tou cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following tou gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tou cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa09621 NM_165844.2
Latest version!
Drosophila melanogaster toutatis (tou), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OFa09619 NM_078977.5
Latest version!
Drosophila melanogaster toutatis (tou), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa09620 NM_001103800.3
Latest version!
Drosophila melanogaster toutatis (tou), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa13280 NM_001273970.1
Latest version!
Drosophila melanogaster toutatis (tou), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa13287 NM_001273969.1
Latest version!
Drosophila melanogaster toutatis (tou), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa09621
    Clone ID Related Accession (Same CDS sequence) NM_165844.2
    Accession Version NM_165844.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product toutatis, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_165844.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGCCCGGCG TCTATCCAGT TTATACGCCA GTTGCGGCTG GCAACTTCTC AGCCGGATTG 
    ATTAACCAAG CGCCGGTGCA GCCAGCGATG CCGTTTGCCA ACGTGGTCGC CATGTCGCCA
    CGGGCTGTCA CGCCCACCCG CACCCGAACG CCCACACCGA CGCCAGCACC CACTCCGCCA
    CCACCACCGC CGACACCGCT GCTAATGCAG GCCTCGCCCA CAGCCACCGC CCTCCATGTA
    AACGCCTGCC AGTCACCGCC CCAACAACAG CATGCGCAGC TGATGACCAT GCCCTCGCCA
    CCAGCCATTG GAGTCGGAAC GGCCACTAAC CAAATGTCGC CACCGCCCAT CAACATACAT
    GCCATTCAGG AAGCCAAGGA GAAGCTGAAG CAGGAGAAGA AGGAGAAGCA CGCCACCAAG
    AAGCTCATGA AGGAGCTGGC TGTCTGCAAG ACGCTATTGG GGGAAATGGA GCTTCATGAG
    GACTCGTGGC CATTTCTTTT GCCAGTAAAC ACCAAGCAGT TTCCCACATA TCGAAAAATA
    ATCAAAACTC CCATGGACTT GTCCACAATC AAAAAGAAAT TGCAAGATTT GAGCTACAAA
    ACCCGTGAGG ACTTCTGCGT GGACGTGCGT CAGATCTTCG ATAATTGCGA GATGTTTAAC
    GAGGACGATT CGCCCGTTGG CAAGGCAGGC CACGGCATGC GCAAGTTCTT CGAGTCCCGC
    TGGGGTGAAC TGACTGACAA GCACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_725062.2
    CDS1232..1981
    Translation

    Target ORF information:

    RefSeq Version NM_165844.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster toutatis (tou), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165844.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGCCCGGCG TCTATCCAGT TTATACGCCA GTTGCGGCTG GCAACTTCTC AGCCGGATTG 
    ATTAACCAAG CGCCGGTGCA GCCAGCGATG CCGTTTGCCA ACGTGGTCGC CATGTCGCCA
    CGGGCTGTCA CGCCCACCCG CACCCGAACG CCCACACCGA CGCCAGCACC CACTCCGCCA
    CCACCACCGC CGACACCGCT GCTAATGCAG GCCTCGCCCA CAGCCACCGC CCTCCATGTA
    AACGCCTGCC AGTCACCGCC CCAACAACAG CATGCGCAGC TGATGACCAT GCCCTCGCCA
    CCAGCCATTG GAGTCGGAAC GGCCACTAAC CAAATGTCGC CACCGCCCAT CAACATACAT
    GCCATTCAGG AAGCCAAGGA GAAGCTGAAG CAGGAGAAGA AGGAGAAGCA CGCCACCAAG
    AAGCTCATGA AGGAGCTGGC TGTCTGCAAG ACGCTATTGG GGGAAATGGA GCTTCATGAG
    GACTCGTGGC CATTTCTTTT GCCAGTAAAC ACCAAGCAGT TTCCCACATA TCGAAAAATA
    ATCAAAACTC CCATGGACTT GTCCACAATC AAAAAGAAAT TGCAAGATTT GAGCTACAAA
    ACCCGTGAGG ACTTCTGCGT GGACGTGCGT CAGATCTTCG ATAATTGCGA GATGTTTAAC
    GAGGACGATT CGCCCGTTGG CAAGGCAGGC CACGGCATGC GCAAGTTCTT CGAGTCCCGC
    TGGGGTGAAC TGACTGACAA GCACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa09619
    Clone ID Related Accession (Same CDS sequence) NM_078977.5
    Accession Version NM_078977.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product toutatis, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_078977.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGAACAAAA ATGCCGGCGA TGGCAGCGAT GGCAAAAACT CAAACAAAAA CTCGAATGCG 
    GGAGGGGGTG GTGGAGCCGG GGGGCCACAC GACCCCACCG GCCTTTTAGA TGCAGCTTCC
    CTGTTCGCTT ACTGGGGACG TGATCCAACT GGAGCGGCGG CTGCAGCGGC TTCCAATCCG
    CTCTTCAACT CGCAGTTCAA TGCCGCCGCT GCTGCCGGAT TGGGTTTATT GCCACAAGCT
    GGAGGCGCCT CTGCCAATGA CCGTTATTCC ATGGCAGCAG CAGCGGCGGC TGCGGCGGGA
    GCCCATCACC ACCAGAACAC GATGGCAGTG GCCGCTTCCC AGGCCGCCAG TTTGGCCGGT
    TTGCATCCAG CAAGCTGGTG GTCAATGGCC CAGTTAGCTG CCCAGGATTA CTTCAGTCGC
    CTGCAAGCCT CGGGTCTTTC CCCCTTTCCA CATCCCGATC TGGCGGCTGC CTTTGGACCA
    GCTGGGATGG GAATGGGCGG CGGTGCTGGT GGAGCGGGTG CTGGAGGTGG TGGAGCAGGT
    GTACTCGGCC AGGGCGGCGG TGGAAATGGC GGAAGTGGCA ACGGTGGTGG TGGATCCTCA
    TCGGGTTCTT CCGGCAGCAA GTCAAACAAG TCGCGCAAGG AGAAGCGAGC CGCCCAGCAG
    CAACAACAGC AACAGCAAAA TCTGGCCAAC AGTCTAAATG CGGCAGCTGC GGCGGCAGCA
    GCAGCAGCAG CCAATAATCC AGCAGCCGCG CTGGCCAGCA TGCATGGTTT TGGTGTAGGA
    GTTCCGGGCA CGAGCATTCC GTCGGTGAGC ACAGGTAGCG GATCAATATC CACAAATAGT
    GGAGGTCATG GAAGTTACAA GAGCACGGCC AGCGCTTATG GTAAGCCCTC GACTATGACG
    AGCAGCAGCA TGGCTAATAA TCCGGGCTCT GCCTACGATC CGGTGACCCT GCACAAGGAG
    CTGCTGGCCA TGCAGGCCGT GGCAGCCGCT GCTTCCGGCT CAGGATCAAG CGGTTCCTCA
    GGCAAGAAGT CCGGTGGGGG TGGCTCCTCA TCCTCCTCGA TGCACAGCCT TGGAATGGGA
    ATTGGTGGCA GCGGTGGCAT GATGTCCAGT GGCAAAGCTT CGACCAGTGT GAGCGCAAGC
    AATTCATCAT TGGGAGGAAT GCACCCCAGT TTGACTAGCA GCAATCCGCT GATGTCGCCC
    CATGCGGGCA TGGGCTCCTC GTCGTCATCA TCGAGCAAGG ATCGTGACAA AAACAATCCC
    TCGCTCAACG CTCTTAACTC GCTCTCACAG TTTGGAGCCC TGGGAATGAC GCCACAGCAG
    AGCATGCAGG CGGCAATGAA TGCTTTTGCA GCCAGCACAG GAGTTTCGCC ATCCGCCACG
    GTAACGTCCT CGCCGCACCA CTCTTCCCAG CAGCAACAGC AAATGGGAGG GAACAGTTCC
    ACGTCGGGAT CGGGCAAGTC CAGTTCCAAG GATTACATGA TGGGTACTGG AAGTGAGCAC
    CCATCTCTGC TTGGAGTTCG TTTACCACCG GACACGGAGA TAATCAAGTA CACGTCCTCG
    ATTGTGGGAC CCAAGATTCC TGGTACTACC TCGCGCGGTC GCAAAAAGAC TATTTCCGAA
    ACAGAACAGC AAACAACACA ACAACAACAG AAACAACAAC ACCAAGCAGA ACAACACCTA
    CTCCAACAAC AGCAACAAGC ACAAAAAGAG CTCGACAGCA CCAAAAATGC CATTTCCTCT
    CTGCTTGCAT TTCCAGGTCT CAGTCCCGCG AAGCGGGCTA GACTCGAAAT GGAGTACGCG
    GCGATGGCTG CGGCCGCTCA ACAGCAGCAT CAGCAGGCAC AGCAGCAACA CCAGGCGCAG
    CAGCAACTCC ACGGGATGCT CGGAGCAGCC GGTATCCCGG GAATGGCTGG ACTGGTCGGT
    CTGCCTGGCA TGAGTGGCAA TCCCCTCGAT CAGTTGAGTG TAAGCAAGGC CAGTTCTTCA
    ACGGCGCCCA CAACCAGTAC CAGTTCCTCC TCAGCAGGGA GCAACCTGCT CAACCAGAGC
    AATAGTGATC GCGTGGAGGT AATCAAACTG CCACCAACAA TTACTTCAAA CGGAGCCTAT
    AATCTCTCAA GTAAGGGAAA AGAGGTGCAC GACCTCACCA CCGACATGGC CACCAACTCT
    GGCGGTGTGA ATCTCAGCCT AAAGTCCAAT GCTGGCTCCA GCGCCTTGAC GCCCAGCGGT
    GCCGTCGGAT CGGCCAGCAA TCCCATTACC ATCGATGACT TTGACGCACC ACTTAATCTT
    TCCATGAAGC CCTCGGATAA GAGCAATAGT AGCTCGTCAA ATGCAGCAGC AGGGGGCTCC
    TCCTCTTCCA GCGCAGCGTT GGCTAACTTG GCGAGCGATT ATCAGGCAGC GTCCAGTGGG
    CAAAGCGGTA ATAGTCTGCA GAGTTTGAGC TCTATTACAG CTGCTTTGGG TGGAACAGGA
    GGTATGCCTG GTGGTTCCAT TTCCGGCAGC GGAGGAACCT CTCCAGCTCC AGCTGGTGCC
    GGATCAGGAG CCACGGGTGG TGGTTCGGGA TCTGGTGGAT CCGGCGGTGG ATCGTCCTCT
    TACAAAGAGG GAAGACCTCG TAACCTGGGA CGCGGCGTAT CCAAGCCCAA GAAGAACACG
    GTAGCTTCGC TGTTGGCTCA ATCCCGAGCT GTGGGCCTTA AACCTATGCT GGCCACGCAA
    CAACTTCTTC AACAGGGTGC TGATATTGAG AAAATCCGCC TGGCACTGAG CGAAGCGAAT
    GCCCATATGG AAACTTCTAC AGATTCCGAA AGCGTGGCAG CCGAAAGTGG TCTTTCGGAG
    TCTGAGAGTG AGGATGCCAA CATTCTGAAT GTAGCGGAGC TAAGGGTGCC ACTTGAATTG
    GGCTGGAAAC GGGAGACGGT GATTCGTGGA CTGACTAAGC AAGGGCAGAT CCGTGGAGAG
    GTCACATATT ATGCACCAGG AAGCACGACG CCGCTAAAGA GCAACGGACA GGTTTTTGCT
    ATCCTGGAGC AGCAGCCATC GAATCTAAGT CGTGAAAACT TTAGTTTTTC CGCGCGAGCC
    ATTGTTGGTT CATTCCTGCA GCCTGCACCA CCGCCGTACG CCAATGATGG CGAGTACATA
    CGGATGACGG ACGAGGACGT GGCCAAGCGG TTAGAGGATC TGAAGGTCTT CACCCGCCAG
    ACTCTTAACG TGGAACAGCG AATTGAGATC GCCAAGCAAC AGCAGGCGAT GCGTGATGCT
    AAGAAGCTGC AAAAGGAAGA ATTGGCCAGA AATAAGGAGA AGGCGCGACA GGAGAAGAAC
    TCCAAGTTGG AGCAGCAGCG CAAGGACAAG GAGCTCAAGA ATCAGCAGGC TGTTGAGGAG
    CGCAAAAAGC GTCAGGAGGA GCTAGATCGC CTTAAGCAAG AGGAATTGCT TAAGAAGCAA
    CAGGAGAAAG AAAAGCGTCG TCAGGAGGCC ATTTTAGCTA AGGAACAGGA ACTACAGAAA
    CAAAAAGAGA TGCTGTTGGC TGCCGAAATG GAACGAGAAC GTCGCCGCCA ACACATGAGC
    CTCATTCGAA TGTTGGAGCT TCGTCGGAAA TTCGAGGATC GTGAAAAGAA GAAACACCAG
    CTGGTTCTAG ACCGTTTGCT TCTGCGTGAG CGTCGTATGG CGGAGCGAAA ACGAGATGCG
    GAGATTCTGC AGTTGATAAG GCGTCCCAAT GAGGACTCCG AAATGCCACA AGAATTGGTA
    ATCCCTGAAC TGGATAGGAT TGCCGGCAAC CGACTTCCCG GCCAGGCAAT GGCCGATTTG
    CTCATGGTCT TTGAATTCCT ACATAACTTT GGGGAGACTC TAGGCTTTGA CATGGAATCA
    CTGCCGTCGC TTCAGAACCT GCACGATGCT TTAATGAGCG ATAGCAACGC GGATGCGGAA
    GAGGAGTTGT TGTCGGTGAT GACGCATCTA TTGGTTTGTG CCATTGAGGA TCCAGGTGTT
    CCCAATCCCG GCAGACACAC CACTTTACTG GGACAATCGC TACGCAATGC GGACATCACC
    AACTCGAATG TATCCGAAAT CTTGAGGATT TATCTGTATG CTACGGCCAC AGGTGAAGTG
    CGTCAAATGC ACGGAATCAC TGTGGATCGT GAGCGAGAGA GAAGGGTGCC GGATCATCAT
    CAACTAGATA GCGATACCAC AACCCACTCA CATTCGGTAA AGAACCAGGA GTACTATAAG
    CTTCTCCATG AAAACGACAC CTGGAAGTTG TCGCAATCGC TGAAAGATCG CCCCTTCGTT
    GCGTTAAATC CCACCCGTAA AGCCCAAATG TTGGCCCATC TATGCAACGA CCTGCTCATG
    AACAAGGCCG TGCTCCGCCA GATTGATGGA AGTCTAGAAA CGTGCGCCCA GATGCGAAAG
    GAGAAATACA TGACGGACAT GAAGGTGCGC AAGTACAAGG CTCTCCACAT GCGCAAGGCT
    CGAATTGAGG CCTATGAACG AGCACAGGCG GAGCGTGAGG CGGCTATGCA GGCGTTGATG
    GCACAGCAGA AGCTGGATGC AGAACGTCTT AAGGCGGAGG AGGAAGCCAA GGCCGCGGCG
    GCAGAGGAAG CAGCAGCTGC AGCGGGCACA GATGGAGAGG CAACCAAAGG TGGCTCACCC
    AATGGCGAGA AGCCGGAAGA CGGCGATCAG AATGAAGAGG GAGCCGCCAA GGAACCCCAG
    CAGCAGCAAC AGCAGCCAAT GGAAGTGGAT GGCGTGGTCG ATGAGGCATC TCTTGTAAGT
    CCGGCCAAAA CCATCATTCA AACGGACAAT AGTCTGACGC CCAGTAAACA GGACATGCCC
    ACACCCACCT ACCAAATTAA TGGATCCAGC ACACCTACCA CAAGTGGTGT CACTGGAGGC
    GACATGAATG TTCTGCTGCA GGCCAAGAAA AGCGGGGCCC GAAACTCCAT CAACGATGAA
    CACCACCATG ATGTTAGTAT TATCGACGAT GATCTTTCCG ACTTGGATTC GGAGATCACG
    AATGTCGAGG AGGACGAGGA CAATCGCTTG AGTGCCGATG AGTTGCAAAA GAAGCTCGAC
    AAGATTGTCA GAGCTTCCCT AAACTGCAAG GAGGCACTGG AGAAGAGCAC AAATCAGTTG
    AGAGCTGCGT GCTTTGGGCA GGATCGCTTC TGGCGTCGCT ACTGGAAGTT GCCCAAGGCT
    GGAGGTATTT TCATTGAAGC ACTTGAGTCG GCCCAAAATG ATATTTGCGA TTATCATGAG
    GCATTGGAGG CCATGGATGA CAAAAAGGAT GCAAACGACG AGAAAGAGAA CTCCGAAAAT
    GAGAAGGATG TTGCAGCGGA GTCCAGTGAG CAGCCAATGG AAGTGGATGA GTCCATTACA
    AAACTGGAAG ATGGTGTACC AGCTTCGGAC GTCGGGATGC CTGAAAGTAA TCAACAGAAC
    GCACATCAAG ATGAGGAAGA TGACGATGAT GATGTGACAG AAATCAACAA GGTGGAACCG
    GAAATTGTTG ATTTGGGTGA TGATGACGAT GATGCAGCTC CCCCGCTGCC CAAGATAGAA
    CCTCCCAGGC CAGAGATTAA AGTTAAATCA GAGATGGAGC TGATGGGTCC ACCACCCACG
    ACTATGATAT CCACTAAGAC AGACTTTGAA GCTGAAATTA AAATACCCTC TATGCCTGGC
    ATCTTGATGC CGCCTACCCT CAACAACAAC AACACCAATA ATAACAATAA CAACAATGGA
    AGCGACAACT GTGATAAGTT GGAAACTGGT TTGGGACTAG GACAGCAGCA GCAGAACTTT
    AGCCAGTCTG TGATCAAGAC GGAGGATGTG AAAAAAGAGG ATGATTGCAT AATAGTTTCT
    ACATCCAGTG TTGACGATAC ACCAAAGTGG TTCTCCATTG TCCGGCGGGA GGTTCCATTG
    ATCAGCGAAC TGCCAGCGGA GGAGGGAGGC GTGGTGGGCC AGGAACTTCA GATCAGCTAT
    GCAAATCAGA ACTGCTCCGC TCAGCTGCAG CTGCAAGGTC ATCCGTGGGA TCTGATCAAC
    AACATGCAGT ACTACTCGAT TCCGATGGAC GAATGCAAGG TGGACACCAG CAAGCTGGGC
    AACGAGTGCA TCTTCTCGCT GAGTGGCCTC GATGAAAAGC AAATGCTAGC CAAGGTGGAG
    GAGTACAAGG CCCACAAGGT GGAGTCAAAA AACGGTCTGG GCTCTCCTCA TCGCCACCAC
    GAGACTAAAG ATGATGAGGA GCAGGCCAAG CTGAAGTTGG ACAAGGAGAT TGACACCGAA
    ATGGAAACAG ATGCAGATGA CCTAGCTGGC AAGGAAAAGT TTTTTCGCTT GCGCTCTGAT
    GTACCCCCGG ATACGGGAGG AGGGGTCAGC GAGGGAACCG ATGTAAAGCC CAAGATTGAG
    CTTCGTTTGG ATGAGGCGTT GTCACAGGCA TATTACCACA ACATAGCCAA CATGTCATTG
    AGCAGTGTTC AAACGTATAT ACCCATCGAT ATCCCTCTGC CGCTTTCCAT GACCCCCGAT
    GAGCATCGTC TGCTGGAGCA GGTCAAGCTG GCAGGTTTTC CGGAACGAGT TCACGGAGTT
    TACGTGCCCC GCAGACAGCG CTACGGCTGG TGGCAGTTGG ACGATGAACA GAAGCTGCGC
    CAGCTGCTCA AAACTCTCAA TCCGTCGGGA TTGAGGGAGC GTGAACTGCA GGAGAATCTT
    CAACGATTCC TCGGACTGGA ACAGCCATTG GGTGTGAATT ATAAGCTGAA ATCGGACATC
    GATTTTCCAG AGGAATTCCT AATGCCTGAC AAGAAGGGCG ATTGGAATCC CAAGGTGGCC
    AAACGTGTGG AGCTTGCCCT TATCGAACAA CTAGAATCAC TGGAGGACAA GGTGGCCAGC
    GCTTCTATGC AATTAAAGAA CTGGCAATTG CCCAACCGCG TGGAGAGTGA ACTCACCTTG
    GACTCGCAGG AGGATGTCAC CGAAGAGGAC TTCGTCAGCA TCATACCCAT GATCCGAGAG
    AGAATCATCG ACTTGGAGGC AAATATCGAA AGACGTTACT TGAAGCCGCC TTTGGGCTCG
    CAGACAGGCG ATGCCCATTT GGCGGTTATT GCTCAGAACC AGCATACCAC CACCCAGACG
    CAGAACTCTG CATCGGCAGC AGCATATCTC CTGCAGATGC AACAGCAGCA GCAGCAACAG
    CAGTTGGCCC AGCAGCAGCA GCAACAGCAA CAGGGTTCGG GTGCGGGAAA TAGCCTGAAT
    CCCTCATCCT TCAACGAGCG TACTATGGCA TTGGCGGCGG CAGCAGCTGC TTCGGGGCCA
    GGAAACGCAA CCGGAGTAGC CAACTCGGCA GTCGTGGCAG GAGCCACGCC CTGCGAATCG
    GGCAGCGGAG AACCGAATTC CGGCAACGCG TCACCGGCCA GCAACTGTGA CAGCGATCGG
    GACGAGAAGG TGGAGCAGAT ACCCAAGGGA TTGGTGCAGT GGCGGGACGC AGTATCCCGA
    TCGCACACCA CCGCCCAGCT GGCGATGGCC CTGTACGTTC TGGAATCCTG CGTGGCTTGG
    GACAAGAGCA TTATGAAAGC GAATTGCCAG TTCTGCACGT CCGGCGAAAA CGAGGACAAG
    TTGCTACTGT GCGACGGCTG TGACAAGGGC TATCACACCT ATTGCTTCAA GCCCAAGATG
    GACAACATTC CCGATGGCGA TTGGTACTGC TACGAGTGCG TGAACAAGGC CACCAATGAG
    CGCAAGTGCA TCGTTTGCGG CGGTCATCGT CCGTCGCCCG TGGGGAAGAT GATCTACTGT
    GACTTGTGTC CTCGTGCCTA CCACGCCGAT TGCTATATAC CGCCGCTGCT AAAGGTACCG
    CGTGGCAAGT GGTACTGCCA CGGTTGCATC TCCCGGGCGC CTCCGCCGAA GAAAAGAAGT
    GCAGGTGGAA CATCCGGCAG CAGCAGCAAA TCTAGGAGGG ACAGGGATAG GGAGTCGGGC
    GGATCGGCCA AGCGGCGTAG CGACAACAGC AAGACACCAG CCATGGAGCA CATGCAGCAG
    CAGCAGATGC CACTGGCAGG CGGGGATTCC CACCATCATC ATCATCAGCA GCCGCCGTCG
    CTGAACTCCT CGCACGATGA GTCGATGAAT TCTCTGCCGG CGGCTCCTCT GAGTCCGGCG
    CACTCGGTGG TCTCGGCCAC GAACTACGAT GACCAGCACC ATGCCAACAA CTCGGTCGAT
    GGCAGCAGTC GCTTCCACGC GCATCTCATT CCCCCGTCAA ATAATGGCAC TGCGGCTCTG
    CTGGAAGATG TGCCGGGTGG CGCCAATGTG ATGCCCGGCG TCTATCCAGT TTATACGCCA
    GTTGCGGCTG GCAACTTCTC AGCCGGATTG ATTAACCAAG CGCCGGTGCA GCCAGCGATG
    CCGTTTGCCA ACGTGGTCGC CATGTCGCCA CGGGCTGTCA CGCCCACCCG CACCCGAACG
    CCCACACCGA CGCCAGCACC CACTCCGCCA CCACCACCGC CGACACCGCT GCTAATGCAG
    GCCTCGCCCA CAGCCACCGC CCTCCATGTA AACGCCTGCC AGTCACCGCC CCAACAACAG
    CATGCGCAGC TGATGACCAT GCCCTCGCCA CCAGCCATTG GAGTCGGAAC GGCCACTAAC
    CAAATGTCGC CACCGCCCAT CAACATACAT GCCATTCAGG AAGCCAAGGA GAAGCTGAAG
    CAGGAGAAGA AGGAGAAGCA CGCCACCAAG AAGCTCATGA AGGAGCTGGC TGTCTGCAAG
    ACGCTATTGG GGGAAATGGA GCTTCATGAG GACTCGTGGC CATTTCTTTT GCCAGTAAAC
    ACCAAGCAGT TTCCCACATA TCGAAAAATA ATCAAAACTC CCATGGACTT GTCCACAATC
    AAAAAGAAAT TGCAAGATTT GAGCTACAAA ACCCGTGAGG ACTTCTGCGT GGACGTGCGT
    CAGATCTTCG ATAATTGCGA GATGTTTAAC GAGGACGATT CGCCCGTTGG CAAGGCAGGC
    CACGGCATGC GCAAGTTCTT CGAGTCCCGC TGGGGTGAAC TGACTGACAA GCACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_523701.3
    CDS524..9523
    Translation

    Target ORF information:

    RefSeq Version NM_078977.5
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster toutatis (tou), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_078977.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGAACAAAA ATGCCGGCGA TGGCAGCGAT GGCAAAAACT CAAACAAAAA CTCGAATGCG 
    GGAGGGGGTG GTGGAGCCGG GGGGCCACAC GACCCCACCG GCCTTTTAGA TGCAGCTTCC
    CTGTTCGCTT ACTGGGGACG TGATCCAACT GGAGCGGCGG CTGCAGCGGC TTCCAATCCG
    CTCTTCAACT CGCAGTTCAA TGCCGCCGCT GCTGCCGGAT TGGGTTTATT GCCACAAGCT
    GGAGGCGCCT CTGCCAATGA CCGTTATTCC ATGGCAGCAG CAGCGGCGGC TGCGGCGGGA
    GCCCATCACC ACCAGAACAC GATGGCAGTG GCCGCTTCCC AGGCCGCCAG TTTGGCCGGT
    TTGCATCCAG CAAGCTGGTG GTCAATGGCC CAGTTAGCTG CCCAGGATTA CTTCAGTCGC
    CTGCAAGCCT CGGGTCTTTC CCCCTTTCCA CATCCCGATC TGGCGGCTGC CTTTGGACCA
    GCTGGGATGG GAATGGGCGG CGGTGCTGGT GGAGCGGGTG CTGGAGGTGG TGGAGCAGGT
    GTACTCGGCC AGGGCGGCGG TGGAAATGGC GGAAGTGGCA ACGGTGGTGG TGGATCCTCA
    TCGGGTTCTT CCGGCAGCAA GTCAAACAAG TCGCGCAAGG AGAAGCGAGC CGCCCAGCAG
    CAACAACAGC AACAGCAAAA TCTGGCCAAC AGTCTAAATG CGGCAGCTGC GGCGGCAGCA
    GCAGCAGCAG CCAATAATCC AGCAGCCGCG CTGGCCAGCA TGCATGGTTT TGGTGTAGGA
    GTTCCGGGCA CGAGCATTCC GTCGGTGAGC ACAGGTAGCG GATCAATATC CACAAATAGT
    GGAGGTCATG GAAGTTACAA GAGCACGGCC AGCGCTTATG GTAAGCCCTC GACTATGACG
    AGCAGCAGCA TGGCTAATAA TCCGGGCTCT GCCTACGATC CGGTGACCCT GCACAAGGAG
    CTGCTGGCCA TGCAGGCCGT GGCAGCCGCT GCTTCCGGCT CAGGATCAAG CGGTTCCTCA
    GGCAAGAAGT CCGGTGGGGG TGGCTCCTCA TCCTCCTCGA TGCACAGCCT TGGAATGGGA
    ATTGGTGGCA GCGGTGGCAT GATGTCCAGT GGCAAAGCTT CGACCAGTGT GAGCGCAAGC
    AATTCATCAT TGGGAGGAAT GCACCCCAGT TTGACTAGCA GCAATCCGCT GATGTCGCCC
    CATGCGGGCA TGGGCTCCTC GTCGTCATCA TCGAGCAAGG ATCGTGACAA AAACAATCCC
    TCGCTCAACG CTCTTAACTC GCTCTCACAG TTTGGAGCCC TGGGAATGAC GCCACAGCAG
    AGCATGCAGG CGGCAATGAA TGCTTTTGCA GCCAGCACAG GAGTTTCGCC ATCCGCCACG
    GTAACGTCCT CGCCGCACCA CTCTTCCCAG CAGCAACAGC AAATGGGAGG GAACAGTTCC
    ACGTCGGGAT CGGGCAAGTC CAGTTCCAAG GATTACATGA TGGGTACTGG AAGTGAGCAC
    CCATCTCTGC TTGGAGTTCG TTTACCACCG GACACGGAGA TAATCAAGTA CACGTCCTCG
    ATTGTGGGAC CCAAGATTCC TGGTACTACC TCGCGCGGTC GCAAAAAGAC TATTTCCGAA
    ACAGAACAGC AAACAACACA ACAACAACAG AAACAACAAC ACCAAGCAGA ACAACACCTA
    CTCCAACAAC AGCAACAAGC ACAAAAAGAG CTCGACAGCA CCAAAAATGC CATTTCCTCT
    CTGCTTGCAT TTCCAGGTCT CAGTCCCGCG AAGCGGGCTA GACTCGAAAT GGAGTACGCG
    GCGATGGCTG CGGCCGCTCA ACAGCAGCAT CAGCAGGCAC AGCAGCAACA CCAGGCGCAG
    CAGCAACTCC ACGGGATGCT CGGAGCAGCC GGTATCCCGG GAATGGCTGG ACTGGTCGGT
    CTGCCTGGCA TGAGTGGCAA TCCCCTCGAT CAGTTGAGTG TAAGCAAGGC CAGTTCTTCA
    ACGGCGCCCA CAACCAGTAC CAGTTCCTCC TCAGCAGGGA GCAACCTGCT CAACCAGAGC
    AATAGTGATC GCGTGGAGGT AATCAAACTG CCACCAACAA TTACTTCAAA CGGAGCCTAT
    AATCTCTCAA GTAAGGGAAA AGAGGTGCAC GACCTCACCA CCGACATGGC CACCAACTCT
    GGCGGTGTGA ATCTCAGCCT AAAGTCCAAT GCTGGCTCCA GCGCCTTGAC GCCCAGCGGT
    GCCGTCGGAT CGGCCAGCAA TCCCATTACC ATCGATGACT TTGACGCACC ACTTAATCTT
    TCCATGAAGC CCTCGGATAA GAGCAATAGT AGCTCGTCAA ATGCAGCAGC AGGGGGCTCC
    TCCTCTTCCA GCGCAGCGTT GGCTAACTTG GCGAGCGATT ATCAGGCAGC GTCCAGTGGG
    CAAAGCGGTA ATAGTCTGCA GAGTTTGAGC TCTATTACAG CTGCTTTGGG TGGAACAGGA
    GGTATGCCTG GTGGTTCCAT TTCCGGCAGC GGAGGAACCT CTCCAGCTCC AGCTGGTGCC
    GGATCAGGAG CCACGGGTGG TGGTTCGGGA TCTGGTGGAT CCGGCGGTGG ATCGTCCTCT
    TACAAAGAGG GAAGACCTCG TAACCTGGGA CGCGGCGTAT CCAAGCCCAA GAAGAACACG
    GTAGCTTCGC TGTTGGCTCA ATCCCGAGCT GTGGGCCTTA AACCTATGCT GGCCACGCAA
    CAACTTCTTC AACAGGGTGC TGATATTGAG AAAATCCGCC TGGCACTGAG CGAAGCGAAT
    GCCCATATGG AAACTTCTAC AGATTCCGAA AGCGTGGCAG CCGAAAGTGG TCTTTCGGAG
    TCTGAGAGTG AGGATGCCAA CATTCTGAAT GTAGCGGAGC TAAGGGTGCC ACTTGAATTG
    GGCTGGAAAC GGGAGACGGT GATTCGTGGA CTGACTAAGC AAGGGCAGAT CCGTGGAGAG
    GTCACATATT ATGCACCAGG AAGCACGACG CCGCTAAAGA GCAACGGACA GGTTTTTGCT
    ATCCTGGAGC AGCAGCCATC GAATCTAAGT CGTGAAAACT TTAGTTTTTC CGCGCGAGCC
    ATTGTTGGTT CATTCCTGCA GCCTGCACCA CCGCCGTACG CCAATGATGG CGAGTACATA
    CGGATGACGG ACGAGGACGT GGCCAAGCGG TTAGAGGATC TGAAGGTCTT CACCCGCCAG
    ACTCTTAACG TGGAACAGCG AATTGAGATC GCCAAGCAAC AGCAGGCGAT GCGTGATGCT
    AAGAAGCTGC AAAAGGAAGA ATTGGCCAGA AATAAGGAGA AGGCGCGACA GGAGAAGAAC
    TCCAAGTTGG AGCAGCAGCG CAAGGACAAG GAGCTCAAGA ATCAGCAGGC TGTTGAGGAG
    CGCAAAAAGC GTCAGGAGGA GCTAGATCGC CTTAAGCAAG AGGAATTGCT TAAGAAGCAA
    CAGGAGAAAG AAAAGCGTCG TCAGGAGGCC ATTTTAGCTA AGGAACAGGA ACTACAGAAA
    CAAAAAGAGA TGCTGTTGGC TGCCGAAATG GAACGAGAAC GTCGCCGCCA ACACATGAGC
    CTCATTCGAA TGTTGGAGCT TCGTCGGAAA TTCGAGGATC GTGAAAAGAA GAAACACCAG
    CTGGTTCTAG ACCGTTTGCT TCTGCGTGAG CGTCGTATGG CGGAGCGAAA ACGAGATGCG
    GAGATTCTGC AGTTGATAAG GCGTCCCAAT GAGGACTCCG AAATGCCACA AGAATTGGTA
    ATCCCTGAAC TGGATAGGAT TGCCGGCAAC CGACTTCCCG GCCAGGCAAT GGCCGATTTG
    CTCATGGTCT TTGAATTCCT ACATAACTTT GGGGAGACTC TAGGCTTTGA CATGGAATCA
    CTGCCGTCGC TTCAGAACCT GCACGATGCT TTAATGAGCG ATAGCAACGC GGATGCGGAA
    GAGGAGTTGT TGTCGGTGAT GACGCATCTA TTGGTTTGTG CCATTGAGGA TCCAGGTGTT
    CCCAATCCCG GCAGACACAC CACTTTACTG GGACAATCGC TACGCAATGC GGACATCACC
    AACTCGAATG TATCCGAAAT CTTGAGGATT TATCTGTATG CTACGGCCAC AGGTGAAGTG
    CGTCAAATGC ACGGAATCAC TGTGGATCGT GAGCGAGAGA GAAGGGTGCC GGATCATCAT
    CAACTAGATA GCGATACCAC AACCCACTCA CATTCGGTAA AGAACCAGGA GTACTATAAG
    CTTCTCCATG AAAACGACAC CTGGAAGTTG TCGCAATCGC TGAAAGATCG CCCCTTCGTT
    GCGTTAAATC CCACCCGTAA AGCCCAAATG TTGGCCCATC TATGCAACGA CCTGCTCATG
    AACAAGGCCG TGCTCCGCCA GATTGATGGA AGTCTAGAAA CGTGCGCCCA GATGCGAAAG
    GAGAAATACA TGACGGACAT GAAGGTGCGC AAGTACAAGG CTCTCCACAT GCGCAAGGCT
    CGAATTGAGG CCTATGAACG AGCACAGGCG GAGCGTGAGG CGGCTATGCA GGCGTTGATG
    GCACAGCAGA AGCTGGATGC AGAACGTCTT AAGGCGGAGG AGGAAGCCAA GGCCGCGGCG
    GCAGAGGAAG CAGCAGCTGC AGCGGGCACA GATGGAGAGG CAACCAAAGG TGGCTCACCC
    AATGGCGAGA AGCCGGAAGA CGGCGATCAG AATGAAGAGG GAGCCGCCAA GGAACCCCAG
    CAGCAGCAAC AGCAGCCAAT GGAAGTGGAT GGCGTGGTCG ATGAGGCATC TCTTGTAAGT
    CCGGCCAAAA CCATCATTCA AACGGACAAT AGTCTGACGC CCAGTAAACA GGACATGCCC
    ACACCCACCT ACCAAATTAA TGGATCCAGC ACACCTACCA CAAGTGGTGT CACTGGAGGC
    GACATGAATG TTCTGCTGCA GGCCAAGAAA AGCGGGGCCC GAAACTCCAT CAACGATGAA
    CACCACCATG ATGTTAGTAT TATCGACGAT GATCTTTCCG ACTTGGATTC GGAGATCACG
    AATGTCGAGG AGGACGAGGA CAATCGCTTG AGTGCCGATG AGTTGCAAAA GAAGCTCGAC
    AAGATTGTCA GAGCTTCCCT AAACTGCAAG GAGGCACTGG AGAAGAGCAC AAATCAGTTG
    AGAGCTGCGT GCTTTGGGCA GGATCGCTTC TGGCGTCGCT ACTGGAAGTT GCCCAAGGCT
    GGAGGTATTT TCATTGAAGC ACTTGAGTCG GCCCAAAATG ATATTTGCGA TTATCATGAG
    GCATTGGAGG CCATGGATGA CAAAAAGGAT GCAAACGACG AGAAAGAGAA CTCCGAAAAT
    GAGAAGGATG TTGCAGCGGA GTCCAGTGAG CAGCCAATGG AAGTGGATGA GTCCATTACA
    AAACTGGAAG ATGGTGTACC AGCTTCGGAC GTCGGGATGC CTGAAAGTAA TCAACAGAAC
    GCACATCAAG ATGAGGAAGA TGACGATGAT GATGTGACAG AAATCAACAA GGTGGAACCG
    GAAATTGTTG ATTTGGGTGA TGATGACGAT GATGCAGCTC CCCCGCTGCC CAAGATAGAA
    CCTCCCAGGC CAGAGATTAA AGTTAAATCA GAGATGGAGC TGATGGGTCC ACCACCCACG
    ACTATGATAT CCACTAAGAC AGACTTTGAA GCTGAAATTA AAATACCCTC TATGCCTGGC
    ATCTTGATGC CGCCTACCCT CAACAACAAC AACACCAATA ATAACAATAA CAACAATGGA
    AGCGACAACT GTGATAAGTT GGAAACTGGT TTGGGACTAG GACAGCAGCA GCAGAACTTT
    AGCCAGTCTG TGATCAAGAC GGAGGATGTG AAAAAAGAGG ATGATTGCAT AATAGTTTCT
    ACATCCAGTG TTGACGATAC ACCAAAGTGG TTCTCCATTG TCCGGCGGGA GGTTCCATTG
    ATCAGCGAAC TGCCAGCGGA GGAGGGAGGC GTGGTGGGCC AGGAACTTCA GATCAGCTAT
    GCAAATCAGA ACTGCTCCGC TCAGCTGCAG CTGCAAGGTC ATCCGTGGGA TCTGATCAAC
    AACATGCAGT ACTACTCGAT TCCGATGGAC GAATGCAAGG TGGACACCAG CAAGCTGGGC
    AACGAGTGCA TCTTCTCGCT GAGTGGCCTC GATGAAAAGC AAATGCTAGC CAAGGTGGAG
    GAGTACAAGG CCCACAAGGT GGAGTCAAAA AACGGTCTGG GCTCTCCTCA TCGCCACCAC
    GAGACTAAAG ATGATGAGGA GCAGGCCAAG CTGAAGTTGG ACAAGGAGAT TGACACCGAA
    ATGGAAACAG ATGCAGATGA CCTAGCTGGC AAGGAAAAGT TTTTTCGCTT GCGCTCTGAT
    GTACCCCCGG ATACGGGAGG AGGGGTCAGC GAGGGAACCG ATGTAAAGCC CAAGATTGAG
    CTTCGTTTGG ATGAGGCGTT GTCACAGGCA TATTACCACA ACATAGCCAA CATGTCATTG
    AGCAGTGTTC AAACGTATAT ACCCATCGAT ATCCCTCTGC CGCTTTCCAT GACCCCCGAT
    GAGCATCGTC TGCTGGAGCA GGTCAAGCTG GCAGGTTTTC CGGAACGAGT TCACGGAGTT
    TACGTGCCCC GCAGACAGCG CTACGGCTGG TGGCAGTTGG ACGATGAACA GAAGCTGCGC
    CAGCTGCTCA AAACTCTCAA TCCGTCGGGA TTGAGGGAGC GTGAACTGCA GGAGAATCTT
    CAACGATTCC TCGGACTGGA ACAGCCATTG GGTGTGAATT ATAAGCTGAA ATCGGACATC
    GATTTTCCAG AGGAATTCCT AATGCCTGAC AAGAAGGGCG ATTGGAATCC CAAGGTGGCC
    AAACGTGTGG AGCTTGCCCT TATCGAACAA CTAGAATCAC TGGAGGACAA GGTGGCCAGC
    GCTTCTATGC AATTAAAGAA CTGGCAATTG CCCAACCGCG TGGAGAGTGA ACTCACCTTG
    GACTCGCAGG AGGATGTCAC CGAAGAGGAC TTCGTCAGCA TCATACCCAT GATCCGAGAG
    AGAATCATCG ACTTGGAGGC AAATATCGAA AGACGTTACT TGAAGCCGCC TTTGGGCTCG
    CAGACAGGCG ATGCCCATTT GGCGGTTATT GCTCAGAACC AGCATACCAC CACCCAGACG
    CAGAACTCTG CATCGGCAGC AGCATATCTC CTGCAGATGC AACAGCAGCA GCAGCAACAG
    CAGTTGGCCC AGCAGCAGCA GCAACAGCAA CAGGGTTCGG GTGCGGGAAA TAGCCTGAAT
    CCCTCATCCT TCAACGAGCG TACTATGGCA TTGGCGGCGG CAGCAGCTGC TTCGGGGCCA
    GGAAACGCAA CCGGAGTAGC CAACTCGGCA GTCGTGGCAG GAGCCACGCC CTGCGAATCG
    GGCAGCGGAG AACCGAATTC CGGCAACGCG TCACCGGCCA GCAACTGTGA CAGCGATCGG
    GACGAGAAGG TGGAGCAGAT ACCCAAGGGA TTGGTGCAGT GGCGGGACGC AGTATCCCGA
    TCGCACACCA CCGCCCAGCT GGCGATGGCC CTGTACGTTC TGGAATCCTG CGTGGCTTGG
    GACAAGAGCA TTATGAAAGC GAATTGCCAG TTCTGCACGT CCGGCGAAAA CGAGGACAAG
    TTGCTACTGT GCGACGGCTG TGACAAGGGC TATCACACCT ATTGCTTCAA GCCCAAGATG
    GACAACATTC CCGATGGCGA TTGGTACTGC TACGAGTGCG TGAACAAGGC CACCAATGAG
    CGCAAGTGCA TCGTTTGCGG CGGTCATCGT CCGTCGCCCG TGGGGAAGAT GATCTACTGT
    GACTTGTGTC CTCGTGCCTA CCACGCCGAT TGCTATATAC CGCCGCTGCT AAAGGTACCG
    CGTGGCAAGT GGTACTGCCA CGGTTGCATC TCCCGGGCGC CTCCGCCGAA GAAAAGAAGT
    GCAGGTGGAA CATCCGGCAG CAGCAGCAAA TCTAGGAGGG ACAGGGATAG GGAGTCGGGC
    GGATCGGCCA AGCGGCGTAG CGACAACAGC AAGACACCAG CCATGGAGCA CATGCAGCAG
    CAGCAGATGC CACTGGCAGG CGGGGATTCC CACCATCATC ATCATCAGCA GCCGCCGTCG
    CTGAACTCCT CGCACGATGA GTCGATGAAT TCTCTGCCGG CGGCTCCTCT GAGTCCGGCG
    CACTCGGTGG TCTCGGCCAC GAACTACGAT GACCAGCACC ATGCCAACAA CTCGGTCGAT
    GGCAGCAGTC GCTTCCACGC GCATCTCATT CCCCCGTCAA ATAATGGCAC TGCGGCTCTG
    CTGGAAGATG TGCCGGGTGG CGCCAATGTG ATGCCCGGCG TCTATCCAGT TTATACGCCA
    GTTGCGGCTG GCAACTTCTC AGCCGGATTG ATTAACCAAG CGCCGGTGCA GCCAGCGATG
    CCGTTTGCCA ACGTGGTCGC CATGTCGCCA CGGGCTGTCA CGCCCACCCG CACCCGAACG
    CCCACACCGA CGCCAGCACC CACTCCGCCA CCACCACCGC CGACACCGCT GCTAATGCAG
    GCCTCGCCCA CAGCCACCGC CCTCCATGTA AACGCCTGCC AGTCACCGCC CCAACAACAG
    CATGCGCAGC TGATGACCAT GCCCTCGCCA CCAGCCATTG GAGTCGGAAC GGCCACTAAC
    CAAATGTCGC CACCGCCCAT CAACATACAT GCCATTCAGG AAGCCAAGGA GAAGCTGAAG
    CAGGAGAAGA AGGAGAAGCA CGCCACCAAG AAGCTCATGA AGGAGCTGGC TGTCTGCAAG
    ACGCTATTGG GGGAAATGGA GCTTCATGAG GACTCGTGGC CATTTCTTTT GCCAGTAAAC
    ACCAAGCAGT TTCCCACATA TCGAAAAATA ATCAAAACTC CCATGGACTT GTCCACAATC
    AAAAAGAAAT TGCAAGATTT GAGCTACAAA ACCCGTGAGG ACTTCTGCGT GGACGTGCGT
    CAGATCTTCG ATAATTGCGA GATGTTTAAC GAGGACGATT CGCCCGTTGG CAAGGCAGGC
    CACGGCATGC GCAAGTTCTT CGAGTCCCGC TGGGGTGAAC TGACTGACAA GCACTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa09620
    Clone ID Related Accession (Same CDS sequence) NM_001103800.3
    Accession Version NM_001103800.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product toutatis, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001103800.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGAACAAAA ATGCCGGCGA TGGCAGCGAT GGCAAAAACT CAAACAAAAA CTCGAATGCG 
    GGAGGGGGTG GTGGAGCCGG GGGGCCACAC GACCCCACCG GCCTTTTAGA TGCAGCTTCC
    CTGTTCGCTT ACTGGGGACG TGATCCAACT GGAGCGGCGG CTGCAGCGGC TTCCAATCCG
    CTCTTCAACT CGCAGTTCAA TGCCGCCGCT GCTGCCGGAT TGGGTTTATT GCCACAAGCT
    GGAGGCGCCT CTGCCAATGA CCGTTATTCC ATGGCAGCAG CAGCGGCGGC TGCGGCGGGA
    GCCCATCACC ACCAGAACAC GATGGCAGTG GCCGCTTCCC AGGCCGCCAG TTTGGCCGGT
    TTGCATCCAG CAAGCTGGTG GTCAATGGCC CAGTTAGCTG CCCAGGATTA CTTCAGTCGC
    CTGCAAGCCT CGGGTCTTTC CCCCTTTCCA CATCCCGATC TGGCGGCTGC CTTTGGACCA
    GCTGGGATGG GAATGGGCGG CGGTGCTGGT GGAGCGGGTG CTGGAGGTGG TGGAGCAGGT
    GTACTCGGCC AGGGCGGCGG TGGAAATGGC GGAAGTGGCA ACGGTGGTGG TGGATCCTCA
    TCGGGTTCTT CCGGCAGCAA GTCAAACAAG TCGCGCAAGG AGAAGCGAGC CGCCCAGCAG
    CAACAACAGC AACAGCAAAA TCTGGCCAAC AGTCTAAATG CGGCAGCTGC GGCGGCAGCA
    GCAGCAGCAG CCAATAATCC AGCAGCCGCG CTGGCCAGCA TGCATGGTTT TGGTGTAGGA
    GTTCCGGGCA CGAGCATTCC GTCGGTGAGC ACAGGTAGCG GATCAATATC CACAAATAGT
    GGAGGTCATG GAAGTTACAA GAGCACGGCC AGCGCTTATG GTAAGCCCTC GACTATGACG
    AGCAGCAGCA TGGCTAATAA TCCGGGCTCT GCCTACGATC CGGTGACCCT GCACAAGGAG
    CTGCTGGCCA TGCAGGCCGT GGCAGCCGCT GCTTCCGGCT CAGGATCAAG CGGTTCCTCA
    GGCAAGAAGT CCGGTGGGGG TGGCTCCTCA TCCTCCTCGA TGCACAGCCT TGGAATGGGA
    ATTGGTGGCA GCGGTGGCAT GATGTCCAGT GGCAAAGCTT CGACCAGTGT GAGCGCAAGC
    AATTCATCAT TGGGAGGAAT GCACCCCAGT TTGACTAGCA GCAATCCGCT GATGTCGCCC
    CATGCGGGCA TGGGCTCCTC GTCGTCATCA TCGAGCAAGG ATCGTGACAA AAACAATCCC
    TCGCTCAACG CTCTTAACTC GCTCTCACAG TTTGGAGCCC TGGGAATGAC GCCACAGCAG
    AGCATGCAGG CGGCAATGAA TGCTTTTGCA GCCAGCACAG GAGTTTCGCC ATCCGCCACG
    GTAACGTCCT CGCCGCACCA CTCTTCCCAG CAGCAACAGC AAATGGGAGG GAACAGTTCC
    ACGTCGGGAT CGGGCAAGTC CAGTTCCAAG GATTACATGA TGGGTACTGG AAGTGAGCAC
    CCATCTCTGC TTGGAGTTCG TTTACCACCG GACACGGAGA TAATCAAGTA CACGTCCTCG
    ATTGTGGGAC CCAAGATTCC TGGTACTACC TCGCGCGGTC GCAAAAAGAC TATTTCCGAA
    ACAGAACAGC AAACAACACA ACAACAACAG AAACAACAAC ACCAAGCAGA ACAACACCTA
    CTCCAACAAC AGCAACAAGC ACAAAAAGAG CTCGACAGCA CCAAAAATGC CATTTCCTCT
    CTGCTTGCAT TTCCAGGTCT CAGTCCCGCG AAGCGGGCTA GACTCGAAAT GGAGTACGCG
    GCGATGGCTG CGGCCGCTCA ACAGCAGCAT CAGCAGGCAC AGCAGCAACA CCAGGCGCAG
    CAGCAACTCC ACGGGATGCT CGGAGCAGCC GGTATCCCGG GAATGGCTGG ACTGGTCGGT
    CTGCCTGGCA TGAGTGGCAA TCCCCTCGAT CAGTTGAGTG TAAGCAAGGC CAGTTCTTCA
    ACGGCGCCCA CAACCAGTAC CAGTTCCTCC TCAGCAGGGA GCAACCTGCT CAACCAGAGC
    AATAGTGATC GCGTGGAGGT AATCAAACTG CCACCAACAA TTACTTCAAA CGGAGCCTAT
    AATCTCTCAA GTAAGGGAAA AGAGGTGCAC GACCTCACCA CCGACATGGC CACCAACTCT
    GGCGGTGTGA ATCTCAGCCT AAAGTCCAAT GCTGGCTCCA GCGCCTTGAC GCCCAGCGGT
    GCCGTCGGAT CGGCCAGCAA TCCCATTACC ATCGATGACT TTGACGCACC ACTTAATCTT
    TCCATGAAGC CCTCGGATAA GAGCAATAGT AGCTCGTCAA ATGCAGCAGC AGGGGGCTCC
    TCCTCTTCCA GCGCAGCGTT GGCTAACTTG GCGAGCGATT ATCAGGCAGC GTCCAGTGGG
    CAAAGCGGTA ATAGTCTGCA GAGTTTGAGC TCTATTACAG CTGCTTTGGG TGGAACAGGA
    GGTATGCCTG GTGGTTCCAT TTCCGGCAGC GGAGGAACCT CTCCAGCTCC AGCTGGTGCC
    GGATCAGGAG CCACGGGTGG TGGTTCGGGA TCTGGTGGAT CCGGCGGTGG ATCGTCCTCT
    TACAAAGAGG GAAGACCTCG TAACCTGGGA CGCGGCGTAT CCAAGCCCAA GAAGAACACG
    GTAGCTTCGC TGTTGGCTCA ATCCCGAGCT GTGGGCCTTA AACCTATGCT GGCCACGCAA
    CAACTTCTTC AACAGGGTGC TGATATTGAG AAAATCCGCC TGGCACTGAG CGAAGCGAAT
    GCCCATATGG AAACTTCTAC AGATTCCGAA AGCGTGGCAG CCGAAAGTGG TCTTTCGGAG
    TCTGAGAGTG AGGATGCCAA CATTCTGAAT GTAGCGGAGC TAAGGGTGCC ACTTGAATTG
    GGCTGGAAAC GGGAGACGGT GATTCGTGGA CTGACTAAGC AAGGGCAGAT CCGTGGAGAG
    GTCACATATT ATGCACCAGG AAGCACGACG CCGCTAAAGA GCAACGGACA GGTTTTTGCT
    ATCCTGGAGC AGCAGCCATC GAATCTAAGT CGTGAAAACT TTAGTTTTTC CGCGCGAGCC
    ATTGTTGGTT CATTCCTGCA GCCTGCACCA CCGCCGTACG CCAATGATGG CGAGTACATA
    CGGATGACGG ACGAGGACGT GGCCAAGCGG TTAGAGGATC TGAAGGTCTT CACCCGCCAG
    ACTCTTAACG TGGAACAGCG AATTGAGATC GCCAAGCAAC AGCAGGCGAT GCGTGATGCT
    AAGAAGCTGC AAAAGGAAGA ATTGGCCAGA AATAAGGAGA AGGCGCGACA GGAGAAGAAC
    TCCAAGTTGG AGCAGCAGCG CAAGGACAAG GAGCTCAAGA ATCAGCAGGC TGTTGAGGAG
    CGCAAAAAGC GTCAGGAGGA GCTAGATCGC CTTAAGCAAG AGGAATTGCT TAAGAAGCAA
    CAGGAGAAAG AAAAGCGTCG TCAGGAGGCC ATTTTAGCTA AGGAACAGGA ACTACAGAAA
    CAAAAAGAGA TGCTGTTGGC TGCCGAAATG GAACGAGAAC GTCGCCGCCA ACACATGAGC
    CTCATTCGAA TGTTGGAGCT TCGTCGGAAA TTCGAGGATC GTGAAAAGAA GAAACACCAG
    CTGGTTCTAG ACCGTTTGCT TCTGCGTGAG CGTCGTATGG CGGAGCGAAA ACGAGATGCG
    GAGATTCTGC AGTTGATAAG GCGTCCCAAT GAGGACTCCG AAATGCCACA AGAATTGGTA
    ATCCCTGAAC TGGATAGGAT TGCCGGCAAC CGACTTCCCG GCCAGGCAAT GGCCGATTTG
    CTCATGGTCT TTGAATTCCT ACATAACTTT GGGGAGACTC TAGGCTTTGA CATGGAATCA
    CTGCCGTCGC TTCAGAACCT GCACGATGCT TTAATGAGCG ATAGCAACGC GGATGCGGAA
    GAGGAGTTGT TGTCGGTGAT GACGCATCTA TTGGTTTGTG CCATTGAGGA TCCAGGTGTT
    CCCAATCCCG GCAGACACAC CACTTTACTG GGACAATCGC TACGCAATGC GGACATCACC
    AACTCGAATG TATCCGAAAT CTTGAGGATT TATCTGTATG CTACGGCCAC AGGTGAAGTG
    CGTCAAATGC ACGGAATCAC TGTGGATCGT GAGCGAGAGA GAAGGGTGCC GGATCATCAT
    CAACTAGATA GCGATACCAC AACCCACTCA CATTCGGTAA AGAACCAGGA GTACTATAAG
    CTTCTCCATG AAAACGACAC CTGGAAGTTG TCGCAATCGC TGAAAGATCG CCCCTTCGTT
    GCGTTAAATC CCACCCGTAA AGCCCAAATG TTGGCCCATC TATGCAACGA CCTGCTCATG
    AACAAGGCCG TGCTCCGCCA GATTGATGGA AGTCTAGAAA CGTGCGCCCA GATGCGAAAG
    GAGAAATACA TGACGGACAT GAAGGTGCGC AAGTACAAGG CTCTCCACAT GCGCAAGGCT
    CGAATTGAGG CCTATGAACG AGCACAGGCG GAGCGTGAGG CGGCTATGCA GGCGTTGATG
    GCACAGCAGA AGCTGGATGC AGAACGTCTT AAGGCGGAGG AGGAAGCCAA GGCCGCGGCG
    GCAGAGGAAG CAGCAGCTGC AGCGGGCACA GATGGAGAGG CAACCAAAGG TGGCTCACCC
    AATGGCGAGA AGCCGGAAGA CGGCGATCAG AATGAAGAGG GAGCCGCCAA GGAACCCCAG
    CAGCAGCAAC AGCAGCCAAT GGAAGTGGAT GGCGTGGTCG ATGAGGCATC TCTTGTAAGT
    CCGGCCAAAA CCATCATTCA AACGGACAAT AGTCTGACGC CCAGTAAACA GGACATGCCC
    ACACCCACCT ACCAAATTAA TGGATCCAGC ACACCTACCA CAAGTGGTGT CACTGGAGGC
    GACATGAATG TTCTGCTGCA GGCCAAGAAA AGCGGGGCCC GAAACTCCAT CAACGATGAA
    CACCACCATG ATGTTAGTAT TATCGACGAT GATCTTTCCG ACTTGGATTC GGAGATCACG
    AATGTCGAGG AGGACGAGGA CAATCGCTTG AGTGCCGATG AGTTGCAAAA GAAGCTCGAC
    AAGATTGTCA GAGCTTCCCT AAACTGCAAG GAGGCACTGG AGAAGAGCAC AAATCAGTTG
    AGAGCTGCGT GCTTTGGGCA GGATCGCTTC TGGCGTCGCT ACTGGAAGTT GCCCAAGGCT
    GGAGGTATTT TCATTGAAGC ACTTGAGTCG GCCCAAAATG ATATTTGCGA TTATCATGAG
    GCATTGGAGG CCATGGATGA CAAAAAGGAT GCAAACGACG AGAAAGAGAA CTCCGAAAAT
    GAGAAGGATG TTGCAGCGGA GTCCAGTGAG CAGCCAATGG AAGTGGATGA GTCCATTACA
    AAACTGGAAG ATGGTGTACC AGCTTCGGAC GTCGGGATGC CTGAAAGTAA TCAACAGAAC
    GCACATCAAG ATGAGGAAGA TGACGATGAT GATGTGACAG AAATCAACAA GGTGGAACCG
    GAAATTGTTG ATTTGGGTGA TGATGACGAT GATGCAGCTC CCCCGCTGCC CAAGATAGAA
    CCTCCCAGGC CAGAGATTAA AGTTAAATCA GAGATGGAGC TGATGGGTCC ACCACCCACG
    ACTATGATAT CCACTAAGAC AGACTTTGAA GCTGAAATTA AAATACCCTC TATGCCTGGC
    ATCTTGATGC CGCCTACCCT CAACAACAAC AACACCAATA ATAACAATAA CAACAATGGA
    AGCGACAACT GTGATAAGTT GGAAACTGGT TTGGGACTAG GACAGCAGCA GCAGAACTTT
    AGCCAGTCTG TGATCAAGAC GGAGGATGTG AAAAAAGAGG ATGATTGCAT AATAGTTTCT
    ACATCCAGTG TTGACGATAC ACCAAAGTGG TTCTCCATTG TCCGGCGGGA GGTTCCATTG
    ATCAGCGAAC TGCCAGCGGA GGAGGGAGGC GTGGTGGGCC AGGAACTTCA GATCAGCTAT
    GCAAATCAGA ACTGCTCCGC TCAGCTGCAG CTGCAAGGTC ATCCGTGGGA TCTGATCAAC
    AACATGCAGT ACTACTCGAT TCCGATGGAC GAATGCAAGG TGGACACCAG CAAGCTGGGC
    AACGAGTGCA TCTTCTCGCT GAGTGGCCTC GATGAAAAGC AAATGCTAGC CAAGGTGGAG
    GAGTACAAGG CCCACAAGGT GGAGTCAAAA AACGGTCTGG GCTCTCCTCA TCGCCACCAC
    GAGACTAAAG ATGATGAGGA GCAGGCCAAG CTGAAGTTGG ACAAGGAGAT TGACACCGAA
    ATGGAAACAG ATGCAGATGA CCTAGCTGGC AAGGAAAAGT TTTTTCGCTT GCGCTCTGAT
    GTACCCCCGG ATACGGGAGG AGGGGTCAGC GAGGGAACCG ATGTAAAGCC CAAGATTGAG
    CTTCGTTTGG ATGAGGCGTT GTCACAGGCA TATTACCACA ACATAGCCAA CATGTCATTG
    AGCAGTGTTC AAACGTATAT ACCCATCGAT ATCCCTCTGC CGCTTTCCAT GACCCCCGAT
    GAGCATCGTC TGCTGGAGCA GGTCAAGCTG GCAGGTTTTC CGGAACGAGT TCACGGAGTT
    TACGTGCCCC GCAGACAGCG CTACGGCTGG TGGCAGTTGG ACGATGAACA GAAGCTGCGC
    CAGCTGCTCA AAACTCTCAA TCCGTCGGGA TTGAGGGAGC GTGAACTGCA GGAGAATCTT
    CAACGATTCC TCGGACTGGA ACAGCCATTG GGTGTGAATT ATAAGCTGAA ATCGGACATC
    GATTTTCCAG AGGAATTCCT AATGCCTGAC AAGAAGGGCG ATTGGAATCC CAAGGTGGCC
    AAACGTGTGG AGCTTGCCCT TATCGAACAA CTAGAATCAC TGGAGGACAA GGTGGCCAGC
    GCTTCTATGC AATTAAAGAA CTGGCAATTG CCCAACCGCG TGGAGAGTGA ACTCACCTTG
    GACTCGCAGG AGGATGTCAC CGAAGAGGAC TTCGTCAGCA TCATACCCAT GATCCGAGAG
    AGAATCATCG ACTTGGAGGC AAATATCGAA AGACGTTACT TGAAGCCGCC TTTGGGCTCG
    CAGACAGGCG ATGCCCATTT GGCGGTTATT GCTCAGAACC AGCATACCAC CACCCAGACG
    CAGAACTCTG CATCGGCAGC AGCATATCTC CTGCAGATGC AACAGCAGCA GCAGCAACAG
    CAGTTGGCCC AGCAGCAGCA GCAACAGCAA CAGGGTTCGG GTGCGGGAAA TAGCCTGAAT
    CCCTCATCCT TCAACGAGCG TACTATGGCA TTGGCGGCGG CAGCAGCTGC TTCGGGGCCA
    GGAAACGCAA CCGGAGTAGC CAACTCGGCA GTCGTGGCAG GAGCCACGCC CTGCGAATCG
    GGCAGCGGAG AACCGAATTC CGGCAACGCG TCACCGGCCA GCAACTGTGA CAGCGATCGG
    GACGAGAAGG TGGAGCAGAT ACCCAAGGGA TTGGTGCAGT GGCGGGACGC AGTATCCCGA
    TCGCACACCA CCGCCCAGCT GGCGATGGCC CTGTACGTTC TGGAATCCTG CGTGGCTTGG
    GACAAGAGCA TTATGAAAGC GTATGCAACA AAAAACAAGT CCAGCAAAAA GAAGAGCAGC
    GCCAAAAAGC AGGCAACTCC CTCGAAGAAA CAGCAGCAGA AGAAAAACAA AAAGGAGCAG
    CAGTTGACCC CCAATGGAAA AGAGAGCAAG AGCAAGAGCG CAATCAATAA GCCAGAAAAC
    CAGGCTCCGC TGAGCATCAA AATCAACCTT AAGGCTCTGG CCCAAAACGG GCAGTGTTTG
    CTGAAAACTA AAACACCACC AATCCTAACC AAATCCCCAA CCGCATCCCC ATCCTCCCAT
    CCAAACACTA GCGACTCCGA CTCGGACTTT GGCAAACGGA CGAAGAAGAA GAGCGGCGGC
    AAACGCAGAC GCTCGGCCAA CAATACAAAC TCTAGCAAAT ATTCCAATTC CTTACAGAAT
    TGCCAGTTCT GCACGTCCGG CGAAAACGAG GACAAGTTGC TACTGTGCGA CGGCTGTGAC
    AAGGGCTATC ACACCTATTG CTTCAAGCCC AAGATGGACA ACATTCCCGA TGGCGATTGG
    TACTGCTACG AGTGCGTGAA CAAGGCCACC AATGAGCGCA AGTGCATCGT TTGCGGCGGT
    CATCGTCCGT CGCCCGTGGG GAAGATGATC TACTGTGACT TGTGTCCTCG TGCCTACCAC
    GCCGATTGCT ATATACCGCC GCTGCTAAAG GTACCGCGTG GCAAGTGGTA CTGCCACGGT
    TGCATCTCCC GGGCGCCTCC GCCGAAGAAA AGAAGTGCAG GTGGAACATC CGGCAGCAGC
    AGCAAATCTA GGAGGGACAG GGATAGGGAG TCGGGCGGAT CGGCCAAGCG GCGTAGCGAC
    AACAGCAAGA CACCAGCCAT GGAGCACATG CAGCAGCAGC AGATGCCACT GGCAGGCGGG
    GATTCCCACC ATCATCATCA TCAGCAGCCG CCGTCGCTGA ACTCCTCGCA CGATGAGTCG
    ATGAATTCTC TGCCGGCGGC TCCTCTGAGT CCGGCGCACT CGGTGGTCTC GGCCACGAAC
    TACGATGACC AGCACCATGC CAACAACTCG GTCGATGGCA GCAGTCGCTT CCACGCGCAT
    CTCATTCCCC CGTCAAATAA TGGCACTGCG GCTCTGCTGG AAGATGTGCC GGGTGGCGCC
    AATGTGATGC CCGGCGTCTA TCCAGTTTAT ACGCCAGTTG CGGCTGGCAA CTTCTCAGCC
    GGATTGATTA ACCAAGCGCC GGTGCAGCCA GCGATGCCGT TTGCCAACGT GGTCGCCATG
    TCGCCACGGG CTGTCACGCC CACCCGCACC CGAACGCCCA CACCGACGCC AGCACCCACT
    CCGCCACCAC CACCGCCGAC ACCGCTGCTA ATGCAGGCCT CGCCCACAGC CACCGCCCTC
    CATGTAAACG CCTGCCAGTC ACCGCCCCAA CAACAGCATG CGCAGCTGAT GACCATGCCC
    TCGCCACCAG CCATTGGAGT CGGAACGGCC ACTAACCAAA TGTCGCCACC GCCCATCAAC
    ATACATGCCA TTCAGGAAGC CAAGGAGAAG CTGAAGCAGG AGAAGAAGGA GAAGCACGCC
    ACCAAGAAGC TCATGAAGGA GCTGGCTGTC TGCAAGACGC TATTGGGGGA AATGGAGCTT
    CATGAGGACT CGTGGCCATT TCTTTTGCCA GTAAACACCA AGCAGTTTCC CACATATCGA
    AAAATAATCA AAACTCCCAT GGACTTGTCC ACAATCAAAA AGAAATTGCA AGATTTGAGC
    TACAAAACCC GTGAGGACTT CTGCGTGGAC GTGCGTCAGA TCTTCGATAA TTGCGAGATG
    TTTAACGAGG ACGATTCGCC CGTTGGCAAG GCAGGCCACG GCATGCGCAA GTTCTTCGAG
    TCCCGCTGGG GTGAACTGAC TGACAAGCAC TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001097270.1
    CDS524..9919
    Translation

    Target ORF information:

    RefSeq Version NM_001103800.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster toutatis (tou), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103800.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGAACAAAA ATGCCGGCGA TGGCAGCGAT GGCAAAAACT CAAACAAAAA CTCGAATGCG 
    GGAGGGGGTG GTGGAGCCGG GGGGCCACAC GACCCCACCG GCCTTTTAGA TGCAGCTTCC
    CTGTTCGCTT ACTGGGGACG TGATCCAACT GGAGCGGCGG CTGCAGCGGC TTCCAATCCG
    CTCTTCAACT CGCAGTTCAA TGCCGCCGCT GCTGCCGGAT TGGGTTTATT GCCACAAGCT
    GGAGGCGCCT CTGCCAATGA CCGTTATTCC ATGGCAGCAG CAGCGGCGGC TGCGGCGGGA
    GCCCATCACC ACCAGAACAC GATGGCAGTG GCCGCTTCCC AGGCCGCCAG TTTGGCCGGT
    TTGCATCCAG CAAGCTGGTG GTCAATGGCC CAGTTAGCTG CCCAGGATTA CTTCAGTCGC
    CTGCAAGCCT CGGGTCTTTC CCCCTTTCCA CATCCCGATC TGGCGGCTGC CTTTGGACCA
    GCTGGGATGG GAATGGGCGG CGGTGCTGGT GGAGCGGGTG CTGGAGGTGG TGGAGCAGGT
    GTACTCGGCC AGGGCGGCGG TGGAAATGGC GGAAGTGGCA ACGGTGGTGG TGGATCCTCA
    TCGGGTTCTT CCGGCAGCAA GTCAAACAAG TCGCGCAAGG AGAAGCGAGC CGCCCAGCAG
    CAACAACAGC AACAGCAAAA TCTGGCCAAC AGTCTAAATG CGGCAGCTGC GGCGGCAGCA
    GCAGCAGCAG CCAATAATCC AGCAGCCGCG CTGGCCAGCA TGCATGGTTT TGGTGTAGGA
    GTTCCGGGCA CGAGCATTCC GTCGGTGAGC ACAGGTAGCG GATCAATATC CACAAATAGT
    GGAGGTCATG GAAGTTACAA GAGCACGGCC AGCGCTTATG GTAAGCCCTC GACTATGACG
    AGCAGCAGCA TGGCTAATAA TCCGGGCTCT GCCTACGATC CGGTGACCCT GCACAAGGAG
    CTGCTGGCCA TGCAGGCCGT GGCAGCCGCT GCTTCCGGCT CAGGATCAAG CGGTTCCTCA
    GGCAAGAAGT CCGGTGGGGG TGGCTCCTCA TCCTCCTCGA TGCACAGCCT TGGAATGGGA
    ATTGGTGGCA GCGGTGGCAT GATGTCCAGT GGCAAAGCTT CGACCAGTGT GAGCGCAAGC
    AATTCATCAT TGGGAGGAAT GCACCCCAGT TTGACTAGCA GCAATCCGCT GATGTCGCCC
    CATGCGGGCA TGGGCTCCTC GTCGTCATCA TCGAGCAAGG ATCGTGACAA AAACAATCCC
    TCGCTCAACG CTCTTAACTC GCTCTCACAG TTTGGAGCCC TGGGAATGAC GCCACAGCAG
    AGCATGCAGG CGGCAATGAA TGCTTTTGCA GCCAGCACAG GAGTTTCGCC ATCCGCCACG
    GTAACGTCCT CGCCGCACCA CTCTTCCCAG CAGCAACAGC AAATGGGAGG GAACAGTTCC
    ACGTCGGGAT CGGGCAAGTC CAGTTCCAAG GATTACATGA TGGGTACTGG AAGTGAGCAC
    CCATCTCTGC TTGGAGTTCG TTTACCACCG GACACGGAGA TAATCAAGTA CACGTCCTCG
    ATTGTGGGAC CCAAGATTCC TGGTACTACC TCGCGCGGTC GCAAAAAGAC TATTTCCGAA
    ACAGAACAGC AAACAACACA ACAACAACAG AAACAACAAC ACCAAGCAGA ACAACACCTA
    CTCCAACAAC AGCAACAAGC ACAAAAAGAG CTCGACAGCA CCAAAAATGC CATTTCCTCT
    CTGCTTGCAT TTCCAGGTCT CAGTCCCGCG AAGCGGGCTA GACTCGAAAT GGAGTACGCG
    GCGATGGCTG CGGCCGCTCA ACAGCAGCAT CAGCAGGCAC AGCAGCAACA CCAGGCGCAG
    CAGCAACTCC ACGGGATGCT CGGAGCAGCC GGTATCCCGG GAATGGCTGG ACTGGTCGGT
    CTGCCTGGCA TGAGTGGCAA TCCCCTCGAT CAGTTGAGTG TAAGCAAGGC CAGTTCTTCA
    ACGGCGCCCA CAACCAGTAC CAGTTCCTCC TCAGCAGGGA GCAACCTGCT CAACCAGAGC
    AATAGTGATC GCGTGGAGGT AATCAAACTG CCACCAACAA TTACTTCAAA CGGAGCCTAT
    AATCTCTCAA GTAAGGGAAA AGAGGTGCAC GACCTCACCA CCGACATGGC CACCAACTCT
    GGCGGTGTGA ATCTCAGCCT AAAGTCCAAT GCTGGCTCCA GCGCCTTGAC GCCCAGCGGT
    GCCGTCGGAT CGGCCAGCAA TCCCATTACC ATCGATGACT TTGACGCACC ACTTAATCTT
    TCCATGAAGC CCTCGGATAA GAGCAATAGT AGCTCGTCAA ATGCAGCAGC AGGGGGCTCC
    TCCTCTTCCA GCGCAGCGTT GGCTAACTTG GCGAGCGATT ATCAGGCAGC GTCCAGTGGG
    CAAAGCGGTA ATAGTCTGCA GAGTTTGAGC TCTATTACAG CTGCTTTGGG TGGAACAGGA
    GGTATGCCTG GTGGTTCCAT TTCCGGCAGC GGAGGAACCT CTCCAGCTCC AGCTGGTGCC
    GGATCAGGAG CCACGGGTGG TGGTTCGGGA TCTGGTGGAT CCGGCGGTGG ATCGTCCTCT
    TACAAAGAGG GAAGACCTCG TAACCTGGGA CGCGGCGTAT CCAAGCCCAA GAAGAACACG
    GTAGCTTCGC TGTTGGCTCA ATCCCGAGCT GTGGGCCTTA AACCTATGCT GGCCACGCAA
    CAACTTCTTC AACAGGGTGC TGATATTGAG AAAATCCGCC TGGCACTGAG CGAAGCGAAT
    GCCCATATGG AAACTTCTAC AGATTCCGAA AGCGTGGCAG CCGAAAGTGG TCTTTCGGAG
    TCTGAGAGTG AGGATGCCAA CATTCTGAAT GTAGCGGAGC TAAGGGTGCC ACTTGAATTG
    GGCTGGAAAC GGGAGACGGT GATTCGTGGA CTGACTAAGC AAGGGCAGAT CCGTGGAGAG
    GTCACATATT ATGCACCAGG AAGCACGACG CCGCTAAAGA GCAACGGACA GGTTTTTGCT
    ATCCTGGAGC AGCAGCCATC GAATCTAAGT CGTGAAAACT TTAGTTTTTC CGCGCGAGCC
    ATTGTTGGTT CATTCCTGCA GCCTGCACCA CCGCCGTACG CCAATGATGG CGAGTACATA
    CGGATGACGG ACGAGGACGT GGCCAAGCGG TTAGAGGATC TGAAGGTCTT CACCCGCCAG
    ACTCTTAACG TGGAACAGCG AATTGAGATC GCCAAGCAAC AGCAGGCGAT GCGTGATGCT
    AAGAAGCTGC AAAAGGAAGA ATTGGCCAGA AATAAGGAGA AGGCGCGACA GGAGAAGAAC
    TCCAAGTTGG AGCAGCAGCG CAAGGACAAG GAGCTCAAGA ATCAGCAGGC TGTTGAGGAG
    CGCAAAAAGC GTCAGGAGGA GCTAGATCGC CTTAAGCAAG AGGAATTGCT TAAGAAGCAA
    CAGGAGAAAG AAAAGCGTCG TCAGGAGGCC ATTTTAGCTA AGGAACAGGA ACTACAGAAA
    CAAAAAGAGA TGCTGTTGGC TGCCGAAATG GAACGAGAAC GTCGCCGCCA ACACATGAGC
    CTCATTCGAA TGTTGGAGCT TCGTCGGAAA TTCGAGGATC GTGAAAAGAA GAAACACCAG
    CTGGTTCTAG ACCGTTTGCT TCTGCGTGAG CGTCGTATGG CGGAGCGAAA ACGAGATGCG
    GAGATTCTGC AGTTGATAAG GCGTCCCAAT GAGGACTCCG AAATGCCACA AGAATTGGTA
    ATCCCTGAAC TGGATAGGAT TGCCGGCAAC CGACTTCCCG GCCAGGCAAT GGCCGATTTG
    CTCATGGTCT TTGAATTCCT ACATAACTTT GGGGAGACTC TAGGCTTTGA CATGGAATCA
    CTGCCGTCGC TTCAGAACCT GCACGATGCT TTAATGAGCG ATAGCAACGC GGATGCGGAA
    GAGGAGTTGT TGTCGGTGAT GACGCATCTA TTGGTTTGTG CCATTGAGGA TCCAGGTGTT
    CCCAATCCCG GCAGACACAC CACTTTACTG GGACAATCGC TACGCAATGC GGACATCACC
    AACTCGAATG TATCCGAAAT CTTGAGGATT TATCTGTATG CTACGGCCAC AGGTGAAGTG
    CGTCAAATGC ACGGAATCAC TGTGGATCGT GAGCGAGAGA GAAGGGTGCC GGATCATCAT
    CAACTAGATA GCGATACCAC AACCCACTCA CATTCGGTAA AGAACCAGGA GTACTATAAG
    CTTCTCCATG AAAACGACAC CTGGAAGTTG TCGCAATCGC TGAAAGATCG CCCCTTCGTT
    GCGTTAAATC CCACCCGTAA AGCCCAAATG TTGGCCCATC TATGCAACGA CCTGCTCATG
    AACAAGGCCG TGCTCCGCCA GATTGATGGA AGTCTAGAAA CGTGCGCCCA GATGCGAAAG
    GAGAAATACA TGACGGACAT GAAGGTGCGC AAGTACAAGG CTCTCCACAT GCGCAAGGCT
    CGAATTGAGG CCTATGAACG AGCACAGGCG GAGCGTGAGG CGGCTATGCA GGCGTTGATG
    GCACAGCAGA AGCTGGATGC AGAACGTCTT AAGGCGGAGG AGGAAGCCAA GGCCGCGGCG
    GCAGAGGAAG CAGCAGCTGC AGCGGGCACA GATGGAGAGG CAACCAAAGG TGGCTCACCC
    AATGGCGAGA AGCCGGAAGA CGGCGATCAG AATGAAGAGG GAGCCGCCAA GGAACCCCAG
    CAGCAGCAAC AGCAGCCAAT GGAAGTGGAT GGCGTGGTCG ATGAGGCATC TCTTGTAAGT
    CCGGCCAAAA CCATCATTCA AACGGACAAT AGTCTGACGC CCAGTAAACA GGACATGCCC
    ACACCCACCT ACCAAATTAA TGGATCCAGC ACACCTACCA CAAGTGGTGT CACTGGAGGC
    GACATGAATG TTCTGCTGCA GGCCAAGAAA AGCGGGGCCC GAAACTCCAT CAACGATGAA
    CACCACCATG ATGTTAGTAT TATCGACGAT GATCTTTCCG ACTTGGATTC GGAGATCACG
    AATGTCGAGG AGGACGAGGA CAATCGCTTG AGTGCCGATG AGTTGCAAAA GAAGCTCGAC
    AAGATTGTCA GAGCTTCCCT AAACTGCAAG GAGGCACTGG AGAAGAGCAC AAATCAGTTG
    AGAGCTGCGT GCTTTGGGCA GGATCGCTTC TGGCGTCGCT ACTGGAAGTT GCCCAAGGCT
    GGAGGTATTT TCATTGAAGC ACTTGAGTCG GCCCAAAATG ATATTTGCGA TTATCATGAG
    GCATTGGAGG CCATGGATGA CAAAAAGGAT GCAAACGACG AGAAAGAGAA CTCCGAAAAT
    GAGAAGGATG TTGCAGCGGA GTCCAGTGAG CAGCCAATGG AAGTGGATGA GTCCATTACA
    AAACTGGAAG ATGGTGTACC AGCTTCGGAC GTCGGGATGC CTGAAAGTAA TCAACAGAAC
    GCACATCAAG ATGAGGAAGA TGACGATGAT GATGTGACAG AAATCAACAA GGTGGAACCG
    GAAATTGTTG ATTTGGGTGA TGATGACGAT GATGCAGCTC CCCCGCTGCC CAAGATAGAA
    CCTCCCAGGC CAGAGATTAA AGTTAAATCA GAGATGGAGC TGATGGGTCC ACCACCCACG
    ACTATGATAT CCACTAAGAC AGACTTTGAA GCTGAAATTA AAATACCCTC TATGCCTGGC
    ATCTTGATGC CGCCTACCCT CAACAACAAC AACACCAATA ATAACAATAA CAACAATGGA
    AGCGACAACT GTGATAAGTT GGAAACTGGT TTGGGACTAG GACAGCAGCA GCAGAACTTT
    AGCCAGTCTG TGATCAAGAC GGAGGATGTG AAAAAAGAGG ATGATTGCAT AATAGTTTCT
    ACATCCAGTG TTGACGATAC ACCAAAGTGG TTCTCCATTG TCCGGCGGGA GGTTCCATTG
    ATCAGCGAAC TGCCAGCGGA GGAGGGAGGC GTGGTGGGCC AGGAACTTCA GATCAGCTAT
    GCAAATCAGA ACTGCTCCGC TCAGCTGCAG CTGCAAGGTC ATCCGTGGGA TCTGATCAAC
    AACATGCAGT ACTACTCGAT TCCGATGGAC GAATGCAAGG TGGACACCAG CAAGCTGGGC
    AACGAGTGCA TCTTCTCGCT GAGTGGCCTC GATGAAAAGC AAATGCTAGC CAAGGTGGAG
    GAGTACAAGG CCCACAAGGT GGAGTCAAAA AACGGTCTGG GCTCTCCTCA TCGCCACCAC
    GAGACTAAAG ATGATGAGGA GCAGGCCAAG CTGAAGTTGG ACAAGGAGAT TGACACCGAA
    ATGGAAACAG ATGCAGATGA CCTAGCTGGC AAGGAAAAGT TTTTTCGCTT GCGCTCTGAT
    GTACCCCCGG ATACGGGAGG AGGGGTCAGC GAGGGAACCG ATGTAAAGCC CAAGATTGAG
    CTTCGTTTGG ATGAGGCGTT GTCACAGGCA TATTACCACA ACATAGCCAA CATGTCATTG
    AGCAGTGTTC AAACGTATAT ACCCATCGAT ATCCCTCTGC CGCTTTCCAT GACCCCCGAT
    GAGCATCGTC TGCTGGAGCA GGTCAAGCTG GCAGGTTTTC CGGAACGAGT TCACGGAGTT
    TACGTGCCCC GCAGACAGCG CTACGGCTGG TGGCAGTTGG ACGATGAACA GAAGCTGCGC
    CAGCTGCTCA AAACTCTCAA TCCGTCGGGA TTGAGGGAGC GTGAACTGCA GGAGAATCTT
    CAACGATTCC TCGGACTGGA ACAGCCATTG GGTGTGAATT ATAAGCTGAA ATCGGACATC
    GATTTTCCAG AGGAATTCCT AATGCCTGAC AAGAAGGGCG ATTGGAATCC CAAGGTGGCC
    AAACGTGTGG AGCTTGCCCT TATCGAACAA CTAGAATCAC TGGAGGACAA GGTGGCCAGC
    GCTTCTATGC AATTAAAGAA CTGGCAATTG CCCAACCGCG TGGAGAGTGA ACTCACCTTG
    GACTCGCAGG AGGATGTCAC CGAAGAGGAC TTCGTCAGCA TCATACCCAT GATCCGAGAG
    AGAATCATCG ACTTGGAGGC AAATATCGAA AGACGTTACT TGAAGCCGCC TTTGGGCTCG
    CAGACAGGCG ATGCCCATTT GGCGGTTATT GCTCAGAACC AGCATACCAC CACCCAGACG
    CAGAACTCTG CATCGGCAGC AGCATATCTC CTGCAGATGC AACAGCAGCA GCAGCAACAG
    CAGTTGGCCC AGCAGCAGCA GCAACAGCAA CAGGGTTCGG GTGCGGGAAA TAGCCTGAAT
    CCCTCATCCT TCAACGAGCG TACTATGGCA TTGGCGGCGG CAGCAGCTGC TTCGGGGCCA
    GGAAACGCAA CCGGAGTAGC CAACTCGGCA GTCGTGGCAG GAGCCACGCC CTGCGAATCG
    GGCAGCGGAG AACCGAATTC CGGCAACGCG TCACCGGCCA GCAACTGTGA CAGCGATCGG
    GACGAGAAGG TGGAGCAGAT ACCCAAGGGA TTGGTGCAGT GGCGGGACGC AGTATCCCGA
    TCGCACACCA CCGCCCAGCT GGCGATGGCC CTGTACGTTC TGGAATCCTG CGTGGCTTGG
    GACAAGAGCA TTATGAAAGC GTATGCAACA AAAAACAAGT CCAGCAAAAA GAAGAGCAGC
    GCCAAAAAGC AGGCAACTCC CTCGAAGAAA CAGCAGCAGA AGAAAAACAA AAAGGAGCAG
    CAGTTGACCC CCAATGGAAA AGAGAGCAAG AGCAAGAGCG CAATCAATAA GCCAGAAAAC
    CAGGCTCCGC TGAGCATCAA AATCAACCTT AAGGCTCTGG CCCAAAACGG GCAGTGTTTG
    CTGAAAACTA AAACACCACC AATCCTAACC AAATCCCCAA CCGCATCCCC ATCCTCCCAT
    CCAAACACTA GCGACTCCGA CTCGGACTTT GGCAAACGGA CGAAGAAGAA GAGCGGCGGC
    AAACGCAGAC GCTCGGCCAA CAATACAAAC TCTAGCAAAT ATTCCAATTC CTTACAGAAT
    TGCCAGTTCT GCACGTCCGG CGAAAACGAG GACAAGTTGC TACTGTGCGA CGGCTGTGAC
    AAGGGCTATC ACACCTATTG CTTCAAGCCC AAGATGGACA ACATTCCCGA TGGCGATTGG
    TACTGCTACG AGTGCGTGAA CAAGGCCACC AATGAGCGCA AGTGCATCGT TTGCGGCGGT
    CATCGTCCGT CGCCCGTGGG GAAGATGATC TACTGTGACT TGTGTCCTCG TGCCTACCAC
    GCCGATTGCT ATATACCGCC GCTGCTAAAG GTACCGCGTG GCAAGTGGTA CTGCCACGGT
    TGCATCTCCC GGGCGCCTCC GCCGAAGAAA AGAAGTGCAG GTGGAACATC CGGCAGCAGC
    AGCAAATCTA GGAGGGACAG GGATAGGGAG TCGGGCGGAT CGGCCAAGCG GCGTAGCGAC
    AACAGCAAGA CACCAGCCAT GGAGCACATG CAGCAGCAGC AGATGCCACT GGCAGGCGGG
    GATTCCCACC ATCATCATCA TCAGCAGCCG CCGTCGCTGA ACTCCTCGCA CGATGAGTCG
    ATGAATTCTC TGCCGGCGGC TCCTCTGAGT CCGGCGCACT CGGTGGTCTC GGCCACGAAC
    TACGATGACC AGCACCATGC CAACAACTCG GTCGATGGCA GCAGTCGCTT CCACGCGCAT
    CTCATTCCCC CGTCAAATAA TGGCACTGCG GCTCTGCTGG AAGATGTGCC GGGTGGCGCC
    AATGTGATGC CCGGCGTCTA TCCAGTTTAT ACGCCAGTTG CGGCTGGCAA CTTCTCAGCC
    GGATTGATTA ACCAAGCGCC GGTGCAGCCA GCGATGCCGT TTGCCAACGT GGTCGCCATG
    TCGCCACGGG CTGTCACGCC CACCCGCACC CGAACGCCCA CACCGACGCC AGCACCCACT
    CCGCCACCAC CACCGCCGAC ACCGCTGCTA ATGCAGGCCT CGCCCACAGC CACCGCCCTC
    CATGTAAACG CCTGCCAGTC ACCGCCCCAA CAACAGCATG CGCAGCTGAT GACCATGCCC
    TCGCCACCAG CCATTGGAGT CGGAACGGCC ACTAACCAAA TGTCGCCACC GCCCATCAAC
    ATACATGCCA TTCAGGAAGC CAAGGAGAAG CTGAAGCAGG AGAAGAAGGA GAAGCACGCC
    ACCAAGAAGC TCATGAAGGA GCTGGCTGTC TGCAAGACGC TATTGGGGGA AATGGAGCTT
    CATGAGGACT CGTGGCCATT TCTTTTGCCA GTAAACACCA AGCAGTTTCC CACATATCGA
    AAAATAATCA AAACTCCCAT GGACTTGTCC ACAATCAAAA AGAAATTGCA AGATTTGAGC
    TACAAAACCC GTGAGGACTT CTGCGTGGAC GTGCGTCAGA TCTTCGATAA TTGCGAGATG
    TTTAACGAGG ACGATTCGCC CGTTGGCAAG GCAGGCCACG GCATGCGCAA GTTCTTCGAG
    TCCCGCTGGG GTGAACTGAC TGACAAGCAC TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa13280
    Clone ID Related Accession (Same CDS sequence) NM_001273970.1
    Accession Version NM_001273970.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9285bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product toutatis, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAACAAAA ATGCCGGCGA TGGCAGCGAT GGCAAAAACT CAAACAAAAA CTCGAATGCG 
    GGAGGGGGTG GTGGAGCCGG GGGGCCACAC GACCCCACCG GCCTTTTAGA TGCAGCTTCC
    CTGTTCGCTT ACTGGGGACG TGATCCAACT GGAGCGGCGG CTGCAGCGGC TTCCAATCCG
    CTCTTCAACT CGCAGTTCAA TGCCGCCGCT GCTGCCGGAT TGGGTTTATT GCCACAAGCT
    GGAGGCGCCT CTGCCAATGA CCGTTATTCC ATGGCAGCAG CAGCGGCGGC TGCGGCGGGA
    GCCCATCACC ACCAGAACAC GATGGCAGTG GCCGCTTCCC AGGCCGCCAG TTTGGCCGGT
    TTGCATCCAG CAAGCTGGTG GTCAATGGCC CAGTTAGCTG CCCAGGATTA CTTCAGTCGC
    CTGCAAGCCT CGGGTCTTTC CCCCTTTCCA CATCCCGATC TGGCGGCTGC CTTTGGACCA
    GCTGGGATGG GAATGGGCGG CGGTGCTGGT GGAGCGGGTG CTGGAGGTGG TGGAGCAGGT
    GTACTCGGCC AGGGCGGCGG TGGAAATGGC GGAAGTGGCA ACGGTGGTGG TGGATCCTCA
    TCGGGTTCTT CCGGCAGCAA GTCAAACAAG TCGCGCAAGG AGAAGCGAGC CGCCCAGCAG
    CAACAACAGC AACAGCAAAA TCTGGCCAAC AGTCTAAATG CGGCAGCTGC GGCGGCAGCA
    GCAGCAGCAG CCAATAATCC AGCAGCCGCG CTGGCCAGCA TGCATGGTTT TGGTGTAGGA
    GTTCCGGGCA CGAGCATTCC GTCGGTGAGC ACAGGTAGCG GATCAATATC CACAAATAGT
    GGAGGTCATG GAAGTTACAA GAGCACGGCC AGCGCTTATG GTAAGCCCTC GACTATGACG
    AGCAGCAGCA TGGCTAATAA TCCGGGCTCT GCCTACGATC CGGTGACCCT GCACAAGGAG
    CTGCTGGCCA TGCAGGCCGT GGCAGCCGCT GCTTCCGGCT CAGGATCAAG CGGTTCCTCA
    GGCAAGAAGT CCGGTGGGGG TGGCTCCTCA TCCTCCTCGA TGCACAGCCT TGGAATGGGA
    ATTGGTGGCA GCGGTGGCAT GATGTCCAGT GGCAAAGCTT CGACCAGTGT GAGCGCAAGC
    AATTCATCAT TGGGAGGAAT GCACCCCAGT TTGACTAGCA GCAATCCGCT GATGTCGCCC
    CATGCGGGCA TGGGCTCCTC GTCGTCATCA TCGAGCAAGG ATCGTGACAA AAACAATCCC
    TCGCTCAACG CTCTTAACTC GCTCTCACAG TTTGGAGCCC TGGGAATGAC GCCACAGCAG
    AGCATGCAGG CGGCAATGAA TGCTTTTGCA GCCAGCACAG GAGTTTCGCC ATCCGCCACG
    GTAACGTCCT CGCCGCACCA CTCTTCCCAG CAGCAACAGC AAATGGGAGG GAACAGTTCC
    ACGTCGGGAT CGGGCAAGTC CAGTTCCAAG GATTACATGA TGGGTACTGG AAGTGAGCAC
    CCATCTCTGC TTGGAGTTCG TTTACCACCG GACACGGAGA TAATCAAGTA CACGTCCTCG
    ATTGTGGGAC CCAAGATTCC TGGTACTACC TCGCGCGGTC GCAAAAAGAC TATTTCCGAA
    ACAGAACAGC AAACAACACA ACAACAACAG AAACAACAAC ACCAAGCAGA ACAACACCTA
    CTCCAACAAC AGCAACAAGC ACAAAAAGAG CTCGACAGCA CCAAAAATGC CATTTCCTCT
    CTGCTTGCAT TTCCAGGTCT CAGTCCCGCG AAGCGGGCTA GACTCGAAAT GGAGTACGCG
    GCGATGGCTG CGGCCGCTCA ACAGCAGCAT CAGCAGGCAC AGCAGCAACA CCAGGCGCAG
    CAGCAACTCC ACGGGATGCT CGGAGCAGCC GGTATCCCGG GAATGGCTGG ACTGGTCGGT
    CTGCCTGGCA TGAGTGGCAA TCCCCTCGAT CAGTTGAGTG TAAGCAAGGC CAGTTCTTCA
    ACGGCGCCCA CAACCAGTAC CAGTTCCTCC TCAGCAGGGA GCAACCTGCT CAACCAGAGC
    AATAGTGATC GCGTGGAGGT AATCAAACTG CCACCAACAA TTACTTCAAA CGGAGCCTAT
    AATCTCTCAA GTAAGGGAAA AGAGGTGCAC GACCTCACCA CCGACATGGC CACCAACTCT
    GGCGGTGTGA ATCTCAGCCT AAAGTCCAAT GCTGGCTCCA GCGCCTTGAC GCCCAGCGGT
    GCCGTCGGAT CGGCCAGCAA TCCCATTACC ATCGATGACT TTGACGCACC ACTTAATCTT
    TCCATGAAGC CCTCGGATAA GAGCAATAGT AGCTCGTCAA ATGCAGCAGC AGGGGGCTCC
    TCCTCTTCCA GCGCAGCGTT GGCTAACTTG GCGAGCGATT ATCAGGCAGC GTCCAGTGGG
    CAAAGCGGTA ATAGTCTGCA GAGTTTGAGC TCTATTACAG CTGCTTTGGG TGGAACAGGA
    GGTATGCCTG GTGGTTCCAT TTCCGGCAGC GGAGGAACCT CTCCAGCTCC AGCTGGTGCC
    GGATCAGGAG CCACGGGTGG TGGTTCGGGA TCTGGTGGAT CCGGCGGTGG ATCGTCCTCT
    TACAAAGAGG GAAGACCTCG TAACCTGGGA CGCGGCGTAT CCAAGCCCAA GAAGAACACG
    GTAGCTTCGC TGTTGGCTCA ATCCCGAGCT GTGGGCCTTA AACCTATGCT GGCCACGCAA
    CAACTTCTTC AACAGGGTGC TGATATTGAG AAAATCCGCC TGGCACTGAG CGAAGCGAAT
    GCCCATATGG AAACTTCTAC AGATTCCGAA AGCGTGGCAG CCGAAAGTGG TCTTTCGGAG
    TCTGAGAGTG AGGATGCCAA CATTCTGAAT GTAGCGGAGC TAAGGGTGCC ACTTGAATTG
    GGCTGGAAAC GGGAGACGGT GATTCGTGGA CTGACTAAGC AAGGGCAGAT CCGTGGAGAG
    GTCACATATT ATGCACCAGG AAGCACGACG CCGCTAAAGA GCAACGGACA GGTTTTTGCT
    ATCCTGGAGC AGCAGCCATC GAATCTAAGT CGTGAAAACT TTAGTTTTTC CGCGCGAGCC
    ATTGTTGGTT CATTCCTGCA GCCTGCACCA CCGCCGTACG CCAATGATGG CGAGTACATA
    CGGATGACGG ACGAGGACGT GGCCAAGCGG TTAGAGGATC TGAAGGTCTT CACCCGCCAG
    ACTCTTAACG TGGAACAGCG AATTGAGATC GCCAAGCAAC AGCAGGCGAT GCGTGATGCT
    AAGAAGCTGC AAAAGGAAGA ATTGGCCAGA AATAAGGAGA AGGCGCGACA GGAGAAGAAC
    TCCAAGTTGG AGCAGCAGCG CAAGGACAAG GAGCTCAAGA ATCAGCAGGC TGTTGAGGAA
    CTACAGAAAC AAAAAGAGAT GCTGTTGGCT GCCGAAATGG AACGAGAACG TCGCCGCCAA
    CACATGAGCC TCATTCGAAT GTTGGAGCTT CGTCGGAAAT TCGAGGATCG TGAAAAGAAG
    AAACACCAGC TGGTTCTAGA CCGTTTGCTT CTGCGTGAGC GTCGTATGGC GGAGCGAAAA
    CGAGATGCGG AGATTCTGCA GTTGATAAGG CGTCCCAATG AGGACTCCGA AATGCCACAA
    GAATTGGTAA TCCCTGAACT GGATAGGATT GCCGGCAACC GACTTCCCGG CCAGGCAATG
    GCCGATTTGC TCATGGTCTT TGAATTCCTA CATAACTTTG GGGAGACTCT AGGCTTTGAC
    ATGGAATCAC TGCCGTCGCT TCAGAACCTG CACGATGCTT TAATGAGCGA TAGCAACGCG
    GATGCGGAAG AGGAGTTGTT GTCGGTGATG ACGCATCTAT TGGTTTGTGC CATTGAGGAT
    CCAGGTGTTC CCAATCCCGG CAGACACACC ACTTTACTGG GACAATCGCT ACGCAATGCG
    GACATCACCA ACTCGAATGT ATCCGAAATC TTGAGGATTT ATCTGTATGC TACGGCCACA
    GGTGAAGTGC GTCAAATGCA CGGAATCACT GTGGATCGTG AGCGAGAGAG AAGGGTGCCG
    GATCATCATC AACTAGATAG CGATACCACA ACCCACTCAC ATTCGGTAAA GAACCAGGAG
    TACTATAAGC TTCTCCATGA AAACGACACC TGGAAGTTGT CGCAATCGCT GAAAGATCGC
    CCCTTCGTTG CGTTAAATCC CACCCGTAAA GCCCAAATGT TGGCCCATCT ATGCAACGAC
    CTGCTCATGA ACAAGGCCGT GCTCCGCCAG ATTGATGGAA GTCTAGAAAC GTGCGCCCAG
    ATGCGAAAGG AGAAATACAT GACGGACATG AAGGTGCGCA AGTACAAGGC TCTCCACATG
    CGCAAGGCTC GAATTGAGGC CTATGAACGA GCACAGGCGG AGCGTGAGGC GGCTATGCAG
    GCGTTGATGG CACAGCAGAA GCTGGATGCA GAACGTCTTA AGGCGGAGGA GGAAGCCAAG
    GCCGCGGCGG CAGAGGAAGC AGCAGCTGCA GCGGGCACAG ATGGAGAGGC AACCAAAGGT
    GGCTCACCCA ATGGCGAGAA GCCGGAAGAC GGCGATCAGA ATGAAGAGGG AGCCGCCAAG
    GAACCCCAGC AGCAGCAACA GCAGCCAATG GAAGTGGATG GCGTGGTCGA TGAGGCATCT
    CTTGTAAGTC CGGCCAAAAC CATCATTCAA ACGGACAATA GTCTGACGCC CAGTAAACAG
    GACATGCCCA CACCCACCTA CCAAATTAAT GGATCCAGCA CACCTACCAC AAGTGGTGTC
    ACTGGAGGCG ACATGAATGT TCTGCTGCAG GCCAAGAAAA GCGGGGCCCG AAACTCCATC
    AACGATGAAC ACCACCATGA TGTTAGTATT ATCGACGATG ATCTTTCCGA CTTGGATTCG
    GAGATCACGA ATGTCGAGGA GGACGAGGAC AATCGCTTGA GTGCCGATGA GTTGCAAAAG
    AAGCTCGACA AGATTGTCAG AGCTTCCCTA AACTGCAAGG AGGCACTGGA GAAGAGCACA
    AATCAGTTGA GAGCTGCGTG CTTTGGGCAG GATCGCTTCT GGCGTCGCTA CTGGAAGTTG
    CCCAAGGCTG GAGGTATTTT CATTGAAGCA CTTGAGTCGG CCCAAAATGA TATTTGCGAT
    TATCATGAGG CATTGGAGGC CATGGATGAC AAAAAGGATG CAAACGACGA GAAAGAGAAC
    TCCGAAAATG AGAAGGATGT TGCAGCGGAG TCCAGTGAGC AGCCAATGGA AGTGGATGAG
    TCCATTACAA AACTGGAAGA TGGTGTACCA GCTTCGGACG TCGGGATGCC TGAAAGTAAT
    CAACAGAACG CACATCAAGA TGAGGAAGAT GACGATGATG ATGTGACAGA AATCAACAAG
    GTGGAACCGG AAATTGTTGA TTTGGGTGAT GATGACGATG ATGCAGCTCC CCCGCTGCCC
    AAGATAGAAC CTCCCAGGCC AGAGATTAAA GTTAAATCAG AGATGGAGCT GATGGGTCCA
    CCACCCACGA CTATGATATC CACTAAGACA GACTTTGAAG CTGAAATTAA AATACCCTCT
    ATGCCTGGCA TCTTGATGCC GCCTACCCTC AACAACAACA ACACCAATAA TAACAATAAC
    AACAATGGAA GCGACAACTG TGATAAGTTG GAAACTGGTT TGGGACTAGG ACAGCAGCAG
    CAGAACTTTA GCCAGTCTGT GATCAAGACG GAGGATGTGA AAAAAGAGGA TGATTGCATA
    ATAGTTTCTA CATCCAGTGT TGACGATACA CCAAAGTGGT TCTCCATTGT CCGGCGGGAG
    GTTCCATTGA TCAGCGAACT GCCAGCGGAG GAGGGAGGCG TGGTGGGCCA GGAACTTCAG
    ATCAGCTATG CAAATCAGAA CTGCTCCGCT CAGCTGCAGC TGCAAGGTCA TCCGTGGGAT
    CTGATCAACA ACATGCAGTA CTACTCGATT CCGATGGACG AATGCAAGGT GGACACCAGC
    AAGCTGGGCA ACGAGTGCAT CTTCTCGCTG AGTGGCCTCG ATGAAAAGCA AATGCTAGCC
    AAGGTGGAGG AGTACAAGGC CCACAAGGTG GAGTCAAAAA ACGGTCTGGG CTCTCCTCAT
    CGCCACCACG AGACTAAAGA TGATGAGGAG CAGGCCAAGC TGAAGTTGGA CAAGGAGATT
    GACACCGAAA TGGAAACAGA TGCAGATGAC CTAGCTGGCA AGGAAAAGTT TTTTCGCTTG
    CGCTCTGATG TACCCCCGGA TACGGGAGGA GGGGTCAGCG AGGGAACCGA TGTAAAGCCC
    AAGATTGAGC TTCGTTTGGA TGAGGCGTTG TCACAGGCAT ATTACCACAA CATAGCCAAC
    ATGTCATTGA GCAGTGTTCA AACGTATATA CCCATCGATA TCCCTCTGCC GCTTTCCATG
    ACCCCCGATG AGCATCGTCT GCTGGAGCAG GTCAAGCTGG CAGGTTTTCC GGAACGAGTT
    CACGGAGTTT ACGTGCCCCG CAGACAGCGC TACGGCTGGT GGCAGTTGGA CGATGAACAG
    AAGCTGCGCC AGCTGCTCAA AACTCTCAAT CCGTCGGGAT TGAGGGAGCG TGAACTGCAG
    GAGAATCTTC AACGATTCCT CGGACTGGAA CAGCCATTGG GTGTGAATTA TAAGCTGAAA
    TCGGACATCG ATTTTCCAGA GGAATTCCTA ATGCCTGACA AGAAGGGCGA TTGGAATCCC
    AAGGTGGCCA AACGTGTGGA GCTTGCCCTT ATCGAACAAC TAGAATCACT GGAGGACAAG
    GTGGCCAGCG CTTCTATGCA ATTAAAGAAC TGGCAATTGC CCAACCGCGT GGAGAGTGAA
    CTCACCTTGG ACTCGCAGGA GGATGTCACC GAAGAGGACT TCGTCAGCAT CATACCCATG
    ATCCGAGAGA GAATCATCGA CTTGGAGGCA AATATCGAAA GACGTTACTT GAAGCCGCCT
    TTGGGCTCGC AGACAGGCGA TGCCCATTTG GCGGTTATTG CTCAGAACCA GCATACCACC
    ACCCAGACGC AGAACTCTGC ATCGGCAGCA GCATATCTCC TGCAGATGCA ACAGCAGCAG
    CAGCAACAGC AGTTGGCCCA GCAGCAGCAG CAACAGCAAC AGGGTTCGGG TGCGGGAAAT
    AGCCTGAATC CCTCATCCTT CAACGAGCGT ACTATGGCAT TGGCGGCGGC AGCAGCTGCT
    TCGGGGCCAG GAAACGCAAC CGGAGTAGCC AACTCGGCAG TCGTGGCAGG AGCCACGCCC
    TGCGAATCGG GCAGCGGAGA ACCGAATTCC GGCAACGCGT CACCGGCCAG CAACTGTGAC
    AGCGATCGGG ACGAGAAGGT GGAGCAGATA CCCAAGGGAT TGGTGCAGTG GCGGGACGCA
    GTATCCCGAT CGCACACCAC CGCCCAGCTG GCGATGGCCC TGTACGTTCT GGAATCCTGC
    GTGGCTTGGG ACAAGAGCAT TATGAAAGCG TATGCAACAA AAAACAAGTC CAGCAAAAAG
    AAGAGCAGCG CCAAAAAGCA GGCAACTCCC TCGAAGAAAC AGCAGCAGAA GAAAAACAAA
    AAGGAGCAGC AGTTGACCCC CAATGGAAAA GAGAGCAAGA GCAAGAGCGC AATCAATAAG
    CCAGAAAACC AGGCTCCGCT GAGCATCAAA ATCAACCTTA AGGCTCTGGC CCAAAACGGG
    CAGTGTTTGC TGAAAACTAA AACACCACCA ATCCTAACCA AATCCCCAAC CGCATCCCCA
    TCCTCCCATC CAAACACTAG CGACTCCGAC TCGGACTTTG GCAAACGGAC GAAGAAGAAG
    AGCGGCGGCA AACGCAGACG CTCGGCCAAC AATACAAACT CTAGCAAATA TTCCAATTCC
    TTACAGAATT GCCAGTTCTG CACGTCCGGC GAAAACGAGG ACAAGTTGCT ACTGTGCGAC
    GGCTGTGACA AGGGCTATCA CACCTATTGC TTCAAGCCCA AGATGGACAA CATTCCCGAT
    GGCGATTGGT ACTGCTACGA GTGCGTGAAC AAGGCCACCA ATGAGCGCAA GTGCATCGTT
    TGCGGCGGTC ATCGTCCGTC GCCCGTGGGG AAGATGATCT ACTGTGACTT GTGTCCTCGT
    GCCTACCACG CCGATTGCTA TATACCGCCG CTGCTAAAGG TACCGCGTGG CAAGTGGTAC
    TGCCACGGTT GCATCTCCCG GGCGCCTCCG CCGAAGAAAA GAAGTGCAGG TGGAACATCC
    GGCAGCAGCA GCAAATCTAG GAGGGACAGG GATAGGGAGT CGGGCGGATC GGCCAAGCGG
    CGTAGCGACA ACAGCAAGAC ACCAGCCATG GAGCACATGC AGCAGCAGCA GATGCCACTG
    GCAGGCGGGG ATTCCCACCA TCATCATCAT CAGCAGCCGC CGTCGCTGAA CTCCTCGCAC
    GATGAGTCGA TGAATTCTCT GCCGGCGGCT CCTCTGAGTC CGGCGCACTC GGTGGTCTCG
    GCCACGAACT ACGATGACCA GCACCATGCC AACAACTCGG TCGATGGCAG CAGTCGCTTC
    CACGCGCATC TCATTCCCCC GTCAAATAAT GGCACTGCGG CTCTGCTGGA AGATGTGCCG
    GGTGGCGCCA ATGTGATGCC CGGCGTCTAT CCAGTTTATA CGCCAGTTGC GGCTGGCAAC
    TTCTCAGCCG GATTGATTAA CCAAGCGCCG GTGCAGCCAG CGATGCCGTT TGCCAACGTG
    GTCGCCATGT CGCCACGGGC TGTCACGCCC ACCCGCACCC GAACGCCCAC ACCGACGCCA
    GCACCCACTC CGCCACCACC ACCGCCGACA CCGCTGCTAA TGCAGGCCTC GCCCACAGCC
    ACCGCCCTCC ATGTAAACGC CTGCCAGTCA CCGCCCCAAC AACAGCATGC GCAGCTGATG
    ACCATGCCCT CGCCACCAGC CATTGGAGTC GGAACGGCCA CTAACCAAAT GTCGCCACCG
    CCCATCAACA TACATGCCAT TCAGGAAGCC AAGGAGAAGC TGAAGCAGGA GAAGAAGGAG
    AAGCACGCCA CCAAGAAGCT CATGAAGGAG CTGGCTGTCT GCAAGACGCT ATTGGGGGAA
    ATGGAGCTTC ATGAGGACTC GTGGCCATTT CTTTTGCCAG TAAACACCAA GCAGTTTCCC
    ACATATCGAA AAATAATCAA AACTCCCATG GACTTGTCCA CAATCAAAAA GAAATTGCAA
    GATTTGAGCT ACAAAACCCG TGAGGACTTC TGCGTGGACG TGCGTCAGAT CTTCGATAAT
    TGCGAGATGT TTAACGAGGA CGATTCGCCC GTTGGCAAGG CAGGCCACGG CATGCGCAAG
    TTCTTCGAGT CCCGCTGGGG TGAACTGACT GACAAGCACT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260899.1
    CDS490..9774
    Translation

    Target ORF information:

    RefSeq Version NM_001273970.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster toutatis (tou), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273970.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAACAAAA ATGCCGGCGA TGGCAGCGAT GGCAAAAACT CAAACAAAAA CTCGAATGCG 
    GGAGGGGGTG GTGGAGCCGG GGGGCCACAC GACCCCACCG GCCTTTTAGA TGCAGCTTCC
    CTGTTCGCTT ACTGGGGACG TGATCCAACT GGAGCGGCGG CTGCAGCGGC TTCCAATCCG
    CTCTTCAACT CGCAGTTCAA TGCCGCCGCT GCTGCCGGAT TGGGTTTATT GCCACAAGCT
    GGAGGCGCCT CTGCCAATGA CCGTTATTCC ATGGCAGCAG CAGCGGCGGC TGCGGCGGGA
    GCCCATCACC ACCAGAACAC GATGGCAGTG GCCGCTTCCC AGGCCGCCAG TTTGGCCGGT
    TTGCATCCAG CAAGCTGGTG GTCAATGGCC CAGTTAGCTG CCCAGGATTA CTTCAGTCGC
    CTGCAAGCCT CGGGTCTTTC CCCCTTTCCA CATCCCGATC TGGCGGCTGC CTTTGGACCA
    GCTGGGATGG GAATGGGCGG CGGTGCTGGT GGAGCGGGTG CTGGAGGTGG TGGAGCAGGT
    GTACTCGGCC AGGGCGGCGG TGGAAATGGC GGAAGTGGCA ACGGTGGTGG TGGATCCTCA
    TCGGGTTCTT CCGGCAGCAA GTCAAACAAG TCGCGCAAGG AGAAGCGAGC CGCCCAGCAG
    CAACAACAGC AACAGCAAAA TCTGGCCAAC AGTCTAAATG CGGCAGCTGC GGCGGCAGCA
    GCAGCAGCAG CCAATAATCC AGCAGCCGCG CTGGCCAGCA TGCATGGTTT TGGTGTAGGA
    GTTCCGGGCA CGAGCATTCC GTCGGTGAGC ACAGGTAGCG GATCAATATC CACAAATAGT
    GGAGGTCATG GAAGTTACAA GAGCACGGCC AGCGCTTATG GTAAGCCCTC GACTATGACG
    AGCAGCAGCA TGGCTAATAA TCCGGGCTCT GCCTACGATC CGGTGACCCT GCACAAGGAG
    CTGCTGGCCA TGCAGGCCGT GGCAGCCGCT GCTTCCGGCT CAGGATCAAG CGGTTCCTCA
    GGCAAGAAGT CCGGTGGGGG TGGCTCCTCA TCCTCCTCGA TGCACAGCCT TGGAATGGGA
    ATTGGTGGCA GCGGTGGCAT GATGTCCAGT GGCAAAGCTT CGACCAGTGT GAGCGCAAGC
    AATTCATCAT TGGGAGGAAT GCACCCCAGT TTGACTAGCA GCAATCCGCT GATGTCGCCC
    CATGCGGGCA TGGGCTCCTC GTCGTCATCA TCGAGCAAGG ATCGTGACAA AAACAATCCC
    TCGCTCAACG CTCTTAACTC GCTCTCACAG TTTGGAGCCC TGGGAATGAC GCCACAGCAG
    AGCATGCAGG CGGCAATGAA TGCTTTTGCA GCCAGCACAG GAGTTTCGCC ATCCGCCACG
    GTAACGTCCT CGCCGCACCA CTCTTCCCAG CAGCAACAGC AAATGGGAGG GAACAGTTCC
    ACGTCGGGAT CGGGCAAGTC CAGTTCCAAG GATTACATGA TGGGTACTGG AAGTGAGCAC
    CCATCTCTGC TTGGAGTTCG TTTACCACCG GACACGGAGA TAATCAAGTA CACGTCCTCG
    ATTGTGGGAC CCAAGATTCC TGGTACTACC TCGCGCGGTC GCAAAAAGAC TATTTCCGAA
    ACAGAACAGC AAACAACACA ACAACAACAG AAACAACAAC ACCAAGCAGA ACAACACCTA
    CTCCAACAAC AGCAACAAGC ACAAAAAGAG CTCGACAGCA CCAAAAATGC CATTTCCTCT
    CTGCTTGCAT TTCCAGGTCT CAGTCCCGCG AAGCGGGCTA GACTCGAAAT GGAGTACGCG
    GCGATGGCTG CGGCCGCTCA ACAGCAGCAT CAGCAGGCAC AGCAGCAACA CCAGGCGCAG
    CAGCAACTCC ACGGGATGCT CGGAGCAGCC GGTATCCCGG GAATGGCTGG ACTGGTCGGT
    CTGCCTGGCA TGAGTGGCAA TCCCCTCGAT CAGTTGAGTG TAAGCAAGGC CAGTTCTTCA
    ACGGCGCCCA CAACCAGTAC CAGTTCCTCC TCAGCAGGGA GCAACCTGCT CAACCAGAGC
    AATAGTGATC GCGTGGAGGT AATCAAACTG CCACCAACAA TTACTTCAAA CGGAGCCTAT
    AATCTCTCAA GTAAGGGAAA AGAGGTGCAC GACCTCACCA CCGACATGGC CACCAACTCT
    GGCGGTGTGA ATCTCAGCCT AAAGTCCAAT GCTGGCTCCA GCGCCTTGAC GCCCAGCGGT
    GCCGTCGGAT CGGCCAGCAA TCCCATTACC ATCGATGACT TTGACGCACC ACTTAATCTT
    TCCATGAAGC CCTCGGATAA GAGCAATAGT AGCTCGTCAA ATGCAGCAGC AGGGGGCTCC
    TCCTCTTCCA GCGCAGCGTT GGCTAACTTG GCGAGCGATT ATCAGGCAGC GTCCAGTGGG
    CAAAGCGGTA ATAGTCTGCA GAGTTTGAGC TCTATTACAG CTGCTTTGGG TGGAACAGGA
    GGTATGCCTG GTGGTTCCAT TTCCGGCAGC GGAGGAACCT CTCCAGCTCC AGCTGGTGCC
    GGATCAGGAG CCACGGGTGG TGGTTCGGGA TCTGGTGGAT CCGGCGGTGG ATCGTCCTCT
    TACAAAGAGG GAAGACCTCG TAACCTGGGA CGCGGCGTAT CCAAGCCCAA GAAGAACACG
    GTAGCTTCGC TGTTGGCTCA ATCCCGAGCT GTGGGCCTTA AACCTATGCT GGCCACGCAA
    CAACTTCTTC AACAGGGTGC TGATATTGAG AAAATCCGCC TGGCACTGAG CGAAGCGAAT
    GCCCATATGG AAACTTCTAC AGATTCCGAA AGCGTGGCAG CCGAAAGTGG TCTTTCGGAG
    TCTGAGAGTG AGGATGCCAA CATTCTGAAT GTAGCGGAGC TAAGGGTGCC ACTTGAATTG
    GGCTGGAAAC GGGAGACGGT GATTCGTGGA CTGACTAAGC AAGGGCAGAT CCGTGGAGAG
    GTCACATATT ATGCACCAGG AAGCACGACG CCGCTAAAGA GCAACGGACA GGTTTTTGCT
    ATCCTGGAGC AGCAGCCATC GAATCTAAGT CGTGAAAACT TTAGTTTTTC CGCGCGAGCC
    ATTGTTGGTT CATTCCTGCA GCCTGCACCA CCGCCGTACG CCAATGATGG CGAGTACATA
    CGGATGACGG ACGAGGACGT GGCCAAGCGG TTAGAGGATC TGAAGGTCTT CACCCGCCAG
    ACTCTTAACG TGGAACAGCG AATTGAGATC GCCAAGCAAC AGCAGGCGAT GCGTGATGCT
    AAGAAGCTGC AAAAGGAAGA ATTGGCCAGA AATAAGGAGA AGGCGCGACA GGAGAAGAAC
    TCCAAGTTGG AGCAGCAGCG CAAGGACAAG GAGCTCAAGA ATCAGCAGGC TGTTGAGGAA
    CTACAGAAAC AAAAAGAGAT GCTGTTGGCT GCCGAAATGG AACGAGAACG TCGCCGCCAA
    CACATGAGCC TCATTCGAAT GTTGGAGCTT CGTCGGAAAT TCGAGGATCG TGAAAAGAAG
    AAACACCAGC TGGTTCTAGA CCGTTTGCTT CTGCGTGAGC GTCGTATGGC GGAGCGAAAA
    CGAGATGCGG AGATTCTGCA GTTGATAAGG CGTCCCAATG AGGACTCCGA AATGCCACAA
    GAATTGGTAA TCCCTGAACT GGATAGGATT GCCGGCAACC GACTTCCCGG CCAGGCAATG
    GCCGATTTGC TCATGGTCTT TGAATTCCTA CATAACTTTG GGGAGACTCT AGGCTTTGAC
    ATGGAATCAC TGCCGTCGCT TCAGAACCTG CACGATGCTT TAATGAGCGA TAGCAACGCG
    GATGCGGAAG AGGAGTTGTT GTCGGTGATG ACGCATCTAT TGGTTTGTGC CATTGAGGAT
    CCAGGTGTTC CCAATCCCGG CAGACACACC ACTTTACTGG GACAATCGCT ACGCAATGCG
    GACATCACCA ACTCGAATGT ATCCGAAATC TTGAGGATTT ATCTGTATGC TACGGCCACA
    GGTGAAGTGC GTCAAATGCA CGGAATCACT GTGGATCGTG AGCGAGAGAG AAGGGTGCCG
    GATCATCATC AACTAGATAG CGATACCACA ACCCACTCAC ATTCGGTAAA GAACCAGGAG
    TACTATAAGC TTCTCCATGA AAACGACACC TGGAAGTTGT CGCAATCGCT GAAAGATCGC
    CCCTTCGTTG CGTTAAATCC CACCCGTAAA GCCCAAATGT TGGCCCATCT ATGCAACGAC
    CTGCTCATGA ACAAGGCCGT GCTCCGCCAG ATTGATGGAA GTCTAGAAAC GTGCGCCCAG
    ATGCGAAAGG AGAAATACAT GACGGACATG AAGGTGCGCA AGTACAAGGC TCTCCACATG
    CGCAAGGCTC GAATTGAGGC CTATGAACGA GCACAGGCGG AGCGTGAGGC GGCTATGCAG
    GCGTTGATGG CACAGCAGAA GCTGGATGCA GAACGTCTTA AGGCGGAGGA GGAAGCCAAG
    GCCGCGGCGG CAGAGGAAGC AGCAGCTGCA GCGGGCACAG ATGGAGAGGC AACCAAAGGT
    GGCTCACCCA ATGGCGAGAA GCCGGAAGAC GGCGATCAGA ATGAAGAGGG AGCCGCCAAG
    GAACCCCAGC AGCAGCAACA GCAGCCAATG GAAGTGGATG GCGTGGTCGA TGAGGCATCT
    CTTGTAAGTC CGGCCAAAAC CATCATTCAA ACGGACAATA GTCTGACGCC CAGTAAACAG
    GACATGCCCA CACCCACCTA CCAAATTAAT GGATCCAGCA CACCTACCAC AAGTGGTGTC
    ACTGGAGGCG ACATGAATGT TCTGCTGCAG GCCAAGAAAA GCGGGGCCCG AAACTCCATC
    AACGATGAAC ACCACCATGA TGTTAGTATT ATCGACGATG ATCTTTCCGA CTTGGATTCG
    GAGATCACGA ATGTCGAGGA GGACGAGGAC AATCGCTTGA GTGCCGATGA GTTGCAAAAG
    AAGCTCGACA AGATTGTCAG AGCTTCCCTA AACTGCAAGG AGGCACTGGA GAAGAGCACA
    AATCAGTTGA GAGCTGCGTG CTTTGGGCAG GATCGCTTCT GGCGTCGCTA CTGGAAGTTG
    CCCAAGGCTG GAGGTATTTT CATTGAAGCA CTTGAGTCGG CCCAAAATGA TATTTGCGAT
    TATCATGAGG CATTGGAGGC CATGGATGAC AAAAAGGATG CAAACGACGA GAAAGAGAAC
    TCCGAAAATG AGAAGGATGT TGCAGCGGAG TCCAGTGAGC AGCCAATGGA AGTGGATGAG
    TCCATTACAA AACTGGAAGA TGGTGTACCA GCTTCGGACG TCGGGATGCC TGAAAGTAAT
    CAACAGAACG CACATCAAGA TGAGGAAGAT GACGATGATG ATGTGACAGA AATCAACAAG
    GTGGAACCGG AAATTGTTGA TTTGGGTGAT GATGACGATG ATGCAGCTCC CCCGCTGCCC
    AAGATAGAAC CTCCCAGGCC AGAGATTAAA GTTAAATCAG AGATGGAGCT GATGGGTCCA
    CCACCCACGA CTATGATATC CACTAAGACA GACTTTGAAG CTGAAATTAA AATACCCTCT
    ATGCCTGGCA TCTTGATGCC GCCTACCCTC AACAACAACA ACACCAATAA TAACAATAAC
    AACAATGGAA GCGACAACTG TGATAAGTTG GAAACTGGTT TGGGACTAGG ACAGCAGCAG
    CAGAACTTTA GCCAGTCTGT GATCAAGACG GAGGATGTGA AAAAAGAGGA TGATTGCATA
    ATAGTTTCTA CATCCAGTGT TGACGATACA CCAAAGTGGT TCTCCATTGT CCGGCGGGAG
    GTTCCATTGA TCAGCGAACT GCCAGCGGAG GAGGGAGGCG TGGTGGGCCA GGAACTTCAG
    ATCAGCTATG CAAATCAGAA CTGCTCCGCT CAGCTGCAGC TGCAAGGTCA TCCGTGGGAT
    CTGATCAACA ACATGCAGTA CTACTCGATT CCGATGGACG AATGCAAGGT GGACACCAGC
    AAGCTGGGCA ACGAGTGCAT CTTCTCGCTG AGTGGCCTCG ATGAAAAGCA AATGCTAGCC
    AAGGTGGAGG AGTACAAGGC CCACAAGGTG GAGTCAAAAA ACGGTCTGGG CTCTCCTCAT
    CGCCACCACG AGACTAAAGA TGATGAGGAG CAGGCCAAGC TGAAGTTGGA CAAGGAGATT
    GACACCGAAA TGGAAACAGA TGCAGATGAC CTAGCTGGCA AGGAAAAGTT TTTTCGCTTG
    CGCTCTGATG TACCCCCGGA TACGGGAGGA GGGGTCAGCG AGGGAACCGA TGTAAAGCCC
    AAGATTGAGC TTCGTTTGGA TGAGGCGTTG TCACAGGCAT ATTACCACAA CATAGCCAAC
    ATGTCATTGA GCAGTGTTCA AACGTATATA CCCATCGATA TCCCTCTGCC GCTTTCCATG
    ACCCCCGATG AGCATCGTCT GCTGGAGCAG GTCAAGCTGG CAGGTTTTCC GGAACGAGTT
    CACGGAGTTT ACGTGCCCCG CAGACAGCGC TACGGCTGGT GGCAGTTGGA CGATGAACAG
    AAGCTGCGCC AGCTGCTCAA AACTCTCAAT CCGTCGGGAT TGAGGGAGCG TGAACTGCAG
    GAGAATCTTC AACGATTCCT CGGACTGGAA CAGCCATTGG GTGTGAATTA TAAGCTGAAA
    TCGGACATCG ATTTTCCAGA GGAATTCCTA ATGCCTGACA AGAAGGGCGA TTGGAATCCC
    AAGGTGGCCA AACGTGTGGA GCTTGCCCTT ATCGAACAAC TAGAATCACT GGAGGACAAG
    GTGGCCAGCG CTTCTATGCA ATTAAAGAAC TGGCAATTGC CCAACCGCGT GGAGAGTGAA
    CTCACCTTGG ACTCGCAGGA GGATGTCACC GAAGAGGACT TCGTCAGCAT CATACCCATG
    ATCCGAGAGA GAATCATCGA CTTGGAGGCA AATATCGAAA GACGTTACTT GAAGCCGCCT
    TTGGGCTCGC AGACAGGCGA TGCCCATTTG GCGGTTATTG CTCAGAACCA GCATACCACC
    ACCCAGACGC AGAACTCTGC ATCGGCAGCA GCATATCTCC TGCAGATGCA ACAGCAGCAG
    CAGCAACAGC AGTTGGCCCA GCAGCAGCAG CAACAGCAAC AGGGTTCGGG TGCGGGAAAT
    AGCCTGAATC CCTCATCCTT CAACGAGCGT ACTATGGCAT TGGCGGCGGC AGCAGCTGCT
    TCGGGGCCAG GAAACGCAAC CGGAGTAGCC AACTCGGCAG TCGTGGCAGG AGCCACGCCC
    TGCGAATCGG GCAGCGGAGA ACCGAATTCC GGCAACGCGT CACCGGCCAG CAACTGTGAC
    AGCGATCGGG ACGAGAAGGT GGAGCAGATA CCCAAGGGAT TGGTGCAGTG GCGGGACGCA
    GTATCCCGAT CGCACACCAC CGCCCAGCTG GCGATGGCCC TGTACGTTCT GGAATCCTGC
    GTGGCTTGGG ACAAGAGCAT TATGAAAGCG TATGCAACAA AAAACAAGTC CAGCAAAAAG
    AAGAGCAGCG CCAAAAAGCA GGCAACTCCC TCGAAGAAAC AGCAGCAGAA GAAAAACAAA
    AAGGAGCAGC AGTTGACCCC CAATGGAAAA GAGAGCAAGA GCAAGAGCGC AATCAATAAG
    CCAGAAAACC AGGCTCCGCT GAGCATCAAA ATCAACCTTA AGGCTCTGGC CCAAAACGGG
    CAGTGTTTGC TGAAAACTAA AACACCACCA ATCCTAACCA AATCCCCAAC CGCATCCCCA
    TCCTCCCATC CAAACACTAG CGACTCCGAC TCGGACTTTG GCAAACGGAC GAAGAAGAAG
    AGCGGCGGCA AACGCAGACG CTCGGCCAAC AATACAAACT CTAGCAAATA TTCCAATTCC
    TTACAGAATT GCCAGTTCTG CACGTCCGGC GAAAACGAGG ACAAGTTGCT ACTGTGCGAC
    GGCTGTGACA AGGGCTATCA CACCTATTGC TTCAAGCCCA AGATGGACAA CATTCCCGAT
    GGCGATTGGT ACTGCTACGA GTGCGTGAAC AAGGCCACCA ATGAGCGCAA GTGCATCGTT
    TGCGGCGGTC ATCGTCCGTC GCCCGTGGGG AAGATGATCT ACTGTGACTT GTGTCCTCGT
    GCCTACCACG CCGATTGCTA TATACCGCCG CTGCTAAAGG TACCGCGTGG CAAGTGGTAC
    TGCCACGGTT GCATCTCCCG GGCGCCTCCG CCGAAGAAAA GAAGTGCAGG TGGAACATCC
    GGCAGCAGCA GCAAATCTAG GAGGGACAGG GATAGGGAGT CGGGCGGATC GGCCAAGCGG
    CGTAGCGACA ACAGCAAGAC ACCAGCCATG GAGCACATGC AGCAGCAGCA GATGCCACTG
    GCAGGCGGGG ATTCCCACCA TCATCATCAT CAGCAGCCGC CGTCGCTGAA CTCCTCGCAC
    GATGAGTCGA TGAATTCTCT GCCGGCGGCT CCTCTGAGTC CGGCGCACTC GGTGGTCTCG
    GCCACGAACT ACGATGACCA GCACCATGCC AACAACTCGG TCGATGGCAG CAGTCGCTTC
    CACGCGCATC TCATTCCCCC GTCAAATAAT GGCACTGCGG CTCTGCTGGA AGATGTGCCG
    GGTGGCGCCA ATGTGATGCC CGGCGTCTAT CCAGTTTATA CGCCAGTTGC GGCTGGCAAC
    TTCTCAGCCG GATTGATTAA CCAAGCGCCG GTGCAGCCAG CGATGCCGTT TGCCAACGTG
    GTCGCCATGT CGCCACGGGC TGTCACGCCC ACCCGCACCC GAACGCCCAC ACCGACGCCA
    GCACCCACTC CGCCACCACC ACCGCCGACA CCGCTGCTAA TGCAGGCCTC GCCCACAGCC
    ACCGCCCTCC ATGTAAACGC CTGCCAGTCA CCGCCCCAAC AACAGCATGC GCAGCTGATG
    ACCATGCCCT CGCCACCAGC CATTGGAGTC GGAACGGCCA CTAACCAAAT GTCGCCACCG
    CCCATCAACA TACATGCCAT TCAGGAAGCC AAGGAGAAGC TGAAGCAGGA GAAGAAGGAG
    AAGCACGCCA CCAAGAAGCT CATGAAGGAG CTGGCTGTCT GCAAGACGCT ATTGGGGGAA
    ATGGAGCTTC ATGAGGACTC GTGGCCATTT CTTTTGCCAG TAAACACCAA GCAGTTTCCC
    ACATATCGAA AAATAATCAA AACTCCCATG GACTTGTCCA CAATCAAAAA GAAATTGCAA
    GATTTGAGCT ACAAAACCCG TGAGGACTTC TGCGTGGACG TGCGTCAGAT CTTCGATAAT
    TGCGAGATGT TTAACGAGGA CGATTCGCCC GTTGGCAAGG CAGGCCACGG CATGCGCAAG
    TTCTTCGAGT CCCGCTGGGG TGAACTGACT GACAAGCACT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa13287
    Clone ID Related Accession (Same CDS sequence) NM_001273969.1
    Accession Version NM_001273969.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9177bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product toutatis, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGAACAAAA ATGCCGGCGA TGGCAGCGAT GGCAAAAACT CAAACAAAAA CTCGAATGCG 
    GGAGGGGGTG GTGGAGCCGG GGGGCCACAC GACCCCACCG GCCTTTTAGA TGCAGCTTCC
    CTGTTCGCTT ACTGGGGACG TGATCCAACT GGAGCGGCGG CTGCAGCGGC TTCCAATCCG
    CTCTTCAACT CGCAGTTCAA TGCCGCCGCT GCTGCCGGAT TGGGTTTATT GCCACAAGCT
    GGAGGCGCCT CTGCCAATGA CCGTTATTCC ATGGCAGCAG CAGCGGCGGC TGCGGCGGGA
    GCCCATCACC ACCAGAACAC GATGGCAGTG GCCGCTTCCC AGGCCGCCAG TTTGGCCGGT
    TTGCATCCAG CAAGCTGGTG GTCAATGGCC CAGTTAGCTG CCCAGGATTA CTTCAGTCGC
    CTGCAAGCCT CGGGTCTTTC CCCCTTTCCA CATCCCGATC TGGCGGCTGC CTTTGGACCA
    GCTGGGATGG GAATGGGCGG CGGTGCTGGT GGAGCGGGTG CTGGAGGTGG TGGAGCAGGT
    GTACTCGGCC AGGGCGGCGG TGGAAATGGC GGAAGTGGCA ACGGTGGTGG TGGATCCTCA
    TCGGGTTCTT CCGGCAGCAA GTCAAACAAG TCGCGCAAGG AGAAGCGAGC CGCCCAGCAG
    CAACAACAGC AACAGCAAAA TCTGGCCAAC AGTCTAAATG CGGCAGCTGC GGCGGCAGCA
    GCAGCAGCAG CCAATAATCC AGCAGCCGCG CTGGCCAGCA TGCATGGTTT TGGTGTAGGA
    GTTCCGGGCA CGAGCATTCC GTCGGTGAGC ACAGGTAGCG GATCAATATC CACAAATAGT
    GGAGGTCATG GAAGTTACAA GAGCACGGCC AGCGCTTATG GTAAGCCCTC GACTATGACG
    AGCAGCAGCA TGGCTAATAA TCCGGGCTCT GCCTACGATC CGGTGACCCT GCACAAGGAG
    CTGCTGGCCA TGCAGGCCGT GGCAGCCGCT GCTTCCGGCT CAGGATCAAG CGGTTCCTCA
    GGCAAGAAGT CCGGTGGGGG TGGCTCCTCA TCCTCCTCGA TGCACAGCCT TGGAATGGGA
    ATTGGTGGCA GCGGTGGCAT GATGTCCAGT GGCAAAGCTT CGACCAGTGT GAGCGCAAGC
    AATTCATCAT TGGGAGGAAT GCACCCCAGT TTGACTAGCA GCAATCCGCT GATGTCGCCC
    CATGCGGGCA TGGGCTCCTC GTCGTCATCA TCGAGCAAGG ATCGTGACAA AAACAATCCC
    TCGCTCAACG CTCTTAACTC GCTCTCACAG TTTGGAGCCC TGGGAATGAC GCCACAGCAG
    AGCATGCAGG CGGCAATGAA TGCTTTTGCA GCCAGCACAG GAGTTTCGCC ATCCGCCACG
    GTAACGTCCT CGCCGCACCA CTCTTCCCAG CAGCAACAGC AAATGGGAGG GAACAGTTCC
    ACGTCGGGAT CGGGCAAGTC CAGTTCCAAG GATTACATGA TGGGTACTGG AAGTGAGCAC
    CCATCTCTGC TTGGAGTTCG TTTACCACCG GACACGGAGA TAATCAAGTA CACGTCCTCG
    ATTGTGGGAC CCAAGATTCC TGGTCTCAGT CCCGCGAAGC GGGCTAGACT CGAAATGGAG
    TACGCGGCGA TGGCTGCGGC CGCTCAACAG CAGCATCAGC AGGCACAGCA GCAACACCAG
    GCGCAGCAGC AACTCCACGG GATGCTCGGA GCAGCCGGTA TCCCGGGAAT GGCTGGACTG
    GTCGGTCTGC CTGGCATGAG TGGCAATCCC CTCGATCAGT TGAGTGTAAG CAAGGCCAGT
    TCTTCAACGG CGCCCACAAC CAGTACCAGT TCCTCCTCAG CAGGGAGCAA CCTGCTCAAC
    CAGAGCAATA GTGATCGCGT GGAGGTAATC AAACTGCCAC CAACAATTAC TTCAAACGGA
    GCCTATAATC TCTCAAGTAA GGGAAAAGAG GTGCACGACC TCACCACCGA CATGGCCACC
    AACTCTGGCG GTGTGAATCT CAGCCTAAAG TCCAATGCTG GCTCCAGCGC CTTGACGCCC
    AGCGGTGCCG TCGGATCGGC CAGCAATCCC ATTACCATCG ATGACTTTGA CGCACCACTT
    AATCTTTCCA TGAAGCCCTC GGATAAGAGC AATAGTAGCT CGTCAAATGC AGCAGCAGGG
    GGCTCCTCCT CTTCCAGCGC AGCGTTGGCT AACTTGGCGA GCGATTATCA GGCAGCGTCC
    AGTGGGCAAA GCGGTAATAG TCTGCAGAGT TTGAGCTCTA TTACAGCTGC TTTGGGTGGA
    ACAGGAGGTA TGCCTGGTGG TTCCATTTCC GGCAGCGGAG GAACCTCTCC AGCTCCAGCT
    GGTGCCGGAT CAGGAGCCAC GGGTGGTGGT TCGGGATCTG GTGGATCCGG CGGTGGATCG
    TCCTCTTACA AAGAGGGAAG ACCTCGTAAC CTGGGACGCG GCGTATCCAA GCCCAAGAAG
    AACACGGTAG CTTCGCTGTT GGCTCAATCC CGAGCTGTGG GCCTTAAACC TATGCTGGCC
    ACGCAACAAC TTCTTCAACA GGGTGCTGAT ATTGAGAAAA TCCGCCTGGC ACTGAGCGAA
    GCGAATGCCC ATATGGAAAC TTCTACAGAT TCCGAAAGCG TGGCAGCCGA AAGTGGTCTT
    TCGGAGTCTG AGAGTGAGGA TGCCAACATT CTGAATGTAG CGGAGCTAAG GGTGCCACTT
    GAATTGGGCT GGAAACGGGA GACGGTGATT CGTGGACTGA CTAAGCAAGG GCAGATCCGT
    GGAGAGGTCA CATATTATGC ACCAGGAAGC ACGACGCCGC TAAAGAGCAA CGGACAGGTT
    TTTGCTATCC TGGAGCAGCA GCCATCGAAT CTAAGTCGTG AAAACTTTAG TTTTTCCGCG
    CGAGCCATTG TTGGTTCATT CCTGCAGCCT GCACCACCGC CGTACGCCAA TGATGGCGAG
    TACATACGGA TGACGGACGA GGACGTGGCC AAGCGGTTAG AGGATCTGAA GGTCTTCACC
    CGCCAGACTC TTAACGTGGA ACAGCGAATT GAGATCGCCA AGCAACAGCA GGCGATGCGT
    GATGCTAAGA AGCTGCAAAA GGAAGAATTG GCCAGAAATA AGGAGAAGGC GCGACAGGAG
    AAGAACTCCA AGTTGGAGCA GCAGCGCAAG GACAAGGAGC TCAAGAATCA GCAGGCTGTT
    GAGGAGCGCA AAAAGCGTCA GGAGGAGCTA GATCGCCTTA AGCAAGAGGA ATTGCTTAAG
    AAGCAACAGG AACTACAGAA ACAAAAAGAG ATGCTGTTGG CTGCCGAAAT GGAACGAGAA
    CGTCGCCGCC AACACATGAG CCTCATTCGA ATGTTGGAGC TTCGTCGGAA ATTCGAGGAT
    CGTGAAAAGA AGAAACACCA GCTGGTTCTA GACCGTTTGC TTCTGCGTGA GCGTCGTATG
    GCGGAGCGAA AACGAGATGC GGAGATTCTG CAGTTGATAA GGCGTCCCAA TGAGGACTCC
    GAAATGCCAC AAGAATTGGT AATCCCTGAA CTGGATAGGA TTGCCGGCAA CCGACTTCCC
    GGCCAGGCAA TGGCCGATTT GCTCATGGTC TTTGAATTCC TACATAACTT TGGGGAGACT
    CTAGGCTTTG ACATGGAATC ACTGCCGTCG CTTCAGAACC TGCACGATGC TTTAATGAGC
    GATAGCAACG CGGATGCGGA AGAGGAGTTG TTGTCGGTGA TGACGCATCT ATTGGTTTGT
    GCCATTGAGG ATCCAGGTGT TCCCAATCCC GGCAGACACA CCACTTTACT GGGACAATCG
    CTACGCAATG CGGACATCAC CAACTCGAAT GTATCCGAAA TCTTGAGGAT TTATCTGTAT
    GCTACGGCCA CAGGTGAAGT GCGTCAAATG CACGGAATCA CTGTGGATCG TGAGCGAGAG
    AGAAGGGTGC CGGATCATCA TCAACTAGAT AGCGATACCA CAACCCACTC ACATTCGGTA
    AAGAACCAGG AGTACTATAA GCTTCTCCAT GAAAACGACA CCTGGAAGTT GTCGCAATCG
    CTGAAAGATC GCCCCTTCGT TGCGTTAAAT CCCACCCGTA AAGCCCAAAT GTTGGCCCAT
    CTATGCAACG ACCTGCTCAT GAACAAGGCC GTGCTCCGCC AGATTGATGG AAGTCTAGAA
    ACGTGCGCCC AGATGCGAAA GGAGAAATAC ATGACGGACA TGAAGGTGCG CAAGTACAAG
    GCTCTCCACA TGCGCAAGGC TCGAATTGAG GCCTATGAAC GAGCACAGGC GGAGCGTGAG
    GCGGCTATGC AGGCGTTGAT GGCACAGCAG AAGCTGGATG CAGAACGTCT TAAGGCGGAG
    GAGGAAGCCA AGGCCGCGGC GGCAGAGGAA GCAGCAGCTG CAGCGGGCAC AGATGGAGAG
    GCAACCAAAG GTGGCTCACC CAATGGCGAG AAGCCGGAAG ACGGCGATCA GAATGAAGAG
    GGAGCCGCCA AGGAACCCCA GCAGCAGCAA CAGCAGCCAA TGGAAGTGGA TGGCGTGGTC
    GATGAGGCAT CTCTTGTAAG TCCGGCCAAA ACCATCATTC AAACGGACAA TAGTCTGACG
    CCCAGTAAAC AGGACATGCC CACACCCACC TACCAAATTA ATGGATCCAG CACACCTACC
    ACAAGTGGTG TCACTGGAGG CGACATGAAT GTTCTGCTGC AGGCCAAGAA AAGCGGGGCC
    CGAAACTCCA TCAACGATGA ACACCACCAT GATGTTAGTA TTATCGACGA TGATCTTTCC
    GACTTGGATT CGGAGATCAC GAATGTCGAG GAGGACGAGG ACAATCGCTT GAGTGCCGAT
    GAGTTGCAAA AGAAGCTCGA CAAGATTGTC AGAGCTTCCC TAAACTGCAA GGAGGCACTG
    GAGAAGAGCA CAAATCAGTT GAGAGCTGCG TGCTTTGGGC AGGATCGCTT CTGGCGTCGC
    TACTGGAAGT TGCCCAAGGC TGGAGGTATT TTCATTGAAG CACTTGAGTC GGCCCAAAAT
    GATATTTGCG ATTATCATGA GGCATTGGAG GCCATGGATG ACAAAAAGGA TGCAAACGAC
    GAGAAAGAGA ACTCCGAAAA TGAGAAGGAT GTTGCAGCGG AGTCCAGTGA GCAGCCAATG
    GAAGTGGATG AGTCCATTAC AAAACTGGAA GATGGTGTAC CAGCTTCGGA CGTCGGGATG
    CCTGAAAGTA ATCAACAGAA CGCACATCAA GATGAGGAAG ATGACGATGA TGATGTGACA
    GAAATCAACA AGGTGGAACC GGAAATTGTT GATTTGGGTG ATGATGACGA TGATGCAGCT
    CCCCCGCTGC CCAAGATAGA ACCTCCCAGG CCAGAGATTA AAGTTAAATC AGAGATGGAG
    CTGATGGGTC CACCACCCAC GACTATGATA TCCACTAAGA CAGACTTTGA AGCTGAAATT
    AAAATACCCT CTATGCCTGG CATCTTGATG CCGCCTACCC TCAACAACAA CAACACCAAT
    AATAACAATA ACAACAATGG AAGCGACAAC TGTGATAAGT TGGAAACTGG TTTGGGACTA
    GGACAGCAGC AGCAGAACTT TAGCCAGTCT GTGATCAAGA CGGAGGATGT GAAAAAAGAG
    GATGATTGCA TAATAGTTTC TACATCCAGT GTTGACGATA CACCAAAGTG GTTCTCCATT
    GTCCGGCGGG AGGTTCCATT GATCAGCGAA CTGCCAGCGG AGGAGGGAGG CGTGGTGGGC
    CAGGAACTTC AGATCAGCTA TGCAAATCAG AACTGCTCCG CTCAGCTGCA GCTGCAAGGT
    CATCCGTGGG ATCTGATCAA CAACATGCAG TACTACTCGA TTCCGATGGA CGAATGCAAG
    GTGGACACCA GCAAGCTGGG CAACGAGTGC ATCTTCTCGC TGAGTGGCCT CGATGAAAAG
    CAAATGCTAG CCAAGGTGGA GGAGTACAAG GCCCACAAGG TGGAGTCAAA AAACGGTCTG
    GGCTCTCCTC ATCGCCACCA CGAGACTAAA GATGATGAGG AGCAGGCCAA GCTGAAGTTG
    GACAAGGAGA TTGACACCGA AATGGAAACA GATGCAGATG ACCTAGCTGG CAAGGAAAAG
    TTTTTTCGCT TGCGCTCTGA TGTACCCCCG GATACGGGAG GAGGGGTCAG CGAGGGAACC
    GATGTAAAGC CCAAGATTGA GCTTCGTTTG GATGAGGCGT TGTCACAGGC ATATTACCAC
    AACATAGCCA ACATGTCATT GAGCAGTGTT CAAACGTATA TACCCATCGA TATCCCTCTG
    CCGCTTTCCA TGACCCCCGA TGAGCATCGT CTGCTGGAGC AGGTCAAGCT GGCAGGTTTT
    CCGGAACGAG TTCACGGAGT TTACGTGCCC CGCAGACAGC GCTACGGCTG GTGGCAGTTG
    GACGATGAAC AGAAGCTGCG CCAGCTGCTC AAAACTCTCA ATCCGTCGGG ATTGAGGGAG
    CGTGAACTGC AGGAGAATCT TCAACGATTC CTCGGACTGG AACAGCCATT GGGTGTGAAT
    TATAAGCTGA AATCGGACAT CGATTTTCCA GAGGAATTCC TAATGCCTGA CAAGAAGGGC
    GATTGGAATC CCAAGGTGGC CAAACGTGTG GAGCTTGCCC TTATCGAACA ACTAGAATCA
    CTGGAGGACA AGGTGGCCAG CGCTTCTATG CAATTAAAGA ACTGGCAATT GCCCAACCGC
    GTGGAGAGTG AACTCACCTT GGACTCGCAG GAGGATGTCA CCGAAGAGGA CTTCGTCAGC
    ATCATACCCA TGATCCGAGA GAGAATCATC GACTTGGAGG CAAATATCGA AAGACGTTAC
    TTGAAGCCGC CTTTGGGCTC GCAGACAGGC GATGCCCATT TGGCGGTTAT TGCTCAGAAC
    CAGCATACCA CCACCCAGAC GCAGAACTCT GCATCGGCAG CAGCATATCT CCTGCAGATG
    CAACAGCAGC AGCAGCAACA GCAGTTGGCC CAGCAGCAGC AGCAACAGCA ACAGGGTTCG
    GGTGCGGGAA ATAGCCTGAA TCCCTCATCC TTCAACGAGC GTACTATGGC ATTGGCGGCG
    GCAGCAGCTG CTTCGGGGCC AGGAAACGCA ACCGGAGTAG CCAACTCGGC AGTCGTGGCA
    GGAGCCACGC CCTGCGAATC GGGCAGCGGA GAACCGAATT CCGGCAACGC GTCACCGGCC
    AGCAACTGTG ACAGCGATCG GGACGAGAAG GTGGAGCAGA TACCCAAGGG ATTGGTGCAG
    TGGCGGGACG CAGTATCCCG ATCGCACACC ACCGCCCAGC TGGCGATGGC CCTGTACGTT
    CTGGAATCCT GCGTGGCTTG GGACAAGAGC ATTATGAAAG CGTATGCAAC AAAAAACAAG
    TCCAGCAAAA AGAAGAGCAG CGCCAAAAAG CAGGCAACTC CCTCGAAGAA ACAGCAGCAG
    AAGAAAAACA AAAAGGAGCA GCAGTTGACC CCCAATGGAA AAGAGAGCAA GAGCAAGAGC
    GCAATCAATA AGCCAGAAAA CCAGGCTCCG CTGAGCATCA AAATCAACCT TAAGGCTCTG
    GCCCAAAACG GGCAGTGTTT GCTGAAAACT AAAACACCAC CAATCCTAAC CAAATCCCCA
    ACCGCATCCC CATCCTCCCA TCCAAACACT AGCGACTCCG ACTCGGACTT TGGCAAACGG
    ACGAAGAAGA AGAGCGGCGG CAAACGCAGA CGCTCGGCCA ACAATACAAA CTCTAGCAAA
    TATTCCAATT CCTTACAGAA TTGCCAGTTC TGCACGTCCG GCGAAAACGA GGACAAGTTG
    CTACTGTGCG ACGGCTGTGA CAAGGGCTAT CACACCTATT GCTTCAAGCC CAAGATGGAC
    AACATTCCCG ATGGCGATTG GTACTGCTAC GAGTGCGTGA ACAAGGCCAC CAATGAGCGC
    AAGTGCATCG TTTGCGGCGG TCATCGTCCG TCGCCCGTGG GGAAGATGAT CTACTGTGAC
    TTGTGTCCTC GTGCCTACCA CGCCGATTGC TATATACCGC CGCTGCTAAA GGTACCGCGT
    GGCAAGTGGT ACTGCCACGG TTGCATCTCC CGGGCGCCTC CGCCGAAGAA AAGAAGTGCA
    GGTGGAACAT CCGGCAGCAG CAGCAAATCT AGGAGGGACA GGGATAGGGA GTCGGGCGGA
    TCGGCCAAGC GGCGTAGCGA CAACAGCAAG ACACCAGCCA TGGAGCACAT GCAGCAGCAG
    CAGATGCCAC TGGCAGGCGG GGATTCCCAC CATCATCATC ATCAGCAGCC GCCGTCGCTG
    AACTCCTCGC ACGATGAGTC GATGAATTCT CTGCCGGCGG CTCCTCTGAG TCCGGCGCAC
    TCGGTGGTCT CGGCCACGAA CTACGATGAC CAGCACCATG CCAACAACTC GGTCGATGGC
    AGCAGTCGCT TCCACGCGCA TCTCATTCCC CCGTCAAATA ATGGCACTGC GGCTCTGCTG
    GAAGATGTGC CGGGTGGCGC CAATGTGATG CCCGGCGTCT ATCCAGTTTA TACGCCAGTT
    GCGGCTGGCA ACTTCTCAGC CGGATTGATT AACCAAGCGC CGGTGCAGCC AGCGATGCCG
    TTTGCCAACG TGGTCGCCAT GTCGCCACGG GCTGTCACGC CCACCCGCAC CCGAACGCCC
    ACACCGACGC CAGCACCCAC TCCGCCACCA CCACCGCCGA CACCGCTGCT AATGCAGGCC
    TCGCCCACAG CCACCGCCCT CCATGTAAAC GCCTGCCAGT CACCGCCCCA ACAACAGCAT
    GCGCAGCTGA TGACCATGCC CTCGCCACCA GCCATTGGAG TCGGAACGGC CACTAACCAA
    ATGTCGCCAC CGCCCATCAA CATACATGCC ATTCAGGAAG CCAAGGAGAA GCTGAAGCAG
    GAGAAGAAGG AGAAGCACGC CACCAAGAAG CTCATGAAGG AGCTGGCTGT CTGCAAGACG
    CTATTGGGGG AAATGGAGCT TCATGAGGAC TCGTGGCCAT TTCTTTTGCC AGTAAACACC
    AAGCAGTTTC CCACATATCG AAAAATAATC AAAACTCCCA TGGACTTGTC CACAATCAAA
    AAGAAATTGC AAGATTTGAG CTACAAAACC CGTGAGGACT TCTGCGTGGA CGTGCGTCAG
    ATCTTCGATA ATTGCGAGAT GTTTAACGAG GACGATTCGC CCGTTGGCAA GGCAGGCCAC
    GGCATGCGCA AGTTCTTCGA GTCCCGCTGG GGTGAACTGA CTGACAAGCA CTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260898.1
    CDS225..9401
    Translation

    Target ORF information:

    RefSeq Version NM_001273969.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster toutatis (tou), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273969.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGAACAAAA ATGCCGGCGA TGGCAGCGAT GGCAAAAACT CAAACAAAAA CTCGAATGCG 
    GGAGGGGGTG GTGGAGCCGG GGGGCCACAC GACCCCACCG GCCTTTTAGA TGCAGCTTCC
    CTGTTCGCTT ACTGGGGACG TGATCCAACT GGAGCGGCGG CTGCAGCGGC TTCCAATCCG
    CTCTTCAACT CGCAGTTCAA TGCCGCCGCT GCTGCCGGAT TGGGTTTATT GCCACAAGCT
    GGAGGCGCCT CTGCCAATGA CCGTTATTCC ATGGCAGCAG CAGCGGCGGC TGCGGCGGGA
    GCCCATCACC ACCAGAACAC GATGGCAGTG GCCGCTTCCC AGGCCGCCAG TTTGGCCGGT
    TTGCATCCAG CAAGCTGGTG GTCAATGGCC CAGTTAGCTG CCCAGGATTA CTTCAGTCGC
    CTGCAAGCCT CGGGTCTTTC CCCCTTTCCA CATCCCGATC TGGCGGCTGC CTTTGGACCA
    GCTGGGATGG GAATGGGCGG CGGTGCTGGT GGAGCGGGTG CTGGAGGTGG TGGAGCAGGT
    GTACTCGGCC AGGGCGGCGG TGGAAATGGC GGAAGTGGCA ACGGTGGTGG TGGATCCTCA
    TCGGGTTCTT CCGGCAGCAA GTCAAACAAG TCGCGCAAGG AGAAGCGAGC CGCCCAGCAG
    CAACAACAGC AACAGCAAAA TCTGGCCAAC AGTCTAAATG CGGCAGCTGC GGCGGCAGCA
    GCAGCAGCAG CCAATAATCC AGCAGCCGCG CTGGCCAGCA TGCATGGTTT TGGTGTAGGA
    GTTCCGGGCA CGAGCATTCC GTCGGTGAGC ACAGGTAGCG GATCAATATC CACAAATAGT
    GGAGGTCATG GAAGTTACAA GAGCACGGCC AGCGCTTATG GTAAGCCCTC GACTATGACG
    AGCAGCAGCA TGGCTAATAA TCCGGGCTCT GCCTACGATC CGGTGACCCT GCACAAGGAG
    CTGCTGGCCA TGCAGGCCGT GGCAGCCGCT GCTTCCGGCT CAGGATCAAG CGGTTCCTCA
    GGCAAGAAGT CCGGTGGGGG TGGCTCCTCA TCCTCCTCGA TGCACAGCCT TGGAATGGGA
    ATTGGTGGCA GCGGTGGCAT GATGTCCAGT GGCAAAGCTT CGACCAGTGT GAGCGCAAGC
    AATTCATCAT TGGGAGGAAT GCACCCCAGT TTGACTAGCA GCAATCCGCT GATGTCGCCC
    CATGCGGGCA TGGGCTCCTC GTCGTCATCA TCGAGCAAGG ATCGTGACAA AAACAATCCC
    TCGCTCAACG CTCTTAACTC GCTCTCACAG TTTGGAGCCC TGGGAATGAC GCCACAGCAG
    AGCATGCAGG CGGCAATGAA TGCTTTTGCA GCCAGCACAG GAGTTTCGCC ATCCGCCACG
    GTAACGTCCT CGCCGCACCA CTCTTCCCAG CAGCAACAGC AAATGGGAGG GAACAGTTCC
    ACGTCGGGAT CGGGCAAGTC CAGTTCCAAG GATTACATGA TGGGTACTGG AAGTGAGCAC
    CCATCTCTGC TTGGAGTTCG TTTACCACCG GACACGGAGA TAATCAAGTA CACGTCCTCG
    ATTGTGGGAC CCAAGATTCC TGGTCTCAGT CCCGCGAAGC GGGCTAGACT CGAAATGGAG
    TACGCGGCGA TGGCTGCGGC CGCTCAACAG CAGCATCAGC AGGCACAGCA GCAACACCAG
    GCGCAGCAGC AACTCCACGG GATGCTCGGA GCAGCCGGTA TCCCGGGAAT GGCTGGACTG
    GTCGGTCTGC CTGGCATGAG TGGCAATCCC CTCGATCAGT TGAGTGTAAG CAAGGCCAGT
    TCTTCAACGG CGCCCACAAC CAGTACCAGT TCCTCCTCAG CAGGGAGCAA CCTGCTCAAC
    CAGAGCAATA GTGATCGCGT GGAGGTAATC AAACTGCCAC CAACAATTAC TTCAAACGGA
    GCCTATAATC TCTCAAGTAA GGGAAAAGAG GTGCACGACC TCACCACCGA CATGGCCACC
    AACTCTGGCG GTGTGAATCT CAGCCTAAAG TCCAATGCTG GCTCCAGCGC CTTGACGCCC
    AGCGGTGCCG TCGGATCGGC CAGCAATCCC ATTACCATCG ATGACTTTGA CGCACCACTT
    AATCTTTCCA TGAAGCCCTC GGATAAGAGC AATAGTAGCT CGTCAAATGC AGCAGCAGGG
    GGCTCCTCCT CTTCCAGCGC AGCGTTGGCT AACTTGGCGA GCGATTATCA GGCAGCGTCC
    AGTGGGCAAA GCGGTAATAG TCTGCAGAGT TTGAGCTCTA TTACAGCTGC TTTGGGTGGA
    ACAGGAGGTA TGCCTGGTGG TTCCATTTCC GGCAGCGGAG GAACCTCTCC AGCTCCAGCT
    GGTGCCGGAT CAGGAGCCAC GGGTGGTGGT TCGGGATCTG GTGGATCCGG CGGTGGATCG
    TCCTCTTACA AAGAGGGAAG ACCTCGTAAC CTGGGACGCG GCGTATCCAA GCCCAAGAAG
    AACACGGTAG CTTCGCTGTT GGCTCAATCC CGAGCTGTGG GCCTTAAACC TATGCTGGCC
    ACGCAACAAC TTCTTCAACA GGGTGCTGAT ATTGAGAAAA TCCGCCTGGC ACTGAGCGAA
    GCGAATGCCC ATATGGAAAC TTCTACAGAT TCCGAAAGCG TGGCAGCCGA AAGTGGTCTT
    TCGGAGTCTG AGAGTGAGGA TGCCAACATT CTGAATGTAG CGGAGCTAAG GGTGCCACTT
    GAATTGGGCT GGAAACGGGA GACGGTGATT CGTGGACTGA CTAAGCAAGG GCAGATCCGT
    GGAGAGGTCA CATATTATGC ACCAGGAAGC ACGACGCCGC TAAAGAGCAA CGGACAGGTT
    TTTGCTATCC TGGAGCAGCA GCCATCGAAT CTAAGTCGTG AAAACTTTAG TTTTTCCGCG
    CGAGCCATTG TTGGTTCATT CCTGCAGCCT GCACCACCGC CGTACGCCAA TGATGGCGAG
    TACATACGGA TGACGGACGA GGACGTGGCC AAGCGGTTAG AGGATCTGAA GGTCTTCACC
    CGCCAGACTC TTAACGTGGA ACAGCGAATT GAGATCGCCA AGCAACAGCA GGCGATGCGT
    GATGCTAAGA AGCTGCAAAA GGAAGAATTG GCCAGAAATA AGGAGAAGGC GCGACAGGAG
    AAGAACTCCA AGTTGGAGCA GCAGCGCAAG GACAAGGAGC TCAAGAATCA GCAGGCTGTT
    GAGGAGCGCA AAAAGCGTCA GGAGGAGCTA GATCGCCTTA AGCAAGAGGA ATTGCTTAAG
    AAGCAACAGG AACTACAGAA ACAAAAAGAG ATGCTGTTGG CTGCCGAAAT GGAACGAGAA
    CGTCGCCGCC AACACATGAG CCTCATTCGA ATGTTGGAGC TTCGTCGGAA ATTCGAGGAT
    CGTGAAAAGA AGAAACACCA GCTGGTTCTA GACCGTTTGC TTCTGCGTGA GCGTCGTATG
    GCGGAGCGAA AACGAGATGC GGAGATTCTG CAGTTGATAA GGCGTCCCAA TGAGGACTCC
    GAAATGCCAC AAGAATTGGT AATCCCTGAA CTGGATAGGA TTGCCGGCAA CCGACTTCCC
    GGCCAGGCAA TGGCCGATTT GCTCATGGTC TTTGAATTCC TACATAACTT TGGGGAGACT
    CTAGGCTTTG ACATGGAATC ACTGCCGTCG CTTCAGAACC TGCACGATGC TTTAATGAGC
    GATAGCAACG CGGATGCGGA AGAGGAGTTG TTGTCGGTGA TGACGCATCT ATTGGTTTGT
    GCCATTGAGG ATCCAGGTGT TCCCAATCCC GGCAGACACA CCACTTTACT GGGACAATCG
    CTACGCAATG CGGACATCAC CAACTCGAAT GTATCCGAAA TCTTGAGGAT TTATCTGTAT
    GCTACGGCCA CAGGTGAAGT GCGTCAAATG CACGGAATCA CTGTGGATCG TGAGCGAGAG
    AGAAGGGTGC CGGATCATCA TCAACTAGAT AGCGATACCA CAACCCACTC ACATTCGGTA
    AAGAACCAGG AGTACTATAA GCTTCTCCAT GAAAACGACA CCTGGAAGTT GTCGCAATCG
    CTGAAAGATC GCCCCTTCGT TGCGTTAAAT CCCACCCGTA AAGCCCAAAT GTTGGCCCAT
    CTATGCAACG ACCTGCTCAT GAACAAGGCC GTGCTCCGCC AGATTGATGG AAGTCTAGAA
    ACGTGCGCCC AGATGCGAAA GGAGAAATAC ATGACGGACA TGAAGGTGCG CAAGTACAAG
    GCTCTCCACA TGCGCAAGGC TCGAATTGAG GCCTATGAAC GAGCACAGGC GGAGCGTGAG
    GCGGCTATGC AGGCGTTGAT GGCACAGCAG AAGCTGGATG CAGAACGTCT TAAGGCGGAG
    GAGGAAGCCA AGGCCGCGGC GGCAGAGGAA GCAGCAGCTG CAGCGGGCAC AGATGGAGAG
    GCAACCAAAG GTGGCTCACC CAATGGCGAG AAGCCGGAAG ACGGCGATCA GAATGAAGAG
    GGAGCCGCCA AGGAACCCCA GCAGCAGCAA CAGCAGCCAA TGGAAGTGGA TGGCGTGGTC
    GATGAGGCAT CTCTTGTAAG TCCGGCCAAA ACCATCATTC AAACGGACAA TAGTCTGACG
    CCCAGTAAAC AGGACATGCC CACACCCACC TACCAAATTA ATGGATCCAG CACACCTACC
    ACAAGTGGTG TCACTGGAGG CGACATGAAT GTTCTGCTGC AGGCCAAGAA AAGCGGGGCC
    CGAAACTCCA TCAACGATGA ACACCACCAT GATGTTAGTA TTATCGACGA TGATCTTTCC
    GACTTGGATT CGGAGATCAC GAATGTCGAG GAGGACGAGG ACAATCGCTT GAGTGCCGAT
    GAGTTGCAAA AGAAGCTCGA CAAGATTGTC AGAGCTTCCC TAAACTGCAA GGAGGCACTG
    GAGAAGAGCA CAAATCAGTT GAGAGCTGCG TGCTTTGGGC AGGATCGCTT CTGGCGTCGC
    TACTGGAAGT TGCCCAAGGC TGGAGGTATT TTCATTGAAG CACTTGAGTC GGCCCAAAAT
    GATATTTGCG ATTATCATGA GGCATTGGAG GCCATGGATG ACAAAAAGGA TGCAAACGAC
    GAGAAAGAGA ACTCCGAAAA TGAGAAGGAT GTTGCAGCGG AGTCCAGTGA GCAGCCAATG
    GAAGTGGATG AGTCCATTAC AAAACTGGAA GATGGTGTAC CAGCTTCGGA CGTCGGGATG
    CCTGAAAGTA ATCAACAGAA CGCACATCAA GATGAGGAAG ATGACGATGA TGATGTGACA
    GAAATCAACA AGGTGGAACC GGAAATTGTT GATTTGGGTG ATGATGACGA TGATGCAGCT
    CCCCCGCTGC CCAAGATAGA ACCTCCCAGG CCAGAGATTA AAGTTAAATC AGAGATGGAG
    CTGATGGGTC CACCACCCAC GACTATGATA TCCACTAAGA CAGACTTTGA AGCTGAAATT
    AAAATACCCT CTATGCCTGG CATCTTGATG CCGCCTACCC TCAACAACAA CAACACCAAT
    AATAACAATA ACAACAATGG AAGCGACAAC TGTGATAAGT TGGAAACTGG TTTGGGACTA
    GGACAGCAGC AGCAGAACTT TAGCCAGTCT GTGATCAAGA CGGAGGATGT GAAAAAAGAG
    GATGATTGCA TAATAGTTTC TACATCCAGT GTTGACGATA CACCAAAGTG GTTCTCCATT
    GTCCGGCGGG AGGTTCCATT GATCAGCGAA CTGCCAGCGG AGGAGGGAGG CGTGGTGGGC
    CAGGAACTTC AGATCAGCTA TGCAAATCAG AACTGCTCCG CTCAGCTGCA GCTGCAAGGT
    CATCCGTGGG ATCTGATCAA CAACATGCAG TACTACTCGA TTCCGATGGA CGAATGCAAG
    GTGGACACCA GCAAGCTGGG CAACGAGTGC ATCTTCTCGC TGAGTGGCCT CGATGAAAAG
    CAAATGCTAG CCAAGGTGGA GGAGTACAAG GCCCACAAGG TGGAGTCAAA AAACGGTCTG
    GGCTCTCCTC ATCGCCACCA CGAGACTAAA GATGATGAGG AGCAGGCCAA GCTGAAGTTG
    GACAAGGAGA TTGACACCGA AATGGAAACA GATGCAGATG ACCTAGCTGG CAAGGAAAAG
    TTTTTTCGCT TGCGCTCTGA TGTACCCCCG GATACGGGAG GAGGGGTCAG CGAGGGAACC
    GATGTAAAGC CCAAGATTGA GCTTCGTTTG GATGAGGCGT TGTCACAGGC ATATTACCAC
    AACATAGCCA ACATGTCATT GAGCAGTGTT CAAACGTATA TACCCATCGA TATCCCTCTG
    CCGCTTTCCA TGACCCCCGA TGAGCATCGT CTGCTGGAGC AGGTCAAGCT GGCAGGTTTT
    CCGGAACGAG TTCACGGAGT TTACGTGCCC CGCAGACAGC GCTACGGCTG GTGGCAGTTG
    GACGATGAAC AGAAGCTGCG CCAGCTGCTC AAAACTCTCA ATCCGTCGGG ATTGAGGGAG
    CGTGAACTGC AGGAGAATCT TCAACGATTC CTCGGACTGG AACAGCCATT GGGTGTGAAT
    TATAAGCTGA AATCGGACAT CGATTTTCCA GAGGAATTCC TAATGCCTGA CAAGAAGGGC
    GATTGGAATC CCAAGGTGGC CAAACGTGTG GAGCTTGCCC TTATCGAACA ACTAGAATCA
    CTGGAGGACA AGGTGGCCAG CGCTTCTATG CAATTAAAGA ACTGGCAATT GCCCAACCGC
    GTGGAGAGTG AACTCACCTT GGACTCGCAG GAGGATGTCA CCGAAGAGGA CTTCGTCAGC
    ATCATACCCA TGATCCGAGA GAGAATCATC GACTTGGAGG CAAATATCGA AAGACGTTAC
    TTGAAGCCGC CTTTGGGCTC GCAGACAGGC GATGCCCATT TGGCGGTTAT TGCTCAGAAC
    CAGCATACCA CCACCCAGAC GCAGAACTCT GCATCGGCAG CAGCATATCT CCTGCAGATG
    CAACAGCAGC AGCAGCAACA GCAGTTGGCC CAGCAGCAGC AGCAACAGCA ACAGGGTTCG
    GGTGCGGGAA ATAGCCTGAA TCCCTCATCC TTCAACGAGC GTACTATGGC ATTGGCGGCG
    GCAGCAGCTG CTTCGGGGCC AGGAAACGCA ACCGGAGTAG CCAACTCGGC AGTCGTGGCA
    GGAGCCACGC CCTGCGAATC GGGCAGCGGA GAACCGAATT CCGGCAACGC GTCACCGGCC
    AGCAACTGTG ACAGCGATCG GGACGAGAAG GTGGAGCAGA TACCCAAGGG ATTGGTGCAG
    TGGCGGGACG CAGTATCCCG ATCGCACACC ACCGCCCAGC TGGCGATGGC CCTGTACGTT
    CTGGAATCCT GCGTGGCTTG GGACAAGAGC ATTATGAAAG CGTATGCAAC AAAAAACAAG
    TCCAGCAAAA AGAAGAGCAG CGCCAAAAAG CAGGCAACTC CCTCGAAGAA ACAGCAGCAG
    AAGAAAAACA AAAAGGAGCA GCAGTTGACC CCCAATGGAA AAGAGAGCAA GAGCAAGAGC
    GCAATCAATA AGCCAGAAAA CCAGGCTCCG CTGAGCATCA AAATCAACCT TAAGGCTCTG
    GCCCAAAACG GGCAGTGTTT GCTGAAAACT AAAACACCAC CAATCCTAAC CAAATCCCCA
    ACCGCATCCC CATCCTCCCA TCCAAACACT AGCGACTCCG ACTCGGACTT TGGCAAACGG
    ACGAAGAAGA AGAGCGGCGG CAAACGCAGA CGCTCGGCCA ACAATACAAA CTCTAGCAAA
    TATTCCAATT CCTTACAGAA TTGCCAGTTC TGCACGTCCG GCGAAAACGA GGACAAGTTG
    CTACTGTGCG ACGGCTGTGA CAAGGGCTAT CACACCTATT GCTTCAAGCC CAAGATGGAC
    AACATTCCCG ATGGCGATTG GTACTGCTAC GAGTGCGTGA ACAAGGCCAC CAATGAGCGC
    AAGTGCATCG TTTGCGGCGG TCATCGTCCG TCGCCCGTGG GGAAGATGAT CTACTGTGAC
    TTGTGTCCTC GTGCCTACCA CGCCGATTGC TATATACCGC CGCTGCTAAA GGTACCGCGT
    GGCAAGTGGT ACTGCCACGG TTGCATCTCC CGGGCGCCTC CGCCGAAGAA AAGAAGTGCA
    GGTGGAACAT CCGGCAGCAG CAGCAAATCT AGGAGGGACA GGGATAGGGA GTCGGGCGGA
    TCGGCCAAGC GGCGTAGCGA CAACAGCAAG ACACCAGCCA TGGAGCACAT GCAGCAGCAG
    CAGATGCCAC TGGCAGGCGG GGATTCCCAC CATCATCATC ATCAGCAGCC GCCGTCGCTG
    AACTCCTCGC ACGATGAGTC GATGAATTCT CTGCCGGCGG CTCCTCTGAG TCCGGCGCAC
    TCGGTGGTCT CGGCCACGAA CTACGATGAC CAGCACCATG CCAACAACTC GGTCGATGGC
    AGCAGTCGCT TCCACGCGCA TCTCATTCCC CCGTCAAATA ATGGCACTGC GGCTCTGCTG
    GAAGATGTGC CGGGTGGCGC CAATGTGATG CCCGGCGTCT ATCCAGTTTA TACGCCAGTT
    GCGGCTGGCA ACTTCTCAGC CGGATTGATT AACCAAGCGC CGGTGCAGCC AGCGATGCCG
    TTTGCCAACG TGGTCGCCAT GTCGCCACGG GCTGTCACGC CCACCCGCAC CCGAACGCCC
    ACACCGACGC CAGCACCCAC TCCGCCACCA CCACCGCCGA CACCGCTGCT AATGCAGGCC
    TCGCCCACAG CCACCGCCCT CCATGTAAAC GCCTGCCAGT CACCGCCCCA ACAACAGCAT
    GCGCAGCTGA TGACCATGCC CTCGCCACCA GCCATTGGAG TCGGAACGGC CACTAACCAA
    ATGTCGCCAC CGCCCATCAA CATACATGCC ATTCAGGAAG CCAAGGAGAA GCTGAAGCAG
    GAGAAGAAGG AGAAGCACGC CACCAAGAAG CTCATGAAGG AGCTGGCTGT CTGCAAGACG
    CTATTGGGGG AAATGGAGCT TCATGAGGAC TCGTGGCCAT TTCTTTTGCC AGTAAACACC
    AAGCAGTTTC CCACATATCG AAAAATAATC AAAACTCCCA TGGACTTGTC CACAATCAAA
    AAGAAATTGC AAGATTTGAG CTACAAAACC CGTGAGGACT TCTGCGTGGA CGTGCGTCAG
    ATCTTCGATA ATTGCGAGAT GTTTAACGAG GACGATTCGC CCGTTGGCAA GGCAGGCCAC
    GGCATGCGCA AGTTCTTCGA GTCCCGCTGG GGTGAACTGA CTGACAAGCA CTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.