alpha-Man-IIa cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following alpha-Man-IIa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alpha-Man-IIa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa04327 NM_001275484.1
Latest version!
Drosophila melanogaster alpha-Mannosidase class II a (alpha-Man-IIa), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa08207 NM_079567.3
Latest version!
Drosophila melanogaster alpha-Mannosidase class II a (alpha-Man-IIa), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa04327
    Clone ID Related Accession (Same CDS sequence) NM_001275484.1
    Accession Version NM_001275484.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3330bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product alpha-Mannosidase class II a, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTGCGAA TACGTCGGCG GTTCGCTTTG GTAATTTGCT CCGGCTGCCT GCTGGTTTTC 
    CTCAGCCTGT ACATAATCCT CAATTTTGCG GCGCCGGCAG CCACCCAGAT AAAGCAGCCC
    AACTATGAGA ACATTGAGAA CAAGCTGCAT GAGCTGGAAA ATGGTTTGCA GGAGCACGGG
    GAGGAGATGC GGAATCTCAG GGCGCGTCTT GCCGAAACAT CCAATCGCGA CGATCCAATA
    AGACCTCCAC TTAAAGTGGC TCGTTCCCCG AGGCCAGGGC AATGCCAAGA TGTGGTCCAA
    GACGTGCCCA ATGTGGATGT ACAGATGCTG GAGCTATACG ATCGCATGTC CTTCAAGGAC
    ATAGATGGAG GCGTGTGGAA ACAGGGCTGG AACATTAAGT ACGATCCACT GAAGTACAAC
    GCCCATCACA AACTAAAAGT CTTCGTTGTG CCGCACTCGC ACAACGATCC TGGATGGATT
    CAGACGTTTG AGGAATACTA CCAGCACGAC ACCAAGCACA TCCTGTCCAA TGCACTACGG
    CATCTGCACG ACAATCCCGA GATGAAGTTC ATCTGGGCGG AAATCTCCTA CTTTGCTCGG
    TTCTATCACG ATTTGGGAGA GAACAAAAAG CTGCAGATGA AGTCCATTGT AAAGAATGGA
    CAGTTGGAAT TTGTGACTGG AGGATGGGTA ATGCCGGACG AGGCCAACTC CCACTGGCGA
    AACGTACTGC TGCAGCTGAC CGAAGGGCAA ACATGGTTGA AGCAATTCAT GAATGTCACA
    CCCACTGCTT CCTGGGCCAT CGATCCCTTC GGACACAGTC CCACTATGCC GTACATTTTG
    CAGAAGAGTG GTTTCAAGAA TATGCTTATC CAAAGGACGC ACTATTCGGT TAAGAAGGAA
    CTGGCCCAAC AGCGACAGCT TGAGTTCCTG TGGCGCCAGA TCTGGGACAA CAAAGGGGAC
    ACAGCTCTCT TCACCCACAT GATGCCCTTC TACTCGTACG ACATTCCTCA CACCTGTGGT
    CCAGATCCCA AGGTTTGCTG TCAGTTCGAT TTCAAACGAA TGGGCTCCTT CGGTTTGAGC
    TGTCCATGGA AGGTGCCGCC GCGTACAATC AGTGATCAAA ATGTGGCAGC ACGCTCAGAT
    CTGCTGGTTG ATCAGTGGAA GAAGAAGGCC GAGCTGTATC GCACGAACGT GCTGCTGATT
    CCGTTGGGTG ACGACTTCCG CTTCAAGCAG AACACCGAGT GGGATGTGCA GCGCGTGAAC
    TACGAAAGGC TGTTCGAACA CATCAACAGC CAGGCCCACT TCAATGTCCA GGCGCAGTTC
    GGCACACTGC AGGAATACTT TGATGCAGTG CACCAGGCGG AAAGGGCGGG ACAAGCCGAG
    TTTCCCACGC TAAGCGGTGA CTTTTTCACA TACGCCGATC GATCGGATAA CTATTGGAGT
    GGCTACTACA CATCCCGCCC GTATCATAAG CGCATGGACC GCGTCCTGAT GCACTATGTA
    CGTGCAGCAG AAATGCTTTC CGCCTGGCAC TCCTGGGACG GTATGGCCCG CATCGAGGAA
    CGTCTGGAGC AGGCCCGCAG GGAGCTGTCA TTGTTCCAGC ACCACGACGG TATAACTGGC
    ACAGCAAAAA CGCACGTAGT CGTCGACTAC GAGCAACGCA TGCAGGAAGC TTTAAAAGCC
    TGTCAAATGG TAATGCAACA GTCGGTCTAC CGATTGCTGA CAAAGCCCTC CATCTACAGT
    CCGGACTTCA GTTTCTCGTA CTTTACGCTC GACGACTCCC GCTGGCCAGG ATCTGGTGTG
    GAGGACAGTC GAACCACCAT AATACTGGGC GAGGATATAC TGCCCTCCAA GCATGTGGTG
    ATGCACAACA CCCTGCCCCA CTGGCGGGAG CAGCTGGTGG ACTTTTATGT ATCCAGTCCG
    TTTGTAAGCG TTACCGACTT GGCAAACAAT CCGGTGGAGG CTCAGGTGTC CCCGGTGTGG
    AGCTGGCACC ACGACACACT CACAAAGACT ATCCACCCAC AAGGCTCCAC CACCAAGTAC
    CGCATCATCT TCAAGGCTCG GGTGCCGCCC ATGGGCTTGG CCACCTACGT TTTAACCATC
    TCCGATTCCA AGCCAGAGCA CACCTCGTAT GCATCGAATC TCTTGCTCCG TAAAAACCCG
    ACTTCGTTAC CATTGGGCCA ATATCCGGAG GATGTGAAGT TTGGCGATCC TCGAGAGATC
    TCATTGCGGG TTGGTAACGG ACCCACCTTG GCCTTTTCGG AGCAGGGTCT CCTTAAGTCC
    ATTCAGCTTA CTCAGGATAG CCCACATGTA CCGGTGCACT TCAAGTTCCT CAAGTATGGC
    GTTCGATCGC ATGGCGATAG ATCCGGTGCC TATCTGTTCC TGCCCAATGG ACCAGCTTCG
    CCAGTCGAGC TTGGCCAGCC AGTGGTCCTG GTGACTAAGG GCAAACTGGA GTCGTCCGTG
    AGCGTGGGAC TTCCGAGCGT GGTGCACCAG ACGATAATGC GCGGTGGTGC ACCTGAGATT
    CGCAATCTGG TGGATATAGG CTCACTGGAC AACACGGAGA TCGTGATGCG CTTGGAGACG
    CATATCGACA GCGGCGATAT CTTCTACACG GATCTCAATG GATTGCAATT TATCAAGAGG
    CGGCGTTTGG ACAAATTACC TTTGCAGGCC AACTATTATC CCATACCTTC TGGTATGTTC
    ATTGAGGATG CCAATACGCG ACTCACTCTC CTCACGGGTC AACCGCTGGG TGGATCTTCT
    CTGGCCTCGG GCGAGCTAGA GATTATGCAA GATCGTCGCC TGGCCAGCGA TGATGAACGC
    GGCCTGGGAC AGGGTGTTTT GGACAACAAG CCGGTGCTGC ATATTTATCG GCTGGTGCTG
    GAGAAGGTTA ACAACTGTGT CCGACCGTCA GAGCTTCATC CTGCCGGCTA TTTGACAAGT
    GCCGCTCACA AAGCATCGCA GTCACTGCTG GATCCACTGG ACAAGTTTAT ATTCGCTGAA
    AATGAGTGGA TCGGGGCACA GGGGCAATTT GGTGGCGATC ATCCTTCGGC TCGTGAGGAT
    CTCGATGTGT CGGTGATGAG ACGCTTAACC AAGAGCTCGG CCAAAACCCA GCGAGTAGGC
    TACGTTCTGC ACCGCACCAA TCTGATGCAA TGCGGCACTC CAGAGGAGCA TACACAGAAG
    CTGGATGTGT GCCACCTACT GCCGAATGTG GCGAGATGCG AGCGCACGAC GCTGACTTTC
    CTGCAGAATT TGGAGCACTT GGATGGCATG GTGGCGCCGG AAGTGTGCCC CATGGAAACC
    GCCGCTTATG TGAGCAGTCA CTCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001262413.1
    CDS147..3476
    Translation

    Target ORF information:

    RefSeq Version NM_001275484.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster alpha-Mannosidase class II a (alpha-Man-IIa), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001275484.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTGCGAA TACGTCGGCG GTTCGCTTTG GTAATTTGCT CCGGCTGCCT GCTGGTTTTC 
    CTCAGCCTGT ACATAATCCT CAATTTTGCG GCGCCGGCAG CCACCCAGAT AAAGCAGCCC
    AACTATGAGA ACATTGAGAA CAAGCTGCAT GAGCTGGAAA ATGGTTTGCA GGAGCACGGG
    GAGGAGATGC GGAATCTCAG GGCGCGTCTT GCCGAAACAT CCAATCGCGA CGATCCAATA
    AGACCTCCAC TTAAAGTGGC TCGTTCCCCG AGGCCAGGGC AATGCCAAGA TGTGGTCCAA
    GACGTGCCCA ATGTGGATGT ACAGATGCTG GAGCTATACG ATCGCATGTC CTTCAAGGAC
    ATAGATGGAG GCGTGTGGAA ACAGGGCTGG AACATTAAGT ACGATCCACT GAAGTACAAC
    GCCCATCACA AACTAAAAGT CTTCGTTGTG CCGCACTCGC ACAACGATCC TGGATGGATT
    CAGACGTTTG AGGAATACTA CCAGCACGAC ACCAAGCACA TCCTGTCCAA TGCACTACGG
    CATCTGCACG ACAATCCCGA GATGAAGTTC ATCTGGGCGG AAATCTCCTA CTTTGCTCGG
    TTCTATCACG ATTTGGGAGA GAACAAAAAG CTGCAGATGA AGTCCATTGT AAAGAATGGA
    CAGTTGGAAT TTGTGACTGG AGGATGGGTA ATGCCGGACG AGGCCAACTC CCACTGGCGA
    AACGTACTGC TGCAGCTGAC CGAAGGGCAA ACATGGTTGA AGCAATTCAT GAATGTCACA
    CCCACTGCTT CCTGGGCCAT CGATCCCTTC GGACACAGTC CCACTATGCC GTACATTTTG
    CAGAAGAGTG GTTTCAAGAA TATGCTTATC CAAAGGACGC ACTATTCGGT TAAGAAGGAA
    CTGGCCCAAC AGCGACAGCT TGAGTTCCTG TGGCGCCAGA TCTGGGACAA CAAAGGGGAC
    ACAGCTCTCT TCACCCACAT GATGCCCTTC TACTCGTACG ACATTCCTCA CACCTGTGGT
    CCAGATCCCA AGGTTTGCTG TCAGTTCGAT TTCAAACGAA TGGGCTCCTT CGGTTTGAGC
    TGTCCATGGA AGGTGCCGCC GCGTACAATC AGTGATCAAA ATGTGGCAGC ACGCTCAGAT
    CTGCTGGTTG ATCAGTGGAA GAAGAAGGCC GAGCTGTATC GCACGAACGT GCTGCTGATT
    CCGTTGGGTG ACGACTTCCG CTTCAAGCAG AACACCGAGT GGGATGTGCA GCGCGTGAAC
    TACGAAAGGC TGTTCGAACA CATCAACAGC CAGGCCCACT TCAATGTCCA GGCGCAGTTC
    GGCACACTGC AGGAATACTT TGATGCAGTG CACCAGGCGG AAAGGGCGGG ACAAGCCGAG
    TTTCCCACGC TAAGCGGTGA CTTTTTCACA TACGCCGATC GATCGGATAA CTATTGGAGT
    GGCTACTACA CATCCCGCCC GTATCATAAG CGCATGGACC GCGTCCTGAT GCACTATGTA
    CGTGCAGCAG AAATGCTTTC CGCCTGGCAC TCCTGGGACG GTATGGCCCG CATCGAGGAA
    CGTCTGGAGC AGGCCCGCAG GGAGCTGTCA TTGTTCCAGC ACCACGACGG TATAACTGGC
    ACAGCAAAAA CGCACGTAGT CGTCGACTAC GAGCAACGCA TGCAGGAAGC TTTAAAAGCC
    TGTCAAATGG TAATGCAACA GTCGGTCTAC CGATTGCTGA CAAAGCCCTC CATCTACAGT
    CCGGACTTCA GTTTCTCGTA CTTTACGCTC GACGACTCCC GCTGGCCAGG ATCTGGTGTG
    GAGGACAGTC GAACCACCAT AATACTGGGC GAGGATATAC TGCCCTCCAA GCATGTGGTG
    ATGCACAACA CCCTGCCCCA CTGGCGGGAG CAGCTGGTGG ACTTTTATGT ATCCAGTCCG
    TTTGTAAGCG TTACCGACTT GGCAAACAAT CCGGTGGAGG CTCAGGTGTC CCCGGTGTGG
    AGCTGGCACC ACGACACACT CACAAAGACT ATCCACCCAC AAGGCTCCAC CACCAAGTAC
    CGCATCATCT TCAAGGCTCG GGTGCCGCCC ATGGGCTTGG CCACCTACGT TTTAACCATC
    TCCGATTCCA AGCCAGAGCA CACCTCGTAT GCATCGAATC TCTTGCTCCG TAAAAACCCG
    ACTTCGTTAC CATTGGGCCA ATATCCGGAG GATGTGAAGT TTGGCGATCC TCGAGAGATC
    TCATTGCGGG TTGGTAACGG ACCCACCTTG GCCTTTTCGG AGCAGGGTCT CCTTAAGTCC
    ATTCAGCTTA CTCAGGATAG CCCACATGTA CCGGTGCACT TCAAGTTCCT CAAGTATGGC
    GTTCGATCGC ATGGCGATAG ATCCGGTGCC TATCTGTTCC TGCCCAATGG ACCAGCTTCG
    CCAGTCGAGC TTGGCCAGCC AGTGGTCCTG GTGACTAAGG GCAAACTGGA GTCGTCCGTG
    AGCGTGGGAC TTCCGAGCGT GGTGCACCAG ACGATAATGC GCGGTGGTGC ACCTGAGATT
    CGCAATCTGG TGGATATAGG CTCACTGGAC AACACGGAGA TCGTGATGCG CTTGGAGACG
    CATATCGACA GCGGCGATAT CTTCTACACG GATCTCAATG GATTGCAATT TATCAAGAGG
    CGGCGTTTGG ACAAATTACC TTTGCAGGCC AACTATTATC CCATACCTTC TGGTATGTTC
    ATTGAGGATG CCAATACGCG ACTCACTCTC CTCACGGGTC AACCGCTGGG TGGATCTTCT
    CTGGCCTCGG GCGAGCTAGA GATTATGCAA GATCGTCGCC TGGCCAGCGA TGATGAACGC
    GGCCTGGGAC AGGGTGTTTT GGACAACAAG CCGGTGCTGC ATATTTATCG GCTGGTGCTG
    GAGAAGGTTA ACAACTGTGT CCGACCGTCA GAGCTTCATC CTGCCGGCTA TTTGACAAGT
    GCCGCTCACA AAGCATCGCA GTCACTGCTG GATCCACTGG ACAAGTTTAT ATTCGCTGAA
    AATGAGTGGA TCGGGGCACA GGGGCAATTT GGTGGCGATC ATCCTTCGGC TCGTGAGGAT
    CTCGATGTGT CGGTGATGAG ACGCTTAACC AAGAGCTCGG CCAAAACCCA GCGAGTAGGC
    TACGTTCTGC ACCGCACCAA TCTGATGCAA TGCGGCACTC CAGAGGAGCA TACACAGAAG
    CTGGATGTGT GCCACCTACT GCCGAATGTG GCGAGATGCG AGCGCACGAC GCTGACTTTC
    CTGCAGAATT TGGAGCACTT GGATGGCATG GTGGCGCCGG AAGTGTGCCC CATGGAAACC
    GCCGCTTATG TGAGCAGTCA CTCAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08207
    Clone ID Related Accession (Same CDS sequence) NM_079567.3
    Accession Version NM_079567.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product alpha-Mannosidase class II a, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079567.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTGCGAA TACGTCGGCG GTTCGCTTTG GTAATTTGCT CCGGCTGCCT GCTGGTTTTC 
    CTCAGCCTGT ACATAATCCT CAATTTTGCG GCGCCGGCAG CCACCCAGAT AAAGCCCAAC
    TATGAGAACA TTGAGAACAA GCTGCATGAG CTGGAAAATG GTTTGCAGGA GCACGGGGAG
    GAGATGCGGA ATCTCAGGGC GCGTCTTGCC GAAACATCCA ATCGCGACGA TCCAATAAGA
    CCTCCACTTA AAGTGGCTCG TTCCCCGAGG CCAGGGCAAT GCCAAGATGT GGTCCAAGAC
    GTGCCCAATG TGGATGTACA GATGCTGGAG CTATACGATC GCATGTCCTT CAAGGACATA
    GATGGAGGCG TGTGGAAACA GGGCTGGAAC ATTAAGTACG ATCCACTGAA GTACAACGCC
    CATCACAAAC TAAAAGTCTT CGTTGTGCCG CACTCGCACA ACGATCCTGG ATGGATTCAG
    ACGTTTGAGG AATACTACCA GCACGACACC AAGCACATCC TGTCCAATGC ACTACGGCAT
    CTGCACGACA ATCCCGAGAT GAAGTTCATC TGGGCGGAAA TCTCCTACTT TGCTCGGTTC
    TATCACGATT TGGGAGAGAA CAAAAAGCTG CAGATGAAGT CCATTGTAAA GAATGGACAG
    TTGGAATTTG TGACTGGAGG ATGGGTAATG CCGGACGAGG CCAACTCCCA CTGGCGAAAC
    GTACTGCTGC AGCTGACCGA AGGGCAAACA TGGTTGAAGC AATTCATGAA TGTCACACCC
    ACTGCTTCCT GGGCCATCGA TCCCTTCGGA CACAGTCCCA CTATGCCGTA CATTTTGCAG
    AAGAGTGGTT TCAAGAATAT GCTTATCCAA AGGACGCACT ATTCGGTTAA GAAGGAACTG
    GCCCAACAGC GACAGCTTGA GTTCCTGTGG CGCCAGATCT GGGACAACAA AGGGGACACA
    GCTCTCTTCA CCCACATGAT GCCCTTCTAC TCGTACGACA TTCCTCACAC CTGTGGTCCA
    GATCCCAAGG TTTGCTGTCA GTTCGATTTC AAACGAATGG GCTCCTTCGG TTTGAGCTGT
    CCATGGAAGG TGCCGCCGCG TACAATCAGT GATCAAAATG TGGCAGCACG CTCAGATCTG
    CTGGTTGATC AGTGGAAGAA GAAGGCCGAG CTGTATCGCA CGAACGTGCT GCTGATTCCG
    TTGGGTGACG ACTTCCGCTT CAAGCAGAAC ACCGAGTGGG ATGTGCAGCG CGTGAACTAC
    GAAAGGCTGT TCGAACACAT CAACAGCCAG GCCCACTTCA ATGTCCAGGC GCAGTTCGGC
    ACACTGCAGG AATACTTTGA TGCAGTGCAC CAGGCGGAAA GGGCGGGACA AGCCGAGTTT
    CCCACGCTAA GCGGTGACTT TTTCACATAC GCCGATCGAT CGGATAACTA TTGGAGTGGC
    TACTACACAT CCCGCCCGTA TCATAAGCGC ATGGACCGCG TCCTGATGCA CTATGTACGT
    GCAGCAGAAA TGCTTTCCGC CTGGCACTCC TGGGACGGTA TGGCCCGCAT CGAGGAACGT
    CTGGAGCAGG CCCGCAGGGA GCTGTCATTG TTCCAGCACC ACGACGGTAT AACTGGCACA
    GCAAAAACGC ACGTAGTCGT CGACTACGAG CAACGCATGC AGGAAGCTTT AAAAGCCTGT
    CAAATGGTAA TGCAACAGTC GGTCTACCGA TTGCTGACAA AGCCCTCCAT CTACAGTCCG
    GACTTCAGTT TCTCGTACTT TACGCTCGAC GACTCCCGCT GGCCAGGATC TGGTGTGGAG
    GACAGTCGAA CCACCATAAT ACTGGGCGAG GATATACTGC CCTCCAAGCA TGTGGTGATG
    CACAACACCC TGCCCCACTG GCGGGAGCAG CTGGTGGACT TTTATGTATC CAGTCCGTTT
    GTAAGCGTTA CCGACTTGGC AAACAATCCG GTGGAGGCTC AGGTGTCCCC GGTGTGGAGC
    TGGCACCACG ACACACTCAC AAAGACTATC CACCCACAAG GCTCCACCAC CAAGTACCGC
    ATCATCTTCA AGGCTCGGGT GCCGCCCATG GGCTTGGCCA CCTACGTTTT AACCATCTCC
    GATTCCAAGC CAGAGCACAC CTCGTATGCA TCGAATCTCT TGCTCCGTAA AAACCCGACT
    TCGTTACCAT TGGGCCAATA TCCGGAGGAT GTGAAGTTTG GCGATCCTCG AGAGATCTCA
    TTGCGGGTTG GTAACGGACC CACCTTGGCC TTTTCGGAGC AGGGTCTCCT TAAGTCCATT
    CAGCTTACTC AGGATAGCCC ACATGTACCG GTGCACTTCA AGTTCCTCAA GTATGGCGTT
    CGATCGCATG GCGATAGATC CGGTGCCTAT CTGTTCCTGC CCAATGGACC AGCTTCGCCA
    GTCGAGCTTG GCCAGCCAGT GGTCCTGGTG ACTAAGGGCA AACTGGAGTC GTCCGTGAGC
    GTGGGACTTC CGAGCGTGGT GCACCAGACG ATAATGCGCG GTGGTGCACC TGAGATTCGC
    AATCTGGTGG ATATAGGCTC ACTGGACAAC ACGGAGATCG TGATGCGCTT GGAGACGCAT
    ATCGACAGCG GCGATATCTT CTACACGGAT CTCAATGGAT TGCAATTTAT CAAGAGGCGG
    CGTTTGGACA AATTACCTTT GCAGGCCAAC TATTATCCCA TACCTTCTGG TATGTTCATT
    GAGGATGCCA ATACGCGACT CACTCTCCTC ACGGGTCAAC CGCTGGGTGG ATCTTCTCTG
    GCCTCGGGCG AGCTAGAGAT TATGCAAGAT CGTCGCCTGG CCAGCGATGA TGAACGCGGC
    CTGGGACAGG GTGTTTTGGA CAACAAGCCG GTGCTGCATA TTTATCGGCT GGTGCTGGAG
    AAGGTTAACA ACTGTGTCCG ACCGTCAGAG CTTCATCCTG CCGGCTATTT GACAAGTGCC
    GCTCACAAAG CATCGCAGTC ACTGCTGGAT CCACTGGACA AGTTTATATT CGCTGAAAAT
    GAGTGGATCG GGGCACAGGG GCAATTTGGT GGCGATCATC CTTCGGCTCG TGAGGATCTC
    GATGTGTCGG TGATGAGACG CTTAACCAAG AGCTCGGCCA AAACCCAGCG AGTAGGCTAC
    GTTCTGCACC GCACCAATCT GATGCAATGC GGCACTCCAG AGGAGCATAC ACAGAAGCTG
    GATGTGTGCC ACCTACTGCC GAATGTGGCG AGATGCGAGC GCACGACGCT GACTTTCCTG
    CAGAATTTGG AGCACTTGGA TGGCATGGTG GCGCCGGAAG TGTGCCCCAT GGAAACCGCC
    GCTTATGTGA GCAGTCACTC AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_524291.2
    CDS147..3473
    Translation

    Target ORF information:

    RefSeq Version NM_079567.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster alpha-Mannosidase class II a (alpha-Man-IIa), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_079567.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTGCGAA TACGTCGGCG GTTCGCTTTG GTAATTTGCT CCGGCTGCCT GCTGGTTTTC 
    CTCAGCCTGT ACATAATCCT CAATTTTGCG GCGCCGGCAG CCACCCAGAT AAAGCCCAAC
    TATGAGAACA TTGAGAACAA GCTGCATGAG CTGGAAAATG GTTTGCAGGA GCACGGGGAG
    GAGATGCGGA ATCTCAGGGC GCGTCTTGCC GAAACATCCA ATCGCGACGA TCCAATAAGA
    CCTCCACTTA AAGTGGCTCG TTCCCCGAGG CCAGGGCAAT GCCAAGATGT GGTCCAAGAC
    GTGCCCAATG TGGATGTACA GATGCTGGAG CTATACGATC GCATGTCCTT CAAGGACATA
    GATGGAGGCG TGTGGAAACA GGGCTGGAAC ATTAAGTACG ATCCACTGAA GTACAACGCC
    CATCACAAAC TAAAAGTCTT CGTTGTGCCG CACTCGCACA ACGATCCTGG ATGGATTCAG
    ACGTTTGAGG AATACTACCA GCACGACACC AAGCACATCC TGTCCAATGC ACTACGGCAT
    CTGCACGACA ATCCCGAGAT GAAGTTCATC TGGGCGGAAA TCTCCTACTT TGCTCGGTTC
    TATCACGATT TGGGAGAGAA CAAAAAGCTG CAGATGAAGT CCATTGTAAA GAATGGACAG
    TTGGAATTTG TGACTGGAGG ATGGGTAATG CCGGACGAGG CCAACTCCCA CTGGCGAAAC
    GTACTGCTGC AGCTGACCGA AGGGCAAACA TGGTTGAAGC AATTCATGAA TGTCACACCC
    ACTGCTTCCT GGGCCATCGA TCCCTTCGGA CACAGTCCCA CTATGCCGTA CATTTTGCAG
    AAGAGTGGTT TCAAGAATAT GCTTATCCAA AGGACGCACT ATTCGGTTAA GAAGGAACTG
    GCCCAACAGC GACAGCTTGA GTTCCTGTGG CGCCAGATCT GGGACAACAA AGGGGACACA
    GCTCTCTTCA CCCACATGAT GCCCTTCTAC TCGTACGACA TTCCTCACAC CTGTGGTCCA
    GATCCCAAGG TTTGCTGTCA GTTCGATTTC AAACGAATGG GCTCCTTCGG TTTGAGCTGT
    CCATGGAAGG TGCCGCCGCG TACAATCAGT GATCAAAATG TGGCAGCACG CTCAGATCTG
    CTGGTTGATC AGTGGAAGAA GAAGGCCGAG CTGTATCGCA CGAACGTGCT GCTGATTCCG
    TTGGGTGACG ACTTCCGCTT CAAGCAGAAC ACCGAGTGGG ATGTGCAGCG CGTGAACTAC
    GAAAGGCTGT TCGAACACAT CAACAGCCAG GCCCACTTCA ATGTCCAGGC GCAGTTCGGC
    ACACTGCAGG AATACTTTGA TGCAGTGCAC CAGGCGGAAA GGGCGGGACA AGCCGAGTTT
    CCCACGCTAA GCGGTGACTT TTTCACATAC GCCGATCGAT CGGATAACTA TTGGAGTGGC
    TACTACACAT CCCGCCCGTA TCATAAGCGC ATGGACCGCG TCCTGATGCA CTATGTACGT
    GCAGCAGAAA TGCTTTCCGC CTGGCACTCC TGGGACGGTA TGGCCCGCAT CGAGGAACGT
    CTGGAGCAGG CCCGCAGGGA GCTGTCATTG TTCCAGCACC ACGACGGTAT AACTGGCACA
    GCAAAAACGC ACGTAGTCGT CGACTACGAG CAACGCATGC AGGAAGCTTT AAAAGCCTGT
    CAAATGGTAA TGCAACAGTC GGTCTACCGA TTGCTGACAA AGCCCTCCAT CTACAGTCCG
    GACTTCAGTT TCTCGTACTT TACGCTCGAC GACTCCCGCT GGCCAGGATC TGGTGTGGAG
    GACAGTCGAA CCACCATAAT ACTGGGCGAG GATATACTGC CCTCCAAGCA TGTGGTGATG
    CACAACACCC TGCCCCACTG GCGGGAGCAG CTGGTGGACT TTTATGTATC CAGTCCGTTT
    GTAAGCGTTA CCGACTTGGC AAACAATCCG GTGGAGGCTC AGGTGTCCCC GGTGTGGAGC
    TGGCACCACG ACACACTCAC AAAGACTATC CACCCACAAG GCTCCACCAC CAAGTACCGC
    ATCATCTTCA AGGCTCGGGT GCCGCCCATG GGCTTGGCCA CCTACGTTTT AACCATCTCC
    GATTCCAAGC CAGAGCACAC CTCGTATGCA TCGAATCTCT TGCTCCGTAA AAACCCGACT
    TCGTTACCAT TGGGCCAATA TCCGGAGGAT GTGAAGTTTG GCGATCCTCG AGAGATCTCA
    TTGCGGGTTG GTAACGGACC CACCTTGGCC TTTTCGGAGC AGGGTCTCCT TAAGTCCATT
    CAGCTTACTC AGGATAGCCC ACATGTACCG GTGCACTTCA AGTTCCTCAA GTATGGCGTT
    CGATCGCATG GCGATAGATC CGGTGCCTAT CTGTTCCTGC CCAATGGACC AGCTTCGCCA
    GTCGAGCTTG GCCAGCCAGT GGTCCTGGTG ACTAAGGGCA AACTGGAGTC GTCCGTGAGC
    GTGGGACTTC CGAGCGTGGT GCACCAGACG ATAATGCGCG GTGGTGCACC TGAGATTCGC
    AATCTGGTGG ATATAGGCTC ACTGGACAAC ACGGAGATCG TGATGCGCTT GGAGACGCAT
    ATCGACAGCG GCGATATCTT CTACACGGAT CTCAATGGAT TGCAATTTAT CAAGAGGCGG
    CGTTTGGACA AATTACCTTT GCAGGCCAAC TATTATCCCA TACCTTCTGG TATGTTCATT
    GAGGATGCCA ATACGCGACT CACTCTCCTC ACGGGTCAAC CGCTGGGTGG ATCTTCTCTG
    GCCTCGGGCG AGCTAGAGAT TATGCAAGAT CGTCGCCTGG CCAGCGATGA TGAACGCGGC
    CTGGGACAGG GTGTTTTGGA CAACAAGCCG GTGCTGCATA TTTATCGGCT GGTGCTGGAG
    AAGGTTAACA ACTGTGTCCG ACCGTCAGAG CTTCATCCTG CCGGCTATTT GACAAGTGCC
    GCTCACAAAG CATCGCAGTC ACTGCTGGAT CCACTGGACA AGTTTATATT CGCTGAAAAT
    GAGTGGATCG GGGCACAGGG GCAATTTGGT GGCGATCATC CTTCGGCTCG TGAGGATCTC
    GATGTGTCGG TGATGAGACG CTTAACCAAG AGCTCGGCCA AAACCCAGCG AGTAGGCTAC
    GTTCTGCACC GCACCAATCT GATGCAATGC GGCACTCCAG AGGAGCATAC ACAGAAGCTG
    GATGTGTGCC ACCTACTGCC GAATGTGGCG AGATGCGAGC GCACGACGCT GACTTTCCTG
    CAGAATTTGG AGCACTTGGA TGGCATGGTG GCGCCGGAAG TGTGCCCCAT GGAAACCGCC
    GCTTATGTGA GCAGTCACTC AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.