RB1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RB1
Entrez Gene ID	5925
Full Name	RB transcriptional corepressor 1
Synonyms	OSRC,PPP1R130,RB,p105-Rb,pRb,pp110
General protein information	Preferred Names RB transcriptional corepressor 1 Names retinoblastoma-associated protein GOS563 exon 17 substitution mutation causes premature stop exon 17 tumor GOS561 substitution mutation causes premature stop prepro-retinoblastoma-associated protein protein phosphatase 1, regulatory subunit 130 retinoblastoma 1 retinoblastoma suspectibility protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q14.2
Summary	The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008].
Disorder MIM:	614041
Disorder Html:	Retinoblastoma, 180200 (3); Osteosarcoma, somatic, 259500 (3); Bladder cancer, somatic, 109800 (3); Small cell cancer of the lung, somatic, 182280 (3); Retinoblastoma, trilateral, 180200 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011535171.2	XP_011533473.1	retinoblastoma-associated protein isoform X1
NM_000321.2	NP_000312.2	retinoblastoma-associated protein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RB1	NP_001070375.1
Homo sapiens (human)	RB1	NP_000312.2
Mus musculus (house mouse)	Rb1	NP_033055.2
Gallus gallus (chicken)	RB1	NP_989750.1
Pan troglodytes (chimpanzee)	RB1	XP_509777.3
Rattus norvegicus (Norway rat)	Rb1	NP_058741.1
Danio rerio (zebrafish)	rb1	NP_001071248.1
Xenopus tropicalis (tropical clawed frog)	rb1	XP_002935161.2
Canis lupus familiaris (dog)	RB1	XP_534118.4

Gene Ontology

The following RB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu25417	NM_000321.2 Latest version!	Homo sapiens RB transcriptional corepressor 1 (RB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu60211	XM_011535171.2 Latest version!	Homo sapiens RB transcriptional corepressor 1 (RB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RB1 ( NM_000321.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000312.2 Homo sapiens RB transcriptional corepressor 1 (RB1), mRNA.

Price & Availability↑

CloneID	OHu25417
Clone ID Related Accession (Same CDS sequence)	NM_000321.2
Accession Version	NM_000321.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Homo sapiens(human)
Product	retinoblastoma-associated protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL392048.9, BC040540.1 and L41870.1. This sequence is a reference standard in the RefSeqGene project. On Jun 9, 2006 this sequence version replaced NM_000321.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040540.1, SRR1660809.155247.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGCCCA AAACCCCCCG AAAAACGGCC GCCACCGCCG CCGCTGCCGC CGCGGAACCC 
CCGGCACCGC CGCCGCCGCC CCCTCCTGAG GAGGACCCAG AGCAGGACAG CGGCCCGGAG 
GACCTGCCTC TCGTCAGGCT TGAGTTTGAA GAAACAGAAG AACCTGATTT TACTGCATTA 
TGTCAGAAAT TAAAGATACC AGATCATGTC AGAGAGAGAG CTTGGTTAAC TTGGGAGAAA 
GTTTCATCTG TGGATGGAGT ATTGGGAGGT TATATTCAAA AGAAAAAGGA ACTGTGGGGA 
ATCTGTATCT TTATTGCAGC AGTTGACCTA GATGAGATGT CGTTCACTTT TACTGAGCTA 
CAGAAAAACA TAGAAATCAG TGTCCATAAA TTCTTTAACT TACTAAAAGA AATTGATACC 
AGTACCAAAG TTGATAATGC TATGTCAAGA CTGTTGAAGA AGTATGATGT ATTGTTTGCA 
CTCTTCAGCA AATTGGAAAG GACATGTGAA CTTATATATT TGACACAACC CAGCAGTTCG 
ATATCTACTG AAATAAATTC TGCATTGGTG CTAAAAGTTT CTTGGATCAC ATTTTTATTA 
GCTAAAGGGG AAGTATTACA AATGGAAGAT GATCTGGTGA TTTCATTTCA GTTAATGCTA 
TGTGTCCTTG ACTATTTTAT TAAACTCTCA CCTCCCATGT TGCTCAAAGA ACCATATAAA 
ACAGCTGTTA TACCCATTAA TGGTTCACCT CGAACACCCA GGCGAGGTCA GAACAGGAGT 
GCACGGATAG CAAAACAACT AGAAAATGAT ACAAGAATTA TTGAAGTTCT CTGTAAAGAA 
CATGAATGTA ATATAGATGA GGTGAAAAAT GTTTATTTCA AAAATTTTAT ACCTTTTATG 
AATTCTCTTG GACTTGTAAC ATCTAATGGA CTTCCAGAGG TTGAAAATCT TTCTAAACGA 
TACGAAGAAA TTTATCTTAA AAATAAAGAT CTAGATGCAA GATTATTTTT GGATCATGAT 
AAAACTCTTC AGACTGATTC TATAGACAGT TTTGAAACAC AGAGAACACC ACGAAAAAGT 
AACCTTGATG AAGAGGTGAA TGTAATTCCT CCACACACTC CAGTTAGGAC TGTTATGAAC 
ACTATCCAAC AATTAATGAT GATTTTAAAT TCAGCAAGTG ATCAACCTTC AGAAAATCTG 
ATTTCCTATT TTAACAACTG CACAGTGAAT CCAAAAGAAA GTATACTGAA AAGAGTGAAG 
GATATAGGAT ACATCTTTAA AGAGAAATTT GCTAAAGCTG TGGGACAGGG TTGTGTCGAA 
ATTGGATCAC AGCGATACAA ACTTGGAGTT CGCTTGTATT ACCGAGTAAT GGAATCCATG 
CTTAAATCAG AAGAAGAACG ATTATCCATT CAAAATTTTA GCAAACTTCT GAATGACAAC 
ATTTTTCATA TGTCTTTATT GGCGTGCGCT CTTGAGGTTG TAATGGCCAC ATATAGCAGA 
AGTACATCTC AGAATCTTGA TTCTGGAACA GATTTGTCTT TCCCATGGAT TCTGAATGTG 
CTTAATTTAA AAGCCTTTGA TTTTTACAAA GTGATCGAAA GTTTTATCAA AGCAGAAGGC 
AACTTGACAA GAGAAATGAT AAAACATTTA GAACGATGTG AACATCGAAT CATGGAATCC 
CTTGCATGGC TCTCAGATTC ACCTTTATTT GATCTTATTA AACAATCAAA GGACCGAGAA 
GGACCAACTG ATCACCTTGA ATCTGCTTGT CCTCTTAATC TTCCTCTCCA GAATAATCAC 
ACTGCAGCAG ATATGTATCT TTCTCCTGTA AGATCTCCAA AGAAAAAAGG TTCAACTACG 
CGTGTAAATT CTACTGCAAA TGCAGAGACA CAAGCAACCT CAGCCTTCCA GACCCAGAAG 
CCATTGAAAT CTACCTCTCT TTCACTGTTT TATAAAAAAG TGTATCGGCT AGCCTATCTC 
CGGCTAAATA CACTTTGTGA ACGCCTTCTG TCTGAGCACC CAGAATTAGA ACATATCATC 
TGGACCCTTT TCCAGCACAC CCTGCAGAAT GAGTATGAAC TCATGAGAGA CAGGCATTTG 
GACCAAATTA TGATGTGTTC CATGTATGGC ATATGCAAAG TGAAGAATAT AGACCTTAAA 
TTCAAAATCA TTGTAACAGC ATACAAGGAT CTTCCTCATG CTGTTCAGGA GACATTCAAA 
CGTGTTTTGA TCAAAGAAGA GGAGTATGAT TCTATTATAG TATTCTATAA CTCGGTCTTC 
ATGCAGAGAC TGAAAACAAA TATTTTGCAG TATGCTTCCA CCAGGCCCCC TACCTTGTCA 
CCAATACCTC ACATTCCTCG AAGCCCTTAC AAGTTTCCTA GTTCACCCTT ACGGATTCCT 
GGAGGGAACA TCTATATTTC ACCCCTGAAG AGTCCATATA AAATTTCAGA AGGTCTGCCA 
ACACCAACAA AAATGACTCC AAGATCAAGA ATCTTAGTAT CAATTGGTGA ATCATTCGGG 
ACTTCTGAGA AGTTCCAGAA AATAAATCAG ATGGTATGTA ACAGCGACCG TGTGCTCAAA 
AGAAGTGCTG AAGGAAGCAA CCCTCCTAAA CCACTGAAAA AACTACGCTT TGATATTGAA 
GGATCAGATG AAGCAGATGG AAGTAAACAT CTCCCAGGAG AGTCCAAATT TCAGCAGAAA 
CTGGCAGAAA TGACTTCTAC TCGAACACGA ATGCAAAAGC AGAAAATGAA TGATAGCATG 
GATACCTCAA ACAAGGAAGA GAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000312.2
CDS	167..2953
Translation	MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPLVRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFALFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQLMLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQKMNDSMDTSNKEEK

Target ORF information:

RefSeq Version	NM_000321.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RB transcriptional corepressor 1 (RB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000321.2

ORF Insert Sequence:

ATGCCGCCCA AAACCCCCCG AAAAACGGCC GCCACCGCCG CCGCTGCCGC CGCGGAACCC 
CCGGCACCGC CGCCGCCGCC CCCTCCTGAG GAGGACCCAG AGCAGGACAG CGGCCCGGAG 
GACCTGCCTC TCGTCAGGCT TGAGTTTGAA GAAACAGAAG AACCTGATTT TACTGCATTA 
TGTCAGAAAT TAAAGATACC AGATCATGTC AGAGAGAGAG CTTGGTTAAC TTGGGAGAAA 
GTTTCATCTG TGGATGGAGT ATTGGGAGGT TATATTCAAA AGAAAAAGGA ACTGTGGGGA 
ATCTGTATCT TTATTGCAGC AGTTGACCTA GATGAGATGT CGTTCACTTT TACTGAGCTA 
CAGAAAAACA TAGAAATCAG TGTCCATAAA TTCTTTAACT TACTAAAAGA AATTGATACC 
AGTACCAAAG TTGATAATGC TATGTCAAGA CTGTTGAAGA AGTATGATGT ATTGTTTGCA 
CTCTTCAGCA AATTGGAAAG GACATGTGAA CTTATATATT TGACACAACC CAGCAGTTCG 
ATATCTACTG AAATAAATTC TGCATTGGTG CTAAAAGTTT CTTGGATCAC ATTTTTATTA 
GCTAAAGGGG AAGTATTACA AATGGAAGAT GATCTGGTGA TTTCATTTCA GTTAATGCTA 
TGTGTCCTTG ACTATTTTAT TAAACTCTCA CCTCCCATGT TGCTCAAAGA ACCATATAAA 
ACAGCTGTTA TACCCATTAA TGGTTCACCT CGAACACCCA GGCGAGGTCA GAACAGGAGT 
GCACGGATAG CAAAACAACT AGAAAATGAT ACAAGAATTA TTGAAGTTCT CTGTAAAGAA 
CATGAATGTA ATATAGATGA GGTGAAAAAT GTTTATTTCA AAAATTTTAT ACCTTTTATG 
AATTCTCTTG GACTTGTAAC ATCTAATGGA CTTCCAGAGG TTGAAAATCT TTCTAAACGA 
TACGAAGAAA TTTATCTTAA AAATAAAGAT CTAGATGCAA GATTATTTTT GGATCATGAT 
AAAACTCTTC AGACTGATTC TATAGACAGT TTTGAAACAC AGAGAACACC ACGAAAAAGT 
AACCTTGATG AAGAGGTGAA TGTAATTCCT CCACACACTC CAGTTAGGAC TGTTATGAAC 
ACTATCCAAC AATTAATGAT GATTTTAAAT TCAGCAAGTG ATCAACCTTC AGAAAATCTG 
ATTTCCTATT TTAACAACTG CACAGTGAAT CCAAAAGAAA GTATACTGAA AAGAGTGAAG 
GATATAGGAT ACATCTTTAA AGAGAAATTT GCTAAAGCTG TGGGACAGGG TTGTGTCGAA 
ATTGGATCAC AGCGATACAA ACTTGGAGTT CGCTTGTATT ACCGAGTAAT GGAATCCATG 
CTTAAATCAG AAGAAGAACG ATTATCCATT CAAAATTTTA GCAAACTTCT GAATGACAAC 
ATTTTTCATA TGTCTTTATT GGCGTGCGCT CTTGAGGTTG TAATGGCCAC ATATAGCAGA 
AGTACATCTC AGAATCTTGA TTCTGGAACA GATTTGTCTT TCCCATGGAT TCTGAATGTG 
CTTAATTTAA AAGCCTTTGA TTTTTACAAA GTGATCGAAA GTTTTATCAA AGCAGAAGGC 
AACTTGACAA GAGAAATGAT AAAACATTTA GAACGATGTG AACATCGAAT CATGGAATCC 
CTTGCATGGC TCTCAGATTC ACCTTTATTT GATCTTATTA AACAATCAAA GGACCGAGAA 
GGACCAACTG ATCACCTTGA ATCTGCTTGT CCTCTTAATC TTCCTCTCCA GAATAATCAC 
ACTGCAGCAG ATATGTATCT TTCTCCTGTA AGATCTCCAA AGAAAAAAGG TTCAACTACG 
CGTGTAAATT CTACTGCAAA TGCAGAGACA CAAGCAACCT CAGCCTTCCA GACCCAGAAG 
CCATTGAAAT CTACCTCTCT TTCACTGTTT TATAAAAAAG TGTATCGGCT AGCCTATCTC 
CGGCTAAATA CACTTTGTGA ACGCCTTCTG TCTGAGCACC CAGAATTAGA ACATATCATC 
TGGACCCTTT TCCAGCACAC CCTGCAGAAT GAGTATGAAC TCATGAGAGA CAGGCATTTG 
GACCAAATTA TGATGTGTTC CATGTATGGC ATATGCAAAG TGAAGAATAT AGACCTTAAA 
TTCAAAATCA TTGTAACAGC ATACAAGGAT CTTCCTCATG CTGTTCAGGA GACATTCAAA 
CGTGTTTTGA TCAAAGAAGA GGAGTATGAT TCTATTATAG TATTCTATAA CTCGGTCTTC 
ATGCAGAGAC TGAAAACAAA TATTTTGCAG TATGCTTCCA CCAGGCCCCC TACCTTGTCA 
CCAATACCTC ACATTCCTCG AAGCCCTTAC AAGTTTCCTA GTTCACCCTT ACGGATTCCT 
GGAGGGAACA TCTATATTTC ACCCCTGAAG AGTCCATATA AAATTTCAGA AGGTCTGCCA 
ACACCAACAA AAATGACTCC AAGATCAAGA ATCTTAGTAT CAATTGGTGA ATCATTCGGG 
ACTTCTGAGA AGTTCCAGAA AATAAATCAG ATGGTATGTA ACAGCGACCG TGTGCTCAAA 
AGAAGTGCTG AAGGAAGCAA CCCTCCTAAA CCACTGAAAA AACTACGCTT TGATATTGAA 
GGATCAGATG AAGCAGATGG AAGTAAACAT CTCCCAGGAG AGTCCAAATT TCAGCAGAAA 
CTGGCAGAAA TGACTTCTAC TCGAACACGA ATGCAAAAGC AGAAAATGAA TGATAGCATG 
GATACCTCAA ACAAGGAAGA GAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RB1 ( XM_011535171.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533473.1 Homo sapiens RB transcriptional corepressor 1 (RB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu60211
Clone ID Related Accession (Same CDS sequence)	XM_011535171.2
Accession Version	XM_011535171.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	retinoblastoma-associated protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535171.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGTT ATATTCAAAA GAAAAAGGAA CTGTGGGGAA TCTGTATCTT TATTGCAGCA 
GTTGACCTAG ATGAGATGTC GTTCACTTTT ACTGAGCTAC AGAAAAACAT AGAAATCAGT 
GTCCATAAAT TCTTTAACTT ACTAAAAGAA ATTGATACCA GTACCAAAGT TGATAATGCT 
ATGTCAAGAC TGTTGAAGAA GTATGATGTA TTGTTTGCAC TCTTCAGCAA ATTGGAAAGG 
ACATGTGAAC TTATATATTT GACACAACCC AGCAGTTCGA TATCTACTGA AATAAATTCT 
GCATTGGTGC TAAAAGTTTC TTGGATCACA TTTTTATTAG CTAAAGGGGA AGTATTACAA 
ATGGAAGATG ATCTGGTGAT TTCATTTCAG TTAATGCTAT GTGTCCTTGA CTATTTTATT 
AAACTCTCAC CTCCCATGTT GCTCAAAGAA CCATATAAAA CAGCTGTTAT ACCCATTAAT 
GGTTCACCTC GAACACCCAG GCGAGGTCAG AACAGGAGTG CACGGATAGC AAAACAACTA 
GAAAATGATA CAAGAATTAT TGAAGTTCTC TGTAAAGAAC ATGAATGTAA TATAGATGAG 
GTGAAAAATG TTTATTTCAA AAATTTTATA CCTTTTATGA ATTCTCTTGG ACTTGTAACA 
TCTAATGGAC TTCCAGAGGT TGAAAATCTT TCTAAACGAT ACGAAGAAAT TTATCTTAAA 
AATAAAGATC TAGATGCAAG ATTATTTTTG GATCATGATA AAACTCTTCA GACTGATTCT 
ATAGACAGTT TTGAAACACA GAGAACACCA CGAAAAAGTA ACCTTGATGA AGAGGTGAAT 
GTAATTCCTC CACACACTCC AGTTAGGACT GTTATGAACA CTATCCAACA ATTAATGATG 
ATTTTAAATT CAGCAAGTGA TCAACCTTCA GAAAATCTGA TTTCCTATTT TAACAACTGC 
ACAGTGAATC CAAAAGAAAG TATACTGAAA AGAGTGAAGG ATATAGGATA CATCTTTAAA 
GAGAAATTTG CTAAAGCTGT GGGACAGGGT TGTGTCGAAA TTGGATCACA GCGATACAAA 
CTTGGAGTTC GCTTGTATTA CCGAGTAATG GAATCCATGC TTAAATCAGA AGAAGAACGA 
TTATCCATTC AAAATTTTAG CAAACTTCTG AATGACAACA TTTTTCATAT GTCTTTATTG 
GCGTGCGCTC TTGAGGTTGT AATGGCCACA TATAGCAGAA GTACATCTCA GAATCTTGAT 
TCTGGAACAG ATTTGTCTTT CCCATGGATT CTGAATGTGC TTAATTTAAA AGCCTTTGAT 
TTTTACAAAG TGATCGAAAG TTTTATCAAA GCAGAAGGCA ACTTGACAAG AGAAATGATA 
AAACATTTAG AACGATGTGA ACATCGAATC ATGGAATCCC TTGCATGGCT CTCAGATTCA 
CCTTTATTTG ATCTTATTAA ACAATCAAAG GACCGAGAAG GACCAACTGA TCACCTTGAA 
TCTGCTTGTC CTCTTAATCT TCCTCTCCAG AATAATCACA CTGCAGCAGA TATGTATCTT 
TCTCCTGTAA GATCTCCAAA GAAAAAAGGT TCAACTACGC GTGTAAATTC TACTGCAAAT 
GCAGAGACAC AAGCAACCTC AGCCTTCCAG ACCCAGAAGC CATTGAAATC TACCTCTCTT 
TCACTGTTTT ATAAAAAAGT GTATCGGCTA GCCTATCTCC GGCTAAATAC ACTTTGTGAA 
CGCCTTCTGT CTGAGCACCC AGAATTAGAA CATATCATCT GGACCCTTTT CCAGCACACC 
CTGCAGAATG AGTATGAACT CATGAGAGAC AGGCATTTGG ACCAAATTAT GATGTGTTCC 
ATGTATGGCA TATGCAAAGT GAAGAATATA GACCTTAAAT TCAAAATCAT TGTAACAGCA 
TACAAGGATC TTCCTCATGC TGTTCAGGAG ACATTCAAAC GTGTTTTGAT CAAAGAAGAG 
GAGTATGATT CTATTATAGT ATTCTATAAC TCGGTCTTCA TGCAGAGACT GAAAACAAAT 
ATTTTGCAGT ATGCTTCCAC CAGGCCCCCT ACCTTGTCAC CAATACCTCA CATTCCTCGA 
AGCCCTTACA AGTTTCCTAG TTCACCCTTA CGGATTCCTG GAGGGAACAT CTATATTTCA 
CCCCTGAAGA GTCCATATAA AATTTCAGAA GGTCTGCCAA CACCAACAAA AATGACTCCA 
AGATCAAGAA TCTTAGTATC AATTGGTGAA TCATTCGGGA CTTCTGAGAA GTTCCAGAAA 
ATAAATCAGA TGGTATGTAA CAGCGACCGT GTGCTCAAAA GAAGTGCTGA AGGAAGCAAC 
CCTCCTAAAC CACTGAAAAA ACTACGCTTT GATATTGAAG GATCAGATGA AGCAGATGGA 
AGTAAACATC TCCCAGGAGA GTCCAAATTT CAGCAGAAAC TGGCAGAAAT GACTTCTACT 
CGAACACGAA TGCAAAAGCA GAAAATGAAT GATAGCATGG ATACCTCAAA CAAGGAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533473.1
CDS	17..2542
Translation	MGGYIQKKKELWGICIFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFALFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQLMLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQKMNDSMDTSNKEEK

Target ORF information:

RefSeq Version	XM_011535171.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RB transcriptional corepressor 1 (RB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535171.2

ORF Insert Sequence:

ATGGGAGGTT ATATTCAAAA GAAAAAGGAA CTGTGGGGAA TCTGTATCTT TATTGCAGCA 
GTTGACCTAG ATGAGATGTC GTTCACTTTT ACTGAGCTAC AGAAAAACAT AGAAATCAGT 
GTCCATAAAT TCTTTAACTT ACTAAAAGAA ATTGATACCA GTACCAAAGT TGATAATGCT 
ATGTCAAGAC TGTTGAAGAA GTATGATGTA TTGTTTGCAC TCTTCAGCAA ATTGGAAAGG 
ACATGTGAAC TTATATATTT GACACAACCC AGCAGTTCGA TATCTACTGA AATAAATTCT 
GCATTGGTGC TAAAAGTTTC TTGGATCACA TTTTTATTAG CTAAAGGGGA AGTATTACAA 
ATGGAAGATG ATCTGGTGAT TTCATTTCAG TTAATGCTAT GTGTCCTTGA CTATTTTATT 
AAACTCTCAC CTCCCATGTT GCTCAAAGAA CCATATAAAA CAGCTGTTAT ACCCATTAAT 
GGTTCACCTC GAACACCCAG GCGAGGTCAG AACAGGAGTG CACGGATAGC AAAACAACTA 
GAAAATGATA CAAGAATTAT TGAAGTTCTC TGTAAAGAAC ATGAATGTAA TATAGATGAG 
GTGAAAAATG TTTATTTCAA AAATTTTATA CCTTTTATGA ATTCTCTTGG ACTTGTAACA 
TCTAATGGAC TTCCAGAGGT TGAAAATCTT TCTAAACGAT ACGAAGAAAT TTATCTTAAA 
AATAAAGATC TAGATGCAAG ATTATTTTTG GATCATGATA AAACTCTTCA GACTGATTCT 
ATAGACAGTT TTGAAACACA GAGAACACCA CGAAAAAGTA ACCTTGATGA AGAGGTGAAT 
GTAATTCCTC CACACACTCC AGTTAGGACT GTTATGAACA CTATCCAACA ATTAATGATG 
ATTTTAAATT CAGCAAGTGA TCAACCTTCA GAAAATCTGA TTTCCTATTT TAACAACTGC 
ACAGTGAATC CAAAAGAAAG TATACTGAAA AGAGTGAAGG ATATAGGATA CATCTTTAAA 
GAGAAATTTG CTAAAGCTGT GGGACAGGGT TGTGTCGAAA TTGGATCACA GCGATACAAA 
CTTGGAGTTC GCTTGTATTA CCGAGTAATG GAATCCATGC TTAAATCAGA AGAAGAACGA 
TTATCCATTC AAAATTTTAG CAAACTTCTG AATGACAACA TTTTTCATAT GTCTTTATTG 
GCGTGCGCTC TTGAGGTTGT AATGGCCACA TATAGCAGAA GTACATCTCA GAATCTTGAT 
TCTGGAACAG ATTTGTCTTT CCCATGGATT CTGAATGTGC TTAATTTAAA AGCCTTTGAT 
TTTTACAAAG TGATCGAAAG TTTTATCAAA GCAGAAGGCA ACTTGACAAG AGAAATGATA 
AAACATTTAG AACGATGTGA ACATCGAATC ATGGAATCCC TTGCATGGCT CTCAGATTCA 
CCTTTATTTG ATCTTATTAA ACAATCAAAG GACCGAGAAG GACCAACTGA TCACCTTGAA 
TCTGCTTGTC CTCTTAATCT TCCTCTCCAG AATAATCACA CTGCAGCAGA TATGTATCTT 
TCTCCTGTAA GATCTCCAAA GAAAAAAGGT TCAACTACGC GTGTAAATTC TACTGCAAAT 
GCAGAGACAC AAGCAACCTC AGCCTTCCAG ACCCAGAAGC CATTGAAATC TACCTCTCTT 
TCACTGTTTT ATAAAAAAGT GTATCGGCTA GCCTATCTCC GGCTAAATAC ACTTTGTGAA 
CGCCTTCTGT CTGAGCACCC AGAATTAGAA CATATCATCT GGACCCTTTT CCAGCACACC 
CTGCAGAATG AGTATGAACT CATGAGAGAC AGGCATTTGG ACCAAATTAT GATGTGTTCC 
ATGTATGGCA TATGCAAAGT GAAGAATATA GACCTTAAAT TCAAAATCAT TGTAACAGCA 
TACAAGGATC TTCCTCATGC TGTTCAGGAG ACATTCAAAC GTGTTTTGAT CAAAGAAGAG 
GAGTATGATT CTATTATAGT ATTCTATAAC TCGGTCTTCA TGCAGAGACT GAAAACAAAT 
ATTTTGCAGT ATGCTTCCAC CAGGCCCCCT ACCTTGTCAC CAATACCTCA CATTCCTCGA 
AGCCCTTACA AGTTTCCTAG TTCACCCTTA CGGATTCCTG GAGGGAACAT CTATATTTCA 
CCCCTGAAGA GTCCATATAA AATTTCAGAA GGTCTGCCAA CACCAACAAA AATGACTCCA 
AGATCAAGAA TCTTAGTATC AATTGGTGAA TCATTCGGGA CTTCTGAGAA GTTCCAGAAA 
ATAAATCAGA TGGTATGTAA CAGCGACCGT GTGCTCAAAA GAAGTGCTGA AGGAAGCAAC 
CCTCCTAAAC CACTGAAAAA ACTACGCTTT GATATTGAAG GATCAGATGA AGCAGATGGA 
AGTAAACATC TCCCAGGAGA GTCCAAATTT CAGCAGAAAC TGGCAGAAAT GACTTCTACT 
CGAACACGAA TGCAAAAGCA GAAAATGAAT GATAGCATGG ATACCTCAAA CAAGGAAGAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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RB1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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