eIF5B cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following eIF5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eIF5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa19373 NM_001259672.2
Latest version!
Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa19374 NM_001259671.2
Latest version!
Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa19375 NM_001259673.2
Latest version!
Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa19375 NM_143717.3
Latest version!
Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa19375 NM_001259670.2
Latest version!
Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa19373
    Clone ID Related Accession (Same CDS sequence) NM_001259672.2
    Accession Version NM_001259672.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product eukaryotic translation initiation factor 5B, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001259672.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
    GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT
    GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG
    AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG
    GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC
    GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG
    GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC
    GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG
    GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG
    AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT
    GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG
    GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA
    CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA
    GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG
    AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT
    GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC
    CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG
    GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC
    AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT
    AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC
    GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG
    GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT
    GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT
    GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT
    GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC
    GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG
    GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG
    CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG
    CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT
    CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA
    GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT
    GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA
    TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA
    ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC
    AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC
    AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG
    CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA
    TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT
    ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG
    CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT
    CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC
    ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG
    AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG
    GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC
    GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG
    GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG
    TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC
    GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA
    TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA
    TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG
    AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGTTC
    GTGGACATTG GAATCGTGAC TTCCATCGAA TCGAATCACA AGCAAATCGA ATTCGCGCGC
    AAGGGTCAAG AGATTTGCGT GAAAATCGAT CCGATTCCCG GTGAATCGCC CAAAATGTTT
    GGTCGCCACT TCGAGGCCGA TGATATGCTG ATTAGCAAGA TCTCTCGCCA GAGCATTGAT
    GCCTGCAAGG ATTACTTCCG TGACGATCTG ATCAAGGCGG ATTGGGCGCT GATGGTGGAG
    CTGAAGAAGC TCTTCGAGAT TCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246601.1
    CDS362..3688
    Translation

    Target ORF information:

    RefSeq Version NM_001259672.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259672.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
    GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT
    GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG
    AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG
    GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC
    GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG
    GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC
    GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG
    GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG
    AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT
    GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG
    GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA
    CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA
    GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG
    AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT
    GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC
    CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG
    GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC
    AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT
    AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC
    GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG
    GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT
    GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT
    GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT
    GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC
    GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG
    GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG
    CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG
    CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT
    CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA
    GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT
    GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA
    TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA
    ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC
    AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC
    AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG
    CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA
    TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT
    ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG
    CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT
    CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC
    ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG
    AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG
    GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC
    GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG
    GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG
    TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC
    GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA
    TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA
    TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG
    AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGTTC
    GTGGACATTG GAATCGTGAC TTCCATCGAA TCGAATCACA AGCAAATCGA ATTCGCGCGC
    AAGGGTCAAG AGATTTGCGT GAAAATCGAT CCGATTCCCG GTGAATCGCC CAAAATGTTT
    GGTCGCCACT TCGAGGCCGA TGATATGCTG ATTAGCAAGA TCTCTCGCCA GAGCATTGAT
    GCCTGCAAGG ATTACTTCCG TGACGATCTG ATCAAGGCGG ATTGGGCGCT GATGGTGGAG
    CTGAAGAAGC TCTTCGAGAT TCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa19374
    Clone ID Related Accession (Same CDS sequence) NM_001259671.2
    Accession Version NM_001259671.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product eukaryotic translation initiation factor 5B, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001259671.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
    GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT
    GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG
    AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG
    GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC
    GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG
    GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC
    GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG
    GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG
    AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT
    GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG
    GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA
    CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA
    GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG
    AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT
    GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC
    CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG
    GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC
    AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT
    AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC
    GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG
    GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT
    GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT
    GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT
    GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC
    GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG
    GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG
    CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG
    CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT
    CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA
    GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT
    GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA
    TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA
    ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC
    AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC
    AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG
    CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA
    TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT
    ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG
    CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT
    CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC
    ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG
    AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG
    GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC
    GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG
    GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG
    TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC
    GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA
    TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA
    TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG
    AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGATA
    CTGCCACAGT TCGTATTCAA CAGCCGAGAT CCGATCGTGA TGGGCGTGAT GGTCGAGAAC
    GGCATTGTCA AAGTGGGCAC TCCAATTTGC GTGCCCAGCA AGGAGATCTC TCGCCAGAGC
    ATTGATGCCT GCAAGGATTA CTTCCGTGAC GATCTGATCA AGGCGGATTG GGCGCTGATG
    GTGGAGCTGA AGAAGCTCTT CGAGATTCTC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246600.1
    CDS362..3634
    Translation

    Target ORF information:

    RefSeq Version NM_001259671.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259671.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
    GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT
    GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG
    AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG
    GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC
    GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG
    GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC
    GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG
    GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG
    AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT
    GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG
    GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA
    CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA
    GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG
    AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT
    GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC
    CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG
    GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC
    AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT
    AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC
    GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG
    GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT
    GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT
    GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT
    GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC
    GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG
    GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG
    CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG
    CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT
    CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA
    GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT
    GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA
    TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA
    ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC
    AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC
    AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG
    CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA
    TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT
    ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG
    CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT
    CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC
    ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG
    AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG
    GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC
    GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG
    GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG
    TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC
    GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA
    TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA
    TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG
    AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGATA
    CTGCCACAGT TCGTATTCAA CAGCCGAGAT CCGATCGTGA TGGGCGTGAT GGTCGAGAAC
    GGCATTGTCA AAGTGGGCAC TCCAATTTGC GTGCCCAGCA AGGAGATCTC TCGCCAGAGC
    ATTGATGCCT GCAAGGATTA CTTCCGTGAC GATCTGATCA AGGCGGATTG GGCGCTGATG
    GTGGAGCTGA AGAAGCTCTT CGAGATTCTC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa19375
    Clone ID Related Accession (Same CDS sequence) NM_001259670.2 , NM_143717.3 , NM_001259673.2
    Accession Version NM_001259670.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3435bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product eukaryotic translation initiation factor 5B, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001259670.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
    GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT
    GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG
    AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG
    GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC
    GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG
    GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC
    GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG
    GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG
    AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT
    GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG
    GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA
    CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA
    GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG
    AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT
    GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC
    CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG
    GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC
    AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT
    AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC
    GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG
    GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT
    GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT
    GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT
    GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC
    GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG
    GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG
    CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG
    CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT
    CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA
    GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT
    GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA
    TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA
    ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC
    AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC
    AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG
    CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA
    TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT
    ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG
    CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT
    CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC
    ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG
    AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG
    GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC
    GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG
    GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG
    TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC
    GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA
    TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA
    TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG
    AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGATA
    CTGCCACAGT TCGTATTCAA CAGCCGAGAT CCGATCGTGA TGGGCGTGAT GGTCGAGAAC
    GGCATTGTCA AAGTGGGCAC TCCAATTTGC GTGCCCAGCA AGGAGTTCGT GGACATTGGA
    ATCGTGACTT CCATCGAATC GAATCACAAG CAAATCGAAT TCGCGCGCAA GGGTCAAGAG
    ATTTGCGTGA AAATCGATCC GATTCCCGGT GAATCGCCCA AAATGTTTGG TCGCCACTTC
    GAGGCCGATG ATATGCTGAT TAGCAAGATC TCTCGCCAGA GCATTGATGC CTGCAAGGAT
    TACTTCCGTG ACGATCTGAT CAAGGCGGAT TGGGCGCTGA TGGTGGAGCT GAAGAAGCTC
    TTCGAGATTC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246599.1
    CDS633..4067
    Translation

    Target ORF information:

    RefSeq Version NM_001259670.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259670.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
    GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT
    GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG
    AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG
    GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC
    GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG
    GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC
    GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG
    GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG
    AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT
    GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG
    GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA
    CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA
    GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG
    AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT
    GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC
    CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG
    GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC
    AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT
    AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC
    GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG
    GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT
    GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT
    GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT
    GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC
    GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG
    GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG
    CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG
    CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT
    CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA
    GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT
    GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA
    TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA
    ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC
    AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC
    AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG
    CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA
    TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT
    ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG
    CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT
    CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC
    ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG
    AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG
    GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC
    GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG
    GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG
    TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC
    GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA
    TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA
    TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG
    AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGATA
    CTGCCACAGT TCGTATTCAA CAGCCGAGAT CCGATCGTGA TGGGCGTGAT GGTCGAGAAC
    GGCATTGTCA AAGTGGGCAC TCCAATTTGC GTGCCCAGCA AGGAGTTCGT GGACATTGGA
    ATCGTGACTT CCATCGAATC GAATCACAAG CAAATCGAAT TCGCGCGCAA GGGTCAAGAG
    ATTTGCGTGA AAATCGATCC GATTCCCGGT GAATCGCCCA AAATGTTTGG TCGCCACTTC
    GAGGCCGATG ATATGCTGAT TAGCAAGATC TCTCGCCAGA GCATTGATGC CTGCAAGGAT
    TACTTCCGTG ACGATCTGAT CAAGGCGGAT TGGGCGCTGA TGGTGGAGCT GAAGAAGCTC
    TTCGAGATTC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa19375
    Clone ID Related Accession (Same CDS sequence) NM_001259670.2 , NM_143717.3 , NM_001259673.2
    Accession Version NM_143717.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3435bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product eukaryotic translation initiation factor 5B, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_143717.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
    GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT
    GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG
    AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG
    GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC
    GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG
    GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC
    GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG
    GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG
    AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT
    GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG
    GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA
    CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA
    GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG
    AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT
    GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC
    CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG
    GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC
    AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT
    AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC
    GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG
    GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT
    GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT
    GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT
    GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC
    GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG
    GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG
    CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG
    CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT
    CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA
    GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT
    GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA
    TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA
    ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC
    AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC
    AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG
    CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA
    TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT
    ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG
    CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT
    CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC
    ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG
    AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG
    GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC
    GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG
    GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG
    TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC
    GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA
    TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA
    TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG
    AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGATA
    CTGCCACAGT TCGTATTCAA CAGCCGAGAT CCGATCGTGA TGGGCGTGAT GGTCGAGAAC
    GGCATTGTCA AAGTGGGCAC TCCAATTTGC GTGCCCAGCA AGGAGTTCGT GGACATTGGA
    ATCGTGACTT CCATCGAATC GAATCACAAG CAAATCGAAT TCGCGCGCAA GGGTCAAGAG
    ATTTGCGTGA AAATCGATCC GATTCCCGGT GAATCGCCCA AAATGTTTGG TCGCCACTTC
    GAGGCCGATG ATATGCTGAT TAGCAAGATC TCTCGCCAGA GCATTGATGC CTGCAAGGAT
    TACTTCCGTG ACGATCTGAT CAAGGCGGAT TGGGCGCTGA TGGTGGAGCT GAAGAAGCTC
    TTCGAGATTC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_651974.1
    CDS362..3796
    Translation

    Target ORF information:

    RefSeq Version NM_143717.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_143717.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
    GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT
    GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG
    AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG
    GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC
    GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG
    GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC
    GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG
    GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG
    AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT
    GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG
    GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA
    CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA
    GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG
    AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT
    GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC
    CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG
    GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC
    AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT
    AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC
    GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG
    GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT
    GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT
    GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT
    GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC
    GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG
    GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG
    CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG
    CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT
    CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA
    GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT
    GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA
    TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA
    ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC
    AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC
    AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG
    CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA
    TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT
    ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG
    CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT
    CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC
    ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG
    AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG
    GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC
    GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG
    GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG
    TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC
    GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA
    TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA
    TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG
    AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGATA
    CTGCCACAGT TCGTATTCAA CAGCCGAGAT CCGATCGTGA TGGGCGTGAT GGTCGAGAAC
    GGCATTGTCA AAGTGGGCAC TCCAATTTGC GTGCCCAGCA AGGAGTTCGT GGACATTGGA
    ATCGTGACTT CCATCGAATC GAATCACAAG CAAATCGAAT TCGCGCGCAA GGGTCAAGAG
    ATTTGCGTGA AAATCGATCC GATTCCCGGT GAATCGCCCA AAATGTTTGG TCGCCACTTC
    GAGGCCGATG ATATGCTGAT TAGCAAGATC TCTCGCCAGA GCATTGATGC CTGCAAGGAT
    TACTTCCGTG ACGATCTGAT CAAGGCGGAT TGGGCGCTGA TGGTGGAGCT GAAGAAGCTC
    TTCGAGATTC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa19375
    Clone ID Related Accession (Same CDS sequence) NM_001259670.2 , NM_143717.3 , NM_001259673.2
    Accession Version NM_001259673.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3435bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product eukaryotic translation initiation factor 5B, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001259673.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
    GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT
    GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG
    AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG
    GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC
    GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG
    GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC
    GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG
    GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG
    AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT
    GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG
    GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA
    CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA
    GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG
    AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT
    GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC
    CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG
    GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC
    AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT
    AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC
    GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG
    GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT
    GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT
    GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT
    GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC
    GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG
    GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG
    CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG
    CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT
    CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA
    GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT
    GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA
    TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA
    ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC
    AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC
    AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG
    CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA
    TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT
    ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG
    CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT
    CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC
    ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG
    AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG
    GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC
    GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG
    GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG
    TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC
    GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA
    TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA
    TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG
    AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGATA
    CTGCCACAGT TCGTATTCAA CAGCCGAGAT CCGATCGTGA TGGGCGTGAT GGTCGAGAAC
    GGCATTGTCA AAGTGGGCAC TCCAATTTGC GTGCCCAGCA AGGAGTTCGT GGACATTGGA
    ATCGTGACTT CCATCGAATC GAATCACAAG CAAATCGAAT TCGCGCGCAA GGGTCAAGAG
    ATTTGCGTGA AAATCGATCC GATTCCCGGT GAATCGCCCA AAATGTTTGG TCGCCACTTC
    GAGGCCGATG ATATGCTGAT TAGCAAGATC TCTCGCCAGA GCATTGATGC CTGCAAGGAT
    TACTTCCGTG ACGATCTGAT CAAGGCGGAT TGGGCGCTGA TGGTGGAGCT GAAGAAGCTC
    TTCGAGATTC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246602.1
    CDS658..4092
    Translation

    Target ORF information:

    RefSeq Version NM_001259673.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster eukaryotic translation initiation factor 5B (eIF5B), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259673.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTAAAG CTAAAAAGGG CAAGAAAGAG ATACTGACCA AGGTCGAAGG CGGTTCCTCG 
    GTGGACGAAA TCTCCGATGT GGACAGCGAC CAGTTGAGCC TCAACAACAA GCAGAATCAT
    GCCCTGAAGG GCAATCAAGC TGGCGATGAC GATGTTAAAG CCGTGACCAA GGGGGTCAAG
    AAGCTTAATG TCAAGGGAAA AGGCAAAGCA GGCAAAAATG ACGACTCCGA TGAGGAGGAG
    GTGGTGCCCG CCAAGGGCAA AGCTTCTAAG AAGTCGGCTT TTGAGCTGCT AATGGACGAC
    GACGAGCAGG ACGAGCCAGC TGCCCAGGAA AGCCAGTCTG AGAAGGAGGA AAAGGTAGTG
    GTCTCCAAGC CACAGAAGAA CGAAAAGAAG GGCAAAAAGG CCAAACGCAA GGGGAAGGAC
    GACGACGATG AAGATCTGGA CAAGGTTTTA GCTGAGCTTC AAGCGGAATA CGCCGGCGAG
    GCTGCTCCTG CTGCCACTAC TGTTGTTTCC CCCGAAGAAT TGGCCGATGA GTTCTCCAAG
    AAAAAAAAGA ACAAGAAGCA GGCCAAGCAG GCTGCAGTGG CAGAAAACGC CGGAGAGGAT
    GAGGCCAGCG ATGACGAGGA GGGCGGCAGC ACTGTGAAGT CGGCAGCCCA GAAAAAGAAG
    GAAAAGAAGG AGCGCCAAAA GAGGGAGGCT GCCCTAGCCA AGCAAAAGGC AGCTACTGAA
    CCCAAACCGA AGCCCGTGGA GAAACCTGCG CCAGAACCCG AGCCTGTGGC TACGGAAGAA
    GTCCAGCCCG CCGCCGAGGA AGAGGAGGAA AAGTCCAGCA AAAACAAGAA GAAGGGCAAG
    AAAGACAAAA AGGCCGAGCC TGAGGAGGAG AAGAAGGACG CCAAGAAGAA GGGTATGTCT
    GCCTCCATGG TGGCGGCCAT GCAGGAGCAG CTAAGAAAGC GAAAGGAGGA GGAGGAGCGC
    CTGGAGCGGG AGGAGGCAGA GCGAATTCGC CTGGAGGATG AGCGCGAAGA AGCCCGCCTG
    GAGGCAGTGC GTCTGGAGGC AGAGAGGAAG GAGAAAAAGA AGCAGCGCGA GGCAGAGCGC
    AAGGCTCGCT TGAAGGCGGA AGGAAAACTG CTCACCAAGA AGCAGAAGGA GGATAGGGCT
    AGGGCACAGG CTCTCCTGGA GTCTCTAAAG GCTCAGGGTC TCGAGATTCC CGATCCGAAC
    GACAAGCGAC CCACGCGGCC AGGAACTCGT ATCCGTCCGA ACAAAAAGAA GGGCCCTAAG
    GAAGAGGCCG CCGAAGAGGA GGCCAAGGCA GCGGAAGCGG AAGCCCAATC GGCCGCCGCT
    GCTGCCGCTG CAGCTGCTGC CGAAAAGGCC GAGGAAGAGA GGGTCAAGGA GAGTTGGGAT
    GCCACCGATA GTGAGGCTGA GCCAGAGCCC GAGGAAGAAA CGTCGCAGGC CACTAACAAT
    GGCAAGACCG AGGCTGCCGA GGAAGAGAAC GAAGATACAG ATGACGATGA AGATGACGAC
    GATGATGAAG ATGACAGCGA TGACTCCGAT GGGGATAGTG ACGATTCCCG TGAAGTATCG
    GTGTTGGCCA ATGATCCAGA GTCACGACGA CTGCGTGCGG AAGCCAGAAT CCTAAAACGG
    CAAGCAGAGG CGGAAAAGAA GCGCTCCACC GACGAACTCA GAGCTGGAGT GGTTTGCGTG
    CTGGGTCATG TAGATACAGG AAAAACCAAG ATCCTGGACA AACTGCGGCG CACACACGTT
    CAGGATTCCG AAGCGGGTGG CATCACTCAG CAGATTGGAG CCACAAACGT TCCCATCGAA
    GCAATTAAGG AGCAGACTAA GTACGTAAAG GCGGCCGCTG GATTTGAACA TCGCTTGCCT
    GGTCTATTGA TCATCGACAC ACCCGGCCAC GAGTCCTTCA GCAACCTTAG GAACCGAGGA
    TCATCCCTCT GCGACATAGC CATCCTGGTG GTGGACATTA TGCACGGCTT GGAGCCACAA
    ACCATCGAGT CCATTCAGCT GCTCAAGAAG AAGAAATGTC CGTTCATTGT GGCCCTGAAC
    AAGATCGATC GTTTGTACGA TTGGAAGCAG TTGGCCAGAC GGGATGTGAG GGATGTTCTC
    AAGGAGCAGC AGAGCAACAC ACAGCTGGAG TTTCAGCAGC GCACAAAGGA CGTGATCCTG
    CAGTTCGCCG AGCAGGGCTT GAATGCTGCT CTATTCTACG AAAACACCGA TCCGAAGACA
    TACATTTCCC TGGTGCCCAC CAGTGCCATT TCGGGCGAGG GTATGGGCAA CCTGCTCTTT
    ATGATCGCCG ACTTCTGTCA GAATATGCTG GCCAAGCGCC TGATGTACTC CGAGGAACTG
    CAGGCCACCG TGTTGGAGGT GAAGGCGCTG CCAGGTCTTG GAACCACCAT CGATGCCATT
    CTGATCAATG GAAAGCTCCG CGAGGGTCAG ACCATGGTTG TGGCCGGCAC TGATGGTCCC
    ATAGTCACCC AGATCCGATC CCTGCTGATG CCGCAGCCCA TGAAGGAGCT GCGTGTGAAG
    AACGCCTATG TGGAGTACAA GGAGGTTAAG GCTGCGCAGG GCGTTAAAAT CGCCGCCAAG
    GATCTGGAGA AGGCTATTGC GGGCATCAAT CTGCTGATTG CCCATAAGCC CGATGAAGTC
    GAGATTTGCA CAGAGGAAGT TGCCCGGGAG CTCAAGAGTG CCCTTAGTCA TATTAAACTG
    GCTCAGACAG GCGTTCATGT CCAGGCCTCC ACGCTGGGTT CGTTGGAGGC TCTTCTGGAG
    TTCTTGCGCA CATCTAAGAT TCCTTACTCT GCTATACGAA TTGGACCCGT GGTGAAACGC
    GATGTGATGA AGGCCTCCAC AATGTTGGAA CACGAGGCGC AGTATGCAAC GATTCTGGCA
    TTCGACGTGA AGATCGAGCG GGAGGCACAG GAAATGGCCG ATTCCCTGGG CGTAAAAATA
    TTCCAAGCGG ACATTATCTA CCATCTCTTC GACAAGTTCA CCGCCTATCG CGAGGAGCTG
    AAGCAGAAGA AGCGCGAGGA GTTCCGATCC GTAGCCGTCT TTCCCTGCAA GTTAAGGATA
    CTGCCACAGT TCGTATTCAA CAGCCGAGAT CCGATCGTGA TGGGCGTGAT GGTCGAGAAC
    GGCATTGTCA AAGTGGGCAC TCCAATTTGC GTGCCCAGCA AGGAGTTCGT GGACATTGGA
    ATCGTGACTT CCATCGAATC GAATCACAAG CAAATCGAAT TCGCGCGCAA GGGTCAAGAG
    ATTTGCGTGA AAATCGATCC GATTCCCGGT GAATCGCCCA AAATGTTTGG TCGCCACTTC
    GAGGCCGATG ATATGCTGAT TAGCAAGATC TCTCGCCAGA GCATTGATGC CTGCAAGGAT
    TACTTCCGTG ACGATCTGAT CAAGGCGGAT TGGGCGCTGA TGGTGGAGCT GAAGAAGCTC
    TTCGAGATTC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.