SASH1 cDNA ORF clone, Bos taurus(cattle)

The following SASH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SASH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa238104 XM_002690315.5
Latest version!
Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa238105 XM_024997050.1
Latest version!
Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa91514 XM_015464850.1
Latest version!
Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa91514 XM_015472900.1
Latest version!
Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa06173 XM_005211030.4
Latest version!
Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa06173 XM_005199430.3
Latest version!
Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa06174 XM_005211031.4
Latest version!
Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa06174 XM_005199431.3
Latest version!
Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa06172 XM_002690315.4
Latest version!
Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa06172 XM_588302.7
Latest version!
Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
OBa06173 XM_005211030.3 Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa06174 XM_005211031.3 Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa238104
    Clone ID Related Accession (Same CDS sequence) XM_002690315.5
    Accession Version XM_002690315.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product SAM and SH3 domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002690315.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGCAAG ACGCGGGAGC CGCCGGCGCG GCGCCCGAGC CGGAACCCGA GCCCGCTTCG 
    GAGCCCGAGG TGGGCGCGGG CATGTCCGAG GCGTTCTCCC GGCTCTGGAC CGACGTGATG
    GGCATCCTGG ATGGCTCCCT GGGAAACATC GATGACCTGG CTCAGCAGTA TGCAGATTAC
    TACAATACCT GCTTCTCCGA CGTGTGTGAG AGGATGGAGG AGCTGCGGAA ACGGCGGGTT
    TCCCAGGACC TGGACGTGGA GAAACCCGAA GCCAGCCCCA CGTCACTTCA GCTGCGGTCT
    CAGATCGAAG AGTCGCTTGG CTTCTGTAGC GCTGTATCAA CACCAGAAGT GGAAAGAAAG
    AATCCTCTCC ATAAGTCAAA CTCGGAAGAC AGCTCTGTAG GAAAGGGAGA TTGGAAGAAG
    AAAAATAAGT ATTTCTGGCA GAACTTCCGA AAGAATCAGA AAGGAATAAT GAGACAGACT
    TCAAAAGGAG AAGATGTGGG TTACGTTGCA AGCGAGATAA CAATGAGTGA TGAGGAACGG
    ATTCAGTTGA TGATGATGGT CAAGGAAAAG ATGATCACCA TCGAAGAAGC GCTTGCTCGG
    CTCAAGGAGT ATGAGGCCCA GCACCGGCAG TCCACTGCCT TGGATCCTGC CGACTGGCCG
    GACGGTTCTT ACCCAACATT TGAAGGCTCA TCAAACTGCA ATTCAAGAGA ACAGTCGGAG
    GATGAGACGG AGGAGTCGGT GAAGTTTAAG AGGTTACACA AGCTGGTAAA CTCCACTCGC
    AGAGTGAGAA AGAAACTCAT CAGGGTGGAA GAAATGAAAA AGCCCAGCAC AGAAGGCGGG
    GAGGAACAGG TGTTTGAGAA TTCCCCGGTC CCAGATGAAA GGTCCGCCCT GTACTCCGGC
    GTGCACAAGA AGCCCTTTTT CTTTGATGCT TCTCCTGAGA AACCCCCAGA AGATGACTCA
    GACTCTCTCA CCACTGCTCC ATCGTCCAGT AGCCTGGACA CCTGGGGTGC CGGCCGCAAA
    TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC CGGGGCCTGA TCAAGCCCCC CAAGAAGATG
    GGGACGTTCT TCTCGTACCC GGAAGACGAC AAGGCCCAGA AGGTGTCCCG CTCCCTCACC
    GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC CTGAGCCACG GGAGAACCTG CAGTTTTGGA
    GGATTTGACT TGACAAATCG TTCTCTGCAC ATTGGCAGTA ATAATTCGGA CCCAATGGGT
    AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA ATCAAATCAC CCACTGCCTC CCGAATCTCT
    CTTGGAAAAA AGGTGAAATC CGTGAAAGAG ACAATGAGGA AGAGAATGTC TAAAAAATAT
    AGCAGCTCTG TCTCTGAGCA GGATTCAGGC CTTGATGGGA TGCCGGGCTC CCCTCCGTCC
    TCTCAGCCTG ACAGTGAACA CATGGACAAG CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA
    AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC TCGATGAGTG GTCAGACAGT GAGCACCACC
    GATTCCTCGA CCAGCAATAG GGAGAGTGTC AAGTCGGAAG ATGGTGATGA CGAGGAGCCC
    CCGTACCGGG GCCCGTTCTG TGGGCGTGCC AGGGTGCACA CCGACTTCAC GCCCAGCCCC
    TACGACACAG ACTCCCTCAA GCTCAAGAAA GGAGACATCA TTGATATAAT CAGCAAACCC
    CCCATGGGCA CCTGGATGGG CCTGCTGAAC AACAAGGTGG GCACGTTCAA ATTCATCTAC
    GTGGATGTGC TCAACGAGGA AGAGGAGAAG CCCAAGCGCG CCACCAGGAG GAGGAGGAAA
    GGGAGACCCC CTCAGCCGAA GTCAGTGGAG GACCTCCTGG ATCGGATCAA CCTCAAAGAG
    CACATGCCCA CTTTCCTGTT CAATGGCTAT GAAGATCTGG ACACCTTTAA GCTCCTAGAG
    GAGGAGGACT TGGACGAGTT AAATATCAGG GACCCAGAGC ACAGAGCTGT TCTCTTGACA
    GCAGTGGAGT TGTTACAGGA ATATGACAGT AACAGCGACC AGTCCGGATC CCAGGAGAAG
    CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA TGCTCTCCAC GAGACTCAGG ATGCTATGAA
    AGCAGTGAGA ATTTGGAAAA CGGCAAGACT CGGAAAACCG GCCTCTCTGC CAAGTCATCA
    ACCGAGTCCA GCTTGAAGTC TTTCAACAGG AACCAGCTGG GCAACTACCC AACCTTGCCT
    TTGACGAAGT CAGTGGACGC TTTGAAGCAG GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC
    ACCCCTCTCG CCTCCAAGAG CTGTGATCCC CCGTGTGTGA CTGATCTGAA TAAAAACCGA
    AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC TGTGAGACCC TAGAGGGCCC ACAGACCGTG
    GAGACTTGGC CCCGGTCCCA CTCCCTGGAC AACCTTCAAG GAGAGCCTGA GAAAGATGTG
    CCTGGCGAGG TGACAGAACC CTCTCCTGAG ATTGTACCTG AAGTGCCACA AAAGACAGCC
    CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA CGAGATTCTG CTGTTGACAA TGCACTGCTA
    CTGACCCAAA GCAAGAGATT TTCTGAACCT CAGAAAACGA CTGCTAAGAA ACTGGATGGC
    CCAATGGGGC CCTTTTCACG GGGTGCATCC CCCCCTCCAT GTTTGCCCAA AACCTTTGAC
    ACCCAGCCTC CTGCCATTAA ACCCAGTTTA ACACGGACGC CTCTGGAGGG TCATAGGAAA
    GGACTCGATT TCGAAGGAAC ACATCACCCC CCGGGCACCA AAGAAGGCAT GGACGCTGAG
    CAGAGGGGCC CCGAGGTCAG AACGCAGGCC AAACCTCCTG TCCAGCCTCC GCCCGTTCCC
    GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC GGGCTGCATC CTGTCCCTCC TGGTCCCCCC
    GGCGTCCCCA GCCCCGACGC ACCCGGCCTG CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC
    AGTGACTGTC CCCTGGTGGT GGCGGTGGCC AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC
    CCACCAAGCA CCAGGCCACC ACCCTGGCTG TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG
    GAGCACGGCG TGCGGCTCGG GCCCGCGCTG GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG
    GACCTAGAAA TGCTCACTGA GAACAAGCTG CGGGCGGAAG GCATTGACCT CACCGAGGAG
    CCCTATTCTG ATAAGCACGG CCGCTGTGGA ATTCCTGAGG CCCTGGTGCA GAGATATGCA
    GAAGACTTAG ACCAGCCGGA AAGGGATGTC GCCACCAACA TGGACCAGAT CCGTGTCAAG
    CTGCTTCGGA AGCAGCACCG CATGGCGATC CCAAGCGGTG GACTCACAGA AATCTGCAGG
    AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC GTGTCAGATT GGCTGGTTTC CATCGGTCTG
    CCCATGTATG CCAGCGCCCT CTCTGAGGCG GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT
    CTGTCCCACA CTTGCCTTCA GGAGGCTGGC ATCACAGAGG AGAGACACAT AAGCAAGCTC
    GTGTCTGCAG CCAGACTCTT CAAACTGCCA CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002690361.2
    CDS61..3771
    Translation

    Target ORF information:

    RefSeq Version XM_002690315.5
    Organism Bos taurus(cattle)
    Definition Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002690315.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGCAAG ACGCGGGAGC CGCCGGCGCG GCGCCCGAGC CGGAACCCGA GCCCGCTTCG 
    GAGCCCGAGG TGGGCGCGGG CATGTCCGAG GCGTTCTCCC GGCTCTGGAC CGACGTGATG
    GGCATCCTGG ATGGCTCCCT GGGAAACATC GATGACCTGG CTCAGCAGTA TGCAGATTAC
    TACAATACCT GCTTCTCCGA CGTGTGTGAG AGGATGGAGG AGCTGCGGAA ACGGCGGGTT
    TCCCAGGACC TGGACGTGGA GAAACCCGAA GCCAGCCCCA CGTCACTTCA GCTGCGGTCT
    CAGATCGAAG AGTCGCTTGG CTTCTGTAGC GCTGTATCAA CACCAGAAGT GGAAAGAAAG
    AATCCTCTCC ATAAGTCAAA CTCGGAAGAC AGCTCTGTAG GAAAGGGAGA TTGGAAGAAG
    AAAAATAAGT ATTTCTGGCA GAACTTCCGA AAGAATCAGA AAGGAATAAT GAGACAGACT
    TCAAAAGGAG AAGATGTGGG TTACGTTGCA AGCGAGATAA CAATGAGTGA TGAGGAACGG
    ATTCAGTTGA TGATGATGGT CAAGGAAAAG ATGATCACCA TCGAAGAAGC GCTTGCTCGG
    CTCAAGGAGT ATGAGGCCCA GCACCGGCAG TCCACTGCCT TGGATCCTGC CGACTGGCCG
    GACGGTTCTT ACCCAACATT TGAAGGCTCA TCAAACTGCA ATTCAAGAGA ACAGTCGGAG
    GATGAGACGG AGGAGTCGGT GAAGTTTAAG AGGTTACACA AGCTGGTAAA CTCCACTCGC
    AGAGTGAGAA AGAAACTCAT CAGGGTGGAA GAAATGAAAA AGCCCAGCAC AGAAGGCGGG
    GAGGAACAGG TGTTTGAGAA TTCCCCGGTC CCAGATGAAA GGTCCGCCCT GTACTCCGGC
    GTGCACAAGA AGCCCTTTTT CTTTGATGCT TCTCCTGAGA AACCCCCAGA AGATGACTCA
    GACTCTCTCA CCACTGCTCC ATCGTCCAGT AGCCTGGACA CCTGGGGTGC CGGCCGCAAA
    TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC CGGGGCCTGA TCAAGCCCCC CAAGAAGATG
    GGGACGTTCT TCTCGTACCC GGAAGACGAC AAGGCCCAGA AGGTGTCCCG CTCCCTCACC
    GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC CTGAGCCACG GGAGAACCTG CAGTTTTGGA
    GGATTTGACT TGACAAATCG TTCTCTGCAC ATTGGCAGTA ATAATTCGGA CCCAATGGGT
    AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA ATCAAATCAC CCACTGCCTC CCGAATCTCT
    CTTGGAAAAA AGGTGAAATC CGTGAAAGAG ACAATGAGGA AGAGAATGTC TAAAAAATAT
    AGCAGCTCTG TCTCTGAGCA GGATTCAGGC CTTGATGGGA TGCCGGGCTC CCCTCCGTCC
    TCTCAGCCTG ACAGTGAACA CATGGACAAG CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA
    AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC TCGATGAGTG GTCAGACAGT GAGCACCACC
    GATTCCTCGA CCAGCAATAG GGAGAGTGTC AAGTCGGAAG ATGGTGATGA CGAGGAGCCC
    CCGTACCGGG GCCCGTTCTG TGGGCGTGCC AGGGTGCACA CCGACTTCAC GCCCAGCCCC
    TACGACACAG ACTCCCTCAA GCTCAAGAAA GGAGACATCA TTGATATAAT CAGCAAACCC
    CCCATGGGCA CCTGGATGGG CCTGCTGAAC AACAAGGTGG GCACGTTCAA ATTCATCTAC
    GTGGATGTGC TCAACGAGGA AGAGGAGAAG CCCAAGCGCG CCACCAGGAG GAGGAGGAAA
    GGGAGACCCC CTCAGCCGAA GTCAGTGGAG GACCTCCTGG ATCGGATCAA CCTCAAAGAG
    CACATGCCCA CTTTCCTGTT CAATGGCTAT GAAGATCTGG ACACCTTTAA GCTCCTAGAG
    GAGGAGGACT TGGACGAGTT AAATATCAGG GACCCAGAGC ACAGAGCTGT TCTCTTGACA
    GCAGTGGAGT TGTTACAGGA ATATGACAGT AACAGCGACC AGTCCGGATC CCAGGAGAAG
    CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA TGCTCTCCAC GAGACTCAGG ATGCTATGAA
    AGCAGTGAGA ATTTGGAAAA CGGCAAGACT CGGAAAACCG GCCTCTCTGC CAAGTCATCA
    ACCGAGTCCA GCTTGAAGTC TTTCAACAGG AACCAGCTGG GCAACTACCC AACCTTGCCT
    TTGACGAAGT CAGTGGACGC TTTGAAGCAG GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC
    ACCCCTCTCG CCTCCAAGAG CTGTGATCCC CCGTGTGTGA CTGATCTGAA TAAAAACCGA
    AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC TGTGAGACCC TAGAGGGCCC ACAGACCGTG
    GAGACTTGGC CCCGGTCCCA CTCCCTGGAC AACCTTCAAG GAGAGCCTGA GAAAGATGTG
    CCTGGCGAGG TGACAGAACC CTCTCCTGAG ATTGTACCTG AAGTGCCACA AAAGACAGCC
    CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA CGAGATTCTG CTGTTGACAA TGCACTGCTA
    CTGACCCAAA GCAAGAGATT TTCTGAACCT CAGAAAACGA CTGCTAAGAA ACTGGATGGC
    CCAATGGGGC CCTTTTCACG GGGTGCATCC CCCCCTCCAT GTTTGCCCAA AACCTTTGAC
    ACCCAGCCTC CTGCCATTAA ACCCAGTTTA ACACGGACGC CTCTGGAGGG TCATAGGAAA
    GGACTCGATT TCGAAGGAAC ACATCACCCC CCGGGCACCA AAGAAGGCAT GGACGCTGAG
    CAGAGGGGCC CCGAGGTCAG AACGCAGGCC AAACCTCCTG TCCAGCCTCC GCCCGTTCCC
    GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC GGGCTGCATC CTGTCCCTCC TGGTCCCCCC
    GGCGTCCCCA GCCCCGACGC ACCCGGCCTG CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC
    AGTGACTGTC CCCTGGTGGT GGCGGTGGCC AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC
    CCACCAAGCA CCAGGCCACC ACCCTGGCTG TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG
    GAGCACGGCG TGCGGCTCGG GCCCGCGCTG GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG
    GACCTAGAAA TGCTCACTGA GAACAAGCTG CGGGCGGAAG GCATTGACCT CACCGAGGAG
    CCCTATTCTG ATAAGCACGG CCGCTGTGGA ATTCCTGAGG CCCTGGTGCA GAGATATGCA
    GAAGACTTAG ACCAGCCGGA AAGGGATGTC GCCACCAACA TGGACCAGAT CCGTGTCAAG
    CTGCTTCGGA AGCAGCACCG CATGGCGATC CCAAGCGGTG GACTCACAGA AATCTGCAGG
    AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC GTGTCAGATT GGCTGGTTTC CATCGGTCTG
    CCCATGTATG CCAGCGCCCT CTCTGAGGCG GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT
    CTGTCCCACA CTTGCCTTCA GGAGGCTGGC ATCACAGAGG AGAGACACAT AAGCAAGCTC
    GTGTCTGCAG CCAGACTCTT CAAACTGCCA CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238105
    Clone ID Related Accession (Same CDS sequence) XM_024997050.1
    Accession Version XM_024997050.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3441bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product SAM and SH3 domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGATTA CTACAATACC TGCTTCTCCG ACGTGTGTGA GAGGATGGAG GAGCTGCGGA 
    AACGGCGGGT TTCCCAGGAC CTGGACGTGG AATCCTCTCC ATAAGTCAAA CTCGGAAGAC
    AGCTCTGTAG GAAAGGGAGA TTGGAAGAAG AAAAATAAGT ATTTCTGGCA GAACTTCCGA
    AAGAATCAGA AAGGAATAAT GAGACAGACT TCAAAAGGAG AAGATGTGGG TTACGTTGCA
    AGCGAGATAA CAATGAGTGA TGAGGAACGG ATTCAGTTGA TGATGATGGT CAAGGAAAAG
    ATGATCACCA TCGAAGAAGC GCTTGCTCGG CTCAAGGAGT ATGAGGCCCA GCACCGGCAG
    TCCACTGCCT TGGATCCTGC CGACTGGCCG GACGGTTCTT ACCCAACATT TGAAGGCTCA
    TCAAACTGCA ATTCAAGAGA ACAGTCGGAG GATGAGACGG AGGAGTCGGT GAAGTTTAAG
    AGGTTACACA AGCTGGTAAA CTCCACTCGC AGAGTGAGAA AGAAACTCAT CAGGGTGGAA
    GAAATGAAAA AGCCCAGCAC AGAAGGCGGG GAGGAACAGG TGTTTGAGAA TTCCCCGGTC
    CCAGATGAAA GGTCCGCCCT GTACTCCGGC GTGCACAAGA AGCCCTTTTT CTTTGATGCT
    TCTCCTGAGA AACCCCCAGA AGATGACTCA GACTCTCTCA CCACTGCTCC ATCGTCCAGT
    AGCCTGGACA CCTGGGGTGC CGGCCGCAAA TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC
    CGGGGCCTGA TCAAGCCCCC CAAGAAGATG GGGACGTTCT TCTCGTACCC GGAAGACGAC
    AAGGCCCAGA AGGTGTCCCG CTCCCTCACC GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC
    CTGAGCCACG GGAGAACCTG CAGTTTTGGA GGATTTGACT TGACAAATCG TTCTCTGCAC
    ATTGGCAGTA ATAATTCGGA CCCAATGGGT AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA
    ATCAAATCAC CCACTGCCTC CCGAATCTCT CTTGGAAAAA AGGTGAAATC CGTGAAAGAG
    ACAATGAGGA AGAGAATGTC TAAAAAATAT AGCAGCTCTG TCTCTGAGCA GGATTCAGGC
    CTTGATGGGA TGCCGGGCTC CCCTCCGTCC TCTCAGCCTG ACAGTGAACA CATGGACAAG
    CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC
    TCGATGAGTG GTCAGACAGT GAGCACCACC GATTCCTCGA CCAGCAATAG GGAGAGTGTC
    AAGTCGGAAG ATGGTGATGA CGAGGAGCCC CCGTACCGGG GCCCGTTCTG TGGGCGTGCC
    AGGGTGCACA CCGACTTCAC GCCCAGCCCC TACGACACAG ACTCCCTCAA GCTCAAGAAA
    GGAGACATCA TTGATATAAT CAGCAAACCC CCCATGGGCA CCTGGATGGG CCTGCTGAAC
    AACAAGGTGG GCACGTTCAA ATTCATCTAC GTGGATGTGC TCAACGAGGA AGAGGAGAAG
    CCCAAGCGCG CCACCAGGAG GAGGAGGAAA GGGAGACCCC CTCAGCCGAA GTCAGTGGAG
    GACCTCCTGG ATCGGATCAA CCTCAAAGAG CACATGCCCA CTTTCCTGTT CAATGGCTAT
    GAAGATCTGG ACACCTTTAA GCTCCTAGAG GAGGAGGACT TGGACGAGTT AAATATCAGG
    GACCCAGAGC ACAGAGCTGT TCTCTTGACA GCAGTGGAGT TGTTACAGGA ATATGACAGT
    AACAGCGACC AGTCCGGATC CCAGGAGAAG CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA
    TGCTCTCCAC GAGACTCAGG ATGCTATGAA AGCAGTGAGA ATTTGGAAAA CGGCAAGACT
    CGGAAAACCG GCCTCTCTGC CAAGTCATCA ACCGAGTCCA GCTTGAAGTC TTTCAACAGG
    AACCAGCTGG GCAACTACCC AACCTTGCCT TTGACGAAGT CAGTGGACGC TTTGAAGCAG
    GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC ACCCCTCTCG CCTCCAAGAG CTGTGATCCC
    CCGTGTGTGA CTGATCTGAA TAAAAACCGA AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC
    TGTGAGACCC TAGAGGGCCC ACAGACCGTG GAGACTTGGC CCCGGTCCCA CTCCCTGGAC
    AACCTTCAAG GAGAGCCTGA GAAAGATGTG CCTGGCGAGG TGACAGAACC CTCTCCTGAG
    ATTGTACCTG AAGTGCCACA AAAGACAGCC CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA
    CGAGATTCTG CTGTTGACAA TGCACTGCTA CTGACCCAAA GCAAGAGATT TTCTGAACCT
    CAGAAAACGA CTGCTAAGAA ACTGGATGGC CCAATGGGGC CCTTTTCACG GGGTGCATCC
    CCCCCTCCAT GTTTGCCCAA AACCTTTGAC ACCCAGCCTC CTGCCATTAA ACCCAGTTTA
    ACACGGACGC CTCTGGAGGG TCATAGGAAA GGACTCGATT TCGAAGGAAC ACATCACCCC
    CCGGGCACCA AAGAAGGCAT GGACGCTGAG CAGAGGGGCC CCGAGGTCAG AACGCAGGCC
    AAACCTCCTG TCCAGCCTCC GCCCGTTCCC GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC
    GGGCTGCATC CTGTCCCTCC TGGTCCCCCC GGCGTCCCCA GCCCCGACGC ACCCGGCCTG
    CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC AGTGACTGTC CCCTGGTGGT GGCGGTGGCC
    AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC CCACCAAGCA CCAGGCCACC ACCCTGGCTG
    TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG GAGCACGGCG TGCGGCTCGG GCCCGCGCTG
    GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG GACCTAGAAA TGCTCACTGA GAACAAGCTG
    CGGGCGGAAG GCATTGACCT CACCGAGGAG CCCTATTCTG ATAAGCACGG CCGCTGTGGA
    ATTCCTGAGG CCCTGGTGCA GAGATATGCA GAAGACTTAG ACCAGCCGGA AAGGGATGTC
    GCCACCAACA TGGACCAGAT CCGTGTCAAG CTGCTTCGGA AGCAGCACCG CATGGCGATC
    CCAAGCGGTG GACTCACAGA AATCTGCAGG AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC
    GTGTCAGATT GGCTGGTTTC CATCGGTCTG CCCATGTATG CCAGCGCCCT CTCTGAGGCG
    GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT CTGTCCCACA CTTGCCTTCA GGAGGCTGGC
    ATCACAGAGG AGAGACACAT AAGCAAGCTC GTGTCTGCAG CCAGACTCTT CAAACTGCCA
    CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852818.1
    CDS5..3445
    Translation

    Target ORF information:

    RefSeq Version XM_024997050.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997050.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGATTA CTACAATACC TGCTTCTCCG ACGTGTGTGA GAGGATGGAG GAGCTGCGGA 
    AACGGCGGGT TTCCCAGGAC CTGGACGTGG AATCCTCTCC ATAAGTCAAA CTCGGAAGAC
    AGCTCTGTAG GAAAGGGAGA TTGGAAGAAG AAAAATAAGT ATTTCTGGCA GAACTTCCGA
    AAGAATCAGA AAGGAATAAT GAGACAGACT TCAAAAGGAG AAGATGTGGG TTACGTTGCA
    AGCGAGATAA CAATGAGTGA TGAGGAACGG ATTCAGTTGA TGATGATGGT CAAGGAAAAG
    ATGATCACCA TCGAAGAAGC GCTTGCTCGG CTCAAGGAGT ATGAGGCCCA GCACCGGCAG
    TCCACTGCCT TGGATCCTGC CGACTGGCCG GACGGTTCTT ACCCAACATT TGAAGGCTCA
    TCAAACTGCA ATTCAAGAGA ACAGTCGGAG GATGAGACGG AGGAGTCGGT GAAGTTTAAG
    AGGTTACACA AGCTGGTAAA CTCCACTCGC AGAGTGAGAA AGAAACTCAT CAGGGTGGAA
    GAAATGAAAA AGCCCAGCAC AGAAGGCGGG GAGGAACAGG TGTTTGAGAA TTCCCCGGTC
    CCAGATGAAA GGTCCGCCCT GTACTCCGGC GTGCACAAGA AGCCCTTTTT CTTTGATGCT
    TCTCCTGAGA AACCCCCAGA AGATGACTCA GACTCTCTCA CCACTGCTCC ATCGTCCAGT
    AGCCTGGACA CCTGGGGTGC CGGCCGCAAA TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC
    CGGGGCCTGA TCAAGCCCCC CAAGAAGATG GGGACGTTCT TCTCGTACCC GGAAGACGAC
    AAGGCCCAGA AGGTGTCCCG CTCCCTCACC GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC
    CTGAGCCACG GGAGAACCTG CAGTTTTGGA GGATTTGACT TGACAAATCG TTCTCTGCAC
    ATTGGCAGTA ATAATTCGGA CCCAATGGGT AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA
    ATCAAATCAC CCACTGCCTC CCGAATCTCT CTTGGAAAAA AGGTGAAATC CGTGAAAGAG
    ACAATGAGGA AGAGAATGTC TAAAAAATAT AGCAGCTCTG TCTCTGAGCA GGATTCAGGC
    CTTGATGGGA TGCCGGGCTC CCCTCCGTCC TCTCAGCCTG ACAGTGAACA CATGGACAAG
    CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC
    TCGATGAGTG GTCAGACAGT GAGCACCACC GATTCCTCGA CCAGCAATAG GGAGAGTGTC
    AAGTCGGAAG ATGGTGATGA CGAGGAGCCC CCGTACCGGG GCCCGTTCTG TGGGCGTGCC
    AGGGTGCACA CCGACTTCAC GCCCAGCCCC TACGACACAG ACTCCCTCAA GCTCAAGAAA
    GGAGACATCA TTGATATAAT CAGCAAACCC CCCATGGGCA CCTGGATGGG CCTGCTGAAC
    AACAAGGTGG GCACGTTCAA ATTCATCTAC GTGGATGTGC TCAACGAGGA AGAGGAGAAG
    CCCAAGCGCG CCACCAGGAG GAGGAGGAAA GGGAGACCCC CTCAGCCGAA GTCAGTGGAG
    GACCTCCTGG ATCGGATCAA CCTCAAAGAG CACATGCCCA CTTTCCTGTT CAATGGCTAT
    GAAGATCTGG ACACCTTTAA GCTCCTAGAG GAGGAGGACT TGGACGAGTT AAATATCAGG
    GACCCAGAGC ACAGAGCTGT TCTCTTGACA GCAGTGGAGT TGTTACAGGA ATATGACAGT
    AACAGCGACC AGTCCGGATC CCAGGAGAAG CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA
    TGCTCTCCAC GAGACTCAGG ATGCTATGAA AGCAGTGAGA ATTTGGAAAA CGGCAAGACT
    CGGAAAACCG GCCTCTCTGC CAAGTCATCA ACCGAGTCCA GCTTGAAGTC TTTCAACAGG
    AACCAGCTGG GCAACTACCC AACCTTGCCT TTGACGAAGT CAGTGGACGC TTTGAAGCAG
    GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC ACCCCTCTCG CCTCCAAGAG CTGTGATCCC
    CCGTGTGTGA CTGATCTGAA TAAAAACCGA AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC
    TGTGAGACCC TAGAGGGCCC ACAGACCGTG GAGACTTGGC CCCGGTCCCA CTCCCTGGAC
    AACCTTCAAG GAGAGCCTGA GAAAGATGTG CCTGGCGAGG TGACAGAACC CTCTCCTGAG
    ATTGTACCTG AAGTGCCACA AAAGACAGCC CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA
    CGAGATTCTG CTGTTGACAA TGCACTGCTA CTGACCCAAA GCAAGAGATT TTCTGAACCT
    CAGAAAACGA CTGCTAAGAA ACTGGATGGC CCAATGGGGC CCTTTTCACG GGGTGCATCC
    CCCCCTCCAT GTTTGCCCAA AACCTTTGAC ACCCAGCCTC CTGCCATTAA ACCCAGTTTA
    ACACGGACGC CTCTGGAGGG TCATAGGAAA GGACTCGATT TCGAAGGAAC ACATCACCCC
    CCGGGCACCA AAGAAGGCAT GGACGCTGAG CAGAGGGGCC CCGAGGTCAG AACGCAGGCC
    AAACCTCCTG TCCAGCCTCC GCCCGTTCCC GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC
    GGGCTGCATC CTGTCCCTCC TGGTCCCCCC GGCGTCCCCA GCCCCGACGC ACCCGGCCTG
    CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC AGTGACTGTC CCCTGGTGGT GGCGGTGGCC
    AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC CCACCAAGCA CCAGGCCACC ACCCTGGCTG
    TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG GAGCACGGCG TGCGGCTCGG GCCCGCGCTG
    GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG GACCTAGAAA TGCTCACTGA GAACAAGCTG
    CGGGCGGAAG GCATTGACCT CACCGAGGAG CCCTATTCTG ATAAGCACGG CCGCTGTGGA
    ATTCCTGAGG CCCTGGTGCA GAGATATGCA GAAGACTTAG ACCAGCCGGA AAGGGATGTC
    GCCACCAACA TGGACCAGAT CCGTGTCAAG CTGCTTCGGA AGCAGCACCG CATGGCGATC
    CCAAGCGGTG GACTCACAGA AATCTGCAGG AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC
    GTGTCAGATT GGCTGGTTTC CATCGGTCTG CCCATGTATG CCAGCGCCCT CTCTGAGGCG
    GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT CTGTCCCACA CTTGCCTTCA GGAGGCTGGC
    ATCACAGAGG AGAGACACAT AAGCAAGCTC GTGTCTGCAG CCAGACTCTT CAAACTGCCA
    CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91514
    Clone ID Related Accession (Same CDS sequence) XM_015464850.1 , XM_015472900.1
    Accession Version XM_015464850.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3603bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product SAM and SH3 domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645735.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGGATC AAGATGGCAG GGATGGCTCC CTGGGAAACA TCGATGACCT GGCTCAGCAG 
    TATGCAGATT ACTACAATAC CTGCTTCTCC GACGTGTGTG AGAGGATGGA GGAGCTGCGG
    AAACGGCGGG TTTCCCAGGA CCTGGACGTG GAGAAACCCG AAGCCAGCCC CACGTCACTT
    CAGCTGCGGT CTCAGATCGA AGAGTCGCTT GGCTTCTGTA GCGCTGTATC AACACCAGAA
    GTGGAAAGAA AGAATCCTCT CCATAAGTCA AACTCGGAAG ACAGCTCTGT AGGAAAGGGA
    GATTGGAAGA AGAAAAATAA GTATTTCTGG CAGAACTTCC GAAAGAATCA GAAAGGAATA
    ATGAGACAGA CTTCAAAAGG AGAAGATGTG GGTTACGTTG CAAGCGAGAT AACAATGAGT
    GATGAGGAAC GGATTCAGTT GATGATGATG GTCAAGGAAA AGATGATCAC CATCGAAGAA
    GCGCTTGCTC GGCTCAAGGA GTATGAGGCC CAGCACCGGC AGTCCACTGC CTTGGATCCT
    GCCAACTGGC CGGACGGTTC TTACCCAACA TTTGAAGGCT CATCAAACTG CAATTCAAGA
    GAACAGTCGG AGGATGAGAC GGAGGAGTCG GTGAAGTTTA AGAGGTTACA CAAGCTGGTA
    AACTCCACTC GCAGAGTGAG AAAGAAACTC ATCAGGGTGG AAGAAATGAA AAAGCCCAGC
    ACAGAAGGCG GGGAGGAACA GGTGTTTGAG AATTCCCCGG TCCCAGATGA AAGGTCCGCC
    CTGTACTCCG GCGTGCACAA GAAGCCCTTT TTCTTTGATG CTTCTCCTGA GAAACCCCCA
    GAAGATGACT CAGACTCTCT CACCACTGCT CCATCGTCCA GTAGCCTGGA CACCTGGGGT
    GCCGGCCGCA AATTGGTCAA AACCTTCAGC AAAGGAGAGA GCCGGGGCCT GATCAAGCCC
    CCCAAGAAGA TGGGGACGTT CTTCTCGTAC CCGGAAGACG ACAAGGCCCA GAAGGTGTCC
    CGCTCCCTCA CCGAAGGGGA GATGAAGAAG GGGCTTGGGT CCCTGAGCCA CGGGAGAACC
    TGCAGTTTTG GAGGATTTGA CTTGACAAAT CGTTCTCTGC ACATTGGCAG TAATAATTCG
    GACCCAATGG GTAAAGAAGG AGATTTTGTG TATAAAGAAG TAATCAAATC ACCCACTGCC
    TCCCGAATCT CTCTTGGAAA AAAGGTGAAA TCCGTGAAAG AGACAATGAG GAAGAGAATG
    TCTAAAAAAT ATAGCAGCTC TGTCTCTGAG CAGGATTCAG GCCTTGATGG GATGCCGGGC
    TCCCCTCCGT CCTCTCAGCC TGACAGTGAA CACATGGACA AGCCCAAGCT CAAAGCCGGA
    GGTTCTGTGG AAAGTCTGCG GAGTTCTCTG AGCGGTCAAA GCTCGATGAG TGGTCAGACA
    GTGAGCACCA CCGATTCCTC GACCAGCAAT AGGGAGAGTG TCAAGTCGGA AGATGGTGAT
    GACGAGGAGC CCCCGTACCG GGGCCCGTTC TGTGGGCGTG CCAGGGTGCA CACCGACTTC
    ACGCCCAGCC CCTACGACAC AGACTCCCTC AAGCTCAAGA AAGGAGACAT CATTGATATA
    ATCAGCAAAC CCCCCATGGG CACCTGGATG GGCCTGCTGA ACAACAAGGT GGGCACGTTC
    AAATTCATCT ACGTGGATGT GCTCAACGAG GAAGAGGAGA AGCCCAAGCG CGCCACCAGG
    AGGAGGAGGA AAGGGAGACC CCCTCAGCCG AAGTCAGTGG AGGACCTCCT GGATCGGATC
    AACCTCAAAG AGCACATGCC CACTTTCCTG TTCAATGGCT ATGAAGATCT GGACACCTTT
    AAGCTCCTAG AGGAGGAGGA CTTGGACGAG TTAAATATCA GGGACCCAGA GCACAGAGCT
    GTTCTCTTGA CAGCAGTGGA GTTGTTACAG GAATATGACA GTAACAGCGA CCAGTCCGGA
    TCCCAGGAGA AGCTGCTTGT TGACAGCCAG GGCTTGAGCG GATGCTCTCC ACGAGACTCA
    GGATGCTATG AAAGCAGTGA GAATTTGGAA AACGGCAAGA CTCGGAAAAC CGGCCTCTCT
    GCCAAGTCAT CAACCGAGTC CAGCTTGAAG TCTTTCAACA GGAACCAGCT GGGCAACTAC
    CCAACCTTGC CTTTGACGAA GTCAGTGGAC GCTTTGAAGC AGGGAGGGGA GGGGAGGCTG
    GGCGGTGGTC TCACCCCTCT CGCCTCCAAG AGCTGTGATC CCCCGTGTGT GACTGATCTG
    AATAAAAACC GAAGGAGTCT CCCCGTTTCC ATCTGCCGGA GCTGTGAGAC CCTAGAGGGC
    CCACAGACCG TGGAGACTTG GCCCCGGTCC CACTCCCTGG ACAACCTTCA AGGAGAGCCT
    GAGAAAGATG TGCCTGGCGA GGTGACAGAA CCCTCTCCTG AGATTGTACC TGAAGTGCCA
    CAAAAGACAG CCCCCTCCGT CACAAAGGTT CCAAGCCCCA AACGAGATTC TGCTGTTGAC
    AATGCACTGC TACTGACCCA AAGCAAGAGA TTTTCTGAAC CTCAGAAAAC GACTGCTAAG
    AAACTGGATG GCCCAATGGG GCCCTTTTCA CGGGGTGCAT CCCCCCCTCC ATGTTTGCCC
    AAAACCTTTG ACACCCAGCC TCCTGCCATT AAACCCAGTT TAACACGGAC GCCTCTGGAG
    GGTCATAGGA AAGGACTCGA TTTCGAAGGA ACACATCACC CCCCGGGCAC CAAAGAAGGC
    ATGGACGCTG AGCAGAGGGG CCCCGAGGTC AGAACGCAGG CCAAACCTCC TGTCCAGCCT
    CCGCCCGTTC CCGCCAAGAA GAGCAGGGAG CGCCTCGCCA ACGGGCTGCA TCCTGTCCCT
    CCTGGTCCCC CCGGCGTCCC CAGCCCCGAC GCACCCGGCC TGCCGCTTAA AAAGGGCAGC
    CCTGGCGGCC CCAGTGACTG TCCCCTGGTG GTGGCGGTGG CCAGGCCTCC CCCGGGGCCG
    GAGCCAGGCA GCCCACCAAG CACCAGGCCA CCACCCTGGC TGTCGGAGCT GCCCGAGAGC
    GCCAGCCTCC AGGAGCACGG CGTGCGGCTC GGGCCCGCGC TGGCCAGGAA GGTCTCCTGC
    GCCCGCGGGC TGGACCTAGA AATGCTCACT GAGAACAAGC TGCGGGCGGA AGGCATTGAC
    CTCACCGAGG AGCCCTATTC TGATAAGCAC GGCCGCTGTG GAATTCCTGA GGCCCTGGTG
    CAGAGATATG CAGAAGACTT AGACCAGCCG GAAAGGGATG TCGCCACCAA CATGGACCAG
    ATCCGTGTCA AGCTGCTTCG GAAGCAGCAC CGCATGGCGA TCCCAAGCGG TGGACTCACA
    GAAATCTGCA GGAAGCCCCT CTCTCCCGGC TACGTGTCGT CCGTGTCAGA TTGGCTGGTT
    TCCATCGGTC TGCCCATGTA TGCCAGCGCC CTCTCTGAGG CGGGCTTTAG CACCCTGGGC
    CAAGTGCCTT CTCTGTCCCA CACTTGCCTT CAGGAGGCTG GCATCACAGA GGAGAGACAC
    ATAAGCAAGC TCGTGTCTGC AGCCAGACTC TTCAAACTGC CACCAGGCCC GGAGGCCATG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320336.1
    CDS324..3926
    Translation

    Target ORF information:

    RefSeq Version XM_015464850.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464850.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGGATC AAGATGGCAG GGATGGCTCC CTGGGAAACA TCGATGACCT GGCTCAGCAG 
    TATGCAGATT ACTACAATAC CTGCTTCTCC GACGTGTGTG AGAGGATGGA GGAGCTGCGG
    AAACGGCGGG TTTCCCAGGA CCTGGACGTG GAGAAACCCG AAGCCAGCCC CACGTCACTT
    CAGCTGCGGT CTCAGATCGA AGAGTCGCTT GGCTTCTGTA GCGCTGTATC AACACCAGAA
    GTGGAAAGAA AGAATCCTCT CCATAAGTCA AACTCGGAAG ACAGCTCTGT AGGAAAGGGA
    GATTGGAAGA AGAAAAATAA GTATTTCTGG CAGAACTTCC GAAAGAATCA GAAAGGAATA
    ATGAGACAGA CTTCAAAAGG AGAAGATGTG GGTTACGTTG CAAGCGAGAT AACAATGAGT
    GATGAGGAAC GGATTCAGTT GATGATGATG GTCAAGGAAA AGATGATCAC CATCGAAGAA
    GCGCTTGCTC GGCTCAAGGA GTATGAGGCC CAGCACCGGC AGTCCACTGC CTTGGATCCT
    GCCAACTGGC CGGACGGTTC TTACCCAACA TTTGAAGGCT CATCAAACTG CAATTCAAGA
    GAACAGTCGG AGGATGAGAC GGAGGAGTCG GTGAAGTTTA AGAGGTTACA CAAGCTGGTA
    AACTCCACTC GCAGAGTGAG AAAGAAACTC ATCAGGGTGG AAGAAATGAA AAAGCCCAGC
    ACAGAAGGCG GGGAGGAACA GGTGTTTGAG AATTCCCCGG TCCCAGATGA AAGGTCCGCC
    CTGTACTCCG GCGTGCACAA GAAGCCCTTT TTCTTTGATG CTTCTCCTGA GAAACCCCCA
    GAAGATGACT CAGACTCTCT CACCACTGCT CCATCGTCCA GTAGCCTGGA CACCTGGGGT
    GCCGGCCGCA AATTGGTCAA AACCTTCAGC AAAGGAGAGA GCCGGGGCCT GATCAAGCCC
    CCCAAGAAGA TGGGGACGTT CTTCTCGTAC CCGGAAGACG ACAAGGCCCA GAAGGTGTCC
    CGCTCCCTCA CCGAAGGGGA GATGAAGAAG GGGCTTGGGT CCCTGAGCCA CGGGAGAACC
    TGCAGTTTTG GAGGATTTGA CTTGACAAAT CGTTCTCTGC ACATTGGCAG TAATAATTCG
    GACCCAATGG GTAAAGAAGG AGATTTTGTG TATAAAGAAG TAATCAAATC ACCCACTGCC
    TCCCGAATCT CTCTTGGAAA AAAGGTGAAA TCCGTGAAAG AGACAATGAG GAAGAGAATG
    TCTAAAAAAT ATAGCAGCTC TGTCTCTGAG CAGGATTCAG GCCTTGATGG GATGCCGGGC
    TCCCCTCCGT CCTCTCAGCC TGACAGTGAA CACATGGACA AGCCCAAGCT CAAAGCCGGA
    GGTTCTGTGG AAAGTCTGCG GAGTTCTCTG AGCGGTCAAA GCTCGATGAG TGGTCAGACA
    GTGAGCACCA CCGATTCCTC GACCAGCAAT AGGGAGAGTG TCAAGTCGGA AGATGGTGAT
    GACGAGGAGC CCCCGTACCG GGGCCCGTTC TGTGGGCGTG CCAGGGTGCA CACCGACTTC
    ACGCCCAGCC CCTACGACAC AGACTCCCTC AAGCTCAAGA AAGGAGACAT CATTGATATA
    ATCAGCAAAC CCCCCATGGG CACCTGGATG GGCCTGCTGA ACAACAAGGT GGGCACGTTC
    AAATTCATCT ACGTGGATGT GCTCAACGAG GAAGAGGAGA AGCCCAAGCG CGCCACCAGG
    AGGAGGAGGA AAGGGAGACC CCCTCAGCCG AAGTCAGTGG AGGACCTCCT GGATCGGATC
    AACCTCAAAG AGCACATGCC CACTTTCCTG TTCAATGGCT ATGAAGATCT GGACACCTTT
    AAGCTCCTAG AGGAGGAGGA CTTGGACGAG TTAAATATCA GGGACCCAGA GCACAGAGCT
    GTTCTCTTGA CAGCAGTGGA GTTGTTACAG GAATATGACA GTAACAGCGA CCAGTCCGGA
    TCCCAGGAGA AGCTGCTTGT TGACAGCCAG GGCTTGAGCG GATGCTCTCC ACGAGACTCA
    GGATGCTATG AAAGCAGTGA GAATTTGGAA AACGGCAAGA CTCGGAAAAC CGGCCTCTCT
    GCCAAGTCAT CAACCGAGTC CAGCTTGAAG TCTTTCAACA GGAACCAGCT GGGCAACTAC
    CCAACCTTGC CTTTGACGAA GTCAGTGGAC GCTTTGAAGC AGGGAGGGGA GGGGAGGCTG
    GGCGGTGGTC TCACCCCTCT CGCCTCCAAG AGCTGTGATC CCCCGTGTGT GACTGATCTG
    AATAAAAACC GAAGGAGTCT CCCCGTTTCC ATCTGCCGGA GCTGTGAGAC CCTAGAGGGC
    CCACAGACCG TGGAGACTTG GCCCCGGTCC CACTCCCTGG ACAACCTTCA AGGAGAGCCT
    GAGAAAGATG TGCCTGGCGA GGTGACAGAA CCCTCTCCTG AGATTGTACC TGAAGTGCCA
    CAAAAGACAG CCCCCTCCGT CACAAAGGTT CCAAGCCCCA AACGAGATTC TGCTGTTGAC
    AATGCACTGC TACTGACCCA AAGCAAGAGA TTTTCTGAAC CTCAGAAAAC GACTGCTAAG
    AAACTGGATG GCCCAATGGG GCCCTTTTCA CGGGGTGCAT CCCCCCCTCC ATGTTTGCCC
    AAAACCTTTG ACACCCAGCC TCCTGCCATT AAACCCAGTT TAACACGGAC GCCTCTGGAG
    GGTCATAGGA AAGGACTCGA TTTCGAAGGA ACACATCACC CCCCGGGCAC CAAAGAAGGC
    ATGGACGCTG AGCAGAGGGG CCCCGAGGTC AGAACGCAGG CCAAACCTCC TGTCCAGCCT
    CCGCCCGTTC CCGCCAAGAA GAGCAGGGAG CGCCTCGCCA ACGGGCTGCA TCCTGTCCCT
    CCTGGTCCCC CCGGCGTCCC CAGCCCCGAC GCACCCGGCC TGCCGCTTAA AAAGGGCAGC
    CCTGGCGGCC CCAGTGACTG TCCCCTGGTG GTGGCGGTGG CCAGGCCTCC CCCGGGGCCG
    GAGCCAGGCA GCCCACCAAG CACCAGGCCA CCACCCTGGC TGTCGGAGCT GCCCGAGAGC
    GCCAGCCTCC AGGAGCACGG CGTGCGGCTC GGGCCCGCGC TGGCCAGGAA GGTCTCCTGC
    GCCCGCGGGC TGGACCTAGA AATGCTCACT GAGAACAAGC TGCGGGCGGA AGGCATTGAC
    CTCACCGAGG AGCCCTATTC TGATAAGCAC GGCCGCTGTG GAATTCCTGA GGCCCTGGTG
    CAGAGATATG CAGAAGACTT AGACCAGCCG GAAAGGGATG TCGCCACCAA CATGGACCAG
    ATCCGTGTCA AGCTGCTTCG GAAGCAGCAC CGCATGGCGA TCCCAAGCGG TGGACTCACA
    GAAATCTGCA GGAAGCCCCT CTCTCCCGGC TACGTGTCGT CCGTGTCAGA TTGGCTGGTT
    TCCATCGGTC TGCCCATGTA TGCCAGCGCC CTCTCTGAGG CGGGCTTTAG CACCCTGGGC
    CAAGTGCCTT CTCTGTCCCA CACTTGCCTT CAGGAGGCTG GCATCACAGA GGAGAGACAC
    ATAAGCAAGC TCGTGTCTGC AGCCAGACTC TTCAAACTGC CACCAGGCCC GGAGGCCATG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91514
    Clone ID Related Accession (Same CDS sequence) XM_015464850.1 , XM_015472900.1
    Accession Version XM_015472900.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3603bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product SAM and SH3 domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104231.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGGATC AAGATGGCAG GGATGGCTCC CTGGGAAACA TCGATGACCT GGCTCAGCAG 
    TATGCAGATT ACTACAATAC CTGCTTCTCC GACGTGTGTG AGAGGATGGA GGAGCTGCGG
    AAACGGCGGG TTTCCCAGGA CCTGGACGTG GAGAAACCCG AAGCCAGCCC CACGTCACTT
    CAGCTGCGGT CTCAGATCGA AGAGTCGCTT GGCTTCTGTA GCGCTGTATC AACACCAGAA
    GTGGAAAGAA AGAATCCTCT CCATAAGTCA AACTCGGAAG ACAGCTCTGT AGGAAAGGGA
    GATTGGAAGA AGAAAAATAA GTATTTCTGG CAGAACTTCC GAAAGAATCA GAAAGGAATA
    ATGAGACAGA CTTCAAAAGG AGAAGATGTG GGTTACGTTG CAAGCGAGAT AACAATGAGT
    GATGAGGAAC GGATTCAGTT GATGATGATG GTCAAGGAAA AGATGATCAC CATCGAAGAA
    GCGCTTGCTC GGCTCAAGGA GTATGAGGCC CAGCACCGGC AGTCCACTGC CTTGGATCCT
    GCCAACTGGC CGGACGGTTC TTACCCAACA TTTGAAGGCT CATCAAACTG CAATTCAAGA
    GAACAGTCGG AGGATGAGAC GGAGGAGTCG GTGAAGTTTA AGAGGTTACA CAAGCTGGTA
    AACTCCACTC GCAGAGTGAG AAAGAAACTC ATCAGGGTGG AAGAAATGAA AAAGCCCAGC
    ACAGAAGGCG GGGAGGAACA GGTGTTTGAG AATTCCCCGG TCCCAGATGA AAGGTCCGCC
    CTGTACTCCG GCGTGCACAA GAAGCCCTTT TTCTTTGATG CTTCTCCTGA GAAACCCCCA
    GAAGATGACT CAGACTCTCT CACCACTGCT CCATCGTCCA GTAGCCTGGA CACCTGGGGT
    GCCGGCCGCA AATTGGTCAA AACCTTCAGC AAAGGAGAGA GCCGGGGCCT GATCAAGCCC
    CCCAAGAAGA TGGGGACGTT CTTCTCGTAC CCGGAAGACG ACAAGGCCCA GAAGGTGTCC
    CGCTCCCTCA CCGAAGGGGA GATGAAGAAG GGGCTTGGGT CCCTGAGCCA CGGGAGAACC
    TGCAGTTTTG GAGGATTTGA CTTGACAAAT CGTTCTCTGC ACATTGGCAG TAATAATTCG
    GACCCAATGG GTAAAGAAGG AGATTTTGTG TATAAAGAAG TAATCAAATC ACCCACTGCC
    TCCCGAATCT CTCTTGGAAA AAAGGTGAAA TCCGTGAAAG AGACAATGAG GAAGAGAATG
    TCTAAAAAAT ATAGCAGCTC TGTCTCTGAG CAGGATTCAG GCCTTGATGG GATGCCGGGC
    TCCCCTCCGT CCTCTCAGCC TGACAGTGAA CACATGGACA AGCCCAAGCT CAAAGCCGGA
    GGTTCTGTGG AAAGTCTGCG GAGTTCTCTG AGCGGTCAAA GCTCGATGAG TGGTCAGACA
    GTGAGCACCA CCGATTCCTC GACCAGCAAT AGGGAGAGTG TCAAGTCGGA AGATGGTGAT
    GACGAGGAGC CCCCGTACCG GGGCCCGTTC TGTGGGCGTG CCAGGGTGCA CACCGACTTC
    ACGCCCAGCC CCTACGACAC AGACTCCCTC AAGCTCAAGA AAGGAGACAT CATTGATATA
    ATCAGCAAAC CCCCCATGGG CACCTGGATG GGCCTGCTGA ACAACAAGGT GGGCACGTTC
    AAATTCATCT ACGTGGATGT GCTCAACGAG GAAGAGGAGA AGCCCAAGCG CGCCACCAGG
    AGGAGGAGGA AAGGGAGACC CCCTCAGCCG AAGTCAGTGG AGGACCTCCT GGATCGGATC
    AACCTCAAAG AGCACATGCC CACTTTCCTG TTCAATGGCT ATGAAGATCT GGACACCTTT
    AAGCTCCTAG AGGAGGAGGA CTTGGACGAG TTAAATATCA GGGACCCAGA GCACAGAGCT
    GTTCTCTTGA CAGCAGTGGA GTTGTTACAG GAATATGACA GTAACAGCGA CCAGTCCGGA
    TCCCAGGAGA AGCTGCTTGT TGACAGCCAG GGCTTGAGCG GATGCTCTCC ACGAGACTCA
    GGATGCTATG AAAGCAGTGA GAATTTGGAA AACGGCAAGA CTCGGAAAAC CGGCCTCTCT
    GCCAAGTCAT CAACCGAGTC CAGCTTGAAG TCTTTCAACA GGAACCAGCT GGGCAACTAC
    CCAACCTTGC CTTTGACGAA GTCAGTGGAC GCTTTGAAGC AGGGAGGGGA GGGGAGGCTG
    GGCGGTGGTC TCACCCCTCT CGCCTCCAAG AGCTGTGATC CCCCGTGTGT GACTGATCTG
    AATAAAAACC GAAGGAGTCT CCCCGTTTCC ATCTGCCGGA GCTGTGAGAC CCTAGAGGGC
    CCACAGACCG TGGAGACTTG GCCCCGGTCC CACTCCCTGG ACAACCTTCA AGGAGAGCCT
    GAGAAAGATG TGCCTGGCGA GGTGACAGAA CCCTCTCCTG AGATTGTACC TGAAGTGCCA
    CAAAAGACAG CCCCCTCCGT CACAAAGGTT CCAAGCCCCA AACGAGATTC TGCTGTTGAC
    AATGCACTGC TACTGACCCA AAGCAAGAGA TTTTCTGAAC CTCAGAAAAC GACTGCTAAG
    AAACTGGATG GCCCAATGGG GCCCTTTTCA CGGGGTGCAT CCCCCCCTCC ATGTTTGCCC
    AAAACCTTTG ACACCCAGCC TCCTGCCATT AAACCCAGTT TAACACGGAC GCCTCTGGAG
    GGTCATAGGA AAGGACTCGA TTTCGAAGGA ACACATCACC CCCCGGGCAC CAAAGAAGGC
    ATGGACGCTG AGCAGAGGGG CCCCGAGGTC AGAACGCAGG CCAAACCTCC TGTCCAGCCT
    CCGCCCGTTC CCGCCAAGAA GAGCAGGGAG CGCCTCGCCA ACGGGCTGCA TCCTGTCCCT
    CCTGGTCCCC CCGGCGTCCC CAGCCCCGAC GCACCCGGCC TGCCGCTTAA AAAGGGCAGC
    CCTGGCGGCC CCAGTGACTG TCCCCTGGTG GTGGCGGTGG CCAGGCCTCC CCCGGGGCCG
    GAGCCAGGCA GCCCACCAAG CACCAGGCCA CCACCCTGGC TGTCGGAGCT GCCCGAGAGC
    GCCAGCCTCC AGGAGCACGG CGTGCGGCTC GGGCCCGCGC TGGCCAGGAA GGTCTCCTGC
    GCCCGCGGGC TGGACCTAGA AATGCTCACT GAGAACAAGC TGCGGGCGGA AGGCATTGAC
    CTCACCGAGG AGCCCTATTC TGATAAGCAC GGCCGCTGTG GAATTCCTGA GGCCCTGGTG
    CAGAGATATG CAGAAGACTT AGACCAGCCG GAAAGGGATG TCGCCACCAA CATGGACCAG
    ATCCGTGTCA AGCTGCTTCG GAAGCAGCAC CGCATGGCGA TCCCAAGCGG TGGACTCACA
    GAAATCTGCA GGAAGCCCCT CTCTCCCGGC TACGTGTCGT CCGTGTCAGA TTGGCTGGTT
    TCCATCGGTC TGCCCATGTA TGCCAGCGCC CTCTCTGAGG CGGGCTTTAG CACCCTGGGC
    CAAGTGCCTT CTCTGTCCCA CACTTGCCTT CAGGAGGCTG GCATCACAGA GGAGAGACAC
    ATAAGCAAGC TCGTGTCTGC AGCCAGACTC TTCAAACTGC CACCAGGCCC GGAGGCCATG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328386.1
    CDS324..3926
    Translation

    Target ORF information:

    RefSeq Version XM_015472900.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472900.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGGATC AAGATGGCAG GGATGGCTCC CTGGGAAACA TCGATGACCT GGCTCAGCAG 
    TATGCAGATT ACTACAATAC CTGCTTCTCC GACGTGTGTG AGAGGATGGA GGAGCTGCGG
    AAACGGCGGG TTTCCCAGGA CCTGGACGTG GAGAAACCCG AAGCCAGCCC CACGTCACTT
    CAGCTGCGGT CTCAGATCGA AGAGTCGCTT GGCTTCTGTA GCGCTGTATC AACACCAGAA
    GTGGAAAGAA AGAATCCTCT CCATAAGTCA AACTCGGAAG ACAGCTCTGT AGGAAAGGGA
    GATTGGAAGA AGAAAAATAA GTATTTCTGG CAGAACTTCC GAAAGAATCA GAAAGGAATA
    ATGAGACAGA CTTCAAAAGG AGAAGATGTG GGTTACGTTG CAAGCGAGAT AACAATGAGT
    GATGAGGAAC GGATTCAGTT GATGATGATG GTCAAGGAAA AGATGATCAC CATCGAAGAA
    GCGCTTGCTC GGCTCAAGGA GTATGAGGCC CAGCACCGGC AGTCCACTGC CTTGGATCCT
    GCCAACTGGC CGGACGGTTC TTACCCAACA TTTGAAGGCT CATCAAACTG CAATTCAAGA
    GAACAGTCGG AGGATGAGAC GGAGGAGTCG GTGAAGTTTA AGAGGTTACA CAAGCTGGTA
    AACTCCACTC GCAGAGTGAG AAAGAAACTC ATCAGGGTGG AAGAAATGAA AAAGCCCAGC
    ACAGAAGGCG GGGAGGAACA GGTGTTTGAG AATTCCCCGG TCCCAGATGA AAGGTCCGCC
    CTGTACTCCG GCGTGCACAA GAAGCCCTTT TTCTTTGATG CTTCTCCTGA GAAACCCCCA
    GAAGATGACT CAGACTCTCT CACCACTGCT CCATCGTCCA GTAGCCTGGA CACCTGGGGT
    GCCGGCCGCA AATTGGTCAA AACCTTCAGC AAAGGAGAGA GCCGGGGCCT GATCAAGCCC
    CCCAAGAAGA TGGGGACGTT CTTCTCGTAC CCGGAAGACG ACAAGGCCCA GAAGGTGTCC
    CGCTCCCTCA CCGAAGGGGA GATGAAGAAG GGGCTTGGGT CCCTGAGCCA CGGGAGAACC
    TGCAGTTTTG GAGGATTTGA CTTGACAAAT CGTTCTCTGC ACATTGGCAG TAATAATTCG
    GACCCAATGG GTAAAGAAGG AGATTTTGTG TATAAAGAAG TAATCAAATC ACCCACTGCC
    TCCCGAATCT CTCTTGGAAA AAAGGTGAAA TCCGTGAAAG AGACAATGAG GAAGAGAATG
    TCTAAAAAAT ATAGCAGCTC TGTCTCTGAG CAGGATTCAG GCCTTGATGG GATGCCGGGC
    TCCCCTCCGT CCTCTCAGCC TGACAGTGAA CACATGGACA AGCCCAAGCT CAAAGCCGGA
    GGTTCTGTGG AAAGTCTGCG GAGTTCTCTG AGCGGTCAAA GCTCGATGAG TGGTCAGACA
    GTGAGCACCA CCGATTCCTC GACCAGCAAT AGGGAGAGTG TCAAGTCGGA AGATGGTGAT
    GACGAGGAGC CCCCGTACCG GGGCCCGTTC TGTGGGCGTG CCAGGGTGCA CACCGACTTC
    ACGCCCAGCC CCTACGACAC AGACTCCCTC AAGCTCAAGA AAGGAGACAT CATTGATATA
    ATCAGCAAAC CCCCCATGGG CACCTGGATG GGCCTGCTGA ACAACAAGGT GGGCACGTTC
    AAATTCATCT ACGTGGATGT GCTCAACGAG GAAGAGGAGA AGCCCAAGCG CGCCACCAGG
    AGGAGGAGGA AAGGGAGACC CCCTCAGCCG AAGTCAGTGG AGGACCTCCT GGATCGGATC
    AACCTCAAAG AGCACATGCC CACTTTCCTG TTCAATGGCT ATGAAGATCT GGACACCTTT
    AAGCTCCTAG AGGAGGAGGA CTTGGACGAG TTAAATATCA GGGACCCAGA GCACAGAGCT
    GTTCTCTTGA CAGCAGTGGA GTTGTTACAG GAATATGACA GTAACAGCGA CCAGTCCGGA
    TCCCAGGAGA AGCTGCTTGT TGACAGCCAG GGCTTGAGCG GATGCTCTCC ACGAGACTCA
    GGATGCTATG AAAGCAGTGA GAATTTGGAA AACGGCAAGA CTCGGAAAAC CGGCCTCTCT
    GCCAAGTCAT CAACCGAGTC CAGCTTGAAG TCTTTCAACA GGAACCAGCT GGGCAACTAC
    CCAACCTTGC CTTTGACGAA GTCAGTGGAC GCTTTGAAGC AGGGAGGGGA GGGGAGGCTG
    GGCGGTGGTC TCACCCCTCT CGCCTCCAAG AGCTGTGATC CCCCGTGTGT GACTGATCTG
    AATAAAAACC GAAGGAGTCT CCCCGTTTCC ATCTGCCGGA GCTGTGAGAC CCTAGAGGGC
    CCACAGACCG TGGAGACTTG GCCCCGGTCC CACTCCCTGG ACAACCTTCA AGGAGAGCCT
    GAGAAAGATG TGCCTGGCGA GGTGACAGAA CCCTCTCCTG AGATTGTACC TGAAGTGCCA
    CAAAAGACAG CCCCCTCCGT CACAAAGGTT CCAAGCCCCA AACGAGATTC TGCTGTTGAC
    AATGCACTGC TACTGACCCA AAGCAAGAGA TTTTCTGAAC CTCAGAAAAC GACTGCTAAG
    AAACTGGATG GCCCAATGGG GCCCTTTTCA CGGGGTGCAT CCCCCCCTCC ATGTTTGCCC
    AAAACCTTTG ACACCCAGCC TCCTGCCATT AAACCCAGTT TAACACGGAC GCCTCTGGAG
    GGTCATAGGA AAGGACTCGA TTTCGAAGGA ACACATCACC CCCCGGGCAC CAAAGAAGGC
    ATGGACGCTG AGCAGAGGGG CCCCGAGGTC AGAACGCAGG CCAAACCTCC TGTCCAGCCT
    CCGCCCGTTC CCGCCAAGAA GAGCAGGGAG CGCCTCGCCA ACGGGCTGCA TCCTGTCCCT
    CCTGGTCCCC CCGGCGTCCC CAGCCCCGAC GCACCCGGCC TGCCGCTTAA AAAGGGCAGC
    CCTGGCGGCC CCAGTGACTG TCCCCTGGTG GTGGCGGTGG CCAGGCCTCC CCCGGGGCCG
    GAGCCAGGCA GCCCACCAAG CACCAGGCCA CCACCCTGGC TGTCGGAGCT GCCCGAGAGC
    GCCAGCCTCC AGGAGCACGG CGTGCGGCTC GGGCCCGCGC TGGCCAGGAA GGTCTCCTGC
    GCCCGCGGGC TGGACCTAGA AATGCTCACT GAGAACAAGC TGCGGGCGGA AGGCATTGAC
    CTCACCGAGG AGCCCTATTC TGATAAGCAC GGCCGCTGTG GAATTCCTGA GGCCCTGGTG
    CAGAGATATG CAGAAGACTT AGACCAGCCG GAAAGGGATG TCGCCACCAA CATGGACCAG
    ATCCGTGTCA AGCTGCTTCG GAAGCAGCAC CGCATGGCGA TCCCAAGCGG TGGACTCACA
    GAAATCTGCA GGAAGCCCCT CTCTCCCGGC TACGTGTCGT CCGTGTCAGA TTGGCTGGTT
    TCCATCGGTC TGCCCATGTA TGCCAGCGCC CTCTCTGAGG CGGGCTTTAG CACCCTGGGC
    CAAGTGCCTT CTCTGTCCCA CACTTGCCTT CAGGAGGCTG GCATCACAGA GGAGAGACAC
    ATAAGCAAGC TCGTGTCTGC AGCCAGACTC TTCAAACTGC CACCAGGCCC GGAGGCCATG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06173
    Clone ID Related Accession (Same CDS sequence) XM_005211030.4 , XM_005199430.3 , XM_005211030.3
    Accession Version XM_005211030.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product SAM and SH3 domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211030.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCCGAAGC CGTCAAGAGA ACAGTCGGAG GATGAGACGG AGGAGTCGGT GAAGTTTAAG 
    AGGTTACACA AGCTGGTAAA CTCCACTCGC AGAGTGAGAA AGAAACTCAT CAGGGTGGAA
    GAAATGAAAA AGCCCAGCAC AGAAGGCGGG GAGGAACAGG TGTTTGAGAA TTCCCCGGTC
    CCAGATGAAA GGTCCGCCCT GTACTCCGGC GTGCACAAGA AGCCCTTTTT CTTTGATGCT
    TCTCCTGAGA AACCCCCAGA AGATGACTCA GACTCTCTCA CCACTGCTCC ATCGTCCAGT
    AGCCTGGACA CCTGGGGTGC CGGCCGCAAA TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC
    CGGGGCCTGA TCAAGCCCCC CAAGAAGATG GGGACGTTCT TCTCGTACCC GGAAGACGAC
    AAGGCCCAGA AGGTGTCCCG CTCCCTCACC GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC
    CTGAGCCACG GGAGAACCTG CAGTTTTGGA GGATTTGACT TGACAAATCG TTCTCTGCAC
    ATTGGCAGTA ATAATTCGGA CCCAATGGGT AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA
    ATCAAATCAC CCACTGCCTC CCGAATCTCT CTTGGAAAAA AGGTGAAATC CGTGAAAGAG
    ACAATGAGGA AGAGAATGTC TAAAAAATAT AGCAGCTCTG TCTCTGAGCA GGATTCAGGC
    CTTGATGGGA TGCCGGGCTC CCCTCCGTCC TCTCAGCCTG ACAGTGAACA CATGGACAAG
    CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC
    TCGATGAGTG GTCAGACAGT GAGCACCACC GATTCCTCGA CCAGCAATAG GGAGAGTGTC
    AAGTCGGAAG ATGGTGATGA CGAGGAGCCC CCGTACCGGG GCCCGTTCTG TGGGCGTGCC
    AGGGTGCACA CCGACTTCAC GCCCAGCCCC TACGACACAG ACTCCCTCAA GCTCAAGAAA
    GGAGACATCA TTGATATAAT CAGCAAACCC CCCATGGGCA CCTGGATGGG CCTGCTGAAC
    AACAAGGTGG GCACGTTCAA ATTCATCTAC GTGGATGTGC TCAACGAGGA AGAGGAGAAG
    CCCAAGCGCG CCACCAGGAG GAGGAGGAAA GGGAGACCCC CTCAGCCGAA GTCAGTGGAG
    GACCTCCTGG ATCGGATCAA CCTCAAAGAG CACATGCCCA CTTTCCTGTT CAATGGCTAT
    GAAGATCTGG ACACCTTTAA GCTCCTAGAG GAGGAGGACT TGGACGAGTT AAATATCAGG
    GACCCAGAGC ACAGAGCTGT TCTCTTGACA GCAGTGGAGT TGTTACAGGA ATATGACAGT
    AACAGCGACC AGTCCGGATC CCAGGAGAAG CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA
    TGCTCTCCAC GAGACTCAGG ATGCTATGAA AGCAGTGAGA ATTTGGAAAA CGGCAAGACT
    CGGAAAACCG GCCTCTCTGC CAAGTCATCA ACCGAGTCCA GCTTGAAGTC TTTCAACAGG
    AACCAGCTGG GCAACTACCC AACCTTGCCT TTGACGAAGT CAGTGGACGC TTTGAAGCAG
    GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC ACCCCTCTCG CCTCCAAGAG CTGTGATCCC
    CCGTGTGTGA CTGATCTGAA TAAAAACCGA AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC
    TGTGAGACCC TAGAGGGCCC ACAGACCGTG GAGACTTGGC CCCGGTCCCA CTCCCTGGAC
    AACCTTCAAG GAGAGCCTGA GAAAGATGTG CCTGGCGAGG TGACAGAACC CTCTCCTGAG
    ATTGTACCTG AAGTGCCACA AAAGACAGCC CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA
    CGAGATTCTG CTGTTGACAA TGCACTGCTA CTGACCCAAA GCAAGAGATT TTCTGAACCT
    CAGAAAACGA CTGCTAAGAA ACTGGATGGC CCAATGGGGC CCTTTTCACG GGGTGCATCC
    CCCCCTCCAT GTTTGCCCAA AACCTTTGAC ACCCAGCCTC CTGCCATTAA ACCCAGTTTA
    ACACGGACGC CTCTGGAGGG TCATAGGAAA GGACTCGATT TCGAAGGAAC ACATCACCCC
    CCGGGCACCA AAGAAGGCAT GGACGCTGAG CAGAGGGGCC CCGAGGTCAG AACGCAGGCC
    AAACCTCCTG TCCAGCCTCC GCCCGTTCCC GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC
    GGGCTGCATC CTGTCCCTCC TGGTCCCCCC GGCGTCCCCA GCCCCGACGC ACCCGGCCTG
    CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC AGTGACTGTC CCCTGGTGGT GGCGGTGGCC
    AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC CCACCAAGCA CCAGGCCACC ACCCTGGCTG
    TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG GAGCACGGCG TGCGGCTCGG GCCCGCGCTG
    GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG GACCTAGAAA TGCTCACTGA GAACAAGCTG
    CGGGCGGAAG GCATTGACCT CACCGAGGAG CCCTATTCTG ATAAGCACGG CCGCTGTGGA
    ATTCCTGAGG CCCTGGTGCA GAGATATGCA GAAGACTTAG ACCAGCCGGA AAGGGATGTC
    GCCACCAACA TGGACCAGAT CCGTGTCAAG CTGCTTCGGA AGCAGCACCG CATGGCGATC
    CCAAGCGGTG GACTCACAGA AATCTGCAGG AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC
    GTGTCAGATT GGCTGGTTTC CATCGGTCTG CCCATGTATG CCAGCGCCCT CTCTGAGGCG
    GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT CTGTCCCACA CTTGCCTTCA GGAGGCTGGC
    ATCACAGAGG AGAGACACAT AAGCAAGCTC GTGTCTGCAG CCAGACTCTT CAAACTGCCA
    CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211087.1
    CDS1239..4259
    Translation

    Target ORF information:

    RefSeq Version XM_005211030.4
    Organism Bos taurus(cattle)
    Definition Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211030.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCCGAAGC CGTCAAGAGA ACAGTCGGAG GATGAGACGG AGGAGTCGGT GAAGTTTAAG 
    AGGTTACACA AGCTGGTAAA CTCCACTCGC AGAGTGAGAA AGAAACTCAT CAGGGTGGAA
    GAAATGAAAA AGCCCAGCAC AGAAGGCGGG GAGGAACAGG TGTTTGAGAA TTCCCCGGTC
    CCAGATGAAA GGTCCGCCCT GTACTCCGGC GTGCACAAGA AGCCCTTTTT CTTTGATGCT
    TCTCCTGAGA AACCCCCAGA AGATGACTCA GACTCTCTCA CCACTGCTCC ATCGTCCAGT
    AGCCTGGACA CCTGGGGTGC CGGCCGCAAA TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC
    CGGGGCCTGA TCAAGCCCCC CAAGAAGATG GGGACGTTCT TCTCGTACCC GGAAGACGAC
    AAGGCCCAGA AGGTGTCCCG CTCCCTCACC GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC
    CTGAGCCACG GGAGAACCTG CAGTTTTGGA GGATTTGACT TGACAAATCG TTCTCTGCAC
    ATTGGCAGTA ATAATTCGGA CCCAATGGGT AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA
    ATCAAATCAC CCACTGCCTC CCGAATCTCT CTTGGAAAAA AGGTGAAATC CGTGAAAGAG
    ACAATGAGGA AGAGAATGTC TAAAAAATAT AGCAGCTCTG TCTCTGAGCA GGATTCAGGC
    CTTGATGGGA TGCCGGGCTC CCCTCCGTCC TCTCAGCCTG ACAGTGAACA CATGGACAAG
    CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC
    TCGATGAGTG GTCAGACAGT GAGCACCACC GATTCCTCGA CCAGCAATAG GGAGAGTGTC
    AAGTCGGAAG ATGGTGATGA CGAGGAGCCC CCGTACCGGG GCCCGTTCTG TGGGCGTGCC
    AGGGTGCACA CCGACTTCAC GCCCAGCCCC TACGACACAG ACTCCCTCAA GCTCAAGAAA
    GGAGACATCA TTGATATAAT CAGCAAACCC CCCATGGGCA CCTGGATGGG CCTGCTGAAC
    AACAAGGTGG GCACGTTCAA ATTCATCTAC GTGGATGTGC TCAACGAGGA AGAGGAGAAG
    CCCAAGCGCG CCACCAGGAG GAGGAGGAAA GGGAGACCCC CTCAGCCGAA GTCAGTGGAG
    GACCTCCTGG ATCGGATCAA CCTCAAAGAG CACATGCCCA CTTTCCTGTT CAATGGCTAT
    GAAGATCTGG ACACCTTTAA GCTCCTAGAG GAGGAGGACT TGGACGAGTT AAATATCAGG
    GACCCAGAGC ACAGAGCTGT TCTCTTGACA GCAGTGGAGT TGTTACAGGA ATATGACAGT
    AACAGCGACC AGTCCGGATC CCAGGAGAAG CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA
    TGCTCTCCAC GAGACTCAGG ATGCTATGAA AGCAGTGAGA ATTTGGAAAA CGGCAAGACT
    CGGAAAACCG GCCTCTCTGC CAAGTCATCA ACCGAGTCCA GCTTGAAGTC TTTCAACAGG
    AACCAGCTGG GCAACTACCC AACCTTGCCT TTGACGAAGT CAGTGGACGC TTTGAAGCAG
    GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC ACCCCTCTCG CCTCCAAGAG CTGTGATCCC
    CCGTGTGTGA CTGATCTGAA TAAAAACCGA AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC
    TGTGAGACCC TAGAGGGCCC ACAGACCGTG GAGACTTGGC CCCGGTCCCA CTCCCTGGAC
    AACCTTCAAG GAGAGCCTGA GAAAGATGTG CCTGGCGAGG TGACAGAACC CTCTCCTGAG
    ATTGTACCTG AAGTGCCACA AAAGACAGCC CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA
    CGAGATTCTG CTGTTGACAA TGCACTGCTA CTGACCCAAA GCAAGAGATT TTCTGAACCT
    CAGAAAACGA CTGCTAAGAA ACTGGATGGC CCAATGGGGC CCTTTTCACG GGGTGCATCC
    CCCCCTCCAT GTTTGCCCAA AACCTTTGAC ACCCAGCCTC CTGCCATTAA ACCCAGTTTA
    ACACGGACGC CTCTGGAGGG TCATAGGAAA GGACTCGATT TCGAAGGAAC ACATCACCCC
    CCGGGCACCA AAGAAGGCAT GGACGCTGAG CAGAGGGGCC CCGAGGTCAG AACGCAGGCC
    AAACCTCCTG TCCAGCCTCC GCCCGTTCCC GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC
    GGGCTGCATC CTGTCCCTCC TGGTCCCCCC GGCGTCCCCA GCCCCGACGC ACCCGGCCTG
    CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC AGTGACTGTC CCCTGGTGGT GGCGGTGGCC
    AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC CCACCAAGCA CCAGGCCACC ACCCTGGCTG
    TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG GAGCACGGCG TGCGGCTCGG GCCCGCGCTG
    GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG GACCTAGAAA TGCTCACTGA GAACAAGCTG
    CGGGCGGAAG GCATTGACCT CACCGAGGAG CCCTATTCTG ATAAGCACGG CCGCTGTGGA
    ATTCCTGAGG CCCTGGTGCA GAGATATGCA GAAGACTTAG ACCAGCCGGA AAGGGATGTC
    GCCACCAACA TGGACCAGAT CCGTGTCAAG CTGCTTCGGA AGCAGCACCG CATGGCGATC
    CCAAGCGGTG GACTCACAGA AATCTGCAGG AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC
    GTGTCAGATT GGCTGGTTTC CATCGGTCTG CCCATGTATG CCAGCGCCCT CTCTGAGGCG
    GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT CTGTCCCACA CTTGCCTTCA GGAGGCTGGC
    ATCACAGAGG AGAGACACAT AAGCAAGCTC GTGTCTGCAG CCAGACTCTT CAAACTGCCA
    CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06173
    Clone ID Related Accession (Same CDS sequence) XM_005211030.4 , XM_005199430.3 , XM_005211030.3
    Accession Version XM_005199430.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product SAM and SH3 domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645735.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199430.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCCGAAGC CGTCAAGAGA ACAGTCGGAG GATGAGACGG AGGAGTCGGT GAAGTTTAAG 
    AGGTTACACA AGCTGGTAAA CTCCACTCGC AGAGTGAGAA AGAAACTCAT CAGGGTGGAA
    GAAATGAAAA AGCCCAGCAC AGAAGGCGGG GAGGAACAGG TGTTTGAGAA TTCCCCGGTC
    CCAGATGAAA GGTCCGCCCT GTACTCCGGC GTGCACAAGA AGCCCTTTTT CTTTGATGCT
    TCTCCTGAGA AACCCCCAGA AGATGACTCA GACTCTCTCA CCACTGCTCC ATCGTCCAGT
    AGCCTGGACA CCTGGGGTGC CGGCCGCAAA TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC
    CGGGGCCTGA TCAAGCCCCC CAAGAAGATG GGGACGTTCT TCTCGTACCC GGAAGACGAC
    AAGGCCCAGA AGGTGTCCCG CTCCCTCACC GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC
    CTGAGCCACG GGAGAACCTG CAGTTTTGGA GGATTTGACT TGACAAATCG TTCTCTGCAC
    ATTGGCAGTA ATAATTCGGA CCCAATGGGT AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA
    ATCAAATCAC CCACTGCCTC CCGAATCTCT CTTGGAAAAA AGGTGAAATC CGTGAAAGAG
    ACAATGAGGA AGAGAATGTC TAAAAAATAT AGCAGCTCTG TCTCTGAGCA GGATTCAGGC
    CTTGATGGGA TGCCGGGCTC CCCTCCGTCC TCTCAGCCTG ACAGTGAACA CATGGACAAG
    CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC
    TCGATGAGTG GTCAGACAGT GAGCACCACC GATTCCTCGA CCAGCAATAG GGAGAGTGTC
    AAGTCGGAAG ATGGTGATGA CGAGGAGCCC CCGTACCGGG GCCCGTTCTG TGGGCGTGCC
    AGGGTGCACA CCGACTTCAC GCCCAGCCCC TACGACACAG ACTCCCTCAA GCTCAAGAAA
    GGAGACATCA TTGATATAAT CAGCAAACCC CCCATGGGCA CCTGGATGGG CCTGCTGAAC
    AACAAGGTGG GCACGTTCAA ATTCATCTAC GTGGATGTGC TCAACGAGGA AGAGGAGAAG
    CCCAAGCGCG CCACCAGGAG GAGGAGGAAA GGGAGACCCC CTCAGCCGAA GTCAGTGGAG
    GACCTCCTGG ATCGGATCAA CCTCAAAGAG CACATGCCCA CTTTCCTGTT CAATGGCTAT
    GAAGATCTGG ACACCTTTAA GCTCCTAGAG GAGGAGGACT TGGACGAGTT AAATATCAGG
    GACCCAGAGC ACAGAGCTGT TCTCTTGACA GCAGTGGAGT TGTTACAGGA ATATGACAGT
    AACAGCGACC AGTCCGGATC CCAGGAGAAG CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA
    TGCTCTCCAC GAGACTCAGG ATGCTATGAA AGCAGTGAGA ATTTGGAAAA CGGCAAGACT
    CGGAAAACCG GCCTCTCTGC CAAGTCATCA ACCGAGTCCA GCTTGAAGTC TTTCAACAGG
    AACCAGCTGG GCAACTACCC AACCTTGCCT TTGACGAAGT CAGTGGACGC TTTGAAGCAG
    GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC ACCCCTCTCG CCTCCAAGAG CTGTGATCCC
    CCGTGTGTGA CTGATCTGAA TAAAAACCGA AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC
    TGTGAGACCC TAGAGGGCCC ACAGACCGTG GAGACTTGGC CCCGGTCCCA CTCCCTGGAC
    AACCTTCAAG GAGAGCCTGA GAAAGATGTG CCTGGCGAGG TGACAGAACC CTCTCCTGAG
    ATTGTACCTG AAGTGCCACA AAAGACAGCC CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA
    CGAGATTCTG CTGTTGACAA TGCACTGCTA CTGACCCAAA GCAAGAGATT TTCTGAACCT
    CAGAAAACGA CTGCTAAGAA ACTGGATGGC CCAATGGGGC CCTTTTCACG GGGTGCATCC
    CCCCCTCCAT GTTTGCCCAA AACCTTTGAC ACCCAGCCTC CTGCCATTAA ACCCAGTTTA
    ACACGGACGC CTCTGGAGGG TCATAGGAAA GGACTCGATT TCGAAGGAAC ACATCACCCC
    CCGGGCACCA AAGAAGGCAT GGACGCTGAG CAGAGGGGCC CCGAGGTCAG AACGCAGGCC
    AAACCTCCTG TCCAGCCTCC GCCCGTTCCC GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC
    GGGCTGCATC CTGTCCCTCC TGGTCCCCCC GGCGTCCCCA GCCCCGACGC ACCCGGCCTG
    CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC AGTGACTGTC CCCTGGTGGT GGCGGTGGCC
    AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC CCACCAAGCA CCAGGCCACC ACCCTGGCTG
    TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG GAGCACGGCG TGCGGCTCGG GCCCGCGCTG
    GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG GACCTAGAAA TGCTCACTGA GAACAAGCTG
    CGGGCGGAAG GCATTGACCT CACCGAGGAG CCCTATTCTG ATAAGCACGG CCGCTGTGGA
    ATTCCTGAGG CCCTGGTGCA GAGATATGCA GAAGACTTAG ACCAGCCGGA AAGGGATGTC
    GCCACCAACA TGGACCAGAT CCGTGTCAAG CTGCTTCGGA AGCAGCACCG CATGGCGATC
    CCAAGCGGTG GACTCACAGA AATCTGCAGG AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC
    GTGTCAGATT GGCTGGTTTC CATCGGTCTG CCCATGTATG CCAGCGCCCT CTCTGAGGCG
    GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT CTGTCCCACA CTTGCCTTCA GGAGGCTGGC
    ATCACAGAGG AGAGACACAT AAGCAAGCTC GTGTCTGCAG CCAGACTCTT CAAACTGCCA
    CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199487.1
    CDS1262..4282
    Translation

    Target ORF information:

    RefSeq Version XM_005199430.3
    Organism Bos taurus(cattle)
    Definition Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199430.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCCGAAGC CGTCAAGAGA ACAGTCGGAG GATGAGACGG AGGAGTCGGT GAAGTTTAAG 
    AGGTTACACA AGCTGGTAAA CTCCACTCGC AGAGTGAGAA AGAAACTCAT CAGGGTGGAA
    GAAATGAAAA AGCCCAGCAC AGAAGGCGGG GAGGAACAGG TGTTTGAGAA TTCCCCGGTC
    CCAGATGAAA GGTCCGCCCT GTACTCCGGC GTGCACAAGA AGCCCTTTTT CTTTGATGCT
    TCTCCTGAGA AACCCCCAGA AGATGACTCA GACTCTCTCA CCACTGCTCC ATCGTCCAGT
    AGCCTGGACA CCTGGGGTGC CGGCCGCAAA TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC
    CGGGGCCTGA TCAAGCCCCC CAAGAAGATG GGGACGTTCT TCTCGTACCC GGAAGACGAC
    AAGGCCCAGA AGGTGTCCCG CTCCCTCACC GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC
    CTGAGCCACG GGAGAACCTG CAGTTTTGGA GGATTTGACT TGACAAATCG TTCTCTGCAC
    ATTGGCAGTA ATAATTCGGA CCCAATGGGT AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA
    ATCAAATCAC CCACTGCCTC CCGAATCTCT CTTGGAAAAA AGGTGAAATC CGTGAAAGAG
    ACAATGAGGA AGAGAATGTC TAAAAAATAT AGCAGCTCTG TCTCTGAGCA GGATTCAGGC
    CTTGATGGGA TGCCGGGCTC CCCTCCGTCC TCTCAGCCTG ACAGTGAACA CATGGACAAG
    CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC
    TCGATGAGTG GTCAGACAGT GAGCACCACC GATTCCTCGA CCAGCAATAG GGAGAGTGTC
    AAGTCGGAAG ATGGTGATGA CGAGGAGCCC CCGTACCGGG GCCCGTTCTG TGGGCGTGCC
    AGGGTGCACA CCGACTTCAC GCCCAGCCCC TACGACACAG ACTCCCTCAA GCTCAAGAAA
    GGAGACATCA TTGATATAAT CAGCAAACCC CCCATGGGCA CCTGGATGGG CCTGCTGAAC
    AACAAGGTGG GCACGTTCAA ATTCATCTAC GTGGATGTGC TCAACGAGGA AGAGGAGAAG
    CCCAAGCGCG CCACCAGGAG GAGGAGGAAA GGGAGACCCC CTCAGCCGAA GTCAGTGGAG
    GACCTCCTGG ATCGGATCAA CCTCAAAGAG CACATGCCCA CTTTCCTGTT CAATGGCTAT
    GAAGATCTGG ACACCTTTAA GCTCCTAGAG GAGGAGGACT TGGACGAGTT AAATATCAGG
    GACCCAGAGC ACAGAGCTGT TCTCTTGACA GCAGTGGAGT TGTTACAGGA ATATGACAGT
    AACAGCGACC AGTCCGGATC CCAGGAGAAG CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA
    TGCTCTCCAC GAGACTCAGG ATGCTATGAA AGCAGTGAGA ATTTGGAAAA CGGCAAGACT
    CGGAAAACCG GCCTCTCTGC CAAGTCATCA ACCGAGTCCA GCTTGAAGTC TTTCAACAGG
    AACCAGCTGG GCAACTACCC AACCTTGCCT TTGACGAAGT CAGTGGACGC TTTGAAGCAG
    GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC ACCCCTCTCG CCTCCAAGAG CTGTGATCCC
    CCGTGTGTGA CTGATCTGAA TAAAAACCGA AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC
    TGTGAGACCC TAGAGGGCCC ACAGACCGTG GAGACTTGGC CCCGGTCCCA CTCCCTGGAC
    AACCTTCAAG GAGAGCCTGA GAAAGATGTG CCTGGCGAGG TGACAGAACC CTCTCCTGAG
    ATTGTACCTG AAGTGCCACA AAAGACAGCC CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA
    CGAGATTCTG CTGTTGACAA TGCACTGCTA CTGACCCAAA GCAAGAGATT TTCTGAACCT
    CAGAAAACGA CTGCTAAGAA ACTGGATGGC CCAATGGGGC CCTTTTCACG GGGTGCATCC
    CCCCCTCCAT GTTTGCCCAA AACCTTTGAC ACCCAGCCTC CTGCCATTAA ACCCAGTTTA
    ACACGGACGC CTCTGGAGGG TCATAGGAAA GGACTCGATT TCGAAGGAAC ACATCACCCC
    CCGGGCACCA AAGAAGGCAT GGACGCTGAG CAGAGGGGCC CCGAGGTCAG AACGCAGGCC
    AAACCTCCTG TCCAGCCTCC GCCCGTTCCC GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC
    GGGCTGCATC CTGTCCCTCC TGGTCCCCCC GGCGTCCCCA GCCCCGACGC ACCCGGCCTG
    CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC AGTGACTGTC CCCTGGTGGT GGCGGTGGCC
    AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC CCACCAAGCA CCAGGCCACC ACCCTGGCTG
    TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG GAGCACGGCG TGCGGCTCGG GCCCGCGCTG
    GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG GACCTAGAAA TGCTCACTGA GAACAAGCTG
    CGGGCGGAAG GCATTGACCT CACCGAGGAG CCCTATTCTG ATAAGCACGG CCGCTGTGGA
    ATTCCTGAGG CCCTGGTGCA GAGATATGCA GAAGACTTAG ACCAGCCGGA AAGGGATGTC
    GCCACCAACA TGGACCAGAT CCGTGTCAAG CTGCTTCGGA AGCAGCACCG CATGGCGATC
    CCAAGCGGTG GACTCACAGA AATCTGCAGG AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC
    GTGTCAGATT GGCTGGTTTC CATCGGTCTG CCCATGTATG CCAGCGCCCT CTCTGAGGCG
    GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT CTGTCCCACA CTTGCCTTCA GGAGGCTGGC
    ATCACAGAGG AGAGACACAT AAGCAAGCTC GTGTCTGCAG CCAGACTCTT CAAACTGCCA
    CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06173
    Clone ID Related Accession (Same CDS sequence) XM_005211030.4 , XM_005199430.3 , XM_005211030.3
    Accession Version XM_005211030.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product SAM and SH3 domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104231.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211030.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCCGAAGC CGTCAAGAGA ACAGTCGGAG GATGAGACGG AGGAGTCGGT GAAGTTTAAG 
    AGGTTACACA AGCTGGTAAA CTCCACTCGC AGAGTGAGAA AGAAACTCAT CAGGGTGGAA
    GAAATGAAAA AGCCCAGCAC AGAAGGCGGG GAGGAACAGG TGTTTGAGAA TTCCCCGGTC
    CCAGATGAAA GGTCCGCCCT GTACTCCGGC GTGCACAAGA AGCCCTTTTT CTTTGATGCT
    TCTCCTGAGA AACCCCCAGA AGATGACTCA GACTCTCTCA CCACTGCTCC ATCGTCCAGT
    AGCCTGGACA CCTGGGGTGC CGGCCGCAAA TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC
    CGGGGCCTGA TCAAGCCCCC CAAGAAGATG GGGACGTTCT TCTCGTACCC GGAAGACGAC
    AAGGCCCAGA AGGTGTCCCG CTCCCTCACC GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC
    CTGAGCCACG GGAGAACCTG CAGTTTTGGA GGATTTGACT TGACAAATCG TTCTCTGCAC
    ATTGGCAGTA ATAATTCGGA CCCAATGGGT AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA
    ATCAAATCAC CCACTGCCTC CCGAATCTCT CTTGGAAAAA AGGTGAAATC CGTGAAAGAG
    ACAATGAGGA AGAGAATGTC TAAAAAATAT AGCAGCTCTG TCTCTGAGCA GGATTCAGGC
    CTTGATGGGA TGCCGGGCTC CCCTCCGTCC TCTCAGCCTG ACAGTGAACA CATGGACAAG
    CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC
    TCGATGAGTG GTCAGACAGT GAGCACCACC GATTCCTCGA CCAGCAATAG GGAGAGTGTC
    AAGTCGGAAG ATGGTGATGA CGAGGAGCCC CCGTACCGGG GCCCGTTCTG TGGGCGTGCC
    AGGGTGCACA CCGACTTCAC GCCCAGCCCC TACGACACAG ACTCCCTCAA GCTCAAGAAA
    GGAGACATCA TTGATATAAT CAGCAAACCC CCCATGGGCA CCTGGATGGG CCTGCTGAAC
    AACAAGGTGG GCACGTTCAA ATTCATCTAC GTGGATGTGC TCAACGAGGA AGAGGAGAAG
    CCCAAGCGCG CCACCAGGAG GAGGAGGAAA GGGAGACCCC CTCAGCCGAA GTCAGTGGAG
    GACCTCCTGG ATCGGATCAA CCTCAAAGAG CACATGCCCA CTTTCCTGTT CAATGGCTAT
    GAAGATCTGG ACACCTTTAA GCTCCTAGAG GAGGAGGACT TGGACGAGTT AAATATCAGG
    GACCCAGAGC ACAGAGCTGT TCTCTTGACA GCAGTGGAGT TGTTACAGGA ATATGACAGT
    AACAGCGACC AGTCCGGATC CCAGGAGAAG CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA
    TGCTCTCCAC GAGACTCAGG ATGCTATGAA AGCAGTGAGA ATTTGGAAAA CGGCAAGACT
    CGGAAAACCG GCCTCTCTGC CAAGTCATCA ACCGAGTCCA GCTTGAAGTC TTTCAACAGG
    AACCAGCTGG GCAACTACCC AACCTTGCCT TTGACGAAGT CAGTGGACGC TTTGAAGCAG
    GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC ACCCCTCTCG CCTCCAAGAG CTGTGATCCC
    CCGTGTGTGA CTGATCTGAA TAAAAACCGA AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC
    TGTGAGACCC TAGAGGGCCC ACAGACCGTG GAGACTTGGC CCCGGTCCCA CTCCCTGGAC
    AACCTTCAAG GAGAGCCTGA GAAAGATGTG CCTGGCGAGG TGACAGAACC CTCTCCTGAG
    ATTGTACCTG AAGTGCCACA AAAGACAGCC CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA
    CGAGATTCTG CTGTTGACAA TGCACTGCTA CTGACCCAAA GCAAGAGATT TTCTGAACCT
    CAGAAAACGA CTGCTAAGAA ACTGGATGGC CCAATGGGGC CCTTTTCACG GGGTGCATCC
    CCCCCTCCAT GTTTGCCCAA AACCTTTGAC ACCCAGCCTC CTGCCATTAA ACCCAGTTTA
    ACACGGACGC CTCTGGAGGG TCATAGGAAA GGACTCGATT TCGAAGGAAC ACATCACCCC
    CCGGGCACCA AAGAAGGCAT GGACGCTGAG CAGAGGGGCC CCGAGGTCAG AACGCAGGCC
    AAACCTCCTG TCCAGCCTCC GCCCGTTCCC GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC
    GGGCTGCATC CTGTCCCTCC TGGTCCCCCC GGCGTCCCCA GCCCCGACGC ACCCGGCCTG
    CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC AGTGACTGTC CCCTGGTGGT GGCGGTGGCC
    AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC CCACCAAGCA CCAGGCCACC ACCCTGGCTG
    TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG GAGCACGGCG TGCGGCTCGG GCCCGCGCTG
    GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG GACCTAGAAA TGCTCACTGA GAACAAGCTG
    CGGGCGGAAG GCATTGACCT CACCGAGGAG CCCTATTCTG ATAAGCACGG CCGCTGTGGA
    ATTCCTGAGG CCCTGGTGCA GAGATATGCA GAAGACTTAG ACCAGCCGGA AAGGGATGTC
    GCCACCAACA TGGACCAGAT CCGTGTCAAG CTGCTTCGGA AGCAGCACCG CATGGCGATC
    CCAAGCGGTG GACTCACAGA AATCTGCAGG AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC
    GTGTCAGATT GGCTGGTTTC CATCGGTCTG CCCATGTATG CCAGCGCCCT CTCTGAGGCG
    GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT CTGTCCCACA CTTGCCTTCA GGAGGCTGGC
    ATCACAGAGG AGAGACACAT AAGCAAGCTC GTGTCTGCAG CCAGACTCTT CAAACTGCCA
    CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211087.1
    CDS1262..4282
    Translation

    Target ORF information:

    RefSeq Version XM_005211030.3
    Organism Bos taurus(cattle)
    Definition Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211030.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCCGAAGC CGTCAAGAGA ACAGTCGGAG GATGAGACGG AGGAGTCGGT GAAGTTTAAG 
    AGGTTACACA AGCTGGTAAA CTCCACTCGC AGAGTGAGAA AGAAACTCAT CAGGGTGGAA
    GAAATGAAAA AGCCCAGCAC AGAAGGCGGG GAGGAACAGG TGTTTGAGAA TTCCCCGGTC
    CCAGATGAAA GGTCCGCCCT GTACTCCGGC GTGCACAAGA AGCCCTTTTT CTTTGATGCT
    TCTCCTGAGA AACCCCCAGA AGATGACTCA GACTCTCTCA CCACTGCTCC ATCGTCCAGT
    AGCCTGGACA CCTGGGGTGC CGGCCGCAAA TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC
    CGGGGCCTGA TCAAGCCCCC CAAGAAGATG GGGACGTTCT TCTCGTACCC GGAAGACGAC
    AAGGCCCAGA AGGTGTCCCG CTCCCTCACC GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC
    CTGAGCCACG GGAGAACCTG CAGTTTTGGA GGATTTGACT TGACAAATCG TTCTCTGCAC
    ATTGGCAGTA ATAATTCGGA CCCAATGGGT AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA
    ATCAAATCAC CCACTGCCTC CCGAATCTCT CTTGGAAAAA AGGTGAAATC CGTGAAAGAG
    ACAATGAGGA AGAGAATGTC TAAAAAATAT AGCAGCTCTG TCTCTGAGCA GGATTCAGGC
    CTTGATGGGA TGCCGGGCTC CCCTCCGTCC TCTCAGCCTG ACAGTGAACA CATGGACAAG
    CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC
    TCGATGAGTG GTCAGACAGT GAGCACCACC GATTCCTCGA CCAGCAATAG GGAGAGTGTC
    AAGTCGGAAG ATGGTGATGA CGAGGAGCCC CCGTACCGGG GCCCGTTCTG TGGGCGTGCC
    AGGGTGCACA CCGACTTCAC GCCCAGCCCC TACGACACAG ACTCCCTCAA GCTCAAGAAA
    GGAGACATCA TTGATATAAT CAGCAAACCC CCCATGGGCA CCTGGATGGG CCTGCTGAAC
    AACAAGGTGG GCACGTTCAA ATTCATCTAC GTGGATGTGC TCAACGAGGA AGAGGAGAAG
    CCCAAGCGCG CCACCAGGAG GAGGAGGAAA GGGAGACCCC CTCAGCCGAA GTCAGTGGAG
    GACCTCCTGG ATCGGATCAA CCTCAAAGAG CACATGCCCA CTTTCCTGTT CAATGGCTAT
    GAAGATCTGG ACACCTTTAA GCTCCTAGAG GAGGAGGACT TGGACGAGTT AAATATCAGG
    GACCCAGAGC ACAGAGCTGT TCTCTTGACA GCAGTGGAGT TGTTACAGGA ATATGACAGT
    AACAGCGACC AGTCCGGATC CCAGGAGAAG CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA
    TGCTCTCCAC GAGACTCAGG ATGCTATGAA AGCAGTGAGA ATTTGGAAAA CGGCAAGACT
    CGGAAAACCG GCCTCTCTGC CAAGTCATCA ACCGAGTCCA GCTTGAAGTC TTTCAACAGG
    AACCAGCTGG GCAACTACCC AACCTTGCCT TTGACGAAGT CAGTGGACGC TTTGAAGCAG
    GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC ACCCCTCTCG CCTCCAAGAG CTGTGATCCC
    CCGTGTGTGA CTGATCTGAA TAAAAACCGA AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC
    TGTGAGACCC TAGAGGGCCC ACAGACCGTG GAGACTTGGC CCCGGTCCCA CTCCCTGGAC
    AACCTTCAAG GAGAGCCTGA GAAAGATGTG CCTGGCGAGG TGACAGAACC CTCTCCTGAG
    ATTGTACCTG AAGTGCCACA AAAGACAGCC CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA
    CGAGATTCTG CTGTTGACAA TGCACTGCTA CTGACCCAAA GCAAGAGATT TTCTGAACCT
    CAGAAAACGA CTGCTAAGAA ACTGGATGGC CCAATGGGGC CCTTTTCACG GGGTGCATCC
    CCCCCTCCAT GTTTGCCCAA AACCTTTGAC ACCCAGCCTC CTGCCATTAA ACCCAGTTTA
    ACACGGACGC CTCTGGAGGG TCATAGGAAA GGACTCGATT TCGAAGGAAC ACATCACCCC
    CCGGGCACCA AAGAAGGCAT GGACGCTGAG CAGAGGGGCC CCGAGGTCAG AACGCAGGCC
    AAACCTCCTG TCCAGCCTCC GCCCGTTCCC GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC
    GGGCTGCATC CTGTCCCTCC TGGTCCCCCC GGCGTCCCCA GCCCCGACGC ACCCGGCCTG
    CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC AGTGACTGTC CCCTGGTGGT GGCGGTGGCC
    AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC CCACCAAGCA CCAGGCCACC ACCCTGGCTG
    TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG GAGCACGGCG TGCGGCTCGG GCCCGCGCTG
    GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG GACCTAGAAA TGCTCACTGA GAACAAGCTG
    CGGGCGGAAG GCATTGACCT CACCGAGGAG CCCTATTCTG ATAAGCACGG CCGCTGTGGA
    ATTCCTGAGG CCCTGGTGCA GAGATATGCA GAAGACTTAG ACCAGCCGGA AAGGGATGTC
    GCCACCAACA TGGACCAGAT CCGTGTCAAG CTGCTTCGGA AGCAGCACCG CATGGCGATC
    CCAAGCGGTG GACTCACAGA AATCTGCAGG AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC
    GTGTCAGATT GGCTGGTTTC CATCGGTCTG CCCATGTATG CCAGCGCCCT CTCTGAGGCG
    GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT CTGTCCCACA CTTGCCTTCA GGAGGCTGGC
    ATCACAGAGG AGAGACACAT AAGCAAGCTC GTGTCTGCAG CCAGACTCTT CAAACTGCCA
    CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06174
    Clone ID Related Accession (Same CDS sequence) XM_005211031.4 , XM_005199431.3 , XM_005211031.3
    Accession Version XM_005211031.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product SAM and SH3 domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211031.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCTAAAA AGTCAAGAGA ACAGTCGGAG GATGAGACGG AGGAGTCGGT GAAGTTTAAG 
    AGGTTACACA AGCTGGTAAA CTCCACTCGC AGAGTGAGAA AGAAACTCAT CAGGGTGGAA
    GAAATGAAAA AGCCCAGCAC AGAAGGCGGG GAGGAACAGG TGTTTGAGAA TTCCCCGGTC
    CCAGATGAAA GGTCCGCCCT GTACTCCGGC GTGCACAAGA AGCCCTTTTT CTTTGATGCT
    TCTCCTGAGA AACCCCCAGA AGATGACTCA GACTCTCTCA CCACTGCTCC ATCGTCCAGT
    AGCCTGGACA CCTGGGGTGC CGGCCGCAAA TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC
    CGGGGCCTGA TCAAGCCCCC CAAGAAGATG GGGACGTTCT TCTCGTACCC GGAAGACGAC
    AAGGCCCAGA AGGTGTCCCG CTCCCTCACC GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC
    CTGAGCCACG GGAGAACCTG CAGTTTTGGA GGATTTGACT TGACAAATCG TTCTCTGCAC
    ATTGGCAGTA ATAATTCGGA CCCAATGGGT AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA
    ATCAAATCAC CCACTGCCTC CCGAATCTCT CTTGGAAAAA AGGTGAAATC CGTGAAAGAG
    ACAATGAGGA AGAGAATGTC TAAAAAATAT AGCAGCTCTG TCTCTGAGCA GGATTCAGGC
    CTTGATGGGA TGCCGGGCTC CCCTCCGTCC TCTCAGCCTG ACAGTGAACA CATGGACAAG
    CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC
    TCGATGAGTG GTCAGACAGT GAGCACCACC GATTCCTCGA CCAGCAATAG GGAGAGTGTC
    AAGTCGGAAG ATGGTGATGA CGAGGAGCCC CCGTACCGGG GCCCGTTCTG TGGGCGTGCC
    AGGGTGCACA CCGACTTCAC GCCCAGCCCC TACGACACAG ACTCCCTCAA GCTCAAGAAA
    GGAGACATCA TTGATATAAT CAGCAAACCC CCCATGGGCA CCTGGATGGG CCTGCTGAAC
    AACAAGGTGG GCACGTTCAA ATTCATCTAC GTGGATGTGC TCAACGAGGA AGAGGAGAAG
    CCCAAGCGCG CCACCAGGAG GAGGAGGAAA GGGAGACCCC CTCAGCCGAA GTCAGTGGAG
    GACCTCCTGG ATCGGATCAA CCTCAAAGAG CACATGCCCA CTTTCCTGTT CAATGGCTAT
    GAAGATCTGG ACACCTTTAA GCTCCTAGAG GAGGAGGACT TGGACGAGTT AAATATCAGG
    GACCCAGAGC ACAGAGCTGT TCTCTTGACA GCAGTGGAGT TGTTACAGGA ATATGACAGT
    AACAGCGACC AGTCCGGATC CCAGGAGAAG CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA
    TGCTCTCCAC GAGACTCAGG ATGCTATGAA AGCAGTGAGA ATTTGGAAAA CGGCAAGACT
    CGGAAAACCG GCCTCTCTGC CAAGTCATCA ACCGAGTCCA GCTTGAAGTC TTTCAACAGG
    AACCAGCTGG GCAACTACCC AACCTTGCCT TTGACGAAGT CAGTGGACGC TTTGAAGCAG
    GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC ACCCCTCTCG CCTCCAAGAG CTGTGATCCC
    CCGTGTGTGA CTGATCTGAA TAAAAACCGA AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC
    TGTGAGACCC TAGAGGGCCC ACAGACCGTG GAGACTTGGC CCCGGTCCCA CTCCCTGGAC
    AACCTTCAAG GAGAGCCTGA GAAAGATGTG CCTGGCGAGG TGACAGAACC CTCTCCTGAG
    ATTGTACCTG AAGTGCCACA AAAGACAGCC CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA
    CGAGATTCTG CTGTTGACAA TGCACTGCTA CTGACCCAAA GCAAGAGATT TTCTGAACCT
    CAGAAAACGA CTGCTAAGAA ACTGGATGGC CCAATGGGGC CCTTTTCACG GGGTGCATCC
    CCCCCTCCAT GTTTGCCCAA AACCTTTGAC ACCCAGCCTC CTGCCATTAA ACCCAGTTTA
    ACACGGACGC CTCTGGAGGG TCATAGGAAA GGACTCGATT TCGAAGGAAC ACATCACCCC
    CCGGGCACCA AAGAAGGCAT GGACGCTGAG CAGAGGGGCC CCGAGGTCAG AACGCAGGCC
    AAACCTCCTG TCCAGCCTCC GCCCGTTCCC GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC
    GGGCTGCATC CTGTCCCTCC TGGTCCCCCC GGCGTCCCCA GCCCCGACGC ACCCGGCCTG
    CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC AGTGACTGTC CCCTGGTGGT GGCGGTGGCC
    AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC CCACCAAGCA CCAGGCCACC ACCCTGGCTG
    TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG GAGCACGGCG TGCGGCTCGG GCCCGCGCTG
    GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG GACCTAGAAA TGCTCACTGA GAACAAGCTG
    CGGGCGGAAG GCATTGACCT CACCGAGGAG CCCTATTCTG ATAAGCACGG CCGCTGTGGA
    ATTCCTGAGG CCCTGGTGCA GAGATATGCA GAAGACTTAG ACCAGCCGGA AAGGGATGTC
    GCCACCAACA TGGACCAGAT CCGTGTCAAG CTGCTTCGGA AGCAGCACCG CATGGCGATC
    CCAAGCGGTG GACTCACAGA AATCTGCAGG AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC
    GTGTCAGATT GGCTGGTTTC CATCGGTCTG CCCATGTATG CCAGCGCCCT CTCTGAGGCG
    GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT CTGTCCCACA CTTGCCTTCA GGAGGCTGGC
    ATCACAGAGG AGAGACACAT AAGCAAGCTC GTGTCTGCAG CCAGACTCTT CAAACTGCCA
    CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211088.1
    CDS330..3350
    Translation

    Target ORF information:

    RefSeq Version XM_005211031.4
    Organism Bos taurus(cattle)
    Definition Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211031.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCTAAAA AGTCAAGAGA ACAGTCGGAG GATGAGACGG AGGAGTCGGT GAAGTTTAAG 
    AGGTTACACA AGCTGGTAAA CTCCACTCGC AGAGTGAGAA AGAAACTCAT CAGGGTGGAA
    GAAATGAAAA AGCCCAGCAC AGAAGGCGGG GAGGAACAGG TGTTTGAGAA TTCCCCGGTC
    CCAGATGAAA GGTCCGCCCT GTACTCCGGC GTGCACAAGA AGCCCTTTTT CTTTGATGCT
    TCTCCTGAGA AACCCCCAGA AGATGACTCA GACTCTCTCA CCACTGCTCC ATCGTCCAGT
    AGCCTGGACA CCTGGGGTGC CGGCCGCAAA TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC
    CGGGGCCTGA TCAAGCCCCC CAAGAAGATG GGGACGTTCT TCTCGTACCC GGAAGACGAC
    AAGGCCCAGA AGGTGTCCCG CTCCCTCACC GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC
    CTGAGCCACG GGAGAACCTG CAGTTTTGGA GGATTTGACT TGACAAATCG TTCTCTGCAC
    ATTGGCAGTA ATAATTCGGA CCCAATGGGT AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA
    ATCAAATCAC CCACTGCCTC CCGAATCTCT CTTGGAAAAA AGGTGAAATC CGTGAAAGAG
    ACAATGAGGA AGAGAATGTC TAAAAAATAT AGCAGCTCTG TCTCTGAGCA GGATTCAGGC
    CTTGATGGGA TGCCGGGCTC CCCTCCGTCC TCTCAGCCTG ACAGTGAACA CATGGACAAG
    CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC
    TCGATGAGTG GTCAGACAGT GAGCACCACC GATTCCTCGA CCAGCAATAG GGAGAGTGTC
    AAGTCGGAAG ATGGTGATGA CGAGGAGCCC CCGTACCGGG GCCCGTTCTG TGGGCGTGCC
    AGGGTGCACA CCGACTTCAC GCCCAGCCCC TACGACACAG ACTCCCTCAA GCTCAAGAAA
    GGAGACATCA TTGATATAAT CAGCAAACCC CCCATGGGCA CCTGGATGGG CCTGCTGAAC
    AACAAGGTGG GCACGTTCAA ATTCATCTAC GTGGATGTGC TCAACGAGGA AGAGGAGAAG
    CCCAAGCGCG CCACCAGGAG GAGGAGGAAA GGGAGACCCC CTCAGCCGAA GTCAGTGGAG
    GACCTCCTGG ATCGGATCAA CCTCAAAGAG CACATGCCCA CTTTCCTGTT CAATGGCTAT
    GAAGATCTGG ACACCTTTAA GCTCCTAGAG GAGGAGGACT TGGACGAGTT AAATATCAGG
    GACCCAGAGC ACAGAGCTGT TCTCTTGACA GCAGTGGAGT TGTTACAGGA ATATGACAGT
    AACAGCGACC AGTCCGGATC CCAGGAGAAG CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA
    TGCTCTCCAC GAGACTCAGG ATGCTATGAA AGCAGTGAGA ATTTGGAAAA CGGCAAGACT
    CGGAAAACCG GCCTCTCTGC CAAGTCATCA ACCGAGTCCA GCTTGAAGTC TTTCAACAGG
    AACCAGCTGG GCAACTACCC AACCTTGCCT TTGACGAAGT CAGTGGACGC TTTGAAGCAG
    GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC ACCCCTCTCG CCTCCAAGAG CTGTGATCCC
    CCGTGTGTGA CTGATCTGAA TAAAAACCGA AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC
    TGTGAGACCC TAGAGGGCCC ACAGACCGTG GAGACTTGGC CCCGGTCCCA CTCCCTGGAC
    AACCTTCAAG GAGAGCCTGA GAAAGATGTG CCTGGCGAGG TGACAGAACC CTCTCCTGAG
    ATTGTACCTG AAGTGCCACA AAAGACAGCC CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA
    CGAGATTCTG CTGTTGACAA TGCACTGCTA CTGACCCAAA GCAAGAGATT TTCTGAACCT
    CAGAAAACGA CTGCTAAGAA ACTGGATGGC CCAATGGGGC CCTTTTCACG GGGTGCATCC
    CCCCCTCCAT GTTTGCCCAA AACCTTTGAC ACCCAGCCTC CTGCCATTAA ACCCAGTTTA
    ACACGGACGC CTCTGGAGGG TCATAGGAAA GGACTCGATT TCGAAGGAAC ACATCACCCC
    CCGGGCACCA AAGAAGGCAT GGACGCTGAG CAGAGGGGCC CCGAGGTCAG AACGCAGGCC
    AAACCTCCTG TCCAGCCTCC GCCCGTTCCC GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC
    GGGCTGCATC CTGTCCCTCC TGGTCCCCCC GGCGTCCCCA GCCCCGACGC ACCCGGCCTG
    CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC AGTGACTGTC CCCTGGTGGT GGCGGTGGCC
    AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC CCACCAAGCA CCAGGCCACC ACCCTGGCTG
    TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG GAGCACGGCG TGCGGCTCGG GCCCGCGCTG
    GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG GACCTAGAAA TGCTCACTGA GAACAAGCTG
    CGGGCGGAAG GCATTGACCT CACCGAGGAG CCCTATTCTG ATAAGCACGG CCGCTGTGGA
    ATTCCTGAGG CCCTGGTGCA GAGATATGCA GAAGACTTAG ACCAGCCGGA AAGGGATGTC
    GCCACCAACA TGGACCAGAT CCGTGTCAAG CTGCTTCGGA AGCAGCACCG CATGGCGATC
    CCAAGCGGTG GACTCACAGA AATCTGCAGG AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC
    GTGTCAGATT GGCTGGTTTC CATCGGTCTG CCCATGTATG CCAGCGCCCT CTCTGAGGCG
    GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT CTGTCCCACA CTTGCCTTCA GGAGGCTGGC
    ATCACAGAGG AGAGACACAT AAGCAAGCTC GTGTCTGCAG CCAGACTCTT CAAACTGCCA
    CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06174
    Clone ID Related Accession (Same CDS sequence) XM_005211031.4 , XM_005199431.3 , XM_005211031.3
    Accession Version XM_005199431.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product SAM and SH3 domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645735.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199431.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCTAAAA AGTCAAGAGA ACAGTCGGAG GATGAGACGG AGGAGTCGGT GAAGTTTAAG 
    AGGTTACACA AGCTGGTAAA CTCCACTCGC AGAGTGAGAA AGAAACTCAT CAGGGTGGAA
    GAAATGAAAA AGCCCAGCAC AGAAGGCGGG GAGGAACAGG TGTTTGAGAA TTCCCCGGTC
    CCAGATGAAA GGTCCGCCCT GTACTCCGGC GTGCACAAGA AGCCCTTTTT CTTTGATGCT
    TCTCCTGAGA AACCCCCAGA AGATGACTCA GACTCTCTCA CCACTGCTCC ATCGTCCAGT
    AGCCTGGACA CCTGGGGTGC CGGCCGCAAA TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC
    CGGGGCCTGA TCAAGCCCCC CAAGAAGATG GGGACGTTCT TCTCGTACCC GGAAGACGAC
    AAGGCCCAGA AGGTGTCCCG CTCCCTCACC GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC
    CTGAGCCACG GGAGAACCTG CAGTTTTGGA GGATTTGACT TGACAAATCG TTCTCTGCAC
    ATTGGCAGTA ATAATTCGGA CCCAATGGGT AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA
    ATCAAATCAC CCACTGCCTC CCGAATCTCT CTTGGAAAAA AGGTGAAATC CGTGAAAGAG
    ACAATGAGGA AGAGAATGTC TAAAAAATAT AGCAGCTCTG TCTCTGAGCA GGATTCAGGC
    CTTGATGGGA TGCCGGGCTC CCCTCCGTCC TCTCAGCCTG ACAGTGAACA CATGGACAAG
    CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC
    TCGATGAGTG GTCAGACAGT GAGCACCACC GATTCCTCGA CCAGCAATAG GGAGAGTGTC
    AAGTCGGAAG ATGGTGATGA CGAGGAGCCC CCGTACCGGG GCCCGTTCTG TGGGCGTGCC
    AGGGTGCACA CCGACTTCAC GCCCAGCCCC TACGACACAG ACTCCCTCAA GCTCAAGAAA
    GGAGACATCA TTGATATAAT CAGCAAACCC CCCATGGGCA CCTGGATGGG CCTGCTGAAC
    AACAAGGTGG GCACGTTCAA ATTCATCTAC GTGGATGTGC TCAACGAGGA AGAGGAGAAG
    CCCAAGCGCG CCACCAGGAG GAGGAGGAAA GGGAGACCCC CTCAGCCGAA GTCAGTGGAG
    GACCTCCTGG ATCGGATCAA CCTCAAAGAG CACATGCCCA CTTTCCTGTT CAATGGCTAT
    GAAGATCTGG ACACCTTTAA GCTCCTAGAG GAGGAGGACT TGGACGAGTT AAATATCAGG
    GACCCAGAGC ACAGAGCTGT TCTCTTGACA GCAGTGGAGT TGTTACAGGA ATATGACAGT
    AACAGCGACC AGTCCGGATC CCAGGAGAAG CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA
    TGCTCTCCAC GAGACTCAGG ATGCTATGAA AGCAGTGAGA ATTTGGAAAA CGGCAAGACT
    CGGAAAACCG GCCTCTCTGC CAAGTCATCA ACCGAGTCCA GCTTGAAGTC TTTCAACAGG
    AACCAGCTGG GCAACTACCC AACCTTGCCT TTGACGAAGT CAGTGGACGC TTTGAAGCAG
    GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC ACCCCTCTCG CCTCCAAGAG CTGTGATCCC
    CCGTGTGTGA CTGATCTGAA TAAAAACCGA AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC
    TGTGAGACCC TAGAGGGCCC ACAGACCGTG GAGACTTGGC CCCGGTCCCA CTCCCTGGAC
    AACCTTCAAG GAGAGCCTGA GAAAGATGTG CCTGGCGAGG TGACAGAACC CTCTCCTGAG
    ATTGTACCTG AAGTGCCACA AAAGACAGCC CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA
    CGAGATTCTG CTGTTGACAA TGCACTGCTA CTGACCCAAA GCAAGAGATT TTCTGAACCT
    CAGAAAACGA CTGCTAAGAA ACTGGATGGC CCAATGGGGC CCTTTTCACG GGGTGCATCC
    CCCCCTCCAT GTTTGCCCAA AACCTTTGAC ACCCAGCCTC CTGCCATTAA ACCCAGTTTA
    ACACGGACGC CTCTGGAGGG TCATAGGAAA GGACTCGATT TCGAAGGAAC ACATCACCCC
    CCGGGCACCA AAGAAGGCAT GGACGCTGAG CAGAGGGGCC CCGAGGTCAG AACGCAGGCC
    AAACCTCCTG TCCAGCCTCC GCCCGTTCCC GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC
    GGGCTGCATC CTGTCCCTCC TGGTCCCCCC GGCGTCCCCA GCCCCGACGC ACCCGGCCTG
    CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC AGTGACTGTC CCCTGGTGGT GGCGGTGGCC
    AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC CCACCAAGCA CCAGGCCACC ACCCTGGCTG
    TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG GAGCACGGCG TGCGGCTCGG GCCCGCGCTG
    GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG GACCTAGAAA TGCTCACTGA GAACAAGCTG
    CGGGCGGAAG GCATTGACCT CACCGAGGAG CCCTATTCTG ATAAGCACGG CCGCTGTGGA
    ATTCCTGAGG CCCTGGTGCA GAGATATGCA GAAGACTTAG ACCAGCCGGA AAGGGATGTC
    GCCACCAACA TGGACCAGAT CCGTGTCAAG CTGCTTCGGA AGCAGCACCG CATGGCGATC
    CCAAGCGGTG GACTCACAGA AATCTGCAGG AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC
    GTGTCAGATT GGCTGGTTTC CATCGGTCTG CCCATGTATG CCAGCGCCCT CTCTGAGGCG
    GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT CTGTCCCACA CTTGCCTTCA GGAGGCTGGC
    ATCACAGAGG AGAGACACAT AAGCAAGCTC GTGTCTGCAG CCAGACTCTT CAAACTGCCA
    CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199488.1
    CDS329..3349
    Translation

    Target ORF information:

    RefSeq Version XM_005199431.3
    Organism Bos taurus(cattle)
    Definition Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199431.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCTAAAA AGTCAAGAGA ACAGTCGGAG GATGAGACGG AGGAGTCGGT GAAGTTTAAG 
    AGGTTACACA AGCTGGTAAA CTCCACTCGC AGAGTGAGAA AGAAACTCAT CAGGGTGGAA
    GAAATGAAAA AGCCCAGCAC AGAAGGCGGG GAGGAACAGG TGTTTGAGAA TTCCCCGGTC
    CCAGATGAAA GGTCCGCCCT GTACTCCGGC GTGCACAAGA AGCCCTTTTT CTTTGATGCT
    TCTCCTGAGA AACCCCCAGA AGATGACTCA GACTCTCTCA CCACTGCTCC ATCGTCCAGT
    AGCCTGGACA CCTGGGGTGC CGGCCGCAAA TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC
    CGGGGCCTGA TCAAGCCCCC CAAGAAGATG GGGACGTTCT TCTCGTACCC GGAAGACGAC
    AAGGCCCAGA AGGTGTCCCG CTCCCTCACC GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC
    CTGAGCCACG GGAGAACCTG CAGTTTTGGA GGATTTGACT TGACAAATCG TTCTCTGCAC
    ATTGGCAGTA ATAATTCGGA CCCAATGGGT AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA
    ATCAAATCAC CCACTGCCTC CCGAATCTCT CTTGGAAAAA AGGTGAAATC CGTGAAAGAG
    ACAATGAGGA AGAGAATGTC TAAAAAATAT AGCAGCTCTG TCTCTGAGCA GGATTCAGGC
    CTTGATGGGA TGCCGGGCTC CCCTCCGTCC TCTCAGCCTG ACAGTGAACA CATGGACAAG
    CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC
    TCGATGAGTG GTCAGACAGT GAGCACCACC GATTCCTCGA CCAGCAATAG GGAGAGTGTC
    AAGTCGGAAG ATGGTGATGA CGAGGAGCCC CCGTACCGGG GCCCGTTCTG TGGGCGTGCC
    AGGGTGCACA CCGACTTCAC GCCCAGCCCC TACGACACAG ACTCCCTCAA GCTCAAGAAA
    GGAGACATCA TTGATATAAT CAGCAAACCC CCCATGGGCA CCTGGATGGG CCTGCTGAAC
    AACAAGGTGG GCACGTTCAA ATTCATCTAC GTGGATGTGC TCAACGAGGA AGAGGAGAAG
    CCCAAGCGCG CCACCAGGAG GAGGAGGAAA GGGAGACCCC CTCAGCCGAA GTCAGTGGAG
    GACCTCCTGG ATCGGATCAA CCTCAAAGAG CACATGCCCA CTTTCCTGTT CAATGGCTAT
    GAAGATCTGG ACACCTTTAA GCTCCTAGAG GAGGAGGACT TGGACGAGTT AAATATCAGG
    GACCCAGAGC ACAGAGCTGT TCTCTTGACA GCAGTGGAGT TGTTACAGGA ATATGACAGT
    AACAGCGACC AGTCCGGATC CCAGGAGAAG CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA
    TGCTCTCCAC GAGACTCAGG ATGCTATGAA AGCAGTGAGA ATTTGGAAAA CGGCAAGACT
    CGGAAAACCG GCCTCTCTGC CAAGTCATCA ACCGAGTCCA GCTTGAAGTC TTTCAACAGG
    AACCAGCTGG GCAACTACCC AACCTTGCCT TTGACGAAGT CAGTGGACGC TTTGAAGCAG
    GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC ACCCCTCTCG CCTCCAAGAG CTGTGATCCC
    CCGTGTGTGA CTGATCTGAA TAAAAACCGA AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC
    TGTGAGACCC TAGAGGGCCC ACAGACCGTG GAGACTTGGC CCCGGTCCCA CTCCCTGGAC
    AACCTTCAAG GAGAGCCTGA GAAAGATGTG CCTGGCGAGG TGACAGAACC CTCTCCTGAG
    ATTGTACCTG AAGTGCCACA AAAGACAGCC CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA
    CGAGATTCTG CTGTTGACAA TGCACTGCTA CTGACCCAAA GCAAGAGATT TTCTGAACCT
    CAGAAAACGA CTGCTAAGAA ACTGGATGGC CCAATGGGGC CCTTTTCACG GGGTGCATCC
    CCCCCTCCAT GTTTGCCCAA AACCTTTGAC ACCCAGCCTC CTGCCATTAA ACCCAGTTTA
    ACACGGACGC CTCTGGAGGG TCATAGGAAA GGACTCGATT TCGAAGGAAC ACATCACCCC
    CCGGGCACCA AAGAAGGCAT GGACGCTGAG CAGAGGGGCC CCGAGGTCAG AACGCAGGCC
    AAACCTCCTG TCCAGCCTCC GCCCGTTCCC GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC
    GGGCTGCATC CTGTCCCTCC TGGTCCCCCC GGCGTCCCCA GCCCCGACGC ACCCGGCCTG
    CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC AGTGACTGTC CCCTGGTGGT GGCGGTGGCC
    AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC CCACCAAGCA CCAGGCCACC ACCCTGGCTG
    TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG GAGCACGGCG TGCGGCTCGG GCCCGCGCTG
    GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG GACCTAGAAA TGCTCACTGA GAACAAGCTG
    CGGGCGGAAG GCATTGACCT CACCGAGGAG CCCTATTCTG ATAAGCACGG CCGCTGTGGA
    ATTCCTGAGG CCCTGGTGCA GAGATATGCA GAAGACTTAG ACCAGCCGGA AAGGGATGTC
    GCCACCAACA TGGACCAGAT CCGTGTCAAG CTGCTTCGGA AGCAGCACCG CATGGCGATC
    CCAAGCGGTG GACTCACAGA AATCTGCAGG AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC
    GTGTCAGATT GGCTGGTTTC CATCGGTCTG CCCATGTATG CCAGCGCCCT CTCTGAGGCG
    GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT CTGTCCCACA CTTGCCTTCA GGAGGCTGGC
    ATCACAGAGG AGAGACACAT AAGCAAGCTC GTGTCTGCAG CCAGACTCTT CAAACTGCCA
    CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06174
    Clone ID Related Accession (Same CDS sequence) XM_005211031.4 , XM_005199431.3 , XM_005211031.3
    Accession Version XM_005211031.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product SAM and SH3 domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104231.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211031.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCTAAAA AGTCAAGAGA ACAGTCGGAG GATGAGACGG AGGAGTCGGT GAAGTTTAAG 
    AGGTTACACA AGCTGGTAAA CTCCACTCGC AGAGTGAGAA AGAAACTCAT CAGGGTGGAA
    GAAATGAAAA AGCCCAGCAC AGAAGGCGGG GAGGAACAGG TGTTTGAGAA TTCCCCGGTC
    CCAGATGAAA GGTCCGCCCT GTACTCCGGC GTGCACAAGA AGCCCTTTTT CTTTGATGCT
    TCTCCTGAGA AACCCCCAGA AGATGACTCA GACTCTCTCA CCACTGCTCC ATCGTCCAGT
    AGCCTGGACA CCTGGGGTGC CGGCCGCAAA TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC
    CGGGGCCTGA TCAAGCCCCC CAAGAAGATG GGGACGTTCT TCTCGTACCC GGAAGACGAC
    AAGGCCCAGA AGGTGTCCCG CTCCCTCACC GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC
    CTGAGCCACG GGAGAACCTG CAGTTTTGGA GGATTTGACT TGACAAATCG TTCTCTGCAC
    ATTGGCAGTA ATAATTCGGA CCCAATGGGT AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA
    ATCAAATCAC CCACTGCCTC CCGAATCTCT CTTGGAAAAA AGGTGAAATC CGTGAAAGAG
    ACAATGAGGA AGAGAATGTC TAAAAAATAT AGCAGCTCTG TCTCTGAGCA GGATTCAGGC
    CTTGATGGGA TGCCGGGCTC CCCTCCGTCC TCTCAGCCTG ACAGTGAACA CATGGACAAG
    CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC
    TCGATGAGTG GTCAGACAGT GAGCACCACC GATTCCTCGA CCAGCAATAG GGAGAGTGTC
    AAGTCGGAAG ATGGTGATGA CGAGGAGCCC CCGTACCGGG GCCCGTTCTG TGGGCGTGCC
    AGGGTGCACA CCGACTTCAC GCCCAGCCCC TACGACACAG ACTCCCTCAA GCTCAAGAAA
    GGAGACATCA TTGATATAAT CAGCAAACCC CCCATGGGCA CCTGGATGGG CCTGCTGAAC
    AACAAGGTGG GCACGTTCAA ATTCATCTAC GTGGATGTGC TCAACGAGGA AGAGGAGAAG
    CCCAAGCGCG CCACCAGGAG GAGGAGGAAA GGGAGACCCC CTCAGCCGAA GTCAGTGGAG
    GACCTCCTGG ATCGGATCAA CCTCAAAGAG CACATGCCCA CTTTCCTGTT CAATGGCTAT
    GAAGATCTGG ACACCTTTAA GCTCCTAGAG GAGGAGGACT TGGACGAGTT AAATATCAGG
    GACCCAGAGC ACAGAGCTGT TCTCTTGACA GCAGTGGAGT TGTTACAGGA ATATGACAGT
    AACAGCGACC AGTCCGGATC CCAGGAGAAG CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA
    TGCTCTCCAC GAGACTCAGG ATGCTATGAA AGCAGTGAGA ATTTGGAAAA CGGCAAGACT
    CGGAAAACCG GCCTCTCTGC CAAGTCATCA ACCGAGTCCA GCTTGAAGTC TTTCAACAGG
    AACCAGCTGG GCAACTACCC AACCTTGCCT TTGACGAAGT CAGTGGACGC TTTGAAGCAG
    GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC ACCCCTCTCG CCTCCAAGAG CTGTGATCCC
    CCGTGTGTGA CTGATCTGAA TAAAAACCGA AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC
    TGTGAGACCC TAGAGGGCCC ACAGACCGTG GAGACTTGGC CCCGGTCCCA CTCCCTGGAC
    AACCTTCAAG GAGAGCCTGA GAAAGATGTG CCTGGCGAGG TGACAGAACC CTCTCCTGAG
    ATTGTACCTG AAGTGCCACA AAAGACAGCC CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA
    CGAGATTCTG CTGTTGACAA TGCACTGCTA CTGACCCAAA GCAAGAGATT TTCTGAACCT
    CAGAAAACGA CTGCTAAGAA ACTGGATGGC CCAATGGGGC CCTTTTCACG GGGTGCATCC
    CCCCCTCCAT GTTTGCCCAA AACCTTTGAC ACCCAGCCTC CTGCCATTAA ACCCAGTTTA
    ACACGGACGC CTCTGGAGGG TCATAGGAAA GGACTCGATT TCGAAGGAAC ACATCACCCC
    CCGGGCACCA AAGAAGGCAT GGACGCTGAG CAGAGGGGCC CCGAGGTCAG AACGCAGGCC
    AAACCTCCTG TCCAGCCTCC GCCCGTTCCC GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC
    GGGCTGCATC CTGTCCCTCC TGGTCCCCCC GGCGTCCCCA GCCCCGACGC ACCCGGCCTG
    CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC AGTGACTGTC CCCTGGTGGT GGCGGTGGCC
    AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC CCACCAAGCA CCAGGCCACC ACCCTGGCTG
    TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG GAGCACGGCG TGCGGCTCGG GCCCGCGCTG
    GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG GACCTAGAAA TGCTCACTGA GAACAAGCTG
    CGGGCGGAAG GCATTGACCT CACCGAGGAG CCCTATTCTG ATAAGCACGG CCGCTGTGGA
    ATTCCTGAGG CCCTGGTGCA GAGATATGCA GAAGACTTAG ACCAGCCGGA AAGGGATGTC
    GCCACCAACA TGGACCAGAT CCGTGTCAAG CTGCTTCGGA AGCAGCACCG CATGGCGATC
    CCAAGCGGTG GACTCACAGA AATCTGCAGG AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC
    GTGTCAGATT GGCTGGTTTC CATCGGTCTG CCCATGTATG CCAGCGCCCT CTCTGAGGCG
    GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT CTGTCCCACA CTTGCCTTCA GGAGGCTGGC
    ATCACAGAGG AGAGACACAT AAGCAAGCTC GTGTCTGCAG CCAGACTCTT CAAACTGCCA
    CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211088.1
    CDS329..3349
    Translation

    Target ORF information:

    RefSeq Version XM_005211031.3
    Organism Bos taurus(cattle)
    Definition Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211031.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCTAAAA AGTCAAGAGA ACAGTCGGAG GATGAGACGG AGGAGTCGGT GAAGTTTAAG 
    AGGTTACACA AGCTGGTAAA CTCCACTCGC AGAGTGAGAA AGAAACTCAT CAGGGTGGAA
    GAAATGAAAA AGCCCAGCAC AGAAGGCGGG GAGGAACAGG TGTTTGAGAA TTCCCCGGTC
    CCAGATGAAA GGTCCGCCCT GTACTCCGGC GTGCACAAGA AGCCCTTTTT CTTTGATGCT
    TCTCCTGAGA AACCCCCAGA AGATGACTCA GACTCTCTCA CCACTGCTCC ATCGTCCAGT
    AGCCTGGACA CCTGGGGTGC CGGCCGCAAA TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC
    CGGGGCCTGA TCAAGCCCCC CAAGAAGATG GGGACGTTCT TCTCGTACCC GGAAGACGAC
    AAGGCCCAGA AGGTGTCCCG CTCCCTCACC GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC
    CTGAGCCACG GGAGAACCTG CAGTTTTGGA GGATTTGACT TGACAAATCG TTCTCTGCAC
    ATTGGCAGTA ATAATTCGGA CCCAATGGGT AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA
    ATCAAATCAC CCACTGCCTC CCGAATCTCT CTTGGAAAAA AGGTGAAATC CGTGAAAGAG
    ACAATGAGGA AGAGAATGTC TAAAAAATAT AGCAGCTCTG TCTCTGAGCA GGATTCAGGC
    CTTGATGGGA TGCCGGGCTC CCCTCCGTCC TCTCAGCCTG ACAGTGAACA CATGGACAAG
    CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC
    TCGATGAGTG GTCAGACAGT GAGCACCACC GATTCCTCGA CCAGCAATAG GGAGAGTGTC
    AAGTCGGAAG ATGGTGATGA CGAGGAGCCC CCGTACCGGG GCCCGTTCTG TGGGCGTGCC
    AGGGTGCACA CCGACTTCAC GCCCAGCCCC TACGACACAG ACTCCCTCAA GCTCAAGAAA
    GGAGACATCA TTGATATAAT CAGCAAACCC CCCATGGGCA CCTGGATGGG CCTGCTGAAC
    AACAAGGTGG GCACGTTCAA ATTCATCTAC GTGGATGTGC TCAACGAGGA AGAGGAGAAG
    CCCAAGCGCG CCACCAGGAG GAGGAGGAAA GGGAGACCCC CTCAGCCGAA GTCAGTGGAG
    GACCTCCTGG ATCGGATCAA CCTCAAAGAG CACATGCCCA CTTTCCTGTT CAATGGCTAT
    GAAGATCTGG ACACCTTTAA GCTCCTAGAG GAGGAGGACT TGGACGAGTT AAATATCAGG
    GACCCAGAGC ACAGAGCTGT TCTCTTGACA GCAGTGGAGT TGTTACAGGA ATATGACAGT
    AACAGCGACC AGTCCGGATC CCAGGAGAAG CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA
    TGCTCTCCAC GAGACTCAGG ATGCTATGAA AGCAGTGAGA ATTTGGAAAA CGGCAAGACT
    CGGAAAACCG GCCTCTCTGC CAAGTCATCA ACCGAGTCCA GCTTGAAGTC TTTCAACAGG
    AACCAGCTGG GCAACTACCC AACCTTGCCT TTGACGAAGT CAGTGGACGC TTTGAAGCAG
    GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC ACCCCTCTCG CCTCCAAGAG CTGTGATCCC
    CCGTGTGTGA CTGATCTGAA TAAAAACCGA AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC
    TGTGAGACCC TAGAGGGCCC ACAGACCGTG GAGACTTGGC CCCGGTCCCA CTCCCTGGAC
    AACCTTCAAG GAGAGCCTGA GAAAGATGTG CCTGGCGAGG TGACAGAACC CTCTCCTGAG
    ATTGTACCTG AAGTGCCACA AAAGACAGCC CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA
    CGAGATTCTG CTGTTGACAA TGCACTGCTA CTGACCCAAA GCAAGAGATT TTCTGAACCT
    CAGAAAACGA CTGCTAAGAA ACTGGATGGC CCAATGGGGC CCTTTTCACG GGGTGCATCC
    CCCCCTCCAT GTTTGCCCAA AACCTTTGAC ACCCAGCCTC CTGCCATTAA ACCCAGTTTA
    ACACGGACGC CTCTGGAGGG TCATAGGAAA GGACTCGATT TCGAAGGAAC ACATCACCCC
    CCGGGCACCA AAGAAGGCAT GGACGCTGAG CAGAGGGGCC CCGAGGTCAG AACGCAGGCC
    AAACCTCCTG TCCAGCCTCC GCCCGTTCCC GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC
    GGGCTGCATC CTGTCCCTCC TGGTCCCCCC GGCGTCCCCA GCCCCGACGC ACCCGGCCTG
    CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC AGTGACTGTC CCCTGGTGGT GGCGGTGGCC
    AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC CCACCAAGCA CCAGGCCACC ACCCTGGCTG
    TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG GAGCACGGCG TGCGGCTCGG GCCCGCGCTG
    GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG GACCTAGAAA TGCTCACTGA GAACAAGCTG
    CGGGCGGAAG GCATTGACCT CACCGAGGAG CCCTATTCTG ATAAGCACGG CCGCTGTGGA
    ATTCCTGAGG CCCTGGTGCA GAGATATGCA GAAGACTTAG ACCAGCCGGA AAGGGATGTC
    GCCACCAACA TGGACCAGAT CCGTGTCAAG CTGCTTCGGA AGCAGCACCG CATGGCGATC
    CCAAGCGGTG GACTCACAGA AATCTGCAGG AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC
    GTGTCAGATT GGCTGGTTTC CATCGGTCTG CCCATGTATG CCAGCGCCCT CTCTGAGGCG
    GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT CTGTCCCACA CTTGCCTTCA GGAGGCTGGC
    ATCACAGAGG AGAGACACAT AAGCAAGCTC GTGTCTGCAG CCAGACTCTT CAAACTGCCA
    CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06172
    Clone ID Related Accession (Same CDS sequence) XM_002690315.4 , XM_588302.7
    Accession Version XM_002690315.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product SAM and SH3 domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104231.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002690315.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGCAAG ACGCGGGAGC CGCCGGCGCG GCGCCCGAGC CGGAACCCGA GCCCGCTTCG 
    GAGCCCGAGG TGGGCGCGGG CATGTCCGAG GCGTTCTCCC GGCTCTGGAC CGACGTGATG
    GGCATCCTGG ATGGCTCCCT GGGAAACATC GATGACCTGG CTCAGCAGTA TGCAGATTAC
    TACAATACCT GCTTCTCCGA CGTGTGTGAG AGGATGGAGG AGCTGCGGAA ACGGCGGGTT
    TCCCAGGACC TGGACGTGGA GAAACCCGAA GCCAGCCCCA CGTCACTTCA GCTGCGGTCT
    CAGATCGAAG AGTCGCTTGG CTTCTGTAGC GCTGTATCAA CACCAGAAGT GGAAAGAAAG
    AATCCTCTCC ATAAGTCAAA CTCGGAAGAC AGCTCTGTAG GAAAGGGAGA TTGGAAGAAG
    AAAAATAAGT ATTTCTGGCA GAACTTCCGA AAGAATCAGA AAGGAATAAT GAGACAGACT
    TCAAAAGGAG AAGATGTGGG TTACGTTGCA AGCGAGATAA CAATGAGTGA TGAGGAACGG
    ATTCAGTTGA TGATGATGGT CAAGGAAAAG ATGATCACCA TCGAAGAAGC GCTTGCTCGG
    CTCAAGGAGT ATGAGGCCCA GCACCGGCAG TCCACTGCCT TGGATCCTGC CAACTGGCCG
    GACGGTTCTT ACCCAACATT TGAAGGCTCA TCAAACTGCA ATTCAAGAGA ACAGTCGGAG
    GATGAGACGG AGGAGTCGGT GAAGTTTAAG AGGTTACACA AGCTGGTAAA CTCCACTCGC
    AGAGTGAGAA AGAAACTCAT CAGGGTGGAA GAAATGAAAA AGCCCAGCAC AGAAGGCGGG
    GAGGAACAGG TGTTTGAGAA TTCCCCGGTC CCAGATGAAA GGTCCGCCCT GTACTCCGGC
    GTGCACAAGA AGCCCTTTTT CTTTGATGCT TCTCCTGAGA AACCCCCAGA AGATGACTCA
    GACTCTCTCA CCACTGCTCC ATCGTCCAGT AGCCTGGACA CCTGGGGTGC CGGCCGCAAA
    TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC CGGGGCCTGA TCAAGCCCCC CAAGAAGATG
    GGGACGTTCT TCTCGTACCC GGAAGACGAC AAGGCCCAGA AGGTGTCCCG CTCCCTCACC
    GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC CTGAGCCACG GGAGAACCTG CAGTTTTGGA
    GGATTTGACT TGACAAATCG TTCTCTGCAC ATTGGCAGTA ATAATTCGGA CCCAATGGGT
    AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA ATCAAATCAC CCACTGCCTC CCGAATCTCT
    CTTGGAAAAA AGGTGAAATC CGTGAAAGAG ACAATGAGGA AGAGAATGTC TAAAAAATAT
    AGCAGCTCTG TCTCTGAGCA GGATTCAGGC CTTGATGGGA TGCCGGGCTC CCCTCCGTCC
    TCTCAGCCTG ACAGTGAACA CATGGACAAG CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA
    AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC TCGATGAGTG GTCAGACAGT GAGCACCACC
    GATTCCTCGA CCAGCAATAG GGAGAGTGTC AAGTCGGAAG ATGGTGATGA CGAGGAGCCC
    CCGTACCGGG GCCCGTTCTG TGGGCGTGCC AGGGTGCACA CCGACTTCAC GCCCAGCCCC
    TACGACACAG ACTCCCTCAA GCTCAAGAAA GGAGACATCA TTGATATAAT CAGCAAACCC
    CCCATGGGCA CCTGGATGGG CCTGCTGAAC AACAAGGTGG GCACGTTCAA ATTCATCTAC
    GTGGATGTGC TCAACGAGGA AGAGGAGAAG CCCAAGCGCG CCACCAGGAG GAGGAGGAAA
    GGGAGACCCC CTCAGCCGAA GTCAGTGGAG GACCTCCTGG ATCGGATCAA CCTCAAAGAG
    CACATGCCCA CTTTCCTGTT CAATGGCTAT GAAGATCTGG ACACCTTTAA GCTCCTAGAG
    GAGGAGGACT TGGACGAGTT AAATATCAGG GACCCAGAGC ACAGAGCTGT TCTCTTGACA
    GCAGTGGAGT TGTTACAGGA ATATGACAGT AACAGCGACC AGTCCGGATC CCAGGAGAAG
    CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA TGCTCTCCAC GAGACTCAGG ATGCTATGAA
    AGCAGTGAGA ATTTGGAAAA CGGCAAGACT CGGAAAACCG GCCTCTCTGC CAAGTCATCA
    ACCGAGTCCA GCTTGAAGTC TTTCAACAGG AACCAGCTGG GCAACTACCC AACCTTGCCT
    TTGACGAAGT CAGTGGACGC TTTGAAGCAG GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC
    ACCCCTCTCG CCTCCAAGAG CTGTGATCCC CCGTGTGTGA CTGATCTGAA TAAAAACCGA
    AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC TGTGAGACCC TAGAGGGCCC ACAGACCGTG
    GAGACTTGGC CCCGGTCCCA CTCCCTGGAC AACCTTCAAG GAGAGCCTGA GAAAGATGTG
    CCTGGCGAGG TGACAGAACC CTCTCCTGAG ATTGTACCTG AAGTGCCACA AAAGACAGCC
    CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA CGAGATTCTG CTGTTGACAA TGCACTGCTA
    CTGACCCAAA GCAAGAGATT TTCTGAACCT CAGAAAACGA CTGCTAAGAA ACTGGATGGC
    CCAATGGGGC CCTTTTCACG GGGTGCATCC CCCCCTCCAT GTTTGCCCAA AACCTTTGAC
    ACCCAGCCTC CTGCCATTAA ACCCAGTTTA ACACGGACGC CTCTGGAGGG TCATAGGAAA
    GGACTCGATT TCGAAGGAAC ACATCACCCC CCGGGCACCA AAGAAGGCAT GGACGCTGAG
    CAGAGGGGCC CCGAGGTCAG AACGCAGGCC AAACCTCCTG TCCAGCCTCC GCCCGTTCCC
    GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC GGGCTGCATC CTGTCCCTCC TGGTCCCCCC
    GGCGTCCCCA GCCCCGACGC ACCCGGCCTG CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC
    AGTGACTGTC CCCTGGTGGT GGCGGTGGCC AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC
    CCACCAAGCA CCAGGCCACC ACCCTGGCTG TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG
    GAGCACGGCG TGCGGCTCGG GCCCGCGCTG GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG
    GACCTAGAAA TGCTCACTGA GAACAAGCTG CGGGCGGAAG GCATTGACCT CACCGAGGAG
    CCCTATTCTG ATAAGCACGG CCGCTGTGGA ATTCCTGAGG CCCTGGTGCA GAGATATGCA
    GAAGACTTAG ACCAGCCGGA AAGGGATGTC GCCACCAACA TGGACCAGAT CCGTGTCAAG
    CTGCTTCGGA AGCAGCACCG CATGGCGATC CCAAGCGGTG GACTCACAGA AATCTGCAGG
    AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC GTGTCAGATT GGCTGGTTTC CATCGGTCTG
    CCCATGTATG CCAGCGCCCT CTCTGAGGCG GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT
    CTGTCCCACA CTTGCCTTCA GGAGGCTGGC ATCACAGAGG AGAGACACAT AAGCAAGCTC
    GTGTCTGCAG CCAGACTCTT CAAACTGCCA CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002690361.1
    CDS655..4365
    Translation

    Target ORF information:

    RefSeq Version XM_002690315.4
    Organism Bos taurus(cattle)
    Definition Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002690315.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGCAAG ACGCGGGAGC CGCCGGCGCG GCGCCCGAGC CGGAACCCGA GCCCGCTTCG 
    GAGCCCGAGG TGGGCGCGGG CATGTCCGAG GCGTTCTCCC GGCTCTGGAC CGACGTGATG
    GGCATCCTGG ATGGCTCCCT GGGAAACATC GATGACCTGG CTCAGCAGTA TGCAGATTAC
    TACAATACCT GCTTCTCCGA CGTGTGTGAG AGGATGGAGG AGCTGCGGAA ACGGCGGGTT
    TCCCAGGACC TGGACGTGGA GAAACCCGAA GCCAGCCCCA CGTCACTTCA GCTGCGGTCT
    CAGATCGAAG AGTCGCTTGG CTTCTGTAGC GCTGTATCAA CACCAGAAGT GGAAAGAAAG
    AATCCTCTCC ATAAGTCAAA CTCGGAAGAC AGCTCTGTAG GAAAGGGAGA TTGGAAGAAG
    AAAAATAAGT ATTTCTGGCA GAACTTCCGA AAGAATCAGA AAGGAATAAT GAGACAGACT
    TCAAAAGGAG AAGATGTGGG TTACGTTGCA AGCGAGATAA CAATGAGTGA TGAGGAACGG
    ATTCAGTTGA TGATGATGGT CAAGGAAAAG ATGATCACCA TCGAAGAAGC GCTTGCTCGG
    CTCAAGGAGT ATGAGGCCCA GCACCGGCAG TCCACTGCCT TGGATCCTGC CAACTGGCCG
    GACGGTTCTT ACCCAACATT TGAAGGCTCA TCAAACTGCA ATTCAAGAGA ACAGTCGGAG
    GATGAGACGG AGGAGTCGGT GAAGTTTAAG AGGTTACACA AGCTGGTAAA CTCCACTCGC
    AGAGTGAGAA AGAAACTCAT CAGGGTGGAA GAAATGAAAA AGCCCAGCAC AGAAGGCGGG
    GAGGAACAGG TGTTTGAGAA TTCCCCGGTC CCAGATGAAA GGTCCGCCCT GTACTCCGGC
    GTGCACAAGA AGCCCTTTTT CTTTGATGCT TCTCCTGAGA AACCCCCAGA AGATGACTCA
    GACTCTCTCA CCACTGCTCC ATCGTCCAGT AGCCTGGACA CCTGGGGTGC CGGCCGCAAA
    TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC CGGGGCCTGA TCAAGCCCCC CAAGAAGATG
    GGGACGTTCT TCTCGTACCC GGAAGACGAC AAGGCCCAGA AGGTGTCCCG CTCCCTCACC
    GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC CTGAGCCACG GGAGAACCTG CAGTTTTGGA
    GGATTTGACT TGACAAATCG TTCTCTGCAC ATTGGCAGTA ATAATTCGGA CCCAATGGGT
    AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA ATCAAATCAC CCACTGCCTC CCGAATCTCT
    CTTGGAAAAA AGGTGAAATC CGTGAAAGAG ACAATGAGGA AGAGAATGTC TAAAAAATAT
    AGCAGCTCTG TCTCTGAGCA GGATTCAGGC CTTGATGGGA TGCCGGGCTC CCCTCCGTCC
    TCTCAGCCTG ACAGTGAACA CATGGACAAG CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA
    AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC TCGATGAGTG GTCAGACAGT GAGCACCACC
    GATTCCTCGA CCAGCAATAG GGAGAGTGTC AAGTCGGAAG ATGGTGATGA CGAGGAGCCC
    CCGTACCGGG GCCCGTTCTG TGGGCGTGCC AGGGTGCACA CCGACTTCAC GCCCAGCCCC
    TACGACACAG ACTCCCTCAA GCTCAAGAAA GGAGACATCA TTGATATAAT CAGCAAACCC
    CCCATGGGCA CCTGGATGGG CCTGCTGAAC AACAAGGTGG GCACGTTCAA ATTCATCTAC
    GTGGATGTGC TCAACGAGGA AGAGGAGAAG CCCAAGCGCG CCACCAGGAG GAGGAGGAAA
    GGGAGACCCC CTCAGCCGAA GTCAGTGGAG GACCTCCTGG ATCGGATCAA CCTCAAAGAG
    CACATGCCCA CTTTCCTGTT CAATGGCTAT GAAGATCTGG ACACCTTTAA GCTCCTAGAG
    GAGGAGGACT TGGACGAGTT AAATATCAGG GACCCAGAGC ACAGAGCTGT TCTCTTGACA
    GCAGTGGAGT TGTTACAGGA ATATGACAGT AACAGCGACC AGTCCGGATC CCAGGAGAAG
    CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA TGCTCTCCAC GAGACTCAGG ATGCTATGAA
    AGCAGTGAGA ATTTGGAAAA CGGCAAGACT CGGAAAACCG GCCTCTCTGC CAAGTCATCA
    ACCGAGTCCA GCTTGAAGTC TTTCAACAGG AACCAGCTGG GCAACTACCC AACCTTGCCT
    TTGACGAAGT CAGTGGACGC TTTGAAGCAG GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC
    ACCCCTCTCG CCTCCAAGAG CTGTGATCCC CCGTGTGTGA CTGATCTGAA TAAAAACCGA
    AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC TGTGAGACCC TAGAGGGCCC ACAGACCGTG
    GAGACTTGGC CCCGGTCCCA CTCCCTGGAC AACCTTCAAG GAGAGCCTGA GAAAGATGTG
    CCTGGCGAGG TGACAGAACC CTCTCCTGAG ATTGTACCTG AAGTGCCACA AAAGACAGCC
    CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA CGAGATTCTG CTGTTGACAA TGCACTGCTA
    CTGACCCAAA GCAAGAGATT TTCTGAACCT CAGAAAACGA CTGCTAAGAA ACTGGATGGC
    CCAATGGGGC CCTTTTCACG GGGTGCATCC CCCCCTCCAT GTTTGCCCAA AACCTTTGAC
    ACCCAGCCTC CTGCCATTAA ACCCAGTTTA ACACGGACGC CTCTGGAGGG TCATAGGAAA
    GGACTCGATT TCGAAGGAAC ACATCACCCC CCGGGCACCA AAGAAGGCAT GGACGCTGAG
    CAGAGGGGCC CCGAGGTCAG AACGCAGGCC AAACCTCCTG TCCAGCCTCC GCCCGTTCCC
    GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC GGGCTGCATC CTGTCCCTCC TGGTCCCCCC
    GGCGTCCCCA GCCCCGACGC ACCCGGCCTG CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC
    AGTGACTGTC CCCTGGTGGT GGCGGTGGCC AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC
    CCACCAAGCA CCAGGCCACC ACCCTGGCTG TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG
    GAGCACGGCG TGCGGCTCGG GCCCGCGCTG GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG
    GACCTAGAAA TGCTCACTGA GAACAAGCTG CGGGCGGAAG GCATTGACCT CACCGAGGAG
    CCCTATTCTG ATAAGCACGG CCGCTGTGGA ATTCCTGAGG CCCTGGTGCA GAGATATGCA
    GAAGACTTAG ACCAGCCGGA AAGGGATGTC GCCACCAACA TGGACCAGAT CCGTGTCAAG
    CTGCTTCGGA AGCAGCACCG CATGGCGATC CCAAGCGGTG GACTCACAGA AATCTGCAGG
    AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC GTGTCAGATT GGCTGGTTTC CATCGGTCTG
    CCCATGTATG CCAGCGCCCT CTCTGAGGCG GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT
    CTGTCCCACA CTTGCCTTCA GGAGGCTGGC ATCACAGAGG AGAGACACAT AAGCAAGCTC
    GTGTCTGCAG CCAGACTCTT CAAACTGCCA CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06172
    Clone ID Related Accession (Same CDS sequence) XM_002690315.4 , XM_588302.7
    Accession Version XM_588302.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product SAM and SH3 domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645735.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_588302.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGCAAG ACGCGGGAGC CGCCGGCGCG GCGCCCGAGC CGGAACCCGA GCCCGCTTCG 
    GAGCCCGAGG TGGGCGCGGG CATGTCCGAG GCGTTCTCCC GGCTCTGGAC CGACGTGATG
    GGCATCCTGG ATGGCTCCCT GGGAAACATC GATGACCTGG CTCAGCAGTA TGCAGATTAC
    TACAATACCT GCTTCTCCGA CGTGTGTGAG AGGATGGAGG AGCTGCGGAA ACGGCGGGTT
    TCCCAGGACC TGGACGTGGA GAAACCCGAA GCCAGCCCCA CGTCACTTCA GCTGCGGTCT
    CAGATCGAAG AGTCGCTTGG CTTCTGTAGC GCTGTATCAA CACCAGAAGT GGAAAGAAAG
    AATCCTCTCC ATAAGTCAAA CTCGGAAGAC AGCTCTGTAG GAAAGGGAGA TTGGAAGAAG
    AAAAATAAGT ATTTCTGGCA GAACTTCCGA AAGAATCAGA AAGGAATAAT GAGACAGACT
    TCAAAAGGAG AAGATGTGGG TTACGTTGCA AGCGAGATAA CAATGAGTGA TGAGGAACGG
    ATTCAGTTGA TGATGATGGT CAAGGAAAAG ATGATCACCA TCGAAGAAGC GCTTGCTCGG
    CTCAAGGAGT ATGAGGCCCA GCACCGGCAG TCCACTGCCT TGGATCCTGC CAACTGGCCG
    GACGGTTCTT ACCCAACATT TGAAGGCTCA TCAAACTGCA ATTCAAGAGA ACAGTCGGAG
    GATGAGACGG AGGAGTCGGT GAAGTTTAAG AGGTTACACA AGCTGGTAAA CTCCACTCGC
    AGAGTGAGAA AGAAACTCAT CAGGGTGGAA GAAATGAAAA AGCCCAGCAC AGAAGGCGGG
    GAGGAACAGG TGTTTGAGAA TTCCCCGGTC CCAGATGAAA GGTCCGCCCT GTACTCCGGC
    GTGCACAAGA AGCCCTTTTT CTTTGATGCT TCTCCTGAGA AACCCCCAGA AGATGACTCA
    GACTCTCTCA CCACTGCTCC ATCGTCCAGT AGCCTGGACA CCTGGGGTGC CGGCCGCAAA
    TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC CGGGGCCTGA TCAAGCCCCC CAAGAAGATG
    GGGACGTTCT TCTCGTACCC GGAAGACGAC AAGGCCCAGA AGGTGTCCCG CTCCCTCACC
    GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC CTGAGCCACG GGAGAACCTG CAGTTTTGGA
    GGATTTGACT TGACAAATCG TTCTCTGCAC ATTGGCAGTA ATAATTCGGA CCCAATGGGT
    AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA ATCAAATCAC CCACTGCCTC CCGAATCTCT
    CTTGGAAAAA AGGTGAAATC CGTGAAAGAG ACAATGAGGA AGAGAATGTC TAAAAAATAT
    AGCAGCTCTG TCTCTGAGCA GGATTCAGGC CTTGATGGGA TGCCGGGCTC CCCTCCGTCC
    TCTCAGCCTG ACAGTGAACA CATGGACAAG CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA
    AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC TCGATGAGTG GTCAGACAGT GAGCACCACC
    GATTCCTCGA CCAGCAATAG GGAGAGTGTC AAGTCGGAAG ATGGTGATGA CGAGGAGCCC
    CCGTACCGGG GCCCGTTCTG TGGGCGTGCC AGGGTGCACA CCGACTTCAC GCCCAGCCCC
    TACGACACAG ACTCCCTCAA GCTCAAGAAA GGAGACATCA TTGATATAAT CAGCAAACCC
    CCCATGGGCA CCTGGATGGG CCTGCTGAAC AACAAGGTGG GCACGTTCAA ATTCATCTAC
    GTGGATGTGC TCAACGAGGA AGAGGAGAAG CCCAAGCGCG CCACCAGGAG GAGGAGGAAA
    GGGAGACCCC CTCAGCCGAA GTCAGTGGAG GACCTCCTGG ATCGGATCAA CCTCAAAGAG
    CACATGCCCA CTTTCCTGTT CAATGGCTAT GAAGATCTGG ACACCTTTAA GCTCCTAGAG
    GAGGAGGACT TGGACGAGTT AAATATCAGG GACCCAGAGC ACAGAGCTGT TCTCTTGACA
    GCAGTGGAGT TGTTACAGGA ATATGACAGT AACAGCGACC AGTCCGGATC CCAGGAGAAG
    CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA TGCTCTCCAC GAGACTCAGG ATGCTATGAA
    AGCAGTGAGA ATTTGGAAAA CGGCAAGACT CGGAAAACCG GCCTCTCTGC CAAGTCATCA
    ACCGAGTCCA GCTTGAAGTC TTTCAACAGG AACCAGCTGG GCAACTACCC AACCTTGCCT
    TTGACGAAGT CAGTGGACGC TTTGAAGCAG GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC
    ACCCCTCTCG CCTCCAAGAG CTGTGATCCC CCGTGTGTGA CTGATCTGAA TAAAAACCGA
    AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC TGTGAGACCC TAGAGGGCCC ACAGACCGTG
    GAGACTTGGC CCCGGTCCCA CTCCCTGGAC AACCTTCAAG GAGAGCCTGA GAAAGATGTG
    CCTGGCGAGG TGACAGAACC CTCTCCTGAG ATTGTACCTG AAGTGCCACA AAAGACAGCC
    CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA CGAGATTCTG CTGTTGACAA TGCACTGCTA
    CTGACCCAAA GCAAGAGATT TTCTGAACCT CAGAAAACGA CTGCTAAGAA ACTGGATGGC
    CCAATGGGGC CCTTTTCACG GGGTGCATCC CCCCCTCCAT GTTTGCCCAA AACCTTTGAC
    ACCCAGCCTC CTGCCATTAA ACCCAGTTTA ACACGGACGC CTCTGGAGGG TCATAGGAAA
    GGACTCGATT TCGAAGGAAC ACATCACCCC CCGGGCACCA AAGAAGGCAT GGACGCTGAG
    CAGAGGGGCC CCGAGGTCAG AACGCAGGCC AAACCTCCTG TCCAGCCTCC GCCCGTTCCC
    GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC GGGCTGCATC CTGTCCCTCC TGGTCCCCCC
    GGCGTCCCCA GCCCCGACGC ACCCGGCCTG CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC
    AGTGACTGTC CCCTGGTGGT GGCGGTGGCC AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC
    CCACCAAGCA CCAGGCCACC ACCCTGGCTG TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG
    GAGCACGGCG TGCGGCTCGG GCCCGCGCTG GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG
    GACCTAGAAA TGCTCACTGA GAACAAGCTG CGGGCGGAAG GCATTGACCT CACCGAGGAG
    CCCTATTCTG ATAAGCACGG CCGCTGTGGA ATTCCTGAGG CCCTGGTGCA GAGATATGCA
    GAAGACTTAG ACCAGCCGGA AAGGGATGTC GCCACCAACA TGGACCAGAT CCGTGTCAAG
    CTGCTTCGGA AGCAGCACCG CATGGCGATC CCAAGCGGTG GACTCACAGA AATCTGCAGG
    AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC GTGTCAGATT GGCTGGTTTC CATCGGTCTG
    CCCATGTATG CCAGCGCCCT CTCTGAGGCG GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT
    CTGTCCCACA CTTGCCTTCA GGAGGCTGGC ATCACAGAGG AGAGACACAT AAGCAAGCTC
    GTGTCTGCAG CCAGACTCTT CAAACTGCCA CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_588302.4
    CDS655..4365
    Translation

    Target ORF information:

    RefSeq Version XM_588302.7
    Organism Bos taurus(cattle)
    Definition Bos taurus SAM and SH3 domain containing 1 (SASH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_588302.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGCAAG ACGCGGGAGC CGCCGGCGCG GCGCCCGAGC CGGAACCCGA GCCCGCTTCG 
    GAGCCCGAGG TGGGCGCGGG CATGTCCGAG GCGTTCTCCC GGCTCTGGAC CGACGTGATG
    GGCATCCTGG ATGGCTCCCT GGGAAACATC GATGACCTGG CTCAGCAGTA TGCAGATTAC
    TACAATACCT GCTTCTCCGA CGTGTGTGAG AGGATGGAGG AGCTGCGGAA ACGGCGGGTT
    TCCCAGGACC TGGACGTGGA GAAACCCGAA GCCAGCCCCA CGTCACTTCA GCTGCGGTCT
    CAGATCGAAG AGTCGCTTGG CTTCTGTAGC GCTGTATCAA CACCAGAAGT GGAAAGAAAG
    AATCCTCTCC ATAAGTCAAA CTCGGAAGAC AGCTCTGTAG GAAAGGGAGA TTGGAAGAAG
    AAAAATAAGT ATTTCTGGCA GAACTTCCGA AAGAATCAGA AAGGAATAAT GAGACAGACT
    TCAAAAGGAG AAGATGTGGG TTACGTTGCA AGCGAGATAA CAATGAGTGA TGAGGAACGG
    ATTCAGTTGA TGATGATGGT CAAGGAAAAG ATGATCACCA TCGAAGAAGC GCTTGCTCGG
    CTCAAGGAGT ATGAGGCCCA GCACCGGCAG TCCACTGCCT TGGATCCTGC CAACTGGCCG
    GACGGTTCTT ACCCAACATT TGAAGGCTCA TCAAACTGCA ATTCAAGAGA ACAGTCGGAG
    GATGAGACGG AGGAGTCGGT GAAGTTTAAG AGGTTACACA AGCTGGTAAA CTCCACTCGC
    AGAGTGAGAA AGAAACTCAT CAGGGTGGAA GAAATGAAAA AGCCCAGCAC AGAAGGCGGG
    GAGGAACAGG TGTTTGAGAA TTCCCCGGTC CCAGATGAAA GGTCCGCCCT GTACTCCGGC
    GTGCACAAGA AGCCCTTTTT CTTTGATGCT TCTCCTGAGA AACCCCCAGA AGATGACTCA
    GACTCTCTCA CCACTGCTCC ATCGTCCAGT AGCCTGGACA CCTGGGGTGC CGGCCGCAAA
    TTGGTCAAAA CCTTCAGCAA AGGAGAGAGC CGGGGCCTGA TCAAGCCCCC CAAGAAGATG
    GGGACGTTCT TCTCGTACCC GGAAGACGAC AAGGCCCAGA AGGTGTCCCG CTCCCTCACC
    GAAGGGGAGA TGAAGAAGGG GCTTGGGTCC CTGAGCCACG GGAGAACCTG CAGTTTTGGA
    GGATTTGACT TGACAAATCG TTCTCTGCAC ATTGGCAGTA ATAATTCGGA CCCAATGGGT
    AAAGAAGGAG ATTTTGTGTA TAAAGAAGTA ATCAAATCAC CCACTGCCTC CCGAATCTCT
    CTTGGAAAAA AGGTGAAATC CGTGAAAGAG ACAATGAGGA AGAGAATGTC TAAAAAATAT
    AGCAGCTCTG TCTCTGAGCA GGATTCAGGC CTTGATGGGA TGCCGGGCTC CCCTCCGTCC
    TCTCAGCCTG ACAGTGAACA CATGGACAAG CCCAAGCTCA AAGCCGGAGG TTCTGTGGAA
    AGTCTGCGGA GTTCTCTGAG CGGTCAAAGC TCGATGAGTG GTCAGACAGT GAGCACCACC
    GATTCCTCGA CCAGCAATAG GGAGAGTGTC AAGTCGGAAG ATGGTGATGA CGAGGAGCCC
    CCGTACCGGG GCCCGTTCTG TGGGCGTGCC AGGGTGCACA CCGACTTCAC GCCCAGCCCC
    TACGACACAG ACTCCCTCAA GCTCAAGAAA GGAGACATCA TTGATATAAT CAGCAAACCC
    CCCATGGGCA CCTGGATGGG CCTGCTGAAC AACAAGGTGG GCACGTTCAA ATTCATCTAC
    GTGGATGTGC TCAACGAGGA AGAGGAGAAG CCCAAGCGCG CCACCAGGAG GAGGAGGAAA
    GGGAGACCCC CTCAGCCGAA GTCAGTGGAG GACCTCCTGG ATCGGATCAA CCTCAAAGAG
    CACATGCCCA CTTTCCTGTT CAATGGCTAT GAAGATCTGG ACACCTTTAA GCTCCTAGAG
    GAGGAGGACT TGGACGAGTT AAATATCAGG GACCCAGAGC ACAGAGCTGT TCTCTTGACA
    GCAGTGGAGT TGTTACAGGA ATATGACAGT AACAGCGACC AGTCCGGATC CCAGGAGAAG
    CTGCTTGTTG ACAGCCAGGG CTTGAGCGGA TGCTCTCCAC GAGACTCAGG ATGCTATGAA
    AGCAGTGAGA ATTTGGAAAA CGGCAAGACT CGGAAAACCG GCCTCTCTGC CAAGTCATCA
    ACCGAGTCCA GCTTGAAGTC TTTCAACAGG AACCAGCTGG GCAACTACCC AACCTTGCCT
    TTGACGAAGT CAGTGGACGC TTTGAAGCAG GGAGGGGAGG GGAGGCTGGG CGGTGGTCTC
    ACCCCTCTCG CCTCCAAGAG CTGTGATCCC CCGTGTGTGA CTGATCTGAA TAAAAACCGA
    AGGAGTCTCC CCGTTTCCAT CTGCCGGAGC TGTGAGACCC TAGAGGGCCC ACAGACCGTG
    GAGACTTGGC CCCGGTCCCA CTCCCTGGAC AACCTTCAAG GAGAGCCTGA GAAAGATGTG
    CCTGGCGAGG TGACAGAACC CTCTCCTGAG ATTGTACCTG AAGTGCCACA AAAGACAGCC
    CCCTCCGTCA CAAAGGTTCC AAGCCCCAAA CGAGATTCTG CTGTTGACAA TGCACTGCTA
    CTGACCCAAA GCAAGAGATT TTCTGAACCT CAGAAAACGA CTGCTAAGAA ACTGGATGGC
    CCAATGGGGC CCTTTTCACG GGGTGCATCC CCCCCTCCAT GTTTGCCCAA AACCTTTGAC
    ACCCAGCCTC CTGCCATTAA ACCCAGTTTA ACACGGACGC CTCTGGAGGG TCATAGGAAA
    GGACTCGATT TCGAAGGAAC ACATCACCCC CCGGGCACCA AAGAAGGCAT GGACGCTGAG
    CAGAGGGGCC CCGAGGTCAG AACGCAGGCC AAACCTCCTG TCCAGCCTCC GCCCGTTCCC
    GCCAAGAAGA GCAGGGAGCG CCTCGCCAAC GGGCTGCATC CTGTCCCTCC TGGTCCCCCC
    GGCGTCCCCA GCCCCGACGC ACCCGGCCTG CCGCTTAAAA AGGGCAGCCC TGGCGGCCCC
    AGTGACTGTC CCCTGGTGGT GGCGGTGGCC AGGCCTCCCC CGGGGCCGGA GCCAGGCAGC
    CCACCAAGCA CCAGGCCACC ACCCTGGCTG TCGGAGCTGC CCGAGAGCGC CAGCCTCCAG
    GAGCACGGCG TGCGGCTCGG GCCCGCGCTG GCCAGGAAGG TCTCCTGCGC CCGCGGGCTG
    GACCTAGAAA TGCTCACTGA GAACAAGCTG CGGGCGGAAG GCATTGACCT CACCGAGGAG
    CCCTATTCTG ATAAGCACGG CCGCTGTGGA ATTCCTGAGG CCCTGGTGCA GAGATATGCA
    GAAGACTTAG ACCAGCCGGA AAGGGATGTC GCCACCAACA TGGACCAGAT CCGTGTCAAG
    CTGCTTCGGA AGCAGCACCG CATGGCGATC CCAAGCGGTG GACTCACAGA AATCTGCAGG
    AAGCCCCTCT CTCCCGGCTA CGTGTCGTCC GTGTCAGATT GGCTGGTTTC CATCGGTCTG
    CCCATGTATG CCAGCGCCCT CTCTGAGGCG GGCTTTAGCA CCCTGGGCCA AGTGCCTTCT
    CTGTCCCACA CTTGCCTTCA GGAGGCTGGC ATCACAGAGG AGAGACACAT AAGCAAGCTC
    GTGTCTGCAG CCAGACTCTT CAAACTGCCA CCAGGCCCGG AGGCCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.