Rb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rb1
Entrez Gene ID	24708
Full Name	RB transcriptional corepressor 1
General protein information	Preferred Names RB transcriptional corepressor 1 Names retinoblastoma-associated protein Retinoblastoma 1 (including osteosarcoma) pRb pp105
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15q11
Summary	plays a role in negative regulation of the cell cycle and of DNA replication; acts as a tumor suppressor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017045.1	NP_058741.1	retinoblastoma-associated protein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RB1	NP_001070375.1
Homo sapiens (human)	RB1	NP_000312.2
Mus musculus (house mouse)	Rb1	NP_033055.2
Gallus gallus (chicken)	RB1	NP_989750.1
Pan troglodytes (chimpanzee)	RB1	XP_509777.3
Rattus norvegicus (Norway rat)	Rb1	NP_058741.1
Danio rerio (zebrafish)	rb1	NP_001071248.1
Xenopus tropicalis (tropical clawed frog)	rb1	XP_002935161.2
Canis lupus familiaris (dog)	RB1	XP_534118.4

Gene Ontology
Bibliography

Related articles in PubMed

Site-specific hyperphosphorylation of pRb in HIV-induced neurotoxicity.
Akay C, Lindl KA, Wang Y, White MG, Isaacman-Beck J, Kolson DL, Jordan-Sciutto KL
Molecular and cellular neurosciences47(2)154-65(2011 Jun)

Early cyst growth is associated with the increased nuclear expression of cyclin D1/Rb protein in an autosomal-recessive polycystic kidney disease rat model.
Schwensen KG, Burgess JS, Graf NS, Alexander SI, Harris DC, Phillips JK, Rangan GK
Nephron. Experimental nephrology117(4)e93-103(2011)

JC virus small T antigen binds phosphatase PP2A and Rb family proteins and is required for efficient viral DNA replication activity.
Bollag B, Hofstetter CA, Reviriego-Mendoza MM, Frisque RJ
PloS one5(5)e10606(2010 May)

ATM is involved in cell-cycle control through the regulation of retinoblastoma protein phosphorylation.
Pizarro JG, Folch J, de la Torre AV, Junyent F, Verdaguer E, Jordan J, Pallas M, Camins A
Journal of cellular biochemistry110(1)210-8(2010 May)

Imbalance of tumor suppression genes expression following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide.
Ribeiro DA, Fracalossi AC, Uatari SA, Oshima CT, Salvadori DM
In vivo (Athens, Greece)23(6)937-42(2009 Nov-Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Identify Dyrk1a as a novel negative regulator of D-cyclin-mediated Rb1/E2f-signalling. As dysregulation of this pathway with impaired cardiomyocyte proliferation leads to cardiomyopathy.
Title: Dyrk1a regulates the cardiomyocyte cell cycle via D-cyclin-dependent Rb/E2f-signalling.

Inflammation disrupts LDLr feedback regulation through the activation of the mTOR pathway, increasing mTORC1 activity and up-regulating SREBP-2-mediated cholesterol uptake through Rb phosphorylation.
Title: Activation of mTOR modulates SREBP-2 to induce foam cell formation through increased retinoblastoma protein phosphorylation.

Phosphorylated retinoblastoma protein (p-Rb) is involved in neuronal apoptosis after traumatic brain injury in adult rats.
Title: Phosphorylated retinoblastoma protein (p-Rb) is involved in neuronal apoptosis after traumatic brain injury in adult rats.

a role for Rb protein in directing oncogenic cell growth
Title: Loss of Rb cooperates with Ras to drive oncogenic growth in mammalian cells.

Results indicate that HIV-infection in the brain is associated with site-specific hyperphosphorylation of pRb at serine795, and that this phosphorylation contributes to neuronal death in this disease.
Title: Site-specific hyperphosphorylation of pRb in HIV-induced neurotoxicity.

The following Rb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa39889	NM_017045.1 Latest version!	Rattus norvegicus retinoblastoma 1 (Rb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rb1 ( NM_017045.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058741.1 Rattus norvegicus retinoblastoma 1 (Rb1), mRNA.

Price & Availability↑

CloneID	ORa39889
Clone ID Related Accession (Same CDS sequence)	NM_017045.1
Accession Version	NM_017045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2012-04-28
Organism	Rattus norvegicus(Norway rat)
Product	retinoblastoma-associated protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR03095290.1, D25233.1 and BE098611.1. On or before Dec 18, 2009 this sequence version replaced XM_344434.3, XM_001071121.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGCCGCCCA AAGCCCCGCG CAGAACCGCG GCCGCCGAGC CCCCGCCGCC GCCGCCGCCG 
CCTCCGGAGG ACGACCCCGC GCAGGACAGT GACCCCGAAG AGCTGCCCCT GATCAGGCTT 
GAGTTTGAAA AAATTGAAGA ACCTGAATTT ATTGCATTAT GTCAGAAGTT AAAGGTGCCC 
GATCATGTCA GAGAGAGAGC TTGGCTAACG TGGGAGAAAG TTTCATCTGT GGATGGAATC 
CTGGAAGGAT ATATTCAGAA GAAGAAGGAA CTCTGGGGCA TCTGCATCTT CATTGCAGCA 
GTTGACCTGG ATGAGATGCC ATTCACTTTT ACTGAGCTTC AGAAAAGCAT AGAAACCAGT 
GTCTATAAAT TCTTTGACTT ATTAAAAGAA ATTGATACCA GTACCAAAGT TGATAATGCT 
GTGTCAAGGC TATTGAAGAA GTACAATGTG TTATGTGCAC TCTACAGCAA ATTAGAACGG 
ACATGTGGAC TTATATATTT GACACAGCCC AGCAGTGGGT TATCTACTGA AATAAATTCT 
ATGTTGGTGC TAAAAGTTTC TTGGATCACC TTTTTACTAG CTAAAGGAGA AGTAGTACAG 
ATGGAAGATG ATCTGGTGAT CTCATTTCAG TTAATGTTGT GTGTACTTGA CTATTTTATC 
AAGCTCTCGC CTCCTGCGCT GCTCAGAGAG CCGTACAAAA CAGCTGCAAC ACCCATTAAT 
GGTTCACCTC GGACACCCAG AAGAGGTCAG AACAGGAGTG CTCGGATAGC AAAACAACTA 
GAAAGTGATA CAAGAACTAT TGAAGTTCTC TGTAAGGAGC ACGAGTGTAA TGTAGATGAG 
GTGAAAAATG TTTATTTCAA AAATTTTATC CCTTTTATAA GTTCACTTGG AATTGTATCA 
TCTAATGGAC TTCCAGAGCT TGAAAGTCTT TCTAAACGCT ACGAAGAAGT TTATCTTAAA 
AGCAAAGATT TAGATGCAAG ACTATTTTTG GATCATGACA AAACACTTCA GACTGATACT 
ATAGACAGTT TTGAAACAGA GAGAACTCCA CGAAAAAGCA ACCCTGATGA AGAGGCAAAC 
ATGGTTACTC CACACACTCC AGTTAGGACT GTTATGAATA CTATTCAACA GTTAATGGTG 
ATTTTAAATT CTGCAAGTGA TCAGCCATCA GAAAATCTGA TTTCCTACTT CAGTAATTGC 
ACAGTGAATC CAAAAGAAAA TATCCTAAAA AGAGTAAAGG ATGTTGGACA CATCTTTAAA 
GAGAAGTTTG CTAGCGCTGT GGGGCAGGGA TGTATTGACA TTGGAGCACA GCGATATAAA 
CTTGGGGTTC GATTGTATTA TCGTGTGATG GAATCGATGC TTAAATCAGA AGAAGAACGT 
TTGTCCATTC AGAATTTTAG CAAACTCCTA AATGACAACA TCTTTCATAT GTCTTTATTG 
GCCTGTGCTC TTGAAGTTGT AATGGCTACA TATAGCAGGA GTATGTTACA GAATCTTGAT 
TCTGGAACAG ATTTGTCCTT CCCGTGGATT CTGAATGTAC TTAATTTAAA AGCCTTTGAT 
TTTTACAAAG TGATTGAAAG TTTTATCAAA GTGGAAGCCA ACTTGACAAG AGAAATGATA 
AAACATTTAG AAAGATGTGA GCATCGAATC ATGGAATCCC TTGCATGGCT TTCTGATTCA 
CCTTTGTTTG ATCTCATAAA GCAGTCCAAG GATGGGGAAG GACCTGATCA CCTTGAGTCT 
GCTTGTTCTC TCAGCCTCCC TCTCCAGAGT AACCACACTG CAGCAGATAT GTATCTTTCT 
CCTATAAGAT CTCCAAAGAA AAGAACTTCT ACTACACGTG TAAATTCTGC GGCAAATACA 
GAGACGCAAG CAGCCTCAGC CTTCCATACT CAGAAGCCAT TGAAATCTAC CTCCCTTTCC 
CTGTTTTACA AAAAAGTGTA CCGTCTAGCA TATCTCCGAC TAAATACACT GTGTGCGCGC 
CTTCTGTCTG ACCACCCAGA GCCAGAACAC ATCATCTGGA CTCTGTTTCA GCATACATTG 
GAAAATGAGT ATGAGCTCAT GAAAGACCGA CATTTGGACC AGATTATGAT GTGTTCTATG 
TATGGCATCT GCAAGGTGAA AAACATTGAC CTTAAATTCA AAATCATCGT AACTGCGTAT 
AAGGATCTTC CTCACGCTGC CCAGGAGACC TTTAAACGTG TTTTGATCAG AGAAGAGGAG 
TTTGATTCCA TTATAGTGTT CTACAACTCA GTTTTCATGC AGAGACTAAA AACAAATATT 
TTACAGTATG CCTCCACCAG GCCTCCTACC TTGTCACCAA TACCTCACAT TCCTCGAAGC 
CCTTACAAGT TCTCGAGTTC ACCCTTGCGG ATTCCTGGAG GTAACATCTA TATGTCACCC 
CTAAAGAGTC CTTATAAAAT TTCAGAAGGT CTGCCAACAC CCACAAAAAT GACTCCAAGA 
TCAAGAATCT TGGTCTCAAT CGGTGAATCA TTTGGGACAT CCGAGAAGTT CCAGAAAATA 
AACCAGATGG TGTGTAACAG TGACAGAGTG CTCAAAAGAA GTGCTGAAGG CGGCAATCCC 
CCCAAACCCC TGAAGAAGCT GCGCTTTGAC ATCGAAGGAT CCGATGAAGC AGACGGGAGT 
AAACATCTCC CAGCGGAGTC CAAATTCCAA CAGAAACTGG CAGAAATGAC TTCTACTCGA 
ACACGAATGC AAAAGCAGAA ACTGAATGAT AGCATGGAAA TCTCAAACAA GGAGGAAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058741.1
CDS	177..2939
Translation	MPPKAPRRTAAAEPPPPPPPPPEDDPAQDSDPEELPLIRLEFEKIEEPEFIALCQKLKVPDHVRERAWLTWEKVSSVDGILEGYIQKKKELWGICIFIAAVDLDEMPFTFTELQKSIETSVYKFFDLLKEIDTSTKVDNAVSRLLKKYNVLCALYSKLERTCGLIYLTQPSSGLSTEINSMLVLKVSWITFLLAKGEVVQMEDDLVISFQLMLCVLDYFIKLSPPALLREPYKTAATPINGSPRTPRRGQNRSARIAKQLESDTRTIEVLCKEHECNVDEVKNVYFKNFIPFISSLGIVSSNGLPELESLSKRYEEVYLKSKDLDARLFLDHDKTLQTDTIDSFETERTPRKSNPDEEANMVTPHTPVRTVMNTIQQLMVILNSASDQPSENLISYFSNCTVNPKENILKRVKDVGHIFKEKFASAVGQGCIDIGAQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSMLQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKVEANLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDGEGPDHLESACSLSLPLQSNHTAADMYLSPIRSPKKRTSTTRVNSAANTETQAASAFHTQKPLKSTSLSLFYKKVYRLAYLRLNTLCARLLSDHPEPEHIIWTLFQHTLENEYELMKDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAAQETFKRVLIREEEFDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFSSSPLRIPGGNIYMSPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGGNPPKPLKKLRFDIEGSDEADGSKHLPAESKFQQKLAEMTSTRTRMQKQKLNDSMEISNKEEK

Target ORF information:

RefSeq Version	NM_017045.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus retinoblastoma 1 (Rb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017045.1

ORF Insert Sequence:

ATGCCGCCCA AAGCCCCGCG CAGAACCGCG GCCGCCGAGC CCCCGCCGCC GCCGCCGCCG 
CCTCCGGAGG ACGACCCCGC GCAGGACAGT GACCCCGAAG AGCTGCCCCT GATCAGGCTT 
GAGTTTGAAA AAATTGAAGA ACCTGAATTT ATTGCATTAT GTCAGAAGTT AAAGGTGCCC 
GATCATGTCA GAGAGAGAGC TTGGCTAACG TGGGAGAAAG TTTCATCTGT GGATGGAATC 
CTGGAAGGAT ATATTCAGAA GAAGAAGGAA CTCTGGGGCA TCTGCATCTT CATTGCAGCA 
GTTGACCTGG ATGAGATGCC ATTCACTTTT ACTGAGCTTC AGAAAAGCAT AGAAACCAGT 
GTCTATAAAT TCTTTGACTT ATTAAAAGAA ATTGATACCA GTACCAAAGT TGATAATGCT 
GTGTCAAGGC TATTGAAGAA GTACAATGTG TTATGTGCAC TCTACAGCAA ATTAGAACGG 
ACATGTGGAC TTATATATTT GACACAGCCC AGCAGTGGGT TATCTACTGA AATAAATTCT 
ATGTTGGTGC TAAAAGTTTC TTGGATCACC TTTTTACTAG CTAAAGGAGA AGTAGTACAG 
ATGGAAGATG ATCTGGTGAT CTCATTTCAG TTAATGTTGT GTGTACTTGA CTATTTTATC 
AAGCTCTCGC CTCCTGCGCT GCTCAGAGAG CCGTACAAAA CAGCTGCAAC ACCCATTAAT 
GGTTCACCTC GGACACCCAG AAGAGGTCAG AACAGGAGTG CTCGGATAGC AAAACAACTA 
GAAAGTGATA CAAGAACTAT TGAAGTTCTC TGTAAGGAGC ACGAGTGTAA TGTAGATGAG 
GTGAAAAATG TTTATTTCAA AAATTTTATC CCTTTTATAA GTTCACTTGG AATTGTATCA 
TCTAATGGAC TTCCAGAGCT TGAAAGTCTT TCTAAACGCT ACGAAGAAGT TTATCTTAAA 
AGCAAAGATT TAGATGCAAG ACTATTTTTG GATCATGACA AAACACTTCA GACTGATACT 
ATAGACAGTT TTGAAACAGA GAGAACTCCA CGAAAAAGCA ACCCTGATGA AGAGGCAAAC 
ATGGTTACTC CACACACTCC AGTTAGGACT GTTATGAATA CTATTCAACA GTTAATGGTG 
ATTTTAAATT CTGCAAGTGA TCAGCCATCA GAAAATCTGA TTTCCTACTT CAGTAATTGC 
ACAGTGAATC CAAAAGAAAA TATCCTAAAA AGAGTAAAGG ATGTTGGACA CATCTTTAAA 
GAGAAGTTTG CTAGCGCTGT GGGGCAGGGA TGTATTGACA TTGGAGCACA GCGATATAAA 
CTTGGGGTTC GATTGTATTA TCGTGTGATG GAATCGATGC TTAAATCAGA AGAAGAACGT 
TTGTCCATTC AGAATTTTAG CAAACTCCTA AATGACAACA TCTTTCATAT GTCTTTATTG 
GCCTGTGCTC TTGAAGTTGT AATGGCTACA TATAGCAGGA GTATGTTACA GAATCTTGAT 
TCTGGAACAG ATTTGTCCTT CCCGTGGATT CTGAATGTAC TTAATTTAAA AGCCTTTGAT 
TTTTACAAAG TGATTGAAAG TTTTATCAAA GTGGAAGCCA ACTTGACAAG AGAAATGATA 
AAACATTTAG AAAGATGTGA GCATCGAATC ATGGAATCCC TTGCATGGCT TTCTGATTCA 
CCTTTGTTTG ATCTCATAAA GCAGTCCAAG GATGGGGAAG GACCTGATCA CCTTGAGTCT 
GCTTGTTCTC TCAGCCTCCC TCTCCAGAGT AACCACACTG CAGCAGATAT GTATCTTTCT 
CCTATAAGAT CTCCAAAGAA AAGAACTTCT ACTACACGTG TAAATTCTGC GGCAAATACA 
GAGACGCAAG CAGCCTCAGC CTTCCATACT CAGAAGCCAT TGAAATCTAC CTCCCTTTCC 
CTGTTTTACA AAAAAGTGTA CCGTCTAGCA TATCTCCGAC TAAATACACT GTGTGCGCGC 
CTTCTGTCTG ACCACCCAGA GCCAGAACAC ATCATCTGGA CTCTGTTTCA GCATACATTG 
GAAAATGAGT ATGAGCTCAT GAAAGACCGA CATTTGGACC AGATTATGAT GTGTTCTATG 
TATGGCATCT GCAAGGTGAA AAACATTGAC CTTAAATTCA AAATCATCGT AACTGCGTAT 
AAGGATCTTC CTCACGCTGC CCAGGAGACC TTTAAACGTG TTTTGATCAG AGAAGAGGAG 
TTTGATTCCA TTATAGTGTT CTACAACTCA GTTTTCATGC AGAGACTAAA AACAAATATT 
TTACAGTATG CCTCCACCAG GCCTCCTACC TTGTCACCAA TACCTCACAT TCCTCGAAGC 
CCTTACAAGT TCTCGAGTTC ACCCTTGCGG ATTCCTGGAG GTAACATCTA TATGTCACCC 
CTAAAGAGTC CTTATAAAAT TTCAGAAGGT CTGCCAACAC CCACAAAAAT GACTCCAAGA 
TCAAGAATCT TGGTCTCAAT CGGTGAATCA TTTGGGACAT CCGAGAAGTT CCAGAAAATA 
AACCAGATGG TGTGTAACAG TGACAGAGTG CTCAAAAGAA GTGCTGAAGG CGGCAATCCC 
CCCAAACCCC TGAAGAAGCT GCGCTTTGAC ATCGAAGGAT CCGATGAAGC AGACGGGAGT 
AAACATCTCC CAGCGGAGTC CAAATTCCAA CAGAAACTGG CAGAAATGAC TTCTACTCGA 
ACACGAATGC AAAAGCAGAA ACTGAATGAT AGCATGGAAA TCTCAAACAA GGAGGAAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Site-specific hyperphosphorylation of pRb in HIV-induced neurotoxicity.
Molecular and cellular neurosciences47(2)154-65(2011 Jun)
Akay C,Lindl KA,Wang Y,White MG,Isaacman-Beck J,Kolson DL,Jordan-Sciutto KL

Early cyst growth is associated with the increased nuclear expression of cyclin D1/Rb protein in an autosomal-recessive polycystic kidney disease rat model.
Nephron. Experimental nephrology117(4)e93-103(2011)
Schwensen KG,Burgess JS,Graf NS,Alexander SI,Harris DC,Phillips JK,Rangan GK

JC virus small T antigen binds phosphatase PP2A and Rb family proteins and is required for efficient viral DNA replication activity.
PloS one5(5)e10606(2010 May)
Bollag B,Hofstetter CA,Reviriego-Mendoza MM,Frisque RJ

ATM is involved in cell-cycle control through the regulation of retinoblastoma protein phosphorylation.
Journal of cellular biochemistry110(1)210-8(2010 May)
Pizarro JG,Folch J,de la Torre AV,Junyent F,Verdaguer E,Jordan J,Pallas M,Camins A

Imbalance of tumor suppression genes expression following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide.
In vivo (Athens, Greece)23(6)937-42(2009 Nov-Dec)
Ribeiro DA,Fracalossi AC,Uatari SA,Oshima CT,Salvadori DM

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Rb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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