plekhg4 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009303345.3	XP_009301620.1	uncharacterized protein plekhg4 isoform X2
XM_005169007.3	XP_005169064.1	puratrophin-1 isoform X3
XM_009303344.3	XP_009301619.1	uncharacterized protein plekhg4 isoform X1
XM_009303345.2	XP_009301620.1	uncharacterized protein plekhg4 isoform X2
XM_021477915.1	XP_021333590.1	puratrophin-1 isoform X3
XM_005169007.4	XP_005169064.1	puratrophin-1 isoform X4
XM_021471995.1	XP_021327670.1	pleckstrin homology domain-containing family G member 4B isoform X6
XM_021471996.1	XP_021327671.1	puratrophin-1 isoform X4
XM_009303344.2	XP_009301619.1	uncharacterized protein plekhg4 isoform X1
XM_021471994.1	XP_021327669.1	pleckstrin homology domain-containing family G member 4B isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa13239	XM_005169007.4 Latest version!	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa13239	XM_021471996.1 Latest version!	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa144922	XM_021471994.1 Latest version!	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa144923	XM_021471995.1 Latest version!	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa143496	XM_021477915.1 Latest version!	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa39866	XM_009303344.3 Latest version!	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa39867	XM_009303345.3 Latest version!	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
View Old Accession Versions >>
ODa39867	XM_009303345.2	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa13239	XM_005169007.3	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa39866	XM_009303344.2	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
Hide Old Accession Versions >>

plekhg4 ( XM_005169007.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169064.1 Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa13239
Clone ID Related Accession (Same CDS sequence)	XM_005169007.3 , XM_005169007.4 , XM_021471996.1
Accession Version	XM_005169007.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	puratrophin-1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169007.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCCG CTGCTCCGTG CTCCGGTCAT GGGACCACCG GCGTCTTAGC CGAGCAGGAA 
AATGGATATA GCCATGGAGA GGCATATGAA TGGGCTCTAG AGGGCATGCG TCATTTGGCG 
TGTGTCAGTA TGGAGGATTG TGGCATGTCT GAAAAGTGTC AGAGCGTGAT CACGTGTTTG 
GAGACTTACC GCAGTCAGCA CCCCCCGATC CCTGATGCAC ACTTCCAGGA GATGAAGGAC 
CTCGCTGGGG AACTGAAAAG CGAACAGGGC CTCAAGCAGT GGAAGTTTGC CTGGTCCAAG 
TGTCAGGAGA CCAAGCAGAT GTTTGAAAAG AAGCTGGAGA TGGCCCTGCG TTCCCGCCGT 
GACAGCGACT CCAAGTCAGT AAGGAGTGAC TCTTCTCGTC GTAACTCAGA TGTTAGCACT 
AAACACCAAG AGCGTAGCAG TAGCATCTCC TTCTCATGTC GGAAAGCGTT CGGCGGAGCC 
TGGGGTCGAG ATAGACTTTC CTCACATTCT AGCACATCCT CAACTCCCAG TAGCCCTTCA 
GGCATTCGTC TGGATGCCCG TGACACTGTC CGAACTCCAA CAGGAAGTCC ATACAGCATT 
GAGCACAGAA TCCCCCACAG CACCCCTGTC AGATCCCACA GGCTAACACG TAGCATCTCC 
ACAGATGAGT CTCCTCAGAG CCCACATGCA GAGGGGCTAG CGTCCACAAG CCCAAGCGGC 
ACTTCTGTTC AAACAAACCG CAGGGTTTTG CGGAAGACCC AGAGCTTCGA TACAGCTGGC 
AGTGAGCCTG TGTCACAGTA CGGGACCTGT CAGAGGACTT TGAGTGAGCC TGCACGCAGA 
GGAAACACAG GGGTGTTCAT TAAGGGTCTG GAGGTGAGCA GCACTGAAGT GATTGATAGG 
CCGTACAGTC CACGACTACC ACCCATCCAT GGATGGTCTT CAATTGATGC ACACCGCAGC 
GGGAGTCCTG CTCCAGAAAC GCGCAGCAAG GGCAGCAAGT TACGACACAT TGTAGATGAG 
ATGGTGACAA CAGAGCGGGA ATATGTGCGT TCCCTGCGGT ATATCATTGA CAACTATTTC 
CCTGAGATGG AGCGCGCTGT CCTGCCGCAG GATCTGCGGG GAAAGCGGAG CGTCATATTT 
GGAAATCTGG AGAAACTGGT GGACTTTCAT AGTCAGTATT TCCTAAAAGA GCTGGAGAGC 
TGCTGTAATC ATCCTCTACG CGTCAGCCAC TGTTTCCTAC GACATCAAGA CCAGTTTAGC 
CTCTATGCAT TATACAGCAA GAACAAGCCA AAATCAGACA CACTCCTGGC CAGCCATGGA 
AACAACTTCT TCAAGCATAA GCAGATGGAG CTGGGAGATA AGATGGACCT GGCATCGTAC 
CTGCTGAAGC CCATCCAGCG CATGAGTAAA TACGCTCTTC TGCTGAAAGA CCTGATCAAA 
GAAGTGAGCG AGGTCCAGGA GCAGGAACTT ACATACCTGC GAGCCGCAGC AGAGATGGTC 
AAGTTTCAGC TCCGCCATGG CAATGATCTA CTCGCCATGG ACGCTATCAG AGATTGTGAT 
GTGAACCTAA AAGAGCAAGG ACAGTTGGTT CGTCAAGATG AGTTCACGAT TTCATATGGC 
AGAAAGAAGT GCCAGAGACA CGTGTTCCTA TTTGAGGACC TAGTGCTCTT CAGCAAACCC 
AAGCGCATCG AGGGTGGACT AGATGTTTAC ATATACAAGC ATTCTTTTAA GACGGCTGAT 
GTGGGTATGA CAGAGACGTC AGGTGAGAAC AGTCTGAGGT TTGAAATTTG GTTTCGGAGG 
AGAACTTCAA AGAACCAGAC CTACATTCTC CAGTCCAGCA CTGCTGACAT CAAACATGCC 
TGGACTTGCG ACATCGCACG AATACTGTGG CAGCAGGCCA CGCGAAACAA AGAAATTCGA 
ATGCAGGAGA TGGTCTCAAT GGGTGTGGGC AATAAACCCT TCCTGGACAT TAAACCCAGT 
GATGCTGCAA TCAACGACAG AGCCATCGAC TACATTATGA AGGGCAGAGG AGCTAGAACG 
AGAGCCTCCA TTGCTGTTTC TCTGTTTGAC CACTCAAACC CATTCAAAAG AGCTGCAGTA 
AACACCCCCG TCAGCGGGAC CCCGGTGTCT GGCGGTCCTT CCTCATCCAC CCTGCTGGGG 
CCCTTGAATC TGCACATGTA CAGCAGCCAA AGTCTGTTGG CGGGGGAGAG ACCTCTGATT 
GGCCCCTGCA TCGAGGAGGA CGAGCTGGAG CATGAGACCA GTAGTCAGCC CTCTATGACT 
ACAGAGAGTT CGGGTTCGTC GTCTCACTGT CTGTCTGGCA GTGGCTCTAG CGGATCAGAC 
AGCGGCTGTG TGTCCAGTCA CCTTCCTGAG GCTTTGTCTG AGGAGCCCAG CTCACCTTGT 
GACTCCTCGT GTTATTCCTC CATCACCTCT CCCACGCAGG AGAAGCCCTG CTTTAACAGC 
CAGTATATCT CAGCGGGGGA TGCTCAGGTG ATCGGACCCT CTACTATAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169064.1
CDS	94..2607
Translation	MTAAAPCSGHGTTGVLAEQENGYSHGEAYEWALEGMRHLACVSMEDCGMSEKCQSVITCLETYRSQHPPIPDAHFQEMKDLAGELKSEQGLKQWKFAWSKCQETKQMFEKKLEMALRSRRDSDSKSVRSDSSRRNSDVSTKHQERSSSISFSCRKAFGGAWGRDRLSSHSSTSSTPSSPSGIRLDARDTVRTPTGSPYSIEHRIPHSTPVRSHRLTRSISTDESPQSPHAEGLASTSPSGTSVQTNRRVLRKTQSFDTAGSEPVSQYGTCQRTLSEPARRGNTGVFIKGLEVSSTEVIDRPYSPRLPPIHGWSSIDAHRSGSPAPETRSKGSKLRHIVDEMVTTEREYVRSLRYIIDNYFPEMERAVLPQDLRGKRSVIFGNLEKLVDFHSQYFLKELESCCNHPLRVSHCFLRHQDQFSLYALYSKNKPKSDTLLASHGNNFFKHKQMELGDKMDLASYLLKPIQRMSKYALLLKDLIKEVSEVQEQELTYLRAAAEMVKFQLRHGNDLLAMDAIRDCDVNLKEQGQLVRQDEFTISYGRKKCQRHVFLFEDLVLFSKPKRIEGGLDVYIYKHSFKTADVGMTETSGENSLRFEIWFRRRTSKNQTYILQSSTADIKHAWTCDIARILWQQATRNKEIRMQEMVSMGVGNKPFLDIKPSDAAINDRAIDYIMKGRGARTRASIAVSLFDHSNPFKRAAVNTPVSGTPVSGGPSSSTLLGPLNLHMYSSQSLLAGERPLIGPCIEEDELEHETSSQPSMTTESSGSSSHCLSGSGSSGSDSGCVSSHLPEALSEEPSSPCDSSCYSSITSPTQEKPCFNSQYISAGDAQVIGPSTIV

Target ORF information:

RefSeq Version	XM_005169007.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169007.3

ORF Insert Sequence:

ATGACCGCCG CTGCTCCGTG CTCCGGTCAT GGGACCACCG GCGTCTTAGC CGAGCAGGAA 
AATGGATATA GCCATGGAGA GGCATATGAA TGGGCTCTAG AGGGCATGCG TCATTTGGCG 
TGTGTCAGTA TGGAGGATTG TGGCATGTCT GAAAAGTGTC AGAGCGTGAT CACGTGTTTG 
GAGACTTACC GCAGTCAGCA CCCCCCGATC CCTGATGCAC ACTTCCAGGA GATGAAGGAC 
CTCGCTGGGG AACTGAAAAG CGAACAGGGC CTCAAGCAGT GGAAGTTTGC CTGGTCCAAG 
TGTCAGGAGA CCAAGCAGAT GTTTGAAAAG AAGCTGGAGA TGGCCCTGCG TTCCCGCCGT 
GACAGCGACT CCAAGTCAGT AAGGAGTGAC TCTTCTCGTC GTAACTCAGA TGTTAGCACT 
AAACACCAAG AGCGTAGCAG TAGCATCTCC TTCTCATGTC GGAAAGCGTT CGGCGGAGCC 
TGGGGTCGAG ATAGACTTTC CTCACATTCT AGCACATCCT CAACTCCCAG TAGCCCTTCA 
GGCATTCGTC TGGATGCCCG TGACACTGTC CGAACTCCAA CAGGAAGTCC ATACAGCATT 
GAGCACAGAA TCCCCCACAG CACCCCTGTC AGATCCCACA GGCTAACACG TAGCATCTCC 
ACAGATGAGT CTCCTCAGAG CCCACATGCA GAGGGGCTAG CGTCCACAAG CCCAAGCGGC 
ACTTCTGTTC AAACAAACCG CAGGGTTTTG CGGAAGACCC AGAGCTTCGA TACAGCTGGC 
AGTGAGCCTG TGTCACAGTA CGGGACCTGT CAGAGGACTT TGAGTGAGCC TGCACGCAGA 
GGAAACACAG GGGTGTTCAT TAAGGGTCTG GAGGTGAGCA GCACTGAAGT GATTGATAGG 
CCGTACAGTC CACGACTACC ACCCATCCAT GGATGGTCTT CAATTGATGC ACACCGCAGC 
GGGAGTCCTG CTCCAGAAAC GCGCAGCAAG GGCAGCAAGT TACGACACAT TGTAGATGAG 
ATGGTGACAA CAGAGCGGGA ATATGTGCGT TCCCTGCGGT ATATCATTGA CAACTATTTC 
CCTGAGATGG AGCGCGCTGT CCTGCCGCAG GATCTGCGGG GAAAGCGGAG CGTCATATTT 
GGAAATCTGG AGAAACTGGT GGACTTTCAT AGTCAGTATT TCCTAAAAGA GCTGGAGAGC 
TGCTGTAATC ATCCTCTACG CGTCAGCCAC TGTTTCCTAC GACATCAAGA CCAGTTTAGC 
CTCTATGCAT TATACAGCAA GAACAAGCCA AAATCAGACA CACTCCTGGC CAGCCATGGA 
AACAACTTCT TCAAGCATAA GCAGATGGAG CTGGGAGATA AGATGGACCT GGCATCGTAC 
CTGCTGAAGC CCATCCAGCG CATGAGTAAA TACGCTCTTC TGCTGAAAGA CCTGATCAAA 
GAAGTGAGCG AGGTCCAGGA GCAGGAACTT ACATACCTGC GAGCCGCAGC AGAGATGGTC 
AAGTTTCAGC TCCGCCATGG CAATGATCTA CTCGCCATGG ACGCTATCAG AGATTGTGAT 
GTGAACCTAA AAGAGCAAGG ACAGTTGGTT CGTCAAGATG AGTTCACGAT TTCATATGGC 
AGAAAGAAGT GCCAGAGACA CGTGTTCCTA TTTGAGGACC TAGTGCTCTT CAGCAAACCC 
AAGCGCATCG AGGGTGGACT AGATGTTTAC ATATACAAGC ATTCTTTTAA GACGGCTGAT 
GTGGGTATGA CAGAGACGTC AGGTGAGAAC AGTCTGAGGT TTGAAATTTG GTTTCGGAGG 
AGAACTTCAA AGAACCAGAC CTACATTCTC CAGTCCAGCA CTGCTGACAT CAAACATGCC 
TGGACTTGCG ACATCGCACG AATACTGTGG CAGCAGGCCA CGCGAAACAA AGAAATTCGA 
ATGCAGGAGA TGGTCTCAAT GGGTGTGGGC AATAAACCCT TCCTGGACAT TAAACCCAGT 
GATGCTGCAA TCAACGACAG AGCCATCGAC TACATTATGA AGGGCAGAGG AGCTAGAACG 
AGAGCCTCCA TTGCTGTTTC TCTGTTTGAC CACTCAAACC CATTCAAAAG AGCTGCAGTA 
AACACCCCCG TCAGCGGGAC CCCGGTGTCT GGCGGTCCTT CCTCATCCAC CCTGCTGGGG 
CCCTTGAATC TGCACATGTA CAGCAGCCAA AGTCTGTTGG CGGGGGAGAG ACCTCTGATT 
GGCCCCTGCA TCGAGGAGGA CGAGCTGGAG CATGAGACCA GTAGTCAGCC CTCTATGACT 
ACAGAGAGTT CGGGTTCGTC GTCTCACTGT CTGTCTGGCA GTGGCTCTAG CGGATCAGAC 
AGCGGCTGTG TGTCCAGTCA CCTTCCTGAG GCTTTGTCTG AGGAGCCCAG CTCACCTTGT 
GACTCCTCGT GTTATTCCTC CATCACCTCT CCCACGCAGG AGAAGCCCTG CTTTAACAGC 
CAGTATATCT CAGCGGGGGA TGCTCAGGTG ATCGGACCCT CTACTATAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg4 ( XM_005169007.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169064.1 Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa13239
Clone ID Related Accession (Same CDS sequence)	XM_005169007.3 , XM_005169007.4 , XM_021471996.1
Accession Version	XM_005169007.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	puratrophin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169007.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCCG CTGCTCCGTG CTCCGGTCAT GGGACCACCG GCGTCTTAGC CGAGCAGGAA 
AATGGATATA GCCATGGAGA GGCATATGAA TGGGCTCTAG AGGGCATGCG TCATTTGGCG 
TGTGTCAGTA TGGAGGATTG TGGCATGTCT GAAAAGTGTC AGAGCGTGAT CACGTGTTTG 
GAGACTTACC GCAGTCAGCA CCCCCCGATC CCTGATGCAC ACTTCCAGGA GATGAAGGAC 
CTCGCTGGGG AACTGAAAAG CGAACAGGGC CTCAAGCAGT GGAAGTTTGC CTGGTCCAAG 
TGTCAGGAGA CCAAGCAGAT GTTTGAAAAG AAGCTGGAGA TGGCCCTGCG TTCCCGCCGT 
GACAGCGACT CCAAGTCAGT AAGGAGTGAC TCTTCTCGTC GTAACTCAGA TGTTAGCACT 
AAACACCAAG AGCGTAGCAG TAGCATCTCC TTCTCATGTC GGAAAGCGTT CGGCGGAGCC 
TGGGGTCGAG ATAGACTTTC CTCACATTCT AGCACATCCT CAACTCCCAG TAGCCCTTCA 
GGCATTCGTC TGGATGCCCG TGACACTGTC CGAACTCCAA CAGGAAGTCC ATACAGCATT 
GAGCACAGAA TCCCCCACAG CACCCCTGTC AGATCCCACA GGCTAACACG TAGCATCTCC 
ACAGATGAGT CTCCTCAGAG CCCACATGCA GAGGGGCTAG CGTCCACAAG CCCAAGCGGC 
ACTTCTGTTC AAACAAACCG CAGGGTTTTG CGGAAGACCC AGAGCTTCGA TACAGCTGGC 
AGTGAGCCTG TGTCACAGTA CGGGACCTGT CAGAGGACTT TGAGTGAGCC TGCACGCAGA 
GGAAACACAG GGGTGTTCAT TAAGGGTCTG GAGGTGAGCA GCACTGAAGT GATTGATAGG 
CCGTACAGTC CACGACTACC ACCCATCCAT GGATGGTCTT CAATTGATGC ACACCGCAGC 
GGGAGTCCTG CTCCAGAAAC GCGCAGCAAG GGCAGCAAGT TACGACACAT TGTAGATGAG 
ATGGTGACAA CAGAGCGGGA ATATGTGCGT TCCCTGCGGT ATATCATTGA CAACTATTTC 
CCTGAGATGG AGCGCGCTGT CCTGCCGCAG GATCTGCGGG GAAAGCGGAG CGTCATATTT 
GGAAATCTGG AGAAACTGGT GGACTTTCAT AGTCAGTATT TCCTAAAAGA GCTGGAGAGC 
TGCTGTAATC ATCCTCTACG CGTCAGCCAC TGTTTCCTAC GACATCAAGA CCAGTTTAGC 
CTCTATGCAT TATACAGCAA GAACAAGCCA AAATCAGACA CACTCCTGGC CAGCCATGGA 
AACAACTTCT TCAAGCATAA GCAGATGGAG CTGGGAGATA AGATGGACCT GGCATCGTAC 
CTGCTGAAGC CCATCCAGCG CATGAGTAAA TACGCTCTTC TGCTGAAAGA CCTGATCAAA 
GAAGTGAGCG AGGTCCAGGA GCAGGAACTT ACATACCTGC GAGCCGCAGC AGAGATGGTC 
AAGTTTCAGC TCCGCCATGG CAATGATCTA CTCGCCATGG ACGCTATCAG AGATTGTGAT 
GTGAACCTAA AAGAGCAAGG ACAGTTGGTT CGTCAAGATG AGTTCACGAT TTCATATGGC 
AGAAAGAAGT GCCAGAGACA CGTGTTCCTA TTTGAGGACC TAGTGCTCTT CAGCAAACCC 
AAGCGCATCG AGGGTGGACT AGATGTTTAC ATATACAAGC ATTCTTTTAA GACGGCTGAT 
GTGGGTATGA CAGAGACGTC AGGTGAGAAC AGTCTGAGGT TTGAAATTTG GTTTCGGAGG 
AGAACTTCAA AGAACCAGAC CTACATTCTC CAGTCCAGCA CTGCTGACAT CAAACATGCC 
TGGACTTGCG ACATCGCACG AATACTGTGG CAGCAGGCCA CGCGAAACAA AGAAATTCGA 
ATGCAGGAGA TGGTCTCAAT GGGTGTGGGC AATAAACCCT TCCTGGACAT TAAACCCAGT 
GATGCTGCAA TCAACGACAG AGCCATCGAC TACATTATGA AGGGCAGAGG AGCTAGAACG 
AGAGCCTCCA TTGCTGTTTC TCTGTTTGAC CACTCAAACC CATTCAAAAG AGCTGCAGTA 
AACACCCCCG TCAGCGGGAC CCCGGTGTCT GGCGGTCCTT CCTCATCCAC CCTGCTGGGG 
CCCTTGAATC TGCACATGTA CAGCAGCCAA AGTCTGTTGG CGGGGGAGAG ACCTCTGATT 
GGCCCCTGCA TCGAGGAGGA CGAGCTGGAG CATGAGACCA GTAGTCAGCC CTCTATGACT 
ACAGAGAGTT CGGGTTCGTC GTCTCACTGT CTGTCTGGCA GTGGCTCTAG CGGATCAGAC 
AGCGGCTGTG TGTCCAGTCA CCTTCCTGAG GCTTTGTCTG AGGAGCCCAG CTCACCTTGT 
GACTCCTCGT GTTATTCCTC CATCACCTCT CCCACGCAGG AGAAGCCCTG CTTTAACAGC 
CAGTATATCT CAGCGGGGGA TGCTCAGGTG ATCGGACCCT CTACTATAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169064.1
CDS	95..2608
Translation	MTAAAPCSGHGTTGVLAEQENGYSHGEAYEWALEGMRHLACVSMEDCGMSEKCQSVITCLETYRSQHPPIPDAHFQEMKDLAGELKSEQGLKQWKFAWSKCQETKQMFEKKLEMALRSRRDSDSKSVRSDSSRRNSDVSTKHQERSSSISFSCRKAFGGAWGRDRLSSHSSTSSTPSSPSGIRLDARDTVRTPTGSPYSIEHRIPHSTPVRSHRLTRSISTDESPQSPHAEGLASTSPSGTSVQTNRRVLRKTQSFDTAGSEPVSQYGTCQRTLSEPARRGNTGVFIKGLEVSSTEVIDRPYSPRLPPIHGWSSIDAHRSGSPAPETRSKGSKLRHIVDEMVTTEREYVRSLRYIIDNYFPEMERAVLPQDLRGKRSVIFGNLEKLVDFHSQYFLKELESCCNHPLRVSHCFLRHQDQFSLYALYSKNKPKSDTLLASHGNNFFKHKQMELGDKMDLASYLLKPIQRMSKYALLLKDLIKEVSEVQEQELTYLRAAAEMVKFQLRHGNDLLAMDAIRDCDVNLKEQGQLVRQDEFTISYGRKKCQRHVFLFEDLVLFSKPKRIEGGLDVYIYKHSFKTADVGMTETSGENSLRFEIWFRRRTSKNQTYILQSSTADIKHAWTCDIARILWQQATRNKEIRMQEMVSMGVGNKPFLDIKPSDAAINDRAIDYIMKGRGARTRASIAVSLFDHSNPFKRAAVNTPVSGTPVSGGPSSSTLLGPLNLHMYSSQSLLAGERPLIGPCIEEDELEHETSSQPSMTTESSGSSSHCLSGSGSSGSDSGCVSSHLPEALSEEPSSPCDSSCYSSITSPTQEKPCFNSQYISAGDAQVIGPSTIV

Target ORF information:

RefSeq Version	XM_005169007.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169007.4

ORF Insert Sequence:

ATGACCGCCG CTGCTCCGTG CTCCGGTCAT GGGACCACCG GCGTCTTAGC CGAGCAGGAA 
AATGGATATA GCCATGGAGA GGCATATGAA TGGGCTCTAG AGGGCATGCG TCATTTGGCG 
TGTGTCAGTA TGGAGGATTG TGGCATGTCT GAAAAGTGTC AGAGCGTGAT CACGTGTTTG 
GAGACTTACC GCAGTCAGCA CCCCCCGATC CCTGATGCAC ACTTCCAGGA GATGAAGGAC 
CTCGCTGGGG AACTGAAAAG CGAACAGGGC CTCAAGCAGT GGAAGTTTGC CTGGTCCAAG 
TGTCAGGAGA CCAAGCAGAT GTTTGAAAAG AAGCTGGAGA TGGCCCTGCG TTCCCGCCGT 
GACAGCGACT CCAAGTCAGT AAGGAGTGAC TCTTCTCGTC GTAACTCAGA TGTTAGCACT 
AAACACCAAG AGCGTAGCAG TAGCATCTCC TTCTCATGTC GGAAAGCGTT CGGCGGAGCC 
TGGGGTCGAG ATAGACTTTC CTCACATTCT AGCACATCCT CAACTCCCAG TAGCCCTTCA 
GGCATTCGTC TGGATGCCCG TGACACTGTC CGAACTCCAA CAGGAAGTCC ATACAGCATT 
GAGCACAGAA TCCCCCACAG CACCCCTGTC AGATCCCACA GGCTAACACG TAGCATCTCC 
ACAGATGAGT CTCCTCAGAG CCCACATGCA GAGGGGCTAG CGTCCACAAG CCCAAGCGGC 
ACTTCTGTTC AAACAAACCG CAGGGTTTTG CGGAAGACCC AGAGCTTCGA TACAGCTGGC 
AGTGAGCCTG TGTCACAGTA CGGGACCTGT CAGAGGACTT TGAGTGAGCC TGCACGCAGA 
GGAAACACAG GGGTGTTCAT TAAGGGTCTG GAGGTGAGCA GCACTGAAGT GATTGATAGG 
CCGTACAGTC CACGACTACC ACCCATCCAT GGATGGTCTT CAATTGATGC ACACCGCAGC 
GGGAGTCCTG CTCCAGAAAC GCGCAGCAAG GGCAGCAAGT TACGACACAT TGTAGATGAG 
ATGGTGACAA CAGAGCGGGA ATATGTGCGT TCCCTGCGGT ATATCATTGA CAACTATTTC 
CCTGAGATGG AGCGCGCTGT CCTGCCGCAG GATCTGCGGG GAAAGCGGAG CGTCATATTT 
GGAAATCTGG AGAAACTGGT GGACTTTCAT AGTCAGTATT TCCTAAAAGA GCTGGAGAGC 
TGCTGTAATC ATCCTCTACG CGTCAGCCAC TGTTTCCTAC GACATCAAGA CCAGTTTAGC 
CTCTATGCAT TATACAGCAA GAACAAGCCA AAATCAGACA CACTCCTGGC CAGCCATGGA 
AACAACTTCT TCAAGCATAA GCAGATGGAG CTGGGAGATA AGATGGACCT GGCATCGTAC 
CTGCTGAAGC CCATCCAGCG CATGAGTAAA TACGCTCTTC TGCTGAAAGA CCTGATCAAA 
GAAGTGAGCG AGGTCCAGGA GCAGGAACTT ACATACCTGC GAGCCGCAGC AGAGATGGTC 
AAGTTTCAGC TCCGCCATGG CAATGATCTA CTCGCCATGG ACGCTATCAG AGATTGTGAT 
GTGAACCTAA AAGAGCAAGG ACAGTTGGTT CGTCAAGATG AGTTCACGAT TTCATATGGC 
AGAAAGAAGT GCCAGAGACA CGTGTTCCTA TTTGAGGACC TAGTGCTCTT CAGCAAACCC 
AAGCGCATCG AGGGTGGACT AGATGTTTAC ATATACAAGC ATTCTTTTAA GACGGCTGAT 
GTGGGTATGA CAGAGACGTC AGGTGAGAAC AGTCTGAGGT TTGAAATTTG GTTTCGGAGG 
AGAACTTCAA AGAACCAGAC CTACATTCTC CAGTCCAGCA CTGCTGACAT CAAACATGCC 
TGGACTTGCG ACATCGCACG AATACTGTGG CAGCAGGCCA CGCGAAACAA AGAAATTCGA 
ATGCAGGAGA TGGTCTCAAT GGGTGTGGGC AATAAACCCT TCCTGGACAT TAAACCCAGT 
GATGCTGCAA TCAACGACAG AGCCATCGAC TACATTATGA AGGGCAGAGG AGCTAGAACG 
AGAGCCTCCA TTGCTGTTTC TCTGTTTGAC CACTCAAACC CATTCAAAAG AGCTGCAGTA 
AACACCCCCG TCAGCGGGAC CCCGGTGTCT GGCGGTCCTT CCTCATCCAC CCTGCTGGGG 
CCCTTGAATC TGCACATGTA CAGCAGCCAA AGTCTGTTGG CGGGGGAGAG ACCTCTGATT 
GGCCCCTGCA TCGAGGAGGA CGAGCTGGAG CATGAGACCA GTAGTCAGCC CTCTATGACT 
ACAGAGAGTT CGGGTTCGTC GTCTCACTGT CTGTCTGGCA GTGGCTCTAG CGGATCAGAC 
AGCGGCTGTG TGTCCAGTCA CCTTCCTGAG GCTTTGTCTG AGGAGCCCAG CTCACCTTGT 
GACTCCTCGT GTTATTCCTC CATCACCTCT CCCACGCAGG AGAAGCCCTG CTTTAACAGC 
CAGTATATCT CAGCGGGGGA TGCTCAGGTG ATCGGACCCT CTACTATAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg4 ( XM_021471996.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327671.1 Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa13239
Clone ID Related Accession (Same CDS sequence)	XM_005169007.3 , XM_005169007.4 , XM_021471996.1
Accession Version	XM_021471996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	puratrophin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394699.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCCG CTGCTCCGTG CTCCGGTCAT GGGACCACCG GCGTCTTAGC CGAGCAGGAA 
AATGGATATA GCCATGGAGA GGCATATGAA TGGGCTCTAG AGGGCATGCG TCATTTGGCG 
TGTGTCAGTA TGGAGGATTG TGGCATGTCT GAAAAGTGTC AGAGCGTGAT CACGTGTTTG 
GAGACTTACC GCAGTCAGCA CCCCCCGATC CCTGATGCAC ACTTCCAGGA GATGAAGGAC 
CTCGCTGGGG AACTGAAAAG CGAACAGGGC CTCAAGCAGT GGAAGTTTGC CTGGTCCAAG 
TGTCAGGAGA CCAAGCAGAT GTTTGAAAAG AAGCTGGAGA TGGCCCTGCG TTCCCGCCGT 
GACAGCGACT CCAAGTCAGT AAGGAGTGAC TCTTCTCGTC GTAACTCAGA TGTTAGCACT 
AAACACCAAG AGCGTAGCAG TAGCATCTCC TTCTCATGTC GGAAAGCGTT CGGCGGAGCC 
TGGGGTCGAG ATAGACTTTC CTCACATTCT AGCACATCCT CAACTCCCAG TAGCCCTTCA 
GGCATTCGTC TGGATGCCCG TGACACTGTC CGAACTCCAA CAGGAAGTCC ATACAGCATT 
GAGCACAGAA TCCCCCACAG CACCCCTGTC AGATCCCACA GGCTAACACG TAGCATCTCC 
ACAGATGAGT CTCCTCAGAG CCCACATGCA GAGGGGCTAG CGTCCACAAG CCCAAGCGGC 
ACTTCTGTTC AAACAAACCG CAGGGTTTTG CGGAAGACCC AGAGCTTCGA TACAGCTGGC 
AGTGAGCCTG TGTCACAGTA CGGGACCTGT CAGAGGACTT TGAGTGAGCC TGCACGCAGA 
GGAAACACAG GGGTGTTCAT TAAGGGTCTG GAGGTGAGCA GCACTGAAGT GATTGATAGG 
CCGTACAGTC CACGACTACC ACCCATCCAT GGATGGTCTT CAATTGATGC ACACCGCAGC 
GGGAGTCCTG CTCCAGAAAC GCGCAGCAAG GGCAGCAAGT TACGACACAT TGTAGATGAG 
ATGGTGACAA CAGAGCGGGA ATATGTGCGT TCCCTGCGGT ATATCATTGA CAACTATTTC 
CCTGAGATGG AGCGCGCTGT CCTGCCGCAG GATCTGCGGG GAAAGCGGAG CGTCATATTT 
GGAAATCTGG AGAAACTGGT GGACTTTCAT AGTCAGTATT TCCTAAAAGA GCTGGAGAGC 
TGCTGTAATC ATCCTCTACG CGTCAGCCAC TGTTTCCTAC GACATCAAGA CCAGTTTAGC 
CTCTATGCAT TATACAGCAA GAACAAGCCA AAATCAGACA CACTCCTGGC CAGCCATGGA 
AACAACTTCT TCAAGCATAA GCAGATGGAG CTGGGAGATA AGATGGACCT GGCATCGTAC 
CTGCTGAAGC CCATCCAGCG CATGAGTAAA TACGCTCTTC TGCTGAAAGA CCTGATCAAA 
GAAGTGAGCG AGGTCCAGGA GCAGGAACTT ACATACCTGC GAGCCGCAGC AGAGATGGTC 
AAGTTTCAGC TCCGCCATGG CAATGATCTA CTCGCCATGG ACGCTATCAG AGATTGTGAT 
GTGAACCTAA AAGAGCAAGG ACAGTTGGTT CGTCAAGATG AGTTCACGAT TTCATATGGC 
AGAAAGAAGT GCCAGAGACA CGTGTTCCTA TTTGAGGACC TAGTGCTCTT CAGCAAACCC 
AAGCGCATCG AGGGTGGACT AGATGTTTAC ATATACAAGC ATTCTTTTAA GACGGCTGAT 
GTGGGTATGA CAGAGACGTC AGGTGAGAAC AGTCTGAGGT TTGAAATTTG GTTTCGGAGG 
AGAACTTCAA AGAACCAGAC CTACATTCTC CAGTCCAGCA CTGCTGACAT CAAACATGCC 
TGGACTTGCG ACATCGCACG AATACTGTGG CAGCAGGCCA CGCGAAACAA AGAAATTCGA 
ATGCAGGAGA TGGTCTCAAT GGGTGTGGGC AATAAACCCT TCCTGGACAT TAAACCCAGT 
GATGCTGCAA TCAACGACAG AGCCATCGAC TACATTATGA AGGGCAGAGG AGCTAGAACG 
AGAGCCTCCA TTGCTGTTTC TCTGTTTGAC CACTCAAACC CATTCAAAAG AGCTGCAGTA 
AACACCCCCG TCAGCGGGAC CCCGGTGTCT GGCGGTCCTT CCTCATCCAC CCTGCTGGGG 
CCCTTGAATC TGCACATGTA CAGCAGCCAA AGTCTGTTGG CGGGGGAGAG ACCTCTGATT 
GGCCCCTGCA TCGAGGAGGA CGAGCTGGAG CATGAGACCA GTAGTCAGCC CTCTATGACT 
ACAGAGAGTT CGGGTTCGTC GTCTCACTGT CTGTCTGGCA GTGGCTCTAG CGGATCAGAC 
AGCGGCTGTG TGTCCAGTCA CCTTCCTGAG GCTTTGTCTG AGGAGCCCAG CTCACCTTGT 
GACTCCTCGT GTTATTCCTC CATCACCTCT CCCACGCAGG AGAAGCCCTG CTTTAACAGC 
CAGTATATCT CAGCGGGGGA TGCTCAGGTG ATCGGACCCT CTACTATAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327671.1
CDS	95..2608
Translation	MTAAAPCSGHGTTGVLAEQENGYSHGEAYEWALEGMRHLACVSMEDCGMSEKCQSVITCLETYRSQHPPIPDAHFQEMKDLAGELKSEQGLKQWKFAWSKCQETKQMFEKKLEMALRSRRDSDSKSVRSDSSRRNSDVSTKHQERSSSISFSCRKAFGGAWGRDRLSSHSSTSSTPSSPSGIRLDARDTVRTPTGSPYSIEHRIPHSTPVRSHRLTRSISTDESPQSPHAEGLASTSPSGTSVQTNRRVLRKTQSFDTAGSEPVSQYGTCQRTLSEPARRGNTGVFIKGLEVSSTEVIDRPYSPRLPPIHGWSSIDAHRSGSPAPETRSKGSKLRHIVDEMVTTEREYVRSLRYIIDNYFPEMERAVLPQDLRGKRSVIFGNLEKLVDFHSQYFLKELESCCNHPLRVSHCFLRHQDQFSLYALYSKNKPKSDTLLASHGNNFFKHKQMELGDKMDLASYLLKPIQRMSKYALLLKDLIKEVSEVQEQELTYLRAAAEMVKFQLRHGNDLLAMDAIRDCDVNLKEQGQLVRQDEFTISYGRKKCQRHVFLFEDLVLFSKPKRIEGGLDVYIYKHSFKTADVGMTETSGENSLRFEIWFRRRTSKNQTYILQSSTADIKHAWTCDIARILWQQATRNKEIRMQEMVSMGVGNKPFLDIKPSDAAINDRAIDYIMKGRGARTRASIAVSLFDHSNPFKRAAVNTPVSGTPVSGGPSSSTLLGPLNLHMYSSQSLLAGERPLIGPCIEEDELEHETSSQPSMTTESSGSSSHCLSGSGSSGSDSGCVSSHLPEALSEEPSSPCDSSCYSSITSPTQEKPCFNSQYISAGDAQVIGPSTIV

Target ORF information:

RefSeq Version	XM_021471996.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471996.1

ORF Insert Sequence:

ATGACCGCCG CTGCTCCGTG CTCCGGTCAT GGGACCACCG GCGTCTTAGC CGAGCAGGAA 
AATGGATATA GCCATGGAGA GGCATATGAA TGGGCTCTAG AGGGCATGCG TCATTTGGCG 
TGTGTCAGTA TGGAGGATTG TGGCATGTCT GAAAAGTGTC AGAGCGTGAT CACGTGTTTG 
GAGACTTACC GCAGTCAGCA CCCCCCGATC CCTGATGCAC ACTTCCAGGA GATGAAGGAC 
CTCGCTGGGG AACTGAAAAG CGAACAGGGC CTCAAGCAGT GGAAGTTTGC CTGGTCCAAG 
TGTCAGGAGA CCAAGCAGAT GTTTGAAAAG AAGCTGGAGA TGGCCCTGCG TTCCCGCCGT 
GACAGCGACT CCAAGTCAGT AAGGAGTGAC TCTTCTCGTC GTAACTCAGA TGTTAGCACT 
AAACACCAAG AGCGTAGCAG TAGCATCTCC TTCTCATGTC GGAAAGCGTT CGGCGGAGCC 
TGGGGTCGAG ATAGACTTTC CTCACATTCT AGCACATCCT CAACTCCCAG TAGCCCTTCA 
GGCATTCGTC TGGATGCCCG TGACACTGTC CGAACTCCAA CAGGAAGTCC ATACAGCATT 
GAGCACAGAA TCCCCCACAG CACCCCTGTC AGATCCCACA GGCTAACACG TAGCATCTCC 
ACAGATGAGT CTCCTCAGAG CCCACATGCA GAGGGGCTAG CGTCCACAAG CCCAAGCGGC 
ACTTCTGTTC AAACAAACCG CAGGGTTTTG CGGAAGACCC AGAGCTTCGA TACAGCTGGC 
AGTGAGCCTG TGTCACAGTA CGGGACCTGT CAGAGGACTT TGAGTGAGCC TGCACGCAGA 
GGAAACACAG GGGTGTTCAT TAAGGGTCTG GAGGTGAGCA GCACTGAAGT GATTGATAGG 
CCGTACAGTC CACGACTACC ACCCATCCAT GGATGGTCTT CAATTGATGC ACACCGCAGC 
GGGAGTCCTG CTCCAGAAAC GCGCAGCAAG GGCAGCAAGT TACGACACAT TGTAGATGAG 
ATGGTGACAA CAGAGCGGGA ATATGTGCGT TCCCTGCGGT ATATCATTGA CAACTATTTC 
CCTGAGATGG AGCGCGCTGT CCTGCCGCAG GATCTGCGGG GAAAGCGGAG CGTCATATTT 
GGAAATCTGG AGAAACTGGT GGACTTTCAT AGTCAGTATT TCCTAAAAGA GCTGGAGAGC 
TGCTGTAATC ATCCTCTACG CGTCAGCCAC TGTTTCCTAC GACATCAAGA CCAGTTTAGC 
CTCTATGCAT TATACAGCAA GAACAAGCCA AAATCAGACA CACTCCTGGC CAGCCATGGA 
AACAACTTCT TCAAGCATAA GCAGATGGAG CTGGGAGATA AGATGGACCT GGCATCGTAC 
CTGCTGAAGC CCATCCAGCG CATGAGTAAA TACGCTCTTC TGCTGAAAGA CCTGATCAAA 
GAAGTGAGCG AGGTCCAGGA GCAGGAACTT ACATACCTGC GAGCCGCAGC AGAGATGGTC 
AAGTTTCAGC TCCGCCATGG CAATGATCTA CTCGCCATGG ACGCTATCAG AGATTGTGAT 
GTGAACCTAA AAGAGCAAGG ACAGTTGGTT CGTCAAGATG AGTTCACGAT TTCATATGGC 
AGAAAGAAGT GCCAGAGACA CGTGTTCCTA TTTGAGGACC TAGTGCTCTT CAGCAAACCC 
AAGCGCATCG AGGGTGGACT AGATGTTTAC ATATACAAGC ATTCTTTTAA GACGGCTGAT 
GTGGGTATGA CAGAGACGTC AGGTGAGAAC AGTCTGAGGT TTGAAATTTG GTTTCGGAGG 
AGAACTTCAA AGAACCAGAC CTACATTCTC CAGTCCAGCA CTGCTGACAT CAAACATGCC 
TGGACTTGCG ACATCGCACG AATACTGTGG CAGCAGGCCA CGCGAAACAA AGAAATTCGA 
ATGCAGGAGA TGGTCTCAAT GGGTGTGGGC AATAAACCCT TCCTGGACAT TAAACCCAGT 
GATGCTGCAA TCAACGACAG AGCCATCGAC TACATTATGA AGGGCAGAGG AGCTAGAACG 
AGAGCCTCCA TTGCTGTTTC TCTGTTTGAC CACTCAAACC CATTCAAAAG AGCTGCAGTA 
AACACCCCCG TCAGCGGGAC CCCGGTGTCT GGCGGTCCTT CCTCATCCAC CCTGCTGGGG 
CCCTTGAATC TGCACATGTA CAGCAGCCAA AGTCTGTTGG CGGGGGAGAG ACCTCTGATT 
GGCCCCTGCA TCGAGGAGGA CGAGCTGGAG CATGAGACCA GTAGTCAGCC CTCTATGACT 
ACAGAGAGTT CGGGTTCGTC GTCTCACTGT CTGTCTGGCA GTGGCTCTAG CGGATCAGAC 
AGCGGCTGTG TGTCCAGTCA CCTTCCTGAG GCTTTGTCTG AGGAGCCCAG CTCACCTTGT 
GACTCCTCGT GTTATTCCTC CATCACCTCT CCCACGCAGG AGAAGCCCTG CTTTAACAGC 
CAGTATATCT CAGCGGGGGA TGCTCAGGTG ATCGGACCCT CTACTATAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg4 ( XM_021471994.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327669.1 Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa144922
Clone ID Related Accession (Same CDS sequence)	XM_021471994.1
Accession Version	XM_021471994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family G member 4B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394699.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGAT GCATTCATAC TGTACTGCTG CTGGCCGACA GAGATCTGGC TCTACGCATG 
GAGAAAACCT CTTCTATACA GGTGGAGCTG CTGACATCAC TCAAGTCTCT CTATAAACAT 
GTGGACGTTT CTCAGCTTCC CACTGAGTTT GAAGGCACTT TCCCTTATGC ACACAGCAGC 
TGGGTCAGCT TTAGAATGAG GTTGGAGCAG TTGACCAGTC ATTGTGAAGA TGCTGTGAGC 
CTGCTTCAGA ACACCATAAG CAAACTGGAG TCCACAGTGC TGCCCCCTAC AGCAGAGGAG 
GCTCAACTCT TACTGTGTAA GTACAGAGAG CTGATGAGGA CGGTTCTGGA GGACAGCAGG 
CTGGTGCGAC TACAGTTGGA GGGAGGAGCA GCTCTGTCCC GTTTACGAAA AGAAGAGACC 
AGCGTTAGTC TCACAGAGGA CTACAGAGAT GCCATTGAAA GTGCTGGCCG TTTGTATAAC 
CAAGTGGACG AGCTGGTTCA CAGACTGGTG ATGCTGTCCA ATAAATGCAC ACAGGAACTG 
GAATTCATCA TGGAGTTTAA GAGCCTGGAG GAGGGATTCA AAGAGGTTAG TCAGTGGATA 
GAAGAGGTGG GAGAGTCTCG TCTGCAGACG CTGAGCGAGC TGGAGGATTC CTTGGAGCAG 
TTACATCATA AGCAGAGCAT CTTCAGAGAA TTTTACACAG CTGCATATGA ACACTGTAAG 
AGTGGTGAAG CTCTCCTCAA GCGTTTAGAG AAGTGGGAAG ACGTCTCCTC TGCAGAGCTG 
CAAGTGTATG AGGTGAAAGT ACGTTCATTC TGGGTGCATC TGCGGGATTT CTCTCAGCGA 
GTGGAGGACA CCAAGGATAA AATAGACAAG ACTGTTCAAC TCTATGAGTT CTTTGATAAG 
GCATATGAAT GGGCTCTAGA GGGCATGCGT CATTTGGCGT GTGTCAGTAT GGAGGATTGT 
GGCATGTCTG AAAAGTGTCA GAGCGTGATC ACGTGTTTGG AGACTTACCG CAGTCAGCAC 
CCCCCGATCC CTGATGCACA CTTCCAGGAG ATGAAGGACC TCGCTGGGGA ACTGAAAAGC 
GAACAGGGCC TCAAGCAGTG GAAGTTTGCC TGGTCCAAGT GTCAGGAGAC CAAGCAGATG 
TTTGAAAAGA AGCTGGAGAT GGCCCTGCGT TCCCGCCGTG ACAGCGACTC CAAGTCAGTA 
AGGAGTGACT CTTCTCGTCG TAACTCAGAT GTTAGCACTA AACACCAAGA GCGTAGCAGT 
AGCATCTCCT TCTCATGTCG GAAAGCGTTC GGCGGAGCCT GGGGTCGAGA TAGACTTTCC 
TCACATTCTA GCACATCCTC AACTCCCAGT AGCCCTTCAG GCATTCGTCT GGATGCCCGT 
GACACTGTCC GAACTCCAAC AGGAAGTCCA TACAGCATTG AGCACAGAAT CCCCCACAGC 
ACCCCTGTCA GATCCCACAG GCTAACACGT AGCATCTCCA CAGATGAGTC TCCTCAGAGC 
CCACATGCAG AGGGGCTAGC GTCCACAAGC CCAAGCGGCA CTTCTGTTCA AACAAACCGC 
AGGGTTTTGC GGAAGACCCA GAGCTTCGAT ACAGCTGGCA GTGAGCCTGT GTCACAGTAC 
GGGACCTGTC AGAGGACTTT GAGTGAGCCT GCACGCAGAG GAAACACAGG GGTGTTCATT 
AAGGGTCTGG AGGTGAGCAG CACTGAAGTG ATTGATAGGC CGTACAGTCC ACGACTACCA 
CCCATCCATG GATGGTCTTC AATTGATGCA CACCGCAGCG GGAGTCCTGC TCCAGAAACG 
CGCAGCAAGG GCAGCAAGTT ACGACACATT GTAGATGAGA TGGTGACAAC AGAGCGGGAA 
TATGTGCGTT CCCTGCGGTA TATCATTGAC AACTATTTCC CTGAGATGGA GCGCGCTGTC 
CTGCCGCAGG ATCTGCGGGG AAAGCGGAGC GTCATATTTG GAAATCTGGA GAAACTGGTG 
GACTTTCATA GTCAGTATTT CCTAAAAGAG CTGGAGAGCT GCTGTAATCA TCCTCTACGC 
GTCAGCCACT GTTTCCTACG ACATCAAGAC CAGTTTAGCC TCTATGCATT ATACAGCAAG 
AACAAGCCAA AATCAGACAC ACTCCTGGCC AGCCATGGAA ACAACTTCTT CAAGCATAAG 
CAGATGGAGC TGGGAGATAA GATGGACCTG GCATCGTACC TGCTGAAGCC CATCCAGCGC 
ATGAGTAAAT ACGCTCTTCT GCTGAAAGAC CTGATCAAAG AAGTGAGCGA GGTCCAGGAG 
CAGGAACTTA CATACCTGCG AGCCGCAGCA GAGATGGTCA AGTTTCAGCT CCGCCATGGC 
AATGATCTAC TCGCCATGGA CGCTATCAGA GATTGTGATG TGAACCTAAA AGAGCAAGGA 
CAGTTGGTTC GTCAAGATGA GTTCACGATT TCATATGGCA GAAAGAAGTG CCAGAGACAC 
GTGTTCCTAT TTGAGGACCT AGTGCTCTTC AGCAAACCCA AGCGCATCGA GGGTGGACTA 
GATGTTTACA TATACAAGCA TTCTTTTAAG ACGGCTGATG TGGGTATGAC AGAGACGTCA 
GGTGAGAACA GTCTGAGGTT TGAAATTTGG TTTCGGAGGA GAACTTCAAA GAACCAGACC 
TACATTCTCC AGTCCAGCAC TGCTGACATC AAACATGCCT GGACTTGCGA CATCGCACGA 
ATACTGTGGC AGCAGGCCAC GCGAAACAAA GAAATTCGAA TGCAGGAGAT GGTCTCAATG 
GGTGTGGGCA ATAAACCCTT CCTGGACATT AAACCCAGTG ATGCTGCAAT CAACGACAGA 
GCCATCGACT ACATTATGAA GGGCAGAGGA GCTAGAACGA GAGCCTCCAT TGCTGTTTCT 
CTGTTTGACC ACTCAAACCC ATTCAAAAGA GCTGCAGTAA ACACCCCCGT CAGCGGGACC 
CCGGTGTCTG GCGGTCCTTC CTCATCCACC CTGCTGGGGC CCTTGAATCT GCACATGTAC 
AGCAGCCAAA GTCTGTTGGC GGGGGAGAGA CCTCTGATTG GCCCCTGCAT CGAGGAGGAC 
GAGCTGGAGC ATGAGACCAG TAGTCAGCCC TCTATGACTA CAGAGAGTTC GGGTTCGTCG 
TCTCACTGTC TGTCTGGCAG TGGCTCTAGC GGATCAGACA GCGGCTGTGT GTCCAGTCAC 
CTTCCTGAGG CTTTGTCTGA GGAGCCCAGC TCACCTTGTG ACTCCTCGTG TTATTCCTCC 
ATCACCTCTC CCACGCAGGA GAAGCCCTGC TTTAACAGCC AGTATATCTC AGCGGGGGAT 
GCTCAGGTGA TCGGACCCTC TACTATAGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327669.1
CDS	29..3361
Translation	MPGCIHTVLLLADRDLALRMEKTSSIQVELLTSLKSLYKHVDVSQLPTEFEGTFPYAHSSWVSFRMRLEQLTSHCEDAVSLLQNTISKLESTVLPPTAEEAQLLLCKYRELMRTVLEDSRLVRLQLEGGAALSRLRKEETSVSLTEDYRDAIESAGRLYNQVDELVHRLVMLSNKCTQELEFIMEFKSLEEGFKEVSQWIEEVGESRLQTLSELEDSLEQLHHKQSIFREFYTAAYEHCKSGEALLKRLEKWEDVSSAELQVYEVKVRSFWVHLRDFSQRVEDTKDKIDKTVQLYEFFDKAYEWALEGMRHLACVSMEDCGMSEKCQSVITCLETYRSQHPPIPDAHFQEMKDLAGELKSEQGLKQWKFAWSKCQETKQMFEKKLEMALRSRRDSDSKSVRSDSSRRNSDVSTKHQERSSSISFSCRKAFGGAWGRDRLSSHSSTSSTPSSPSGIRLDARDTVRTPTGSPYSIEHRIPHSTPVRSHRLTRSISTDESPQSPHAEGLASTSPSGTSVQTNRRVLRKTQSFDTAGSEPVSQYGTCQRTLSEPARRGNTGVFIKGLEVSSTEVIDRPYSPRLPPIHGWSSIDAHRSGSPAPETRSKGSKLRHIVDEMVTTEREYVRSLRYIIDNYFPEMERAVLPQDLRGKRSVIFGNLEKLVDFHSQYFLKELESCCNHPLRVSHCFLRHQDQFSLYALYSKNKPKSDTLLASHGNNFFKHKQMELGDKMDLASYLLKPIQRMSKYALLLKDLIKEVSEVQEQELTYLRAAAEMVKFQLRHGNDLLAMDAIRDCDVNLKEQGQLVRQDEFTISYGRKKCQRHVFLFEDLVLFSKPKRIEGGLDVYIYKHSFKTADVGMTETSGENSLRFEIWFRRRTSKNQTYILQSSTADIKHAWTCDIARILWQQATRNKEIRMQEMVSMGVGNKPFLDIKPSDAAINDRAIDYIMKGRGARTRASIAVSLFDHSNPFKRAAVNTPVSGTPVSGGPSSSTLLGPLNLHMYSSQSLLAGERPLIGPCIEEDELEHETSSQPSMTTESSGSSSHCLSGSGSSGSDSGCVSSHLPEALSEEPSSPCDSSCYSSITSPTQEKPCFNSQYISAGDAQVIGPSTIV

Target ORF information:

RefSeq Version	XM_021471994.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471994.1

ORF Insert Sequence:

ATGCCGGGAT GCATTCATAC TGTACTGCTG CTGGCCGACA GAGATCTGGC TCTACGCATG 
GAGAAAACCT CTTCTATACA GGTGGAGCTG CTGACATCAC TCAAGTCTCT CTATAAACAT 
GTGGACGTTT CTCAGCTTCC CACTGAGTTT GAAGGCACTT TCCCTTATGC ACACAGCAGC 
TGGGTCAGCT TTAGAATGAG GTTGGAGCAG TTGACCAGTC ATTGTGAAGA TGCTGTGAGC 
CTGCTTCAGA ACACCATAAG CAAACTGGAG TCCACAGTGC TGCCCCCTAC AGCAGAGGAG 
GCTCAACTCT TACTGTGTAA GTACAGAGAG CTGATGAGGA CGGTTCTGGA GGACAGCAGG 
CTGGTGCGAC TACAGTTGGA GGGAGGAGCA GCTCTGTCCC GTTTACGAAA AGAAGAGACC 
AGCGTTAGTC TCACAGAGGA CTACAGAGAT GCCATTGAAA GTGCTGGCCG TTTGTATAAC 
CAAGTGGACG AGCTGGTTCA CAGACTGGTG ATGCTGTCCA ATAAATGCAC ACAGGAACTG 
GAATTCATCA TGGAGTTTAA GAGCCTGGAG GAGGGATTCA AAGAGGTTAG TCAGTGGATA 
GAAGAGGTGG GAGAGTCTCG TCTGCAGACG CTGAGCGAGC TGGAGGATTC CTTGGAGCAG 
TTACATCATA AGCAGAGCAT CTTCAGAGAA TTTTACACAG CTGCATATGA ACACTGTAAG 
AGTGGTGAAG CTCTCCTCAA GCGTTTAGAG AAGTGGGAAG ACGTCTCCTC TGCAGAGCTG 
CAAGTGTATG AGGTGAAAGT ACGTTCATTC TGGGTGCATC TGCGGGATTT CTCTCAGCGA 
GTGGAGGACA CCAAGGATAA AATAGACAAG ACTGTTCAAC TCTATGAGTT CTTTGATAAG 
GCATATGAAT GGGCTCTAGA GGGCATGCGT CATTTGGCGT GTGTCAGTAT GGAGGATTGT 
GGCATGTCTG AAAAGTGTCA GAGCGTGATC ACGTGTTTGG AGACTTACCG CAGTCAGCAC 
CCCCCGATCC CTGATGCACA CTTCCAGGAG ATGAAGGACC TCGCTGGGGA ACTGAAAAGC 
GAACAGGGCC TCAAGCAGTG GAAGTTTGCC TGGTCCAAGT GTCAGGAGAC CAAGCAGATG 
TTTGAAAAGA AGCTGGAGAT GGCCCTGCGT TCCCGCCGTG ACAGCGACTC CAAGTCAGTA 
AGGAGTGACT CTTCTCGTCG TAACTCAGAT GTTAGCACTA AACACCAAGA GCGTAGCAGT 
AGCATCTCCT TCTCATGTCG GAAAGCGTTC GGCGGAGCCT GGGGTCGAGA TAGACTTTCC 
TCACATTCTA GCACATCCTC AACTCCCAGT AGCCCTTCAG GCATTCGTCT GGATGCCCGT 
GACACTGTCC GAACTCCAAC AGGAAGTCCA TACAGCATTG AGCACAGAAT CCCCCACAGC 
ACCCCTGTCA GATCCCACAG GCTAACACGT AGCATCTCCA CAGATGAGTC TCCTCAGAGC 
CCACATGCAG AGGGGCTAGC GTCCACAAGC CCAAGCGGCA CTTCTGTTCA AACAAACCGC 
AGGGTTTTGC GGAAGACCCA GAGCTTCGAT ACAGCTGGCA GTGAGCCTGT GTCACAGTAC 
GGGACCTGTC AGAGGACTTT GAGTGAGCCT GCACGCAGAG GAAACACAGG GGTGTTCATT 
AAGGGTCTGG AGGTGAGCAG CACTGAAGTG ATTGATAGGC CGTACAGTCC ACGACTACCA 
CCCATCCATG GATGGTCTTC AATTGATGCA CACCGCAGCG GGAGTCCTGC TCCAGAAACG 
CGCAGCAAGG GCAGCAAGTT ACGACACATT GTAGATGAGA TGGTGACAAC AGAGCGGGAA 
TATGTGCGTT CCCTGCGGTA TATCATTGAC AACTATTTCC CTGAGATGGA GCGCGCTGTC 
CTGCCGCAGG ATCTGCGGGG AAAGCGGAGC GTCATATTTG GAAATCTGGA GAAACTGGTG 
GACTTTCATA GTCAGTATTT CCTAAAAGAG CTGGAGAGCT GCTGTAATCA TCCTCTACGC 
GTCAGCCACT GTTTCCTACG ACATCAAGAC CAGTTTAGCC TCTATGCATT ATACAGCAAG 
AACAAGCCAA AATCAGACAC ACTCCTGGCC AGCCATGGAA ACAACTTCTT CAAGCATAAG 
CAGATGGAGC TGGGAGATAA GATGGACCTG GCATCGTACC TGCTGAAGCC CATCCAGCGC 
ATGAGTAAAT ACGCTCTTCT GCTGAAAGAC CTGATCAAAG AAGTGAGCGA GGTCCAGGAG 
CAGGAACTTA CATACCTGCG AGCCGCAGCA GAGATGGTCA AGTTTCAGCT CCGCCATGGC 
AATGATCTAC TCGCCATGGA CGCTATCAGA GATTGTGATG TGAACCTAAA AGAGCAAGGA 
CAGTTGGTTC GTCAAGATGA GTTCACGATT TCATATGGCA GAAAGAAGTG CCAGAGACAC 
GTGTTCCTAT TTGAGGACCT AGTGCTCTTC AGCAAACCCA AGCGCATCGA GGGTGGACTA 
GATGTTTACA TATACAAGCA TTCTTTTAAG ACGGCTGATG TGGGTATGAC AGAGACGTCA 
GGTGAGAACA GTCTGAGGTT TGAAATTTGG TTTCGGAGGA GAACTTCAAA GAACCAGACC 
TACATTCTCC AGTCCAGCAC TGCTGACATC AAACATGCCT GGACTTGCGA CATCGCACGA 
ATACTGTGGC AGCAGGCCAC GCGAAACAAA GAAATTCGAA TGCAGGAGAT GGTCTCAATG 
GGTGTGGGCA ATAAACCCTT CCTGGACATT AAACCCAGTG ATGCTGCAAT CAACGACAGA 
GCCATCGACT ACATTATGAA GGGCAGAGGA GCTAGAACGA GAGCCTCCAT TGCTGTTTCT 
CTGTTTGACC ACTCAAACCC ATTCAAAAGA GCTGCAGTAA ACACCCCCGT CAGCGGGACC 
CCGGTGTCTG GCGGTCCTTC CTCATCCACC CTGCTGGGGC CCTTGAATCT GCACATGTAC 
AGCAGCCAAA GTCTGTTGGC GGGGGAGAGA CCTCTGATTG GCCCCTGCAT CGAGGAGGAC 
GAGCTGGAGC ATGAGACCAG TAGTCAGCCC TCTATGACTA CAGAGAGTTC GGGTTCGTCG 
TCTCACTGTC TGTCTGGCAG TGGCTCTAGC GGATCAGACA GCGGCTGTGT GTCCAGTCAC 
CTTCCTGAGG CTTTGTCTGA GGAGCCCAGC TCACCTTGTG ACTCCTCGTG TTATTCCTCC 
ATCACCTCTC CCACGCAGGA GAAGCCCTGC TTTAACAGCC AGTATATCTC AGCGGGGGAT 
GCTCAGGTGA TCGGACCCTC TACTATAGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg4 ( XM_021471995.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327670.1 Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa144923
Clone ID Related Accession (Same CDS sequence)	XM_021471995.1
Accession Version	XM_021471995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family G member 4B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394699.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGAT GCATTCATAC TGTACTGCTG CTGGCCGACA GAGATCTGGC TCTACGCATG 
GAGAAAACCT CTTCTATACA GGTGGAGCTG CTGACATCAC TCAAGTCTCT CTATAAACAT 
GTGGACGTTT CTCAGCTTCC CACTGAGTTT GAAGGCACTT TCCCTTATGC ACACAGCAGC 
TGGGTCAGCT TTAGAATGAG GTTGGAGCAG TTGACCAGTC ATTGTGAAGA TGCTGTGAGC 
CTGCTTCAGA ACACCATAAG CAAACTGGAG TCCACAGTGC TGCCCCCTAC AGCAGAGGAG 
GCTCAACTCT TACTGTGTAA GTACAGAGAG CTGATGAGGA CGGTTCTGGA GGACAGCAGG 
CTGGTGCGAC TACAGTTGGA GGGAGGAGCA GCTCTGTCCC GTTTACGAAA AGAAGAGACC 
AGCGTTAGTC TCACAGAGGA CTACAGAGAT GCCATTGAAA GTGCTGGCCG TTTGTATAAC 
CAAGTGGACG AGCTGGTTCA CAGACTGGTG ATGCTGTCCA ATAAATGCAC ACAGGAACTG 
GAATTCATCA TGGAGTTTAA GAGCCTGGAG GAGGGATTCA AAGAGGTTAG TCAGTGGATA 
GAAGAGGTGG GAGAGTCTCG TCTGCAGACG CTGAGCGAGC TGGAGGATTC CTTGGAGCAG 
TTACATCATA AGCAGAGCAT CTTCAGAGAA TTTTACACAG CTGCATATGA ACACTGTAAG 
AGTGGTGAAG CTCTCCTCAA GCGTTTAGAG AAGTGGGAAG ACGTCTCCTC TGCAGAGCTG 
CAAGTGTATG AGGTGAAAGT ACGTTCATTC TGGGTGCATC TGCGGGATTT CTCTCAGCGA 
GTGGAGGACA CCAAGGATAA AATAGACAAG ACTGTTCAAC TCTATGAGTT CTTTGATAAG 
GCATATGAAT GGGCTCTAGA GGGCATGCGT CATTTGGCGT GTGTCAGTAT GGAGGATTGT 
GGCATGTCTG AAAAGTGTCA GAGCGTGATC ACGTGTTTGG AGACTTACCG CAGTCAGCAC 
CCCCCGATCC CTGATGCACA CTTCCAGGAG ATGAAGGACC TCGCTGGGGA ACTGAAAAGC 
GAACAGGGCC TCAAGCAGTG GAAGTTTGCC TGGTCCAAGT GTCAGGAGAC CAAGCAGATG 
TTTGAAAAGA AGCTGGAGAT GGCCCTGCGT TCCCGCCGTG ACAGCGACTC CAAGTCAGTA 
AGGAGTGACT CTTCTCGTCG TAACTCAGAT GTTAGCACTA AACACCAAGA GCGTAGCAGT 
AGCATCTCCT TCTCATGTCG GAAAGCGTTC GGCGGAGCCT GGGGTCGAGA TAGACTTTCC 
TCACATTCTA GCACATCCTC AACTCCCAGT AGCCCTTCAG GCATTCGTCT GGATGCCCGT 
GACACTGTCC GAACTCCAAC AGGAAGTCCA TACAGCATTG AGCACAGAAT CCCCCACAGC 
ACCCCTGTCA GATCCCACAG GCTAACACGT AGCATCTCCA CAGATGAGTC TCCTCAGAGC 
CCACATGCAG AGGGGCTAGC GTCCACAAGC CCAAGCGGCA CTTCTGTTCA AACAAACCGC 
AGGGTTTTGC GGAAGACCCA GAGCTTCGAT ACAGCTGGCA GTGAGCCTGT GTCACAGTAC 
GGGACCTGTC AGAGGACTTT GAGTGAGCCT GCACGCAGAG GAAACACAGG GGTGTTCATT 
AAGGGTCTGG AGGTGAGCAG CACTGAAGTG ATTGATAGGC CGTACAGTCC ACGACTACCA 
CCCATCCATG GATGGTCTTC AATTGATGCA CACCGCAGCG GGAGTCCTGC TCCAGAAACG 
CGCAGCAAGG GCAGCAAGTT ACGACACATT GTAGATGAGA TGGTGACAAC AGAGCGGGAA 
TATGTGCGTT CCCTGCGGTA TATCATTGAC AACTATTTCC CTGAGATGGA GCGCGCTGTC 
CTGCCGCAGG ATCTGCGGGG AAAGCGGAGC GTCATATTTG GAAATCTGGA GAAACTGGTG 
GACTTTCATA GTCAGTATTT CCTAAAAGAG CTGGAGAGCT GCTGTAATCA TCCTCTACGC 
GTCAGCCACT GTTTCCTACG ACATCAAGAC CAGTTTAGCC TCTATGCATT ATACAGCAAG 
AACAAGCCAA AATCAGACAC ACTCCTGGCC AGCCATGGAA ACAACTTCTT CAAGCATAAG 
CAGATGGAGC TGGGAGATAA GATGGACCTG GCATCGTACC TGCTGAAGCC CATCCAGCGC 
ATGAGTAAAT ACGCTCTTCT GCTGAAAGAC CTGATCAAAG AAGTGAGCGA GGTCCAGGAG 
CAGGAACTTA CATACCTGCG AGCCGCAGCA GAGATGGTCA AGTTTCAGCT CCGCCATGGC 
AATGATCTAC TCGCCATGGA CGCTATCAGA GATTGTGATG TGAACCTAAA AGAGCAAGGA 
CAGTTGGTTC GTCAAGATGA GTTCACGATT TCATATGGCA GAAAGAAGTG CCAGAGACAC 
GTGTTCCTAT TTGAGGACCT AGTGCTCTTC AGCAAACCCA AGCGCATCGA GGGTGGACTA 
GATGTTTACA TATACAAGCA TTCTTTTAAG ACGGCTGATG TGGGTATGAC AGAGACGTCA 
GGTGAGAACA GTCTGAGGTT TGAAATTTGG TTTCGGAGGA GAACTTCAAA GAACCAGACC 
TACATTCTCC AGTCCAGCAC TGCTGACATC AAACATGCCT GGACTTGCGA CATCGCACGA 
ATACTGTGGC AGCAGGCCAC GCGAAACAAA GAAATTCGAA TGCAGGAGAT GGTCTCAATG 
GGTGTGGGCA ATAAACCCTT CCTGGACATT AAACCCAGTG ATGCTGCAAT CAACGACAGA 
GCCATCGACT ACATTATGAA GGGCAGAGGA GCTAGAACGA GAGCCTCCAT TGCTGTTTCT 
CTGTTTGACC ACTCAAACCC ATTCAAAAGA GCTGCAGTAA ACACCCCCGT CAGCGGGACC 
CCGGTGTCTG GCGGTCCTTC CTCATCCACC CTGCTGGGGC CCTTGAATCT GCACATGTAC 
AGCAGCCAAA GTCTGTTGGC GGGGGAGAGA CCTCTGATTG GCCCCTGCAT CGAGGAGGAC 
GAGCTGGAGC ATGAGACCAG TAGTCAGCCC TCTATGACTA CAGAGAGTTC GGGTTCGTCG 
TCTCACTGTC TGTCTGGCAG TGGCTCTAGC GGATCAGACA GCGGCTGTGT GTCCAGTCAC 
CTTCCTGAGG CTTTGTCTGA GGAGCCCAGC TCACCTTGTG ACTCCTCGTG TTATTCCTCC 
ATCACCTCTC CCACGCAGGA GAAGCCCTGC TTTAACAGCC AGTATATCTC AGCGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327670.1
CDS	29..3328
Translation	MPGCIHTVLLLADRDLALRMEKTSSIQVELLTSLKSLYKHVDVSQLPTEFEGTFPYAHSSWVSFRMRLEQLTSHCEDAVSLLQNTISKLESTVLPPTAEEAQLLLCKYRELMRTVLEDSRLVRLQLEGGAALSRLRKEETSVSLTEDYRDAIESAGRLYNQVDELVHRLVMLSNKCTQELEFIMEFKSLEEGFKEVSQWIEEVGESRLQTLSELEDSLEQLHHKQSIFREFYTAAYEHCKSGEALLKRLEKWEDVSSAELQVYEVKVRSFWVHLRDFSQRVEDTKDKIDKTVQLYEFFDKAYEWALEGMRHLACVSMEDCGMSEKCQSVITCLETYRSQHPPIPDAHFQEMKDLAGELKSEQGLKQWKFAWSKCQETKQMFEKKLEMALRSRRDSDSKSVRSDSSRRNSDVSTKHQERSSSISFSCRKAFGGAWGRDRLSSHSSTSSTPSSPSGIRLDARDTVRTPTGSPYSIEHRIPHSTPVRSHRLTRSISTDESPQSPHAEGLASTSPSGTSVQTNRRVLRKTQSFDTAGSEPVSQYGTCQRTLSEPARRGNTGVFIKGLEVSSTEVIDRPYSPRLPPIHGWSSIDAHRSGSPAPETRSKGSKLRHIVDEMVTTEREYVRSLRYIIDNYFPEMERAVLPQDLRGKRSVIFGNLEKLVDFHSQYFLKELESCCNHPLRVSHCFLRHQDQFSLYALYSKNKPKSDTLLASHGNNFFKHKQMELGDKMDLASYLLKPIQRMSKYALLLKDLIKEVSEVQEQELTYLRAAAEMVKFQLRHGNDLLAMDAIRDCDVNLKEQGQLVRQDEFTISYGRKKCQRHVFLFEDLVLFSKPKRIEGGLDVYIYKHSFKTADVGMTETSGENSLRFEIWFRRRTSKNQTYILQSSTADIKHAWTCDIARILWQQATRNKEIRMQEMVSMGVGNKPFLDIKPSDAAINDRAIDYIMKGRGARTRASIAVSLFDHSNPFKRAAVNTPVSGTPVSGGPSSSTLLGPLNLHMYSSQSLLAGERPLIGPCIEEDELEHETSSQPSMTTESSGSSSHCLSGSGSSGSDSGCVSSHLPEALSEEPSSPCDSSCYSSITSPTQEKPCFNSQYISAV

Target ORF information:

RefSeq Version	XM_021471995.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471995.1

ORF Insert Sequence:

ATGCCGGGAT GCATTCATAC TGTACTGCTG CTGGCCGACA GAGATCTGGC TCTACGCATG 
GAGAAAACCT CTTCTATACA GGTGGAGCTG CTGACATCAC TCAAGTCTCT CTATAAACAT 
GTGGACGTTT CTCAGCTTCC CACTGAGTTT GAAGGCACTT TCCCTTATGC ACACAGCAGC 
TGGGTCAGCT TTAGAATGAG GTTGGAGCAG TTGACCAGTC ATTGTGAAGA TGCTGTGAGC 
CTGCTTCAGA ACACCATAAG CAAACTGGAG TCCACAGTGC TGCCCCCTAC AGCAGAGGAG 
GCTCAACTCT TACTGTGTAA GTACAGAGAG CTGATGAGGA CGGTTCTGGA GGACAGCAGG 
CTGGTGCGAC TACAGTTGGA GGGAGGAGCA GCTCTGTCCC GTTTACGAAA AGAAGAGACC 
AGCGTTAGTC TCACAGAGGA CTACAGAGAT GCCATTGAAA GTGCTGGCCG TTTGTATAAC 
CAAGTGGACG AGCTGGTTCA CAGACTGGTG ATGCTGTCCA ATAAATGCAC ACAGGAACTG 
GAATTCATCA TGGAGTTTAA GAGCCTGGAG GAGGGATTCA AAGAGGTTAG TCAGTGGATA 
GAAGAGGTGG GAGAGTCTCG TCTGCAGACG CTGAGCGAGC TGGAGGATTC CTTGGAGCAG 
TTACATCATA AGCAGAGCAT CTTCAGAGAA TTTTACACAG CTGCATATGA ACACTGTAAG 
AGTGGTGAAG CTCTCCTCAA GCGTTTAGAG AAGTGGGAAG ACGTCTCCTC TGCAGAGCTG 
CAAGTGTATG AGGTGAAAGT ACGTTCATTC TGGGTGCATC TGCGGGATTT CTCTCAGCGA 
GTGGAGGACA CCAAGGATAA AATAGACAAG ACTGTTCAAC TCTATGAGTT CTTTGATAAG 
GCATATGAAT GGGCTCTAGA GGGCATGCGT CATTTGGCGT GTGTCAGTAT GGAGGATTGT 
GGCATGTCTG AAAAGTGTCA GAGCGTGATC ACGTGTTTGG AGACTTACCG CAGTCAGCAC 
CCCCCGATCC CTGATGCACA CTTCCAGGAG ATGAAGGACC TCGCTGGGGA ACTGAAAAGC 
GAACAGGGCC TCAAGCAGTG GAAGTTTGCC TGGTCCAAGT GTCAGGAGAC CAAGCAGATG 
TTTGAAAAGA AGCTGGAGAT GGCCCTGCGT TCCCGCCGTG ACAGCGACTC CAAGTCAGTA 
AGGAGTGACT CTTCTCGTCG TAACTCAGAT GTTAGCACTA AACACCAAGA GCGTAGCAGT 
AGCATCTCCT TCTCATGTCG GAAAGCGTTC GGCGGAGCCT GGGGTCGAGA TAGACTTTCC 
TCACATTCTA GCACATCCTC AACTCCCAGT AGCCCTTCAG GCATTCGTCT GGATGCCCGT 
GACACTGTCC GAACTCCAAC AGGAAGTCCA TACAGCATTG AGCACAGAAT CCCCCACAGC 
ACCCCTGTCA GATCCCACAG GCTAACACGT AGCATCTCCA CAGATGAGTC TCCTCAGAGC 
CCACATGCAG AGGGGCTAGC GTCCACAAGC CCAAGCGGCA CTTCTGTTCA AACAAACCGC 
AGGGTTTTGC GGAAGACCCA GAGCTTCGAT ACAGCTGGCA GTGAGCCTGT GTCACAGTAC 
GGGACCTGTC AGAGGACTTT GAGTGAGCCT GCACGCAGAG GAAACACAGG GGTGTTCATT 
AAGGGTCTGG AGGTGAGCAG CACTGAAGTG ATTGATAGGC CGTACAGTCC ACGACTACCA 
CCCATCCATG GATGGTCTTC AATTGATGCA CACCGCAGCG GGAGTCCTGC TCCAGAAACG 
CGCAGCAAGG GCAGCAAGTT ACGACACATT GTAGATGAGA TGGTGACAAC AGAGCGGGAA 
TATGTGCGTT CCCTGCGGTA TATCATTGAC AACTATTTCC CTGAGATGGA GCGCGCTGTC 
CTGCCGCAGG ATCTGCGGGG AAAGCGGAGC GTCATATTTG GAAATCTGGA GAAACTGGTG 
GACTTTCATA GTCAGTATTT CCTAAAAGAG CTGGAGAGCT GCTGTAATCA TCCTCTACGC 
GTCAGCCACT GTTTCCTACG ACATCAAGAC CAGTTTAGCC TCTATGCATT ATACAGCAAG 
AACAAGCCAA AATCAGACAC ACTCCTGGCC AGCCATGGAA ACAACTTCTT CAAGCATAAG 
CAGATGGAGC TGGGAGATAA GATGGACCTG GCATCGTACC TGCTGAAGCC CATCCAGCGC 
ATGAGTAAAT ACGCTCTTCT GCTGAAAGAC CTGATCAAAG AAGTGAGCGA GGTCCAGGAG 
CAGGAACTTA CATACCTGCG AGCCGCAGCA GAGATGGTCA AGTTTCAGCT CCGCCATGGC 
AATGATCTAC TCGCCATGGA CGCTATCAGA GATTGTGATG TGAACCTAAA AGAGCAAGGA 
CAGTTGGTTC GTCAAGATGA GTTCACGATT TCATATGGCA GAAAGAAGTG CCAGAGACAC 
GTGTTCCTAT TTGAGGACCT AGTGCTCTTC AGCAAACCCA AGCGCATCGA GGGTGGACTA 
GATGTTTACA TATACAAGCA TTCTTTTAAG ACGGCTGATG TGGGTATGAC AGAGACGTCA 
GGTGAGAACA GTCTGAGGTT TGAAATTTGG TTTCGGAGGA GAACTTCAAA GAACCAGACC 
TACATTCTCC AGTCCAGCAC TGCTGACATC AAACATGCCT GGACTTGCGA CATCGCACGA 
ATACTGTGGC AGCAGGCCAC GCGAAACAAA GAAATTCGAA TGCAGGAGAT GGTCTCAATG 
GGTGTGGGCA ATAAACCCTT CCTGGACATT AAACCCAGTG ATGCTGCAAT CAACGACAGA 
GCCATCGACT ACATTATGAA GGGCAGAGGA GCTAGAACGA GAGCCTCCAT TGCTGTTTCT 
CTGTTTGACC ACTCAAACCC ATTCAAAAGA GCTGCAGTAA ACACCCCCGT CAGCGGGACC 
CCGGTGTCTG GCGGTCCTTC CTCATCCACC CTGCTGGGGC CCTTGAATCT GCACATGTAC 
AGCAGCCAAA GTCTGTTGGC GGGGGAGAGA CCTCTGATTG GCCCCTGCAT CGAGGAGGAC 
GAGCTGGAGC ATGAGACCAG TAGTCAGCCC TCTATGACTA CAGAGAGTTC GGGTTCGTCG 
TCTCACTGTC TGTCTGGCAG TGGCTCTAGC GGATCAGACA GCGGCTGTGT GTCCAGTCAC 
CTTCCTGAGG CTTTGTCTGA GGAGCCCAGC TCACCTTGTG ACTCCTCGTG TTATTCCTCC 
ATCACCTCTC CCACGCAGGA GAAGCCCTGC TTTAACAGCC AGTATATCTC AGCGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg4 ( XM_021477915.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333590.1 Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa143496
Clone ID Related Accession (Same CDS sequence)	XM_021477915.1
Accession Version	XM_021477915.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	puratrophin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTG AATCTCTGGA CAGCTGTATC CAGAGCACAC TGTCCGCTCT CTACCCTCCA 
TTTGAAGCCA CTGCGGCCAC TGTACTGTGC CAGGTGTTAG ATGTGGTGGA GACAACATAC 
CGTGGAGACG GACTGCGATA CATCATCGAC TTTCTGGTGC CCGCTAAACA CATCCTACAG 
AGCATTCAGC AGGATGCATG CTTCCCATAC TGTGGTTTTC TGTTCCGTCA TGAAGGATGG 
CCACTGTGTG TCCATGAGAA GGTCATAATC CAGCTCTCCT CTTTGGACTG GCGTGTATTA 
CGGCCCAGCG ACTTCTACTT GCAGGTGACA CCATCTGCCA AAAGCCCCCC GCGCATCAAA 
GTGAAGTGTC TGGCTGTTAC CGGGCAGCAT GCGGAGGAGC TGGACGTGCC GGAGCTAGCA 
TATACCTCTC TATTCACCAT GGACTGGCTG GACTCCATCA ACCAGGAGAG GAGTGTGGTC 
AGAAGAGCTG CCCTGGAACA CTGTCTTTTA TCAGCGGATA ATGATATTTT TAGGGTACCG 
TGGGAGGATA TTGTTCACCC GGAATTTATC AGCAGACCCT CTAAAGTTGC AGAACAAAGT 
GAAAGTAGAA GGGTTGCCAA AGAAGTTGCA AATGGAGACG GGCGAGACTC TGATGTAGAA 
CAAGACAACA ATCCTGAGGA CATACAATGT AGAATTTTTT ATGACACTTC CACGAACCAT 
TCAGGGGATA TGGATTGTCA GAGCAAGGAA GTTAAGCTCC TCCCTGTTCT TAGTGAGATT 
AATAAAGATA TCAGTGGGAG GAGCTCTAGC AGCACTGCGG ACGATTCAGA GGGCGAATAT 
GTGGAGCTAG CTGATATCTC CTTACCACGC TTTTCCCCAC AAAAGGGCTC TCTTACTCAA 
GCCATTAGCA TGAACTATAG AAATCTACAT AAACCTCAGA CTCCTGTTTC CACTCAGCTT 
AAAACCAAAC CAGATGAGAG TCTTTTGAAG AATGGTGCAT GCTCTCAAAG CATGGTGTGT 
GCCATGATGA TCGAGGAAAG CCTGAGTGAT ACCTACCAAA CAGTGATCCT TACTCCCACT 
GCTCCTGCAG AGGCAGAGCG TCCGCTCCAG CAGGTGGACA TGTCTGAACC CGCCTGTGCA 
GCAGCATGCA GTCAGAAAGT AGACAGCACT GTTTGCCACC TGTCTGCTAA AACTGAGAGT 
GAAGACCCCT CAGCAGTTCC TTCACCACAG CCACCAGTCG ATCATAGAGC ACAACCAGTC 
AAGAGCAAGG CCCATGACAT AAATCCTGAT GTCCTAAGCT CAGGTGTGCT CTGCCTGACT 
GGAACAAGAG ATAAATCAGG CCATGCCTTG GTCACTGTGA CAATGAGAAA CACTGTCTGG 
CTGAATCCAA ACTGCAACAG TGGAGAGCTA GTGCGAATCA TGGTGTATTT CTGCAGTACA 
CTCAGGAAGG AAGTAAGTGC TTTGGGATTG ACTGTCCTGG TGGATGCCCG CAGGTGTTCA 
CCTGTTCCCG CCCTTTTCAA AGCCTTCAAC ATCCTCCAGG AAGCCATGCC GGGATGCATT 
CATACTGTAC TGCTGCTGGC CGACAGAGAT CTGGCTCTAC GCATGGAGAA AACCTCTTCT 
ATACAGGTGG AGCTGCTGAC ATCACTCAAG TCTCTCTATA AACATGTGGA CGTTTCTCAG 
CTTCCCACTG AGTTTGAAGG CACTTTCCCT TATGCACACA GCAGCTGGGT CAGCTTTAGA 
ATGAGGTTGG AGCAGTTGAC CAGTCATTGT GAAGATGCTG TGAGCCTGCT TCAGAACACC 
ATAAGCAAAC TGGAGTCCAC AGTGCTGCCC CCTACAGCAG AGGAGGCTCA ACTCTTACTG 
TGTAAGTACA GAGAGCTGAT GAGGACGGTT CTGGAGGACA GCAGGCTGGT GCGACTACAG 
TTGGAGGGAG GAGCAGCTCT GTCCCGTTTA CGAAAAGAAG AGACCAGCGT TAGTCTCACA 
GAGGACTACA GAGATGCCAT TGAAAGTGCT GGCCGTTTGT ATAACCAAGT GGACGAGCTG 
GTTCACAGAC TGGTGATGCT GTCCAATAAA TGCACACAGG AACTGGAATT CATCATGGAG 
TTTAAGAGCC TGGAGGAGGG ATTCAAAGAG GTTAGTCAGT GGATAGAAGA GGTGGGAGAG 
TCTCGTCTGC AGACGCTGAG CGAGCTGGAG GATTCCTTGG AGCAGTTACA TCATAAGCAG 
AGCATCTTCA GAGAATTTTA CACAGCTGCA TATGAACACT GTAAGAGTGG TGAAGCTCTC 
CTCAAGCGTT TAGAGAAGTG GGAAGACGTC TCCTCTGCAG AGCTGCAAGT GTATGAGGTG 
AAAGTACGTT CATTCTGGGT GCATCTGCGG GATTTCTCTC AGCGAGTGGA GGACACCAAG 
GATAAAATAG ACAAGACTGT TCAACTCTAT GAGTTCTTTG ATAAGGCATA TGAATGGGCT 
CTAGAGGGCA TGCGTCATTT GGCGTGTGTC AGTATGGAGG ATTGTGGCAT GTCTGAAAAG 
TGTCAGAGCG TGATCACGTG TTTGGAGACT TACCGCAGTC AGCACCCCCC GATCCCTGAT 
GCACACTTCC AGGAGATGAA GGACCTCGCT GGGGAACTGA AAAGCGAACA GGGCCTCAAG 
CAGTGGAAGT TTGCCTGGTC CAAGTGTCAG GAGACCAAGC AGATGTTTGA AAAGAAGCTG 
GAGATGGCCC TGCGTTCCCG CCGTGACAGC GACTCCAAGT CAGTAAGGAG TGACTCTTCT 
CGTCGTAACT CAGATGTTAG CACTAAACAC CAAGAGCGTA GCAGTAGCAT CTCCTTCTCA 
TGTCGGAAAG CGTTCGGCGG AGCCTGGGGT CGAGATAGAC TTTCCTCACA TTCTAGCACA 
TCCTCAACTC CCAGTAGCCC TTCAGGCATT CGTCTGGATG CCCGTGACAC TGTCCGAACT 
CCAACAGGAA GTCCATACAG CATTGAGCAC AGAATCCCCC ACAGCACCCC TGTCAGATCC 
CACAGGCTAA CACGTAGCAT CTCCACAGAT GAGTCTCCTC AGAGCCCACA TGCAGAGGGG 
CTAGCGTCCA CAAGCCCAAG CGGCACTTCT GTTCAAACAA ACCGCAGGGT TTTGCGGAAG 
ACCCAGAGCT TCGATACAGC TGGCAGTGAG CCTGTGTCAC AGTACGGGAC CTGTCAGAGG 
ACTTTGAGTG AGCCTGCACG CAGAGGAAAC ACAGGGGTGT TCATTAAGGG TCTGGAGGTG 
AGCAGCACTG AAGTGATTGA TAGGCCGTAC AGTCCACGAC TACCACCCAT CCATGGATGG 
TCTTCAATTG ATGCACACCG CAGCGGGAGT CCTGCTCCAG AAACGCGCAG CAAGGGCAGC 
AAGTTACGAC ACATTGTAGA TGAGATGGTG ACAACAGAGC GGGAATATGT GCGTTCCCTG 
CGGTATATCA TTGACAACTA TTTCCCTGAG ATGGAGCGCG CTGTCCTGCC GCAGGATCTG 
CGGGGAAAGC GGAGCGTCAT ATTTGGAAAT CTGGAGAAAC TGGTGGACTT TCATAGTCAG 
TATTTCCTAA AAGAGCTGGA GAGCTGCTGT AATCATCCTC TACGCGTCAG CCACTGTTTC 
CTACGACATC AAGACCAGTT TAGCCTCTAT GCATTATACA GCAAGAACAA GCCAAAATCA 
GACACACTCC TGGCCAGCCA TGGAAACAAC TTCTTCAAGC ATAAGCAGAT GGAGCTGGGA 
GATAAGATGG ACCTGGCATC GTACCTGCTG AAGCCCATCC AGCGCATGAG TAAATACGCT 
CTTCTGCTGA AAGACCTGAT CAAAGAAGTG AGCGAGGTCC AGGAGCAGGA ACTTACATAC 
CTGCGAGCCG CAGCAGAGAT GGTCAAGTTT CAGCTCCGCC ATGGCAATGA TCTACTCGCC 
ATGGACGCTA TCAGAGATTG TGATGTGAAC CTAAAAGAGC AAGGACAGTT GGTTCGTCAA 
GATGAGTTCA CGATTTCATA TGGCAGAAAG AAGTGCCAGA GACACGTGTT CCTATTTGAG 
GACCTAGTGC TCTTCAGCAA ACCCAAGCGC ATCGAGGGTG GACTAGATGT TTACATATAC 
AAGCATTCTT TTAAGACGGC TGATGTGGGT ATGACAGAGA CGTCAGGTGA GAACAGTCTG 
AGGTTTGAAA TTTGGTTTCG GAGGAGAACT TCAAAGAACC AGACCTACAT TCTCCAGTCC 
AGCACTGCTG ACATCAAACA TGCCTGGACT TGCGACATCG CACGAATACT GTGGCAGCAG 
GCCACGCGAA ACAAAGAAAT TCGAATGCAG GAGATGGTCT CAATGGGTGT GGGCAATAAA 
CCCTTCCTGG ACATTAAACC CAGTGATGCT GCAATCAACG ACAGAGCCAT CGACTACATT 
ATGAAGGGCA GAGGAGCTAG AACGAGAGCC TCCATTGCTG TTTCTCTGTT TGACCACTCA 
AACCCATTCA AAAGAGCTGC AGTAAACACC CCCGTCAGCG GGACCCCGGT GTCTGGCGGT 
CCTTCCTCAT CCACCCTGCT GGGGCCCTTG AATCTGCACA TGTACAGCAG CCAAAGTCTG 
TTGGCGGGGG AGAGACCTCT GATTGGCCCC TGCATCGAGG AGGACGAGCT GGAGCATGAG 
ACCAGTAGTC AGCCCTCTAT GACTACAGAG AGTTCGGGTT CGTCGTCTCA CTGTCTGTCT 
GGCAGTGGCT CTAGCGGATC AGACAGCGGC TGTGTGTCCA GTCACCTTCC TGAGGCTTTG 
TCTGAGGAGC CCAGCTCACC TTGTGACTCC TCGTGTTATT CCTCCATCAC CTCTCCCACG 
CAGGAGAAGC CCTGCTTTAA CAGCCAGTAT ATCTCAGCGG GGGATGCTCA GGTGATCGGA 
CCCTCTACTA TAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333590.1
CDS	173..5050
Translation	MDSESLDSCIQSTLSALYPPFEATAATVLCQVLDVVETTYRGDGLRYIIDFLVPAKHILQSIQQDACFPYCGFLFRHEGWPLCVHEKVIIQLSSLDWRVLRPSDFYLQVTPSAKSPPRIKVKCLAVTGQHAEELDVPELAYTSLFTMDWLDSINQERSVVRRAALEHCLLSADNDIFRVPWEDIVHPEFISRPSKVAEQSESRRVAKEVANGDGRDSDVEQDNNPEDIQCRIFYDTSTNHSGDMDCQSKEVKLLPVLSEINKDISGRSSSSTADDSEGEYVELADISLPRFSPQKGSLTQAISMNYRNLHKPQTPVSTQLKTKPDESLLKNGACSQSMVCAMMIEESLSDTYQTVILTPTAPAEAERPLQQVDMSEPACAAACSQKVDSTVCHLSAKTESEDPSAVPSPQPPVDHRAQPVKSKAHDINPDVLSSGVLCLTGTRDKSGHALVTVTMRNTVWLNPNCNSGELVRIMVYFCSTLRKEVSALGLTVLVDARRCSPVPALFKAFNILQEAMPGCIHTVLLLADRDLALRMEKTSSIQVELLTSLKSLYKHVDVSQLPTEFEGTFPYAHSSWVSFRMRLEQLTSHCEDAVSLLQNTISKLESTVLPPTAEEAQLLLCKYRELMRTVLEDSRLVRLQLEGGAALSRLRKEETSVSLTEDYRDAIESAGRLYNQVDELVHRLVMLSNKCTQELEFIMEFKSLEEGFKEVSQWIEEVGESRLQTLSELEDSLEQLHHKQSIFREFYTAAYEHCKSGEALLKRLEKWEDVSSAELQVYEVKVRSFWVHLRDFSQRVEDTKDKIDKTVQLYEFFDKAYEWALEGMRHLACVSMEDCGMSEKCQSVITCLETYRSQHPPIPDAHFQEMKDLAGELKSEQGLKQWKFAWSKCQETKQMFEKKLEMALRSRRDSDSKSVRSDSSRRNSDVSTKHQERSSSISFSCRKAFGGAWGRDRLSSHSSTSSTPSSPSGIRLDARDTVRTPTGSPYSIEHRIPHSTPVRSHRLTRSISTDESPQSPHAEGLASTSPSGTSVQTNRRVLRKTQSFDTAGSEPVSQYGTCQRTLSEPARRGNTGVFIKGLEVSSTEVIDRPYSPRLPPIHGWSSIDAHRSGSPAPETRSKGSKLRHIVDEMVTTEREYVRSLRYIIDNYFPEMERAVLPQDLRGKRSVIFGNLEKLVDFHSQYFLKELESCCNHPLRVSHCFLRHQDQFSLYALYSKNKPKSDTLLASHGNNFFKHKQMELGDKMDLASYLLKPIQRMSKYALLLKDLIKEVSEVQEQELTYLRAAAEMVKFQLRHGNDLLAMDAIRDCDVNLKEQGQLVRQDEFTISYGRKKCQRHVFLFEDLVLFSKPKRIEGGLDVYIYKHSFKTADVGMTETSGENSLRFEIWFRRRTSKNQTYILQSSTADIKHAWTCDIARILWQQATRNKEIRMQEMVSMGVGNKPFLDIKPSDAAINDRAIDYIMKGRGARTRASIAVSLFDHSNPFKRAAVNTPVSGTPVSGGPSSSTLLGPLNLHMYSSQSLLAGERPLIGPCIEEDELEHETSSQPSMTTESSGSSSHCLSGSGSSGSDSGCVSSHLPEALSEEPSSPCDSSCYSSITSPTQEKPCFNSQYISAGDAQVIGPSTIV

Target ORF information:

RefSeq Version	XM_021477915.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477915.1

ORF Insert Sequence:

ATGGACTCTG AATCTCTGGA CAGCTGTATC CAGAGCACAC TGTCCGCTCT CTACCCTCCA 
TTTGAAGCCA CTGCGGCCAC TGTACTGTGC CAGGTGTTAG ATGTGGTGGA GACAACATAC 
CGTGGAGACG GACTGCGATA CATCATCGAC TTTCTGGTGC CCGCTAAACA CATCCTACAG 
AGCATTCAGC AGGATGCATG CTTCCCATAC TGTGGTTTTC TGTTCCGTCA TGAAGGATGG 
CCACTGTGTG TCCATGAGAA GGTCATAATC CAGCTCTCCT CTTTGGACTG GCGTGTATTA 
CGGCCCAGCG ACTTCTACTT GCAGGTGACA CCATCTGCCA AAAGCCCCCC GCGCATCAAA 
GTGAAGTGTC TGGCTGTTAC CGGGCAGCAT GCGGAGGAGC TGGACGTGCC GGAGCTAGCA 
TATACCTCTC TATTCACCAT GGACTGGCTG GACTCCATCA ACCAGGAGAG GAGTGTGGTC 
AGAAGAGCTG CCCTGGAACA CTGTCTTTTA TCAGCGGATA ATGATATTTT TAGGGTACCG 
TGGGAGGATA TTGTTCACCC GGAATTTATC AGCAGACCCT CTAAAGTTGC AGAACAAAGT 
GAAAGTAGAA GGGTTGCCAA AGAAGTTGCA AATGGAGACG GGCGAGACTC TGATGTAGAA 
CAAGACAACA ATCCTGAGGA CATACAATGT AGAATTTTTT ATGACACTTC CACGAACCAT 
TCAGGGGATA TGGATTGTCA GAGCAAGGAA GTTAAGCTCC TCCCTGTTCT TAGTGAGATT 
AATAAAGATA TCAGTGGGAG GAGCTCTAGC AGCACTGCGG ACGATTCAGA GGGCGAATAT 
GTGGAGCTAG CTGATATCTC CTTACCACGC TTTTCCCCAC AAAAGGGCTC TCTTACTCAA 
GCCATTAGCA TGAACTATAG AAATCTACAT AAACCTCAGA CTCCTGTTTC CACTCAGCTT 
AAAACCAAAC CAGATGAGAG TCTTTTGAAG AATGGTGCAT GCTCTCAAAG CATGGTGTGT 
GCCATGATGA TCGAGGAAAG CCTGAGTGAT ACCTACCAAA CAGTGATCCT TACTCCCACT 
GCTCCTGCAG AGGCAGAGCG TCCGCTCCAG CAGGTGGACA TGTCTGAACC CGCCTGTGCA 
GCAGCATGCA GTCAGAAAGT AGACAGCACT GTTTGCCACC TGTCTGCTAA AACTGAGAGT 
GAAGACCCCT CAGCAGTTCC TTCACCACAG CCACCAGTCG ATCATAGAGC ACAACCAGTC 
AAGAGCAAGG CCCATGACAT AAATCCTGAT GTCCTAAGCT CAGGTGTGCT CTGCCTGACT 
GGAACAAGAG ATAAATCAGG CCATGCCTTG GTCACTGTGA CAATGAGAAA CACTGTCTGG 
CTGAATCCAA ACTGCAACAG TGGAGAGCTA GTGCGAATCA TGGTGTATTT CTGCAGTACA 
CTCAGGAAGG AAGTAAGTGC TTTGGGATTG ACTGTCCTGG TGGATGCCCG CAGGTGTTCA 
CCTGTTCCCG CCCTTTTCAA AGCCTTCAAC ATCCTCCAGG AAGCCATGCC GGGATGCATT 
CATACTGTAC TGCTGCTGGC CGACAGAGAT CTGGCTCTAC GCATGGAGAA AACCTCTTCT 
ATACAGGTGG AGCTGCTGAC ATCACTCAAG TCTCTCTATA AACATGTGGA CGTTTCTCAG 
CTTCCCACTG AGTTTGAAGG CACTTTCCCT TATGCACACA GCAGCTGGGT CAGCTTTAGA 
ATGAGGTTGG AGCAGTTGAC CAGTCATTGT GAAGATGCTG TGAGCCTGCT TCAGAACACC 
ATAAGCAAAC TGGAGTCCAC AGTGCTGCCC CCTACAGCAG AGGAGGCTCA ACTCTTACTG 
TGTAAGTACA GAGAGCTGAT GAGGACGGTT CTGGAGGACA GCAGGCTGGT GCGACTACAG 
TTGGAGGGAG GAGCAGCTCT GTCCCGTTTA CGAAAAGAAG AGACCAGCGT TAGTCTCACA 
GAGGACTACA GAGATGCCAT TGAAAGTGCT GGCCGTTTGT ATAACCAAGT GGACGAGCTG 
GTTCACAGAC TGGTGATGCT GTCCAATAAA TGCACACAGG AACTGGAATT CATCATGGAG 
TTTAAGAGCC TGGAGGAGGG ATTCAAAGAG GTTAGTCAGT GGATAGAAGA GGTGGGAGAG 
TCTCGTCTGC AGACGCTGAG CGAGCTGGAG GATTCCTTGG AGCAGTTACA TCATAAGCAG 
AGCATCTTCA GAGAATTTTA CACAGCTGCA TATGAACACT GTAAGAGTGG TGAAGCTCTC 
CTCAAGCGTT TAGAGAAGTG GGAAGACGTC TCCTCTGCAG AGCTGCAAGT GTATGAGGTG 
AAAGTACGTT CATTCTGGGT GCATCTGCGG GATTTCTCTC AGCGAGTGGA GGACACCAAG 
GATAAAATAG ACAAGACTGT TCAACTCTAT GAGTTCTTTG ATAAGGCATA TGAATGGGCT 
CTAGAGGGCA TGCGTCATTT GGCGTGTGTC AGTATGGAGG ATTGTGGCAT GTCTGAAAAG 
TGTCAGAGCG TGATCACGTG TTTGGAGACT TACCGCAGTC AGCACCCCCC GATCCCTGAT 
GCACACTTCC AGGAGATGAA GGACCTCGCT GGGGAACTGA AAAGCGAACA GGGCCTCAAG 
CAGTGGAAGT TTGCCTGGTC CAAGTGTCAG GAGACCAAGC AGATGTTTGA AAAGAAGCTG 
GAGATGGCCC TGCGTTCCCG CCGTGACAGC GACTCCAAGT CAGTAAGGAG TGACTCTTCT 
CGTCGTAACT CAGATGTTAG CACTAAACAC CAAGAGCGTA GCAGTAGCAT CTCCTTCTCA 
TGTCGGAAAG CGTTCGGCGG AGCCTGGGGT CGAGATAGAC TTTCCTCACA TTCTAGCACA 
TCCTCAACTC CCAGTAGCCC TTCAGGCATT CGTCTGGATG CCCGTGACAC TGTCCGAACT 
CCAACAGGAA GTCCATACAG CATTGAGCAC AGAATCCCCC ACAGCACCCC TGTCAGATCC 
CACAGGCTAA CACGTAGCAT CTCCACAGAT GAGTCTCCTC AGAGCCCACA TGCAGAGGGG 
CTAGCGTCCA CAAGCCCAAG CGGCACTTCT GTTCAAACAA ACCGCAGGGT TTTGCGGAAG 
ACCCAGAGCT TCGATACAGC TGGCAGTGAG CCTGTGTCAC AGTACGGGAC CTGTCAGAGG 
ACTTTGAGTG AGCCTGCACG CAGAGGAAAC ACAGGGGTGT TCATTAAGGG TCTGGAGGTG 
AGCAGCACTG AAGTGATTGA TAGGCCGTAC AGTCCACGAC TACCACCCAT CCATGGATGG 
TCTTCAATTG ATGCACACCG CAGCGGGAGT CCTGCTCCAG AAACGCGCAG CAAGGGCAGC 
AAGTTACGAC ACATTGTAGA TGAGATGGTG ACAACAGAGC GGGAATATGT GCGTTCCCTG 
CGGTATATCA TTGACAACTA TTTCCCTGAG ATGGAGCGCG CTGTCCTGCC GCAGGATCTG 
CGGGGAAAGC GGAGCGTCAT ATTTGGAAAT CTGGAGAAAC TGGTGGACTT TCATAGTCAG 
TATTTCCTAA AAGAGCTGGA GAGCTGCTGT AATCATCCTC TACGCGTCAG CCACTGTTTC 
CTACGACATC AAGACCAGTT TAGCCTCTAT GCATTATACA GCAAGAACAA GCCAAAATCA 
GACACACTCC TGGCCAGCCA TGGAAACAAC TTCTTCAAGC ATAAGCAGAT GGAGCTGGGA 
GATAAGATGG ACCTGGCATC GTACCTGCTG AAGCCCATCC AGCGCATGAG TAAATACGCT 
CTTCTGCTGA AAGACCTGAT CAAAGAAGTG AGCGAGGTCC AGGAGCAGGA ACTTACATAC 
CTGCGAGCCG CAGCAGAGAT GGTCAAGTTT CAGCTCCGCC ATGGCAATGA TCTACTCGCC 
ATGGACGCTA TCAGAGATTG TGATGTGAAC CTAAAAGAGC AAGGACAGTT GGTTCGTCAA 
GATGAGTTCA CGATTTCATA TGGCAGAAAG AAGTGCCAGA GACACGTGTT CCTATTTGAG 
GACCTAGTGC TCTTCAGCAA ACCCAAGCGC ATCGAGGGTG GACTAGATGT TTACATATAC 
AAGCATTCTT TTAAGACGGC TGATGTGGGT ATGACAGAGA CGTCAGGTGA GAACAGTCTG 
AGGTTTGAAA TTTGGTTTCG GAGGAGAACT TCAAAGAACC AGACCTACAT TCTCCAGTCC 
AGCACTGCTG ACATCAAACA TGCCTGGACT TGCGACATCG CACGAATACT GTGGCAGCAG 
GCCACGCGAA ACAAAGAAAT TCGAATGCAG GAGATGGTCT CAATGGGTGT GGGCAATAAA 
CCCTTCCTGG ACATTAAACC CAGTGATGCT GCAATCAACG ACAGAGCCAT CGACTACATT 
ATGAAGGGCA GAGGAGCTAG AACGAGAGCC TCCATTGCTG TTTCTCTGTT TGACCACTCA 
AACCCATTCA AAAGAGCTGC AGTAAACACC CCCGTCAGCG GGACCCCGGT GTCTGGCGGT 
CCTTCCTCAT CCACCCTGCT GGGGCCCTTG AATCTGCACA TGTACAGCAG CCAAAGTCTG 
TTGGCGGGGG AGAGACCTCT GATTGGCCCC TGCATCGAGG AGGACGAGCT GGAGCATGAG 
ACCAGTAGTC AGCCCTCTAT GACTACAGAG AGTTCGGGTT CGTCGTCTCA CTGTCTGTCT 
GGCAGTGGCT CTAGCGGATC AGACAGCGGC TGTGTGTCCA GTCACCTTCC TGAGGCTTTG 
TCTGAGGAGC CCAGCTCACC TTGTGACTCC TCGTGTTATT CCTCCATCAC CTCTCCCACG 
CAGGAGAAGC CCTGCTTTAA CAGCCAGTAT ATCTCAGCGG GGGATGCTCA GGTGATCGGA 
CCCTCTACTA TAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg4 ( XM_009303344.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301619.1 Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X1, mRNA.

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CloneID	ODa39866
Clone ID Related Accession (Same CDS sequence)	XM_009303344.3 , XM_009303344.2
Accession Version	XM_009303344.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein plekhg4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303344.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTG AATCTCTGGA CAGCTGTATC CAGAGCACAC TGTCCGCTCT CTACCCTCCA 
TTTGAAGCCA CTGCGGCCAC TGTACTGTGC CAGGTGTTAG ATGTGGTGGA GACAACATAC 
CGTGGAGACG GACTGCGATA CATCATCGAC TTTCTGGTGC CCGCTAAACA CATCCTACAG 
AGCATTCAGC AGGATGCATG CTTCCCATAC TGTGGTTTTC TGTTCCGTCA TGAAGGATGG 
CCACTGTGTG TCCATGAGAA GGTCATAATC CAGCTCTCCT CTTTGGACTG GCGTGTATTA 
CGGCCCAGCG ACTTCTACTT GCAGGTGACA CCATCTGCCA AAAGCCCCCC GCGCATCAAA 
GTGAAGTGTC TGGCTGTTAC CGGGCAGCAT GCGGAGGAGC TGGACGTGCC GGAGCTAGCA 
TATACCTCTC TATTCACCAT GGACTGGCTG GACTCCATCA ACCAGGAGAG GAGTGTGGTC 
AGAAGAGCTG CCCTGGAACA CTGTCTTTTA TCAGCGGATA ATGATATTTT TAGGGTACCG 
TGGGAGGATA TTGTTCACCC GGAATTTATC AGCAGACCCT CTAAAGTTGC AGAACAAAGT 
GAAAGTAGAA GGGTTGCCAA AGAAGTTGCA AATGGAGACG GGCGAGACTC TGATGTAGAA 
CAAGACAACA ATCCTGAGGA CATACAATGT AGAATTTTTT ATGACACTTC CACGAACCAT 
TCAGGGGATA TGGATTGTCA GAGCAAGGAA GTTAAGCTCC TCCCTGTTCT TAGTGAGATT 
AATAAAGATA TCAGTGGGAG GAGCTCTAGC AGCACTGCGG ACGATTCAGA GGGCGAATAT 
GTGGAGCTAG CTGATATCTC CTTACCACGC TTTTCCCCAC AAAAGGGCTC TCTTACTCAA 
GCCATTAGCA TGAACTATAG AAATCTACAT AAACCTCAGA CTCCTGTTTC CACTCAGCTT 
AAAACCAAAC CAGATGAGAG TCTTTTGAAG AATGGTGCAT GCTCTCAAAG CATGGTGTGT 
GCCATGATGA TCGAGGAAAG CCTGAGTGAT ACCTACCAAA CAGTGATCCT TACTCCCACT 
GCTCCTGCAG AGGCAGAGCG TCCGCTCCAG CAGGTGGACA TGTCTGAACC CGCCTGTGCA 
GCAGGTACAG CTTCCTTTGA CAGTGTGTCT GAGCATGAGT CTGAGAGAGA GCTATTATCT 
CAACACTCTG ACTGTGAGGA TGCTTCACTA TCTGATATAT CAACAATTAA CATATCGCAA 
ACAGACATTA AACCAATCAA CACATCACAA CTAGACAACA ATGTAGTCTG CAAATCACAA 
GACAGCGAAC CATACAGTAC ATCACAATTA GACAAGAATC TAGTGAACAC ATCACAAACA 
GATACTGAAT CATTGAGCAT GTCTCAATTA GACGATAATT TGGACTGCAC ATCACATCCA 
GATGTTAACC AGAAACCATC AAGTATGTCT CAACTAGAAC TTGAACCACT AACCGTATCT 
CAGCTTGACA GCAAGCTAAT CAGCACATCA CAACCTGATA GCACATCACT AGATGTAGAA 
CCAGTTAGCA CATCTAATGT AGACAACAAA CCAGTCAGCA CATCACAACC GGACAGCACA 
TTACAGTTGG TCAATGAACC AGTCAAGACA TCACAAGATG GTGAACCAGT AAGCAGATTT 
CATGTAGACC ACAAACCAGT AAGCACATTA CAACCAGACA GCCAACCAGT CATCACATCA 
GTCAATGAGC CATTTAGGAC ATCACAACCA GACAATAAAT CAGTAAGCAC AACTCAACTA 
GACAGCAAAC CAGTTAGTGT ATCACAACAC AACAAACAAG AAAGCAAATC TGTCAACACA 
TCTGTTGACA ATGAGCCAGT CAAGACATCA CAGCCAGACA CTGAATCACT AAGAATATCT 
CAATTTGACA GCAAACCAGT CAGCAACTCA CAACTAGGCA GCAAACAAGT CAGCATTAAA 
CAACTAGTCA ATGAGATATT TAGTACACCA CAACCAGACA ATAAATCAGT GAGCACAACT 
CAACTAGAAA GAAAACTAGT TTGCGGATCA CAAGACAACA AACAAGAAAG CAAATCTGTC 
AACACATTAG TAGACAGTGA GCCAGTCAAG ACATCACAGA CAGACACAGA ACTGCTAAGA 
ATATCACAGT TTGATAGCAA ACCAGTCAGC AACTCACAAC TAGATGGCAA ACAAGTCAGC 
ATTTCACAGC TAGTCAATGA GATATTTAGT ACACCACAAC CAGACAATAA ATCAGTAAGC 
CCAACTCAAC TAGACAGTAA GCTAGTTAGC ACATTACAAG ACAAACAAGA AAGCAAATCT 
GTCAATAATT CAATAGACAA TGAGCCAGTC AAGACATCAC AGGCAGACAC AGAACCGCTA 
AAAAGGTCTC AGTTTGACAG CAACCCAGTC AGCAACTCAC AGCTAGATGG CAAACAAGTC 
AGCATTTCAC AACTAGTCAA TGAGATATTT AGTACACCAC AACCAGACAA TAAATCAGTG 
AGCACAACTC AACTAGATGG CAACATAGAT AGAACATCAC AATTAGACAG CAAACAAGAA 
AGTGTCTCTC AAACAGACAG CAAACCAGTC AGCATATCAC CAGACAATGA GCCAGTGAAG 
ACATCACAAC CACAACTAGA AAGCACATTA CACCCAAACA GCAAACAAGC AAGCATGTCA 
CAACTAGTCA ATGAACACAT CCAGACCTCA CAGCCAGACC TAAAACCAGT AAGCATTGCT 
CAACTCTACA GTGAAGATGC TAAACCAGAT GGATACGCTG ATGTCAAACC AATAACCACT 
GAGCTAGACA ATGGACTAGT GAGCACATCA CAACCAGATA ACCAACCAGT TGACACACCA 
CAAGCAGAAT GTACAACAGT TAACATAACC TTACCAGAGG GCAACCCCAA TGACACATCA 
TATATTGTCA ACCTGCCGCT AAGTACATCA CAACCAAACA GCAAAACAGT TCATGTGGAG 
CAAGCAGACA TAAATGTAAT TAATACATCA CAGCCAGAGA CTGAAATGTT ATCTTGTGTT 
TTGGAAAGGG AGAACTCAGA ACAGGTTTCT CCAGCTTATA TTGAACTCAT TGTGTCAGAT 
CCTTATGCAG AAGTCAGATG TGAATCTAAA GAGCTGAGAG TTAATTGTGA TCTTGAAGGC 
AGTTGTGGTT CACAGAACAA CAGAGCGTCA CTTGCCGAGA CAGTAGCTGA GAGTGTGTCT 
TCGCAAAATC AGATTGATCA AACAGGCAAT AGCCTGTATC AGATACACAA ACCCATCCAT 
ACACCATCTG AGCAAATGCA TGATCTTCCT ACCATAAAAG AAGACCATCA GACATTCAGT 
AGCAGTTCTC ATAACACGAA TGAATCTGTT GGTGATTTAA GTCCCATTGC AGAAGAAGAT 
GGTGGGTCTG TTACAGCTGC TCTGTATAGT GAGCATGTAG CCTTAGTTTC TGTCACCACA 
GTGCCTGGGG AGAGTCCAGT TACACTCATT TCACAGGGTC AGGTGGGGAC AGAGTCTCCT 
GAGGACAGAA TCAACCAGAC AGAGGTGGAA CTTCAGACAA AGGAAGAACG TGCAGTACAA 
AACGTAGCTG GAATGCCACC TTGTAAGGGG GCTGAAATGG TAGAAAAGAA AAGCAGTAGC 
GAGGCAGAAA TCAAAACTGA TGAGAATGAT GTAGTGGCAG TGTGTAGGCA AGAGGAAGTT 
GAGACTTCAC AAACAGGTCT AGTAGATGCT ACGCCCAAGT CTGACGTGCA AGTTCAGGAG 
GAGGTCAGCT CCCCTGTAGA AACAGATGAG AAGAAACAAA CCAGTGTTCA AGAAAAAGGG 
GTGGAGGAAG AGGAGGTCAA GGCCAAAACT GTGAGGGAGC AAGAGGCCAT AGTGACCAGC 
AATTCTGAAA ATAGAGTCCA GTCAGCATGC TCTGAAGTGC TTGATCCAGC CTCTGATGTC 
CCGAACACAA ATGGACACAT GCATGCTCAG GACACTCCTA CCACTGAACT AAAAGGGCAA 
ATAACAAAGG AGGAGGATGG AAAAGGGGCA GAGGAGAAAA GGCAAGAGGA TGAGATTGCT 
TCTTCTGTCA ATGGCTCTCA AAAAGAGCAT CAGCAGCAAG CAGACATGGA CTCCAGCTTC 
CATCAGCAGG AAGAGCAAGC ATGCAGTCAG AAAGTAGACA GCACTGTTTG CCACCTGTCT 
GCTAAAACTG AGAGTGAAGA CCCCTCAGCA GTTCCTTCAC CACAGCCACC AGTCGATCAT 
AGAGCACAAC CAGTCAAGAG CAAGGCCCAT GACATAAATC CTGATGTCCT AAGCTCAGGT 
GTGCTCTGCC TGACTGGAAC AAGAGATAAA TCAGGCCATG CCTTGGTCAC TGTGACAATG 
AGAAACACTG TCTGGCTGAA TCCAAACTGC AACAGTGGAG AGCTAGTGCG AATCATGGTG 
TATTTCTGCA GTACACTCAG GAAGGAAGTA AGTGCTTTGG GATTGACTGT CCTGGTGGAT 
GCCCGCAGGT GTTCACCTGT TCCCGCCCTT TTCAAAGCCT TCAACATCCT CCAGGAAGCC 
ATGCCGGGAT GCATTCATAC TGTACTGCTG CTGGCCGACA GAGATCTGGC TCTACGCATG 
GAGAAAACCT CTTCTATACA GGTGGAGCTG CTGACATCAC TCAAGTCTCT CTATAAACAT 
GTGGACGTTT CTCAGCTTCC CACTGAGTTT GAAGGCACTT TCCCTTATGC ACACAGCAGC 
TGGGTCAGCT TTAGAATGAG GTTGGAGCAG TTGACCAGTC ATTGTGAAGA TGCTGTGAGC 
CTGCTTCAGA ACACCATAAG CAAACTGGAG TCCACAGTGC TGCCCCCTAC AGCAGAGGAG 
GCTCAACTCT TACTGTGTAA GTACAGAGAG CTGATGAGGA CGGTTCTGGA GGACAGCAGG 
CTGGTGCGAC TACAGTTGGA GGGAGGAGCA GCTCTGTCCC GTTTACGAAA AGAAGAGACC 
AGCGTTAGTC TCACAGAGGA CTACAGAGAT GCCATTGAAA GTGCTGGCCG TTTGTATAAC 
CAAGTGGACG AGCTGGTTCA CAGACTGGTG ATGCTGTCCA ATAAATGCAC ACAGGAACTG 
GAATTCATCA TGGAGTTTAA GAGCCTGGAG GAGGGATTCA AAGAGGTTAG TCAGTGGATA 
GAAGAGGTGG GAGAGTCTCG TCTGCAGACG CTGAGCGAGC TGGAGGATTC CTTGGAGCAG 
TTACATCATA AGCAGAGCAT CTTCAGAGAA TTTTACACAG CTGCATATGA ACACTGTAAG 
AGTGGTGAAG CTCTCCTCAA GCGTTTAGAG AAGTGGGAAG ACGTCTCCTC TGCAGAGCTG 
CAAGTGTATG AGGTGAAAGT ACGTTCATTC TGGGTGCATC TGCGGGATTT CTCTCAGCGA 
GTGGAGGACA CCAAGGATAA AATAGACAAG ACTGTTCAAC TCTATGAGTT CTTTGATAAG 
GCATATGAAT GGGCTCTAGA GGGCATGCGT CATTTGGCGT GTGTCAGTAT GGAGGATTGT 
GGCATGTCTG AAAAGTGTCA GAGCGTGATC ACGTGTTTGG AGACTTACCG CAGTCAGCAC 
CCCCCGATCC CTGATGCACA CTTCCAGGAG ATGAAGGACC TCGCTGGGGA ACTGAAAAGC 
GAACAGGGCC TCAAGCAGTG GAAGTTTGCC TGGTCCAAGT GTCAGGAGAC CAAGCAGATG 
TTTGAAAAGA AGCTGGAGAT GGCCCTGCGT TCCCGCCGTG ACAGCGACTC CAAGTCAGTA 
AGGAGTGACT CTTCTCGTCG TAACTCAGAT GTTAGCACTA AACACCAAGA GCGTAGCAGT 
AGCATCTCCT TCTCATGTCG GAAAGCGTTC GGCGGAGCCT GGGGTCGAGA TAGACTTTCC 
TCACATTCTA GCACATCCTC AACTCCCAGT AGCCCTTCAG GCATTCGTCT GGATGCCCGT 
GACACTGTCC GAACTCCAAC AGGAAGTCCA TACAGCATTG AGCACAGAAT CCCCCACAGC 
ACCCCTGTCA GATCCCACAG GCTAACACGT AGCATCTCCA CAGATGAGTC TCCTCAGAGC 
CCACATGCAG AGGGGCTAGC GTCCACAAGC CCAAGCGGCA CTTCTGTTCA AACAAACCGC 
AGGGTTTTGC GGAAGACCCA GAGCTTCGAT ACAGCTGGCA GTGAGCCTGT GTCACAGTAC 
GGGACCTGTC AGAGGACTTT GAGTGAGCCT GCACGCAGAG GAAACACAGG GGTGTTCATT 
AAGGGTCTGG AGGTGAGCAG CACTGAAGTG ATTGATAGGC CGTACAGTCC ACGACTACCA 
CCCATCCATG GATGGTCTTC AATTGATGCA CACCGCAGCG GGAGTCCTGC TCCAGAAACG 
CGCAGCAAGG GCAGCAAGTT ACGACACATT GTAGATGAGA TGGTGACAAC AGAGCGGGAA 
TATGTGCGTT CCCTGCGGTA TATCATTGAC AACTATTTCC CTGAGATGGA GCGCGCTGTC 
CTGCCGCAGG ATCTGCGGGG AAAGCGGAGC GTCATATTTG GAAATCTGGA GAAACTGGTG 
GACTTTCATA GTCAGTATTT CCTAAAAGAG CTGGAGAGCT GCTGTAATCA TCCTCTACGC 
GTCAGCCACT GTTTCCTACG ACATCAAGAC CAGTTTAGCC TCTATGCATT ATACAGCAAG 
AACAAGCCAA AATCAGACAC ACTCCTGGCC AGCCATGGAA ACAACTTCTT CAAGCATAAG 
CAGATGGAGC TGGGAGATAA GATGGACCTG GCATCGTACC TGCTGAAGCC CATCCAGCGC 
ATGAGTAAAT ACGCTCTTCT GCTGAAAGAC CTGATCAAAG AAGTGAGCGA GGTCCAGGAG 
CAGGAACTTA CATACCTGCG AGCCGCAGCA GAGATGGTCA AGTTTCAGCT CCGCCATGGC 
AATGATCTAC TCGCCATGGA CGCTATCAGA GATTGTGATG TGAACCTAAA AGAGCAAGGA 
CAGTTGGTTC GTCAAGATGA GTTCACGATT TCATATGGCA GAAAGAAGTG CCAGAGACAC 
GTGTTCCTAT TTGAGGACCT AGTGCTCTTC AGCAAACCCA AGCGCATCGA GGGTGGACTA 
GATGTTTACA TATACAAGCA TTCTTTTAAG ACGGCTGATG TGGGTATGAC AGAGACGTCA 
GGTGAGAACA GTCTGAGGTT TGAAATTTGG TTTCGGAGGA GAACTTCAAA GAACCAGACC 
TACATTCTCC AGTCCAGCAC TGCTGACATC AAACATGCCT GGACTTGCGA CATCGCACGA 
ATACTGTGGC AGCAGGCCAC GCGAAACAAA GAAATTCGAA TGCAGGAGAT GGTCTCAATG 
GGTGTGGGCA ATAAACCCTT CCTGGACATT AAACCCAGTG ATGCTGCAAT CAACGACAGA 
GCCATCGACT ACATTATGAA GGGCAGAGGA GCTAGAACGA GAGCCTCCAT TGCTGTTTCT 
CTGTTTGACC ACTCAAACCC ATTCAAAAGA GCTGCAGTAA ACACCCCCGT CAGCGGGACC 
CCGGTGTCTG GCGGTCCTTC CTCATCCACC CTGCTGGGGC CCTTGAATCT GCACATGTAC 
AGCAGCCAAA GTCTGTTGGC GGGGGAGAGA CCTCTGATTG GCCCCTGCAT CGAGGAGGAC 
GAGCTGGAGC ATGAGACCAG TAGTCAGCCC TCTATGACTA CAGAGAGTTC GGGTTCGTCG 
TCTCACTGTC TGTCTGGCAG TGGCTCTAGC GGATCAGACA GCGGCTGTGT GTCCAGTCAC 
CTTCCTGAGG CTTTGTCTGA GGAGCCCAGC TCACCTTGTG ACTCCTCGTG TTATTCCTCC 
ATCACCTCTC CCACGCAGGA GAAGCCCTGC TTTAACAGCC AGTATATCTC AGCGGGGGAT 
GCTCAGGTGA TCGGACCCTC TACTATAGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301619.1
CDS	175..8067
Translation	MDSESLDSCIQSTLSALYPPFEATAATVLCQVLDVVETTYRGDGLRYIIDFLVPAKHILQSIQQDACFPYCGFLFRHEGWPLCVHEKVIIQLSSLDWRVLRPSDFYLQVTPSAKSPPRIKVKCLAVTGQHAEELDVPELAYTSLFTMDWLDSINQERSVVRRAALEHCLLSADNDIFRVPWEDIVHPEFISRPSKVAEQSESRRVAKEVANGDGRDSDVEQDNNPEDIQCRIFYDTSTNHSGDMDCQSKEVKLLPVLSEINKDISGRSSSSTADDSEGEYVELADISLPRFSPQKGSLTQAISMNYRNLHKPQTPVSTQLKTKPDESLLKNGACSQSMVCAMMIEESLSDTYQTVILTPTAPAEAERPLQQVDMSEPACAAGTASFDSVSEHESERELLSQHSDCEDASLSDISTINISQTDIKPINTSQLDNNVVCKSQDSEPYSTSQLDKNLVNTSQTDTESLSMSQLDDNLDCTSHPDVNQKPSSMSQLELEPLTVSQLDSKLISTSQPDSTSLDVEPVSTSNVDNKPVSTSQPDSTLQLVNEPVKTSQDGEPVSRFHVDHKPVSTLQPDSQPVITSVNEPFRTSQPDNKSVSTTQLDSKPVSVSQHNKQESKSVNTSVDNEPVKTSQPDTESLRISQFDSKPVSNSQLGSKQVSIKQLVNEIFSTPQPDNKSVSTTQLERKLVCGSQDNKQESKSVNTLVDSEPVKTSQTDTELLRISQFDSKPVSNSQLDGKQVSISQLVNEIFSTPQPDNKSVSPTQLDSKLVSTLQDKQESKSVNNSIDNEPVKTSQADTEPLKRSQFDSNPVSNSQLDGKQVSISQLVNEIFSTPQPDNKSVSTTQLDGNIDRTSQLDSKQESVSQTDSKPVSISPDNEPVKTSQPQLESTLHPNSKQASMSQLVNEHIQTSQPDLKPVSIAQLYSEDAKPDGYADVKPITTELDNGLVSTSQPDNQPVDTPQAECTTVNITLPEGNPNDTSYIVNLPLSTSQPNSKTVHVEQADINVINTSQPETEMLSCVLERENSEQVSPAYIELIVSDPYAEVRCESKELRVNCDLEGSCGSQNNRASLAETVAESVSSQNQIDQTGNSLYQIHKPIHTPSEQMHDLPTIKEDHQTFSSSSHNTNESVGDLSPIAEEDGGSVTAALYSEHVALVSVTTVPGESPVTLISQGQVGTESPEDRINQTEVELQTKEERAVQNVAGMPPCKGAEMVEKKSSSEAEIKTDENDVVAVCRQEEVETSQTGLVDATPKSDVQVQEEVSSPVETDEKKQTSVQEKGVEEEEVKAKTVREQEAIVTSNSENRVQSACSEVLDPASDVPNTNGHMHAQDTPTTELKGQITKEEDGKGAEEKRQEDEIASSVNGSQKEHQQQADMDSSFHQQEEQACSQKVDSTVCHLSAKTESEDPSAVPSPQPPVDHRAQPVKSKAHDINPDVLSSGVLCLTGTRDKSGHALVTVTMRNTVWLNPNCNSGELVRIMVYFCSTLRKEVSALGLTVLVDARRCSPVPALFKAFNILQEAMPGCIHTVLLLADRDLALRMEKTSSIQVELLTSLKSLYKHVDVSQLPTEFEGTFPYAHSSWVSFRMRLEQLTSHCEDAVSLLQNTISKLESTVLPPTAEEAQLLLCKYRELMRTVLEDSRLVRLQLEGGAALSRLRKEETSVSLTEDYRDAIESAGRLYNQVDELVHRLVMLSNKCTQELEFIMEFKSLEEGFKEVSQWIEEVGESRLQTLSELEDSLEQLHHKQSIFREFYTAAYEHCKSGEALLKRLEKWEDVSSAELQVYEVKVRSFWVHLRDFSQRVEDTKDKIDKTVQLYEFFDKAYEWALEGMRHLACVSMEDCGMSEKCQSVITCLETYRSQHPPIPDAHFQEMKDLAGELKSEQGLKQWKFAWSKCQETKQMFEKKLEMALRSRRDSDSKSVRSDSSRRNSDVSTKHQERSSSISFSCRKAFGGAWGRDRLSSHSSTSSTPSSPSGIRLDARDTVRTPTGSPYSIEHRIPHSTPVRSHRLTRSISTDESPQSPHAEGLASTSPSGTSVQTNRRVLRKTQSFDTAGSEPVSQYGTCQRTLSEPARRGNTGVFIKGLEVSSTEVIDRPYSPRLPPIHGWSSIDAHRSGSPAPETRSKGSKLRHIVDEMVTTEREYVRSLRYIIDNYFPEMERAVLPQDLRGKRSVIFGNLEKLVDFHSQYFLKELESCCNHPLRVSHCFLRHQDQFSLYALYSKNKPKSDTLLASHGNNFFKHKQMELGDKMDLASYLLKPIQRMSKYALLLKDLIKEVSEVQEQELTYLRAAAEMVKFQLRHGNDLLAMDAIRDCDVNLKEQGQLVRQDEFTISYGRKKCQRHVFLFEDLVLFSKPKRIEGGLDVYIYKHSFKTADVGMTETSGENSLRFEIWFRRRTSKNQTYILQSSTADIKHAWTCDIARILWQQATRNKEIRMQEMVSMGVGNKPFLDIKPSDAAINDRAIDYIMKGRGARTRASIAVSLFDHSNPFKRAAVNTPVSGTPVSGGPSSSTLLGPLNLHMYSSQSLLAGERPLIGPCIEEDELEHETSSQPSMTTESSGSSSHCLSGSGSSGSDSGCVSSHLPEALSEEPSSPCDSSCYSSITSPTQEKPCFNSQYISAGDAQVIGPSTIV

Target ORF information:

RefSeq Version	XM_009303344.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303344.3

ORF Insert Sequence:

ATGGACTCTG AATCTCTGGA CAGCTGTATC CAGAGCACAC TGTCCGCTCT CTACCCTCCA 
TTTGAAGCCA CTGCGGCCAC TGTACTGTGC CAGGTGTTAG ATGTGGTGGA GACAACATAC 
CGTGGAGACG GACTGCGATA CATCATCGAC TTTCTGGTGC CCGCTAAACA CATCCTACAG 
AGCATTCAGC AGGATGCATG CTTCCCATAC TGTGGTTTTC TGTTCCGTCA TGAAGGATGG 
CCACTGTGTG TCCATGAGAA GGTCATAATC CAGCTCTCCT CTTTGGACTG GCGTGTATTA 
CGGCCCAGCG ACTTCTACTT GCAGGTGACA CCATCTGCCA AAAGCCCCCC GCGCATCAAA 
GTGAAGTGTC TGGCTGTTAC CGGGCAGCAT GCGGAGGAGC TGGACGTGCC GGAGCTAGCA 
TATACCTCTC TATTCACCAT GGACTGGCTG GACTCCATCA ACCAGGAGAG GAGTGTGGTC 
AGAAGAGCTG CCCTGGAACA CTGTCTTTTA TCAGCGGATA ATGATATTTT TAGGGTACCG 
TGGGAGGATA TTGTTCACCC GGAATTTATC AGCAGACCCT CTAAAGTTGC AGAACAAAGT 
GAAAGTAGAA GGGTTGCCAA AGAAGTTGCA AATGGAGACG GGCGAGACTC TGATGTAGAA 
CAAGACAACA ATCCTGAGGA CATACAATGT AGAATTTTTT ATGACACTTC CACGAACCAT 
TCAGGGGATA TGGATTGTCA GAGCAAGGAA GTTAAGCTCC TCCCTGTTCT TAGTGAGATT 
AATAAAGATA TCAGTGGGAG GAGCTCTAGC AGCACTGCGG ACGATTCAGA GGGCGAATAT 
GTGGAGCTAG CTGATATCTC CTTACCACGC TTTTCCCCAC AAAAGGGCTC TCTTACTCAA 
GCCATTAGCA TGAACTATAG AAATCTACAT AAACCTCAGA CTCCTGTTTC CACTCAGCTT 
AAAACCAAAC CAGATGAGAG TCTTTTGAAG AATGGTGCAT GCTCTCAAAG CATGGTGTGT 
GCCATGATGA TCGAGGAAAG CCTGAGTGAT ACCTACCAAA CAGTGATCCT TACTCCCACT 
GCTCCTGCAG AGGCAGAGCG TCCGCTCCAG CAGGTGGACA TGTCTGAACC CGCCTGTGCA 
GCAGGTACAG CTTCCTTTGA CAGTGTGTCT GAGCATGAGT CTGAGAGAGA GCTATTATCT 
CAACACTCTG ACTGTGAGGA TGCTTCACTA TCTGATATAT CAACAATTAA CATATCGCAA 
ACAGACATTA AACCAATCAA CACATCACAA CTAGACAACA ATGTAGTCTG CAAATCACAA 
GACAGCGAAC CATACAGTAC ATCACAATTA GACAAGAATC TAGTGAACAC ATCACAAACA 
GATACTGAAT CATTGAGCAT GTCTCAATTA GACGATAATT TGGACTGCAC ATCACATCCA 
GATGTTAACC AGAAACCATC AAGTATGTCT CAACTAGAAC TTGAACCACT AACCGTATCT 
CAGCTTGACA GCAAGCTAAT CAGCACATCA CAACCTGATA GCACATCACT AGATGTAGAA 
CCAGTTAGCA CATCTAATGT AGACAACAAA CCAGTCAGCA CATCACAACC GGACAGCACA 
TTACAGTTGG TCAATGAACC AGTCAAGACA TCACAAGATG GTGAACCAGT AAGCAGATTT 
CATGTAGACC ACAAACCAGT AAGCACATTA CAACCAGACA GCCAACCAGT CATCACATCA 
GTCAATGAGC CATTTAGGAC ATCACAACCA GACAATAAAT CAGTAAGCAC AACTCAACTA 
GACAGCAAAC CAGTTAGTGT ATCACAACAC AACAAACAAG AAAGCAAATC TGTCAACACA 
TCTGTTGACA ATGAGCCAGT CAAGACATCA CAGCCAGACA CTGAATCACT AAGAATATCT 
CAATTTGACA GCAAACCAGT CAGCAACTCA CAACTAGGCA GCAAACAAGT CAGCATTAAA 
CAACTAGTCA ATGAGATATT TAGTACACCA CAACCAGACA ATAAATCAGT GAGCACAACT 
CAACTAGAAA GAAAACTAGT TTGCGGATCA CAAGACAACA AACAAGAAAG CAAATCTGTC 
AACACATTAG TAGACAGTGA GCCAGTCAAG ACATCACAGA CAGACACAGA ACTGCTAAGA 
ATATCACAGT TTGATAGCAA ACCAGTCAGC AACTCACAAC TAGATGGCAA ACAAGTCAGC 
ATTTCACAGC TAGTCAATGA GATATTTAGT ACACCACAAC CAGACAATAA ATCAGTAAGC 
CCAACTCAAC TAGACAGTAA GCTAGTTAGC ACATTACAAG ACAAACAAGA AAGCAAATCT 
GTCAATAATT CAATAGACAA TGAGCCAGTC AAGACATCAC AGGCAGACAC AGAACCGCTA 
AAAAGGTCTC AGTTTGACAG CAACCCAGTC AGCAACTCAC AGCTAGATGG CAAACAAGTC 
AGCATTTCAC AACTAGTCAA TGAGATATTT AGTACACCAC AACCAGACAA TAAATCAGTG 
AGCACAACTC AACTAGATGG CAACATAGAT AGAACATCAC AATTAGACAG CAAACAAGAA 
AGTGTCTCTC AAACAGACAG CAAACCAGTC AGCATATCAC CAGACAATGA GCCAGTGAAG 
ACATCACAAC CACAACTAGA AAGCACATTA CACCCAAACA GCAAACAAGC AAGCATGTCA 
CAACTAGTCA ATGAACACAT CCAGACCTCA CAGCCAGACC TAAAACCAGT AAGCATTGCT 
CAACTCTACA GTGAAGATGC TAAACCAGAT GGATACGCTG ATGTCAAACC AATAACCACT 
GAGCTAGACA ATGGACTAGT GAGCACATCA CAACCAGATA ACCAACCAGT TGACACACCA 
CAAGCAGAAT GTACAACAGT TAACATAACC TTACCAGAGG GCAACCCCAA TGACACATCA 
TATATTGTCA ACCTGCCGCT AAGTACATCA CAACCAAACA GCAAAACAGT TCATGTGGAG 
CAAGCAGACA TAAATGTAAT TAATACATCA CAGCCAGAGA CTGAAATGTT ATCTTGTGTT 
TTGGAAAGGG AGAACTCAGA ACAGGTTTCT CCAGCTTATA TTGAACTCAT TGTGTCAGAT 
CCTTATGCAG AAGTCAGATG TGAATCTAAA GAGCTGAGAG TTAATTGTGA TCTTGAAGGC 
AGTTGTGGTT CACAGAACAA CAGAGCGTCA CTTGCCGAGA CAGTAGCTGA GAGTGTGTCT 
TCGCAAAATC AGATTGATCA AACAGGCAAT AGCCTGTATC AGATACACAA ACCCATCCAT 
ACACCATCTG AGCAAATGCA TGATCTTCCT ACCATAAAAG AAGACCATCA GACATTCAGT 
AGCAGTTCTC ATAACACGAA TGAATCTGTT GGTGATTTAA GTCCCATTGC AGAAGAAGAT 
GGTGGGTCTG TTACAGCTGC TCTGTATAGT GAGCATGTAG CCTTAGTTTC TGTCACCACA 
GTGCCTGGGG AGAGTCCAGT TACACTCATT TCACAGGGTC AGGTGGGGAC AGAGTCTCCT 
GAGGACAGAA TCAACCAGAC AGAGGTGGAA CTTCAGACAA AGGAAGAACG TGCAGTACAA 
AACGTAGCTG GAATGCCACC TTGTAAGGGG GCTGAAATGG TAGAAAAGAA AAGCAGTAGC 
GAGGCAGAAA TCAAAACTGA TGAGAATGAT GTAGTGGCAG TGTGTAGGCA AGAGGAAGTT 
GAGACTTCAC AAACAGGTCT AGTAGATGCT ACGCCCAAGT CTGACGTGCA AGTTCAGGAG 
GAGGTCAGCT CCCCTGTAGA AACAGATGAG AAGAAACAAA CCAGTGTTCA AGAAAAAGGG 
GTGGAGGAAG AGGAGGTCAA GGCCAAAACT GTGAGGGAGC AAGAGGCCAT AGTGACCAGC 
AATTCTGAAA ATAGAGTCCA GTCAGCATGC TCTGAAGTGC TTGATCCAGC CTCTGATGTC 
CCGAACACAA ATGGACACAT GCATGCTCAG GACACTCCTA CCACTGAACT AAAAGGGCAA 
ATAACAAAGG AGGAGGATGG AAAAGGGGCA GAGGAGAAAA GGCAAGAGGA TGAGATTGCT 
TCTTCTGTCA ATGGCTCTCA AAAAGAGCAT CAGCAGCAAG CAGACATGGA CTCCAGCTTC 
CATCAGCAGG AAGAGCAAGC ATGCAGTCAG AAAGTAGACA GCACTGTTTG CCACCTGTCT 
GCTAAAACTG AGAGTGAAGA CCCCTCAGCA GTTCCTTCAC CACAGCCACC AGTCGATCAT 
AGAGCACAAC CAGTCAAGAG CAAGGCCCAT GACATAAATC CTGATGTCCT AAGCTCAGGT 
GTGCTCTGCC TGACTGGAAC AAGAGATAAA TCAGGCCATG CCTTGGTCAC TGTGACAATG 
AGAAACACTG TCTGGCTGAA TCCAAACTGC AACAGTGGAG AGCTAGTGCG AATCATGGTG 
TATTTCTGCA GTACACTCAG GAAGGAAGTA AGTGCTTTGG GATTGACTGT CCTGGTGGAT 
GCCCGCAGGT GTTCACCTGT TCCCGCCCTT TTCAAAGCCT TCAACATCCT CCAGGAAGCC 
ATGCCGGGAT GCATTCATAC TGTACTGCTG CTGGCCGACA GAGATCTGGC TCTACGCATG 
GAGAAAACCT CTTCTATACA GGTGGAGCTG CTGACATCAC TCAAGTCTCT CTATAAACAT 
GTGGACGTTT CTCAGCTTCC CACTGAGTTT GAAGGCACTT TCCCTTATGC ACACAGCAGC 
TGGGTCAGCT TTAGAATGAG GTTGGAGCAG TTGACCAGTC ATTGTGAAGA TGCTGTGAGC 
CTGCTTCAGA ACACCATAAG CAAACTGGAG TCCACAGTGC TGCCCCCTAC AGCAGAGGAG 
GCTCAACTCT TACTGTGTAA GTACAGAGAG CTGATGAGGA CGGTTCTGGA GGACAGCAGG 
CTGGTGCGAC TACAGTTGGA GGGAGGAGCA GCTCTGTCCC GTTTACGAAA AGAAGAGACC 
AGCGTTAGTC TCACAGAGGA CTACAGAGAT GCCATTGAAA GTGCTGGCCG TTTGTATAAC 
CAAGTGGACG AGCTGGTTCA CAGACTGGTG ATGCTGTCCA ATAAATGCAC ACAGGAACTG 
GAATTCATCA TGGAGTTTAA GAGCCTGGAG GAGGGATTCA AAGAGGTTAG TCAGTGGATA 
GAAGAGGTGG GAGAGTCTCG TCTGCAGACG CTGAGCGAGC TGGAGGATTC CTTGGAGCAG 
TTACATCATA AGCAGAGCAT CTTCAGAGAA TTTTACACAG CTGCATATGA ACACTGTAAG 
AGTGGTGAAG CTCTCCTCAA GCGTTTAGAG AAGTGGGAAG ACGTCTCCTC TGCAGAGCTG 
CAAGTGTATG AGGTGAAAGT ACGTTCATTC TGGGTGCATC TGCGGGATTT CTCTCAGCGA 
GTGGAGGACA CCAAGGATAA AATAGACAAG ACTGTTCAAC TCTATGAGTT CTTTGATAAG 
GCATATGAAT GGGCTCTAGA GGGCATGCGT CATTTGGCGT GTGTCAGTAT GGAGGATTGT 
GGCATGTCTG AAAAGTGTCA GAGCGTGATC ACGTGTTTGG AGACTTACCG CAGTCAGCAC 
CCCCCGATCC CTGATGCACA CTTCCAGGAG ATGAAGGACC TCGCTGGGGA ACTGAAAAGC 
GAACAGGGCC TCAAGCAGTG GAAGTTTGCC TGGTCCAAGT GTCAGGAGAC CAAGCAGATG 
TTTGAAAAGA AGCTGGAGAT GGCCCTGCGT TCCCGCCGTG ACAGCGACTC CAAGTCAGTA 
AGGAGTGACT CTTCTCGTCG TAACTCAGAT GTTAGCACTA AACACCAAGA GCGTAGCAGT 
AGCATCTCCT TCTCATGTCG GAAAGCGTTC GGCGGAGCCT GGGGTCGAGA TAGACTTTCC 
TCACATTCTA GCACATCCTC AACTCCCAGT AGCCCTTCAG GCATTCGTCT GGATGCCCGT 
GACACTGTCC GAACTCCAAC AGGAAGTCCA TACAGCATTG AGCACAGAAT CCCCCACAGC 
ACCCCTGTCA GATCCCACAG GCTAACACGT AGCATCTCCA CAGATGAGTC TCCTCAGAGC 
CCACATGCAG AGGGGCTAGC GTCCACAAGC CCAAGCGGCA CTTCTGTTCA AACAAACCGC 
AGGGTTTTGC GGAAGACCCA GAGCTTCGAT ACAGCTGGCA GTGAGCCTGT GTCACAGTAC 
GGGACCTGTC AGAGGACTTT GAGTGAGCCT GCACGCAGAG GAAACACAGG GGTGTTCATT 
AAGGGTCTGG AGGTGAGCAG CACTGAAGTG ATTGATAGGC CGTACAGTCC ACGACTACCA 
CCCATCCATG GATGGTCTTC AATTGATGCA CACCGCAGCG GGAGTCCTGC TCCAGAAACG 
CGCAGCAAGG GCAGCAAGTT ACGACACATT GTAGATGAGA TGGTGACAAC AGAGCGGGAA 
TATGTGCGTT CCCTGCGGTA TATCATTGAC AACTATTTCC CTGAGATGGA GCGCGCTGTC 
CTGCCGCAGG ATCTGCGGGG AAAGCGGAGC GTCATATTTG GAAATCTGGA GAAACTGGTG 
GACTTTCATA GTCAGTATTT CCTAAAAGAG CTGGAGAGCT GCTGTAATCA TCCTCTACGC 
GTCAGCCACT GTTTCCTACG ACATCAAGAC CAGTTTAGCC TCTATGCATT ATACAGCAAG 
AACAAGCCAA AATCAGACAC ACTCCTGGCC AGCCATGGAA ACAACTTCTT CAAGCATAAG 
CAGATGGAGC TGGGAGATAA GATGGACCTG GCATCGTACC TGCTGAAGCC CATCCAGCGC 
ATGAGTAAAT ACGCTCTTCT GCTGAAAGAC CTGATCAAAG AAGTGAGCGA GGTCCAGGAG 
CAGGAACTTA CATACCTGCG AGCCGCAGCA GAGATGGTCA AGTTTCAGCT CCGCCATGGC 
AATGATCTAC TCGCCATGGA CGCTATCAGA GATTGTGATG TGAACCTAAA AGAGCAAGGA 
CAGTTGGTTC GTCAAGATGA GTTCACGATT TCATATGGCA GAAAGAAGTG CCAGAGACAC 
GTGTTCCTAT TTGAGGACCT AGTGCTCTTC AGCAAACCCA AGCGCATCGA GGGTGGACTA 
GATGTTTACA TATACAAGCA TTCTTTTAAG ACGGCTGATG TGGGTATGAC AGAGACGTCA 
GGTGAGAACA GTCTGAGGTT TGAAATTTGG TTTCGGAGGA GAACTTCAAA GAACCAGACC 
TACATTCTCC AGTCCAGCAC TGCTGACATC AAACATGCCT GGACTTGCGA CATCGCACGA 
ATACTGTGGC AGCAGGCCAC GCGAAACAAA GAAATTCGAA TGCAGGAGAT GGTCTCAATG 
GGTGTGGGCA ATAAACCCTT CCTGGACATT AAACCCAGTG ATGCTGCAAT CAACGACAGA 
GCCATCGACT ACATTATGAA GGGCAGAGGA GCTAGAACGA GAGCCTCCAT TGCTGTTTCT 
CTGTTTGACC ACTCAAACCC ATTCAAAAGA GCTGCAGTAA ACACCCCCGT CAGCGGGACC 
CCGGTGTCTG GCGGTCCTTC CTCATCCACC CTGCTGGGGC CCTTGAATCT GCACATGTAC 
AGCAGCCAAA GTCTGTTGGC GGGGGAGAGA CCTCTGATTG GCCCCTGCAT CGAGGAGGAC 
GAGCTGGAGC ATGAGACCAG TAGTCAGCCC TCTATGACTA CAGAGAGTTC GGGTTCGTCG 
TCTCACTGTC TGTCTGGCAG TGGCTCTAGC GGATCAGACA GCGGCTGTGT GTCCAGTCAC 
CTTCCTGAGG CTTTGTCTGA GGAGCCCAGC TCACCTTGTG ACTCCTCGTG TTATTCCTCC 
ATCACCTCTC CCACGCAGGA GAAGCCCTGC TTTAACAGCC AGTATATCTC AGCGGGGGAT 
GCTCAGGTGA TCGGACCCTC TACTATAGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg4 ( XM_009303344.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301619.1 Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X1, mRNA.

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CloneID	ODa39866
Clone ID Related Accession (Same CDS sequence)	XM_009303344.3 , XM_009303344.2
Accession Version	XM_009303344.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein plekhg4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303344.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTG AATCTCTGGA CAGCTGTATC CAGAGCACAC TGTCCGCTCT CTACCCTCCA 
TTTGAAGCCA CTGCGGCCAC TGTACTGTGC CAGGTGTTAG ATGTGGTGGA GACAACATAC 
CGTGGAGACG GACTGCGATA CATCATCGAC TTTCTGGTGC CCGCTAAACA CATCCTACAG 
AGCATTCAGC AGGATGCATG CTTCCCATAC TGTGGTTTTC TGTTCCGTCA TGAAGGATGG 
CCACTGTGTG TCCATGAGAA GGTCATAATC CAGCTCTCCT CTTTGGACTG GCGTGTATTA 
CGGCCCAGCG ACTTCTACTT GCAGGTGACA CCATCTGCCA AAAGCCCCCC GCGCATCAAA 
GTGAAGTGTC TGGCTGTTAC CGGGCAGCAT GCGGAGGAGC TGGACGTGCC GGAGCTAGCA 
TATACCTCTC TATTCACCAT GGACTGGCTG GACTCCATCA ACCAGGAGAG GAGTGTGGTC 
AGAAGAGCTG CCCTGGAACA CTGTCTTTTA TCAGCGGATA ATGATATTTT TAGGGTACCG 
TGGGAGGATA TTGTTCACCC GGAATTTATC AGCAGACCCT CTAAAGTTGC AGAACAAAGT 
GAAAGTAGAA GGGTTGCCAA AGAAGTTGCA AATGGAGACG GGCGAGACTC TGATGTAGAA 
CAAGACAACA ATCCTGAGGA CATACAATGT AGAATTTTTT ATGACACTTC CACGAACCAT 
TCAGGGGATA TGGATTGTCA GAGCAAGGAA GTTAAGCTCC TCCCTGTTCT TAGTGAGATT 
AATAAAGATA TCAGTGGGAG GAGCTCTAGC AGCACTGCGG ACGATTCAGA GGGCGAATAT 
GTGGAGCTAG CTGATATCTC CTTACCACGC TTTTCCCCAC AAAAGGGCTC TCTTACTCAA 
GCCATTAGCA TGAACTATAG AAATCTACAT AAACCTCAGA CTCCTGTTTC CACTCAGCTT 
AAAACCAAAC CAGATGAGAG TCTTTTGAAG AATGGTGCAT GCTCTCAAAG CATGGTGTGT 
GCCATGATGA TCGAGGAAAG CCTGAGTGAT ACCTACCAAA CAGTGATCCT TACTCCCACT 
GCTCCTGCAG AGGCAGAGCG TCCGCTCCAG CAGGTGGACA TGTCTGAACC CGCCTGTGCA 
GCAGGTACAG CTTCCTTTGA CAGTGTGTCT GAGCATGAGT CTGAGAGAGA GCTATTATCT 
CAACACTCTG ACTGTGAGGA TGCTTCACTA TCTGATATAT CAACAATTAA CATATCGCAA 
ACAGACATTA AACCAATCAA CACATCACAA CTAGACAACA ATGTAGTCTG CAAATCACAA 
GACAGCGAAC CATACAGTAC ATCACAATTA GACAAGAATC TAGTGAACAC ATCACAAACA 
GATACTGAAT CATTGAGCAT GTCTCAATTA GACGATAATT TGGACTGCAC ATCACATCCA 
GATGTTAACC AGAAACCATC AAGTATGTCT CAACTAGAAC TTGAACCACT AACCGTATCT 
CAGCTTGACA GCAAGCTAAT CAGCACATCA CAACCTGATA GCACATCACT AGATGTAGAA 
CCAGTTAGCA CATCTAATGT AGACAACAAA CCAGTCAGCA CATCACAACC GGACAGCACA 
TTACAGTTGG TCAATGAACC AGTCAAGACA TCACAAGATG GTGAACCAGT AAGCAGATTT 
CATGTAGACC ACAAACCAGT AAGCACATTA CAACCAGACA GCCAACCAGT CATCACATCA 
GTCAATGAGC CATTTAGGAC ATCACAACCA GACAATAAAT CAGTAAGCAC AACTCAACTA 
GACAGCAAAC CAGTTAGTGT ATCACAACAC AACAAACAAG AAAGCAAATC TGTCAACACA 
TCTGTTGACA ATGAGCCAGT CAAGACATCA CAGCCAGACA CTGAATCACT AAGAATATCT 
CAATTTGACA GCAAACCAGT CAGCAACTCA CAACTAGGCA GCAAACAAGT CAGCATTAAA 
CAACTAGTCA ATGAGATATT TAGTACACCA CAACCAGACA ATAAATCAGT GAGCACAACT 
CAACTAGAAA GAAAACTAGT TTGCGGATCA CAAGACAACA AACAAGAAAG CAAATCTGTC 
AACACATTAG TAGACAGTGA GCCAGTCAAG ACATCACAGA CAGACACAGA ACTGCTAAGA 
ATATCACAGT TTGATAGCAA ACCAGTCAGC AACTCACAAC TAGATGGCAA ACAAGTCAGC 
ATTTCACAGC TAGTCAATGA GATATTTAGT ACACCACAAC CAGACAATAA ATCAGTAAGC 
CCAACTCAAC TAGACAGTAA GCTAGTTAGC ACATTACAAG ACAAACAAGA AAGCAAATCT 
GTCAATAATT CAATAGACAA TGAGCCAGTC AAGACATCAC AGGCAGACAC AGAACCGCTA 
AAAAGGTCTC AGTTTGACAG CAACCCAGTC AGCAACTCAC AGCTAGATGG CAAACAAGTC 
AGCATTTCAC AACTAGTCAA TGAGATATTT AGTACACCAC AACCAGACAA TAAATCAGTG 
AGCACAACTC AACTAGATGG CAACATAGAT AGAACATCAC AATTAGACAG CAAACAAGAA 
AGTGTCTCTC AAACAGACAG CAAACCAGTC AGCATATCAC CAGACAATGA GCCAGTGAAG 
ACATCACAAC CACAACTAGA AAGCACATTA CACCCAAACA GCAAACAAGC AAGCATGTCA 
CAACTAGTCA ATGAACACAT CCAGACCTCA CAGCCAGACC TAAAACCAGT AAGCATTGCT 
CAACTCTACA GTGAAGATGC TAAACCAGAT GGATACGCTG ATGTCAAACC AATAACCACT 
GAGCTAGACA ATGGACTAGT GAGCACATCA CAACCAGATA ACCAACCAGT TGACACACCA 
CAAGCAGAAT GTACAACAGT TAACATAACC TTACCAGAGG GCAACCCCAA TGACACATCA 
TATATTGTCA ACCTGCCGCT AAGTACATCA CAACCAAACA GCAAAACAGT TCATGTGGAG 
CAAGCAGACA TAAATGTAAT TAATACATCA CAGCCAGAGA CTGAAATGTT ATCTTGTGTT 
TTGGAAAGGG AGAACTCAGA ACAGGTTTCT CCAGCTTATA TTGAACTCAT TGTGTCAGAT 
CCTTATGCAG AAGTCAGATG TGAATCTAAA GAGCTGAGAG TTAATTGTGA TCTTGAAGGC 
AGTTGTGGTT CACAGAACAA CAGAGCGTCA CTTGCCGAGA CAGTAGCTGA GAGTGTGTCT 
TCGCAAAATC AGATTGATCA AACAGGCAAT AGCCTGTATC AGATACACAA ACCCATCCAT 
ACACCATCTG AGCAAATGCA TGATCTTCCT ACCATAAAAG AAGACCATCA GACATTCAGT 
AGCAGTTCTC ATAACACGAA TGAATCTGTT GGTGATTTAA GTCCCATTGC AGAAGAAGAT 
GGTGGGTCTG TTACAGCTGC TCTGTATAGT GAGCATGTAG CCTTAGTTTC TGTCACCACA 
GTGCCTGGGG AGAGTCCAGT TACACTCATT TCACAGGGTC AGGTGGGGAC AGAGTCTCCT 
GAGGACAGAA TCAACCAGAC AGAGGTGGAA CTTCAGACAA AGGAAGAACG TGCAGTACAA 
AACGTAGCTG GAATGCCACC TTGTAAGGGG GCTGAAATGG TAGAAAAGAA AAGCAGTAGC 
GAGGCAGAAA TCAAAACTGA TGAGAATGAT GTAGTGGCAG TGTGTAGGCA AGAGGAAGTT 
GAGACTTCAC AAACAGGTCT AGTAGATGCT ACGCCCAAGT CTGACGTGCA AGTTCAGGAG 
GAGGTCAGCT CCCCTGTAGA AACAGATGAG AAGAAACAAA CCAGTGTTCA AGAAAAAGGG 
GTGGAGGAAG AGGAGGTCAA GGCCAAAACT GTGAGGGAGC AAGAGGCCAT AGTGACCAGC 
AATTCTGAAA ATAGAGTCCA GTCAGCATGC TCTGAAGTGC TTGATCCAGC CTCTGATGTC 
CCGAACACAA ATGGACACAT GCATGCTCAG GACACTCCTA CCACTGAACT AAAAGGGCAA 
ATAACAAAGG AGGAGGATGG AAAAGGGGCA GAGGAGAAAA GGCAAGAGGA TGAGATTGCT 
TCTTCTGTCA ATGGCTCTCA AAAAGAGCAT CAGCAGCAAG CAGACATGGA CTCCAGCTTC 
CATCAGCAGG AAGAGCAAGC ATGCAGTCAG AAAGTAGACA GCACTGTTTG CCACCTGTCT 
GCTAAAACTG AGAGTGAAGA CCCCTCAGCA GTTCCTTCAC CACAGCCACC AGTCGATCAT 
AGAGCACAAC CAGTCAAGAG CAAGGCCCAT GACATAAATC CTGATGTCCT AAGCTCAGGT 
GTGCTCTGCC TGACTGGAAC AAGAGATAAA TCAGGCCATG CCTTGGTCAC TGTGACAATG 
AGAAACACTG TCTGGCTGAA TCCAAACTGC AACAGTGGAG AGCTAGTGCG AATCATGGTG 
TATTTCTGCA GTACACTCAG GAAGGAAGTA AGTGCTTTGG GATTGACTGT CCTGGTGGAT 
GCCCGCAGGT GTTCACCTGT TCCCGCCCTT TTCAAAGCCT TCAACATCCT CCAGGAAGCC 
ATGCCGGGAT GCATTCATAC TGTACTGCTG CTGGCCGACA GAGATCTGGC TCTACGCATG 
GAGAAAACCT CTTCTATACA GGTGGAGCTG CTGACATCAC TCAAGTCTCT CTATAAACAT 
GTGGACGTTT CTCAGCTTCC CACTGAGTTT GAAGGCACTT TCCCTTATGC ACACAGCAGC 
TGGGTCAGCT TTAGAATGAG GTTGGAGCAG TTGACCAGTC ATTGTGAAGA TGCTGTGAGC 
CTGCTTCAGA ACACCATAAG CAAACTGGAG TCCACAGTGC TGCCCCCTAC AGCAGAGGAG 
GCTCAACTCT TACTGTGTAA GTACAGAGAG CTGATGAGGA CGGTTCTGGA GGACAGCAGG 
CTGGTGCGAC TACAGTTGGA GGGAGGAGCA GCTCTGTCCC GTTTACGAAA AGAAGAGACC 
AGCGTTAGTC TCACAGAGGA CTACAGAGAT GCCATTGAAA GTGCTGGCCG TTTGTATAAC 
CAAGTGGACG AGCTGGTTCA CAGACTGGTG ATGCTGTCCA ATAAATGCAC ACAGGAACTG 
GAATTCATCA TGGAGTTTAA GAGCCTGGAG GAGGGATTCA AAGAGGTTAG TCAGTGGATA 
GAAGAGGTGG GAGAGTCTCG TCTGCAGACG CTGAGCGAGC TGGAGGATTC CTTGGAGCAG 
TTACATCATA AGCAGAGCAT CTTCAGAGAA TTTTACACAG CTGCATATGA ACACTGTAAG 
AGTGGTGAAG CTCTCCTCAA GCGTTTAGAG AAGTGGGAAG ACGTCTCCTC TGCAGAGCTG 
CAAGTGTATG AGGTGAAAGT ACGTTCATTC TGGGTGCATC TGCGGGATTT CTCTCAGCGA 
GTGGAGGACA CCAAGGATAA AATAGACAAG ACTGTTCAAC TCTATGAGTT CTTTGATAAG 
GCATATGAAT GGGCTCTAGA GGGCATGCGT CATTTGGCGT GTGTCAGTAT GGAGGATTGT 
GGCATGTCTG AAAAGTGTCA GAGCGTGATC ACGTGTTTGG AGACTTACCG CAGTCAGCAC 
CCCCCGATCC CTGATGCACA CTTCCAGGAG ATGAAGGACC TCGCTGGGGA ACTGAAAAGC 
GAACAGGGCC TCAAGCAGTG GAAGTTTGCC TGGTCCAAGT GTCAGGAGAC CAAGCAGATG 
TTTGAAAAGA AGCTGGAGAT GGCCCTGCGT TCCCGCCGTG ACAGCGACTC CAAGTCAGTA 
AGGAGTGACT CTTCTCGTCG TAACTCAGAT GTTAGCACTA AACACCAAGA GCGTAGCAGT 
AGCATCTCCT TCTCATGTCG GAAAGCGTTC GGCGGAGCCT GGGGTCGAGA TAGACTTTCC 
TCACATTCTA GCACATCCTC AACTCCCAGT AGCCCTTCAG GCATTCGTCT GGATGCCCGT 
GACACTGTCC GAACTCCAAC AGGAAGTCCA TACAGCATTG AGCACAGAAT CCCCCACAGC 
ACCCCTGTCA GATCCCACAG GCTAACACGT AGCATCTCCA CAGATGAGTC TCCTCAGAGC 
CCACATGCAG AGGGGCTAGC GTCCACAAGC CCAAGCGGCA CTTCTGTTCA AACAAACCGC 
AGGGTTTTGC GGAAGACCCA GAGCTTCGAT ACAGCTGGCA GTGAGCCTGT GTCACAGTAC 
GGGACCTGTC AGAGGACTTT GAGTGAGCCT GCACGCAGAG GAAACACAGG GGTGTTCATT 
AAGGGTCTGG AGGTGAGCAG CACTGAAGTG ATTGATAGGC CGTACAGTCC ACGACTACCA 
CCCATCCATG GATGGTCTTC AATTGATGCA CACCGCAGCG GGAGTCCTGC TCCAGAAACG 
CGCAGCAAGG GCAGCAAGTT ACGACACATT GTAGATGAGA TGGTGACAAC AGAGCGGGAA 
TATGTGCGTT CCCTGCGGTA TATCATTGAC AACTATTTCC CTGAGATGGA GCGCGCTGTC 
CTGCCGCAGG ATCTGCGGGG AAAGCGGAGC GTCATATTTG GAAATCTGGA GAAACTGGTG 
GACTTTCATA GTCAGTATTT CCTAAAAGAG CTGGAGAGCT GCTGTAATCA TCCTCTACGC 
GTCAGCCACT GTTTCCTACG ACATCAAGAC CAGTTTAGCC TCTATGCATT ATACAGCAAG 
AACAAGCCAA AATCAGACAC ACTCCTGGCC AGCCATGGAA ACAACTTCTT CAAGCATAAG 
CAGATGGAGC TGGGAGATAA GATGGACCTG GCATCGTACC TGCTGAAGCC CATCCAGCGC 
ATGAGTAAAT ACGCTCTTCT GCTGAAAGAC CTGATCAAAG AAGTGAGCGA GGTCCAGGAG 
CAGGAACTTA CATACCTGCG AGCCGCAGCA GAGATGGTCA AGTTTCAGCT CCGCCATGGC 
AATGATCTAC TCGCCATGGA CGCTATCAGA GATTGTGATG TGAACCTAAA AGAGCAAGGA 
CAGTTGGTTC GTCAAGATGA GTTCACGATT TCATATGGCA GAAAGAAGTG CCAGAGACAC 
GTGTTCCTAT TTGAGGACCT AGTGCTCTTC AGCAAACCCA AGCGCATCGA GGGTGGACTA 
GATGTTTACA TATACAAGCA TTCTTTTAAG ACGGCTGATG TGGGTATGAC AGAGACGTCA 
GGTGAGAACA GTCTGAGGTT TGAAATTTGG TTTCGGAGGA GAACTTCAAA GAACCAGACC 
TACATTCTCC AGTCCAGCAC TGCTGACATC AAACATGCCT GGACTTGCGA CATCGCACGA 
ATACTGTGGC AGCAGGCCAC GCGAAACAAA GAAATTCGAA TGCAGGAGAT GGTCTCAATG 
GGTGTGGGCA ATAAACCCTT CCTGGACATT AAACCCAGTG ATGCTGCAAT CAACGACAGA 
GCCATCGACT ACATTATGAA GGGCAGAGGA GCTAGAACGA GAGCCTCCAT TGCTGTTTCT 
CTGTTTGACC ACTCAAACCC ATTCAAAAGA GCTGCAGTAA ACACCCCCGT CAGCGGGACC 
CCGGTGTCTG GCGGTCCTTC CTCATCCACC CTGCTGGGGC CCTTGAATCT GCACATGTAC 
AGCAGCCAAA GTCTGTTGGC GGGGGAGAGA CCTCTGATTG GCCCCTGCAT CGAGGAGGAC 
GAGCTGGAGC ATGAGACCAG TAGTCAGCCC TCTATGACTA CAGAGAGTTC GGGTTCGTCG 
TCTCACTGTC TGTCTGGCAG TGGCTCTAGC GGATCAGACA GCGGCTGTGT GTCCAGTCAC 
CTTCCTGAGG CTTTGTCTGA GGAGCCCAGC TCACCTTGTG ACTCCTCGTG TTATTCCTCC 
ATCACCTCTC CCACGCAGGA GAAGCCCTGC TTTAACAGCC AGTATATCTC AGCGGGGGAT 
GCTCAGGTGA TCGGACCCTC TACTATAGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301619.1
CDS	167..8059
Translation	MDSESLDSCIQSTLSALYPPFEATAATVLCQVLDVVETTYRGDGLRYIIDFLVPAKHILQSIQQDACFPYCGFLFRHEGWPLCVHEKVIIQLSSLDWRVLRPSDFYLQVTPSAKSPPRIKVKCLAVTGQHAEELDVPELAYTSLFTMDWLDSINQERSVVRRAALEHCLLSADNDIFRVPWEDIVHPEFISRPSKVAEQSESRRVAKEVANGDGRDSDVEQDNNPEDIQCRIFYDTSTNHSGDMDCQSKEVKLLPVLSEINKDISGRSSSSTADDSEGEYVELADISLPRFSPQKGSLTQAISMNYRNLHKPQTPVSTQLKTKPDESLLKNGACSQSMVCAMMIEESLSDTYQTVILTPTAPAEAERPLQQVDMSEPACAAGTASFDSVSEHESERELLSQHSDCEDASLSDISTINISQTDIKPINTSQLDNNVVCKSQDSEPYSTSQLDKNLVNTSQTDTESLSMSQLDDNLDCTSHPDVNQKPSSMSQLELEPLTVSQLDSKLISTSQPDSTSLDVEPVSTSNVDNKPVSTSQPDSTLQLVNEPVKTSQDGEPVSRFHVDHKPVSTLQPDSQPVITSVNEPFRTSQPDNKSVSTTQLDSKPVSVSQHNKQESKSVNTSVDNEPVKTSQPDTESLRISQFDSKPVSNSQLGSKQVSIKQLVNEIFSTPQPDNKSVSTTQLERKLVCGSQDNKQESKSVNTLVDSEPVKTSQTDTELLRISQFDSKPVSNSQLDGKQVSISQLVNEIFSTPQPDNKSVSPTQLDSKLVSTLQDKQESKSVNNSIDNEPVKTSQADTEPLKRSQFDSNPVSNSQLDGKQVSISQLVNEIFSTPQPDNKSVSTTQLDGNIDRTSQLDSKQESVSQTDSKPVSISPDNEPVKTSQPQLESTLHPNSKQASMSQLVNEHIQTSQPDLKPVSIAQLYSEDAKPDGYADVKPITTELDNGLVSTSQPDNQPVDTPQAECTTVNITLPEGNPNDTSYIVNLPLSTSQPNSKTVHVEQADINVINTSQPETEMLSCVLERENSEQVSPAYIELIVSDPYAEVRCESKELRVNCDLEGSCGSQNNRASLAETVAESVSSQNQIDQTGNSLYQIHKPIHTPSEQMHDLPTIKEDHQTFSSSSHNTNESVGDLSPIAEEDGGSVTAALYSEHVALVSVTTVPGESPVTLISQGQVGTESPEDRINQTEVELQTKEERAVQNVAGMPPCKGAEMVEKKSSSEAEIKTDENDVVAVCRQEEVETSQTGLVDATPKSDVQVQEEVSSPVETDEKKQTSVQEKGVEEEEVKAKTVREQEAIVTSNSENRVQSACSEVLDPASDVPNTNGHMHAQDTPTTELKGQITKEEDGKGAEEKRQEDEIASSVNGSQKEHQQQADMDSSFHQQEEQACSQKVDSTVCHLSAKTESEDPSAVPSPQPPVDHRAQPVKSKAHDINPDVLSSGVLCLTGTRDKSGHALVTVTMRNTVWLNPNCNSGELVRIMVYFCSTLRKEVSALGLTVLVDARRCSPVPALFKAFNILQEAMPGCIHTVLLLADRDLALRMEKTSSIQVELLTSLKSLYKHVDVSQLPTEFEGTFPYAHSSWVSFRMRLEQLTSHCEDAVSLLQNTISKLESTVLPPTAEEAQLLLCKYRELMRTVLEDSRLVRLQLEGGAALSRLRKEETSVSLTEDYRDAIESAGRLYNQVDELVHRLVMLSNKCTQELEFIMEFKSLEEGFKEVSQWIEEVGESRLQTLSELEDSLEQLHHKQSIFREFYTAAYEHCKSGEALLKRLEKWEDVSSAELQVYEVKVRSFWVHLRDFSQRVEDTKDKIDKTVQLYEFFDKAYEWALEGMRHLACVSMEDCGMSEKCQSVITCLETYRSQHPPIPDAHFQEMKDLAGELKSEQGLKQWKFAWSKCQETKQMFEKKLEMALRSRRDSDSKSVRSDSSRRNSDVSTKHQERSSSISFSCRKAFGGAWGRDRLSSHSSTSSTPSSPSGIRLDARDTVRTPTGSPYSIEHRIPHSTPVRSHRLTRSISTDESPQSPHAEGLASTSPSGTSVQTNRRVLRKTQSFDTAGSEPVSQYGTCQRTLSEPARRGNTGVFIKGLEVSSTEVIDRPYSPRLPPIHGWSSIDAHRSGSPAPETRSKGSKLRHIVDEMVTTEREYVRSLRYIIDNYFPEMERAVLPQDLRGKRSVIFGNLEKLVDFHSQYFLKELESCCNHPLRVSHCFLRHQDQFSLYALYSKNKPKSDTLLASHGNNFFKHKQMELGDKMDLASYLLKPIQRMSKYALLLKDLIKEVSEVQEQELTYLRAAAEMVKFQLRHGNDLLAMDAIRDCDVNLKEQGQLVRQDEFTISYGRKKCQRHVFLFEDLVLFSKPKRIEGGLDVYIYKHSFKTADVGMTETSGENSLRFEIWFRRRTSKNQTYILQSSTADIKHAWTCDIARILWQQATRNKEIRMQEMVSMGVGNKPFLDIKPSDAAINDRAIDYIMKGRGARTRASIAVSLFDHSNPFKRAAVNTPVSGTPVSGGPSSSTLLGPLNLHMYSSQSLLAGERPLIGPCIEEDELEHETSSQPSMTTESSGSSSHCLSGSGSSGSDSGCVSSHLPEALSEEPSSPCDSSCYSSITSPTQEKPCFNSQYISAGDAQVIGPSTIV

Target ORF information:

RefSeq Version	XM_009303344.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303344.2

ORF Insert Sequence:

ATGGACTCTG AATCTCTGGA CAGCTGTATC CAGAGCACAC TGTCCGCTCT CTACCCTCCA 
TTTGAAGCCA CTGCGGCCAC TGTACTGTGC CAGGTGTTAG ATGTGGTGGA GACAACATAC 
CGTGGAGACG GACTGCGATA CATCATCGAC TTTCTGGTGC CCGCTAAACA CATCCTACAG 
AGCATTCAGC AGGATGCATG CTTCCCATAC TGTGGTTTTC TGTTCCGTCA TGAAGGATGG 
CCACTGTGTG TCCATGAGAA GGTCATAATC CAGCTCTCCT CTTTGGACTG GCGTGTATTA 
CGGCCCAGCG ACTTCTACTT GCAGGTGACA CCATCTGCCA AAAGCCCCCC GCGCATCAAA 
GTGAAGTGTC TGGCTGTTAC CGGGCAGCAT GCGGAGGAGC TGGACGTGCC GGAGCTAGCA 
TATACCTCTC TATTCACCAT GGACTGGCTG GACTCCATCA ACCAGGAGAG GAGTGTGGTC 
AGAAGAGCTG CCCTGGAACA CTGTCTTTTA TCAGCGGATA ATGATATTTT TAGGGTACCG 
TGGGAGGATA TTGTTCACCC GGAATTTATC AGCAGACCCT CTAAAGTTGC AGAACAAAGT 
GAAAGTAGAA GGGTTGCCAA AGAAGTTGCA AATGGAGACG GGCGAGACTC TGATGTAGAA 
CAAGACAACA ATCCTGAGGA CATACAATGT AGAATTTTTT ATGACACTTC CACGAACCAT 
TCAGGGGATA TGGATTGTCA GAGCAAGGAA GTTAAGCTCC TCCCTGTTCT TAGTGAGATT 
AATAAAGATA TCAGTGGGAG GAGCTCTAGC AGCACTGCGG ACGATTCAGA GGGCGAATAT 
GTGGAGCTAG CTGATATCTC CTTACCACGC TTTTCCCCAC AAAAGGGCTC TCTTACTCAA 
GCCATTAGCA TGAACTATAG AAATCTACAT AAACCTCAGA CTCCTGTTTC CACTCAGCTT 
AAAACCAAAC CAGATGAGAG TCTTTTGAAG AATGGTGCAT GCTCTCAAAG CATGGTGTGT 
GCCATGATGA TCGAGGAAAG CCTGAGTGAT ACCTACCAAA CAGTGATCCT TACTCCCACT 
GCTCCTGCAG AGGCAGAGCG TCCGCTCCAG CAGGTGGACA TGTCTGAACC CGCCTGTGCA 
GCAGGTACAG CTTCCTTTGA CAGTGTGTCT GAGCATGAGT CTGAGAGAGA GCTATTATCT 
CAACACTCTG ACTGTGAGGA TGCTTCACTA TCTGATATAT CAACAATTAA CATATCGCAA 
ACAGACATTA AACCAATCAA CACATCACAA CTAGACAACA ATGTAGTCTG CAAATCACAA 
GACAGCGAAC CATACAGTAC ATCACAATTA GACAAGAATC TAGTGAACAC ATCACAAACA 
GATACTGAAT CATTGAGCAT GTCTCAATTA GACGATAATT TGGACTGCAC ATCACATCCA 
GATGTTAACC AGAAACCATC AAGTATGTCT CAACTAGAAC TTGAACCACT AACCGTATCT 
CAGCTTGACA GCAAGCTAAT CAGCACATCA CAACCTGATA GCACATCACT AGATGTAGAA 
CCAGTTAGCA CATCTAATGT AGACAACAAA CCAGTCAGCA CATCACAACC GGACAGCACA 
TTACAGTTGG TCAATGAACC AGTCAAGACA TCACAAGATG GTGAACCAGT AAGCAGATTT 
CATGTAGACC ACAAACCAGT AAGCACATTA CAACCAGACA GCCAACCAGT CATCACATCA 
GTCAATGAGC CATTTAGGAC ATCACAACCA GACAATAAAT CAGTAAGCAC AACTCAACTA 
GACAGCAAAC CAGTTAGTGT ATCACAACAC AACAAACAAG AAAGCAAATC TGTCAACACA 
TCTGTTGACA ATGAGCCAGT CAAGACATCA CAGCCAGACA CTGAATCACT AAGAATATCT 
CAATTTGACA GCAAACCAGT CAGCAACTCA CAACTAGGCA GCAAACAAGT CAGCATTAAA 
CAACTAGTCA ATGAGATATT TAGTACACCA CAACCAGACA ATAAATCAGT GAGCACAACT 
CAACTAGAAA GAAAACTAGT TTGCGGATCA CAAGACAACA AACAAGAAAG CAAATCTGTC 
AACACATTAG TAGACAGTGA GCCAGTCAAG ACATCACAGA CAGACACAGA ACTGCTAAGA 
ATATCACAGT TTGATAGCAA ACCAGTCAGC AACTCACAAC TAGATGGCAA ACAAGTCAGC 
ATTTCACAGC TAGTCAATGA GATATTTAGT ACACCACAAC CAGACAATAA ATCAGTAAGC 
CCAACTCAAC TAGACAGTAA GCTAGTTAGC ACATTACAAG ACAAACAAGA AAGCAAATCT 
GTCAATAATT CAATAGACAA TGAGCCAGTC AAGACATCAC AGGCAGACAC AGAACCGCTA 
AAAAGGTCTC AGTTTGACAG CAACCCAGTC AGCAACTCAC AGCTAGATGG CAAACAAGTC 
AGCATTTCAC AACTAGTCAA TGAGATATTT AGTACACCAC AACCAGACAA TAAATCAGTG 
AGCACAACTC AACTAGATGG CAACATAGAT AGAACATCAC AATTAGACAG CAAACAAGAA 
AGTGTCTCTC AAACAGACAG CAAACCAGTC AGCATATCAC CAGACAATGA GCCAGTGAAG 
ACATCACAAC CACAACTAGA AAGCACATTA CACCCAAACA GCAAACAAGC AAGCATGTCA 
CAACTAGTCA ATGAACACAT CCAGACCTCA CAGCCAGACC TAAAACCAGT AAGCATTGCT 
CAACTCTACA GTGAAGATGC TAAACCAGAT GGATACGCTG ATGTCAAACC AATAACCACT 
GAGCTAGACA ATGGACTAGT GAGCACATCA CAACCAGATA ACCAACCAGT TGACACACCA 
CAAGCAGAAT GTACAACAGT TAACATAACC TTACCAGAGG GCAACCCCAA TGACACATCA 
TATATTGTCA ACCTGCCGCT AAGTACATCA CAACCAAACA GCAAAACAGT TCATGTGGAG 
CAAGCAGACA TAAATGTAAT TAATACATCA CAGCCAGAGA CTGAAATGTT ATCTTGTGTT 
TTGGAAAGGG AGAACTCAGA ACAGGTTTCT CCAGCTTATA TTGAACTCAT TGTGTCAGAT 
CCTTATGCAG AAGTCAGATG TGAATCTAAA GAGCTGAGAG TTAATTGTGA TCTTGAAGGC 
AGTTGTGGTT CACAGAACAA CAGAGCGTCA CTTGCCGAGA CAGTAGCTGA GAGTGTGTCT 
TCGCAAAATC AGATTGATCA AACAGGCAAT AGCCTGTATC AGATACACAA ACCCATCCAT 
ACACCATCTG AGCAAATGCA TGATCTTCCT ACCATAAAAG AAGACCATCA GACATTCAGT 
AGCAGTTCTC ATAACACGAA TGAATCTGTT GGTGATTTAA GTCCCATTGC AGAAGAAGAT 
GGTGGGTCTG TTACAGCTGC TCTGTATAGT GAGCATGTAG CCTTAGTTTC TGTCACCACA 
GTGCCTGGGG AGAGTCCAGT TACACTCATT TCACAGGGTC AGGTGGGGAC AGAGTCTCCT 
GAGGACAGAA TCAACCAGAC AGAGGTGGAA CTTCAGACAA AGGAAGAACG TGCAGTACAA 
AACGTAGCTG GAATGCCACC TTGTAAGGGG GCTGAAATGG TAGAAAAGAA AAGCAGTAGC 
GAGGCAGAAA TCAAAACTGA TGAGAATGAT GTAGTGGCAG TGTGTAGGCA AGAGGAAGTT 
GAGACTTCAC AAACAGGTCT AGTAGATGCT ACGCCCAAGT CTGACGTGCA AGTTCAGGAG 
GAGGTCAGCT CCCCTGTAGA AACAGATGAG AAGAAACAAA CCAGTGTTCA AGAAAAAGGG 
GTGGAGGAAG AGGAGGTCAA GGCCAAAACT GTGAGGGAGC AAGAGGCCAT AGTGACCAGC 
AATTCTGAAA ATAGAGTCCA GTCAGCATGC TCTGAAGTGC TTGATCCAGC CTCTGATGTC 
CCGAACACAA ATGGACACAT GCATGCTCAG GACACTCCTA CCACTGAACT AAAAGGGCAA 
ATAACAAAGG AGGAGGATGG AAAAGGGGCA GAGGAGAAAA GGCAAGAGGA TGAGATTGCT 
TCTTCTGTCA ATGGCTCTCA AAAAGAGCAT CAGCAGCAAG CAGACATGGA CTCCAGCTTC 
CATCAGCAGG AAGAGCAAGC ATGCAGTCAG AAAGTAGACA GCACTGTTTG CCACCTGTCT 
GCTAAAACTG AGAGTGAAGA CCCCTCAGCA GTTCCTTCAC CACAGCCACC AGTCGATCAT 
AGAGCACAAC CAGTCAAGAG CAAGGCCCAT GACATAAATC CTGATGTCCT AAGCTCAGGT 
GTGCTCTGCC TGACTGGAAC AAGAGATAAA TCAGGCCATG CCTTGGTCAC TGTGACAATG 
AGAAACACTG TCTGGCTGAA TCCAAACTGC AACAGTGGAG AGCTAGTGCG AATCATGGTG 
TATTTCTGCA GTACACTCAG GAAGGAAGTA AGTGCTTTGG GATTGACTGT CCTGGTGGAT 
GCCCGCAGGT GTTCACCTGT TCCCGCCCTT TTCAAAGCCT TCAACATCCT CCAGGAAGCC 
ATGCCGGGAT GCATTCATAC TGTACTGCTG CTGGCCGACA GAGATCTGGC TCTACGCATG 
GAGAAAACCT CTTCTATACA GGTGGAGCTG CTGACATCAC TCAAGTCTCT CTATAAACAT 
GTGGACGTTT CTCAGCTTCC CACTGAGTTT GAAGGCACTT TCCCTTATGC ACACAGCAGC 
TGGGTCAGCT TTAGAATGAG GTTGGAGCAG TTGACCAGTC ATTGTGAAGA TGCTGTGAGC 
CTGCTTCAGA ACACCATAAG CAAACTGGAG TCCACAGTGC TGCCCCCTAC AGCAGAGGAG 
GCTCAACTCT TACTGTGTAA GTACAGAGAG CTGATGAGGA CGGTTCTGGA GGACAGCAGG 
CTGGTGCGAC TACAGTTGGA GGGAGGAGCA GCTCTGTCCC GTTTACGAAA AGAAGAGACC 
AGCGTTAGTC TCACAGAGGA CTACAGAGAT GCCATTGAAA GTGCTGGCCG TTTGTATAAC 
CAAGTGGACG AGCTGGTTCA CAGACTGGTG ATGCTGTCCA ATAAATGCAC ACAGGAACTG 
GAATTCATCA TGGAGTTTAA GAGCCTGGAG GAGGGATTCA AAGAGGTTAG TCAGTGGATA 
GAAGAGGTGG GAGAGTCTCG TCTGCAGACG CTGAGCGAGC TGGAGGATTC CTTGGAGCAG 
TTACATCATA AGCAGAGCAT CTTCAGAGAA TTTTACACAG CTGCATATGA ACACTGTAAG 
AGTGGTGAAG CTCTCCTCAA GCGTTTAGAG AAGTGGGAAG ACGTCTCCTC TGCAGAGCTG 
CAAGTGTATG AGGTGAAAGT ACGTTCATTC TGGGTGCATC TGCGGGATTT CTCTCAGCGA 
GTGGAGGACA CCAAGGATAA AATAGACAAG ACTGTTCAAC TCTATGAGTT CTTTGATAAG 
GCATATGAAT GGGCTCTAGA GGGCATGCGT CATTTGGCGT GTGTCAGTAT GGAGGATTGT 
GGCATGTCTG AAAAGTGTCA GAGCGTGATC ACGTGTTTGG AGACTTACCG CAGTCAGCAC 
CCCCCGATCC CTGATGCACA CTTCCAGGAG ATGAAGGACC TCGCTGGGGA ACTGAAAAGC 
GAACAGGGCC TCAAGCAGTG GAAGTTTGCC TGGTCCAAGT GTCAGGAGAC CAAGCAGATG 
TTTGAAAAGA AGCTGGAGAT GGCCCTGCGT TCCCGCCGTG ACAGCGACTC CAAGTCAGTA 
AGGAGTGACT CTTCTCGTCG TAACTCAGAT GTTAGCACTA AACACCAAGA GCGTAGCAGT 
AGCATCTCCT TCTCATGTCG GAAAGCGTTC GGCGGAGCCT GGGGTCGAGA TAGACTTTCC 
TCACATTCTA GCACATCCTC AACTCCCAGT AGCCCTTCAG GCATTCGTCT GGATGCCCGT 
GACACTGTCC GAACTCCAAC AGGAAGTCCA TACAGCATTG AGCACAGAAT CCCCCACAGC 
ACCCCTGTCA GATCCCACAG GCTAACACGT AGCATCTCCA CAGATGAGTC TCCTCAGAGC 
CCACATGCAG AGGGGCTAGC GTCCACAAGC CCAAGCGGCA CTTCTGTTCA AACAAACCGC 
AGGGTTTTGC GGAAGACCCA GAGCTTCGAT ACAGCTGGCA GTGAGCCTGT GTCACAGTAC 
GGGACCTGTC AGAGGACTTT GAGTGAGCCT GCACGCAGAG GAAACACAGG GGTGTTCATT 
AAGGGTCTGG AGGTGAGCAG CACTGAAGTG ATTGATAGGC CGTACAGTCC ACGACTACCA 
CCCATCCATG GATGGTCTTC AATTGATGCA CACCGCAGCG GGAGTCCTGC TCCAGAAACG 
CGCAGCAAGG GCAGCAAGTT ACGACACATT GTAGATGAGA TGGTGACAAC AGAGCGGGAA 
TATGTGCGTT CCCTGCGGTA TATCATTGAC AACTATTTCC CTGAGATGGA GCGCGCTGTC 
CTGCCGCAGG ATCTGCGGGG AAAGCGGAGC GTCATATTTG GAAATCTGGA GAAACTGGTG 
GACTTTCATA GTCAGTATTT CCTAAAAGAG CTGGAGAGCT GCTGTAATCA TCCTCTACGC 
GTCAGCCACT GTTTCCTACG ACATCAAGAC CAGTTTAGCC TCTATGCATT ATACAGCAAG 
AACAAGCCAA AATCAGACAC ACTCCTGGCC AGCCATGGAA ACAACTTCTT CAAGCATAAG 
CAGATGGAGC TGGGAGATAA GATGGACCTG GCATCGTACC TGCTGAAGCC CATCCAGCGC 
ATGAGTAAAT ACGCTCTTCT GCTGAAAGAC CTGATCAAAG AAGTGAGCGA GGTCCAGGAG 
CAGGAACTTA CATACCTGCG AGCCGCAGCA GAGATGGTCA AGTTTCAGCT CCGCCATGGC 
AATGATCTAC TCGCCATGGA CGCTATCAGA GATTGTGATG TGAACCTAAA AGAGCAAGGA 
CAGTTGGTTC GTCAAGATGA GTTCACGATT TCATATGGCA GAAAGAAGTG CCAGAGACAC 
GTGTTCCTAT TTGAGGACCT AGTGCTCTTC AGCAAACCCA AGCGCATCGA GGGTGGACTA 
GATGTTTACA TATACAAGCA TTCTTTTAAG ACGGCTGATG TGGGTATGAC AGAGACGTCA 
GGTGAGAACA GTCTGAGGTT TGAAATTTGG TTTCGGAGGA GAACTTCAAA GAACCAGACC 
TACATTCTCC AGTCCAGCAC TGCTGACATC AAACATGCCT GGACTTGCGA CATCGCACGA 
ATACTGTGGC AGCAGGCCAC GCGAAACAAA GAAATTCGAA TGCAGGAGAT GGTCTCAATG 
GGTGTGGGCA ATAAACCCTT CCTGGACATT AAACCCAGTG ATGCTGCAAT CAACGACAGA 
GCCATCGACT ACATTATGAA GGGCAGAGGA GCTAGAACGA GAGCCTCCAT TGCTGTTTCT 
CTGTTTGACC ACTCAAACCC ATTCAAAAGA GCTGCAGTAA ACACCCCCGT CAGCGGGACC 
CCGGTGTCTG GCGGTCCTTC CTCATCCACC CTGCTGGGGC CCTTGAATCT GCACATGTAC 
AGCAGCCAAA GTCTGTTGGC GGGGGAGAGA CCTCTGATTG GCCCCTGCAT CGAGGAGGAC 
GAGCTGGAGC ATGAGACCAG TAGTCAGCCC TCTATGACTA CAGAGAGTTC GGGTTCGTCG 
TCTCACTGTC TGTCTGGCAG TGGCTCTAGC GGATCAGACA GCGGCTGTGT GTCCAGTCAC 
CTTCCTGAGG CTTTGTCTGA GGAGCCCAGC TCACCTTGTG ACTCCTCGTG TTATTCCTCC 
ATCACCTCTC CCACGCAGGA GAAGCCCTGC TTTAACAGCC AGTATATCTC AGCGGGGGAT 
GCTCAGGTGA TCGGACCCTC TACTATAGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg4 ( XM_009303345.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301620.1 Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X2, mRNA.

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CloneID	ODa39867
Clone ID Related Accession (Same CDS sequence)	XM_009303345.3 , XM_009303345.2
Accession Version	XM_009303345.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein plekhg4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303345.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTG AATCTCTGGA CAGCTGTATC CAGAGCACAC TGTCCGCTCT CTACCCTCCA 
TTTGAAGCCA CTGCGGCCAC TGTACTGTGC CAGGTGTTAG ATGTGGTGGA GACAACATAC 
CGTGGAGACG GACTGCGATA CATCATCGAC TTTCTGGTGC CCGCTAAACA CATCCTACAG 
AGCATTCAGC AGGATGCATG CTTCCCATAC TGTGGTTTTC TGTTCCGTCA TGAAGGATGG 
CCACTGTGTG TCCATGAGAA GGTCATAATC CAGCTCTCCT CTTTGGACTG GCGTGTATTA 
CGGCCCAGCG ACTTCTACTT GCAGGTGACA CCATCTGCCA AAAGCCCCCC GCGCATCAAA 
GTGAAGTGTC TGGCTGTTAC CGGGCAGCAT GCGGAGGAGC TGGACGTGCC GGAGCTAGCA 
TATACCTCTC TATTCACCAT GGACTGGCTG GACTCCATCA ACCAGGAGAG GAGTGTGGTC 
AGAAGAGCTG CCCTGGAACA CTGTCTTTTA TCAGCGGATA ATGATATTTT TAGGGTACCG 
TGGGAGGATA TTGTTCACCC GGAATTTATC AGCAGACCCT CTAAAGTTGC AGAACAAAGT 
GAAAGTAGAA GGGTTGCCAA AGAAGTTGCA AATGGAGACG GGCGAGACTC TGATGTAGAA 
CAAGACAACA ATCCTGAGGA CATACAATGT AGAATTTTTT ATGACACTTC CACGAACCAT 
TCAGGGGATA TGGATTGTCA GAGCAAGGAA GTTAAGCTCC TCCCTGTTCT TAGTGAGATT 
AATAAAGATA TCAGTGGGAG GAGCTCTAGC AGCACTGCGG ACGATTCAGA GGGCGAATAT 
GTGGAGCTAG CTGATATCTC CTTACCACGC TTTTCCCCAC AAAAGGGCTC TCTTACTCAA 
GCCATTAGCA TGAACTATAG AAATCTACAT AAACCTCAGA CTCCTGTTTC CACTCAGCTT 
AAAACCAAAC CAGATGAGAG TCTTTTGAAG AATGGTGCAT GCTCTCAAAG CATGGTGTGT 
GCCATGATGA TCGAGGAAAG CCTGAGTGAT ACCTACCAAA CAGTGATCCT TACTCCCACT 
GCTCCTGCAG AGGCAGAGCG TCCGCTCCAG CAGGTGGACA TGTCTGAACC CGCCTGTGCA 
GCAGGTACAG CTTCCTTTGA CAGTGTGTCT GAGCATGAGT CTGAGAGAGA GCTATTATCT 
CAACACTCTG ACTGTGAGGA TGCTTCACTA TCTGATATAT CAACAATTAA CATATCGCAA 
ACAGACATTA AACCAATCAA CACATCACAA CTAGACAACA ATGTAGTCTG CAAATCACAA 
GACAGCGAAC CATACAGTAC ATCACAATTA GACAAGAATC TAGTGAACAC ATCACAAACA 
GATACTGAAT CATTGAGCAT GTCTCAATTA GACGATAATT TGGACTGCAC ATCACATCCA 
GATGTTAACC AGAAACCATC AAGTATGTCT CAACTAGAAC TTGAACCACT AACCGTATCT 
CAGCTTGACA GCAAGCTAAT CAGCACATCA CAACCTGATA GCACATCACT AGATGTAGAA 
CCAGTTAGCA CATCTAATGT AGACAACAAA CCAGTCAGCA CATCACAACC GGACAGCACA 
TTACAGTTGG TCAATGAACC AGTCAAGACA TCACAAGATG GTGAACCAGT AAGCAGATTT 
CATGTAGACC ACAAACCAGT AAGCACATTA CAACCAGACA GCCAACCAGT CATCACATCA 
GTCAATGAGC CATTTAGGAC ATCACAACCA GACAATAAAT CAGTAAGCAC AACTCAACTA 
GACAGCAAAC CAGTTAGTGT ATCACAACAC AACAAACAAG AAAGCAAATC TGTCAACACA 
TCTGTTGACA ATGAGCCAGT CAAGACATCA CAGCCAGACA CTGAATCACT AAGAATATCT 
CAATTTGACA GCAAACCAGT CAGCAACTCA CAACTAGGCA GCAAACAAGT CAGCATTAAA 
CAACTAGTCA ATGAGATATT TAGTACACCA CAACCAGACA ATAAATCAGT GAGCACAACT 
CAACTAGAAA GAAAACTAGT TTGCGGATCA CAAGACAACA AACAAGAAAG CAAATCTGTC 
AACACATTAG TAGACAGTGA GCCAGTCAAG ACATCACAGA CAGACACAGA ACTGCTAAGA 
ATATCACAGT TTGATAGCAA ACCAGTCAGC AACTCACAAC TAGATGGCAA ACAAGTCAGC 
ATTTCACAGC TAGTCAATGA GATATTTAGT ACACCACAAC CAGACAATAA ATCAGTAAGC 
CCAACTCAAC TAGACAGTAA GCTAGTTAGC ACATTACAAG ACAAACAAGA AAGCAAATCT 
GTCAATAATT CAATAGACAA TGAGCCAGTC AAGACATCAC AGGCAGACAC AGAACCGCTA 
AAAAGGTCTC AGTTTGACAG CAACCCAGTC AGCAACTCAC AGCTAGATGG CAAACAAGTC 
AGCATTTCAC AACTAGTCAA TGAGATATTT AGTACACCAC AACCAGACAA TAAATCAGTG 
AGCACAACTC AACTAGATGG CAACATAGAT AGAACATCAC AATTAGACAG CAAACAAGAA 
AGTGTCTCTC AAACAGACAG CAAACCAGTC AGCATATCAC CAGACAATGA GCCAGTGAAG 
ACATCACAAC CACAACTAGA AAGCACATTA CACCCAAACA GCAAACAAGC AAGCATGTCA 
CAACTAGTCA ATGAACACAT CCAGACCTCA CAGCCAGACC TAAAACCAGT AAGCATTGCT 
CAACTCTACA GTGAAGATGC TAAACCAGAT GGATACGCTG ATGTCAAACC AATAACCACT 
GAGCTAGACA ATGGACTAGT GAGCACATCA CAACCAGATA ACCAACCAGT TGACACACCA 
CAAGCAGAAT GTACAACAGT TAACATAACC TTACCAGAGG GCAACCCCAA TGACACATCA 
TATATTGTCA ACCTGCCGCT AAGTACATCA CAACCAAACA GCAAAACAGT TCATGTGGAG 
CAAGCAGACA TAAATGTAAT TAATACATCA CAGCCAGAGA CTGAAATGTT ATCTTGTGTT 
TTGGAAAGGG AGAACTCAGA ACAGGTTTCT CCAGCTTATA TTGAACTCAT TGTGTCAGAT 
CCTTATGCAG AAGTCAGATG TGAATCTAAA GAGCTGAGAG TTAATTGTGA TCTTGAAGGC 
AGTTGTGGTT CACAGAACAA CAGAGCGTCA CTTGCCGAGA CAGTAGCTGA GAGTGTGTCT 
TCGCAAAATC AGATTGATCA AACAGGCAAT AGCCTGTATC AGATACACAA ACCCATCCAT 
ACACCATCTG AGCAAATGCA TGATCTTCCT ACCATAAAAG AAGACCATCA GACATTCAGT 
AGCAGTTCTC ATAACACGAA TGAATCTGTT GGTGATTTAA GTCCCATTGC AGAAGAAGAT 
GGTGGGTCTG TTACAGCTGC TCTGTATAGT GAGCATGTAG CCTTAGTTTC TGTCACCACA 
GTGCCTGGGG AGAGTCCAGT TACACTCATT TCACAGGGTC AGGTGGGGAC AGAGTCTCCT 
GAGGACAGAA TCAACCAGAC AGAGGTGGAA CTTCAGACAA AGGAAGAACG TGCAGTACAA 
AACGTAGCTG GAATGCCACC TTGTAAGGGG GCTGAAATGG TAGAAAAGAA AAGCAGTAGC 
GAGGCAGAAA TCAAAACTGA TGAGAATGAT GTAGTGGCAG TGTGTAGGCA AGAGGAAGTT 
GAGACTTCAC AAACAGGTCT AGTAGATGCT ACGCCCAAGT CTGACGTGCA AGTTCAGGAG 
GAGGTCAGCT CCCCTGTAGA AACAGATGAG AAGAAACAAA CCAGTGTTCA AGAAAAAGGG 
GTGGAGGAAG AGGAGGTCAA GGCCAAAACT GTGAGGGAGC AAGAGGCCAT AGTGACCAGC 
AATTCTGAAA ATAGAGTCCA GTCAGCATGC TCTGAAGTGC TTGATCCAGC CTCTGATGTC 
CCGAACACAA ATGGACACAT GCATGCTCAG GACACTCCTA CCACTGAACT AAAAGGGCAA 
ATAACAAAGG AGGAGGATGG AAAAGGGGCA GAGGAGAAAA GGCAAGAGGA TGAGATTGCT 
TCTTCTGTCA ATGGCTCTCA AAAAGAGCAT CAGCAGCAAG CAGACATGGA CTCCAGCTTC 
CATCAGCAGG AAGAGCAAGC ATGCAGTCAG AAAGTAGACA GCACTGTTTG CCACCTGTCT 
GCTAAAACTG AGAGTGAAGA CCCCTCAGCA GTTCCTTCAC CACAGCCACC AGTCGATCAT 
AGAGCACAAC CAGTCAAGAG CAAGGCCCAT GACATAAATC CTGATGTCCT AAGCTCAGGT 
GTGCTCTGCC TGACTGGAAC AAGAGATAAA TCAGGCCATG CCTTGGTCAC TGTGACAATG 
AGAAACACTG TCTGGCTGAA TCCAAACTGC AACAGTGGAG AGCTAGTGCG AATCATGGTG 
TATTTCTGCA GTACACTCAG GAAGGAAGTA AGTGCTTTGG GATTGACTGT CCTGGTGGAT 
GCCCGCAGGT GTTCACCTGT TCCCGCCCTT TTCAAAGCCT TCAACATCCT CCAGGAAGCC 
ATGCCGGGAT GCATTCATAC TGTACTGCTG CTGGCCGACA GAGATCTGGC TCTACGCATG 
GAGAAAACCT CTTCTATACA GGTGGAGCTG CTGACATCAC TCAAGTCTCT CTATAAACAT 
GTGGACGTTT CTCAGCTTCC CACTGAGTTT GAAGGCACTT TCCCTTATGC ACACAGCAGC 
TGGGTCAGCT TTAGAATGAG GTTGGAGCAG TTGACCAGTC ATTGTGAAGA TGCTGTGAGC 
CTGCTTCAGA ACACCATAAG CAAACTGGAG TCCACAGTGC TGCCCCCTAC AGCAGAGGAG 
GCTCAACTCT TACTGTGTAA GTACAGAGAG CTGATGAGGA CGGTTCTGGA GGACAGCAGG 
CTGGTGCGAC TACAGTTGGA GGGAGGAGCA GCTCTGTCCC GTTTACGAAA AGAAGAGACC 
AGCGTTAGTC TCACAGAGGA CTACAGAGAT GCCATTGAAA GTGCTGGCCG TTTGTATAAC 
CAAGTGGACG AGCTGGTTCA CAGACTGGTG ATGCTGTCCA ATAAATGCAC ACAGGAACTG 
GAATTCATCA TGGAGTTTAA GAGCCTGGAG GAGGGATTCA AAGAGGTTAG TCAGTGGATA 
GAAGAGGTGG GAGAGTCTCG TCTGCAGACG CTGAGCGAGC TGGAGGATTC CTTGGAGCAG 
TTACATCATA AGCAGAGCAT CTTCAGAGAA TTTTACACAG CTGCATATGA ACACTGTAAG 
AGTGGTGAAG CTCTCCTCAA GCGTTTAGAG AAGTGGGAAG ACGTCTCCTC TGCAGAGCTG 
CAAGTGTATG AGGTGAAAGT ACGTTCATTC TGGGTGCATC TGCGGGATTT CTCTCAGCGA 
GTGGAGGACA CCAAGGATAA AATAGACAAG ACTGTTCAAC TCTATGAGTT CTTTGATAAG 
GCATATGAAT GGGCTCTAGA GGGCATGCGT CATTTGGCGT GTGTCAGTAT GGAGGATTGT 
GGCATGTCTG AAAAGTGTCA GAGCGTGATC ACGTGTTTGG AGACTTACCG CAGTCAGCAC 
CCCCCGATCC CTGATGCACA CTTCCAGGAG ATGAAGGACC TCGCTGGGGA ACTGAAAAGC 
GAACAGGGCC TCAAGCAGTG GAAGTTTGCC TGGTCCAAGT GTCAGGAGAC CAAGCAGATG 
TTTGAAAAGA AGCTGGAGAT GGCCCTGCGT TCCCGCCGTG ACAGCGACTC CAAGTCAGTA 
AGGAGTGACT CTTCTCGTCG TAACTCAGAT GTTAGCACTA AACACCAAGA GCGTAGCAGT 
AGCATCTCCT TCTCATGTCG GAAAGCGTTC GGCGGAGCCT GGGGTCGAGA TAGACTTTCC 
TCACATTCTA GCACATCCTC AACTCCCAGT AGCCCTTCAG GCATTCGTCT GGATGCCCGT 
GACACTGTCC GAACTCCAAC AGGAAGTCCA TACAGCATTG AGCACAGAAT CCCCCACAGC 
ACCCCTGTCA GATCCCACAG GCTAACACGT AGCATCTCCA CAGATGAGTC TCCTCAGAGC 
CCACATGCAG AGGGGCTAGC GTCCACAAGC CCAAGCGGCA CTTCTGTTCA AACAAACCGC 
AGGGTTTTGC GGAAGACCCA GAGCTTCGAT ACAGCTGGCA GTGAGCCTGT GTCACAGTAC 
GGGACCTGTC AGAGGACTTT GAGTGAGCCT GCACGCAGAG GAAACACAGG GGTGTTCATT 
AAGGGTCTGG AGGTGAGCAG CACTGAAGTG ATTGATAGGC CGTACAGTCC ACGACTACCA 
CCCATCCATG GATGGTCTTC AATTGATGCA CACCGCAGCG GGAGTCCTGC TCCAGAAACG 
CGCAGCAAGG GCAGCAAGTT ACGACACATT GTAGATGAGA TGGTGACAAC AGAGCGGGAA 
TATGTGCGTT CCCTGCGGTA TATCATTGAC AACTATTTCC CTGAGATGGA GCGCGCTGTC 
CTGCCGCAGG ATCTGCGGGG AAAGCGGAGC GTCATATTTG GAAATCTGGA GAAACTGGTG 
GACTTTCATA GTCAGTATTT CCTAAAAGAG CTGGAGAGCT GCTGTAATCA TCCTCTACGC 
GTCAGCCACT GTTTCCTACG ACATCAAGAC CAGTTTAGCC TCTATGCATT ATACAGCAAG 
AACAAGCCAA AATCAGACAC ACTCCTGGCC AGCCATGGAA ACAACTTCTT CAAGCATAAG 
CAGATGGAGC TGGGAGATAA GATGGACCTG GCATCGTACC TGCTGAAGCC CATCCAGCGC 
ATGAGTAAAT ACGCTCTTCT GCTGAAAGAC CTGATCAAAG AAGTGAGCGA GGTCCAGGAG 
CAGGAACTTA CATACCTGCG AGCCGCAGCA GAGATGGTCA AGTTTCAGCT CCGCCATGGC 
AATGATCTAC TCGCCATGGA CGCTATCAGA GATTGTGATG TGAACCTAAA AGAGCAAGGA 
CAGTTGGTTC GTCAAGATGA GTTCACGATT TCATATGGCA GAAAGAAGTG CCAGAGACAC 
GTGTTCCTAT TTGAGGACCT AGTGCTCTTC AGCAAACCCA AGCGCATCGA GGGTGGACTA 
GATGTTTACA TATACAAGCA TTCTTTTAAG ACGGCTGATG TGGGTATGAC AGAGACGTCA 
GGTGAGAACA GTCTGAGGTT TGAAATTTGG TTTCGGAGGA GAACTTCAAA GAACCAGACC 
TACATTCTCC AGTCCAGCAC TGCTGACATC AAACATGCCT GGACTTGCGA CATCGCACGA 
ATACTGTGGC AGCAGGCCAC GCGAAACAAA GAAATTCGAA TGCAGGAGAT GGTCTCAATG 
GGTGTGGGCA ATAAACCCTT CCTGGACATT AAACCCAGTG ATGCTGCAAT CAACGACAGA 
GCCATCGACT ACATTATGAA GGGCAGAGGA GCTAGAACGA GAGCCTCCAT TGCTGTTTCT 
CTGTTTGACC ACTCAAACCC ATTCAAAAGA GCTGCAGTAA ACACCCCCGT CAGCGGGACC 
CCGGTGTCTG GCGGTCCTTC CTCATCCACC CTGCTGGGGC CCTTGAATCT GCACATGTAC 
AGCAGCCAAA GTCTGTTGGC GGGGGAGAGA CCTCTGATTG GCCCCTGCAT CGAGGAGGAC 
GAGCTGGAGC ATGAGACCAG TAGTCAGCCC TCTATGACTA CAGAGAGTTC GGGTTCGTCG 
TCTCACTGTC TGTCTGGCAG TGGCTCTAGC GGATCAGACA GCGGCTGTGT GTCCAGTCAC 
CTTCCTGAGG CTTTGTCTGA GGAGCCCAGC TCACCTTGTG ACTCCTCGTG TTATTCCTCC 
ATCACCTCTC CCACGCAGGA GAAGCCCTGC TTTAACAGCC AGTATATCTC AGCGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301620.1
CDS	175..8034
Translation	MDSESLDSCIQSTLSALYPPFEATAATVLCQVLDVVETTYRGDGLRYIIDFLVPAKHILQSIQQDACFPYCGFLFRHEGWPLCVHEKVIIQLSSLDWRVLRPSDFYLQVTPSAKSPPRIKVKCLAVTGQHAEELDVPELAYTSLFTMDWLDSINQERSVVRRAALEHCLLSADNDIFRVPWEDIVHPEFISRPSKVAEQSESRRVAKEVANGDGRDSDVEQDNNPEDIQCRIFYDTSTNHSGDMDCQSKEVKLLPVLSEINKDISGRSSSSTADDSEGEYVELADISLPRFSPQKGSLTQAISMNYRNLHKPQTPVSTQLKTKPDESLLKNGACSQSMVCAMMIEESLSDTYQTVILTPTAPAEAERPLQQVDMSEPACAAGTASFDSVSEHESERELLSQHSDCEDASLSDISTINISQTDIKPINTSQLDNNVVCKSQDSEPYSTSQLDKNLVNTSQTDTESLSMSQLDDNLDCTSHPDVNQKPSSMSQLELEPLTVSQLDSKLISTSQPDSTSLDVEPVSTSNVDNKPVSTSQPDSTLQLVNEPVKTSQDGEPVSRFHVDHKPVSTLQPDSQPVITSVNEPFRTSQPDNKSVSTTQLDSKPVSVSQHNKQESKSVNTSVDNEPVKTSQPDTESLRISQFDSKPVSNSQLGSKQVSIKQLVNEIFSTPQPDNKSVSTTQLERKLVCGSQDNKQESKSVNTLVDSEPVKTSQTDTELLRISQFDSKPVSNSQLDGKQVSISQLVNEIFSTPQPDNKSVSPTQLDSKLVSTLQDKQESKSVNNSIDNEPVKTSQADTEPLKRSQFDSNPVSNSQLDGKQVSISQLVNEIFSTPQPDNKSVSTTQLDGNIDRTSQLDSKQESVSQTDSKPVSISPDNEPVKTSQPQLESTLHPNSKQASMSQLVNEHIQTSQPDLKPVSIAQLYSEDAKPDGYADVKPITTELDNGLVSTSQPDNQPVDTPQAECTTVNITLPEGNPNDTSYIVNLPLSTSQPNSKTVHVEQADINVINTSQPETEMLSCVLERENSEQVSPAYIELIVSDPYAEVRCESKELRVNCDLEGSCGSQNNRASLAETVAESVSSQNQIDQTGNSLYQIHKPIHTPSEQMHDLPTIKEDHQTFSSSSHNTNESVGDLSPIAEEDGGSVTAALYSEHVALVSVTTVPGESPVTLISQGQVGTESPEDRINQTEVELQTKEERAVQNVAGMPPCKGAEMVEKKSSSEAEIKTDENDVVAVCRQEEVETSQTGLVDATPKSDVQVQEEVSSPVETDEKKQTSVQEKGVEEEEVKAKTVREQEAIVTSNSENRVQSACSEVLDPASDVPNTNGHMHAQDTPTTELKGQITKEEDGKGAEEKRQEDEIASSVNGSQKEHQQQADMDSSFHQQEEQACSQKVDSTVCHLSAKTESEDPSAVPSPQPPVDHRAQPVKSKAHDINPDVLSSGVLCLTGTRDKSGHALVTVTMRNTVWLNPNCNSGELVRIMVYFCSTLRKEVSALGLTVLVDARRCSPVPALFKAFNILQEAMPGCIHTVLLLADRDLALRMEKTSSIQVELLTSLKSLYKHVDVSQLPTEFEGTFPYAHSSWVSFRMRLEQLTSHCEDAVSLLQNTISKLESTVLPPTAEEAQLLLCKYRELMRTVLEDSRLVRLQLEGGAALSRLRKEETSVSLTEDYRDAIESAGRLYNQVDELVHRLVMLSNKCTQELEFIMEFKSLEEGFKEVSQWIEEVGESRLQTLSELEDSLEQLHHKQSIFREFYTAAYEHCKSGEALLKRLEKWEDVSSAELQVYEVKVRSFWVHLRDFSQRVEDTKDKIDKTVQLYEFFDKAYEWALEGMRHLACVSMEDCGMSEKCQSVITCLETYRSQHPPIPDAHFQEMKDLAGELKSEQGLKQWKFAWSKCQETKQMFEKKLEMALRSRRDSDSKSVRSDSSRRNSDVSTKHQERSSSISFSCRKAFGGAWGRDRLSSHSSTSSTPSSPSGIRLDARDTVRTPTGSPYSIEHRIPHSTPVRSHRLTRSISTDESPQSPHAEGLASTSPSGTSVQTNRRVLRKTQSFDTAGSEPVSQYGTCQRTLSEPARRGNTGVFIKGLEVSSTEVIDRPYSPRLPPIHGWSSIDAHRSGSPAPETRSKGSKLRHIVDEMVTTEREYVRSLRYIIDNYFPEMERAVLPQDLRGKRSVIFGNLEKLVDFHSQYFLKELESCCNHPLRVSHCFLRHQDQFSLYALYSKNKPKSDTLLASHGNNFFKHKQMELGDKMDLASYLLKPIQRMSKYALLLKDLIKEVSEVQEQELTYLRAAAEMVKFQLRHGNDLLAMDAIRDCDVNLKEQGQLVRQDEFTISYGRKKCQRHVFLFEDLVLFSKPKRIEGGLDVYIYKHSFKTADVGMTETSGENSLRFEIWFRRRTSKNQTYILQSSTADIKHAWTCDIARILWQQATRNKEIRMQEMVSMGVGNKPFLDIKPSDAAINDRAIDYIMKGRGARTRASIAVSLFDHSNPFKRAAVNTPVSGTPVSGGPSSSTLLGPLNLHMYSSQSLLAGERPLIGPCIEEDELEHETSSQPSMTTESSGSSSHCLSGSGSSGSDSGCVSSHLPEALSEEPSSPCDSSCYSSITSPTQEKPCFNSQYISAV

Target ORF information:

RefSeq Version	XM_009303345.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303345.3

ORF Insert Sequence:

ATGGACTCTG AATCTCTGGA CAGCTGTATC CAGAGCACAC TGTCCGCTCT CTACCCTCCA 
TTTGAAGCCA CTGCGGCCAC TGTACTGTGC CAGGTGTTAG ATGTGGTGGA GACAACATAC 
CGTGGAGACG GACTGCGATA CATCATCGAC TTTCTGGTGC CCGCTAAACA CATCCTACAG 
AGCATTCAGC AGGATGCATG CTTCCCATAC TGTGGTTTTC TGTTCCGTCA TGAAGGATGG 
CCACTGTGTG TCCATGAGAA GGTCATAATC CAGCTCTCCT CTTTGGACTG GCGTGTATTA 
CGGCCCAGCG ACTTCTACTT GCAGGTGACA CCATCTGCCA AAAGCCCCCC GCGCATCAAA 
GTGAAGTGTC TGGCTGTTAC CGGGCAGCAT GCGGAGGAGC TGGACGTGCC GGAGCTAGCA 
TATACCTCTC TATTCACCAT GGACTGGCTG GACTCCATCA ACCAGGAGAG GAGTGTGGTC 
AGAAGAGCTG CCCTGGAACA CTGTCTTTTA TCAGCGGATA ATGATATTTT TAGGGTACCG 
TGGGAGGATA TTGTTCACCC GGAATTTATC AGCAGACCCT CTAAAGTTGC AGAACAAAGT 
GAAAGTAGAA GGGTTGCCAA AGAAGTTGCA AATGGAGACG GGCGAGACTC TGATGTAGAA 
CAAGACAACA ATCCTGAGGA CATACAATGT AGAATTTTTT ATGACACTTC CACGAACCAT 
TCAGGGGATA TGGATTGTCA GAGCAAGGAA GTTAAGCTCC TCCCTGTTCT TAGTGAGATT 
AATAAAGATA TCAGTGGGAG GAGCTCTAGC AGCACTGCGG ACGATTCAGA GGGCGAATAT 
GTGGAGCTAG CTGATATCTC CTTACCACGC TTTTCCCCAC AAAAGGGCTC TCTTACTCAA 
GCCATTAGCA TGAACTATAG AAATCTACAT AAACCTCAGA CTCCTGTTTC CACTCAGCTT 
AAAACCAAAC CAGATGAGAG TCTTTTGAAG AATGGTGCAT GCTCTCAAAG CATGGTGTGT 
GCCATGATGA TCGAGGAAAG CCTGAGTGAT ACCTACCAAA CAGTGATCCT TACTCCCACT 
GCTCCTGCAG AGGCAGAGCG TCCGCTCCAG CAGGTGGACA TGTCTGAACC CGCCTGTGCA 
GCAGGTACAG CTTCCTTTGA CAGTGTGTCT GAGCATGAGT CTGAGAGAGA GCTATTATCT 
CAACACTCTG ACTGTGAGGA TGCTTCACTA TCTGATATAT CAACAATTAA CATATCGCAA 
ACAGACATTA AACCAATCAA CACATCACAA CTAGACAACA ATGTAGTCTG CAAATCACAA 
GACAGCGAAC CATACAGTAC ATCACAATTA GACAAGAATC TAGTGAACAC ATCACAAACA 
GATACTGAAT CATTGAGCAT GTCTCAATTA GACGATAATT TGGACTGCAC ATCACATCCA 
GATGTTAACC AGAAACCATC AAGTATGTCT CAACTAGAAC TTGAACCACT AACCGTATCT 
CAGCTTGACA GCAAGCTAAT CAGCACATCA CAACCTGATA GCACATCACT AGATGTAGAA 
CCAGTTAGCA CATCTAATGT AGACAACAAA CCAGTCAGCA CATCACAACC GGACAGCACA 
TTACAGTTGG TCAATGAACC AGTCAAGACA TCACAAGATG GTGAACCAGT AAGCAGATTT 
CATGTAGACC ACAAACCAGT AAGCACATTA CAACCAGACA GCCAACCAGT CATCACATCA 
GTCAATGAGC CATTTAGGAC ATCACAACCA GACAATAAAT CAGTAAGCAC AACTCAACTA 
GACAGCAAAC CAGTTAGTGT ATCACAACAC AACAAACAAG AAAGCAAATC TGTCAACACA 
TCTGTTGACA ATGAGCCAGT CAAGACATCA CAGCCAGACA CTGAATCACT AAGAATATCT 
CAATTTGACA GCAAACCAGT CAGCAACTCA CAACTAGGCA GCAAACAAGT CAGCATTAAA 
CAACTAGTCA ATGAGATATT TAGTACACCA CAACCAGACA ATAAATCAGT GAGCACAACT 
CAACTAGAAA GAAAACTAGT TTGCGGATCA CAAGACAACA AACAAGAAAG CAAATCTGTC 
AACACATTAG TAGACAGTGA GCCAGTCAAG ACATCACAGA CAGACACAGA ACTGCTAAGA 
ATATCACAGT TTGATAGCAA ACCAGTCAGC AACTCACAAC TAGATGGCAA ACAAGTCAGC 
ATTTCACAGC TAGTCAATGA GATATTTAGT ACACCACAAC CAGACAATAA ATCAGTAAGC 
CCAACTCAAC TAGACAGTAA GCTAGTTAGC ACATTACAAG ACAAACAAGA AAGCAAATCT 
GTCAATAATT CAATAGACAA TGAGCCAGTC AAGACATCAC AGGCAGACAC AGAACCGCTA 
AAAAGGTCTC AGTTTGACAG CAACCCAGTC AGCAACTCAC AGCTAGATGG CAAACAAGTC 
AGCATTTCAC AACTAGTCAA TGAGATATTT AGTACACCAC AACCAGACAA TAAATCAGTG 
AGCACAACTC AACTAGATGG CAACATAGAT AGAACATCAC AATTAGACAG CAAACAAGAA 
AGTGTCTCTC AAACAGACAG CAAACCAGTC AGCATATCAC CAGACAATGA GCCAGTGAAG 
ACATCACAAC CACAACTAGA AAGCACATTA CACCCAAACA GCAAACAAGC AAGCATGTCA 
CAACTAGTCA ATGAACACAT CCAGACCTCA CAGCCAGACC TAAAACCAGT AAGCATTGCT 
CAACTCTACA GTGAAGATGC TAAACCAGAT GGATACGCTG ATGTCAAACC AATAACCACT 
GAGCTAGACA ATGGACTAGT GAGCACATCA CAACCAGATA ACCAACCAGT TGACACACCA 
CAAGCAGAAT GTACAACAGT TAACATAACC TTACCAGAGG GCAACCCCAA TGACACATCA 
TATATTGTCA ACCTGCCGCT AAGTACATCA CAACCAAACA GCAAAACAGT TCATGTGGAG 
CAAGCAGACA TAAATGTAAT TAATACATCA CAGCCAGAGA CTGAAATGTT ATCTTGTGTT 
TTGGAAAGGG AGAACTCAGA ACAGGTTTCT CCAGCTTATA TTGAACTCAT TGTGTCAGAT 
CCTTATGCAG AAGTCAGATG TGAATCTAAA GAGCTGAGAG TTAATTGTGA TCTTGAAGGC 
AGTTGTGGTT CACAGAACAA CAGAGCGTCA CTTGCCGAGA CAGTAGCTGA GAGTGTGTCT 
TCGCAAAATC AGATTGATCA AACAGGCAAT AGCCTGTATC AGATACACAA ACCCATCCAT 
ACACCATCTG AGCAAATGCA TGATCTTCCT ACCATAAAAG AAGACCATCA GACATTCAGT 
AGCAGTTCTC ATAACACGAA TGAATCTGTT GGTGATTTAA GTCCCATTGC AGAAGAAGAT 
GGTGGGTCTG TTACAGCTGC TCTGTATAGT GAGCATGTAG CCTTAGTTTC TGTCACCACA 
GTGCCTGGGG AGAGTCCAGT TACACTCATT TCACAGGGTC AGGTGGGGAC AGAGTCTCCT 
GAGGACAGAA TCAACCAGAC AGAGGTGGAA CTTCAGACAA AGGAAGAACG TGCAGTACAA 
AACGTAGCTG GAATGCCACC TTGTAAGGGG GCTGAAATGG TAGAAAAGAA AAGCAGTAGC 
GAGGCAGAAA TCAAAACTGA TGAGAATGAT GTAGTGGCAG TGTGTAGGCA AGAGGAAGTT 
GAGACTTCAC AAACAGGTCT AGTAGATGCT ACGCCCAAGT CTGACGTGCA AGTTCAGGAG 
GAGGTCAGCT CCCCTGTAGA AACAGATGAG AAGAAACAAA CCAGTGTTCA AGAAAAAGGG 
GTGGAGGAAG AGGAGGTCAA GGCCAAAACT GTGAGGGAGC AAGAGGCCAT AGTGACCAGC 
AATTCTGAAA ATAGAGTCCA GTCAGCATGC TCTGAAGTGC TTGATCCAGC CTCTGATGTC 
CCGAACACAA ATGGACACAT GCATGCTCAG GACACTCCTA CCACTGAACT AAAAGGGCAA 
ATAACAAAGG AGGAGGATGG AAAAGGGGCA GAGGAGAAAA GGCAAGAGGA TGAGATTGCT 
TCTTCTGTCA ATGGCTCTCA AAAAGAGCAT CAGCAGCAAG CAGACATGGA CTCCAGCTTC 
CATCAGCAGG AAGAGCAAGC ATGCAGTCAG AAAGTAGACA GCACTGTTTG CCACCTGTCT 
GCTAAAACTG AGAGTGAAGA CCCCTCAGCA GTTCCTTCAC CACAGCCACC AGTCGATCAT 
AGAGCACAAC CAGTCAAGAG CAAGGCCCAT GACATAAATC CTGATGTCCT AAGCTCAGGT 
GTGCTCTGCC TGACTGGAAC AAGAGATAAA TCAGGCCATG CCTTGGTCAC TGTGACAATG 
AGAAACACTG TCTGGCTGAA TCCAAACTGC AACAGTGGAG AGCTAGTGCG AATCATGGTG 
TATTTCTGCA GTACACTCAG GAAGGAAGTA AGTGCTTTGG GATTGACTGT CCTGGTGGAT 
GCCCGCAGGT GTTCACCTGT TCCCGCCCTT TTCAAAGCCT TCAACATCCT CCAGGAAGCC 
ATGCCGGGAT GCATTCATAC TGTACTGCTG CTGGCCGACA GAGATCTGGC TCTACGCATG 
GAGAAAACCT CTTCTATACA GGTGGAGCTG CTGACATCAC TCAAGTCTCT CTATAAACAT 
GTGGACGTTT CTCAGCTTCC CACTGAGTTT GAAGGCACTT TCCCTTATGC ACACAGCAGC 
TGGGTCAGCT TTAGAATGAG GTTGGAGCAG TTGACCAGTC ATTGTGAAGA TGCTGTGAGC 
CTGCTTCAGA ACACCATAAG CAAACTGGAG TCCACAGTGC TGCCCCCTAC AGCAGAGGAG 
GCTCAACTCT TACTGTGTAA GTACAGAGAG CTGATGAGGA CGGTTCTGGA GGACAGCAGG 
CTGGTGCGAC TACAGTTGGA GGGAGGAGCA GCTCTGTCCC GTTTACGAAA AGAAGAGACC 
AGCGTTAGTC TCACAGAGGA CTACAGAGAT GCCATTGAAA GTGCTGGCCG TTTGTATAAC 
CAAGTGGACG AGCTGGTTCA CAGACTGGTG ATGCTGTCCA ATAAATGCAC ACAGGAACTG 
GAATTCATCA TGGAGTTTAA GAGCCTGGAG GAGGGATTCA AAGAGGTTAG TCAGTGGATA 
GAAGAGGTGG GAGAGTCTCG TCTGCAGACG CTGAGCGAGC TGGAGGATTC CTTGGAGCAG 
TTACATCATA AGCAGAGCAT CTTCAGAGAA TTTTACACAG CTGCATATGA ACACTGTAAG 
AGTGGTGAAG CTCTCCTCAA GCGTTTAGAG AAGTGGGAAG ACGTCTCCTC TGCAGAGCTG 
CAAGTGTATG AGGTGAAAGT ACGTTCATTC TGGGTGCATC TGCGGGATTT CTCTCAGCGA 
GTGGAGGACA CCAAGGATAA AATAGACAAG ACTGTTCAAC TCTATGAGTT CTTTGATAAG 
GCATATGAAT GGGCTCTAGA GGGCATGCGT CATTTGGCGT GTGTCAGTAT GGAGGATTGT 
GGCATGTCTG AAAAGTGTCA GAGCGTGATC ACGTGTTTGG AGACTTACCG CAGTCAGCAC 
CCCCCGATCC CTGATGCACA CTTCCAGGAG ATGAAGGACC TCGCTGGGGA ACTGAAAAGC 
GAACAGGGCC TCAAGCAGTG GAAGTTTGCC TGGTCCAAGT GTCAGGAGAC CAAGCAGATG 
TTTGAAAAGA AGCTGGAGAT GGCCCTGCGT TCCCGCCGTG ACAGCGACTC CAAGTCAGTA 
AGGAGTGACT CTTCTCGTCG TAACTCAGAT GTTAGCACTA AACACCAAGA GCGTAGCAGT 
AGCATCTCCT TCTCATGTCG GAAAGCGTTC GGCGGAGCCT GGGGTCGAGA TAGACTTTCC 
TCACATTCTA GCACATCCTC AACTCCCAGT AGCCCTTCAG GCATTCGTCT GGATGCCCGT 
GACACTGTCC GAACTCCAAC AGGAAGTCCA TACAGCATTG AGCACAGAAT CCCCCACAGC 
ACCCCTGTCA GATCCCACAG GCTAACACGT AGCATCTCCA CAGATGAGTC TCCTCAGAGC 
CCACATGCAG AGGGGCTAGC GTCCACAAGC CCAAGCGGCA CTTCTGTTCA AACAAACCGC 
AGGGTTTTGC GGAAGACCCA GAGCTTCGAT ACAGCTGGCA GTGAGCCTGT GTCACAGTAC 
GGGACCTGTC AGAGGACTTT GAGTGAGCCT GCACGCAGAG GAAACACAGG GGTGTTCATT 
AAGGGTCTGG AGGTGAGCAG CACTGAAGTG ATTGATAGGC CGTACAGTCC ACGACTACCA 
CCCATCCATG GATGGTCTTC AATTGATGCA CACCGCAGCG GGAGTCCTGC TCCAGAAACG 
CGCAGCAAGG GCAGCAAGTT ACGACACATT GTAGATGAGA TGGTGACAAC AGAGCGGGAA 
TATGTGCGTT CCCTGCGGTA TATCATTGAC AACTATTTCC CTGAGATGGA GCGCGCTGTC 
CTGCCGCAGG ATCTGCGGGG AAAGCGGAGC GTCATATTTG GAAATCTGGA GAAACTGGTG 
GACTTTCATA GTCAGTATTT CCTAAAAGAG CTGGAGAGCT GCTGTAATCA TCCTCTACGC 
GTCAGCCACT GTTTCCTACG ACATCAAGAC CAGTTTAGCC TCTATGCATT ATACAGCAAG 
AACAAGCCAA AATCAGACAC ACTCCTGGCC AGCCATGGAA ACAACTTCTT CAAGCATAAG 
CAGATGGAGC TGGGAGATAA GATGGACCTG GCATCGTACC TGCTGAAGCC CATCCAGCGC 
ATGAGTAAAT ACGCTCTTCT GCTGAAAGAC CTGATCAAAG AAGTGAGCGA GGTCCAGGAG 
CAGGAACTTA CATACCTGCG AGCCGCAGCA GAGATGGTCA AGTTTCAGCT CCGCCATGGC 
AATGATCTAC TCGCCATGGA CGCTATCAGA GATTGTGATG TGAACCTAAA AGAGCAAGGA 
CAGTTGGTTC GTCAAGATGA GTTCACGATT TCATATGGCA GAAAGAAGTG CCAGAGACAC 
GTGTTCCTAT TTGAGGACCT AGTGCTCTTC AGCAAACCCA AGCGCATCGA GGGTGGACTA 
GATGTTTACA TATACAAGCA TTCTTTTAAG ACGGCTGATG TGGGTATGAC AGAGACGTCA 
GGTGAGAACA GTCTGAGGTT TGAAATTTGG TTTCGGAGGA GAACTTCAAA GAACCAGACC 
TACATTCTCC AGTCCAGCAC TGCTGACATC AAACATGCCT GGACTTGCGA CATCGCACGA 
ATACTGTGGC AGCAGGCCAC GCGAAACAAA GAAATTCGAA TGCAGGAGAT GGTCTCAATG 
GGTGTGGGCA ATAAACCCTT CCTGGACATT AAACCCAGTG ATGCTGCAAT CAACGACAGA 
GCCATCGACT ACATTATGAA GGGCAGAGGA GCTAGAACGA GAGCCTCCAT TGCTGTTTCT 
CTGTTTGACC ACTCAAACCC ATTCAAAAGA GCTGCAGTAA ACACCCCCGT CAGCGGGACC 
CCGGTGTCTG GCGGTCCTTC CTCATCCACC CTGCTGGGGC CCTTGAATCT GCACATGTAC 
AGCAGCCAAA GTCTGTTGGC GGGGGAGAGA CCTCTGATTG GCCCCTGCAT CGAGGAGGAC 
GAGCTGGAGC ATGAGACCAG TAGTCAGCCC TCTATGACTA CAGAGAGTTC GGGTTCGTCG 
TCTCACTGTC TGTCTGGCAG TGGCTCTAGC GGATCAGACA GCGGCTGTGT GTCCAGTCAC 
CTTCCTGAGG CTTTGTCTGA GGAGCCCAGC TCACCTTGTG ACTCCTCGTG TTATTCCTCC 
ATCACCTCTC CCACGCAGGA GAAGCCCTGC TTTAACAGCC AGTATATCTC AGCGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg4 ( XM_009303345.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301620.1 Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X2, mRNA.

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CloneID	ODa39867
Clone ID Related Accession (Same CDS sequence)	XM_009303345.3 , XM_009303345.2
Accession Version	XM_009303345.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein plekhg4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTG AATCTCTGGA CAGCTGTATC CAGAGCACAC TGTCCGCTCT CTACCCTCCA 
TTTGAAGCCA CTGCGGCCAC TGTACTGTGC CAGGTGTTAG ATGTGGTGGA GACAACATAC 
CGTGGAGACG GACTGCGATA CATCATCGAC TTTCTGGTGC CCGCTAAACA CATCCTACAG 
AGCATTCAGC AGGATGCATG CTTCCCATAC TGTGGTTTTC TGTTCCGTCA TGAAGGATGG 
CCACTGTGTG TCCATGAGAA GGTCATAATC CAGCTCTCCT CTTTGGACTG GCGTGTATTA 
CGGCCCAGCG ACTTCTACTT GCAGGTGACA CCATCTGCCA AAAGCCCCCC GCGCATCAAA 
GTGAAGTGTC TGGCTGTTAC CGGGCAGCAT GCGGAGGAGC TGGACGTGCC GGAGCTAGCA 
TATACCTCTC TATTCACCAT GGACTGGCTG GACTCCATCA ACCAGGAGAG GAGTGTGGTC 
AGAAGAGCTG CCCTGGAACA CTGTCTTTTA TCAGCGGATA ATGATATTTT TAGGGTACCG 
TGGGAGGATA TTGTTCACCC GGAATTTATC AGCAGACCCT CTAAAGTTGC AGAACAAAGT 
GAAAGTAGAA GGGTTGCCAA AGAAGTTGCA AATGGAGACG GGCGAGACTC TGATGTAGAA 
CAAGACAACA ATCCTGAGGA CATACAATGT AGAATTTTTT ATGACACTTC CACGAACCAT 
TCAGGGGATA TGGATTGTCA GAGCAAGGAA GTTAAGCTCC TCCCTGTTCT TAGTGAGATT 
AATAAAGATA TCAGTGGGAG GAGCTCTAGC AGCACTGCGG ACGATTCAGA GGGCGAATAT 
GTGGAGCTAG CTGATATCTC CTTACCACGC TTTTCCCCAC AAAAGGGCTC TCTTACTCAA 
GCCATTAGCA TGAACTATAG AAATCTACAT AAACCTCAGA CTCCTGTTTC CACTCAGCTT 
AAAACCAAAC CAGATGAGAG TCTTTTGAAG AATGGTGCAT GCTCTCAAAG CATGGTGTGT 
GCCATGATGA TCGAGGAAAG CCTGAGTGAT ACCTACCAAA CAGTGATCCT TACTCCCACT 
GCTCCTGCAG AGGCAGAGCG TCCGCTCCAG CAGGTGGACA TGTCTGAACC CGCCTGTGCA 
GCAGGTACAG CTTCCTTTGA CAGTGTGTCT GAGCATGAGT CTGAGAGAGA GCTATTATCT 
CAACACTCTG ACTGTGAGGA TGCTTCACTA TCTGATATAT CAACAATTAA CATATCGCAA 
ACAGACATTA AACCAATCAA CACATCACAA CTAGACAACA ATGTAGTCTG CAAATCACAA 
GACAGCGAAC CATACAGTAC ATCACAATTA GACAAGAATC TAGTGAACAC ATCACAAACA 
GATACTGAAT CATTGAGCAT GTCTCAATTA GACGATAATT TGGACTGCAC ATCACATCCA 
GATGTTAACC AGAAACCATC AAGTATGTCT CAACTAGAAC TTGAACCACT AACCGTATCT 
CAGCTTGACA GCAAGCTAAT CAGCACATCA CAACCTGATA GCACATCACT AGATGTAGAA 
CCAGTTAGCA CATCTAATGT AGACAACAAA CCAGTCAGCA CATCACAACC GGACAGCACA 
TTACAGTTGG TCAATGAACC AGTCAAGACA TCACAAGATG GTGAACCAGT AAGCAGATTT 
CATGTAGACC ACAAACCAGT AAGCACATTA CAACCAGACA GCCAACCAGT CATCACATCA 
GTCAATGAGC CATTTAGGAC ATCACAACCA GACAATAAAT CAGTAAGCAC AACTCAACTA 
GACAGCAAAC CAGTTAGTGT ATCACAACAC AACAAACAAG AAAGCAAATC TGTCAACACA 
TCTGTTGACA ATGAGCCAGT CAAGACATCA CAGCCAGACA CTGAATCACT AAGAATATCT 
CAATTTGACA GCAAACCAGT CAGCAACTCA CAACTAGGCA GCAAACAAGT CAGCATTAAA 
CAACTAGTCA ATGAGATATT TAGTACACCA CAACCAGACA ATAAATCAGT GAGCACAACT 
CAACTAGAAA GAAAACTAGT TTGCGGATCA CAAGACAACA AACAAGAAAG CAAATCTGTC 
AACACATTAG TAGACAGTGA GCCAGTCAAG ACATCACAGA CAGACACAGA ACTGCTAAGA 
ATATCACAGT TTGATAGCAA ACCAGTCAGC AACTCACAAC TAGATGGCAA ACAAGTCAGC 
ATTTCACAGC TAGTCAATGA GATATTTAGT ACACCACAAC CAGACAATAA ATCAGTAAGC 
CCAACTCAAC TAGACAGTAA GCTAGTTAGC ACATTACAAG ACAAACAAGA AAGCAAATCT 
GTCAATAATT CAATAGACAA TGAGCCAGTC AAGACATCAC AGGCAGACAC AGAACCGCTA 
AAAAGGTCTC AGTTTGACAG CAACCCAGTC AGCAACTCAC AGCTAGATGG CAAACAAGTC 
AGCATTTCAC AACTAGTCAA TGAGATATTT AGTACACCAC AACCAGACAA TAAATCAGTG 
AGCACAACTC AACTAGATGG CAACATAGAT AGAACATCAC AATTAGACAG CAAACAAGAA 
AGTGTCTCTC AAACAGACAG CAAACCAGTC AGCATATCAC CAGACAATGA GCCAGTGAAG 
ACATCACAAC CACAACTAGA AAGCACATTA CACCCAAACA GCAAACAAGC AAGCATGTCA 
CAACTAGTCA ATGAACACAT CCAGACCTCA CAGCCAGACC TAAAACCAGT AAGCATTGCT 
CAACTCTACA GTGAAGATGC TAAACCAGAT GGATACGCTG ATGTCAAACC AATAACCACT 
GAGCTAGACA ATGGACTAGT GAGCACATCA CAACCAGATA ACCAACCAGT TGACACACCA 
CAAGCAGAAT GTACAACAGT TAACATAACC TTACCAGAGG GCAACCCCAA TGACACATCA 
TATATTGTCA ACCTGCCGCT AAGTACATCA CAACCAAACA GCAAAACAGT TCATGTGGAG 
CAAGCAGACA TAAATGTAAT TAATACATCA CAGCCAGAGA CTGAAATGTT ATCTTGTGTT 
TTGGAAAGGG AGAACTCAGA ACAGGTTTCT CCAGCTTATA TTGAACTCAT TGTGTCAGAT 
CCTTATGCAG AAGTCAGATG TGAATCTAAA GAGCTGAGAG TTAATTGTGA TCTTGAAGGC 
AGTTGTGGTT CACAGAACAA CAGAGCGTCA CTTGCCGAGA CAGTAGCTGA GAGTGTGTCT 
TCGCAAAATC AGATTGATCA AACAGGCAAT AGCCTGTATC AGATACACAA ACCCATCCAT 
ACACCATCTG AGCAAATGCA TGATCTTCCT ACCATAAAAG AAGACCATCA GACATTCAGT 
AGCAGTTCTC ATAACACGAA TGAATCTGTT GGTGATTTAA GTCCCATTGC AGAAGAAGAT 
GGTGGGTCTG TTACAGCTGC TCTGTATAGT GAGCATGTAG CCTTAGTTTC TGTCACCACA 
GTGCCTGGGG AGAGTCCAGT TACACTCATT TCACAGGGTC AGGTGGGGAC AGAGTCTCCT 
GAGGACAGAA TCAACCAGAC AGAGGTGGAA CTTCAGACAA AGGAAGAACG TGCAGTACAA 
AACGTAGCTG GAATGCCACC TTGTAAGGGG GCTGAAATGG TAGAAAAGAA AAGCAGTAGC 
GAGGCAGAAA TCAAAACTGA TGAGAATGAT GTAGTGGCAG TGTGTAGGCA AGAGGAAGTT 
GAGACTTCAC AAACAGGTCT AGTAGATGCT ACGCCCAAGT CTGACGTGCA AGTTCAGGAG 
GAGGTCAGCT CCCCTGTAGA AACAGATGAG AAGAAACAAA CCAGTGTTCA AGAAAAAGGG 
GTGGAGGAAG AGGAGGTCAA GGCCAAAACT GTGAGGGAGC AAGAGGCCAT AGTGACCAGC 
AATTCTGAAA ATAGAGTCCA GTCAGCATGC TCTGAAGTGC TTGATCCAGC CTCTGATGTC 
CCGAACACAA ATGGACACAT GCATGCTCAG GACACTCCTA CCACTGAACT AAAAGGGCAA 
ATAACAAAGG AGGAGGATGG AAAAGGGGCA GAGGAGAAAA GGCAAGAGGA TGAGATTGCT 
TCTTCTGTCA ATGGCTCTCA AAAAGAGCAT CAGCAGCAAG CAGACATGGA CTCCAGCTTC 
CATCAGCAGG AAGAGCAAGC ATGCAGTCAG AAAGTAGACA GCACTGTTTG CCACCTGTCT 
GCTAAAACTG AGAGTGAAGA CCCCTCAGCA GTTCCTTCAC CACAGCCACC AGTCGATCAT 
AGAGCACAAC CAGTCAAGAG CAAGGCCCAT GACATAAATC CTGATGTCCT AAGCTCAGGT 
GTGCTCTGCC TGACTGGAAC AAGAGATAAA TCAGGCCATG CCTTGGTCAC TGTGACAATG 
AGAAACACTG TCTGGCTGAA TCCAAACTGC AACAGTGGAG AGCTAGTGCG AATCATGGTG 
TATTTCTGCA GTACACTCAG GAAGGAAGTA AGTGCTTTGG GATTGACTGT CCTGGTGGAT 
GCCCGCAGGT GTTCACCTGT TCCCGCCCTT TTCAAAGCCT TCAACATCCT CCAGGAAGCC 
ATGCCGGGAT GCATTCATAC TGTACTGCTG CTGGCCGACA GAGATCTGGC TCTACGCATG 
GAGAAAACCT CTTCTATACA GGTGGAGCTG CTGACATCAC TCAAGTCTCT CTATAAACAT 
GTGGACGTTT CTCAGCTTCC CACTGAGTTT GAAGGCACTT TCCCTTATGC ACACAGCAGC 
TGGGTCAGCT TTAGAATGAG GTTGGAGCAG TTGACCAGTC ATTGTGAAGA TGCTGTGAGC 
CTGCTTCAGA ACACCATAAG CAAACTGGAG TCCACAGTGC TGCCCCCTAC AGCAGAGGAG 
GCTCAACTCT TACTGTGTAA GTACAGAGAG CTGATGAGGA CGGTTCTGGA GGACAGCAGG 
CTGGTGCGAC TACAGTTGGA GGGAGGAGCA GCTCTGTCCC GTTTACGAAA AGAAGAGACC 
AGCGTTAGTC TCACAGAGGA CTACAGAGAT GCCATTGAAA GTGCTGGCCG TTTGTATAAC 
CAAGTGGACG AGCTGGTTCA CAGACTGGTG ATGCTGTCCA ATAAATGCAC ACAGGAACTG 
GAATTCATCA TGGAGTTTAA GAGCCTGGAG GAGGGATTCA AAGAGGTTAG TCAGTGGATA 
GAAGAGGTGG GAGAGTCTCG TCTGCAGACG CTGAGCGAGC TGGAGGATTC CTTGGAGCAG 
TTACATCATA AGCAGAGCAT CTTCAGAGAA TTTTACACAG CTGCATATGA ACACTGTAAG 
AGTGGTGAAG CTCTCCTCAA GCGTTTAGAG AAGTGGGAAG ACGTCTCCTC TGCAGAGCTG 
CAAGTGTATG AGGTGAAAGT ACGTTCATTC TGGGTGCATC TGCGGGATTT CTCTCAGCGA 
GTGGAGGACA CCAAGGATAA AATAGACAAG ACTGTTCAAC TCTATGAGTT CTTTGATAAG 
GCATATGAAT GGGCTCTAGA GGGCATGCGT CATTTGGCGT GTGTCAGTAT GGAGGATTGT 
GGCATGTCTG AAAAGTGTCA GAGCGTGATC ACGTGTTTGG AGACTTACCG CAGTCAGCAC 
CCCCCGATCC CTGATGCACA CTTCCAGGAG ATGAAGGACC TCGCTGGGGA ACTGAAAAGC 
GAACAGGGCC TCAAGCAGTG GAAGTTTGCC TGGTCCAAGT GTCAGGAGAC CAAGCAGATG 
TTTGAAAAGA AGCTGGAGAT GGCCCTGCGT TCCCGCCGTG ACAGCGACTC CAAGTCAGTA 
AGGAGTGACT CTTCTCGTCG TAACTCAGAT GTTAGCACTA AACACCAAGA GCGTAGCAGT 
AGCATCTCCT TCTCATGTCG GAAAGCGTTC GGCGGAGCCT GGGGTCGAGA TAGACTTTCC 
TCACATTCTA GCACATCCTC AACTCCCAGT AGCCCTTCAG GCATTCGTCT GGATGCCCGT 
GACACTGTCC GAACTCCAAC AGGAAGTCCA TACAGCATTG AGCACAGAAT CCCCCACAGC 
ACCCCTGTCA GATCCCACAG GCTAACACGT AGCATCTCCA CAGATGAGTC TCCTCAGAGC 
CCACATGCAG AGGGGCTAGC GTCCACAAGC CCAAGCGGCA CTTCTGTTCA AACAAACCGC 
AGGGTTTTGC GGAAGACCCA GAGCTTCGAT ACAGCTGGCA GTGAGCCTGT GTCACAGTAC 
GGGACCTGTC AGAGGACTTT GAGTGAGCCT GCACGCAGAG GAAACACAGG GGTGTTCATT 
AAGGGTCTGG AGGTGAGCAG CACTGAAGTG ATTGATAGGC CGTACAGTCC ACGACTACCA 
CCCATCCATG GATGGTCTTC AATTGATGCA CACCGCAGCG GGAGTCCTGC TCCAGAAACG 
CGCAGCAAGG GCAGCAAGTT ACGACACATT GTAGATGAGA TGGTGACAAC AGAGCGGGAA 
TATGTGCGTT CCCTGCGGTA TATCATTGAC AACTATTTCC CTGAGATGGA GCGCGCTGTC 
CTGCCGCAGG ATCTGCGGGG AAAGCGGAGC GTCATATTTG GAAATCTGGA GAAACTGGTG 
GACTTTCATA GTCAGTATTT CCTAAAAGAG CTGGAGAGCT GCTGTAATCA TCCTCTACGC 
GTCAGCCACT GTTTCCTACG ACATCAAGAC CAGTTTAGCC TCTATGCATT ATACAGCAAG 
AACAAGCCAA AATCAGACAC ACTCCTGGCC AGCCATGGAA ACAACTTCTT CAAGCATAAG 
CAGATGGAGC TGGGAGATAA GATGGACCTG GCATCGTACC TGCTGAAGCC CATCCAGCGC 
ATGAGTAAAT ACGCTCTTCT GCTGAAAGAC CTGATCAAAG AAGTGAGCGA GGTCCAGGAG 
CAGGAACTTA CATACCTGCG AGCCGCAGCA GAGATGGTCA AGTTTCAGCT CCGCCATGGC 
AATGATCTAC TCGCCATGGA CGCTATCAGA GATTGTGATG TGAACCTAAA AGAGCAAGGA 
CAGTTGGTTC GTCAAGATGA GTTCACGATT TCATATGGCA GAAAGAAGTG CCAGAGACAC 
GTGTTCCTAT TTGAGGACCT AGTGCTCTTC AGCAAACCCA AGCGCATCGA GGGTGGACTA 
GATGTTTACA TATACAAGCA TTCTTTTAAG ACGGCTGATG TGGGTATGAC AGAGACGTCA 
GGTGAGAACA GTCTGAGGTT TGAAATTTGG TTTCGGAGGA GAACTTCAAA GAACCAGACC 
TACATTCTCC AGTCCAGCAC TGCTGACATC AAACATGCCT GGACTTGCGA CATCGCACGA 
ATACTGTGGC AGCAGGCCAC GCGAAACAAA GAAATTCGAA TGCAGGAGAT GGTCTCAATG 
GGTGTGGGCA ATAAACCCTT CCTGGACATT AAACCCAGTG ATGCTGCAAT CAACGACAGA 
GCCATCGACT ACATTATGAA GGGCAGAGGA GCTAGAACGA GAGCCTCCAT TGCTGTTTCT 
CTGTTTGACC ACTCAAACCC ATTCAAAAGA GCTGCAGTAA ACACCCCCGT CAGCGGGACC 
CCGGTGTCTG GCGGTCCTTC CTCATCCACC CTGCTGGGGC CCTTGAATCT GCACATGTAC 
AGCAGCCAAA GTCTGTTGGC GGGGGAGAGA CCTCTGATTG GCCCCTGCAT CGAGGAGGAC 
GAGCTGGAGC ATGAGACCAG TAGTCAGCCC TCTATGACTA CAGAGAGTTC GGGTTCGTCG 
TCTCACTGTC TGTCTGGCAG TGGCTCTAGC GGATCAGACA GCGGCTGTGT GTCCAGTCAC 
CTTCCTGAGG CTTTGTCTGA GGAGCCCAGC TCACCTTGTG ACTCCTCGTG TTATTCCTCC 
ATCACCTCTC CCACGCAGGA GAAGCCCTGC TTTAACAGCC AGTATATCTC AGCGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301620.1
CDS	167..8026
Translation	MDSESLDSCIQSTLSALYPPFEATAATVLCQVLDVVETTYRGDGLRYIIDFLVPAKHILQSIQQDACFPYCGFLFRHEGWPLCVHEKVIIQLSSLDWRVLRPSDFYLQVTPSAKSPPRIKVKCLAVTGQHAEELDVPELAYTSLFTMDWLDSINQERSVVRRAALEHCLLSADNDIFRVPWEDIVHPEFISRPSKVAEQSESRRVAKEVANGDGRDSDVEQDNNPEDIQCRIFYDTSTNHSGDMDCQSKEVKLLPVLSEINKDISGRSSSSTADDSEGEYVELADISLPRFSPQKGSLTQAISMNYRNLHKPQTPVSTQLKTKPDESLLKNGACSQSMVCAMMIEESLSDTYQTVILTPTAPAEAERPLQQVDMSEPACAAGTASFDSVSEHESERELLSQHSDCEDASLSDISTINISQTDIKPINTSQLDNNVVCKSQDSEPYSTSQLDKNLVNTSQTDTESLSMSQLDDNLDCTSHPDVNQKPSSMSQLELEPLTVSQLDSKLISTSQPDSTSLDVEPVSTSNVDNKPVSTSQPDSTLQLVNEPVKTSQDGEPVSRFHVDHKPVSTLQPDSQPVITSVNEPFRTSQPDNKSVSTTQLDSKPVSVSQHNKQESKSVNTSVDNEPVKTSQPDTESLRISQFDSKPVSNSQLGSKQVSIKQLVNEIFSTPQPDNKSVSTTQLERKLVCGSQDNKQESKSVNTLVDSEPVKTSQTDTELLRISQFDSKPVSNSQLDGKQVSISQLVNEIFSTPQPDNKSVSPTQLDSKLVSTLQDKQESKSVNNSIDNEPVKTSQADTEPLKRSQFDSNPVSNSQLDGKQVSISQLVNEIFSTPQPDNKSVSTTQLDGNIDRTSQLDSKQESVSQTDSKPVSISPDNEPVKTSQPQLESTLHPNSKQASMSQLVNEHIQTSQPDLKPVSIAQLYSEDAKPDGYADVKPITTELDNGLVSTSQPDNQPVDTPQAECTTVNITLPEGNPNDTSYIVNLPLSTSQPNSKTVHVEQADINVINTSQPETEMLSCVLERENSEQVSPAYIELIVSDPYAEVRCESKELRVNCDLEGSCGSQNNRASLAETVAESVSSQNQIDQTGNSLYQIHKPIHTPSEQMHDLPTIKEDHQTFSSSSHNTNESVGDLSPIAEEDGGSVTAALYSEHVALVSVTTVPGESPVTLISQGQVGTESPEDRINQTEVELQTKEERAVQNVAGMPPCKGAEMVEKKSSSEAEIKTDENDVVAVCRQEEVETSQTGLVDATPKSDVQVQEEVSSPVETDEKKQTSVQEKGVEEEEVKAKTVREQEAIVTSNSENRVQSACSEVLDPASDVPNTNGHMHAQDTPTTELKGQITKEEDGKGAEEKRQEDEIASSVNGSQKEHQQQADMDSSFHQQEEQACSQKVDSTVCHLSAKTESEDPSAVPSPQPPVDHRAQPVKSKAHDINPDVLSSGVLCLTGTRDKSGHALVTVTMRNTVWLNPNCNSGELVRIMVYFCSTLRKEVSALGLTVLVDARRCSPVPALFKAFNILQEAMPGCIHTVLLLADRDLALRMEKTSSIQVELLTSLKSLYKHVDVSQLPTEFEGTFPYAHSSWVSFRMRLEQLTSHCEDAVSLLQNTISKLESTVLPPTAEEAQLLLCKYRELMRTVLEDSRLVRLQLEGGAALSRLRKEETSVSLTEDYRDAIESAGRLYNQVDELVHRLVMLSNKCTQELEFIMEFKSLEEGFKEVSQWIEEVGESRLQTLSELEDSLEQLHHKQSIFREFYTAAYEHCKSGEALLKRLEKWEDVSSAELQVYEVKVRSFWVHLRDFSQRVEDTKDKIDKTVQLYEFFDKAYEWALEGMRHLACVSMEDCGMSEKCQSVITCLETYRSQHPPIPDAHFQEMKDLAGELKSEQGLKQWKFAWSKCQETKQMFEKKLEMALRSRRDSDSKSVRSDSSRRNSDVSTKHQERSSSISFSCRKAFGGAWGRDRLSSHSSTSSTPSSPSGIRLDARDTVRTPTGSPYSIEHRIPHSTPVRSHRLTRSISTDESPQSPHAEGLASTSPSGTSVQTNRRVLRKTQSFDTAGSEPVSQYGTCQRTLSEPARRGNTGVFIKGLEVSSTEVIDRPYSPRLPPIHGWSSIDAHRSGSPAPETRSKGSKLRHIVDEMVTTEREYVRSLRYIIDNYFPEMERAVLPQDLRGKRSVIFGNLEKLVDFHSQYFLKELESCCNHPLRVSHCFLRHQDQFSLYALYSKNKPKSDTLLASHGNNFFKHKQMELGDKMDLASYLLKPIQRMSKYALLLKDLIKEVSEVQEQELTYLRAAAEMVKFQLRHGNDLLAMDAIRDCDVNLKEQGQLVRQDEFTISYGRKKCQRHVFLFEDLVLFSKPKRIEGGLDVYIYKHSFKTADVGMTETSGENSLRFEIWFRRRTSKNQTYILQSSTADIKHAWTCDIARILWQQATRNKEIRMQEMVSMGVGNKPFLDIKPSDAAINDRAIDYIMKGRGARTRASIAVSLFDHSNPFKRAAVNTPVSGTPVSGGPSSSTLLGPLNLHMYSSQSLLAGERPLIGPCIEEDELEHETSSQPSMTTESSGSSSHCLSGSGSSGSDSGCVSSHLPEALSEEPSSPCDSSCYSSITSPTQEKPCFNSQYISAV

Target ORF information:

RefSeq Version	XM_009303345.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (plekhg4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303345.2

ORF Insert Sequence:

ATGGACTCTG AATCTCTGGA CAGCTGTATC CAGAGCACAC TGTCCGCTCT CTACCCTCCA 
TTTGAAGCCA CTGCGGCCAC TGTACTGTGC CAGGTGTTAG ATGTGGTGGA GACAACATAC 
CGTGGAGACG GACTGCGATA CATCATCGAC TTTCTGGTGC CCGCTAAACA CATCCTACAG 
AGCATTCAGC AGGATGCATG CTTCCCATAC TGTGGTTTTC TGTTCCGTCA TGAAGGATGG 
CCACTGTGTG TCCATGAGAA GGTCATAATC CAGCTCTCCT CTTTGGACTG GCGTGTATTA 
CGGCCCAGCG ACTTCTACTT GCAGGTGACA CCATCTGCCA AAAGCCCCCC GCGCATCAAA 
GTGAAGTGTC TGGCTGTTAC CGGGCAGCAT GCGGAGGAGC TGGACGTGCC GGAGCTAGCA 
TATACCTCTC TATTCACCAT GGACTGGCTG GACTCCATCA ACCAGGAGAG GAGTGTGGTC 
AGAAGAGCTG CCCTGGAACA CTGTCTTTTA TCAGCGGATA ATGATATTTT TAGGGTACCG 
TGGGAGGATA TTGTTCACCC GGAATTTATC AGCAGACCCT CTAAAGTTGC AGAACAAAGT 
GAAAGTAGAA GGGTTGCCAA AGAAGTTGCA AATGGAGACG GGCGAGACTC TGATGTAGAA 
CAAGACAACA ATCCTGAGGA CATACAATGT AGAATTTTTT ATGACACTTC CACGAACCAT 
TCAGGGGATA TGGATTGTCA GAGCAAGGAA GTTAAGCTCC TCCCTGTTCT TAGTGAGATT 
AATAAAGATA TCAGTGGGAG GAGCTCTAGC AGCACTGCGG ACGATTCAGA GGGCGAATAT 
GTGGAGCTAG CTGATATCTC CTTACCACGC TTTTCCCCAC AAAAGGGCTC TCTTACTCAA 
GCCATTAGCA TGAACTATAG AAATCTACAT AAACCTCAGA CTCCTGTTTC CACTCAGCTT 
AAAACCAAAC CAGATGAGAG TCTTTTGAAG AATGGTGCAT GCTCTCAAAG CATGGTGTGT 
GCCATGATGA TCGAGGAAAG CCTGAGTGAT ACCTACCAAA CAGTGATCCT TACTCCCACT 
GCTCCTGCAG AGGCAGAGCG TCCGCTCCAG CAGGTGGACA TGTCTGAACC CGCCTGTGCA 
GCAGGTACAG CTTCCTTTGA CAGTGTGTCT GAGCATGAGT CTGAGAGAGA GCTATTATCT 
CAACACTCTG ACTGTGAGGA TGCTTCACTA TCTGATATAT CAACAATTAA CATATCGCAA 
ACAGACATTA AACCAATCAA CACATCACAA CTAGACAACA ATGTAGTCTG CAAATCACAA 
GACAGCGAAC CATACAGTAC ATCACAATTA GACAAGAATC TAGTGAACAC ATCACAAACA 
GATACTGAAT CATTGAGCAT GTCTCAATTA GACGATAATT TGGACTGCAC ATCACATCCA 
GATGTTAACC AGAAACCATC AAGTATGTCT CAACTAGAAC TTGAACCACT AACCGTATCT 
CAGCTTGACA GCAAGCTAAT CAGCACATCA CAACCTGATA GCACATCACT AGATGTAGAA 
CCAGTTAGCA CATCTAATGT AGACAACAAA CCAGTCAGCA CATCACAACC GGACAGCACA 
TTACAGTTGG TCAATGAACC AGTCAAGACA TCACAAGATG GTGAACCAGT AAGCAGATTT 
CATGTAGACC ACAAACCAGT AAGCACATTA CAACCAGACA GCCAACCAGT CATCACATCA 
GTCAATGAGC CATTTAGGAC ATCACAACCA GACAATAAAT CAGTAAGCAC AACTCAACTA 
GACAGCAAAC CAGTTAGTGT ATCACAACAC AACAAACAAG AAAGCAAATC TGTCAACACA 
TCTGTTGACA ATGAGCCAGT CAAGACATCA CAGCCAGACA CTGAATCACT AAGAATATCT 
CAATTTGACA GCAAACCAGT CAGCAACTCA CAACTAGGCA GCAAACAAGT CAGCATTAAA 
CAACTAGTCA ATGAGATATT TAGTACACCA CAACCAGACA ATAAATCAGT GAGCACAACT 
CAACTAGAAA GAAAACTAGT TTGCGGATCA CAAGACAACA AACAAGAAAG CAAATCTGTC 
AACACATTAG TAGACAGTGA GCCAGTCAAG ACATCACAGA CAGACACAGA ACTGCTAAGA 
ATATCACAGT TTGATAGCAA ACCAGTCAGC AACTCACAAC TAGATGGCAA ACAAGTCAGC 
ATTTCACAGC TAGTCAATGA GATATTTAGT ACACCACAAC CAGACAATAA ATCAGTAAGC 
CCAACTCAAC TAGACAGTAA GCTAGTTAGC ACATTACAAG ACAAACAAGA AAGCAAATCT 
GTCAATAATT CAATAGACAA TGAGCCAGTC AAGACATCAC AGGCAGACAC AGAACCGCTA 
AAAAGGTCTC AGTTTGACAG CAACCCAGTC AGCAACTCAC AGCTAGATGG CAAACAAGTC 
AGCATTTCAC AACTAGTCAA TGAGATATTT AGTACACCAC AACCAGACAA TAAATCAGTG 
AGCACAACTC AACTAGATGG CAACATAGAT AGAACATCAC AATTAGACAG CAAACAAGAA 
AGTGTCTCTC AAACAGACAG CAAACCAGTC AGCATATCAC CAGACAATGA GCCAGTGAAG 
ACATCACAAC CACAACTAGA AAGCACATTA CACCCAAACA GCAAACAAGC AAGCATGTCA 
CAACTAGTCA ATGAACACAT CCAGACCTCA CAGCCAGACC TAAAACCAGT AAGCATTGCT 
CAACTCTACA GTGAAGATGC TAAACCAGAT GGATACGCTG ATGTCAAACC AATAACCACT 
GAGCTAGACA ATGGACTAGT GAGCACATCA CAACCAGATA ACCAACCAGT TGACACACCA 
CAAGCAGAAT GTACAACAGT TAACATAACC TTACCAGAGG GCAACCCCAA TGACACATCA 
TATATTGTCA ACCTGCCGCT AAGTACATCA CAACCAAACA GCAAAACAGT TCATGTGGAG 
CAAGCAGACA TAAATGTAAT TAATACATCA CAGCCAGAGA CTGAAATGTT ATCTTGTGTT 
TTGGAAAGGG AGAACTCAGA ACAGGTTTCT CCAGCTTATA TTGAACTCAT TGTGTCAGAT 
CCTTATGCAG AAGTCAGATG TGAATCTAAA GAGCTGAGAG TTAATTGTGA TCTTGAAGGC 
AGTTGTGGTT CACAGAACAA CAGAGCGTCA CTTGCCGAGA CAGTAGCTGA GAGTGTGTCT 
TCGCAAAATC AGATTGATCA AACAGGCAAT AGCCTGTATC AGATACACAA ACCCATCCAT 
ACACCATCTG AGCAAATGCA TGATCTTCCT ACCATAAAAG AAGACCATCA GACATTCAGT 
AGCAGTTCTC ATAACACGAA TGAATCTGTT GGTGATTTAA GTCCCATTGC AGAAGAAGAT 
GGTGGGTCTG TTACAGCTGC TCTGTATAGT GAGCATGTAG CCTTAGTTTC TGTCACCACA 
GTGCCTGGGG AGAGTCCAGT TACACTCATT TCACAGGGTC AGGTGGGGAC AGAGTCTCCT 
GAGGACAGAA TCAACCAGAC AGAGGTGGAA CTTCAGACAA AGGAAGAACG TGCAGTACAA 
AACGTAGCTG GAATGCCACC TTGTAAGGGG GCTGAAATGG TAGAAAAGAA AAGCAGTAGC 
GAGGCAGAAA TCAAAACTGA TGAGAATGAT GTAGTGGCAG TGTGTAGGCA AGAGGAAGTT 
GAGACTTCAC AAACAGGTCT AGTAGATGCT ACGCCCAAGT CTGACGTGCA AGTTCAGGAG 
GAGGTCAGCT CCCCTGTAGA AACAGATGAG AAGAAACAAA CCAGTGTTCA AGAAAAAGGG 
GTGGAGGAAG AGGAGGTCAA GGCCAAAACT GTGAGGGAGC AAGAGGCCAT AGTGACCAGC 
AATTCTGAAA ATAGAGTCCA GTCAGCATGC TCTGAAGTGC TTGATCCAGC CTCTGATGTC 
CCGAACACAA ATGGACACAT GCATGCTCAG GACACTCCTA CCACTGAACT AAAAGGGCAA 
ATAACAAAGG AGGAGGATGG AAAAGGGGCA GAGGAGAAAA GGCAAGAGGA TGAGATTGCT 
TCTTCTGTCA ATGGCTCTCA AAAAGAGCAT CAGCAGCAAG CAGACATGGA CTCCAGCTTC 
CATCAGCAGG AAGAGCAAGC ATGCAGTCAG AAAGTAGACA GCACTGTTTG CCACCTGTCT 
GCTAAAACTG AGAGTGAAGA CCCCTCAGCA GTTCCTTCAC CACAGCCACC AGTCGATCAT 
AGAGCACAAC CAGTCAAGAG CAAGGCCCAT GACATAAATC CTGATGTCCT AAGCTCAGGT 
GTGCTCTGCC TGACTGGAAC AAGAGATAAA TCAGGCCATG CCTTGGTCAC TGTGACAATG 
AGAAACACTG TCTGGCTGAA TCCAAACTGC AACAGTGGAG AGCTAGTGCG AATCATGGTG 
TATTTCTGCA GTACACTCAG GAAGGAAGTA AGTGCTTTGG GATTGACTGT CCTGGTGGAT 
GCCCGCAGGT GTTCACCTGT TCCCGCCCTT TTCAAAGCCT TCAACATCCT CCAGGAAGCC 
ATGCCGGGAT GCATTCATAC TGTACTGCTG CTGGCCGACA GAGATCTGGC TCTACGCATG 
GAGAAAACCT CTTCTATACA GGTGGAGCTG CTGACATCAC TCAAGTCTCT CTATAAACAT 
GTGGACGTTT CTCAGCTTCC CACTGAGTTT GAAGGCACTT TCCCTTATGC ACACAGCAGC 
TGGGTCAGCT TTAGAATGAG GTTGGAGCAG TTGACCAGTC ATTGTGAAGA TGCTGTGAGC 
CTGCTTCAGA ACACCATAAG CAAACTGGAG TCCACAGTGC TGCCCCCTAC AGCAGAGGAG 
GCTCAACTCT TACTGTGTAA GTACAGAGAG CTGATGAGGA CGGTTCTGGA GGACAGCAGG 
CTGGTGCGAC TACAGTTGGA GGGAGGAGCA GCTCTGTCCC GTTTACGAAA AGAAGAGACC 
AGCGTTAGTC TCACAGAGGA CTACAGAGAT GCCATTGAAA GTGCTGGCCG TTTGTATAAC 
CAAGTGGACG AGCTGGTTCA CAGACTGGTG ATGCTGTCCA ATAAATGCAC ACAGGAACTG 
GAATTCATCA TGGAGTTTAA GAGCCTGGAG GAGGGATTCA AAGAGGTTAG TCAGTGGATA 
GAAGAGGTGG GAGAGTCTCG TCTGCAGACG CTGAGCGAGC TGGAGGATTC CTTGGAGCAG 
TTACATCATA AGCAGAGCAT CTTCAGAGAA TTTTACACAG CTGCATATGA ACACTGTAAG 
AGTGGTGAAG CTCTCCTCAA GCGTTTAGAG AAGTGGGAAG ACGTCTCCTC TGCAGAGCTG 
CAAGTGTATG AGGTGAAAGT ACGTTCATTC TGGGTGCATC TGCGGGATTT CTCTCAGCGA 
GTGGAGGACA CCAAGGATAA AATAGACAAG ACTGTTCAAC TCTATGAGTT CTTTGATAAG 
GCATATGAAT GGGCTCTAGA GGGCATGCGT CATTTGGCGT GTGTCAGTAT GGAGGATTGT 
GGCATGTCTG AAAAGTGTCA GAGCGTGATC ACGTGTTTGG AGACTTACCG CAGTCAGCAC 
CCCCCGATCC CTGATGCACA CTTCCAGGAG ATGAAGGACC TCGCTGGGGA ACTGAAAAGC 
GAACAGGGCC TCAAGCAGTG GAAGTTTGCC TGGTCCAAGT GTCAGGAGAC CAAGCAGATG 
TTTGAAAAGA AGCTGGAGAT GGCCCTGCGT TCCCGCCGTG ACAGCGACTC CAAGTCAGTA 
AGGAGTGACT CTTCTCGTCG TAACTCAGAT GTTAGCACTA AACACCAAGA GCGTAGCAGT 
AGCATCTCCT TCTCATGTCG GAAAGCGTTC GGCGGAGCCT GGGGTCGAGA TAGACTTTCC 
TCACATTCTA GCACATCCTC AACTCCCAGT AGCCCTTCAG GCATTCGTCT GGATGCCCGT 
GACACTGTCC GAACTCCAAC AGGAAGTCCA TACAGCATTG AGCACAGAAT CCCCCACAGC 
ACCCCTGTCA GATCCCACAG GCTAACACGT AGCATCTCCA CAGATGAGTC TCCTCAGAGC 
CCACATGCAG AGGGGCTAGC GTCCACAAGC CCAAGCGGCA CTTCTGTTCA AACAAACCGC 
AGGGTTTTGC GGAAGACCCA GAGCTTCGAT ACAGCTGGCA GTGAGCCTGT GTCACAGTAC 
GGGACCTGTC AGAGGACTTT GAGTGAGCCT GCACGCAGAG GAAACACAGG GGTGTTCATT 
AAGGGTCTGG AGGTGAGCAG CACTGAAGTG ATTGATAGGC CGTACAGTCC ACGACTACCA 
CCCATCCATG GATGGTCTTC AATTGATGCA CACCGCAGCG GGAGTCCTGC TCCAGAAACG 
CGCAGCAAGG GCAGCAAGTT ACGACACATT GTAGATGAGA TGGTGACAAC AGAGCGGGAA 
TATGTGCGTT CCCTGCGGTA TATCATTGAC AACTATTTCC CTGAGATGGA GCGCGCTGTC 
CTGCCGCAGG ATCTGCGGGG AAAGCGGAGC GTCATATTTG GAAATCTGGA GAAACTGGTG 
GACTTTCATA GTCAGTATTT CCTAAAAGAG CTGGAGAGCT GCTGTAATCA TCCTCTACGC 
GTCAGCCACT GTTTCCTACG ACATCAAGAC CAGTTTAGCC TCTATGCATT ATACAGCAAG 
AACAAGCCAA AATCAGACAC ACTCCTGGCC AGCCATGGAA ACAACTTCTT CAAGCATAAG 
CAGATGGAGC TGGGAGATAA GATGGACCTG GCATCGTACC TGCTGAAGCC CATCCAGCGC 
ATGAGTAAAT ACGCTCTTCT GCTGAAAGAC CTGATCAAAG AAGTGAGCGA GGTCCAGGAG 
CAGGAACTTA CATACCTGCG AGCCGCAGCA GAGATGGTCA AGTTTCAGCT CCGCCATGGC 
AATGATCTAC TCGCCATGGA CGCTATCAGA GATTGTGATG TGAACCTAAA AGAGCAAGGA 
CAGTTGGTTC GTCAAGATGA GTTCACGATT TCATATGGCA GAAAGAAGTG CCAGAGACAC 
GTGTTCCTAT TTGAGGACCT AGTGCTCTTC AGCAAACCCA AGCGCATCGA GGGTGGACTA 
GATGTTTACA TATACAAGCA TTCTTTTAAG ACGGCTGATG TGGGTATGAC AGAGACGTCA 
GGTGAGAACA GTCTGAGGTT TGAAATTTGG TTTCGGAGGA GAACTTCAAA GAACCAGACC 
TACATTCTCC AGTCCAGCAC TGCTGACATC AAACATGCCT GGACTTGCGA CATCGCACGA 
ATACTGTGGC AGCAGGCCAC GCGAAACAAA GAAATTCGAA TGCAGGAGAT GGTCTCAATG 
GGTGTGGGCA ATAAACCCTT CCTGGACATT AAACCCAGTG ATGCTGCAAT CAACGACAGA 
GCCATCGACT ACATTATGAA GGGCAGAGGA GCTAGAACGA GAGCCTCCAT TGCTGTTTCT 
CTGTTTGACC ACTCAAACCC ATTCAAAAGA GCTGCAGTAA ACACCCCCGT CAGCGGGACC 
CCGGTGTCTG GCGGTCCTTC CTCATCCACC CTGCTGGGGC CCTTGAATCT GCACATGTAC 
AGCAGCCAAA GTCTGTTGGC GGGGGAGAGA CCTCTGATTG GCCCCTGCAT CGAGGAGGAC 
GAGCTGGAGC ATGAGACCAG TAGTCAGCCC TCTATGACTA CAGAGAGTTC GGGTTCGTCG 
TCTCACTGTC TGTCTGGCAG TGGCTCTAGC GGATCAGACA GCGGCTGTGT GTCCAGTCAC 
CTTCCTGAGG CTTTGTCTGA GGAGCCCAGC TCACCTTGTG ACTCCTCGTG TTATTCCTCC 
ATCACCTCTC CCACGCAGGA GAAGCCCTGC TTTAACAGCC AGTATATCTC AGCGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	plekhg4
Entrez Gene ID	326099
Full Name	pleckstrin homology domain containing, family G (with RhoGef domain) member 4
Synonyms	id:ibd5125,wu:fd99e06
General protein information	Preferred Names pleckstrin homology domain containing, family G (with RhoGef domain) member 4 Names puratrophin-1 pleckstrin homology domain-containing family G member 4B uncharacterized protein plekhg4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Rattus norvegicus (Norway rat)	Plekhg4	NP_001129342.1
Pan troglodytes (chimpanzee)	PLEKHG4	XP_003315221.1
Homo sapiens (human)	PLEKHG4	NP_001123199.1
Gallus gallus (chicken)	PLEKHG4	XP_004944159.1
Danio rerio (zebrafish)	plekhg4	XP_005169064.1
Xenopus tropicalis (tropical clawed frog)	plekhg4	NP_001128280.1
Bos taurus (cattle)	PLEKHG4	XP_005218907.1
	PLEKHG4	XP_001087633.1
Canis lupus familiaris (dog)	PLEKHG4	XP_005620880.1
Mus musculus (house mouse)	Plekhg4	NP_001074802.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

plekhg4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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