sash1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sash1a
Entrez Gene ID	557132
Full Name	SAM and SH3 domain containing 1a
Synonyms	fi33a01,ik:tdsubc_2e1,si:dkey-264g21.1,tdsubc_2e1,wu:fi33a01,xx:tdsubc_2e1
General protein information	Preferred Names SAM and SH3 domain containing 1a Names SAM and SH3 domain-containing protein 1 SAM and SH3 domain containing 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021468431.1	XP_021324106.1	SAM and SH3 domain-containing protein 1 isoform X1
XM_021468432.1	XP_021324107.1	SAM and SH3 domain-containing protein 1 isoform X2
XM_021468433.1	XP_021324108.1	SAM and SH3 domain-containing protein 1 isoform X3
XM_021468434.1	XP_021324109.1	SAM and SH3 domain-containing protein 1 isoform X4
XM_021468436.1	XP_021324111.1	SAM and SH3 domain-containing protein 1 isoform X6
XM_021468435.1	XP_021324110.1	SAM and SH3 domain-containing protein 1 isoform X5
NM_001044819.1	NP_001038284.1	SAM and SH3 domain-containing protein 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SASH1	XP_850394.1
Bos taurus (cattle)	SASH1	XP_002690361.1
Danio rerio (zebrafish)	sash1a	NP_001038284.1
Homo sapiens (human)	SASH1	NP_056093.3
	SASH1	XP_002803910.1
Gallus gallus (chicken)	SASH1	XP_004935653.1
Pan troglodytes (chimpanzee)	SASH1	XP_518791.3
Mus musculus (house mouse)	Sash1	NP_780364.3
Rattus norvegicus (Norway rat)	Sash1	XP_003748693.1
Xenopus tropicalis (tropical clawed frog)	sash1	XP_002936309.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Rab32 and Rab38 genes in chordate pigmentation: an evolutionary perspective.
Coppola U, Annona G, D'Aniello S, Ristoratore F
BMC evolutionary biology1626(2016 Jan)

Fmrp Interacts with Adar and Regulates RNA Editing, Synaptic Density and Locomotor Activity in Zebrafish.
Shamay-Ramot A, Khermesh K, Porath HT, Barak M, Pinto Y, Wachtel C, Zilberberg A, Lerer-Goldshtein T, Efroni S, Levanon EY, Appelbaum L
PLoS genetics11(12)e1005702(2015 Dec)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sash1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sash1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa150622	XM_021468432.1 Latest version!	Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa150623	XM_021468433.1 Latest version!	Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa150624	XM_021468434.1 Latest version!	Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa150625	XM_021468435.1 Latest version!	Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa150626	XM_021468436.1 Latest version!	Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa34904	NM_001044819.1 Latest version!	Danio rerio SAM and SH3 domain containing 1a (sash1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa150621	XM_021468431.1 Latest version!	Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sash1a ( XM_021468432.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324107.1 Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa150622
Clone ID Related Accession (Same CDS sequence)	XM_021468432.1
Accession Version	XM_021468432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SAM and SH3 domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACTA ATGGACCCAA CATCGTCTAC GAATGGCTAA AAACCCTTCA ACTTTGCCAA 
TATGTCGAGG CGTTTGTGGA TAACGGATAC GACGACTTGG AAGTTTGCAA ACAAATCGGA 
GACCCTGACC TGGATGCCAT CGGGGTTTTC ATTCCGCACC ACCGCCAGCG GATTCATGAT 
GCGGTGCAAA GGTTAAAAGA TGACGATAGA GAGACGGCTA CCGGGCTTTA TTTCACCCTA 
GAGCCGATGC CCCCGGTCTC ACCTGTATAC ACGAGTCGCA TGGCAGAGCA ATATGAGTCA 
AGGCTGCGGG GCTCCAGGTC ATGGACAGAG CAGAGCAGTG ATAGAGCCGC ACGCGGCGTG 
GGGTACATGG GGGCGTCCAG GAATCTGACC CTGGGGAACC GCAAGGAGCT GGAAATCTAC 
CCCAAACTGA AGCTGAAGAT CATGATCAGG GATAAGCTCA TTAGGGACGG GATAAACCTT 
GCCAGGCAGC CATACTCTAA TAAGGATGGC TCTCTGGGTA ATATAGATGA CCTGGCACAA 
GAGTACTCGG AGTACTACAA CACATACTTC AGTGACGTCA GCGATCGGAT GGAGGAACTG 
CGCAAACGGC AAGTCTCTCA AGAGCTAGAC ATGGAAAAAT CAGACACTAG TCTAAACTCT 
TCACAGCTCC GCTCAGACAT CCAGGATTCA TTTGGATTCA GCAGTGAGGT GTCCACACCA 
GAGGCTGAGA GAAAAATTTC TCTGCACAAA TCCAGCTCTG AGGATGGATC AGGAAAATGG 
GACAACAAGA AGAAAAACAA ATCCTTCTGG CAGAACTTCC GAAAGTCACA GAAGGGGGTC 
ACAAGGCAAG CGTCAAAAGG AGAAGACATT GGCTATGTGG CCAGTGAGAT CACAATGAGT 
GATGAAGAGC GAATCCAGCT TATGATGATG GTGAAAGAGA AGATGATCAC TGTAGAGGAA 
GCTCTGTCAC GGTCTGTGGA GCAGTCAGAT GATGAGCAGG AGGACTCTGT GAAATTCAAA 
AGGCTTCACA AGCTGGTGAA CTCCACTCGA CGTGTCCGCA AGAAACTCAT CAAAGTGGAT 
GATAGCAAGA GACATGGCTC ACAAGATTCA CTCAGTCTGG ATGCAGCTCC CATATGCGAC 
GATATAAATG CACTATATGC AGAAATCCAC AAGAAGCCGG CAAAGTGTAC AGACTCCTCT 
TTGTCCTCAC TTGCCCAAGA GCAGCTTTCT CTGGACGGGG ACACGGACAG CCTGAACACC 
TCGCCCTCCA CCAGCAGCCT TGATGCCTGG AAACCTGCTC ACAAATTAGT CAAGGCTCCC 
AGCACACATC CCCATGGCCT GATCCGGCCA GCACGCAAGA GCAGTGCTGG GTCTGGGCTG 
GGTGTAGCGG GGGGCAGTGG CTCCTCGTTT TCTGAATTGG ACGGTTTGGG AGACGACAAT 
GCTAAAGTCT CCCGCTCGGT TACAGACGGA GAGATGCGGA AGGCCTTGTC ATCTCTATCT 
CATGGGGTAA GTACTGATAG CGTTTACGCT TTTTATGGCC TCACTAGGCC CCGCCCAAAG 
CCACACCCCC TCCTGGTGTC TTTAGATGAC ATCAACAACA CCAAGCGGCC ACCTGTTTCC 
ATGTGGCAGC GAAATAAGCC TGACCCGAAC TATGCATACT CTACCAAACA CCTGCTTTAC 
CAACGTTCCT GCAACGCTCC GAAAACCGCA CCCTCCTCCA CCCTACCTGC GTCTCCTCCC 
ACTCGCGAGC TTCCGCTAGC CAAGGAGAAG GGGAAGAGTT GTGGGAGCGG GGTAGTGGGC 
AGCTGGCTGT TTCCACCAAG GCGTCTCAAA GGAAGGACGG CAGTTAGTGA GCTCAACATC 
ACATATGTGG TGGAACGCAG CCTGTACGGA CATTTGAACT GGACTGGGCT GGTCAGGCCA 
ATCACATTGA GCAAAGCGGA CAGACGTTGG CTCCTGGAGG ATGAAAGAGA TGCCAACAGG 
AAATGGGCAT CTAGCATGGA CCGCTGCACC AAGCGTGTGC TCTTGCGCAT CCAACAAAAG 
TCTAGAACAT GTAGCTTTGG AGGATTTGAC CTATCTAATC GTTCCCTCCA TGTGGTCAAC 
ACAGCAGAGC CTAGCGAGCA GGAGGCTCTG TACAGGGATG GCGTGAAATC TCCCACCACA 
TCCCGTATCT CTCTAGGAAA GAAAGTCAAG TCTGTCAAAG AGACTATGAG GAAACGCATG 
TCCAAGAAAT ATGTCGGGTC TCTCTCTGAA CAGTCCAGTC CAGATGGGAC TCCCAGTTGT 
CCTCAGTCTC CTCAACCAGA CACAGATTCT CTAGAGAAGC CCAAGCTCAA GGCAGGAGGG 
TCTGTGGAGA GTCTGCGCAG CTCTCTCAGT GGCCAAAGCT CCATGAGTGG GCAGACGGTG 
AGCACCACAG ACTCATCAGC CAGTAACAGA GAGAGTGTGA GGAGTGAGGA TGGTGATGAT 
GAAGAGCCTC CATACAGAGG CCCGTTCTGT GGCAGAGCAA GAGTTCACAC TGACTTCACA 
CCCAGTCCAT ATGATACAGA TTCTCTCAAG CTCAAGAGAG GAGATGTGAT TGACATCATC 
AGTAAGCCGC CCATGGGCAC ATGGATGGGT CTGCTCAATA ACAAAGTGGG CACGTTTAAG 
TTCATCTATG TGGATGTTCT GGCAGAAGAA GAGGAGAAGC CCAAACGAGC TGTTCGCAGG 
AGGAAGAAGG GCCGACCACC CAAACCCAGC TCAGTCGAGG AGCTTCTGGA GAGAATCAAC 
CTGAAGGAGC ACATGCCAAC GTTTCTGTTT AACGGGTATG AAGATCTGGA CACGTTTAAG 
TTACTAGAGG AAGAAGATCT GGATGAGCTC AACATCAAAG ACCCACAACA CCGAGCCGTA 
CTGATGACAG CTGTTGAACT GCTTCAGGAT TATGACAGCA GCAGTGATCC AGAGCGCAGC 
GGTCTCTCTG GCTCTCAGGA GAAGCTCCTC TGTGAGGGCC ATGGCCTCAT GGCTGACTCT 
CCACGGGACT CTGGTTGCTA CGAAAGCAAT GAAAACCTAG AGAATGGTAA GAGCCGAAAG 
GCATCGCGCC ACAGCCGTGG TTCATCAGGG TTTGACTGTG GGGATGTAAA GTCTCCAGAT 
TATCCCACTC TTCCCATGAC TCGCTCCACT GAAGCTCTTC AACAGCAGGC CAAGAAGGAG 
CGCAAGTTCT CGAAGACCTT TCTGCCCCGA CCCATGAAGG GCTTCAATCT GCGAAACCTC 
GGCCTCAAGA AAGGCTCCGC AGCAGCTCAA ACCTCCCGGA TTTCGCTCAG CAGAAGCTGT 
GAGGAGTTGG ACAGCCCCCA GGAGACCCCT GACTCTTGGA AGCGCTCTCA TTCTCTGGGG 
GATATCCACT GGGAACAGGC CTTCGATCAG AGGAAGGACC AGCAGGGCAG TGAGGATAGA 
GGCAGGCTGG AGAGCAGCAC AAAAGAAATG AAAGCAGATC TTGTAGACTC CAAACAGGAT 
GAGAAGACCA ATATGGCTAA AAGGCAGACT GGCTCCTTGC AGAGGCCTGC AGTTCCCACA 
CAGCTTCCTG TGAGTCCGGT CAGTTCATTG CCTGCTTCTC AGAGCTTATT CCGTCAACCT 
GTCTCTGCTC CGACAAGCCC AGCTCCAAGG ACTCACCCTA AAAAGCCACC CATCCCACCT 
CCAGTGCCTG CTAAAAAGTC CAAAGAACGT TTGCCGAATG GTCTGCGACA CCCTCCGCTG 
GTGCTGTCCG GTTCAGTGCC AAACACTCCA ACCCTACCCC CAAAGCCTAT ATATTCTCCG 
ACCACCCCCT CTCCCTCTGA GGGTACCCCA TCAACCCCAG CATCACCAGG TGAATCTGCA 
GCACCTCTGA GCCCACCGTG GCTGTCTGAC CTGCCTGAAT CAGCATGTCC TCAAATCCAT 
GGAGCTAAAA TCGCTCTCAG CAGGAAGATC TCCCATGCCA AGATGCTTGA CCTAGAGACT 
CTGCTGGAGG ACAGGATGCA CTCGGAAGGG ATCGACCTCA AAGAGGAGCC TTACTCTGAT 
AAACATGGCC GGTGTGGTAT TCCTCAGCTC TTAGTTCAGA GGTACTCAGA GGACTTCCAG 
CAGCCAGCGA AGGACGTGGC GTCCAGTCTT GATCAAATCA GAGTTAAACA ACTCCGCAAG 
GAGCACCGCA TGGCTATTCC CTCAGGAGGT TTGACTGACA TGTGTCGGAA GCCAGTGTCT 
CTGGTTCATG TGAGCTCTGT CTCTGATTGG CTCGTGTCCA TCGGAATGCC TATGTATTCT 
GCACCTCTAC TGGCTGCTGG CTTTGACACG TTGAGTCGTG TGTCGTCTTT AAATGAGGCG 
CAAGCGAGGG ACGCGGGCCT GAAGGAGGAA CATCACATAC GGATTCTTCT CAGTGAAGCT 
CAACTCGTCT CAGCCGCCAG CTCAGGACAG TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324107.1
CDS	916..5331
Translation	MMTNGPNIVYEWLKTLQLCQYVEAFVDNGYDDLEVCKQIGDPDLDAIGVFIPHHRQRIHDAVQRLKDDDRETATGLYFTLEPMPPVSPVYTSRMAEQYESRLRGSRSWTEQSSDRAARGVGYMGASRNLTLGNRKELEIYPKLKLKIMIRDKLIRDGINLARQPYSNKDGSLGNIDDLAQEYSEYYNTYFSDVSDRMEELRKRQVSQELDMEKSDTSLNSSQLRSDIQDSFGFSSEVSTPEAERKISLHKSSSEDGSGKWDNKKKNKSFWQNFRKSQKGVTRQASKGEDIGYVASEITMSDEERIQLMMMVKEKMITVEEALSRSVEQSDDEQEDSVKFKRLHKLVNSTRRVRKKLIKVDDSKRHGSQDSLSLDAAPICDDINALYAEIHKKPAKCTDSSLSSLAQEQLSLDGDTDSLNTSPSTSSLDAWKPAHKLVKAPSTHPHGLIRPARKSSAGSGLGVAGGSGSSFSELDGLGDDNAKVSRSVTDGEMRKALSSLSHGVSTDSVYAFYGLTRPRPKPHPLLVSLDDINNTKRPPVSMWQRNKPDPNYAYSTKHLLYQRSCNAPKTAPSSTLPASPPTRELPLAKEKGKSCGSGVVGSWLFPPRRLKGRTAVSELNITYVVERSLYGHLNWTGLVRPITLSKADRRWLLEDERDANRKWASSMDRCTKRVLLRIQQKSRTCSFGGFDLSNRSLHVVNTAEPSEQEALYRDGVKSPTTSRISLGKKVKSVKETMRKRMSKKYVGSLSEQSSPDGTPSCPQSPQPDTDSLEKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSASNRESVRSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKRGDVIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLAEEEEKPKRAVRRRKKGRPPKPSSVEELLERINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIKDPQHRAVLMTAVELLQDYDSSSDPERSGLSGSQEKLLCEGHGLMADSPRDSGCYESNENLENGKSRKASRHSRGSSGFDCGDVKSPDYPTLPMTRSTEALQQQAKKERKFSKTFLPRPMKGFNLRNLGLKKGSAAAQTSRISLSRSCEELDSPQETPDSWKRSHSLGDIHWEQAFDQRKDQQGSEDRGRLESSTKEMKADLVDSKQDEKTNMAKRQTGSLQRPAVPTQLPVSPVSSLPASQSLFRQPVSAPTSPAPRTHPKKPPIPPPVPAKKSKERLPNGLRHPPLVLSGSVPNTPTLPPKPIYSPTTPSPSEGTPSTPASPGESAAPLSPPWLSDLPESACPQIHGAKIALSRKISHAKMLDLETLLEDRMHSEGIDLKEEPYSDKHGRCGIPQLLVQRYSEDFQQPAKDVASSLDQIRVKQLRKEHRMAIPSGGLTDMCRKPVSLVHVSSVSDWLVSIGMPMYSAPLLAAGFDTLSRVSSLNEAQARDAGLKEEHHIRILLSEAQLVSAASSGQS

Target ORF information:

RefSeq Version	XM_021468432.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468432.1

ORF Insert Sequence:

ATGATGACTA ATGGACCCAA CATCGTCTAC GAATGGCTAA AAACCCTTCA ACTTTGCCAA 
TATGTCGAGG CGTTTGTGGA TAACGGATAC GACGACTTGG AAGTTTGCAA ACAAATCGGA 
GACCCTGACC TGGATGCCAT CGGGGTTTTC ATTCCGCACC ACCGCCAGCG GATTCATGAT 
GCGGTGCAAA GGTTAAAAGA TGACGATAGA GAGACGGCTA CCGGGCTTTA TTTCACCCTA 
GAGCCGATGC CCCCGGTCTC ACCTGTATAC ACGAGTCGCA TGGCAGAGCA ATATGAGTCA 
AGGCTGCGGG GCTCCAGGTC ATGGACAGAG CAGAGCAGTG ATAGAGCCGC ACGCGGCGTG 
GGGTACATGG GGGCGTCCAG GAATCTGACC CTGGGGAACC GCAAGGAGCT GGAAATCTAC 
CCCAAACTGA AGCTGAAGAT CATGATCAGG GATAAGCTCA TTAGGGACGG GATAAACCTT 
GCCAGGCAGC CATACTCTAA TAAGGATGGC TCTCTGGGTA ATATAGATGA CCTGGCACAA 
GAGTACTCGG AGTACTACAA CACATACTTC AGTGACGTCA GCGATCGGAT GGAGGAACTG 
CGCAAACGGC AAGTCTCTCA AGAGCTAGAC ATGGAAAAAT CAGACACTAG TCTAAACTCT 
TCACAGCTCC GCTCAGACAT CCAGGATTCA TTTGGATTCA GCAGTGAGGT GTCCACACCA 
GAGGCTGAGA GAAAAATTTC TCTGCACAAA TCCAGCTCTG AGGATGGATC AGGAAAATGG 
GACAACAAGA AGAAAAACAA ATCCTTCTGG CAGAACTTCC GAAAGTCACA GAAGGGGGTC 
ACAAGGCAAG CGTCAAAAGG AGAAGACATT GGCTATGTGG CCAGTGAGAT CACAATGAGT 
GATGAAGAGC GAATCCAGCT TATGATGATG GTGAAAGAGA AGATGATCAC TGTAGAGGAA 
GCTCTGTCAC GGTCTGTGGA GCAGTCAGAT GATGAGCAGG AGGACTCTGT GAAATTCAAA 
AGGCTTCACA AGCTGGTGAA CTCCACTCGA CGTGTCCGCA AGAAACTCAT CAAAGTGGAT 
GATAGCAAGA GACATGGCTC ACAAGATTCA CTCAGTCTGG ATGCAGCTCC CATATGCGAC 
GATATAAATG CACTATATGC AGAAATCCAC AAGAAGCCGG CAAAGTGTAC AGACTCCTCT 
TTGTCCTCAC TTGCCCAAGA GCAGCTTTCT CTGGACGGGG ACACGGACAG CCTGAACACC 
TCGCCCTCCA CCAGCAGCCT TGATGCCTGG AAACCTGCTC ACAAATTAGT CAAGGCTCCC 
AGCACACATC CCCATGGCCT GATCCGGCCA GCACGCAAGA GCAGTGCTGG GTCTGGGCTG 
GGTGTAGCGG GGGGCAGTGG CTCCTCGTTT TCTGAATTGG ACGGTTTGGG AGACGACAAT 
GCTAAAGTCT CCCGCTCGGT TACAGACGGA GAGATGCGGA AGGCCTTGTC ATCTCTATCT 
CATGGGGTAA GTACTGATAG CGTTTACGCT TTTTATGGCC TCACTAGGCC CCGCCCAAAG 
CCACACCCCC TCCTGGTGTC TTTAGATGAC ATCAACAACA CCAAGCGGCC ACCTGTTTCC 
ATGTGGCAGC GAAATAAGCC TGACCCGAAC TATGCATACT CTACCAAACA CCTGCTTTAC 
CAACGTTCCT GCAACGCTCC GAAAACCGCA CCCTCCTCCA CCCTACCTGC GTCTCCTCCC 
ACTCGCGAGC TTCCGCTAGC CAAGGAGAAG GGGAAGAGTT GTGGGAGCGG GGTAGTGGGC 
AGCTGGCTGT TTCCACCAAG GCGTCTCAAA GGAAGGACGG CAGTTAGTGA GCTCAACATC 
ACATATGTGG TGGAACGCAG CCTGTACGGA CATTTGAACT GGACTGGGCT GGTCAGGCCA 
ATCACATTGA GCAAAGCGGA CAGACGTTGG CTCCTGGAGG ATGAAAGAGA TGCCAACAGG 
AAATGGGCAT CTAGCATGGA CCGCTGCACC AAGCGTGTGC TCTTGCGCAT CCAACAAAAG 
TCTAGAACAT GTAGCTTTGG AGGATTTGAC CTATCTAATC GTTCCCTCCA TGTGGTCAAC 
ACAGCAGAGC CTAGCGAGCA GGAGGCTCTG TACAGGGATG GCGTGAAATC TCCCACCACA 
TCCCGTATCT CTCTAGGAAA GAAAGTCAAG TCTGTCAAAG AGACTATGAG GAAACGCATG 
TCCAAGAAAT ATGTCGGGTC TCTCTCTGAA CAGTCCAGTC CAGATGGGAC TCCCAGTTGT 
CCTCAGTCTC CTCAACCAGA CACAGATTCT CTAGAGAAGC CCAAGCTCAA GGCAGGAGGG 
TCTGTGGAGA GTCTGCGCAG CTCTCTCAGT GGCCAAAGCT CCATGAGTGG GCAGACGGTG 
AGCACCACAG ACTCATCAGC CAGTAACAGA GAGAGTGTGA GGAGTGAGGA TGGTGATGAT 
GAAGAGCCTC CATACAGAGG CCCGTTCTGT GGCAGAGCAA GAGTTCACAC TGACTTCACA 
CCCAGTCCAT ATGATACAGA TTCTCTCAAG CTCAAGAGAG GAGATGTGAT TGACATCATC 
AGTAAGCCGC CCATGGGCAC ATGGATGGGT CTGCTCAATA ACAAAGTGGG CACGTTTAAG 
TTCATCTATG TGGATGTTCT GGCAGAAGAA GAGGAGAAGC CCAAACGAGC TGTTCGCAGG 
AGGAAGAAGG GCCGACCACC CAAACCCAGC TCAGTCGAGG AGCTTCTGGA GAGAATCAAC 
CTGAAGGAGC ACATGCCAAC GTTTCTGTTT AACGGGTATG AAGATCTGGA CACGTTTAAG 
TTACTAGAGG AAGAAGATCT GGATGAGCTC AACATCAAAG ACCCACAACA CCGAGCCGTA 
CTGATGACAG CTGTTGAACT GCTTCAGGAT TATGACAGCA GCAGTGATCC AGAGCGCAGC 
GGTCTCTCTG GCTCTCAGGA GAAGCTCCTC TGTGAGGGCC ATGGCCTCAT GGCTGACTCT 
CCACGGGACT CTGGTTGCTA CGAAAGCAAT GAAAACCTAG AGAATGGTAA GAGCCGAAAG 
GCATCGCGCC ACAGCCGTGG TTCATCAGGG TTTGACTGTG GGGATGTAAA GTCTCCAGAT 
TATCCCACTC TTCCCATGAC TCGCTCCACT GAAGCTCTTC AACAGCAGGC CAAGAAGGAG 
CGCAAGTTCT CGAAGACCTT TCTGCCCCGA CCCATGAAGG GCTTCAATCT GCGAAACCTC 
GGCCTCAAGA AAGGCTCCGC AGCAGCTCAA ACCTCCCGGA TTTCGCTCAG CAGAAGCTGT 
GAGGAGTTGG ACAGCCCCCA GGAGACCCCT GACTCTTGGA AGCGCTCTCA TTCTCTGGGG 
GATATCCACT GGGAACAGGC CTTCGATCAG AGGAAGGACC AGCAGGGCAG TGAGGATAGA 
GGCAGGCTGG AGAGCAGCAC AAAAGAAATG AAAGCAGATC TTGTAGACTC CAAACAGGAT 
GAGAAGACCA ATATGGCTAA AAGGCAGACT GGCTCCTTGC AGAGGCCTGC AGTTCCCACA 
CAGCTTCCTG TGAGTCCGGT CAGTTCATTG CCTGCTTCTC AGAGCTTATT CCGTCAACCT 
GTCTCTGCTC CGACAAGCCC AGCTCCAAGG ACTCACCCTA AAAAGCCACC CATCCCACCT 
CCAGTGCCTG CTAAAAAGTC CAAAGAACGT TTGCCGAATG GTCTGCGACA CCCTCCGCTG 
GTGCTGTCCG GTTCAGTGCC AAACACTCCA ACCCTACCCC CAAAGCCTAT ATATTCTCCG 
ACCACCCCCT CTCCCTCTGA GGGTACCCCA TCAACCCCAG CATCACCAGG TGAATCTGCA 
GCACCTCTGA GCCCACCGTG GCTGTCTGAC CTGCCTGAAT CAGCATGTCC TCAAATCCAT 
GGAGCTAAAA TCGCTCTCAG CAGGAAGATC TCCCATGCCA AGATGCTTGA CCTAGAGACT 
CTGCTGGAGG ACAGGATGCA CTCGGAAGGG ATCGACCTCA AAGAGGAGCC TTACTCTGAT 
AAACATGGCC GGTGTGGTAT TCCTCAGCTC TTAGTTCAGA GGTACTCAGA GGACTTCCAG 
CAGCCAGCGA AGGACGTGGC GTCCAGTCTT GATCAAATCA GAGTTAAACA ACTCCGCAAG 
GAGCACCGCA TGGCTATTCC CTCAGGAGGT TTGACTGACA TGTGTCGGAA GCCAGTGTCT 
CTGGTTCATG TGAGCTCTGT CTCTGATTGG CTCGTGTCCA TCGGAATGCC TATGTATTCT 
GCACCTCTAC TGGCTGCTGG CTTTGACACG TTGAGTCGTG TGTCGTCTTT AAATGAGGCG 
CAAGCGAGGG ACGCGGGCCT GAAGGAGGAA CATCACATAC GGATTCTTCT CAGTGAAGCT 
CAACTCGTCT CAGCCGCCAG CTCAGGACAG TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sash1a ( XM_021468433.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324108.1 Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa150623
Clone ID Related Accession (Same CDS sequence)	XM_021468433.1
Accession Version	XM_021468433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SAM and SH3 domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGG ATCAAGTGTC TTCGGCCGAG GAGCCGCAGG CGGTCAGCGC CGCATCTTCG 
GACAGTTTCT CCCGGCTCTG GACGGACGTG ATGGGGATGC TGGATGGCTC TCTGGGTAAT 
ATAGATGACC TGGCACAAGA GTACTCGGAG TACTACAACA CATACTTCAG TGACGTCAGC 
GATCGGATGG AGGAACTGCG CAAACGGCAA GTCTCTCAAG AGCTAGACAT GGAAAAATCA 
GACACTAGTC TAAACTCTTC ACAGCTCCGC TCAGACATCC AGGATTCATT TGGATTCAGC 
AGTGAGGTGT CCACACCAGA GGCTGAGAGA AAAATTTCTC TGCACAAATC CAGCTCTGAG 
GATGGATCAG GAAAATGGGA CAACAAGAAG AAAAACAAAT CCTTCTGGCA GAACTTCCGA 
AAGTCACAGA AGGGGGTCAC AAGGCAAGCG TCAAAAGGAG AAGACATTGG CTATGTGGCC 
AGTGAGATCA CAATGAGTGA TGAAGAGCGA ATCCAGCTTA TGATGATGGT GAAAGAGAAG 
ATGATCACTG TAGAGGAAGC TCTGTCACGG TTAAAGGAAT ATGAGAGGAA CAGGCAGTTG 
AGCAGCAGTA CAGACACAGC AGAGTGGACT GATGGATCCA GTCCCACTGT TAACCTCTCC 
TCCACCTGCA ATTCTGTGGA GCAGTCAGAT GATGAGCAGG AGGACTCTGT GAAATTCAAA 
AGGCTTCACA AGCTGGTGAA CTCCACTCGA CGTGTCCGCA AGAAACTCAT CAAAGTGGAT 
GATAGCAAGA GACATGGCTC ACAAGATTCA CTCAGTCTGG ATGCAGCTCC CATATGCGAC 
GATATAAATG CACTATATGC AGAAATCCAC AAGAAGCCGG CAAAGTGTAC AGACTCCTCT 
TTGTCCTCAC TTGCCCAAGA GCAGCTTTCT CTGGACGGGG ACACGGACAG CCTGAACACC 
TCGCCCTCCA CCAGCAGCCT TGATGCCTGG AAACCTGCTC ACAAATTAGT CAAGGCTCCC 
AGCACACATC CCCATGGCCT GATCCGGCCA GCACGCAAGA GCAGTGCTGG GTCTGGGCTG 
GGTGTAGCGG GGGGCAGTGG CTCCTCGTTT TCTGAATTGG ACGGTTTGGG AGACGACAAT 
GCTAAAGTCT CCCGCTCGGT TACAGACGGA GAGATGCGGA AGGCCTTGTC ATCTCTATCT 
CATGGGGTAA GTACTGATAG CGTTTACGCT TTTTATGGCC TCACTAGGCC CCGCCCAAAG 
CCACACCCCC TCCTGGTGTC TTTAGATGAC ATCAACAACA CCAAGCGGCC ACCTGTTTCC 
ATGTGGCAGC GAAATAAGCC TGACCCGAAC TATGCATACT CTACCAAACA CCTGCTTTAC 
CAACGTTCCT GCAACGCTCC GAAAACCGCA CCCTCCTCCA CCCTACCTGC GTCTCCTCCC 
ACTCGCGAGC TTCCGCTAGC CAAGGAGAAG GGGAAGAGTT GTGGGAGCGG GGTAGTGGGC 
AGCTGGCTGT TTCCACCAAG GCGTCTCAAA GGAAGGACGG CAGTTAGTGA GCTCAACATC 
ACATATGTGG TGGAACGCAG CCTGTACGGA CATTTGAACT GGACTGGGCT GGTCAGGCCA 
ATCACATTGA GCAAAGCGGA CAGACGTTGG CTCCTGGAGG ATGAAAGAGA TGCCAACAGG 
AAATGGGCAT CTAGCATGGA CCGCTGCACC AAGCGTGTGC TCTTGCGCAT CCAACAAAAG 
TCTAGAACAT GTAGCTTTGG AGGATTTGAC CTATCTAATC GTTCCCTCCA TGTGGTCAAC 
ACAGCAGAGC CTAGCGAGCA GGAGGCTCTG TACAGGGATG GCGTGAAATC TCCCACCACA 
TCCCGTATCT CTCTAGGAAA GAAAGTCAAG TCTGTCAAAG AGACTATGAG GAAACGCATG 
TCCAAGAAAT ATGTCGGGTC TCTCTCTGAA CAGTCCAGTC CAGATGGGAC TCCCAGTTGT 
CCTCAGTCTC CTCAACCAGA CACAGATTCT CTAGAGAAGC CCAAGCTCAA GGCAGGAGGG 
TCTGTGGAGA GTCTGCGCAG CTCTCTCAGT GGCCAAAGCT CCATGAGTGG GCAGACGGTG 
AGCACCACAG ACTCATCAGC CAGTAACAGA GAGAGTGTGA GGAGTGAGGA TGGTGATGAT 
GAAGAGCCTC CATACAGAGG CCCGTTCTGT GGCAGAGCAA GAGTTCACAC TGACTTCACA 
CCCAGTCCAT ATGATACAGA TTCTCTCAAG CTCAAGAGAG GAGATGTGAT TGACATCATC 
AGTAAGCCGC CCATGGGCAC ATGGATGGGT CTGCTCAATA ACAAAGTGGG CACGTTTAAG 
TTCATCTATG TGGATGTTCT GGCAGAAGAA GAGGAGAAGC CCAAACGAGC TGTTCGCAGG 
AGGAAGAAGG GCCGACCACC CAAACCCAGC TCAGTCGAGG AGCTTCTGGA GAGAATCAAC 
CTGAAGGAGC ACATGCCAAC GTTTCTGTTT AACGGGTATG AAGATCTGGA CACGTTTAAG 
TTACTAGAGG AAGAAGATCT GGATGAGCTC AACATCAAAG ACCCACAACA CCGAGCCGTA 
CTGATGACAG CTGTTGAACT GCTTCAGGAT TATGACAGCA GCAGTGATCC AGAGCGCAGC 
GGTCTCTCTG GCTCTCAGGA GAAGCTCCTC TGTGAGGGCC ATGGCCTCAT GGCTGACTCT 
CCACGGGACT CTGGTTGCTA CGAAAGCAAT GAAAACCTAG AGAATGGTAA GAGCCGAAAG 
GCATCGCGCC ACAGCCGTGG TTCATCAGGG TTTGACTGTG GGGATGTAAA GTCTCCAGAT 
TATCCCACTC TTCCCATGAC TCGCTCCACT GAAGCTCTTC AACAGCAGGC CAAGAAGGAG 
CGCAAGTTCT CGAAGACCTT TCTGCCCCGA CCCATGAAGG GCTTCAATCT GCGAAACCTC 
GGCCTCAAGA AAGGCTCCGC AGCAGCTCAA ACCTCCCGGA TTTCGCTCAG CAGAAGCTGT 
GAGGAGTTGG ACAGCCCCCA GGAGACCCCT GACTCTTGGA AGCGCTCTCA TTCTCTGGGG 
GATATCCACT GGGAACAGGC CTTCGATCAG AGGAAGGACC AGCAGGGCAG TGAGGATAGA 
GGCAGGCTGG AGAGCAGCAC AAAAGAAATG AAAGCAGATC TTGTAGACTC CAAACAGGAT 
GAGAAGACCA ATATGGCTAA AAGGCAGACT GGCTCCTTGC AGAGGCCTGC AGTTCCCACA 
CAGCTTCCTG TGAGTCCGGT CAGTTCATTG CCTGCTTCTC AGAGCTTATT CCGTCAACCT 
GTCTCTGCTC CGACAAGCCC AGCTCCAAGG ACTCACCCTA AAAAGCCACC CATCCCACCT 
CCAGTGCCTG CTAAAAAGTC CAAAGAACGT TTGCCGAATG GTCTGCGACA CCCTCCGCTG 
GTGCTGTCCG GTTCAGTGCC AAACACTCCA ACCCTACCCC CAAAGCCTAT ATATTCTCCG 
ACCACCCCCT CTCCCTCTGA GGGTACCCCA TCAACCCCAG CATCACCAGG TGAATCTGCA 
GCACCTCTGA GCCCACCGTG GCTGTCTGAC CTGCCTGAAT CAGCATGTCC TCAAATCCAT 
GGAGCTAAAA TCGCTCTCAG CAGGAAGATC TCCCATGCCA AGATGCTTGA CCTAGAGACT 
CTGCTGGAGG ACAGGATGCA CTCGGAAGGG ATCGACCTCA AAGAGGAGCC TTACTCTGAT 
AAACATGGCC GGTGTGGTAT TCCTCAGCTC TTAGTTCAGA GGTACTCAGA GGACTTCCAG 
CAGCCAGCGA AGGACGTGGC GTCCAGTCTT GATCAAATCA GAGTTAAACA ACTCCGCAAG 
GAGCACCGCA TGGCTATTCC CTCAGGAGGT TTGACTGACA TGTGTCGGAA GCCAGTGTCT 
CTGGTTCATG TGAGCTCTGT CTCTGATTGG CTCGTGTCCA TCGGAATGCC TATGTATTCT 
GCACCTCTAC TGGCTGCTGG CTTTGACACG TTGAGTCGTG TGTCGTCTTT AAATGAGGCG 
CAAGCGAGGG ACGCGGGCCT GAAGGAGGAA CATCACATAC GGATTCTTCT CAGTGAAGCT 
CAACTCGTCT CAGCCGCCAG CTCAGGACAG TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324108.1
CDS	617..4732
Translation	MEEDQVSSAEEPQAVSAASSDSFSRLWTDVMGMLDGSLGNIDDLAQEYSEYYNTYFSDVSDRMEELRKRQVSQELDMEKSDTSLNSSQLRSDIQDSFGFSSEVSTPEAERKISLHKSSSEDGSGKWDNKKKNKSFWQNFRKSQKGVTRQASKGEDIGYVASEITMSDEERIQLMMMVKEKMITVEEALSRLKEYERNRQLSSSTDTAEWTDGSSPTVNLSSTCNSVEQSDDEQEDSVKFKRLHKLVNSTRRVRKKLIKVDDSKRHGSQDSLSLDAAPICDDINALYAEIHKKPAKCTDSSLSSLAQEQLSLDGDTDSLNTSPSTSSLDAWKPAHKLVKAPSTHPHGLIRPARKSSAGSGLGVAGGSGSSFSELDGLGDDNAKVSRSVTDGEMRKALSSLSHGVSTDSVYAFYGLTRPRPKPHPLLVSLDDINNTKRPPVSMWQRNKPDPNYAYSTKHLLYQRSCNAPKTAPSSTLPASPPTRELPLAKEKGKSCGSGVVGSWLFPPRRLKGRTAVSELNITYVVERSLYGHLNWTGLVRPITLSKADRRWLLEDERDANRKWASSMDRCTKRVLLRIQQKSRTCSFGGFDLSNRSLHVVNTAEPSEQEALYRDGVKSPTTSRISLGKKVKSVKETMRKRMSKKYVGSLSEQSSPDGTPSCPQSPQPDTDSLEKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSASNRESVRSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKRGDVIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLAEEEEKPKRAVRRRKKGRPPKPSSVEELLERINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIKDPQHRAVLMTAVELLQDYDSSSDPERSGLSGSQEKLLCEGHGLMADSPRDSGCYESNENLENGKSRKASRHSRGSSGFDCGDVKSPDYPTLPMTRSTEALQQQAKKERKFSKTFLPRPMKGFNLRNLGLKKGSAAAQTSRISLSRSCEELDSPQETPDSWKRSHSLGDIHWEQAFDQRKDQQGSEDRGRLESSTKEMKADLVDSKQDEKTNMAKRQTGSLQRPAVPTQLPVSPVSSLPASQSLFRQPVSAPTSPAPRTHPKKPPIPPPVPAKKSKERLPNGLRHPPLVLSGSVPNTPTLPPKPIYSPTTPSPSEGTPSTPASPGESAAPLSPPWLSDLPESACPQIHGAKIALSRKISHAKMLDLETLLEDRMHSEGIDLKEEPYSDKHGRCGIPQLLVQRYSEDFQQPAKDVASSLDQIRVKQLRKEHRMAIPSGGLTDMCRKPVSLVHVSSVSDWLVSIGMPMYSAPLLAAGFDTLSRVSSLNEAQARDAGLKEEHHIRILLSEAQLVSAASSGQS

Target ORF information:

RefSeq Version	XM_021468433.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468433.1

ORF Insert Sequence:

ATGGAAGAGG ATCAAGTGTC TTCGGCCGAG GAGCCGCAGG CGGTCAGCGC CGCATCTTCG 
GACAGTTTCT CCCGGCTCTG GACGGACGTG ATGGGGATGC TGGATGGCTC TCTGGGTAAT 
ATAGATGACC TGGCACAAGA GTACTCGGAG TACTACAACA CATACTTCAG TGACGTCAGC 
GATCGGATGG AGGAACTGCG CAAACGGCAA GTCTCTCAAG AGCTAGACAT GGAAAAATCA 
GACACTAGTC TAAACTCTTC ACAGCTCCGC TCAGACATCC AGGATTCATT TGGATTCAGC 
AGTGAGGTGT CCACACCAGA GGCTGAGAGA AAAATTTCTC TGCACAAATC CAGCTCTGAG 
GATGGATCAG GAAAATGGGA CAACAAGAAG AAAAACAAAT CCTTCTGGCA GAACTTCCGA 
AAGTCACAGA AGGGGGTCAC AAGGCAAGCG TCAAAAGGAG AAGACATTGG CTATGTGGCC 
AGTGAGATCA CAATGAGTGA TGAAGAGCGA ATCCAGCTTA TGATGATGGT GAAAGAGAAG 
ATGATCACTG TAGAGGAAGC TCTGTCACGG TTAAAGGAAT ATGAGAGGAA CAGGCAGTTG 
AGCAGCAGTA CAGACACAGC AGAGTGGACT GATGGATCCA GTCCCACTGT TAACCTCTCC 
TCCACCTGCA ATTCTGTGGA GCAGTCAGAT GATGAGCAGG AGGACTCTGT GAAATTCAAA 
AGGCTTCACA AGCTGGTGAA CTCCACTCGA CGTGTCCGCA AGAAACTCAT CAAAGTGGAT 
GATAGCAAGA GACATGGCTC ACAAGATTCA CTCAGTCTGG ATGCAGCTCC CATATGCGAC 
GATATAAATG CACTATATGC AGAAATCCAC AAGAAGCCGG CAAAGTGTAC AGACTCCTCT 
TTGTCCTCAC TTGCCCAAGA GCAGCTTTCT CTGGACGGGG ACACGGACAG CCTGAACACC 
TCGCCCTCCA CCAGCAGCCT TGATGCCTGG AAACCTGCTC ACAAATTAGT CAAGGCTCCC 
AGCACACATC CCCATGGCCT GATCCGGCCA GCACGCAAGA GCAGTGCTGG GTCTGGGCTG 
GGTGTAGCGG GGGGCAGTGG CTCCTCGTTT TCTGAATTGG ACGGTTTGGG AGACGACAAT 
GCTAAAGTCT CCCGCTCGGT TACAGACGGA GAGATGCGGA AGGCCTTGTC ATCTCTATCT 
CATGGGGTAA GTACTGATAG CGTTTACGCT TTTTATGGCC TCACTAGGCC CCGCCCAAAG 
CCACACCCCC TCCTGGTGTC TTTAGATGAC ATCAACAACA CCAAGCGGCC ACCTGTTTCC 
ATGTGGCAGC GAAATAAGCC TGACCCGAAC TATGCATACT CTACCAAACA CCTGCTTTAC 
CAACGTTCCT GCAACGCTCC GAAAACCGCA CCCTCCTCCA CCCTACCTGC GTCTCCTCCC 
ACTCGCGAGC TTCCGCTAGC CAAGGAGAAG GGGAAGAGTT GTGGGAGCGG GGTAGTGGGC 
AGCTGGCTGT TTCCACCAAG GCGTCTCAAA GGAAGGACGG CAGTTAGTGA GCTCAACATC 
ACATATGTGG TGGAACGCAG CCTGTACGGA CATTTGAACT GGACTGGGCT GGTCAGGCCA 
ATCACATTGA GCAAAGCGGA CAGACGTTGG CTCCTGGAGG ATGAAAGAGA TGCCAACAGG 
AAATGGGCAT CTAGCATGGA CCGCTGCACC AAGCGTGTGC TCTTGCGCAT CCAACAAAAG 
TCTAGAACAT GTAGCTTTGG AGGATTTGAC CTATCTAATC GTTCCCTCCA TGTGGTCAAC 
ACAGCAGAGC CTAGCGAGCA GGAGGCTCTG TACAGGGATG GCGTGAAATC TCCCACCACA 
TCCCGTATCT CTCTAGGAAA GAAAGTCAAG TCTGTCAAAG AGACTATGAG GAAACGCATG 
TCCAAGAAAT ATGTCGGGTC TCTCTCTGAA CAGTCCAGTC CAGATGGGAC TCCCAGTTGT 
CCTCAGTCTC CTCAACCAGA CACAGATTCT CTAGAGAAGC CCAAGCTCAA GGCAGGAGGG 
TCTGTGGAGA GTCTGCGCAG CTCTCTCAGT GGCCAAAGCT CCATGAGTGG GCAGACGGTG 
AGCACCACAG ACTCATCAGC CAGTAACAGA GAGAGTGTGA GGAGTGAGGA TGGTGATGAT 
GAAGAGCCTC CATACAGAGG CCCGTTCTGT GGCAGAGCAA GAGTTCACAC TGACTTCACA 
CCCAGTCCAT ATGATACAGA TTCTCTCAAG CTCAAGAGAG GAGATGTGAT TGACATCATC 
AGTAAGCCGC CCATGGGCAC ATGGATGGGT CTGCTCAATA ACAAAGTGGG CACGTTTAAG 
TTCATCTATG TGGATGTTCT GGCAGAAGAA GAGGAGAAGC CCAAACGAGC TGTTCGCAGG 
AGGAAGAAGG GCCGACCACC CAAACCCAGC TCAGTCGAGG AGCTTCTGGA GAGAATCAAC 
CTGAAGGAGC ACATGCCAAC GTTTCTGTTT AACGGGTATG AAGATCTGGA CACGTTTAAG 
TTACTAGAGG AAGAAGATCT GGATGAGCTC AACATCAAAG ACCCACAACA CCGAGCCGTA 
CTGATGACAG CTGTTGAACT GCTTCAGGAT TATGACAGCA GCAGTGATCC AGAGCGCAGC 
GGTCTCTCTG GCTCTCAGGA GAAGCTCCTC TGTGAGGGCC ATGGCCTCAT GGCTGACTCT 
CCACGGGACT CTGGTTGCTA CGAAAGCAAT GAAAACCTAG AGAATGGTAA GAGCCGAAAG 
GCATCGCGCC ACAGCCGTGG TTCATCAGGG TTTGACTGTG GGGATGTAAA GTCTCCAGAT 
TATCCCACTC TTCCCATGAC TCGCTCCACT GAAGCTCTTC AACAGCAGGC CAAGAAGGAG 
CGCAAGTTCT CGAAGACCTT TCTGCCCCGA CCCATGAAGG GCTTCAATCT GCGAAACCTC 
GGCCTCAAGA AAGGCTCCGC AGCAGCTCAA ACCTCCCGGA TTTCGCTCAG CAGAAGCTGT 
GAGGAGTTGG ACAGCCCCCA GGAGACCCCT GACTCTTGGA AGCGCTCTCA TTCTCTGGGG 
GATATCCACT GGGAACAGGC CTTCGATCAG AGGAAGGACC AGCAGGGCAG TGAGGATAGA 
GGCAGGCTGG AGAGCAGCAC AAAAGAAATG AAAGCAGATC TTGTAGACTC CAAACAGGAT 
GAGAAGACCA ATATGGCTAA AAGGCAGACT GGCTCCTTGC AGAGGCCTGC AGTTCCCACA 
CAGCTTCCTG TGAGTCCGGT CAGTTCATTG CCTGCTTCTC AGAGCTTATT CCGTCAACCT 
GTCTCTGCTC CGACAAGCCC AGCTCCAAGG ACTCACCCTA AAAAGCCACC CATCCCACCT 
CCAGTGCCTG CTAAAAAGTC CAAAGAACGT TTGCCGAATG GTCTGCGACA CCCTCCGCTG 
GTGCTGTCCG GTTCAGTGCC AAACACTCCA ACCCTACCCC CAAAGCCTAT ATATTCTCCG 
ACCACCCCCT CTCCCTCTGA GGGTACCCCA TCAACCCCAG CATCACCAGG TGAATCTGCA 
GCACCTCTGA GCCCACCGTG GCTGTCTGAC CTGCCTGAAT CAGCATGTCC TCAAATCCAT 
GGAGCTAAAA TCGCTCTCAG CAGGAAGATC TCCCATGCCA AGATGCTTGA CCTAGAGACT 
CTGCTGGAGG ACAGGATGCA CTCGGAAGGG ATCGACCTCA AAGAGGAGCC TTACTCTGAT 
AAACATGGCC GGTGTGGTAT TCCTCAGCTC TTAGTTCAGA GGTACTCAGA GGACTTCCAG 
CAGCCAGCGA AGGACGTGGC GTCCAGTCTT GATCAAATCA GAGTTAAACA ACTCCGCAAG 
GAGCACCGCA TGGCTATTCC CTCAGGAGGT TTGACTGACA TGTGTCGGAA GCCAGTGTCT 
CTGGTTCATG TGAGCTCTGT CTCTGATTGG CTCGTGTCCA TCGGAATGCC TATGTATTCT 
GCACCTCTAC TGGCTGCTGG CTTTGACACG TTGAGTCGTG TGTCGTCTTT AAATGAGGCG 
CAAGCGAGGG ACGCGGGCCT GAAGGAGGAA CATCACATAC GGATTCTTCT CAGTGAAGCT 
CAACTCGTCT CAGCCGCCAG CTCAGGACAG TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sash1a ( XM_021468434.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324109.1 Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X4, mRNA.

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CloneID	ODa150624
Clone ID Related Accession (Same CDS sequence)	XM_021468434.1
Accession Version	XM_021468434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SAM and SH3 domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGCT CTCTGGGTAA TATAGATGAC CTGGCACAAG AGTACTCGGA GTACTACAAC 
ACATACTTCA GTGACGTCAG CGATCGGATG GAGGAACTGC GCAAACGGCA AGTCTCTCAA 
GAGCTAGACA TGGAAAAATC AGACACTAGT CTAAACTCTT CACAGCTCCG CTCAGACATC 
CAGGATTCAT TTGGATTCAG CAGTGAGGTG TCCACACCAG AGGCTGAGAG AAAAATTTCT 
CTGCACAAAT CCAGCTCTGA GGATGGATCA GGAAAATGGG ACAACAAGAA GAAAAACAAA 
TCCTTCTGGC AGAACTTCCG AAAGTCACAG AAGGGGGTCA CAAGGCAAGC GTCAAAAGGA 
GAAGACATTG GCTATGTGGC CAGTGAGATC ACAATGAGTG ATGAAGAGCG AATCCAGCTT 
ATGATGATGG TGAAAGAGAA GATGATCACT GTAGAGGAAG CTCTGTCACG GTTAAAGGAA 
TATGAGAGGA ACAGGCAGTT GAGCAGCAGT ACAGACACAG CAGAGTGGAC TGATGGATCC 
AGTCCCACTG TTAACCTCTC CTCCACCTGC AATTCTGTGG AGCAGTCAGA TGATGAGCAG 
GAGGACTCTG TGAAATTCAA AAGGCTTCAC AAGCTGGTGA ACTCCACTCG ACGTGTCCGC 
AAGAAACTCA TCAAAGTGGA TGATAGCAAG AGACATGGCT CACAAGATTC ACTCAGTCTG 
GATGCAGCTC CCATATGCGA CGATATAAAT GCACTATATG CAGAAATCCA CAAGAAGCCG 
GCAAAGTGTA CAGACTCCTC TTTGTCCTCA CTTGCCCAAG AGCAGCTTTC TCTGGACGGG 
GACACGGACA GCCTGAACAC CTCGCCCTCC ACCAGCAGCC TTGATGCCTG GAAACCTGCT 
CACAAATTAG TCAAGGCTCC CAGCACACAT CCCCATGGCC TGATCCGGCC AGCACGCAAG 
AGCAGTGCTG GGTCTGGGCT GGGTGTAGCG GGGGGCAGTG GCTCCTCGTT TTCTGAATTG 
GACGGTTTGG GAGACGACAA TGCTAAAGTC TCCCGCTCGG TTACAGACGG AGAGATGCGG 
AAGGCCTTGT CATCTCTATC TCATGGGGTA AGTACTGATA GCGTTTACGC TTTTTATGGC 
CTCACTAGGC CCCGCCCAAA GCCACACCCC CTCCTGGTGT CTTTAGATGA CATCAACAAC 
ACCAAGCGGC CACCTGTTTC CATGTGGCAG CGAAATAAGC CTGACCCGAA CTATGCATAC 
TCTACCAAAC ACCTGCTTTA CCAACGTTCC TGCAACGCTC CGAAAACCGC ACCCTCCTCC 
ACCCTACCTG CGTCTCCTCC CACTCGCGAG CTTCCGCTAG CCAAGGAGAA GGGGAAGAGT 
TGTGGGAGCG GGGTAGTGGG CAGCTGGCTG TTTCCACCAA GGCGTCTCAA AGGAAGGACG 
GCAGTTAGTG AGCTCAACAT CACATATGTG GTGGAACGCA GCCTGTACGG ACATTTGAAC 
TGGACTGGGC TGGTCAGGCC AATCACATTG AGCAAAGCGG ACAGACGTTG GCTCCTGGAG 
GATGAAAGAG ATGCCAACAG GAAATGGGCA TCTAGCATGG ACCGCTGCAC CAAGCGTGTG 
CTCTTGCGCA TCCAACAAAA GTCTAGAACA TGTAGCTTTG GAGGATTTGA CCTATCTAAT 
CGTTCCCTCC ATGTGGTCAA CACAGCAGAG CCTAGCGAGC AGGAGGCTCT GTACAGGGAT 
GGCGTGAAAT CTCCCACCAC ATCCCGTATC TCTCTAGGAA AGAAAGTCAA GTCTGTCAAA 
GAGACTATGA GGAAACGCAT GTCCAAGAAA TATGTCGGGT CTCTCTCTGA ACAGTCCAGT 
CCAGATGGGA CTCCCAGTTG TCCTCAGTCT CCTCAACCAG ACACAGATTC TCTAGAGAAG 
CCCAAGCTCA AGGCAGGAGG GTCTGTGGAG AGTCTGCGCA GCTCTCTCAG TGGCCAAAGC 
TCCATGAGTG GGCAGACGGT GAGCACCACA GACTCATCAG CCAGTAACAG AGAGAGTGTG 
AGGAGTGAGG ATGGTGATGA TGAAGAGCCT CCATACAGAG GCCCGTTCTG TGGCAGAGCA 
AGAGTTCACA CTGACTTCAC ACCCAGTCCA TATGATACAG ATTCTCTCAA GCTCAAGAGA 
GGAGATGTGA TTGACATCAT CAGTAAGCCG CCCATGGGCA CATGGATGGG TCTGCTCAAT 
AACAAAGTGG GCACGTTTAA GTTCATCTAT GTGGATGTTC TGGCAGAAGA AGAGGAGAAG 
CCCAAACGAG CTGTTCGCAG GAGGAAGAAG GGCCGACCAC CCAAACCCAG CTCAGTCGAG 
GAGCTTCTGG AGAGAATCAA CCTGAAGGAG CACATGCCAA CGTTTCTGTT TAACGGGTAT 
GAAGATCTGG ACACGTTTAA GTTACTAGAG GAAGAAGATC TGGATGAGCT CAACATCAAA 
GACCCACAAC ACCGAGCCGT ACTGATGACA GCTGTTGAAC TGCTTCAGGA TTATGACAGC 
AGCAGTGATC CAGAGCGCAG CGGTCTCTCT GGCTCTCAGG AGAAGCTCCT CTGTGAGGGC 
CATGGCCTCA TGGCTGACTC TCCACGGGAC TCTGGTTGCT ACGAAAGCAA TGAAAACCTA 
GAGAATGGTA AGAGCCGAAA GGCATCGCGC CACAGCCGTG GTTCATCAGG GTTTGACTGT 
GGGGATGTAA AGTCTCCAGA TTATCCCACT CTTCCCATGA CTCGCTCCAC TGAAGCTCTT 
CAACAGCAGG CCAAGAAGGA GCGCAAGTTC TCGAAGACCT TTCTGCCCCG ACCCATGAAG 
GGCTTCAATC TGCGAAACCT CGGCCTCAAG AAAGGCTCCG CAGCAGCTCA AACCTCCCGG 
ATTTCGCTCA GCAGAAGCTG TGAGGAGTTG GACAGCCCCC AGGAGACCCC TGACTCTTGG 
AAGCGCTCTC ATTCTCTGGG GGATATCCAC TGGGAACAGG CCTTCGATCA GAGGAAGGAC 
CAGCAGGGCA GTGAGGATAG AGGCAGGCTG GAGAGCAGCA CAAAAGAAAT GAAAGCAGAT 
CTTGTAGACT CCAAACAGGA TGAGAAGACC AATATGGCTA AAAGGCAGAC TGGCTCCTTG 
CAGAGGCCTG CAGTTCCCAC ACAGCTTCCT GTGAGTCCGG TCAGTTCATT GCCTGCTTCT 
CAGAGCTTAT TCCGTCAACC TGTCTCTGCT CCGACAAGCC CAGCTCCAAG GACTCACCCT 
AAAAAGCCAC CCATCCCACC TCCAGTGCCT GCTAAAAAGT CCAAAGAACG TTTGCCGAAT 
GGTCTGCGAC ACCCTCCGCT GGTGCTGTCC GGTTCAGTGC CAAACACTCC AACCCTACCC 
CCAAAGCCTA TATATTCTCC GACCACCCCC TCTCCCTCTG AGGGTACCCC ATCAACCCCA 
GCATCACCAG GTGAATCTGC AGCACCTCTG AGCCCACCGT GGCTGTCTGA CCTGCCTGAA 
TCAGCATGTC CTCAAATCCA TGGAGCTAAA ATCGCTCTCA GCAGGAAGAT CTCCCATGCC 
AAGATGCTTG ACCTAGAGAC TCTGCTGGAG GACAGGATGC ACTCGGAAGG GATCGACCTC 
AAAGAGGAGC CTTACTCTGA TAAACATGGC CGGTGTGGTA TTCCTCAGCT CTTAGTTCAG 
AGGTACTCAG AGGACTTCCA GCAGCCAGCG AAGGACGTGG CGTCCAGTCT TGATCAAATC 
AGAGTTAAAC AACTCCGCAA GGAGCACCGC ATGGCTATTC CCTCAGGAGG TTTGACTGAC 
ATGTGTCGGA AGCCAGTGTC TCTGGTTCAT GTGAGCTCTG TCTCTGATTG GCTCGTGTCC 
ATCGGAATGC CTATGTATTC TGCACCTCTA CTGGCTGCTG GCTTTGACAC GTTGAGTCGT 
GTGTCGTCTT TAAATGAGGC GCAAGCGAGG GACGCGGGCC TGAAGGAGGA ACATCACATA 
CGGATTCTTC TCAGTGAAGC TCAACTCGTC TCAGCCGCCA GCTCAGGACA GTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324109.1
CDS	167..4183
Translation	MDGSLGNIDDLAQEYSEYYNTYFSDVSDRMEELRKRQVSQELDMEKSDTSLNSSQLRSDIQDSFGFSSEVSTPEAERKISLHKSSSEDGSGKWDNKKKNKSFWQNFRKSQKGVTRQASKGEDIGYVASEITMSDEERIQLMMMVKEKMITVEEALSRLKEYERNRQLSSSTDTAEWTDGSSPTVNLSSTCNSVEQSDDEQEDSVKFKRLHKLVNSTRRVRKKLIKVDDSKRHGSQDSLSLDAAPICDDINALYAEIHKKPAKCTDSSLSSLAQEQLSLDGDTDSLNTSPSTSSLDAWKPAHKLVKAPSTHPHGLIRPARKSSAGSGLGVAGGSGSSFSELDGLGDDNAKVSRSVTDGEMRKALSSLSHGVSTDSVYAFYGLTRPRPKPHPLLVSLDDINNTKRPPVSMWQRNKPDPNYAYSTKHLLYQRSCNAPKTAPSSTLPASPPTRELPLAKEKGKSCGSGVVGSWLFPPRRLKGRTAVSELNITYVVERSLYGHLNWTGLVRPITLSKADRRWLLEDERDANRKWASSMDRCTKRVLLRIQQKSRTCSFGGFDLSNRSLHVVNTAEPSEQEALYRDGVKSPTTSRISLGKKVKSVKETMRKRMSKKYVGSLSEQSSPDGTPSCPQSPQPDTDSLEKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSASNRESVRSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKRGDVIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLAEEEEKPKRAVRRRKKGRPPKPSSVEELLERINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIKDPQHRAVLMTAVELLQDYDSSSDPERSGLSGSQEKLLCEGHGLMADSPRDSGCYESNENLENGKSRKASRHSRGSSGFDCGDVKSPDYPTLPMTRSTEALQQQAKKERKFSKTFLPRPMKGFNLRNLGLKKGSAAAQTSRISLSRSCEELDSPQETPDSWKRSHSLGDIHWEQAFDQRKDQQGSEDRGRLESSTKEMKADLVDSKQDEKTNMAKRQTGSLQRPAVPTQLPVSPVSSLPASQSLFRQPVSAPTSPAPRTHPKKPPIPPPVPAKKSKERLPNGLRHPPLVLSGSVPNTPTLPPKPIYSPTTPSPSEGTPSTPASPGESAAPLSPPWLSDLPESACPQIHGAKIALSRKISHAKMLDLETLLEDRMHSEGIDLKEEPYSDKHGRCGIPQLLVQRYSEDFQQPAKDVASSLDQIRVKQLRKEHRMAIPSGGLTDMCRKPVSLVHVSSVSDWLVSIGMPMYSAPLLAAGFDTLSRVSSLNEAQARDAGLKEEHHIRILLSEAQLVSAASSGQS

Target ORF information:

RefSeq Version	XM_021468434.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468434.1

ORF Insert Sequence:

ATGGATGGCT CTCTGGGTAA TATAGATGAC CTGGCACAAG AGTACTCGGA GTACTACAAC 
ACATACTTCA GTGACGTCAG CGATCGGATG GAGGAACTGC GCAAACGGCA AGTCTCTCAA 
GAGCTAGACA TGGAAAAATC AGACACTAGT CTAAACTCTT CACAGCTCCG CTCAGACATC 
CAGGATTCAT TTGGATTCAG CAGTGAGGTG TCCACACCAG AGGCTGAGAG AAAAATTTCT 
CTGCACAAAT CCAGCTCTGA GGATGGATCA GGAAAATGGG ACAACAAGAA GAAAAACAAA 
TCCTTCTGGC AGAACTTCCG AAAGTCACAG AAGGGGGTCA CAAGGCAAGC GTCAAAAGGA 
GAAGACATTG GCTATGTGGC CAGTGAGATC ACAATGAGTG ATGAAGAGCG AATCCAGCTT 
ATGATGATGG TGAAAGAGAA GATGATCACT GTAGAGGAAG CTCTGTCACG GTTAAAGGAA 
TATGAGAGGA ACAGGCAGTT GAGCAGCAGT ACAGACACAG CAGAGTGGAC TGATGGATCC 
AGTCCCACTG TTAACCTCTC CTCCACCTGC AATTCTGTGG AGCAGTCAGA TGATGAGCAG 
GAGGACTCTG TGAAATTCAA AAGGCTTCAC AAGCTGGTGA ACTCCACTCG ACGTGTCCGC 
AAGAAACTCA TCAAAGTGGA TGATAGCAAG AGACATGGCT CACAAGATTC ACTCAGTCTG 
GATGCAGCTC CCATATGCGA CGATATAAAT GCACTATATG CAGAAATCCA CAAGAAGCCG 
GCAAAGTGTA CAGACTCCTC TTTGTCCTCA CTTGCCCAAG AGCAGCTTTC TCTGGACGGG 
GACACGGACA GCCTGAACAC CTCGCCCTCC ACCAGCAGCC TTGATGCCTG GAAACCTGCT 
CACAAATTAG TCAAGGCTCC CAGCACACAT CCCCATGGCC TGATCCGGCC AGCACGCAAG 
AGCAGTGCTG GGTCTGGGCT GGGTGTAGCG GGGGGCAGTG GCTCCTCGTT TTCTGAATTG 
GACGGTTTGG GAGACGACAA TGCTAAAGTC TCCCGCTCGG TTACAGACGG AGAGATGCGG 
AAGGCCTTGT CATCTCTATC TCATGGGGTA AGTACTGATA GCGTTTACGC TTTTTATGGC 
CTCACTAGGC CCCGCCCAAA GCCACACCCC CTCCTGGTGT CTTTAGATGA CATCAACAAC 
ACCAAGCGGC CACCTGTTTC CATGTGGCAG CGAAATAAGC CTGACCCGAA CTATGCATAC 
TCTACCAAAC ACCTGCTTTA CCAACGTTCC TGCAACGCTC CGAAAACCGC ACCCTCCTCC 
ACCCTACCTG CGTCTCCTCC CACTCGCGAG CTTCCGCTAG CCAAGGAGAA GGGGAAGAGT 
TGTGGGAGCG GGGTAGTGGG CAGCTGGCTG TTTCCACCAA GGCGTCTCAA AGGAAGGACG 
GCAGTTAGTG AGCTCAACAT CACATATGTG GTGGAACGCA GCCTGTACGG ACATTTGAAC 
TGGACTGGGC TGGTCAGGCC AATCACATTG AGCAAAGCGG ACAGACGTTG GCTCCTGGAG 
GATGAAAGAG ATGCCAACAG GAAATGGGCA TCTAGCATGG ACCGCTGCAC CAAGCGTGTG 
CTCTTGCGCA TCCAACAAAA GTCTAGAACA TGTAGCTTTG GAGGATTTGA CCTATCTAAT 
CGTTCCCTCC ATGTGGTCAA CACAGCAGAG CCTAGCGAGC AGGAGGCTCT GTACAGGGAT 
GGCGTGAAAT CTCCCACCAC ATCCCGTATC TCTCTAGGAA AGAAAGTCAA GTCTGTCAAA 
GAGACTATGA GGAAACGCAT GTCCAAGAAA TATGTCGGGT CTCTCTCTGA ACAGTCCAGT 
CCAGATGGGA CTCCCAGTTG TCCTCAGTCT CCTCAACCAG ACACAGATTC TCTAGAGAAG 
CCCAAGCTCA AGGCAGGAGG GTCTGTGGAG AGTCTGCGCA GCTCTCTCAG TGGCCAAAGC 
TCCATGAGTG GGCAGACGGT GAGCACCACA GACTCATCAG CCAGTAACAG AGAGAGTGTG 
AGGAGTGAGG ATGGTGATGA TGAAGAGCCT CCATACAGAG GCCCGTTCTG TGGCAGAGCA 
AGAGTTCACA CTGACTTCAC ACCCAGTCCA TATGATACAG ATTCTCTCAA GCTCAAGAGA 
GGAGATGTGA TTGACATCAT CAGTAAGCCG CCCATGGGCA CATGGATGGG TCTGCTCAAT 
AACAAAGTGG GCACGTTTAA GTTCATCTAT GTGGATGTTC TGGCAGAAGA AGAGGAGAAG 
CCCAAACGAG CTGTTCGCAG GAGGAAGAAG GGCCGACCAC CCAAACCCAG CTCAGTCGAG 
GAGCTTCTGG AGAGAATCAA CCTGAAGGAG CACATGCCAA CGTTTCTGTT TAACGGGTAT 
GAAGATCTGG ACACGTTTAA GTTACTAGAG GAAGAAGATC TGGATGAGCT CAACATCAAA 
GACCCACAAC ACCGAGCCGT ACTGATGACA GCTGTTGAAC TGCTTCAGGA TTATGACAGC 
AGCAGTGATC CAGAGCGCAG CGGTCTCTCT GGCTCTCAGG AGAAGCTCCT CTGTGAGGGC 
CATGGCCTCA TGGCTGACTC TCCACGGGAC TCTGGTTGCT ACGAAAGCAA TGAAAACCTA 
GAGAATGGTA AGAGCCGAAA GGCATCGCGC CACAGCCGTG GTTCATCAGG GTTTGACTGT 
GGGGATGTAA AGTCTCCAGA TTATCCCACT CTTCCCATGA CTCGCTCCAC TGAAGCTCTT 
CAACAGCAGG CCAAGAAGGA GCGCAAGTTC TCGAAGACCT TTCTGCCCCG ACCCATGAAG 
GGCTTCAATC TGCGAAACCT CGGCCTCAAG AAAGGCTCCG CAGCAGCTCA AACCTCCCGG 
ATTTCGCTCA GCAGAAGCTG TGAGGAGTTG GACAGCCCCC AGGAGACCCC TGACTCTTGG 
AAGCGCTCTC ATTCTCTGGG GGATATCCAC TGGGAACAGG CCTTCGATCA GAGGAAGGAC 
CAGCAGGGCA GTGAGGATAG AGGCAGGCTG GAGAGCAGCA CAAAAGAAAT GAAAGCAGAT 
CTTGTAGACT CCAAACAGGA TGAGAAGACC AATATGGCTA AAAGGCAGAC TGGCTCCTTG 
CAGAGGCCTG CAGTTCCCAC ACAGCTTCCT GTGAGTCCGG TCAGTTCATT GCCTGCTTCT 
CAGAGCTTAT TCCGTCAACC TGTCTCTGCT CCGACAAGCC CAGCTCCAAG GACTCACCCT 
AAAAAGCCAC CCATCCCACC TCCAGTGCCT GCTAAAAAGT CCAAAGAACG TTTGCCGAAT 
GGTCTGCGAC ACCCTCCGCT GGTGCTGTCC GGTTCAGTGC CAAACACTCC AACCCTACCC 
CCAAAGCCTA TATATTCTCC GACCACCCCC TCTCCCTCTG AGGGTACCCC ATCAACCCCA 
GCATCACCAG GTGAATCTGC AGCACCTCTG AGCCCACCGT GGCTGTCTGA CCTGCCTGAA 
TCAGCATGTC CTCAAATCCA TGGAGCTAAA ATCGCTCTCA GCAGGAAGAT CTCCCATGCC 
AAGATGCTTG ACCTAGAGAC TCTGCTGGAG GACAGGATGC ACTCGGAAGG GATCGACCTC 
AAAGAGGAGC CTTACTCTGA TAAACATGGC CGGTGTGGTA TTCCTCAGCT CTTAGTTCAG 
AGGTACTCAG AGGACTTCCA GCAGCCAGCG AAGGACGTGG CGTCCAGTCT TGATCAAATC 
AGAGTTAAAC AACTCCGCAA GGAGCACCGC ATGGCTATTC CCTCAGGAGG TTTGACTGAC 
ATGTGTCGGA AGCCAGTGTC TCTGGTTCAT GTGAGCTCTG TCTCTGATTG GCTCGTGTCC 
ATCGGAATGC CTATGTATTC TGCACCTCTA CTGGCTGCTG GCTTTGACAC GTTGAGTCGT 
GTGTCGTCTT TAAATGAGGC GCAAGCGAGG GACGCGGGCC TGAAGGAGGA ACATCACATA 
CGGATTCTTC TCAGTGAAGC TCAACTCGTC TCAGCCGCCA GCTCAGGACA GTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sash1a ( XM_021468435.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324110.1 Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X5, mRNA.

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CloneID	ODa150625
Clone ID Related Accession (Same CDS sequence)	XM_021468435.1
Accession Version	XM_021468435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SAM and SH3 domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACTA ATGGACCCAA CATCGTCTAC GAATGGCTAA AAACCCTTCA ACTTTGCCAA 
TATGTCGAGG CGTTTGTGGA TAACGGATAC GACGACTTGG AAGTTTGCAA ACAAATCGGA 
GACCCTGACC TGGATGCCAT CGGGGTTTTC ATTCCGCACC ACCGCCAGCG GATTCATGAT 
GCGGTGCAAA GGTTAAAAGA TGACGATAGA GAGACGGCTA CCGGGCTTTA TTTCACCCTA 
GAGCCGATGC CCCCGGTCTC ACCTGTATAC ACGAGTCGCA TGGCAGAGCA ATATGAGTCA 
AGGCTGCGGG GCTCCAGGTC ATGGACAGAG CAGAGCAGTG ATAGAGCCGC ACGCGGCGTG 
GGGTACATGG GGGCGTCCAG GAATCTGACC CTGGGGAACC GCAAGGAGCT GGAAATCTAC 
CCCAAACTGA AGCTGAAGAT CATGATCAGG GATAAGCTCA TTAGGGACGG GATAAACCTT 
GCCAGGCAGC CATACTCTAA TAAGGATGGC TCTCTGGGTA ATATAGATGA CCTGGCACAA 
GAGTACTCGG AGTACTACAA CACATACTTC AGTGACGTCA GCGATCGGAT GGAGGAACTG 
CGCAAACGGC AAGTCTCTCA AGAGCTAGAC ATGGAAAAAT CAGACACTAG TCTAAACTCT 
TCACAGCTCC GCTCAGACAT CCAGGATTCA TTTGGATTCA GCAGTGAGGT GTCCACACCA 
GAGGCTGAGA GAAAAATTTC TCTGCACAAA TCCAGCTCTG AGGATGGATC AGGAAAATGG 
GACAACAAGA AGAAAAACAA ATCCTTCTGG CAGAACTTCC GAAAGTCACA GAAGGGGGTC 
ACAAGGCAAG CGTCAAAAGG AGAAGACATT GGCTATGTGG CCAGTGAGAT CACAATGAGT 
GATGAAGAGC GAATCCAGCT TATGATGATG GTGAAAGAGA AGATGATCAC TGTAGAGGAA 
GCTCTGTCAC GGTTAAAGGA ATATGAGAGG AACAGGCAGT TGAGCAGCAG TACAGACACA 
GCAGAGTGGA CTGATGGATC CAGTCCCACT GTTAACCTCT CCTCCACCTG CAATTCTGTG 
GAGCAGTCAG ATGATGAGCA GGAGGACTCT GTGAAATTCA AAAGGCTTCA CAAGCTGGTG 
AACTCCACTC GACGTGTCCG CAAGAAACTC ATCAAAGTGG ATGATAGCAA GAGACATGGC 
TCACAAGATT CACTCAGTCT GGATGCAGCT CCCATATGCG ACGATATAAA TGCACTATAT 
GCAGAAATCC ACAAGAAGCC GGCAAAGTGT ACAGACTCCT CTTTGTCCTC ACTTGCCCAA 
GAGCAGCTTT CTCTGGACGG GGACACGGAC AGCCTGAACA CCTCGCCCTC CACCAGCAGC 
CTTGATGCCT GGAAACCTGC TCACAAATTA GTCAAGGCTC CCAGCACACA TCCCCATGGC 
CTGATCCGGC CAGCACGCAA GAGCAGTGCT GGGTCTGGGC TGGGTGTAGC GGGGGGCAGT 
GGCTCCTCGT TTTCTGAATT GGACGGTTTG GGAGACGACA ATGCTAAAGT CTCCCGCTCG 
GTTACAGACG GAGAGATGCG GAAGGCCTTG TCATCTCTAT CTCATGGGAG AACATGTAGC 
TTTGGAGGAT TTGACCTATC TAATCGTTCC CTCCATGTGG TCAACACAGC AGAGCCTAGC 
GAGCAGGAGG CTCTGTACAG GGATGGCGTG AAATCTCCCA CCACATCCCG TATCTCTCTA 
GGAAAGAAAG TCAAGTCTGT CAAAGAGACT ATGAGGAAAC GCATGTCCAA GAAATATGTC 
GGGTCTCTCT CTGAACAGTC CAGTCCAGAT GGGACTCCCA GTTGTCCTCA GTCTCCTCAA 
CCAGACACAG ATTCTCTAGA GAAGCCCAAG CTCAAGGCAG GAGGGTCTGT GGAGAGTCTG 
CGCAGCTCTC TCAGTGGCCA AAGCTCCATG AGTGGGCAGA CGGTGAGCAC CACAGACTCA 
TCAGCCAGTA ACAGAGAGAG TGTGAGGAGT GAGGATGGTG ATGATGAAGA GCCTCCATAC 
AGAGGCCCGT TCTGTGGCAG AGCAAGAGTT CACACTGACT TCACACCCAG TCCATATGAT 
ACAGATTCTC TCAAGCTCAA GAGAGGAGAT GTGATTGACA TCATCAGTAA GCCGCCCATG 
GGCACATGGA TGGGTCTGCT CAATAACAAA GTGGGCACGT TTAAGTTCAT CTATGTGGAT 
GTTCTGGCAG AAGAAGAGGA GAAGCCCAAA CGAGCTGTTC GCAGGAGGAA GAAGGGCCGA 
CCACCCAAAC CCAGCTCAGT CGAGGAGCTT CTGGAGAGAA TCAACCTGAA GGAGCACATG 
CCAACGTTTC TGTTTAACGG GTATGAAGAT CTGGACACGT TTAAGTTACT AGAGGAAGAA 
GATCTGGATG AGCTCAACAT CAAAGACCCA CAACACCGAG CCGTACTGAT GACAGCTGTT 
GAACTGCTTC AGGATTATGA CAGCAGCAGT GATCCAGAGC GCAGCGGTCT CTCTGGCTCT 
CAGGAGAAGC TCCTCTGTGA GGGCCATGGC CTCATGGCTG ACTCTCCACG GGACTCTGGT 
TGCTACGAAA GCAATGAAAA CCTAGAGAAT GGTAAGAGCC GAAAGGCATC GCGCCACAGC 
CGTGGTTCAT CAGGGTTTGA CTGTGGGGAT GTAAAGTCTC CAGATTATCC CACTCTTCCC 
ATGACTCGCT CCACTGAAGC TCTTCAACAG CAGGCCAAGA AGGAGCGCAA GTTCTCGAAG 
ACCTTTCTGC CCCGACCCAT GAAGGGCTTC AATCTGCGAA ACCTCGGCCT CAAGAAAGGC 
TCCGCAGCAG CTCAAACCTC CCGGATTTCG CTCAGCAGAA GCTGTGAGGA GTTGGACAGC 
CCCCAGGAGA CCCCTGACTC TTGGAAGCGC TCTCATTCTC TGGGGGATAT CCACTGGGAA 
CAGGCCTTCG ATCAGAGGAA GGACCAGCAG GGCAGTGAGG ATAGAGGCAG GCTGGAGAGC 
AGCACAAAAG AAATGAAAGC AGATCTTGTA GACTCCAAAC AGGATGAGAA GACCAATATG 
GCTAAAAGGC AGACTGGCTC CTTGCAGAGG CCTGCAGTTC CCACACAGCT TCCTGTGAGT 
CCGGTCAGTT CATTGCCTGC TTCTCAGAGC TTATTCCGTC AACCTGTCTC TGCTCCGACA 
AGCCCAGCTC CAAGGACTCA CCCTAAAAAG CCACCCATCC CACCTCCAGT GCCTGCTAAA 
AAGTCCAAAG AACGTTTGCC GAATGGTCTG CGACACCCTC CGCTGGTGCT GTCCGGTTCA 
GTGCCAAACA CTCCAACCCT ACCCCCAAAG CCTATATATT CTCCGACCAC CCCCTCTCCC 
TCTGAGGGTA CCCCATCAAC CCCAGCATCA CCAGGTGAAT CTGCAGCACC TCTGAGCCCA 
CCGTGGCTGT CTGACCTGCC TGAATCAGCA TGTCCTCAAA TCCATGGAGC TAAAATCGCT 
CTCAGCAGGA AGATCTCCCA TGCCAAGATG CTTGACCTAG AGACTCTGCT GGAGGACAGG 
ATGCACTCGG AAGGGATCGA CCTCAAAGAG GAGCCTTACT CTGATAAACA TGGCCGGTGT 
GGTATTCCTC AGCTCTTAGT TCAGAGGTAC TCAGAGGACT TCCAGCAGCC AGCGAAGGAC 
GTGGCGTCCA GTCTTGATCA AATCAGAGTT AAACAACTCC GCAAGGAGCA CCGCATGGCT 
ATTCCCTCAG GAGGTTTGAC TGACATGTGT CGGAAGCCAG TGTCTCTGGT TCATGTGAGC 
TCTGTCTCTG ATTGGCTCGT GTCCATCGGA ATGCCTATGT ATTCTGCACC TCTACTGGCT 
GCTGGCTTTG ACACGTTGAG TCGTGTGTCG TCTTTAAATG AGGCGCAAGC GAGGGACGCG 
GGCCTGAAGG AGGAACATCA CATACGGATT CTTCTCAGTG AAGCTCAACT CGTCTCAGCC 
GCCAGCTCAG GACAGTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324110.1
CDS	914..4894
Translation	MMTNGPNIVYEWLKTLQLCQYVEAFVDNGYDDLEVCKQIGDPDLDAIGVFIPHHRQRIHDAVQRLKDDDRETATGLYFTLEPMPPVSPVYTSRMAEQYESRLRGSRSWTEQSSDRAARGVGYMGASRNLTLGNRKELEIYPKLKLKIMIRDKLIRDGINLARQPYSNKDGSLGNIDDLAQEYSEYYNTYFSDVSDRMEELRKRQVSQELDMEKSDTSLNSSQLRSDIQDSFGFSSEVSTPEAERKISLHKSSSEDGSGKWDNKKKNKSFWQNFRKSQKGVTRQASKGEDIGYVASEITMSDEERIQLMMMVKEKMITVEEALSRLKEYERNRQLSSSTDTAEWTDGSSPTVNLSSTCNSVEQSDDEQEDSVKFKRLHKLVNSTRRVRKKLIKVDDSKRHGSQDSLSLDAAPICDDINALYAEIHKKPAKCTDSSLSSLAQEQLSLDGDTDSLNTSPSTSSLDAWKPAHKLVKAPSTHPHGLIRPARKSSAGSGLGVAGGSGSSFSELDGLGDDNAKVSRSVTDGEMRKALSSLSHGRTCSFGGFDLSNRSLHVVNTAEPSEQEALYRDGVKSPTTSRISLGKKVKSVKETMRKRMSKKYVGSLSEQSSPDGTPSCPQSPQPDTDSLEKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSASNRESVRSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKRGDVIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLAEEEEKPKRAVRRRKKGRPPKPSSVEELLERINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIKDPQHRAVLMTAVELLQDYDSSSDPERSGLSGSQEKLLCEGHGLMADSPRDSGCYESNENLENGKSRKASRHSRGSSGFDCGDVKSPDYPTLPMTRSTEALQQQAKKERKFSKTFLPRPMKGFNLRNLGLKKGSAAAQTSRISLSRSCEELDSPQETPDSWKRSHSLGDIHWEQAFDQRKDQQGSEDRGRLESSTKEMKADLVDSKQDEKTNMAKRQTGSLQRPAVPTQLPVSPVSSLPASQSLFRQPVSAPTSPAPRTHPKKPPIPPPVPAKKSKERLPNGLRHPPLVLSGSVPNTPTLPPKPIYSPTTPSPSEGTPSTPASPGESAAPLSPPWLSDLPESACPQIHGAKIALSRKISHAKMLDLETLLEDRMHSEGIDLKEEPYSDKHGRCGIPQLLVQRYSEDFQQPAKDVASSLDQIRVKQLRKEHRMAIPSGGLTDMCRKPVSLVHVSSVSDWLVSIGMPMYSAPLLAAGFDTLSRVSSLNEAQARDAGLKEEHHIRILLSEAQLVSAASSGQS

Target ORF information:

RefSeq Version	XM_021468435.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468435.1

ORF Insert Sequence:

ATGATGACTA ATGGACCCAA CATCGTCTAC GAATGGCTAA AAACCCTTCA ACTTTGCCAA 
TATGTCGAGG CGTTTGTGGA TAACGGATAC GACGACTTGG AAGTTTGCAA ACAAATCGGA 
GACCCTGACC TGGATGCCAT CGGGGTTTTC ATTCCGCACC ACCGCCAGCG GATTCATGAT 
GCGGTGCAAA GGTTAAAAGA TGACGATAGA GAGACGGCTA CCGGGCTTTA TTTCACCCTA 
GAGCCGATGC CCCCGGTCTC ACCTGTATAC ACGAGTCGCA TGGCAGAGCA ATATGAGTCA 
AGGCTGCGGG GCTCCAGGTC ATGGACAGAG CAGAGCAGTG ATAGAGCCGC ACGCGGCGTG 
GGGTACATGG GGGCGTCCAG GAATCTGACC CTGGGGAACC GCAAGGAGCT GGAAATCTAC 
CCCAAACTGA AGCTGAAGAT CATGATCAGG GATAAGCTCA TTAGGGACGG GATAAACCTT 
GCCAGGCAGC CATACTCTAA TAAGGATGGC TCTCTGGGTA ATATAGATGA CCTGGCACAA 
GAGTACTCGG AGTACTACAA CACATACTTC AGTGACGTCA GCGATCGGAT GGAGGAACTG 
CGCAAACGGC AAGTCTCTCA AGAGCTAGAC ATGGAAAAAT CAGACACTAG TCTAAACTCT 
TCACAGCTCC GCTCAGACAT CCAGGATTCA TTTGGATTCA GCAGTGAGGT GTCCACACCA 
GAGGCTGAGA GAAAAATTTC TCTGCACAAA TCCAGCTCTG AGGATGGATC AGGAAAATGG 
GACAACAAGA AGAAAAACAA ATCCTTCTGG CAGAACTTCC GAAAGTCACA GAAGGGGGTC 
ACAAGGCAAG CGTCAAAAGG AGAAGACATT GGCTATGTGG CCAGTGAGAT CACAATGAGT 
GATGAAGAGC GAATCCAGCT TATGATGATG GTGAAAGAGA AGATGATCAC TGTAGAGGAA 
GCTCTGTCAC GGTTAAAGGA ATATGAGAGG AACAGGCAGT TGAGCAGCAG TACAGACACA 
GCAGAGTGGA CTGATGGATC CAGTCCCACT GTTAACCTCT CCTCCACCTG CAATTCTGTG 
GAGCAGTCAG ATGATGAGCA GGAGGACTCT GTGAAATTCA AAAGGCTTCA CAAGCTGGTG 
AACTCCACTC GACGTGTCCG CAAGAAACTC ATCAAAGTGG ATGATAGCAA GAGACATGGC 
TCACAAGATT CACTCAGTCT GGATGCAGCT CCCATATGCG ACGATATAAA TGCACTATAT 
GCAGAAATCC ACAAGAAGCC GGCAAAGTGT ACAGACTCCT CTTTGTCCTC ACTTGCCCAA 
GAGCAGCTTT CTCTGGACGG GGACACGGAC AGCCTGAACA CCTCGCCCTC CACCAGCAGC 
CTTGATGCCT GGAAACCTGC TCACAAATTA GTCAAGGCTC CCAGCACACA TCCCCATGGC 
CTGATCCGGC CAGCACGCAA GAGCAGTGCT GGGTCTGGGC TGGGTGTAGC GGGGGGCAGT 
GGCTCCTCGT TTTCTGAATT GGACGGTTTG GGAGACGACA ATGCTAAAGT CTCCCGCTCG 
GTTACAGACG GAGAGATGCG GAAGGCCTTG TCATCTCTAT CTCATGGGAG AACATGTAGC 
TTTGGAGGAT TTGACCTATC TAATCGTTCC CTCCATGTGG TCAACACAGC AGAGCCTAGC 
GAGCAGGAGG CTCTGTACAG GGATGGCGTG AAATCTCCCA CCACATCCCG TATCTCTCTA 
GGAAAGAAAG TCAAGTCTGT CAAAGAGACT ATGAGGAAAC GCATGTCCAA GAAATATGTC 
GGGTCTCTCT CTGAACAGTC CAGTCCAGAT GGGACTCCCA GTTGTCCTCA GTCTCCTCAA 
CCAGACACAG ATTCTCTAGA GAAGCCCAAG CTCAAGGCAG GAGGGTCTGT GGAGAGTCTG 
CGCAGCTCTC TCAGTGGCCA AAGCTCCATG AGTGGGCAGA CGGTGAGCAC CACAGACTCA 
TCAGCCAGTA ACAGAGAGAG TGTGAGGAGT GAGGATGGTG ATGATGAAGA GCCTCCATAC 
AGAGGCCCGT TCTGTGGCAG AGCAAGAGTT CACACTGACT TCACACCCAG TCCATATGAT 
ACAGATTCTC TCAAGCTCAA GAGAGGAGAT GTGATTGACA TCATCAGTAA GCCGCCCATG 
GGCACATGGA TGGGTCTGCT CAATAACAAA GTGGGCACGT TTAAGTTCAT CTATGTGGAT 
GTTCTGGCAG AAGAAGAGGA GAAGCCCAAA CGAGCTGTTC GCAGGAGGAA GAAGGGCCGA 
CCACCCAAAC CCAGCTCAGT CGAGGAGCTT CTGGAGAGAA TCAACCTGAA GGAGCACATG 
CCAACGTTTC TGTTTAACGG GTATGAAGAT CTGGACACGT TTAAGTTACT AGAGGAAGAA 
GATCTGGATG AGCTCAACAT CAAAGACCCA CAACACCGAG CCGTACTGAT GACAGCTGTT 
GAACTGCTTC AGGATTATGA CAGCAGCAGT GATCCAGAGC GCAGCGGTCT CTCTGGCTCT 
CAGGAGAAGC TCCTCTGTGA GGGCCATGGC CTCATGGCTG ACTCTCCACG GGACTCTGGT 
TGCTACGAAA GCAATGAAAA CCTAGAGAAT GGTAAGAGCC GAAAGGCATC GCGCCACAGC 
CGTGGTTCAT CAGGGTTTGA CTGTGGGGAT GTAAAGTCTC CAGATTATCC CACTCTTCCC 
ATGACTCGCT CCACTGAAGC TCTTCAACAG CAGGCCAAGA AGGAGCGCAA GTTCTCGAAG 
ACCTTTCTGC CCCGACCCAT GAAGGGCTTC AATCTGCGAA ACCTCGGCCT CAAGAAAGGC 
TCCGCAGCAG CTCAAACCTC CCGGATTTCG CTCAGCAGAA GCTGTGAGGA GTTGGACAGC 
CCCCAGGAGA CCCCTGACTC TTGGAAGCGC TCTCATTCTC TGGGGGATAT CCACTGGGAA 
CAGGCCTTCG ATCAGAGGAA GGACCAGCAG GGCAGTGAGG ATAGAGGCAG GCTGGAGAGC 
AGCACAAAAG AAATGAAAGC AGATCTTGTA GACTCCAAAC AGGATGAGAA GACCAATATG 
GCTAAAAGGC AGACTGGCTC CTTGCAGAGG CCTGCAGTTC CCACACAGCT TCCTGTGAGT 
CCGGTCAGTT CATTGCCTGC TTCTCAGAGC TTATTCCGTC AACCTGTCTC TGCTCCGACA 
AGCCCAGCTC CAAGGACTCA CCCTAAAAAG CCACCCATCC CACCTCCAGT GCCTGCTAAA 
AAGTCCAAAG AACGTTTGCC GAATGGTCTG CGACACCCTC CGCTGGTGCT GTCCGGTTCA 
GTGCCAAACA CTCCAACCCT ACCCCCAAAG CCTATATATT CTCCGACCAC CCCCTCTCCC 
TCTGAGGGTA CCCCATCAAC CCCAGCATCA CCAGGTGAAT CTGCAGCACC TCTGAGCCCA 
CCGTGGCTGT CTGACCTGCC TGAATCAGCA TGTCCTCAAA TCCATGGAGC TAAAATCGCT 
CTCAGCAGGA AGATCTCCCA TGCCAAGATG CTTGACCTAG AGACTCTGCT GGAGGACAGG 
ATGCACTCGG AAGGGATCGA CCTCAAAGAG GAGCCTTACT CTGATAAACA TGGCCGGTGT 
GGTATTCCTC AGCTCTTAGT TCAGAGGTAC TCAGAGGACT TCCAGCAGCC AGCGAAGGAC 
GTGGCGTCCA GTCTTGATCA AATCAGAGTT AAACAACTCC GCAAGGAGCA CCGCATGGCT 
ATTCCCTCAG GAGGTTTGAC TGACATGTGT CGGAAGCCAG TGTCTCTGGT TCATGTGAGC 
TCTGTCTCTG ATTGGCTCGT GTCCATCGGA ATGCCTATGT ATTCTGCACC TCTACTGGCT 
GCTGGCTTTG ACACGTTGAG TCGTGTGTCG TCTTTAAATG AGGCGCAAGC GAGGGACGCG 
GGCCTGAAGG AGGAACATCA CATACGGATT CTTCTCAGTG AAGCTCAACT CGTCTCAGCC 
GCCAGCTCAG GACAGTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sash1a ( XM_021468436.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324111.1 Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa150626
Clone ID Related Accession (Same CDS sequence)	XM_021468436.1
Accession Version	XM_021468436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SAM and SH3 domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGCT CTCTGGGTAA TATAGATGAC CTGGCACAAG AGTACTCGGA GTACTACAAC 
ACATACTTCA GTGACGTCAG CGATCGGATG GAGGAACTGC GCAAACGGCA AGTCTCTCAA 
GAGCTAGACA TGGAAAAATC AGACACTAGT CTAAACTCTT CACAGCTCCG CTCAGACATC 
CAGGATTCAT TTGGATTCAG CAGTGAGGTG TCCACACCAG AGGCTGAGAG AAAAATTTCT 
CTGCACAAAT CCAGCTCTGA GGATGGATCA GGAAAATGGG ACAACAAGAA GAAAAACAAA 
TCCTTCTGGC AGAACTTCCG AAAGTCACAG AAGGGGGTCA CAAGGCAAGC GTCAAAAGGA 
GAAGACATTG GCTATGTGGC CAGTGAGATC ACAATGAGTG ATGAAGAGCG AATCCAGCTT 
ATGATGATGG TGAAAGAGAA GATGATCACT GTAGAGGAAG CTCTGTCACG GTCTGTGGAG 
CAGTCAGATG ATGAGCAGGA GGACTCTGTG AAATTCAAAA GGCTTCACAA GCTGGTGAAC 
TCCACTCGAC GTGTCCGCAA GAAACTCATC AAAGTGGATG ATAGCAAGAG ACATGGCTCA 
CAAGATTCAC TCAGTCTGGA TGCAGCTCCC ATATGCGACG ATATAAATGC ACTATATGCA 
GAAATCCACA AGAAGCCGGC AAAGTGTACA GACTCCTCTT TGTCCTCACT TGCCCAAGAG 
CAGCTTTCTC TGGACGGGGA CACGGACAGC CTGAACACCT CGCCCTCCAC CAGCAGCCTT 
GATGCCTGGA AACCTGCTCA CAAATTAGTC AAGGCTCCCA GCACACATCC CCATGGCCTG 
ATCCGGCCAG CACGCAAGAG CAGTGCTGGG TCTGGGCTGG GTGTAGCGGG GGGCAGTGGC 
TCCTCGTTTT CTGAATTGGA CGGTTTGGGA GACGACAATG CTAAAGTCTC CCGCTCGGTT 
ACAGACGGAG AGATGCGGAA GGCCTTGTCA TCTCTATCTC ATGGGGTAAG TACTGATAGC 
GTTTACGCTT TTTATGGCCT CACTAGGCCC CGCCCAAAGC CACACCCCCT CCTGGTGTCT 
TTAGATGACA TCAACAACAC CAAGCGGCCA CCTGTTTCCA TGTGGCAGCG AAATAAGCCT 
GACCCGAACT ATGCATACTC TACCAAACAC CTGCTTTACC AACGTTCCTG CAACGCTCCG 
AAAACCGCAC CCTCCTCCAC CCTACCTGCG TCTCCTCCCA CTCGCGAGCT TCCGCTAGCC 
AAGGAGAAGG GGAAGAGTTG TGGGAGCGGG GTAGTGGGCA GCTGGCTGTT TCCACCAAGG 
CGTCTCAAAG GAAGGACGGC AGTTAGTGAG CTCAACATCA CATATGTGGT GGAACGCAGC 
CTGTACGGAC ATTTGAACTG GACTGGGCTG GTCAGGCCAA TCACATTGAG CAAAGCGGAC 
AGACGTTGGC TCCTGGAGGA TGAAAGAGAT GCCAACAGGA AATGGGCATC TAGCATGGAC 
CGCTGCACCA AGCGTGTGCT CTTGCGCATC CAACAAAAGT CTAGAACATG TAGCTTTGGA 
GGATTTGACC TATCTAATCG TTCCCTCCAT GTGGTCAACA CAGCAGAGCC TAGCGAGCAG 
GAGGCTCTGT ACAGGGATGG CGTGAAATCT CCCACCACAT CCCGTATCTC TCTAGGAAAG 
AAAGTCAAGT CTGTCAAAGA GACTATGAGG AAACGCATGT CCAAGAAATA TGTCGGGTCT 
CTCTCTGAAC AGTCCAGTCC AGATGGGACT CCCAGTTGTC CTCAGTCTCC TCAACCAGAC 
ACAGATTCTC TAGAGAAGCC CAAGCTCAAG GCAGGAGGGT CTGTGGAGAG TCTGCGCAGC 
TCTCTCAGTG GCCAAAGCTC CATGAGTGGG CAGACGGTGA GCACCACAGA CTCATCAGCC 
AGTAACAGAG AGAGTGTGAG GAGTGAGGAT GGTGATGATG AAGAGCCTCC ATACAGAGGC 
CCGTTCTGTG GCAGAGCAAG AGTTCACACT GACTTCACAC CCAGTCCATA TGATACAGAT 
TCTCTCAAGC TCAAGAGAGG AGATGTGATT GACATCATCA GTAAGCCGCC CATGGGCACA 
TGGATGGGTC TGCTCAATAA CAAAGTGGGC ACGTTTAAGT TCATCTATGT GGATGTTCTG 
GCAGAAGAAG AGGAGAAGCC CAAACGAGCT GTTCGCAGGA GGAAGAAGGG CCGACCACCC 
AAACCCAGCT CAGTCGAGGA GCTTCTGGAG AGAATCAACC TGAAGGAGCA CATGCCAACG 
TTTCTGTTTA ACGGGTATGA AGATCTGGAC ACGTTTAAGT TACTAGAGGA AGAAGATCTG 
GATGAGCTCA ACATCAAAGA CCCACAACAC CGAGCCGTAC TGATGACAGC TGTTGAACTG 
CTTCAGGATT ATGACAGCAG CAGTGATCCA GAGCGCAGCG GTCTCTCTGG CTCTCAGGAG 
AAGCTCCTCT GTGAGGGCCA TGGCCTCATG GCTGACTCTC CACGGGACTC TGGTTGCTAC 
GAAAGCAATG AAAACCTAGA GAATGGTAAG AGCCGAAAGG CATCGCGCCA CAGCCGTGGT 
TCATCAGGGT TTGACTGTGG GGATGTAAAG TCTCCAGATT ATCCCACTCT TCCCATGACT 
CGCTCCACTG AAGCTCTTCA ACAGCAGGCC AAGAAGGAGC GCAAGTTCTC GAAGACCTTT 
CTGCCCCGAC CCATGAAGGG CTTCAATCTG CGAAACCTCG GCCTCAAGAA AGGCTCCGCA 
GCAGCTCAAA CCTCCCGGAT TTCGCTCAGC AGAAGCTGTG AGGAGTTGGA CAGCCCCCAG 
GAGACCCCTG ACTCTTGGAA GCGCTCTCAT TCTCTGGGGG ATATCCACTG GGAACAGGCC 
TTCGATCAGA GGAAGGACCA GCAGGGCAGT GAGGATAGAG GCAGGCTGGA GAGCAGCACA 
AAAGAAATGA AAGCAGATCT TGTAGACTCC AAACAGGATG AGAAGACCAA TATGGCTAAA 
AGGCAGACTG GCTCCTTGCA GAGGCCTGCA GTTCCCACAC AGCTTCCTGT GAGTCCGGTC 
AGTTCATTGC CTGCTTCTCA GAGCTTATTC CGTCAACCTG TCTCTGCTCC GACAAGCCCA 
GCTCCAAGGA CTCACCCTAA AAAGCCACCC ATCCCACCTC CAGTGCCTGC TAAAAAGTCC 
AAAGAACGTT TGCCGAATGG TCTGCGACAC CCTCCGCTGG TGCTGTCCGG TTCAGTGCCA 
AACACTCCAA CCCTACCCCC AAAGCCTATA TATTCTCCGA CCACCCCCTC TCCCTCTGAG 
GGTACCCCAT CAACCCCAGC ATCACCAGGT GAATCTGCAG CACCTCTGAG CCCACCGTGG 
CTGTCTGACC TGCCTGAATC AGCATGTCCT CAAATCCATG GAGCTAAAAT CGCTCTCAGC 
AGGAAGATCT CCCATGCCAA GATGCTTGAC CTAGAGACTC TGCTGGAGGA CAGGATGCAC 
TCGGAAGGGA TCGACCTCAA AGAGGAGCCT TACTCTGATA AACATGGCCG GTGTGGTATT 
CCTCAGCTCT TAGTTCAGAG GTACTCAGAG GACTTCCAGC AGCCAGCGAA GGACGTGGCG 
TCCAGTCTTG ATCAAATCAG AGTTAAACAA CTCCGCAAGG AGCACCGCAT GGCTATTCCC 
TCAGGAGGTT TGACTGACAT GTGTCGGAAG CCAGTGTCTC TGGTTCATGT GAGCTCTGTC 
TCTGATTGGC TCGTGTCCAT CGGAATGCCT ATGTATTCTG CACCTCTACT GGCTGCTGGC 
TTTGACACGT TGAGTCGTGT GTCGTCTTTA AATGAGGCGC AAGCGAGGGA CGCGGGCCTG 
AAGGAGGAAC ATCACATACG GATTCTTCTC AGTGAAGCTC AACTCGTCTC AGCCGCCAGC 
TCAGGACAGT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324111.1
CDS	175..4089
Translation	MDGSLGNIDDLAQEYSEYYNTYFSDVSDRMEELRKRQVSQELDMEKSDTSLNSSQLRSDIQDSFGFSSEVSTPEAERKISLHKSSSEDGSGKWDNKKKNKSFWQNFRKSQKGVTRQASKGEDIGYVASEITMSDEERIQLMMMVKEKMITVEEALSRSVEQSDDEQEDSVKFKRLHKLVNSTRRVRKKLIKVDDSKRHGSQDSLSLDAAPICDDINALYAEIHKKPAKCTDSSLSSLAQEQLSLDGDTDSLNTSPSTSSLDAWKPAHKLVKAPSTHPHGLIRPARKSSAGSGLGVAGGSGSSFSELDGLGDDNAKVSRSVTDGEMRKALSSLSHGVSTDSVYAFYGLTRPRPKPHPLLVSLDDINNTKRPPVSMWQRNKPDPNYAYSTKHLLYQRSCNAPKTAPSSTLPASPPTRELPLAKEKGKSCGSGVVGSWLFPPRRLKGRTAVSELNITYVVERSLYGHLNWTGLVRPITLSKADRRWLLEDERDANRKWASSMDRCTKRVLLRIQQKSRTCSFGGFDLSNRSLHVVNTAEPSEQEALYRDGVKSPTTSRISLGKKVKSVKETMRKRMSKKYVGSLSEQSSPDGTPSCPQSPQPDTDSLEKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSASNRESVRSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKRGDVIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLAEEEEKPKRAVRRRKKGRPPKPSSVEELLERINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIKDPQHRAVLMTAVELLQDYDSSSDPERSGLSGSQEKLLCEGHGLMADSPRDSGCYESNENLENGKSRKASRHSRGSSGFDCGDVKSPDYPTLPMTRSTEALQQQAKKERKFSKTFLPRPMKGFNLRNLGLKKGSAAAQTSRISLSRSCEELDSPQETPDSWKRSHSLGDIHWEQAFDQRKDQQGSEDRGRLESSTKEMKADLVDSKQDEKTNMAKRQTGSLQRPAVPTQLPVSPVSSLPASQSLFRQPVSAPTSPAPRTHPKKPPIPPPVPAKKSKERLPNGLRHPPLVLSGSVPNTPTLPPKPIYSPTTPSPSEGTPSTPASPGESAAPLSPPWLSDLPESACPQIHGAKIALSRKISHAKMLDLETLLEDRMHSEGIDLKEEPYSDKHGRCGIPQLLVQRYSEDFQQPAKDVASSLDQIRVKQLRKEHRMAIPSGGLTDMCRKPVSLVHVSSVSDWLVSIGMPMYSAPLLAAGFDTLSRVSSLNEAQARDAGLKEEHHIRILLSEAQLVSAASSGQS

Target ORF information:

RefSeq Version	XM_021468436.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468436.1

ORF Insert Sequence:

ATGGATGGCT CTCTGGGTAA TATAGATGAC CTGGCACAAG AGTACTCGGA GTACTACAAC 
ACATACTTCA GTGACGTCAG CGATCGGATG GAGGAACTGC GCAAACGGCA AGTCTCTCAA 
GAGCTAGACA TGGAAAAATC AGACACTAGT CTAAACTCTT CACAGCTCCG CTCAGACATC 
CAGGATTCAT TTGGATTCAG CAGTGAGGTG TCCACACCAG AGGCTGAGAG AAAAATTTCT 
CTGCACAAAT CCAGCTCTGA GGATGGATCA GGAAAATGGG ACAACAAGAA GAAAAACAAA 
TCCTTCTGGC AGAACTTCCG AAAGTCACAG AAGGGGGTCA CAAGGCAAGC GTCAAAAGGA 
GAAGACATTG GCTATGTGGC CAGTGAGATC ACAATGAGTG ATGAAGAGCG AATCCAGCTT 
ATGATGATGG TGAAAGAGAA GATGATCACT GTAGAGGAAG CTCTGTCACG GTCTGTGGAG 
CAGTCAGATG ATGAGCAGGA GGACTCTGTG AAATTCAAAA GGCTTCACAA GCTGGTGAAC 
TCCACTCGAC GTGTCCGCAA GAAACTCATC AAAGTGGATG ATAGCAAGAG ACATGGCTCA 
CAAGATTCAC TCAGTCTGGA TGCAGCTCCC ATATGCGACG ATATAAATGC ACTATATGCA 
GAAATCCACA AGAAGCCGGC AAAGTGTACA GACTCCTCTT TGTCCTCACT TGCCCAAGAG 
CAGCTTTCTC TGGACGGGGA CACGGACAGC CTGAACACCT CGCCCTCCAC CAGCAGCCTT 
GATGCCTGGA AACCTGCTCA CAAATTAGTC AAGGCTCCCA GCACACATCC CCATGGCCTG 
ATCCGGCCAG CACGCAAGAG CAGTGCTGGG TCTGGGCTGG GTGTAGCGGG GGGCAGTGGC 
TCCTCGTTTT CTGAATTGGA CGGTTTGGGA GACGACAATG CTAAAGTCTC CCGCTCGGTT 
ACAGACGGAG AGATGCGGAA GGCCTTGTCA TCTCTATCTC ATGGGGTAAG TACTGATAGC 
GTTTACGCTT TTTATGGCCT CACTAGGCCC CGCCCAAAGC CACACCCCCT CCTGGTGTCT 
TTAGATGACA TCAACAACAC CAAGCGGCCA CCTGTTTCCA TGTGGCAGCG AAATAAGCCT 
GACCCGAACT ATGCATACTC TACCAAACAC CTGCTTTACC AACGTTCCTG CAACGCTCCG 
AAAACCGCAC CCTCCTCCAC CCTACCTGCG TCTCCTCCCA CTCGCGAGCT TCCGCTAGCC 
AAGGAGAAGG GGAAGAGTTG TGGGAGCGGG GTAGTGGGCA GCTGGCTGTT TCCACCAAGG 
CGTCTCAAAG GAAGGACGGC AGTTAGTGAG CTCAACATCA CATATGTGGT GGAACGCAGC 
CTGTACGGAC ATTTGAACTG GACTGGGCTG GTCAGGCCAA TCACATTGAG CAAAGCGGAC 
AGACGTTGGC TCCTGGAGGA TGAAAGAGAT GCCAACAGGA AATGGGCATC TAGCATGGAC 
CGCTGCACCA AGCGTGTGCT CTTGCGCATC CAACAAAAGT CTAGAACATG TAGCTTTGGA 
GGATTTGACC TATCTAATCG TTCCCTCCAT GTGGTCAACA CAGCAGAGCC TAGCGAGCAG 
GAGGCTCTGT ACAGGGATGG CGTGAAATCT CCCACCACAT CCCGTATCTC TCTAGGAAAG 
AAAGTCAAGT CTGTCAAAGA GACTATGAGG AAACGCATGT CCAAGAAATA TGTCGGGTCT 
CTCTCTGAAC AGTCCAGTCC AGATGGGACT CCCAGTTGTC CTCAGTCTCC TCAACCAGAC 
ACAGATTCTC TAGAGAAGCC CAAGCTCAAG GCAGGAGGGT CTGTGGAGAG TCTGCGCAGC 
TCTCTCAGTG GCCAAAGCTC CATGAGTGGG CAGACGGTGA GCACCACAGA CTCATCAGCC 
AGTAACAGAG AGAGTGTGAG GAGTGAGGAT GGTGATGATG AAGAGCCTCC ATACAGAGGC 
CCGTTCTGTG GCAGAGCAAG AGTTCACACT GACTTCACAC CCAGTCCATA TGATACAGAT 
TCTCTCAAGC TCAAGAGAGG AGATGTGATT GACATCATCA GTAAGCCGCC CATGGGCACA 
TGGATGGGTC TGCTCAATAA CAAAGTGGGC ACGTTTAAGT TCATCTATGT GGATGTTCTG 
GCAGAAGAAG AGGAGAAGCC CAAACGAGCT GTTCGCAGGA GGAAGAAGGG CCGACCACCC 
AAACCCAGCT CAGTCGAGGA GCTTCTGGAG AGAATCAACC TGAAGGAGCA CATGCCAACG 
TTTCTGTTTA ACGGGTATGA AGATCTGGAC ACGTTTAAGT TACTAGAGGA AGAAGATCTG 
GATGAGCTCA ACATCAAAGA CCCACAACAC CGAGCCGTAC TGATGACAGC TGTTGAACTG 
CTTCAGGATT ATGACAGCAG CAGTGATCCA GAGCGCAGCG GTCTCTCTGG CTCTCAGGAG 
AAGCTCCTCT GTGAGGGCCA TGGCCTCATG GCTGACTCTC CACGGGACTC TGGTTGCTAC 
GAAAGCAATG AAAACCTAGA GAATGGTAAG AGCCGAAAGG CATCGCGCCA CAGCCGTGGT 
TCATCAGGGT TTGACTGTGG GGATGTAAAG TCTCCAGATT ATCCCACTCT TCCCATGACT 
CGCTCCACTG AAGCTCTTCA ACAGCAGGCC AAGAAGGAGC GCAAGTTCTC GAAGACCTTT 
CTGCCCCGAC CCATGAAGGG CTTCAATCTG CGAAACCTCG GCCTCAAGAA AGGCTCCGCA 
GCAGCTCAAA CCTCCCGGAT TTCGCTCAGC AGAAGCTGTG AGGAGTTGGA CAGCCCCCAG 
GAGACCCCTG ACTCTTGGAA GCGCTCTCAT TCTCTGGGGG ATATCCACTG GGAACAGGCC 
TTCGATCAGA GGAAGGACCA GCAGGGCAGT GAGGATAGAG GCAGGCTGGA GAGCAGCACA 
AAAGAAATGA AAGCAGATCT TGTAGACTCC AAACAGGATG AGAAGACCAA TATGGCTAAA 
AGGCAGACTG GCTCCTTGCA GAGGCCTGCA GTTCCCACAC AGCTTCCTGT GAGTCCGGTC 
AGTTCATTGC CTGCTTCTCA GAGCTTATTC CGTCAACCTG TCTCTGCTCC GACAAGCCCA 
GCTCCAAGGA CTCACCCTAA AAAGCCACCC ATCCCACCTC CAGTGCCTGC TAAAAAGTCC 
AAAGAACGTT TGCCGAATGG TCTGCGACAC CCTCCGCTGG TGCTGTCCGG TTCAGTGCCA 
AACACTCCAA CCCTACCCCC AAAGCCTATA TATTCTCCGA CCACCCCCTC TCCCTCTGAG 
GGTACCCCAT CAACCCCAGC ATCACCAGGT GAATCTGCAG CACCTCTGAG CCCACCGTGG 
CTGTCTGACC TGCCTGAATC AGCATGTCCT CAAATCCATG GAGCTAAAAT CGCTCTCAGC 
AGGAAGATCT CCCATGCCAA GATGCTTGAC CTAGAGACTC TGCTGGAGGA CAGGATGCAC 
TCGGAAGGGA TCGACCTCAA AGAGGAGCCT TACTCTGATA AACATGGCCG GTGTGGTATT 
CCTCAGCTCT TAGTTCAGAG GTACTCAGAG GACTTCCAGC AGCCAGCGAA GGACGTGGCG 
TCCAGTCTTG ATCAAATCAG AGTTAAACAA CTCCGCAAGG AGCACCGCAT GGCTATTCCC 
TCAGGAGGTT TGACTGACAT GTGTCGGAAG CCAGTGTCTC TGGTTCATGT GAGCTCTGTC 
TCTGATTGGC TCGTGTCCAT CGGAATGCCT ATGTATTCTG CACCTCTACT GGCTGCTGGC 
TTTGACACGT TGAGTCGTGT GTCGTCTTTA AATGAGGCGC AAGCGAGGGA CGCGGGCCTG 
AAGGAGGAAC ATCACATACG GATTCTTCTC AGTGAAGCTC AACTCGTCTC AGCCGCCAGC 
TCAGGACAGT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sash1a ( NM_001044819.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038284.1 Danio rerio SAM and SH3 domain containing 1a (sash1a), mRNA.

Price & Availability↑

CloneID	ODa34904
Clone ID Related Accession (Same CDS sequence)	NM_001044819.1
Accession Version	NM_001044819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	SAM and SH3 domain-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX511140.4. On Sep 1, 2006 this sequence version replaced XM_680137.1. ##Evidence-Data-START## Transcript exon combination :: BC171610.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGAGG ATCAAGTGTC TTCGGCCGAG GAGCCGCAGG CGGTCAGCGC CGCATCTTCG 
GACAGTTTCT CCCGGCTCTG GACGGACGTG ATGGGGATGC TGGATGGCTC TCTGGGTAAT 
ATAGATGACC TGGCACAAGA GTACTCGGAG TACTACAACA CATACTTCAG TGACGTCAGC 
GATCGGATGG AGGAACTGCG CAAACGGCAA GTCTCTCAAG AGCTAGACAT GGAAAAATCA 
GACACTAGTC TAAACTCTTC ACAGCTCCGC TCAGACATCC AGGATTCATT TGGATTCAGC 
AGTGAGGTGT CCACACCAGA GGCTGAGAGA AAAATTTCTC TGCACAAATC CAGCTCTGAG 
GATGGATCAG GAAAATGGGA CAACAAGAAG AAAAACAAAT CCTTCTGGCA GAACTTCCGA 
AAGTCACAGA AGGGGGTCAC AAGGCAAGCG TCAAAAGGAG AAGACATTGG CTATGTGGCC 
AGTGAGATCA CAATGAGTGA TGAAGAGCGA ATCCAGCTTA TGATGATGGT GAAAGAGAAG 
ATGATCACTG TAGAGGAAGC TCTGTCACGG TTAAAGGAAT ATGAGAGGAA CAGGCAGTTG 
AGCAGCAGTA CAGACACAGC AGAGTGGACT GATGGATCCA GTCCCACTGT TAACCTCTCC 
TCCACCTGCA ATTCTGTGGA GCAGTCAGAT GATGAGCAGG AGGACTCTGT GAAATTCAAA 
AGGCTTCACA AGCTGGTGAA CTCCACTCGA CGTGTCCGCA AGAAACTCAT CAAAGTGGAT 
GATAGCAAGA GACATGGCTC ACAAGATTCA CTCAGTCTGG ATGCAGCTCC CATATGCGAC 
GATATAAATG CACTATATGC AGAAATCCAC AAGAAGCCGG CAAAGTGTAC AGACTCCTCT 
TTGTCCTCAC TTGCCCAAGA GCAGCTTTCT CTGGACGGGG ACACGGACAG CCTGAACACC 
TCGCCCTCCA CCAGCAGCCT TGATGCCTGG AAACCTGCTC ACAAATTAGT CAAGGCTCCC 
AGCACACATC CCCATGGCCT GATCCGGCCA GCACGCAAGA GCAGTGCTGG GTCTGGGCTG 
GGTGTAGCGG GGGGCAGTGG CTCCTCGTTT TCTGAATTGG ACGGTTTGGG AGACGACAAT 
GCTAAAGTCT CCCGCTCGGT TACAGACGGA GAGATGCGGA AGGCCTTGTC ATCTCTATCT 
CATGGGAGAA CATGTAGCTT TGGAGGATTT GACCTATCTA ATCGTTCCCT CCATGTGGTC 
AACACAGCAG AGCCTAGCGA GCAGGAGGCT CTGTACAGGG ATGGCGTGAA ATCTCCCACC 
ACATCCCGTA TCTCTCTAGG AAAGAAAGTC AAGTCTGTCA AAGAGACTAT GAGGAAACGC 
ATGTCCAAGA AATATGTCGG GTCTCTCTCT GAACAGTCCA GTCCAGATGG GACTCCCAGT 
TGTCCTCAGT CTCCTCAACC AGACACAGAT TCTCTAGAGA AGCCCAAGCT CAAGGCAGGA 
GGGTCTGTGG AGAGTCTGCG CAGCTCTCTC AGTGGCCAAA GCTCCATGAG TGGGCAGACG 
GTGAGCACCA CAGACTCATC AGCCAGTAAC AGAGAGAGTG TGAGGAGTGA GGATGGTGAT 
GATGAAGAGC CTCCATACAG AGGCCCGTTC TGTGGCAGAG CAAGAGTTCA CACTGACTTC 
ACACCCAGTC CATATGATAC AGATTCTCTC AAGCTCAAGA GAGGAGATGT GATTGACATC 
ATCAGTAAGC CGCCCATGGG CACATGGATG GGTCTGCTCA ATAACAAAGT GGGCACGTTT 
AAGTTCATCT ATGTGGATGT TCTGGCAGAA GAAGAGGAGA AGCCCAAACG AGCTGTTCGC 
AGGAGGAAGA AGGGCCGACC ACCCAAACCC AGCTCAGTCG AGGAGCTTCT GGAGAGAATC 
AACCTGAAGG AGCACATGCC AACGTTTCTG TTTAACGGGT ATGAAGATCT GGACACGTTT 
AAGTTACTAG AGGAAGAAGA TCTGGATGAG CTCAACATCA AAGACCCACA ACACCGAGCC 
GTACTGATGA CAGCTGTTGA ACTGCTTCAG GATTATGACA GCAGCAGTGA TCCAGAGCGC 
AGCGGTCTCT CTGGCTCTCA GGAGAAGCTC CTCTGTGAGG GCCATGGCCT CATGGCTGAC 
TCTCCACGGG ACTCTGGTTG CTACGAAAGC AATGAAAACC TAGAGAATGG TAAGAGCCGA 
AAGGCATCGC GCCACAGCCG TGGTTCATCA GGGTTTGACT GTGGGGATGT AAAGTCTCCA 
GATTATCCCA CTCTTCCCAT GACTCGCTCC ACTGAAGCTC TTCAACAGCA GGCCAAGAAG 
GAGCGCAAGT TCTCGAAGAC CTTTCTGCCC CGACCCATGA AGGGCTTCAA TCTGCGAAAC 
CTCGGCCTCA AGAAAGGCTC CGCAGCAGCT CAAACCTCCC GGATTTCGCT CAGCAGAAGC 
TGTGAGGAGT TGGACAGCCC CCAGGAGACC CCTGACTCTT GGAAGCGCTC TCATTCTCTG 
GGGGATATCC ACTGGGAACA GGCCTTCGAT CAGAGGAAGG ACCAGCAGGG CAGTGAGGAT 
AGAGGCAGGC TGGAGAGCAG CACAAAAGAA ATGAAAGCAG ATCTTGTAGA CTCCAAACAG 
GATGAGAAGA CCAATATGGC TAAAAGGCAG ACTGGCTCCT TGCAGAGGCC TGCAGTTCCC 
ACACAGCTTC CTGTGAGTCC GGTCAGTTCA TTGCCTGCTT CTCAGAGCTT ATTCCGTCAA 
CCTGTCTCTG CTCCGACAAG CCCAGCTCCA AGGACTCACC CTAAAAAGCC ACCCATCCCA 
CCTCCAGTGC CTGCTAAAAA GTCCAAAGAA CGTTTGCCGA ATGGTCTGCG ACACCCTCCG 
CTGGTGCTGT CCGGTTCAGT GCCAAACACT CCAACCCTAC CCCCAAAGCC TATATATTCT 
CCGACCACCC CCTCTCCCTC TGAGGGTACC CCATCAACCC CAGCATCACC AGGTGAATCT 
GCAGCACCTC TGAGCCCACC GTGGCTGTCT GACCTGCCTG AATCAGCATG TCCTCAAATC 
CATGGAGCTA AAATCGCTCT CAGCAGGAAG ATCTCCCATG CCAAGATGCT TGACCTAGAG 
ACTCTGCTGG AGGACAGGAT GCACTCGGAA GGGATCGACC TCAAAGAGGA GCCTTACTCT 
GATAAACATG GCCGGTGTGG TATTCCTCAG CTCTTAGTTC AGAGGTACTC AGAGGACTTC 
CAGCAGCCAG CGAAGGACGT GGCGTCCAGT CTTGATCAAA TCAGAGTTAA ACAACTCCGC 
AAGGAGCACC GCATGGCTAT TCCCTCAGGA GGTTTGACTG ACATGTGTCG GAAGCCAGTG 
TCTCTGGTTC ATGTGAGCTC TGTCTCTGAT TGGCTCGTGT CCATCGGAAT GCCTATGTAT 
TCTGCACCTC TACTGGCTGC TGGCTTTGAC ACGTTGAGTC GTGTGTCGTC TTTAAATGAG 
GCGCAAGCGA GGGACGCGGG CCTGAAGGAG GAACATCACA TACGGATTCT TCTCAGTGAA 
GCTCAACTCG TCTCAGCCGC CAGCTCAGGA CAGTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038284.1
CDS	444..4022
Translation	MEEDQVSSAEEPQAVSAASSDSFSRLWTDVMGMLDGSLGNIDDLAQEYSEYYNTYFSDVSDRMEELRKRQVSQELDMEKSDTSLNSSQLRSDIQDSFGFSSEVSTPEAERKISLHKSSSEDGSGKWDNKKKNKSFWQNFRKSQKGVTRQASKGEDIGYVASEITMSDEERIQLMMMVKEKMITVEEALSRLKEYERNRQLSSSTDTAEWTDGSSPTVNLSSTCNSVEQSDDEQEDSVKFKRLHKLVNSTRRVRKKLIKVDDSKRHGSQDSLSLDAAPICDDINALYAEIHKKPAKCTDSSLSSLAQEQLSLDGDTDSLNTSPSTSSLDAWKPAHKLVKAPSTHPHGLIRPARKSSAGSGLGVAGGSGSSFSELDGLGDDNAKVSRSVTDGEMRKALSSLSHGRTCSFGGFDLSNRSLHVVNTAEPSEQEALYRDGVKSPTTSRISLGKKVKSVKETMRKRMSKKYVGSLSEQSSPDGTPSCPQSPQPDTDSLEKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSASNRESVRSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKRGDVIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLAEEEEKPKRAVRRRKKGRPPKPSSVEELLERINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIKDPQHRAVLMTAVELLQDYDSSSDPERSGLSGSQEKLLCEGHGLMADSPRDSGCYESNENLENGKSRKASRHSRGSSGFDCGDVKSPDYPTLPMTRSTEALQQQAKKERKFSKTFLPRPMKGFNLRNLGLKKGSAAAQTSRISLSRSCEELDSPQETPDSWKRSHSLGDIHWEQAFDQRKDQQGSEDRGRLESSTKEMKADLVDSKQDEKTNMAKRQTGSLQRPAVPTQLPVSPVSSLPASQSLFRQPVSAPTSPAPRTHPKKPPIPPPVPAKKSKERLPNGLRHPPLVLSGSVPNTPTLPPKPIYSPTTPSPSEGTPSTPASPGESAAPLSPPWLSDLPESACPQIHGAKIALSRKISHAKMLDLETLLEDRMHSEGIDLKEEPYSDKHGRCGIPQLLVQRYSEDFQQPAKDVASSLDQIRVKQLRKEHRMAIPSGGLTDMCRKPVSLVHVSSVSDWLVSIGMPMYSAPLLAAGFDTLSRVSSLNEAQARDAGLKEEHHIRILLSEAQLVSAASSGQS

Target ORF information:

RefSeq Version	NM_001044819.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SAM and SH3 domain containing 1a (sash1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044819.1

ORF Insert Sequence:

ATGGAAGAGG ATCAAGTGTC TTCGGCCGAG GAGCCGCAGG CGGTCAGCGC CGCATCTTCG 
GACAGTTTCT CCCGGCTCTG GACGGACGTG ATGGGGATGC TGGATGGCTC TCTGGGTAAT 
ATAGATGACC TGGCACAAGA GTACTCGGAG TACTACAACA CATACTTCAG TGACGTCAGC 
GATCGGATGG AGGAACTGCG CAAACGGCAA GTCTCTCAAG AGCTAGACAT GGAAAAATCA 
GACACTAGTC TAAACTCTTC ACAGCTCCGC TCAGACATCC AGGATTCATT TGGATTCAGC 
AGTGAGGTGT CCACACCAGA GGCTGAGAGA AAAATTTCTC TGCACAAATC CAGCTCTGAG 
GATGGATCAG GAAAATGGGA CAACAAGAAG AAAAACAAAT CCTTCTGGCA GAACTTCCGA 
AAGTCACAGA AGGGGGTCAC AAGGCAAGCG TCAAAAGGAG AAGACATTGG CTATGTGGCC 
AGTGAGATCA CAATGAGTGA TGAAGAGCGA ATCCAGCTTA TGATGATGGT GAAAGAGAAG 
ATGATCACTG TAGAGGAAGC TCTGTCACGG TTAAAGGAAT ATGAGAGGAA CAGGCAGTTG 
AGCAGCAGTA CAGACACAGC AGAGTGGACT GATGGATCCA GTCCCACTGT TAACCTCTCC 
TCCACCTGCA ATTCTGTGGA GCAGTCAGAT GATGAGCAGG AGGACTCTGT GAAATTCAAA 
AGGCTTCACA AGCTGGTGAA CTCCACTCGA CGTGTCCGCA AGAAACTCAT CAAAGTGGAT 
GATAGCAAGA GACATGGCTC ACAAGATTCA CTCAGTCTGG ATGCAGCTCC CATATGCGAC 
GATATAAATG CACTATATGC AGAAATCCAC AAGAAGCCGG CAAAGTGTAC AGACTCCTCT 
TTGTCCTCAC TTGCCCAAGA GCAGCTTTCT CTGGACGGGG ACACGGACAG CCTGAACACC 
TCGCCCTCCA CCAGCAGCCT TGATGCCTGG AAACCTGCTC ACAAATTAGT CAAGGCTCCC 
AGCACACATC CCCATGGCCT GATCCGGCCA GCACGCAAGA GCAGTGCTGG GTCTGGGCTG 
GGTGTAGCGG GGGGCAGTGG CTCCTCGTTT TCTGAATTGG ACGGTTTGGG AGACGACAAT 
GCTAAAGTCT CCCGCTCGGT TACAGACGGA GAGATGCGGA AGGCCTTGTC ATCTCTATCT 
CATGGGAGAA CATGTAGCTT TGGAGGATTT GACCTATCTA ATCGTTCCCT CCATGTGGTC 
AACACAGCAG AGCCTAGCGA GCAGGAGGCT CTGTACAGGG ATGGCGTGAA ATCTCCCACC 
ACATCCCGTA TCTCTCTAGG AAAGAAAGTC AAGTCTGTCA AAGAGACTAT GAGGAAACGC 
ATGTCCAAGA AATATGTCGG GTCTCTCTCT GAACAGTCCA GTCCAGATGG GACTCCCAGT 
TGTCCTCAGT CTCCTCAACC AGACACAGAT TCTCTAGAGA AGCCCAAGCT CAAGGCAGGA 
GGGTCTGTGG AGAGTCTGCG CAGCTCTCTC AGTGGCCAAA GCTCCATGAG TGGGCAGACG 
GTGAGCACCA CAGACTCATC AGCCAGTAAC AGAGAGAGTG TGAGGAGTGA GGATGGTGAT 
GATGAAGAGC CTCCATACAG AGGCCCGTTC TGTGGCAGAG CAAGAGTTCA CACTGACTTC 
ACACCCAGTC CATATGATAC AGATTCTCTC AAGCTCAAGA GAGGAGATGT GATTGACATC 
ATCAGTAAGC CGCCCATGGG CACATGGATG GGTCTGCTCA ATAACAAAGT GGGCACGTTT 
AAGTTCATCT ATGTGGATGT TCTGGCAGAA GAAGAGGAGA AGCCCAAACG AGCTGTTCGC 
AGGAGGAAGA AGGGCCGACC ACCCAAACCC AGCTCAGTCG AGGAGCTTCT GGAGAGAATC 
AACCTGAAGG AGCACATGCC AACGTTTCTG TTTAACGGGT ATGAAGATCT GGACACGTTT 
AAGTTACTAG AGGAAGAAGA TCTGGATGAG CTCAACATCA AAGACCCACA ACACCGAGCC 
GTACTGATGA CAGCTGTTGA ACTGCTTCAG GATTATGACA GCAGCAGTGA TCCAGAGCGC 
AGCGGTCTCT CTGGCTCTCA GGAGAAGCTC CTCTGTGAGG GCCATGGCCT CATGGCTGAC 
TCTCCACGGG ACTCTGGTTG CTACGAAAGC AATGAAAACC TAGAGAATGG TAAGAGCCGA 
AAGGCATCGC GCCACAGCCG TGGTTCATCA GGGTTTGACT GTGGGGATGT AAAGTCTCCA 
GATTATCCCA CTCTTCCCAT GACTCGCTCC ACTGAAGCTC TTCAACAGCA GGCCAAGAAG 
GAGCGCAAGT TCTCGAAGAC CTTTCTGCCC CGACCCATGA AGGGCTTCAA TCTGCGAAAC 
CTCGGCCTCA AGAAAGGCTC CGCAGCAGCT CAAACCTCCC GGATTTCGCT CAGCAGAAGC 
TGTGAGGAGT TGGACAGCCC CCAGGAGACC CCTGACTCTT GGAAGCGCTC TCATTCTCTG 
GGGGATATCC ACTGGGAACA GGCCTTCGAT CAGAGGAAGG ACCAGCAGGG CAGTGAGGAT 
AGAGGCAGGC TGGAGAGCAG CACAAAAGAA ATGAAAGCAG ATCTTGTAGA CTCCAAACAG 
GATGAGAAGA CCAATATGGC TAAAAGGCAG ACTGGCTCCT TGCAGAGGCC TGCAGTTCCC 
ACACAGCTTC CTGTGAGTCC GGTCAGTTCA TTGCCTGCTT CTCAGAGCTT ATTCCGTCAA 
CCTGTCTCTG CTCCGACAAG CCCAGCTCCA AGGACTCACC CTAAAAAGCC ACCCATCCCA 
CCTCCAGTGC CTGCTAAAAA GTCCAAAGAA CGTTTGCCGA ATGGTCTGCG ACACCCTCCG 
CTGGTGCTGT CCGGTTCAGT GCCAAACACT CCAACCCTAC CCCCAAAGCC TATATATTCT 
CCGACCACCC CCTCTCCCTC TGAGGGTACC CCATCAACCC CAGCATCACC AGGTGAATCT 
GCAGCACCTC TGAGCCCACC GTGGCTGTCT GACCTGCCTG AATCAGCATG TCCTCAAATC 
CATGGAGCTA AAATCGCTCT CAGCAGGAAG ATCTCCCATG CCAAGATGCT TGACCTAGAG 
ACTCTGCTGG AGGACAGGAT GCACTCGGAA GGGATCGACC TCAAAGAGGA GCCTTACTCT 
GATAAACATG GCCGGTGTGG TATTCCTCAG CTCTTAGTTC AGAGGTACTC AGAGGACTTC 
CAGCAGCCAG CGAAGGACGT GGCGTCCAGT CTTGATCAAA TCAGAGTTAA ACAACTCCGC 
AAGGAGCACC GCATGGCTAT TCCCTCAGGA GGTTTGACTG ACATGTGTCG GAAGCCAGTG 
TCTCTGGTTC ATGTGAGCTC TGTCTCTGAT TGGCTCGTGT CCATCGGAAT GCCTATGTAT 
TCTGCACCTC TACTGGCTGC TGGCTTTGAC ACGTTGAGTC GTGTGTCGTC TTTAAATGAG 
GCGCAAGCGA GGGACGCGGG CCTGAAGGAG GAACATCACA TACGGATTCT TCTCAGTGAA 
GCTCAACTCG TCTCAGCCGC CAGCTCAGGA CAGTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sash1a ( XM_021468431.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324106.1 Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X1, mRNA.

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CloneID	ODa150621
Clone ID Related Accession (Same CDS sequence)	XM_021468431.1
Accession Version	XM_021468431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SAM and SH3 domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACTA ATGGACCCAA CATCGTCTAC GAATGGCTAA AAACCCTTCA ACTTTGCCAA 
TATGTCGAGG CGTTTGTGGA TAACGGATAC GACGACTTGG AAGTTTGCAA ACAAATCGGA 
GACCCTGACC TGGATGCCAT CGGGGTTTTC ATTCCGCACC ACCGCCAGCG GATTCATGAT 
GCGGTGCAAA GGTTAAAAGA TGACGATAGA GAGACGGCTA CCGGGCTTTA TTTCACCCTA 
GAGCCGATGC CCCCGGTCTC ACCTGTATAC ACGAGTCGCA TGGCAGAGCA ATATGAGTCA 
AGGCTGCGGG GCTCCAGGTC ATGGACAGAG CAGAGCAGTG ATAGAGCCGC ACGCGGCGTG 
GGGTACATGG GGGCGTCCAG GAATCTGACC CTGGGGAACC GCAAGGAGCT GGAAATCTAC 
CCCAAACTGA AGCTGAAGAT CATGATCAGG GATAAGCTCA TTAGGGACGG GATAAACCTT 
GCCAGGCAGC CATACTCTAA TAAGGATGGC TCTCTGGGTA ATATAGATGA CCTGGCACAA 
GAGTACTCGG AGTACTACAA CACATACTTC AGTGACGTCA GCGATCGGAT GGAGGAACTG 
CGCAAACGGC AAGTCTCTCA AGAGCTAGAC ATGGAAAAAT CAGACACTAG TCTAAACTCT 
TCACAGCTCC GCTCAGACAT CCAGGATTCA TTTGGATTCA GCAGTGAGGT GTCCACACCA 
GAGGCTGAGA GAAAAATTTC TCTGCACAAA TCCAGCTCTG AGGATGGATC AGGAAAATGG 
GACAACAAGA AGAAAAACAA ATCCTTCTGG CAGAACTTCC GAAAGTCACA GAAGGGGGTC 
ACAAGGCAAG CGTCAAAAGG AGAAGACATT GGCTATGTGG CCAGTGAGAT CACAATGAGT 
GATGAAGAGC GAATCCAGCT TATGATGATG GTGAAAGAGA AGATGATCAC TGTAGAGGAA 
GCTCTGTCAC GGTTAAAGGA ATATGAGAGG AACAGGCAGT TGAGCAGCAG TACAGACACA 
GCAGAGTGGA CTGATGGATC CAGTCCCACT GTTAACCTCT CCTCCACCTG CAATTCTGTG 
GAGCAGTCAG ATGATGAGCA GGAGGACTCT GTGAAATTCA AAAGGCTTCA CAAGCTGGTG 
AACTCCACTC GACGTGTCCG CAAGAAACTC ATCAAAGTGG ATGATAGCAA GAGACATGGC 
TCACAAGATT CACTCAGTCT GGATGCAGCT CCCATATGCG ACGATATAAA TGCACTATAT 
GCAGAAATCC ACAAGAAGCC GGCAAAGTGT ACAGACTCCT CTTTGTCCTC ACTTGCCCAA 
GAGCAGCTTT CTCTGGACGG GGACACGGAC AGCCTGAACA CCTCGCCCTC CACCAGCAGC 
CTTGATGCCT GGAAACCTGC TCACAAATTA GTCAAGGCTC CCAGCACACA TCCCCATGGC 
CTGATCCGGC CAGCACGCAA GAGCAGTGCT GGGTCTGGGC TGGGTGTAGC GGGGGGCAGT 
GGCTCCTCGT TTTCTGAATT GGACGGTTTG GGAGACGACA ATGCTAAAGT CTCCCGCTCG 
GTTACAGACG GAGAGATGCG GAAGGCCTTG TCATCTCTAT CTCATGGGGT AAGTACTGAT 
AGCGTTTACG CTTTTTATGG CCTCACTAGG CCCCGCCCAA AGCCACACCC CCTCCTGGTG 
TCTTTAGATG ACATCAACAA CACCAAGCGG CCACCTGTTT CCATGTGGCA GCGAAATAAG 
CCTGACCCGA ACTATGCATA CTCTACCAAA CACCTGCTTT ACCAACGTTC CTGCAACGCT 
CCGAAAACCG CACCCTCCTC CACCCTACCT GCGTCTCCTC CCACTCGCGA GCTTCCGCTA 
GCCAAGGAGA AGGGGAAGAG TTGTGGGAGC GGGGTAGTGG GCAGCTGGCT GTTTCCACCA 
AGGCGTCTCA AAGGAAGGAC GGCAGTTAGT GAGCTCAACA TCACATATGT GGTGGAACGC 
AGCCTGTACG GACATTTGAA CTGGACTGGG CTGGTCAGGC CAATCACATT GAGCAAAGCG 
GACAGACGTT GGCTCCTGGA GGATGAAAGA GATGCCAACA GGAAATGGGC ATCTAGCATG 
GACCGCTGCA CCAAGCGTGT GCTCTTGCGC ATCCAACAAA AGTCTAGAAC ATGTAGCTTT 
GGAGGATTTG ACCTATCTAA TCGTTCCCTC CATGTGGTCA ACACAGCAGA GCCTAGCGAG 
CAGGAGGCTC TGTACAGGGA TGGCGTGAAA TCTCCCACCA CATCCCGTAT CTCTCTAGGA 
AAGAAAGTCA AGTCTGTCAA AGAGACTATG AGGAAACGCA TGTCCAAGAA ATATGTCGGG 
TCTCTCTCTG AACAGTCCAG TCCAGATGGG ACTCCCAGTT GTCCTCAGTC TCCTCAACCA 
GACACAGATT CTCTAGAGAA GCCCAAGCTC AAGGCAGGAG GGTCTGTGGA GAGTCTGCGC 
AGCTCTCTCA GTGGCCAAAG CTCCATGAGT GGGCAGACGG TGAGCACCAC AGACTCATCA 
GCCAGTAACA GAGAGAGTGT GAGGAGTGAG GATGGTGATG ATGAAGAGCC TCCATACAGA 
GGCCCGTTCT GTGGCAGAGC AAGAGTTCAC ACTGACTTCA CACCCAGTCC ATATGATACA 
GATTCTCTCA AGCTCAAGAG AGGAGATGTG ATTGACATCA TCAGTAAGCC GCCCATGGGC 
ACATGGATGG GTCTGCTCAA TAACAAAGTG GGCACGTTTA AGTTCATCTA TGTGGATGTT 
CTGGCAGAAG AAGAGGAGAA GCCCAAACGA GCTGTTCGCA GGAGGAAGAA GGGCCGACCA 
CCCAAACCCA GCTCAGTCGA GGAGCTTCTG GAGAGAATCA ACCTGAAGGA GCACATGCCA 
ACGTTTCTGT TTAACGGGTA TGAAGATCTG GACACGTTTA AGTTACTAGA GGAAGAAGAT 
CTGGATGAGC TCAACATCAA AGACCCACAA CACCGAGCCG TACTGATGAC AGCTGTTGAA 
CTGCTTCAGG ATTATGACAG CAGCAGTGAT CCAGAGCGCA GCGGTCTCTC TGGCTCTCAG 
GAGAAGCTCC TCTGTGAGGG CCATGGCCTC ATGGCTGACT CTCCACGGGA CTCTGGTTGC 
TACGAAAGCA ATGAAAACCT AGAGAATGGT AAGAGCCGAA AGGCATCGCG CCACAGCCGT 
GGTTCATCAG GGTTTGACTG TGGGGATGTA AAGTCTCCAG ATTATCCCAC TCTTCCCATG 
ACTCGCTCCA CTGAAGCTCT TCAACAGCAG GCCAAGAAGG AGCGCAAGTT CTCGAAGACC 
TTTCTGCCCC GACCCATGAA GGGCTTCAAT CTGCGAAACC TCGGCCTCAA GAAAGGCTCC 
GCAGCAGCTC AAACCTCCCG GATTTCGCTC AGCAGAAGCT GTGAGGAGTT GGACAGCCCC 
CAGGAGACCC CTGACTCTTG GAAGCGCTCT CATTCTCTGG GGGATATCCA CTGGGAACAG 
GCCTTCGATC AGAGGAAGGA CCAGCAGGGC AGTGAGGATA GAGGCAGGCT GGAGAGCAGC 
ACAAAAGAAA TGAAAGCAGA TCTTGTAGAC TCCAAACAGG ATGAGAAGAC CAATATGGCT 
AAAAGGCAGA CTGGCTCCTT GCAGAGGCCT GCAGTTCCCA CACAGCTTCC TGTGAGTCCG 
GTCAGTTCAT TGCCTGCTTC TCAGAGCTTA TTCCGTCAAC CTGTCTCTGC TCCGACAAGC 
CCAGCTCCAA GGACTCACCC TAAAAAGCCA CCCATCCCAC CTCCAGTGCC TGCTAAAAAG 
TCCAAAGAAC GTTTGCCGAA TGGTCTGCGA CACCCTCCGC TGGTGCTGTC CGGTTCAGTG 
CCAAACACTC CAACCCTACC CCCAAAGCCT ATATATTCTC CGACCACCCC CTCTCCCTCT 
GAGGGTACCC CATCAACCCC AGCATCACCA GGTGAATCTG CAGCACCTCT GAGCCCACCG 
TGGCTGTCTG ACCTGCCTGA ATCAGCATGT CCTCAAATCC ATGGAGCTAA AATCGCTCTC 
AGCAGGAAGA TCTCCCATGC CAAGATGCTT GACCTAGAGA CTCTGCTGGA GGACAGGATG 
CACTCGGAAG GGATCGACCT CAAAGAGGAG CCTTACTCTG ATAAACATGG CCGGTGTGGT 
ATTCCTCAGC TCTTAGTTCA GAGGTACTCA GAGGACTTCC AGCAGCCAGC GAAGGACGTG 
GCGTCCAGTC TTGATCAAAT CAGAGTTAAA CAACTCCGCA AGGAGCACCG CATGGCTATT 
CCCTCAGGAG GTTTGACTGA CATGTGTCGG AAGCCAGTGT CTCTGGTTCA TGTGAGCTCT 
GTCTCTGATT GGCTCGTGTC CATCGGAATG CCTATGTATT CTGCACCTCT ACTGGCTGCT 
GGCTTTGACA CGTTGAGTCG TGTGTCGTCT TTAAATGAGG CGCAAGCGAG GGACGCGGGC 
CTGAAGGAGG AACATCACAT ACGGATTCTT CTCAGTGAAG CTCAACTCGT CTCAGCCGCC 
AGCTCAGGAC AGTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324106.1
CDS	915..5432
Translation	MMTNGPNIVYEWLKTLQLCQYVEAFVDNGYDDLEVCKQIGDPDLDAIGVFIPHHRQRIHDAVQRLKDDDRETATGLYFTLEPMPPVSPVYTSRMAEQYESRLRGSRSWTEQSSDRAARGVGYMGASRNLTLGNRKELEIYPKLKLKIMIRDKLIRDGINLARQPYSNKDGSLGNIDDLAQEYSEYYNTYFSDVSDRMEELRKRQVSQELDMEKSDTSLNSSQLRSDIQDSFGFSSEVSTPEAERKISLHKSSSEDGSGKWDNKKKNKSFWQNFRKSQKGVTRQASKGEDIGYVASEITMSDEERIQLMMMVKEKMITVEEALSRLKEYERNRQLSSSTDTAEWTDGSSPTVNLSSTCNSVEQSDDEQEDSVKFKRLHKLVNSTRRVRKKLIKVDDSKRHGSQDSLSLDAAPICDDINALYAEIHKKPAKCTDSSLSSLAQEQLSLDGDTDSLNTSPSTSSLDAWKPAHKLVKAPSTHPHGLIRPARKSSAGSGLGVAGGSGSSFSELDGLGDDNAKVSRSVTDGEMRKALSSLSHGVSTDSVYAFYGLTRPRPKPHPLLVSLDDINNTKRPPVSMWQRNKPDPNYAYSTKHLLYQRSCNAPKTAPSSTLPASPPTRELPLAKEKGKSCGSGVVGSWLFPPRRLKGRTAVSELNITYVVERSLYGHLNWTGLVRPITLSKADRRWLLEDERDANRKWASSMDRCTKRVLLRIQQKSRTCSFGGFDLSNRSLHVVNTAEPSEQEALYRDGVKSPTTSRISLGKKVKSVKETMRKRMSKKYVGSLSEQSSPDGTPSCPQSPQPDTDSLEKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSASNRESVRSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKRGDVIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLAEEEEKPKRAVRRRKKGRPPKPSSVEELLERINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIKDPQHRAVLMTAVELLQDYDSSSDPERSGLSGSQEKLLCEGHGLMADSPRDSGCYESNENLENGKSRKASRHSRGSSGFDCGDVKSPDYPTLPMTRSTEALQQQAKKERKFSKTFLPRPMKGFNLRNLGLKKGSAAAQTSRISLSRSCEELDSPQETPDSWKRSHSLGDIHWEQAFDQRKDQQGSEDRGRLESSTKEMKADLVDSKQDEKTNMAKRQTGSLQRPAVPTQLPVSPVSSLPASQSLFRQPVSAPTSPAPRTHPKKPPIPPPVPAKKSKERLPNGLRHPPLVLSGSVPNTPTLPPKPIYSPTTPSPSEGTPSTPASPGESAAPLSPPWLSDLPESACPQIHGAKIALSRKISHAKMLDLETLLEDRMHSEGIDLKEEPYSDKHGRCGIPQLLVQRYSEDFQQPAKDVASSLDQIRVKQLRKEHRMAIPSGGLTDMCRKPVSLVHVSSVSDWLVSIGMPMYSAPLLAAGFDTLSRVSSLNEAQARDAGLKEEHHIRILLSEAQLVSAASSGQS

Target ORF information:

RefSeq Version	XM_021468431.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SAM and SH3 domain containing 1a (sash1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468431.1

ORF Insert Sequence:

ATGATGACTA ATGGACCCAA CATCGTCTAC GAATGGCTAA AAACCCTTCA ACTTTGCCAA 
TATGTCGAGG CGTTTGTGGA TAACGGATAC GACGACTTGG AAGTTTGCAA ACAAATCGGA 
GACCCTGACC TGGATGCCAT CGGGGTTTTC ATTCCGCACC ACCGCCAGCG GATTCATGAT 
GCGGTGCAAA GGTTAAAAGA TGACGATAGA GAGACGGCTA CCGGGCTTTA TTTCACCCTA 
GAGCCGATGC CCCCGGTCTC ACCTGTATAC ACGAGTCGCA TGGCAGAGCA ATATGAGTCA 
AGGCTGCGGG GCTCCAGGTC ATGGACAGAG CAGAGCAGTG ATAGAGCCGC ACGCGGCGTG 
GGGTACATGG GGGCGTCCAG GAATCTGACC CTGGGGAACC GCAAGGAGCT GGAAATCTAC 
CCCAAACTGA AGCTGAAGAT CATGATCAGG GATAAGCTCA TTAGGGACGG GATAAACCTT 
GCCAGGCAGC CATACTCTAA TAAGGATGGC TCTCTGGGTA ATATAGATGA CCTGGCACAA 
GAGTACTCGG AGTACTACAA CACATACTTC AGTGACGTCA GCGATCGGAT GGAGGAACTG 
CGCAAACGGC AAGTCTCTCA AGAGCTAGAC ATGGAAAAAT CAGACACTAG TCTAAACTCT 
TCACAGCTCC GCTCAGACAT CCAGGATTCA TTTGGATTCA GCAGTGAGGT GTCCACACCA 
GAGGCTGAGA GAAAAATTTC TCTGCACAAA TCCAGCTCTG AGGATGGATC AGGAAAATGG 
GACAACAAGA AGAAAAACAA ATCCTTCTGG CAGAACTTCC GAAAGTCACA GAAGGGGGTC 
ACAAGGCAAG CGTCAAAAGG AGAAGACATT GGCTATGTGG CCAGTGAGAT CACAATGAGT 
GATGAAGAGC GAATCCAGCT TATGATGATG GTGAAAGAGA AGATGATCAC TGTAGAGGAA 
GCTCTGTCAC GGTTAAAGGA ATATGAGAGG AACAGGCAGT TGAGCAGCAG TACAGACACA 
GCAGAGTGGA CTGATGGATC CAGTCCCACT GTTAACCTCT CCTCCACCTG CAATTCTGTG 
GAGCAGTCAG ATGATGAGCA GGAGGACTCT GTGAAATTCA AAAGGCTTCA CAAGCTGGTG 
AACTCCACTC GACGTGTCCG CAAGAAACTC ATCAAAGTGG ATGATAGCAA GAGACATGGC 
TCACAAGATT CACTCAGTCT GGATGCAGCT CCCATATGCG ACGATATAAA TGCACTATAT 
GCAGAAATCC ACAAGAAGCC GGCAAAGTGT ACAGACTCCT CTTTGTCCTC ACTTGCCCAA 
GAGCAGCTTT CTCTGGACGG GGACACGGAC AGCCTGAACA CCTCGCCCTC CACCAGCAGC 
CTTGATGCCT GGAAACCTGC TCACAAATTA GTCAAGGCTC CCAGCACACA TCCCCATGGC 
CTGATCCGGC CAGCACGCAA GAGCAGTGCT GGGTCTGGGC TGGGTGTAGC GGGGGGCAGT 
GGCTCCTCGT TTTCTGAATT GGACGGTTTG GGAGACGACA ATGCTAAAGT CTCCCGCTCG 
GTTACAGACG GAGAGATGCG GAAGGCCTTG TCATCTCTAT CTCATGGGGT AAGTACTGAT 
AGCGTTTACG CTTTTTATGG CCTCACTAGG CCCCGCCCAA AGCCACACCC CCTCCTGGTG 
TCTTTAGATG ACATCAACAA CACCAAGCGG CCACCTGTTT CCATGTGGCA GCGAAATAAG 
CCTGACCCGA ACTATGCATA CTCTACCAAA CACCTGCTTT ACCAACGTTC CTGCAACGCT 
CCGAAAACCG CACCCTCCTC CACCCTACCT GCGTCTCCTC CCACTCGCGA GCTTCCGCTA 
GCCAAGGAGA AGGGGAAGAG TTGTGGGAGC GGGGTAGTGG GCAGCTGGCT GTTTCCACCA 
AGGCGTCTCA AAGGAAGGAC GGCAGTTAGT GAGCTCAACA TCACATATGT GGTGGAACGC 
AGCCTGTACG GACATTTGAA CTGGACTGGG CTGGTCAGGC CAATCACATT GAGCAAAGCG 
GACAGACGTT GGCTCCTGGA GGATGAAAGA GATGCCAACA GGAAATGGGC ATCTAGCATG 
GACCGCTGCA CCAAGCGTGT GCTCTTGCGC ATCCAACAAA AGTCTAGAAC ATGTAGCTTT 
GGAGGATTTG ACCTATCTAA TCGTTCCCTC CATGTGGTCA ACACAGCAGA GCCTAGCGAG 
CAGGAGGCTC TGTACAGGGA TGGCGTGAAA TCTCCCACCA CATCCCGTAT CTCTCTAGGA 
AAGAAAGTCA AGTCTGTCAA AGAGACTATG AGGAAACGCA TGTCCAAGAA ATATGTCGGG 
TCTCTCTCTG AACAGTCCAG TCCAGATGGG ACTCCCAGTT GTCCTCAGTC TCCTCAACCA 
GACACAGATT CTCTAGAGAA GCCCAAGCTC AAGGCAGGAG GGTCTGTGGA GAGTCTGCGC 
AGCTCTCTCA GTGGCCAAAG CTCCATGAGT GGGCAGACGG TGAGCACCAC AGACTCATCA 
GCCAGTAACA GAGAGAGTGT GAGGAGTGAG GATGGTGATG ATGAAGAGCC TCCATACAGA 
GGCCCGTTCT GTGGCAGAGC AAGAGTTCAC ACTGACTTCA CACCCAGTCC ATATGATACA 
GATTCTCTCA AGCTCAAGAG AGGAGATGTG ATTGACATCA TCAGTAAGCC GCCCATGGGC 
ACATGGATGG GTCTGCTCAA TAACAAAGTG GGCACGTTTA AGTTCATCTA TGTGGATGTT 
CTGGCAGAAG AAGAGGAGAA GCCCAAACGA GCTGTTCGCA GGAGGAAGAA GGGCCGACCA 
CCCAAACCCA GCTCAGTCGA GGAGCTTCTG GAGAGAATCA ACCTGAAGGA GCACATGCCA 
ACGTTTCTGT TTAACGGGTA TGAAGATCTG GACACGTTTA AGTTACTAGA GGAAGAAGAT 
CTGGATGAGC TCAACATCAA AGACCCACAA CACCGAGCCG TACTGATGAC AGCTGTTGAA 
CTGCTTCAGG ATTATGACAG CAGCAGTGAT CCAGAGCGCA GCGGTCTCTC TGGCTCTCAG 
GAGAAGCTCC TCTGTGAGGG CCATGGCCTC ATGGCTGACT CTCCACGGGA CTCTGGTTGC 
TACGAAAGCA ATGAAAACCT AGAGAATGGT AAGAGCCGAA AGGCATCGCG CCACAGCCGT 
GGTTCATCAG GGTTTGACTG TGGGGATGTA AAGTCTCCAG ATTATCCCAC TCTTCCCATG 
ACTCGCTCCA CTGAAGCTCT TCAACAGCAG GCCAAGAAGG AGCGCAAGTT CTCGAAGACC 
TTTCTGCCCC GACCCATGAA GGGCTTCAAT CTGCGAAACC TCGGCCTCAA GAAAGGCTCC 
GCAGCAGCTC AAACCTCCCG GATTTCGCTC AGCAGAAGCT GTGAGGAGTT GGACAGCCCC 
CAGGAGACCC CTGACTCTTG GAAGCGCTCT CATTCTCTGG GGGATATCCA CTGGGAACAG 
GCCTTCGATC AGAGGAAGGA CCAGCAGGGC AGTGAGGATA GAGGCAGGCT GGAGAGCAGC 
ACAAAAGAAA TGAAAGCAGA TCTTGTAGAC TCCAAACAGG ATGAGAAGAC CAATATGGCT 
AAAAGGCAGA CTGGCTCCTT GCAGAGGCCT GCAGTTCCCA CACAGCTTCC TGTGAGTCCG 
GTCAGTTCAT TGCCTGCTTC TCAGAGCTTA TTCCGTCAAC CTGTCTCTGC TCCGACAAGC 
CCAGCTCCAA GGACTCACCC TAAAAAGCCA CCCATCCCAC CTCCAGTGCC TGCTAAAAAG 
TCCAAAGAAC GTTTGCCGAA TGGTCTGCGA CACCCTCCGC TGGTGCTGTC CGGTTCAGTG 
CCAAACACTC CAACCCTACC CCCAAAGCCT ATATATTCTC CGACCACCCC CTCTCCCTCT 
GAGGGTACCC CATCAACCCC AGCATCACCA GGTGAATCTG CAGCACCTCT GAGCCCACCG 
TGGCTGTCTG ACCTGCCTGA ATCAGCATGT CCTCAAATCC ATGGAGCTAA AATCGCTCTC 
AGCAGGAAGA TCTCCCATGC CAAGATGCTT GACCTAGAGA CTCTGCTGGA GGACAGGATG 
CACTCGGAAG GGATCGACCT CAAAGAGGAG CCTTACTCTG ATAAACATGG CCGGTGTGGT 
ATTCCTCAGC TCTTAGTTCA GAGGTACTCA GAGGACTTCC AGCAGCCAGC GAAGGACGTG 
GCGTCCAGTC TTGATCAAAT CAGAGTTAAA CAACTCCGCA AGGAGCACCG CATGGCTATT 
CCCTCAGGAG GTTTGACTGA CATGTGTCGG AAGCCAGTGT CTCTGGTTCA TGTGAGCTCT 
GTCTCTGATT GGCTCGTGTC CATCGGAATG CCTATGTATT CTGCACCTCT ACTGGCTGCT 
GGCTTTGACA CGTTGAGTCG TGTGTCGTCT TTAAATGAGG CGCAAGCGAG GGACGCGGGC 
CTGAAGGAGG AACATCACAT ACGGATTCTT CTCAGTGAAG CTCAACTCGT CTCAGCCGCC 
AGCTCAGGAC AGTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Rab32 and Rab38 genes in chordate pigmentation: an evolutionary perspective.
BMC evolutionary biology1626(2016 Jan)
Coppola U,Annona G,D'Aniello S,Ristoratore F

Fmrp Interacts with Adar and Regulates RNA Editing, Synaptic Density and Locomotor Activity in Zebrafish.
PLoS genetics11(12)e1005702(2015 Dec)
Shamay-Ramot A,Khermesh K,Porath HT,Barak M,Pinto Y,Wachtel C,Zilberberg A,Lerer-Goldshtein T,Efroni S,Levanon EY,Appelbaum L

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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sash1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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