTCTGCTTT CACAGATGTG GGAAACAGCT GCTCACATGT GGAGCACATT
    TTGAAAGATC TTGCCAACTT TGAAGAAAAA TCATGTGAAA CTGTAGCAAA AGCCAGAATG
    CTAGCTTCAG AGGGTGATGC TTTTATTCAG AGCAATCATT ATGCTGTGGA TTCCATTATT
    CCAAAATGCA GTGAGCTTCA TCATCTATGT GATGCCTTCA CTACTGAGAT GGAACAGAGG
    AGGAATGTTC TTAATAAATC TCTAGAACTG CATGGCTTAC TGGAAAAGTC TATGAAGTGG
    TGCGATGAAG GAATTTATCT TCTGGCTTCA CAACCTGTAG ATAAGTGTCA GTCTCAGGAT
    GGTGCAGAAT CAGCATTACA GGAGATTGAG AAATTCCTAG ACACTGGTGC AGGCAATAAA
    ATTAAGGAGT TGAATAAAGT TTACAGAGAG TATGCACACA TCCTCAATGA GGACCTAAAG
    GAGCATATAC AAAAGGTTTT CCAGAAGCAA GAAAGCATGG AAGAAATGTT TCAAAAGAGA
    CAAGTAAGTC TTAAGAAACT GGCAGCTAAG CAAACACGTC CTGTTCAGCC AGTTGCACCT
    AGACCCGAAG CCTTTGTAAA ATCTCCTTGT ACCTCTCCAG GTCTTCAAAG AGATTGTGTA
    TCCAACACAG AAAGCGGTGT GCTTCGGAAA GTAAACTACA GAAGAGGAAA GAGTGAAATG
    AGTGAACTCC ATCAAAGCAG AGGAGGATCT ACAATGGATG ATGAAGAAAA CCTAGCTGTG
    TTACGGCAGC ATGTAATGAA TGAACTTATT GAAACGGAAC GAGCATATGT GGAAGAACTT
    CTCTGTGTTC TTGAGGGGTA TGCTGCAGAA ATGGACAACC CCTTAATGGC TCACCTCATT
    TCACCAGAAC TCCAAAACAA GAAGGATATT TTGTTTGGGA ATATGGAAGA AATTTATCAC
    TTTCACAACA GGATATTCTT GAGAGAACTA GAAAATTATG TAGAATACCC AGAACTGGTA
    GGACGGTGCT TTCTGGATCA GATGGAAGAT TTCCAGATTT ATGAGAAATA CTGTCAAAAC
    AAACCTCGAT CTGAAAGTTT GTGGAGGCAA TTTTCAGATT CCATATTCTT TCAGGAATGT
    CAAAGGAAAC TCGATCACAA GCTCAGTTTG GACTCATACC TGCTGAAGCC AGTTCAAAGA
    ATAACCAAAT ACCAATTATT GCTAAAGGAG ATGCTGAAGT ACAGTAAGAA CTGTGAAGGT
    GCTGAAGATC TTCAGGAGGC ATTAAATTCT ATATTGGGTA TTCTCAAAGC AGTCAATGAT
    TCTATGCACC AGATAGCCAT CACAGGCTAT GATGGAAACC TGAATGAGCT GGGCAAGCTC
    CTAATGCAGG GATCCTTCAA CGTCTGGACT GATCATAAAA AGGGTCACTC AAAGGTGAAA
    GATTTGGCAC GCTTCAAGCC AATGCAGCGA CACCTGTTCC TCCACGAGAA AGCAGTGCTG
    TTCTGTAAAA AGCGAGAAGA AAATGGAGAA GGATATGAGA AGGCACCTTC TTATAGCTAT
    AAACACTCCT TAAATATGGC AGCAGTTGGA ATAACAGAAA ATGTCAAAGG TGATGCAAAA
    AAATTTGAAA TCTGGTATAA TGCAAGGGAA GAAGTTTACA TCATACAGGC ACCAACTCCT
    GAAGTTAAAG CCACTTGGGT AAATGAAATA AGAAAAGTGC TGACTAGTCA ACTGCAGGCA
    TGCAGAGAAG CCAGTCAACA TAGGACACTT GAACACACTC AGAGTTTACC TCTACCAGCA
    TCATCCTGTA CAAGTCCTTC AAGGAACAAC ATCAGAAACG TGAAAAAAAT GGATGAGAGA
    AAAGCTGATC TCACAAGTCT AGAAGGTTAT GTCACCACAG TAGCACCAAA GTACCCCGAG
    AAAGGCAAAG ATGACACTAA CTCTACCTCA GAATGCTCTG TACTGTCAAA AAAGCGCTTT
    ACGCTGCAGG GCTTTACTAA CCTCAAAAGT CAGAAAGCTT CTCCTACCAG TCCTGACAAA
    AAAGCTAAAC GGCACGAAGT AGTGAGTGAT CCAACTCCTT TTGGTGTAAG AGGTTGGGCT
    AAAACGTCTC ATTCTCTTGA TGCATCTGAA GAAAATGATG GCTGGTCCAG TGCTGAGGAG
    CCGCTGAATT CATCGGATGC AGAAGAAGAA GGAGGAGGAG GAGGAGAGAG AAATGAGATG
    AAGCTGACTC CTGGTAAATA TACAGTTGTG TCTGACTACG AGAAAGGAGT TTCTGAAGAA
    TTTGCAGTGA AAGGTGGAGA TCTAGTTCAA CTGATTCGTG AAGGGGAGGA GGGACTTTGG
    TATGTGAAGA ATCTGACCAC TGGTAGAGAA GGTTGGATCC CAGCAAACAA TCTGCTGATG
    CTCATAGGCA ATTCAAAATC AGCTCAATCT TTGAGCAGCT CTGAATCAGG CACTGCTTCC
    AGCACTTTGA GCACGTCATC AAGTTGCAGT GATAGCTGCA ATGCCAGCAG CACCAGCTTC
    TCAGACATTA AGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133333.1
    CDS539..4096
    Translation

    Target ORF information:

    RefSeq Version XM_015277847.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277847.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGATGCT GGCTGCACGC TGAAGAAATG GCCCTAGAAG AATTGATGCA AAGATTAAAT 
    GCGGTTTCCC AGTGCACTGA TGAAATCATG CATCAGGATA TCATCCCCCT CTATGCAGCA
    GATATTCATG ACCAGTTAAA GAAACAATTT GCTTATCTGT CAGGTGGAAG AGGGGGAGAT
    GGATGCCCTG TTATCACTTT TCCCGACTAC CCAGCTTTCA GCGAGATCCC TGAGAAGGAG
    TTCCAGAATG TCCTGACCTA CCTGACCAGC ATCCCGAGTT TACGAGATGC TGGAATCGGG
    TTTATTCTTG TCATAGACCG CAGACAGGAC AAATGGACTT CTGTGAAAGC TTCCATACTG
    CGGATAGCAG CATCTTTCCC AGGGAACTTG CAACTCGTCC TCGTTCTGCG CCCAACAGGA
    CTTATTCATC GAGCTCTCTC AGACATTGCT TTCAAGTTCA ACAGAGATGA GTTTAAAATG
    AAAGTACCTA TCATAATGCT GGGCTCAGTA TCAGAACTGC AGGGTTACAT TGATAAAACA
    CAATTAACAG AGGATCTTGG TGGCACCCTG GATTACTGCC ATAACAGATG GCTATCCCTT
    CGCACTGCTA TAGAAGGTTT TGCCCAAAAG GTTAAGCAAA CAGCTCAGAT TCTTCAGTCC
    TTTGGGACTG AACTGGCTGA AACAGAACTA CCAAATGATG TGCAGTCAAC CAGCTCCCTT
    CTTGCAATAC ATACGGAAAA GAAGGACAAA ATGAAGGAAG ATATAAGGTT AGCAGTAGAA
    CAGGGAGACG ATATCCTTGG AAGTATTACA GAGCCAGTTA CTGAGAACCC TGAATACAAG
    CTGAATCAAG ACCAACTAGA CAATCAAACT ACAGTAGAAA GGCTTTTGGC TCAGTTACAT
    GAAACAGAGT CCGCCTTTGA TGAGTTTTGG ATTAAGCACC AGCAAAAACT TGAGCAGTGT
    CTGCACCTTC GGAATTTTGA ACAGAATTTT AGAGAGGTTA AAGCAGCTTT GGATATTGTG
    TCCGAGAGAC TGTCTGCTTT CACAGATGTG GGAAACAGCT GCTCACATGT GGAGCACATT
    TTGAAAGATC TTGCCAACTT TGAAGAAAAA TCATGTGAAA CTGTAGCAAA AGCCAGAATG
    CTAGCTTCAG AGGGTGATGC TTTTATTCAG AGCAATCATT ATGCTGTGGA TTCCATTATT
    CCAAAATGCA GTGAGCTTCA TCATCTATGT GATGCCTTCA CTACTGAGAT GGAACAGAGG
    AGGAATGTTC TTAATAAATC TCTAGAACTG CATGGCTTAC TGGAAAAGTC TATGAAGTGG
    TGCGATGAAG GAATTTATCT TCTGGCTTCA CAACCTGTAG ATAAGTGTCA GTCTCAGGAT
    GGTGCAGAAT CAGCATTACA GGAGATTGAG AAATTCCTAG ACACTGGTGC AGGCAATAAA
    ATTAAGGAGT TGAATAAAGT TTACAGAGAG TATGCACACA TCCTCAATGA GGACCTAAAG
    GAGCATATAC AAAAGGTTTT CCAGAAGCAA GAAAGCATGG AAGAAATGTT TCAAAAGAGA
    CAAGTAAGTC TTAAGAAACT GGCAGCTAAG CAAACACGTC CTGTTCAGCC AGTTGCACCT
    AGACCCGAAG CCTTTGTAAA ATCTCCTTGT ACCTCTCCAG GTCTTCAAAG AGATTGTGTA
    TCCAACACAG AAAGCGGTGT GCTTCGGAAA GTAAACTACA GAAGAGGAAA GAGTGAAATG
    AGTGAACTCC ATCAAAGCAG AGGAGGATCT ACAATGGATG ATGAAGAAAA CCTAGCTGTG
    TTACGGCAGC ATGTAATGAA TGAACTTATT GAAACGGAAC GAGCATATGT GGAAGAACTT
    CTCTGTGTTC TTGAGGGGTA TGCTGCAGAA ATGGACAACC CCTTAATGGC TCACCTCATT
    TCACCAGAAC TCCAAAACAA GAAGGATATT TTGTTTGGGA ATATGGAAGA AATTTATCAC
    TTTCACAACA GGATATTCTT GAGAGAACTA GAAAATTATG TAGAATACCC AGAACTGGTA
    GGACGGTGCT TTCTGGATCA GATGGAAGAT TTCCAGATTT ATGAGAAATA CTGTCAAAAC
    AAACCTCGAT CTGAAAGTTT GTGGAGGCAA TTTTCAGATT CCATATTCTT TCAGGAATGT
    CAAAGGAAAC TCGATCACAA GCTCAGTTTG GACTCATACC TGCTGAAGCC AGTTCAAAGA
    ATAACCAAAT ACCAATTATT GCTAAAGGAG ATGCTGAAGT ACAGTAAGAA CTGTGAAGGT
    GCTGAAGATC TTCAGGAGGC ATTAAATTCT ATATTGGGTA TTCTCAAAGC AGTCAATGAT
    TCTATGCACC AGATAGCCAT CACAGGCTAT GATGGAAACC TGAATGAGCT GGGCAAGCTC
    CTAATGCAGG GATCCTTCAA CGTCTGGACT GATCATAAAA AGGGTCACTC AAAGGTGAAA
    GATTTGGCAC GCTTCAAGCC AATGCAGCGA CACCTGTTCC TCCACGAGAA AGCAGTGCTG
    TTCTGTAAAA AGCGAGAAGA AAATGGAGAA GGATATGAGA AGGCACCTTC TTATAGCTAT
    AAACACTCCT TAAATATGGC AGCAGTTGGA ATAACAGAAA ATGTCAAAGG TGATGCAAAA
    AAATTTGAAA TCTGGTATAA TGCAAGGGAA GAAGTTTACA TCATACAGGC ACCAACTCCT
    GAAGTTAAAG CCACTTGGGT AAATGAAATA AGAAAAGTGC TGACTAGTCA ACTGCAGGCA
    TGCAGAGAAG CCAGTCAACA TAGGACACTT GAACACACTC AGAGTTTACC TCTACCAGCA
    TCATCCTGTA CAAGTCCTTC AAGGAACAAC ATCAGAAACG TGAAAAAAAT GGATGAGAGA
    AAAGCTGATC TCACAAGTCT AGAAGGTTAT GTCACCACAG TAGCACCAAA GTACCCCGAG
    AAAGGCAAAG ATGACACTAA CTCTACCTCA GAATGCTCTG TACTGTCAAA AAAGCGCTTT
    ACGCTGCAGG GCTTTACTAA CCTCAAAAGT CAGAAAGCTT CTCCTACCAG TCCTGACAAA
    AAAGCTAAAC GGCACGAAGT AGTGAGTGAT CCAACTCCTT TTGGTGTAAG AGGTTGGGCT
    AAAACGTCTC ATTCTCTTGA TGCATCTGAA GAAAATGATG GCTGGTCCAG TGCTGAGGAG
    CCGCTGAATT CATCGGATGC AGAAGAAGAA GGAGGAGGAG GAGGAGAGAG AAATGAGATG
    AAGCTGACTC CTGGTAAATA TACAGTTGTG TCTGACTACG AGAAAGGAGT TTCTGAAGAA
    TTTGCAGTGA AAGGTGGAGA TCTAGTTCAA CTGATTCGTG AAGGGGAGGA GGGACTTTGG
    TATGTGAAGA ATCTGACCAC TGGTAGAGAA GGTTGGATCC CAGCAAACAA TCTGCTGATG
    CTCATAGGCA ATTCAAAATC AGCTCAATCT TTGAGCAGCT CTGAATCAGG CACTGCTTCC
    AGCACTTTGA GCACGTCATC AAGTTGCAGT GATAGCTGCA ATGCCAGCAG CACCAGCTTC
    TCAGACATTA AGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30673
    Clone ID Related Accession (Same CDS sequence) XM_015277847.1 , XM_015277847.2
    Accession Version XM_015277847.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277847.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGATGCT GGCTGCACGC TGAAGAAATG GCCCTAGAAG AATTGATGCA AAGATTAAAT 
    GCGGTTTCCC AGTGCACTGA TGAAATCATG CATCAGGATA TCATCCCCCT CTATGCAGCA
    GATATTCATG ACCAGTTAAA GAAACAATTT GCTTATCTGT CAGGTGGAAG AGGGGGAGAT
    GGATGCCCTG TTATCACTTT TCCCGACTAC CCAGCTTTCA GCGAGATCCC TGAGAAGGAG
    TTCCAGAATG TCCTGACCTA CCTGACCAGC ATCCCGAGTT TACGAGATGC TGGAATCGGG
    TTTATTCTTG TCATAGACCG CAGACAGGAC AAATGGACTT CTGTGAAAGC TTCCATACTG
    CGGATAGCAG CATCTTTCCC AGGGAACTTG CAACTCGTCC TCGTTCTGCG CCCAACAGGA
    CTTATTCATC GAGCTCTCTC AGACATTGCT TTCAAGTTCA ACAGAGATGA GTTTAAAATG
    AAAGTACCTA TCATAATGCT GGGCTCAGTA TCAGAACTGC AGGGTTACAT TGATAAAACA
    CAATTAACAG AGGATCTTGG TGGCACCCTG GATTACTGCC ATAACAGATG GCTATCCCTT
    CGCACTGCTA TAGAAGGTTT TGCCCAAAAG GTTAAGCAAA CAGCTCAGAT TCTTCAGTCC
    TTTGGGACTG AACTGGCTGA AACAGAACTA CCAAATGATG TGCAGTCAAC CAGCTCCCTT
    CTTGCAATAC ATACGGAAAA GAAGGACAAA ATGAAGGAAG ATATAAGGTT AGCAGTAGAA
    CAGGGAGACG ATATCCTTGG AAGTATTACA GAGCCAGTTA CTGAGAACCC TGAATACAAG
    CTGAATCAAG ACCAACTAGA CAATCAAACT ACAGTAGAAA GGCTTTTGGC TCAGTTACAT
    GAAACAGAGT CCGCCTTTGA TGAGTTTTGG ATTAAGCACC AGCAAAAACT TGAGCAGTGT
    CTGCACCTTC GGAATTTTGA ACAGAATTTT AGAGAGGTTA AAGCAGCTTT GGATATTGTG
    TCCGAGAGAC TGTCTGCTTT CACAGATGTG GGAAACAGCT GCTCACATGT GGAGCACATT
    TTGAAAGATC TTGCCAACTT TGAAGAAAAA TCATGTGAAA CTGTAGCAAA AGCCAGAATG
    CTAGCTTCAG AGGGTGATGC TTTTATTCAG AGCAATCATT ATGCTGTGGA TTCCATTATT
    CCAAAATGCA GTGAGCTTCA TCATCTATGT GATGCCTTCA CTACTGAGAT GGAACAGAGG
    AGGAATGTTC TTAATAAATC TCTAGAACTG CATGGCTTAC TGGAAAAGTC TATGAAGTGG
    TGCGATGAAG GAATTTATCT TCTGGCTTCA CAACCTGTAG ATAAGTGTCA GTCTCAGGAT
    GGTGCAGAAT CAGCATTACA GGAGATTGAG AAATTCCTAG ACACTGGTGC AGGCAATAAA
    ATTAAGGAGT TGAATAAAGT TTACAGAGAG TATGCACACA TCCTCAATGA GGACCTAAAG
    GAGCATATAC AAAAGGTTTT CCAGAAGCAA GAAAGCATGG AAGAAATGTT TCAAAAGAGA
    CAAGTAAGTC TTAAGAAACT GGCAGCTAAG CAAACACGTC CTGTTCAGCC AGTTGCACCT
    AGACCCGAAG CCTTTGTAAA ATCTCCTTGT ACCTCTCCAG GTCTTCAAAG AGATTGTGTA
    TCCAACACAG AAAGCGGTGT GCTTCGGAAA GTAAACTACA GAAGAGGAAA GAGTGAAATG
    AGTGAACTCC ATCAAAGCAG AGGAGGATCT ACAATGGATG ATGAAGAAAA CCTAGCTGTG
    TTACGGCAGC ATGTAATGAA TGAACTTATT GAAACGGAAC GAGCATATGT GGAAGAACTT
    CTCTGTGTTC TTGAGGGGTA TGCTGCAGAA ATGGACAACC CCTTAATGGC TCACCTCATT
    TCACCAGAAC TCCAAAACAA GAAGGATATT TTGTTTGGGA ATATGGAAGA AATTTATCAC
    TTTCACAACA GGATATTCTT GAGAGAACTA GAAAATTATG TAGAATACCC AGAACTGGTA
    GGACGGTGCT TTCTGGATCA GATGGAAGAT TTCCAGATTT ATGAGAAATA CTGTCAAAAC
    AAACCTCGAT CTGAAAGTTT GTGGAGGCAA TTTTCAGATT CCATATTCTT TCAGGAATGT
    CAAAGGAAAC TCGATCACAA GCTCAGTTTG GACTCATACC TGCTGAAGCC AGTTCAAAGA
    ATAACCAAAT ACCAATTATT GCTAAAGGAG ATGCTGAAGT ACAGTAAGAA CTGTGAAGGT
    GCTGAAGATC TTCAGGAGGC ATTAAATTCT ATATTGGGTA TTCTCAAAGC AGTCAATGAT
    TCTATGCACC AGATAGCCAT CACAGGCTAT GATGGAAACC TGAATGAGCT GGGCAAGCTC
    CTAATGCAGG GATCCTTCAA CGTCTGGACT GATCATAAAA AGGGTCACTC AAAGGTGAAA
    GATTTGGCAC GCTTCAAGCC AATGCAGCGA CACCTGTTCC TCCACGAGAA AGCAGTGCTG
    TTCTGTAAAA AGCGAGAAGA AAATGGAGAA GGATATGAGA AGGCACCTTC TTATAGCTAT
    AAACACTCCT TAAATATGGC AGCAGTTGGA ATAACAGAAA ATGTCAAAGG TGATGCAAAA
    AAATTTGAAA TCTGGTATAA TGCAAGGGAA GAAGTTTACA TCATACAGGC ACCAACTCCT
    GAAGTTAAAG CCACTTGGGT AAATGAAATA AGAAAAGTGC TGACTAGTCA ACTGCAGGCA
    TGCAGAGAAG CCAGTCAACA TAGGACACTT GAACACACTC AGAGTTTACC TCTACCAGCA
    TCATCCTGTA CAAGTCCTTC AAGGAACAAC ATCAGAAACG TGAAAAAAAT GGATGAGAGA
    AAAGCTGATC TCACAAGTCT AGAAGGTTAT GTCACCACAG TAGCACCAAA GTACCCCGAG
    AAAGGCAAAG ATGACACTAA CTCTACCTCA GAATGCTCTG TACTGTCAAA AAAGCGCTTT
    ACGCTGCAGG GCTTTACTAA CCTCAAAAGT CAGAAAGCTT CTCCTACCAG TCCTGACAAA
    AAAGCTAAAC GGCACGAAGT AGTGAGTGAT CCAACTCCTT TTGGTGTAAG AGGTTGGGCT
    AAAACGTCTC ATTCTCTTGA TGCATCTGAA GAAAATGATG GCTGGTCCAG TGCTGAGGAG
    CCGCTGAATT CATCGGATGC AGAAGAAGAA GGAGGAGGAG GAGGAGAGAG AAATGAGATG
    AAGCTGACTC CTGGTAAATA TACAGTTGTG TCTGACTACG AGAAAGGAGT TTCTGAAGAA
    TTTGCAGTGA AAGGTGGAGA TCTAGTTCAA CTGATTCGTG AAGGGGAGGA GGGACTTTGG
    TATGTGAAGA ATCTGACCAC TGGTAGAGAA GGTTGGATCC CAGCAAACAA TCTGCTGATG
    CTCATAGGCA ATTCAAAATC AGCTCAATCT TTGAGCAGCT CTGAATCAGG CACTGCTTCC
    AGCACTTTGA GCACGTCATC AAGTTGCAGT GATAGCTGCA ATGCCAGCAG CACCAGCTTC
    TCAGACATTA AGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133333.1
    CDS2494..6051
    Translation

    Target ORF information:

    RefSeq Version XM_015277847.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277847.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGATGCT GGCTGCACGC TGAAGAAATG GCCCTAGAAG AATTGATGCA AAGATTAAAT 
    GCGGTTTCCC AGTGCACTGA TGAAATCATG CATCAGGATA TCATCCCCCT CTATGCAGCA
    GATATTCATG ACCAGTTAAA GAAACAATTT GCTTATCTGT CAGGTGGAAG AGGGGGAGAT
    GGATGCCCTG TTATCACTTT TCCCGACTAC CCAGCTTTCA GCGAGATCCC TGAGAAGGAG
    TTCCAGAATG TCCTGACCTA CCTGACCAGC ATCCCGAGTT TACGAGATGC TGGAATCGGG
    TTTATTCTTG TCATAGACCG CAGACAGGAC AAATGGACTT CTGTGAAAGC TTCCATACTG
    CGGATAGCAG CATCTTTCCC AGGGAACTTG CAACTCGTCC TCGTTCTGCG CCCAACAGGA
    CTTATTCATC GAGCTCTCTC AGACATTGCT TTCAAGTTCA ACAGAGATGA GTTTAAAATG
    AAAGTACCTA TCATAATGCT GGGCTCAGTA TCAGAACTGC AGGGTTACAT TGATAAAACA
    CAATTAACAG AGGATCTTGG TGGCACCCTG GATTACTGCC ATAACAGATG GCTATCCCTT
    CGCACTGCTA TAGAAGGTTT TGCCCAAAAG GTTAAGCAAA CAGCTCAGAT TCTTCAGTCC
    TTTGGGACTG AACTGGCTGA AACAGAACTA CCAAATGATG TGCAGTCAAC CAGCTCCCTT
    CTTGCAATAC ATACGGAAAA GAAGGACAAA ATGAAGGAAG ATATAAGGTT AGCAGTAGAA
    CAGGGAGACG ATATCCTTGG AAGTATTACA GAGCCAGTTA CTGAGAACCC TGAATACAAG
    CTGAATCAAG ACCAACTAGA CAATCAAACT ACAGTAGAAA GGCTTTTGGC TCAGTTACAT
    GAAACAGAGT CCGCCTTTGA TGAGTTTTGG ATTAAGCACC AGCAAAAACT TGAGCAGTGT
    CTGCACCTTC GGAATTTTGA ACAGAATTTT AGAGAGGTTA AAGCAGCTTT GGATATTGTG
    TCCGAGAGAC TGTCTGCTTT CACAGATGTG GGAAACAGCT GCTCACATGT GGAGCACATT
    TTGAAAGATC TTGCCAACTT TGAAGAAAAA TCATGTGAAA CTGTAGCAAA AGCCAGAATG
    CTAGCTTCAG AGGGTGATGC TTTTATTCAG AGCAATCATT ATGCTGTGGA TTCCATTATT
    CCAAAATGCA GTGAGCTTCA TCATCTATGT GATGCCTTCA CTACTGAGAT GGAACAGAGG
    AGGAATGTTC TTAATAAATC TCTAGAACTG CATGGCTTAC TGGAAAAGTC TATGAAGTGG
    TGCGATGAAG GAATTTATCT TCTGGCTTCA CAACCTGTAG ATAAGTGTCA GTCTCAGGAT
    GGTGCAGAAT CAGCATTACA GGAGATTGAG AAATTCCTAG ACACTGGTGC AGGCAATAAA
    ATTAAGGAGT TGAATAAAGT TTACAGAGAG TATGCACACA TCCTCAATGA GGACCTAAAG
    GAGCATATAC AAAAGGTTTT CCAGAAGCAA GAAAGCATGG AAGAAATGTT TCAAAAGAGA
    CAAGTAAGTC TTAAGAAACT GGCAGCTAAG CAAACACGTC CTGTTCAGCC AGTTGCACCT
    AGACCCGAAG CCTTTGTAAA ATCTCCTTGT ACCTCTCCAG GTCTTCAAAG AGATTGTGTA
    TCCAACACAG AAAGCGGTGT GCTTCGGAAA GTAAACTACA GAAGAGGAAA GAGTGAAATG
    AGTGAACTCC ATCAAAGCAG AGGAGGATCT ACAATGGATG ATGAAGAAAA CCTAGCTGTG
    TTACGGCAGC ATGTAATGAA TGAACTTATT GAAACGGAAC GAGCATATGT GGAAGAACTT
    CTCTGTGTTC TTGAGGGGTA TGCTGCAGAA ATGGACAACC CCTTAATGGC TCACCTCATT
    TCACCAGAAC TCCAAAACAA GAAGGATATT TTGTTTGGGA ATATGGAAGA AATTTATCAC
    TTTCACAACA GGATATTCTT GAGAGAACTA GAAAATTATG TAGAATACCC AGAACTGGTA
    GGACGGTGCT TTCTGGATCA GATGGAAGAT TTCCAGATTT ATGAGAAATA CTGTCAAAAC
    AAACCTCGAT CTGAAAGTTT GTGGAGGCAA TTTTCAGATT CCATATTCTT TCAGGAATGT
    CAAAGGAAAC TCGATCACAA GCTCAGTTTG GACTCATACC TGCTGAAGCC AGTTCAAAGA
    ATAACCAAAT ACCAATTATT GCTAAAGGAG ATGCTGAAGT ACAGTAAGAA CTGTGAAGGT
    GCTGAAGATC TTCAGGAGGC ATTAAATTCT ATATTGGGTA TTCTCAAAGC AGTCAATGAT
    TCTATGCACC AGATAGCCAT CACAGGCTAT GATGGAAACC TGAATGAGCT GGGCAAGCTC
    CTAATGCAGG GATCCTTCAA CGTCTGGACT GATCATAAAA AGGGTCACTC AAAGGTGAAA
    GATTTGGCAC GCTTCAAGCC AATGCAGCGA CACCTGTTCC TCCACGAGAA AGCAGTGCTG
    TTCTGTAAAA AGCGAGAAGA AAATGGAGAA GGATATGAGA AGGCACCTTC TTATAGCTAT
    AAACACTCCT TAAATATGGC AGCAGTTGGA ATAACAGAAA ATGTCAAAGG TGATGCAAAA
    AAATTTGAAA TCTGGTATAA TGCAAGGGAA GAAGTTTACA TCATACAGGC ACCAACTCCT
    GAAGTTAAAG CCACTTGGGT AAATGAAATA AGAAAAGTGC TGACTAGTCA ACTGCAGGCA
    TGCAGAGAAG CCAGTCAACA TAGGACACTT GAACACACTC AGAGTTTACC TCTACCAGCA
    TCATCCTGTA CAAGTCCTTC AAGGAACAAC ATCAGAAACG TGAAAAAAAT GGATGAGAGA
    AAAGCTGATC TCACAAGTCT AGAAGGTTAT GTCACCACAG TAGCACCAAA GTACCCCGAG
    AAAGGCAAAG ATGACACTAA CTCTACCTCA GAATGCTCTG TACTGTCAAA AAAGCGCTTT
    ACGCTGCAGG GCTTTACTAA CCTCAAAAGT CAGAAAGCTT CTCCTACCAG TCCTGACAAA
    AAAGCTAAAC GGCACGAAGT AGTGAGTGAT CCAACTCCTT TTGGTGTAAG AGGTTGGGCT
    AAAACGTCTC ATTCTCTTGA TGCATCTGAA GAAAATGATG GCTGGTCCAG TGCTGAGGAG
    CCGCTGAATT CATCGGATGC AGAAGAAGAA GGAGGAGGAG GAGGAGAGAG AAATGAGATG
    AAGCTGACTC CTGGTAAATA TACAGTTGTG TCTGACTACG AGAAAGGAGT TTCTGAAGAA
    TTTGCAGTGA AAGGTGGAGA TCTAGTTCAA CTGATTCGTG AAGGGGAGGA GGGACTTTGG
    TATGTGAAGA ATCTGACCAC TGGTAGAGAA GGTTGGATCC CAGCAAACAA TCTGCTGATG
    CTCATAGGCA ATTCAAAATC AGCTCAATCT TTGAGCAGCT CTGAATCAGG CACTGCTTCC
    AGCACTTTGA GCACGTCATC AAGTTGCAGT GATAGCTGCA ATGCCAGCAG CACCAGCTTC
    TCAGACATTA AGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30674
    Clone ID Related Accession (Same CDS sequence) XM_015277848.1 , XM_015277848.2
    Accession Version XM_015277848.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACCTTAA GTCTGATCTC CTTGCTCTAC AAAGATATTG CTGAATTGTG GCTGTTTCTG 
    AAGAAATGCA CAGATGAAAT CATGCATCAG GATATCATCC CCCTCTATGC AGCAGATATT
    CATGACCAGT TAAAGAAACA ATTTGCTTAT CTGTCAGGTG GAAGAGGGGG AGATGGATGC
    CCTGTTATCA CTTTTCCCGA CTACCCAGCT TTCAGCGAGA TCCCTGAGAA GGAGTTCCAG
    AATGTCCTGA CCTACCTGAC CAGCATCCCG AGTTTACGAG ATGCTGGAAT CGGGTTTATT
    CTTGTCATAG ACCGCAGACA GGACAAATGG ACTTCTGTGA AAGCTTCCAT ACTGCGGATA
    GCAGCATCTT TCCCAGGGAA CTTGCAACTC GTCCTCGTTC TGCGCCCAAC AGGACTTATT
    CATCGAGCTC TCTCAGACAT TGCTTTCAAG TTCAACAGAG ATGAGTTTAA AATGAAAGTA
    CCTATCATAA TGCTGGGCTC AGTATCAGAA CTGCAGGGTT ACATTGATAA AACACAATTA
    ACAGAGGATC TTGGTGGCAC CCTGGATTAC TGCCATAACA GATGGCTATC CCTTCGCACT
    GCTATAGAAG GTTTTGCCCA AAAGGTTAAG CAAACAGCTC AGATTCTTCA GTCCTTTGGG
    ACTGAACTGG CTGAAACAGA ACTACCAAAT GATGTGCAGT CAACCAGCTC CCTTCTTGCA
    ATACATACGG AAAAGAAGGA CAAAATGAAG GAAGATATAA GGTTAGCAGT AGAACAGGGA
    GACGATATCC TTGGAAGTAT TACAGAGCCA GTTACTGAGA ACCCTGAATA CAAGCTGAAT
    CAAGACCAAC TAGACAATCA AACTACAGTA GAAAGGCTTT TGGCTCAGTT ACATGAAACA
    GAGTCCGCCT TTGATGAGTT TTGGATTAAG CACCAGCAAA AACTTGAGCA GTGTCTGCAC
    CTTCGGAATT TTGAACAGAA TTTTAGAGAG GTTAAAGCAG CTTTGGATAT TGTGTCCGAG
    AGACTGTCTG CTTTCACAGA TGTGGGAAAC AGCTGCTCAC ATGTGGAGCA CATTTTGAAA
    GATCTTGCCA ACTTTGAAGA AAAATCATGT GAAACTGTAG CAAAAGCCAG AATGCTAGCT
    TCAGAGGGTG ATGCTTTTAT TCAGAGCAAT CATTATGCTG TGGATTCCAT TATTCCAAAA
    TGCAGTGAGC TTCATCATCT ATGTGATGCC TTCACTACTG AGATGGAACA GAGGAGGAAT
    GTTCTTAATA AATCTCTAGA ACTGCATGGC TTACTGGAAA AGTCTATGAA GTGGTGCGAT
    GAAGGAATTT ATCTTCTGGC TTCACAACCT GTAGATAAGT GTCAGTCTCA GGATGGTGCA
    GAATCAGCAT TACAGGAGAT TGAGAAATTC CTAGACACTG GTGCAGGCAA TAAAATTAAG
    GAGTTGAATA AAGTTTACAG AGAGTATGCA CACATCCTCA ATGAGGACCT AAAGGAGCAT
    ATACAAAAGG TTTTCCAGAA GCAAGAAAGC ATGGAAGAAA TGTTTCAAAA GAGACAAGTA
    AGTCTTAAGA AACTGGCAGC TAAGCAAACA CGTCCTGTTC AGCCAGTTGC ACCTAGACCC
    GAAGCCTTTG TAAAATCTCC TTGTACCTCT CCAGGTCTTC AAAGAGATTG TGTATCCAAC
    ACAGAAAGCG GTGTGCTTCG GAAAGTAAAC TACAGAAGAG GAAAGAGTGA AATGAGTGAA
    CTCCATCAAA GCAGAGGAGG ATCTACAATG GATGATGAAG AAAACCTAGC TGTGTTACGG
    CAGCATGTAA TGAATGAACT TATTGAAACG GAACGAGCAT ATGTGGAAGA ACTTCTCTGT
    GTTCTTGAGG GGTATGCTGC AGAAATGGAC AACCCCTTAA TGGCTCACCT CATTTCACCA
    GAACTCCAAA ACAAGAAGGA TATTTTGTTT GGGAATATGG AAGAAATTTA TCACTTTCAC
    AACAGGATAT TCTTGAGAGA ACTAGAAAAT TATGTAGAAT ACCCAGAACT GGTAGGACGG
    TGCTTTCTGG ATCAGATGGA AGATTTCCAG ATTTATGAGA AATACTGTCA AAACAAACCT
    CGATCTGAAA GTTTGTGGAG GCAATTTTCA GATTCCATAT TCTTTCAGGA ATGTCAAAGG
    AAACTCGATC ACAAGCTCAG TTTGGACTCA TACCTGCTGA AGCCAGTTCA AAGAATAACC
    AAATACCAAT TATTGCTAAA GGAGATGCTG AAGTACAGTA AGAACTGTGA AGGTGCTGAA
    GATCTTCAGG AGGCATTAAA TTCTATATTG GGTATTCTCA AAGCAGTCAA TGATTCTATG
    CACCAGATAG CCATCACAGG CTATGATGGA AACCTGAATG AGCTGGGCAA GCTCCTAATG
    CAGGGATCCT TCAACGTCTG GACTGATCAT AAAAAGGGTC ACTCAAAGGT GAAAGATTTG
    GCACGCTTCA AGCCAATGCA GCGACACCTG TTCCTCCACG AGAAAGCAGT GCTGTTCTGT
    AAAAAGCGAG AAGAAAATGG AGAAGGATAT GAGAAGGCAC CTTCTTATAG CTATAAACAC
    TCCTTAAATA TGGCAGCAGT TGGAATAACA GAAAATGTCA AAGGTGATGC AAAAAAATTT
    GAAATCTGGT ATAATGCAAG GGAAGAAGTT TACATCATAC AGGCACCAAC TCCTGAAGTT
    AAAGCCACTT GGGTAAATGA AATAAGAAAA GTGCTGACTA GTCAACTGCA GGCATGCAGA
    GAAGCCAGTC AACATAGGAC ACTTGAACAC ACTCAGAGTT TACCTCTACC AGCATCATCC
    TGTACAAGTC CTTCAAGGAA CAACATCAGA AACGTGAAAA AAATGGATGA GAGAAAAGCT
    GATCTCACAA GTCTAGAAGG TTATGTCACC ACAGTAGCAC CAAAGTACCC CGAGAAAGGC
    AAAGATGACA CTAACTCTAC CTCAGAATGC TCTGTACTGT CAAAAAAGCG CTTTACGCTG
    CAGGGCTTTA CTAACCTCAA AAGTCAGAAA GCTTCTCCTA CCAGTCCTGA CAAAAAAGCT
    AAACGGCACG AAGTAGTGAG TGATCCAACT CCTTTTGGTG TAAGAGGTTG GGCTAAAACG
    TCTCATTCTC TTGATGCATC TGAAGAAAAT GATGGCTGGT CCAGTGCTGA GGAGCCGCTG
    AATTCATCGG ATGCAGAAGA AGAAGGAGGA GGAGGAGGAG AGAGAAATGA GATGAAGCTG
    ACTCCTGGTA AATATACAGT TGTGTCTGAC TACGAGAAAG GAGTTTCTGA AGAATTTGCA
    GTGAAAGGTG GAGATCTAGT TCAACTGATT CGTGAAGGGG AGGAGGGACT TTGGTATGTG
    AAGAATCTGA CCACTGGTAG AGAAGGTTGG ATCCCAGCAA ACAATCTGCT GATGCTCATA
    GGCAATTCAA AATCAGCTCA ATCTTTGAGC AGCTCTGAAT CAGGCACTGC TTCCAGCACT
    TTGAGCACGT CATCAAGTTG CAGTGATAGC TGCAATGCCA GCAGCACCAG CTTCTCAGAC
    ATTAAGGGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133334.1
    CDS236..3787
    Translation

    Target ORF information:

    RefSeq Version XM_015277848.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACCTTAA GTCTGATCTC CTTGCTCTAC AAAGATATTG CTGAATTGTG GCTGTTTCTG 
    AAGAAATGCA CAGATGAAAT CATGCATCAG GATATCATCC CCCTCTATGC AGCAGATATT
    CATGACCAGT TAAAGAAACA ATTTGCTTAT CTGTCAGGTG GAAGAGGGGG AGATGGATGC
    CCTGTTATCA CTTTTCCCGA CTACCCAGCT TTCAGCGAGA TCCCTGAGAA GGAGTTCCAG
    AATGTCCTGA CCTACCTGAC CAGCATCCCG AGTTTACGAG ATGCTGGAAT CGGGTTTATT
    CTTGTCATAG ACCGCAGACA GGACAAATGG ACTTCTGTGA AAGCTTCCAT ACTGCGGATA
    GCAGCATCTT TCCCAGGGAA CTTGCAACTC GTCCTCGTTC TGCGCCCAAC AGGACTTATT
    CATCGAGCTC TCTCAGACAT TGCTTTCAAG TTCAACAGAG ATGAGTTTAA AATGAAAGTA
    CCTATCATAA TGCTGGGCTC AGTATCAGAA CTGCAGGGTT ACATTGATAA AACACAATTA
    ACAGAGGATC TTGGTGGCAC CCTGGATTAC TGCCATAACA GATGGCTATC CCTTCGCACT
    GCTATAGAAG GTTTTGCCCA AAAGGTTAAG CAAACAGCTC AGATTCTTCA GTCCTTTGGG
    ACTGAACTGG CTGAAACAGA ACTACCAAAT GATGTGCAGT CAACCAGCTC CCTTCTTGCA
    ATACATACGG AAAAGAAGGA CAAAATGAAG GAAGATATAA GGTTAGCAGT AGAACAGGGA
    GACGATATCC TTGGAAGTAT TACAGAGCCA GTTACTGAGA ACCCTGAATA CAAGCTGAAT
    CAAGACCAAC TAGACAATCA AACTACAGTA GAAAGGCTTT TGGCTCAGTT ACATGAAACA
    GAGTCCGCCT TTGATGAGTT TTGGATTAAG CACCAGCAAA AACTTGAGCA GTGTCTGCAC
    CTTCGGAATT TTGAACAGAA TTTTAGAGAG GTTAAAGCAG CTTTGGATAT TGTGTCCGAG
    AGACTGTCTG CTTTCACAGA TGTGGGAAAC AGCTGCTCAC ATGTGGAGCA CATTTTGAAA
    GATCTTGCCA ACTTTGAAGA AAAATCATGT GAAACTGTAG CAAAAGCCAG AATGCTAGCT
    TCAGAGGGTG ATGCTTTTAT TCAGAGCAAT CATTATGCTG TGGATTCCAT TATTCCAAAA
    TGCAGTGAGC TTCATCATCT ATGTGATGCC TTCACTACTG AGATGGAACA GAGGAGGAAT
    GTTCTTAATA AATCTCTAGA ACTGCATGGC TTACTGGAAA AGTCTATGAA GTGGTGCGAT
    GAAGGAATTT ATCTTCTGGC TTCACAACCT GTAGATAAGT GTCAGTCTCA GGATGGTGCA
    GAATCAGCAT TACAGGAGAT TGAGAAATTC CTAGACACTG GTGCAGGCAA TAAAATTAAG
    GAGTTGAATA AAGTTTACAG AGAGTATGCA CACATCCTCA ATGAGGACCT AAAGGAGCAT
    ATACAAAAGG TTTTCCAGAA GCAAGAAAGC ATGGAAGAAA TGTTTCAAAA GAGACAAGTA
    AGTCTTAAGA AACTGGCAGC TAAGCAAACA CGTCCTGTTC AGCCAGTTGC ACCTAGACCC
    GAAGCCTTTG TAAAATCTCC TTGTACCTCT CCAGGTCTTC AAAGAGATTG TGTATCCAAC
    ACAGAAAGCG GTGTGCTTCG GAAAGTAAAC TACAGAAGAG GAAAGAGTGA AATGAGTGAA
    CTCCATCAAA GCAGAGGAGG ATCTACAATG GATGATGAAG AAAACCTAGC TGTGTTACGG
    CAGCATGTAA TGAATGAACT TATTGAAACG GAACGAGCAT ATGTGGAAGA ACTTCTCTGT
    GTTCTTGAGG GGTATGCTGC AGAAATGGAC AACCCCTTAA TGGCTCACCT CATTTCACCA
    GAACTCCAAA ACAAGAAGGA TATTTTGTTT GGGAATATGG AAGAAATTTA TCACTTTCAC
    AACAGGATAT TCTTGAGAGA ACTAGAAAAT TATGTAGAAT ACCCAGAACT GGTAGGACGG
    TGCTTTCTGG ATCAGATGGA AGATTTCCAG ATTTATGAGA AATACTGTCA AAACAAACCT
    CGATCTGAAA GTTTGTGGAG GCAATTTTCA GATTCCATAT TCTTTCAGGA ATGTCAAAGG
    AAACTCGATC ACAAGCTCAG TTTGGACTCA TACCTGCTGA AGCCAGTTCA AAGAATAACC
    AAATACCAAT TATTGCTAAA GGAGATGCTG AAGTACAGTA AGAACTGTGA AGGTGCTGAA
    GATCTTCAGG AGGCATTAAA TTCTATATTG GGTATTCTCA AAGCAGTCAA TGATTCTATG
    CACCAGATAG CCATCACAGG CTATGATGGA AACCTGAATG AGCTGGGCAA GCTCCTAATG
    CAGGGATCCT TCAACGTCTG GACTGATCAT AAAAAGGGTC ACTCAAAGGT GAAAGATTTG
    GCACGCTTCA AGCCAATGCA GCGACACCTG TTCCTCCACG AGAAAGCAGT GCTGTTCTGT
    AAAAAGCGAG AAGAAAATGG AGAAGGATAT GAGAAGGCAC CTTCTTATAG CTATAAACAC
    TCCTTAAATA TGGCAGCAGT TGGAATAACA GAAAATGTCA AAGGTGATGC AAAAAAATTT
    GAAATCTGGT ATAATGCAAG GGAAGAAGTT TACATCATAC AGGCACCAAC TCCTGAAGTT
    AAAGCCACTT GGGTAAATGA AATAAGAAAA GTGCTGACTA GTCAACTGCA GGCATGCAGA
    GAAGCCAGTC AACATAGGAC ACTTGAACAC ACTCAGAGTT TACCTCTACC AGCATCATCC
    TGTACAAGTC CTTCAAGGAA CAACATCAGA AACGTGAAAA AAATGGATGA GAGAAAAGCT
    GATCTCACAA GTCTAGAAGG TTATGTCACC ACAGTAGCAC CAAAGTACCC CGAGAAAGGC
    AAAGATGACA CTAACTCTAC CTCAGAATGC TCTGTACTGT CAAAAAAGCG CTTTACGCTG
    CAGGGCTTTA CTAACCTCAA AAGTCAGAAA GCTTCTCCTA CCAGTCCTGA CAAAAAAGCT
    AAACGGCACG AAGTAGTGAG TGATCCAACT CCTTTTGGTG TAAGAGGTTG GGCTAAAACG
    TCTCATTCTC TTGATGCATC TGAAGAAAAT GATGGCTGGT CCAGTGCTGA GGAGCCGCTG
    AATTCATCGG ATGCAGAAGA AGAAGGAGGA GGAGGAGGAG AGAGAAATGA GATGAAGCTG
    ACTCCTGGTA AATATACAGT TGTGTCTGAC TACGAGAAAG GAGTTTCTGA AGAATTTGCA
    GTGAAAGGTG GAGATCTAGT TCAACTGATT CGTGAAGGGG AGGAGGGACT TTGGTATGTG
    AAGAATCTGA CCACTGGTAG AGAAGGTTGG ATCCCAGCAA ACAATCTGCT GATGCTCATA
    GGCAATTCAA AATCAGCTCA ATCTTTGAGC AGCTCTGAAT CAGGCACTGC TTCCAGCACT
    TTGAGCACGT CATCAAGTTG CAGTGATAGC TGCAATGCCA GCAGCACCAG CTTCTCAGAC
    ATTAAGGGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30674
    Clone ID Related Accession (Same CDS sequence) XM_015277848.1 , XM_015277848.2
    Accession Version XM_015277848.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277848.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACCTTAA GTCTGATCTC CTTGCTCTAC AAAGATATTG CTGAATTGTG GCTGTTTCTG 
    AAGAAATGCA CAGATGAAAT CATGCATCAG GATATCATCC CCCTCTATGC AGCAGATATT
    CATGACCAGT TAAAGAAACA ATTTGCTTAT CTGTCAGGTG GAAGAGGGGG AGATGGATGC
    CCTGTTATCA CTTTTCCCGA CTACCCAGCT TTCAGCGAGA TCCCTGAGAA GGAGTTCCAG
    AATGTCCTGA CCTACCTGAC CAGCATCCCG AGTTTACGAG ATGCTGGAAT CGGGTTTATT
    CTTGTCATAG ACCGCAGACA GGACAAATGG ACTTCTGTGA AAGCTTCCAT ACTGCGGATA
    GCAGCATCTT TCCCAGGGAA CTTGCAACTC GTCCTCGTTC TGCGCCCAAC AGGACTTATT
    CATCGAGCTC TCTCAGACAT TGCTTTCAAG TTCAACAGAG ATGAGTTTAA AATGAAAGTA
    CCTATCATAA TGCTGGGCTC AGTATCAGAA CTGCAGGGTT ACATTGATAA AACACAATTA
    ACAGAGGATC TTGGTGGCAC CCTGGATTAC TGCCATAACA GATGGCTATC CCTTCGCACT
    GCTATAGAAG GTTTTGCCCA AAAGGTTAAG CAAACAGCTC AGATTCTTCA GTCCTTTGGG
    ACTGAACTGG CTGAAACAGA ACTACCAAAT GATGTGCAGT CAACCAGCTC CCTTCTTGCA
    ATACATACGG AAAAGAAGGA CAAAATGAAG GAAGATATAA GGTTAGCAGT AGAACAGGGA
    GACGATATCC TTGGAAGTAT TACAGAGCCA GTTACTGAGA ACCCTGAATA CAAGCTGAAT
    CAAGACCAAC TAGACAATCA AACTACAGTA GAAAGGCTTT TGGCTCAGTT ACATGAAACA
    GAGTCCGCCT TTGATGAGTT TTGGATTAAG CACCAGCAAA AACTTGAGCA GTGTCTGCAC
    CTTCGGAATT TTGAACAGAA TTTTAGAGAG GTTAAAGCAG CTTTGGATAT TGTGTCCGAG
    AGACTGTCTG CTTTCACAGA TGTGGGAAAC AGCTGCTCAC ATGTGGAGCA CATTTTGAAA
    GATCTTGCCA ACTTTGAAGA AAAATCATGT GAAACTGTAG CAAAAGCCAG AATGCTAGCT
    TCAGAGGGTG ATGCTTTTAT TCAGAGCAAT CATTATGCTG TGGATTCCAT TATTCCAAAA
    TGCAGTGAGC TTCATCATCT ATGTGATGCC TTCACTACTG AGATGGAACA GAGGAGGAAT
    GTTCTTAATA AATCTCTAGA ACTGCATGGC TTACTGGAAA AGTCTATGAA GTGGTGCGAT
    GAAGGAATTT ATCTTCTGGC TTCACAACCT GTAGATAAGT GTCAGTCTCA GGATGGTGCA
    GAATCAGCAT TACAGGAGAT TGAGAAATTC CTAGACACTG GTGCAGGCAA TAAAATTAAG
    GAGTTGAATA AAGTTTACAG AGAGTATGCA CACATCCTCA ATGAGGACCT AAAGGAGCAT
    ATACAAAAGG TTTTCCAGAA GCAAGAAAGC ATGGAAGAAA TGTTTCAAAA GAGACAAGTA
    AGTCTTAAGA AACTGGCAGC TAAGCAAACA CGTCCTGTTC AGCCAGTTGC ACCTAGACCC
    GAAGCCTTTG TAAAATCTCC TTGTACCTCT CCAGGTCTTC AAAGAGATTG TGTATCCAAC
    ACAGAAAGCG GTGTGCTTCG GAAAGTAAAC TACAGAAGAG GAAAGAGTGA AATGAGTGAA
    CTCCATCAAA GCAGAGGAGG ATCTACAATG GATGATGAAG AAAACCTAGC TGTGTTACGG
    CAGCATGTAA TGAATGAACT TATTGAAACG GAACGAGCAT ATGTGGAAGA ACTTCTCTGT
    GTTCTTGAGG GGTATGCTGC AGAAATGGAC AACCCCTTAA TGGCTCACCT CATTTCACCA
    GAACTCCAAA ACAAGAAGGA TATTTTGTTT GGGAATATGG AAGAAATTTA TCACTTTCAC
    AACAGGATAT TCTTGAGAGA ACTAGAAAAT TATGTAGAAT ACCCAGAACT GGTAGGACGG
    TGCTTTCTGG ATCAGATGGA AGATTTCCAG ATTTATGAGA AATACTGTCA AAACAAACCT
    CGATCTGAAA GTTTGTGGAG GCAATTTTCA GATTCCATAT TCTTTCAGGA ATGTCAAAGG
    AAACTCGATC ACAAGCTCAG TTTGGACTCA TACCTGCTGA AGCCAGTTCA AAGAATAACC
    AAATACCAAT TATTGCTAAA GGAGATGCTG AAGTACAGTA AGAACTGTGA AGGTGCTGAA
    GATCTTCAGG AGGCATTAAA TTCTATATTG GGTATTCTCA AAGCAGTCAA TGATTCTATG
    CACCAGATAG CCATCACAGG CTATGATGGA AACCTGAATG AGCTGGGCAA GCTCCTAATG
    CAGGGATCCT TCAACGTCTG GACTGATCAT AAAAAGGGTC ACTCAAAGGT GAAAGATTTG
    GCACGCTTCA AGCCAATGCA GCGACACCTG TTCCTCCACG AGAAAGCAGT GCTGTTCTGT
    AAAAAGCGAG AAGAAAATGG AGAAGGATAT GAGAAGGCAC CTTCTTATAG CTATAAACAC
    TCCTTAAATA TGGCAGCAGT TGGAATAACA GAAAATGTCA AAGGTGATGC AAAAAAATTT
    GAAATCTGGT ATAATGCAAG GGAAGAAGTT TACATCATAC AGGCACCAAC TCCTGAAGTT
    AAAGCCACTT GGGTAAATGA AATAAGAAAA GTGCTGACTA GTCAACTGCA GGCATGCAGA
    GAAGCCAGTC AACATAGGAC ACTTGAACAC ACTCAGAGTT TACCTCTACC AGCATCATCC
    TGTACAAGTC CTTCAAGGAA CAACATCAGA AACGTGAAAA AAATGGATGA GAGAAAAGCT
    GATCTCACAA GTCTAGAAGG TTATGTCACC ACAGTAGCAC CAAAGTACCC CGAGAAAGGC
    AAAGATGACA CTAACTCTAC CTCAGAATGC TCTGTACTGT CAAAAAAGCG CTTTACGCTG
    CAGGGCTTTA CTAACCTCAA AAGTCAGAAA GCTTCTCCTA CCAGTCCTGA CAAAAAAGCT
    AAACGGCACG AAGTAGTGAG TGATCCAACT CCTTTTGGTG TAAGAGGTTG GGCTAAAACG
    TCTCATTCTC TTGATGCATC TGAAGAAAAT GATGGCTGGT CCAGTGCTGA GGAGCCGCTG
    AATTCATCGG ATGCAGAAGA AGAAGGAGGA GGAGGAGGAG AGAGAAATGA GATGAAGCTG
    ACTCCTGGTA AATATACAGT TGTGTCTGAC TACGAGAAAG GAGTTTCTGA AGAATTTGCA
    GTGAAAGGTG GAGATCTAGT TCAACTGATT CGTGAAGGGG AGGAGGGACT TTGGTATGTG
    AAGAATCTGA CCACTGGTAG AGAAGGTTGG ATCCCAGCAA ACAATCTGCT GATGCTCATA
    GGCAATTCAA AATCAGCTCA ATCTTTGAGC AGCTCTGAAT CAGGCACTGC TTCCAGCACT
    TTGAGCACGT CATCAAGTTG CAGTGATAGC TGCAATGCCA GCAGCACCAG CTTCTCAGAC
    ATTAAGGGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133334.1
    CDS224..3775
    Translation

    Target ORF information:

    RefSeq Version XM_015277848.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277848.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACCTTAA GTCTGATCTC CTTGCTCTAC AAAGATATTG CTGAATTGTG GCTGTTTCTG 
    AAGAAATGCA CAGATGAAAT CATGCATCAG GATATCATCC CCCTCTATGC AGCAGATATT
    CATGACCAGT TAAAGAAACA ATTTGCTTAT CTGTCAGGTG GAAGAGGGGG AGATGGATGC
    CCTGTTATCA CTTTTCCCGA CTACCCAGCT TTCAGCGAGA TCCCTGAGAA GGAGTTCCAG
    AATGTCCTGA CCTACCTGAC CAGCATCCCG AGTTTACGAG ATGCTGGAAT CGGGTTTATT
    CTTGTCATAG ACCGCAGACA GGACAAATGG ACTTCTGTGA AAGCTTCCAT ACTGCGGATA
    GCAGCATCTT TCCCAGGGAA CTTGCAACTC GTCCTCGTTC TGCGCCCAAC AGGACTTATT
    CATCGAGCTC TCTCAGACAT TGCTTTCAAG TTCAACAGAG ATGAGTTTAA AATGAAAGTA
    CCTATCATAA TGCTGGGCTC AGTATCAGAA CTGCAGGGTT ACATTGATAA AACACAATTA
    ACAGAGGATC TTGGTGGCAC CCTGGATTAC TGCCATAACA GATGGCTATC CCTTCGCACT
    GCTATAGAAG GTTTTGCCCA AAAGGTTAAG CAAACAGCTC AGATTCTTCA GTCCTTTGGG
    ACTGAACTGG CTGAAACAGA ACTACCAAAT GATGTGCAGT CAACCAGCTC CCTTCTTGCA
    ATACATACGG AAAAGAAGGA CAAAATGAAG GAAGATATAA GGTTAGCAGT AGAACAGGGA
    GACGATATCC TTGGAAGTAT TACAGAGCCA GTTACTGAGA ACCCTGAATA CAAGCTGAAT
    CAAGACCAAC TAGACAATCA AACTACAGTA GAAAGGCTTT TGGCTCAGTT ACATGAAACA
    GAGTCCGCCT TTGATGAGTT TTGGATTAAG CACCAGCAAA AACTTGAGCA GTGTCTGCAC
    CTTCGGAATT TTGAACAGAA TTTTAGAGAG GTTAAAGCAG CTTTGGATAT TGTGTCCGAG
    AGACTGTCTG CTTTCACAGA TGTGGGAAAC AGCTGCTCAC ATGTGGAGCA CATTTTGAAA
    GATCTTGCCA ACTTTGAAGA AAAATCATGT GAAACTGTAG CAAAAGCCAG AATGCTAGCT
    TCAGAGGGTG ATGCTTTTAT TCAGAGCAAT CATTATGCTG TGGATTCCAT TATTCCAAAA
    TGCAGTGAGC TTCATCATCT ATGTGATGCC TTCACTACTG AGATGGAACA GAGGAGGAAT
    GTTCTTAATA AATCTCTAGA ACTGCATGGC TTACTGGAAA AGTCTATGAA GTGGTGCGAT
    GAAGGAATTT ATCTTCTGGC TTCACAACCT GTAGATAAGT GTCAGTCTCA GGATGGTGCA
    GAATCAGCAT TACAGGAGAT TGAGAAATTC CTAGACACTG GTGCAGGCAA TAAAATTAAG
    GAGTTGAATA AAGTTTACAG AGAGTATGCA CACATCCTCA ATGAGGACCT AAAGGAGCAT
    ATACAAAAGG TTTTCCAGAA GCAAGAAAGC ATGGAAGAAA TGTTTCAAAA GAGACAAGTA
    AGTCTTAAGA AACTGGCAGC TAAGCAAACA CGTCCTGTTC AGCCAGTTGC ACCTAGACCC
    GAAGCCTTTG TAAAATCTCC TTGTACCTCT CCAGGTCTTC AAAGAGATTG TGTATCCAAC
    ACAGAAAGCG GTGTGCTTCG GAAAGTAAAC TACAGAAGAG GAAAGAGTGA AATGAGTGAA
    CTCCATCAAA GCAGAGGAGG ATCTACAATG GATGATGAAG AAAACCTAGC TGTGTTACGG
    CAGCATGTAA TGAATGAACT TATTGAAACG GAACGAGCAT ATGTGGAAGA ACTTCTCTGT
    GTTCTTGAGG GGTATGCTGC AGAAATGGAC AACCCCTTAA TGGCTCACCT CATTTCACCA
    GAACTCCAAA ACAAGAAGGA TATTTTGTTT GGGAATATGG AAGAAATTTA TCACTTTCAC
    AACAGGATAT TCTTGAGAGA ACTAGAAAAT TATGTAGAAT ACCCAGAACT GGTAGGACGG
    TGCTTTCTGG ATCAGATGGA AGATTTCCAG ATTTATGAGA AATACTGTCA AAACAAACCT
    CGATCTGAAA GTTTGTGGAG GCAATTTTCA GATTCCATAT TCTTTCAGGA ATGTCAAAGG
    AAACTCGATC ACAAGCTCAG TTTGGACTCA TACCTGCTGA AGCCAGTTCA AAGAATAACC
    AAATACCAAT TATTGCTAAA GGAGATGCTG AAGTACAGTA AGAACTGTGA AGGTGCTGAA
    GATCTTCAGG AGGCATTAAA TTCTATATTG GGTATTCTCA AAGCAGTCAA TGATTCTATG
    CACCAGATAG CCATCACAGG CTATGATGGA AACCTGAATG AGCTGGGCAA GCTCCTAATG
    CAGGGATCCT TCAACGTCTG GACTGATCAT AAAAAGGGTC ACTCAAAGGT GAAAGATTTG
    GCACGCTTCA AGCCAATGCA GCGACACCTG TTCCTCCACG AGAAAGCAGT GCTGTTCTGT
    AAAAAGCGAG AAGAAAATGG AGAAGGATAT GAGAAGGCAC CTTCTTATAG CTATAAACAC
    TCCTTAAATA TGGCAGCAGT TGGAATAACA GAAAATGTCA AAGGTGATGC AAAAAAATTT
    GAAATCTGGT ATAATGCAAG GGAAGAAGTT TACATCATAC AGGCACCAAC TCCTGAAGTT
    AAAGCCACTT GGGTAAATGA AATAAGAAAA GTGCTGACTA GTCAACTGCA GGCATGCAGA
    GAAGCCAGTC AACATAGGAC ACTTGAACAC ACTCAGAGTT TACCTCTACC AGCATCATCC
    TGTACAAGTC CTTCAAGGAA CAACATCAGA AACGTGAAAA AAATGGATGA GAGAAAAGCT
    GATCTCACAA GTCTAGAAGG TTATGTCACC ACAGTAGCAC CAAAGTACCC CGAGAAAGGC
    AAAGATGACA CTAACTCTAC CTCAGAATGC TCTGTACTGT CAAAAAAGCG CTTTACGCTG
    CAGGGCTTTA CTAACCTCAA AAGTCAGAAA GCTTCTCCTA CCAGTCCTGA CAAAAAAGCT
    AAACGGCACG AAGTAGTGAG TGATCCAACT CCTTTTGGTG TAAGAGGTTG GGCTAAAACG
    TCTCATTCTC TTGATGCATC TGAAGAAAAT GATGGCTGGT CCAGTGCTGA GGAGCCGCTG
    AATTCATCGG ATGCAGAAGA AGAAGGAGGA GGAGGAGGAG AGAGAAATGA GATGAAGCTG
    ACTCCTGGTA AATATACAGT TGTGTCTGAC TACGAGAAAG GAGTTTCTGA AGAATTTGCA
    GTGAAAGGTG GAGATCTAGT TCAACTGATT CGTGAAGGGG AGGAGGGACT TTGGTATGTG
    AAGAATCTGA CCACTGGTAG AGAAGGTTGG ATCCCAGCAA ACAATCTGCT GATGCTCATA
    GGCAATTCAA AATCAGCTCA ATCTTTGAGC AGCTCTGAAT CAGGCACTGC TTCCAGCACT
    TTGAGCACGT CATCAAGTTG CAGTGATAGC TGCAATGCCA GCAGCACCAG CTTCTCAGAC
    ATTAAGGGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30675
    Clone ID Related Accession (Same CDS sequence) XM_015277849.1 , XM_015277849.2
    Accession Version XM_015277849.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCAAATT TAAATGAAAC AGATGAAATC ATGCATCAGG ATATCATCCC CCTCTATGCA 
    GCAGATATTC ATGACCAGTT AAAGAAACAA TTTGCTTATC TGTCAGGTGG AAGAGGGGGA
    GATGGATGCC CTGTTATCAC TTTTCCCGAC TACCCAGCTT TCAGCGAGAT CCCTGAGAAG
    GAGTTCCAGA ATGTCCTGAC CTACCTGACC AGCATCCCGA GTTTACGAGA TGCTGGAATC
    GGGTTTATTC TTGTCATAGA CCGCAGACAG GACAAATGGA CTTCTGTGAA AGCTTCCATA
    CTGCGGATAG CAGCATCTTT CCCAGGGAAC TTGCAACTCG TCCTCGTTCT GCGCCCAACA
    GGACTTATTC ATCGAGCTCT CTCAGACATT GCTTTCAAGT TCAACAGAGA TGAGTTTAAA
    ATGAAAGTAC CTATCATAAT GCTGGGCTCA GTATCAGAAC TGCAGGGTTA CATTGATAAA
    ACACAATTAA CAGAGGATCT TGGTGGCACC CTGGATTACT GCCATAACAG ATGGCTATCC
    CTTCGCACTG CTATAGAAGG TTTTGCCCAA AAGGTTAAGC AAACAGCTCA GATTCTTCAG
    TCCTTTGGGA CTGAACTGGC TGAAACAGAA CTACCAAATG ATGTGCAGTC AACCAGCTCC
    CTTCTTGCAA TACATACGGA AAAGAAGGAC AAAATGAAGG AAGATATAAG GTTAGCAGTA
    GAACAGGGAG ACGATATCCT TGGAAGTATT ACAGAGCCAG TTACTGAGAA CCCTGAATAC
    AAGCTGAATC AAGACCAACT AGACAATCAA ACTACAGTAG AAAGGCTTTT GGCTCAGTTA
    CATGAAACAG AGTCCGCCTT TGATGAGTTT TGGATTAAGC ACCAGCAAAA ACTTGAGCAG
    TGTCTGCACC TTCGGAATTT TGAACAGAAT TTTAGAGAGG TTAAAGCAGC TTTGGATATT
    GTGTCCGAGA GACTGTCTGC TTTCACAGAT GTGGGAAACA GCTGCTCACA TGTGGAGCAC
    ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA AAATCATGTG AAACTGTAGC AAAAGCCAGA
    ATGCTAGCTT CAGAGGGTGA TGCTTTTATT CAGAGCAATC ATTATGCTGT GGATTCCATT
    ATTCCAAAAT GCAGTGAGCT TCATCATCTA TGTGATGCCT TCACTACTGA GATGGAACAG
    AGGAGGAATG TTCTTAATAA ATCTCTAGAA CTGCATGGCT TACTGGAAAA GTCTATGAAG
    TGGTGCGATG AAGGAATTTA TCTTCTGGCT TCACAACCTG TAGATAAGTG TCAGTCTCAG
    GATGGTGCAG AATCAGCATT ACAGGAGATT GAGAAATTCC TAGACACTGG TGCAGGCAAT
    AAAATTAAGG AGTTGAATAA AGTTTACAGA GAGTATGCAC ACATCCTCAA TGAGGACCTA
    AAGGAGCATA TACAAAAGGT TTTCCAGAAG CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG
    AGACAAGTAA GTCTTAAGAA ACTGGCAGCT AAGCAAACAC GTCCTGTTCA GCCAGTTGCA
    CCTAGACCCG AAGCCTTTGT AAAATCTCCT TGTACCTCTC CAGGTCTTCA AAGAGATTGT
    GTATCCAACA CAGAAAGCGG TGTGCTTCGG AAAGTAAACT ACAGAAGAGG AAAGAGTGAA
    ATGAGTGAAC TCCATCAAAG CAGAGGAGGA TCTACAATGG ATGATGAAGA AAACCTAGCT
    GTGTTACGGC AGCATGTAAT GAATGAACTT ATTGAAACGG AACGAGCATA TGTGGAAGAA
    CTTCTCTGTG TTCTTGAGGG GTATGCTGCA GAAATGGACA ACCCCTTAAT GGCTCACCTC
    ATTTCACCAG AACTCCAAAA CAAGAAGGAT ATTTTGTTTG GGAATATGGA AGAAATTTAT
    CACTTTCACA ACAGGATATT CTTGAGAGAA CTAGAAAATT ATGTAGAATA CCCAGAACTG
    GTAGGACGGT GCTTTCTGGA TCAGATGGAA GATTTCCAGA TTTATGAGAA ATACTGTCAA
    AACAAACCTC GATCTGAAAG TTTGTGGAGG CAATTTTCAG ATTCCATATT CTTTCAGGAA
    TGTCAAAGGA AACTCGATCA CAAGCTCAGT TTGGACTCAT ACCTGCTGAA GCCAGTTCAA
    AGAATAACCA AATACCAATT ATTGCTAAAG GAGATGCTGA AGTACAGTAA GAACTGTGAA
    GGTGCTGAAG ATCTTCAGGA GGCATTAAAT TCTATATTGG GTATTCTCAA AGCAGTCAAT
    GATTCTATGC ACCAGATAGC CATCACAGGC TATGATGGAA ACCTGAATGA GCTGGGCAAG
    CTCCTAATGC AGGGATCCTT CAACGTCTGG ACTGATCATA AAAAGGGTCA CTCAAAGGTG
    AAAGATTTGG CACGCTTCAA GCCAATGCAG CGACACCTGT TCCTCCACGA GAAAGCAGTG
    CTGTTCTGTA AAAAGCGAGA AGAAAATGGA GAAGGATATG AGAAGGCACC TTCTTATAGC
    TATAAACACT CCTTAAATAT GGCAGCAGTT GGAATAACAG AAAATGTCAA AGGTGATGCA
    AAAAAATTTG AAATCTGGTA TAATGCAAGG GAAGAAGTTT ACATCATACA GGCACCAACT
    CCTGAAGTTA AAGCCACTTG GGTAAATGAA ATAAGAAAAG TGCTGACTAG TCAACTGCAG
    GCATGCAGAG AAGCCAGTCA ACATAGGACA CTTGAACACA CTCAGAGTTT ACCTCTACCA
    GCATCATCCT GTACAAGTCC TTCAAGGAAC AACATCAGAA ACGTGAAAAA AATGGATGAG
    AGAAAAGCTG ATCTCACAAG TCTAGAAGGT TATGTCACCA CAGTAGCACC AAAGTACCCC
    GAGAAAGGCA AAGATGACAC TAACTCTACC TCAGAATGCT CTGTACTGTC AAAAAAGCGC
    TTTACGCTGC AGGGCTTTAC TAACCTCAAA AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC
    AAAAAAGCTA AACGGCACGA AGTAGTGAGT GATCCAACTC CTTTTGGTGT AAGAGGTTGG
    GCTAAAACGT CTCATTCTCT TGATGCATCT GAAGAAAATG ATGGCTGGTC CAGTGCTGAG
    GAGCCGCTGA ATTCATCGGA TGCAGAAGAA GAAGGAGGAG GAGGAGGAGA GAGAAATGAG
    ATGAAGCTGA CTCCTGGTAA ATATACAGTT GTGTCTGACT ACGAGAAAGG AGTTTCTGAA
    GAATTTGCAG TGAAAGGTGG AGATCTAGTT CAACTGATTC GTGAAGGGGA GGAGGGACTT
    TGGTATGTGA AGAATCTGAC CACTGGTAGA GAAGGTTGGA TCCCAGCAAA CAATCTGCTG
    ATGCTCATAG GCAATTCAAA ATCAGCTCAA TCTTTGAGCA GCTCTGAATC AGGCACTGCT
    TCCAGCACTT TGAGCACGTC ATCAAGTTGC AGTGATAGCT GCAATGCCAG CAGCACCAGC
    TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133335.1
    CDS84..3584
    Translation

    Target ORF information:

    RefSeq Version XM_015277849.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277849.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCAAATT TAAATGAAAC AGATGAAATC ATGCATCAGG ATATCATCCC CCTCTATGCA 
    GCAGATATTC ATGACCAGTT AAAGAAACAA TTTGCTTATC TGTCAGGTGG AAGAGGGGGA
    GATGGATGCC CTGTTATCAC TTTTCCCGAC TACCCAGCTT TCAGCGAGAT CCCTGAGAAG
    GAGTTCCAGA ATGTCCTGAC CTACCTGACC AGCATCCCGA GTTTACGAGA TGCTGGAATC
    GGGTTTATTC TTGTCATAGA CCGCAGACAG GACAAATGGA CTTCTGTGAA AGCTTCCATA
    CTGCGGATAG CAGCATCTTT CCCAGGGAAC TTGCAACTCG TCCTCGTTCT GCGCCCAACA
    GGACTTATTC ATCGAGCTCT CTCAGACATT GCTTTCAAGT TCAACAGAGA TGAGTTTAAA
    ATGAAAGTAC CTATCATAAT GCTGGGCTCA GTATCAGAAC TGCAGGGTTA CATTGATAAA
    ACACAATTAA CAGAGGATCT TGGTGGCACC CTGGATTACT GCCATAACAG ATGGCTATCC
    CTTCGCACTG CTATAGAAGG TTTTGCCCAA AAGGTTAAGC AAACAGCTCA GATTCTTCAG
    TCCTTTGGGA CTGAACTGGC TGAAACAGAA CTACCAAATG ATGTGCAGTC AACCAGCTCC
    CTTCTTGCAA TACATACGGA AAAGAAGGAC AAAATGAAGG AAGATATAAG GTTAGCAGTA
    GAACAGGGAG ACGATATCCT TGGAAGTATT ACAGAGCCAG TTACTGAGAA CCCTGAATAC
    AAGCTGAATC AAGACCAACT AGACAATCAA ACTACAGTAG AAAGGCTTTT GGCTCAGTTA
    CATGAAACAG AGTCCGCCTT TGATGAGTTT TGGATTAAGC ACCAGCAAAA ACTTGAGCAG
    TGTCTGCACC TTCGGAATTT TGAACAGAAT TTTAGAGAGG TTAAAGCAGC TTTGGATATT
    GTGTCCGAGA GACTGTCTGC TTTCACAGAT GTGGGAAACA GCTGCTCACA TGTGGAGCAC
    ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA AAATCATGTG AAACTGTAGC AAAAGCCAGA
    ATGCTAGCTT CAGAGGGTGA TGCTTTTATT CAGAGCAATC ATTATGCTGT GGATTCCATT
    ATTCCAAAAT GCAGTGAGCT TCATCATCTA TGTGATGCCT TCACTACTGA GATGGAACAG
    AGGAGGAATG TTCTTAATAA ATCTCTAGAA CTGCATGGCT TACTGGAAAA GTCTATGAAG
    TGGTGCGATG AAGGAATTTA TCTTCTGGCT TCACAACCTG TAGATAAGTG TCAGTCTCAG
    GATGGTGCAG AATCAGCATT ACAGGAGATT GAGAAATTCC TAGACACTGG TGCAGGCAAT
    AAAATTAAGG AGTTGAATAA AGTTTACAGA GAGTATGCAC ACATCCTCAA TGAGGACCTA
    AAGGAGCATA TACAAAAGGT TTTCCAGAAG CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG
    AGACAAGTAA GTCTTAAGAA ACTGGCAGCT AAGCAAACAC GTCCTGTTCA GCCAGTTGCA
    CCTAGACCCG AAGCCTTTGT AAAATCTCCT TGTACCTCTC CAGGTCTTCA AAGAGATTGT
    GTATCCAACA CAGAAAGCGG TGTGCTTCGG AAAGTAAACT ACAGAAGAGG AAAGAGTGAA
    ATGAGTGAAC TCCATCAAAG CAGAGGAGGA TCTACAATGG ATGATGAAGA AAACCTAGCT
    GTGTTACGGC AGCATGTAAT GAATGAACTT ATTGAAACGG AACGAGCATA TGTGGAAGAA
    CTTCTCTGTG TTCTTGAGGG GTATGCTGCA GAAATGGACA ACCCCTTAAT GGCTCACCTC
    ATTTCACCAG AACTCCAAAA CAAGAAGGAT ATTTTGTTTG GGAATATGGA AGAAATTTAT
    CACTTTCACA ACAGGATATT CTTGAGAGAA CTAGAAAATT ATGTAGAATA CCCAGAACTG
    GTAGGACGGT GCTTTCTGGA TCAGATGGAA GATTTCCAGA TTTATGAGAA ATACTGTCAA
    AACAAACCTC GATCTGAAAG TTTGTGGAGG CAATTTTCAG ATTCCATATT CTTTCAGGAA
    TGTCAAAGGA AACTCGATCA CAAGCTCAGT TTGGACTCAT ACCTGCTGAA GCCAGTTCAA
    AGAATAACCA AATACCAATT ATTGCTAAAG GAGATGCTGA AGTACAGTAA GAACTGTGAA
    GGTGCTGAAG ATCTTCAGGA GGCATTAAAT TCTATATTGG GTATTCTCAA AGCAGTCAAT
    GATTCTATGC ACCAGATAGC CATCACAGGC TATGATGGAA ACCTGAATGA GCTGGGCAAG
    CTCCTAATGC AGGGATCCTT CAACGTCTGG ACTGATCATA AAAAGGGTCA CTCAAAGGTG
    AAAGATTTGG CACGCTTCAA GCCAATGCAG CGACACCTGT TCCTCCACGA GAAAGCAGTG
    CTGTTCTGTA AAAAGCGAGA AGAAAATGGA GAAGGATATG AGAAGGCACC TTCTTATAGC
    TATAAACACT CCTTAAATAT GGCAGCAGTT GGAATAACAG AAAATGTCAA AGGTGATGCA
    AAAAAATTTG AAATCTGGTA TAATGCAAGG GAAGAAGTTT ACATCATACA GGCACCAACT
    CCTGAAGTTA AAGCCACTTG GGTAAATGAA ATAAGAAAAG TGCTGACTAG TCAACTGCAG
    GCATGCAGAG AAGCCAGTCA ACATAGGACA CTTGAACACA CTCAGAGTTT ACCTCTACCA
    GCATCATCCT GTACAAGTCC TTCAAGGAAC AACATCAGAA ACGTGAAAAA AATGGATGAG
    AGAAAAGCTG ATCTCACAAG TCTAGAAGGT TATGTCACCA CAGTAGCACC AAAGTACCCC
    GAGAAAGGCA AAGATGACAC TAACTCTACC TCAGAATGCT CTGTACTGTC AAAAAAGCGC
    TTTACGCTGC AGGGCTTTAC TAACCTCAAA AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC
    AAAAAAGCTA AACGGCACGA AGTAGTGAGT GATCCAACTC CTTTTGGTGT AAGAGGTTGG
    GCTAAAACGT CTCATTCTCT TGATGCATCT GAAGAAAATG ATGGCTGGTC CAGTGCTGAG
    GAGCCGCTGA ATTCATCGGA TGCAGAAGAA GAAGGAGGAG GAGGAGGAGA GAGAAATGAG
    ATGAAGCTGA CTCCTGGTAA ATATACAGTT GTGTCTGACT ACGAGAAAGG AGTTTCTGAA
    GAATTTGCAG TGAAAGGTGG AGATCTAGTT CAACTGATTC GTGAAGGGGA GGAGGGACTT
    TGGTATGTGA AGAATCTGAC CACTGGTAGA GAAGGTTGGA TCCCAGCAAA CAATCTGCTG
    ATGCTCATAG GCAATTCAAA ATCAGCTCAA TCTTTGAGCA GCTCTGAATC AGGCACTGCT
    TCCAGCACTT TGAGCACGTC ATCAAGTTGC AGTGATAGCT GCAATGCCAG CAGCACCAGC
    TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30675
    Clone ID Related Accession (Same CDS sequence) XM_015277849.1 , XM_015277849.2
    Accession Version XM_015277849.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCAAATT TAAATGAAAC AGATGAAATC ATGCATCAGG ATATCATCCC CCTCTATGCA 
    GCAGATATTC ATGACCAGTT AAAGAAACAA TTTGCTTATC TGTCAGGTGG AAGAGGGGGA
    GATGGATGCC CTGTTATCAC TTTTCCCGAC TACCCAGCTT TCAGCGAGAT CCCTGAGAAG
    GAGTTCCAGA ATGTCCTGAC CTACCTGACC AGCATCCCGA GTTTACGAGA TGCTGGAATC
    GGGTTTATTC TTGTCATAGA CCGCAGACAG GACAAATGGA CTTCTGTGAA AGCTTCCATA
    CTGCGGATAG CAGCATCTTT CCCAGGGAAC TTGCAACTCG TCCTCGTTCT GCGCCCAACA
    GGACTTATTC ATCGAGCTCT CTCAGACATT GCTTTCAAGT TCAACAGAGA TGAGTTTAAA
    ATGAAAGTAC CTATCATAAT GCTGGGCTCA GTATCAGAAC TGCAGGGTTA CATTGATAAA
    ACACAATTAA CAGAGGATCT TGGTGGCACC CTGGATTACT GCCATAACAG ATGGCTATCC
    CTTCGCACTG CTATAGAAGG TTTTGCCCAA AAGGTTAAGC AAACAGCTCA GATTCTTCAG
    TCCTTTGGGA CTGAACTGGC TGAAACAGAA CTACCAAATG ATGTGCAGTC AACCAGCTCC
    CTTCTTGCAA TACATACGGA AAAGAAGGAC AAAATGAAGG AAGATATAAG GTTAGCAGTA
    GAACAGGGAG ACGATATCCT TGGAAGTATT ACAGAGCCAG TTACTGAGAA CCCTGAATAC
    AAGCTGAATC AAGACCAACT AGACAATCAA ACTACAGTAG AAAGGCTTTT GGCTCAGTTA
    CATGAAACAG AGTCCGCCTT TGATGAGTTT TGGATTAAGC ACCAGCAAAA ACTTGAGCAG
    TGTCTGCACC TTCGGAATTT TGAACAGAAT TTTAGAGAGG TTAAAGCAGC TTTGGATATT
    GTGTCCGAGA GACTGTCTGC TTTCACAGAT GTGGGAAACA GCTGCTCACA TGTGGAGCAC
    ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA AAATCATGTG AAACTGTAGC AAAAGCCAGA
    ATGCTAGCTT CAGAGGGTGA TGCTTTTATT CAGAGCAATC ATTATGCTGT GGATTCCATT
    ATTCCAAAAT GCAGTGAGCT TCATCATCTA TGTGATGCCT TCACTACTGA GATGGAACAG
    AGGAGGAATG TTCTTAATAA ATCTCTAGAA CTGCATGGCT TACTGGAAAA GTCTATGAAG
    TGGTGCGATG AAGGAATTTA TCTTCTGGCT TCACAACCTG TAGATAAGTG TCAGTCTCAG
    GATGGTGCAG AATCAGCATT ACAGGAGATT GAGAAATTCC TAGACACTGG TGCAGGCAAT
    AAAATTAAGG AGTTGAATAA AGTTTACAGA GAGTATGCAC ACATCCTCAA TGAGGACCTA
    AAGGAGCATA TACAAAAGGT TTTCCAGAAG CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG
    AGACAAGTAA GTCTTAAGAA ACTGGCAGCT AAGCAAACAC GTCCTGTTCA GCCAGTTGCA
    CCTAGACCCG AAGCCTTTGT AAAATCTCCT TGTACCTCTC CAGGTCTTCA AAGAGATTGT
    GTATCCAACA CAGAAAGCGG TGTGCTTCGG AAAGTAAACT ACAGAAGAGG AAAGAGTGAA
    ATGAGTGAAC TCCATCAAAG CAGAGGAGGA TCTACAATGG ATGATGAAGA AAACCTAGCT
    GTGTTACGGC AGCATGTAAT GAATGAACTT ATTGAAACGG AACGAGCATA TGTGGAAGAA
    CTTCTCTGTG TTCTTGAGGG GTATGCTGCA GAAATGGACA ACCCCTTAAT GGCTCACCTC
    ATTTCACCAG AACTCCAAAA CAAGAAGGAT ATTTTGTTTG GGAATATGGA AGAAATTTAT
    CACTTTCACA ACAGGATATT CTTGAGAGAA CTAGAAAATT ATGTAGAATA CCCAGAACTG
    GTAGGACGGT GCTTTCTGGA TCAGATGGAA GATTTCCAGA TTTATGAGAA ATACTGTCAA
    AACAAACCTC GATCTGAAAG TTTGTGGAGG CAATTTTCAG ATTCCATATT CTTTCAGGAA
    TGTCAAAGGA AACTCGATCA CAAGCTCAGT TTGGACTCAT ACCTGCTGAA GCCAGTTCAA
    AGAATAACCA AATACCAATT ATTGCTAAAG GAGATGCTGA AGTACAGTAA GAACTGTGAA
    GGTGCTGAAG ATCTTCAGGA GGCATTAAAT TCTATATTGG GTATTCTCAA AGCAGTCAAT
    GATTCTATGC ACCAGATAGC CATCACAGGC TATGATGGAA ACCTGAATGA GCTGGGCAAG
    CTCCTAATGC AGGGATCCTT CAACGTCTGG ACTGATCATA AAAAGGGTCA CTCAAAGGTG
    AAAGATTTGG CACGCTTCAA GCCAATGCAG CGACACCTGT TCCTCCACGA GAAAGCAGTG
    CTGTTCTGTA AAAAGCGAGA AGAAAATGGA GAAGGATATG AGAAGGCACC TTCTTATAGC
    TATAAACACT CCTTAAATAT GGCAGCAGTT GGAATAACAG AAAATGTCAA AGGTGATGCA
    AAAAAATTTG AAATCTGGTA TAATGCAAGG GAAGAAGTTT ACATCATACA GGCACCAACT
    CCTGAAGTTA AAGCCACTTG GGTAAATGAA ATAAGAAAAG TGCTGACTAG TCAACTGCAG
    GCATGCAGAG AAGCCAGTCA ACATAGGACA CTTGAACACA CTCAGAGTTT ACCTCTACCA
    GCATCATCCT GTACAAGTCC TTCAAGGAAC AACATCAGAA ACGTGAAAAA AATGGATGAG
    AGAAAAGCTG ATCTCACAAG TCTAGAAGGT TATGTCACCA CAGTAGCACC AAAGTACCCC
    GAGAAAGGCA AAGATGACAC TAACTCTACC TCAGAATGCT CTGTACTGTC AAAAAAGCGC
    TTTACGCTGC AGGGCTTTAC TAACCTCAAA AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC
    AAAAAAGCTA AACGGCACGA AGTAGTGAGT GATCCAACTC CTTTTGGTGT AAGAGGTTGG
    GCTAAAACGT CTCATTCTCT TGATGCATCT GAAGAAAATG ATGGCTGGTC CAGTGCTGAG
    GAGCCGCTGA ATTCATCGGA TGCAGAAGAA GAAGGAGGAG GAGGAGGAGA GAGAAATGAG
    ATGAAGCTGA CTCCTGGTAA ATATACAGTT GTGTCTGACT ACGAGAAAGG AGTTTCTGAA
    GAATTTGCAG TGAAAGGTGG AGATCTAGTT CAACTGATTC GTGAAGGGGA GGAGGGACTT
    TGGTATGTGA AGAATCTGAC CACTGGTAGA GAAGGTTGGA TCCCAGCAAA CAATCTGCTG
    ATGCTCATAG GCAATTCAAA ATCAGCTCAA TCTTTGAGCA GCTCTGAATC AGGCACTGCT
    TCCAGCACTT TGAGCACGTC ATCAAGTTGC AGTGATAGCT GCAATGCCAG CAGCACCAGC
    TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133335.1
    CDS85..3585
    Translation

    Target ORF information:

    RefSeq Version XM_015277849.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277849.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCAAATT TAAATGAAAC AGATGAAATC ATGCATCAGG ATATCATCCC CCTCTATGCA 
    GCAGATATTC ATGACCAGTT AAAGAAACAA TTTGCTTATC TGTCAGGTGG AAGAGGGGGA
    GATGGATGCC CTGTTATCAC TTTTCCCGAC TACCCAGCTT TCAGCGAGAT CCCTGAGAAG
    GAGTTCCAGA ATGTCCTGAC CTACCTGACC AGCATCCCGA GTTTACGAGA TGCTGGAATC
    GGGTTTATTC TTGTCATAGA CCGCAGACAG GACAAATGGA CTTCTGTGAA AGCTTCCATA
    CTGCGGATAG CAGCATCTTT CCCAGGGAAC TTGCAACTCG TCCTCGTTCT GCGCCCAACA
    GGACTTATTC ATCGAGCTCT CTCAGACATT GCTTTCAAGT TCAACAGAGA TGAGTTTAAA
    ATGAAAGTAC CTATCATAAT GCTGGGCTCA GTATCAGAAC TGCAGGGTTA CATTGATAAA
    ACACAATTAA CAGAGGATCT TGGTGGCACC CTGGATTACT GCCATAACAG ATGGCTATCC
    CTTCGCACTG CTATAGAAGG TTTTGCCCAA AAGGTTAAGC AAACAGCTCA GATTCTTCAG
    TCCTTTGGGA CTGAACTGGC TGAAACAGAA CTACCAAATG ATGTGCAGTC AACCAGCTCC
    CTTCTTGCAA TACATACGGA AAAGAAGGAC AAAATGAAGG AAGATATAAG GTTAGCAGTA
    GAACAGGGAG ACGATATCCT TGGAAGTATT ACAGAGCCAG TTACTGAGAA CCCTGAATAC
    AAGCTGAATC AAGACCAACT AGACAATCAA ACTACAGTAG AAAGGCTTTT GGCTCAGTTA
    CATGAAACAG AGTCCGCCTT TGATGAGTTT TGGATTAAGC ACCAGCAAAA ACTTGAGCAG
    TGTCTGCACC TTCGGAATTT TGAACAGAAT TTTAGAGAGG TTAAAGCAGC TTTGGATATT
    GTGTCCGAGA GACTGTCTGC TTTCACAGAT GTGGGAAACA GCTGCTCACA TGTGGAGCAC
    ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA AAATCATGTG AAACTGTAGC AAAAGCCAGA
    ATGCTAGCTT CAGAGGGTGA TGCTTTTATT CAGAGCAATC ATTATGCTGT GGATTCCATT
    ATTCCAAAAT GCAGTGAGCT TCATCATCTA TGTGATGCCT TCACTACTGA GATGGAACAG
    AGGAGGAATG TTCTTAATAA ATCTCTAGAA CTGCATGGCT TACTGGAAAA GTCTATGAAG
    TGGTGCGATG AAGGAATTTA TCTTCTGGCT TCACAACCTG TAGATAAGTG TCAGTCTCAG
    GATGGTGCAG AATCAGCATT ACAGGAGATT GAGAAATTCC TAGACACTGG TGCAGGCAAT
    AAAATTAAGG AGTTGAATAA AGTTTACAGA GAGTATGCAC ACATCCTCAA TGAGGACCTA
    AAGGAGCATA TACAAAAGGT TTTCCAGAAG CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG
    AGACAAGTAA GTCTTAAGAA ACTGGCAGCT AAGCAAACAC GTCCTGTTCA GCCAGTTGCA
    CCTAGACCCG AAGCCTTTGT AAAATCTCCT TGTACCTCTC CAGGTCTTCA AAGAGATTGT
    GTATCCAACA CAGAAAGCGG TGTGCTTCGG AAAGTAAACT ACAGAAGAGG AAAGAGTGAA
    ATGAGTGAAC TCCATCAAAG CAGAGGAGGA TCTACAATGG ATGATGAAGA AAACCTAGCT
    GTGTTACGGC AGCATGTAAT GAATGAACTT ATTGAAACGG AACGAGCATA TGTGGAAGAA
    CTTCTCTGTG TTCTTGAGGG GTATGCTGCA GAAATGGACA ACCCCTTAAT GGCTCACCTC
    ATTTCACCAG AACTCCAAAA CAAGAAGGAT ATTTTGTTTG GGAATATGGA AGAAATTTAT
    CACTTTCACA ACAGGATATT CTTGAGAGAA CTAGAAAATT ATGTAGAATA CCCAGAACTG
    GTAGGACGGT GCTTTCTGGA TCAGATGGAA GATTTCCAGA TTTATGAGAA ATACTGTCAA
    AACAAACCTC GATCTGAAAG TTTGTGGAGG CAATTTTCAG ATTCCATATT CTTTCAGGAA
    TGTCAAAGGA AACTCGATCA CAAGCTCAGT TTGGACTCAT ACCTGCTGAA GCCAGTTCAA
    AGAATAACCA AATACCAATT ATTGCTAAAG GAGATGCTGA AGTACAGTAA GAACTGTGAA
    GGTGCTGAAG ATCTTCAGGA GGCATTAAAT TCTATATTGG GTATTCTCAA AGCAGTCAAT
    GATTCTATGC ACCAGATAGC CATCACAGGC TATGATGGAA ACCTGAATGA GCTGGGCAAG
    CTCCTAATGC AGGGATCCTT CAACGTCTGG ACTGATCATA AAAAGGGTCA CTCAAAGGTG
    AAAGATTTGG CACGCTTCAA GCCAATGCAG CGACACCTGT TCCTCCACGA GAAAGCAGTG
    CTGTTCTGTA AAAAGCGAGA AGAAAATGGA GAAGGATATG AGAAGGCACC TTCTTATAGC
    TATAAACACT CCTTAAATAT GGCAGCAGTT GGAATAACAG AAAATGTCAA AGGTGATGCA
    AAAAAATTTG AAATCTGGTA TAATGCAAGG GAAGAAGTTT ACATCATACA GGCACCAACT
    CCTGAAGTTA AAGCCACTTG GGTAAATGAA ATAAGAAAAG TGCTGACTAG TCAACTGCAG
    GCATGCAGAG AAGCCAGTCA ACATAGGACA CTTGAACACA CTCAGAGTTT ACCTCTACCA
    GCATCATCCT GTACAAGTCC TTCAAGGAAC AACATCAGAA ACGTGAAAAA AATGGATGAG
    AGAAAAGCTG ATCTCACAAG TCTAGAAGGT TATGTCACCA CAGTAGCACC AAAGTACCCC
    GAGAAAGGCA AAGATGACAC TAACTCTACC TCAGAATGCT CTGTACTGTC AAAAAAGCGC
    TTTACGCTGC AGGGCTTTAC TAACCTCAAA AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC
    AAAAAAGCTA AACGGCACGA AGTAGTGAGT GATCCAACTC CTTTTGGTGT AAGAGGTTGG
    GCTAAAACGT CTCATTCTCT TGATGCATCT GAAGAAAATG ATGGCTGGTC CAGTGCTGAG
    GAGCCGCTGA ATTCATCGGA TGCAGAAGAA GAAGGAGGAG GAGGAGGAGA GAGAAATGAG
    ATGAAGCTGA CTCCTGGTAA ATATACAGTT GTGTCTGACT ACGAGAAAGG AGTTTCTGAA
    GAATTTGCAG TGAAAGGTGG AGATCTAGTT CAACTGATTC GTGAAGGGGA GGAGGGACTT
    TGGTATGTGA AGAATCTGAC CACTGGTAGA GAAGGTTGGA TCCCAGCAAA CAATCTGCTG
    ATGCTCATAG GCAATTCAAA ATCAGCTCAA TCTTTGAGCA GCTCTGAATC AGGCACTGCT
    TCCAGCACTT TGAGCACGTC ATCAAGTTGC AGTGATAGCT GCAATGCCAG CAGCACCAGC
    TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30676
    Clone ID Related Accession (Same CDS sequence) XM_015277850.1 , XM_015277850.2
    Accession Version XM_015277850.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCTGACT ATTGGTACTG GAGGCTGGTC CCGTGCAGAG AAACCGGCAG CTGTAACTGT 
    TTTGCAACAG CCAACCAATC TAATGAGGAG AAAGAAGAAG ACACGAACCA CCAGGTTGTG
    GTGTCAGACA TCATGCAGCT GGTGCGAGAC AAGCAGGAGG TGCCGGAAAG AACAGCAGAT
    GAAATCATGC ATCAGGATAT CATCCCCCTC TATGCAGCAG ATATTCATGA CCAGTTAAAG
    AAACAATTTG CTTATCTGTC AGGTGGAAGA GGGGGAGATG GATGCCCTGT TATCACTTTT
    CCCGACTACC CAGCTTTCAG CGAGATCCCT GAGAAGGAGT TCCAGAATGT CCTGACCTAC
    CTGACCAGCA TCCCGAGTTT ACGAGATGCT GGAATCGGGT TTATTCTTGT CATAGACCGC
    AGACAGGACA AATGGACTTC TGTGAAAGCT TCCATACTGC GGATAGCAGC ATCTTTCCCA
    GGGAACTTGC AACTCGTCCT CGTTCTGCGC CCAACAGGAC TTATTCATCG AGCTCTCTCA
    GACATTGCTT TCAAGTTCAA CAGAGATGAG TTTAAAATGA AAGTACCTAT CATAATGCTG
    GGCTCAGTAT CAGAACTGCA GGGTTACATT GATAAAACAC AATTAACAGA GGATCTTGGT
    GGCACCCTGG ATTACTGCCA TAACAGATGG CTATCCCTTC GCACTGCTAT AGAAGGTTTT
    GCCCAAAAGG TTAAGCAAAC AGCTCAGATT CTTCAGTCCT TTGGGACTGA ACTGGCTGAA
    ACAGAACTAC CAAATGATGT GCAGTCAACC AGCTCCCTTC TTGCAATACA TACGGAAAAG
    AAGGACAAAA TGAAGGAAGA TATAAGGTTA GCAGTAGAAC AGGGAGACGA TATCCTTGGA
    AGTATTACAG AGCCAGTTAC TGAGAACCCT GAATACAAGC TGAATCAAGA CCAACTAGAC
    AATCAAACTA CAGTAGAAAG GCTTTTGGCT CAGTTACATG AAACAGAGTC CGCCTTTGAT
    GAGTTTTGGA TTAAGCACCA GCAAAAACTT GAGCAGTGTC TGCACCTTCG GAATTTTGAA
    CAGAATTTTA GAGAGGTTAA AGCAGCTTTG GATATTGTGT CCGAGAGACT GTCTGCTTTC
    ACAGATGTGG GAAACAGCTG CTCACATGTG GAGCACATTT TGAAAGATCT TGCCAACTTT
    GAAGAAAAAT CATGTGAAAC TGTAGCAAAA GCCAGAATGC TAGCTTCAGA GGGTGATGCT
    TTTATTCAGA GCAATCATTA TGCTGTGGAT TCCATTATTC CAAAATGCAG TGAGCTTCAT
    CATCTATGTG ATGCCTTCAC TACTGAGATG GAACAGAGGA GGAATGTTCT TAATAAATCT
    CTAGAACTGC ATGGCTTACT GGAAAAGTCT ATGAAGTGGT GCGATGAAGG AATTTATCTT
    CTGGCTTCAC AACCTGTAGA TAAGTGTCAG TCTCAGGATG GTGCAGAATC AGCATTACAG
    GAGATTGAGA AATTCCTAGA CACTGGTGCA GGCAATAAAA TTAAGGAGTT GAATAAAGTT
    TACAGAGAGT ATGCACACAT CCTCAATGAG GACCTAAAGG AGCATATACA AAAGGTTTTC
    CAGAAGCAAG AAAGCATGGA AGAAATGTTT CAAAAGAGAC AAGTAAGTCT TAAGAAACTG
    GCAGCTAAGC AAACACGTCC TGTTCAGCCA GTTGCACCTA GACCCGAAGC CTTTGTAAAA
    TCTCCTTGTA CCTCTCCAGG TCTTCAAAGA GATTGTGTAT CCAACACAGA AAGCGGTGTG
    CTTCGGAAAG TAAACTACAG AAGAGGAAAG AGTGAAATGA GTGAACTCCA TCAAAGCAGA
    GGAGGATCTA CAATGGATGA TGAAGAAAAC CTAGCTGTGT TACGGCAGCA TGTAATGAAT
    GAACTTATTG AAACGGAACG AGCATATGTG GAAGAACTTC TCTGTGTTCT TGAGGGGTAT
    GCTGCAGAAA TGGACAACCC CTTAATGGCT CACCTCATTT CACCAGAACT CCAAAACAAG
    AAGGATATTT TGTTTGGGAA TATGGAAGAA ATTTATCACT TTCACAACAG GATATTCTTG
    AGAGAACTAG AAAATTATGT AGAATACCCA GAACTGGTAG GACGGTGCTT TCTGGATCAG
    ATGGAAGATT TCCAGATTTA TGAGAAATAC TGTCAAAACA AACCTCGATC TGAAAGTTTG
    TGGAGGCAAT TTTCAGATTC CATATTCTTT CAGGAATGTC AAAGGAAACT CGATCACAAG
    CTCAGTTTGG ACTCATACCT GCTGAAGCCA GTTCAAAGAA TAACCAAATA CCAATTATTG
    CTAAAGGAGA TGCTGAAGTA CAGTAAGAAC TGTGAAGGTG CTGAAGATCT TCAGGAGGCA
    TTAAATTCTA TATTGGGTAT TCTCAAAGCA GTCAATGATT CTATGCACCA GATAGCCATC
    ACAGGCTATG ATGGAAACCT GAATGAGCTG GGCAAGCTCC TAATGCAGGG ATCCTTCAAC
    GTCTGGACTG ATCATAAAAA GGGTCACTCA AAGGTGAAAG ATTTGGCACG CTTCAAGCCA
    ATGCAGCGAC ACCTGTTCCT CCACGAGAAA GCAGTGCTGT TCTGTAAAAA GCGAGAAGAA
    AATGGAGAAG GATATGAGAA GGCACCTTCT TATAGCTATA AACACTCCTT AAATATGGCA
    GCAGTTGGAA TAACAGAAAA TGTCAAAGGT GATGCAAAAA AATTTGAAAT CTGGTATAAT
    GCAAGGGAAG AAGTTTACAT CATACAGGCA CCAACTCCTG AAGTTAAAGC CACTTGGGTA
    AATGAAATAA GAAAAGTGCT GACTAGTCAA CTGCAGGCAT GCAGAGAAGC CAGTCAACAT
    AGGACACTTG AACACACTCA GAGTTTACCT CTACCAGCAT CATCCTGTAC AAGTCCTTCA
    AGGAACAACA TCAGAAACGT GAAAAAAATG GATGAGAGAA AAGCTGATCT CACAAGTCTA
    GAAGGTTATG TCACCACAGT AGCACCAAAG TACCCCGAGA AAGGCAAAGG TTGGGCTAAA
    ACGTCTCATT CTCTTGATGC ATCTGAAGAA AATGATGGCT GGTCCAGTGC TGAGGAGCCG
    CTGAATTCAT CGGATGCAGA AGAAGAAGGA GGAGGAGGAG GAGAGAGAAA TGAGATGAAG
    CTGACTCCTG GTAAATATAC AGTTGTGTCT GACTACGAGA AAGGAGTTTC TGAAGAATTT
    GCAGTGAAAG GTGGAGATCT AGTTCAACTG ATTCGTGAAG GGGAGGAGGG ACTTTGGTAT
    GTGAAGAATC TGACCACTGG TAGAGAAGGT TGGATCCCAG CAAACAATCT GCTGATGCTC
    ATAGGCAATT CAAAATCAGC TCAATCTTTG AGCAGCTCTG AATCAGGCAC TGCTTCCAGC
    ACTTTGAGCA CGTCATCAAG TTGCAGTGAT AGCTGCAATG CCAGCAGCAC CAGCTTCTCA
    GACATTAAGG GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133336.1
    CDS116..3610
    Translation

    Target ORF information:

    RefSeq Version XM_015277850.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277850.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCTGACT ATTGGTACTG GAGGCTGGTC CCGTGCAGAG AAACCGGCAG CTGTAACTGT 
    TTTGCAACAG CCAACCAATC TAATGAGGAG AAAGAAGAAG ACACGAACCA CCAGGTTGTG
    GTGTCAGACA TCATGCAGCT GGTGCGAGAC AAGCAGGAGG TGCCGGAAAG AACAGCAGAT
    GAAATCATGC ATCAGGATAT CATCCCCCTC TATGCAGCAG ATATTCATGA CCAGTTAAAG
    AAACAATTTG CTTATCTGTC AGGTGGAAGA GGGGGAGATG GATGCCCTGT TATCACTTTT
    CCCGACTACC CAGCTTTCAG CGAGATCCCT GAGAAGGAGT TCCAGAATGT CCTGACCTAC
    CTGACCAGCA TCCCGAGTTT ACGAGATGCT GGAATCGGGT TTATTCTTGT CATAGACCGC
    AGACAGGACA AATGGACTTC TGTGAAAGCT TCCATACTGC GGATAGCAGC ATCTTTCCCA
    GGGAACTTGC AACTCGTCCT CGTTCTGCGC CCAACAGGAC TTATTCATCG AGCTCTCTCA
    GACATTGCTT TCAAGTTCAA CAGAGATGAG TTTAAAATGA AAGTACCTAT CATAATGCTG
    GGCTCAGTAT CAGAACTGCA GGGTTACATT GATAAAACAC AATTAACAGA GGATCTTGGT
    GGCACCCTGG ATTACTGCCA TAACAGATGG CTATCCCTTC GCACTGCTAT AGAAGGTTTT
    GCCCAAAAGG TTAAGCAAAC AGCTCAGATT CTTCAGTCCT TTGGGACTGA ACTGGCTGAA
    ACAGAACTAC CAAATGATGT GCAGTCAACC AGCTCCCTTC TTGCAATACA TACGGAAAAG
    AAGGACAAAA TGAAGGAAGA TATAAGGTTA GCAGTAGAAC AGGGAGACGA TATCCTTGGA
    AGTATTACAG AGCCAGTTAC TGAGAACCCT GAATACAAGC TGAATCAAGA CCAACTAGAC
    AATCAAACTA CAGTAGAAAG GCTTTTGGCT CAGTTACATG AAACAGAGTC CGCCTTTGAT
    GAGTTTTGGA TTAAGCACCA GCAAAAACTT GAGCAGTGTC TGCACCTTCG GAATTTTGAA
    CAGAATTTTA GAGAGGTTAA AGCAGCTTTG GATATTGTGT CCGAGAGACT GTCTGCTTTC
    ACAGATGTGG GAAACAGCTG CTCACATGTG GAGCACATTT TGAAAGATCT TGCCAACTTT
    GAAGAAAAAT CATGTGAAAC TGTAGCAAAA GCCAGAATGC TAGCTTCAGA GGGTGATGCT
    TTTATTCAGA GCAATCATTA TGCTGTGGAT TCCATTATTC CAAAATGCAG TGAGCTTCAT
    CATCTATGTG ATGCCTTCAC TACTGAGATG GAACAGAGGA GGAATGTTCT TAATAAATCT
    CTAGAACTGC ATGGCTTACT GGAAAAGTCT ATGAAGTGGT GCGATGAAGG AATTTATCTT
    CTGGCTTCAC AACCTGTAGA TAAGTGTCAG TCTCAGGATG GTGCAGAATC AGCATTACAG
    GAGATTGAGA AATTCCTAGA CACTGGTGCA GGCAATAAAA TTAAGGAGTT GAATAAAGTT
    TACAGAGAGT ATGCACACAT CCTCAATGAG GACCTAAAGG AGCATATACA AAAGGTTTTC
    CAGAAGCAAG AAAGCATGGA AGAAATGTTT CAAAAGAGAC AAGTAAGTCT TAAGAAACTG
    GCAGCTAAGC AAACACGTCC TGTTCAGCCA GTTGCACCTA GACCCGAAGC CTTTGTAAAA
    TCTCCTTGTA CCTCTCCAGG TCTTCAAAGA GATTGTGTAT CCAACACAGA AAGCGGTGTG
    CTTCGGAAAG TAAACTACAG AAGAGGAAAG AGTGAAATGA GTGAACTCCA TCAAAGCAGA
    GGAGGATCTA CAATGGATGA TGAAGAAAAC CTAGCTGTGT TACGGCAGCA TGTAATGAAT
    GAACTTATTG AAACGGAACG AGCATATGTG GAAGAACTTC TCTGTGTTCT TGAGGGGTAT
    GCTGCAGAAA TGGACAACCC CTTAATGGCT CACCTCATTT CACCAGAACT CCAAAACAAG
    AAGGATATTT TGTTTGGGAA TATGGAAGAA ATTTATCACT TTCACAACAG GATATTCTTG
    AGAGAACTAG AAAATTATGT AGAATACCCA GAACTGGTAG GACGGTGCTT TCTGGATCAG
    ATGGAAGATT TCCAGATTTA TGAGAAATAC TGTCAAAACA AACCTCGATC TGAAAGTTTG
    TGGAGGCAAT TTTCAGATTC CATATTCTTT CAGGAATGTC AAAGGAAACT CGATCACAAG
    CTCAGTTTGG ACTCATACCT GCTGAAGCCA GTTCAAAGAA TAACCAAATA CCAATTATTG
    CTAAAGGAGA TGCTGAAGTA CAGTAAGAAC TGTGAAGGTG CTGAAGATCT TCAGGAGGCA
    TTAAATTCTA TATTGGGTAT TCTCAAAGCA GTCAATGATT CTATGCACCA GATAGCCATC
    ACAGGCTATG ATGGAAACCT GAATGAGCTG GGCAAGCTCC TAATGCAGGG ATCCTTCAAC
    GTCTGGACTG ATCATAAAAA GGGTCACTCA AAGGTGAAAG ATTTGGCACG CTTCAAGCCA
    ATGCAGCGAC ACCTGTTCCT CCACGAGAAA GCAGTGCTGT TCTGTAAAAA GCGAGAAGAA
    AATGGAGAAG GATATGAGAA GGCACCTTCT TATAGCTATA AACACTCCTT AAATATGGCA
    GCAGTTGGAA TAACAGAAAA TGTCAAAGGT GATGCAAAAA AATTTGAAAT CTGGTATAAT
    GCAAGGGAAG AAGTTTACAT CATACAGGCA CCAACTCCTG AAGTTAAAGC CACTTGGGTA
    AATGAAATAA GAAAAGTGCT GACTAGTCAA CTGCAGGCAT GCAGAGAAGC CAGTCAACAT
    AGGACACTTG AACACACTCA GAGTTTACCT CTACCAGCAT CATCCTGTAC AAGTCCTTCA
    AGGAACAACA TCAGAAACGT GAAAAAAATG GATGAGAGAA AAGCTGATCT CACAAGTCTA
    GAAGGTTATG TCACCACAGT AGCACCAAAG TACCCCGAGA AAGGCAAAGG TTGGGCTAAA
    ACGTCTCATT CTCTTGATGC ATCTGAAGAA AATGATGGCT GGTCCAGTGC TGAGGAGCCG
    CTGAATTCAT CGGATGCAGA AGAAGAAGGA GGAGGAGGAG GAGAGAGAAA TGAGATGAAG
    CTGACTCCTG GTAAATATAC AGTTGTGTCT GACTACGAGA AAGGAGTTTC TGAAGAATTT
    GCAGTGAAAG GTGGAGATCT AGTTCAACTG ATTCGTGAAG GGGAGGAGGG ACTTTGGTAT
    GTGAAGAATC TGACCACTGG TAGAGAAGGT TGGATCCCAG CAAACAATCT GCTGATGCTC
    ATAGGCAATT CAAAATCAGC TCAATCTTTG AGCAGCTCTG AATCAGGCAC TGCTTCCAGC
    ACTTTGAGCA CGTCATCAAG TTGCAGTGAT AGCTGCAATG CCAGCAGCAC CAGCTTCTCA
    GACATTAAGG GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30676
    Clone ID Related Accession (Same CDS sequence) XM_015277850.1 , XM_015277850.2
    Accession Version XM_015277850.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCTGACT ATTGGTACTG GAGGCTGGTC CCGTGCAGAG AAACCGGCAG CTGTAACTGT 
    TTTGCAACAG CCAACCAATC TAATGAGGAG AAAGAAGAAG ACACGAACCA CCAGGTTGTG
    GTGTCAGACA TCATGCAGCT GGTGCGAGAC AAGCAGGAGG TGCCGGAAAG AACAGCAGAT
    GAAATCATGC ATCAGGATAT CATCCCCCTC TATGCAGCAG ATATTCATGA CCAGTTAAAG
    AAACAATTTG CTTATCTGTC AGGTGGAAGA GGGGGAGATG GATGCCCTGT TATCACTTTT
    CCCGACTACC CAGCTTTCAG CGAGATCCCT GAGAAGGAGT TCCAGAATGT CCTGACCTAC
    CTGACCAGCA TCCCGAGTTT ACGAGATGCT GGAATCGGGT TTATTCTTGT CATAGACCGC
    AGACAGGACA AATGGACTTC TGTGAAAGCT TCCATACTGC GGATAGCAGC ATCTTTCCCA
    GGGAACTTGC AACTCGTCCT CGTTCTGCGC CCAACAGGAC TTATTCATCG AGCTCTCTCA
    GACATTGCTT TCAAGTTCAA CAGAGATGAG TTTAAAATGA AAGTACCTAT CATAATGCTG
    GGCTCAGTAT CAGAACTGCA GGGTTACATT GATAAAACAC AATTAACAGA GGATCTTGGT
    GGCACCCTGG ATTACTGCCA TAACAGATGG CTATCCCTTC GCACTGCTAT AGAAGGTTTT
    GCCCAAAAGG TTAAGCAAAC AGCTCAGATT CTTCAGTCCT TTGGGACTGA ACTGGCTGAA
    ACAGAACTAC CAAATGATGT GCAGTCAACC AGCTCCCTTC TTGCAATACA TACGGAAAAG
    AAGGACAAAA TGAAGGAAGA TATAAGGTTA GCAGTAGAAC AGGGAGACGA TATCCTTGGA
    AGTATTACAG AGCCAGTTAC TGAGAACCCT GAATACAAGC TGAATCAAGA CCAACTAGAC
    AATCAAACTA CAGTAGAAAG GCTTTTGGCT CAGTTACATG AAACAGAGTC CGCCTTTGAT
    GAGTTTTGGA TTAAGCACCA GCAAAAACTT GAGCAGTGTC TGCACCTTCG GAATTTTGAA
    CAGAATTTTA GAGAGGTTAA AGCAGCTTTG GATATTGTGT CCGAGAGACT GTCTGCTTTC
    ACAGATGTGG GAAACAGCTG CTCACATGTG GAGCACATTT TGAAAGATCT TGCCAACTTT
    GAAGAAAAAT CATGTGAAAC TGTAGCAAAA GCCAGAATGC TAGCTTCAGA GGGTGATGCT
    TTTATTCAGA GCAATCATTA TGCTGTGGAT TCCATTATTC CAAAATGCAG TGAGCTTCAT
    CATCTATGTG ATGCCTTCAC TACTGAGATG GAACAGAGGA GGAATGTTCT TAATAAATCT
    CTAGAACTGC ATGGCTTACT GGAAAAGTCT ATGAAGTGGT GCGATGAAGG AATTTATCTT
    CTGGCTTCAC AACCTGTAGA TAAGTGTCAG TCTCAGGATG GTGCAGAATC AGCATTACAG
    GAGATTGAGA AATTCCTAGA CACTGGTGCA GGCAATAAAA TTAAGGAGTT GAATAAAGTT
    TACAGAGAGT ATGCACACAT CCTCAATGAG GACCTAAAGG AGCATATACA AAAGGTTTTC
    CAGAAGCAAG AAAGCATGGA AGAAATGTTT CAAAAGAGAC AAGTAAGTCT TAAGAAACTG
    GCAGCTAAGC AAACACGTCC TGTTCAGCCA GTTGCACCTA GACCCGAAGC CTTTGTAAAA
    TCTCCTTGTA CCTCTCCAGG TCTTCAAAGA GATTGTGTAT CCAACACAGA AAGCGGTGTG
    CTTCGGAAAG TAAACTACAG AAGAGGAAAG AGTGAAATGA GTGAACTCCA TCAAAGCAGA
    GGAGGATCTA CAATGGATGA TGAAGAAAAC CTAGCTGTGT TACGGCAGCA TGTAATGAAT
    GAACTTATTG AAACGGAACG AGCATATGTG GAAGAACTTC TCTGTGTTCT TGAGGGGTAT
    GCTGCAGAAA TGGACAACCC CTTAATGGCT CACCTCATTT CACCAGAACT CCAAAACAAG
    AAGGATATTT TGTTTGGGAA TATGGAAGAA ATTTATCACT TTCACAACAG GATATTCTTG
    AGAGAACTAG AAAATTATGT AGAATACCCA GAACTGGTAG GACGGTGCTT TCTGGATCAG
    ATGGAAGATT TCCAGATTTA TGAGAAATAC TGTCAAAACA AACCTCGATC TGAAAGTTTG
    TGGAGGCAAT TTTCAGATTC CATATTCTTT CAGGAATGTC AAAGGAAACT CGATCACAAG
    CTCAGTTTGG ACTCATACCT GCTGAAGCCA GTTCAAAGAA TAACCAAATA CCAATTATTG
    CTAAAGGAGA TGCTGAAGTA CAGTAAGAAC TGTGAAGGTG CTGAAGATCT TCAGGAGGCA
    TTAAATTCTA TATTGGGTAT TCTCAAAGCA GTCAATGATT CTATGCACCA GATAGCCATC
    ACAGGCTATG ATGGAAACCT GAATGAGCTG GGCAAGCTCC TAATGCAGGG ATCCTTCAAC
    GTCTGGACTG ATCATAAAAA GGGTCACTCA AAGGTGAAAG ATTTGGCACG CTTCAAGCCA
    ATGCAGCGAC ACCTGTTCCT CCACGAGAAA GCAGTGCTGT TCTGTAAAAA GCGAGAAGAA
    AATGGAGAAG GATATGAGAA GGCACCTTCT TATAGCTATA AACACTCCTT AAATATGGCA
    GCAGTTGGAA TAACAGAAAA TGTCAAAGGT GATGCAAAAA AATTTGAAAT CTGGTATAAT
    GCAAGGGAAG AAGTTTACAT CATACAGGCA CCAACTCCTG AAGTTAAAGC CACTTGGGTA
    AATGAAATAA GAAAAGTGCT GACTAGTCAA CTGCAGGCAT GCAGAGAAGC CAGTCAACAT
    AGGACACTTG AACACACTCA GAGTTTACCT CTACCAGCAT CATCCTGTAC AAGTCCTTCA
    AGGAACAACA TCAGAAACGT GAAAAAAATG GATGAGAGAA AAGCTGATCT CACAAGTCTA
    GAAGGTTATG TCACCACAGT AGCACCAAAG TACCCCGAGA AAGGCAAAGG TTGGGCTAAA
    ACGTCTCATT CTCTTGATGC ATCTGAAGAA AATGATGGCT GGTCCAGTGC TGAGGAGCCG
    CTGAATTCAT CGGATGCAGA AGAAGAAGGA GGAGGAGGAG GAGAGAGAAA TGAGATGAAG
    CTGACTCCTG GTAAATATAC AGTTGTGTCT GACTACGAGA AAGGAGTTTC TGAAGAATTT
    GCAGTGAAAG GTGGAGATCT AGTTCAACTG ATTCGTGAAG GGGAGGAGGG ACTTTGGTAT
    GTGAAGAATC TGACCACTGG TAGAGAAGGT TGGATCCCAG CAAACAATCT GCTGATGCTC
    ATAGGCAATT CAAAATCAGC TCAATCTTTG AGCAGCTCTG AATCAGGCAC TGCTTCCAGC
    ACTTTGAGCA CGTCATCAAG TTGCAGTGAT AGCTGCAATG CCAGCAGCAC CAGCTTCTCA
    GACATTAAGG GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133336.1
    CDS129..3623
    Translation

    Target ORF information:

    RefSeq Version XM_015277850.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277850.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCTGACT ATTGGTACTG GAGGCTGGTC CCGTGCAGAG AAACCGGCAG CTGTAACTGT 
    TTTGCAACAG CCAACCAATC TAATGAGGAG AAAGAAGAAG ACACGAACCA CCAGGTTGTG
    GTGTCAGACA TCATGCAGCT GGTGCGAGAC AAGCAGGAGG TGCCGGAAAG AACAGCAGAT
    GAAATCATGC ATCAGGATAT CATCCCCCTC TATGCAGCAG ATATTCATGA CCAGTTAAAG
    AAACAATTTG CTTATCTGTC AGGTGGAAGA GGGGGAGATG GATGCCCTGT TATCACTTTT
    CCCGACTACC CAGCTTTCAG CGAGATCCCT GAGAAGGAGT TCCAGAATGT CCTGACCTAC
    CTGACCAGCA TCCCGAGTTT ACGAGATGCT GGAATCGGGT TTATTCTTGT CATAGACCGC
    AGACAGGACA AATGGACTTC TGTGAAAGCT TCCATACTGC GGATAGCAGC ATCTTTCCCA
    GGGAACTTGC AACTCGTCCT CGTTCTGCGC CCAACAGGAC TTATTCATCG AGCTCTCTCA
    GACATTGCTT TCAAGTTCAA CAGAGATGAG TTTAAAATGA AAGTACCTAT CATAATGCTG
    GGCTCAGTAT CAGAACTGCA GGGTTACATT GATAAAACAC AATTAACAGA GGATCTTGGT
    GGCACCCTGG ATTACTGCCA TAACAGATGG CTATCCCTTC GCACTGCTAT AGAAGGTTTT
    GCCCAAAAGG TTAAGCAAAC AGCTCAGATT CTTCAGTCCT TTGGGACTGA ACTGGCTGAA
    ACAGAACTAC CAAATGATGT GCAGTCAACC AGCTCCCTTC TTGCAATACA TACGGAAAAG
    AAGGACAAAA TGAAGGAAGA TATAAGGTTA GCAGTAGAAC AGGGAGACGA TATCCTTGGA
    AGTATTACAG AGCCAGTTAC TGAGAACCCT GAATACAAGC TGAATCAAGA CCAACTAGAC
    AATCAAACTA CAGTAGAAAG GCTTTTGGCT CAGTTACATG AAACAGAGTC CGCCTTTGAT
    GAGTTTTGGA TTAAGCACCA GCAAAAACTT GAGCAGTGTC TGCACCTTCG GAATTTTGAA
    CAGAATTTTA GAGAGGTTAA AGCAGCTTTG GATATTGTGT CCGAGAGACT GTCTGCTTTC
    ACAGATGTGG GAAACAGCTG CTCACATGTG GAGCACATTT TGAAAGATCT TGCCAACTTT
    GAAGAAAAAT CATGTGAAAC TGTAGCAAAA GCCAGAATGC TAGCTTCAGA GGGTGATGCT
    TTTATTCAGA GCAATCATTA TGCTGTGGAT TCCATTATTC CAAAATGCAG TGAGCTTCAT
    CATCTATGTG ATGCCTTCAC TACTGAGATG GAACAGAGGA GGAATGTTCT TAATAAATCT
    CTAGAACTGC ATGGCTTACT GGAAAAGTCT ATGAAGTGGT GCGATGAAGG AATTTATCTT
    CTGGCTTCAC AACCTGTAGA TAAGTGTCAG TCTCAGGATG GTGCAGAATC AGCATTACAG
    GAGATTGAGA AATTCCTAGA CACTGGTGCA GGCAATAAAA TTAAGGAGTT GAATAAAGTT
    TACAGAGAGT ATGCACACAT CCTCAATGAG GACCTAAAGG AGCATATACA AAAGGTTTTC
    CAGAAGCAAG AAAGCATGGA AGAAATGTTT CAAAAGAGAC AAGTAAGTCT TAAGAAACTG
    GCAGCTAAGC AAACACGTCC TGTTCAGCCA GTTGCACCTA GACCCGAAGC CTTTGTAAAA
    TCTCCTTGTA CCTCTCCAGG TCTTCAAAGA GATTGTGTAT CCAACACAGA AAGCGGTGTG
    CTTCGGAAAG TAAACTACAG AAGAGGAAAG AGTGAAATGA GTGAACTCCA TCAAAGCAGA
    GGAGGATCTA CAATGGATGA TGAAGAAAAC CTAGCTGTGT TACGGCAGCA TGTAATGAAT
    GAACTTATTG AAACGGAACG AGCATATGTG GAAGAACTTC TCTGTGTTCT TGAGGGGTAT
    GCTGCAGAAA TGGACAACCC CTTAATGGCT CACCTCATTT CACCAGAACT CCAAAACAAG
    AAGGATATTT TGTTTGGGAA TATGGAAGAA ATTTATCACT TTCACAACAG GATATTCTTG
    AGAGAACTAG AAAATTATGT AGAATACCCA GAACTGGTAG GACGGTGCTT TCTGGATCAG
    ATGGAAGATT TCCAGATTTA TGAGAAATAC TGTCAAAACA AACCTCGATC TGAAAGTTTG
    TGGAGGCAAT TTTCAGATTC CATATTCTTT CAGGAATGTC AAAGGAAACT CGATCACAAG
    CTCAGTTTGG ACTCATACCT GCTGAAGCCA GTTCAAAGAA TAACCAAATA CCAATTATTG
    CTAAAGGAGA TGCTGAAGTA CAGTAAGAAC TGTGAAGGTG CTGAAGATCT TCAGGAGGCA
    TTAAATTCTA TATTGGGTAT TCTCAAAGCA GTCAATGATT CTATGCACCA GATAGCCATC
    ACAGGCTATG ATGGAAACCT GAATGAGCTG GGCAAGCTCC TAATGCAGGG ATCCTTCAAC
    GTCTGGACTG ATCATAAAAA GGGTCACTCA AAGGTGAAAG ATTTGGCACG CTTCAAGCCA
    ATGCAGCGAC ACCTGTTCCT CCACGAGAAA GCAGTGCTGT TCTGTAAAAA GCGAGAAGAA
    AATGGAGAAG GATATGAGAA GGCACCTTCT TATAGCTATA AACACTCCTT AAATATGGCA
    GCAGTTGGAA TAACAGAAAA TGTCAAAGGT GATGCAAAAA AATTTGAAAT CTGGTATAAT
    GCAAGGGAAG AAGTTTACAT CATACAGGCA CCAACTCCTG AAGTTAAAGC CACTTGGGTA
    AATGAAATAA GAAAAGTGCT GACTAGTCAA CTGCAGGCAT GCAGAGAAGC CAGTCAACAT
    AGGACACTTG AACACACTCA GAGTTTACCT CTACCAGCAT CATCCTGTAC AAGTCCTTCA
    AGGAACAACA TCAGAAACGT GAAAAAAATG GATGAGAGAA AAGCTGATCT CACAAGTCTA
    GAAGGTTATG TCACCACAGT AGCACCAAAG TACCCCGAGA AAGGCAAAGG TTGGGCTAAA
    ACGTCTCATT CTCTTGATGC ATCTGAAGAA AATGATGGCT GGTCCAGTGC TGAGGAGCCG
    CTGAATTCAT CGGATGCAGA AGAAGAAGGA GGAGGAGGAG GAGAGAGAAA TGAGATGAAG
    CTGACTCCTG GTAAATATAC AGTTGTGTCT GACTACGAGA AAGGAGTTTC TGAAGAATTT
    GCAGTGAAAG GTGGAGATCT AGTTCAACTG ATTCGTGAAG GGGAGGAGGG ACTTTGGTAT
    GTGAAGAATC TGACCACTGG TAGAGAAGGT TGGATCCCAG CAAACAATCT GCTGATGCTC
    ATAGGCAATT CAAAATCAGC TCAATCTTTG AGCAGCTCTG AATCAGGCAC TGCTTCCAGC
    ACTTTGAGCA CGTCATCAAG TTGCAGTGAT AGCTGCAATG CCAGCAGCAC CAGCTTCTCA
    GACATTAAGG GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30677
    Clone ID Related Accession (Same CDS sequence) XM_025150075.1 , XM_015277853.1 , XM_015277853.2 , XM_015277852.1
    Accession Version XM_025150075.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCATCAGG ATATCATCCC CCTCTATGCA GCAGATATTC ATGACCAGTT AAAGAAACAA 
    TTTGCTTATC TGTCAGGTGG AAGAGGGGGA GATGGATGCC CTGTTATCAC TTTTCCCGAC
    TACCCAGCTT TCAGCGAGAT CCCTGAGAAG GAGTTCCAGA ATGTCCTGAC CTACCTGACC
    AGCATCCCGA GTTTACGAGA TGCTGGAATC GGGTTTATTC TTGTCATAGA CCGCAGACAG
    GACAAATGGA CTTCTGTGAA AGCTTCCATA CTGCGGATAG CAGCATCTTT CCCAGGGAAC
    TTGCAACTCG TCCTCGTTCT GCGCCCAACA GGACTTATTC ATCGAGCTCT CTCAGACATT
    GCTTTCAAGT TCAACAGAGA TGAGTTTAAA ATGAAAGTAC CTATCATAAT GCTGGGCTCA
    GTATCAGAAC TGCAGGGTTA CATTGATAAA ACACAATTAA CAGAGGATCT TGGTGGCACC
    CTGGATTACT GCCATAACAG ATGGCTATCC CTTCGCACTG CTATAGAAGG TTTTGCCCAA
    AAGGTTAAGC AAACAGCTCA GATTCTTCAG TCCTTTGGGA CTGAACTGGC TGAAACAGAA
    CTACCAAATG ATGTGCAGTC AACCAGCTCC CTTCTTGCAA TACATACGGA AAAGAAGGAC
    AAAATGAAGG AAGATATAAG GTTAGCAGTA GAACAGGGAG ACGATATCCT TGGAAGTATT
    ACAGAGCCAG TTACTGAGAA CCCTGAATAC AAGCTGAATC AAGACCAACT AGACAATCAA
    ACTACAGTAG AAAGGCTTTT GGCTCAGTTA CATGAAACAG AGTCCGCCTT TGATGAGTTT
    TGGATTAAGC ACCAGCAAAA ACTTGAGCAG TGTCTGCACC TTCGGAATTT TGAACAGAAT
    TTTAGAGAGG TTAAAGCAGC TTTGGATATT GTGTCCGAGA GACTGTCTGC TTTCACAGAT
    GTGGGAAACA GCTGCTCACA TGTGGAGCAC ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA
    AAATCATGTG AAACTGTAGC AAAAGCCAGA ATGCTAGCTT CAGAGGGTGA TGCTTTTATT
    CAGAGCAATC ATTATGCTGT GGATTCCATT ATTCCAAAAT GCAGTGAGCT TCATCATCTA
    TGTGATGCCT TCACTACTGA GATGGAACAG AGGAGGAATG TTCTTAATAA ATCTCTAGAA
    CTGCATGGCT TACTGGAAAA GTCTATGAAG TGGTGCGATG AAGGAATTTA TCTTCTGGCT
    TCACAACCTG TAGATAAGTG TCAGTCTCAG GATGGTGCAG AATCAGCATT ACAGGAGATT
    GAGAAATTCC TAGACACTGG TGCAGGCAAT AAAATTAAGG AGTTGAATAA AGTTTACAGA
    GAGTATGCAC ACATCCTCAA TGAGGACCTA AAGGAGCATA TACAAAAGGT TTTCCAGAAG
    CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG AGACAAGTAA GTCTTAAGAA ACTGGCAGCT
    AAGCAAACAC GTCCTGTTCA GCCAGTTGCA CCTAGACCCG AAGCCTTTGT AAAATCTCCT
    TGTACCTCTC CAGGTCTTCA AAGAGATTGT GTATCCAACA CAGAAAGCGG TGTGCTTCGG
    AAAGTAAACT ACAGAAGAGG AAAGAGTGAA ATGAGTGAAC TCCATCAAAG CAGAGGAGGA
    TCTACAATGG ATGATGAAGA AAACCTAGCT GTGTTACGGC AGCATGTAAT GAATGAACTT
    ATTGAAACGG AACGAGCATA TGTGGAAGAA CTTCTCTGTG TTCTTGAGGG GTATGCTGCA
    GAAATGGACA ACCCCTTAAT GGCTCACCTC ATTTCACCAG AACTCCAAAA CAAGAAGGAT
    ATTTTGTTTG GGAATATGGA AGAAATTTAT CACTTTCACA ACAGGATATT CTTGAGAGAA
    CTAGAAAATT ATGTAGAATA CCCAGAACTG GTAGGACGGT GCTTTCTGGA TCAGATGGAA
    GATTTCCAGA TTTATGAGAA ATACTGTCAA AACAAACCTC GATCTGAAAG TTTGTGGAGG
    CAATTTTCAG ATTCCATATT CTTTCAGGAA TGTCAAAGGA AACTCGATCA CAAGCTCAGT
    TTGGACTCAT ACCTGCTGAA GCCAGTTCAA AGAATAACCA AATACCAATT ATTGCTAAAG
    GAGATGCTGA AGTACAGTAA GAACTGTGAA GGTGCTGAAG ATCTTCAGGA GGCATTAAAT
    TCTATATTGG GTATTCTCAA AGCAGTCAAT GATTCTATGC ACCAGATAGC CATCACAGGC
    TATGATGGAA ACCTGAATGA GCTGGGCAAG CTCCTAATGC AGGGATCCTT CAACGTCTGG
    ACTGATCATA AAAAGGGTCA CTCAAAGGTG AAAGATTTGG CACGCTTCAA GCCAATGCAG
    CGACACCTGT TCCTCCACGA GAAAGCAGTG CTGTTCTGTA AAAAGCGAGA AGAAAATGGA
    GAAGGATATG AGAAGGCACC TTCTTATAGC TATAAACACT CCTTAAATAT GGCAGCAGTT
    GGAATAACAG AAAATGTCAA AGGTGATGCA AAAAAATTTG AAATCTGGTA TAATGCAAGG
    GAAGAAGTTT ACATCATACA GGCACCAACT CCTGAAGTTA AAGCCACTTG GGTAAATGAA
    ATAAGAAAAG TGCTGACTAG TCAACTGCAG GCATGCAGAG AAGCCAGTCA ACATAGGACA
    CTTGAACACA CTCAGAGTTT ACCTCTACCA GCATCATCCT GTACAAGTCC TTCAAGGAAC
    AACATCAGAA ACGTGAAAAA AATGGATGAG AGAAAAGCTG ATCTCACAAG TCTAGAAGGT
    TATGTCACCA CAGTAGCACC AAAGTACCCC GAGAAAGGCA AAGATGACAC TAACTCTACC
    TCAGAATGCT CTGTACTGTC AAAAAAGCGC TTTACGCTGC AGGGCTTTAC TAACCTCAAA
    AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC AAAAAAGCTA AACGGCACGA AGTAGTGAGT
    GATCCAACTC CTTTTGGTGT AAGAGGTTGG GCTAAAACGT CTCATTCTCT TGATGCATCT
    GAAGAAAATG ATGGCTGGTC CAGTGCTGAG GAGCCGCTGA ATTCATCGGA TGCAGAAGAA
    GAAGGAGGAG GAGGAGGAGA GAGAAATGAG ATGAAGCTGA CTCCTGGTAA ATATACAGTT
    GTGTCTGACT ACGAGAAAGG AGTTTCTGAA GAATTTGCAG TGAAAGGTGG AGATCTAGTT
    CAACTGATTC GTGAAGGGGA GGAGGGACTT TGGTATGTGA AGAATCTGAC CACTGGTAGA
    GAAGGTTGGA TCCCAGCAAA CAATCTGCTG ATGCTCATAG GCAATTCAAA ATCAGCTCAA
    TCTTTGAGCA GCTCTGAATC AGGCACTGCT TCCAGCACTT TGAGCACGTC ATCAAGTTGC
    AGTGATAGCT GCAATGCCAG CAGCACCAGC TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005843.1
    CDS299..3769
    Translation

    Target ORF information:

    RefSeq Version XM_025150075.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025150075.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCATCAGG ATATCATCCC CCTCTATGCA GCAGATATTC ATGACCAGTT AAAGAAACAA 
    TTTGCTTATC TGTCAGGTGG AAGAGGGGGA GATGGATGCC CTGTTATCAC TTTTCCCGAC
    TACCCAGCTT TCAGCGAGAT CCCTGAGAAG GAGTTCCAGA ATGTCCTGAC CTACCTGACC
    AGCATCCCGA GTTTACGAGA TGCTGGAATC GGGTTTATTC TTGTCATAGA CCGCAGACAG
    GACAAATGGA CTTCTGTGAA AGCTTCCATA CTGCGGATAG CAGCATCTTT CCCAGGGAAC
    TTGCAACTCG TCCTCGTTCT GCGCCCAACA GGACTTATTC ATCGAGCTCT CTCAGACATT
    GCTTTCAAGT TCAACAGAGA TGAGTTTAAA ATGAAAGTAC CTATCATAAT GCTGGGCTCA
    GTATCAGAAC TGCAGGGTTA CATTGATAAA ACACAATTAA CAGAGGATCT TGGTGGCACC
    CTGGATTACT GCCATAACAG ATGGCTATCC CTTCGCACTG CTATAGAAGG TTTTGCCCAA
    AAGGTTAAGC AAACAGCTCA GATTCTTCAG TCCTTTGGGA CTGAACTGGC TGAAACAGAA
    CTACCAAATG ATGTGCAGTC AACCAGCTCC CTTCTTGCAA TACATACGGA AAAGAAGGAC
    AAAATGAAGG AAGATATAAG GTTAGCAGTA GAACAGGGAG ACGATATCCT TGGAAGTATT
    ACAGAGCCAG TTACTGAGAA CCCTGAATAC AAGCTGAATC AAGACCAACT AGACAATCAA
    ACTACAGTAG AAAGGCTTTT GGCTCAGTTA CATGAAACAG AGTCCGCCTT TGATGAGTTT
    TGGATTAAGC ACCAGCAAAA ACTTGAGCAG TGTCTGCACC TTCGGAATTT TGAACAGAAT
    TTTAGAGAGG TTAAAGCAGC TTTGGATATT GTGTCCGAGA GACTGTCTGC TTTCACAGAT
    GTGGGAAACA GCTGCTCACA TGTGGAGCAC ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA
    AAATCATGTG AAACTGTAGC AAAAGCCAGA ATGCTAGCTT CAGAGGGTGA TGCTTTTATT
    CAGAGCAATC ATTATGCTGT GGATTCCATT ATTCCAAAAT GCAGTGAGCT TCATCATCTA
    TGTGATGCCT TCACTACTGA GATGGAACAG AGGAGGAATG TTCTTAATAA ATCTCTAGAA
    CTGCATGGCT TACTGGAAAA GTCTATGAAG TGGTGCGATG AAGGAATTTA TCTTCTGGCT
    TCACAACCTG TAGATAAGTG TCAGTCTCAG GATGGTGCAG AATCAGCATT ACAGGAGATT
    GAGAAATTCC TAGACACTGG TGCAGGCAAT AAAATTAAGG AGTTGAATAA AGTTTACAGA
    GAGTATGCAC ACATCCTCAA TGAGGACCTA AAGGAGCATA TACAAAAGGT TTTCCAGAAG
    CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG AGACAAGTAA GTCTTAAGAA ACTGGCAGCT
    AAGCAAACAC GTCCTGTTCA GCCAGTTGCA CCTAGACCCG AAGCCTTTGT AAAATCTCCT
    TGTACCTCTC CAGGTCTTCA AAGAGATTGT GTATCCAACA CAGAAAGCGG TGTGCTTCGG
    AAAGTAAACT ACAGAAGAGG AAAGAGTGAA ATGAGTGAAC TCCATCAAAG CAGAGGAGGA
    TCTACAATGG ATGATGAAGA AAACCTAGCT GTGTTACGGC AGCATGTAAT GAATGAACTT
    ATTGAAACGG AACGAGCATA TGTGGAAGAA CTTCTCTGTG TTCTTGAGGG GTATGCTGCA
    GAAATGGACA ACCCCTTAAT GGCTCACCTC ATTTCACCAG AACTCCAAAA CAAGAAGGAT
    ATTTTGTTTG GGAATATGGA AGAAATTTAT CACTTTCACA ACAGGATATT CTTGAGAGAA
    CTAGAAAATT ATGTAGAATA CCCAGAACTG GTAGGACGGT GCTTTCTGGA TCAGATGGAA
    GATTTCCAGA TTTATGAGAA ATACTGTCAA AACAAACCTC GATCTGAAAG TTTGTGGAGG
    CAATTTTCAG ATTCCATATT CTTTCAGGAA TGTCAAAGGA AACTCGATCA CAAGCTCAGT
    TTGGACTCAT ACCTGCTGAA GCCAGTTCAA AGAATAACCA AATACCAATT ATTGCTAAAG
    GAGATGCTGA AGTACAGTAA GAACTGTGAA GGTGCTGAAG ATCTTCAGGA GGCATTAAAT
    TCTATATTGG GTATTCTCAA AGCAGTCAAT GATTCTATGC ACCAGATAGC CATCACAGGC
    TATGATGGAA ACCTGAATGA GCTGGGCAAG CTCCTAATGC AGGGATCCTT CAACGTCTGG
    ACTGATCATA AAAAGGGTCA CTCAAAGGTG AAAGATTTGG CACGCTTCAA GCCAATGCAG
    CGACACCTGT TCCTCCACGA GAAAGCAGTG CTGTTCTGTA AAAAGCGAGA AGAAAATGGA
    GAAGGATATG AGAAGGCACC TTCTTATAGC TATAAACACT CCTTAAATAT GGCAGCAGTT
    GGAATAACAG AAAATGTCAA AGGTGATGCA AAAAAATTTG AAATCTGGTA TAATGCAAGG
    GAAGAAGTTT ACATCATACA GGCACCAACT CCTGAAGTTA AAGCCACTTG GGTAAATGAA
    ATAAGAAAAG TGCTGACTAG TCAACTGCAG GCATGCAGAG AAGCCAGTCA ACATAGGACA
    CTTGAACACA CTCAGAGTTT ACCTCTACCA GCATCATCCT GTACAAGTCC TTCAAGGAAC
    AACATCAGAA ACGTGAAAAA AATGGATGAG AGAAAAGCTG ATCTCACAAG TCTAGAAGGT
    TATGTCACCA CAGTAGCACC AAAGTACCCC GAGAAAGGCA AAGATGACAC TAACTCTACC
    TCAGAATGCT CTGTACTGTC AAAAAAGCGC TTTACGCTGC AGGGCTTTAC TAACCTCAAA
    AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC AAAAAAGCTA AACGGCACGA AGTAGTGAGT
    GATCCAACTC CTTTTGGTGT AAGAGGTTGG GCTAAAACGT CTCATTCTCT TGATGCATCT
    GAAGAAAATG ATGGCTGGTC CAGTGCTGAG GAGCCGCTGA ATTCATCGGA TGCAGAAGAA
    GAAGGAGGAG GAGGAGGAGA GAGAAATGAG ATGAAGCTGA CTCCTGGTAA ATATACAGTT
    GTGTCTGACT ACGAGAAAGG AGTTTCTGAA GAATTTGCAG TGAAAGGTGG AGATCTAGTT
    CAACTGATTC GTGAAGGGGA GGAGGGACTT TGGTATGTGA AGAATCTGAC CACTGGTAGA
    GAAGGTTGGA TCCCAGCAAA CAATCTGCTG ATGCTCATAG GCAATTCAAA ATCAGCTCAA
    TCTTTGAGCA GCTCTGAATC AGGCACTGCT TCCAGCACTT TGAGCACGTC ATCAAGTTGC
    AGTGATAGCT GCAATGCCAG CAGCACCAGC TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30677
    Clone ID Related Accession (Same CDS sequence) XM_025150075.1 , XM_015277853.1 , XM_015277853.2 , XM_015277852.1
    Accession Version XM_015277853.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCATCAGG ATATCATCCC CCTCTATGCA GCAGATATTC ATGACCAGTT AAAGAAACAA 
    TTTGCTTATC TGTCAGGTGG AAGAGGGGGA GATGGATGCC CTGTTATCAC TTTTCCCGAC
    TACCCAGCTT TCAGCGAGAT CCCTGAGAAG GAGTTCCAGA ATGTCCTGAC CTACCTGACC
    AGCATCCCGA GTTTACGAGA TGCTGGAATC GGGTTTATTC TTGTCATAGA CCGCAGACAG
    GACAAATGGA CTTCTGTGAA AGCTTCCATA CTGCGGATAG CAGCATCTTT CCCAGGGAAC
    TTGCAACTCG TCCTCGTTCT GCGCCCAACA GGACTTATTC ATCGAGCTCT CTCAGACATT
    GCTTTCAAGT TCAACAGAGA TGAGTTTAAA ATGAAAGTAC CTATCATAAT GCTGGGCTCA
    GTATCAGAAC TGCAGGGTTA CATTGATAAA ACACAATTAA CAGAGGATCT TGGTGGCACC
    CTGGATTACT GCCATAACAG ATGGCTATCC CTTCGCACTG CTATAGAAGG TTTTGCCCAA
    AAGGTTAAGC AAACAGCTCA GATTCTTCAG TCCTTTGGGA CTGAACTGGC TGAAACAGAA
    CTACCAAATG ATGTGCAGTC AACCAGCTCC CTTCTTGCAA TACATACGGA AAAGAAGGAC
    AAAATGAAGG AAGATATAAG GTTAGCAGTA GAACAGGGAG ACGATATCCT TGGAAGTATT
    ACAGAGCCAG TTACTGAGAA CCCTGAATAC AAGCTGAATC AAGACCAACT AGACAATCAA
    ACTACAGTAG AAAGGCTTTT GGCTCAGTTA CATGAAACAG AGTCCGCCTT TGATGAGTTT
    TGGATTAAGC ACCAGCAAAA ACTTGAGCAG TGTCTGCACC TTCGGAATTT TGAACAGAAT
    TTTAGAGAGG TTAAAGCAGC TTTGGATATT GTGTCCGAGA GACTGTCTGC TTTCACAGAT
    GTGGGAAACA GCTGCTCACA TGTGGAGCAC ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA
    AAATCATGTG AAACTGTAGC AAAAGCCAGA ATGCTAGCTT CAGAGGGTGA TGCTTTTATT
    CAGAGCAATC ATTATGCTGT GGATTCCATT ATTCCAAAAT GCAGTGAGCT TCATCATCTA
    TGTGATGCCT TCACTACTGA GATGGAACAG AGGAGGAATG TTCTTAATAA ATCTCTAGAA
    CTGCATGGCT TACTGGAAAA GTCTATGAAG TGGTGCGATG AAGGAATTTA TCTTCTGGCT
    TCACAACCTG TAGATAAGTG TCAGTCTCAG GATGGTGCAG AATCAGCATT ACAGGAGATT
    GAGAAATTCC TAGACACTGG TGCAGGCAAT AAAATTAAGG AGTTGAATAA AGTTTACAGA
    GAGTATGCAC ACATCCTCAA TGAGGACCTA AAGGAGCATA TACAAAAGGT TTTCCAGAAG
    CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG AGACAAGTAA GTCTTAAGAA ACTGGCAGCT
    AAGCAAACAC GTCCTGTTCA GCCAGTTGCA CCTAGACCCG AAGCCTTTGT AAAATCTCCT
    TGTACCTCTC CAGGTCTTCA AAGAGATTGT GTATCCAACA CAGAAAGCGG TGTGCTTCGG
    AAAGTAAACT ACAGAAGAGG AAAGAGTGAA ATGAGTGAAC TCCATCAAAG CAGAGGAGGA
    TCTACAATGG ATGATGAAGA AAACCTAGCT GTGTTACGGC AGCATGTAAT GAATGAACTT
    ATTGAAACGG AACGAGCATA TGTGGAAGAA CTTCTCTGTG TTCTTGAGGG GTATGCTGCA
    GAAATGGACA ACCCCTTAAT GGCTCACCTC ATTTCACCAG AACTCCAAAA CAAGAAGGAT
    ATTTTGTTTG GGAATATGGA AGAAATTTAT CACTTTCACA ACAGGATATT CTTGAGAGAA
    CTAGAAAATT ATGTAGAATA CCCAGAACTG GTAGGACGGT GCTTTCTGGA TCAGATGGAA
    GATTTCCAGA TTTATGAGAA ATACTGTCAA AACAAACCTC GATCTGAAAG TTTGTGGAGG
    CAATTTTCAG ATTCCATATT CTTTCAGGAA TGTCAAAGGA AACTCGATCA CAAGCTCAGT
    TTGGACTCAT ACCTGCTGAA GCCAGTTCAA AGAATAACCA AATACCAATT ATTGCTAAAG
    GAGATGCTGA AGTACAGTAA GAACTGTGAA GGTGCTGAAG ATCTTCAGGA GGCATTAAAT
    TCTATATTGG GTATTCTCAA AGCAGTCAAT GATTCTATGC ACCAGATAGC CATCACAGGC
    TATGATGGAA ACCTGAATGA GCTGGGCAAG CTCCTAATGC AGGGATCCTT CAACGTCTGG
    ACTGATCATA AAAAGGGTCA CTCAAAGGTG AAAGATTTGG CACGCTTCAA GCCAATGCAG
    CGACACCTGT TCCTCCACGA GAAAGCAGTG CTGTTCTGTA AAAAGCGAGA AGAAAATGGA
    GAAGGATATG AGAAGGCACC TTCTTATAGC TATAAACACT CCTTAAATAT GGCAGCAGTT
    GGAATAACAG AAAATGTCAA AGGTGATGCA AAAAAATTTG AAATCTGGTA TAATGCAAGG
    GAAGAAGTTT ACATCATACA GGCACCAACT CCTGAAGTTA AAGCCACTTG GGTAAATGAA
    ATAAGAAAAG TGCTGACTAG TCAACTGCAG GCATGCAGAG AAGCCAGTCA ACATAGGACA
    CTTGAACACA CTCAGAGTTT ACCTCTACCA GCATCATCCT GTACAAGTCC TTCAAGGAAC
    AACATCAGAA ACGTGAAAAA AATGGATGAG AGAAAAGCTG ATCTCACAAG TCTAGAAGGT
    TATGTCACCA CAGTAGCACC AAAGTACCCC GAGAAAGGCA AAGATGACAC TAACTCTACC
    TCAGAATGCT CTGTACTGTC AAAAAAGCGC TTTACGCTGC AGGGCTTTAC TAACCTCAAA
    AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC AAAAAAGCTA AACGGCACGA AGTAGTGAGT
    GATCCAACTC CTTTTGGTGT AAGAGGTTGG GCTAAAACGT CTCATTCTCT TGATGCATCT
    GAAGAAAATG ATGGCTGGTC CAGTGCTGAG GAGCCGCTGA ATTCATCGGA TGCAGAAGAA
    GAAGGAGGAG GAGGAGGAGA GAGAAATGAG ATGAAGCTGA CTCCTGGTAA ATATACAGTT
    GTGTCTGACT ACGAGAAAGG AGTTTCTGAA GAATTTGCAG TGAAAGGTGG AGATCTAGTT
    CAACTGATTC GTGAAGGGGA GGAGGGACTT TGGTATGTGA AGAATCTGAC CACTGGTAGA
    GAAGGTTGGA TCCCAGCAAA CAATCTGCTG ATGCTCATAG GCAATTCAAA ATCAGCTCAA
    TCTTTGAGCA GCTCTGAATC AGGCACTGCT TCCAGCACTT TGAGCACGTC ATCAAGTTGC
    AGTGATAGCT GCAATGCCAG CAGCACCAGC TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133339.1
    CDS97..3567
    Translation

    Target ORF information:

    RefSeq Version XM_015277853.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277853.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCATCAGG ATATCATCCC CCTCTATGCA GCAGATATTC ATGACCAGTT AAAGAAACAA 
    TTTGCTTATC TGTCAGGTGG AAGAGGGGGA GATGGATGCC CTGTTATCAC TTTTCCCGAC
    TACCCAGCTT TCAGCGAGAT CCCTGAGAAG GAGTTCCAGA ATGTCCTGAC CTACCTGACC
    AGCATCCCGA GTTTACGAGA TGCTGGAATC GGGTTTATTC TTGTCATAGA CCGCAGACAG
    GACAAATGGA CTTCTGTGAA AGCTTCCATA CTGCGGATAG CAGCATCTTT CCCAGGGAAC
    TTGCAACTCG TCCTCGTTCT GCGCCCAACA GGACTTATTC ATCGAGCTCT CTCAGACATT
    GCTTTCAAGT TCAACAGAGA TGAGTTTAAA ATGAAAGTAC CTATCATAAT GCTGGGCTCA
    GTATCAGAAC TGCAGGGTTA CATTGATAAA ACACAATTAA CAGAGGATCT TGGTGGCACC
    CTGGATTACT GCCATAACAG ATGGCTATCC CTTCGCACTG CTATAGAAGG TTTTGCCCAA
    AAGGTTAAGC AAACAGCTCA GATTCTTCAG TCCTTTGGGA CTGAACTGGC TGAAACAGAA
    CTACCAAATG ATGTGCAGTC AACCAGCTCC CTTCTTGCAA TACATACGGA AAAGAAGGAC
    AAAATGAAGG AAGATATAAG GTTAGCAGTA GAACAGGGAG ACGATATCCT TGGAAGTATT
    ACAGAGCCAG TTACTGAGAA CCCTGAATAC AAGCTGAATC AAGACCAACT AGACAATCAA
    ACTACAGTAG AAAGGCTTTT GGCTCAGTTA CATGAAACAG AGTCCGCCTT TGATGAGTTT
    TGGATTAAGC ACCAGCAAAA ACTTGAGCAG TGTCTGCACC TTCGGAATTT TGAACAGAAT
    TTTAGAGAGG TTAAAGCAGC TTTGGATATT GTGTCCGAGA GACTGTCTGC TTTCACAGAT
    GTGGGAAACA GCTGCTCACA TGTGGAGCAC ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA
    AAATCATGTG AAACTGTAGC AAAAGCCAGA ATGCTAGCTT CAGAGGGTGA TGCTTTTATT
    CAGAGCAATC ATTATGCTGT GGATTCCATT ATTCCAAAAT GCAGTGAGCT TCATCATCTA
    TGTGATGCCT TCACTACTGA GATGGAACAG AGGAGGAATG TTCTTAATAA ATCTCTAGAA
    CTGCATGGCT TACTGGAAAA GTCTATGAAG TGGTGCGATG AAGGAATTTA TCTTCTGGCT
    TCACAACCTG TAGATAAGTG TCAGTCTCAG GATGGTGCAG AATCAGCATT ACAGGAGATT
    GAGAAATTCC TAGACACTGG TGCAGGCAAT AAAATTAAGG AGTTGAATAA AGTTTACAGA
    GAGTATGCAC ACATCCTCAA TGAGGACCTA AAGGAGCATA TACAAAAGGT TTTCCAGAAG
    CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG AGACAAGTAA GTCTTAAGAA ACTGGCAGCT
    AAGCAAACAC GTCCTGTTCA GCCAGTTGCA CCTAGACCCG AAGCCTTTGT AAAATCTCCT
    TGTACCTCTC CAGGTCTTCA AAGAGATTGT GTATCCAACA CAGAAAGCGG TGTGCTTCGG
    AAAGTAAACT ACAGAAGAGG AAAGAGTGAA ATGAGTGAAC TCCATCAAAG CAGAGGAGGA
    TCTACAATGG ATGATGAAGA AAACCTAGCT GTGTTACGGC AGCATGTAAT GAATGAACTT
    ATTGAAACGG AACGAGCATA TGTGGAAGAA CTTCTCTGTG TTCTTGAGGG GTATGCTGCA
    GAAATGGACA ACCCCTTAAT GGCTCACCTC ATTTCACCAG AACTCCAAAA CAAGAAGGAT
    ATTTTGTTTG GGAATATGGA AGAAATTTAT CACTTTCACA ACAGGATATT CTTGAGAGAA
    CTAGAAAATT ATGTAGAATA CCCAGAACTG GTAGGACGGT GCTTTCTGGA TCAGATGGAA
    GATTTCCAGA TTTATGAGAA ATACTGTCAA AACAAACCTC GATCTGAAAG TTTGTGGAGG
    CAATTTTCAG ATTCCATATT CTTTCAGGAA TGTCAAAGGA AACTCGATCA CAAGCTCAGT
    TTGGACTCAT ACCTGCTGAA GCCAGTTCAA AGAATAACCA AATACCAATT ATTGCTAAAG
    GAGATGCTGA AGTACAGTAA GAACTGTGAA GGTGCTGAAG ATCTTCAGGA GGCATTAAAT
    TCTATATTGG GTATTCTCAA AGCAGTCAAT GATTCTATGC ACCAGATAGC CATCACAGGC
    TATGATGGAA ACCTGAATGA GCTGGGCAAG CTCCTAATGC AGGGATCCTT CAACGTCTGG
    ACTGATCATA AAAAGGGTCA CTCAAAGGTG AAAGATTTGG CACGCTTCAA GCCAATGCAG
    CGACACCTGT TCCTCCACGA GAAAGCAGTG CTGTTCTGTA AAAAGCGAGA AGAAAATGGA
    GAAGGATATG AGAAGGCACC TTCTTATAGC TATAAACACT CCTTAAATAT GGCAGCAGTT
    GGAATAACAG AAAATGTCAA AGGTGATGCA AAAAAATTTG AAATCTGGTA TAATGCAAGG
    GAAGAAGTTT ACATCATACA GGCACCAACT CCTGAAGTTA AAGCCACTTG GGTAAATGAA
    ATAAGAAAAG TGCTGACTAG TCAACTGCAG GCATGCAGAG AAGCCAGTCA ACATAGGACA
    CTTGAACACA CTCAGAGTTT ACCTCTACCA GCATCATCCT GTACAAGTCC TTCAAGGAAC
    AACATCAGAA ACGTGAAAAA AATGGATGAG AGAAAAGCTG ATCTCACAAG TCTAGAAGGT
    TATGTCACCA CAGTAGCACC AAAGTACCCC GAGAAAGGCA AAGATGACAC TAACTCTACC
    TCAGAATGCT CTGTACTGTC AAAAAAGCGC TTTACGCTGC AGGGCTTTAC TAACCTCAAA
    AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC AAAAAAGCTA AACGGCACGA AGTAGTGAGT
    GATCCAACTC CTTTTGGTGT AAGAGGTTGG GCTAAAACGT CTCATTCTCT TGATGCATCT
    GAAGAAAATG ATGGCTGGTC CAGTGCTGAG GAGCCGCTGA ATTCATCGGA TGCAGAAGAA
    GAAGGAGGAG GAGGAGGAGA GAGAAATGAG ATGAAGCTGA CTCCTGGTAA ATATACAGTT
    GTGTCTGACT ACGAGAAAGG AGTTTCTGAA GAATTTGCAG TGAAAGGTGG AGATCTAGTT
    CAACTGATTC GTGAAGGGGA GGAGGGACTT TGGTATGTGA AGAATCTGAC CACTGGTAGA
    GAAGGTTGGA TCCCAGCAAA CAATCTGCTG ATGCTCATAG GCAATTCAAA ATCAGCTCAA
    TCTTTGAGCA GCTCTGAATC AGGCACTGCT TCCAGCACTT TGAGCACGTC ATCAAGTTGC
    AGTGATAGCT GCAATGCCAG CAGCACCAGC TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30677
    Clone ID Related Accession (Same CDS sequence) XM_025150075.1 , XM_015277853.1 , XM_015277853.2 , XM_015277852.1
    Accession Version XM_015277853.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCATCAGG ATATCATCCC CCTCTATGCA GCAGATATTC ATGACCAGTT AAAGAAACAA 
    TTTGCTTATC TGTCAGGTGG AAGAGGGGGA GATGGATGCC CTGTTATCAC TTTTCCCGAC
    TACCCAGCTT TCAGCGAGAT CCCTGAGAAG GAGTTCCAGA ATGTCCTGAC CTACCTGACC
    AGCATCCCGA GTTTACGAGA TGCTGGAATC GGGTTTATTC TTGTCATAGA CCGCAGACAG
    GACAAATGGA CTTCTGTGAA AGCTTCCATA CTGCGGATAG CAGCATCTTT CCCAGGGAAC
    TTGCAACTCG TCCTCGTTCT GCGCCCAACA GGACTTATTC ATCGAGCTCT CTCAGACATT
    GCTTTCAAGT TCAACAGAGA TGAGTTTAAA ATGAAAGTAC CTATCATAAT GCTGGGCTCA
    GTATCAGAAC TGCAGGGTTA CATTGATAAA ACACAATTAA CAGAGGATCT TGGTGGCACC
    CTGGATTACT GCCATAACAG ATGGCTATCC CTTCGCACTG CTATAGAAGG TTTTGCCCAA
    AAGGTTAAGC AAACAGCTCA GATTCTTCAG TCCTTTGGGA CTGAACTGGC TGAAACAGAA
    CTACCAAATG ATGTGCAGTC AACCAGCTCC CTTCTTGCAA TACATACGGA AAAGAAGGAC
    AAAATGAAGG AAGATATAAG GTTAGCAGTA GAACAGGGAG ACGATATCCT TGGAAGTATT
    ACAGAGCCAG TTACTGAGAA CCCTGAATAC AAGCTGAATC AAGACCAACT AGACAATCAA
    ACTACAGTAG AAAGGCTTTT GGCTCAGTTA CATGAAACAG AGTCCGCCTT TGATGAGTTT
    TGGATTAAGC ACCAGCAAAA ACTTGAGCAG TGTCTGCACC TTCGGAATTT TGAACAGAAT
    TTTAGAGAGG TTAAAGCAGC TTTGGATATT GTGTCCGAGA GACTGTCTGC TTTCACAGAT
    GTGGGAAACA GCTGCTCACA TGTGGAGCAC ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA
    AAATCATGTG AAACTGTAGC AAAAGCCAGA ATGCTAGCTT CAGAGGGTGA TGCTTTTATT
    CAGAGCAATC ATTATGCTGT GGATTCCATT ATTCCAAAAT GCAGTGAGCT TCATCATCTA
    TGTGATGCCT TCACTACTGA GATGGAACAG AGGAGGAATG TTCTTAATAA ATCTCTAGAA
    CTGCATGGCT TACTGGAAAA GTCTATGAAG TGGTGCGATG AAGGAATTTA TCTTCTGGCT
    TCACAACCTG TAGATAAGTG TCAGTCTCAG GATGGTGCAG AATCAGCATT ACAGGAGATT
    GAGAAATTCC TAGACACTGG TGCAGGCAAT AAAATTAAGG AGTTGAATAA AGTTTACAGA
    GAGTATGCAC ACATCCTCAA TGAGGACCTA AAGGAGCATA TACAAAAGGT TTTCCAGAAG
    CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG AGACAAGTAA GTCTTAAGAA ACTGGCAGCT
    AAGCAAACAC GTCCTGTTCA GCCAGTTGCA CCTAGACCCG AAGCCTTTGT AAAATCTCCT
    TGTACCTCTC CAGGTCTTCA AAGAGATTGT GTATCCAACA CAGAAAGCGG TGTGCTTCGG
    AAAGTAAACT ACAGAAGAGG AAAGAGTGAA ATGAGTGAAC TCCATCAAAG CAGAGGAGGA
    TCTACAATGG ATGATGAAGA AAACCTAGCT GTGTTACGGC AGCATGTAAT GAATGAACTT
    ATTGAAACGG AACGAGCATA TGTGGAAGAA CTTCTCTGTG TTCTTGAGGG GTATGCTGCA
    GAAATGGACA ACCCCTTAAT GGCTCACCTC ATTTCACCAG AACTCCAAAA CAAGAAGGAT
    ATTTTGTTTG GGAATATGGA AGAAATTTAT CACTTTCACA ACAGGATATT CTTGAGAGAA
    CTAGAAAATT ATGTAGAATA CCCAGAACTG GTAGGACGGT GCTTTCTGGA TCAGATGGAA
    GATTTCCAGA TTTATGAGAA ATACTGTCAA AACAAACCTC GATCTGAAAG TTTGTGGAGG
    CAATTTTCAG ATTCCATATT CTTTCAGGAA TGTCAAAGGA AACTCGATCA CAAGCTCAGT
    TTGGACTCAT ACCTGCTGAA GCCAGTTCAA AGAATAACCA AATACCAATT ATTGCTAAAG
    GAGATGCTGA AGTACAGTAA GAACTGTGAA GGTGCTGAAG ATCTTCAGGA GGCATTAAAT
    TCTATATTGG GTATTCTCAA AGCAGTCAAT GATTCTATGC ACCAGATAGC CATCACAGGC
    TATGATGGAA ACCTGAATGA GCTGGGCAAG CTCCTAATGC AGGGATCCTT CAACGTCTGG
    ACTGATCATA AAAAGGGTCA CTCAAAGGTG AAAGATTTGG CACGCTTCAA GCCAATGCAG
    CGACACCTGT TCCTCCACGA GAAAGCAGTG CTGTTCTGTA AAAAGCGAGA AGAAAATGGA
    GAAGGATATG AGAAGGCACC TTCTTATAGC TATAAACACT CCTTAAATAT GGCAGCAGTT
    GGAATAACAG AAAATGTCAA AGGTGATGCA AAAAAATTTG AAATCTGGTA TAATGCAAGG
    GAAGAAGTTT ACATCATACA GGCACCAACT CCTGAAGTTA AAGCCACTTG GGTAAATGAA
    ATAAGAAAAG TGCTGACTAG TCAACTGCAG GCATGCAGAG AAGCCAGTCA ACATAGGACA
    CTTGAACACA CTCAGAGTTT ACCTCTACCA GCATCATCCT GTACAAGTCC TTCAAGGAAC
    AACATCAGAA ACGTGAAAAA AATGGATGAG AGAAAAGCTG ATCTCACAAG TCTAGAAGGT
    TATGTCACCA CAGTAGCACC AAAGTACCCC GAGAAAGGCA AAGATGACAC TAACTCTACC
    TCAGAATGCT CTGTACTGTC AAAAAAGCGC TTTACGCTGC AGGGCTTTAC TAACCTCAAA
    AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC AAAAAAGCTA AACGGCACGA AGTAGTGAGT
    GATCCAACTC CTTTTGGTGT AAGAGGTTGG GCTAAAACGT CTCATTCTCT TGATGCATCT
    GAAGAAAATG ATGGCTGGTC CAGTGCTGAG GAGCCGCTGA ATTCATCGGA TGCAGAAGAA
    GAAGGAGGAG GAGGAGGAGA GAGAAATGAG ATGAAGCTGA CTCCTGGTAA ATATACAGTT
    GTGTCTGACT ACGAGAAAGG AGTTTCTGAA GAATTTGCAG TGAAAGGTGG AGATCTAGTT
    CAACTGATTC GTGAAGGGGA GGAGGGACTT TGGTATGTGA AGAATCTGAC CACTGGTAGA
    GAAGGTTGGA TCCCAGCAAA CAATCTGCTG ATGCTCATAG GCAATTCAAA ATCAGCTCAA
    TCTTTGAGCA GCTCTGAATC AGGCACTGCT TCCAGCACTT TGAGCACGTC ATCAAGTTGC
    AGTGATAGCT GCAATGCCAG CAGCACCAGC TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133339.1
    CDS76..3546
    Translation

    Target ORF information:

    RefSeq Version XM_015277853.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277853.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCATCAGG ATATCATCCC CCTCTATGCA GCAGATATTC ATGACCAGTT AAAGAAACAA 
    TTTGCTTATC TGTCAGGTGG AAGAGGGGGA GATGGATGCC CTGTTATCAC TTTTCCCGAC
    TACCCAGCTT TCAGCGAGAT CCCTGAGAAG GAGTTCCAGA ATGTCCTGAC CTACCTGACC
    AGCATCCCGA GTTTACGAGA TGCTGGAATC GGGTTTATTC TTGTCATAGA CCGCAGACAG
    GACAAATGGA CTTCTGTGAA AGCTTCCATA CTGCGGATAG CAGCATCTTT CCCAGGGAAC
    TTGCAACTCG TCCTCGTTCT GCGCCCAACA GGACTTATTC ATCGAGCTCT CTCAGACATT
    GCTTTCAAGT TCAACAGAGA TGAGTTTAAA ATGAAAGTAC CTATCATAAT GCTGGGCTCA
    GTATCAGAAC TGCAGGGTTA CATTGATAAA ACACAATTAA CAGAGGATCT TGGTGGCACC
    CTGGATTACT GCCATAACAG ATGGCTATCC CTTCGCACTG CTATAGAAGG TTTTGCCCAA
    AAGGTTAAGC AAACAGCTCA GATTCTTCAG TCCTTTGGGA CTGAACTGGC TGAAACAGAA
    CTACCAAATG ATGTGCAGTC AACCAGCTCC CTTCTTGCAA TACATACGGA AAAGAAGGAC
    AAAATGAAGG AAGATATAAG GTTAGCAGTA GAACAGGGAG ACGATATCCT TGGAAGTATT
    ACAGAGCCAG TTACTGAGAA CCCTGAATAC AAGCTGAATC AAGACCAACT AGACAATCAA
    ACTACAGTAG AAAGGCTTTT GGCTCAGTTA CATGAAACAG AGTCCGCCTT TGATGAGTTT
    TGGATTAAGC ACCAGCAAAA ACTTGAGCAG TGTCTGCACC TTCGGAATTT TGAACAGAAT
    TTTAGAGAGG TTAAAGCAGC TTTGGATATT GTGTCCGAGA GACTGTCTGC TTTCACAGAT
    GTGGGAAACA GCTGCTCACA TGTGGAGCAC ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA
    AAATCATGTG AAACTGTAGC AAAAGCCAGA ATGCTAGCTT CAGAGGGTGA TGCTTTTATT
    CAGAGCAATC ATTATGCTGT GGATTCCATT ATTCCAAAAT GCAGTGAGCT TCATCATCTA
    TGTGATGCCT TCACTACTGA GATGGAACAG AGGAGGAATG TTCTTAATAA ATCTCTAGAA
    CTGCATGGCT TACTGGAAAA GTCTATGAAG TGGTGCGATG AAGGAATTTA TCTTCTGGCT
    TCACAACCTG TAGATAAGTG TCAGTCTCAG GATGGTGCAG AATCAGCATT ACAGGAGATT
    GAGAAATTCC TAGACACTGG TGCAGGCAAT AAAATTAAGG AGTTGAATAA AGTTTACAGA
    GAGTATGCAC ACATCCTCAA TGAGGACCTA AAGGAGCATA TACAAAAGGT TTTCCAGAAG
    CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG AGACAAGTAA GTCTTAAGAA ACTGGCAGCT
    AAGCAAACAC GTCCTGTTCA GCCAGTTGCA CCTAGACCCG AAGCCTTTGT AAAATCTCCT
    TGTACCTCTC CAGGTCTTCA AAGAGATTGT GTATCCAACA CAGAAAGCGG TGTGCTTCGG
    AAAGTAAACT ACAGAAGAGG AAAGAGTGAA ATGAGTGAAC TCCATCAAAG CAGAGGAGGA
    TCTACAATGG ATGATGAAGA AAACCTAGCT GTGTTACGGC AGCATGTAAT GAATGAACTT
    ATTGAAACGG AACGAGCATA TGTGGAAGAA CTTCTCTGTG TTCTTGAGGG GTATGCTGCA
    GAAATGGACA ACCCCTTAAT GGCTCACCTC ATTTCACCAG AACTCCAAAA CAAGAAGGAT
    ATTTTGTTTG GGAATATGGA AGAAATTTAT CACTTTCACA ACAGGATATT CTTGAGAGAA
    CTAGAAAATT ATGTAGAATA CCCAGAACTG GTAGGACGGT GCTTTCTGGA TCAGATGGAA
    GATTTCCAGA TTTATGAGAA ATACTGTCAA AACAAACCTC GATCTGAAAG TTTGTGGAGG
    CAATTTTCAG ATTCCATATT CTTTCAGGAA TGTCAAAGGA AACTCGATCA CAAGCTCAGT
    TTGGACTCAT ACCTGCTGAA GCCAGTTCAA AGAATAACCA AATACCAATT ATTGCTAAAG
    GAGATGCTGA AGTACAGTAA GAACTGTGAA GGTGCTGAAG ATCTTCAGGA GGCATTAAAT
    TCTATATTGG GTATTCTCAA AGCAGTCAAT GATTCTATGC ACCAGATAGC CATCACAGGC
    TATGATGGAA ACCTGAATGA GCTGGGCAAG CTCCTAATGC AGGGATCCTT CAACGTCTGG
    ACTGATCATA AAAAGGGTCA CTCAAAGGTG AAAGATTTGG CACGCTTCAA GCCAATGCAG
    CGACACCTGT TCCTCCACGA GAAAGCAGTG CTGTTCTGTA AAAAGCGAGA AGAAAATGGA
    GAAGGATATG AGAAGGCACC TTCTTATAGC TATAAACACT CCTTAAATAT GGCAGCAGTT
    GGAATAACAG AAAATGTCAA AGGTGATGCA AAAAAATTTG AAATCTGGTA TAATGCAAGG
    GAAGAAGTTT ACATCATACA GGCACCAACT CCTGAAGTTA AAGCCACTTG GGTAAATGAA
    ATAAGAAAAG TGCTGACTAG TCAACTGCAG GCATGCAGAG AAGCCAGTCA ACATAGGACA
    CTTGAACACA CTCAGAGTTT ACCTCTACCA GCATCATCCT GTACAAGTCC TTCAAGGAAC
    AACATCAGAA ACGTGAAAAA AATGGATGAG AGAAAAGCTG ATCTCACAAG TCTAGAAGGT
    TATGTCACCA CAGTAGCACC AAAGTACCCC GAGAAAGGCA AAGATGACAC TAACTCTACC
    TCAGAATGCT CTGTACTGTC AAAAAAGCGC TTTACGCTGC AGGGCTTTAC TAACCTCAAA
    AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC AAAAAAGCTA AACGGCACGA AGTAGTGAGT
    GATCCAACTC CTTTTGGTGT AAGAGGTTGG GCTAAAACGT CTCATTCTCT TGATGCATCT
    GAAGAAAATG ATGGCTGGTC CAGTGCTGAG GAGCCGCTGA ATTCATCGGA TGCAGAAGAA
    GAAGGAGGAG GAGGAGGAGA GAGAAATGAG ATGAAGCTGA CTCCTGGTAA ATATACAGTT
    GTGTCTGACT ACGAGAAAGG AGTTTCTGAA GAATTTGCAG TGAAAGGTGG AGATCTAGTT
    CAACTGATTC GTGAAGGGGA GGAGGGACTT TGGTATGTGA AGAATCTGAC CACTGGTAGA
    GAAGGTTGGA TCCCAGCAAA CAATCTGCTG ATGCTCATAG GCAATTCAAA ATCAGCTCAA
    TCTTTGAGCA GCTCTGAATC AGGCACTGCT TCCAGCACTT TGAGCACGTC ATCAAGTTGC
    AGTGATAGCT GCAATGCCAG CAGCACCAGC TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30677
    Clone ID Related Accession (Same CDS sequence) XM_025150075.1 , XM_015277853.1 , XM_015277853.2 , XM_015277852.1
    Accession Version XM_015277852.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCATCAGG ATATCATCCC CCTCTATGCA GCAGATATTC ATGACCAGTT AAAGAAACAA 
    TTTGCTTATC TGTCAGGTGG AAGAGGGGGA GATGGATGCC CTGTTATCAC TTTTCCCGAC
    TACCCAGCTT TCAGCGAGAT CCCTGAGAAG GAGTTCCAGA ATGTCCTGAC CTACCTGACC
    AGCATCCCGA GTTTACGAGA TGCTGGAATC GGGTTTATTC TTGTCATAGA CCGCAGACAG
    GACAAATGGA CTTCTGTGAA AGCTTCCATA CTGCGGATAG CAGCATCTTT CCCAGGGAAC
    TTGCAACTCG TCCTCGTTCT GCGCCCAACA GGACTTATTC ATCGAGCTCT CTCAGACATT
    GCTTTCAAGT TCAACAGAGA TGAGTTTAAA ATGAAAGTAC CTATCATAAT GCTGGGCTCA
    GTATCAGAAC TGCAGGGTTA CATTGATAAA ACACAATTAA CAGAGGATCT TGGTGGCACC
    CTGGATTACT GCCATAACAG ATGGCTATCC CTTCGCACTG CTATAGAAGG TTTTGCCCAA
    AAGGTTAAGC AAACAGCTCA GATTCTTCAG TCCTTTGGGA CTGAACTGGC TGAAACAGAA
    CTACCAAATG ATGTGCAGTC AACCAGCTCC CTTCTTGCAA TACATACGGA AAAGAAGGAC
    AAAATGAAGG AAGATATAAG GTTAGCAGTA GAACAGGGAG ACGATATCCT TGGAAGTATT
    ACAGAGCCAG TTACTGAGAA CCCTGAATAC AAGCTGAATC AAGACCAACT AGACAATCAA
    ACTACAGTAG AAAGGCTTTT GGCTCAGTTA CATGAAACAG AGTCCGCCTT TGATGAGTTT
    TGGATTAAGC ACCAGCAAAA ACTTGAGCAG TGTCTGCACC TTCGGAATTT TGAACAGAAT
    TTTAGAGAGG TTAAAGCAGC TTTGGATATT GTGTCCGAGA GACTGTCTGC TTTCACAGAT
    GTGGGAAACA GCTGCTCACA TGTGGAGCAC ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA
    AAATCATGTG AAACTGTAGC AAAAGCCAGA ATGCTAGCTT CAGAGGGTGA TGCTTTTATT
    CAGAGCAATC ATTATGCTGT GGATTCCATT ATTCCAAAAT GCAGTGAGCT TCATCATCTA
    TGTGATGCCT TCACTACTGA GATGGAACAG AGGAGGAATG TTCTTAATAA ATCTCTAGAA
    CTGCATGGCT TACTGGAAAA GTCTATGAAG TGGTGCGATG AAGGAATTTA TCTTCTGGCT
    TCACAACCTG TAGATAAGTG TCAGTCTCAG GATGGTGCAG AATCAGCATT ACAGGAGATT
    GAGAAATTCC TAGACACTGG TGCAGGCAAT AAAATTAAGG AGTTGAATAA AGTTTACAGA
    GAGTATGCAC ACATCCTCAA TGAGGACCTA AAGGAGCATA TACAAAAGGT TTTCCAGAAG
    CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG AGACAAGTAA GTCTTAAGAA ACTGGCAGCT
    AAGCAAACAC GTCCTGTTCA GCCAGTTGCA CCTAGACCCG AAGCCTTTGT AAAATCTCCT
    TGTACCTCTC CAGGTCTTCA AAGAGATTGT GTATCCAACA CAGAAAGCGG TGTGCTTCGG
    AAAGTAAACT ACAGAAGAGG AAAGAGTGAA ATGAGTGAAC TCCATCAAAG CAGAGGAGGA
    TCTACAATGG ATGATGAAGA AAACCTAGCT GTGTTACGGC AGCATGTAAT GAATGAACTT
    ATTGAAACGG AACGAGCATA TGTGGAAGAA CTTCTCTGTG TTCTTGAGGG GTATGCTGCA
    GAAATGGACA ACCCCTTAAT GGCTCACCTC ATTTCACCAG AACTCCAAAA CAAGAAGGAT
    ATTTTGTTTG GGAATATGGA AGAAATTTAT CACTTTCACA ACAGGATATT CTTGAGAGAA
    CTAGAAAATT ATGTAGAATA CCCAGAACTG GTAGGACGGT GCTTTCTGGA TCAGATGGAA
    GATTTCCAGA TTTATGAGAA ATACTGTCAA AACAAACCTC GATCTGAAAG TTTGTGGAGG
    CAATTTTCAG ATTCCATATT CTTTCAGGAA TGTCAAAGGA AACTCGATCA CAAGCTCAGT
    TTGGACTCAT ACCTGCTGAA GCCAGTTCAA AGAATAACCA AATACCAATT ATTGCTAAAG
    GAGATGCTGA AGTACAGTAA GAACTGTGAA GGTGCTGAAG ATCTTCAGGA GGCATTAAAT
    TCTATATTGG GTATTCTCAA AGCAGTCAAT GATTCTATGC ACCAGATAGC CATCACAGGC
    TATGATGGAA ACCTGAATGA GCTGGGCAAG CTCCTAATGC AGGGATCCTT CAACGTCTGG
    ACTGATCATA AAAAGGGTCA CTCAAAGGTG AAAGATTTGG CACGCTTCAA GCCAATGCAG
    CGACACCTGT TCCTCCACGA GAAAGCAGTG CTGTTCTGTA AAAAGCGAGA AGAAAATGGA
    GAAGGATATG AGAAGGCACC TTCTTATAGC TATAAACACT CCTTAAATAT GGCAGCAGTT
    GGAATAACAG AAAATGTCAA AGGTGATGCA AAAAAATTTG AAATCTGGTA TAATGCAAGG
    GAAGAAGTTT ACATCATACA GGCACCAACT CCTGAAGTTA AAGCCACTTG GGTAAATGAA
    ATAAGAAAAG TGCTGACTAG TCAACTGCAG GCATGCAGAG AAGCCAGTCA ACATAGGACA
    CTTGAACACA CTCAGAGTTT ACCTCTACCA GCATCATCCT GTACAAGTCC TTCAAGGAAC
    AACATCAGAA ACGTGAAAAA AATGGATGAG AGAAAAGCTG ATCTCACAAG TCTAGAAGGT
    TATGTCACCA CAGTAGCACC AAAGTACCCC GAGAAAGGCA AAGATGACAC TAACTCTACC
    TCAGAATGCT CTGTACTGTC AAAAAAGCGC TTTACGCTGC AGGGCTTTAC TAACCTCAAA
    AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC AAAAAAGCTA AACGGCACGA AGTAGTGAGT
    GATCCAACTC CTTTTGGTGT AAGAGGTTGG GCTAAAACGT CTCATTCTCT TGATGCATCT
    GAAGAAAATG ATGGCTGGTC CAGTGCTGAG GAGCCGCTGA ATTCATCGGA TGCAGAAGAA
    GAAGGAGGAG GAGGAGGAGA GAGAAATGAG ATGAAGCTGA CTCCTGGTAA ATATACAGTT
    GTGTCTGACT ACGAGAAAGG AGTTTCTGAA GAATTTGCAG TGAAAGGTGG AGATCTAGTT
    CAACTGATTC GTGAAGGGGA GGAGGGACTT TGGTATGTGA AGAATCTGAC CACTGGTAGA
    GAAGGTTGGA TCCCAGCAAA CAATCTGCTG ATGCTCATAG GCAATTCAAA ATCAGCTCAA
    TCTTTGAGCA GCTCTGAATC AGGCACTGCT TCCAGCACTT TGAGCACGTC ATCAAGTTGC
    AGTGATAGCT GCAATGCCAG CAGCACCAGC TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133338.1
    CDS913..4383
    Translation

    Target ORF information:

    RefSeq Version XM_015277852.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277852.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCATCAGG ATATCATCCC CCTCTATGCA GCAGATATTC ATGACCAGTT AAAGAAACAA 
    TTTGCTTATC TGTCAGGTGG AAGAGGGGGA GATGGATGCC CTGTTATCAC TTTTCCCGAC
    TACCCAGCTT TCAGCGAGAT CCCTGAGAAG GAGTTCCAGA ATGTCCTGAC CTACCTGACC
    AGCATCCCGA GTTTACGAGA TGCTGGAATC GGGTTTATTC TTGTCATAGA CCGCAGACAG
    GACAAATGGA CTTCTGTGAA AGCTTCCATA CTGCGGATAG CAGCATCTTT CCCAGGGAAC
    TTGCAACTCG TCCTCGTTCT GCGCCCAACA GGACTTATTC ATCGAGCTCT CTCAGACATT
    GCTTTCAAGT TCAACAGAGA TGAGTTTAAA ATGAAAGTAC CTATCATAAT GCTGGGCTCA
    GTATCAGAAC TGCAGGGTTA CATTGATAAA ACACAATTAA CAGAGGATCT TGGTGGCACC
    CTGGATTACT GCCATAACAG ATGGCTATCC CTTCGCACTG CTATAGAAGG TTTTGCCCAA
    AAGGTTAAGC AAACAGCTCA GATTCTTCAG TCCTTTGGGA CTGAACTGGC TGAAACAGAA
    CTACCAAATG ATGTGCAGTC AACCAGCTCC CTTCTTGCAA TACATACGGA AAAGAAGGAC
    AAAATGAAGG AAGATATAAG GTTAGCAGTA GAACAGGGAG ACGATATCCT TGGAAGTATT
    ACAGAGCCAG TTACTGAGAA CCCTGAATAC AAGCTGAATC AAGACCAACT AGACAATCAA
    ACTACAGTAG AAAGGCTTTT GGCTCAGTTA CATGAAACAG AGTCCGCCTT TGATGAGTTT
    TGGATTAAGC ACCAGCAAAA ACTTGAGCAG TGTCTGCACC TTCGGAATTT TGAACAGAAT
    TTTAGAGAGG TTAAAGCAGC TTTGGATATT GTGTCCGAGA GACTGTCTGC TTTCACAGAT
    GTGGGAAACA GCTGCTCACA TGTGGAGCAC ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA
    AAATCATGTG AAACTGTAGC AAAAGCCAGA ATGCTAGCTT CAGAGGGTGA TGCTTTTATT
    CAGAGCAATC ATTATGCTGT GGATTCCATT ATTCCAAAAT GCAGTGAGCT TCATCATCTA
    TGTGATGCCT TCACTACTGA GATGGAACAG AGGAGGAATG TTCTTAATAA ATCTCTAGAA
    CTGCATGGCT TACTGGAAAA GTCTATGAAG TGGTGCGATG AAGGAATTTA TCTTCTGGCT
    TCACAACCTG TAGATAAGTG TCAGTCTCAG GATGGTGCAG AATCAGCATT ACAGGAGATT
    GAGAAATTCC TAGACACTGG TGCAGGCAAT AAAATTAAGG AGTTGAATAA AGTTTACAGA
    GAGTATGCAC ACATCCTCAA TGAGGACCTA AAGGAGCATA TACAAAAGGT TTTCCAGAAG
    CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG AGACAAGTAA GTCTTAAGAA ACTGGCAGCT
    AAGCAAACAC GTCCTGTTCA GCCAGTTGCA CCTAGACCCG AAGCCTTTGT AAAATCTCCT
    TGTACCTCTC CAGGTCTTCA AAGAGATTGT GTATCCAACA CAGAAAGCGG TGTGCTTCGG
    AAAGTAAACT ACAGAAGAGG AAAGAGTGAA ATGAGTGAAC TCCATCAAAG CAGAGGAGGA
    TCTACAATGG ATGATGAAGA AAACCTAGCT GTGTTACGGC AGCATGTAAT GAATGAACTT
    ATTGAAACGG AACGAGCATA TGTGGAAGAA CTTCTCTGTG TTCTTGAGGG GTATGCTGCA
    GAAATGGACA ACCCCTTAAT GGCTCACCTC ATTTCACCAG AACTCCAAAA CAAGAAGGAT
    ATTTTGTTTG GGAATATGGA AGAAATTTAT CACTTTCACA ACAGGATATT CTTGAGAGAA
    CTAGAAAATT ATGTAGAATA CCCAGAACTG GTAGGACGGT GCTTTCTGGA TCAGATGGAA
    GATTTCCAGA TTTATGAGAA ATACTGTCAA AACAAACCTC GATCTGAAAG TTTGTGGAGG
    CAATTTTCAG ATTCCATATT CTTTCAGGAA TGTCAAAGGA AACTCGATCA CAAGCTCAGT
    TTGGACTCAT ACCTGCTGAA GCCAGTTCAA AGAATAACCA AATACCAATT ATTGCTAAAG
    GAGATGCTGA AGTACAGTAA GAACTGTGAA GGTGCTGAAG ATCTTCAGGA GGCATTAAAT
    TCTATATTGG GTATTCTCAA AGCAGTCAAT GATTCTATGC ACCAGATAGC CATCACAGGC
    TATGATGGAA ACCTGAATGA GCTGGGCAAG CTCCTAATGC AGGGATCCTT CAACGTCTGG
    ACTGATCATA AAAAGGGTCA CTCAAAGGTG AAAGATTTGG CACGCTTCAA GCCAATGCAG
    CGACACCTGT TCCTCCACGA GAAAGCAGTG CTGTTCTGTA AAAAGCGAGA AGAAAATGGA
    GAAGGATATG AGAAGGCACC TTCTTATAGC TATAAACACT CCTTAAATAT GGCAGCAGTT
    GGAATAACAG AAAATGTCAA AGGTGATGCA AAAAAATTTG AAATCTGGTA TAATGCAAGG
    GAAGAAGTTT ACATCATACA GGCACCAACT CCTGAAGTTA AAGCCACTTG GGTAAATGAA
    ATAAGAAAAG TGCTGACTAG TCAACTGCAG GCATGCAGAG AAGCCAGTCA ACATAGGACA
    CTTGAACACA CTCAGAGTTT ACCTCTACCA GCATCATCCT GTACAAGTCC TTCAAGGAAC
    AACATCAGAA ACGTGAAAAA AATGGATGAG AGAAAAGCTG ATCTCACAAG TCTAGAAGGT
    TATGTCACCA CAGTAGCACC AAAGTACCCC GAGAAAGGCA AAGATGACAC TAACTCTACC
    TCAGAATGCT CTGTACTGTC AAAAAAGCGC TTTACGCTGC AGGGCTTTAC TAACCTCAAA
    AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC AAAAAAGCTA AACGGCACGA AGTAGTGAGT
    GATCCAACTC CTTTTGGTGT AAGAGGTTGG GCTAAAACGT CTCATTCTCT TGATGCATCT
    GAAGAAAATG ATGGCTGGTC CAGTGCTGAG GAGCCGCTGA ATTCATCGGA TGCAGAAGAA
    GAAGGAGGAG GAGGAGGAGA GAGAAATGAG ATGAAGCTGA CTCCTGGTAA ATATACAGTT
    GTGTCTGACT ACGAGAAAGG AGTTTCTGAA GAATTTGCAG TGAAAGGTGG AGATCTAGTT
    CAACTGATTC GTGAAGGGGA GGAGGGACTT TGGTATGTGA AGAATCTGAC CACTGGTAGA
    GAAGGTTGGA TCCCAGCAAA CAATCTGCTG ATGCTCATAG GCAATTCAAA ATCAGCTCAA
    TCTTTGAGCA GCTCTGAATC AGGCACTGCT TCCAGCACTT TGAGCACGTC ATCAAGTTGC
    AGTGATAGCT GCAATGCCAG CAGCACCAGC TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30678
    Clone ID Related Accession (Same CDS sequence) XM_015277854.1 , XM_015277854.2
    Accession Version XM_015277854.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3081bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAAGTAC CTATCATAAT GCTGGGCTCA GTATCAGAAC TGCAGGGTTA CATTGATAAA 
    ACACAATTAA CAGAGGATCT TGGTGGCACC CTGGATTACT GCCATAACAG ATGGCTATCC
    CTTCGCACTG CTATAGAAGG TTTTGCCCAA AAGGTTAAGC AAACAGCTCA GATTCTTCAG
    TCCTTTGGGA CTGAACTGGC TGAAACAGAA CTACCAAATG ATGTGCAGTC AACCAGCTCC
    CTTCTTGCAA TACATACGGA AAAGAAGGAC AAAATGAAGG AAGATATAAG GTTAGCAGTA
    GAACAGGGAG ACGATATCCT TGGAAGTATT ACAGAGCCAG TTACTGAGAA CCCTGAATAC
    AAGCTGAATC AAGACCAACT AGACAATCAA ACTACAGTAG AAAGGCTTTT GGCTCAGTTA
    CATGAAACAG AGTCCGCCTT TGATGAGTTT TGGATTAAGC ACCAGCAAAA ACTTGAGCAG
    TGTCTGCACC TTCGGAATTT TGAACAGAAT TTTAGAGAGG TTAAAGCAGC TTTGGATATT
    GTGTCCGAGA GACTGTCTGC TTTCACAGAT GTGGGAAACA GCTGCTCACA TGTGGAGCAC
    ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA AAATCATGTG AAACTGTAGC AAAAGCCAGA
    ATGCTAGCTT CAGAGGGTGA TGCTTTTATT CAGAGCAATC ATTATGCTGT GGATTCCATT
    ATTCCAAAAT GCAGTGAGCT TCATCATCTA TGTGATGCCT TCACTACTGA GATGGAACAG
    AGGAGGAATG TTCTTAATAA ATCTCTAGAA CTGCATGGCT TACTGGAAAA GTCTATGAAG
    TGGTGCGATG AAGGAATTTA TCTTCTGGCT TCACAACCTG TAGATAAGTG TCAGTCTCAG
    GATGGTGCAG AATCAGCATT ACAGGAGATT GAGAAATTCC TAGACACTGG TGCAGGCAAT
    AAAATTAAGG AGTTGAATAA AGTTTACAGA GAGTATGCAC ACATCCTCAA TGAGGACCTA
    AAGGAGCATA TACAAAAGGT TTTCCAGAAG CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG
    AGACAAGTAA GTCTTAAGAA ACTGGCAGCT AAGCAAACAC GTCCTGTTCA GCCAGTTGCA
    CCTAGACCCG AAGCCTTTGT AAAATCTCCT TGTACCTCTC CAGGTCTTCA AAGAGATTGT
    GTATCCAACA CAGAAAGCGG TGTGCTTCGG AAAGTAAACT ACAGAAGAGG AAAGAGTGAA
    ATGAGTGAAC TCCATCAAAG CAGAGGAGGA TCTACAATGG ATGATGAAGA AAACCTAGCT
    GTGTTACGGC AGCATGTAAT GAATGAACTT ATTGAAACGG AACGAGCATA TGTGGAAGAA
    CTTCTCTGTG TTCTTGAGGG GTATGCTGCA GAAATGGACA ACCCCTTAAT GGCTCACCTC
    ATTTCACCAG AACTCCAAAA CAAGAAGGAT ATTTTGTTTG GGAATATGGA AGAAATTTAT
    CACTTTCACA ACAGGATATT CTTGAGAGAA CTAGAAAATT ATGTAGAATA CCCAGAACTG
    GTAGGACGGT GCTTTCTGGA TCAGATGGAA GATTTCCAGA TTTATGAGAA ATACTGTCAA
    AACAAACCTC GATCTGAAAG TTTGTGGAGG CAATTTTCAG ATTCCATATT CTTTCAGGAA
    TGTCAAAGGA AACTCGATCA CAAGCTCAGT TTGGACTCAT ACCTGCTGAA GCCAGTTCAA
    AGAATAACCA AATACCAATT ATTGCTAAAG GAGATGCTGA AGTACAGTAA GAACTGTGAA
    GGTGCTGAAG ATCTTCAGGA GGCATTAAAT TCTATATTGG GTATTCTCAA AGCAGTCAAT
    GATTCTATGC ACCAGATAGC CATCACAGGC TATGATGGAA ACCTGAATGA GCTGGGCAAG
    CTCCTAATGC AGGGATCCTT CAACGTCTGG ACTGATCATA AAAAGGGTCA CTCAAAGGTG
    AAAGATTTGG CACGCTTCAA GCCAATGCAG CGACACCTGT TCCTCCACGA GAAAGCAGTG
    CTGTTCTGTA AAAAGCGAGA AGAAAATGGA GAAGGATATG AGAAGGCACC TTCTTATAGC
    TATAAACACT CCTTAAATAT GGCAGCAGTT GGAATAACAG AAAATGTCAA AGGTGATGCA
    AAAAAATTTG AAATCTGGTA TAATGCAAGG GAAGAAGTTT ACATCATACA GGCACCAACT
    CCTGAAGTTA AAGCCACTTG GGTAAATGAA ATAAGAAAAG TGCTGACTAG TCAACTGCAG
    GCATGCAGAG AAGCCAGTCA ACATAGGACA CTTGAACACA CTCAGAGTTT ACCTCTACCA
    GCATCATCCT GTACAAGTCC TTCAAGGAAC AACATCAGAA ACGTGAAAAA AATGGATGAG
    AGAAAAGCTG ATCTCACAAG TCTAGAAGGT TATGTCACCA CAGTAGCACC AAAGTACCCC
    GAGAAAGGCA AAGATGACAC TAACTCTACC TCAGAATGCT CTGTACTGTC AAAAAAGCGC
    TTTACGCTGC AGGGCTTTAC TAACCTCAAA AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC
    AAAAAAGCTA AACGGCACGA AGTAGTGAGT GATCCAACTC CTTTTGGTGT AAGAGGTTGG
    GCTAAAACGT CTCATTCTCT TGATGCATCT GAAGAAAATG ATGGCTGGTC CAGTGCTGAG
    GAGCCGCTGA ATTCATCGGA TGCAGAAGAA GAAGGAGGAG GAGGAGGAGA GAGAAATGAG
    ATGAAGCTGA CTCCTGGTAA ATATACAGTT GTGTCTGACT ACGAGAAAGG AGTTTCTGAA
    GAATTTGCAG TGAAAGGTGG AGATCTAGTT CAACTGATTC GTGAAGGGGA GGAGGGACTT
    TGGTATGTGA AGAATCTGAC CACTGGTAGA GAAGGTTGGA TCCCAGCAAA CAATCTGCTG
    ATGCTCATAG GCAATTCAAA ATCAGCTCAA TCTTTGAGCA GCTCTGAATC AGGCACTGCT
    TCCAGCACTT TGAGCACGTC ATCAAGTTGC AGTGATAGCT GCAATGCCAG CAGCACCAGC
    TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133340.1
    CDS758..3838
    Translation

    Target ORF information:

    RefSeq Version XM_015277854.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277854.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAAGTAC CTATCATAAT GCTGGGCTCA GTATCAGAAC TGCAGGGTTA CATTGATAAA 
    ACACAATTAA CAGAGGATCT TGGTGGCACC CTGGATTACT GCCATAACAG ATGGCTATCC
    CTTCGCACTG CTATAGAAGG TTTTGCCCAA AAGGTTAAGC AAACAGCTCA GATTCTTCAG
    TCCTTTGGGA CTGAACTGGC TGAAACAGAA CTACCAAATG ATGTGCAGTC AACCAGCTCC
    CTTCTTGCAA TACATACGGA AAAGAAGGAC AAAATGAAGG AAGATATAAG GTTAGCAGTA
    GAACAGGGAG ACGATATCCT TGGAAGTATT ACAGAGCCAG TTACTGAGAA CCCTGAATAC
    AAGCTGAATC AAGACCAACT AGACAATCAA ACTACAGTAG AAAGGCTTTT GGCTCAGTTA
    CATGAAACAG AGTCCGCCTT TGATGAGTTT TGGATTAAGC ACCAGCAAAA ACTTGAGCAG
    TGTCTGCACC TTCGGAATTT TGAACAGAAT TTTAGAGAGG TTAAAGCAGC TTTGGATATT
    GTGTCCGAGA GACTGTCTGC TTTCACAGAT GTGGGAAACA GCTGCTCACA TGTGGAGCAC
    ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA AAATCATGTG AAACTGTAGC AAAAGCCAGA
    ATGCTAGCTT CAGAGGGTGA TGCTTTTATT CAGAGCAATC ATTATGCTGT GGATTCCATT
    ATTCCAAAAT GCAGTGAGCT TCATCATCTA TGTGATGCCT TCACTACTGA GATGGAACAG
    AGGAGGAATG TTCTTAATAA ATCTCTAGAA CTGCATGGCT TACTGGAAAA GTCTATGAAG
    TGGTGCGATG AAGGAATTTA TCTTCTGGCT TCACAACCTG TAGATAAGTG TCAGTCTCAG
    GATGGTGCAG AATCAGCATT ACAGGAGATT GAGAAATTCC TAGACACTGG TGCAGGCAAT
    AAAATTAAGG AGTTGAATAA AGTTTACAGA GAGTATGCAC ACATCCTCAA TGAGGACCTA
    AAGGAGCATA TACAAAAGGT TTTCCAGAAG CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG
    AGACAAGTAA GTCTTAAGAA ACTGGCAGCT AAGCAAACAC GTCCTGTTCA GCCAGTTGCA
    CCTAGACCCG AAGCCTTTGT AAAATCTCCT TGTACCTCTC CAGGTCTTCA AAGAGATTGT
    GTATCCAACA CAGAAAGCGG TGTGCTTCGG AAAGTAAACT ACAGAAGAGG AAAGAGTGAA
    ATGAGTGAAC TCCATCAAAG CAGAGGAGGA TCTACAATGG ATGATGAAGA AAACCTAGCT
    GTGTTACGGC AGCATGTAAT GAATGAACTT ATTGAAACGG AACGAGCATA TGTGGAAGAA
    CTTCTCTGTG TTCTTGAGGG GTATGCTGCA GAAATGGACA ACCCCTTAAT GGCTCACCTC
    ATTTCACCAG AACTCCAAAA CAAGAAGGAT ATTTTGTTTG GGAATATGGA AGAAATTTAT
    CACTTTCACA ACAGGATATT CTTGAGAGAA CTAGAAAATT ATGTAGAATA CCCAGAACTG
    GTAGGACGGT GCTTTCTGGA TCAGATGGAA GATTTCCAGA TTTATGAGAA ATACTGTCAA
    AACAAACCTC GATCTGAAAG TTTGTGGAGG CAATTTTCAG ATTCCATATT CTTTCAGGAA
    TGTCAAAGGA AACTCGATCA CAAGCTCAGT TTGGACTCAT ACCTGCTGAA GCCAGTTCAA
    AGAATAACCA AATACCAATT ATTGCTAAAG GAGATGCTGA AGTACAGTAA GAACTGTGAA
    GGTGCTGAAG ATCTTCAGGA GGCATTAAAT TCTATATTGG GTATTCTCAA AGCAGTCAAT
    GATTCTATGC ACCAGATAGC CATCACAGGC TATGATGGAA ACCTGAATGA GCTGGGCAAG
    CTCCTAATGC AGGGATCCTT CAACGTCTGG ACTGATCATA AAAAGGGTCA CTCAAAGGTG
    AAAGATTTGG CACGCTTCAA GCCAATGCAG CGACACCTGT TCCTCCACGA GAAAGCAGTG
    CTGTTCTGTA AAAAGCGAGA AGAAAATGGA GAAGGATATG AGAAGGCACC TTCTTATAGC
    TATAAACACT CCTTAAATAT GGCAGCAGTT GGAATAACAG AAAATGTCAA AGGTGATGCA
    AAAAAATTTG AAATCTGGTA TAATGCAAGG GAAGAAGTTT ACATCATACA GGCACCAACT
    CCTGAAGTTA AAGCCACTTG GGTAAATGAA ATAAGAAAAG TGCTGACTAG TCAACTGCAG
    GCATGCAGAG AAGCCAGTCA ACATAGGACA CTTGAACACA CTCAGAGTTT ACCTCTACCA
    GCATCATCCT GTACAAGTCC TTCAAGGAAC AACATCAGAA ACGTGAAAAA AATGGATGAG
    AGAAAAGCTG ATCTCACAAG TCTAGAAGGT TATGTCACCA CAGTAGCACC AAAGTACCCC
    GAGAAAGGCA AAGATGACAC TAACTCTACC TCAGAATGCT CTGTACTGTC AAAAAAGCGC
    TTTACGCTGC AGGGCTTTAC TAACCTCAAA AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC
    AAAAAAGCTA AACGGCACGA AGTAGTGAGT GATCCAACTC CTTTTGGTGT AAGAGGTTGG
    GCTAAAACGT CTCATTCTCT TGATGCATCT GAAGAAAATG ATGGCTGGTC CAGTGCTGAG
    GAGCCGCTGA ATTCATCGGA TGCAGAAGAA GAAGGAGGAG GAGGAGGAGA GAGAAATGAG
    ATGAAGCTGA CTCCTGGTAA ATATACAGTT GTGTCTGACT ACGAGAAAGG AGTTTCTGAA
    GAATTTGCAG TGAAAGGTGG AGATCTAGTT CAACTGATTC GTGAAGGGGA GGAGGGACTT
    TGGTATGTGA AGAATCTGAC CACTGGTAGA GAAGGTTGGA TCCCAGCAAA CAATCTGCTG
    ATGCTCATAG GCAATTCAAA ATCAGCTCAA TCTTTGAGCA GCTCTGAATC AGGCACTGCT
    TCCAGCACTT TGAGCACGTC ATCAAGTTGC AGTGATAGCT GCAATGCCAG CAGCACCAGC
    TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30678
    Clone ID Related Accession (Same CDS sequence) XM_015277854.1 , XM_015277854.2
    Accession Version XM_015277854.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3081bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAAGTAC CTATCATAAT GCTGGGCTCA GTATCAGAAC TGCAGGGTTA CATTGATAAA 
    ACACAATTAA CAGAGGATCT TGGTGGCACC CTGGATTACT GCCATAACAG ATGGCTATCC
    CTTCGCACTG CTATAGAAGG TTTTGCCCAA AAGGTTAAGC AAACAGCTCA GATTCTTCAG
    TCCTTTGGGA CTGAACTGGC TGAAACAGAA CTACCAAATG ATGTGCAGTC AACCAGCTCC
    CTTCTTGCAA TACATACGGA AAAGAAGGAC AAAATGAAGG AAGATATAAG GTTAGCAGTA
    GAACAGGGAG ACGATATCCT TGGAAGTATT ACAGAGCCAG TTACTGAGAA CCCTGAATAC
    AAGCTGAATC AAGACCAACT AGACAATCAA ACTACAGTAG AAAGGCTTTT GGCTCAGTTA
    CATGAAACAG AGTCCGCCTT TGATGAGTTT TGGATTAAGC ACCAGCAAAA ACTTGAGCAG
    TGTCTGCACC TTCGGAATTT TGAACAGAAT TTTAGAGAGG TTAAAGCAGC TTTGGATATT
    GTGTCCGAGA GACTGTCTGC TTTCACAGAT GTGGGAAACA GCTGCTCACA TGTGGAGCAC
    ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA AAATCATGTG AAACTGTAGC AAAAGCCAGA
    ATGCTAGCTT CAGAGGGTGA TGCTTTTATT CAGAGCAATC ATTATGCTGT GGATTCCATT
    ATTCCAAAAT GCAGTGAGCT TCATCATCTA TGTGATGCCT TCACTACTGA GATGGAACAG
    AGGAGGAATG TTCTTAATAA ATCTCTAGAA CTGCATGGCT TACTGGAAAA GTCTATGAAG
    TGGTGCGATG AAGGAATTTA TCTTCTGGCT TCACAACCTG TAGATAAGTG TCAGTCTCAG
    GATGGTGCAG AATCAGCATT ACAGGAGATT GAGAAATTCC TAGACACTGG TGCAGGCAAT
    AAAATTAAGG AGTTGAATAA AGTTTACAGA GAGTATGCAC ACATCCTCAA TGAGGACCTA
    AAGGAGCATA TACAAAAGGT TTTCCAGAAG CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG
    AGACAAGTAA GTCTTAAGAA ACTGGCAGCT AAGCAAACAC GTCCTGTTCA GCCAGTTGCA
    CCTAGACCCG AAGCCTTTGT AAAATCTCCT TGTACCTCTC CAGGTCTTCA AAGAGATTGT
    GTATCCAACA CAGAAAGCGG TGTGCTTCGG AAAGTAAACT ACAGAAGAGG AAAGAGTGAA
    ATGAGTGAAC TCCATCAAAG CAGAGGAGGA TCTACAATGG ATGATGAAGA AAACCTAGCT
    GTGTTACGGC AGCATGTAAT GAATGAACTT ATTGAAACGG AACGAGCATA TGTGGAAGAA
    CTTCTCTGTG TTCTTGAGGG GTATGCTGCA GAAATGGACA ACCCCTTAAT GGCTCACCTC
    ATTTCACCAG AACTCCAAAA CAAGAAGGAT ATTTTGTTTG GGAATATGGA AGAAATTTAT
    CACTTTCACA ACAGGATATT CTTGAGAGAA CTAGAAAATT ATGTAGAATA CCCAGAACTG
    GTAGGACGGT GCTTTCTGGA TCAGATGGAA GATTTCCAGA TTTATGAGAA ATACTGTCAA
    AACAAACCTC GATCTGAAAG TTTGTGGAGG CAATTTTCAG ATTCCATATT CTTTCAGGAA
    TGTCAAAGGA AACTCGATCA CAAGCTCAGT TTGGACTCAT ACCTGCTGAA GCCAGTTCAA
    AGAATAACCA AATACCAATT ATTGCTAAAG GAGATGCTGA AGTACAGTAA GAACTGTGAA
    GGTGCTGAAG ATCTTCAGGA GGCATTAAAT TCTATATTGG GTATTCTCAA AGCAGTCAAT
    GATTCTATGC ACCAGATAGC CATCACAGGC TATGATGGAA ACCTGAATGA GCTGGGCAAG
    CTCCTAATGC AGGGATCCTT CAACGTCTGG ACTGATCATA AAAAGGGTCA CTCAAAGGTG
    AAAGATTTGG CACGCTTCAA GCCAATGCAG CGACACCTGT TCCTCCACGA GAAAGCAGTG
    CTGTTCTGTA AAAAGCGAGA AGAAAATGGA GAAGGATATG AGAAGGCACC TTCTTATAGC
    TATAAACACT CCTTAAATAT GGCAGCAGTT GGAATAACAG AAAATGTCAA AGGTGATGCA
    AAAAAATTTG AAATCTGGTA TAATGCAAGG GAAGAAGTTT ACATCATACA GGCACCAACT
    CCTGAAGTTA AAGCCACTTG GGTAAATGAA ATAAGAAAAG TGCTGACTAG TCAACTGCAG
    GCATGCAGAG AAGCCAGTCA ACATAGGACA CTTGAACACA CTCAGAGTTT ACCTCTACCA
    GCATCATCCT GTACAAGTCC TTCAAGGAAC AACATCAGAA ACGTGAAAAA AATGGATGAG
    AGAAAAGCTG ATCTCACAAG TCTAGAAGGT TATGTCACCA CAGTAGCACC AAAGTACCCC
    GAGAAAGGCA AAGATGACAC TAACTCTACC TCAGAATGCT CTGTACTGTC AAAAAAGCGC
    TTTACGCTGC AGGGCTTTAC TAACCTCAAA AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC
    AAAAAAGCTA AACGGCACGA AGTAGTGAGT GATCCAACTC CTTTTGGTGT AAGAGGTTGG
    GCTAAAACGT CTCATTCTCT TGATGCATCT GAAGAAAATG ATGGCTGGTC CAGTGCTGAG
    GAGCCGCTGA ATTCATCGGA TGCAGAAGAA GAAGGAGGAG GAGGAGGAGA GAGAAATGAG
    ATGAAGCTGA CTCCTGGTAA ATATACAGTT GTGTCTGACT ACGAGAAAGG AGTTTCTGAA
    GAATTTGCAG TGAAAGGTGG AGATCTAGTT CAACTGATTC GTGAAGGGGA GGAGGGACTT
    TGGTATGTGA AGAATCTGAC CACTGGTAGA GAAGGTTGGA TCCCAGCAAA CAATCTGCTG
    ATGCTCATAG GCAATTCAAA ATCAGCTCAA TCTTTGAGCA GCTCTGAATC AGGCACTGCT
    TCCAGCACTT TGAGCACGTC ATCAAGTTGC AGTGATAGCT GCAATGCCAG CAGCACCAGC
    TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133340.1
    CDS749..3829
    Translation

    Target ORF information:

    RefSeq Version XM_015277854.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277854.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAAGTAC CTATCATAAT GCTGGGCTCA GTATCAGAAC TGCAGGGTTA CATTGATAAA 
    ACACAATTAA CAGAGGATCT TGGTGGCACC CTGGATTACT GCCATAACAG ATGGCTATCC
    CTTCGCACTG CTATAGAAGG TTTTGCCCAA AAGGTTAAGC AAACAGCTCA GATTCTTCAG
    TCCTTTGGGA CTGAACTGGC TGAAACAGAA CTACCAAATG ATGTGCAGTC AACCAGCTCC
    CTTCTTGCAA TACATACGGA AAAGAAGGAC AAAATGAAGG AAGATATAAG GTTAGCAGTA
    GAACAGGGAG ACGATATCCT TGGAAGTATT ACAGAGCCAG TTACTGAGAA CCCTGAATAC
    AAGCTGAATC AAGACCAACT AGACAATCAA ACTACAGTAG AAAGGCTTTT GGCTCAGTTA
    CATGAAACAG AGTCCGCCTT TGATGAGTTT TGGATTAAGC ACCAGCAAAA ACTTGAGCAG
    TGTCTGCACC TTCGGAATTT TGAACAGAAT TTTAGAGAGG TTAAAGCAGC TTTGGATATT
    GTGTCCGAGA GACTGTCTGC TTTCACAGAT GTGGGAAACA GCTGCTCACA TGTGGAGCAC
    ATTTTGAAAG ATCTTGCCAA CTTTGAAGAA AAATCATGTG AAACTGTAGC AAAAGCCAGA
    ATGCTAGCTT CAGAGGGTGA TGCTTTTATT CAGAGCAATC ATTATGCTGT GGATTCCATT
    ATTCCAAAAT GCAGTGAGCT TCATCATCTA TGTGATGCCT TCACTACTGA GATGGAACAG
    AGGAGGAATG TTCTTAATAA ATCTCTAGAA CTGCATGGCT TACTGGAAAA GTCTATGAAG
    TGGTGCGATG AAGGAATTTA TCTTCTGGCT TCACAACCTG TAGATAAGTG TCAGTCTCAG
    GATGGTGCAG AATCAGCATT ACAGGAGATT GAGAAATTCC TAGACACTGG TGCAGGCAAT
    AAAATTAAGG AGTTGAATAA AGTTTACAGA GAGTATGCAC ACATCCTCAA TGAGGACCTA
    AAGGAGCATA TACAAAAGGT TTTCCAGAAG CAAGAAAGCA TGGAAGAAAT GTTTCAAAAG
    AGACAAGTAA GTCTTAAGAA ACTGGCAGCT AAGCAAACAC GTCCTGTTCA GCCAGTTGCA
    CCTAGACCCG AAGCCTTTGT AAAATCTCCT TGTACCTCTC CAGGTCTTCA AAGAGATTGT
    GTATCCAACA CAGAAAGCGG TGTGCTTCGG AAAGTAAACT ACAGAAGAGG AAAGAGTGAA
    ATGAGTGAAC TCCATCAAAG CAGAGGAGGA TCTACAATGG ATGATGAAGA AAACCTAGCT
    GTGTTACGGC AGCATGTAAT GAATGAACTT ATTGAAACGG AACGAGCATA TGTGGAAGAA
    CTTCTCTGTG TTCTTGAGGG GTATGCTGCA GAAATGGACA ACCCCTTAAT GGCTCACCTC
    ATTTCACCAG AACTCCAAAA CAAGAAGGAT ATTTTGTTTG GGAATATGGA AGAAATTTAT
    CACTTTCACA ACAGGATATT CTTGAGAGAA CTAGAAAATT ATGTAGAATA CCCAGAACTG
    GTAGGACGGT GCTTTCTGGA TCAGATGGAA GATTTCCAGA TTTATGAGAA ATACTGTCAA
    AACAAACCTC GATCTGAAAG TTTGTGGAGG CAATTTTCAG ATTCCATATT CTTTCAGGAA
    TGTCAAAGGA AACTCGATCA CAAGCTCAGT TTGGACTCAT ACCTGCTGAA GCCAGTTCAA
    AGAATAACCA AATACCAATT ATTGCTAAAG GAGATGCTGA AGTACAGTAA GAACTGTGAA
    GGTGCTGAAG ATCTTCAGGA GGCATTAAAT TCTATATTGG GTATTCTCAA AGCAGTCAAT
    GATTCTATGC ACCAGATAGC CATCACAGGC TATGATGGAA ACCTGAATGA GCTGGGCAAG
    CTCCTAATGC AGGGATCCTT CAACGTCTGG ACTGATCATA AAAAGGGTCA CTCAAAGGTG
    AAAGATTTGG CACGCTTCAA GCCAATGCAG CGACACCTGT TCCTCCACGA GAAAGCAGTG
    CTGTTCTGTA AAAAGCGAGA AGAAAATGGA GAAGGATATG AGAAGGCACC TTCTTATAGC
    TATAAACACT CCTTAAATAT GGCAGCAGTT GGAATAACAG AAAATGTCAA AGGTGATGCA
    AAAAAATTTG AAATCTGGTA TAATGCAAGG GAAGAAGTTT ACATCATACA GGCACCAACT
    CCTGAAGTTA AAGCCACTTG GGTAAATGAA ATAAGAAAAG TGCTGACTAG TCAACTGCAG
    GCATGCAGAG AAGCCAGTCA ACATAGGACA CTTGAACACA CTCAGAGTTT ACCTCTACCA
    GCATCATCCT GTACAAGTCC TTCAAGGAAC AACATCAGAA ACGTGAAAAA AATGGATGAG
    AGAAAAGCTG ATCTCACAAG TCTAGAAGGT TATGTCACCA CAGTAGCACC AAAGTACCCC
    GAGAAAGGCA AAGATGACAC TAACTCTACC TCAGAATGCT CTGTACTGTC AAAAAAGCGC
    TTTACGCTGC AGGGCTTTAC TAACCTCAAA AGTCAGAAAG CTTCTCCTAC CAGTCCTGAC
    AAAAAAGCTA AACGGCACGA AGTAGTGAGT GATCCAACTC CTTTTGGTGT AAGAGGTTGG
    GCTAAAACGT CTCATTCTCT TGATGCATCT GAAGAAAATG ATGGCTGGTC CAGTGCTGAG
    GAGCCGCTGA ATTCATCGGA TGCAGAAGAA GAAGGAGGAG GAGGAGGAGA GAGAAATGAG
    ATGAAGCTGA CTCCTGGTAA ATATACAGTT GTGTCTGACT ACGAGAAAGG AGTTTCTGAA
    GAATTTGCAG TGAAAGGTGG AGATCTAGTT CAACTGATTC GTGAAGGGGA GGAGGGACTT
    TGGTATGTGA AGAATCTGAC CACTGGTAGA GAAGGTTGGA TCCCAGCAAA CAATCTGCTG
    ATGCTCATAG GCAATTCAAA ATCAGCTCAA TCTTTGAGCA GCTCTGAATC AGGCACTGCT
    TCCAGCACTT TGAGCACGTC ATCAAGTTGC AGTGATAGCT GCAATGCCAG CAGCACCAGC
    TTCTCAGACA TTAAGGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa25957
    Clone ID Related Accession (Same CDS sequence) NM_001252063.1
    Accession Version NM_001252063.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-25
    Organism Gallus gallus(chicken)
    Product guanine nucleotide exchange factor DBS
    Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AC169087.3. On Nov 4, 2011 this sequence version replaced XM_416947.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGTTGCGG AATATAGATG CAAGTCTGTG AGACAGCTGG ATGCCCTAAG TAACTGGTCT 
    ACTTTTGAAG CAAATGATGC TCTGTTTGTT TTTGATTTAC AGAAATCAAC ACAGAGCCTT
    TTCATGTCTG ACTATTGGTA CTGGAGGCTG GTCCCGTGCA GAGAAACCGG CAGCTGTAAC
    TGTTTTGCAA CAGCCAACCA ATCTAATGAG GAGAAAGAAG AAGACACGAA CCACCAGGTT
    GTGGTGTCAG ACATCATGCA GCTGGTGCGA GACAAGCAGG AGGTGCCGGA AAGAACAGCA
    GATGAAATCA TGCATCAGGA TATCATCCCC CTCTATGCAG CAGATATTCA TGACCAGTTA
    AAGAAACAAT TTGCTTATCT GTCAGGTGGA AGAGGGGGAG ATGGATGCCC TGTTATCACT
    TTTCCCGACT ACCCAGCTTT CAGCGAGATC CCTGAGAAGG AGTTCCAGAA TGTCCTGACC
    TACCTGACCA GCATCCCGAG TTTACGAGAT GCTGGAATCG GGTTTATTCT TGTCATAGAC
    CGCAGACAGG ACAAATGGAC TTCTGTGAAA GCTTCCATAC TGCGGATAGC AGCATCTTTC
    CCAGGGAACT TGCAACTCGT CCTCGTTCTG CGCCCAACAG GACTTATTCA TCGAGCTCTC
    TCAGACATTG CTTTCAAGTT CAACAGAGAT GAGTTTAAAA TGAAAGTACC TATCATAATG
    CTGGGCTCAG TATCAGAACT GCAGGGTTAC ATTGATAAAA CACAATTAAC AGAGGATCTT
    GGTGGCACCC TGGATTACTG CCATAACAGA TGGCTATCCC TTCGCACTGC TATAGAAGGT
    TTTGCCCAAA AGGTTAAGCA AACAGCTCAG ATTCTTCAGT CCTTTGGGAC TGAACTGGCT
    GAAACAGAAC TACCAAATGA TGTGCAGTCA ACCAGCTCCC TTCTTGCAAT ACATACGGAA
    AAGAAGGACA AAATGAAGGA AGATATAAGG TTAGCAGTAG AACAGGGAGA CGATATCCTT
    GGAAGTATTA CAGAGCCAGT TACTGAGAAC CCTGAATACA AGCTGAATCA AGACCAACTA
    GACAATCAAA CTACAGTAGA AAGGCTTTTG GCTCAGTTAC ATGAAACAGA GTCCGCCTTT
    GATGAGTTTT GGATTAAGCA CCAGCAAAAA CTTGAGCAGT GTCTGCACCT TCGGAATTTT
    GAACAGAATT TTAGAGAGGT TAAAGCAGCT TTGGATATTG TGTCCGAGAG ACTGTCTGCT
    TTCACAGATG TGGGAAACAG CTGCTCACAT GTGGAGCACA TTTTGAAAGA TCTTGCCAAC
    TTTGAAGAAA AATCATGTGA AACTGTAGCA AAAGCCAGAA TGCTAGCTTC AGAGGGTGAT
    GCTTTTATTC AGAGCAATCA TTATGCTGTG GATTCCATTA TTCCAAAATG CAGTGAGCTT
    CATCATCTAT GTGATGCCTT CACTACTGAG ATGGAACAGA GGAGGAATGT TCTTAATAAA
    TCTCTAGAAC TGCATGGCTT ACTGGAAAAG TCTATGAAGT GGTGCGATGA AGGAATTTAT
    CTTCTGGCTT CACAACCTGT AGATAAGTGT CAGTCTCAGG ATGGTGCAGA ATCAGCATTA
    CAGGAGATTG AGAAATTCCT AGACACTGGT GCAGGCAATA AAATTAAGGA GTTGAATAAA
    GTTTACAGAG AGTATGCACA CATCCTCAAT GAGGACCTAA AGGAGCATAT ACAAAAGGTT
    TTCCAGAAGC AAGAAAGCAT GGAAGAAATG TTTCAAAAGA GACAAGTAAG TCTTAAGAAA
    CTGGCAGCTA AGCAAACACG TCCTGTTCAG CCAGTTGCAC CTAGACCCGA AGCCTTTGTA
    AAATCTCCTT GTACCTCTCC AGGTCTTCAA AGAGATTGTG TATCCAACAC AGAAAGCGGT
    GTGCTTCGGA AAGTAAACTA CAGAAGAGGA AAGAGTGAAA TGAGTGAACT CCATCAAAGC
    AGAGGAGGAT CTACAATGGA TGATGAAGAA AACCTAGCTG TGTTACGGCA GCATGTAATG
    AATGAACTTA TTGAAACGGA ACGAGCATAT GTGGAAGAAC TTCTCTGTGT TCTTGAGGGG
    TATGCTGCAG AAATGGACAA CCCCTTAATG GCTCACCTCA TTTCACCAGA ACTCCAAAAC
    AAGAAGGATA TTTTGTTTGG GAATATGGAA GAAATTTATC ACTTTCACAA CAGGATATTC
    TTGAGAGAAC TAGAAAATTA TGTAGAATAC CCAGAACTGG TAGGACGGTG CTTTCTGGAT
    CAGATGGAAG ATTTCCAGAT TTATGAGAAA TACTGTCAAA ACAAACCTCG ATCTGAAAGT
    TTGTGGAGGC AATTTTCAGA TTCCATATTC TTTCAGGAAT GTCAAAGGAA ACTCGATCAC
    AAGCTCAGTT TGGACTCATA CCTGCTGAAG CCAGTTCAAA GAATAACCAA ATACCAATTA
    TTGCTAAAGG AGATGCTGAA GTACAGTAAG AACTGTGAAG GTGCTGAAGA TCTTCAGGAG
    GCATTAAATT CTATATTGGG TATTCTCAAA GCAGTCAATG ATTCTATGCA CCAGATAGCC
    ATCACAGGCT ATGATGGAAA CCTGAATGAG CTGGGCAAGC TCCTAATGCA GGGATCCTTC
    AACGTCTGGA CTGATCATAA AAAGGGTCAC TCAAAGGTGA AAGATTTGGC ACGCTTCAAG
    CCAATGCAGC GACACCTGTT CCTCCACGAG AAAGCAGTGC TGTTCTGTAA AAAGCGAGAA
    GAAAATGGAG AAGGATATGA GAAGGCACCT TCTTATAGCT ATAAACACTC CTTAAATATG
    GCAGCAGTTG GAATAACAGA AAATGTCAAA GGTGATGCAA AAAAATTTGA AATCTGGTAT
    AATGCAAGGG AAGAAGTTTA CATCATACAG GCACCAACTC CTGAAGTTAA AGCCACTTGG
    GTAAATGAAA TAAGAAAAGT GCTGACTAGT CAACTGCAGG CATGCAGAGA AGCCAGTCAA
    CATAGGACAC TTGAACACAC TCAGAGTTTA CCTCTACCAG CATCATCCTG TACAAGTCCT
    TCAAGGAACA ACATCAGAAA CGTGAAAAAA ATGGATGAGA GAAAAGCTGA TCTCACAAGT
    CTAGAAGGTT ATGTCACCAC AGTAGCACCA AAGTACCCCG AGAAAGGCAA AGATGACACT
    AACTCTACCT CAGAATGCTC TGTACTGTCA AAAAAGCGCT TTACGCTGCA GGGCTTTACT
    AACCTCAAAA GTCAGAAAGC TTCTCCTACC AGTCCTGACA AAAAAGCTAA ACGGCACGAA
    GTAGTGAGTG ATCCAACTCC TTTTGGTGTA AGAGGTTGGG CTAAAACGTC TCATTCTCTT
    GATGCATCTG AAGAAAATGA TGGCTGGTCC AGTGCTGAGG AGCCGCTGAA TTCATCGGAT
    GCAGAAGAAG AAGGAGGAGG AGGAGGAGAG AGAAATGAGA TGAAGCTGAC TCCTGGTAAA
    TATACAGTTG TGTCTGACTA CGAGAAAGGA GTTTCTGAAG AATTTGCAGT GAAAGGTGGA
    GATCTAGTTC AACTGATTCG TGAAGGGGAG GAGGGACTTT GGTATGTGAA GAATCTGACC
    ACTGGTAGAG AAGGTTGGAT CCCAGCAAAC AATCTGCTGA TGCTCATAGG CAATTCAAAA
    TCAGCTCAAT CTTTGAGCAG CTCTGAATCA GGCACTGCTT CCAGCACTTT GAGCACGTCA
    TCAAGTTGCA GTGATAGCTG CAATGCCAGC AGCACCAGCT TCTCAGACAT TAAGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001238992.1
    CDS1..3780
    Translation

    Target ORF information:

    RefSeq Version NM_001252063.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence like (MCF2L), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001252063.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGTTGCGG AATATAGATG CAAGTCTGTG AGACAGCTGG ATGCCCTAAG TAACTGGTCT 
    ACTTTTGAAG CAAATGATGC TCTGTTTGTT TTTGATTTAC AGAAATCAAC ACAGAGCCTT
    TTCATGTCTG ACTATTGGTA CTGGAGGCTG GTCCCGTGCA GAGAAACCGG CAGCTGTAAC
    TGTTTTGCAA CAGCCAACCA ATCTAATGAG GAGAAAGAAG AAGACACGAA CCACCAGGTT
    GTGGTGTCAG ACATCATGCA GCTGGTGCGA GACAAGCAGG AGGTGCCGGA AAGAACAGCA
    GATGAAATCA TGCATCAGGA TATCATCCCC CTCTATGCAG CAGATATTCA TGACCAGTTA
    AAGAAACAAT TTGCTTATCT GTCAGGTGGA AGAGGGGGAG ATGGATGCCC TGTTATCACT
    TTTCCCGACT ACCCAGCTTT CAGCGAGATC CCTGAGAAGG AGTTCCAGAA TGTCCTGACC
    TACCTGACCA GCATCCCGAG TTTACGAGAT GCTGGAATCG GGTTTATTCT TGTCATAGAC
    CGCAGACAGG ACAAATGGAC TTCTGTGAAA GCTTCCATAC TGCGGATAGC AGCATCTTTC
    CCAGGGAACT TGCAACTCGT CCTCGTTCTG CGCCCAACAG GACTTATTCA TCGAGCTCTC
    TCAGACATTG CTTTCAAGTT CAACAGAGAT GAGTTTAAAA TGAAAGTACC TATCATAATG
    CTGGGCTCAG TATCAGAACT GCAGGGTTAC ATTGATAAAA CACAATTAAC AGAGGATCTT
    GGTGGCACCC TGGATTACTG CCATAACAGA TGGCTATCCC TTCGCACTGC TATAGAAGGT
    TTTGCCCAAA AGGTTAAGCA AACAGCTCAG ATTCTTCAGT CCTTTGGGAC TGAACTGGCT
    GAAACAGAAC TACCAAATGA TGTGCAGTCA ACCAGCTCCC TTCTTGCAAT ACATACGGAA
    AAGAAGGACA AAATGAAGGA AGATATAAGG TTAGCAGTAG AACAGGGAGA CGATATCCTT
    GGAAGTATTA CAGAGCCAGT TACTGAGAAC CCTGAATACA AGCTGAATCA AGACCAACTA
    GACAATCAAA CTACAGTAGA AAGGCTTTTG GCTCAGTTAC ATGAAACAGA GTCCGCCTTT
    GATGAGTTTT GGATTAAGCA CCAGCAAAAA CTTGAGCAGT GTCTGCACCT TCGGAATTTT
    GAACAGAATT TTAGAGAGGT TAAAGCAGCT TTGGATATTG TGTCCGAGAG ACTGTCTGCT
    TTCACAGATG TGGGAAACAG CTGCTCACAT GTGGAGCACA TTTTGAAAGA TCTTGCCAAC
    TTTGAAGAAA AATCATGTGA AACTGTAGCA AAAGCCAGAA TGCTAGCTTC AGAGGGTGAT
    GCTTTTATTC AGAGCAATCA TTATGCTGTG GATTCCATTA TTCCAAAATG CAGTGAGCTT
    CATCATCTAT GTGATGCCTT CACTACTGAG ATGGAACAGA GGAGGAATGT TCTTAATAAA
    TCTCTAGAAC TGCATGGCTT ACTGGAAAAG TCTATGAAGT GGTGCGATGA AGGAATTTAT
    CTTCTGGCTT CACAACCTGT AGATAAGTGT CAGTCTCAGG ATGGTGCAGA ATCAGCATTA
    CAGGAGATTG AGAAATTCCT AGACACTGGT GCAGGCAATA AAATTAAGGA GTTGAATAAA
    GTTTACAGAG AGTATGCACA CATCCTCAAT GAGGACCTAA AGGAGCATAT ACAAAAGGTT
    TTCCAGAAGC AAGAAAGCAT GGAAGAAATG TTTCAAAAGA GACAAGTAAG TCTTAAGAAA
    CTGGCAGCTA AGCAAACACG TCCTGTTCAG CCAGTTGCAC CTAGACCCGA AGCCTTTGTA
    AAATCTCCTT GTACCTCTCC AGGTCTTCAA AGAGATTGTG TATCCAACAC AGAAAGCGGT
    GTGCTTCGGA AAGTAAACTA CAGAAGAGGA AAGAGTGAAA TGAGTGAACT CCATCAAAGC
    AGAGGAGGAT CTACAATGGA TGATGAAGAA AACCTAGCTG TGTTACGGCA GCATGTAATG
    AATGAACTTA TTGAAACGGA ACGAGCATAT GTGGAAGAAC TTCTCTGTGT TCTTGAGGGG
    TATGCTGCAG AAATGGACAA CCCCTTAATG GCTCACCTCA TTTCACCAGA ACTCCAAAAC
    AAGAAGGATA TTTTGTTTGG GAATATGGAA GAAATTTATC ACTTTCACAA CAGGATATTC
    TTGAGAGAAC TAGAAAATTA TGTAGAATAC CCAGAACTGG TAGGACGGTG CTTTCTGGAT
    CAGATGGAAG ATTTCCAGAT TTATGAGAAA TACTGTCAAA ACAAACCTCG ATCTGAAAGT
    TTGTGGAGGC AATTTTCAGA TTCCATATTC TTTCAGGAAT GTCAAAGGAA ACTCGATCAC
    AAGCTCAGTT TGGACTCATA CCTGCTGAAG CCAGTTCAAA GAATAACCAA ATACCAATTA
    TTGCTAAAGG AGATGCTGAA GTACAGTAAG AACTGTGAAG GTGCTGAAGA TCTTCAGGAG
    GCATTAAATT CTATATTGGG TATTCTCAAA GCAGTCAATG ATTCTATGCA CCAGATAGCC
    ATCACAGGCT ATGATGGAAA CCTGAATGAG CTGGGCAAGC TCCTAATGCA GGGATCCTTC
    AACGTCTGGA CTGATCATAA AAAGGGTCAC TCAAAGGTGA AAGATTTGGC ACGCTTCAAG
    CCAATGCAGC GACACCTGTT CCTCCACGAG AAAGCAGTGC TGTTCTGTAA AAAGCGAGAA
    GAAAATGGAG AAGGATATGA GAAGGCACCT TCTTATAGCT ATAAACACTC CTTAAATATG
    GCAGCAGTTG GAATAACAGA AAATGTCAAA GGTGATGCAA AAAAATTTGA AATCTGGTAT
    AATGCAAGGG AAGAAGTTTA CATCATACAG GCACCAACTC CTGAAGTTAA AGCCACTTGG
    GTAAATGAAA TAAGAAAAGT GCTGACTAGT CAACTGCAGG CATGCAGAGA AGCCAGTCAA
    CATAGGACAC TTGAACACAC TCAGAGTTTA CCTCTACCAG CATCATCCTG TACAAGTCCT
    TCAAGGAACA ACATCAGAAA CGTGAAAAAA ATGGATGAGA GAAAAGCTGA TCTCACAAGT
    CTAGAAGGTT ATGTCACCAC AGTAGCACCA AAGTACCCCG AGAAAGGCAA AGATGACACT
    AACTCTACCT CAGAATGCTC TGTACTGTCA AAAAAGCGCT TTACGCTGCA GGGCTTTACT
    AACCTCAAAA GTCAGAAAGC TTCTCCTACC AGTCCTGACA AAAAAGCTAA ACGGCACGAA
    GTAGTGAGTG ATCCAACTCC TTTTGGTGTA AGAGGTTGGG CTAAAACGTC TCATTCTCTT
    GATGCATCTG AAGAAAATGA TGGCTGGTCC AGTGCTGAGG AGCCGCTGAA TTCATCGGAT
    GCAGAAGAAG AAGGAGGAGG AGGAGGAGAG AGAAATGAGA TGAAGCTGAC TCCTGGTAAA
    TATACAGTTG TGTCTGACTA CGAGAAAGGA GTTTCTGAAG AATTTGCAGT GAAAGGTGGA
    GATCTAGTTC AACTGATTCG TGAAGGGGAG GAGGGACTTT GGTATGTGAA GAATCTGACC
    ACTGGTAGAG AAGGTTGGAT CCCAGCAAAC AATCTGCTGA TGCTCATAGG CAATTCAAAA
    TCAGCTCAAT CTTTGAGCAG CTCTGAATCA GGCACTGCTT CCAGCACTTT GAGCACGTCA
    TCAAGTTGCA GTGATAGCTG CAATGCCAGC AGCACCAGCT TCTCAGACAT TAAGGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.