THG cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	THG
Entrez Gene ID	34876
Full Name	tRNA-histidine guanylyltransferase
Synonyms	BG:DS00929.4,C1,CG4103,Dmel\CG4103,br4,l(2)35Bc,l(2)br,l(2)br11,l(2)br13,l(2)br4,l(2)k08808,tRNA-guanylyltransferase
General protein information	Preferred Names tRNA-histidine guanylyltransferase Names CG4103 gene product from transcript CG4103-RB CG4103-PA CG4103-PB THG-PA THG-PB lethal (2) 35Bc lethal 11 in the black-reduced region lethal 13 in the black-reduced region lethal 4 in the black-reduced region lethal group C1 tRNA guanylyltransferase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35B8-35B8

mRNA and Protein(s)

mRNA	Protein	Name
NM_135893.2	NP_609737.1	tRNA-histidine guanylyltransferase, isoform A
NM_001201886.1	NP_001188815.1	tRNA-histidine guanylyltransferase, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	THG1L	XP_518066.2
Bos taurus (cattle)	THG1L	NP_001073702.1
Mus musculus (house mouse)	Thg1l	NP_001074438.1
Danio rerio (zebrafish)	thg1l	NP_001007456.1
Saccharomyces cerevisiae (baker's yeast)	THG1	NP_011538.3
Neurospora crassa	NCU02105	XP_964099.2
Arabidopsis thaliana (thale cress)	AT2G31580	NP_565727.2
Xenopus tropicalis (tropical clawed frog)	LOC100487878	XP_004912832.1
	THG1L	XP_001113603.1
Canis lupus familiaris (dog)	THG1L	XP_536453.2
Eremothecium gossypii	AGOS_ABR031C	NP_982977.1
Xenopus tropicalis (tropical clawed frog)	LOC101733043	XP_004920397.1
Gallus gallus (chicken)	THG1L	NP_001025787.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011752	XP_320758.4
Homo sapiens (human)	THG1L	NP_060342.2
Drosophila melanogaster (fruit fly)	l(2)35Bc	NP_001188815.1
Kluyveromyces lactis	KLLA0B06237g	XP_451814.1
Magnaporthe oryzae (rice blast fungus)	MGG_14742	XP_003713640.1
Arabidopsis thaliana (thale cress)	AT2G32320	NP_001154545.1
Oryza sativa (rice)	Os05g0535500	NP_001056156.1
Rattus norvegicus (Norway rat)	Thg1l	NP_001013988.1
Schizosaccharomyces pombe (fission yeast)	SPCC63.07	NP_587981.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following THG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14700	NM_135893.2 Latest version!	Drosophila melanogaster tRNA-histidine guanylyltransferase (THG), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa14700	NM_001201886.1 Latest version!	Drosophila melanogaster tRNA-histidine guanylyltransferase (THG), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THG ( NM_135893.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609737.1 Drosophila melanogaster tRNA-histidine guanylyltransferase (THG), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14700
Clone ID Related Accession (Same CDS sequence)	NM_135893.2 , NM_001201886.1
Accession Version	NM_135893.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tRNA-histidine guanylyltransferase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 31, 2011 this sequence version replaced NM_135893.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTGCA GCAGATTCGA GTACGTTAAA TCCTTTGAGC AGGACGACAG CATACTGCCT 
AATGTGTGGA TAGTCATACG AATAGACGGC AAAAAGTTCC ACAAGTTCTC CAAGACGCAC 
GATTTTGAAA AGCCCAACGA TGAAAATGCT CTCAATGTGA TGAATGCTGC TGCCACGGCG 
GTGATGCAGG AGTTTCGGGA TATTGTCCTC GCCTACGGTC AAAGTGATGA GTACTCCTTT 
GTTTTTCGCA AAGAGACGGC AGCGTTCAAG CGACGATCCG CCAAACTGCT CACCTACGTC 
ACCAGCTTGT TCTCCTCGAG CTATGTAATG CAATGGTCCA AGTGGATGAA TCTGCCATTG 
GCCTATGCTC CGTGTTTCGA TGGTCGCGTT GTTCTGTATC CTTCGGAGCA GAATCTAAAG 
GACTATCTCA GCTGGCGACA GGCCGATGTG CATGTGAACA ACCTGTACAA TACCGCCTTT 
TGGAAACTCG TTTTGGAAAA AGGCCTGACG AACCAGCAAG CCGAGGCGAA ACTCCGTGGC 
ACCTTTTCGG CGGACAAAAA TGAGCTGCTC TTCCAGGAAT TCGGGATAAA TTACAACAAT 
CTGCCGGCAA TGTACAGAAA GGGAACGATT CTGCTGAGAA AACGCGTGAT ATTGGGTGAG 
AAGAGCAGGC AGGCCGTGGT GCCGCTGCAC GAGGATCTGA TATCTTCGCA GTTCTGGAAG 
GAACACACCG AAATATTGGG AAAATATGTG CCAGGCACAT ACGACGCACC ACAAACTTTT 
CCCCGATTGG TGGAGATGCA AATAAATGGC AAGGATGACA ATGAGGCGGA GGAGCCACAA 
AATCTGGCTG GTACCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609737.1
CDS	96..956
Translation	MACSRFEYVKSFEQDDSILPNVWIVIRIDGKKFHKFSKTHDFEKPNDENALNVMNAAATAVMQEFRDIVLAYGQSDEYSFVFRKETAAFKRRSAKLLTYVTSLFSSSYVMQWSKWMNLPLAYAPCFDGRVVLYPSEQNLKDYLSWRQADVHVNNLYNTAFWKLVLEKGLTNQQAEAKLRGTFSADKNELLFQEFGINYNNLPAMYRKGTILLRKRVILGEKSRQAVVPLHEDLISSQFWKEHTEILGKYVPGTYDAPQTFPRLVEMQINGKDDNEAEEPQNLAGTS

Target ORF information:

RefSeq Version	NM_135893.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tRNA-histidine guanylyltransferase (THG), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135893.2

ORF Insert Sequence:

ATGGCCTGCA GCAGATTCGA GTACGTTAAA TCCTTTGAGC AGGACGACAG CATACTGCCT 
AATGTGTGGA TAGTCATACG AATAGACGGC AAAAAGTTCC ACAAGTTCTC CAAGACGCAC 
GATTTTGAAA AGCCCAACGA TGAAAATGCT CTCAATGTGA TGAATGCTGC TGCCACGGCG 
GTGATGCAGG AGTTTCGGGA TATTGTCCTC GCCTACGGTC AAAGTGATGA GTACTCCTTT 
GTTTTTCGCA AAGAGACGGC AGCGTTCAAG CGACGATCCG CCAAACTGCT CACCTACGTC 
ACCAGCTTGT TCTCCTCGAG CTATGTAATG CAATGGTCCA AGTGGATGAA TCTGCCATTG 
GCCTATGCTC CGTGTTTCGA TGGTCGCGTT GTTCTGTATC CTTCGGAGCA GAATCTAAAG 
GACTATCTCA GCTGGCGACA GGCCGATGTG CATGTGAACA ACCTGTACAA TACCGCCTTT 
TGGAAACTCG TTTTGGAAAA AGGCCTGACG AACCAGCAAG CCGAGGCGAA ACTCCGTGGC 
ACCTTTTCGG CGGACAAAAA TGAGCTGCTC TTCCAGGAAT TCGGGATAAA TTACAACAAT 
CTGCCGGCAA TGTACAGAAA GGGAACGATT CTGCTGAGAA AACGCGTGAT ATTGGGTGAG 
AAGAGCAGGC AGGCCGTGGT GCCGCTGCAC GAGGATCTGA TATCTTCGCA GTTCTGGAAG 
GAACACACCG AAATATTGGG AAAATATGTG CCAGGCACAT ACGACGCACC ACAAACTTTT 
CCCCGATTGG TGGAGATGCA AATAAATGGC AAGGATGACA ATGAGGCGGA GGAGCCACAA 
AATCTGGCTG GTACCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THG ( NM_001201886.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188815.1 Drosophila melanogaster tRNA-histidine guanylyltransferase (THG), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14700
Clone ID Related Accession (Same CDS sequence)	NM_135893.2 , NM_001201886.1
Accession Version	NM_001201886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tRNA-histidine guanylyltransferase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTGCA GCAGATTCGA GTACGTTAAA TCCTTTGAGC AGGACGACAG CATACTGCCT 
AATGTGTGGA TAGTCATACG AATAGACGGC AAAAAGTTCC ACAAGTTCTC CAAGACGCAC 
GATTTTGAAA AGCCCAACGA TGAAAATGCT CTCAATGTGA TGAATGCTGC TGCCACGGCG 
GTGATGCAGG AGTTTCGGGA TATTGTCCTC GCCTACGGTC AAAGTGATGA GTACTCCTTT 
GTTTTTCGCA AAGAGACGGC AGCGTTCAAG CGACGATCCG CCAAACTGCT CACCTACGTC 
ACCAGCTTGT TCTCCTCGAG CTATGTAATG CAATGGTCCA AGTGGATGAA TCTGCCATTG 
GCCTATGCTC CGTGTTTCGA TGGTCGCGTT GTTCTGTATC CTTCGGAGCA GAATCTAAAG 
GACTATCTCA GCTGGCGACA GGCCGATGTG CATGTGAACA ACCTGTACAA TACCGCCTTT 
TGGAAACTCG TTTTGGAAAA AGGCCTGACG AACCAGCAAG CCGAGGCGAA ACTCCGTGGC 
ACCTTTTCGG CGGACAAAAA TGAGCTGCTC TTCCAGGAAT TCGGGATAAA TTACAACAAT 
CTGCCGGCAA TGTACAGAAA GGGAACGATT CTGCTGAGAA AACGCGTGAT ATTGGGTGAG 
AAGAGCAGGC AGGCCGTGGT GCCGCTGCAC GAGGATCTGA TATCTTCGCA GTTCTGGAAG 
GAACACACCG AAATATTGGG AAAATATGTG CCAGGCACAT ACGACGCACC ACAAACTTTT 
CCCCGATTGG TGGAGATGCA AATAAATGGC AAGGATGACA ATGAGGCGGA GGAGCCACAA 
AATCTGGCTG GTACCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188815.1
CDS	23..883
Translation	MACSRFEYVKSFEQDDSILPNVWIVIRIDGKKFHKFSKTHDFEKPNDENALNVMNAAATAVMQEFRDIVLAYGQSDEYSFVFRKETAAFKRRSAKLLTYVTSLFSSSYVMQWSKWMNLPLAYAPCFDGRVVLYPSEQNLKDYLSWRQADVHVNNLYNTAFWKLVLEKGLTNQQAEAKLRGTFSADKNELLFQEFGINYNNLPAMYRKGTILLRKRVILGEKSRQAVVPLHEDLISSQFWKEHTEILGKYVPGTYDAPQTFPRLVEMQINGKDDNEAEEPQNLAGTS

Target ORF information:

RefSeq Version	NM_001201886.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tRNA-histidine guanylyltransferase (THG), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201886.1

ORF Insert Sequence:

ATGGCCTGCA GCAGATTCGA GTACGTTAAA TCCTTTGAGC AGGACGACAG CATACTGCCT 
AATGTGTGGA TAGTCATACG AATAGACGGC AAAAAGTTCC ACAAGTTCTC CAAGACGCAC 
GATTTTGAAA AGCCCAACGA TGAAAATGCT CTCAATGTGA TGAATGCTGC TGCCACGGCG 
GTGATGCAGG AGTTTCGGGA TATTGTCCTC GCCTACGGTC AAAGTGATGA GTACTCCTTT 
GTTTTTCGCA AAGAGACGGC AGCGTTCAAG CGACGATCCG CCAAACTGCT CACCTACGTC 
ACCAGCTTGT TCTCCTCGAG CTATGTAATG CAATGGTCCA AGTGGATGAA TCTGCCATTG 
GCCTATGCTC CGTGTTTCGA TGGTCGCGTT GTTCTGTATC CTTCGGAGCA GAATCTAAAG 
GACTATCTCA GCTGGCGACA GGCCGATGTG CATGTGAACA ACCTGTACAA TACCGCCTTT 
TGGAAACTCG TTTTGGAAAA AGGCCTGACG AACCAGCAAG CCGAGGCGAA ACTCCGTGGC 
ACCTTTTCGG CGGACAAAAA TGAGCTGCTC TTCCAGGAAT TCGGGATAAA TTACAACAAT 
CTGCCGGCAA TGTACAGAAA GGGAACGATT CTGCTGAGAA AACGCGTGAT ATTGGGTGAG 
AAGAGCAGGC AGGCCGTGGT GCCGCTGCAC GAGGATCTGA TATCTTCGCA GTTCTGGAAG 
GAACACACCG AAATATTGGG AAAATATGTG CCAGGCACAT ACGACGCACC ACAAACTTTT 
CCCCGATTGG TGGAGATGCA AATAAATGGC AAGGATGACA ATGAGGCGGA GGAGCCACAA 
AATCTGGCTG GTACCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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THG cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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