Afti cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Afti
Entrez Gene ID	41744
Full Name	Aftiphilin
Synonyms	CG8538,Dmel\CG8538,dAfti
General protein information	Preferred Names Aftiphilin Names CG8538 gene product from transcript CG8538-RD Afti-PB Afti-PC Afti-PD CG8538-PB CG8538-PC CG8538-PD aftiphilin dAftiphilin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88B3-88B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260173.2	NP_001247102.1	aftiphilin, isoform D
NM_001370051.1	NP_001356916.1	aftiphilin, isoform E
NM_001260172.2	NP_001247101.1	aftiphilin, isoform C
NM_142098.4	NP_650355.2	aftiphilin, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CG8538p, an uncharacterized Drosophila protein, shares significant structural and functional characteristics with Aftiphilin, a mammalian AP-1 accessory protein.
Title: AP-1 clathrin adaptor and CG8538/Aftiphilin are involved in Notch signaling during eye development in Drosophila melanogaster.

The following Afti gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Afti cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa87691	NM_001370051.1 Latest version!	Drosophila melanogaster aftiphilin, transcript variant E (Afti), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OFa04861	NM_001260172.2 Latest version!	Drosophila melanogaster aftiphilin, transcript variant C (Afti), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa04861	NM_001260173.2 Latest version!	Drosophila melanogaster aftiphilin, transcript variant D (Afti), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa04861	NM_142098.4 Latest version!	Drosophila melanogaster aftiphilin, transcript variant B (Afti), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Afti ( NM_001370051.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001356916.1 Drosophila melanogaster aftiphilin, transcript variant E (Afti), mRNA.

Price & Availability↑

CloneID	OFa87691
Clone ID Related Accession (Same CDS sequence)	NM_001370051.1
Accession Version	NM_001370051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	aftiphilin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGAATG TACCACCGCT GCTCTGCAGC ACCCCCCCGC CCATCGATTT TGGTGAGGAA 
GACGACGATT CCGGTTTACA ATCCACCATC CATTTGGACG AGGGTGACGA ATACTCAGAT 
TTTGGCTTAG TCAGCAGCTC GAAAGAACCC CCTCCACCAC CAGATCCCGT GGAGGAATTT 
CGAGAAAAGC CACCTGATTT GCTGGAAGAC AAACAAACCG TCGAGGCATT TAATTACCAG 
GTGAAACAGA AGATGGATTA CGATGTTTTC GAGGAAACAG CACCGCCGAC GCCGCCTCCG 
TTGAGCTTGG AACTGGATGA TGAGGAACTG GAGGAGCATG TGCCCTCCCT TAAGTTGGAC 
AGTCTAAGTC TCTATTCCAC GGAAAGCGTT TCTGCCGCCT CCACACTCTC ACCGGTTGCG 
GAGACTAGTG CAGTTGCTCC GCCGGCTATG CAAACAACCA AAGCCCATAT CCTCAGCCAC 
CAAGTCACGC TGGAGGATGT GTCCGATGAC AGCGACGAGG AGTGCTCGCC AAAGAAGCCC 
AAAGAACTAT TTATACGCGA GGGAGCTGTG GATTTCTTTG CCATAGAGGT ATTGGCCACT 
CCAACCCTAC CATATGGAGA AGGCTTTTCG GAGGAGAATG TGGATACAGG GAAAGGAGAA 
TACGTAGAGA ATACCTCCCA GCCAGAAGAA AGCTTTAGAA GTTTGGAAGC CCAGGAGGAA 
AGTTCAAATA CCTTAGAAAA GCCTATGGAA AATCAAAGCC GTGGCTTATC AAATCCTCCA 
AATCAGGAGG AATCCCCTCA CCAATGGGGT GAACCAGCAA ATAGTGATAC ATATTCATTT 
ACAAATCTTG ATGCGACGCC AAAAGACTTA GTACACAAAG AAATTGTTCC CGAAATGCAA 
GAGCAAGAAG ATGATGATGA CTTCGGTGAC TTTGCGGATT TCTCAGCAGC AGAACCCCAA 
CTAGAAATCA CGGAGAATTC AACGTCTGCC TTTGAGACAC CTTCCTTAGC AATCAATTCC 
CGAACGGAAT CTCAACCTCT AGAGGTTAAG ACGGCTCCTA GCACAGAAGA ATCCTCTAAG 
TTACCTCCTG CAGAGACCGA TGATGGCTTC GATGATTTTC AGGAGTTTGC CACTCCTGCG 
AATGGAAACC ACGGTCAAAG CGAAGACGAC GATGATTTTG GCGACTTCGC CGCGCCCGTA 
GTGACTCCTG TTTCTGTACC TCCGCCACCG CCGCCAGCTG CTGTCCATCT GAGCATAGAT 
GAAAGGGTTA AGCCAGTCCT TGATATGATG TTTCCACAAC CAACGGAACT GGACAGCAAA 
AGTATGGACA ATCGCAGGCC TTTGGCTCAG TGCCAGCGAT TCAATTTTGG TGCCATAGAG 
CATGCCCAAG CCCTTGATTA CCAATGGAGC AGCTCGGAAA TGAGACACGC CCTTGTTCGA 
TCGCTGGGCA TCGATTCAAG GAATATACTC TTCGGCGACA AGTGGAACTC ATCGATGCCA 
CGTTTTGCTG CCAACCTGAG CTTTGATCCG CTGAAGCCGC TGAAACCTCA ATCAACCGGC 
GCCGCAAGCA GCCTGGAGCC CATTTCCAAT TCCACCAGCT ACCAGGAATC ACATGTACAG 
ACATTACAAT TGGAACAACT AGACCAACTA ACGGTGGTGG CTAATGCTGA TAAAATAAAT 
CTTGTTACCT CTAACTCCCA CACCAATAAC AACAACGATA ATGGTGATAA CGACGTTGCT 
GCACCCGTTG ATCAACTGGA TTGTATGCCA CCTGTGATGG ATTTGATGAC CCATAACGAT 
CACAATTTGA CAATCGATTT GCACCACTCT CCATCACTCA CACATTTTGC CACCATCTGC 
AACAACAATA ATAACGATAA TAACAATCCC AACAATCTTA ACAACAATCA TGCAAAAAAT 
ACTGCAACAA TGACAATAAC AATTAACACA AACCTCACTG CTCACGACGC CTTGCTGGAT 
TTTAGATTGG AAAACGCCGC CGCAACAACG GCGAGCCAAA CTGAAACGCG GACTGGCCCG 
TCTCATGGGG AAGGCTCCAC CGGCTCCTGT TCCGCCAGCA ATTCCGACGG CGAAGAGCGA 
CCGAGTCATA GCCACCAGCC AACTGCAGTC CCCAGCCCCG AATCGAACCA TATTGAATCC 
ACAGCAGCGG CATCAGCAGC AGCTGTTGCT GCAGCAGCAG CGACGCTACC GACTACTCTA 
TCAGCAGACA GCCCTGCCCA CAAACTCAAC GGCGGTGAGC AGCTGCATCA GCAGCGGTCG 
GAGCCGGAGC AGTTTGATTT GAGGACCACT CCTCCGGCCG CAGCGCCAGC TCAGGAGGAG 
ATCGTGGGCA GCTCGATGGC CAGCTATTCG GTGCCGCTGA AGGAGACCCA TATCTATACG 
CCCTCCAAAT CGGATACGGC GGTGGCCAAG ACCACCACAC TAGCGCCCAT TGATTTCGAC 
TACGAGATGG CTGCGACGGG CATTATCATC GATGAGACGG TGGTGAAGAA GGAGTATCGC 
GACGTGGAGT ACAAACCCCC ATTTGGTCTG GATTCGCCCA GCAAACTGAG TGGCAATGGA 
GCTGCCAGCA GCTTTCAGTT GCCACCACAG ACGACGGAAG ATGACGACTT TAGTGATTTT 
CAATCTATGC CGGCGCCAAG GTCTCGAATT GATACACCCA CTTTTGGCGA ACAGATGATC 
CTTAGTCCGG CTATTCTGCT GCCCCAAGCC ATTCCGCTGG CAAAGAAGCC AGCTGCAACT 
ATTGAATGGG GCGACAATGC TCTGTCTAGC ATAAATGCCG AGGAAATGGC CAGGATAGAG 
GAGTTGTTCT CCTCGCAGGC CAAACCGCTC ACCATAAGCG CTTCGGTGGC CAAGAAACCA 
GCTCCTTCCA CGCCAGCTCA GTCTCAGCCT CAAGAAGACG ATGAATGGTC GGACTTTGTC 
TCTGTTCCAG TCACCCAACA GCAACCGCAT CATCAGCAAA ACGCAATTGC TAACAACAAT 
AATATAGTCA ACAGCAACGC TAGGAAACCA GCTGCTCCCA AAGAAGATGA TTGGTCGGAT 
TTTGTGAGCA GTACTCCGCC AGCACGACCG GCTCCACAAT TCAATTCCGG TGCGTGGCAG 
AGCGCTAATT TCTACAACAA TCCCTTGAGT TTGTACCAGG CGCAGCAGCA GCTGCAGCAA 
CAGCCTCATA GCAATCTAGT GCCCAAGGGC AGCACCAGCA GCAGCAATAA CAACAATGTG 
CCAGCCATTC CACAGCAAAT ACACATCATG CACGACTTCT CGACTGCACC CGTGTCTGGA 
GGATTGGGTG TGGGTGCCAC CTACCAGCAG CAGCATCAGC GGCAGCAGTT CCAAATCGGC 
AACGCCAAGG TGGCTCCACG CATCTCCCTG ATCCCCGATC TCAGCTTCGT TGCACCGGCC 
TTGCCCACAA ATGCTGGTGC GTTCATGGGT GCGCTACCGA AACCCACTTT CGGTGGCAAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356916.1
CDS	409..3894
Translation	MVNVPPLLCSTPPPIDFGEEDDDSGLQSTIHLDEGDEYSDFGLVSSSKEPPPPPDPVEEFREKPPDLLEDKQTVEAFNYQVKQKMDYDVFEETAPPTPPPLSLELDDEELEEHVPSLKLDSLSLYSTESVSAASTLSPVAETSAVAPPAMQTTKAHILSHQVTLEDVSDDSDEECSPKKPKELFIREGAVDFFAIEVLATPTLPYGEGFSEENVDTGKGEYVENTSQPEESFRSLEAQEESSNTLEKPMENQSRGLSNPPNQEESPHQWGEPANSDTYSFTNLDATPKDLVHKEIVPEMQEQEDDDDFGDFADFSAAEPQLEITENSTSAFETPSLAINSRTESQPLEVKTAPSTEESSKLPPAETDDGFDDFQEFATPANGNHGQSEDDDDFGDFAAPVVTPVSVPPPPPPAAVHLSIDERVKPVLDMMFPQPTELDSKSMDNRRPLAQCQRFNFGAIEHAQALDYQWSSSEMRHALVRSLGIDSRNILFGDKWNSSMPRFAANLSFDPLKPLKPQSTGAASSLEPISNSTSYQESHVQTLQLEQLDQLTVVANADKINLVTSNSHTNNNNDNGDNDVAAPVDQLDCMPPVMDLMTHNDHNLTIDLHHSPSLTHFATICNNNNNDNNNPNNLNNNHAKNTATMTITINTNLTAHDALLDFRLENAAATTASQTETRTGPSHGEGSTGSCSASNSDGEERPSHSHQPTAVPSPESNHIESTAAASAAAVAAAAATLPTTLSADSPAHKLNGGEQLHQQRSEPEQFDLRTTPPAAAPAQEEIVGSSMASYSVPLKETHIYTPSKSDTAVAKTTTLAPIDFDYEMAATGIIIDETVVKKEYRDVEYKPPFGLDSPSKLSGNGAASSFQLPPQTTEDDDFSDFQSMPAPRSRIDTPTFGEQMILSPAILLPQAIPLAKKPAATIEWGDNALSSINAEEMARIEELFSSQAKPLTISASVAKKPAPSTPAQSQPQEDDEWSDFVSVPVTQQQPHHQQNAIANNNNIVNSNARKPAAPKEDDWSDFVSSTPPARPAPQFNSGAWQSANFYNNPLSLYQAQQQLQQQPHSNLVPKGSTSSSNNNNVPAIPQQIHIMHDFSTAPVSGGLGVGATYQQQHQRQQFQIGNAKVAPRISLIPDLSFVAPALPTNAGAFMGALPKPTFGGKK

Target ORF information:

RefSeq Version	NM_001370051.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster aftiphilin, transcript variant E (Afti), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370051.1

ORF Insert Sequence:

ATGGTGAATG TACCACCGCT GCTCTGCAGC ACCCCCCCGC CCATCGATTT TGGTGAGGAA 
GACGACGATT CCGGTTTACA ATCCACCATC CATTTGGACG AGGGTGACGA ATACTCAGAT 
TTTGGCTTAG TCAGCAGCTC GAAAGAACCC CCTCCACCAC CAGATCCCGT GGAGGAATTT 
CGAGAAAAGC CACCTGATTT GCTGGAAGAC AAACAAACCG TCGAGGCATT TAATTACCAG 
GTGAAACAGA AGATGGATTA CGATGTTTTC GAGGAAACAG CACCGCCGAC GCCGCCTCCG 
TTGAGCTTGG AACTGGATGA TGAGGAACTG GAGGAGCATG TGCCCTCCCT TAAGTTGGAC 
AGTCTAAGTC TCTATTCCAC GGAAAGCGTT TCTGCCGCCT CCACACTCTC ACCGGTTGCG 
GAGACTAGTG CAGTTGCTCC GCCGGCTATG CAAACAACCA AAGCCCATAT CCTCAGCCAC 
CAAGTCACGC TGGAGGATGT GTCCGATGAC AGCGACGAGG AGTGCTCGCC AAAGAAGCCC 
AAAGAACTAT TTATACGCGA GGGAGCTGTG GATTTCTTTG CCATAGAGGT ATTGGCCACT 
CCAACCCTAC CATATGGAGA AGGCTTTTCG GAGGAGAATG TGGATACAGG GAAAGGAGAA 
TACGTAGAGA ATACCTCCCA GCCAGAAGAA AGCTTTAGAA GTTTGGAAGC CCAGGAGGAA 
AGTTCAAATA CCTTAGAAAA GCCTATGGAA AATCAAAGCC GTGGCTTATC AAATCCTCCA 
AATCAGGAGG AATCCCCTCA CCAATGGGGT GAACCAGCAA ATAGTGATAC ATATTCATTT 
ACAAATCTTG ATGCGACGCC AAAAGACTTA GTACACAAAG AAATTGTTCC CGAAATGCAA 
GAGCAAGAAG ATGATGATGA CTTCGGTGAC TTTGCGGATT TCTCAGCAGC AGAACCCCAA 
CTAGAAATCA CGGAGAATTC AACGTCTGCC TTTGAGACAC CTTCCTTAGC AATCAATTCC 
CGAACGGAAT CTCAACCTCT AGAGGTTAAG ACGGCTCCTA GCACAGAAGA ATCCTCTAAG 
TTACCTCCTG CAGAGACCGA TGATGGCTTC GATGATTTTC AGGAGTTTGC CACTCCTGCG 
AATGGAAACC ACGGTCAAAG CGAAGACGAC GATGATTTTG GCGACTTCGC CGCGCCCGTA 
GTGACTCCTG TTTCTGTACC TCCGCCACCG CCGCCAGCTG CTGTCCATCT GAGCATAGAT 
GAAAGGGTTA AGCCAGTCCT TGATATGATG TTTCCACAAC CAACGGAACT GGACAGCAAA 
AGTATGGACA ATCGCAGGCC TTTGGCTCAG TGCCAGCGAT TCAATTTTGG TGCCATAGAG 
CATGCCCAAG CCCTTGATTA CCAATGGAGC AGCTCGGAAA TGAGACACGC CCTTGTTCGA 
TCGCTGGGCA TCGATTCAAG GAATATACTC TTCGGCGACA AGTGGAACTC ATCGATGCCA 
CGTTTTGCTG CCAACCTGAG CTTTGATCCG CTGAAGCCGC TGAAACCTCA ATCAACCGGC 
GCCGCAAGCA GCCTGGAGCC CATTTCCAAT TCCACCAGCT ACCAGGAATC ACATGTACAG 
ACATTACAAT TGGAACAACT AGACCAACTA ACGGTGGTGG CTAATGCTGA TAAAATAAAT 
CTTGTTACCT CTAACTCCCA CACCAATAAC AACAACGATA ATGGTGATAA CGACGTTGCT 
GCACCCGTTG ATCAACTGGA TTGTATGCCA CCTGTGATGG ATTTGATGAC CCATAACGAT 
CACAATTTGA CAATCGATTT GCACCACTCT CCATCACTCA CACATTTTGC CACCATCTGC 
AACAACAATA ATAACGATAA TAACAATCCC AACAATCTTA ACAACAATCA TGCAAAAAAT 
ACTGCAACAA TGACAATAAC AATTAACACA AACCTCACTG CTCACGACGC CTTGCTGGAT 
TTTAGATTGG AAAACGCCGC CGCAACAACG GCGAGCCAAA CTGAAACGCG GACTGGCCCG 
TCTCATGGGG AAGGCTCCAC CGGCTCCTGT TCCGCCAGCA ATTCCGACGG CGAAGAGCGA 
CCGAGTCATA GCCACCAGCC AACTGCAGTC CCCAGCCCCG AATCGAACCA TATTGAATCC 
ACAGCAGCGG CATCAGCAGC AGCTGTTGCT GCAGCAGCAG CGACGCTACC GACTACTCTA 
TCAGCAGACA GCCCTGCCCA CAAACTCAAC GGCGGTGAGC AGCTGCATCA GCAGCGGTCG 
GAGCCGGAGC AGTTTGATTT GAGGACCACT CCTCCGGCCG CAGCGCCAGC TCAGGAGGAG 
ATCGTGGGCA GCTCGATGGC CAGCTATTCG GTGCCGCTGA AGGAGACCCA TATCTATACG 
CCCTCCAAAT CGGATACGGC GGTGGCCAAG ACCACCACAC TAGCGCCCAT TGATTTCGAC 
TACGAGATGG CTGCGACGGG CATTATCATC GATGAGACGG TGGTGAAGAA GGAGTATCGC 
GACGTGGAGT ACAAACCCCC ATTTGGTCTG GATTCGCCCA GCAAACTGAG TGGCAATGGA 
GCTGCCAGCA GCTTTCAGTT GCCACCACAG ACGACGGAAG ATGACGACTT TAGTGATTTT 
CAATCTATGC CGGCGCCAAG GTCTCGAATT GATACACCCA CTTTTGGCGA ACAGATGATC 
CTTAGTCCGG CTATTCTGCT GCCCCAAGCC ATTCCGCTGG CAAAGAAGCC AGCTGCAACT 
ATTGAATGGG GCGACAATGC TCTGTCTAGC ATAAATGCCG AGGAAATGGC CAGGATAGAG 
GAGTTGTTCT CCTCGCAGGC CAAACCGCTC ACCATAAGCG CTTCGGTGGC CAAGAAACCA 
GCTCCTTCCA CGCCAGCTCA GTCTCAGCCT CAAGAAGACG ATGAATGGTC GGACTTTGTC 
TCTGTTCCAG TCACCCAACA GCAACCGCAT CATCAGCAAA ACGCAATTGC TAACAACAAT 
AATATAGTCA ACAGCAACGC TAGGAAACCA GCTGCTCCCA AAGAAGATGA TTGGTCGGAT 
TTTGTGAGCA GTACTCCGCC AGCACGACCG GCTCCACAAT TCAATTCCGG TGCGTGGCAG 
AGCGCTAATT TCTACAACAA TCCCTTGAGT TTGTACCAGG CGCAGCAGCA GCTGCAGCAA 
CAGCCTCATA GCAATCTAGT GCCCAAGGGC AGCACCAGCA GCAGCAATAA CAACAATGTG 
CCAGCCATTC CACAGCAAAT ACACATCATG CACGACTTCT CGACTGCACC CGTGTCTGGA 
GGATTGGGTG TGGGTGCCAC CTACCAGCAG CAGCATCAGC GGCAGCAGTT CCAAATCGGC 
AACGCCAAGG TGGCTCCACG CATCTCCCTG ATCCCCGATC TCAGCTTCGT TGCACCGGCC 
TTGCCCACAA ATGCTGGTGC GTTCATGGGT GCGCTACCGA AACCCACTTT CGGTGGCAAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Afti ( NM_001260173.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247102.1 Drosophila melanogaster aftiphilin, transcript variant D (Afti), mRNA.

Price & Availability↑

CloneID	OFa04861
Clone ID Related Accession (Same CDS sequence)	NM_001260173.2 , NM_001260172.2 , NM_142098.4
Accession Version	NM_001260173.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	aftiphilin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260173.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGAATG TACCACCGCT GCTCTGCAGC ACCCCCCCGC CCATCGATTT TGGTGAGGAA 
GACGACGATT CCGGTTTACA ATCCACCATC CATTTGGACG AGGGTGACGA ATACTCAGAT 
TTTGGCTTAG TCAGCAGCTC GAAAGAACCC CCTCCACCAC CAGATCCCGT GGAGGAATTT 
CGAGAAAAGC CACCTGATTT GCTGGAAGAC AAACAAACCG TCGAGGCATT TAATTACCAG 
GTGAAACAGA AGATGGATTA CGATGTTTTC GAGGAAACAG CACCGCCGAC GCCGCCTCCG 
TTGAGCTTGG AACTGGATGA TGAGGAACTG GAGGAGCATG TGCCCTCCCT TAAGTTGGAC 
AGTCTAAGTC TCTATTCCAC GGAAAGCGTT TCTGCCGCCT CCACACTCTC ACCGGTTGCG 
GAGACTAGTG CAGTTGCTCC GCCGGCTATG CAAACAACCA AAGCCCATAT CCTCAGCCAC 
CAAGTCACGC TGGAGGATGT GTCCGATGAC AGCGACGAGG AGTGCTCGCC AAAGAAGCCC 
AAAGAACTAT TTATACGCGA GGGAGCTGTG GATTTCTTTG CCATAGAGGT ATTGGCCACT 
CCAACCCTAC CATATGGAGA AGGCTTTTCG GAGGAGAATG TGGATACAGG GAAAGGAGAA 
TACGTAGAGA ATACCTCCCA GCCAGAAGAA AGCTTTAGAA GTTTGGAAGC CCAGGAGGAA 
AGTTCAAATA CCTTAGAAAA GCCTATGGAA AATCAAAGCC GTGGCTTATC AAATCCTCCA 
AATCAGGAGG AATCCCCTCA CCAATGGGGT GAACCAGCAA ATAGTGATAC ATATTCATTT 
ACAAATCTTG ATGCGACGCC AAAAGACTTA GTACACAAAG AAATTGTTCC CGAAATGCAA 
GAGCAAGAAG ATGATGATGA CTTCGGTGAC TTTGCGGATT TCTCAGCAGC AGAACCCCAA 
CTAGAAATCA CGGAGAATTC AACGTCTGCC TTTGAGACAC CTTCCTTAGC AATCAATTCC 
CGAACGGAAT CTCAACCTCT AGAGGTTAAG ACGGCTCCTA GCACAGAAGA ATCCTCTAAG 
TTACCTCCTG CAGAGACCGA TGATGGCTTC GATGATTTTC AGGAGTTTGC CACTCCTGCG 
AATGGAAACC ACGGTCAAAG CGAAGACGAC GATGATTTTG GCGACTTCGC CGCGCCCGTA 
GTGACTCCTG TTTCTGTACC TCCGCCACCG CCGCCAGCTG CTGTCCATCT GAGCATAGAT 
GAAAGGGTTA AGCCAGTCCT TGATATGATG TTTCCACAAC CAACGGAACT GGACAGCAAA 
AGTATGGACA ATCGCAGGCC TTTGGCTCAG TGCCAGCGAT TCAATTTTGG TGCCATAGAG 
CATGCCCAAG CCCTTGATTA CCAATGGAGC AGCTCGGAAA TGAGACACGC CCTTGTTCGA 
TCGCTGGGCA TCGATTCAAG GAATATACTC TTCGGCGACA AGTGGAACTC ATCGATGCCA 
CGTTTTGCTG CCAACCTGAG CTTTGATCCG CTGAAGCCGC TGAAACCTCA ATCAACCGGC 
GCCGCAAGCA GCCTGGAGCC CATTTCCAAT TCCACCAGCT ACCAGGGATT GGAAAACGCC 
GCCGCAACAA CGGCGAGCCA AACTGAAACG CGGACTGGCC CGTCTCATGG GGAAGGCTCC 
ACCGGCTCCT GTTCCGCCAG CAATTCCGAC GGCGAAGAGC GACCGAGTCA TAGCCACCAG 
CCAACTGCAG TCCCCAGCCC CGAATCGAAC CATATTGAAT CCACAGCAGC GGCATCAGCA 
GCAGCTGTTG CTGCAGCAGC AGCGACGCTA CCGACTACTC TATCAGCAGA CAGCCCTGCC 
CACAAACTCA ACGGCGGTGA GCAGCTGCAT CAGCAGCGGT CGGAGCCGGA GCAGTTTGAT 
TTGAGGACCA CTCCTCCGGC CGCAGCGCCA GCTCAGGAGG AGATCGTGGG CAGCTCGATG 
GCCAGCTATT CGGTGCCGCT GAAGGAGACC CATATCTATA CGCCCTCCAA ATCGGATACG 
GCGGTGGCCA AGACCACCAC ACTAGCGCCC ATTGATTTCG ACTACGAGAT GGCTGCGACG 
GGCATTATCA TCGATGAGAC GGTGGTGAAG AAGGAGTATC GCGACGTGGA GTACAAACCC 
CCATTTGGTC TGGATTCGCC CAGCAAACTG AGTGGCAATG GAGCTGCCAG CAGCTTTCAG 
TTGCCACCAC AGACGACGGA AGATGACGAC TTTAGTGATT TTCAATCTAT GCCGGCGCCA 
AGGTCTCGAA TTGATACACC CACTTTTGGC GAACAGATGA TCCTTAGTCC GGCTATTCTG 
CTGCCCCAAG CCATTCCGCT GGCAAAGAAG CCAGCTGCAA CTATTGAATG GGGCGACAAT 
GCTCTGTCTA GCATAAATGC CGAGGAAATG GCCAGGATAG AGGAGTTGTT CTCCTCGCAG 
GCCAAACCGC TCACCATAAG CGCTTCGGTG GCCAAGAAAC CAGCTCCTTC CACGCCAGCT 
CAGTCTCAGC CTCAAGAAGA CGATGAATGG TCGGACTTTG TCTCTGTTCC AGTCACCCAA 
CAGCAACCGC ATCATCAGCA AAACGCAATT GCTAACAACA ATAATATAGT CAACAGCAAC 
GCTAGGAAAC CAGCTGCTCC CAAAGAAGAT GATTGGTCGG ATTTTGTGAG CAGTACTCCG 
CCAGCACGAC CGGCTCCACA ATTCAATTCC GGTGCGTGGC AGAGCGCTAA TTTCTACAAC 
AATCCCTTGA GTTTGTACCA GGCGCAGCAG CAGCTGCAGC AACAGCCTCA TAGCAATCTA 
GTGCCCAAGG GCAGCACCAG CAGCAGCAAT AACAACAATG TGCCAGCCAT TCCACAGCAA 
ATACACATCA TGCACGACTT CTCGACTGCA CCCGTGTCTG GAGGATTGGG TGTGGGTGCC 
ACCTACCAGC AGCAGCATCA GCGGCAGCAG TTCCAAATCG GCAACGCCAA GGTGGCTCCA 
CGCATCTCCC TGATCCCCGA TCTCAGCTTC GTTGCACCGG CCTTGCCCAC AAATGCTGGT 
GCGTTCATGG GTGCGCTACC GAAACCCACT TTCGGTGGCA AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247102.1
CDS	409..3516
Translation	MVNVPPLLCSTPPPIDFGEEDDDSGLQSTIHLDEGDEYSDFGLVSSSKEPPPPPDPVEEFREKPPDLLEDKQTVEAFNYQVKQKMDYDVFEETAPPTPPPLSLELDDEELEEHVPSLKLDSLSLYSTESVSAASTLSPVAETSAVAPPAMQTTKAHILSHQVTLEDVSDDSDEECSPKKPKELFIREGAVDFFAIEVLATPTLPYGEGFSEENVDTGKGEYVENTSQPEESFRSLEAQEESSNTLEKPMENQSRGLSNPPNQEESPHQWGEPANSDTYSFTNLDATPKDLVHKEIVPEMQEQEDDDDFGDFADFSAAEPQLEITENSTSAFETPSLAINSRTESQPLEVKTAPSTEESSKLPPAETDDGFDDFQEFATPANGNHGQSEDDDDFGDFAAPVVTPVSVPPPPPPAAVHLSIDERVKPVLDMMFPQPTELDSKSMDNRRPLAQCQRFNFGAIEHAQALDYQWSSSEMRHALVRSLGIDSRNILFGDKWNSSMPRFAANLSFDPLKPLKPQSTGAASSLEPISNSTSYQGLENAAATTASQTETRTGPSHGEGSTGSCSASNSDGEERPSHSHQPTAVPSPESNHIESTAAASAAAVAAAAATLPTTLSADSPAHKLNGGEQLHQQRSEPEQFDLRTTPPAAAPAQEEIVGSSMASYSVPLKETHIYTPSKSDTAVAKTTTLAPIDFDYEMAATGIIIDETVVKKEYRDVEYKPPFGLDSPSKLSGNGAASSFQLPPQTTEDDDFSDFQSMPAPRSRIDTPTFGEQMILSPAILLPQAIPLAKKPAATIEWGDNALSSINAEEMARIEELFSSQAKPLTISASVAKKPAPSTPAQSQPQEDDEWSDFVSVPVTQQQPHHQQNAIANNNNIVNSNARKPAAPKEDDWSDFVSSTPPARPAPQFNSGAWQSANFYNNPLSLYQAQQQLQQQPHSNLVPKGSTSSSNNNNVPAIPQQIHIMHDFSTAPVSGGLGVGATYQQQHQRQQFQIGNAKVAPRISLIPDLSFVAPALPTNAGAFMGALPKPTFGGKK

Target ORF information:

RefSeq Version	NM_001260173.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster aftiphilin, transcript variant D (Afti), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260173.2

ORF Insert Sequence:

ATGGTGAATG TACCACCGCT GCTCTGCAGC ACCCCCCCGC CCATCGATTT TGGTGAGGAA 
GACGACGATT CCGGTTTACA ATCCACCATC CATTTGGACG AGGGTGACGA ATACTCAGAT 
TTTGGCTTAG TCAGCAGCTC GAAAGAACCC CCTCCACCAC CAGATCCCGT GGAGGAATTT 
CGAGAAAAGC CACCTGATTT GCTGGAAGAC AAACAAACCG TCGAGGCATT TAATTACCAG 
GTGAAACAGA AGATGGATTA CGATGTTTTC GAGGAAACAG CACCGCCGAC GCCGCCTCCG 
TTGAGCTTGG AACTGGATGA TGAGGAACTG GAGGAGCATG TGCCCTCCCT TAAGTTGGAC 
AGTCTAAGTC TCTATTCCAC GGAAAGCGTT TCTGCCGCCT CCACACTCTC ACCGGTTGCG 
GAGACTAGTG CAGTTGCTCC GCCGGCTATG CAAACAACCA AAGCCCATAT CCTCAGCCAC 
CAAGTCACGC TGGAGGATGT GTCCGATGAC AGCGACGAGG AGTGCTCGCC AAAGAAGCCC 
AAAGAACTAT TTATACGCGA GGGAGCTGTG GATTTCTTTG CCATAGAGGT ATTGGCCACT 
CCAACCCTAC CATATGGAGA AGGCTTTTCG GAGGAGAATG TGGATACAGG GAAAGGAGAA 
TACGTAGAGA ATACCTCCCA GCCAGAAGAA AGCTTTAGAA GTTTGGAAGC CCAGGAGGAA 
AGTTCAAATA CCTTAGAAAA GCCTATGGAA AATCAAAGCC GTGGCTTATC AAATCCTCCA 
AATCAGGAGG AATCCCCTCA CCAATGGGGT GAACCAGCAA ATAGTGATAC ATATTCATTT 
ACAAATCTTG ATGCGACGCC AAAAGACTTA GTACACAAAG AAATTGTTCC CGAAATGCAA 
GAGCAAGAAG ATGATGATGA CTTCGGTGAC TTTGCGGATT TCTCAGCAGC AGAACCCCAA 
CTAGAAATCA CGGAGAATTC AACGTCTGCC TTTGAGACAC CTTCCTTAGC AATCAATTCC 
CGAACGGAAT CTCAACCTCT AGAGGTTAAG ACGGCTCCTA GCACAGAAGA ATCCTCTAAG 
TTACCTCCTG CAGAGACCGA TGATGGCTTC GATGATTTTC AGGAGTTTGC CACTCCTGCG 
AATGGAAACC ACGGTCAAAG CGAAGACGAC GATGATTTTG GCGACTTCGC CGCGCCCGTA 
GTGACTCCTG TTTCTGTACC TCCGCCACCG CCGCCAGCTG CTGTCCATCT GAGCATAGAT 
GAAAGGGTTA AGCCAGTCCT TGATATGATG TTTCCACAAC CAACGGAACT GGACAGCAAA 
AGTATGGACA ATCGCAGGCC TTTGGCTCAG TGCCAGCGAT TCAATTTTGG TGCCATAGAG 
CATGCCCAAG CCCTTGATTA CCAATGGAGC AGCTCGGAAA TGAGACACGC CCTTGTTCGA 
TCGCTGGGCA TCGATTCAAG GAATATACTC TTCGGCGACA AGTGGAACTC ATCGATGCCA 
CGTTTTGCTG CCAACCTGAG CTTTGATCCG CTGAAGCCGC TGAAACCTCA ATCAACCGGC 
GCCGCAAGCA GCCTGGAGCC CATTTCCAAT TCCACCAGCT ACCAGGGATT GGAAAACGCC 
GCCGCAACAA CGGCGAGCCA AACTGAAACG CGGACTGGCC CGTCTCATGG GGAAGGCTCC 
ACCGGCTCCT GTTCCGCCAG CAATTCCGAC GGCGAAGAGC GACCGAGTCA TAGCCACCAG 
CCAACTGCAG TCCCCAGCCC CGAATCGAAC CATATTGAAT CCACAGCAGC GGCATCAGCA 
GCAGCTGTTG CTGCAGCAGC AGCGACGCTA CCGACTACTC TATCAGCAGA CAGCCCTGCC 
CACAAACTCA ACGGCGGTGA GCAGCTGCAT CAGCAGCGGT CGGAGCCGGA GCAGTTTGAT 
TTGAGGACCA CTCCTCCGGC CGCAGCGCCA GCTCAGGAGG AGATCGTGGG CAGCTCGATG 
GCCAGCTATT CGGTGCCGCT GAAGGAGACC CATATCTATA CGCCCTCCAA ATCGGATACG 
GCGGTGGCCA AGACCACCAC ACTAGCGCCC ATTGATTTCG ACTACGAGAT GGCTGCGACG 
GGCATTATCA TCGATGAGAC GGTGGTGAAG AAGGAGTATC GCGACGTGGA GTACAAACCC 
CCATTTGGTC TGGATTCGCC CAGCAAACTG AGTGGCAATG GAGCTGCCAG CAGCTTTCAG 
TTGCCACCAC AGACGACGGA AGATGACGAC TTTAGTGATT TTCAATCTAT GCCGGCGCCA 
AGGTCTCGAA TTGATACACC CACTTTTGGC GAACAGATGA TCCTTAGTCC GGCTATTCTG 
CTGCCCCAAG CCATTCCGCT GGCAAAGAAG CCAGCTGCAA CTATTGAATG GGGCGACAAT 
GCTCTGTCTA GCATAAATGC CGAGGAAATG GCCAGGATAG AGGAGTTGTT CTCCTCGCAG 
GCCAAACCGC TCACCATAAG CGCTTCGGTG GCCAAGAAAC CAGCTCCTTC CACGCCAGCT 
CAGTCTCAGC CTCAAGAAGA CGATGAATGG TCGGACTTTG TCTCTGTTCC AGTCACCCAA 
CAGCAACCGC ATCATCAGCA AAACGCAATT GCTAACAACA ATAATATAGT CAACAGCAAC 
GCTAGGAAAC CAGCTGCTCC CAAAGAAGAT GATTGGTCGG ATTTTGTGAG CAGTACTCCG 
CCAGCACGAC CGGCTCCACA ATTCAATTCC GGTGCGTGGC AGAGCGCTAA TTTCTACAAC 
AATCCCTTGA GTTTGTACCA GGCGCAGCAG CAGCTGCAGC AACAGCCTCA TAGCAATCTA 
GTGCCCAAGG GCAGCACCAG CAGCAGCAAT AACAACAATG TGCCAGCCAT TCCACAGCAA 
ATACACATCA TGCACGACTT CTCGACTGCA CCCGTGTCTG GAGGATTGGG TGTGGGTGCC 
ACCTACCAGC AGCAGCATCA GCGGCAGCAG TTCCAAATCG GCAACGCCAA GGTGGCTCCA 
CGCATCTCCC TGATCCCCGA TCTCAGCTTC GTTGCACCGG CCTTGCCCAC AAATGCTGGT 
GCGTTCATGG GTGCGCTACC GAAACCCACT TTCGGTGGCA AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Afti ( NM_001260172.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247101.1 Drosophila melanogaster aftiphilin, transcript variant C (Afti), mRNA.

Price & Availability↑

CloneID	OFa04861
Clone ID Related Accession (Same CDS sequence)	NM_001260173.2 , NM_001260172.2 , NM_142098.4
Accession Version	NM_001260172.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	aftiphilin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260172.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGAATG TACCACCGCT GCTCTGCAGC ACCCCCCCGC CCATCGATTT TGGTGAGGAA 
GACGACGATT CCGGTTTACA ATCCACCATC CATTTGGACG AGGGTGACGA ATACTCAGAT 
TTTGGCTTAG TCAGCAGCTC GAAAGAACCC CCTCCACCAC CAGATCCCGT GGAGGAATTT 
CGAGAAAAGC CACCTGATTT GCTGGAAGAC AAACAAACCG TCGAGGCATT TAATTACCAG 
GTGAAACAGA AGATGGATTA CGATGTTTTC GAGGAAACAG CACCGCCGAC GCCGCCTCCG 
TTGAGCTTGG AACTGGATGA TGAGGAACTG GAGGAGCATG TGCCCTCCCT TAAGTTGGAC 
AGTCTAAGTC TCTATTCCAC GGAAAGCGTT TCTGCCGCCT CCACACTCTC ACCGGTTGCG 
GAGACTAGTG CAGTTGCTCC GCCGGCTATG CAAACAACCA AAGCCCATAT CCTCAGCCAC 
CAAGTCACGC TGGAGGATGT GTCCGATGAC AGCGACGAGG AGTGCTCGCC AAAGAAGCCC 
AAAGAACTAT TTATACGCGA GGGAGCTGTG GATTTCTTTG CCATAGAGGT ATTGGCCACT 
CCAACCCTAC CATATGGAGA AGGCTTTTCG GAGGAGAATG TGGATACAGG GAAAGGAGAA 
TACGTAGAGA ATACCTCCCA GCCAGAAGAA AGCTTTAGAA GTTTGGAAGC CCAGGAGGAA 
AGTTCAAATA CCTTAGAAAA GCCTATGGAA AATCAAAGCC GTGGCTTATC AAATCCTCCA 
AATCAGGAGG AATCCCCTCA CCAATGGGGT GAACCAGCAA ATAGTGATAC ATATTCATTT 
ACAAATCTTG ATGCGACGCC AAAAGACTTA GTACACAAAG AAATTGTTCC CGAAATGCAA 
GAGCAAGAAG ATGATGATGA CTTCGGTGAC TTTGCGGATT TCTCAGCAGC AGAACCCCAA 
CTAGAAATCA CGGAGAATTC AACGTCTGCC TTTGAGACAC CTTCCTTAGC AATCAATTCC 
CGAACGGAAT CTCAACCTCT AGAGGTTAAG ACGGCTCCTA GCACAGAAGA ATCCTCTAAG 
TTACCTCCTG CAGAGACCGA TGATGGCTTC GATGATTTTC AGGAGTTTGC CACTCCTGCG 
AATGGAAACC ACGGTCAAAG CGAAGACGAC GATGATTTTG GCGACTTCGC CGCGCCCGTA 
GTGACTCCTG TTTCTGTACC TCCGCCACCG CCGCCAGCTG CTGTCCATCT GAGCATAGAT 
GAAAGGGTTA AGCCAGTCCT TGATATGATG TTTCCACAAC CAACGGAACT GGACAGCAAA 
AGTATGGACA ATCGCAGGCC TTTGGCTCAG TGCCAGCGAT TCAATTTTGG TGCCATAGAG 
CATGCCCAAG CCCTTGATTA CCAATGGAGC AGCTCGGAAA TGAGACACGC CCTTGTTCGA 
TCGCTGGGCA TCGATTCAAG GAATATACTC TTCGGCGACA AGTGGAACTC ATCGATGCCA 
CGTTTTGCTG CCAACCTGAG CTTTGATCCG CTGAAGCCGC TGAAACCTCA ATCAACCGGC 
GCCGCAAGCA GCCTGGAGCC CATTTCCAAT TCCACCAGCT ACCAGGGATT GGAAAACGCC 
GCCGCAACAA CGGCGAGCCA AACTGAAACG CGGACTGGCC CGTCTCATGG GGAAGGCTCC 
ACCGGCTCCT GTTCCGCCAG CAATTCCGAC GGCGAAGAGC GACCGAGTCA TAGCCACCAG 
CCAACTGCAG TCCCCAGCCC CGAATCGAAC CATATTGAAT CCACAGCAGC GGCATCAGCA 
GCAGCTGTTG CTGCAGCAGC AGCGACGCTA CCGACTACTC TATCAGCAGA CAGCCCTGCC 
CACAAACTCA ACGGCGGTGA GCAGCTGCAT CAGCAGCGGT CGGAGCCGGA GCAGTTTGAT 
TTGAGGACCA CTCCTCCGGC CGCAGCGCCA GCTCAGGAGG AGATCGTGGG CAGCTCGATG 
GCCAGCTATT CGGTGCCGCT GAAGGAGACC CATATCTATA CGCCCTCCAA ATCGGATACG 
GCGGTGGCCA AGACCACCAC ACTAGCGCCC ATTGATTTCG ACTACGAGAT GGCTGCGACG 
GGCATTATCA TCGATGAGAC GGTGGTGAAG AAGGAGTATC GCGACGTGGA GTACAAACCC 
CCATTTGGTC TGGATTCGCC CAGCAAACTG AGTGGCAATG GAGCTGCCAG CAGCTTTCAG 
TTGCCACCAC AGACGACGGA AGATGACGAC TTTAGTGATT TTCAATCTAT GCCGGCGCCA 
AGGTCTCGAA TTGATACACC CACTTTTGGC GAACAGATGA TCCTTAGTCC GGCTATTCTG 
CTGCCCCAAG CCATTCCGCT GGCAAAGAAG CCAGCTGCAA CTATTGAATG GGGCGACAAT 
GCTCTGTCTA GCATAAATGC CGAGGAAATG GCCAGGATAG AGGAGTTGTT CTCCTCGCAG 
GCCAAACCGC TCACCATAAG CGCTTCGGTG GCCAAGAAAC CAGCTCCTTC CACGCCAGCT 
CAGTCTCAGC CTCAAGAAGA CGATGAATGG TCGGACTTTG TCTCTGTTCC AGTCACCCAA 
CAGCAACCGC ATCATCAGCA AAACGCAATT GCTAACAACA ATAATATAGT CAACAGCAAC 
GCTAGGAAAC CAGCTGCTCC CAAAGAAGAT GATTGGTCGG ATTTTGTGAG CAGTACTCCG 
CCAGCACGAC CGGCTCCACA ATTCAATTCC GGTGCGTGGC AGAGCGCTAA TTTCTACAAC 
AATCCCTTGA GTTTGTACCA GGCGCAGCAG CAGCTGCAGC AACAGCCTCA TAGCAATCTA 
GTGCCCAAGG GCAGCACCAG CAGCAGCAAT AACAACAATG TGCCAGCCAT TCCACAGCAA 
ATACACATCA TGCACGACTT CTCGACTGCA CCCGTGTCTG GAGGATTGGG TGTGGGTGCC 
ACCTACCAGC AGCAGCATCA GCGGCAGCAG TTCCAAATCG GCAACGCCAA GGTGGCTCCA 
CGCATCTCCC TGATCCCCGA TCTCAGCTTC GTTGCACCGG CCTTGCCCAC AAATGCTGGT 
GCGTTCATGG GTGCGCTACC GAAACCCACT TTCGGTGGCA AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247101.1
CDS	409..3516
Translation	MVNVPPLLCSTPPPIDFGEEDDDSGLQSTIHLDEGDEYSDFGLVSSSKEPPPPPDPVEEFREKPPDLLEDKQTVEAFNYQVKQKMDYDVFEETAPPTPPPLSLELDDEELEEHVPSLKLDSLSLYSTESVSAASTLSPVAETSAVAPPAMQTTKAHILSHQVTLEDVSDDSDEECSPKKPKELFIREGAVDFFAIEVLATPTLPYGEGFSEENVDTGKGEYVENTSQPEESFRSLEAQEESSNTLEKPMENQSRGLSNPPNQEESPHQWGEPANSDTYSFTNLDATPKDLVHKEIVPEMQEQEDDDDFGDFADFSAAEPQLEITENSTSAFETPSLAINSRTESQPLEVKTAPSTEESSKLPPAETDDGFDDFQEFATPANGNHGQSEDDDDFGDFAAPVVTPVSVPPPPPPAAVHLSIDERVKPVLDMMFPQPTELDSKSMDNRRPLAQCQRFNFGAIEHAQALDYQWSSSEMRHALVRSLGIDSRNILFGDKWNSSMPRFAANLSFDPLKPLKPQSTGAASSLEPISNSTSYQGLENAAATTASQTETRTGPSHGEGSTGSCSASNSDGEERPSHSHQPTAVPSPESNHIESTAAASAAAVAAAAATLPTTLSADSPAHKLNGGEQLHQQRSEPEQFDLRTTPPAAAPAQEEIVGSSMASYSVPLKETHIYTPSKSDTAVAKTTTLAPIDFDYEMAATGIIIDETVVKKEYRDVEYKPPFGLDSPSKLSGNGAASSFQLPPQTTEDDDFSDFQSMPAPRSRIDTPTFGEQMILSPAILLPQAIPLAKKPAATIEWGDNALSSINAEEMARIEELFSSQAKPLTISASVAKKPAPSTPAQSQPQEDDEWSDFVSVPVTQQQPHHQQNAIANNNNIVNSNARKPAAPKEDDWSDFVSSTPPARPAPQFNSGAWQSANFYNNPLSLYQAQQQLQQQPHSNLVPKGSTSSSNNNNVPAIPQQIHIMHDFSTAPVSGGLGVGATYQQQHQRQQFQIGNAKVAPRISLIPDLSFVAPALPTNAGAFMGALPKPTFGGKK

Target ORF information:

RefSeq Version	NM_001260172.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster aftiphilin, transcript variant C (Afti), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260172.2

ORF Insert Sequence:

ATGGTGAATG TACCACCGCT GCTCTGCAGC ACCCCCCCGC CCATCGATTT TGGTGAGGAA 
GACGACGATT CCGGTTTACA ATCCACCATC CATTTGGACG AGGGTGACGA ATACTCAGAT 
TTTGGCTTAG TCAGCAGCTC GAAAGAACCC CCTCCACCAC CAGATCCCGT GGAGGAATTT 
CGAGAAAAGC CACCTGATTT GCTGGAAGAC AAACAAACCG TCGAGGCATT TAATTACCAG 
GTGAAACAGA AGATGGATTA CGATGTTTTC GAGGAAACAG CACCGCCGAC GCCGCCTCCG 
TTGAGCTTGG AACTGGATGA TGAGGAACTG GAGGAGCATG TGCCCTCCCT TAAGTTGGAC 
AGTCTAAGTC TCTATTCCAC GGAAAGCGTT TCTGCCGCCT CCACACTCTC ACCGGTTGCG 
GAGACTAGTG CAGTTGCTCC GCCGGCTATG CAAACAACCA AAGCCCATAT CCTCAGCCAC 
CAAGTCACGC TGGAGGATGT GTCCGATGAC AGCGACGAGG AGTGCTCGCC AAAGAAGCCC 
AAAGAACTAT TTATACGCGA GGGAGCTGTG GATTTCTTTG CCATAGAGGT ATTGGCCACT 
CCAACCCTAC CATATGGAGA AGGCTTTTCG GAGGAGAATG TGGATACAGG GAAAGGAGAA 
TACGTAGAGA ATACCTCCCA GCCAGAAGAA AGCTTTAGAA GTTTGGAAGC CCAGGAGGAA 
AGTTCAAATA CCTTAGAAAA GCCTATGGAA AATCAAAGCC GTGGCTTATC AAATCCTCCA 
AATCAGGAGG AATCCCCTCA CCAATGGGGT GAACCAGCAA ATAGTGATAC ATATTCATTT 
ACAAATCTTG ATGCGACGCC AAAAGACTTA GTACACAAAG AAATTGTTCC CGAAATGCAA 
GAGCAAGAAG ATGATGATGA CTTCGGTGAC TTTGCGGATT TCTCAGCAGC AGAACCCCAA 
CTAGAAATCA CGGAGAATTC AACGTCTGCC TTTGAGACAC CTTCCTTAGC AATCAATTCC 
CGAACGGAAT CTCAACCTCT AGAGGTTAAG ACGGCTCCTA GCACAGAAGA ATCCTCTAAG 
TTACCTCCTG CAGAGACCGA TGATGGCTTC GATGATTTTC AGGAGTTTGC CACTCCTGCG 
AATGGAAACC ACGGTCAAAG CGAAGACGAC GATGATTTTG GCGACTTCGC CGCGCCCGTA 
GTGACTCCTG TTTCTGTACC TCCGCCACCG CCGCCAGCTG CTGTCCATCT GAGCATAGAT 
GAAAGGGTTA AGCCAGTCCT TGATATGATG TTTCCACAAC CAACGGAACT GGACAGCAAA 
AGTATGGACA ATCGCAGGCC TTTGGCTCAG TGCCAGCGAT TCAATTTTGG TGCCATAGAG 
CATGCCCAAG CCCTTGATTA CCAATGGAGC AGCTCGGAAA TGAGACACGC CCTTGTTCGA 
TCGCTGGGCA TCGATTCAAG GAATATACTC TTCGGCGACA AGTGGAACTC ATCGATGCCA 
CGTTTTGCTG CCAACCTGAG CTTTGATCCG CTGAAGCCGC TGAAACCTCA ATCAACCGGC 
GCCGCAAGCA GCCTGGAGCC CATTTCCAAT TCCACCAGCT ACCAGGGATT GGAAAACGCC 
GCCGCAACAA CGGCGAGCCA AACTGAAACG CGGACTGGCC CGTCTCATGG GGAAGGCTCC 
ACCGGCTCCT GTTCCGCCAG CAATTCCGAC GGCGAAGAGC GACCGAGTCA TAGCCACCAG 
CCAACTGCAG TCCCCAGCCC CGAATCGAAC CATATTGAAT CCACAGCAGC GGCATCAGCA 
GCAGCTGTTG CTGCAGCAGC AGCGACGCTA CCGACTACTC TATCAGCAGA CAGCCCTGCC 
CACAAACTCA ACGGCGGTGA GCAGCTGCAT CAGCAGCGGT CGGAGCCGGA GCAGTTTGAT 
TTGAGGACCA CTCCTCCGGC CGCAGCGCCA GCTCAGGAGG AGATCGTGGG CAGCTCGATG 
GCCAGCTATT CGGTGCCGCT GAAGGAGACC CATATCTATA CGCCCTCCAA ATCGGATACG 
GCGGTGGCCA AGACCACCAC ACTAGCGCCC ATTGATTTCG ACTACGAGAT GGCTGCGACG 
GGCATTATCA TCGATGAGAC GGTGGTGAAG AAGGAGTATC GCGACGTGGA GTACAAACCC 
CCATTTGGTC TGGATTCGCC CAGCAAACTG AGTGGCAATG GAGCTGCCAG CAGCTTTCAG 
TTGCCACCAC AGACGACGGA AGATGACGAC TTTAGTGATT TTCAATCTAT GCCGGCGCCA 
AGGTCTCGAA TTGATACACC CACTTTTGGC GAACAGATGA TCCTTAGTCC GGCTATTCTG 
CTGCCCCAAG CCATTCCGCT GGCAAAGAAG CCAGCTGCAA CTATTGAATG GGGCGACAAT 
GCTCTGTCTA GCATAAATGC CGAGGAAATG GCCAGGATAG AGGAGTTGTT CTCCTCGCAG 
GCCAAACCGC TCACCATAAG CGCTTCGGTG GCCAAGAAAC CAGCTCCTTC CACGCCAGCT 
CAGTCTCAGC CTCAAGAAGA CGATGAATGG TCGGACTTTG TCTCTGTTCC AGTCACCCAA 
CAGCAACCGC ATCATCAGCA AAACGCAATT GCTAACAACA ATAATATAGT CAACAGCAAC 
GCTAGGAAAC CAGCTGCTCC CAAAGAAGAT GATTGGTCGG ATTTTGTGAG CAGTACTCCG 
CCAGCACGAC CGGCTCCACA ATTCAATTCC GGTGCGTGGC AGAGCGCTAA TTTCTACAAC 
AATCCCTTGA GTTTGTACCA GGCGCAGCAG CAGCTGCAGC AACAGCCTCA TAGCAATCTA 
GTGCCCAAGG GCAGCACCAG CAGCAGCAAT AACAACAATG TGCCAGCCAT TCCACAGCAA 
ATACACATCA TGCACGACTT CTCGACTGCA CCCGTGTCTG GAGGATTGGG TGTGGGTGCC 
ACCTACCAGC AGCAGCATCA GCGGCAGCAG TTCCAAATCG GCAACGCCAA GGTGGCTCCA 
CGCATCTCCC TGATCCCCGA TCTCAGCTTC GTTGCACCGG CCTTGCCCAC AAATGCTGGT 
GCGTTCATGG GTGCGCTACC GAAACCCACT TTCGGTGGCA AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Afti ( NM_142098.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650355.2 Drosophila melanogaster aftiphilin, transcript variant B (Afti), mRNA.

Price & Availability↑

CloneID	OFa04861
Clone ID Related Accession (Same CDS sequence)	NM_001260173.2 , NM_001260172.2 , NM_142098.4
Accession Version	NM_142098.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	aftiphilin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142098.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGAATG TACCACCGCT GCTCTGCAGC ACCCCCCCGC CCATCGATTT TGGTGAGGAA 
GACGACGATT CCGGTTTACA ATCCACCATC CATTTGGACG AGGGTGACGA ATACTCAGAT 
TTTGGCTTAG TCAGCAGCTC GAAAGAACCC CCTCCACCAC CAGATCCCGT GGAGGAATTT 
CGAGAAAAGC CACCTGATTT GCTGGAAGAC AAACAAACCG TCGAGGCATT TAATTACCAG 
GTGAAACAGA AGATGGATTA CGATGTTTTC GAGGAAACAG CACCGCCGAC GCCGCCTCCG 
TTGAGCTTGG AACTGGATGA TGAGGAACTG GAGGAGCATG TGCCCTCCCT TAAGTTGGAC 
AGTCTAAGTC TCTATTCCAC GGAAAGCGTT TCTGCCGCCT CCACACTCTC ACCGGTTGCG 
GAGACTAGTG CAGTTGCTCC GCCGGCTATG CAAACAACCA AAGCCCATAT CCTCAGCCAC 
CAAGTCACGC TGGAGGATGT GTCCGATGAC AGCGACGAGG AGTGCTCGCC AAAGAAGCCC 
AAAGAACTAT TTATACGCGA GGGAGCTGTG GATTTCTTTG CCATAGAGGT ATTGGCCACT 
CCAACCCTAC CATATGGAGA AGGCTTTTCG GAGGAGAATG TGGATACAGG GAAAGGAGAA 
TACGTAGAGA ATACCTCCCA GCCAGAAGAA AGCTTTAGAA GTTTGGAAGC CCAGGAGGAA 
AGTTCAAATA CCTTAGAAAA GCCTATGGAA AATCAAAGCC GTGGCTTATC AAATCCTCCA 
AATCAGGAGG AATCCCCTCA CCAATGGGGT GAACCAGCAA ATAGTGATAC ATATTCATTT 
ACAAATCTTG ATGCGACGCC AAAAGACTTA GTACACAAAG AAATTGTTCC CGAAATGCAA 
GAGCAAGAAG ATGATGATGA CTTCGGTGAC TTTGCGGATT TCTCAGCAGC AGAACCCCAA 
CTAGAAATCA CGGAGAATTC AACGTCTGCC TTTGAGACAC CTTCCTTAGC AATCAATTCC 
CGAACGGAAT CTCAACCTCT AGAGGTTAAG ACGGCTCCTA GCACAGAAGA ATCCTCTAAG 
TTACCTCCTG CAGAGACCGA TGATGGCTTC GATGATTTTC AGGAGTTTGC CACTCCTGCG 
AATGGAAACC ACGGTCAAAG CGAAGACGAC GATGATTTTG GCGACTTCGC CGCGCCCGTA 
GTGACTCCTG TTTCTGTACC TCCGCCACCG CCGCCAGCTG CTGTCCATCT GAGCATAGAT 
GAAAGGGTTA AGCCAGTCCT TGATATGATG TTTCCACAAC CAACGGAACT GGACAGCAAA 
AGTATGGACA ATCGCAGGCC TTTGGCTCAG TGCCAGCGAT TCAATTTTGG TGCCATAGAG 
CATGCCCAAG CCCTTGATTA CCAATGGAGC AGCTCGGAAA TGAGACACGC CCTTGTTCGA 
TCGCTGGGCA TCGATTCAAG GAATATACTC TTCGGCGACA AGTGGAACTC ATCGATGCCA 
CGTTTTGCTG CCAACCTGAG CTTTGATCCG CTGAAGCCGC TGAAACCTCA ATCAACCGGC 
GCCGCAAGCA GCCTGGAGCC CATTTCCAAT TCCACCAGCT ACCAGGGATT GGAAAACGCC 
GCCGCAACAA CGGCGAGCCA AACTGAAACG CGGACTGGCC CGTCTCATGG GGAAGGCTCC 
ACCGGCTCCT GTTCCGCCAG CAATTCCGAC GGCGAAGAGC GACCGAGTCA TAGCCACCAG 
CCAACTGCAG TCCCCAGCCC CGAATCGAAC CATATTGAAT CCACAGCAGC GGCATCAGCA 
GCAGCTGTTG CTGCAGCAGC AGCGACGCTA CCGACTACTC TATCAGCAGA CAGCCCTGCC 
CACAAACTCA ACGGCGGTGA GCAGCTGCAT CAGCAGCGGT CGGAGCCGGA GCAGTTTGAT 
TTGAGGACCA CTCCTCCGGC CGCAGCGCCA GCTCAGGAGG AGATCGTGGG CAGCTCGATG 
GCCAGCTATT CGGTGCCGCT GAAGGAGACC CATATCTATA CGCCCTCCAA ATCGGATACG 
GCGGTGGCCA AGACCACCAC ACTAGCGCCC ATTGATTTCG ACTACGAGAT GGCTGCGACG 
GGCATTATCA TCGATGAGAC GGTGGTGAAG AAGGAGTATC GCGACGTGGA GTACAAACCC 
CCATTTGGTC TGGATTCGCC CAGCAAACTG AGTGGCAATG GAGCTGCCAG CAGCTTTCAG 
TTGCCACCAC AGACGACGGA AGATGACGAC TTTAGTGATT TTCAATCTAT GCCGGCGCCA 
AGGTCTCGAA TTGATACACC CACTTTTGGC GAACAGATGA TCCTTAGTCC GGCTATTCTG 
CTGCCCCAAG CCATTCCGCT GGCAAAGAAG CCAGCTGCAA CTATTGAATG GGGCGACAAT 
GCTCTGTCTA GCATAAATGC CGAGGAAATG GCCAGGATAG AGGAGTTGTT CTCCTCGCAG 
GCCAAACCGC TCACCATAAG CGCTTCGGTG GCCAAGAAAC CAGCTCCTTC CACGCCAGCT 
CAGTCTCAGC CTCAAGAAGA CGATGAATGG TCGGACTTTG TCTCTGTTCC AGTCACCCAA 
CAGCAACCGC ATCATCAGCA AAACGCAATT GCTAACAACA ATAATATAGT CAACAGCAAC 
GCTAGGAAAC CAGCTGCTCC CAAAGAAGAT GATTGGTCGG ATTTTGTGAG CAGTACTCCG 
CCAGCACGAC CGGCTCCACA ATTCAATTCC GGTGCGTGGC AGAGCGCTAA TTTCTACAAC 
AATCCCTTGA GTTTGTACCA GGCGCAGCAG CAGCTGCAGC AACAGCCTCA TAGCAATCTA 
GTGCCCAAGG GCAGCACCAG CAGCAGCAAT AACAACAATG TGCCAGCCAT TCCACAGCAA 
ATACACATCA TGCACGACTT CTCGACTGCA CCCGTGTCTG GAGGATTGGG TGTGGGTGCC 
ACCTACCAGC AGCAGCATCA GCGGCAGCAG TTCCAAATCG GCAACGCCAA GGTGGCTCCA 
CGCATCTCCC TGATCCCCGA TCTCAGCTTC GTTGCACCGG CCTTGCCCAC AAATGCTGGT 
GCGTTCATGG GTGCGCTACC GAAACCCACT TTCGGTGGCA AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650355.2
CDS	409..3516
Translation	MVNVPPLLCSTPPPIDFGEEDDDSGLQSTIHLDEGDEYSDFGLVSSSKEPPPPPDPVEEFREKPPDLLEDKQTVEAFNYQVKQKMDYDVFEETAPPTPPPLSLELDDEELEEHVPSLKLDSLSLYSTESVSAASTLSPVAETSAVAPPAMQTTKAHILSHQVTLEDVSDDSDEECSPKKPKELFIREGAVDFFAIEVLATPTLPYGEGFSEENVDTGKGEYVENTSQPEESFRSLEAQEESSNTLEKPMENQSRGLSNPPNQEESPHQWGEPANSDTYSFTNLDATPKDLVHKEIVPEMQEQEDDDDFGDFADFSAAEPQLEITENSTSAFETPSLAINSRTESQPLEVKTAPSTEESSKLPPAETDDGFDDFQEFATPANGNHGQSEDDDDFGDFAAPVVTPVSVPPPPPPAAVHLSIDERVKPVLDMMFPQPTELDSKSMDNRRPLAQCQRFNFGAIEHAQALDYQWSSSEMRHALVRSLGIDSRNILFGDKWNSSMPRFAANLSFDPLKPLKPQSTGAASSLEPISNSTSYQGLENAAATTASQTETRTGPSHGEGSTGSCSASNSDGEERPSHSHQPTAVPSPESNHIESTAAASAAAVAAAAATLPTTLSADSPAHKLNGGEQLHQQRSEPEQFDLRTTPPAAAPAQEEIVGSSMASYSVPLKETHIYTPSKSDTAVAKTTTLAPIDFDYEMAATGIIIDETVVKKEYRDVEYKPPFGLDSPSKLSGNGAASSFQLPPQTTEDDDFSDFQSMPAPRSRIDTPTFGEQMILSPAILLPQAIPLAKKPAATIEWGDNALSSINAEEMARIEELFSSQAKPLTISASVAKKPAPSTPAQSQPQEDDEWSDFVSVPVTQQQPHHQQNAIANNNNIVNSNARKPAAPKEDDWSDFVSSTPPARPAPQFNSGAWQSANFYNNPLSLYQAQQQLQQQPHSNLVPKGSTSSSNNNNVPAIPQQIHIMHDFSTAPVSGGLGVGATYQQQHQRQQFQIGNAKVAPRISLIPDLSFVAPALPTNAGAFMGALPKPTFGGKK

Target ORF information:

RefSeq Version	NM_142098.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster aftiphilin, transcript variant B (Afti), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142098.4

ORF Insert Sequence:

ATGGTGAATG TACCACCGCT GCTCTGCAGC ACCCCCCCGC CCATCGATTT TGGTGAGGAA 
GACGACGATT CCGGTTTACA ATCCACCATC CATTTGGACG AGGGTGACGA ATACTCAGAT 
TTTGGCTTAG TCAGCAGCTC GAAAGAACCC CCTCCACCAC CAGATCCCGT GGAGGAATTT 
CGAGAAAAGC CACCTGATTT GCTGGAAGAC AAACAAACCG TCGAGGCATT TAATTACCAG 
GTGAAACAGA AGATGGATTA CGATGTTTTC GAGGAAACAG CACCGCCGAC GCCGCCTCCG 
TTGAGCTTGG AACTGGATGA TGAGGAACTG GAGGAGCATG TGCCCTCCCT TAAGTTGGAC 
AGTCTAAGTC TCTATTCCAC GGAAAGCGTT TCTGCCGCCT CCACACTCTC ACCGGTTGCG 
GAGACTAGTG CAGTTGCTCC GCCGGCTATG CAAACAACCA AAGCCCATAT CCTCAGCCAC 
CAAGTCACGC TGGAGGATGT GTCCGATGAC AGCGACGAGG AGTGCTCGCC AAAGAAGCCC 
AAAGAACTAT TTATACGCGA GGGAGCTGTG GATTTCTTTG CCATAGAGGT ATTGGCCACT 
CCAACCCTAC CATATGGAGA AGGCTTTTCG GAGGAGAATG TGGATACAGG GAAAGGAGAA 
TACGTAGAGA ATACCTCCCA GCCAGAAGAA AGCTTTAGAA GTTTGGAAGC CCAGGAGGAA 
AGTTCAAATA CCTTAGAAAA GCCTATGGAA AATCAAAGCC GTGGCTTATC AAATCCTCCA 
AATCAGGAGG AATCCCCTCA CCAATGGGGT GAACCAGCAA ATAGTGATAC ATATTCATTT 
ACAAATCTTG ATGCGACGCC AAAAGACTTA GTACACAAAG AAATTGTTCC CGAAATGCAA 
GAGCAAGAAG ATGATGATGA CTTCGGTGAC TTTGCGGATT TCTCAGCAGC AGAACCCCAA 
CTAGAAATCA CGGAGAATTC AACGTCTGCC TTTGAGACAC CTTCCTTAGC AATCAATTCC 
CGAACGGAAT CTCAACCTCT AGAGGTTAAG ACGGCTCCTA GCACAGAAGA ATCCTCTAAG 
TTACCTCCTG CAGAGACCGA TGATGGCTTC GATGATTTTC AGGAGTTTGC CACTCCTGCG 
AATGGAAACC ACGGTCAAAG CGAAGACGAC GATGATTTTG GCGACTTCGC CGCGCCCGTA 
GTGACTCCTG TTTCTGTACC TCCGCCACCG CCGCCAGCTG CTGTCCATCT GAGCATAGAT 
GAAAGGGTTA AGCCAGTCCT TGATATGATG TTTCCACAAC CAACGGAACT GGACAGCAAA 
AGTATGGACA ATCGCAGGCC TTTGGCTCAG TGCCAGCGAT TCAATTTTGG TGCCATAGAG 
CATGCCCAAG CCCTTGATTA CCAATGGAGC AGCTCGGAAA TGAGACACGC CCTTGTTCGA 
TCGCTGGGCA TCGATTCAAG GAATATACTC TTCGGCGACA AGTGGAACTC ATCGATGCCA 
CGTTTTGCTG CCAACCTGAG CTTTGATCCG CTGAAGCCGC TGAAACCTCA ATCAACCGGC 
GCCGCAAGCA GCCTGGAGCC CATTTCCAAT TCCACCAGCT ACCAGGGATT GGAAAACGCC 
GCCGCAACAA CGGCGAGCCA AACTGAAACG CGGACTGGCC CGTCTCATGG GGAAGGCTCC 
ACCGGCTCCT GTTCCGCCAG CAATTCCGAC GGCGAAGAGC GACCGAGTCA TAGCCACCAG 
CCAACTGCAG TCCCCAGCCC CGAATCGAAC CATATTGAAT CCACAGCAGC GGCATCAGCA 
GCAGCTGTTG CTGCAGCAGC AGCGACGCTA CCGACTACTC TATCAGCAGA CAGCCCTGCC 
CACAAACTCA ACGGCGGTGA GCAGCTGCAT CAGCAGCGGT CGGAGCCGGA GCAGTTTGAT 
TTGAGGACCA CTCCTCCGGC CGCAGCGCCA GCTCAGGAGG AGATCGTGGG CAGCTCGATG 
GCCAGCTATT CGGTGCCGCT GAAGGAGACC CATATCTATA CGCCCTCCAA ATCGGATACG 
GCGGTGGCCA AGACCACCAC ACTAGCGCCC ATTGATTTCG ACTACGAGAT GGCTGCGACG 
GGCATTATCA TCGATGAGAC GGTGGTGAAG AAGGAGTATC GCGACGTGGA GTACAAACCC 
CCATTTGGTC TGGATTCGCC CAGCAAACTG AGTGGCAATG GAGCTGCCAG CAGCTTTCAG 
TTGCCACCAC AGACGACGGA AGATGACGAC TTTAGTGATT TTCAATCTAT GCCGGCGCCA 
AGGTCTCGAA TTGATACACC CACTTTTGGC GAACAGATGA TCCTTAGTCC GGCTATTCTG 
CTGCCCCAAG CCATTCCGCT GGCAAAGAAG CCAGCTGCAA CTATTGAATG GGGCGACAAT 
GCTCTGTCTA GCATAAATGC CGAGGAAATG GCCAGGATAG AGGAGTTGTT CTCCTCGCAG 
GCCAAACCGC TCACCATAAG CGCTTCGGTG GCCAAGAAAC CAGCTCCTTC CACGCCAGCT 
CAGTCTCAGC CTCAAGAAGA CGATGAATGG TCGGACTTTG TCTCTGTTCC AGTCACCCAA 
CAGCAACCGC ATCATCAGCA AAACGCAATT GCTAACAACA ATAATATAGT CAACAGCAAC 
GCTAGGAAAC CAGCTGCTCC CAAAGAAGAT GATTGGTCGG ATTTTGTGAG CAGTACTCCG 
CCAGCACGAC CGGCTCCACA ATTCAATTCC GGTGCGTGGC AGAGCGCTAA TTTCTACAAC 
AATCCCTTGA GTTTGTACCA GGCGCAGCAG CAGCTGCAGC AACAGCCTCA TAGCAATCTA 
GTGCCCAAGG GCAGCACCAG CAGCAGCAAT AACAACAATG TGCCAGCCAT TCCACAGCAA 
ATACACATCA TGCACGACTT CTCGACTGCA CCCGTGTCTG GAGGATTGGG TGTGGGTGCC 
ACCTACCAGC AGCAGCATCA GCGGCAGCAG TTCCAAATCG GCAACGCCAA GGTGGCTCCA 
CGCATCTCCC TGATCCCCGA TCTCAGCTTC GTTGCACCGG CCTTGCCCAC AAATGCTGGT 
GCGTTCATGG GTGCGCTACC GAAACCCACT TTCGGTGGCA AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Afti cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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