PLEKHG4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLEKHG4
Entrez Gene ID	787892
Full Name	pleckstrin homology and RhoGEF domain containing G4
General protein information	Preferred Names pleckstrin homology and RhoGEF domain containing G4 Names puratrophin-1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015475823.1	XP_015331309.1	puratrophin-1
XM_015475823.2	XP_015331309.2	puratrophin-1 isoform X1
XM_005218850.2	XP_005218907.1	puratrophin-1
XM_015467369.1	XP_015322855.1	puratrophin-1
XM_005195262.2	XP_005195319.1	puratrophin-1
XM_024978976.1	XP_024834744.1	puratrophin-1 isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Plekhg4	NP_001129342.1
Pan troglodytes (chimpanzee)	PLEKHG4	XP_003315221.1
Homo sapiens (human)	PLEKHG4	NP_001123199.1
Gallus gallus (chicken)	PLEKHG4	XP_004944159.1
Danio rerio (zebrafish)	plekhg4	XP_005169064.1
Xenopus tropicalis (tropical clawed frog)	plekhg4	NP_001128280.1
Bos taurus (cattle)	PLEKHG4	XP_005218907.1
	PLEKHG4	XP_001087633.1
Canis lupus familiaris (dog)	PLEKHG4	XP_005620880.1
Mus musculus (house mouse)	Plekhg4	NP_001074802.1

Gene Ontology

The following PLEKHG4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLEKHG4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa225484	XM_015475823.2 Latest version!	Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa225485	XM_024978976.1 Latest version!	Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa09337	XM_015467369.1 Latest version!	Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa09337	XM_005195262.2 Latest version!	Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa09337	XM_005218850.2 Latest version!	Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa09337	XM_015475823.1 Latest version!	Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLEKHG4 ( XM_015475823.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015331309.2 Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa225484
Clone ID Related Accession (Same CDS sequence)	XM_015475823.2
Accession Version	XM_015475823.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	puratrophin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475823.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGGC CCCTGGAGGA TGGGGATGAG TCTCCAGAGT CCCAGGGCCA TGCCACCGAC 
TGGAGATTTG CTGTGTGCAG TTTCAGAGAT GCCTGGGATG AGGGGGAACC TGCTCCCCAG 
ATGCAGGCTA AGAACCCCCA TCCTCCAAGC CCACCAGCAG GGGCAGCCCA GGGGGAGGAG 
CTCCAGGGCA GCCCTCTGCC TGGTGAACTT CAGTCACCCA CAGGACAGAT GGTTGCAGAT 
GAGTCGGGGC ATGGCCAGAA GGGCACATTA AGAGGATCTT CTGTCCACAT GAAGGAACCA 
GCCTCCTCTG GAGTGAAGAT CCTCCTAAGT CCAATGTCTT CCCACCTTAG TCTGGCACAG 
GGTGAGAATG ACAGCCAAGG AGGAGACTTG GCAGGGGACT CAGTTTCAGG CAGGGTCATG 
CCAGTCCGCT TGGACCCTGA AGTGTTGGAC AGTGACCCTG TGAACCATGG AGGTCTTTTA 
TCTGAGACAT CGTCAGAGCT ACTGGAGGCA GACTCCCACA GATCCAGACT CTTGAAGTCC 
ACTGACTACC TCCTGGCCCA AGACATTGCC TGGGAGCTAC TTGCCAGTGG CATGGCTGCC 
CTGCCAGGGA CTCGAGATGT AGAAGGCCGG GCAGTGCTGC TTCTGTGTGC CCATAACTCA 
GCTTGGAATC ACCCCAAGTG CAACAGCCAT GAGCTCGTCC GCCTCCTGCT CTACTTGCAA 
AGCATCCCCA GGCCCAAAGT ACAGACCTTG GGACTGACCG TGCTAGTGGA CGCCCGGATT 
TGTTCCCCAA GCTCTTCCAT ATTCTGGGGG CTCAGCCAAC TGCAAGAAGC AGTCCCAGGA 
TCAGTGCACC GTGTGCTGCT GGTGGGAAAG CTGCCGGAGG AAGTGCCTGC AGGGCTTCAG 
ATAGAGCATC TATCCTCTCA CCAAAGCCTG CTGACCCACA TCTCCACCTC AGAGTTGCTC 
ACTTCACTAG GAGGAGGCCT GCCTTACTGT CACCAAGCTT GGCTGGACTT CCGGATGCGT 
CTGGAAGCCC TCCTGCAGAG CTGCCAGGTG GTTTGTGCCC TGCTTCAGGG AGCCATTGAG 
AGCATGAAGG CTGTGCCTCA GCCTGTGGAG TCTGGGGAAG TCGGTCAACT GCTGCAGCAG 
GCACGGGTCC TGATGAAGCA TGTGCTAGAC TCTCCACGGT TGGCATGGCT ACAATGCCAG 
GGAGCCTTGG AGCTGACATG GCTAAAGCAA GAGGTCCCAG AGGTGACCCT GAGCCCAGAC 
TACAGGTCAG CAGTGGACGA GGTTGAGGAG CTGTATGGCC GCGTGGATGG ATTGTTGCAC 
CAACTGACAC TGCAGGGTAA CCGCCGAATA CAAGCACTGG AGTTGGTCCA GACGCTGGAG 
GCCCAGGAGG GCGAGCTGCA CCAGATGGAA GTGTGGCTTC AGCAGGTGGG CTGGCCAGCA 
CTTGAGGAGC CCAGGGAGCC TTCACTAGAC GTGCTGCTTC AGGCCCAAGG CCCTTTTCGG 
GAGCTGGACC AGATTGCTCA GGAACAGGTC AGGCGAGGGG AGAAATGTCT GCAGCCACTG 
GTTGGCTGTG ATGCGGCCGA ACTGGGCCCC TTTGGGGCCC GCTTTCTGAC CCTGCAAACC 
CAGCTGGCCG ACTTCTCTAG GGCTTTGGCC CAGCGGCGGC AGCGACTGGC AGATGCTGAG 
AGACTCTTGC AGTTCTTCAA GCAGGCCTTG ACCTGGGCCG AGGAGGGGCG GCAAGTGTTG 
GCAGAGCTGG AACAGGAGCA CCCAGGGGTT GTGCTGCAGC GGCTGCAGCT GCATTGGACC 
AAGTACCCGG ACTTGCCTCC TGCCCACTTC CGAAAGATGT GGGCTTTAGC CACAGGGCTG 
GGATCTGAGG CCATTCGCCA TGAGTGCCGC GGGGCCTGGG CGCAGTGCCA GGACACCTGG 
CTGGCCCTGG ATCAGAAGTT AGAAGCTGCT CTGAAGTCAC CCCCAACGGG TAGCACAGCT 
AGCCTGTGTG TCAGCCGGGT CCCAGCGGTA CCGGCTGTCC CTCCCCTGAG GAAAGCCTAT 
AGCCTTGATC GGAATCTGGG ATTGAGTCTT AGAGAACCTG CCCACCAGTG CCACCATGAG 
GCCATTGTAC CTGCCTGCCA CAGACCAGAG GCTGGAGGTG GTGCCCAGTC AGGGTCATGC 
CCCACCGTGC CTCCTCCAGG GAGCTGTGAC CCCAGGAGCC CCAACAGACT TCAGCTGGTG 
CTGGCGGAGA TGGTGGCTAC GGAGCGGGAG TATGTCCGTG CCCTGGACTA CACCATGGAG 
AACTACTTCC CTGAGCTGGA TCGCCCTGAT GTGCCCCAGG GCCTCCGTGG CCAGCGTGCC 
CAACTCTTTG GCAACCTGGA GAAGCTGTGG GACTTCCACC GCCATTTTTT CTTGCGTGAG 
CTGGAGGCCT GTACCCAGCA CCCGCCCCGG GTGGCCTATG CCTTCCTGCG CCATAGGGTG 
CAGTTTGGGA TGTACGCACT CTACAGCAAG AATAAACCGC GCTCCGATGC CCTGATGACC 
AGCTATGGTC ACGTCTTCTT CAGGGACAAG CAGCAGGCAC TGGGGGACCA CTTGGACTTG 
GCCTCCTACC TGCTGAAGCC CATCCAGCGC ATGAGCAAGT ATGCGCTGCT GCTGCAGGAG 
CTGGCACGGG CCTGCGGGGG ACCCGCACAG GAGCTGAGTG CCCTGCGGGC TGCCCAGAGC 
CTCGTGCACT TCCAGCTGCG ACACGGCAAT GACTTGCTAG CCATGGATGC CATCCAGGGC 
TGTGACGTCA ACCTCAAGGA ACAGGGCCAG TTGGTCCGGC AGGATGAGTT CACAGTCCGC 
TGTGGGCGCC ACAAATCCCT GCGTCGCATT TTCCTCTTTG AGGAGCTGTT GCTCTTCAGC 
AAGCCTCGCC GAGGACCCAC AGGCATTGAC ATGTTTACCT ACAAGCGCTC CTTCAAGATG 
GCAGACCTTG GCCTCACTGA GTGCTGTGGG GACAACAACT TGCGCTTTGA GATCTGGTTT 
CGCCGTCGCA AGGCTAGGGA CACCTTTGTG CTACAAGCTG CCAGCTTGGC CACCAAGCAG 
GCCTGGACAG CTGACATCTC CCGCCTGCTC TGGAGGCAGG CCGTCCACAA CAAGGAGGTG 
CGCATGGCTG AGATGGTGTC TATGGGTGTG GGGAACAAGG CCTTCCGGGA CATCGCACCC 
AGCGAAGAAG CTATCAACGA CCGCACCGTC AACTATATCC TGAAGTGCCA AGCAGTTCGC 
TCTCGGGCCT CCATTGCTGT GGCTCCTCTT GACTCTGACA GCCCCTACCT GGGGGCCTCA 
GGCACCCTTC CTGGAGATCC TGCCTCTTGC TCTGTACTGG GGTCCCTCAA CCTGCATCTG 
CACAGAGACC CAGCTCTTCT GGGGTTCCGC TGGCCCCTGT ATTCCACCAG TTTCCCAGAG 
GAAGCAGCAC TGGAGACTGA TGCCGAACTG GGCAGCCAGC CATCTTTGAC TCCTGAGGAC 
TCAGAGGTTT CCTCCCAATG CCCATCAGCC AGTGGCTCCA GTGGCTCAGA CAGCAGCTGT 
GTGTCAGGGC AGATCCTGGG CAAGGGCCTG GAGGACTTGT CCTACGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015331309.2
CDS	345..3935
Translation	MEGPLEDGDESPESQGHATDWRFAVCSFRDAWDEGEPAPQMQAKNPHPPSPPAGAAQGEELQGSPLPGELQSPTGQMVADESGHGQKGTLRGSSVHMKEPASSGVKILLSPMSSHLSLAQGENDSQGGDLAGDSVSGRVMPVRLDPEVLDSDPVNHGGLLSETSSELLEADSHRSRLLKSTDYLLAQDIAWELLASGMAALPGTRDVEGRAVLLLCAHNSAWNHPKCNSHELVRLLLYLQSIPRPKVQTLGLTVLVDARICSPSSSIFWGLSQLQEAVPGSVHRVLLVGKLPEEVPAGLQIEHLSSHQSLLTHISTSELLTSLGGGLPYCHQAWLDFRMRLEALLQSCQVVCALLQGAIESMKAVPQPVESGEVGQLLQQARVLMKHVLDSPRLAWLQCQGALELTWLKQEVPEVTLSPDYRSAVDEVEELYGRVDGLLHQLTLQGNRRIQALELVQTLEAQEGELHQMEVWLQQVGWPALEEPREPSLDVLLQAQGPFRELDQIAQEQVRRGEKCLQPLVGCDAAELGPFGARFLTLQTQLADFSRALAQRRQRLADAERLLQFFKQALTWAEEGRQVLAELEQEHPGVVLQRLQLHWTKYPDLPPAHFRKMWALATGLGSEAIRHECRGAWAQCQDTWLALDQKLEAALKSPPTGSTASLCVSRVPAVPAVPPLRKAYSLDRNLGLSLREPAHQCHHEAIVPACHRPEAGGGAQSGSCPTVPPPGSCDPRSPNRLQLVLAEMVATEREYVRALDYTMENYFPELDRPDVPQGLRGQRAQLFGNLEKLWDFHRHFFLRELEACTQHPPRVAYAFLRHRVQFGMYALYSKNKPRSDALMTSYGHVFFRDKQQALGDHLDLASYLLKPIQRMSKYALLLQELARACGGPAQELSALRAAQSLVHFQLRHGNDLLAMDAIQGCDVNLKEQGQLVRQDEFTVRCGRHKSLRRIFLFEELLLFSKPRRGPTGIDMFTYKRSFKMADLGLTECCGDNNLRFEIWFRRRKARDTFVLQAASLATKQAWTADISRLLWRQAVHNKEVRMAEMVSMGVGNKAFRDIAPSEEAINDRTVNYILKCQAVRSRASIAVAPLDSDSPYLGASGTLPGDPASCSVLGSLNLHLHRDPALLGFRWPLYSTSFPEEAALETDAELGSQPSLTPEDSEVSSQCPSASGSSGSDSSCVSGQILGKGLEDLSYV

Target ORF information:

RefSeq Version	XM_015475823.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475823.2

ORF Insert Sequence:

ATGGAAGGGC CCCTGGAGGA TGGGGATGAG TCTCCAGAGT CCCAGGGCCA TGCCACCGAC 
TGGAGATTTG CTGTGTGCAG TTTCAGAGAT GCCTGGGATG AGGGGGAACC TGCTCCCCAG 
ATGCAGGCTA AGAACCCCCA TCCTCCAAGC CCACCAGCAG GGGCAGCCCA GGGGGAGGAG 
CTCCAGGGCA GCCCTCTGCC TGGTGAACTT CAGTCACCCA CAGGACAGAT GGTTGCAGAT 
GAGTCGGGGC ATGGCCAGAA GGGCACATTA AGAGGATCTT CTGTCCACAT GAAGGAACCA 
GCCTCCTCTG GAGTGAAGAT CCTCCTAAGT CCAATGTCTT CCCACCTTAG TCTGGCACAG 
GGTGAGAATG ACAGCCAAGG AGGAGACTTG GCAGGGGACT CAGTTTCAGG CAGGGTCATG 
CCAGTCCGCT TGGACCCTGA AGTGTTGGAC AGTGACCCTG TGAACCATGG AGGTCTTTTA 
TCTGAGACAT CGTCAGAGCT ACTGGAGGCA GACTCCCACA GATCCAGACT CTTGAAGTCC 
ACTGACTACC TCCTGGCCCA AGACATTGCC TGGGAGCTAC TTGCCAGTGG CATGGCTGCC 
CTGCCAGGGA CTCGAGATGT AGAAGGCCGG GCAGTGCTGC TTCTGTGTGC CCATAACTCA 
GCTTGGAATC ACCCCAAGTG CAACAGCCAT GAGCTCGTCC GCCTCCTGCT CTACTTGCAA 
AGCATCCCCA GGCCCAAAGT ACAGACCTTG GGACTGACCG TGCTAGTGGA CGCCCGGATT 
TGTTCCCCAA GCTCTTCCAT ATTCTGGGGG CTCAGCCAAC TGCAAGAAGC AGTCCCAGGA 
TCAGTGCACC GTGTGCTGCT GGTGGGAAAG CTGCCGGAGG AAGTGCCTGC AGGGCTTCAG 
ATAGAGCATC TATCCTCTCA CCAAAGCCTG CTGACCCACA TCTCCACCTC AGAGTTGCTC 
ACTTCACTAG GAGGAGGCCT GCCTTACTGT CACCAAGCTT GGCTGGACTT CCGGATGCGT 
CTGGAAGCCC TCCTGCAGAG CTGCCAGGTG GTTTGTGCCC TGCTTCAGGG AGCCATTGAG 
AGCATGAAGG CTGTGCCTCA GCCTGTGGAG TCTGGGGAAG TCGGTCAACT GCTGCAGCAG 
GCACGGGTCC TGATGAAGCA TGTGCTAGAC TCTCCACGGT TGGCATGGCT ACAATGCCAG 
GGAGCCTTGG AGCTGACATG GCTAAAGCAA GAGGTCCCAG AGGTGACCCT GAGCCCAGAC 
TACAGGTCAG CAGTGGACGA GGTTGAGGAG CTGTATGGCC GCGTGGATGG ATTGTTGCAC 
CAACTGACAC TGCAGGGTAA CCGCCGAATA CAAGCACTGG AGTTGGTCCA GACGCTGGAG 
GCCCAGGAGG GCGAGCTGCA CCAGATGGAA GTGTGGCTTC AGCAGGTGGG CTGGCCAGCA 
CTTGAGGAGC CCAGGGAGCC TTCACTAGAC GTGCTGCTTC AGGCCCAAGG CCCTTTTCGG 
GAGCTGGACC AGATTGCTCA GGAACAGGTC AGGCGAGGGG AGAAATGTCT GCAGCCACTG 
GTTGGCTGTG ATGCGGCCGA ACTGGGCCCC TTTGGGGCCC GCTTTCTGAC CCTGCAAACC 
CAGCTGGCCG ACTTCTCTAG GGCTTTGGCC CAGCGGCGGC AGCGACTGGC AGATGCTGAG 
AGACTCTTGC AGTTCTTCAA GCAGGCCTTG ACCTGGGCCG AGGAGGGGCG GCAAGTGTTG 
GCAGAGCTGG AACAGGAGCA CCCAGGGGTT GTGCTGCAGC GGCTGCAGCT GCATTGGACC 
AAGTACCCGG ACTTGCCTCC TGCCCACTTC CGAAAGATGT GGGCTTTAGC CACAGGGCTG 
GGATCTGAGG CCATTCGCCA TGAGTGCCGC GGGGCCTGGG CGCAGTGCCA GGACACCTGG 
CTGGCCCTGG ATCAGAAGTT AGAAGCTGCT CTGAAGTCAC CCCCAACGGG TAGCACAGCT 
AGCCTGTGTG TCAGCCGGGT CCCAGCGGTA CCGGCTGTCC CTCCCCTGAG GAAAGCCTAT 
AGCCTTGATC GGAATCTGGG ATTGAGTCTT AGAGAACCTG CCCACCAGTG CCACCATGAG 
GCCATTGTAC CTGCCTGCCA CAGACCAGAG GCTGGAGGTG GTGCCCAGTC AGGGTCATGC 
CCCACCGTGC CTCCTCCAGG GAGCTGTGAC CCCAGGAGCC CCAACAGACT TCAGCTGGTG 
CTGGCGGAGA TGGTGGCTAC GGAGCGGGAG TATGTCCGTG CCCTGGACTA CACCATGGAG 
AACTACTTCC CTGAGCTGGA TCGCCCTGAT GTGCCCCAGG GCCTCCGTGG CCAGCGTGCC 
CAACTCTTTG GCAACCTGGA GAAGCTGTGG GACTTCCACC GCCATTTTTT CTTGCGTGAG 
CTGGAGGCCT GTACCCAGCA CCCGCCCCGG GTGGCCTATG CCTTCCTGCG CCATAGGGTG 
CAGTTTGGGA TGTACGCACT CTACAGCAAG AATAAACCGC GCTCCGATGC CCTGATGACC 
AGCTATGGTC ACGTCTTCTT CAGGGACAAG CAGCAGGCAC TGGGGGACCA CTTGGACTTG 
GCCTCCTACC TGCTGAAGCC CATCCAGCGC ATGAGCAAGT ATGCGCTGCT GCTGCAGGAG 
CTGGCACGGG CCTGCGGGGG ACCCGCACAG GAGCTGAGTG CCCTGCGGGC TGCCCAGAGC 
CTCGTGCACT TCCAGCTGCG ACACGGCAAT GACTTGCTAG CCATGGATGC CATCCAGGGC 
TGTGACGTCA ACCTCAAGGA ACAGGGCCAG TTGGTCCGGC AGGATGAGTT CACAGTCCGC 
TGTGGGCGCC ACAAATCCCT GCGTCGCATT TTCCTCTTTG AGGAGCTGTT GCTCTTCAGC 
AAGCCTCGCC GAGGACCCAC AGGCATTGAC ATGTTTACCT ACAAGCGCTC CTTCAAGATG 
GCAGACCTTG GCCTCACTGA GTGCTGTGGG GACAACAACT TGCGCTTTGA GATCTGGTTT 
CGCCGTCGCA AGGCTAGGGA CACCTTTGTG CTACAAGCTG CCAGCTTGGC CACCAAGCAG 
GCCTGGACAG CTGACATCTC CCGCCTGCTC TGGAGGCAGG CCGTCCACAA CAAGGAGGTG 
CGCATGGCTG AGATGGTGTC TATGGGTGTG GGGAACAAGG CCTTCCGGGA CATCGCACCC 
AGCGAAGAAG CTATCAACGA CCGCACCGTC AACTATATCC TGAAGTGCCA AGCAGTTCGC 
TCTCGGGCCT CCATTGCTGT GGCTCCTCTT GACTCTGACA GCCCCTACCT GGGGGCCTCA 
GGCACCCTTC CTGGAGATCC TGCCTCTTGC TCTGTACTGG GGTCCCTCAA CCTGCATCTG 
CACAGAGACC CAGCTCTTCT GGGGTTCCGC TGGCCCCTGT ATTCCACCAG TTTCCCAGAG 
GAAGCAGCAC TGGAGACTGA TGCCGAACTG GGCAGCCAGC CATCTTTGAC TCCTGAGGAC 
TCAGAGGTTT CCTCCCAATG CCCATCAGCC AGTGGCTCCA GTGGCTCAGA CAGCAGCTGT 
GTGTCAGGGC AGATCCTGGG CAAGGGCCTG GAGGACTTGT CCTACGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHG4 ( XM_024978976.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834744.1 Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa225485
Clone ID Related Accession (Same CDS sequence)	XM_024978976.1
Accession Version	XM_024978976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	puratrophin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGGC CCCTGGAGGA TGGGGATGAG TCTCCAGAGT CCCAGGGCCA TGCCACCGAC 
TGGAGATTTG CTGTGTGCAG TTTCAGAGAT GCCTGGGATG AGGGGGAACC TGCTCCCCAG 
ATGCAGGCTA AGAACCCCCA TCCTCCAAGC CCACCAGCAG GGGCAGCCCA GGGGGAGGAG 
CTCCAGGGCA GCCCTCTGCC TGGTGAACTT CAGTCACCCA CAGGACAGAT GGTTGCAGAT 
GAGTCGGGGC ATGGCCAGAA GGGCACATTA AGAGGATCTT CTGTCCACAT GAAGGAACCA 
GCCTCCTCTG GAGTGAAGAT CCTCCTAAGT CCAATGTCTT CCCACCTTAG TCTGGCACAG 
GGTGAGAATG ACAGCCAAGG AGGAGACTTG GCAGGGGACT CAGTTTCAGG CAGGGTCATG 
CCAGTCCGCT TGGACCCTGA AGTGTTGGAC AGTGACCCTG TGAACCATGG AGGTCTTTTA 
TCTGAGACAT CGTCAGAGCT ACTGGAGGCA GACTCCCACA GATCCAGACT CTTGAAGTCC 
ACTGACTACC TCCTGGCCCA AGACATTGCC TGGGAGCTAC TTGCCAGTGG CATGGCTGCC 
CTGCCAGGGA CTCGAGATGT AGAAGGCCGG GCAGTGCTGC TTCTGTGTGC CCATAACTCA 
GCTTGGAATC ACCCCAAGTG CAACAGCCAT GAGCTCGTCC GCCTCCTGCT CTACTTGCAA 
AGCATCCCCA GGCCCAAAGT ACAGACCTTG GGACTGACCG TGCTAGTGGA CGCCCGGATT 
TGTTCCCCAA GCTCTTCCAT ATTCTGGGGG CTCAGCCAAC TGCAAGAAGC AGTCCCAGGA 
TCAGTGCACC GTGTGCTGCT GGTGGGAAAG CTGCCGGAGG AAGTGCCTGC AGGGCTTCAG 
ATAGAGCATC TATCCTCTCA CCAAAGCCTG CTGACCCACA TCTCCACCTC AGAGTTGCTC 
ACTTCACTAG GAGGAGGCCT GCCTTACTGT CACCAAGCTT GGCTGGACTT CCGGATGCGT 
CTGGAAGCCC TCCTGCAGAG CTGCCAGGTG GTTTGTGCCC TGCTTCAGGG AGCCATTGAG 
AGCATGAAGG CTGTGCCTCA GCCTGTGGAG TCTGGGGAAG TCGGTCAACT GCTGCAGCAG 
GCACGGGTCC TGATGAAGCA TGTGCTAGAC TCTCCACGGT TGGCATGGCT ACAATGCCAG 
GGAGCCTTGG AGCTGACATG GCTAAAGCAA GAGGTCCCAG AGGTGACCCT GAGCCCAGAC 
TACAGGTCAG CAGTGGACGA GGTTGAGGAG CTGTATGGCC GCGTGGATGG ATTGTTGCAC 
CAACTGACAC TGCAGGGTAA CCGCCGAATA CAAGCACTGG AGTTGGTCCA GACGCTGGAG 
GCCCAGGAGG GCGAGCTGCA CCAGATGGAA GTGTGGCTTC AGCAGGTGGG CTGGCCAGCA 
CTTGAGGAGC CCAGGGAGCC TTCACTAGAC GTGCTGCTTC AGGCCCAAGG CCCTTTTCGG 
GAGCTGGACC AGATTGCTCA GGAACAGGTC AGGCGAGGGG AGAAATGTCT GCAGCCACTG 
GTTGGCTGTG ATGCGGCCGA ACTGGGCCCC TTTGGGGCCC GCTTTCTGAC CCTGCAAACC 
CAGCTGGCCG ACTTCTCTAG GGCTTTGGCC CAGCGGCGGC AGCGACTGGC AGATGCTGAG 
AGACTCTTGC AGTTCTTCAA GCAGGCCTTG ACCTGGGCCG AGGAGGGGCG GCAAGTGTTG 
GCAGAGCTGG AACAGGAGCA CCCAGGGGTT GTGCTGCAGC GGCTGCAGCT GCATTGGACC 
AAGTACCCGG ACTTGCCTCC TGCCCACTTC CGAAAGATGT GGGCTTTAGC CACAGGGCTG 
GGATCTGAGG CCATTCGCCA TGAGTGCCGC GGGGCCTGGG CGCAGTGCCA GGACACCTGG 
CTGGCCCTGG ATCAGAAGTT AGAAGCTGCT CTGAAGTCAC CCCCAACGGG TAGCACAGCT 
AGCCTGTGTG TCAGCCGGGT CCCAGCGGTA CCGGCTGTCC CTCCCCTGAG GAAAGCCTAT 
AGCCTTGATC GGAATCTGGG ATTGAGTCTT AGAGAACCTG CCCACCAGTG CCACCATGAG 
GCCATTGTAC CTGCCTGCCA CAGACCAGAG GCTGGAGGTG GTGCCCAGTC AGGGTCATGC 
CCCACCGTGC CTCCTCCAGG GAGCTGTGAC CCCAGGAGCC CCAACAGACT TCAGCTGGTG 
CTGGCGGAGA TGGTGGCTAC GGAGCGGGAG TATGTCCGTG CCCTGGACTA CACCATGGAG 
AACTACTTCC CTGAGCTGGA TCGCCCTGAT GTGCCCCAGG GCCTCCGTGG CCAGCGTGCC 
CAACTCTTTG GCAACCTGGA GAAGCTGTGG GACTTCCACC GCCATTTTTT CTTGCGTGAG 
CTGGAGGCCT GTACCCAGCA CCCGCCCCGG GTGGCCTATG CCTTCCTGCG CCATAGGGTG 
CAGTTTGGGA TGTACGCACT CTACAGCAAG AATAAACCGC GCTCCGATGC CCTGATGACC 
AGCTATGGTC ACGTCTTCTT CAGGGACAAG CAGCAGGCAC TGGGGGACCA CTTGGACTTG 
GCCTCCTACC TGCTGAAGCC CATCCAGCGC ATGAGCAAGT ATGCGCTGCT GCTGCAGGAG 
CTGGCACGGG CCTGCGGGGG ACCCGCACAG GAGCTGAGTG CCCTGCGGGC TGCCCAGAGC 
CTCGTGCACT TCCAGCTGCG ACACGGCAAT GACTTGCTAG CCATGGATGC CATCCAGGGC 
TGTGACGTCA ACCTCAAGGA ACAGGGCCAG TTGGTCCGGC AGGATGAGTT CACAGTCCGC 
TGTGGGCGCC ACAAATCCCT GCGTCGCATT TTCCTCTTTG AGGAGCTGTT GCTCTTCAGC 
AAGCCTCGCC GAGGACCCAC AGGCATTGAC ATGTTTACCT ACAAGCGCTC CTTCAAGATG 
GCAGACCTTG GCCTCACTGA GTGCTGTGGG GACAACAACT TGCGCTTTGA GATCTGGTTT 
CGCCGTCGCA AGGCTAGGGA CACCTTTGTG CTACAAGCTG CCAGCTTGGC CACCAAGCAG 
GCCTGGACAG CTGACATCTC CCGCCTGCTC TGGAGGCAGG CCGTCCACAA CAAGGAGGTG 
CGCATGGCTG AGATGGTGTC TATGGGTGTG GGGAACAAGG CCTTCCGGGA CATCGCACCC 
AGCGAAGAAG CTATCAACGA CCGCACCGTC AACTATATCC TGAAGTGCCA AGTTCGCTCT 
CGGGCCTCCA TTGCTGTGGC TCCTCTTGAC TCTGACAGCC CCTACCTGGG GGCCTCAGGC 
ACCCTTCCTG GAGATCCTGC CTCTTGCTCT GTACTGGGGT CCCTCAACCT GCATCTGCAC 
AGAGACCCAG CTCTTCTGGG GTTCCGCTGG CCCCTGTATT CCACCAGTTT CCCAGAGGAA 
GCAGCACTGG AGACTGATGC CGAACTGGGC AGCCAGCCAT CTTTGACTCC TGAGGACTCA 
GAGGTTTCCT CCCAATGCCC ATCAGCCAGT GGCTCCAGTG GCTCAGACAG CAGCTGTGTG 
TCAGGGCAGA TCCTGGGCAA GGGCCTGGAG GACTTGTCCT ACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834744.1
CDS	346..3933
Translation	MEGPLEDGDESPESQGHATDWRFAVCSFRDAWDEGEPAPQMQAKNPHPPSPPAGAAQGEELQGSPLPGELQSPTGQMVADESGHGQKGTLRGSSVHMKEPASSGVKILLSPMSSHLSLAQGENDSQGGDLAGDSVSGRVMPVRLDPEVLDSDPVNHGGLLSETSSELLEADSHRSRLLKSTDYLLAQDIAWELLASGMAALPGTRDVEGRAVLLLCAHNSAWNHPKCNSHELVRLLLYLQSIPRPKVQTLGLTVLVDARICSPSSSIFWGLSQLQEAVPGSVHRVLLVGKLPEEVPAGLQIEHLSSHQSLLTHISTSELLTSLGGGLPYCHQAWLDFRMRLEALLQSCQVVCALLQGAIESMKAVPQPVESGEVGQLLQQARVLMKHVLDSPRLAWLQCQGALELTWLKQEVPEVTLSPDYRSAVDEVEELYGRVDGLLHQLTLQGNRRIQALELVQTLEAQEGELHQMEVWLQQVGWPALEEPREPSLDVLLQAQGPFRELDQIAQEQVRRGEKCLQPLVGCDAAELGPFGARFLTLQTQLADFSRALAQRRQRLADAERLLQFFKQALTWAEEGRQVLAELEQEHPGVVLQRLQLHWTKYPDLPPAHFRKMWALATGLGSEAIRHECRGAWAQCQDTWLALDQKLEAALKSPPTGSTASLCVSRVPAVPAVPPLRKAYSLDRNLGLSLREPAHQCHHEAIVPACHRPEAGGGAQSGSCPTVPPPGSCDPRSPNRLQLVLAEMVATEREYVRALDYTMENYFPELDRPDVPQGLRGQRAQLFGNLEKLWDFHRHFFLRELEACTQHPPRVAYAFLRHRVQFGMYALYSKNKPRSDALMTSYGHVFFRDKQQALGDHLDLASYLLKPIQRMSKYALLLQELARACGGPAQELSALRAAQSLVHFQLRHGNDLLAMDAIQGCDVNLKEQGQLVRQDEFTVRCGRHKSLRRIFLFEELLLFSKPRRGPTGIDMFTYKRSFKMADLGLTECCGDNNLRFEIWFRRRKARDTFVLQAASLATKQAWTADISRLLWRQAVHNKEVRMAEMVSMGVGNKAFRDIAPSEEAINDRTVNYILKCQVRSRASIAVAPLDSDSPYLGASGTLPGDPASCSVLGSLNLHLHRDPALLGFRWPLYSTSFPEEAALETDAELGSQPSLTPEDSEVSSQCPSASGSSGSDSSCVSGQILGKGLEDLSYV

Target ORF information:

RefSeq Version	XM_024978976.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024978976.1

ORF Insert Sequence:

ATGGAAGGGC CCCTGGAGGA TGGGGATGAG TCTCCAGAGT CCCAGGGCCA TGCCACCGAC 
TGGAGATTTG CTGTGTGCAG TTTCAGAGAT GCCTGGGATG AGGGGGAACC TGCTCCCCAG 
ATGCAGGCTA AGAACCCCCA TCCTCCAAGC CCACCAGCAG GGGCAGCCCA GGGGGAGGAG 
CTCCAGGGCA GCCCTCTGCC TGGTGAACTT CAGTCACCCA CAGGACAGAT GGTTGCAGAT 
GAGTCGGGGC ATGGCCAGAA GGGCACATTA AGAGGATCTT CTGTCCACAT GAAGGAACCA 
GCCTCCTCTG GAGTGAAGAT CCTCCTAAGT CCAATGTCTT CCCACCTTAG TCTGGCACAG 
GGTGAGAATG ACAGCCAAGG AGGAGACTTG GCAGGGGACT CAGTTTCAGG CAGGGTCATG 
CCAGTCCGCT TGGACCCTGA AGTGTTGGAC AGTGACCCTG TGAACCATGG AGGTCTTTTA 
TCTGAGACAT CGTCAGAGCT ACTGGAGGCA GACTCCCACA GATCCAGACT CTTGAAGTCC 
ACTGACTACC TCCTGGCCCA AGACATTGCC TGGGAGCTAC TTGCCAGTGG CATGGCTGCC 
CTGCCAGGGA CTCGAGATGT AGAAGGCCGG GCAGTGCTGC TTCTGTGTGC CCATAACTCA 
GCTTGGAATC ACCCCAAGTG CAACAGCCAT GAGCTCGTCC GCCTCCTGCT CTACTTGCAA 
AGCATCCCCA GGCCCAAAGT ACAGACCTTG GGACTGACCG TGCTAGTGGA CGCCCGGATT 
TGTTCCCCAA GCTCTTCCAT ATTCTGGGGG CTCAGCCAAC TGCAAGAAGC AGTCCCAGGA 
TCAGTGCACC GTGTGCTGCT GGTGGGAAAG CTGCCGGAGG AAGTGCCTGC AGGGCTTCAG 
ATAGAGCATC TATCCTCTCA CCAAAGCCTG CTGACCCACA TCTCCACCTC AGAGTTGCTC 
ACTTCACTAG GAGGAGGCCT GCCTTACTGT CACCAAGCTT GGCTGGACTT CCGGATGCGT 
CTGGAAGCCC TCCTGCAGAG CTGCCAGGTG GTTTGTGCCC TGCTTCAGGG AGCCATTGAG 
AGCATGAAGG CTGTGCCTCA GCCTGTGGAG TCTGGGGAAG TCGGTCAACT GCTGCAGCAG 
GCACGGGTCC TGATGAAGCA TGTGCTAGAC TCTCCACGGT TGGCATGGCT ACAATGCCAG 
GGAGCCTTGG AGCTGACATG GCTAAAGCAA GAGGTCCCAG AGGTGACCCT GAGCCCAGAC 
TACAGGTCAG CAGTGGACGA GGTTGAGGAG CTGTATGGCC GCGTGGATGG ATTGTTGCAC 
CAACTGACAC TGCAGGGTAA CCGCCGAATA CAAGCACTGG AGTTGGTCCA GACGCTGGAG 
GCCCAGGAGG GCGAGCTGCA CCAGATGGAA GTGTGGCTTC AGCAGGTGGG CTGGCCAGCA 
CTTGAGGAGC CCAGGGAGCC TTCACTAGAC GTGCTGCTTC AGGCCCAAGG CCCTTTTCGG 
GAGCTGGACC AGATTGCTCA GGAACAGGTC AGGCGAGGGG AGAAATGTCT GCAGCCACTG 
GTTGGCTGTG ATGCGGCCGA ACTGGGCCCC TTTGGGGCCC GCTTTCTGAC CCTGCAAACC 
CAGCTGGCCG ACTTCTCTAG GGCTTTGGCC CAGCGGCGGC AGCGACTGGC AGATGCTGAG 
AGACTCTTGC AGTTCTTCAA GCAGGCCTTG ACCTGGGCCG AGGAGGGGCG GCAAGTGTTG 
GCAGAGCTGG AACAGGAGCA CCCAGGGGTT GTGCTGCAGC GGCTGCAGCT GCATTGGACC 
AAGTACCCGG ACTTGCCTCC TGCCCACTTC CGAAAGATGT GGGCTTTAGC CACAGGGCTG 
GGATCTGAGG CCATTCGCCA TGAGTGCCGC GGGGCCTGGG CGCAGTGCCA GGACACCTGG 
CTGGCCCTGG ATCAGAAGTT AGAAGCTGCT CTGAAGTCAC CCCCAACGGG TAGCACAGCT 
AGCCTGTGTG TCAGCCGGGT CCCAGCGGTA CCGGCTGTCC CTCCCCTGAG GAAAGCCTAT 
AGCCTTGATC GGAATCTGGG ATTGAGTCTT AGAGAACCTG CCCACCAGTG CCACCATGAG 
GCCATTGTAC CTGCCTGCCA CAGACCAGAG GCTGGAGGTG GTGCCCAGTC AGGGTCATGC 
CCCACCGTGC CTCCTCCAGG GAGCTGTGAC CCCAGGAGCC CCAACAGACT TCAGCTGGTG 
CTGGCGGAGA TGGTGGCTAC GGAGCGGGAG TATGTCCGTG CCCTGGACTA CACCATGGAG 
AACTACTTCC CTGAGCTGGA TCGCCCTGAT GTGCCCCAGG GCCTCCGTGG CCAGCGTGCC 
CAACTCTTTG GCAACCTGGA GAAGCTGTGG GACTTCCACC GCCATTTTTT CTTGCGTGAG 
CTGGAGGCCT GTACCCAGCA CCCGCCCCGG GTGGCCTATG CCTTCCTGCG CCATAGGGTG 
CAGTTTGGGA TGTACGCACT CTACAGCAAG AATAAACCGC GCTCCGATGC CCTGATGACC 
AGCTATGGTC ACGTCTTCTT CAGGGACAAG CAGCAGGCAC TGGGGGACCA CTTGGACTTG 
GCCTCCTACC TGCTGAAGCC CATCCAGCGC ATGAGCAAGT ATGCGCTGCT GCTGCAGGAG 
CTGGCACGGG CCTGCGGGGG ACCCGCACAG GAGCTGAGTG CCCTGCGGGC TGCCCAGAGC 
CTCGTGCACT TCCAGCTGCG ACACGGCAAT GACTTGCTAG CCATGGATGC CATCCAGGGC 
TGTGACGTCA ACCTCAAGGA ACAGGGCCAG TTGGTCCGGC AGGATGAGTT CACAGTCCGC 
TGTGGGCGCC ACAAATCCCT GCGTCGCATT TTCCTCTTTG AGGAGCTGTT GCTCTTCAGC 
AAGCCTCGCC GAGGACCCAC AGGCATTGAC ATGTTTACCT ACAAGCGCTC CTTCAAGATG 
GCAGACCTTG GCCTCACTGA GTGCTGTGGG GACAACAACT TGCGCTTTGA GATCTGGTTT 
CGCCGTCGCA AGGCTAGGGA CACCTTTGTG CTACAAGCTG CCAGCTTGGC CACCAAGCAG 
GCCTGGACAG CTGACATCTC CCGCCTGCTC TGGAGGCAGG CCGTCCACAA CAAGGAGGTG 
CGCATGGCTG AGATGGTGTC TATGGGTGTG GGGAACAAGG CCTTCCGGGA CATCGCACCC 
AGCGAAGAAG CTATCAACGA CCGCACCGTC AACTATATCC TGAAGTGCCA AGTTCGCTCT 
CGGGCCTCCA TTGCTGTGGC TCCTCTTGAC TCTGACAGCC CCTACCTGGG GGCCTCAGGC 
ACCCTTCCTG GAGATCCTGC CTCTTGCTCT GTACTGGGGT CCCTCAACCT GCATCTGCAC 
AGAGACCCAG CTCTTCTGGG GTTCCGCTGG CCCCTGTATT CCACCAGTTT CCCAGAGGAA 
GCAGCACTGG AGACTGATGC CGAACTGGGC AGCCAGCCAT CTTTGACTCC TGAGGACTCA 
GAGGTTTCCT CCCAATGCCC ATCAGCCAGT GGCTCCAGTG GCTCAGACAG CAGCTGTGTG 
TCAGGGCAGA TCCTGGGCAA GGGCCTGGAG GACTTGTCCT ACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHG4 ( XM_015475823.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015331309.1 Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X2, mRNA.

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CloneID	OBa09337
Clone ID Related Accession (Same CDS sequence)	XM_015475823.1 , XM_005218850.2 , XM_015467369.1 , XM_005195262.2
Accession Version	XM_015475823.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	puratrophin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGGC CCCTGGAGGA TGGGGATGAG TCTCCAGAGT CCCAGGGCCA TGCCACCGAC 
TGGAGATTTG CTGTGTGCAG TTTCAGAGAT GCCTGGGATG AGGGGGAACC TGCTCCCCAG 
ATGCAGGCTA AGAACCCCCA TCCTCCAAGC CCACCAGCAG GGGCAGCCCA GGGGGAGGAG 
CTCCAGGGCA GCCCTCTGCC TGGTGAACTT CAGTCACCCA CAGGACAGAT GGTTGCAGAT 
GAGTCGGGGC ATGGCCAGAA GGGCACATTA AGAGGATCTT CTGTCCACAT GAAGGAACCA 
GCCTCCTCTG GAGTGAAGAT CCTCCTAAGT CCAATGTCTT CCCACCTTAG TCTGGCACAG 
GGTGAGAATG ACAGCCAAGG AGGAGACTTG GCAGGGGACT CAGTTTCAGG CAGGGTCATG 
CCAGTCCGCT TGGACCCTGA AGTGTTGGAC AGTGACCCTG TGAACCATGG AGGTCTTTTA 
TCTGAGACAT CGTCAGAGCT ACTGGAGGCA GACTCCCACA GATCCAGACT CTTGAAGTCC 
ACTGACTACC TCCTGGCCCA AGACATTGCC TGGGAGCTAC TTGCCAGTGG CATGGCTGCC 
CTGCCAGGGA CTCGAGATGT AGAAGGCCGG GCAGTGCTGC TTCTGTGTGC CCATAACTCA 
GCTTGGAATC ACCCCAAGTG CAACAGCCAT GAGCTCGTCC GCCTCCTGCT CTACTTGCAA 
AGCATCCCCA GGCCCAAAGT ACAGACCTTG GGACTGACCG TGCTAGTGGA CGCCCGGATT 
TGTTCCCCAA GCTCTTCCAT ATTCTGGGGG CTCAGCCAAC TGCAAGAAGC AGTCCCAGGA 
TCAGTGCACC GTGTGCTGCT GGTGGGAAAG CTGCCGGAGG AAGTGCCTGC AGGGCTTCAG 
ATAGAGCATC TATCCTCTCA CCAAAGCCTG CTGACCCACA TCTCCACCTC AGAGTTGCTC 
ACTTCACTAG GAGGAGGCCT GCCTTACTGT CACCAAGCTT GGCTGGACTT CCGGATGCGT 
CTGGAAGCCC TCCTGCAGAG CTGCCAGGTG GTTTGTGCCC TGCTTCAGGG AGCCATTGAG 
AGCATGAAGG CTGTGCCTCA GCCTGTGGAG TCTGGGGAAG TCGGTCAACT GCTGCAGCAG 
GCACGGGTCC TGATGAAGCA TGTGCTAGAC TCTCCACGGT TGGCATGGCT ACAATGCCAG 
GGAGCCTTGG AGCTGACATG GCTAAAGCAA GAGGTCCCAG AGGTGACCCT GAGCCCAGAC 
TACAGGTCAG CAGTGGACGA GGTTGAGGAG CTGTATGGCC GCGTGGATGG ATTGTTGCAC 
CAACTGACAC TGCAGGGTAA CCGCCGAATA CAAGCACTGG AGTTGGTCCA GACGCTGGAG 
GCCCAGGAGG GCGAGCTGCA CCAGATGGAA GTGTGGCTTC AGCAGGTGGG CTGGCCAGCA 
CTTGAGGAGC CCAGGGAGCC TTCACTAGAC GTGCTGCTTC AGGCCCAAGG CCCTTTTCGG 
GAGCTGGACC AGATTGCTCA GGAACAGGTC AGGCGAGGGG AGAAATGTCT GCAGCCACTG 
GTTGGCTGTG ATGCGGCCGA ACTGGGCCCC TTTGGGGCCC GCTTTCTGAC CCTGCAAACC 
CAGCTGGCCG ACTTCTCTAG GGCTTTGGCC CAGCGGCGGC AGCGACTGGC AGATGCTGAG 
AGACTCTTGC AGTTCTTCAA GCAGGCCTTG ACCTGGGCCG AGGAGGGGCG GCAAGTGTTG 
GCAGAGCTGG AACAGGAGCA CCCAGGGGTT GTGCTGCAGC GGCTGCAGCT GCATTGGACC 
AAGTACCCGG ACTTGCCTCC TGCCCACTTC CGAAAGATGT GGGCTTTAGC CACAGGGCTG 
GGATCTGAGG CCATTCGCCA TGAGTGCCGC GGGGCCTGGG CGCAGTGCCA GGACACCTGG 
CTGGCCCTGG ATCAGAAGTT AGAAGCTGCT CTGAAGTCAC CCCCAACGGG TAGCACAGCT 
AGCCTGTGTG TCAGCCGGGT CCCAGCGGTA CCGGCTGTCC CTCCCCTGAG GAAAGCCTAT 
AGCCTTGATC GGAATCTGGG ATTGAGTCTT AGAGAACCTG CCCACCAGTG CCACCATGAG 
GCCATTGTAC CTGCCTGCCA CAGACCAGAG GCTGGAGGTG GTGCCCAGTC AGGGTCATGC 
CCCACCGTGC CTCCTCCAGG GAGCTGTGAC CCCAGGAGCC CCAACAGACT TCAGCTGGTG 
CTGGCGGAGA TGGTGGCTAC GGAGCGGGAG TATGTCCGTG CCCTGGACTA CACCATGGAG 
AACTACTTCC CTGAGCTGGA TCGCCCTGAT GTGCCCCAGG GCCTCCGTGG CCAGCGTGCC 
CAACTCTTTG GCAACCTGGA GAAGCTGTGG GACTTCCACC GCCATTTTTT CTTGCGTGAG 
CTGGAGGCCT GTACCCAGCA CCCGCCCCGG GTGGCCTATG CCTTCCTGCG CCATAGGGTG 
CAGTTTGGGA TGTACGCACT CTACAGCAAG AATAAACCGC GCTCCGATGC CCTGATGACC 
AGCTATGGTC ACGTCTTCTT CAGGGACAAG CAGCAGGCAC TGGGGGACCA CTTGGACTTG 
GCCTCCTACC TGCTGAAGCC CATCCAGCGC ATGAGCAAGT ATGCGCTGCT GCTGCAGGAG 
CTGGCACGGG CCTGCGGGGG ACCCGCACAG GAGCTGAGTG CCCTGCGGGC TGCCCAGAGC 
CTCGTGCACT TCCAGCTGCG ACACGGCAAT GACTTGCTAG CCATGGATGC CATCCAGGGC 
TGTGACGTCA ACCTCAAGGA ACAGGGCCAG TTGGTCCGGC AGGATGAGTT CACAGTCCGC 
TGTGGGCGCC ACAAATCCCT GCGTCGCATT TTCCTCTTTG AGGAGCTGTT GCTCTTCAGC 
AAGCCTCGCC GAGGACCCAC AGGCATTGAC ATGTTTACCT ACAAGCGCTC CTTCAAGATG 
GCAGACCTTG GCCTCACTGA GTGCTGTGGG GACAACAACT TGCGCTTTGA GATCTGGTTT 
CGCCGTCGCA AGGCTAGGGA CACCTTTGTG CTACAAGCTG CCAGCTTGGC CACCAAGCAG 
GCCTGGACAG CTGACATCTC CCGCCTGCTC TGGAGGCAGG CCGTCCACAA CAAGGAGGTG 
CGCATGGCTG AGATGGTGTC TATGGGTGTG GGGAACAAGG CCTTCCGGGA CATCGCACCC 
AGCGAAGAAG CTATCAACGA CCGCACCGTC AACTATATCC TGAAGTGCCA AGCAGTTCGC 
TCTCGGGCCT CCATTGCTGT GGCTCCTCTT GACTCTGACA GCCCCTGCCT GGGGGCCTCA 
GGCACCCTTC CTGGAGATCC TGCCTCTTGC TCTGTACTGG GGTCCCTCAA CCTGCATCTG 
CACAGAGACC CAGCTCTTCT GGGGTTCCGC TGGCCCCTGT ATTCCACCAG TTTCCCAGAG 
GAAGCAGCAC TGGAGACTGA TGCCGAACTG GGCAGCCAGC CATCTTTGAC TCCTGAGGAC 
TCAGAGGTTT CCTCCCAATG CCCATCAGCC AGTGGCTCCA GTGGCTCAGA CAGCAGCTGT 
GTGTCAGGGC AGATCCTGGG CAAGGGCCTG GAGGACTTGT CCTACGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015331309.1
CDS	286..3876
Translation	MEGPLEDGDESPESQGHATDWRFAVCSFRDAWDEGEPAPQMQAKNPHPPSPPAGAAQGEELQGSPLPGELQSPTGQMVADESGHGQKGTLRGSSVHMKEPASSGVKILLSPMSSHLSLAQGENDSQGGDLAGDSVSGRVMPVRLDPEVLDSDPVNHGGLLSETSSELLEADSHRSRLLKSTDYLLAQDIAWELLASGMAALPGTRDVEGRAVLLLCAHNSAWNHPKCNSHELVRLLLYLQSIPRPKVQTLGLTVLVDARICSPSSSIFWGLSQLQEAVPGSVHRVLLVGKLPEEVPAGLQIEHLSSHQSLLTHISTSELLTSLGGGLPYCHQAWLDFRMRLEALLQSCQVVCALLQGAIESMKAVPQPVESGEVGQLLQQARVLMKHVLDSPRLAWLQCQGALELTWLKQEVPEVTLSPDYRSAVDEVEELYGRVDGLLHQLTLQGNRRIQALELVQTLEAQEGELHQMEVWLQQVGWPALEEPREPSLDVLLQAQGPFRELDQIAQEQVRRGEKCLQPLVGCDAAELGPFGARFLTLQTQLADFSRALAQRRQRLADAERLLQFFKQALTWAEEGRQVLAELEQEHPGVVLQRLQLHWTKYPDLPPAHFRKMWALATGLGSEAIRHECRGAWAQCQDTWLALDQKLEAALKSPPTGSTASLCVSRVPAVPAVPPLRKAYSLDRNLGLSLREPAHQCHHEAIVPACHRPEAGGGAQSGSCPTVPPPGSCDPRSPNRLQLVLAEMVATEREYVRALDYTMENYFPELDRPDVPQGLRGQRAQLFGNLEKLWDFHRHFFLRELEACTQHPPRVAYAFLRHRVQFGMYALYSKNKPRSDALMTSYGHVFFRDKQQALGDHLDLASYLLKPIQRMSKYALLLQELARACGGPAQELSALRAAQSLVHFQLRHGNDLLAMDAIQGCDVNLKEQGQLVRQDEFTVRCGRHKSLRRIFLFEELLLFSKPRRGPTGIDMFTYKRSFKMADLGLTECCGDNNLRFEIWFRRRKARDTFVLQAASLATKQAWTADISRLLWRQAVHNKEVRMAEMVSMGVGNKAFRDIAPSEEAINDRTVNYILKCQAVRSRASIAVAPLDSDSPCLGASGTLPGDPASCSVLGSLNLHLHRDPALLGFRWPLYSTSFPEEAALETDAELGSQPSLTPEDSEVSSQCPSASGSSGSDSSCVSGQILGKGLEDLSYV

Target ORF information:

RefSeq Version	XM_015475823.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475823.1

ORF Insert Sequence:

ATGGAAGGGC CCCTGGAGGA TGGGGATGAG TCTCCAGAGT CCCAGGGCCA TGCCACCGAC 
TGGAGATTTG CTGTGTGCAG TTTCAGAGAT GCCTGGGATG AGGGGGAACC TGCTCCCCAG 
ATGCAGGCTA AGAACCCCCA TCCTCCAAGC CCACCAGCAG GGGCAGCCCA GGGGGAGGAG 
CTCCAGGGCA GCCCTCTGCC TGGTGAACTT CAGTCACCCA CAGGACAGAT GGTTGCAGAT 
GAGTCGGGGC ATGGCCAGAA GGGCACATTA AGAGGATCTT CTGTCCACAT GAAGGAACCA 
GCCTCCTCTG GAGTGAAGAT CCTCCTAAGT CCAATGTCTT CCCACCTTAG TCTGGCACAG 
GGTGAGAATG ACAGCCAAGG AGGAGACTTG GCAGGGGACT CAGTTTCAGG CAGGGTCATG 
CCAGTCCGCT TGGACCCTGA AGTGTTGGAC AGTGACCCTG TGAACCATGG AGGTCTTTTA 
TCTGAGACAT CGTCAGAGCT ACTGGAGGCA GACTCCCACA GATCCAGACT CTTGAAGTCC 
ACTGACTACC TCCTGGCCCA AGACATTGCC TGGGAGCTAC TTGCCAGTGG CATGGCTGCC 
CTGCCAGGGA CTCGAGATGT AGAAGGCCGG GCAGTGCTGC TTCTGTGTGC CCATAACTCA 
GCTTGGAATC ACCCCAAGTG CAACAGCCAT GAGCTCGTCC GCCTCCTGCT CTACTTGCAA 
AGCATCCCCA GGCCCAAAGT ACAGACCTTG GGACTGACCG TGCTAGTGGA CGCCCGGATT 
TGTTCCCCAA GCTCTTCCAT ATTCTGGGGG CTCAGCCAAC TGCAAGAAGC AGTCCCAGGA 
TCAGTGCACC GTGTGCTGCT GGTGGGAAAG CTGCCGGAGG AAGTGCCTGC AGGGCTTCAG 
ATAGAGCATC TATCCTCTCA CCAAAGCCTG CTGACCCACA TCTCCACCTC AGAGTTGCTC 
ACTTCACTAG GAGGAGGCCT GCCTTACTGT CACCAAGCTT GGCTGGACTT CCGGATGCGT 
CTGGAAGCCC TCCTGCAGAG CTGCCAGGTG GTTTGTGCCC TGCTTCAGGG AGCCATTGAG 
AGCATGAAGG CTGTGCCTCA GCCTGTGGAG TCTGGGGAAG TCGGTCAACT GCTGCAGCAG 
GCACGGGTCC TGATGAAGCA TGTGCTAGAC TCTCCACGGT TGGCATGGCT ACAATGCCAG 
GGAGCCTTGG AGCTGACATG GCTAAAGCAA GAGGTCCCAG AGGTGACCCT GAGCCCAGAC 
TACAGGTCAG CAGTGGACGA GGTTGAGGAG CTGTATGGCC GCGTGGATGG ATTGTTGCAC 
CAACTGACAC TGCAGGGTAA CCGCCGAATA CAAGCACTGG AGTTGGTCCA GACGCTGGAG 
GCCCAGGAGG GCGAGCTGCA CCAGATGGAA GTGTGGCTTC AGCAGGTGGG CTGGCCAGCA 
CTTGAGGAGC CCAGGGAGCC TTCACTAGAC GTGCTGCTTC AGGCCCAAGG CCCTTTTCGG 
GAGCTGGACC AGATTGCTCA GGAACAGGTC AGGCGAGGGG AGAAATGTCT GCAGCCACTG 
GTTGGCTGTG ATGCGGCCGA ACTGGGCCCC TTTGGGGCCC GCTTTCTGAC CCTGCAAACC 
CAGCTGGCCG ACTTCTCTAG GGCTTTGGCC CAGCGGCGGC AGCGACTGGC AGATGCTGAG 
AGACTCTTGC AGTTCTTCAA GCAGGCCTTG ACCTGGGCCG AGGAGGGGCG GCAAGTGTTG 
GCAGAGCTGG AACAGGAGCA CCCAGGGGTT GTGCTGCAGC GGCTGCAGCT GCATTGGACC 
AAGTACCCGG ACTTGCCTCC TGCCCACTTC CGAAAGATGT GGGCTTTAGC CACAGGGCTG 
GGATCTGAGG CCATTCGCCA TGAGTGCCGC GGGGCCTGGG CGCAGTGCCA GGACACCTGG 
CTGGCCCTGG ATCAGAAGTT AGAAGCTGCT CTGAAGTCAC CCCCAACGGG TAGCACAGCT 
AGCCTGTGTG TCAGCCGGGT CCCAGCGGTA CCGGCTGTCC CTCCCCTGAG GAAAGCCTAT 
AGCCTTGATC GGAATCTGGG ATTGAGTCTT AGAGAACCTG CCCACCAGTG CCACCATGAG 
GCCATTGTAC CTGCCTGCCA CAGACCAGAG GCTGGAGGTG GTGCCCAGTC AGGGTCATGC 
CCCACCGTGC CTCCTCCAGG GAGCTGTGAC CCCAGGAGCC CCAACAGACT TCAGCTGGTG 
CTGGCGGAGA TGGTGGCTAC GGAGCGGGAG TATGTCCGTG CCCTGGACTA CACCATGGAG 
AACTACTTCC CTGAGCTGGA TCGCCCTGAT GTGCCCCAGG GCCTCCGTGG CCAGCGTGCC 
CAACTCTTTG GCAACCTGGA GAAGCTGTGG GACTTCCACC GCCATTTTTT CTTGCGTGAG 
CTGGAGGCCT GTACCCAGCA CCCGCCCCGG GTGGCCTATG CCTTCCTGCG CCATAGGGTG 
CAGTTTGGGA TGTACGCACT CTACAGCAAG AATAAACCGC GCTCCGATGC CCTGATGACC 
AGCTATGGTC ACGTCTTCTT CAGGGACAAG CAGCAGGCAC TGGGGGACCA CTTGGACTTG 
GCCTCCTACC TGCTGAAGCC CATCCAGCGC ATGAGCAAGT ATGCGCTGCT GCTGCAGGAG 
CTGGCACGGG CCTGCGGGGG ACCCGCACAG GAGCTGAGTG CCCTGCGGGC TGCCCAGAGC 
CTCGTGCACT TCCAGCTGCG ACACGGCAAT GACTTGCTAG CCATGGATGC CATCCAGGGC 
TGTGACGTCA ACCTCAAGGA ACAGGGCCAG TTGGTCCGGC AGGATGAGTT CACAGTCCGC 
TGTGGGCGCC ACAAATCCCT GCGTCGCATT TTCCTCTTTG AGGAGCTGTT GCTCTTCAGC 
AAGCCTCGCC GAGGACCCAC AGGCATTGAC ATGTTTACCT ACAAGCGCTC CTTCAAGATG 
GCAGACCTTG GCCTCACTGA GTGCTGTGGG GACAACAACT TGCGCTTTGA GATCTGGTTT 
CGCCGTCGCA AGGCTAGGGA CACCTTTGTG CTACAAGCTG CCAGCTTGGC CACCAAGCAG 
GCCTGGACAG CTGACATCTC CCGCCTGCTC TGGAGGCAGG CCGTCCACAA CAAGGAGGTG 
CGCATGGCTG AGATGGTGTC TATGGGTGTG GGGAACAAGG CCTTCCGGGA CATCGCACCC 
AGCGAAGAAG CTATCAACGA CCGCACCGTC AACTATATCC TGAAGTGCCA AGCAGTTCGC 
TCTCGGGCCT CCATTGCTGT GGCTCCTCTT GACTCTGACA GCCCCTGCCT GGGGGCCTCA 
GGCACCCTTC CTGGAGATCC TGCCTCTTGC TCTGTACTGG GGTCCCTCAA CCTGCATCTG 
CACAGAGACC CAGCTCTTCT GGGGTTCCGC TGGCCCCTGT ATTCCACCAG TTTCCCAGAG 
GAAGCAGCAC TGGAGACTGA TGCCGAACTG GGCAGCCAGC CATCTTTGAC TCCTGAGGAC 
TCAGAGGTTT CCTCCCAATG CCCATCAGCC AGTGGCTCCA GTGGCTCAGA CAGCAGCTGT 
GTGTCAGGGC AGATCCTGGG CAAGGGCCTG GAGGACTTGT CCTACGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHG4 ( XM_005218850.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218907.1 Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa09337
Clone ID Related Accession (Same CDS sequence)	XM_015475823.1 , XM_005218850.2 , XM_015467369.1 , XM_005195262.2
Accession Version	XM_005218850.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	puratrophin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005218850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGGC CCCTGGAGGA TGGGGATGAG TCTCCAGAGT CCCAGGGCCA TGCCACCGAC 
TGGAGATTTG CTGTGTGCAG TTTCAGAGAT GCCTGGGATG AGGGGGAACC TGCTCCCCAG 
ATGCAGGCTA AGAACCCCCA TCCTCCAAGC CCACCAGCAG GGGCAGCCCA GGGGGAGGAG 
CTCCAGGGCA GCCCTCTGCC TGGTGAACTT CAGTCACCCA CAGGACAGAT GGTTGCAGAT 
GAGTCGGGGC ATGGCCAGAA GGGCACATTA AGAGGATCTT CTGTCCACAT GAAGGAACCA 
GCCTCCTCTG GAGTGAAGAT CCTCCTAAGT CCAATGTCTT CCCACCTTAG TCTGGCACAG 
GGTGAGAATG ACAGCCAAGG AGGAGACTTG GCAGGGGACT CAGTTTCAGG CAGGGTCATG 
CCAGTCCGCT TGGACCCTGA AGTGTTGGAC AGTGACCCTG TGAACCATGG AGGTCTTTTA 
TCTGAGACAT CGTCAGAGCT ACTGGAGGCA GACTCCCACA GATCCAGACT CTTGAAGTCC 
ACTGACTACC TCCTGGCCCA AGACATTGCC TGGGAGCTAC TTGCCAGTGG CATGGCTGCC 
CTGCCAGGGA CTCGAGATGT AGAAGGCCGG GCAGTGCTGC TTCTGTGTGC CCATAACTCA 
GCTTGGAATC ACCCCAAGTG CAACAGCCAT GAGCTCGTCC GCCTCCTGCT CTACTTGCAA 
AGCATCCCCA GGCCCAAAGT ACAGACCTTG GGACTGACCG TGCTAGTGGA CGCCCGGATT 
TGTTCCCCAA GCTCTTCCAT ATTCTGGGGG CTCAGCCAAC TGCAAGAAGC AGTCCCAGGA 
TCAGTGCACC GTGTGCTGCT GGTGGGAAAG CTGCCGGAGG AAGTGCCTGC AGGGCTTCAG 
ATAGAGCATC TATCCTCTCA CCAAAGCCTG CTGACCCACA TCTCCACCTC AGAGTTGCTC 
ACTTCACTAG GAGGAGGCCT GCCTTACTGT CACCAAGCTT GGCTGGACTT CCGGATGCGT 
CTGGAAGCCC TCCTGCAGAG CTGCCAGGTG GTTTGTGCCC TGCTTCAGGG AGCCATTGAG 
AGCATGAAGG CTGTGCCTCA GCCTGTGGAG TCTGGGGAAG TCGGTCAACT GCTGCAGCAG 
GCACGGGTCC TGATGAAGCA TGTGCTAGAC TCTCCACGGT TGGCATGGCT ACAATGCCAG 
GGAGCCTTGG AGCTGACATG GCTAAAGCAA GAGGTCCCAG AGGTGACCCT GAGCCCAGAC 
TACAGGTCAG CAGTGGACGA GGTTGAGGAG CTGTATGGCC GCGTGGATGG ATTGTTGCAC 
CAACTGACAC TGCAGGGTAA CCGCCGAATA CAAGCACTGG AGTTGGTCCA GACGCTGGAG 
GCCCAGGAGG GCGAGCTGCA CCAGATGGAA GTGTGGCTTC AGCAGGTGGG CTGGCCAGCA 
CTTGAGGAGC CCAGGGAGCC TTCACTAGAC GTGCTGCTTC AGGCCCAAGG CCCTTTTCGG 
GAGCTGGACC AGATTGCTCA GGAACAGGTC AGGCGAGGGG AGAAATGTCT GCAGCCACTG 
GTTGGCTGTG ATGCGGCCGA ACTGGGCCCC TTTGGGGCCC GCTTTCTGAC CCTGCAAACC 
CAGCTGGCCG ACTTCTCTAG GGCTTTGGCC CAGCGGCGGC AGCGACTGGC AGATGCTGAG 
AGACTCTTGC AGTTCTTCAA GCAGGCCTTG ACCTGGGCCG AGGAGGGGCG GCAAGTGTTG 
GCAGAGCTGG AACAGGAGCA CCCAGGGGTT GTGCTGCAGC GGCTGCAGCT GCATTGGACC 
AAGTACCCGG ACTTGCCTCC TGCCCACTTC CGAAAGATGT GGGCTTTAGC CACAGGGCTG 
GGATCTGAGG CCATTCGCCA TGAGTGCCGC GGGGCCTGGG CGCAGTGCCA GGACACCTGG 
CTGGCCCTGG ATCAGAAGTT AGAAGCTGCT CTGAAGTCAC CCCCAACGGG TAGCACAGCT 
AGCCTGTGTG TCAGCCGGGT CCCAGCGGTA CCGGCTGTCC CTCCCCTGAG GAAAGCCTAT 
AGCCTTGATC GGAATCTGGG ATTGAGTCTT AGAGAACCTG CCCACCAGTG CCACCATGAG 
GCCATTGTAC CTGCCTGCCA CAGACCAGAG GCTGGAGGTG GTGCCCAGTC AGGGTCATGC 
CCCACCGTGC CTCCTCCAGG GAGCTGTGAC CCCAGGAGCC CCAACAGACT TCAGCTGGTG 
CTGGCGGAGA TGGTGGCTAC GGAGCGGGAG TATGTCCGTG CCCTGGACTA CACCATGGAG 
AACTACTTCC CTGAGCTGGA TCGCCCTGAT GTGCCCCAGG GCCTCCGTGG CCAGCGTGCC 
CAACTCTTTG GCAACCTGGA GAAGCTGTGG GACTTCCACC GCCATTTTTT CTTGCGTGAG 
CTGGAGGCCT GTACCCAGCA CCCGCCCCGG GTGGCCTATG CCTTCCTGCG CCATAGGGTG 
CAGTTTGGGA TGTACGCACT CTACAGCAAG AATAAACCGC GCTCCGATGC CCTGATGACC 
AGCTATGGTC ACGTCTTCTT CAGGGACAAG CAGCAGGCAC TGGGGGACCA CTTGGACTTG 
GCCTCCTACC TGCTGAAGCC CATCCAGCGC ATGAGCAAGT ATGCGCTGCT GCTGCAGGAG 
CTGGCACGGG CCTGCGGGGG ACCCGCACAG GAGCTGAGTG CCCTGCGGGC TGCCCAGAGC 
CTCGTGCACT TCCAGCTGCG ACACGGCAAT GACTTGCTAG CCATGGATGC CATCCAGGGC 
TGTGACGTCA ACCTCAAGGA ACAGGGCCAG TTGGTCCGGC AGGATGAGTT CACAGTCCGC 
TGTGGGCGCC ACAAATCCCT GCGTCGCATT TTCCTCTTTG AGGAGCTGTT GCTCTTCAGC 
AAGCCTCGCC GAGGACCCAC AGGCATTGAC ATGTTTACCT ACAAGCGCTC CTTCAAGATG 
GCAGACCTTG GCCTCACTGA GTGCTGTGGG GACAACAACT TGCGCTTTGA GATCTGGTTT 
CGCCGTCGCA AGGCTAGGGA CACCTTTGTG CTACAAGCTG CCAGCTTGGC CACCAAGCAG 
GCCTGGACAG CTGACATCTC CCGCCTGCTC TGGAGGCAGG CCGTCCACAA CAAGGAGGTG 
CGCATGGCTG AGATGGTGTC TATGGGTGTG GGGAACAAGG CCTTCCGGGA CATCGCACCC 
AGCGAAGAAG CTATCAACGA CCGCACCGTC AACTATATCC TGAAGTGCCA AGCAGTTCGC 
TCTCGGGCCT CCATTGCTGT GGCTCCTCTT GACTCTGACA GCCCCTGCCT GGGGGCCTCA 
GGCACCCTTC CTGGAGATCC TGCCTCTTGC TCTGTACTGG GGTCCCTCAA CCTGCATCTG 
CACAGAGACC CAGCTCTTCT GGGGTTCCGC TGGCCCCTGT ATTCCACCAG TTTCCCAGAG 
GAAGCAGCAC TGGAGACTGA TGCCGAACTG GGCAGCCAGC CATCTTTGAC TCCTGAGGAC 
TCAGAGGTTT CCTCCCAATG CCCATCAGCC AGTGGCTCCA GTGGCTCAGA CAGCAGCTGT 
GTGTCAGGGC AGATCCTGGG CAAGGGCCTG GAGGACTTGT CCTACGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218907.1
CDS	292..3882
Translation	MEGPLEDGDESPESQGHATDWRFAVCSFRDAWDEGEPAPQMQAKNPHPPSPPAGAAQGEELQGSPLPGELQSPTGQMVADESGHGQKGTLRGSSVHMKEPASSGVKILLSPMSSHLSLAQGENDSQGGDLAGDSVSGRVMPVRLDPEVLDSDPVNHGGLLSETSSELLEADSHRSRLLKSTDYLLAQDIAWELLASGMAALPGTRDVEGRAVLLLCAHNSAWNHPKCNSHELVRLLLYLQSIPRPKVQTLGLTVLVDARICSPSSSIFWGLSQLQEAVPGSVHRVLLVGKLPEEVPAGLQIEHLSSHQSLLTHISTSELLTSLGGGLPYCHQAWLDFRMRLEALLQSCQVVCALLQGAIESMKAVPQPVESGEVGQLLQQARVLMKHVLDSPRLAWLQCQGALELTWLKQEVPEVTLSPDYRSAVDEVEELYGRVDGLLHQLTLQGNRRIQALELVQTLEAQEGELHQMEVWLQQVGWPALEEPREPSLDVLLQAQGPFRELDQIAQEQVRRGEKCLQPLVGCDAAELGPFGARFLTLQTQLADFSRALAQRRQRLADAERLLQFFKQALTWAEEGRQVLAELEQEHPGVVLQRLQLHWTKYPDLPPAHFRKMWALATGLGSEAIRHECRGAWAQCQDTWLALDQKLEAALKSPPTGSTASLCVSRVPAVPAVPPLRKAYSLDRNLGLSLREPAHQCHHEAIVPACHRPEAGGGAQSGSCPTVPPPGSCDPRSPNRLQLVLAEMVATEREYVRALDYTMENYFPELDRPDVPQGLRGQRAQLFGNLEKLWDFHRHFFLRELEACTQHPPRVAYAFLRHRVQFGMYALYSKNKPRSDALMTSYGHVFFRDKQQALGDHLDLASYLLKPIQRMSKYALLLQELARACGGPAQELSALRAAQSLVHFQLRHGNDLLAMDAIQGCDVNLKEQGQLVRQDEFTVRCGRHKSLRRIFLFEELLLFSKPRRGPTGIDMFTYKRSFKMADLGLTECCGDNNLRFEIWFRRRKARDTFVLQAASLATKQAWTADISRLLWRQAVHNKEVRMAEMVSMGVGNKAFRDIAPSEEAINDRTVNYILKCQAVRSRASIAVAPLDSDSPCLGASGTLPGDPASCSVLGSLNLHLHRDPALLGFRWPLYSTSFPEEAALETDAELGSQPSLTPEDSEVSSQCPSASGSSGSDSSCVSGQILGKGLEDLSYV

Target ORF information:

RefSeq Version	XM_005218850.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218850.2

ORF Insert Sequence:

ATGGAAGGGC CCCTGGAGGA TGGGGATGAG TCTCCAGAGT CCCAGGGCCA TGCCACCGAC 
TGGAGATTTG CTGTGTGCAG TTTCAGAGAT GCCTGGGATG AGGGGGAACC TGCTCCCCAG 
ATGCAGGCTA AGAACCCCCA TCCTCCAAGC CCACCAGCAG GGGCAGCCCA GGGGGAGGAG 
CTCCAGGGCA GCCCTCTGCC TGGTGAACTT CAGTCACCCA CAGGACAGAT GGTTGCAGAT 
GAGTCGGGGC ATGGCCAGAA GGGCACATTA AGAGGATCTT CTGTCCACAT GAAGGAACCA 
GCCTCCTCTG GAGTGAAGAT CCTCCTAAGT CCAATGTCTT CCCACCTTAG TCTGGCACAG 
GGTGAGAATG ACAGCCAAGG AGGAGACTTG GCAGGGGACT CAGTTTCAGG CAGGGTCATG 
CCAGTCCGCT TGGACCCTGA AGTGTTGGAC AGTGACCCTG TGAACCATGG AGGTCTTTTA 
TCTGAGACAT CGTCAGAGCT ACTGGAGGCA GACTCCCACA GATCCAGACT CTTGAAGTCC 
ACTGACTACC TCCTGGCCCA AGACATTGCC TGGGAGCTAC TTGCCAGTGG CATGGCTGCC 
CTGCCAGGGA CTCGAGATGT AGAAGGCCGG GCAGTGCTGC TTCTGTGTGC CCATAACTCA 
GCTTGGAATC ACCCCAAGTG CAACAGCCAT GAGCTCGTCC GCCTCCTGCT CTACTTGCAA 
AGCATCCCCA GGCCCAAAGT ACAGACCTTG GGACTGACCG TGCTAGTGGA CGCCCGGATT 
TGTTCCCCAA GCTCTTCCAT ATTCTGGGGG CTCAGCCAAC TGCAAGAAGC AGTCCCAGGA 
TCAGTGCACC GTGTGCTGCT GGTGGGAAAG CTGCCGGAGG AAGTGCCTGC AGGGCTTCAG 
ATAGAGCATC TATCCTCTCA CCAAAGCCTG CTGACCCACA TCTCCACCTC AGAGTTGCTC 
ACTTCACTAG GAGGAGGCCT GCCTTACTGT CACCAAGCTT GGCTGGACTT CCGGATGCGT 
CTGGAAGCCC TCCTGCAGAG CTGCCAGGTG GTTTGTGCCC TGCTTCAGGG AGCCATTGAG 
AGCATGAAGG CTGTGCCTCA GCCTGTGGAG TCTGGGGAAG TCGGTCAACT GCTGCAGCAG 
GCACGGGTCC TGATGAAGCA TGTGCTAGAC TCTCCACGGT TGGCATGGCT ACAATGCCAG 
GGAGCCTTGG AGCTGACATG GCTAAAGCAA GAGGTCCCAG AGGTGACCCT GAGCCCAGAC 
TACAGGTCAG CAGTGGACGA GGTTGAGGAG CTGTATGGCC GCGTGGATGG ATTGTTGCAC 
CAACTGACAC TGCAGGGTAA CCGCCGAATA CAAGCACTGG AGTTGGTCCA GACGCTGGAG 
GCCCAGGAGG GCGAGCTGCA CCAGATGGAA GTGTGGCTTC AGCAGGTGGG CTGGCCAGCA 
CTTGAGGAGC CCAGGGAGCC TTCACTAGAC GTGCTGCTTC AGGCCCAAGG CCCTTTTCGG 
GAGCTGGACC AGATTGCTCA GGAACAGGTC AGGCGAGGGG AGAAATGTCT GCAGCCACTG 
GTTGGCTGTG ATGCGGCCGA ACTGGGCCCC TTTGGGGCCC GCTTTCTGAC CCTGCAAACC 
CAGCTGGCCG ACTTCTCTAG GGCTTTGGCC CAGCGGCGGC AGCGACTGGC AGATGCTGAG 
AGACTCTTGC AGTTCTTCAA GCAGGCCTTG ACCTGGGCCG AGGAGGGGCG GCAAGTGTTG 
GCAGAGCTGG AACAGGAGCA CCCAGGGGTT GTGCTGCAGC GGCTGCAGCT GCATTGGACC 
AAGTACCCGG ACTTGCCTCC TGCCCACTTC CGAAAGATGT GGGCTTTAGC CACAGGGCTG 
GGATCTGAGG CCATTCGCCA TGAGTGCCGC GGGGCCTGGG CGCAGTGCCA GGACACCTGG 
CTGGCCCTGG ATCAGAAGTT AGAAGCTGCT CTGAAGTCAC CCCCAACGGG TAGCACAGCT 
AGCCTGTGTG TCAGCCGGGT CCCAGCGGTA CCGGCTGTCC CTCCCCTGAG GAAAGCCTAT 
AGCCTTGATC GGAATCTGGG ATTGAGTCTT AGAGAACCTG CCCACCAGTG CCACCATGAG 
GCCATTGTAC CTGCCTGCCA CAGACCAGAG GCTGGAGGTG GTGCCCAGTC AGGGTCATGC 
CCCACCGTGC CTCCTCCAGG GAGCTGTGAC CCCAGGAGCC CCAACAGACT TCAGCTGGTG 
CTGGCGGAGA TGGTGGCTAC GGAGCGGGAG TATGTCCGTG CCCTGGACTA CACCATGGAG 
AACTACTTCC CTGAGCTGGA TCGCCCTGAT GTGCCCCAGG GCCTCCGTGG CCAGCGTGCC 
CAACTCTTTG GCAACCTGGA GAAGCTGTGG GACTTCCACC GCCATTTTTT CTTGCGTGAG 
CTGGAGGCCT GTACCCAGCA CCCGCCCCGG GTGGCCTATG CCTTCCTGCG CCATAGGGTG 
CAGTTTGGGA TGTACGCACT CTACAGCAAG AATAAACCGC GCTCCGATGC CCTGATGACC 
AGCTATGGTC ACGTCTTCTT CAGGGACAAG CAGCAGGCAC TGGGGGACCA CTTGGACTTG 
GCCTCCTACC TGCTGAAGCC CATCCAGCGC ATGAGCAAGT ATGCGCTGCT GCTGCAGGAG 
CTGGCACGGG CCTGCGGGGG ACCCGCACAG GAGCTGAGTG CCCTGCGGGC TGCCCAGAGC 
CTCGTGCACT TCCAGCTGCG ACACGGCAAT GACTTGCTAG CCATGGATGC CATCCAGGGC 
TGTGACGTCA ACCTCAAGGA ACAGGGCCAG TTGGTCCGGC AGGATGAGTT CACAGTCCGC 
TGTGGGCGCC ACAAATCCCT GCGTCGCATT TTCCTCTTTG AGGAGCTGTT GCTCTTCAGC 
AAGCCTCGCC GAGGACCCAC AGGCATTGAC ATGTTTACCT ACAAGCGCTC CTTCAAGATG 
GCAGACCTTG GCCTCACTGA GTGCTGTGGG GACAACAACT TGCGCTTTGA GATCTGGTTT 
CGCCGTCGCA AGGCTAGGGA CACCTTTGTG CTACAAGCTG CCAGCTTGGC CACCAAGCAG 
GCCTGGACAG CTGACATCTC CCGCCTGCTC TGGAGGCAGG CCGTCCACAA CAAGGAGGTG 
CGCATGGCTG AGATGGTGTC TATGGGTGTG GGGAACAAGG CCTTCCGGGA CATCGCACCC 
AGCGAAGAAG CTATCAACGA CCGCACCGTC AACTATATCC TGAAGTGCCA AGCAGTTCGC 
TCTCGGGCCT CCATTGCTGT GGCTCCTCTT GACTCTGACA GCCCCTGCCT GGGGGCCTCA 
GGCACCCTTC CTGGAGATCC TGCCTCTTGC TCTGTACTGG GGTCCCTCAA CCTGCATCTG 
CACAGAGACC CAGCTCTTCT GGGGTTCCGC TGGCCCCTGT ATTCCACCAG TTTCCCAGAG 
GAAGCAGCAC TGGAGACTGA TGCCGAACTG GGCAGCCAGC CATCTTTGAC TCCTGAGGAC 
TCAGAGGTTT CCTCCCAATG CCCATCAGCC AGTGGCTCCA GTGGCTCAGA CAGCAGCTGT 
GTGTCAGGGC AGATCCTGGG CAAGGGCCTG GAGGACTTGT CCTACGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHG4 ( XM_015467369.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322855.1 Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa09337
Clone ID Related Accession (Same CDS sequence)	XM_015475823.1 , XM_005218850.2 , XM_015467369.1 , XM_005195262.2
Accession Version	XM_015467369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	puratrophin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGGC CCCTGGAGGA TGGGGATGAG TCTCCAGAGT CCCAGGGCCA TGCCACCGAC 
TGGAGATTTG CTGTGTGCAG TTTCAGAGAT GCCTGGGATG AGGGGGAACC TGCTCCCCAG 
ATGCAGGCTA AGAACCCCCA TCCTCCAAGC CCACCAGCAG GGGCAGCCCA GGGGGAGGAG 
CTCCAGGGCA GCCCTCTGCC TGGTGAACTT CAGTCACCCA CAGGACAGAT GGTTGCAGAT 
GAGTCGGGGC ATGGCCAGAA GGGCACATTA AGAGGATCTT CTGTCCACAT GAAGGAACCA 
GCCTCCTCTG GAGTGAAGAT CCTCCTAAGT CCAATGTCTT CCCACCTTAG TCTGGCACAG 
GGTGAGAATG ACAGCCAAGG AGGAGACTTG GCAGGGGACT CAGTTTCAGG CAGGGTCATG 
CCAGTCCGCT TGGACCCTGA AGTGTTGGAC AGTGACCCTG TGAACCATGG AGGTCTTTTA 
TCTGAGACAT CGTCAGAGCT ACTGGAGGCA GACTCCCACA GATCCAGACT CTTGAAGTCC 
ACTGACTACC TCCTGGCCCA AGACATTGCC TGGGAGCTAC TTGCCAGTGG CATGGCTGCC 
CTGCCAGGGA CTCGAGATGT AGAAGGCCGG GCAGTGCTGC TTCTGTGTGC CCATAACTCA 
GCTTGGAATC ACCCCAAGTG CAACAGCCAT GAGCTCGTCC GCCTCCTGCT CTACTTGCAA 
AGCATCCCCA GGCCCAAAGT ACAGACCTTG GGACTGACCG TGCTAGTGGA CGCCCGGATT 
TGTTCCCCAA GCTCTTCCAT ATTCTGGGGG CTCAGCCAAC TGCAAGAAGC AGTCCCAGGA 
TCAGTGCACC GTGTGCTGCT GGTGGGAAAG CTGCCGGAGG AAGTGCCTGC AGGGCTTCAG 
ATAGAGCATC TATCCTCTCA CCAAAGCCTG CTGACCCACA TCTCCACCTC AGAGTTGCTC 
ACTTCACTAG GAGGAGGCCT GCCTTACTGT CACCAAGCTT GGCTGGACTT CCGGATGCGT 
CTGGAAGCCC TCCTGCAGAG CTGCCAGGTG GTTTGTGCCC TGCTTCAGGG AGCCATTGAG 
AGCATGAAGG CTGTGCCTCA GCCTGTGGAG TCTGGGGAAG TCGGTCAACT GCTGCAGCAG 
GCACGGGTCC TGATGAAGCA TGTGCTAGAC TCTCCACGGT TGGCATGGCT ACAATGCCAG 
GGAGCCTTGG AGCTGACATG GCTAAAGCAA GAGGTCCCAG AGGTGACCCT GAGCCCAGAC 
TACAGGTCAG CAGTGGACGA GGTTGAGGAG CTGTATGGCC GCGTGGATGG ATTGTTGCAC 
CAACTGACAC TGCAGGGTAA CCGCCGAATA CAAGCACTGG AGTTGGTCCA GACGCTGGAG 
GCCCAGGAGG GCGAGCTGCA CCAGATGGAA GTGTGGCTTC AGCAGGTGGG CTGGCCAGCA 
CTTGAGGAGC CCAGGGAGCC TTCACTAGAC GTGCTGCTTC AGGCCCAAGG CCCTTTTCGG 
GAGCTGGACC AGATTGCTCA GGAACAGGTC AGGCGAGGGG AGAAATGTCT GCAGCCACTG 
GTTGGCTGTG ATGCGGCCGA ACTGGGCCCC TTTGGGGCCC GCTTTCTGAC CCTGCAAACC 
CAGCTGGCCG ACTTCTCTAG GGCTTTGGCC CAGCGGCGGC AGCGACTGGC AGATGCTGAG 
AGACTCTTGC AGTTCTTCAA GCAGGCCTTG ACCTGGGCCG AGGAGGGGCG GCAAGTGTTG 
GCAGAGCTGG AACAGGAGCA CCCAGGGGTT GTGCTGCAGC GGCTGCAGCT GCATTGGACC 
AAGTACCCGG ACTTGCCTCC TGCCCACTTC CGAAAGATGT GGGCTTTAGC CACAGGGCTG 
GGATCTGAGG CCATTCGCCA TGAGTGCCGC GGGGCCTGGG CGCAGTGCCA GGACACCTGG 
CTGGCCCTGG ATCAGAAGTT AGAAGCTGCT CTGAAGTCAC CCCCAACGGG TAGCACAGCT 
AGCCTGTGTG TCAGCCGGGT CCCAGCGGTA CCGGCTGTCC CTCCCCTGAG GAAAGCCTAT 
AGCCTTGATC GGAATCTGGG ATTGAGTCTT AGAGAACCTG CCCACCAGTG CCACCATGAG 
GCCATTGTAC CTGCCTGCCA CAGACCAGAG GCTGGAGGTG GTGCCCAGTC AGGGTCATGC 
CCCACCGTGC CTCCTCCAGG GAGCTGTGAC CCCAGGAGCC CCAACAGACT TCAGCTGGTG 
CTGGCGGAGA TGGTGGCTAC GGAGCGGGAG TATGTCCGTG CCCTGGACTA CACCATGGAG 
AACTACTTCC CTGAGCTGGA TCGCCCTGAT GTGCCCCAGG GCCTCCGTGG CCAGCGTGCC 
CAACTCTTTG GCAACCTGGA GAAGCTGTGG GACTTCCACC GCCATTTTTT CTTGCGTGAG 
CTGGAGGCCT GTACCCAGCA CCCGCCCCGG GTGGCCTATG CCTTCCTGCG CCATAGGGTG 
CAGTTTGGGA TGTACGCACT CTACAGCAAG AATAAACCGC GCTCCGATGC CCTGATGACC 
AGCTATGGTC ACGTCTTCTT CAGGGACAAG CAGCAGGCAC TGGGGGACCA CTTGGACTTG 
GCCTCCTACC TGCTGAAGCC CATCCAGCGC ATGAGCAAGT ATGCGCTGCT GCTGCAGGAG 
CTGGCACGGG CCTGCGGGGG ACCCGCACAG GAGCTGAGTG CCCTGCGGGC TGCCCAGAGC 
CTCGTGCACT TCCAGCTGCG ACACGGCAAT GACTTGCTAG CCATGGATGC CATCCAGGGC 
TGTGACGTCA ACCTCAAGGA ACAGGGCCAG TTGGTCCGGC AGGATGAGTT CACAGTCCGC 
TGTGGGCGCC ACAAATCCCT GCGTCGCATT TTCCTCTTTG AGGAGCTGTT GCTCTTCAGC 
AAGCCTCGCC GAGGACCCAC AGGCATTGAC ATGTTTACCT ACAAGCGCTC CTTCAAGATG 
GCAGACCTTG GCCTCACTGA GTGCTGTGGG GACAACAACT TGCGCTTTGA GATCTGGTTT 
CGCCGTCGCA AGGCTAGGGA CACCTTTGTG CTACAAGCTG CCAGCTTGGC CACCAAGCAG 
GCCTGGACAG CTGACATCTC CCGCCTGCTC TGGAGGCAGG CCGTCCACAA CAAGGAGGTG 
CGCATGGCTG AGATGGTGTC TATGGGTGTG GGGAACAAGG CCTTCCGGGA CATCGCACCC 
AGCGAAGAAG CTATCAACGA CCGCACCGTC AACTATATCC TGAAGTGCCA AGCAGTTCGC 
TCTCGGGCCT CCATTGCTGT GGCTCCTCTT GACTCTGACA GCCCCTGCCT GGGGGCCTCA 
GGCACCCTTC CTGGAGATCC TGCCTCTTGC TCTGTACTGG GGTCCCTCAA CCTGCATCTG 
CACAGAGACC CAGCTCTTCT GGGGTTCCGC TGGCCCCTGT ATTCCACCAG TTTCCCAGAG 
GAAGCAGCAC TGGAGACTGA TGCCGAACTG GGCAGCCAGC CATCTTTGAC TCCTGAGGAC 
TCAGAGGTTT CCTCCCAATG CCCATCAGCC AGTGGCTCCA GTGGCTCAGA CAGCAGCTGT 
GTGTCAGGGC AGATCCTGGG CAAGGGCCTG GAGGACTTGT CCTACGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322855.1
CDS	286..3876
Translation	MEGPLEDGDESPESQGHATDWRFAVCSFRDAWDEGEPAPQMQAKNPHPPSPPAGAAQGEELQGSPLPGELQSPTGQMVADESGHGQKGTLRGSSVHMKEPASSGVKILLSPMSSHLSLAQGENDSQGGDLAGDSVSGRVMPVRLDPEVLDSDPVNHGGLLSETSSELLEADSHRSRLLKSTDYLLAQDIAWELLASGMAALPGTRDVEGRAVLLLCAHNSAWNHPKCNSHELVRLLLYLQSIPRPKVQTLGLTVLVDARICSPSSSIFWGLSQLQEAVPGSVHRVLLVGKLPEEVPAGLQIEHLSSHQSLLTHISTSELLTSLGGGLPYCHQAWLDFRMRLEALLQSCQVVCALLQGAIESMKAVPQPVESGEVGQLLQQARVLMKHVLDSPRLAWLQCQGALELTWLKQEVPEVTLSPDYRSAVDEVEELYGRVDGLLHQLTLQGNRRIQALELVQTLEAQEGELHQMEVWLQQVGWPALEEPREPSLDVLLQAQGPFRELDQIAQEQVRRGEKCLQPLVGCDAAELGPFGARFLTLQTQLADFSRALAQRRQRLADAERLLQFFKQALTWAEEGRQVLAELEQEHPGVVLQRLQLHWTKYPDLPPAHFRKMWALATGLGSEAIRHECRGAWAQCQDTWLALDQKLEAALKSPPTGSTASLCVSRVPAVPAVPPLRKAYSLDRNLGLSLREPAHQCHHEAIVPACHRPEAGGGAQSGSCPTVPPPGSCDPRSPNRLQLVLAEMVATEREYVRALDYTMENYFPELDRPDVPQGLRGQRAQLFGNLEKLWDFHRHFFLRELEACTQHPPRVAYAFLRHRVQFGMYALYSKNKPRSDALMTSYGHVFFRDKQQALGDHLDLASYLLKPIQRMSKYALLLQELARACGGPAQELSALRAAQSLVHFQLRHGNDLLAMDAIQGCDVNLKEQGQLVRQDEFTVRCGRHKSLRRIFLFEELLLFSKPRRGPTGIDMFTYKRSFKMADLGLTECCGDNNLRFEIWFRRRKARDTFVLQAASLATKQAWTADISRLLWRQAVHNKEVRMAEMVSMGVGNKAFRDIAPSEEAINDRTVNYILKCQAVRSRASIAVAPLDSDSPCLGASGTLPGDPASCSVLGSLNLHLHRDPALLGFRWPLYSTSFPEEAALETDAELGSQPSLTPEDSEVSSQCPSASGSSGSDSSCVSGQILGKGLEDLSYV

Target ORF information:

RefSeq Version	XM_015467369.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015467369.1

ORF Insert Sequence:

ATGGAAGGGC CCCTGGAGGA TGGGGATGAG TCTCCAGAGT CCCAGGGCCA TGCCACCGAC 
TGGAGATTTG CTGTGTGCAG TTTCAGAGAT GCCTGGGATG AGGGGGAACC TGCTCCCCAG 
ATGCAGGCTA AGAACCCCCA TCCTCCAAGC CCACCAGCAG GGGCAGCCCA GGGGGAGGAG 
CTCCAGGGCA GCCCTCTGCC TGGTGAACTT CAGTCACCCA CAGGACAGAT GGTTGCAGAT 
GAGTCGGGGC ATGGCCAGAA GGGCACATTA AGAGGATCTT CTGTCCACAT GAAGGAACCA 
GCCTCCTCTG GAGTGAAGAT CCTCCTAAGT CCAATGTCTT CCCACCTTAG TCTGGCACAG 
GGTGAGAATG ACAGCCAAGG AGGAGACTTG GCAGGGGACT CAGTTTCAGG CAGGGTCATG 
CCAGTCCGCT TGGACCCTGA AGTGTTGGAC AGTGACCCTG TGAACCATGG AGGTCTTTTA 
TCTGAGACAT CGTCAGAGCT ACTGGAGGCA GACTCCCACA GATCCAGACT CTTGAAGTCC 
ACTGACTACC TCCTGGCCCA AGACATTGCC TGGGAGCTAC TTGCCAGTGG CATGGCTGCC 
CTGCCAGGGA CTCGAGATGT AGAAGGCCGG GCAGTGCTGC TTCTGTGTGC CCATAACTCA 
GCTTGGAATC ACCCCAAGTG CAACAGCCAT GAGCTCGTCC GCCTCCTGCT CTACTTGCAA 
AGCATCCCCA GGCCCAAAGT ACAGACCTTG GGACTGACCG TGCTAGTGGA CGCCCGGATT 
TGTTCCCCAA GCTCTTCCAT ATTCTGGGGG CTCAGCCAAC TGCAAGAAGC AGTCCCAGGA 
TCAGTGCACC GTGTGCTGCT GGTGGGAAAG CTGCCGGAGG AAGTGCCTGC AGGGCTTCAG 
ATAGAGCATC TATCCTCTCA CCAAAGCCTG CTGACCCACA TCTCCACCTC AGAGTTGCTC 
ACTTCACTAG GAGGAGGCCT GCCTTACTGT CACCAAGCTT GGCTGGACTT CCGGATGCGT 
CTGGAAGCCC TCCTGCAGAG CTGCCAGGTG GTTTGTGCCC TGCTTCAGGG AGCCATTGAG 
AGCATGAAGG CTGTGCCTCA GCCTGTGGAG TCTGGGGAAG TCGGTCAACT GCTGCAGCAG 
GCACGGGTCC TGATGAAGCA TGTGCTAGAC TCTCCACGGT TGGCATGGCT ACAATGCCAG 
GGAGCCTTGG AGCTGACATG GCTAAAGCAA GAGGTCCCAG AGGTGACCCT GAGCCCAGAC 
TACAGGTCAG CAGTGGACGA GGTTGAGGAG CTGTATGGCC GCGTGGATGG ATTGTTGCAC 
CAACTGACAC TGCAGGGTAA CCGCCGAATA CAAGCACTGG AGTTGGTCCA GACGCTGGAG 
GCCCAGGAGG GCGAGCTGCA CCAGATGGAA GTGTGGCTTC AGCAGGTGGG CTGGCCAGCA 
CTTGAGGAGC CCAGGGAGCC TTCACTAGAC GTGCTGCTTC AGGCCCAAGG CCCTTTTCGG 
GAGCTGGACC AGATTGCTCA GGAACAGGTC AGGCGAGGGG AGAAATGTCT GCAGCCACTG 
GTTGGCTGTG ATGCGGCCGA ACTGGGCCCC TTTGGGGCCC GCTTTCTGAC CCTGCAAACC 
CAGCTGGCCG ACTTCTCTAG GGCTTTGGCC CAGCGGCGGC AGCGACTGGC AGATGCTGAG 
AGACTCTTGC AGTTCTTCAA GCAGGCCTTG ACCTGGGCCG AGGAGGGGCG GCAAGTGTTG 
GCAGAGCTGG AACAGGAGCA CCCAGGGGTT GTGCTGCAGC GGCTGCAGCT GCATTGGACC 
AAGTACCCGG ACTTGCCTCC TGCCCACTTC CGAAAGATGT GGGCTTTAGC CACAGGGCTG 
GGATCTGAGG CCATTCGCCA TGAGTGCCGC GGGGCCTGGG CGCAGTGCCA GGACACCTGG 
CTGGCCCTGG ATCAGAAGTT AGAAGCTGCT CTGAAGTCAC CCCCAACGGG TAGCACAGCT 
AGCCTGTGTG TCAGCCGGGT CCCAGCGGTA CCGGCTGTCC CTCCCCTGAG GAAAGCCTAT 
AGCCTTGATC GGAATCTGGG ATTGAGTCTT AGAGAACCTG CCCACCAGTG CCACCATGAG 
GCCATTGTAC CTGCCTGCCA CAGACCAGAG GCTGGAGGTG GTGCCCAGTC AGGGTCATGC 
CCCACCGTGC CTCCTCCAGG GAGCTGTGAC CCCAGGAGCC CCAACAGACT TCAGCTGGTG 
CTGGCGGAGA TGGTGGCTAC GGAGCGGGAG TATGTCCGTG CCCTGGACTA CACCATGGAG 
AACTACTTCC CTGAGCTGGA TCGCCCTGAT GTGCCCCAGG GCCTCCGTGG CCAGCGTGCC 
CAACTCTTTG GCAACCTGGA GAAGCTGTGG GACTTCCACC GCCATTTTTT CTTGCGTGAG 
CTGGAGGCCT GTACCCAGCA CCCGCCCCGG GTGGCCTATG CCTTCCTGCG CCATAGGGTG 
CAGTTTGGGA TGTACGCACT CTACAGCAAG AATAAACCGC GCTCCGATGC CCTGATGACC 
AGCTATGGTC ACGTCTTCTT CAGGGACAAG CAGCAGGCAC TGGGGGACCA CTTGGACTTG 
GCCTCCTACC TGCTGAAGCC CATCCAGCGC ATGAGCAAGT ATGCGCTGCT GCTGCAGGAG 
CTGGCACGGG CCTGCGGGGG ACCCGCACAG GAGCTGAGTG CCCTGCGGGC TGCCCAGAGC 
CTCGTGCACT TCCAGCTGCG ACACGGCAAT GACTTGCTAG CCATGGATGC CATCCAGGGC 
TGTGACGTCA ACCTCAAGGA ACAGGGCCAG TTGGTCCGGC AGGATGAGTT CACAGTCCGC 
TGTGGGCGCC ACAAATCCCT GCGTCGCATT TTCCTCTTTG AGGAGCTGTT GCTCTTCAGC 
AAGCCTCGCC GAGGACCCAC AGGCATTGAC ATGTTTACCT ACAAGCGCTC CTTCAAGATG 
GCAGACCTTG GCCTCACTGA GTGCTGTGGG GACAACAACT TGCGCTTTGA GATCTGGTTT 
CGCCGTCGCA AGGCTAGGGA CACCTTTGTG CTACAAGCTG CCAGCTTGGC CACCAAGCAG 
GCCTGGACAG CTGACATCTC CCGCCTGCTC TGGAGGCAGG CCGTCCACAA CAAGGAGGTG 
CGCATGGCTG AGATGGTGTC TATGGGTGTG GGGAACAAGG CCTTCCGGGA CATCGCACCC 
AGCGAAGAAG CTATCAACGA CCGCACCGTC AACTATATCC TGAAGTGCCA AGCAGTTCGC 
TCTCGGGCCT CCATTGCTGT GGCTCCTCTT GACTCTGACA GCCCCTGCCT GGGGGCCTCA 
GGCACCCTTC CTGGAGATCC TGCCTCTTGC TCTGTACTGG GGTCCCTCAA CCTGCATCTG 
CACAGAGACC CAGCTCTTCT GGGGTTCCGC TGGCCCCTGT ATTCCACCAG TTTCCCAGAG 
GAAGCAGCAC TGGAGACTGA TGCCGAACTG GGCAGCCAGC CATCTTTGAC TCCTGAGGAC 
TCAGAGGTTT CCTCCCAATG CCCATCAGCC AGTGGCTCCA GTGGCTCAGA CAGCAGCTGT 
GTGTCAGGGC AGATCCTGGG CAAGGGCCTG GAGGACTTGT CCTACGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHG4 ( XM_005195262.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005195319.1 Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X1, mRNA.

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CloneID	OBa09337
Clone ID Related Accession (Same CDS sequence)	XM_015475823.1 , XM_005218850.2 , XM_015467369.1 , XM_005195262.2
Accession Version	XM_005195262.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	puratrophin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005195262.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGGC CCCTGGAGGA TGGGGATGAG TCTCCAGAGT CCCAGGGCCA TGCCACCGAC 
TGGAGATTTG CTGTGTGCAG TTTCAGAGAT GCCTGGGATG AGGGGGAACC TGCTCCCCAG 
ATGCAGGCTA AGAACCCCCA TCCTCCAAGC CCACCAGCAG GGGCAGCCCA GGGGGAGGAG 
CTCCAGGGCA GCCCTCTGCC TGGTGAACTT CAGTCACCCA CAGGACAGAT GGTTGCAGAT 
GAGTCGGGGC ATGGCCAGAA GGGCACATTA AGAGGATCTT CTGTCCACAT GAAGGAACCA 
GCCTCCTCTG GAGTGAAGAT CCTCCTAAGT CCAATGTCTT CCCACCTTAG TCTGGCACAG 
GGTGAGAATG ACAGCCAAGG AGGAGACTTG GCAGGGGACT CAGTTTCAGG CAGGGTCATG 
CCAGTCCGCT TGGACCCTGA AGTGTTGGAC AGTGACCCTG TGAACCATGG AGGTCTTTTA 
TCTGAGACAT CGTCAGAGCT ACTGGAGGCA GACTCCCACA GATCCAGACT CTTGAAGTCC 
ACTGACTACC TCCTGGCCCA AGACATTGCC TGGGAGCTAC TTGCCAGTGG CATGGCTGCC 
CTGCCAGGGA CTCGAGATGT AGAAGGCCGG GCAGTGCTGC TTCTGTGTGC CCATAACTCA 
GCTTGGAATC ACCCCAAGTG CAACAGCCAT GAGCTCGTCC GCCTCCTGCT CTACTTGCAA 
AGCATCCCCA GGCCCAAAGT ACAGACCTTG GGACTGACCG TGCTAGTGGA CGCCCGGATT 
TGTTCCCCAA GCTCTTCCAT ATTCTGGGGG CTCAGCCAAC TGCAAGAAGC AGTCCCAGGA 
TCAGTGCACC GTGTGCTGCT GGTGGGAAAG CTGCCGGAGG AAGTGCCTGC AGGGCTTCAG 
ATAGAGCATC TATCCTCTCA CCAAAGCCTG CTGACCCACA TCTCCACCTC AGAGTTGCTC 
ACTTCACTAG GAGGAGGCCT GCCTTACTGT CACCAAGCTT GGCTGGACTT CCGGATGCGT 
CTGGAAGCCC TCCTGCAGAG CTGCCAGGTG GTTTGTGCCC TGCTTCAGGG AGCCATTGAG 
AGCATGAAGG CTGTGCCTCA GCCTGTGGAG TCTGGGGAAG TCGGTCAACT GCTGCAGCAG 
GCACGGGTCC TGATGAAGCA TGTGCTAGAC TCTCCACGGT TGGCATGGCT ACAATGCCAG 
GGAGCCTTGG AGCTGACATG GCTAAAGCAA GAGGTCCCAG AGGTGACCCT GAGCCCAGAC 
TACAGGTCAG CAGTGGACGA GGTTGAGGAG CTGTATGGCC GCGTGGATGG ATTGTTGCAC 
CAACTGACAC TGCAGGGTAA CCGCCGAATA CAAGCACTGG AGTTGGTCCA GACGCTGGAG 
GCCCAGGAGG GCGAGCTGCA CCAGATGGAA GTGTGGCTTC AGCAGGTGGG CTGGCCAGCA 
CTTGAGGAGC CCAGGGAGCC TTCACTAGAC GTGCTGCTTC AGGCCCAAGG CCCTTTTCGG 
GAGCTGGACC AGATTGCTCA GGAACAGGTC AGGCGAGGGG AGAAATGTCT GCAGCCACTG 
GTTGGCTGTG ATGCGGCCGA ACTGGGCCCC TTTGGGGCCC GCTTTCTGAC CCTGCAAACC 
CAGCTGGCCG ACTTCTCTAG GGCTTTGGCC CAGCGGCGGC AGCGACTGGC AGATGCTGAG 
AGACTCTTGC AGTTCTTCAA GCAGGCCTTG ACCTGGGCCG AGGAGGGGCG GCAAGTGTTG 
GCAGAGCTGG AACAGGAGCA CCCAGGGGTT GTGCTGCAGC GGCTGCAGCT GCATTGGACC 
AAGTACCCGG ACTTGCCTCC TGCCCACTTC CGAAAGATGT GGGCTTTAGC CACAGGGCTG 
GGATCTGAGG CCATTCGCCA TGAGTGCCGC GGGGCCTGGG CGCAGTGCCA GGACACCTGG 
CTGGCCCTGG ATCAGAAGTT AGAAGCTGCT CTGAAGTCAC CCCCAACGGG TAGCACAGCT 
AGCCTGTGTG TCAGCCGGGT CCCAGCGGTA CCGGCTGTCC CTCCCCTGAG GAAAGCCTAT 
AGCCTTGATC GGAATCTGGG ATTGAGTCTT AGAGAACCTG CCCACCAGTG CCACCATGAG 
GCCATTGTAC CTGCCTGCCA CAGACCAGAG GCTGGAGGTG GTGCCCAGTC AGGGTCATGC 
CCCACCGTGC CTCCTCCAGG GAGCTGTGAC CCCAGGAGCC CCAACAGACT TCAGCTGGTG 
CTGGCGGAGA TGGTGGCTAC GGAGCGGGAG TATGTCCGTG CCCTGGACTA CACCATGGAG 
AACTACTTCC CTGAGCTGGA TCGCCCTGAT GTGCCCCAGG GCCTCCGTGG CCAGCGTGCC 
CAACTCTTTG GCAACCTGGA GAAGCTGTGG GACTTCCACC GCCATTTTTT CTTGCGTGAG 
CTGGAGGCCT GTACCCAGCA CCCGCCCCGG GTGGCCTATG CCTTCCTGCG CCATAGGGTG 
CAGTTTGGGA TGTACGCACT CTACAGCAAG AATAAACCGC GCTCCGATGC CCTGATGACC 
AGCTATGGTC ACGTCTTCTT CAGGGACAAG CAGCAGGCAC TGGGGGACCA CTTGGACTTG 
GCCTCCTACC TGCTGAAGCC CATCCAGCGC ATGAGCAAGT ATGCGCTGCT GCTGCAGGAG 
CTGGCACGGG CCTGCGGGGG ACCCGCACAG GAGCTGAGTG CCCTGCGGGC TGCCCAGAGC 
CTCGTGCACT TCCAGCTGCG ACACGGCAAT GACTTGCTAG CCATGGATGC CATCCAGGGC 
TGTGACGTCA ACCTCAAGGA ACAGGGCCAG TTGGTCCGGC AGGATGAGTT CACAGTCCGC 
TGTGGGCGCC ACAAATCCCT GCGTCGCATT TTCCTCTTTG AGGAGCTGTT GCTCTTCAGC 
AAGCCTCGCC GAGGACCCAC AGGCATTGAC ATGTTTACCT ACAAGCGCTC CTTCAAGATG 
GCAGACCTTG GCCTCACTGA GTGCTGTGGG GACAACAACT TGCGCTTTGA GATCTGGTTT 
CGCCGTCGCA AGGCTAGGGA CACCTTTGTG CTACAAGCTG CCAGCTTGGC CACCAAGCAG 
GCCTGGACAG CTGACATCTC CCGCCTGCTC TGGAGGCAGG CCGTCCACAA CAAGGAGGTG 
CGCATGGCTG AGATGGTGTC TATGGGTGTG GGGAACAAGG CCTTCCGGGA CATCGCACCC 
AGCGAAGAAG CTATCAACGA CCGCACCGTC AACTATATCC TGAAGTGCCA AGCAGTTCGC 
TCTCGGGCCT CCATTGCTGT GGCTCCTCTT GACTCTGACA GCCCCTGCCT GGGGGCCTCA 
GGCACCCTTC CTGGAGATCC TGCCTCTTGC TCTGTACTGG GGTCCCTCAA CCTGCATCTG 
CACAGAGACC CAGCTCTTCT GGGGTTCCGC TGGCCCCTGT ATTCCACCAG TTTCCCAGAG 
GAAGCAGCAC TGGAGACTGA TGCCGAACTG GGCAGCCAGC CATCTTTGAC TCCTGAGGAC 
TCAGAGGTTT CCTCCCAATG CCCATCAGCC AGTGGCTCCA GTGGCTCAGA CAGCAGCTGT 
GTGTCAGGGC AGATCCTGGG CAAGGGCCTG GAGGACTTGT CCTACGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005195319.1
CDS	292..3882
Translation	MEGPLEDGDESPESQGHATDWRFAVCSFRDAWDEGEPAPQMQAKNPHPPSPPAGAAQGEELQGSPLPGELQSPTGQMVADESGHGQKGTLRGSSVHMKEPASSGVKILLSPMSSHLSLAQGENDSQGGDLAGDSVSGRVMPVRLDPEVLDSDPVNHGGLLSETSSELLEADSHRSRLLKSTDYLLAQDIAWELLASGMAALPGTRDVEGRAVLLLCAHNSAWNHPKCNSHELVRLLLYLQSIPRPKVQTLGLTVLVDARICSPSSSIFWGLSQLQEAVPGSVHRVLLVGKLPEEVPAGLQIEHLSSHQSLLTHISTSELLTSLGGGLPYCHQAWLDFRMRLEALLQSCQVVCALLQGAIESMKAVPQPVESGEVGQLLQQARVLMKHVLDSPRLAWLQCQGALELTWLKQEVPEVTLSPDYRSAVDEVEELYGRVDGLLHQLTLQGNRRIQALELVQTLEAQEGELHQMEVWLQQVGWPALEEPREPSLDVLLQAQGPFRELDQIAQEQVRRGEKCLQPLVGCDAAELGPFGARFLTLQTQLADFSRALAQRRQRLADAERLLQFFKQALTWAEEGRQVLAELEQEHPGVVLQRLQLHWTKYPDLPPAHFRKMWALATGLGSEAIRHECRGAWAQCQDTWLALDQKLEAALKSPPTGSTASLCVSRVPAVPAVPPLRKAYSLDRNLGLSLREPAHQCHHEAIVPACHRPEAGGGAQSGSCPTVPPPGSCDPRSPNRLQLVLAEMVATEREYVRALDYTMENYFPELDRPDVPQGLRGQRAQLFGNLEKLWDFHRHFFLRELEACTQHPPRVAYAFLRHRVQFGMYALYSKNKPRSDALMTSYGHVFFRDKQQALGDHLDLASYLLKPIQRMSKYALLLQELARACGGPAQELSALRAAQSLVHFQLRHGNDLLAMDAIQGCDVNLKEQGQLVRQDEFTVRCGRHKSLRRIFLFEELLLFSKPRRGPTGIDMFTYKRSFKMADLGLTECCGDNNLRFEIWFRRRKARDTFVLQAASLATKQAWTADISRLLWRQAVHNKEVRMAEMVSMGVGNKAFRDIAPSEEAINDRTVNYILKCQAVRSRASIAVAPLDSDSPCLGASGTLPGDPASCSVLGSLNLHLHRDPALLGFRWPLYSTSFPEEAALETDAELGSQPSLTPEDSEVSSQCPSASGSSGSDSSCVSGQILGKGLEDLSYV

Target ORF information:

RefSeq Version	XM_005195262.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005195262.2

ORF Insert Sequence:

ATGGAAGGGC CCCTGGAGGA TGGGGATGAG TCTCCAGAGT CCCAGGGCCA TGCCACCGAC 
TGGAGATTTG CTGTGTGCAG TTTCAGAGAT GCCTGGGATG AGGGGGAACC TGCTCCCCAG 
ATGCAGGCTA AGAACCCCCA TCCTCCAAGC CCACCAGCAG GGGCAGCCCA GGGGGAGGAG 
CTCCAGGGCA GCCCTCTGCC TGGTGAACTT CAGTCACCCA CAGGACAGAT GGTTGCAGAT 
GAGTCGGGGC ATGGCCAGAA GGGCACATTA AGAGGATCTT CTGTCCACAT GAAGGAACCA 
GCCTCCTCTG GAGTGAAGAT CCTCCTAAGT CCAATGTCTT CCCACCTTAG TCTGGCACAG 
GGTGAGAATG ACAGCCAAGG AGGAGACTTG GCAGGGGACT CAGTTTCAGG CAGGGTCATG 
CCAGTCCGCT TGGACCCTGA AGTGTTGGAC AGTGACCCTG TGAACCATGG AGGTCTTTTA 
TCTGAGACAT CGTCAGAGCT ACTGGAGGCA GACTCCCACA GATCCAGACT CTTGAAGTCC 
ACTGACTACC TCCTGGCCCA AGACATTGCC TGGGAGCTAC TTGCCAGTGG CATGGCTGCC 
CTGCCAGGGA CTCGAGATGT AGAAGGCCGG GCAGTGCTGC TTCTGTGTGC CCATAACTCA 
GCTTGGAATC ACCCCAAGTG CAACAGCCAT GAGCTCGTCC GCCTCCTGCT CTACTTGCAA 
AGCATCCCCA GGCCCAAAGT ACAGACCTTG GGACTGACCG TGCTAGTGGA CGCCCGGATT 
TGTTCCCCAA GCTCTTCCAT ATTCTGGGGG CTCAGCCAAC TGCAAGAAGC AGTCCCAGGA 
TCAGTGCACC GTGTGCTGCT GGTGGGAAAG CTGCCGGAGG AAGTGCCTGC AGGGCTTCAG 
ATAGAGCATC TATCCTCTCA CCAAAGCCTG CTGACCCACA TCTCCACCTC AGAGTTGCTC 
ACTTCACTAG GAGGAGGCCT GCCTTACTGT CACCAAGCTT GGCTGGACTT CCGGATGCGT 
CTGGAAGCCC TCCTGCAGAG CTGCCAGGTG GTTTGTGCCC TGCTTCAGGG AGCCATTGAG 
AGCATGAAGG CTGTGCCTCA GCCTGTGGAG TCTGGGGAAG TCGGTCAACT GCTGCAGCAG 
GCACGGGTCC TGATGAAGCA TGTGCTAGAC TCTCCACGGT TGGCATGGCT ACAATGCCAG 
GGAGCCTTGG AGCTGACATG GCTAAAGCAA GAGGTCCCAG AGGTGACCCT GAGCCCAGAC 
TACAGGTCAG CAGTGGACGA GGTTGAGGAG CTGTATGGCC GCGTGGATGG ATTGTTGCAC 
CAACTGACAC TGCAGGGTAA CCGCCGAATA CAAGCACTGG AGTTGGTCCA GACGCTGGAG 
GCCCAGGAGG GCGAGCTGCA CCAGATGGAA GTGTGGCTTC AGCAGGTGGG CTGGCCAGCA 
CTTGAGGAGC CCAGGGAGCC TTCACTAGAC GTGCTGCTTC AGGCCCAAGG CCCTTTTCGG 
GAGCTGGACC AGATTGCTCA GGAACAGGTC AGGCGAGGGG AGAAATGTCT GCAGCCACTG 
GTTGGCTGTG ATGCGGCCGA ACTGGGCCCC TTTGGGGCCC GCTTTCTGAC CCTGCAAACC 
CAGCTGGCCG ACTTCTCTAG GGCTTTGGCC CAGCGGCGGC AGCGACTGGC AGATGCTGAG 
AGACTCTTGC AGTTCTTCAA GCAGGCCTTG ACCTGGGCCG AGGAGGGGCG GCAAGTGTTG 
GCAGAGCTGG AACAGGAGCA CCCAGGGGTT GTGCTGCAGC GGCTGCAGCT GCATTGGACC 
AAGTACCCGG ACTTGCCTCC TGCCCACTTC CGAAAGATGT GGGCTTTAGC CACAGGGCTG 
GGATCTGAGG CCATTCGCCA TGAGTGCCGC GGGGCCTGGG CGCAGTGCCA GGACACCTGG 
CTGGCCCTGG ATCAGAAGTT AGAAGCTGCT CTGAAGTCAC CCCCAACGGG TAGCACAGCT 
AGCCTGTGTG TCAGCCGGGT CCCAGCGGTA CCGGCTGTCC CTCCCCTGAG GAAAGCCTAT 
AGCCTTGATC GGAATCTGGG ATTGAGTCTT AGAGAACCTG CCCACCAGTG CCACCATGAG 
GCCATTGTAC CTGCCTGCCA CAGACCAGAG GCTGGAGGTG GTGCCCAGTC AGGGTCATGC 
CCCACCGTGC CTCCTCCAGG GAGCTGTGAC CCCAGGAGCC CCAACAGACT TCAGCTGGTG 
CTGGCGGAGA TGGTGGCTAC GGAGCGGGAG TATGTCCGTG CCCTGGACTA CACCATGGAG 
AACTACTTCC CTGAGCTGGA TCGCCCTGAT GTGCCCCAGG GCCTCCGTGG CCAGCGTGCC 
CAACTCTTTG GCAACCTGGA GAAGCTGTGG GACTTCCACC GCCATTTTTT CTTGCGTGAG 
CTGGAGGCCT GTACCCAGCA CCCGCCCCGG GTGGCCTATG CCTTCCTGCG CCATAGGGTG 
CAGTTTGGGA TGTACGCACT CTACAGCAAG AATAAACCGC GCTCCGATGC CCTGATGACC 
AGCTATGGTC ACGTCTTCTT CAGGGACAAG CAGCAGGCAC TGGGGGACCA CTTGGACTTG 
GCCTCCTACC TGCTGAAGCC CATCCAGCGC ATGAGCAAGT ATGCGCTGCT GCTGCAGGAG 
CTGGCACGGG CCTGCGGGGG ACCCGCACAG GAGCTGAGTG CCCTGCGGGC TGCCCAGAGC 
CTCGTGCACT TCCAGCTGCG ACACGGCAAT GACTTGCTAG CCATGGATGC CATCCAGGGC 
TGTGACGTCA ACCTCAAGGA ACAGGGCCAG TTGGTCCGGC AGGATGAGTT CACAGTCCGC 
TGTGGGCGCC ACAAATCCCT GCGTCGCATT TTCCTCTTTG AGGAGCTGTT GCTCTTCAGC 
AAGCCTCGCC GAGGACCCAC AGGCATTGAC ATGTTTACCT ACAAGCGCTC CTTCAAGATG 
GCAGACCTTG GCCTCACTGA GTGCTGTGGG GACAACAACT TGCGCTTTGA GATCTGGTTT 
CGCCGTCGCA AGGCTAGGGA CACCTTTGTG CTACAAGCTG CCAGCTTGGC CACCAAGCAG 
GCCTGGACAG CTGACATCTC CCGCCTGCTC TGGAGGCAGG CCGTCCACAA CAAGGAGGTG 
CGCATGGCTG AGATGGTGTC TATGGGTGTG GGGAACAAGG CCTTCCGGGA CATCGCACCC 
AGCGAAGAAG CTATCAACGA CCGCACCGTC AACTATATCC TGAAGTGCCA AGCAGTTCGC 
TCTCGGGCCT CCATTGCTGT GGCTCCTCTT GACTCTGACA GCCCCTGCCT GGGGGCCTCA 
GGCACCCTTC CTGGAGATCC TGCCTCTTGC TCTGTACTGG GGTCCCTCAA CCTGCATCTG 
CACAGAGACC CAGCTCTTCT GGGGTTCCGC TGGCCCCTGT ATTCCACCAG TTTCCCAGAG 
GAAGCAGCAC TGGAGACTGA TGCCGAACTG GGCAGCCAGC CATCTTTGAC TCCTGAGGAC 
TCAGAGGTTT CCTCCCAATG CCCATCAGCC AGTGGCTCCA GTGGCTCAGA CAGCAGCTGT 
GTGTCAGGGC AGATCCTGGG CAAGGGCCTG GAGGACTTGT CCTACGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PLEKHG4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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