PLEKHG4 cDNA ORF clone, Gallus gallus(chicken)

The following PLEKHG4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLEKHG4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa40846 XM_004944099.3
Latest version!
Gallus gallus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa40847 XM_004944100.3
Latest version!
Gallus gallus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa40848 XM_004944101.3
Latest version!
Gallus gallus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa40848 XM_004944102.3
Latest version!
Gallus gallus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa40849 XM_003641900.4
Latest version!
Gallus gallus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa40850 XM_015279197.1
Latest version!
Gallus gallus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa40851 XM_015279198.2
Latest version!
Gallus gallus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
View Old Accession Versions >>
OGa40851 XM_015279198.1 Gallus gallus pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa40846 XM_004944099.2 Gallus gallus pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa40847 XM_004944100.2 Gallus gallus pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa40848 XM_004944101.2 Gallus gallus pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa40849 XM_003641900.3 Gallus gallus pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa40848 XM_004944102.2 Gallus gallus pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa40846
    Clone ID Related Accession (Same CDS sequence) XM_004944099.3 , XM_004944099.2
    Accession Version XM_004944099.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product puratrophin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006098.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004944099.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGGTG TGATTCCTTC CCAGGGCGTC TGTGCCGCAA AAGAAATCCG CATGCAGGAG
    ATGGTCTCCA TGGGTGTGGG CAACAAGCCA TTCCTGGATA TCAAACCCAG TGAGGCTGCT
    ATCAATGACC GTGCTATTGA CTACATCATG AAAGGCAGAG CTGCCAGGAC CAGAGCATCA
    ATTGCGGTGT CTCTCTTTGA CCATTCCAAC CCCTACAAGA GGACGCAGGC ACAGCTTTCT
    GCTGGCAGCA CCCCTGCTGC ATATGGCCCA TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC
    CTGCACATGT ACCTGGACCA GGCACTGCTG CCTGGTGTCC TGGCACCCCG GCGCCCCTTT
    GATGTCAGCC CCTGCATTGA GGAAGATGAG CTGGAGCATG AGACGAGCAG CCAGCCATCA
    CTGACCACAG AGAGCTCCGA GTCATCACAC TGCATGTCAG GCAGCGGCTC CAGCGGCTCC
    GACAGTGGCT GTGTCTCCAG CATCCCACAA GAAAGCTTCT GTGAGGACAT GGGTTCACCT
    CCCTTCATGC CCCTGGGCTC ACAACACAGC TCTGCGATGG AGGGCAAACC CCGGTTCACG
    AACAGCCAGT ACATTTCTGC AGTAAGTGCA AGCAGCCAAA GCACTCTCTT CGCTCTGCCC
    GTTCCCACCA CTGCCTCCCC AGTTCAAGGC ACTGCCTCTC ACGTGGGGAC TGAAGATGCT
    GCTTTTGGGC TGGGAATTGC TGTGGGGAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004944156.2
    CDS57..6629
    Translation

    Target ORF information:

    RefSeq Version XM_004944099.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004944099.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGGTG TGATTCCTTC CCAGGGCGTC TGTGCCGCAA AAGAAATCCG CATGCAGGAG
    ATGGTCTCCA TGGGTGTGGG CAACAAGCCA TTCCTGGATA TCAAACCCAG TGAGGCTGCT
    ATCAATGACC GTGCTATTGA CTACATCATG AAAGGCAGAG CTGCCAGGAC CAGAGCATCA
    ATTGCGGTGT CTCTCTTTGA CCATTCCAAC CCCTACAAGA GGACGCAGGC ACAGCTTTCT
    GCTGGCAGCA CCCCTGCTGC ATATGGCCCA TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC
    CTGCACATGT ACCTGGACCA GGCACTGCTG CCTGGTGTCC TGGCACCCCG GCGCCCCTTT
    GATGTCAGCC CCTGCATTGA GGAAGATGAG CTGGAGCATG AGACGAGCAG CCAGCCATCA
    CTGACCACAG AGAGCTCCGA GTCATCACAC TGCATGTCAG GCAGCGGCTC CAGCGGCTCC
    GACAGTGGCT GTGTCTCCAG CATCCCACAA GAAAGCTTCT GTGAGGACAT GGGTTCACCT
    CCCTTCATGC CCCTGGGCTC ACAACACAGC TCTGCGATGG AGGGCAAACC CCGGTTCACG
    AACAGCCAGT ACATTTCTGC AGTAAGTGCA AGCAGCCAAA GCACTCTCTT CGCTCTGCCC
    GTTCCCACCA CTGCCTCCCC AGTTCAAGGC ACTGCCTCTC ACGTGGGGAC TGAAGATGCT
    GCTTTTGGGC TGGGAATTGC TGTGGGGAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40846
    Clone ID Related Accession (Same CDS sequence) XM_004944099.3 , XM_004944099.2
    Accession Version XM_004944099.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product puratrophin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763868.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004944099.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGGTG TGATTCCTTC CCAGGGCGTC TGTGCCGCAA AAGAAATCCG CATGCAGGAG
    ATGGTCTCCA TGGGTGTGGG CAACAAGCCA TTCCTGGATA TCAAACCCAG TGAGGCTGCT
    ATCAATGACC GTGCTATTGA CTACATCATG AAAGGCAGAG CTGCCAGGAC CAGAGCATCA
    ATTGCGGTGT CTCTCTTTGA CCATTCCAAC CCCTACAAGA GGACGCAGGC ACAGCTTTCT
    GCTGGCAGCA CCCCTGCTGC ATATGGCCCA TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC
    CTGCACATGT ACCTGGACCA GGCACTGCTG CCTGGTGTCC TGGCACCCCG GCGCCCCTTT
    GATGTCAGCC CCTGCATTGA GGAAGATGAG CTGGAGCATG AGACGAGCAG CCAGCCATCA
    CTGACCACAG AGAGCTCCGA GTCATCACAC TGCATGTCAG GCAGCGGCTC CAGCGGCTCC
    GACAGTGGCT GTGTCTCCAG CATCCCACAA GAAAGCTTCT GTGAGGACAT GGGTTCACCT
    CCCTTCATGC CCCTGGGCTC ACAACACAGC TCTGCGATGG AGGGCAAACC CCGGTTCACG
    AACAGCCAGT ACATTTCTGC AGTAAGTGCA AGCAGCCAAA GCACTCTCTT CGCTCTGCCC
    GTTCCCACCA CTGCCTCCCC AGTTCAAGGC ACTGCCTCTC ACGTGGGGAC TGAAGATGCT
    GCTTTTGGGC TGGGAATTGC TGTGGGGAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004944156.2
    CDS24..6596
    Translation

    Target ORF information:

    RefSeq Version XM_004944099.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004944099.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGGTG TGATTCCTTC CCAGGGCGTC TGTGCCGCAA AAGAAATCCG CATGCAGGAG
    ATGGTCTCCA TGGGTGTGGG CAACAAGCCA TTCCTGGATA TCAAACCCAG TGAGGCTGCT
    ATCAATGACC GTGCTATTGA CTACATCATG AAAGGCAGAG CTGCCAGGAC CAGAGCATCA
    ATTGCGGTGT CTCTCTTTGA CCATTCCAAC CCCTACAAGA GGACGCAGGC ACAGCTTTCT
    GCTGGCAGCA CCCCTGCTGC ATATGGCCCA TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC
    CTGCACATGT ACCTGGACCA GGCACTGCTG CCTGGTGTCC TGGCACCCCG GCGCCCCTTT
    GATGTCAGCC CCTGCATTGA GGAAGATGAG CTGGAGCATG AGACGAGCAG CCAGCCATCA
    CTGACCACAG AGAGCTCCGA GTCATCACAC TGCATGTCAG GCAGCGGCTC CAGCGGCTCC
    GACAGTGGCT GTGTCTCCAG CATCCCACAA GAAAGCTTCT GTGAGGACAT GGGTTCACCT
    CCCTTCATGC CCCTGGGCTC ACAACACAGC TCTGCGATGG AGGGCAAACC CCGGTTCACG
    AACAGCCAGT ACATTTCTGC AGTAAGTGCA AGCAGCCAAA GCACTCTCTT CGCTCTGCCC
    GTTCCCACCA CTGCCTCCCC AGTTCAAGGC ACTGCCTCTC ACGTGGGGAC TGAAGATGCT
    GCTTTTGGGC TGGGAATTGC TGTGGGGAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40847
    Clone ID Related Accession (Same CDS sequence) XM_004944100.3 , XM_004944100.2
    Accession Version XM_004944100.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product puratrophin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006098.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004944100.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGAAA TCCGCATGCA GGAGATGGTC TCCATGGGTG TGGGCAACAA GCCATTCCTG
    GATATCAAAC CCAGTGAGGC TGCTATCAAT GACCGTGCTA TTGACTACAT CATGAAAGGC
    AGAGCTGCCA GGACCAGAGC ATCAATTGCG GTGTCTCTCT TTGACCATTC CAACCCCTAC
    AAGAGGACGC AGGCACAGCT TTCTGCTGGC AGCACCCCTG CTGCATATGG CCCATCCTCC
    TCCTCGCTGC TGGGGCCCCT CAACCTGCAC ATGTACCTGG ACCAGGCACT GCTGCCTGGT
    GTCCTGGCAC CCCGGCGCCC CTTTGATGTC AGCCCCTGCA TTGAGGAAGA TGAGCTGGAG
    CATGAGACGA GCAGCCAGCC ATCACTGACC ACAGAGAGCT CCGAGTCATC ACACTGCATG
    TCAGGCAGCG GCTCCAGCGG CTCCGACAGT GGCTGTGTCT CCAGCATCCC ACAAGAAAGC
    TTCTGTGAGG ACATGGGTTC ACCTCCCTTC ATGCCCCTGG GCTCACAACA CAGCTCTGCG
    ATGGAGGGCA AACCCCGGTT CACGAACAGC CAGTACATTT CTGCAGTAAG TGCAAGCAGC
    CAAAGCACTC TCTTCGCTCT GCCCGTTCCC ACCACTGCCT CCCCAGTTCA AGGCACTGCC
    TCTCACGTGG GGACTGAAGA TGCTGCTTTT GGGCTGGGAA TTGCTGTGGG GAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004944157.2
    CDS53..6589
    Translation

    Target ORF information:

    RefSeq Version XM_004944100.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004944100.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGAAA TCCGCATGCA GGAGATGGTC TCCATGGGTG TGGGCAACAA GCCATTCCTG
    GATATCAAAC CCAGTGAGGC TGCTATCAAT GACCGTGCTA TTGACTACAT CATGAAAGGC
    AGAGCTGCCA GGACCAGAGC ATCAATTGCG GTGTCTCTCT TTGACCATTC CAACCCCTAC
    AAGAGGACGC AGGCACAGCT TTCTGCTGGC AGCACCCCTG CTGCATATGG CCCATCCTCC
    TCCTCGCTGC TGGGGCCCCT CAACCTGCAC ATGTACCTGG ACCAGGCACT GCTGCCTGGT
    GTCCTGGCAC CCCGGCGCCC CTTTGATGTC AGCCCCTGCA TTGAGGAAGA TGAGCTGGAG
    CATGAGACGA GCAGCCAGCC ATCACTGACC ACAGAGAGCT CCGAGTCATC ACACTGCATG
    TCAGGCAGCG GCTCCAGCGG CTCCGACAGT GGCTGTGTCT CCAGCATCCC ACAAGAAAGC
    TTCTGTGAGG ACATGGGTTC ACCTCCCTTC ATGCCCCTGG GCTCACAACA CAGCTCTGCG
    ATGGAGGGCA AACCCCGGTT CACGAACAGC CAGTACATTT CTGCAGTAAG TGCAAGCAGC
    CAAAGCACTC TCTTCGCTCT GCCCGTTCCC ACCACTGCCT CCCCAGTTCA AGGCACTGCC
    TCTCACGTGG GGACTGAAGA TGCTGCTTTT GGGCTGGGAA TTGCTGTGGG GAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40847
    Clone ID Related Accession (Same CDS sequence) XM_004944100.3 , XM_004944100.2
    Accession Version XM_004944100.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product puratrophin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763868.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004944100.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGAAA TCCGCATGCA GGAGATGGTC TCCATGGGTG TGGGCAACAA GCCATTCCTG
    GATATCAAAC CCAGTGAGGC TGCTATCAAT GACCGTGCTA TTGACTACAT CATGAAAGGC
    AGAGCTGCCA GGACCAGAGC ATCAATTGCG GTGTCTCTCT TTGACCATTC CAACCCCTAC
    AAGAGGACGC AGGCACAGCT TTCTGCTGGC AGCACCCCTG CTGCATATGG CCCATCCTCC
    TCCTCGCTGC TGGGGCCCCT CAACCTGCAC ATGTACCTGG ACCAGGCACT GCTGCCTGGT
    GTCCTGGCAC CCCGGCGCCC CTTTGATGTC AGCCCCTGCA TTGAGGAAGA TGAGCTGGAG
    CATGAGACGA GCAGCCAGCC ATCACTGACC ACAGAGAGCT CCGAGTCATC ACACTGCATG
    TCAGGCAGCG GCTCCAGCGG CTCCGACAGT GGCTGTGTCT CCAGCATCCC ACAAGAAAGC
    TTCTGTGAGG ACATGGGTTC ACCTCCCTTC ATGCCCCTGG GCTCACAACA CAGCTCTGCG
    ATGGAGGGCA AACCCCGGTT CACGAACAGC CAGTACATTT CTGCAGTAAG TGCAAGCAGC
    CAAAGCACTC TCTTCGCTCT GCCCGTTCCC ACCACTGCCT CCCCAGTTCA AGGCACTGCC
    TCTCACGTGG GGACTGAAGA TGCTGCTTTT GGGCTGGGAA TTGCTGTGGG GAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004944157.2
    CDS21..6557
    Translation

    Target ORF information:

    RefSeq Version XM_004944100.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004944100.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
    3481
    3541
    3601
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    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGAAA TCCGCATGCA GGAGATGGTC TCCATGGGTG TGGGCAACAA GCCATTCCTG
    GATATCAAAC CCAGTGAGGC TGCTATCAAT GACCGTGCTA TTGACTACAT CATGAAAGGC
    AGAGCTGCCA GGACCAGAGC ATCAATTGCG GTGTCTCTCT TTGACCATTC CAACCCCTAC
    AAGAGGACGC AGGCACAGCT TTCTGCTGGC AGCACCCCTG CTGCATATGG CCCATCCTCC
    TCCTCGCTGC TGGGGCCCCT CAACCTGCAC ATGTACCTGG ACCAGGCACT GCTGCCTGGT
    GTCCTGGCAC CCCGGCGCCC CTTTGATGTC AGCCCCTGCA TTGAGGAAGA TGAGCTGGAG
    CATGAGACGA GCAGCCAGCC ATCACTGACC ACAGAGAGCT CCGAGTCATC ACACTGCATG
    TCAGGCAGCG GCTCCAGCGG CTCCGACAGT GGCTGTGTCT CCAGCATCCC ACAAGAAAGC
    TTCTGTGAGG ACATGGGTTC ACCTCCCTTC ATGCCCCTGG GCTCACAACA CAGCTCTGCG
    ATGGAGGGCA AACCCCGGTT CACGAACAGC CAGTACATTT CTGCAGTAAG TGCAAGCAGC
    CAAAGCACTC TCTTCGCTCT GCCCGTTCCC ACCACTGCCT CCCCAGTTCA AGGCACTGCC
    TCTCACGTGG GGACTGAAGA TGCTGCTTTT GGGCTGGGAA TTGCTGTGGG GAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40848
    Clone ID Related Accession (Same CDS sequence) XM_004944101.2 , XM_004944101.3 , XM_004944102.2 , XM_004944102.3
    Accession Version XM_004944101.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product puratrophin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763868.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004944101.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
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    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGGTG TGATTCCTTC CCAGGGCGTC TGTGCCGCAA AAGAAATCCG CATGCAGGAG
    ATGGTCTCCA TGGGTGTGGG CAACAAGCCA TTCCTGGATA TCAAACCCAG TGAGGCTGCT
    ATCAATGACC GTGCTATTGA CTACATCATG AAAGGCAGAG CTGCCAGGAC CAGAGCATCA
    ATTGCGGTGT CTCTCTTTGA CCATTCCAAC CCCTACAAGA GGACGCAGGC ACAGCTTTCT
    GCTGGCAGCA CCCCTGCTGC ATATGGCCCA TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC
    CTGCACATGT ACCTGGACCA GGCACTGCTG CCTGGTGTCC TGGCACCCCG GCGCCCCTTT
    GATGTCAGCC CCTGCATTGA GGAAGATGAG CTGGAGCATG AGACGAGCAG CCAGCCATCA
    CTGACCACAG AGAGCTCCGA GTCATCACAC TGCATGTCAG GCAGCGGCTC CAGCGGCTCC
    GACAGTGGCT GTGTCTCCAG CATCCCACAA GAAAGCTTCT GTGAGGACAT GGGTTCACCT
    CCCTTCATGC CCCTGGGCTC ACAACACAGC TCTGCGATGG AGGGCAAACC CCGGTTCACG
    AACAGCCAGT ACATTTCTGC AAAAGCAGGC CAGGCCACCA TAAGTCCCTC GACAATAGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004944158.2
    CDS24..6506
    Translation

    Target ORF information:

    RefSeq Version XM_004944101.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004944101.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGGTG TGATTCCTTC CCAGGGCGTC TGTGCCGCAA AAGAAATCCG CATGCAGGAG
    ATGGTCTCCA TGGGTGTGGG CAACAAGCCA TTCCTGGATA TCAAACCCAG TGAGGCTGCT
    ATCAATGACC GTGCTATTGA CTACATCATG AAAGGCAGAG CTGCCAGGAC CAGAGCATCA
    ATTGCGGTGT CTCTCTTTGA CCATTCCAAC CCCTACAAGA GGACGCAGGC ACAGCTTTCT
    GCTGGCAGCA CCCCTGCTGC ATATGGCCCA TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC
    CTGCACATGT ACCTGGACCA GGCACTGCTG CCTGGTGTCC TGGCACCCCG GCGCCCCTTT
    GATGTCAGCC CCTGCATTGA GGAAGATGAG CTGGAGCATG AGACGAGCAG CCAGCCATCA
    CTGACCACAG AGAGCTCCGA GTCATCACAC TGCATGTCAG GCAGCGGCTC CAGCGGCTCC
    GACAGTGGCT GTGTCTCCAG CATCCCACAA GAAAGCTTCT GTGAGGACAT GGGTTCACCT
    CCCTTCATGC CCCTGGGCTC ACAACACAGC TCTGCGATGG AGGGCAAACC CCGGTTCACG
    AACAGCCAGT ACATTTCTGC AAAAGCAGGC CAGGCCACCA TAAGTCCCTC GACAATAGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40848
    Clone ID Related Accession (Same CDS sequence) XM_004944101.2 , XM_004944101.3 , XM_004944102.2 , XM_004944102.3
    Accession Version XM_004944101.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product puratrophin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006098.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004944101.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGGTG TGATTCCTTC CCAGGGCGTC TGTGCCGCAA AAGAAATCCG CATGCAGGAG
    ATGGTCTCCA TGGGTGTGGG CAACAAGCCA TTCCTGGATA TCAAACCCAG TGAGGCTGCT
    ATCAATGACC GTGCTATTGA CTACATCATG AAAGGCAGAG CTGCCAGGAC CAGAGCATCA
    ATTGCGGTGT CTCTCTTTGA CCATTCCAAC CCCTACAAGA GGACGCAGGC ACAGCTTTCT
    GCTGGCAGCA CCCCTGCTGC ATATGGCCCA TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC
    CTGCACATGT ACCTGGACCA GGCACTGCTG CCTGGTGTCC TGGCACCCCG GCGCCCCTTT
    GATGTCAGCC CCTGCATTGA GGAAGATGAG CTGGAGCATG AGACGAGCAG CCAGCCATCA
    CTGACCACAG AGAGCTCCGA GTCATCACAC TGCATGTCAG GCAGCGGCTC CAGCGGCTCC
    GACAGTGGCT GTGTCTCCAG CATCCCACAA GAAAGCTTCT GTGAGGACAT GGGTTCACCT
    CCCTTCATGC CCCTGGGCTC ACAACACAGC TCTGCGATGG AGGGCAAACC CCGGTTCACG
    AACAGCCAGT ACATTTCTGC AAAAGCAGGC CAGGCCACCA TAAGTCCCTC GACAATAGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004944158.2
    CDS56..6538
    Translation

    Target ORF information:

    RefSeq Version XM_004944101.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004944101.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGGTG TGATTCCTTC CCAGGGCGTC TGTGCCGCAA AAGAAATCCG CATGCAGGAG
    ATGGTCTCCA TGGGTGTGGG CAACAAGCCA TTCCTGGATA TCAAACCCAG TGAGGCTGCT
    ATCAATGACC GTGCTATTGA CTACATCATG AAAGGCAGAG CTGCCAGGAC CAGAGCATCA
    ATTGCGGTGT CTCTCTTTGA CCATTCCAAC CCCTACAAGA GGACGCAGGC ACAGCTTTCT
    GCTGGCAGCA CCCCTGCTGC ATATGGCCCA TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC
    CTGCACATGT ACCTGGACCA GGCACTGCTG CCTGGTGTCC TGGCACCCCG GCGCCCCTTT
    GATGTCAGCC CCTGCATTGA GGAAGATGAG CTGGAGCATG AGACGAGCAG CCAGCCATCA
    CTGACCACAG AGAGCTCCGA GTCATCACAC TGCATGTCAG GCAGCGGCTC CAGCGGCTCC
    GACAGTGGCT GTGTCTCCAG CATCCCACAA GAAAGCTTCT GTGAGGACAT GGGTTCACCT
    CCCTTCATGC CCCTGGGCTC ACAACACAGC TCTGCGATGG AGGGCAAACC CCGGTTCACG
    AACAGCCAGT ACATTTCTGC AAAAGCAGGC CAGGCCACCA TAAGTCCCTC GACAATAGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40848
    Clone ID Related Accession (Same CDS sequence) XM_004944101.2 , XM_004944101.3 , XM_004944102.2 , XM_004944102.3
    Accession Version XM_004944102.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product puratrophin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763868.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004944102.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGGTG TGATTCCTTC CCAGGGCGTC TGTGCCGCAA AAGAAATCCG CATGCAGGAG
    ATGGTCTCCA TGGGTGTGGG CAACAAGCCA TTCCTGGATA TCAAACCCAG TGAGGCTGCT
    ATCAATGACC GTGCTATTGA CTACATCATG AAAGGCAGAG CTGCCAGGAC CAGAGCATCA
    ATTGCGGTGT CTCTCTTTGA CCATTCCAAC CCCTACAAGA GGACGCAGGC ACAGCTTTCT
    GCTGGCAGCA CCCCTGCTGC ATATGGCCCA TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC
    CTGCACATGT ACCTGGACCA GGCACTGCTG CCTGGTGTCC TGGCACCCCG GCGCCCCTTT
    GATGTCAGCC CCTGCATTGA GGAAGATGAG CTGGAGCATG AGACGAGCAG CCAGCCATCA
    CTGACCACAG AGAGCTCCGA GTCATCACAC TGCATGTCAG GCAGCGGCTC CAGCGGCTCC
    GACAGTGGCT GTGTCTCCAG CATCCCACAA GAAAGCTTCT GTGAGGACAT GGGTTCACCT
    CCCTTCATGC CCCTGGGCTC ACAACACAGC TCTGCGATGG AGGGCAAACC CCGGTTCACG
    AACAGCCAGT ACATTTCTGC AAAAGCAGGC CAGGCCACCA TAAGTCCCTC GACAATAGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004944159.2
    CDS24..6506
    Translation

    Target ORF information:

    RefSeq Version XM_004944102.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004944102.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGGTG TGATTCCTTC CCAGGGCGTC TGTGCCGCAA AAGAAATCCG CATGCAGGAG
    ATGGTCTCCA TGGGTGTGGG CAACAAGCCA TTCCTGGATA TCAAACCCAG TGAGGCTGCT
    ATCAATGACC GTGCTATTGA CTACATCATG AAAGGCAGAG CTGCCAGGAC CAGAGCATCA
    ATTGCGGTGT CTCTCTTTGA CCATTCCAAC CCCTACAAGA GGACGCAGGC ACAGCTTTCT
    GCTGGCAGCA CCCCTGCTGC ATATGGCCCA TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC
    CTGCACATGT ACCTGGACCA GGCACTGCTG CCTGGTGTCC TGGCACCCCG GCGCCCCTTT
    GATGTCAGCC CCTGCATTGA GGAAGATGAG CTGGAGCATG AGACGAGCAG CCAGCCATCA
    CTGACCACAG AGAGCTCCGA GTCATCACAC TGCATGTCAG GCAGCGGCTC CAGCGGCTCC
    GACAGTGGCT GTGTCTCCAG CATCCCACAA GAAAGCTTCT GTGAGGACAT GGGTTCACCT
    CCCTTCATGC CCCTGGGCTC ACAACACAGC TCTGCGATGG AGGGCAAACC CCGGTTCACG
    AACAGCCAGT ACATTTCTGC AAAAGCAGGC CAGGCCACCA TAAGTCCCTC GACAATAGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40848
    Clone ID Related Accession (Same CDS sequence) XM_004944101.2 , XM_004944101.3 , XM_004944102.2 , XM_004944102.3
    Accession Version XM_004944102.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product puratrophin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006098.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004944102.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGGTG TGATTCCTTC CCAGGGCGTC TGTGCCGCAA AAGAAATCCG CATGCAGGAG
    ATGGTCTCCA TGGGTGTGGG CAACAAGCCA TTCCTGGATA TCAAACCCAG TGAGGCTGCT
    ATCAATGACC GTGCTATTGA CTACATCATG AAAGGCAGAG CTGCCAGGAC CAGAGCATCA
    ATTGCGGTGT CTCTCTTTGA CCATTCCAAC CCCTACAAGA GGACGCAGGC ACAGCTTTCT
    GCTGGCAGCA CCCCTGCTGC ATATGGCCCA TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC
    CTGCACATGT ACCTGGACCA GGCACTGCTG CCTGGTGTCC TGGCACCCCG GCGCCCCTTT
    GATGTCAGCC CCTGCATTGA GGAAGATGAG CTGGAGCATG AGACGAGCAG CCAGCCATCA
    CTGACCACAG AGAGCTCCGA GTCATCACAC TGCATGTCAG GCAGCGGCTC CAGCGGCTCC
    GACAGTGGCT GTGTCTCCAG CATCCCACAA GAAAGCTTCT GTGAGGACAT GGGTTCACCT
    CCCTTCATGC CCCTGGGCTC ACAACACAGC TCTGCGATGG AGGGCAAACC CCGGTTCACG
    AACAGCCAGT ACATTTCTGC AAAAGCAGGC CAGGCCACCA TAAGTCCCTC GACAATAGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004944159.2
    CDS56..6538
    Translation

    Target ORF information:

    RefSeq Version XM_004944102.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004944102.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGGTG TGATTCCTTC CCAGGGCGTC TGTGCCGCAA AAGAAATCCG CATGCAGGAG
    ATGGTCTCCA TGGGTGTGGG CAACAAGCCA TTCCTGGATA TCAAACCCAG TGAGGCTGCT
    ATCAATGACC GTGCTATTGA CTACATCATG AAAGGCAGAG CTGCCAGGAC CAGAGCATCA
    ATTGCGGTGT CTCTCTTTGA CCATTCCAAC CCCTACAAGA GGACGCAGGC ACAGCTTTCT
    GCTGGCAGCA CCCCTGCTGC ATATGGCCCA TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC
    CTGCACATGT ACCTGGACCA GGCACTGCTG CCTGGTGTCC TGGCACCCCG GCGCCCCTTT
    GATGTCAGCC CCTGCATTGA GGAAGATGAG CTGGAGCATG AGACGAGCAG CCAGCCATCA
    CTGACCACAG AGAGCTCCGA GTCATCACAC TGCATGTCAG GCAGCGGCTC CAGCGGCTCC
    GACAGTGGCT GTGTCTCCAG CATCCCACAA GAAAGCTTCT GTGAGGACAT GGGTTCACCT
    CCCTTCATGC CCCTGGGCTC ACAACACAGC TCTGCGATGG AGGGCAAACC CCGGTTCACG
    AACAGCCAGT ACATTTCTGC AAAAGCAGGC CAGGCCACCA TAAGTCCCTC GACAATAGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40849
    Clone ID Related Accession (Same CDS sequence) XM_003641900.4 , XM_003641900.3
    Accession Version XM_003641900.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product puratrophin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006098.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_003641900.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGGTG TGATTCCTTC CCAGGGCGTC TGTGCCGCAA AAGAAATCCG CATGCAGGAG
    ATGGTCTCCA TGGGTGTGGG CAACAAGCCA TTCCTGGATA TCAAACCCAG TGAGGCTGCT
    ATCAATGACC GTGCTATTGA CTACATCATG AAAGGCAGAG CTGCCAGGAC CAGAGCATCA
    ATTGCGGTGT CTCTCTTTGA CCATTCCAAC CCCTACAAGA GGACGCAGGC ACAGCTTTCT
    GCTGGCAGCA CCCCTGCTGC ATATGGCCCA TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC
    CTGCACATGT ACCTGGACCA GGCACTGCTG CCTGGTGTCC TGGCACCCCG GCGCCCCTTT
    GATGTCAGCC CCTGCATTGA GGAAGATGAG CTGGAGCATG AGACGAGCAG CCAGCCATCA
    CTGACCACAG AGAGCTCCGA GTCATCACAC TGCATGTCAG GCAGCGGCTC CAGCGGCTCC
    GACAGTGGCT GTGTCTCCAG CATCCCACAA GAAAGCTTCT GTGAGGACAT GGGTTCACCT
    CCCTTCATGC CCCTGGGCTC ACAACACAGC TCTGCGATGG AGGGCAAACC CCGGTTCACG
    AACAGCCAGT ACATTTCTGC AGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003641948.3
    CDS56..6502
    Translation

    Target ORF information:

    RefSeq Version XM_003641900.4
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003641900.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGGTG TGATTCCTTC CCAGGGCGTC TGTGCCGCAA AAGAAATCCG CATGCAGGAG
    ATGGTCTCCA TGGGTGTGGG CAACAAGCCA TTCCTGGATA TCAAACCCAG TGAGGCTGCT
    ATCAATGACC GTGCTATTGA CTACATCATG AAAGGCAGAG CTGCCAGGAC CAGAGCATCA
    ATTGCGGTGT CTCTCTTTGA CCATTCCAAC CCCTACAAGA GGACGCAGGC ACAGCTTTCT
    GCTGGCAGCA CCCCTGCTGC ATATGGCCCA TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC
    CTGCACATGT ACCTGGACCA GGCACTGCTG CCTGGTGTCC TGGCACCCCG GCGCCCCTTT
    GATGTCAGCC CCTGCATTGA GGAAGATGAG CTGGAGCATG AGACGAGCAG CCAGCCATCA
    CTGACCACAG AGAGCTCCGA GTCATCACAC TGCATGTCAG GCAGCGGCTC CAGCGGCTCC
    GACAGTGGCT GTGTCTCCAG CATCCCACAA GAAAGCTTCT GTGAGGACAT GGGTTCACCT
    CCCTTCATGC CCCTGGGCTC ACAACACAGC TCTGCGATGG AGGGCAAACC CCGGTTCACG
    AACAGCCAGT ACATTTCTGC AGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40849
    Clone ID Related Accession (Same CDS sequence) XM_003641900.4 , XM_003641900.3
    Accession Version XM_003641900.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product puratrophin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763868.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_003641900.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGGTG TGATTCCTTC CCAGGGCGTC TGTGCCGCAA AAGAAATCCG CATGCAGGAG
    ATGGTCTCCA TGGGTGTGGG CAACAAGCCA TTCCTGGATA TCAAACCCAG TGAGGCTGCT
    ATCAATGACC GTGCTATTGA CTACATCATG AAAGGCAGAG CTGCCAGGAC CAGAGCATCA
    ATTGCGGTGT CTCTCTTTGA CCATTCCAAC CCCTACAAGA GGACGCAGGC ACAGCTTTCT
    GCTGGCAGCA CCCCTGCTGC ATATGGCCCA TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC
    CTGCACATGT ACCTGGACCA GGCACTGCTG CCTGGTGTCC TGGCACCCCG GCGCCCCTTT
    GATGTCAGCC CCTGCATTGA GGAAGATGAG CTGGAGCATG AGACGAGCAG CCAGCCATCA
    CTGACCACAG AGAGCTCCGA GTCATCACAC TGCATGTCAG GCAGCGGCTC CAGCGGCTCC
    GACAGTGGCT GTGTCTCCAG CATCCCACAA GAAAGCTTCT GTGAGGACAT GGGTTCACCT
    CCCTTCATGC CCCTGGGCTC ACAACACAGC TCTGCGATGG AGGGCAAACC CCGGTTCACG
    AACAGCCAGT ACATTTCTGC AGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003641948.3
    CDS23..6469
    Translation

    Target ORF information:

    RefSeq Version XM_003641900.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003641900.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGAAGAATC GCAGCAGCGA GCGGCTTCCC CCGCTACCAA CCAGCTCCAG CAGCACTCTG 
    GGTTGCAGCG CGCTGAGGAC CGCGGGCACA AGCGTTCCCC CACATCTCCC CTTGGCCTGC
    AAAGGCTCAC AGCAGCGGTG CCCGGGCGGC AGGGCTGTGC TCCATCCACC CCATGACACT
    CTCGCTCACT CGCACGCACT CACGTGCTGC GGTAATGGTT TAATAGCAGC CCCACCCAGC
    TCAGCAAGAC GGACTTGCTG CGTAAGCAAA AGCTGGGGAA CCTCGCCGCT GTGCGCAGCG
    CTCCGTGAGA GCTTCGCTCG CCTTTTGGGA GAGGACTCGG AGTCGCTGGA CGGCTGCATT
    CAGAGCACGT TATCAGCTCT CTACCCTCCG TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC
    CAAGTTTTTG ATGTGGTGGA GAAGACGTAC CGTGGGGATG GACTGCGCTA CCTCATAGAC
    TTCCTGATTC CAGCCAAGCA CATCCTGCAG TGCATTCAGC AGGATGCCTG TGTTCAGTAC
    TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG CCCCTGTGCA TTCACGAGAA GATCGTAGTC
    CAGCTGGCTT CCATTGACTG GCGAATCTTG AAGCCTGGTG ACTTCTACCT GCAGGTGGTT
    CCCTATCTGA AGAAGTCTCC ACGGATTGTA TTGAAGTGTT TAGCAAAAGA CAAGCATAAT
    GTAGAAGAAG TTGTGATTCC AGAAGTTTCC TATACCTCTA TTTTTACTCT GGAATGGTTG
    AGTACTATCA ATGGAGAGCG GATGGGTATA GCACTGGAAA ATTGTTTACT AACCACTGAT
    GATAAAATAT TTCGTGTTCC CTGGGATAAC GTGGTTAATC CGGAATTTAT AAATAAACCA
    AAAATAACTG AAAATAATAT TATCACACCA GAGACAACAG GGAAACCTTC ACTTTTGTGT
    CTCCCCATGG ACCATGCTGA GCGCAGCTTG CCAGACGTAG AATCTCAGTA CCGACAGGAA
    CAAAGTCCAA ATTGCAACAA CCTGGTTGTT AGCAGCCCAG CTCCGCAGTT AGGTGAAACT
    CTTGTCAATG GTGTGTGTTC CAGTCCTGTT CCTAAAGAGG ATTTGCCAAG TGACCTTGAA
    GGGGAGTACG TTGAGCTTGC CGAAGTTTCG CTGCCCCGGT TTGGACCACA GACAGGCTCG
    TTAACCCAGT CACTAGCTTT AAATTATCTG ACTCAGCCTA GAACAAGAGT GAATGCATCT
    AAAGGCAAGC ACAGATTTAT TGTCATTCAA GATAGCACCT ACTCCAAGAA CTTAGTTCAC
    TCAGCACTTA TGCAAAAGGA GCACTGTCAG ATGGACACAC TGAGCACTTC TCCAGAAGTT
    CTCAAAAATG TAATTCCTTT GGAAAGCAAC AAAACGACTG CATGTGAGGA GCTGTCTCCA
    GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG AGCATGGGAA ACAGTTTTCC TGCAGCTGCA
    GCTCCTTCCT GCAGTGCAGA AGATTCCTGT GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC
    TCTGTTGATT GGCAGTATGC GCCGTGCAGC AACGGTGACG TGTGTGCTGG AGATGGTGTC
    AGCTGCAAAG CACAAATGAC TGGTTCTACT GGAAACATGG AATTAGAAAG CGATGGTTCT
    AGCATAAATC CCGGTGTTGA AATACTGGAG CAGAGCCCAG GAGCGAATCT AGATGCTGCT
    GCTGAAGAGG AGGTTATGGA AGAACACAGA GAACCACTGG CAGAGCATCA ACATGAGGTG
    ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC CTGGGGCCTT TGGGACCGTG CTGCACGCTG
    AAGGAAGGTC CCCATGCTTC TTGCCAGAGT GCTGGTGAAA GTACTATACC AGTCCACGCC
    ACTGCATTGC CTGCCACGCC TGAGAGTTTG GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA
    GGGAACGTGA CAGATGCAGC AGCAGTCCGA GCTTGTGCTG ATCAGGAAAC AAATTATAGT
    TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG
    AGAAGAGAGA GCCCTGAGGA TGAAGAGATT GAACAAGTGC AGGCTGTGAA GGAGCATCGG
    AGTAGCTGTA GTTGCTGTTC TACCAACGCT CCTGCGGATG GAGTGCTTTC AGATGGTGAA
    GAACAAGAGG TCCCTGCTTG TCAGCATAAG AGAGCTGAAG ATGCCATGCT GCCCCAAACT
    GCACTGCCAG CAGAGAGTGG GCAGGTGGCA GAACAGTCAG GTACCAGCGG ATCCTTGCTG
    GGTCTTGATG CTCAAGCAGA AGACTCGAGT CAGTTTGGAA GCCACAGATC TGGAGAGAGG
    TGTGGGGATC CACGAGGATG GACAGAAAAC CAAGGCAATG GAAGTGAAAT GAACTTTGTG
    CTGAGCCCAG AGTGTGTTTC AGCACAGGAC TCGCAGGGAG GGGAGGAGAA CCAAGACAGT
    TTTTCTGATG TGGACAGGAC AAAGGCTGTA GCCTTAAAAA CACTGGAATC CATACAAGAG
    GAGCTGGAGG TTGGACCACT GTCCTCAGGA CCTGCTGAGG GGAACATAGA GCCCACTGCT
    TTGCATTCCC ACCCAGAGGC AGTTCCAGAA GCGTCATCTC CTAAAGGCAT GGTATCGGAC
    GGGGCAGAGG TGGTAAAAGA AGTATCTGGT GCAGATGTAT TGAAGCCAGC AGGTAAAGCC
    TGTGCCCTGG AGGAAACCTC AACAGCTGTT AGTCCCCCCA GATCCCCAGC TCCTGGGGCT
    GCTTGGGGCG GCCGGTCTGC ACCTTCCATA GTCAAGGATG TGAACCCTGA AGTGCTCAGG
    AGTGGAGTTG CCTGCCTGCC TGGTACCAGA GATAAATCGG GACGTGCAGT GGCGATAATA
    ACAACAAGGA ACACAGCCTG GTTAAACCCT CACTGCAACA CCACTGAGCT GGTTCGCCTC
    CTCCTGTACC TGCACAGCAT CCCAAGACCC GAGTGTCAGG CTTTGGGTCT GACTGTTCTC
    GTGGATGCTC GTCGCTGTTC GCCGGTTCCT GCTCTCTTCA AGGCATTCAG CATTTTGCAG
    GACATGGATC CTCATTGTAT TCATGGAGTA CTGCTTCTGG TTGAAAGAGA TCTGACTTTC
    CGGATGGAGA AGCCGACAGC AGGACAGTTT GAAATTCTCA CCTCCATGAA ATCCCTCCAT
    AAGCACATAG ACAGCTCACA GCTGCCTCTG GAACTGGACG GGACCTTCCC ATACTGCCAC
    CGGGACTGGT TAAGTTTCCG CATGAAACTG GAACACTTGC TACAGGGATG CCAAGGGGCT
    TGTGCCTTCC TCCAGGGAGC CATTCATAGA GTGGAATCTG GAAAATTACC AGAAAGAGCT
    GAGGAGGCTG CTGTGCTGCT GAGGAACTAC AGGCAGCTGA TGAAGAATGT TCTTGAGGAT
    GCACGGCTGG TCAGGCTTCA GCTGGAGGGT GGAGCACTGC TGGCCAGGCT GAGGAAGGAG
    GAGTCTTGTG TCACCCTCAC CCAGGACTAC AGGGACGCGC TTGATGCTGC TGGTGTGCTT
    TATAATCGAG TCGATGAGGG CGTTCACCGG CTGGTGATGG CGTCAAATAA ACGCATCCAG
    GAGCTGGAGC TGGTGATGGA GTTTGAGAAA GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT
    TGGATTGAGA ATGTTGGCAG AAGAAAGCTA AAGGAGATGA TCAACCTGGA CGATTCTTTG
    GAGATGCTGC TTCAAGCACA GAAGCAGTTC AGGGAGTTTG ATCTTGTTGC CAGTGAATAT
    TGCAGAAGGG GACAGGAAGC ACTTAAGAAG ATGGATCGTT GGGAAGACTT TTCCTCTGTG
    GATGTACATT CTTACAGAGT GAAGCTGCAG ACCTACAGAG ATCAACTGGA AGAATTCTGC
    ACTCAACTGG ATGAAAGCAG ACATCGAGTC TGTGAGACAG TCAGGCTGTA TGAATTCTTT
    GACAAGGCGT ACGAGTGGGC CTTGGAAGGG ATGCGACACC TCGCCTGCAT CAGCATGGAG
    GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG GTCATCAAGT GCCTGGAGAG CTACAAGGAA
    CAGCACCCAG ACATCAGCGA TGCCCGCTTC CAGGAGATGA AGGAGCTGGC CTGCGAACTG
    AAGAGCGACA AGGGACTCAA GCAGTGGAAA TTCGCGTGGT CCAAATGCCA AGAGACCAAG
    CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC TTGAGAACCA GGAGGGCACT GCTGTCGGAG
    CAGCCACAGG TGGGCAGCGA GGCTGGCAGC GTGTCCCACC GCAGGCACTC TGAGGGGGCT
    GCCCCCGGCC CACAGTGGGA CAGAACTCCT GGCACATCCC ACAGCCGGCC CAGTCGCTCC
    ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA CCCTCCCTGA GCTGCCCTGG TGATTTCGGT
    GCTGGGGAAG AATTTGCCTG CCAAGCTGCA CTCGGGGCCG GGCCTCACGT TGGCAGAGTT
    GCTTTTGGAA GCGGGGCCTG CAAGTCACCA AAACTGCCCG AGGAGAAGTC AAACCTGGAG
    AGCATCTTAG AAACGTTGGA GATGAAGCCA TCCTGTGCCT CCACACCTGC CTCTGGGAAG
    CTGCCTCCCA AGAGGATGCT CCGTAAGGCC CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC
    CTGTGGTCGG GCTGCCAGAG GACGCTGAGC GAGCCAGCCA GGTACGGCAA CACTGGTGTC
    TTCATCAAGG GACTGGAGGT GAGCAGCACG GAGCTGGTGG ACAGGACATT TGCACTGCGG
    CAGCTGGAGG GACAGCGTGG CTGTGGCTCT GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG
    CGGCACATCA TCGATGAGAT GGTGACCACG GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC
    ATCATTGAAA GCTACTTCCC TGAGATGGAG CGCCTGGATC TGCCCCAGGA CCTGCGGGGG
    AAGCGCAGCG TCATTTTTGG GAACCTAGAG AAACTCTATG ACTTCCACAG TCAGTACTTC
    CTGCGAGAGC TCGAGAGCTG CTGCAACCAC CCGCTGCGCG TCAGCCACTG CTTCCTGCGG
    CACAAAGACC AGTTTGGGAT GTACGCCTTA TACAGCAAGA ACAAGCCAAA GTCAGACTCA
    CTGCTGGCCA GCCATGGGAA CACCTTCTTC AAGTTCAAGC AGGTGCAGCT GGGGGATAAG
    ATGGACCTGG CTTCCTACCT GCTGAAACCC ATCCAGCGCA TGAGCAAATA CGCCCTGCTC
    CTGAAGGACC TGATCAAGGA GTGCAGTGAG GCCCAGGAGC AGGAGCTGGG CTACCTCCGG
    GCAGCAGAGG AGATGGTGAA GTTCCAGCTA CGGCATGGGA ATGACCTGCT GGCTATGGAT
    GCCATCCGTG ACTGTGACGT GAACCTGAAG GAGCAGGGGC AGCTGGTACG GCAGGACGAG
    TTTGCCATCT GGCTGGGCCG CAGGAAGTGC CAGCGCCACG TCTTCCTCTT TGAGGACCTG
    ATCCTGTTCA GCAAGCCCAA GAGGATCGAG GGTGGCTTTG ATGTGTATAT CTACAAGCGC
    TCCTTCAAGA CTGCAGACAT CGGTCTGACG GAAAACTCTG GCGACAGTGG TCTGCGTTTT
    GAGATCTGGT TCCGAAGGCG GAAGTCCAGC GACACTTACA TCCTCCAGGC CAGCTCAGCT
    GAGACCAAGC AGGCCTGGAC CAGTGACATT GCCAAGATCC TGTGGCAGCA GGCAGCCCGC
    AATAAAGGTG TGATTCCTTC CCAGGGCGTC TGTGCCGCAA AAGAAATCCG CATGCAGGAG
    ATGGTCTCCA TGGGTGTGGG CAACAAGCCA TTCCTGGATA TCAAACCCAG TGAGGCTGCT
    ATCAATGACC GTGCTATTGA CTACATCATG AAAGGCAGAG CTGCCAGGAC CAGAGCATCA
    ATTGCGGTGT CTCTCTTTGA CCATTCCAAC CCCTACAAGA GGACGCAGGC ACAGCTTTCT
    GCTGGCAGCA CCCCTGCTGC ATATGGCCCA TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC
    CTGCACATGT ACCTGGACCA GGCACTGCTG CCTGGTGTCC TGGCACCCCG GCGCCCCTTT
    GATGTCAGCC CCTGCATTGA GGAAGATGAG CTGGAGCATG AGACGAGCAG CCAGCCATCA
    CTGACCACAG AGAGCTCCGA GTCATCACAC TGCATGTCAG GCAGCGGCTC CAGCGGCTCC
    GACAGTGGCT GTGTCTCCAG CATCCCACAA GAAAGCTTCT GTGAGGACAT GGGTTCACCT
    CCCTTCATGC CCCTGGGCTC ACAACACAGC TCTGCGATGG AGGGCAAACC CCGGTTCACG
    AACAGCCAGT ACATTTCTGC AGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40850
    Clone ID Related Accession (Same CDS sequence) XM_015279197.1
    Accession Version XM_015279197.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product puratrophin-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006098.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGGAGAAT GGTTTGCAGG ATCTGCTGGG ATCTGCTGGG TGCAGGCTGC TCTGCAAGTG 
    CTGGATGACT CGGAGTCGCT GGACGGCTGC ATTCAGAGCA CGTTATCAGC TCTCTACCCT
    CCGTTCGAGG CTACGGCCGC CACCGTGCTG TGCCAAGTTT TTGATGTGGT GGAGAAGACG
    TACCGTGGGG ATGGACTGCG CTACCTCATA GACTTCCTGA TTCCAGCCAA GCACATCCTG
    CAGTGCATTC AGCAGGATGC CTGTGTTCAG TACTGTGGCT TGCTGTTTCG CCACGAGGGC
    TGGCCCCTGT GCATTCACGA GAAGATCGTA GTCCAGCTGG CTTCCATTGA CTGGCGAATC
    TTGAAGCCTG GTGACTTCTA CCTGCAGGTG GTTCCCTATC TGAAGAAGTC TCCACGGATT
    GTATTGAAGT GTTTAGCAAA AGACAAGCAT AATGTAGAAG AAGTTGTGAT TCCAGAAGTT
    TCCTATACCT CTATTTTTAC TCTGGAATGG TTGAGTACTA TCAATGGAGA GCGGATGGGT
    ATAGCACTGG AAAATTGTTT ACTAACCACT GATGATAAAA TATTTCGTGT TCCCTGGGAT
    AACGTGGTTA ATCCGGAATT TATAAATAAA CCAAAAATAA CTGAAAATAA TATTATCACA
    CCAGAGACAA CAGGGAAACC TTCACTTTTG TGTCTCCCCA TGGACCATGC TGAGCGCAGC
    TTGCCAGACG TAGAATCTCA GTACCGACAG GAACAAAGTC CAAATTGCAA CAACCTGGTT
    GTTAGCAGCC CAGCTCCGCA GTTAGGTGAA ACTCTTGTCA ATGGTGTGTG TTCCAGTCCT
    GTTCCTAAAG AGGATTTGCC AAGTGACCTT GAAGGGGAGT ACGTTGAGCT TGCCGAAGTT
    TCGCTGCCCC GGTTTGGACC ACAGACAGGC TCGTTAACCC AGTCACTAGC TTTAAATTAT
    CTGACTCAGC CTAGAACAAG AGTGAATGCA TCTAAAGGCA AGCACAGATT TATTGTCATT
    CAAGATAGCA CCTACTCCAA GAACTTAGTT CACTCAGCAC TTATGCAAAA GGAGCACTGT
    CAGATGGACA CACTGAGCAC TTCTCCAGAA GTTCTCAAAA ATGTAATTCC TTTGGAAAGC
    AACAAAACGA CTGCATGTGA GGAGCTGTCT CCAGAAGGTC AGCTGCTGTC AGGTGGTCCT
    GGGAGCATGG GAAACAGTTT TCCTGCAGCT GCAGCTCCTT CCTGCAGTGC AGAAGATTCC
    TGTGCTGCAG AGCAGGAGGC CTGGAGTGAG TGCTCTGTTG ATTGGCAGTA TGCGCCGTGC
    AGCAACGGTG ACGTGTGTGC TGGAGATGGT GTCAGCTGCA AAGCACAAAT GACTGGTTCT
    ACTGGAAACA TGGAATTAGA AAGCGATGGT TCTAGCATAA ATCCCGGTGT TGAAATACTG
    GAGCAGAGCC CAGGAGCGAA TCTAGATGCT GCTGCTGAAG AGGAGGTTAT GGAAGAACAC
    AGAGAACCAC TGGCAGAGCA TCAACATGAG GTGACGCAGG TGGATCCCTC TCTTGGGTCT
    GTCCTGGGGC CTTTGGGACC GTGCTGCACG CTGAAGGAAG GTCCCCATGC TTCTTGCCAG
    AGTGCTGGTG AAAGTACTAT ACCAGTCCAC GCCACTGCAT TGCCTGCCAC GCCTGAGAGT
    TTGGCTGTGG GTGTGGAGAG AAGCTCTCCT TCAGGGAACG TGACAGATGC AGCAGCAGTC
    CGAGCTTGTG CTGATCAGGA AACAAATTAT AGTTGTCCAG TAAATCCTCC AGAGCAGTTG
    AACGGAGGAG AAGTAGGTGA AATAGAGCTG GGGAGAAGAG AGAGCCCTGA GGATGAAGAG
    ATTGAACAAG TGCAGGCTGT GAAGGAGCAT CGGAGTAGCT GTAGTTGCTG TTCTACCAAC
    GCTCCTGCGG ATGGAGTGCT TTCAGATGGT GAAGAACAAG AGGTCCCTGC TTGTCAGCAT
    AAGAGAGCTG AAGATGCCAT GCTGCCCCAA ACTGCACTGC CAGCAGAGAG TGGGCAGGTG
    GCAGAACAGT CAGGTACCAG CGGATCCTTG CTGGGTCTTG ATGCTCAAGC AGAAGACTCG
    AGTCAGTTTG GAAGCCACAG ATCTGGAGAG AGGTGTGGGG ATCCACGAGG ATGGACAGAA
    AACCAAGGCA ATGGAAGTGA AATGAACTTT GTGCTGAGCC CAGAGTGTGT TTCAGCACAG
    GACTCGCAGG GAGGGGAGGA GAACCAAGAC AGTTTTTCTG ATGTGGACAG GACAAAGGCT
    GTAGCCTTAA AAACACTGGA ATCCATACAA GAGGAGCTGG AGGTTGGACC ACTGTCCTCA
    GGACCTGCTG AGGGGAACAT AGAGCCCACT GCTTTGCATT CCCACCCAGA GGCAGTTCCA
    GAAGCGTCAT CTCCTAAAGG CATGGTATCG GACGGGGCAG AGGTGGTAAA AGAAGTATCT
    GGTGCAGATG TATTGAAGCC AGCAGGTAAA GCCTGTGCCC TGGAGGAAAC CTCAACAGCT
    GTTAGTCCCC CCAGATCCCC AGCTCCTGGG GCTGCTTGGG GCGGCCGGTC TGCACCTTCC
    ATAGTCAAGG ATGTGAACCC TGAAGTGCTC AGGAGTGGAG TTGCCTGCCT GCCTGGTACC
    AGAGATAAAT CGGGACGTGC AGTGGCGATA ATAACAACAA GGAACACAGC CTGGTTAAAC
    CCTCACTGCA ACACCACTGA GCTGGTTCGC CTCCTCCTGT ACCTGCACAG CATCCCAAGA
    CCCGAGTGTC AGGCTTTGGG TCTGACTGTT CTCGTGGATG CTCGTCGCTG TTCGCCGGTT
    CCTGCTCTCT TCAAGGCATT CAGCATTTTG CAGGACATGG ATCCTCATTG TATTCATGGA
    GTACTGCTTC TGGTTGAAAG AGATCTGACT TTCCGGATGG AGAAGCCGAC AGCAGGACAG
    TTTGAAATTC TCACCTCCAT GAAATCCCTC CATAAGCACA TAGACAGCTC ACAGCTGCCT
    CTGGAACTGG ACGGGACCTT CCCATACTGC CACCGGGACT GGTTAAGTTT CCGCATGAAA
    CTGGAACACT TGCTACAGGG ATGCCAAGGG GCTTGTGCCT TCCTCCAGGG AGCCATTCAT
    AGAGTGGAAT CTGGAAAATT ACCAGAAAGA GCTGAGGAGG CTGCTGTGCT GCTGAGGAAC
    TACAGGCAGC TGATGAAGAA TGTTCTTGAG GATGCACGGC TGGTCAGGCT TCAGCTGGAG
    GGTGGAGCAC TGCTGGCCAG GCTGAGGAAG GAGGAGTCTT GTGTCACCCT CACCCAGGAC
    TACAGGGACG CGCTTGATGC TGCTGGTGTG CTTTATAATC GAGTCGATGA GGGCGTTCAC
    CGGCTGGTGA TGGCGTCAAA TAAACGCATC CAGGAGCTGG AGCTGGTGAT GGAGTTTGAG
    AAAGTGGAAG AATGTTTTAA GGAGGTCAGC AGTTGGATTG AGAATGTTGG CAGAAGAAAG
    CTAAAGGAGA TGATCAACCT GGACGATTCT TTGGAGATGC TGCTTCAAGC ACAGAAGCAG
    TTCAGGGAGT TTGATCTTGT TGCCAGTGAA TATTGCAGAA GGGGACAGGA AGCACTTAAG
    AAGATGGATC GTTGGGAAGA CTTTTCCTCT GTGGATGTAC ATTCTTACAG AGTGAAGCTG
    CAGACCTACA GAGATCAACT GGAAGAATTC TGCACTCAAC TGGATGAAAG CAGACATCGA
    GTCTGTGAGA CAGTCAGGCT GTATGAATTC TTTGACAAGG CGTACGAGTG GGCCTTGGAA
    GGGATGCGAC ACCTCGCCTG CATCAGCATG GAGGAGTGCA GCTCCGCCGA GCACTGCGCC
    GGGGTCATCA AGTGCCTGGA GAGCTACAAG GAACAGCACC CAGACATCAG CGATGCCCGC
    TTCCAGGAGA TGAAGGAGCT GGCCTGCGAA CTGAAGAGCG ACAAGGGACT CAAGCAGTGG
    AAATTCGCGT GGTCCAAATG CCAAGAGACC AAGCTGGTTT TTGAAAAGAA GCTGGAAGCA
    GCCTTGAGAA CCAGGAGGGC ACTGCTGTCG GAGCAGCCAC AGGTGGGCAG CGAGGCTGGC
    AGCGTGTCCC ACCGCAGGCA CTCTGAGGGG GCTGCCCCCG GCCCACAGTG GGACAGAACT
    CCTGGCACAT CCCACAGCCG GCCCAGTCGC TCCACGGGGT GGACTAAAGA GAAAGCTCCC
    TCACCCTCCC TGAGCTGCCC TGGTGATTTC GGTGCTGGGG AAGAATTTGC CTGCCAAGCT
    GCACTCGGGG CCGGGCCTCA CGTTGGCAGA GTTGCTTTTG GAAGCGGGGC CTGCAAGTCA
    CCAAAACTGC CCGAGGAGAA GTCAAACCTG GAGAGCATCT TAGAAACGTT GGAGATGAAG
    CCATCCTGTG CCTCCACACC TGCCTCTGGG AAGCTGCCTC CCAAGAGGAT GCTCCGTAAG
    GCCCAAAGCT TTGACCTGCC CTGCGGTGAG AGCCTGTGGT CGGGCTGCCA GAGGACGCTG
    AGCGAGCCAG CCAGGTACGG CAACACTGGT GTCTTCATCA AGGGACTGGA GGTGAGCAGC
    ACGGAGCTGG TGGACAGGAC ATTTGCACTG CGGCAGCTGG AGGGACAGCG TGGCTGTGGC
    TCTGCAGAGG CCAGGAGCTG GGGCAGCAAG CTGCGGCACA TCATCGATGA GATGGTGACC
    ACGGAGCGGG AGTACGTCAG GTCGCTGTGC TACATCATTG AAAGCTACTT CCCTGAGATG
    GAGCGCCTGG ATCTGCCCCA GGACCTGCGG GGGAAGCGCA GCGTCATTTT TGGGAACCTA
    GAGAAACTCT ATGACTTCCA CAGTCAGTAC TTCCTGCGAG AGCTCGAGAG CTGCTGCAAC
    CACCCGCTGC GCGTCAGCCA CTGCTTCCTG CGGCACAAAG ACCAGTTTGG GATGTACGCC
    TTATACAGCA AGAACAAGCC AAAGTCAGAC TCACTGCTGG CCAGCCATGG GAACACCTTC
    TTCAAGTTCA AGCAGGTGCA GCTGGGGGAT AAGATGGACC TGGCTTCCTA CCTGCTGAAA
    CCCATCCAGC GCATGAGCAA ATACGCCCTG CTCCTGAAGG ACCTGATCAA GGAGTGCAGT
    GAGGCCCAGG AGCAGGAGCT GGGCTACCTC CGGGCAGCAG AGGAGATGGT GAAGTTCCAG
    CTACGGCATG GGAATGACCT GCTGGCTATG GATGCCATCC GTGACTGTGA CGTGAACCTG
    AAGGAGCAGG GGCAGCTGGT ACGGCAGGAC GAGTTTGCCA TCTGGCTGGG CCGCAGGAAG
    TGCCAGCGCC ACGTCTTCCT CTTTGAGGAC CTGATCCTGT TCAGCAAGCC CAAGAGGATC
    GAGGGTGGCT TTGATGTGTA TATCTACAAG CGCTCCTTCA AGACTGCAGA CATCGGTCTG
    ACGGAAAACT CTGGCGACAG TGGTCTGCGT TTTGAGATCT GGTTCCGAAG GCGGAAGTCC
    AGCGACACTT ACATCCTCCA GGCCAGCTCA GCTGAGACCA AGCAGGCCTG GACCAGTGAC
    ATTGCCAAGA TCCTGTGGCA GCAGGCAGCC CGCAATAAAG GTGTGATTCC TTCCCAGGGC
    GTCTGTGCCG CAAAAGAAAT CCGCATGCAG GAGATGGTCT CCATGGGTGT GGGCAACAAG
    CCATTCCTGG ATATCAAACC CAGTGAGGCT GCTATCAATG ACCGTGCTAT TGACTACATC
    ATGAAAGGCA GAGCTGCCAG GACCAGAGCA TCAATTGCGG TGTCTCTCTT TGACCATTCC
    AACCCCTACA AGAGGACGCA GGCACAGCTT TCTGCTGGCA GCACCCCTGC TGCATATGGC
    CCATCCTCCT CCTCGCTGCT GGGGCCCCTC AACCTGCACA TGTACCTGGA CCAGGCACTG
    CTGCCTGGTG TCCTGGCACC CCGGCGCCCC TTTGATGTCA GCCCCTGCAT TGAGGAAGAT
    GAGCTGGAGC ATGAGACGAG CAGCCAGCCA TCACTGACCA CAGAGAGCTC CGAGTCATCA
    CACTGCATGT CAGGCAGCGG CTCCAGCGGC TCCGACAGTG GCTGTGTCTC CAGCATCCCA
    CAAGAAAGCT TCTGTGAGGA CATGGGTTCA CCTCCCTTCA TGCCCCTGGG CTCACAACAC
    AGCTCTGCGA TGGAGGGCAA ACCCCGGTTC ACGAACAGCC AGTACATTTC TGCAGTAAGT
    GCAAGCAGCC AAAGCACTCT CTTCGCTCTG CCCGTTCCCA CCACTGCCTC CCCAGTTCAA
    GGCACTGCCT CTCACGTGGG GACTGAAGAT GCTGCTTTTG GGCTGGGAAT TGCTGTGGGG
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134683.1
    CDS16..6321
    Translation

    Target ORF information:

    RefSeq Version XM_015279197.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279197.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGGAGAAT GGTTTGCAGG ATCTGCTGGG ATCTGCTGGG TGCAGGCTGC TCTGCAAGTG 
    CTGGATGACT CGGAGTCGCT GGACGGCTGC ATTCAGAGCA CGTTATCAGC TCTCTACCCT
    CCGTTCGAGG CTACGGCCGC CACCGTGCTG TGCCAAGTTT TTGATGTGGT GGAGAAGACG
    TACCGTGGGG ATGGACTGCG CTACCTCATA GACTTCCTGA TTCCAGCCAA GCACATCCTG
    CAGTGCATTC AGCAGGATGC CTGTGTTCAG TACTGTGGCT TGCTGTTTCG CCACGAGGGC
    TGGCCCCTGT GCATTCACGA GAAGATCGTA GTCCAGCTGG CTTCCATTGA CTGGCGAATC
    TTGAAGCCTG GTGACTTCTA CCTGCAGGTG GTTCCCTATC TGAAGAAGTC TCCACGGATT
    GTATTGAAGT GTTTAGCAAA AGACAAGCAT AATGTAGAAG AAGTTGTGAT TCCAGAAGTT
    TCCTATACCT CTATTTTTAC TCTGGAATGG TTGAGTACTA TCAATGGAGA GCGGATGGGT
    ATAGCACTGG AAAATTGTTT ACTAACCACT GATGATAAAA TATTTCGTGT TCCCTGGGAT
    AACGTGGTTA ATCCGGAATT TATAAATAAA CCAAAAATAA CTGAAAATAA TATTATCACA
    CCAGAGACAA CAGGGAAACC TTCACTTTTG TGTCTCCCCA TGGACCATGC TGAGCGCAGC
    TTGCCAGACG TAGAATCTCA GTACCGACAG GAACAAAGTC CAAATTGCAA CAACCTGGTT
    GTTAGCAGCC CAGCTCCGCA GTTAGGTGAA ACTCTTGTCA ATGGTGTGTG TTCCAGTCCT
    GTTCCTAAAG AGGATTTGCC AAGTGACCTT GAAGGGGAGT ACGTTGAGCT TGCCGAAGTT
    TCGCTGCCCC GGTTTGGACC ACAGACAGGC TCGTTAACCC AGTCACTAGC TTTAAATTAT
    CTGACTCAGC CTAGAACAAG AGTGAATGCA TCTAAAGGCA AGCACAGATT TATTGTCATT
    CAAGATAGCA CCTACTCCAA GAACTTAGTT CACTCAGCAC TTATGCAAAA GGAGCACTGT
    CAGATGGACA CACTGAGCAC TTCTCCAGAA GTTCTCAAAA ATGTAATTCC TTTGGAAAGC
    AACAAAACGA CTGCATGTGA GGAGCTGTCT CCAGAAGGTC AGCTGCTGTC AGGTGGTCCT
    GGGAGCATGG GAAACAGTTT TCCTGCAGCT GCAGCTCCTT CCTGCAGTGC AGAAGATTCC
    TGTGCTGCAG AGCAGGAGGC CTGGAGTGAG TGCTCTGTTG ATTGGCAGTA TGCGCCGTGC
    AGCAACGGTG ACGTGTGTGC TGGAGATGGT GTCAGCTGCA AAGCACAAAT GACTGGTTCT
    ACTGGAAACA TGGAATTAGA AAGCGATGGT TCTAGCATAA ATCCCGGTGT TGAAATACTG
    GAGCAGAGCC CAGGAGCGAA TCTAGATGCT GCTGCTGAAG AGGAGGTTAT GGAAGAACAC
    AGAGAACCAC TGGCAGAGCA TCAACATGAG GTGACGCAGG TGGATCCCTC TCTTGGGTCT
    GTCCTGGGGC CTTTGGGACC GTGCTGCACG CTGAAGGAAG GTCCCCATGC TTCTTGCCAG
    AGTGCTGGTG AAAGTACTAT ACCAGTCCAC GCCACTGCAT TGCCTGCCAC GCCTGAGAGT
    TTGGCTGTGG GTGTGGAGAG AAGCTCTCCT TCAGGGAACG TGACAGATGC AGCAGCAGTC
    CGAGCTTGTG CTGATCAGGA AACAAATTAT AGTTGTCCAG TAAATCCTCC AGAGCAGTTG
    AACGGAGGAG AAGTAGGTGA AATAGAGCTG GGGAGAAGAG AGAGCCCTGA GGATGAAGAG
    ATTGAACAAG TGCAGGCTGT GAAGGAGCAT CGGAGTAGCT GTAGTTGCTG TTCTACCAAC
    GCTCCTGCGG ATGGAGTGCT TTCAGATGGT GAAGAACAAG AGGTCCCTGC TTGTCAGCAT
    AAGAGAGCTG AAGATGCCAT GCTGCCCCAA ACTGCACTGC CAGCAGAGAG TGGGCAGGTG
    GCAGAACAGT CAGGTACCAG CGGATCCTTG CTGGGTCTTG ATGCTCAAGC AGAAGACTCG
    AGTCAGTTTG GAAGCCACAG ATCTGGAGAG AGGTGTGGGG ATCCACGAGG ATGGACAGAA
    AACCAAGGCA ATGGAAGTGA AATGAACTTT GTGCTGAGCC CAGAGTGTGT TTCAGCACAG
    GACTCGCAGG GAGGGGAGGA GAACCAAGAC AGTTTTTCTG ATGTGGACAG GACAAAGGCT
    GTAGCCTTAA AAACACTGGA ATCCATACAA GAGGAGCTGG AGGTTGGACC ACTGTCCTCA
    GGACCTGCTG AGGGGAACAT AGAGCCCACT GCTTTGCATT CCCACCCAGA GGCAGTTCCA
    GAAGCGTCAT CTCCTAAAGG CATGGTATCG GACGGGGCAG AGGTGGTAAA AGAAGTATCT
    GGTGCAGATG TATTGAAGCC AGCAGGTAAA GCCTGTGCCC TGGAGGAAAC CTCAACAGCT
    GTTAGTCCCC CCAGATCCCC AGCTCCTGGG GCTGCTTGGG GCGGCCGGTC TGCACCTTCC
    ATAGTCAAGG ATGTGAACCC TGAAGTGCTC AGGAGTGGAG TTGCCTGCCT GCCTGGTACC
    AGAGATAAAT CGGGACGTGC AGTGGCGATA ATAACAACAA GGAACACAGC CTGGTTAAAC
    CCTCACTGCA ACACCACTGA GCTGGTTCGC CTCCTCCTGT ACCTGCACAG CATCCCAAGA
    CCCGAGTGTC AGGCTTTGGG TCTGACTGTT CTCGTGGATG CTCGTCGCTG TTCGCCGGTT
    CCTGCTCTCT TCAAGGCATT CAGCATTTTG CAGGACATGG ATCCTCATTG TATTCATGGA
    GTACTGCTTC TGGTTGAAAG AGATCTGACT TTCCGGATGG AGAAGCCGAC AGCAGGACAG
    TTTGAAATTC TCACCTCCAT GAAATCCCTC CATAAGCACA TAGACAGCTC ACAGCTGCCT
    CTGGAACTGG ACGGGACCTT CCCATACTGC CACCGGGACT GGTTAAGTTT CCGCATGAAA
    CTGGAACACT TGCTACAGGG ATGCCAAGGG GCTTGTGCCT TCCTCCAGGG AGCCATTCAT
    AGAGTGGAAT CTGGAAAATT ACCAGAAAGA GCTGAGGAGG CTGCTGTGCT GCTGAGGAAC
    TACAGGCAGC TGATGAAGAA TGTTCTTGAG GATGCACGGC TGGTCAGGCT TCAGCTGGAG
    GGTGGAGCAC TGCTGGCCAG GCTGAGGAAG GAGGAGTCTT GTGTCACCCT CACCCAGGAC
    TACAGGGACG CGCTTGATGC TGCTGGTGTG CTTTATAATC GAGTCGATGA GGGCGTTCAC
    CGGCTGGTGA TGGCGTCAAA TAAACGCATC CAGGAGCTGG AGCTGGTGAT GGAGTTTGAG
    AAAGTGGAAG AATGTTTTAA GGAGGTCAGC AGTTGGATTG AGAATGTTGG CAGAAGAAAG
    CTAAAGGAGA TGATCAACCT GGACGATTCT TTGGAGATGC TGCTTCAAGC ACAGAAGCAG
    TTCAGGGAGT TTGATCTTGT TGCCAGTGAA TATTGCAGAA GGGGACAGGA AGCACTTAAG
    AAGATGGATC GTTGGGAAGA CTTTTCCTCT GTGGATGTAC ATTCTTACAG AGTGAAGCTG
    CAGACCTACA GAGATCAACT GGAAGAATTC TGCACTCAAC TGGATGAAAG CAGACATCGA
    GTCTGTGAGA CAGTCAGGCT GTATGAATTC TTTGACAAGG CGTACGAGTG GGCCTTGGAA
    GGGATGCGAC ACCTCGCCTG CATCAGCATG GAGGAGTGCA GCTCCGCCGA GCACTGCGCC
    GGGGTCATCA AGTGCCTGGA GAGCTACAAG GAACAGCACC CAGACATCAG CGATGCCCGC
    TTCCAGGAGA TGAAGGAGCT GGCCTGCGAA CTGAAGAGCG ACAAGGGACT CAAGCAGTGG
    AAATTCGCGT GGTCCAAATG CCAAGAGACC AAGCTGGTTT TTGAAAAGAA GCTGGAAGCA
    GCCTTGAGAA CCAGGAGGGC ACTGCTGTCG GAGCAGCCAC AGGTGGGCAG CGAGGCTGGC
    AGCGTGTCCC ACCGCAGGCA CTCTGAGGGG GCTGCCCCCG GCCCACAGTG GGACAGAACT
    CCTGGCACAT CCCACAGCCG GCCCAGTCGC TCCACGGGGT GGACTAAAGA GAAAGCTCCC
    TCACCCTCCC TGAGCTGCCC TGGTGATTTC GGTGCTGGGG AAGAATTTGC CTGCCAAGCT
    GCACTCGGGG CCGGGCCTCA CGTTGGCAGA GTTGCTTTTG GAAGCGGGGC CTGCAAGTCA
    CCAAAACTGC CCGAGGAGAA GTCAAACCTG GAGAGCATCT TAGAAACGTT GGAGATGAAG
    CCATCCTGTG CCTCCACACC TGCCTCTGGG AAGCTGCCTC CCAAGAGGAT GCTCCGTAAG
    GCCCAAAGCT TTGACCTGCC CTGCGGTGAG AGCCTGTGGT CGGGCTGCCA GAGGACGCTG
    AGCGAGCCAG CCAGGTACGG CAACACTGGT GTCTTCATCA AGGGACTGGA GGTGAGCAGC
    ACGGAGCTGG TGGACAGGAC ATTTGCACTG CGGCAGCTGG AGGGACAGCG TGGCTGTGGC
    TCTGCAGAGG CCAGGAGCTG GGGCAGCAAG CTGCGGCACA TCATCGATGA GATGGTGACC
    ACGGAGCGGG AGTACGTCAG GTCGCTGTGC TACATCATTG AAAGCTACTT CCCTGAGATG
    GAGCGCCTGG ATCTGCCCCA GGACCTGCGG GGGAAGCGCA GCGTCATTTT TGGGAACCTA
    GAGAAACTCT ATGACTTCCA CAGTCAGTAC TTCCTGCGAG AGCTCGAGAG CTGCTGCAAC
    CACCCGCTGC GCGTCAGCCA CTGCTTCCTG CGGCACAAAG ACCAGTTTGG GATGTACGCC
    TTATACAGCA AGAACAAGCC AAAGTCAGAC TCACTGCTGG CCAGCCATGG GAACACCTTC
    TTCAAGTTCA AGCAGGTGCA GCTGGGGGAT AAGATGGACC TGGCTTCCTA CCTGCTGAAA
    CCCATCCAGC GCATGAGCAA ATACGCCCTG CTCCTGAAGG ACCTGATCAA GGAGTGCAGT
    GAGGCCCAGG AGCAGGAGCT GGGCTACCTC CGGGCAGCAG AGGAGATGGT GAAGTTCCAG
    CTACGGCATG GGAATGACCT GCTGGCTATG GATGCCATCC GTGACTGTGA CGTGAACCTG
    AAGGAGCAGG GGCAGCTGGT ACGGCAGGAC GAGTTTGCCA TCTGGCTGGG CCGCAGGAAG
    TGCCAGCGCC ACGTCTTCCT CTTTGAGGAC CTGATCCTGT TCAGCAAGCC CAAGAGGATC
    GAGGGTGGCT TTGATGTGTA TATCTACAAG CGCTCCTTCA AGACTGCAGA CATCGGTCTG
    ACGGAAAACT CTGGCGACAG TGGTCTGCGT TTTGAGATCT GGTTCCGAAG GCGGAAGTCC
    AGCGACACTT ACATCCTCCA GGCCAGCTCA GCTGAGACCA AGCAGGCCTG GACCAGTGAC
    ATTGCCAAGA TCCTGTGGCA GCAGGCAGCC CGCAATAAAG GTGTGATTCC TTCCCAGGGC
    GTCTGTGCCG CAAAAGAAAT CCGCATGCAG GAGATGGTCT CCATGGGTGT GGGCAACAAG
    CCATTCCTGG ATATCAAACC CAGTGAGGCT GCTATCAATG ACCGTGCTAT TGACTACATC
    ATGAAAGGCA GAGCTGCCAG GACCAGAGCA TCAATTGCGG TGTCTCTCTT TGACCATTCC
    AACCCCTACA AGAGGACGCA GGCACAGCTT TCTGCTGGCA GCACCCCTGC TGCATATGGC
    CCATCCTCCT CCTCGCTGCT GGGGCCCCTC AACCTGCACA TGTACCTGGA CCAGGCACTG
    CTGCCTGGTG TCCTGGCACC CCGGCGCCCC TTTGATGTCA GCCCCTGCAT TGAGGAAGAT
    GAGCTGGAGC ATGAGACGAG CAGCCAGCCA TCACTGACCA CAGAGAGCTC CGAGTCATCA
    CACTGCATGT CAGGCAGCGG CTCCAGCGGC TCCGACAGTG GCTGTGTCTC CAGCATCCCA
    CAAGAAAGCT TCTGTGAGGA CATGGGTTCA CCTCCCTTCA TGCCCCTGGG CTCACAACAC
    AGCTCTGCGA TGGAGGGCAA ACCCCGGTTC ACGAACAGCC AGTACATTTC TGCAGTAAGT
    GCAAGCAGCC AAAGCACTCT CTTCGCTCTG CCCGTTCCCA CCACTGCCTC CCCAGTTCAA
    GGCACTGCCT CTCACGTGGG GACTGAAGAT GCTGCTTTTG GGCTGGGAAT TGCTGTGGGG
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40851
    Clone ID Related Accession (Same CDS sequence) XM_015279198.1 , XM_015279198.2
    Accession Version XM_015279198.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6243bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product puratrophin-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763868.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGACTCGG AGTCGCTGGA CGGCTGCATT CAGAGCACGT TATCAGCTCT CTACCCTCCG 
    TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC CAAGTTTTTG ATGTGGTGGA GAAGACGTAC
    CGTGGGGATG GACTGCGCTA CCTCATAGAC TTCCTGATTC CAGCCAAGCA CATCCTGCAG
    TGCATTCAGC AGGATGCCTG TGTTCAGTAC TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG
    CCCCTGTGCA TTCACGAGAA GATCGTAGTC CAGCTGGCTT CCATTGACTG GCGAATCTTG
    AAGCCTGGTG ACTTCTACCT GCAGGTGGTT CCCTATCTGA AGAAGTCTCC ACGGATTGTA
    TTGAAGTGTT TAGCAAAAGA CAAGCATAAT GTAGAAGAAG TTGTGATTCC AGAAGTTTCC
    TATACCTCTA TTTTTACTCT GGAATGGTTG AGTACTATCA ATGGAGAGCG GATGGGTATA
    GCACTGGAAA ATTGTTTACT AACCACTGAT GATAAAATAT TTCGTGTTCC CTGGGATAAC
    GTGGTTAATC CGGAATTTAT AAATAAACCA AAAATAACTG AAAATAATAT TATCACACCA
    GAGACAACAG GGAAACCTTC ACTTTTGTGT CTCCCCATGG ACCATGCTGA GCGCAGCTTG
    CCAGACGTAG AATCTCAGTA CCGACAGGAA CAAAGTCCAA ATTGCAACAA CCTGGTTGTT
    AGCAGCCCAG CTCCGCAGTT AGGTGAAACT CTTGTCAATG GTGTGTGTTC CAGTCCTGTT
    CCTAAAGAGG ATTTGCCAAG TGACCTTGAA GGGGAGTACG TTGAGCTTGC CGAAGTTTCG
    CTGCCCCGGT TTGGACCACA GACAGGCTCG TTAACCCAGT CACTAGCTTT AAATTATCTG
    ACTCAGCCTA GAACAAGAGT GAATGCATCT AAAGGCAAGC ACAGATTTAT TGTCATTCAA
    GATAGCACCT ACTCCAAGAA CTTAGTTCAC TCAGCACTTA TGCAAAAGGA GCACTGTCAG
    ATGGACACAC TGAGCACTTC TCCAGAAGTT CTCAAAAATG TAATTCCTTT GGAAAGCAAC
    AAAACGACTG CATGTGAGGA GCTGTCTCCA GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG
    AGCATGGGAA ACAGTTTTCC TGCAGCTGCA GCTCCTTCCT GCAGTGCAGA AGATTCCTGT
    GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC TCTGTTGATT GGCAGTATGC GCCGTGCAGC
    AACGGTGACG TGTGTGCTGG AGATGGTGTC AGCTGCAAAG CACAAATGAC TGGTTCTACT
    GGAAACATGG AATTAGAAAG CGATGGTTCT AGCATAAATC CCGGTGTTGA AATACTGGAG
    CAGAGCCCAG GAGCGAATCT AGATGCTGCT GCTGAAGAGG AGGTTATGGA AGAACACAGA
    GAACCACTGG CAGAGCATCA ACATGAGGTG ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC
    CTGGGGCCTT TGGGACCGTG CTGCACGCTG AAGGAAGGTC CCCATGCTTC TTGCCAGAGT
    GCTGGTGAAA GTACTATACC AGTCCACGCC ACTGCATTGC CTGCCACGCC TGAGAGTTTG
    GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA GGGAACGTGA CAGATGCAGC AGCAGTCCGA
    GCTTGTGCTG ATCAGGAAAC AAATTATAGT TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC
    GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG AGAAGAGAGA GCCCTGAGGA TGAAGAGATT
    GAACAAGTGC AGGCTGTGAA GGAGCATCGG AGTAGCTGTA GTTGCTGTTC TACCAACGCT
    CCTGCGGATG GAGTGCTTTC AGATGGTGAA GAACAAGAGG TCCCTGCTTG TCAGCATAAG
    AGAGCTGAAG ATGCCATGCT GCCCCAAACT GCACTGCCAG CAGAGAGTGG GCAGGTGGCA
    GAACAGTCAG GTACCAGCGG ATCCTTGCTG GGTCTTGATG CTCAAGCAGA AGACTCGAGT
    CAGTTTGGAA GCCACAGATC TGGAGAGAGG TGTGGGGATC CACGAGGATG GACAGAAAAC
    CAAGGCAATG GAAGTGAAAT GAACTTTGTG CTGAGCCCAG AGTGTGTTTC AGCACAGGAC
    TCGCAGGGAG GGGAGGAGAA CCAAGACAGT TTTTCTGATG TGGACAGGAC AAAGGCTGTA
    GCCTTAAAAA CACTGGAATC CATACAAGAG GAGCTGGAGG TTGGACCACT GTCCTCAGGA
    CCTGCTGAGG GGAACATAGA GCCCACTGCT TTGCATTCCC ACCCAGAGGC AGTTCCAGAA
    GCGTCATCTC CTAAAGGCAT GGTATCGGAC GGGGCAGAGG TGGTAAAAGA AGTATCTGGT
    GCAGATGTAT TGAAGCCAGC AGGTAAAGCC TGTGCCCTGG AGGAAACCTC AACAGCTGTT
    AGTCCCCCCA GATCCCCAGC TCCTGGGGCT GCTTGGGGCG GCCGGTCTGC ACCTTCCATA
    GTCAAGGATG TGAACCCTGA AGTGCTCAGG AGTGGAGTTG CCTGCCTGCC TGGTACCAGA
    GATAAATCGG GACGTGCAGT GGCGATAATA ACAACAAGGA ACACAGCCTG GTTAAACCCT
    CACTGCAACA CCACTGAGCT GGTTCGCCTC CTCCTGTACC TGCACAGCAT CCCAAGACCC
    GAGTGTCAGG CTTTGGGTCT GACTGTTCTC GTGGATGCTC GTCGCTGTTC GCCGGTTCCT
    GCTCTCTTCA AGGCATTCAG CATTTTGCAG GACATGGATC CTCATTGTAT TCATGGAGTA
    CTGCTTCTGG TTGAAAGAGA TCTGACTTTC CGGATGGAGA AGCCGACAGC AGGACAGTTT
    GAAATTCTCA CCTCCATGAA ATCCCTCCAT AAGCACATAG ACAGCTCACA GCTGCCTCTG
    GAACTGGACG GGACCTTCCC ATACTGCCAC CGGGACTGGT TAAGTTTCCG CATGAAACTG
    GAACACTTGC TACAGGGATG CCAAGGGGCT TGTGCCTTCC TCCAGGGAGC CATTCATAGA
    GTGGAATCTG GAAAATTACC AGAAAGAGCT GAGGAGGCTG CTGTGCTGCT GAGGAACTAC
    AGGCAGCTGA TGAAGAATGT TCTTGAGGAT GCACGGCTGG TCAGGCTTCA GCTGGAGGGT
    GGAGCACTGC TGGCCAGGCT GAGGAAGGAG GAGTCTTGTG TCACCCTCAC CCAGGACTAC
    AGGGACGCGC TTGATGCTGC TGGTGTGCTT TATAATCGAG TCGATGAGGG CGTTCACCGG
    CTGGTGATGG CGTCAAATAA ACGCATCCAG GAGCTGGAGC TGGTGATGGA GTTTGAGAAA
    GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT TGGATTGAGA ATGTTGGCAG AAGAAAGCTA
    AAGGAGATGA TCAACCTGGA CGATTCTTTG GAGATGCTGC TTCAAGCACA GAAGCAGTTC
    AGGGAGTTTG ATCTTGTTGC CAGTGAATAT TGCAGAAGGG GACAGGAAGC ACTTAAGAAG
    ATGGATCGTT GGGAAGACTT TTCCTCTGTG GATGTACATT CTTACAGAGT GAAGCTGCAG
    ACCTACAGAG ATCAACTGGA AGAATTCTGC ACTCAACTGG ATGAAAGCAG ACATCGAGTC
    TGTGAGACAG TCAGGCTGTA TGAATTCTTT GACAAGGCGT ACGAGTGGGC CTTGGAAGGG
    ATGCGACACC TCGCCTGCAT CAGCATGGAG GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG
    GTCATCAAGT GCCTGGAGAG CTACAAGGAA CAGCACCCAG ACATCAGCGA TGCCCGCTTC
    CAGGAGATGA AGGAGCTGGC CTGCGAACTG AAGAGCGACA AGGGACTCAA GCAGTGGAAA
    TTCGCGTGGT CCAAATGCCA AGAGACCAAG CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC
    TTGAGAACCA GGAGGGCACT GCTGTCGGAG CAGCCACAGG TGGGCAGCGA GGCTGGCAGC
    GTGTCCCACC GCAGGCACTC TGAGGGGGCT GCCCCCGGCC CACAGTGGGA CAGAACTCCT
    GGCACATCCC ACAGCCGGCC CAGTCGCTCC ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA
    CCCTCCCTGA GCTGCCCTGG TGATTTCGGT GCTGGGGAAG AATTTGCCTG CCAAGCTGCA
    CTCGGGGCCG GGCCTCACGT TGGCAGAGTT GCTTTTGGAA GCGGGGCCTG CAAGTCACCA
    AAACTGCCCG AGGAGAAGTC AAACCTGGAG AGCATCTTAG AAACGTTGGA GATGAAGCCA
    TCCTGTGCCT CCACACCTGC CTCTGGGAAG CTGCCTCCCA AGAGGATGCT CCGTAAGGCC
    CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC CTGTGGTCGG GCTGCCAGAG GACGCTGAGC
    GAGCCAGCCA GGTACGGCAA CACTGGTGTC TTCATCAAGG GACTGGAGGT GAGCAGCACG
    GAGCTGGTGG ACAGGACATT TGCACTGCGG CAGCTGGAGG GACAGCGTGG CTGTGGCTCT
    GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG CGGCACATCA TCGATGAGAT GGTGACCACG
    GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC ATCATTGAAA GCTACTTCCC TGAGATGGAG
    CGCCTGGATC TGCCCCAGGA CCTGCGGGGG AAGCGCAGCG TCATTTTTGG GAACCTAGAG
    AAACTCTATG ACTTCCACAG TCAGTACTTC CTGCGAGAGC TCGAGAGCTG CTGCAACCAC
    CCGCTGCGCG TCAGCCACTG CTTCCTGCGG CACAAAGACC AGTTTGGGAT GTACGCCTTA
    TACAGCAAGA ACAAGCCAAA GTCAGACTCA CTGCTGGCCA GCCATGGGAA CACCTTCTTC
    AAGTTCAAGC AGGTGCAGCT GGGGGATAAG ATGGACCTGG CTTCCTACCT GCTGAAACCC
    ATCCAGCGCA TGAGCAAATA CGCCCTGCTC CTGAAGGACC TGATCAAGGA GTGCAGTGAG
    GCCCAGGAGC AGGAGCTGGG CTACCTCCGG GCAGCAGAGG AGATGGTGAA GTTCCAGCTA
    CGGCATGGGA ATGACCTGCT GGCTATGGAT GCCATCCGTG ACTGTGACGT GAACCTGAAG
    GAGCAGGGGC AGCTGGTACG GCAGGACGAG TTTGCCATCT GGCTGGGCCG CAGGAAGTGC
    CAGCGCCACG TCTTCCTCTT TGAGGACCTG ATCCTGTTCA GCAAGCCCAA GAGGATCGAG
    GGTGGCTTTG ATGTGTATAT CTACAAGCGC TCCTTCAAGA CTGCAGACAT CGGTCTGACG
    GAAAACTCTG GCGACAGTGG TCTGCGTTTT GAGATCTGGT TCCGAAGGCG GAAGTCCAGC
    GACACTTACA TCCTCCAGGC CAGCTCAGCT GAGACCAAGC AGGCCTGGAC CAGTGACATT
    GCCAAGATCC TGTGGCAGCA GGCAGCCCGC AATAAAGGTG TGATTCCTTC CCAGGGCGTC
    TGTGCCGCAA AAGAAATCCG CATGCAGGAG ATGGTCTCCA TGGGTGTGGG CAACAAGCCA
    TTCCTGGATA TCAAACCCAG TGAGGCTGCT ATCAATGACC GTGCTATTGA CTACATCATG
    AAAGGCAGAG CTGCCAGGAC CAGAGCATCA ATTGCGGTGT CTCTCTTTGA CCATTCCAAC
    CCCTACAAGA GGACGCAGGC ACAGCTTTCT GCTGGCAGCA CCCCTGCTGC ATATGGCCCA
    TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC CTGCACATGT ACCTGGACCA GGCACTGCTG
    CCTGGTGTCC TGGCACCCCG GCGCCCCTTT GATGTCAGCC CCTGCATTGA GGAAGATGAG
    CTGGAGCATG AGACGAGCAG CCAGCCATCA CTGACCACAG AGAGCTCCGA GTCATCACAC
    TGCATGTCAG GCAGCGGCTC CAGCGGCTCC GACAGTGGCT GTGTCTCCAG CATCCCACAA
    GAAAGCTTCT GTGAGGACAT GGGTTCACCT CCCTTCATGC CCCTGGGCTC ACAACACAGC
    TCTGCGATGG AGGGCAAACC CCGGTTCACG AACAGCCAGT ACATTTCTGC AGTAAGTGCA
    AGCAGCCAAA GCACTCTCTT CGCTCTGCCC GTTCCCACCA CTGCCTCCCC AGTTCAAGGC
    ACTGCCTCTC ACGTGGGGAC TGAAGATGCT GCTTTTGGGC TGGGAATTGC TGTGGGGAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134684.1
    CDS664..6906
    Translation

    Target ORF information:

    RefSeq Version XM_015279198.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279198.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGACTCGG AGTCGCTGGA CGGCTGCATT CAGAGCACGT TATCAGCTCT CTACCCTCCG 
    TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC CAAGTTTTTG ATGTGGTGGA GAAGACGTAC
    CGTGGGGATG GACTGCGCTA CCTCATAGAC TTCCTGATTC CAGCCAAGCA CATCCTGCAG
    TGCATTCAGC AGGATGCCTG TGTTCAGTAC TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG
    CCCCTGTGCA TTCACGAGAA GATCGTAGTC CAGCTGGCTT CCATTGACTG GCGAATCTTG
    AAGCCTGGTG ACTTCTACCT GCAGGTGGTT CCCTATCTGA AGAAGTCTCC ACGGATTGTA
    TTGAAGTGTT TAGCAAAAGA CAAGCATAAT GTAGAAGAAG TTGTGATTCC AGAAGTTTCC
    TATACCTCTA TTTTTACTCT GGAATGGTTG AGTACTATCA ATGGAGAGCG GATGGGTATA
    GCACTGGAAA ATTGTTTACT AACCACTGAT GATAAAATAT TTCGTGTTCC CTGGGATAAC
    GTGGTTAATC CGGAATTTAT AAATAAACCA AAAATAACTG AAAATAATAT TATCACACCA
    GAGACAACAG GGAAACCTTC ACTTTTGTGT CTCCCCATGG ACCATGCTGA GCGCAGCTTG
    CCAGACGTAG AATCTCAGTA CCGACAGGAA CAAAGTCCAA ATTGCAACAA CCTGGTTGTT
    AGCAGCCCAG CTCCGCAGTT AGGTGAAACT CTTGTCAATG GTGTGTGTTC CAGTCCTGTT
    CCTAAAGAGG ATTTGCCAAG TGACCTTGAA GGGGAGTACG TTGAGCTTGC CGAAGTTTCG
    CTGCCCCGGT TTGGACCACA GACAGGCTCG TTAACCCAGT CACTAGCTTT AAATTATCTG
    ACTCAGCCTA GAACAAGAGT GAATGCATCT AAAGGCAAGC ACAGATTTAT TGTCATTCAA
    GATAGCACCT ACTCCAAGAA CTTAGTTCAC TCAGCACTTA TGCAAAAGGA GCACTGTCAG
    ATGGACACAC TGAGCACTTC TCCAGAAGTT CTCAAAAATG TAATTCCTTT GGAAAGCAAC
    AAAACGACTG CATGTGAGGA GCTGTCTCCA GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG
    AGCATGGGAA ACAGTTTTCC TGCAGCTGCA GCTCCTTCCT GCAGTGCAGA AGATTCCTGT
    GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC TCTGTTGATT GGCAGTATGC GCCGTGCAGC
    AACGGTGACG TGTGTGCTGG AGATGGTGTC AGCTGCAAAG CACAAATGAC TGGTTCTACT
    GGAAACATGG AATTAGAAAG CGATGGTTCT AGCATAAATC CCGGTGTTGA AATACTGGAG
    CAGAGCCCAG GAGCGAATCT AGATGCTGCT GCTGAAGAGG AGGTTATGGA AGAACACAGA
    GAACCACTGG CAGAGCATCA ACATGAGGTG ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC
    CTGGGGCCTT TGGGACCGTG CTGCACGCTG AAGGAAGGTC CCCATGCTTC TTGCCAGAGT
    GCTGGTGAAA GTACTATACC AGTCCACGCC ACTGCATTGC CTGCCACGCC TGAGAGTTTG
    GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA GGGAACGTGA CAGATGCAGC AGCAGTCCGA
    GCTTGTGCTG ATCAGGAAAC AAATTATAGT TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC
    GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG AGAAGAGAGA GCCCTGAGGA TGAAGAGATT
    GAACAAGTGC AGGCTGTGAA GGAGCATCGG AGTAGCTGTA GTTGCTGTTC TACCAACGCT
    CCTGCGGATG GAGTGCTTTC AGATGGTGAA GAACAAGAGG TCCCTGCTTG TCAGCATAAG
    AGAGCTGAAG ATGCCATGCT GCCCCAAACT GCACTGCCAG CAGAGAGTGG GCAGGTGGCA
    GAACAGTCAG GTACCAGCGG ATCCTTGCTG GGTCTTGATG CTCAAGCAGA AGACTCGAGT
    CAGTTTGGAA GCCACAGATC TGGAGAGAGG TGTGGGGATC CACGAGGATG GACAGAAAAC
    CAAGGCAATG GAAGTGAAAT GAACTTTGTG CTGAGCCCAG AGTGTGTTTC AGCACAGGAC
    TCGCAGGGAG GGGAGGAGAA CCAAGACAGT TTTTCTGATG TGGACAGGAC AAAGGCTGTA
    GCCTTAAAAA CACTGGAATC CATACAAGAG GAGCTGGAGG TTGGACCACT GTCCTCAGGA
    CCTGCTGAGG GGAACATAGA GCCCACTGCT TTGCATTCCC ACCCAGAGGC AGTTCCAGAA
    GCGTCATCTC CTAAAGGCAT GGTATCGGAC GGGGCAGAGG TGGTAAAAGA AGTATCTGGT
    GCAGATGTAT TGAAGCCAGC AGGTAAAGCC TGTGCCCTGG AGGAAACCTC AACAGCTGTT
    AGTCCCCCCA GATCCCCAGC TCCTGGGGCT GCTTGGGGCG GCCGGTCTGC ACCTTCCATA
    GTCAAGGATG TGAACCCTGA AGTGCTCAGG AGTGGAGTTG CCTGCCTGCC TGGTACCAGA
    GATAAATCGG GACGTGCAGT GGCGATAATA ACAACAAGGA ACACAGCCTG GTTAAACCCT
    CACTGCAACA CCACTGAGCT GGTTCGCCTC CTCCTGTACC TGCACAGCAT CCCAAGACCC
    GAGTGTCAGG CTTTGGGTCT GACTGTTCTC GTGGATGCTC GTCGCTGTTC GCCGGTTCCT
    GCTCTCTTCA AGGCATTCAG CATTTTGCAG GACATGGATC CTCATTGTAT TCATGGAGTA
    CTGCTTCTGG TTGAAAGAGA TCTGACTTTC CGGATGGAGA AGCCGACAGC AGGACAGTTT
    GAAATTCTCA CCTCCATGAA ATCCCTCCAT AAGCACATAG ACAGCTCACA GCTGCCTCTG
    GAACTGGACG GGACCTTCCC ATACTGCCAC CGGGACTGGT TAAGTTTCCG CATGAAACTG
    GAACACTTGC TACAGGGATG CCAAGGGGCT TGTGCCTTCC TCCAGGGAGC CATTCATAGA
    GTGGAATCTG GAAAATTACC AGAAAGAGCT GAGGAGGCTG CTGTGCTGCT GAGGAACTAC
    AGGCAGCTGA TGAAGAATGT TCTTGAGGAT GCACGGCTGG TCAGGCTTCA GCTGGAGGGT
    GGAGCACTGC TGGCCAGGCT GAGGAAGGAG GAGTCTTGTG TCACCCTCAC CCAGGACTAC
    AGGGACGCGC TTGATGCTGC TGGTGTGCTT TATAATCGAG TCGATGAGGG CGTTCACCGG
    CTGGTGATGG CGTCAAATAA ACGCATCCAG GAGCTGGAGC TGGTGATGGA GTTTGAGAAA
    GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT TGGATTGAGA ATGTTGGCAG AAGAAAGCTA
    AAGGAGATGA TCAACCTGGA CGATTCTTTG GAGATGCTGC TTCAAGCACA GAAGCAGTTC
    AGGGAGTTTG ATCTTGTTGC CAGTGAATAT TGCAGAAGGG GACAGGAAGC ACTTAAGAAG
    ATGGATCGTT GGGAAGACTT TTCCTCTGTG GATGTACATT CTTACAGAGT GAAGCTGCAG
    ACCTACAGAG ATCAACTGGA AGAATTCTGC ACTCAACTGG ATGAAAGCAG ACATCGAGTC
    TGTGAGACAG TCAGGCTGTA TGAATTCTTT GACAAGGCGT ACGAGTGGGC CTTGGAAGGG
    ATGCGACACC TCGCCTGCAT CAGCATGGAG GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG
    GTCATCAAGT GCCTGGAGAG CTACAAGGAA CAGCACCCAG ACATCAGCGA TGCCCGCTTC
    CAGGAGATGA AGGAGCTGGC CTGCGAACTG AAGAGCGACA AGGGACTCAA GCAGTGGAAA
    TTCGCGTGGT CCAAATGCCA AGAGACCAAG CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC
    TTGAGAACCA GGAGGGCACT GCTGTCGGAG CAGCCACAGG TGGGCAGCGA GGCTGGCAGC
    GTGTCCCACC GCAGGCACTC TGAGGGGGCT GCCCCCGGCC CACAGTGGGA CAGAACTCCT
    GGCACATCCC ACAGCCGGCC CAGTCGCTCC ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA
    CCCTCCCTGA GCTGCCCTGG TGATTTCGGT GCTGGGGAAG AATTTGCCTG CCAAGCTGCA
    CTCGGGGCCG GGCCTCACGT TGGCAGAGTT GCTTTTGGAA GCGGGGCCTG CAAGTCACCA
    AAACTGCCCG AGGAGAAGTC AAACCTGGAG AGCATCTTAG AAACGTTGGA GATGAAGCCA
    TCCTGTGCCT CCACACCTGC CTCTGGGAAG CTGCCTCCCA AGAGGATGCT CCGTAAGGCC
    CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC CTGTGGTCGG GCTGCCAGAG GACGCTGAGC
    GAGCCAGCCA GGTACGGCAA CACTGGTGTC TTCATCAAGG GACTGGAGGT GAGCAGCACG
    GAGCTGGTGG ACAGGACATT TGCACTGCGG CAGCTGGAGG GACAGCGTGG CTGTGGCTCT
    GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG CGGCACATCA TCGATGAGAT GGTGACCACG
    GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC ATCATTGAAA GCTACTTCCC TGAGATGGAG
    CGCCTGGATC TGCCCCAGGA CCTGCGGGGG AAGCGCAGCG TCATTTTTGG GAACCTAGAG
    AAACTCTATG ACTTCCACAG TCAGTACTTC CTGCGAGAGC TCGAGAGCTG CTGCAACCAC
    CCGCTGCGCG TCAGCCACTG CTTCCTGCGG CACAAAGACC AGTTTGGGAT GTACGCCTTA
    TACAGCAAGA ACAAGCCAAA GTCAGACTCA CTGCTGGCCA GCCATGGGAA CACCTTCTTC
    AAGTTCAAGC AGGTGCAGCT GGGGGATAAG ATGGACCTGG CTTCCTACCT GCTGAAACCC
    ATCCAGCGCA TGAGCAAATA CGCCCTGCTC CTGAAGGACC TGATCAAGGA GTGCAGTGAG
    GCCCAGGAGC AGGAGCTGGG CTACCTCCGG GCAGCAGAGG AGATGGTGAA GTTCCAGCTA
    CGGCATGGGA ATGACCTGCT GGCTATGGAT GCCATCCGTG ACTGTGACGT GAACCTGAAG
    GAGCAGGGGC AGCTGGTACG GCAGGACGAG TTTGCCATCT GGCTGGGCCG CAGGAAGTGC
    CAGCGCCACG TCTTCCTCTT TGAGGACCTG ATCCTGTTCA GCAAGCCCAA GAGGATCGAG
    GGTGGCTTTG ATGTGTATAT CTACAAGCGC TCCTTCAAGA CTGCAGACAT CGGTCTGACG
    GAAAACTCTG GCGACAGTGG TCTGCGTTTT GAGATCTGGT TCCGAAGGCG GAAGTCCAGC
    GACACTTACA TCCTCCAGGC CAGCTCAGCT GAGACCAAGC AGGCCTGGAC CAGTGACATT
    GCCAAGATCC TGTGGCAGCA GGCAGCCCGC AATAAAGGTG TGATTCCTTC CCAGGGCGTC
    TGTGCCGCAA AAGAAATCCG CATGCAGGAG ATGGTCTCCA TGGGTGTGGG CAACAAGCCA
    TTCCTGGATA TCAAACCCAG TGAGGCTGCT ATCAATGACC GTGCTATTGA CTACATCATG
    AAAGGCAGAG CTGCCAGGAC CAGAGCATCA ATTGCGGTGT CTCTCTTTGA CCATTCCAAC
    CCCTACAAGA GGACGCAGGC ACAGCTTTCT GCTGGCAGCA CCCCTGCTGC ATATGGCCCA
    TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC CTGCACATGT ACCTGGACCA GGCACTGCTG
    CCTGGTGTCC TGGCACCCCG GCGCCCCTTT GATGTCAGCC CCTGCATTGA GGAAGATGAG
    CTGGAGCATG AGACGAGCAG CCAGCCATCA CTGACCACAG AGAGCTCCGA GTCATCACAC
    TGCATGTCAG GCAGCGGCTC CAGCGGCTCC GACAGTGGCT GTGTCTCCAG CATCCCACAA
    GAAAGCTTCT GTGAGGACAT GGGTTCACCT CCCTTCATGC CCCTGGGCTC ACAACACAGC
    TCTGCGATGG AGGGCAAACC CCGGTTCACG AACAGCCAGT ACATTTCTGC AGTAAGTGCA
    AGCAGCCAAA GCACTCTCTT CGCTCTGCCC GTTCCCACCA CTGCCTCCCC AGTTCAAGGC
    ACTGCCTCTC ACGTGGGGAC TGAAGATGCT GCTTTTGGGC TGGGAATTGC TGTGGGGAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40851
    Clone ID Related Accession (Same CDS sequence) XM_015279198.1 , XM_015279198.2
    Accession Version XM_015279198.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6243bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product puratrophin-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006098.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279198.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGACTCGG AGTCGCTGGA CGGCTGCATT CAGAGCACGT TATCAGCTCT CTACCCTCCG 
    TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC CAAGTTTTTG ATGTGGTGGA GAAGACGTAC
    CGTGGGGATG GACTGCGCTA CCTCATAGAC TTCCTGATTC CAGCCAAGCA CATCCTGCAG
    TGCATTCAGC AGGATGCCTG TGTTCAGTAC TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG
    CCCCTGTGCA TTCACGAGAA GATCGTAGTC CAGCTGGCTT CCATTGACTG GCGAATCTTG
    AAGCCTGGTG ACTTCTACCT GCAGGTGGTT CCCTATCTGA AGAAGTCTCC ACGGATTGTA
    TTGAAGTGTT TAGCAAAAGA CAAGCATAAT GTAGAAGAAG TTGTGATTCC AGAAGTTTCC
    TATACCTCTA TTTTTACTCT GGAATGGTTG AGTACTATCA ATGGAGAGCG GATGGGTATA
    GCACTGGAAA ATTGTTTACT AACCACTGAT GATAAAATAT TTCGTGTTCC CTGGGATAAC
    GTGGTTAATC CGGAATTTAT AAATAAACCA AAAATAACTG AAAATAATAT TATCACACCA
    GAGACAACAG GGAAACCTTC ACTTTTGTGT CTCCCCATGG ACCATGCTGA GCGCAGCTTG
    CCAGACGTAG AATCTCAGTA CCGACAGGAA CAAAGTCCAA ATTGCAACAA CCTGGTTGTT
    AGCAGCCCAG CTCCGCAGTT AGGTGAAACT CTTGTCAATG GTGTGTGTTC CAGTCCTGTT
    CCTAAAGAGG ATTTGCCAAG TGACCTTGAA GGGGAGTACG TTGAGCTTGC CGAAGTTTCG
    CTGCCCCGGT TTGGACCACA GACAGGCTCG TTAACCCAGT CACTAGCTTT AAATTATCTG
    ACTCAGCCTA GAACAAGAGT GAATGCATCT AAAGGCAAGC ACAGATTTAT TGTCATTCAA
    GATAGCACCT ACTCCAAGAA CTTAGTTCAC TCAGCACTTA TGCAAAAGGA GCACTGTCAG
    ATGGACACAC TGAGCACTTC TCCAGAAGTT CTCAAAAATG TAATTCCTTT GGAAAGCAAC
    AAAACGACTG CATGTGAGGA GCTGTCTCCA GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG
    AGCATGGGAA ACAGTTTTCC TGCAGCTGCA GCTCCTTCCT GCAGTGCAGA AGATTCCTGT
    GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC TCTGTTGATT GGCAGTATGC GCCGTGCAGC
    AACGGTGACG TGTGTGCTGG AGATGGTGTC AGCTGCAAAG CACAAATGAC TGGTTCTACT
    GGAAACATGG AATTAGAAAG CGATGGTTCT AGCATAAATC CCGGTGTTGA AATACTGGAG
    CAGAGCCCAG GAGCGAATCT AGATGCTGCT GCTGAAGAGG AGGTTATGGA AGAACACAGA
    GAACCACTGG CAGAGCATCA ACATGAGGTG ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC
    CTGGGGCCTT TGGGACCGTG CTGCACGCTG AAGGAAGGTC CCCATGCTTC TTGCCAGAGT
    GCTGGTGAAA GTACTATACC AGTCCACGCC ACTGCATTGC CTGCCACGCC TGAGAGTTTG
    GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA GGGAACGTGA CAGATGCAGC AGCAGTCCGA
    GCTTGTGCTG ATCAGGAAAC AAATTATAGT TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC
    GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG AGAAGAGAGA GCCCTGAGGA TGAAGAGATT
    GAACAAGTGC AGGCTGTGAA GGAGCATCGG AGTAGCTGTA GTTGCTGTTC TACCAACGCT
    CCTGCGGATG GAGTGCTTTC AGATGGTGAA GAACAAGAGG TCCCTGCTTG TCAGCATAAG
    AGAGCTGAAG ATGCCATGCT GCCCCAAACT GCACTGCCAG CAGAGAGTGG GCAGGTGGCA
    GAACAGTCAG GTACCAGCGG ATCCTTGCTG GGTCTTGATG CTCAAGCAGA AGACTCGAGT
    CAGTTTGGAA GCCACAGATC TGGAGAGAGG TGTGGGGATC CACGAGGATG GACAGAAAAC
    CAAGGCAATG GAAGTGAAAT GAACTTTGTG CTGAGCCCAG AGTGTGTTTC AGCACAGGAC
    TCGCAGGGAG GGGAGGAGAA CCAAGACAGT TTTTCTGATG TGGACAGGAC AAAGGCTGTA
    GCCTTAAAAA CACTGGAATC CATACAAGAG GAGCTGGAGG TTGGACCACT GTCCTCAGGA
    CCTGCTGAGG GGAACATAGA GCCCACTGCT TTGCATTCCC ACCCAGAGGC AGTTCCAGAA
    GCGTCATCTC CTAAAGGCAT GGTATCGGAC GGGGCAGAGG TGGTAAAAGA AGTATCTGGT
    GCAGATGTAT TGAAGCCAGC AGGTAAAGCC TGTGCCCTGG AGGAAACCTC AACAGCTGTT
    AGTCCCCCCA GATCCCCAGC TCCTGGGGCT GCTTGGGGCG GCCGGTCTGC ACCTTCCATA
    GTCAAGGATG TGAACCCTGA AGTGCTCAGG AGTGGAGTTG CCTGCCTGCC TGGTACCAGA
    GATAAATCGG GACGTGCAGT GGCGATAATA ACAACAAGGA ACACAGCCTG GTTAAACCCT
    CACTGCAACA CCACTGAGCT GGTTCGCCTC CTCCTGTACC TGCACAGCAT CCCAAGACCC
    GAGTGTCAGG CTTTGGGTCT GACTGTTCTC GTGGATGCTC GTCGCTGTTC GCCGGTTCCT
    GCTCTCTTCA AGGCATTCAG CATTTTGCAG GACATGGATC CTCATTGTAT TCATGGAGTA
    CTGCTTCTGG TTGAAAGAGA TCTGACTTTC CGGATGGAGA AGCCGACAGC AGGACAGTTT
    GAAATTCTCA CCTCCATGAA ATCCCTCCAT AAGCACATAG ACAGCTCACA GCTGCCTCTG
    GAACTGGACG GGACCTTCCC ATACTGCCAC CGGGACTGGT TAAGTTTCCG CATGAAACTG
    GAACACTTGC TACAGGGATG CCAAGGGGCT TGTGCCTTCC TCCAGGGAGC CATTCATAGA
    GTGGAATCTG GAAAATTACC AGAAAGAGCT GAGGAGGCTG CTGTGCTGCT GAGGAACTAC
    AGGCAGCTGA TGAAGAATGT TCTTGAGGAT GCACGGCTGG TCAGGCTTCA GCTGGAGGGT
    GGAGCACTGC TGGCCAGGCT GAGGAAGGAG GAGTCTTGTG TCACCCTCAC CCAGGACTAC
    AGGGACGCGC TTGATGCTGC TGGTGTGCTT TATAATCGAG TCGATGAGGG CGTTCACCGG
    CTGGTGATGG CGTCAAATAA ACGCATCCAG GAGCTGGAGC TGGTGATGGA GTTTGAGAAA
    GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT TGGATTGAGA ATGTTGGCAG AAGAAAGCTA
    AAGGAGATGA TCAACCTGGA CGATTCTTTG GAGATGCTGC TTCAAGCACA GAAGCAGTTC
    AGGGAGTTTG ATCTTGTTGC CAGTGAATAT TGCAGAAGGG GACAGGAAGC ACTTAAGAAG
    ATGGATCGTT GGGAAGACTT TTCCTCTGTG GATGTACATT CTTACAGAGT GAAGCTGCAG
    ACCTACAGAG ATCAACTGGA AGAATTCTGC ACTCAACTGG ATGAAAGCAG ACATCGAGTC
    TGTGAGACAG TCAGGCTGTA TGAATTCTTT GACAAGGCGT ACGAGTGGGC CTTGGAAGGG
    ATGCGACACC TCGCCTGCAT CAGCATGGAG GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG
    GTCATCAAGT GCCTGGAGAG CTACAAGGAA CAGCACCCAG ACATCAGCGA TGCCCGCTTC
    CAGGAGATGA AGGAGCTGGC CTGCGAACTG AAGAGCGACA AGGGACTCAA GCAGTGGAAA
    TTCGCGTGGT CCAAATGCCA AGAGACCAAG CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC
    TTGAGAACCA GGAGGGCACT GCTGTCGGAG CAGCCACAGG TGGGCAGCGA GGCTGGCAGC
    GTGTCCCACC GCAGGCACTC TGAGGGGGCT GCCCCCGGCC CACAGTGGGA CAGAACTCCT
    GGCACATCCC ACAGCCGGCC CAGTCGCTCC ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA
    CCCTCCCTGA GCTGCCCTGG TGATTTCGGT GCTGGGGAAG AATTTGCCTG CCAAGCTGCA
    CTCGGGGCCG GGCCTCACGT TGGCAGAGTT GCTTTTGGAA GCGGGGCCTG CAAGTCACCA
    AAACTGCCCG AGGAGAAGTC AAACCTGGAG AGCATCTTAG AAACGTTGGA GATGAAGCCA
    TCCTGTGCCT CCACACCTGC CTCTGGGAAG CTGCCTCCCA AGAGGATGCT CCGTAAGGCC
    CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC CTGTGGTCGG GCTGCCAGAG GACGCTGAGC
    GAGCCAGCCA GGTACGGCAA CACTGGTGTC TTCATCAAGG GACTGGAGGT GAGCAGCACG
    GAGCTGGTGG ACAGGACATT TGCACTGCGG CAGCTGGAGG GACAGCGTGG CTGTGGCTCT
    GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG CGGCACATCA TCGATGAGAT GGTGACCACG
    GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC ATCATTGAAA GCTACTTCCC TGAGATGGAG
    CGCCTGGATC TGCCCCAGGA CCTGCGGGGG AAGCGCAGCG TCATTTTTGG GAACCTAGAG
    AAACTCTATG ACTTCCACAG TCAGTACTTC CTGCGAGAGC TCGAGAGCTG CTGCAACCAC
    CCGCTGCGCG TCAGCCACTG CTTCCTGCGG CACAAAGACC AGTTTGGGAT GTACGCCTTA
    TACAGCAAGA ACAAGCCAAA GTCAGACTCA CTGCTGGCCA GCCATGGGAA CACCTTCTTC
    AAGTTCAAGC AGGTGCAGCT GGGGGATAAG ATGGACCTGG CTTCCTACCT GCTGAAACCC
    ATCCAGCGCA TGAGCAAATA CGCCCTGCTC CTGAAGGACC TGATCAAGGA GTGCAGTGAG
    GCCCAGGAGC AGGAGCTGGG CTACCTCCGG GCAGCAGAGG AGATGGTGAA GTTCCAGCTA
    CGGCATGGGA ATGACCTGCT GGCTATGGAT GCCATCCGTG ACTGTGACGT GAACCTGAAG
    GAGCAGGGGC AGCTGGTACG GCAGGACGAG TTTGCCATCT GGCTGGGCCG CAGGAAGTGC
    CAGCGCCACG TCTTCCTCTT TGAGGACCTG ATCCTGTTCA GCAAGCCCAA GAGGATCGAG
    GGTGGCTTTG ATGTGTATAT CTACAAGCGC TCCTTCAAGA CTGCAGACAT CGGTCTGACG
    GAAAACTCTG GCGACAGTGG TCTGCGTTTT GAGATCTGGT TCCGAAGGCG GAAGTCCAGC
    GACACTTACA TCCTCCAGGC CAGCTCAGCT GAGACCAAGC AGGCCTGGAC CAGTGACATT
    GCCAAGATCC TGTGGCAGCA GGCAGCCCGC AATAAAGGTG TGATTCCTTC CCAGGGCGTC
    TGTGCCGCAA AAGAAATCCG CATGCAGGAG ATGGTCTCCA TGGGTGTGGG CAACAAGCCA
    TTCCTGGATA TCAAACCCAG TGAGGCTGCT ATCAATGACC GTGCTATTGA CTACATCATG
    AAAGGCAGAG CTGCCAGGAC CAGAGCATCA ATTGCGGTGT CTCTCTTTGA CCATTCCAAC
    CCCTACAAGA GGACGCAGGC ACAGCTTTCT GCTGGCAGCA CCCCTGCTGC ATATGGCCCA
    TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC CTGCACATGT ACCTGGACCA GGCACTGCTG
    CCTGGTGTCC TGGCACCCCG GCGCCCCTTT GATGTCAGCC CCTGCATTGA GGAAGATGAG
    CTGGAGCATG AGACGAGCAG CCAGCCATCA CTGACCACAG AGAGCTCCGA GTCATCACAC
    TGCATGTCAG GCAGCGGCTC CAGCGGCTCC GACAGTGGCT GTGTCTCCAG CATCCCACAA
    GAAAGCTTCT GTGAGGACAT GGGTTCACCT CCCTTCATGC CCCTGGGCTC ACAACACAGC
    TCTGCGATGG AGGGCAAACC CCGGTTCACG AACAGCCAGT ACATTTCTGC AGTAAGTGCA
    AGCAGCCAAA GCACTCTCTT CGCTCTGCCC GTTCCCACCA CTGCCTCCCC AGTTCAAGGC
    ACTGCCTCTC ACGTGGGGAC TGAAGATGCT GCTTTTGGGC TGGGAATTGC TGTGGGGAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015134684.1
    CDS665..6907
    Translation

    Target ORF information:

    RefSeq Version XM_015279198.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279198.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGACTCGG AGTCGCTGGA CGGCTGCATT CAGAGCACGT TATCAGCTCT CTACCCTCCG 
    TTCGAGGCTA CGGCCGCCAC CGTGCTGTGC CAAGTTTTTG ATGTGGTGGA GAAGACGTAC
    CGTGGGGATG GACTGCGCTA CCTCATAGAC TTCCTGATTC CAGCCAAGCA CATCCTGCAG
    TGCATTCAGC AGGATGCCTG TGTTCAGTAC TGTGGCTTGC TGTTTCGCCA CGAGGGCTGG
    CCCCTGTGCA TTCACGAGAA GATCGTAGTC CAGCTGGCTT CCATTGACTG GCGAATCTTG
    AAGCCTGGTG ACTTCTACCT GCAGGTGGTT CCCTATCTGA AGAAGTCTCC ACGGATTGTA
    TTGAAGTGTT TAGCAAAAGA CAAGCATAAT GTAGAAGAAG TTGTGATTCC AGAAGTTTCC
    TATACCTCTA TTTTTACTCT GGAATGGTTG AGTACTATCA ATGGAGAGCG GATGGGTATA
    GCACTGGAAA ATTGTTTACT AACCACTGAT GATAAAATAT TTCGTGTTCC CTGGGATAAC
    GTGGTTAATC CGGAATTTAT AAATAAACCA AAAATAACTG AAAATAATAT TATCACACCA
    GAGACAACAG GGAAACCTTC ACTTTTGTGT CTCCCCATGG ACCATGCTGA GCGCAGCTTG
    CCAGACGTAG AATCTCAGTA CCGACAGGAA CAAAGTCCAA ATTGCAACAA CCTGGTTGTT
    AGCAGCCCAG CTCCGCAGTT AGGTGAAACT CTTGTCAATG GTGTGTGTTC CAGTCCTGTT
    CCTAAAGAGG ATTTGCCAAG TGACCTTGAA GGGGAGTACG TTGAGCTTGC CGAAGTTTCG
    CTGCCCCGGT TTGGACCACA GACAGGCTCG TTAACCCAGT CACTAGCTTT AAATTATCTG
    ACTCAGCCTA GAACAAGAGT GAATGCATCT AAAGGCAAGC ACAGATTTAT TGTCATTCAA
    GATAGCACCT ACTCCAAGAA CTTAGTTCAC TCAGCACTTA TGCAAAAGGA GCACTGTCAG
    ATGGACACAC TGAGCACTTC TCCAGAAGTT CTCAAAAATG TAATTCCTTT GGAAAGCAAC
    AAAACGACTG CATGTGAGGA GCTGTCTCCA GAAGGTCAGC TGCTGTCAGG TGGTCCTGGG
    AGCATGGGAA ACAGTTTTCC TGCAGCTGCA GCTCCTTCCT GCAGTGCAGA AGATTCCTGT
    GCTGCAGAGC AGGAGGCCTG GAGTGAGTGC TCTGTTGATT GGCAGTATGC GCCGTGCAGC
    AACGGTGACG TGTGTGCTGG AGATGGTGTC AGCTGCAAAG CACAAATGAC TGGTTCTACT
    GGAAACATGG AATTAGAAAG CGATGGTTCT AGCATAAATC CCGGTGTTGA AATACTGGAG
    CAGAGCCCAG GAGCGAATCT AGATGCTGCT GCTGAAGAGG AGGTTATGGA AGAACACAGA
    GAACCACTGG CAGAGCATCA ACATGAGGTG ACGCAGGTGG ATCCCTCTCT TGGGTCTGTC
    CTGGGGCCTT TGGGACCGTG CTGCACGCTG AAGGAAGGTC CCCATGCTTC TTGCCAGAGT
    GCTGGTGAAA GTACTATACC AGTCCACGCC ACTGCATTGC CTGCCACGCC TGAGAGTTTG
    GCTGTGGGTG TGGAGAGAAG CTCTCCTTCA GGGAACGTGA CAGATGCAGC AGCAGTCCGA
    GCTTGTGCTG ATCAGGAAAC AAATTATAGT TGTCCAGTAA ATCCTCCAGA GCAGTTGAAC
    GGAGGAGAAG TAGGTGAAAT AGAGCTGGGG AGAAGAGAGA GCCCTGAGGA TGAAGAGATT
    GAACAAGTGC AGGCTGTGAA GGAGCATCGG AGTAGCTGTA GTTGCTGTTC TACCAACGCT
    CCTGCGGATG GAGTGCTTTC AGATGGTGAA GAACAAGAGG TCCCTGCTTG TCAGCATAAG
    AGAGCTGAAG ATGCCATGCT GCCCCAAACT GCACTGCCAG CAGAGAGTGG GCAGGTGGCA
    GAACAGTCAG GTACCAGCGG ATCCTTGCTG GGTCTTGATG CTCAAGCAGA AGACTCGAGT
    CAGTTTGGAA GCCACAGATC TGGAGAGAGG TGTGGGGATC CACGAGGATG GACAGAAAAC
    CAAGGCAATG GAAGTGAAAT GAACTTTGTG CTGAGCCCAG AGTGTGTTTC AGCACAGGAC
    TCGCAGGGAG GGGAGGAGAA CCAAGACAGT TTTTCTGATG TGGACAGGAC AAAGGCTGTA
    GCCTTAAAAA CACTGGAATC CATACAAGAG GAGCTGGAGG TTGGACCACT GTCCTCAGGA
    CCTGCTGAGG GGAACATAGA GCCCACTGCT TTGCATTCCC ACCCAGAGGC AGTTCCAGAA
    GCGTCATCTC CTAAAGGCAT GGTATCGGAC GGGGCAGAGG TGGTAAAAGA AGTATCTGGT
    GCAGATGTAT TGAAGCCAGC AGGTAAAGCC TGTGCCCTGG AGGAAACCTC AACAGCTGTT
    AGTCCCCCCA GATCCCCAGC TCCTGGGGCT GCTTGGGGCG GCCGGTCTGC ACCTTCCATA
    GTCAAGGATG TGAACCCTGA AGTGCTCAGG AGTGGAGTTG CCTGCCTGCC TGGTACCAGA
    GATAAATCGG GACGTGCAGT GGCGATAATA ACAACAAGGA ACACAGCCTG GTTAAACCCT
    CACTGCAACA CCACTGAGCT GGTTCGCCTC CTCCTGTACC TGCACAGCAT CCCAAGACCC
    GAGTGTCAGG CTTTGGGTCT GACTGTTCTC GTGGATGCTC GTCGCTGTTC GCCGGTTCCT
    GCTCTCTTCA AGGCATTCAG CATTTTGCAG GACATGGATC CTCATTGTAT TCATGGAGTA
    CTGCTTCTGG TTGAAAGAGA TCTGACTTTC CGGATGGAGA AGCCGACAGC AGGACAGTTT
    GAAATTCTCA CCTCCATGAA ATCCCTCCAT AAGCACATAG ACAGCTCACA GCTGCCTCTG
    GAACTGGACG GGACCTTCCC ATACTGCCAC CGGGACTGGT TAAGTTTCCG CATGAAACTG
    GAACACTTGC TACAGGGATG CCAAGGGGCT TGTGCCTTCC TCCAGGGAGC CATTCATAGA
    GTGGAATCTG GAAAATTACC AGAAAGAGCT GAGGAGGCTG CTGTGCTGCT GAGGAACTAC
    AGGCAGCTGA TGAAGAATGT TCTTGAGGAT GCACGGCTGG TCAGGCTTCA GCTGGAGGGT
    GGAGCACTGC TGGCCAGGCT GAGGAAGGAG GAGTCTTGTG TCACCCTCAC CCAGGACTAC
    AGGGACGCGC TTGATGCTGC TGGTGTGCTT TATAATCGAG TCGATGAGGG CGTTCACCGG
    CTGGTGATGG CGTCAAATAA ACGCATCCAG GAGCTGGAGC TGGTGATGGA GTTTGAGAAA
    GTGGAAGAAT GTTTTAAGGA GGTCAGCAGT TGGATTGAGA ATGTTGGCAG AAGAAAGCTA
    AAGGAGATGA TCAACCTGGA CGATTCTTTG GAGATGCTGC TTCAAGCACA GAAGCAGTTC
    AGGGAGTTTG ATCTTGTTGC CAGTGAATAT TGCAGAAGGG GACAGGAAGC ACTTAAGAAG
    ATGGATCGTT GGGAAGACTT TTCCTCTGTG GATGTACATT CTTACAGAGT GAAGCTGCAG
    ACCTACAGAG ATCAACTGGA AGAATTCTGC ACTCAACTGG ATGAAAGCAG ACATCGAGTC
    TGTGAGACAG TCAGGCTGTA TGAATTCTTT GACAAGGCGT ACGAGTGGGC CTTGGAAGGG
    ATGCGACACC TCGCCTGCAT CAGCATGGAG GAGTGCAGCT CCGCCGAGCA CTGCGCCGGG
    GTCATCAAGT GCCTGGAGAG CTACAAGGAA CAGCACCCAG ACATCAGCGA TGCCCGCTTC
    CAGGAGATGA AGGAGCTGGC CTGCGAACTG AAGAGCGACA AGGGACTCAA GCAGTGGAAA
    TTCGCGTGGT CCAAATGCCA AGAGACCAAG CTGGTTTTTG AAAAGAAGCT GGAAGCAGCC
    TTGAGAACCA GGAGGGCACT GCTGTCGGAG CAGCCACAGG TGGGCAGCGA GGCTGGCAGC
    GTGTCCCACC GCAGGCACTC TGAGGGGGCT GCCCCCGGCC CACAGTGGGA CAGAACTCCT
    GGCACATCCC ACAGCCGGCC CAGTCGCTCC ACGGGGTGGA CTAAAGAGAA AGCTCCCTCA
    CCCTCCCTGA GCTGCCCTGG TGATTTCGGT GCTGGGGAAG AATTTGCCTG CCAAGCTGCA
    CTCGGGGCCG GGCCTCACGT TGGCAGAGTT GCTTTTGGAA GCGGGGCCTG CAAGTCACCA
    AAACTGCCCG AGGAGAAGTC AAACCTGGAG AGCATCTTAG AAACGTTGGA GATGAAGCCA
    TCCTGTGCCT CCACACCTGC CTCTGGGAAG CTGCCTCCCA AGAGGATGCT CCGTAAGGCC
    CAAAGCTTTG ACCTGCCCTG CGGTGAGAGC CTGTGGTCGG GCTGCCAGAG GACGCTGAGC
    GAGCCAGCCA GGTACGGCAA CACTGGTGTC TTCATCAAGG GACTGGAGGT GAGCAGCACG
    GAGCTGGTGG ACAGGACATT TGCACTGCGG CAGCTGGAGG GACAGCGTGG CTGTGGCTCT
    GCAGAGGCCA GGAGCTGGGG CAGCAAGCTG CGGCACATCA TCGATGAGAT GGTGACCACG
    GAGCGGGAGT ACGTCAGGTC GCTGTGCTAC ATCATTGAAA GCTACTTCCC TGAGATGGAG
    CGCCTGGATC TGCCCCAGGA CCTGCGGGGG AAGCGCAGCG TCATTTTTGG GAACCTAGAG
    AAACTCTATG ACTTCCACAG TCAGTACTTC CTGCGAGAGC TCGAGAGCTG CTGCAACCAC
    CCGCTGCGCG TCAGCCACTG CTTCCTGCGG CACAAAGACC AGTTTGGGAT GTACGCCTTA
    TACAGCAAGA ACAAGCCAAA GTCAGACTCA CTGCTGGCCA GCCATGGGAA CACCTTCTTC
    AAGTTCAAGC AGGTGCAGCT GGGGGATAAG ATGGACCTGG CTTCCTACCT GCTGAAACCC
    ATCCAGCGCA TGAGCAAATA CGCCCTGCTC CTGAAGGACC TGATCAAGGA GTGCAGTGAG
    GCCCAGGAGC AGGAGCTGGG CTACCTCCGG GCAGCAGAGG AGATGGTGAA GTTCCAGCTA
    CGGCATGGGA ATGACCTGCT GGCTATGGAT GCCATCCGTG ACTGTGACGT GAACCTGAAG
    GAGCAGGGGC AGCTGGTACG GCAGGACGAG TTTGCCATCT GGCTGGGCCG CAGGAAGTGC
    CAGCGCCACG TCTTCCTCTT TGAGGACCTG ATCCTGTTCA GCAAGCCCAA GAGGATCGAG
    GGTGGCTTTG ATGTGTATAT CTACAAGCGC TCCTTCAAGA CTGCAGACAT CGGTCTGACG
    GAAAACTCTG GCGACAGTGG TCTGCGTTTT GAGATCTGGT TCCGAAGGCG GAAGTCCAGC
    GACACTTACA TCCTCCAGGC CAGCTCAGCT GAGACCAAGC AGGCCTGGAC CAGTGACATT
    GCCAAGATCC TGTGGCAGCA GGCAGCCCGC AATAAAGGTG TGATTCCTTC CCAGGGCGTC
    TGTGCCGCAA AAGAAATCCG CATGCAGGAG ATGGTCTCCA TGGGTGTGGG CAACAAGCCA
    TTCCTGGATA TCAAACCCAG TGAGGCTGCT ATCAATGACC GTGCTATTGA CTACATCATG
    AAAGGCAGAG CTGCCAGGAC CAGAGCATCA ATTGCGGTGT CTCTCTTTGA CCATTCCAAC
    CCCTACAAGA GGACGCAGGC ACAGCTTTCT GCTGGCAGCA CCCCTGCTGC ATATGGCCCA
    TCCTCCTCCT CGCTGCTGGG GCCCCTCAAC CTGCACATGT ACCTGGACCA GGCACTGCTG
    CCTGGTGTCC TGGCACCCCG GCGCCCCTTT GATGTCAGCC CCTGCATTGA GGAAGATGAG
    CTGGAGCATG AGACGAGCAG CCAGCCATCA CTGACCACAG AGAGCTCCGA GTCATCACAC
    TGCATGTCAG GCAGCGGCTC CAGCGGCTCC GACAGTGGCT GTGTCTCCAG CATCCCACAA
    GAAAGCTTCT GTGAGGACAT GGGTTCACCT CCCTTCATGC CCCTGGGCTC ACAACACAGC
    TCTGCGATGG AGGGCAAACC CCGGTTCACG AACAGCCAGT ACATTTCTGC AGTAAGTGCA
    AGCAGCCAAA GCACTCTCTT CGCTCTGCCC GTTCCCACCA CTGCCTCCCC AGTTCAAGGC
    ACTGCCTCTC ACGTGGGGAC TGAAGATGCT GCTTTTGGGC TGGGAATTGC TGTGGGGAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.