eIF5B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eIF5B
Entrez Gene ID	44261
Full Name	CG10840 gene product from transcript CG10840-RE
Synonyms	A1,CG10840,CG14970,Dmel\CG10840,Eif5b,IF2,anon-WO0118547.362,cIF2,dIF2,yIF2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 63E1-63E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259671.2	NP_001246600.1	eukaryotic translation initiation factor 5B, isoform D
NM_001259672.2	NP_001246601.1	eukaryotic translation initiation factor 5B, isoform E
NM_001259670.2	NP_001246599.1	eukaryotic translation initiation factor 5B, isoform C
NM_143717.3	NP_651974.1	eukaryotic translation initiation factor 5B, isoform B
NM_001259673.2	NP_001246602.1	eukaryotic translation initiation factor 5B, isoform F

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004824	XP_317984.3
Magnaporthe oryzae (rice blast fungus)	MGG_06066	XP_003711920.1
Neurospora crassa	NCU05270	XP_961727.1
Drosophila melanogaster (fruit fly)	eIF5B	NP_001246602.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

interaction with the general translation factor eIF5B is essential for Vas function during development
Title: Interaction with eIF5B is essential for Vasa function during development.

The following eIF5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eIF5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19373	NM_001259672.2 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa19374	NM_001259671.2 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa19375	NM_001259673.2 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa19375	NM_143717.3 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa19375	NM_001259670.2 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eIF5B ( NM_001259672.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246601.1 Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa19373
Clone ID Related Accession (Same CDS sequence)	NM_001259672.2
Accession Version	NM_001259672.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 5B, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001259672.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT 
GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG 
AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG 
GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC 
GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG 
GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC 
GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG 
GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG 
AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT 
GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG 
GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA 
CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA 
GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG 
AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT 
GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC 
CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG 
GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC 
AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT 
AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC 
GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG 
GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT 
GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT 
GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT 
GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC 
GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG 
GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG 
CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG 
CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT 
CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA 
GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT 
GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA 
TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA 
ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC 
AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC 
AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG 
CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA 
TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT 
ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG 
CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT 
CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC 
ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG 
AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG 
GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC 
GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG 
GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG 
TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC 
GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA 
TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA 
TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG 
AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGTTC 
GTGGACATTG GAATCGTGAC TTCCATCGAA TCGAATCACA AGCAAATCGA ATTCGCGCGC 
AAGGGTCAAG AGATTTGCGT GAAAATCGAT CCGATTCCCG GTGAATCGCC CAAAATGTTT 
GGTCGCCACT TCGAGGCCGA TGATATGCTG ATTAGCAAGA TCTCTCGCCA GAGCATTGAT 
GCCTGCAAGG ATTACTTCCG TGACGATCTG ATCAAGGCGG ATTGGGCGCT GATGGTGGAG 
CTGAAGAAGC TCTTCGAGAT TCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246601.1
CDS	362..3688
Translation	MVKAKKGKKEILTKVEGGSSVDEISDVDSDQLSLNNKQNHALKGNQAGDDDVKAVTKGVKKLNVKGKGKAGKNDDSDEEEVVPAKGKASKKSAFELLMDDDEQDEPAAQESQSEKEEKVVVSKPQKNEKKGKKAKRKGKDDDDEDLDKVLAELQAEYAGEAAPAATTVVSPEELADEFSKKKKNKKQAKQAAVAENAGEDEASDDEEGGSTVKSAAQKKKEKKERQKREAALAKQKAATEPKPKPVEKPAPEPEPVATEEVQPAAEEEEEKSSKNKKKGKKDKKAEPEEEKKDAKKKGMSASMVAAMQEQLRKRKEEEERLEREEAERIRLEDEREEARLEAVRLEAERKEKKKQREAERKARLKAEGKLLTKKQKEDRARAQALLESLKAQGLEIPDPNDKRPTRPGTRIRPNKKKGPKEEAAEEEAKAAEAEAQSAAAAAAAAAAEKAEEERVKESWDATDSEAEPEPEEETSQATNNGKTEAAEEENEDTDDDEDDDDDEDDSDDSDGDSDDSREVSVLANDPESRRLRAEARILKRQAEAEKKRSTDELRAGVVCVLGHVDTGKTKILDKLRRTHVQDSEAGGITQQIGATNVPIEAIKEQTKYVKAAAGFEHRLPGLLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESIQLLKKKKCPFIVALNKIDRLYDWKQLARRDVRDVLKEQQSNTQLEFQQRTKDVILQFAEQGLNAALFYENTDPKTYISLVPTSAISGEGMGNLLFMIADFCQNMLAKRLMYSEELQATVLEVKALPGLGTTIDAILINGKLREGQTMVVAGTDGPIVTQIRSLLMPQPMKELRVKNAYVEYKEVKAAQGVKIAAKDLEKAIAGINLLIAHKPDEVEICTEEVARELKSALSHIKLAQTGVHVQASTLGSLEALLEFLRTSKIPYSAIRIGPVVKRDVMKASTMLEHEAQYATILAFDVKIEREAQEMADSLGVKIFQADIIYHLFDKFTAYREELKQKKREEFRSVAVFPCKLRFVDIGIVTSIESNHKQIEFARKGQEICVKIDPIPGESPKMFGRHFEADDMLISKISRQSIDACKDYFRDDLIKADWALMVELKKLFEIL

Target ORF information:

RefSeq Version	NM_001259672.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259672.2

ORF Insert Sequence:

ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT 
GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG 
AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG 
GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC 
GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG 
GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC 
GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG 
GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG 
AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT 
GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG 
GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA 
CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA 
GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG 
AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT 
GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC 
CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG 
GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC 
AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT 
AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC 
GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG 
GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT 
GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT 
GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT 
GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC 
GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG 
GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG 
CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG 
CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT 
CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA 
GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT 
GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA 
TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA 
ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC 
AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC 
AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG 
CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA 
TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT 
ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG 
CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT 
CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC 
ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG 
AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG 
GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC 
GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG 
GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG 
TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC 
GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA 
TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA 
TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG 
AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGTTC 
GTGGACATTG GAATCGTGAC TTCCATCGAA TCGAATCACA AGCAAATCGA ATTCGCGCGC 
AAGGGTCAAG AGATTTGCGT GAAAATCGAT CCGATTCCCG GTGAATCGCC CAAAATGTTT 
GGTCGCCACT TCGAGGCCGA TGATATGCTG ATTAGCAAGA TCTCTCGCCA GAGCATTGAT 
GCCTGCAAGG ATTACTTCCG TGACGATCTG ATCAAGGCGG ATTGGGCGCT GATGGTGGAG 
CTGAAGAAGC TCTTCGAGAT TCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF5B ( NM_001259671.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246600.1 Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa19374
Clone ID Related Accession (Same CDS sequence)	NM_001259671.2
Accession Version	NM_001259671.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 5B, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001259671.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT 
GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG 
AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG 
GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC 
GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG 
GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC 
GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG 
GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG 
AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT 
GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG 
GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA 
CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA 
GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG 
AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT 
GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC 
CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG 
GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC 
AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT 
AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC 
GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG 
GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT 
GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT 
GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT 
GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC 
GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG 
GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG 
CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG 
CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT 
CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA 
GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT 
GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA 
TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA 
ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC 
AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC 
AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG 
CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA 
TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT 
ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG 
CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT 
CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC 
ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG 
AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG 
GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC 
GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG 
GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG 
TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC 
GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA 
TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA 
TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG 
AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGATA 
CTGCCACAGT TCGTATTCAA CAGCCGAGAT CCGATCGTGA TGGGCGTGAT GGTCGAGAAC 
GGCATTGTCA AAGTGGGCAC TCCAATTTGC GTGCCCAGCA AGGAGATCTC TCGCCAGAGC 
ATTGATGCCT GCAAGGATTA CTTCCGTGAC GATCTGATCA AGGCGGATTG GGCGCTGATG 
GTGGAGCTGA AGAAGCTCTT CGAGATTCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246600.1
CDS	362..3634
Translation	MVKAKKGKKEILTKVEGGSSVDEISDVDSDQLSLNNKQNHALKGNQAGDDDVKAVTKGVKKLNVKGKGKAGKNDDSDEEEVVPAKGKASKKSAFELLMDDDEQDEPAAQESQSEKEEKVVVSKPQKNEKKGKKAKRKGKDDDDEDLDKVLAELQAEYAGEAAPAATTVVSPEELADEFSKKKKNKKQAKQAAVAENAGEDEASDDEEGGSTVKSAAQKKKEKKERQKREAALAKQKAATEPKPKPVEKPAPEPEPVATEEVQPAAEEEEEKSSKNKKKGKKDKKAEPEEEKKDAKKKGMSASMVAAMQEQLRKRKEEEERLEREEAERIRLEDEREEARLEAVRLEAERKEKKKQREAERKARLKAEGKLLTKKQKEDRARAQALLESLKAQGLEIPDPNDKRPTRPGTRIRPNKKKGPKEEAAEEEAKAAEAEAQSAAAAAAAAAAEKAEEERVKESWDATDSEAEPEPEEETSQATNNGKTEAAEEENEDTDDDEDDDDDEDDSDDSDGDSDDSREVSVLANDPESRRLRAEARILKRQAEAEKKRSTDELRAGVVCVLGHVDTGKTKILDKLRRTHVQDSEAGGITQQIGATNVPIEAIKEQTKYVKAAAGFEHRLPGLLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESIQLLKKKKCPFIVALNKIDRLYDWKQLARRDVRDVLKEQQSNTQLEFQQRTKDVILQFAEQGLNAALFYENTDPKTYISLVPTSAISGEGMGNLLFMIADFCQNMLAKRLMYSEELQATVLEVKALPGLGTTIDAILINGKLREGQTMVVAGTDGPIVTQIRSLLMPQPMKELRVKNAYVEYKEVKAAQGVKIAAKDLEKAIAGINLLIAHKPDEVEICTEEVARELKSALSHIKLAQTGVHVQASTLGSLEALLEFLRTSKIPYSAIRIGPVVKRDVMKASTMLEHEAQYATILAFDVKIEREAQEMADSLGVKIFQADIIYHLFDKFTAYREELKQKKREEFRSVAVFPCKLRILPQFVFNSRDPIVMGVMVENGIVKVGTPICVPSKEISRQSIDACKDYFRDDLIKADWALMVELKKLFEIL

Target ORF information:

RefSeq Version	NM_001259671.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259671.2

ORF Insert Sequence:

ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT 
GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG 
AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG 
GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC 
GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG 
GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC 
GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG 
GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG 
AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT 
GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG 
GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA 
CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA 
GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG 
AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT 
GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC 
CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG 
GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC 
AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT 
AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC 
GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG 
GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT 
GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT 
GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT 
GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC 
GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG 
GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG 
CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG 
CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT 
CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA 
GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT 
GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA 
TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA 
ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC 
AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC 
AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG 
CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA 
TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT 
ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG 
CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT 
CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC 
ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG 
AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG 
GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC 
GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG 
GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG 
TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC 
GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA 
TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA 
TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG 
AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGATA 
CTGCCACAGT TCGTATTCAA CAGCCGAGAT CCGATCGTGA TGGGCGTGAT GGTCGAGAAC 
GGCATTGTCA AAGTGGGCAC TCCAATTTGC GTGCCCAGCA AGGAGATCTC TCGCCAGAGC 
ATTGATGCCT GCAAGGATTA CTTCCGTGAC GATCTGATCA AGGCGGATTG GGCGCTGATG 
GTGGAGCTGA AGAAGCTCTT CGAGATTCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF5B ( NM_001259670.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246599.1 Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa19375
Clone ID Related Accession (Same CDS sequence)	NM_001259670.2 , NM_143717.3 , NM_001259673.2
Accession Version	NM_001259670.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 5B, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001259670.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT 
GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG 
AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG 
GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC 
GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG 
GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC 
GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG 
GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG 
AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT 
GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG 
GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA 
CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA 
GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG 
AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT 
GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC 
CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG 
GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC 
AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT 
AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC 
GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG 
GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT 
GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT 
GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT 
GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC 
GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG 
GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG 
CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG 
CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT 
CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA 
GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT 
GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA 
TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA 
ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC 
AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC 
AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG 
CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA 
TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT 
ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG 
CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT 
CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC 
ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG 
AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG 
GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC 
GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG 
GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG 
TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC 
GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA 
TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA 
TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG 
AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGATA 
CTGCCACAGT TCGTATTCAA CAGCCGAGAT CCGATCGTGA TGGGCGTGAT GGTCGAGAAC 
GGCATTGTCA AAGTGGGCAC TCCAATTTGC GTGCCCAGCA AGGAGTTCGT GGACATTGGA 
ATCGTGACTT CCATCGAATC GAATCACAAG CAAATCGAAT TCGCGCGCAA GGGTCAAGAG 
ATTTGCGTGA AAATCGATCC GATTCCCGGT GAATCGCCCA AAATGTTTGG TCGCCACTTC 
GAGGCCGATG ATATGCTGAT TAGCAAGATC TCTCGCCAGA GCATTGATGC CTGCAAGGAT 
TACTTCCGTG ACGATCTGAT CAAGGCGGAT TGGGCGCTGA TGGTGGAGCT GAAGAAGCTC 
TTCGAGATTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246599.1
CDS	633..4067
Translation	MVKAKKGKKEILTKVEGGSSVDEISDVDSDQLSLNNKQNHALKGNQAGDDDVKAVTKGVKKLNVKGKGKAGKNDDSDEEEVVPAKGKASKKSAFELLMDDDEQDEPAAQESQSEKEEKVVVSKPQKNEKKGKKAKRKGKDDDDEDLDKVLAELQAEYAGEAAPAATTVVSPEELADEFSKKKKNKKQAKQAAVAENAGEDEASDDEEGGSTVKSAAQKKKEKKERQKREAALAKQKAATEPKPKPVEKPAPEPEPVATEEVQPAAEEEEEKSSKNKKKGKKDKKAEPEEEKKDAKKKGMSASMVAAMQEQLRKRKEEEERLEREEAERIRLEDEREEARLEAVRLEAERKEKKKQREAERKARLKAEGKLLTKKQKEDRARAQALLESLKAQGLEIPDPNDKRPTRPGTRIRPNKKKGPKEEAAEEEAKAAEAEAQSAAAAAAAAAAEKAEEERVKESWDATDSEAEPEPEEETSQATNNGKTEAAEEENEDTDDDEDDDDDEDDSDDSDGDSDDSREVSVLANDPESRRLRAEARILKRQAEAEKKRSTDELRAGVVCVLGHVDTGKTKILDKLRRTHVQDSEAGGITQQIGATNVPIEAIKEQTKYVKAAAGFEHRLPGLLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESIQLLKKKKCPFIVALNKIDRLYDWKQLARRDVRDVLKEQQSNTQLEFQQRTKDVILQFAEQGLNAALFYENTDPKTYISLVPTSAISGEGMGNLLFMIADFCQNMLAKRLMYSEELQATVLEVKALPGLGTTIDAILINGKLREGQTMVVAGTDGPIVTQIRSLLMPQPMKELRVKNAYVEYKEVKAAQGVKIAAKDLEKAIAGINLLIAHKPDEVEICTEEVARELKSALSHIKLAQTGVHVQASTLGSLEALLEFLRTSKIPYSAIRIGPVVKRDVMKASTMLEHEAQYATILAFDVKIEREAQEMADSLGVKIFQADIIYHLFDKFTAYREELKQKKREEFRSVAVFPCKLRILPQFVFNSRDPIVMGVMVENGIVKVGTPICVPSKEFVDIGIVTSIESNHKQIEFARKGQEICVKIDPIPGESPKMFGRHFEADDMLISKISRQSIDACKDYFRDDLIKADWALMVELKKLFEIL

Target ORF information:

RefSeq Version	NM_001259670.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259670.2

ORF Insert Sequence:

ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT 
GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG 
AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG 
GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC 
GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG 
GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC 
GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG 
GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG 
AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT 
GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG 
GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA 
CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA 
GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG 
AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT 
GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC 
CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG 
GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC 
AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT 
AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC 
GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG 
GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT 
GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT 
GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT 
GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC 
GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG 
GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG 
CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG 
CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT 
CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA 
GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT 
GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA 
TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA 
ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC 
AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC 
AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG 
CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA 
TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT 
ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG 
CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT 
CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC 
ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG 
AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG 
GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC 
GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG 
GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG 
TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC 
GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA 
TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA 
TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG 
AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGATA 
CTGCCACAGT TCGTATTCAA CAGCCGAGAT CCGATCGTGA TGGGCGTGAT GGTCGAGAAC 
GGCATTGTCA AAGTGGGCAC TCCAATTTGC GTGCCCAGCA AGGAGTTCGT GGACATTGGA 
ATCGTGACTT CCATCGAATC GAATCACAAG CAAATCGAAT TCGCGCGCAA GGGTCAAGAG 
ATTTGCGTGA AAATCGATCC GATTCCCGGT GAATCGCCCA AAATGTTTGG TCGCCACTTC 
GAGGCCGATG ATATGCTGAT TAGCAAGATC TCTCGCCAGA GCATTGATGC CTGCAAGGAT 
TACTTCCGTG ACGATCTGAT CAAGGCGGAT TGGGCGCTGA TGGTGGAGCT GAAGAAGCTC 
TTCGAGATTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF5B ( NM_143717.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651974.1 Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa19375
Clone ID Related Accession (Same CDS sequence)	NM_001259670.2 , NM_143717.3 , NM_001259673.2
Accession Version	NM_143717.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 5B, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_143717.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT 
GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG 
AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG 
GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC 
GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG 
GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC 
GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG 
GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG 
AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT 
GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG 
GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA 
CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA 
GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG 
AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT 
GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC 
CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG 
GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC 
AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT 
AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC 
GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG 
GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT 
GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT 
GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT 
GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC 
GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG 
GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG 
CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG 
CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT 
CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA 
GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT 
GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA 
TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA 
ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC 
AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC 
AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG 
CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA 
TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT 
ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG 
CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT 
CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC 
ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG 
AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG 
GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC 
GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG 
GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG 
TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC 
GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA 
TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA 
TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG 
AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGATA 
CTGCCACAGT TCGTATTCAA CAGCCGAGAT CCGATCGTGA TGGGCGTGAT GGTCGAGAAC 
GGCATTGTCA AAGTGGGCAC TCCAATTTGC GTGCCCAGCA AGGAGTTCGT GGACATTGGA 
ATCGTGACTT CCATCGAATC GAATCACAAG CAAATCGAAT TCGCGCGCAA GGGTCAAGAG 
ATTTGCGTGA AAATCGATCC GATTCCCGGT GAATCGCCCA AAATGTTTGG TCGCCACTTC 
GAGGCCGATG ATATGCTGAT TAGCAAGATC TCTCGCCAGA GCATTGATGC CTGCAAGGAT 
TACTTCCGTG ACGATCTGAT CAAGGCGGAT TGGGCGCTGA TGGTGGAGCT GAAGAAGCTC 
TTCGAGATTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651974.1
CDS	362..3796
Translation	MVKAKKGKKEILTKVEGGSSVDEISDVDSDQLSLNNKQNHALKGNQAGDDDVKAVTKGVKKLNVKGKGKAGKNDDSDEEEVVPAKGKASKKSAFELLMDDDEQDEPAAQESQSEKEEKVVVSKPQKNEKKGKKAKRKGKDDDDEDLDKVLAELQAEYAGEAAPAATTVVSPEELADEFSKKKKNKKQAKQAAVAENAGEDEASDDEEGGSTVKSAAQKKKEKKERQKREAALAKQKAATEPKPKPVEKPAPEPEPVATEEVQPAAEEEEEKSSKNKKKGKKDKKAEPEEEKKDAKKKGMSASMVAAMQEQLRKRKEEEERLEREEAERIRLEDEREEARLEAVRLEAERKEKKKQREAERKARLKAEGKLLTKKQKEDRARAQALLESLKAQGLEIPDPNDKRPTRPGTRIRPNKKKGPKEEAAEEEAKAAEAEAQSAAAAAAAAAAEKAEEERVKESWDATDSEAEPEPEEETSQATNNGKTEAAEEENEDTDDDEDDDDDEDDSDDSDGDSDDSREVSVLANDPESRRLRAEARILKRQAEAEKKRSTDELRAGVVCVLGHVDTGKTKILDKLRRTHVQDSEAGGITQQIGATNVPIEAIKEQTKYVKAAAGFEHRLPGLLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESIQLLKKKKCPFIVALNKIDRLYDWKQLARRDVRDVLKEQQSNTQLEFQQRTKDVILQFAEQGLNAALFYENTDPKTYISLVPTSAISGEGMGNLLFMIADFCQNMLAKRLMYSEELQATVLEVKALPGLGTTIDAILINGKLREGQTMVVAGTDGPIVTQIRSLLMPQPMKELRVKNAYVEYKEVKAAQGVKIAAKDLEKAIAGINLLIAHKPDEVEICTEEVARELKSALSHIKLAQTGVHVQASTLGSLEALLEFLRTSKIPYSAIRIGPVVKRDVMKASTMLEHEAQYATILAFDVKIEREAQEMADSLGVKIFQADIIYHLFDKFTAYREELKQKKREEFRSVAVFPCKLRILPQFVFNSRDPIVMGVMVENGIVKVGTPICVPSKEFVDIGIVTSIESNHKQIEFARKGQEICVKIDPIPGESPKMFGRHFEADDMLISKISRQSIDACKDYFRDDLIKADWALMVELKKLFEIL

Target ORF information:

RefSeq Version	NM_143717.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143717.3

ORF Insert Sequence:

ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT 
GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG 
AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG 
GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC 
GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG 
GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC 
GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG 
GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG 
AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT 
GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG 
GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA 
CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA 
GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG 
AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT 
GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC 
CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG 
GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC 
AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT 
AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC 
GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG 
GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT 
GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT 
GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT 
GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC 
GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG 
GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG 
CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG 
CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT 
CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA 
GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT 
GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA 
TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA 
ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC 
AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC 
AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG 
CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA 
TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT 
ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG 
CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT 
CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC 
ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG 
AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG 
GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC 
GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG 
GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG 
TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC 
GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA 
TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA 
TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG 
AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGATA 
CTGCCACAGT TCGTATTCAA CAGCCGAGAT CCGATCGTGA TGGGCGTGAT GGTCGAGAAC 
GGCATTGTCA AAGTGGGCAC TCCAATTTGC GTGCCCAGCA AGGAGTTCGT GGACATTGGA 
ATCGTGACTT CCATCGAATC GAATCACAAG CAAATCGAAT TCGCGCGCAA GGGTCAAGAG 
ATTTGCGTGA AAATCGATCC GATTCCCGGT GAATCGCCCA AAATGTTTGG TCGCCACTTC 
GAGGCCGATG ATATGCTGAT TAGCAAGATC TCTCGCCAGA GCATTGATGC CTGCAAGGAT 
TACTTCCGTG ACGATCTGAT CAAGGCGGAT TGGGCGCTGA TGGTGGAGCT GAAGAAGCTC 
TTCGAGATTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF5B ( NM_001259673.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246602.1 Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant F, mRNA.

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CloneID	OFa19375
Clone ID Related Accession (Same CDS sequence)	NM_001259670.2 , NM_143717.3 , NM_001259673.2
Accession Version	NM_001259673.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 5B, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001259673.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT 
GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG 
AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG 
GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC 
GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG 
GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC 
GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG 
GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG 
AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT 
GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG 
GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA 
CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA 
GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG 
AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT 
GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC 
CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG 
GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC 
AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT 
AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC 
GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG 
GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT 
GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT 
GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT 
GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC 
GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG 
GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG 
CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG 
CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT 
CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA 
GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT 
GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA 
TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA 
ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC 
AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC 
AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG 
CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA 
TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT 
ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG 
CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT 
CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC 
ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG 
AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG 
GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC 
GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG 
GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG 
TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC 
GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA 
TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA 
TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG 
AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGATA 
CTGCCACAGT TCGTATTCAA CAGCCGAGAT CCGATCGTGA TGGGCGTGAT GGTCGAGAAC 
GGCATTGTCA AAGTGGGCAC TCCAATTTGC GTGCCCAGCA AGGAGTTCGT GGACATTGGA 
ATCGTGACTT CCATCGAATC GAATCACAAG CAAATCGAAT TCGCGCGCAA GGGTCAAGAG 
ATTTGCGTGA AAATCGATCC GATTCCCGGT GAATCGCCCA AAATGTTTGG TCGCCACTTC 
GAGGCCGATG ATATGCTGAT TAGCAAGATC TCTCGCCAGA GCATTGATGC CTGCAAGGAT 
TACTTCCGTG ACGATCTGAT CAAGGCGGAT TGGGCGCTGA TGGTGGAGCT GAAGAAGCTC 
TTCGAGATTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246602.1
CDS	658..4092
Translation	MVKAKKGKKEILTKVEGGSSVDEISDVDSDQLSLNNKQNHALKGNQAGDDDVKAVTKGVKKLNVKGKGKAGKNDDSDEEEVVPAKGKASKKSAFELLMDDDEQDEPAAQESQSEKEEKVVVSKPQKNEKKGKKAKRKGKDDDDEDLDKVLAELQAEYAGEAAPAATTVVSPEELADEFSKKKKNKKQAKQAAVAENAGEDEASDDEEGGSTVKSAAQKKKEKKERQKREAALAKQKAATEPKPKPVEKPAPEPEPVATEEVQPAAEEEEEKSSKNKKKGKKDKKAEPEEEKKDAKKKGMSASMVAAMQEQLRKRKEEEERLEREEAERIRLEDEREEARLEAVRLEAERKEKKKQREAERKARLKAEGKLLTKKQKEDRARAQALLESLKAQGLEIPDPNDKRPTRPGTRIRPNKKKGPKEEAAEEEAKAAEAEAQSAAAAAAAAAAEKAEEERVKESWDATDSEAEPEPEEETSQATNNGKTEAAEEENEDTDDDEDDDDDEDDSDDSDGDSDDSREVSVLANDPESRRLRAEARILKRQAEAEKKRSTDELRAGVVCVLGHVDTGKTKILDKLRRTHVQDSEAGGITQQIGATNVPIEAIKEQTKYVKAAAGFEHRLPGLLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESIQLLKKKKCPFIVALNKIDRLYDWKQLARRDVRDVLKEQQSNTQLEFQQRTKDVILQFAEQGLNAALFYENTDPKTYISLVPTSAISGEGMGNLLFMIADFCQNMLAKRLMYSEELQATVLEVKALPGLGTTIDAILINGKLREGQTMVVAGTDGPIVTQIRSLLMPQPMKELRVKNAYVEYKEVKAAQGVKIAAKDLEKAIAGINLLIAHKPDEVEICTEEVARELKSALSHIKLAQTGVHVQASTLGSLEALLEFLRTSKIPYSAIRIGPVVKRDVMKASTMLEHEAQYATILAFDVKIEREAQEMADSLGVKIFQADIIYHLFDKFTAYREELKQKKREEFRSVAVFPCKLRILPQFVFNSRDPIVMGVMVENGIVKVGTPICVPSKEFVDIGIVTSIESNHKQIEFARKGQEICVKIDPIPGESPKMFGRHFEADDMLISKISRQSIDACKDYFRDDLIKADWALMVELKKLFEIL

Target ORF information:

RefSeq Version	NM_001259673.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259673.2

ORF Insert Sequence:

ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT 
GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG 
AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG 
GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC 
GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG 
GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC 
GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG 
GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG 
AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT 
GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG 
GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA 
CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA 
GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG 
AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT 
GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC 
CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG 
GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC 
AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT 
AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC 
GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG 
GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT 
GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT 
GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT 
GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC 
GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG 
GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG 
CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG 
CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT 
CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA 
GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT 
GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA 
TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA 
ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC 
AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC 
AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG 
CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA 
TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT 
ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG 
CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT 
CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC 
ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG 
AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG 
GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC 
GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG 
GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG 
TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC 
GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA 
TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA 
TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG 
AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGATA 
CTGCCACAGT TCGTATTCAA CAGCCGAGAT CCGATCGTGA TGGGCGTGAT GGTCGAGAAC 
GGCATTGTCA AAGTGGGCAC TCCAATTTGC GTGCCCAGCA AGGAGTTCGT GGACATTGGA 
ATCGTGACTT CCATCGAATC GAATCACAAG CAAATCGAAT TCGCGCGCAA GGGTCAAGAG 
ATTTGCGTGA AAATCGATCC GATTCCCGGT GAATCGCCCA AAATGTTTGG TCGCCACTTC 
GAGGCCGATG ATATGCTGAT TAGCAAGATC TCTCGCCAGA GCATTGATGC CTGCAAGGAT 
TACTTCCGTG ACGATCTGAT CAAGGCGGAT TGGGCGCTGA TGGTGGAGCT GAAGAAGCTC 
TTCGAGATTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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eIF5B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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