RB1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	RB1
Entrez Gene ID	386582
Full Name	retinoblastoma 1
General protein information	Preferred Names retinoblastoma 1 Names retinoblastoma-associated protein p104 pRb rb retinoblastoma 1 (including osteosarcoma)
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204419.1	NP_989750.1	retinoblastoma-associated protein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RB1	NP_001070375.1
Homo sapiens (human)	RB1	NP_000312.2
Mus musculus (house mouse)	Rb1	NP_033055.2
Gallus gallus (chicken)	RB1	NP_989750.1
Pan troglodytes (chimpanzee)	RB1	XP_509777.3
Rattus norvegicus (Norway rat)	Rb1	NP_058741.1
Danio rerio (zebrafish)	rb1	NP_001071248.1
Xenopus tropicalis (tropical clawed frog)	rb1	XP_002935161.2
Canis lupus familiaris (dog)	RB1	XP_534118.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene pathways and cell cycle-related genes in cultured avian primordial germ cells.
Rengaraj D, Lee BR, Choi JW, Lee SI, Seo HW, Kim TH, Choi HJ, Song G, Han JY
Poultry science91(12)3167-77(2012 Dec)

Fine-mapping of quantitative trait loci for body weight and bone traits and positional cloning of the RB1 gene in chicken.
Zhang H, Liu SH, Zhang Q, Zhang YD, Wang SZ, Wang QG, Wang YX, Tang ZQ, Li H
Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie128(5)366-75(2011 Oct)

Early developmental expression pattern of retinoblastoma tumor suppressor mRNA indicates a role in the epithelial-to-mesenchyme transformation of endocardial cushion cells.
Wagner M, Miles K, Siddiqui MA
Developmental dynamics : an official publication of the American Association of Anatomists220(3)198-211(2001 Mar)

Characterization of a chicken cDNA encoding the retinoblastoma gene product.
Feinstein R, Bolton WK, Quinones JN, Mosialos G, Sif S, Huff JL, Capobianco AJ, Gilmore TD
Biochimica et biophysica acta1218(1)82-6(1994 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Experiments showed an essential role of pRb in S-phase entry of the supporting cells in the damaged neonatal chicken utricle and have shown that the proliferation of these cells requires the phosphorylation of pRb
Title: pRb phosphorylation regulates the proliferation of supporting cells in gentamicin-damaged neonatal avian utricle.

the RB1 gene was a major gene for body weight and bone traits
Title: Fine-mapping of quantitative trait loci for body weight and bone traits and positional cloning of the RB1 gene in chicken.

The following RB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa27226	NM_204419.1 Latest version!	Gallus gallus retinoblastoma 1 (RB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RB1 ( NM_204419.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989750.1 Gallus gallus retinoblastoma 1 (RB1), mRNA.

Price & Availability↑

CloneID	OGa27226
Clone ID Related Accession (Same CDS sequence)	NM_204419.1
Accession Version	NM_204419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Gallus gallus(chicken)
Product	retinoblastoma-associated protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X72218.1. On Jan 18, 2005 this sequence version replaced XM_429234.1. Sequence Note: This sequence has been modified as follows: removed 77 bp suspected to be vector contamination from the 3' end. ##Evidence-Data-START## CDS exon combination :: X72218.1, U00113.1 [ECO:0000331] ##Evidence-Data-END##

ATGCCGCCCA AGCCCCTTCG CAGGGCGGGC GCCGCCAGGA GTCAGCGCAC CAGCCCCGAG 
GGCGGCGCCG GCACCGCCTC ACCGCCCGGC GGCACCCGGC TGGAAGTGGG GGAAGCGGAG 
TTCGTGGCTC TGTGCGATGC GCTGAAGGCG CCGGACAGCG TGAGGGAGAA GGCCTGGATG 
ACGTACCAGA GCTTGGCGGC CGCCGACGGG GCTTCGGCTT ATAACAAAAA GAAGAAGGAA 
ACATGGGGAG TCTGTATCTT TATTGTAGCA ATTGATCTGG ATGAAATGAC ATTCACTTTT 
ACAGAGCTGC TGAAAAGTTT AAGCATAAGC GTGTGCACAT TTTTTCAGTT TCTCAAAGAG 
GTGGATGTTA ACATGGATAC TGTAAGCACT AAAGTTGATA GCACTGTATC AAGGCTGAAA 
AAGAAATACG ATGTCTTACT TGCACTGTAT CACAAGTTCG AAAGGACTTG TGGCCTTATT 
TATCTGGAGC AACCCAGTAG TGAGATTTCT GCTGAACTCA GTTCTGTATT AGTCCTAAAA 
AATTACTGGA TTACTTTTTT GCTGGCAAAA GGTAAAGTGT TGCAAATGGA AGATGACCTC 
GTGATCTCTT TCCAGTTGTT GCTGTGCGTC TTAGATTATT TTATCAAACT GTCACCTCCT 
GCTATGCTCA AGGAACCATA CAAGTCTGCT GTGACTGCAC TGACTGTTAA TGGTTCAACT 
CGAACCCCAC GAAGAGGTCA GAACAGGAAT GCACGCGCTT CAAAGCAAAT TGATACAGAT 
ACAAAAGTTA TTGAAATCCT TTGTAAAGAG CATGATTGTA ATTTAGACGA GGTAAAAAAT 
GTGTATTTCA CAAGCTTTAT TCCTTTCTTG AATTCTCTTG GTGTTGTAGC CTCAAATGGA 
TTACCAGAGG TTGACGTTCT CTCAAAACAA TATGATGAGC TTTATCTCAA AAACAAAGAT 
ATAGATGCAA GATTATTTCT GGATCATGAT GAAACTCTAC AGCCTGATGT TATAGCATGC 
TCACAGTTGG AAAGAACACC ACGAAAAAAT AACCCAGATG AGGAGGTTAA TCATGTACTT 
CCACAGACTC CTGTTCGAGC TGCAATGAAT ACTATTCAGC AGTTGATGAT GATTTTGAAC 
TCAGCAACTG ATAAACCATC AGATACTCTC ATTGCATATT TCAATAATTG CACAGTGAAC 
CCTGAAGACA GCATCCTGAA AAGAGTAGAA AGTCTTGGCC ACATCTTCAA AAAGAAATTT 
GCTGAAGCAG TTGGACAGGG ATGTGCTGAG ATTGGCTCAC AGAGATACCA GCTTGGAGTA 
CGGCTGTATT ACAGAGTAAT GGAGTCTATG CTGAAGTCGG AAGAAGAGCG CCTCTCAGTG 
CACAATTTTA GCAAACTTCT GAATGATAAC ATCTTCCACA CATCTCTTCT GGCATGTGCT 
CTTGAAATTG TCATGGCTAC CTATGGCAGA ACTGCTTCAC AAAGTGATGG TACCAGTGCT 
GAAACAGACT TGTCTTTCCC ATGGATTCTC AACGTATTTG ATTTAAAAGC TTTTGACTTC 
TACAAGGTGA TTGAAAGTTT CATTAAAGTG GAGCCAAGCC TAACGAGGGA CATGATAAAG 
CATCTAGAAC GCTGTGAACA TCGCATTATG GAATCTCTTG CTTGGCAATC TGATTCACCT 
CTGTTCGATC TTATCAAGCA GTCAAAAGAA CGAGAAGGTC AGACCGATCA GCCTGAGCCC 
ACTTCCACCC TAAATCTGCC TCTCCAGCAC AACCACACTG CAGCAGATCT GTATCTTTCT 
CCTGTGAGAT CTCCTAAGAA GAAAGCATCT GGACACCCTC AAAGTGGTAC TTCTAATCCA 
GATGCTCAGC CATCTGCGAC CTCACAAACA CAGAAGCCAC AGAAATCTAC CTCCCTCTCT 
CTGTTCTACA AAAAAGTGTT TCGACTGGCC TATCTTCGAT TGCATACCCT GTTCTTTCGG 
CTTCTCTCCG AACATCCTGA CCTGGAGCCG TTGATCTGGA CCCTCTTCCA GCACACACTG 
CAAAATGAGT CTGAGCTTAT GAGAGACAGG CACTTGGACC AGATCATGAT GTGTTCCATG 
TATGGCATAT GCAAAGTAAA GAATGTGGAT CTTAGATTTA AAACAATAGT TTCTGCATAC 
AAAGAGCTGC CCAATACAAA TCAAGAGACT TTCAAACGTG TTCTTATCAG GGAAGAGCAG 
TACGACTCCA TTATAGTCTT CTACAACTTA GTGTTCATGC AGAAGCTGAA GACAAACATC 
CTGCAGTATG CCTCCAACAG GCCTCCTACG CTGTCACCTA TCCCACACAT TCCTCGCAGC 
CCCTACCAGT TTTCCAATTC TCCTCGGCGT GTCCCTGCGG GCAATAACAT CTACATCTCA 
CCCTTGAAGA GCCCTTACAA GTTCTCTGAT GGGTTTCATT CTCCCACAAA GATGACTCCG 
AGGTCCAGAA TTCTGGTGTC AATTGGTGAA ACGTTTGGGA CTTCTGAAAA GTTTCAAAAA 
ATAAACCAGA TGGTGTGCAA CAGCGAGAGC CACGTAAAGC GCAGTGCGGA GCCCAGCGAT 
GCCCCCAAGC CACTGAAGAG GCTGCGCTTC GATATAGAAG GGCAGGACGA GGCAGATGGA 
GGCAAACACC TTCCCCAGGA GTCCAAGTTC CAACAAAAGC TTGCAGAAAT GACATCTACT 
CGAACAAGAA TGCAGAAGCA GAAACTGAAT GATGGAAACG ATACCTCAGC CAACGAAGAA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989750.1
CDS	50..2815
Translation	MPPKPLRRAGAARSQRTSPEGGAGTASPPGGTRLEVGEAEFVALCDALKAPDSVREKAWMTYQSLAAADGASAYNKKKKETWGVCIFIVAIDLDEMTFTFTELLKSLSISVCTFFQFLKEVDVNMDTVSTKVDSTVSRLKKKYDVLLALYHKFERTCGLIYLEQPSSEISAELSSVLVLKNYWITFLLAKGKVLQMEDDLVISFQLLLCVLDYFIKLSPPAMLKEPYKSAVTALTVNGSTRTPRRGQNRNARASKQIDTDTKVIEILCKEHDCNLDEVKNVYFTSFIPFLNSLGVVASNGLPEVDVLSKQYDELYLKNKDIDARLFLDHDETLQPDVIACSQLERTPRKNNPDEEVNHVLPQTPVRAAMNTIQQLMMILNSATDKPSDTLIAYFNNCTVNPEDSILKRVESLGHIFKKKFAEAVGQGCAEIGSQRYQLGVRLYYRVMESMLKSEEERLSVHNFSKLLNDNIFHTSLLACALEIVMATYGRTASQSDGTSAETDLSFPWILNVFDLKAFDFYKVIESFIKVEPSLTRDMIKHLERCEHRIMESLAWQSDSPLFDLIKQSKEREGQTDQPEPTSTLNLPLQHNHTAADLYLSPVRSPKKKASGHPQSGTSNPDAQPSATSQTQKPQKSTSLSLFYKKVFRLAYLRLHTLFFRLLSEHPDLEPLIWTLFQHTLQNESELMRDRHLDQIMMCSMYGICKVKNVDLRFKTIVSAYKELPNTNQETFKRVLIREEQYDSIIVFYNLVFMQKLKTNILQYASNRPPTLSPIPHIPRSPYQFSNSPRRVPAGNNIYISPLKSPYKFSDGFHSPTKMTPRSRILVSIGETFGTSEKFQKINQMVCNSESHVKRSAEPSDAPKPLKRLRFDIEGQDEADGGKHLPQESKFQQKLAEMTSTRTRMQKQKLNDGNDTSANEEK

Target ORF information:

RefSeq Version	NM_204419.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus retinoblastoma 1 (RB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204419.1

ORF Insert Sequence:

ATGCCGCCCA AGCCCCTTCG CAGGGCGGGC GCCGCCAGGA GTCAGCGCAC CAGCCCCGAG 
GGCGGCGCCG GCACCGCCTC ACCGCCCGGC GGCACCCGGC TGGAAGTGGG GGAAGCGGAG 
TTCGTGGCTC TGTGCGATGC GCTGAAGGCG CCGGACAGCG TGAGGGAGAA GGCCTGGATG 
ACGTACCAGA GCTTGGCGGC CGCCGACGGG GCTTCGGCTT ATAACAAAAA GAAGAAGGAA 
ACATGGGGAG TCTGTATCTT TATTGTAGCA ATTGATCTGG ATGAAATGAC ATTCACTTTT 
ACAGAGCTGC TGAAAAGTTT AAGCATAAGC GTGTGCACAT TTTTTCAGTT TCTCAAAGAG 
GTGGATGTTA ACATGGATAC TGTAAGCACT AAAGTTGATA GCACTGTATC AAGGCTGAAA 
AAGAAATACG ATGTCTTACT TGCACTGTAT CACAAGTTCG AAAGGACTTG TGGCCTTATT 
TATCTGGAGC AACCCAGTAG TGAGATTTCT GCTGAACTCA GTTCTGTATT AGTCCTAAAA 
AATTACTGGA TTACTTTTTT GCTGGCAAAA GGTAAAGTGT TGCAAATGGA AGATGACCTC 
GTGATCTCTT TCCAGTTGTT GCTGTGCGTC TTAGATTATT TTATCAAACT GTCACCTCCT 
GCTATGCTCA AGGAACCATA CAAGTCTGCT GTGACTGCAC TGACTGTTAA TGGTTCAACT 
CGAACCCCAC GAAGAGGTCA GAACAGGAAT GCACGCGCTT CAAAGCAAAT TGATACAGAT 
ACAAAAGTTA TTGAAATCCT TTGTAAAGAG CATGATTGTA ATTTAGACGA GGTAAAAAAT 
GTGTATTTCA CAAGCTTTAT TCCTTTCTTG AATTCTCTTG GTGTTGTAGC CTCAAATGGA 
TTACCAGAGG TTGACGTTCT CTCAAAACAA TATGATGAGC TTTATCTCAA AAACAAAGAT 
ATAGATGCAA GATTATTTCT GGATCATGAT GAAACTCTAC AGCCTGATGT TATAGCATGC 
TCACAGTTGG AAAGAACACC ACGAAAAAAT AACCCAGATG AGGAGGTTAA TCATGTACTT 
CCACAGACTC CTGTTCGAGC TGCAATGAAT ACTATTCAGC AGTTGATGAT GATTTTGAAC 
TCAGCAACTG ATAAACCATC AGATACTCTC ATTGCATATT TCAATAATTG CACAGTGAAC 
CCTGAAGACA GCATCCTGAA AAGAGTAGAA AGTCTTGGCC ACATCTTCAA AAAGAAATTT 
GCTGAAGCAG TTGGACAGGG ATGTGCTGAG ATTGGCTCAC AGAGATACCA GCTTGGAGTA 
CGGCTGTATT ACAGAGTAAT GGAGTCTATG CTGAAGTCGG AAGAAGAGCG CCTCTCAGTG 
CACAATTTTA GCAAACTTCT GAATGATAAC ATCTTCCACA CATCTCTTCT GGCATGTGCT 
CTTGAAATTG TCATGGCTAC CTATGGCAGA ACTGCTTCAC AAAGTGATGG TACCAGTGCT 
GAAACAGACT TGTCTTTCCC ATGGATTCTC AACGTATTTG ATTTAAAAGC TTTTGACTTC 
TACAAGGTGA TTGAAAGTTT CATTAAAGTG GAGCCAAGCC TAACGAGGGA CATGATAAAG 
CATCTAGAAC GCTGTGAACA TCGCATTATG GAATCTCTTG CTTGGCAATC TGATTCACCT 
CTGTTCGATC TTATCAAGCA GTCAAAAGAA CGAGAAGGTC AGACCGATCA GCCTGAGCCC 
ACTTCCACCC TAAATCTGCC TCTCCAGCAC AACCACACTG CAGCAGATCT GTATCTTTCT 
CCTGTGAGAT CTCCTAAGAA GAAAGCATCT GGACACCCTC AAAGTGGTAC TTCTAATCCA 
GATGCTCAGC CATCTGCGAC CTCACAAACA CAGAAGCCAC AGAAATCTAC CTCCCTCTCT 
CTGTTCTACA AAAAAGTGTT TCGACTGGCC TATCTTCGAT TGCATACCCT GTTCTTTCGG 
CTTCTCTCCG AACATCCTGA CCTGGAGCCG TTGATCTGGA CCCTCTTCCA GCACACACTG 
CAAAATGAGT CTGAGCTTAT GAGAGACAGG CACTTGGACC AGATCATGAT GTGTTCCATG 
TATGGCATAT GCAAAGTAAA GAATGTGGAT CTTAGATTTA AAACAATAGT TTCTGCATAC 
AAAGAGCTGC CCAATACAAA TCAAGAGACT TTCAAACGTG TTCTTATCAG GGAAGAGCAG 
TACGACTCCA TTATAGTCTT CTACAACTTA GTGTTCATGC AGAAGCTGAA GACAAACATC 
CTGCAGTATG CCTCCAACAG GCCTCCTACG CTGTCACCTA TCCCACACAT TCCTCGCAGC 
CCCTACCAGT TTTCCAATTC TCCTCGGCGT GTCCCTGCGG GCAATAACAT CTACATCTCA 
CCCTTGAAGA GCCCTTACAA GTTCTCTGAT GGGTTTCATT CTCCCACAAA GATGACTCCG 
AGGTCCAGAA TTCTGGTGTC AATTGGTGAA ACGTTTGGGA CTTCTGAAAA GTTTCAAAAA 
ATAAACCAGA TGGTGTGCAA CAGCGAGAGC CACGTAAAGC GCAGTGCGGA GCCCAGCGAT 
GCCCCCAAGC CACTGAAGAG GCTGCGCTTC GATATAGAAG GGCAGGACGA GGCAGATGGA 
GGCAAACACC TTCCCCAGGA GTCCAAGTTC CAACAAAAGC TTGCAGAAAT GACATCTACT 
CGAACAAGAA TGCAGAAGCA GAAACTGAAT GATGGAAACG ATACCTCAGC CAACGAAGAA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene pathways and cell cycle-related genes in cultured avian primordial germ cells.
Poultry science91(12)3167-77(2012 Dec)
Rengaraj D,Lee BR,Choi JW,Lee SI,Seo HW,Kim TH,Choi HJ,Song G,Han JY

Fine-mapping of quantitative trait loci for body weight and bone traits and positional cloning of the RB1 gene in chicken.
Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie128(5)366-75(2011 Oct)
Zhang H,Liu SH,Zhang Q,Zhang YD,Wang SZ,Wang QG,Wang YX,Tang ZQ,Li H

Early developmental expression pattern of retinoblastoma tumor suppressor mRNA indicates a role in the epithelial-to-mesenchyme transformation of endocardial cushion cells.
Developmental dynamics : an official publication of the American Association of Anatomists220(3)198-211(2001 Mar)
Wagner M,Miles K,Siddiqui MA

Characterization of a chicken cDNA encoding the retinoblastoma gene product.
Biochimica et biophysica acta1218(1)82-6(1994 May)
Feinstein R,Bolton WK,Quinones JN,Mosialos G,Sif S,Huff JL,Capobianco AJ,Gilmore TD

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RB1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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