SASH1 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_004935597.2	XP_004935654.1	SAM and SH3 domain-containing protein 1 isoform X2
XM_025149371.1	XP_025005139.1	SAM and SH3 domain-containing protein 1 isoform X3
XM_025149373.1	XP_025005141.1	SAM and SH3 domain-containing protein 1 isoform X5
XM_025149374.1	XP_025005142.1	SAM and SH3 domain-containing protein 1 isoform X6
XM_004935596.2	XP_004935653.1	SAM and SH3 domain-containing protein 1 isoform X1
XM_004935598.2	XP_004935655.1	SAM and SH3 domain-containing protein 1 isoform X3
XM_025149369.1	XP_025005137.1	SAM and SH3 domain-containing protein 1 isoform X1
XM_025149372.1	XP_025005140.1	SAM and SH3 domain-containing protein 1 isoform X4
XM_025149376.1	XP_025005144.1	SAM and SH3 domain-containing protein 1 isoform X9
XM_015284279.1	XP_015139765.1	SAM and SH3 domain-containing protein 1 isoform X7
XM_025149375.1	XP_025005143.1	SAM and SH3 domain-containing protein 1 isoform X7
XM_015284279.2	XP_015139765.1	SAM and SH3 domain-containing protein 1 isoform X8
XM_025149370.1	XP_025005138.1	SAM and SH3 domain-containing protein 1 isoform X2
XM_025149377.1	XP_025005145.1	SAM and SH3 domain-containing protein 1 isoform X10
XM_015284278.1	XP_015139764.1	SAM and SH3 domain-containing protein 1 isoform X4
XM_419659.5	XP_419659.5	SAM and SH3 domain-containing protein 1 isoform X5
XM_004935599.2	XP_004935656.1	SAM and SH3 domain-containing protein 1 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa149131	XM_025149369.1 Latest version!	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa149132	XM_025149370.1 Latest version!	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa149133	XM_025149371.1 Latest version!	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa149134	XM_025149373.1 Latest version!	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa149135	XM_025149374.1 Latest version!	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa149136	XM_025149375.1 Latest version!	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa149137	XM_025149376.1 Latest version!	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa149138	XM_025149377.1 Latest version!	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa33753	XM_015284278.1 Latest version!	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa33754	XM_419659.5 Latest version!	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa33755	XM_015284279.2 Latest version!	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa06284	XM_004935599.2 Latest version!	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa06281	XM_025149372.1 Latest version!	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OGa06281	XM_004935596.2 Latest version!	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OGa06282	XM_004935597.2 Latest version!	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OGa06283	XM_004935598.2 Latest version!	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
OGa33755	XM_015284279.1	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

SASH1 ( XM_025149369.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005137.1 Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa149131
Clone ID Related Accession (Same CDS sequence)	XM_025149369.1
Accession Version	XM_025149369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	SAM and SH3 domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACCA ACATAGTGTA CGAGTGGCTG AAAACCCTGC AGCTCCCCCA GTACGCCGAG 
TCCTTTGTCG ACAATGGCTA CGACGACCTG GAGGTCTGCA AGCAGATCGG CGACCCGGAC 
CTGGATGCCA TCGGCGTGGC CGTGCCCCAG CACCGCCGCC GCATCCACGA GGCCGTGCGG 
CGGCTGCGGG AGGCGGACGA GCGGGCGGCG GGGCTCTACT TCACCCTGGA GCCGCCGCCC 
GCGCCTCCCG CCAAGGGGCC CCGCCGCCCG CCGGGGCAGG ACGGGGCGGC CCCCCGGCAC 
CGGCCTGGCC GCGGGGGCCC GGTGGTCTAC CCCAGGCTGA AGCTGAAGAT CATGATCCGG 
GACAAGCTGG TCCGCGACGG CATCAACCTG AGCAAGCCCC CCTACTCCAA CAAGGATGGG 
TCTCTGGGAA ACATTGATGA CCTCGCACAG CAGTATGCAG ATTATTACAA CACCTGCTTC 
ACGGATGTGT GTGAGAGGAT GGAAGAGCTG CGCAAACGGA GGGTTTCCCA GGACCTTGAT 
GTGGAGAAAC CTGATGCCAG TCCCACATCT CTACAACTGC GATCCCAGAT TGAGGAGTCA 
CTTGGTCTCA GCAGCACCGC ATCAACACCT GACACTGAAA GAAAGCTCTC CATTCATAAA 
TCAAGTTCGG AAGAAGGCTC TGTAGGGAAA GCAGACTGGA AAAAGAAAAA TAAGTTTTTT 
TGGCAAAATT TCCGAAAGAA CCAGAAAGGA TTGATGAGGC AGACTTCAAA AGGAGAGGAT 
GTGGGGTATG TGGCCAGTGA GATTACGATG AGCGATGAGG AACGGATCCA GCTGATGATG 
ATGGTGAAAG AGAAGATGAT CACTATTGAG GAAGCACTTG CTAGGCTGAA GGAGTATGAG 
TCCCAGCACA GGCAATCAAG TACTGTAGAT GCTACAGACT GGCCTGATGG TTCTTACTCA 
ACATTTGATG GGTCTTCCAA TTGTAATTCA AGAGAACAAT CCGATGATGA AACAGAGGAG 
TCGGTGAAGT TTAAGAGATT GCACAAGCTG GTAAATTCTA CTCGCAGAGT CAGGAAGAAA 
CTCATAAGGG TGGAAGAAAT GAAAAAACCC AGTACAGAAG GCGTAGAGGA GCAGTCGCTT 
GACAACTCTG CTATACTGGA TGACAGATCG GCACTTTACT CTGGAGTTCA CAAGAAGCAA 
TTCTACTTTG ATGGTTCCTC CGAGAAGCAG ACAGAGGATG ACTCGGACTC ACTTACTACT 
TCTCCCTCAT CTAGTAGTCT TGATACATGG GGAGCTAGCC GGAAACTGGT GAAGACCTTC 
AGCAAAACTG ATAGCCGTGG CCTTATCAAG CCTCCCAAGA AACTTGGGAC ATTTTTTTCT 
TACCCAGAAG ATGAGAAGAC CCAGAAAGTT TGCCGCTCCT TGACAGATGG GGAGATGAAG 
AAGAGCCTCG GCTCACTGAG CCATGGGGTA AGTACAGAAA GCATTTGCTT TTATGACAGC 
AACAGGCACA AGCACCATCT TCTGGTGTCC CTGGAGGAGA TTCAGAGTGT AAGGAAGCAG 
ATCCGATTGA AGAAAATGGA TACTAACTAT TCCTGTTCCA GAGCTTTTCT TTGCCAGCGC 
CCTGCTAGGA AAGGAGCATC CGCCACAACT TGCGACCTAC AATTACTGCG GCACAAAGTG 
AAAGGGGGTG GAGGTTGTGG CTTCCCAAAC AGAAGGCTAC GAGGGCGCAC TTCTGTCAGC 
GAGTTCAATA TTACCTATGT GGTGGAGAGG AGCCTGTACA GTCACCTCAA CCTCTCACAG 
CTGGTGCGTC CCGTTACTGA CAGGACCCTG AGCAAAGCTG ACAAGCAGGA CCTGAGGAGA 
TGCCTGCTAG AGGAGGATGA GGAGGCCAAG AGGAAGTGGG CTGCCACAGT GGATCGTTGT 
ACTAAAAGGG TTCTCTTGAG AATCCACCAA AAGTCTAGAA CCTGCAGCTT TGGAGGATTT 
GACTTGACAA ATCGTTCCCT TCATATTGGA AACAATTCTG ACCAAATGGG CAAAGAAGGT 
GACTTTGTTT ATAAAGAAGT AATCAAATCA CCCTCTGCCT CCCGTATATC TCTGGGCAAA 
AAGGTGAAAT CTGTGAAAGA AACCATGAAG AAAAGGATGT CTAAAAAATA CAGCAGCTCT 
CTGTCTGAGC AGGAGTCGAG CCCTGGAGTC ATACCGGGTT CTCCGCAGTC GCCGCCGCCA 
GACACTGACT CTCTGGACAA GCCCAAGCTG AAAGCTGGTG GCTCAGTTGA GAGCCTGAGG 
AGTTCCTTAA GTGGTCAGAG CTCAATGAGT GGTCAGACAG TGAGTACAAC AGATTCCTCA 
ACCAGCAACA GGGAGAGCGT GAAATCAGAA GATGGGGATG ATGAAGAACC TCCCTACAGA 
GGACCCTTCT GTGGAAGAGC CAGGGTTCAC ACTGATTTTA CTCCAAGTCC TTATGATACA 
GACTCTCTGA AGCTCAAGAA AGGTGATATC ATTGACATCA TCAGCAAACC CCCCATGGGC 
ACTTGGATGG GCCTATTGAA CAACAAAGTT GGCACTTTCA AATTTATCTA TGTGGATGTG 
TTAAATGAAG AGGAAGAGAA GCCGAAGCGA CCCACCAGGA GACGCCGAAA AAGCAGACCA 
CCCCAACCCA AGTCAGTTGA GGATCTCTTG GATCGCATCA ACTTAAAGGA GCACATGCCC 
ACTTTTCTGT TCAATGGTTA TGAAGATCTG GATACCTTTA AGCTTTTAGA AGAAGAGGAT 
TTGGATGAAC TGAACATCCG AGATCCTGAG CACAGAGCTG TTCTCCTCAC AGCCGTGGAA 
CATCTACAGG AGTATGATAG TAACAGCGAC CAGTCAGGAT CTCAGGAGAA ACTTCTCATT 
GAAGGTCAAG GCCTAGCTGG ATGCTCTCCT CGGGATTCTG GCTGCTATGA AAGCAGTGAA 
AACCTGGAGA ATGGTAAAAC CCGAAGGACC TGTCTTCTGC CCTCAAAATC ATCAAGTGAG 
CATAGCTTTA AAGACTTCAG CAGACACCAG CTATCAAATT ATCCCACACT CCCACTGACC 
AAGTCAATAG AAACTCTACA GCAAGGAGAA AAAGAAAGCC GGTTGAGCTG TGCACATCGT 
GCTCTGAAGA GCTCTGTCAA GACCCCAGCT GTTATGGGTC TGAAGAAGAA CCGTAGAAGT 
TTACCAGTTG CTGTCTGTCG GAGCTATGAA ACTCTGGATG GCCCCCAGAG TGTGGATGTA 
TGGCCAAGAT CTCACTCCCT GGACGATCTC CAGGGAGATT CCGATACTAA TCTACAAGAT 
GGTAGCAAGA AAGATTATTT TGCTCGGGAT TCACTGGATA CAGCGAAAAA GGCAACTGGA 
TCTGCCCTGC CACCTCAGTC GTGTGGGGGC AGTTGTATGG TCGATAACTT GATGCCTAAG 
CAGGGTAAAG CAGCTGCTGG TTCTCAGAAG GGTAGAGGTA AGGAGTTGGA GTGCTCTGTA 
ATGGAGAGTG CAGTTGGGAA ACCTTTTCCA TTCCCCATGA AACACTGTGA AGCTCAGGCT 
GCCTTGGTGA CACATTCTGC AACAAGGACA CCTCTGGAAA TACAGAGTAA GGGCTTTCAT 
GACCTTGCAC GGGCTGACTA TGCTCCAGTC CTCAAGGGAG GTCTAGAAGC TGAGCAAAAA 
GGCACAAATG AGACAAGAAT GCAACCAAAA AATCCCTCTC AGCCGCCACC TGTTCCTGCC 
AAAAAATGCC GAGAACGCCT TACTAATGGA TTATATCATC CTCCCATGAC CCCCAGTGGG 
AACAACTCAA GTCAAGAAGC ACCGTGTTTG CCAGTAAAAA AGACCAGTGC ATCCAATCCC 
ACTGATTTTC ATGGAGTACC TGTCCATAGG ACATCTGAAC AGGAGCCCAG TACCCCTCCT 
AGCTCACTGC CACCCTGGCT GTTGGAGCAA CCAGAGACTA AAAGCGTTCA GCAGCACGTG 
ATAAAGCTAG GGCCTGCTTC AGCAAGGAAA GTCTCTTGTA GCAGGGGAAT GGATCTGGAA 
ATGGTAATAG AAAACAAGTT GCAGTCTGAA GGTATTGATC TTACTGAAGA ACCATACTCA 
GACAAGCATG GTCGCTGTGG CATTCCTGAG GCTTTGGTAC AGAGGTACTC CGAAGACTTA 
GACCAGCCTG AAAAGGATGT TGCTGCAAAC ATGGACCAAA TCCGGGTAAA GCAGCTGAGG 
AAGCAGCATC GCATGGCAAT TCCAAGTGGA GGGCTCACTG AAATTTGCCG AAAACCTGTA 
TCACCAGGAC TTATCACCTC CGTATCTGAC TGGCTGATTT CCATTGGCCT ACCCATGTAT 
TCCAGCCCAC TCACAGAAGC AGGGTTCAAC ACACTGAGCA AAGTGCCTTC TCTGTCACAA 
ACTTGCCTTC AGGAAGCTGG CATCACAGAG GAAAGGCACA TAAGCAAGCT CCTGGCAGCA 
GCAAGACTCT TCAAACCTCT CGATCCAGAC GCAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005137.1
CDS	131..4609
Translation	MCTNIVYEWLKTLQLPQYAESFVDNGYDDLEVCKQIGDPDLDAIGVAVPQHRRRIHEAVRRLREADERAAGLYFTLEPPPAPPAKGPRRPPGQDGAAPRHRPGRGGPVVYPRLKLKIMIRDKLVRDGINLSKPPYSNKDGSLGNIDDLAQQYADYYNTCFTDVCERMEELRKRRVSQDLDVEKPDASPTSLQLRSQIEESLGLSSTASTPDTERKLSIHKSSSEEGSVGKADWKKKNKFFWQNFRKNQKGLMRQTSKGEDVGYVASEITMSDEERIQLMMMVKEKMITIEEALARLKEYESQHRQSSTVDATDWPDGSYSTFDGSSNCNSREQSDDETEESVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGVEEQSLDNSAILDDRSALYSGVHKKQFYFDGSSEKQTEDDSDSLTTSPSSSSLDTWGASRKLVKTFSKTDSRGLIKPPKKLGTFFSYPEDEKTQKVCRSLTDGEMKKSLGSLSHGVSTESICFYDSNRHKHHLLVSLEEIQSVRKQIRLKKMDTNYSCSRAFLCQRPARKGASATTCDLQLLRHKVKGGGGCGFPNRRLRGRTSVSEFNITYVVERSLYSHLNLSQLVRPVTDRTLSKADKQDLRRCLLEEDEEAKRKWAATVDRCTKRVLLRIHQKSRTCSFGGFDLTNRSLHIGNNSDQMGKEGDFVYKEVIKSPSASRISLGKKVKSVKETMKKRMSKKYSSSLSEQESSPGVIPGSPQSPPPDTDSLDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRRKSRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVEHLQEYDSNSDQSGSQEKLLIEGQGLAGCSPRDSGCYESSENLENGKTRRTCLLPSKSSSEHSFKDFSRHQLSNYPTLPLTKSIETLQQGEKESRLSCAHRALKSSVKTPAVMGLKKNRRSLPVAVCRSYETLDGPQSVDVWPRSHSLDDLQGDSDTNLQDGSKKDYFARDSLDTAKKATGSALPPQSCGGSCMVDNLMPKQGKAAAGSQKGRGKELECSVMESAVGKPFPFPMKHCEAQAALVTHSATRTPLEIQSKGFHDLARADYAPVLKGGLEAEQKGTNETRMQPKNPSQPPPVPAKKCRERLTNGLYHPPMTPSGNNSSQEAPCLPVKKTSASNPTDFHGVPVHRTSEQEPSTPPSSLPPWLLEQPETKSVQQHVIKLGPASARKVSCSRGMDLEMVIENKLQSEGIDLTEEPYSDKHGRCGIPEALVQRYSEDLDQPEKDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGLITSVSDWLISIGLPMYSSPLTEAGFNTLSKVPSLSQTCLQEAGITEERHISKLLAAARLFKPLDPDAI

Target ORF information:

RefSeq Version	XM_025149369.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149369.1

ORF Insert Sequence:

ATGTGCACCA ACATAGTGTA CGAGTGGCTG AAAACCCTGC AGCTCCCCCA GTACGCCGAG 
TCCTTTGTCG ACAATGGCTA CGACGACCTG GAGGTCTGCA AGCAGATCGG CGACCCGGAC 
CTGGATGCCA TCGGCGTGGC CGTGCCCCAG CACCGCCGCC GCATCCACGA GGCCGTGCGG 
CGGCTGCGGG AGGCGGACGA GCGGGCGGCG GGGCTCTACT TCACCCTGGA GCCGCCGCCC 
GCGCCTCCCG CCAAGGGGCC CCGCCGCCCG CCGGGGCAGG ACGGGGCGGC CCCCCGGCAC 
CGGCCTGGCC GCGGGGGCCC GGTGGTCTAC CCCAGGCTGA AGCTGAAGAT CATGATCCGG 
GACAAGCTGG TCCGCGACGG CATCAACCTG AGCAAGCCCC CCTACTCCAA CAAGGATGGG 
TCTCTGGGAA ACATTGATGA CCTCGCACAG CAGTATGCAG ATTATTACAA CACCTGCTTC 
ACGGATGTGT GTGAGAGGAT GGAAGAGCTG CGCAAACGGA GGGTTTCCCA GGACCTTGAT 
GTGGAGAAAC CTGATGCCAG TCCCACATCT CTACAACTGC GATCCCAGAT TGAGGAGTCA 
CTTGGTCTCA GCAGCACCGC ATCAACACCT GACACTGAAA GAAAGCTCTC CATTCATAAA 
TCAAGTTCGG AAGAAGGCTC TGTAGGGAAA GCAGACTGGA AAAAGAAAAA TAAGTTTTTT 
TGGCAAAATT TCCGAAAGAA CCAGAAAGGA TTGATGAGGC AGACTTCAAA AGGAGAGGAT 
GTGGGGTATG TGGCCAGTGA GATTACGATG AGCGATGAGG AACGGATCCA GCTGATGATG 
ATGGTGAAAG AGAAGATGAT CACTATTGAG GAAGCACTTG CTAGGCTGAA GGAGTATGAG 
TCCCAGCACA GGCAATCAAG TACTGTAGAT GCTACAGACT GGCCTGATGG TTCTTACTCA 
ACATTTGATG GGTCTTCCAA TTGTAATTCA AGAGAACAAT CCGATGATGA AACAGAGGAG 
TCGGTGAAGT TTAAGAGATT GCACAAGCTG GTAAATTCTA CTCGCAGAGT CAGGAAGAAA 
CTCATAAGGG TGGAAGAAAT GAAAAAACCC AGTACAGAAG GCGTAGAGGA GCAGTCGCTT 
GACAACTCTG CTATACTGGA TGACAGATCG GCACTTTACT CTGGAGTTCA CAAGAAGCAA 
TTCTACTTTG ATGGTTCCTC CGAGAAGCAG ACAGAGGATG ACTCGGACTC ACTTACTACT 
TCTCCCTCAT CTAGTAGTCT TGATACATGG GGAGCTAGCC GGAAACTGGT GAAGACCTTC 
AGCAAAACTG ATAGCCGTGG CCTTATCAAG CCTCCCAAGA AACTTGGGAC ATTTTTTTCT 
TACCCAGAAG ATGAGAAGAC CCAGAAAGTT TGCCGCTCCT TGACAGATGG GGAGATGAAG 
AAGAGCCTCG GCTCACTGAG CCATGGGGTA AGTACAGAAA GCATTTGCTT TTATGACAGC 
AACAGGCACA AGCACCATCT TCTGGTGTCC CTGGAGGAGA TTCAGAGTGT AAGGAAGCAG 
ATCCGATTGA AGAAAATGGA TACTAACTAT TCCTGTTCCA GAGCTTTTCT TTGCCAGCGC 
CCTGCTAGGA AAGGAGCATC CGCCACAACT TGCGACCTAC AATTACTGCG GCACAAAGTG 
AAAGGGGGTG GAGGTTGTGG CTTCCCAAAC AGAAGGCTAC GAGGGCGCAC TTCTGTCAGC 
GAGTTCAATA TTACCTATGT GGTGGAGAGG AGCCTGTACA GTCACCTCAA CCTCTCACAG 
CTGGTGCGTC CCGTTACTGA CAGGACCCTG AGCAAAGCTG ACAAGCAGGA CCTGAGGAGA 
TGCCTGCTAG AGGAGGATGA GGAGGCCAAG AGGAAGTGGG CTGCCACAGT GGATCGTTGT 
ACTAAAAGGG TTCTCTTGAG AATCCACCAA AAGTCTAGAA CCTGCAGCTT TGGAGGATTT 
GACTTGACAA ATCGTTCCCT TCATATTGGA AACAATTCTG ACCAAATGGG CAAAGAAGGT 
GACTTTGTTT ATAAAGAAGT AATCAAATCA CCCTCTGCCT CCCGTATATC TCTGGGCAAA 
AAGGTGAAAT CTGTGAAAGA AACCATGAAG AAAAGGATGT CTAAAAAATA CAGCAGCTCT 
CTGTCTGAGC AGGAGTCGAG CCCTGGAGTC ATACCGGGTT CTCCGCAGTC GCCGCCGCCA 
GACACTGACT CTCTGGACAA GCCCAAGCTG AAAGCTGGTG GCTCAGTTGA GAGCCTGAGG 
AGTTCCTTAA GTGGTCAGAG CTCAATGAGT GGTCAGACAG TGAGTACAAC AGATTCCTCA 
ACCAGCAACA GGGAGAGCGT GAAATCAGAA GATGGGGATG ATGAAGAACC TCCCTACAGA 
GGACCCTTCT GTGGAAGAGC CAGGGTTCAC ACTGATTTTA CTCCAAGTCC TTATGATACA 
GACTCTCTGA AGCTCAAGAA AGGTGATATC ATTGACATCA TCAGCAAACC CCCCATGGGC 
ACTTGGATGG GCCTATTGAA CAACAAAGTT GGCACTTTCA AATTTATCTA TGTGGATGTG 
TTAAATGAAG AGGAAGAGAA GCCGAAGCGA CCCACCAGGA GACGCCGAAA AAGCAGACCA 
CCCCAACCCA AGTCAGTTGA GGATCTCTTG GATCGCATCA ACTTAAAGGA GCACATGCCC 
ACTTTTCTGT TCAATGGTTA TGAAGATCTG GATACCTTTA AGCTTTTAGA AGAAGAGGAT 
TTGGATGAAC TGAACATCCG AGATCCTGAG CACAGAGCTG TTCTCCTCAC AGCCGTGGAA 
CATCTACAGG AGTATGATAG TAACAGCGAC CAGTCAGGAT CTCAGGAGAA ACTTCTCATT 
GAAGGTCAAG GCCTAGCTGG ATGCTCTCCT CGGGATTCTG GCTGCTATGA AAGCAGTGAA 
AACCTGGAGA ATGGTAAAAC CCGAAGGACC TGTCTTCTGC CCTCAAAATC ATCAAGTGAG 
CATAGCTTTA AAGACTTCAG CAGACACCAG CTATCAAATT ATCCCACACT CCCACTGACC 
AAGTCAATAG AAACTCTACA GCAAGGAGAA AAAGAAAGCC GGTTGAGCTG TGCACATCGT 
GCTCTGAAGA GCTCTGTCAA GACCCCAGCT GTTATGGGTC TGAAGAAGAA CCGTAGAAGT 
TTACCAGTTG CTGTCTGTCG GAGCTATGAA ACTCTGGATG GCCCCCAGAG TGTGGATGTA 
TGGCCAAGAT CTCACTCCCT GGACGATCTC CAGGGAGATT CCGATACTAA TCTACAAGAT 
GGTAGCAAGA AAGATTATTT TGCTCGGGAT TCACTGGATA CAGCGAAAAA GGCAACTGGA 
TCTGCCCTGC CACCTCAGTC GTGTGGGGGC AGTTGTATGG TCGATAACTT GATGCCTAAG 
CAGGGTAAAG CAGCTGCTGG TTCTCAGAAG GGTAGAGGTA AGGAGTTGGA GTGCTCTGTA 
ATGGAGAGTG CAGTTGGGAA ACCTTTTCCA TTCCCCATGA AACACTGTGA AGCTCAGGCT 
GCCTTGGTGA CACATTCTGC AACAAGGACA CCTCTGGAAA TACAGAGTAA GGGCTTTCAT 
GACCTTGCAC GGGCTGACTA TGCTCCAGTC CTCAAGGGAG GTCTAGAAGC TGAGCAAAAA 
GGCACAAATG AGACAAGAAT GCAACCAAAA AATCCCTCTC AGCCGCCACC TGTTCCTGCC 
AAAAAATGCC GAGAACGCCT TACTAATGGA TTATATCATC CTCCCATGAC CCCCAGTGGG 
AACAACTCAA GTCAAGAAGC ACCGTGTTTG CCAGTAAAAA AGACCAGTGC ATCCAATCCC 
ACTGATTTTC ATGGAGTACC TGTCCATAGG ACATCTGAAC AGGAGCCCAG TACCCCTCCT 
AGCTCACTGC CACCCTGGCT GTTGGAGCAA CCAGAGACTA AAAGCGTTCA GCAGCACGTG 
ATAAAGCTAG GGCCTGCTTC AGCAAGGAAA GTCTCTTGTA GCAGGGGAAT GGATCTGGAA 
ATGGTAATAG AAAACAAGTT GCAGTCTGAA GGTATTGATC TTACTGAAGA ACCATACTCA 
GACAAGCATG GTCGCTGTGG CATTCCTGAG GCTTTGGTAC AGAGGTACTC CGAAGACTTA 
GACCAGCCTG AAAAGGATGT TGCTGCAAAC ATGGACCAAA TCCGGGTAAA GCAGCTGAGG 
AAGCAGCATC GCATGGCAAT TCCAAGTGGA GGGCTCACTG AAATTTGCCG AAAACCTGTA 
TCACCAGGAC TTATCACCTC CGTATCTGAC TGGCTGATTT CCATTGGCCT ACCCATGTAT 
TCCAGCCCAC TCACAGAAGC AGGGTTCAAC ACACTGAGCA AAGTGCCTTC TCTGTCACAA 
ACTTGCCTTC AGGAAGCTGG CATCACAGAG GAAAGGCACA TAAGCAAGCT CCTGGCAGCA 
GCAAGACTCT TCAAACCTCT CGATCCAGAC GCAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH1 ( XM_025149370.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005138.1 Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa149132
Clone ID Related Accession (Same CDS sequence)	XM_025149370.1
Accession Version	XM_025149370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	SAM and SH3 domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACCA ACATAGTGTA CGAGTGGCTG AAAACCCTGC AGCTCCCCCA GTACGCCGAG 
TCCTTTGTCG ACAATGGCTA CGACGACCTG GAGGTCTGCA AGCAGATCGG CGACCCGGAC 
CTGGATGCCA TCGGCGTGGC CGTGCCCCAG CACCGCCGCC GCATCCACGA GGCCGTGCGG 
CGGCTGCGGG AGGCGGACGA GCGGGCGGCG GGGCTCTACT TCACCCTGGA GCCGCCGCCC 
GCGCCTCCCG CCAAGGGGCC CCGCCGCCCG CCGGGGCAGG ACGGGGCGGC CCCCCGGCAC 
CGGCCTGGCC GCGGGGGCCC GGTGGTCTAC CCCAGGCTGA AGCTGAAGAT CATGATCCGG 
GACAAGCTGG TCCGCGACGG CATCAACCTG AGCAAGCCCC CCTACTCCAA CAAGGATGGG 
TCTCTGGGAA ACATTGATGA CCTCGCACAG CAGTATGCAG ATTATTACAA CACCTGCTTC 
ACGGATGTGT GTGAGAGGAT GGAAGAGCTG CGCAAACGGA GGGTTTCCCA GGACCTTGAT 
GTGGAGAAAC CTGATGCCAG TCCCACATCT CTACAACTGC GATCCCAGAT TGAGGAGTCA 
CTTGGTCTCA GCAGCACCGC ATCAACACCT GACACTGAAA GAAAGCTCTC CATTCATAAA 
TCAAGTTCGG AAGAAGGCTC TGTAGACTGG AAAAAGAAAA ATAAGTTTTT TTGGCAAAAT 
TTCCGAAAGA ACCAGAAAGG ATTGATGAGG CAGACTTCAA AAGGAGAGGA TGTGGGGTAT 
GTGGCCAGTG AGATTACGAT GAGCGATGAG GAACGGATCC AGCTGATGAT GATGGTGAAA 
GAGAAGATGA TCACTATTGA GGAAGCACTT GCTAGGCTGA AGGAGTATGA GTCCCAGCAC 
AGGCAATCAA GTACTGTAGA TGCTACAGAC TGGCCTGATG GTTCTTACTC AACATTTGAT 
GGGTCTTCCA ATTGTAATTC AAGAGAACAA TCCGATGATG AAACAGAGGA GTCGGTGAAG 
TTTAAGAGAT TGCACAAGCT GGTAAATTCT ACTCGCAGAG TCAGGAAGAA ACTCATAAGG 
GTGGAAGAAA TGAAAAAACC CAGTACAGAA GGCGTAGAGG AGCAGTCGCT TGACAACTCT 
GCTATACTGG ATGACAGATC GGCACTTTAC TCTGGAGTTC ACAAGAAGCA ATTCTACTTT 
GATGGTTCCT CCGAGAAGCA GACAGAGGAT GACTCGGACT CACTTACTAC TTCTCCCTCA 
TCTAGTAGTC TTGATACATG GGGAGCTAGC CGGAAACTGG TGAAGACCTT CAGCAAAACT 
GATAGCCGTG GCCTTATCAA GCCTCCCAAG AAACTTGGGA CATTTTTTTC TTACCCAGAA 
GATGAGAAGA CCCAGAAAGT TTGCCGCTCC TTGACAGATG GGGAGATGAA GAAGAGCCTC 
GGCTCACTGA GCCATGGGGT AAGTACAGAA AGCATTTGCT TTTATGACAG CAACAGGCAC 
AAGCACCATC TTCTGGTGTC CCTGGAGGAG ATTCAGAGTG TAAGGAAGCA GATCCGATTG 
AAGAAAATGG ATACTAACTA TTCCTGTTCC AGAGCTTTTC TTTGCCAGCG CCCTGCTAGG 
AAAGGAGCAT CCGCCACAAC TTGCGACCTA CAATTACTGC GGCACAAAGT GAAAGGGGGT 
GGAGGTTGTG GCTTCCCAAA CAGAAGGCTA CGAGGGCGCA CTTCTGTCAG CGAGTTCAAT 
ATTACCTATG TGGTGGAGAG GAGCCTGTAC AGTCACCTCA ACCTCTCACA GCTGGTGCGT 
CCCGTTACTG ACAGGACCCT GAGCAAAGCT GACAAGCAGG ACCTGAGGAG ATGCCTGCTA 
GAGGAGGATG AGGAGGCCAA GAGGAAGTGG GCTGCCACAG TGGATCGTTG TACTAAAAGG 
GTTCTCTTGA GAATCCACCA AAAGTCTAGA ACCTGCAGCT TTGGAGGATT TGACTTGACA 
AATCGTTCCC TTCATATTGG AAACAATTCT GACCAAATGG GCAAAGAAGG TGACTTTGTT 
TATAAAGAAG TAATCAAATC ACCCTCTGCC TCCCGTATAT CTCTGGGCAA AAAGGTGAAA 
TCTGTGAAAG AAACCATGAA GAAAAGGATG TCTAAAAAAT ACAGCAGCTC TCTGTCTGAG 
CAGGAGTCGA GCCCTGGAGT CATACCGGGT TCTCCGCAGT CGCCGCCGCC AGACACTGAC 
TCTCTGGACA AGCCCAAGCT GAAAGCTGGT GGCTCAGTTG AGAGCCTGAG GAGTTCCTTA 
AGTGGTCAGA GCTCAATGAG TGGTCAGACA GTGAGTACAA CAGATTCCTC AACCAGCAAC 
AGGGAGAGCG TGAAATCAGA AGATGGGGAT GATGAAGAAC CTCCCTACAG AGGACCCTTC 
TGTGGAAGAG CCAGGGTTCA CACTGATTTT ACTCCAAGTC CTTATGATAC AGACTCTCTG 
AAGCTCAAGA AAGGTGATAT CATTGACATC ATCAGCAAAC CCCCCATGGG CACTTGGATG 
GGCCTATTGA ACAACAAAGT TGGCACTTTC AAATTTATCT ATGTGGATGT GTTAAATGAA 
GAGGAAGAGA AGCCGAAGCG ACCCACCAGG AGACGCCGAA AAAGCAGACC ACCCCAACCC 
AAGTCAGTTG AGGATCTCTT GGATCGCATC AACTTAAAGG AGCACATGCC CACTTTTCTG 
TTCAATGGTT ATGAAGATCT GGATACCTTT AAGCTTTTAG AAGAAGAGGA TTTGGATGAA 
CTGAACATCC GAGATCCTGA GCACAGAGCT GTTCTCCTCA CAGCCGTGGA ACATCTACAG 
GAGTATGATA GTAACAGCGA CCAGTCAGGA TCTCAGGAGA AACTTCTCAT TGAAGGTCAA 
GGCCTAGCTG GATGCTCTCC TCGGGATTCT GGCTGCTATG AAAGCAGTGA AAACCTGGAG 
AATGGTAAAA CCCGAAGGAC CTGTCTTCTG CCCTCAAAAT CATCAAGTGA GCATAGCTTT 
AAAGACTTCA GCAGACACCA GCTATCAAAT TATCCCACAC TCCCACTGAC CAAGTCAATA 
GAAACTCTAC AGCAAGGAGA AAAAGAAAGC CGGTTGAGCT GTGCACATCG TGCTCTGAAG 
AGCTCTGTCA AGACCCCAGC TGTTATGGGT CTGAAGAAGA ACCGTAGAAG TTTACCAGTT 
GCTGTCTGTC GGAGCTATGA AACTCTGGAT GGCCCCCAGA GTGTGGATGT ATGGCCAAGA 
TCTCACTCCC TGGACGATCT CCAGGGAGAT TCCGATACTA ATCTACAAGA TGGTAGCAAG 
AAAGATTATT TTGCTCGGGA TTCACTGGAT ACAGCGAAAA AGGCAACTGG ATCTGCCCTG 
CCACCTCAGT CGTGTGGGGG CAGTTGTATG GTCGATAACT TGATGCCTAA GCAGGGTAAA 
GCAGCTGCTG GTTCTCAGAA GGGTAGAGGT AAGGAGTTGG AGTGCTCTGT AATGGAGAGT 
GCAGTTGGGA AACCTTTTCC ATTCCCCATG AAACACTGTG AAGCTCAGGC TGCCTTGGTG 
ACACATTCTG CAACAAGGAC ACCTCTGGAA ATACAGAGTA AGGGCTTTCA TGACCTTGCA 
CGGGCTGACT ATGCTCCAGT CCTCAAGGGA GGTCTAGAAG CTGAGCAAAA AGGCACAAAT 
GAGACAAGAA TGCAACCAAA AAATCCCTCT CAGCCGCCAC CTGTTCCTGC CAAAAAATGC 
CGAGAACGCC TTACTAATGG ATTATATCAT CCTCCCATGA CCCCCAGTGG GAACAACTCA 
AGTCAAGAAG CACCGTGTTT GCCAGTAAAA AAGACCAGTG CATCCAATCC CACTGATTTT 
CATGGAGTAC CTGTCCATAG GACATCTGAA CAGGAGCCCA GTACCCCTCC TAGCTCACTG 
CCACCCTGGC TGTTGGAGCA ACCAGAGACT AAAAGCGTTC AGCAGCACGT GATAAAGCTA 
GGGCCTGCTT CAGCAAGGAA AGTCTCTTGT AGCAGGGGAA TGGATCTGGA AATGGTAATA 
GAAAACAAGT TGCAGTCTGA AGGTATTGAT CTTACTGAAG AACCATACTC AGACAAGCAT 
GGTCGCTGTG GCATTCCTGA GGCTTTGGTA CAGAGGTACT CCGAAGACTT AGACCAGCCT 
GAAAAGGATG TTGCTGCAAA CATGGACCAA ATCCGGGTAA AGCAGCTGAG GAAGCAGCAT 
CGCATGGCAA TTCCAAGTGG AGGGCTCACT GAAATTTGCC GAAAACCTGT ATCACCAGGA 
CTTATCACCT CCGTATCTGA CTGGCTGATT TCCATTGGCC TACCCATGTA TTCCAGCCCA 
CTCACAGAAG CAGGGTTCAA CACACTGAGC AAAGTGCCTT CTCTGTCACA AACTTGCCTT 
CAGGAAGCTG GCATCACAGA GGAAAGGCAC ATAAGCAAGC TCCTGGCAGC AGCAAGACTC 
TTCAAACCTC TCGATCCAGA CGCAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005138.1
CDS	131..4600
Translation	MCTNIVYEWLKTLQLPQYAESFVDNGYDDLEVCKQIGDPDLDAIGVAVPQHRRRIHEAVRRLREADERAAGLYFTLEPPPAPPAKGPRRPPGQDGAAPRHRPGRGGPVVYPRLKLKIMIRDKLVRDGINLSKPPYSNKDGSLGNIDDLAQQYADYYNTCFTDVCERMEELRKRRVSQDLDVEKPDASPTSLQLRSQIEESLGLSSTASTPDTERKLSIHKSSSEEGSVDWKKKNKFFWQNFRKNQKGLMRQTSKGEDVGYVASEITMSDEERIQLMMMVKEKMITIEEALARLKEYESQHRQSSTVDATDWPDGSYSTFDGSSNCNSREQSDDETEESVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGVEEQSLDNSAILDDRSALYSGVHKKQFYFDGSSEKQTEDDSDSLTTSPSSSSLDTWGASRKLVKTFSKTDSRGLIKPPKKLGTFFSYPEDEKTQKVCRSLTDGEMKKSLGSLSHGVSTESICFYDSNRHKHHLLVSLEEIQSVRKQIRLKKMDTNYSCSRAFLCQRPARKGASATTCDLQLLRHKVKGGGGCGFPNRRLRGRTSVSEFNITYVVERSLYSHLNLSQLVRPVTDRTLSKADKQDLRRCLLEEDEEAKRKWAATVDRCTKRVLLRIHQKSRTCSFGGFDLTNRSLHIGNNSDQMGKEGDFVYKEVIKSPSASRISLGKKVKSVKETMKKRMSKKYSSSLSEQESSPGVIPGSPQSPPPDTDSLDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRRKSRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVEHLQEYDSNSDQSGSQEKLLIEGQGLAGCSPRDSGCYESSENLENGKTRRTCLLPSKSSSEHSFKDFSRHQLSNYPTLPLTKSIETLQQGEKESRLSCAHRALKSSVKTPAVMGLKKNRRSLPVAVCRSYETLDGPQSVDVWPRSHSLDDLQGDSDTNLQDGSKKDYFARDSLDTAKKATGSALPPQSCGGSCMVDNLMPKQGKAAAGSQKGRGKELECSVMESAVGKPFPFPMKHCEAQAALVTHSATRTPLEIQSKGFHDLARADYAPVLKGGLEAEQKGTNETRMQPKNPSQPPPVPAKKCRERLTNGLYHPPMTPSGNNSSQEAPCLPVKKTSASNPTDFHGVPVHRTSEQEPSTPPSSLPPWLLEQPETKSVQQHVIKLGPASARKVSCSRGMDLEMVIENKLQSEGIDLTEEPYSDKHGRCGIPEALVQRYSEDLDQPEKDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGLITSVSDWLISIGLPMYSSPLTEAGFNTLSKVPSLSQTCLQEAGITEERHISKLLAAARLFKPLDPDAI

Target ORF information:

RefSeq Version	XM_025149370.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149370.1

ORF Insert Sequence:

ATGTGCACCA ACATAGTGTA CGAGTGGCTG AAAACCCTGC AGCTCCCCCA GTACGCCGAG 
TCCTTTGTCG ACAATGGCTA CGACGACCTG GAGGTCTGCA AGCAGATCGG CGACCCGGAC 
CTGGATGCCA TCGGCGTGGC CGTGCCCCAG CACCGCCGCC GCATCCACGA GGCCGTGCGG 
CGGCTGCGGG AGGCGGACGA GCGGGCGGCG GGGCTCTACT TCACCCTGGA GCCGCCGCCC 
GCGCCTCCCG CCAAGGGGCC CCGCCGCCCG CCGGGGCAGG ACGGGGCGGC CCCCCGGCAC 
CGGCCTGGCC GCGGGGGCCC GGTGGTCTAC CCCAGGCTGA AGCTGAAGAT CATGATCCGG 
GACAAGCTGG TCCGCGACGG CATCAACCTG AGCAAGCCCC CCTACTCCAA CAAGGATGGG 
TCTCTGGGAA ACATTGATGA CCTCGCACAG CAGTATGCAG ATTATTACAA CACCTGCTTC 
ACGGATGTGT GTGAGAGGAT GGAAGAGCTG CGCAAACGGA GGGTTTCCCA GGACCTTGAT 
GTGGAGAAAC CTGATGCCAG TCCCACATCT CTACAACTGC GATCCCAGAT TGAGGAGTCA 
CTTGGTCTCA GCAGCACCGC ATCAACACCT GACACTGAAA GAAAGCTCTC CATTCATAAA 
TCAAGTTCGG AAGAAGGCTC TGTAGACTGG AAAAAGAAAA ATAAGTTTTT TTGGCAAAAT 
TTCCGAAAGA ACCAGAAAGG ATTGATGAGG CAGACTTCAA AAGGAGAGGA TGTGGGGTAT 
GTGGCCAGTG AGATTACGAT GAGCGATGAG GAACGGATCC AGCTGATGAT GATGGTGAAA 
GAGAAGATGA TCACTATTGA GGAAGCACTT GCTAGGCTGA AGGAGTATGA GTCCCAGCAC 
AGGCAATCAA GTACTGTAGA TGCTACAGAC TGGCCTGATG GTTCTTACTC AACATTTGAT 
GGGTCTTCCA ATTGTAATTC AAGAGAACAA TCCGATGATG AAACAGAGGA GTCGGTGAAG 
TTTAAGAGAT TGCACAAGCT GGTAAATTCT ACTCGCAGAG TCAGGAAGAA ACTCATAAGG 
GTGGAAGAAA TGAAAAAACC CAGTACAGAA GGCGTAGAGG AGCAGTCGCT TGACAACTCT 
GCTATACTGG ATGACAGATC GGCACTTTAC TCTGGAGTTC ACAAGAAGCA ATTCTACTTT 
GATGGTTCCT CCGAGAAGCA GACAGAGGAT GACTCGGACT CACTTACTAC TTCTCCCTCA 
TCTAGTAGTC TTGATACATG GGGAGCTAGC CGGAAACTGG TGAAGACCTT CAGCAAAACT 
GATAGCCGTG GCCTTATCAA GCCTCCCAAG AAACTTGGGA CATTTTTTTC TTACCCAGAA 
GATGAGAAGA CCCAGAAAGT TTGCCGCTCC TTGACAGATG GGGAGATGAA GAAGAGCCTC 
GGCTCACTGA GCCATGGGGT AAGTACAGAA AGCATTTGCT TTTATGACAG CAACAGGCAC 
AAGCACCATC TTCTGGTGTC CCTGGAGGAG ATTCAGAGTG TAAGGAAGCA GATCCGATTG 
AAGAAAATGG ATACTAACTA TTCCTGTTCC AGAGCTTTTC TTTGCCAGCG CCCTGCTAGG 
AAAGGAGCAT CCGCCACAAC TTGCGACCTA CAATTACTGC GGCACAAAGT GAAAGGGGGT 
GGAGGTTGTG GCTTCCCAAA CAGAAGGCTA CGAGGGCGCA CTTCTGTCAG CGAGTTCAAT 
ATTACCTATG TGGTGGAGAG GAGCCTGTAC AGTCACCTCA ACCTCTCACA GCTGGTGCGT 
CCCGTTACTG ACAGGACCCT GAGCAAAGCT GACAAGCAGG ACCTGAGGAG ATGCCTGCTA 
GAGGAGGATG AGGAGGCCAA GAGGAAGTGG GCTGCCACAG TGGATCGTTG TACTAAAAGG 
GTTCTCTTGA GAATCCACCA AAAGTCTAGA ACCTGCAGCT TTGGAGGATT TGACTTGACA 
AATCGTTCCC TTCATATTGG AAACAATTCT GACCAAATGG GCAAAGAAGG TGACTTTGTT 
TATAAAGAAG TAATCAAATC ACCCTCTGCC TCCCGTATAT CTCTGGGCAA AAAGGTGAAA 
TCTGTGAAAG AAACCATGAA GAAAAGGATG TCTAAAAAAT ACAGCAGCTC TCTGTCTGAG 
CAGGAGTCGA GCCCTGGAGT CATACCGGGT TCTCCGCAGT CGCCGCCGCC AGACACTGAC 
TCTCTGGACA AGCCCAAGCT GAAAGCTGGT GGCTCAGTTG AGAGCCTGAG GAGTTCCTTA 
AGTGGTCAGA GCTCAATGAG TGGTCAGACA GTGAGTACAA CAGATTCCTC AACCAGCAAC 
AGGGAGAGCG TGAAATCAGA AGATGGGGAT GATGAAGAAC CTCCCTACAG AGGACCCTTC 
TGTGGAAGAG CCAGGGTTCA CACTGATTTT ACTCCAAGTC CTTATGATAC AGACTCTCTG 
AAGCTCAAGA AAGGTGATAT CATTGACATC ATCAGCAAAC CCCCCATGGG CACTTGGATG 
GGCCTATTGA ACAACAAAGT TGGCACTTTC AAATTTATCT ATGTGGATGT GTTAAATGAA 
GAGGAAGAGA AGCCGAAGCG ACCCACCAGG AGACGCCGAA AAAGCAGACC ACCCCAACCC 
AAGTCAGTTG AGGATCTCTT GGATCGCATC AACTTAAAGG AGCACATGCC CACTTTTCTG 
TTCAATGGTT ATGAAGATCT GGATACCTTT AAGCTTTTAG AAGAAGAGGA TTTGGATGAA 
CTGAACATCC GAGATCCTGA GCACAGAGCT GTTCTCCTCA CAGCCGTGGA ACATCTACAG 
GAGTATGATA GTAACAGCGA CCAGTCAGGA TCTCAGGAGA AACTTCTCAT TGAAGGTCAA 
GGCCTAGCTG GATGCTCTCC TCGGGATTCT GGCTGCTATG AAAGCAGTGA AAACCTGGAG 
AATGGTAAAA CCCGAAGGAC CTGTCTTCTG CCCTCAAAAT CATCAAGTGA GCATAGCTTT 
AAAGACTTCA GCAGACACCA GCTATCAAAT TATCCCACAC TCCCACTGAC CAAGTCAATA 
GAAACTCTAC AGCAAGGAGA AAAAGAAAGC CGGTTGAGCT GTGCACATCG TGCTCTGAAG 
AGCTCTGTCA AGACCCCAGC TGTTATGGGT CTGAAGAAGA ACCGTAGAAG TTTACCAGTT 
GCTGTCTGTC GGAGCTATGA AACTCTGGAT GGCCCCCAGA GTGTGGATGT ATGGCCAAGA 
TCTCACTCCC TGGACGATCT CCAGGGAGAT TCCGATACTA ATCTACAAGA TGGTAGCAAG 
AAAGATTATT TTGCTCGGGA TTCACTGGAT ACAGCGAAAA AGGCAACTGG ATCTGCCCTG 
CCACCTCAGT CGTGTGGGGG CAGTTGTATG GTCGATAACT TGATGCCTAA GCAGGGTAAA 
GCAGCTGCTG GTTCTCAGAA GGGTAGAGGT AAGGAGTTGG AGTGCTCTGT AATGGAGAGT 
GCAGTTGGGA AACCTTTTCC ATTCCCCATG AAACACTGTG AAGCTCAGGC TGCCTTGGTG 
ACACATTCTG CAACAAGGAC ACCTCTGGAA ATACAGAGTA AGGGCTTTCA TGACCTTGCA 
CGGGCTGACT ATGCTCCAGT CCTCAAGGGA GGTCTAGAAG CTGAGCAAAA AGGCACAAAT 
GAGACAAGAA TGCAACCAAA AAATCCCTCT CAGCCGCCAC CTGTTCCTGC CAAAAAATGC 
CGAGAACGCC TTACTAATGG ATTATATCAT CCTCCCATGA CCCCCAGTGG GAACAACTCA 
AGTCAAGAAG CACCGTGTTT GCCAGTAAAA AAGACCAGTG CATCCAATCC CACTGATTTT 
CATGGAGTAC CTGTCCATAG GACATCTGAA CAGGAGCCCA GTACCCCTCC TAGCTCACTG 
CCACCCTGGC TGTTGGAGCA ACCAGAGACT AAAAGCGTTC AGCAGCACGT GATAAAGCTA 
GGGCCTGCTT CAGCAAGGAA AGTCTCTTGT AGCAGGGGAA TGGATCTGGA AATGGTAATA 
GAAAACAAGT TGCAGTCTGA AGGTATTGAT CTTACTGAAG AACCATACTC AGACAAGCAT 
GGTCGCTGTG GCATTCCTGA GGCTTTGGTA CAGAGGTACT CCGAAGACTT AGACCAGCCT 
GAAAAGGATG TTGCTGCAAA CATGGACCAA ATCCGGGTAA AGCAGCTGAG GAAGCAGCAT 
CGCATGGCAA TTCCAAGTGG AGGGCTCACT GAAATTTGCC GAAAACCTGT ATCACCAGGA 
CTTATCACCT CCGTATCTGA CTGGCTGATT TCCATTGGCC TACCCATGTA TTCCAGCCCA 
CTCACAGAAG CAGGGTTCAA CACACTGAGC AAAGTGCCTT CTCTGTCACA AACTTGCCTT 
CAGGAAGCTG GCATCACAGA GGAAAGGCAC ATAAGCAAGC TCCTGGCAGC AGCAAGACTC 
TTCAAACCTC TCGATCCAGA CGCAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH1 ( XM_025149371.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005139.1 Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X3, mRNA.

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CloneID	OGa149133
Clone ID Related Accession (Same CDS sequence)	XM_025149371.1
Accession Version	XM_025149371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	SAM and SH3 domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACCA ACATAGTGTA CGAGTGGCTG AAAACCCTGC AGCTCCCCCA GTACGCCGAG 
TCCTTTGTCG ACAATGGCTA CGACGACCTG GAGGTCTGCA AGCAGATCGG CGACCCGGAC 
CTGGATGCCA TCGGCGTGGC CGTGCCCCAG CACCGCCGCC GCATCCACGA GGCCGTGCGG 
CGGCTGCGGG AGGCGGACGA GCGGGCGGCG GGGCTCTACT TCACCCTGGA GCCGCCGCCC 
GCGCCTCCCG CCAAGGGGCC CCGCCGCCCG CCGGGGCAGG ACGGGGCGGC CCCCCGGCAC 
CGGCCTGGCC GCGGGGGCCC GGTGGTCTAC CCCAGGCTGA AGCTGAAGAT CATGATCCGG 
GACAAGCTGG TCCGCGACGG CATCAACCTG AGCAAGCCCC CCTACTCCAA CAAGGATGGG 
TCTCTGGGAA ACATTGATGA CCTCGCACAG CAGTATGCAG ATTATTACAA CACCTGCTTC 
ACGGATGTGT GTGAGAGGAT GGAAGAGCTG CGCAAACGGA GGGTTTCCCA GGACCTTGAT 
GTGGAGAAAC CTGATGCCAG TCCCACATCT CTACAACTGC GATCCCAGAT TGAGGAGTCA 
CTTGGTCTCA GCAGCACCGC ATCAACACCT GACACTGAAA GAAAGCTCTC CATTCATAAA 
TCAAGTTCGG AAGAAGGCTC TGTAGGGAAA GCAGACTGGA AAAAGAAAAA TAAGTTTTTT 
TGGCAAAATT TCCGAAAGAA CCAGAAAGGA TTGATGAGGC AGACTTCAAA AGGAGAGGAT 
GTGGGGTATG TGGCCAGTGA GATTACGATG AGCGATGAGG AACGGATCCA GCTGATGATG 
ATGGTGAAAG AGAAGATGAT CACTATTGAG GAAGCACTTG CTAGGTCAAG AGAACAATCC 
GATGATGAAA CAGAGGAGTC GGTGAAGTTT AAGAGATTGC ACAAGCTGGT AAATTCTACT 
CGCAGAGTCA GGAAGAAACT CATAAGGGTG GAAGAAATGA AAAAACCCAG TACAGAAGGC 
GTAGAGGAGC AGTCGCTTGA CAACTCTGCT ATACTGGATG ACAGATCGGC ACTTTACTCT 
GGAGTTCACA AGAAGCAATT CTACTTTGAT GGTTCCTCCG AGAAGCAGAC AGAGGATGAC 
TCGGACTCAC TTACTACTTC TCCCTCATCT AGTAGTCTTG ATACATGGGG AGCTAGCCGG 
AAACTGGTGA AGACCTTCAG CAAAACTGAT AGCCGTGGCC TTATCAAGCC TCCCAAGAAA 
CTTGGGACAT TTTTTTCTTA CCCAGAAGAT GAGAAGACCC AGAAAGTTTG CCGCTCCTTG 
ACAGATGGGG AGATGAAGAA GAGCCTCGGC TCACTGAGCC ATGGGGTAAG TACAGAAAGC 
ATTTGCTTTT ATGACAGCAA CAGGCACAAG CACCATCTTC TGGTGTCCCT GGAGGAGATT 
CAGAGTGTAA GGAAGCAGAT CCGATTGAAG AAAATGGATA CTAACTATTC CTGTTCCAGA 
GCTTTTCTTT GCCAGCGCCC TGCTAGGAAA GGAGCATCCG CCACAACTTG CGACCTACAA 
TTACTGCGGC ACAAAGTGAA AGGGGGTGGA GGTTGTGGCT TCCCAAACAG AAGGCTACGA 
GGGCGCACTT CTGTCAGCGA GTTCAATATT ACCTATGTGG TGGAGAGGAG CCTGTACAGT 
CACCTCAACC TCTCACAGCT GGTGCGTCCC GTTACTGACA GGACCCTGAG CAAAGCTGAC 
AAGCAGGACC TGAGGAGATG CCTGCTAGAG GAGGATGAGG AGGCCAAGAG GAAGTGGGCT 
GCCACAGTGG ATCGTTGTAC TAAAAGGGTT CTCTTGAGAA TCCACCAAAA GTCTAGAACC 
TGCAGCTTTG GAGGATTTGA CTTGACAAAT CGTTCCCTTC ATATTGGAAA CAATTCTGAC 
CAAATGGGCA AAGAAGGTGA CTTTGTTTAT AAAGAAGTAA TCAAATCACC CTCTGCCTCC 
CGTATATCTC TGGGCAAAAA GGTGAAATCT GTGAAAGAAA CCATGAAGAA AAGGATGTCT 
AAAAAATACA GCAGCTCTCT GTCTGAGCAG GAGTCGAGCC CTGGAGTCAT ACCGGGTTCT 
CCGCAGTCGC CGCCGCCAGA CACTGACTCT CTGGACAAGC CCAAGCTGAA AGCTGGTGGC 
TCAGTTGAGA GCCTGAGGAG TTCCTTAAGT GGTCAGAGCT CAATGAGTGG TCAGACAGTG 
AGTACAACAG ATTCCTCAAC CAGCAACAGG GAGAGCGTGA AATCAGAAGA TGGGGATGAT 
GAAGAACCTC CCTACAGAGG ACCCTTCTGT GGAAGAGCCA GGGTTCACAC TGATTTTACT 
CCAAGTCCTT ATGATACAGA CTCTCTGAAG CTCAAGAAAG GTGATATCAT TGACATCATC 
AGCAAACCCC CCATGGGCAC TTGGATGGGC CTATTGAACA ACAAAGTTGG CACTTTCAAA 
TTTATCTATG TGGATGTGTT AAATGAAGAG GAAGAGAAGC CGAAGCGACC CACCAGGAGA 
CGCCGAAAAA GCAGACCACC CCAACCCAAG TCAGTTGAGG ATCTCTTGGA TCGCATCAAC 
TTAAAGGAGC ACATGCCCAC TTTTCTGTTC AATGGTTATG AAGATCTGGA TACCTTTAAG 
CTTTTAGAAG AAGAGGATTT GGATGAACTG AACATCCGAG ATCCTGAGCA CAGAGCTGTT 
CTCCTCACAG CCGTGGAACA TCTACAGGAG TATGATAGTA ACAGCGACCA GTCAGGATCT 
CAGGAGAAAC TTCTCATTGA AGGTCAAGGC CTAGCTGGAT GCTCTCCTCG GGATTCTGGC 
TGCTATGAAA GCAGTGAAAA CCTGGAGAAT GGTAAAACCC GAAGGACCTG TCTTCTGCCC 
TCAAAATCAT CAAGTGAGCA TAGCTTTAAA GACTTCAGCA GACACCAGCT ATCAAATTAT 
CCCACACTCC CACTGACCAA GTCAATAGAA ACTCTACAGC AAGGAGAAAA AGAAAGCCGG 
TTGAGCTGTG CACATCGTGC TCTGAAGAGC TCTGTCAAGA CCCCAGCTGT TATGGGTCTG 
AAGAAGAACC GTAGAAGTTT ACCAGTTGCT GTCTGTCGGA GCTATGAAAC TCTGGATGGC 
CCCCAGAGTG TGGATGTATG GCCAAGATCT CACTCCCTGG ACGATCTCCA GGGAGATTCC 
GATACTAATC TACAAGATGG TAGCAAGAAA GATTATTTTG CTCGGGATTC ACTGGATACA 
GCGAAAAAGG CAACTGGATC TGCCCTGCCA CCTCAGTCGT GTGGGGGCAG TTGTATGGTC 
GATAACTTGA TGCCTAAGCA GGGTAAAGCA GCTGCTGGTT CTCAGAAGGG TAGAGGTAAG 
GAGTTGGAGT GCTCTGTAAT GGAGAGTGCA GTTGGGAAAC CTTTTCCATT CCCCATGAAA 
CACTGTGAAG CTCAGGCTGC CTTGGTGACA CATTCTGCAA CAAGGACACC TCTGGAAATA 
CAGAGTAAGG GCTTTCATGA CCTTGCACGG GCTGACTATG CTCCAGTCCT CAAGGGAGGT 
CTAGAAGCTG AGCAAAAAGG CACAAATGAG ACAAGAATGC AACCAAAAAA TCCCTCTCAG 
CCGCCACCTG TTCCTGCCAA AAAATGCCGA GAACGCCTTA CTAATGGATT ATATCATCCT 
CCCATGACCC CCAGTGGGAA CAACTCAAGT CAAGAAGCAC CGTGTTTGCC AGTAAAAAAG 
ACCAGTGCAT CCAATCCCAC TGATTTTCAT GGAGTACCTG TCCATAGGAC ATCTGAACAG 
GAGCCCAGTA CCCCTCCTAG CTCACTGCCA CCCTGGCTGT TGGAGCAACC AGAGACTAAA 
AGCGTTCAGC AGCACGTGAT AAAGCTAGGG CCTGCTTCAG CAAGGAAAGT CTCTTGTAGC 
AGGGGAATGG ATCTGGAAAT GGTAATAGAA AACAAGTTGC AGTCTGAAGG TATTGATCTT 
ACTGAAGAAC CATACTCAGA CAAGCATGGT CGCTGTGGCA TTCCTGAGGC TTTGGTACAG 
AGGTACTCCG AAGACTTAGA CCAGCCTGAA AAGGATGTTG CTGCAAACAT GGACCAAATC 
CGGGTAAAGC AGCTGAGGAA GCAGCATCGC ATGGCAATTC CAAGTGGAGG GCTCACTGAA 
ATTTGCCGAA AACCTGTATC ACCAGGACTT ATCACCTCCG TATCTGACTG GCTGATTTCC 
ATTGGCCTAC CCATGTATTC CAGCCCACTC ACAGAAGCAG GGTTCAACAC ACTGAGCAAA 
GTGCCTTCTC TGTCACAAAC TTGCCTTCAG GAAGCTGGCA TCACAGAGGA AAGGCACATA 
AGCAAGCTCC TGGCAGCAGC AAGACTCTTC AAACCTCTCG ATCCAGACGC AATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005139.1
CDS	131..4507
Translation	MCTNIVYEWLKTLQLPQYAESFVDNGYDDLEVCKQIGDPDLDAIGVAVPQHRRRIHEAVRRLREADERAAGLYFTLEPPPAPPAKGPRRPPGQDGAAPRHRPGRGGPVVYPRLKLKIMIRDKLVRDGINLSKPPYSNKDGSLGNIDDLAQQYADYYNTCFTDVCERMEELRKRRVSQDLDVEKPDASPTSLQLRSQIEESLGLSSTASTPDTERKLSIHKSSSEEGSVGKADWKKKNKFFWQNFRKNQKGLMRQTSKGEDVGYVASEITMSDEERIQLMMMVKEKMITIEEALARSREQSDDETEESVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGVEEQSLDNSAILDDRSALYSGVHKKQFYFDGSSEKQTEDDSDSLTTSPSSSSLDTWGASRKLVKTFSKTDSRGLIKPPKKLGTFFSYPEDEKTQKVCRSLTDGEMKKSLGSLSHGVSTESICFYDSNRHKHHLLVSLEEIQSVRKQIRLKKMDTNYSCSRAFLCQRPARKGASATTCDLQLLRHKVKGGGGCGFPNRRLRGRTSVSEFNITYVVERSLYSHLNLSQLVRPVTDRTLSKADKQDLRRCLLEEDEEAKRKWAATVDRCTKRVLLRIHQKSRTCSFGGFDLTNRSLHIGNNSDQMGKEGDFVYKEVIKSPSASRISLGKKVKSVKETMKKRMSKKYSSSLSEQESSPGVIPGSPQSPPPDTDSLDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRRKSRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVEHLQEYDSNSDQSGSQEKLLIEGQGLAGCSPRDSGCYESSENLENGKTRRTCLLPSKSSSEHSFKDFSRHQLSNYPTLPLTKSIETLQQGEKESRLSCAHRALKSSVKTPAVMGLKKNRRSLPVAVCRSYETLDGPQSVDVWPRSHSLDDLQGDSDTNLQDGSKKDYFARDSLDTAKKATGSALPPQSCGGSCMVDNLMPKQGKAAAGSQKGRGKELECSVMESAVGKPFPFPMKHCEAQAALVTHSATRTPLEIQSKGFHDLARADYAPVLKGGLEAEQKGTNETRMQPKNPSQPPPVPAKKCRERLTNGLYHPPMTPSGNNSSQEAPCLPVKKTSASNPTDFHGVPVHRTSEQEPSTPPSSLPPWLLEQPETKSVQQHVIKLGPASARKVSCSRGMDLEMVIENKLQSEGIDLTEEPYSDKHGRCGIPEALVQRYSEDLDQPEKDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGLITSVSDWLISIGLPMYSSPLTEAGFNTLSKVPSLSQTCLQEAGITEERHISKLLAAARLFKPLDPDAI

Target ORF information:

RefSeq Version	XM_025149371.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149371.1

ORF Insert Sequence:

ATGTGCACCA ACATAGTGTA CGAGTGGCTG AAAACCCTGC AGCTCCCCCA GTACGCCGAG 
TCCTTTGTCG ACAATGGCTA CGACGACCTG GAGGTCTGCA AGCAGATCGG CGACCCGGAC 
CTGGATGCCA TCGGCGTGGC CGTGCCCCAG CACCGCCGCC GCATCCACGA GGCCGTGCGG 
CGGCTGCGGG AGGCGGACGA GCGGGCGGCG GGGCTCTACT TCACCCTGGA GCCGCCGCCC 
GCGCCTCCCG CCAAGGGGCC CCGCCGCCCG CCGGGGCAGG ACGGGGCGGC CCCCCGGCAC 
CGGCCTGGCC GCGGGGGCCC GGTGGTCTAC CCCAGGCTGA AGCTGAAGAT CATGATCCGG 
GACAAGCTGG TCCGCGACGG CATCAACCTG AGCAAGCCCC CCTACTCCAA CAAGGATGGG 
TCTCTGGGAA ACATTGATGA CCTCGCACAG CAGTATGCAG ATTATTACAA CACCTGCTTC 
ACGGATGTGT GTGAGAGGAT GGAAGAGCTG CGCAAACGGA GGGTTTCCCA GGACCTTGAT 
GTGGAGAAAC CTGATGCCAG TCCCACATCT CTACAACTGC GATCCCAGAT TGAGGAGTCA 
CTTGGTCTCA GCAGCACCGC ATCAACACCT GACACTGAAA GAAAGCTCTC CATTCATAAA 
TCAAGTTCGG AAGAAGGCTC TGTAGGGAAA GCAGACTGGA AAAAGAAAAA TAAGTTTTTT 
TGGCAAAATT TCCGAAAGAA CCAGAAAGGA TTGATGAGGC AGACTTCAAA AGGAGAGGAT 
GTGGGGTATG TGGCCAGTGA GATTACGATG AGCGATGAGG AACGGATCCA GCTGATGATG 
ATGGTGAAAG AGAAGATGAT CACTATTGAG GAAGCACTTG CTAGGTCAAG AGAACAATCC 
GATGATGAAA CAGAGGAGTC GGTGAAGTTT AAGAGATTGC ACAAGCTGGT AAATTCTACT 
CGCAGAGTCA GGAAGAAACT CATAAGGGTG GAAGAAATGA AAAAACCCAG TACAGAAGGC 
GTAGAGGAGC AGTCGCTTGA CAACTCTGCT ATACTGGATG ACAGATCGGC ACTTTACTCT 
GGAGTTCACA AGAAGCAATT CTACTTTGAT GGTTCCTCCG AGAAGCAGAC AGAGGATGAC 
TCGGACTCAC TTACTACTTC TCCCTCATCT AGTAGTCTTG ATACATGGGG AGCTAGCCGG 
AAACTGGTGA AGACCTTCAG CAAAACTGAT AGCCGTGGCC TTATCAAGCC TCCCAAGAAA 
CTTGGGACAT TTTTTTCTTA CCCAGAAGAT GAGAAGACCC AGAAAGTTTG CCGCTCCTTG 
ACAGATGGGG AGATGAAGAA GAGCCTCGGC TCACTGAGCC ATGGGGTAAG TACAGAAAGC 
ATTTGCTTTT ATGACAGCAA CAGGCACAAG CACCATCTTC TGGTGTCCCT GGAGGAGATT 
CAGAGTGTAA GGAAGCAGAT CCGATTGAAG AAAATGGATA CTAACTATTC CTGTTCCAGA 
GCTTTTCTTT GCCAGCGCCC TGCTAGGAAA GGAGCATCCG CCACAACTTG CGACCTACAA 
TTACTGCGGC ACAAAGTGAA AGGGGGTGGA GGTTGTGGCT TCCCAAACAG AAGGCTACGA 
GGGCGCACTT CTGTCAGCGA GTTCAATATT ACCTATGTGG TGGAGAGGAG CCTGTACAGT 
CACCTCAACC TCTCACAGCT GGTGCGTCCC GTTACTGACA GGACCCTGAG CAAAGCTGAC 
AAGCAGGACC TGAGGAGATG CCTGCTAGAG GAGGATGAGG AGGCCAAGAG GAAGTGGGCT 
GCCACAGTGG ATCGTTGTAC TAAAAGGGTT CTCTTGAGAA TCCACCAAAA GTCTAGAACC 
TGCAGCTTTG GAGGATTTGA CTTGACAAAT CGTTCCCTTC ATATTGGAAA CAATTCTGAC 
CAAATGGGCA AAGAAGGTGA CTTTGTTTAT AAAGAAGTAA TCAAATCACC CTCTGCCTCC 
CGTATATCTC TGGGCAAAAA GGTGAAATCT GTGAAAGAAA CCATGAAGAA AAGGATGTCT 
AAAAAATACA GCAGCTCTCT GTCTGAGCAG GAGTCGAGCC CTGGAGTCAT ACCGGGTTCT 
CCGCAGTCGC CGCCGCCAGA CACTGACTCT CTGGACAAGC CCAAGCTGAA AGCTGGTGGC 
TCAGTTGAGA GCCTGAGGAG TTCCTTAAGT GGTCAGAGCT CAATGAGTGG TCAGACAGTG 
AGTACAACAG ATTCCTCAAC CAGCAACAGG GAGAGCGTGA AATCAGAAGA TGGGGATGAT 
GAAGAACCTC CCTACAGAGG ACCCTTCTGT GGAAGAGCCA GGGTTCACAC TGATTTTACT 
CCAAGTCCTT ATGATACAGA CTCTCTGAAG CTCAAGAAAG GTGATATCAT TGACATCATC 
AGCAAACCCC CCATGGGCAC TTGGATGGGC CTATTGAACA ACAAAGTTGG CACTTTCAAA 
TTTATCTATG TGGATGTGTT AAATGAAGAG GAAGAGAAGC CGAAGCGACC CACCAGGAGA 
CGCCGAAAAA GCAGACCACC CCAACCCAAG TCAGTTGAGG ATCTCTTGGA TCGCATCAAC 
TTAAAGGAGC ACATGCCCAC TTTTCTGTTC AATGGTTATG AAGATCTGGA TACCTTTAAG 
CTTTTAGAAG AAGAGGATTT GGATGAACTG AACATCCGAG ATCCTGAGCA CAGAGCTGTT 
CTCCTCACAG CCGTGGAACA TCTACAGGAG TATGATAGTA ACAGCGACCA GTCAGGATCT 
CAGGAGAAAC TTCTCATTGA AGGTCAAGGC CTAGCTGGAT GCTCTCCTCG GGATTCTGGC 
TGCTATGAAA GCAGTGAAAA CCTGGAGAAT GGTAAAACCC GAAGGACCTG TCTTCTGCCC 
TCAAAATCAT CAAGTGAGCA TAGCTTTAAA GACTTCAGCA GACACCAGCT ATCAAATTAT 
CCCACACTCC CACTGACCAA GTCAATAGAA ACTCTACAGC AAGGAGAAAA AGAAAGCCGG 
TTGAGCTGTG CACATCGTGC TCTGAAGAGC TCTGTCAAGA CCCCAGCTGT TATGGGTCTG 
AAGAAGAACC GTAGAAGTTT ACCAGTTGCT GTCTGTCGGA GCTATGAAAC TCTGGATGGC 
CCCCAGAGTG TGGATGTATG GCCAAGATCT CACTCCCTGG ACGATCTCCA GGGAGATTCC 
GATACTAATC TACAAGATGG TAGCAAGAAA GATTATTTTG CTCGGGATTC ACTGGATACA 
GCGAAAAAGG CAACTGGATC TGCCCTGCCA CCTCAGTCGT GTGGGGGCAG TTGTATGGTC 
GATAACTTGA TGCCTAAGCA GGGTAAAGCA GCTGCTGGTT CTCAGAAGGG TAGAGGTAAG 
GAGTTGGAGT GCTCTGTAAT GGAGAGTGCA GTTGGGAAAC CTTTTCCATT CCCCATGAAA 
CACTGTGAAG CTCAGGCTGC CTTGGTGACA CATTCTGCAA CAAGGACACC TCTGGAAATA 
CAGAGTAAGG GCTTTCATGA CCTTGCACGG GCTGACTATG CTCCAGTCCT CAAGGGAGGT 
CTAGAAGCTG AGCAAAAAGG CACAAATGAG ACAAGAATGC AACCAAAAAA TCCCTCTCAG 
CCGCCACCTG TTCCTGCCAA AAAATGCCGA GAACGCCTTA CTAATGGATT ATATCATCCT 
CCCATGACCC CCAGTGGGAA CAACTCAAGT CAAGAAGCAC CGTGTTTGCC AGTAAAAAAG 
ACCAGTGCAT CCAATCCCAC TGATTTTCAT GGAGTACCTG TCCATAGGAC ATCTGAACAG 
GAGCCCAGTA CCCCTCCTAG CTCACTGCCA CCCTGGCTGT TGGAGCAACC AGAGACTAAA 
AGCGTTCAGC AGCACGTGAT AAAGCTAGGG CCTGCTTCAG CAAGGAAAGT CTCTTGTAGC 
AGGGGAATGG ATCTGGAAAT GGTAATAGAA AACAAGTTGC AGTCTGAAGG TATTGATCTT 
ACTGAAGAAC CATACTCAGA CAAGCATGGT CGCTGTGGCA TTCCTGAGGC TTTGGTACAG 
AGGTACTCCG AAGACTTAGA CCAGCCTGAA AAGGATGTTG CTGCAAACAT GGACCAAATC 
CGGGTAAAGC AGCTGAGGAA GCAGCATCGC ATGGCAATTC CAAGTGGAGG GCTCACTGAA 
ATTTGCCGAA AACCTGTATC ACCAGGACTT ATCACCTCCG TATCTGACTG GCTGATTTCC 
ATTGGCCTAC CCATGTATTC CAGCCCACTC ACAGAAGCAG GGTTCAACAC ACTGAGCAAA 
GTGCCTTCTC TGTCACAAAC TTGCCTTCAG GAAGCTGGCA TCACAGAGGA AAGGCACATA 
AGCAAGCTCC TGGCAGCAGC AAGACTCTTC AAACCTCTCG ATCCAGACGC AATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH1 ( XM_025149373.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005141.1 Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X5, mRNA.

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CloneID	OGa149134
Clone ID Related Accession (Same CDS sequence)	XM_025149373.1
Accession Version	XM_025149373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	SAM and SH3 domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC TGCGCAAACG GAGGGTTTCC CAGGACCTTG ATGTGGAGAA ACCTGATGCC 
AGTCCCACAT CTCTACAACT GCGATCCCAG ATTGAGGAGT CACTTGGTCT CAGCAGCACC 
GCATCAACAC CTGACACTGA AAGAAAGCTC TCCATTCATA AATCAAGTTC GGAAGAAGGC 
TCTGTAGGGA AAGCAGACTG GAAAAAGAAA AATAAGTTTT TTTGGCAAAA TTTCCGAAAG 
AACCAGAAAG GATTGATGAG GCAGACTTCA AAAGGAGAGG ATGTGGGGTA TGTGGCCAGT 
GAGATTACGA TGAGCGATGA GGAACGGATC CAGCTGATGA TGATGGTGAA AGAGAAGATG 
ATCACTATTG AGGAAGCACT TGCTAGGCTG AAGGAGTATG AGTCCCAGCA CAGGCAATCA 
AGTACTGTAG ATGCTACAGA CTGGCCTGAT GGTTCTTACT CAACATTTGA TGGGTCTTCC 
AATTGTAATT CAAGAGAACA ATCCGATGAT GAAACAGAGG AGTCGGTGAA GTTTAAGAGA 
TTGCACAAGC TGGTAAATTC TACTCGCAGA GTCAGGAAGA AACTCATAAG GGTGGAAGAA 
ATGAAAAAAC CCAGTACAGA AGGCGTAGAG GAGCAGTCGC TTGACAACTC TGCTATACTG 
GATGACAGAT CGGCACTTTA CTCTGGAGTT CACAAGAAGC AATTCTACTT TGATGGTTCC 
TCCGAGAAGC AGACAGAGGA TGACTCGGAC TCACTTACTA CTTCTCCCTC ATCTAGTAGT 
CTTGATACAT GGGGAGCTAG CCGGAAACTG GTGAAGACCT TCAGCAAAAC TGATAGCCGT 
GGCCTTATCA AGCCTCCCAA GAAACTTGGG ACATTTTTTT CTTACCCAGA AGATGAGAAG 
ACCCAGAAAG TTTGCCGCTC CTTGACAGAT GGGGAGATGA AGAAGAGCCT CGGCTCACTG 
AGCCATGGGG TAAGTACAGA AAGCATTTGC TTTTATGACA GCAACAGGCA CAAGCACCAT 
CTTCTGGTGT CCCTGGAGGA GATTCAGAGT GTAAGGAAGC AGATCCGATT GAAGAAAATG 
GATACTAACT ATTCCTGTTC CAGAGCTTTT CTTTGCCAGC GCCCTGCTAG GAAAGGAGCA 
TCCGCCACAA CTTGCGACCT ACAATTACTG CGGCACAAAG TGAAAGGGGG TGGAGGTTGT 
GGCTTCCCAA ACAGAAGGCT ACGAGGGCGC ACTTCTGTCA GCGAGTTCAA TATTACCTAT 
GTGGTGGAGA GGAGCCTGTA CAGTCACCTC AACCTCTCAC AGCTGGTGCG TCCCGTTACT 
GACAGGACCC TGAGCAAAGC TGACAAGCAG GACCTGAGGA GATGCCTGCT AGAGGAGGAT 
GAGGAGGCCA AGAGGAAGTG GGCTGCCACA GTGGATCGTT GTACTAAAAG GGTTCTCTTG 
AGAATCCACC AAAAGTCTAG AACCTGCAGC TTTGGAGGAT TTGACTTGAC AAATCGTTCC 
CTTCATATTG GAAACAATTC TGACCAAATG GGCAAAGAAG GTGACTTTGT TTATAAAGAA 
GTAATCAAAT CACCCTCTGC CTCCCGTATA TCTCTGGGCA AAAAGGTGAA ATCTGTGAAA 
GAAACCATGA AGAAAAGGAT GTCTAAAAAA TACAGCAGCT CTCTGTCTGA GCAGGAGTCG 
AGCCCTGGAG TCATACCGGG TTCTCCGCAG TCGCCGCCGC CAGACACTGA CTCTCTGGAC 
AAGCCCAAGC TGAAAGCTGG TGGCTCAGTT GAGAGCCTGA GGAGTTCCTT AAGTGGTCAG 
AGCTCAATGA GTGGTCAGAC AGTGAGTACA ACAGATTCCT CAACCAGCAA CAGGGAGAGC 
GTGAAATCAG AAGATGGGGA TGATGAAGAA CCTCCCTACA GAGGACCCTT CTGTGGAAGA 
GCCAGGGTTC ACACTGATTT TACTCCAAGT CCTTATGATA CAGACTCTCT GAAGCTCAAG 
AAAGGTGATA TCATTGACAT CATCAGCAAA CCCCCCATGG GCACTTGGAT GGGCCTATTG 
AACAACAAAG TTGGCACTTT CAAATTTATC TATGTGGATG TGTTAAATGA AGAGGAAGAG 
AAGCCGAAGC GACCCACCAG GAGACGCCGA AAAAGCAGAC CACCCCAACC CAAGTCAGTT 
GAGGATCTCT TGGATCGCAT CAACTTAAAG GAGCACATGC CCACTTTTCT GTTCAATGGT 
TATGAAGATC TGGATACCTT TAAGCTTTTA GAAGAAGAGG ATTTGGATGA ACTGAACATC 
CGAGATCCTG AGCACAGAGC TGTTCTCCTC ACAGCCGTGG AACATCTACA GGAGTATGAT 
AGTAACAGCG ACCAGTCAGG ATCTCAGGAG AAACTTCTCA TTGAAGGTCA AGGCCTAGCT 
GGATGCTCTC CTCGGGATTC TGGCTGCTAT GAAAGCAGTG AAAACCTGGA GAATGGTAAA 
ACCCGAAGGA CCTGTCTTCT GCCCTCAAAA TCATCAAGTG AGCATAGCTT TAAAGACTTC 
AGCAGACACC AGCTATCAAA TTATCCCACA CTCCCACTGA CCAAGTCAAT AGAAACTCTA 
CAGCAAGGAG AAAAAGAAAG CCGGTTGAGC TGTGCACATC GTGCTCTGAA GAGCTCTGTC 
AAGACCCCAG CTGTTATGGG TCTGAAGAAG AACCGTAGAA GTTTACCAGT TGCTGTCTGT 
CGGAGCTATG AAACTCTGGA TGGCCCCCAG AGTGTGGATG TATGGCCAAG ATCTCACTCC 
CTGGACGATC TCCAGGGAGA TTCCGATACT AATCTACAAG ATGGTAGCAA GAAAGATTAT 
TTTGCTCGGG ATTCACTGGA TACAGCGAAA AAGGCAACTG GATCTGCCCT GCCACCTCAG 
TCGTGTGGGG GCAGTTGTAT GGTCGATAAC TTGATGCCTA AGCAGGGTAA AGCAGCTGCT 
GGTTCTCAGA AGGGTAGAGG TAAGGAGTTG GAGTGCTCTG TAATGGAGAG TGCAGTTGGG 
AAACCTTTTC CATTCCCCAT GAAACACTGT GAAGCTCAGG CTGCCTTGGT GACACATTCT 
GCAACAAGGA CACCTCTGGA AATACAGAGT AAGGGCTTTC ATGACCTTGC ACGGGCTGAC 
TATGCTCCAG TCCTCAAGGG AGGTCTAGAA GCTGAGCAAA AAGGCACAAA TGAGACAAGA 
ATGCAACCAA AAAATCCCTC TCAGCCGCCA CCTGTTCCTG CCAAAAAATG CCGAGAACGC 
CTTACTAATG GATTATATCA TCCTCCCATG ACCCCCAGTG GGAACAACTC AAGTCAAGAA 
GCACCGTGTT TGCCAGTAAA AAAGACCAGT GCATCCAATC CCACTGATTT TCATGGAGTA 
CCTGTCCATA GGACATCTGA ACAGGAGCCC AGTACCCCTC CTAGCTCACT GCCACCCTGG 
CTGTTGGAGC AACCAGAGAC TAAAAGCGTT CAGCAGCACG TGATAAAGCT AGGGCCTGCT 
TCAGCAAGGA AAGTCTCTTG TAGCAGGGGA ATGGATCTGG AAATGGTAAT AGAAAACAAG 
TTGCAGTCTG AAGGTATTGA TCTTACTGAA GAACCATACT CAGACAAGCA TGGTCGCTGT 
GGCATTCCTG AGGCTTTGGT ACAGAGGTAC TCCGAAGACT TAGACCAGCC TGAAAAGGAT 
GTTGCTGCAA ACATGGACCA AATCCGGGTA AAGCAGCTGA GGAAGCAGCA TCGCATGGCA 
ATTCCAAGTG GAGGGCTCAC TGAAATTTGC CGAAAACCTG TATCACCAGG ACTTATCACC 
TCCGTATCTG ACTGGCTGAT TTCCATTGGC CTACCCATGT ATTCCAGCCC ACTCACAGAA 
GCAGGGTTCA ACACACTGAG CAAAGTGCCT TCTCTGTCAC AAACTTGCCT TCAGGAAGCT 
GGCATCACAG AGGAAAGGCA CATAAGCAAG CTCCTGGCAG CAGCAAGACT CTTCAAACCT 
CTCGATCCAG ACGCAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005141.1
CDS	249..4229
Translation	MEELRKRRVSQDLDVEKPDASPTSLQLRSQIEESLGLSSTASTPDTERKLSIHKSSSEEGSVGKADWKKKNKFFWQNFRKNQKGLMRQTSKGEDVGYVASEITMSDEERIQLMMMVKEKMITIEEALARLKEYESQHRQSSTVDATDWPDGSYSTFDGSSNCNSREQSDDETEESVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGVEEQSLDNSAILDDRSALYSGVHKKQFYFDGSSEKQTEDDSDSLTTSPSSSSLDTWGASRKLVKTFSKTDSRGLIKPPKKLGTFFSYPEDEKTQKVCRSLTDGEMKKSLGSLSHGVSTESICFYDSNRHKHHLLVSLEEIQSVRKQIRLKKMDTNYSCSRAFLCQRPARKGASATTCDLQLLRHKVKGGGGCGFPNRRLRGRTSVSEFNITYVVERSLYSHLNLSQLVRPVTDRTLSKADKQDLRRCLLEEDEEAKRKWAATVDRCTKRVLLRIHQKSRTCSFGGFDLTNRSLHIGNNSDQMGKEGDFVYKEVIKSPSASRISLGKKVKSVKETMKKRMSKKYSSSLSEQESSPGVIPGSPQSPPPDTDSLDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRRKSRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVEHLQEYDSNSDQSGSQEKLLIEGQGLAGCSPRDSGCYESSENLENGKTRRTCLLPSKSSSEHSFKDFSRHQLSNYPTLPLTKSIETLQQGEKESRLSCAHRALKSSVKTPAVMGLKKNRRSLPVAVCRSYETLDGPQSVDVWPRSHSLDDLQGDSDTNLQDGSKKDYFARDSLDTAKKATGSALPPQSCGGSCMVDNLMPKQGKAAAGSQKGRGKELECSVMESAVGKPFPFPMKHCEAQAALVTHSATRTPLEIQSKGFHDLARADYAPVLKGGLEAEQKGTNETRMQPKNPSQPPPVPAKKCRERLTNGLYHPPMTPSGNNSSQEAPCLPVKKTSASNPTDFHGVPVHRTSEQEPSTPPSSLPPWLLEQPETKSVQQHVIKLGPASARKVSCSRGMDLEMVIENKLQSEGIDLTEEPYSDKHGRCGIPEALVQRYSEDLDQPEKDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGLITSVSDWLISIGLPMYSSPLTEAGFNTLSKVPSLSQTCLQEAGITEERHISKLLAAARLFKPLDPDAI

Target ORF information:

RefSeq Version	XM_025149373.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149373.1

ORF Insert Sequence:

ATGGAAGAGC TGCGCAAACG GAGGGTTTCC CAGGACCTTG ATGTGGAGAA ACCTGATGCC 
AGTCCCACAT CTCTACAACT GCGATCCCAG ATTGAGGAGT CACTTGGTCT CAGCAGCACC 
GCATCAACAC CTGACACTGA AAGAAAGCTC TCCATTCATA AATCAAGTTC GGAAGAAGGC 
TCTGTAGGGA AAGCAGACTG GAAAAAGAAA AATAAGTTTT TTTGGCAAAA TTTCCGAAAG 
AACCAGAAAG GATTGATGAG GCAGACTTCA AAAGGAGAGG ATGTGGGGTA TGTGGCCAGT 
GAGATTACGA TGAGCGATGA GGAACGGATC CAGCTGATGA TGATGGTGAA AGAGAAGATG 
ATCACTATTG AGGAAGCACT TGCTAGGCTG AAGGAGTATG AGTCCCAGCA CAGGCAATCA 
AGTACTGTAG ATGCTACAGA CTGGCCTGAT GGTTCTTACT CAACATTTGA TGGGTCTTCC 
AATTGTAATT CAAGAGAACA ATCCGATGAT GAAACAGAGG AGTCGGTGAA GTTTAAGAGA 
TTGCACAAGC TGGTAAATTC TACTCGCAGA GTCAGGAAGA AACTCATAAG GGTGGAAGAA 
ATGAAAAAAC CCAGTACAGA AGGCGTAGAG GAGCAGTCGC TTGACAACTC TGCTATACTG 
GATGACAGAT CGGCACTTTA CTCTGGAGTT CACAAGAAGC AATTCTACTT TGATGGTTCC 
TCCGAGAAGC AGACAGAGGA TGACTCGGAC TCACTTACTA CTTCTCCCTC ATCTAGTAGT 
CTTGATACAT GGGGAGCTAG CCGGAAACTG GTGAAGACCT TCAGCAAAAC TGATAGCCGT 
GGCCTTATCA AGCCTCCCAA GAAACTTGGG ACATTTTTTT CTTACCCAGA AGATGAGAAG 
ACCCAGAAAG TTTGCCGCTC CTTGACAGAT GGGGAGATGA AGAAGAGCCT CGGCTCACTG 
AGCCATGGGG TAAGTACAGA AAGCATTTGC TTTTATGACA GCAACAGGCA CAAGCACCAT 
CTTCTGGTGT CCCTGGAGGA GATTCAGAGT GTAAGGAAGC AGATCCGATT GAAGAAAATG 
GATACTAACT ATTCCTGTTC CAGAGCTTTT CTTTGCCAGC GCCCTGCTAG GAAAGGAGCA 
TCCGCCACAA CTTGCGACCT ACAATTACTG CGGCACAAAG TGAAAGGGGG TGGAGGTTGT 
GGCTTCCCAA ACAGAAGGCT ACGAGGGCGC ACTTCTGTCA GCGAGTTCAA TATTACCTAT 
GTGGTGGAGA GGAGCCTGTA CAGTCACCTC AACCTCTCAC AGCTGGTGCG TCCCGTTACT 
GACAGGACCC TGAGCAAAGC TGACAAGCAG GACCTGAGGA GATGCCTGCT AGAGGAGGAT 
GAGGAGGCCA AGAGGAAGTG GGCTGCCACA GTGGATCGTT GTACTAAAAG GGTTCTCTTG 
AGAATCCACC AAAAGTCTAG AACCTGCAGC TTTGGAGGAT TTGACTTGAC AAATCGTTCC 
CTTCATATTG GAAACAATTC TGACCAAATG GGCAAAGAAG GTGACTTTGT TTATAAAGAA 
GTAATCAAAT CACCCTCTGC CTCCCGTATA TCTCTGGGCA AAAAGGTGAA ATCTGTGAAA 
GAAACCATGA AGAAAAGGAT GTCTAAAAAA TACAGCAGCT CTCTGTCTGA GCAGGAGTCG 
AGCCCTGGAG TCATACCGGG TTCTCCGCAG TCGCCGCCGC CAGACACTGA CTCTCTGGAC 
AAGCCCAAGC TGAAAGCTGG TGGCTCAGTT GAGAGCCTGA GGAGTTCCTT AAGTGGTCAG 
AGCTCAATGA GTGGTCAGAC AGTGAGTACA ACAGATTCCT CAACCAGCAA CAGGGAGAGC 
GTGAAATCAG AAGATGGGGA TGATGAAGAA CCTCCCTACA GAGGACCCTT CTGTGGAAGA 
GCCAGGGTTC ACACTGATTT TACTCCAAGT CCTTATGATA CAGACTCTCT GAAGCTCAAG 
AAAGGTGATA TCATTGACAT CATCAGCAAA CCCCCCATGG GCACTTGGAT GGGCCTATTG 
AACAACAAAG TTGGCACTTT CAAATTTATC TATGTGGATG TGTTAAATGA AGAGGAAGAG 
AAGCCGAAGC GACCCACCAG GAGACGCCGA AAAAGCAGAC CACCCCAACC CAAGTCAGTT 
GAGGATCTCT TGGATCGCAT CAACTTAAAG GAGCACATGC CCACTTTTCT GTTCAATGGT 
TATGAAGATC TGGATACCTT TAAGCTTTTA GAAGAAGAGG ATTTGGATGA ACTGAACATC 
CGAGATCCTG AGCACAGAGC TGTTCTCCTC ACAGCCGTGG AACATCTACA GGAGTATGAT 
AGTAACAGCG ACCAGTCAGG ATCTCAGGAG AAACTTCTCA TTGAAGGTCA AGGCCTAGCT 
GGATGCTCTC CTCGGGATTC TGGCTGCTAT GAAAGCAGTG AAAACCTGGA GAATGGTAAA 
ACCCGAAGGA CCTGTCTTCT GCCCTCAAAA TCATCAAGTG AGCATAGCTT TAAAGACTTC 
AGCAGACACC AGCTATCAAA TTATCCCACA CTCCCACTGA CCAAGTCAAT AGAAACTCTA 
CAGCAAGGAG AAAAAGAAAG CCGGTTGAGC TGTGCACATC GTGCTCTGAA GAGCTCTGTC 
AAGACCCCAG CTGTTATGGG TCTGAAGAAG AACCGTAGAA GTTTACCAGT TGCTGTCTGT 
CGGAGCTATG AAACTCTGGA TGGCCCCCAG AGTGTGGATG TATGGCCAAG ATCTCACTCC 
CTGGACGATC TCCAGGGAGA TTCCGATACT AATCTACAAG ATGGTAGCAA GAAAGATTAT 
TTTGCTCGGG ATTCACTGGA TACAGCGAAA AAGGCAACTG GATCTGCCCT GCCACCTCAG 
TCGTGTGGGG GCAGTTGTAT GGTCGATAAC TTGATGCCTA AGCAGGGTAA AGCAGCTGCT 
GGTTCTCAGA AGGGTAGAGG TAAGGAGTTG GAGTGCTCTG TAATGGAGAG TGCAGTTGGG 
AAACCTTTTC CATTCCCCAT GAAACACTGT GAAGCTCAGG CTGCCTTGGT GACACATTCT 
GCAACAAGGA CACCTCTGGA AATACAGAGT AAGGGCTTTC ATGACCTTGC ACGGGCTGAC 
TATGCTCCAG TCCTCAAGGG AGGTCTAGAA GCTGAGCAAA AAGGCACAAA TGAGACAAGA 
ATGCAACCAA AAAATCCCTC TCAGCCGCCA CCTGTTCCTG CCAAAAAATG CCGAGAACGC 
CTTACTAATG GATTATATCA TCCTCCCATG ACCCCCAGTG GGAACAACTC AAGTCAAGAA 
GCACCGTGTT TGCCAGTAAA AAAGACCAGT GCATCCAATC CCACTGATTT TCATGGAGTA 
CCTGTCCATA GGACATCTGA ACAGGAGCCC AGTACCCCTC CTAGCTCACT GCCACCCTGG 
CTGTTGGAGC AACCAGAGAC TAAAAGCGTT CAGCAGCACG TGATAAAGCT AGGGCCTGCT 
TCAGCAAGGA AAGTCTCTTG TAGCAGGGGA ATGGATCTGG AAATGGTAAT AGAAAACAAG 
TTGCAGTCTG AAGGTATTGA TCTTACTGAA GAACCATACT CAGACAAGCA TGGTCGCTGT 
GGCATTCCTG AGGCTTTGGT ACAGAGGTAC TCCGAAGACT TAGACCAGCC TGAAAAGGAT 
GTTGCTGCAA ACATGGACCA AATCCGGGTA AAGCAGCTGA GGAAGCAGCA TCGCATGGCA 
ATTCCAAGTG GAGGGCTCAC TGAAATTTGC CGAAAACCTG TATCACCAGG ACTTATCACC 
TCCGTATCTG ACTGGCTGAT TTCCATTGGC CTACCCATGT ATTCCAGCCC ACTCACAGAA 
GCAGGGTTCA ACACACTGAG CAAAGTGCCT TCTCTGTCAC AAACTTGCCT TCAGGAAGCT 
GGCATCACAG AGGAAAGGCA CATAAGCAAG CTCCTGGCAG CAGCAAGACT CTTCAAACCT 
CTCGATCCAG ACGCAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH1 ( XM_025149374.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005142.1 Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OGa149135
Clone ID Related Accession (Same CDS sequence)	XM_025149374.1
Accession Version	XM_025149374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	SAM and SH3 domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTAGAA TCTTACGTTG CACAGATTTA GAAATCAAAA TGTCAAGAGA ACAATCCGAT 
GATGAAACAG AGGAGTCGGT GAAGTTTAAG AGATTGCACA AGCTGGTAAA TTCTACTCGC 
AGAGTCAGGA AGAAACTCAT AAGGGTGGAA GAAATGAAAA AACCCAGTAC AGAAGGCGTA 
GAGGAGCAGT CGCTTGACAA CTCTGCTATA CTGGATGACA GATCGGCACT TTACTCTGGA 
GTTCACAAGA AGCAATTCTA CTTTGATGGT TCCTCCGAGA AGCAGACAGA GGATGACTCG 
GACTCACTTA CTACTTCTCC CTCATCTAGT AGTCTTGATA CATGGGGAGC TAGCCGGAAA 
CTGGTGAAGA CCTTCAGCAA AACTGATAGC CGTGGCCTTA TCAAGCCTCC CAAGAAACTT 
GGGACATTTT TTTCTTACCC AGAAGATGAG AAGACCCAGA AAGTTTGCCG CTCCTTGACA 
GATGGGGAGA TGAAGAAGAG CCTCGGCTCA CTGAGCCATG GGGTAAGTAC AGAAAGCATT 
TGCTTTTATG ACAGCAACAG GCACAAGCAC CATCTTCTGG TGTCCCTGGA GGAGATTCAG 
AGTGTAAGGA AGCAGATCCG ATTGAAGAAA ATGGATACTA ACTATTCCTG TTCCAGAGCT 
TTTCTTTGCC AGCGCCCTGC TAGGAAAGGA GCATCCGCCA CAACTTGCGA CCTACAATTA 
CTGCGGCACA AAGTGAAAGG GGGTGGAGGT TGTGGCTTCC CAAACAGAAG GCTACGAGGG 
CGCACTTCTG TCAGCGAGTT CAATATTACC TATGTGGTGG AGAGGAGCCT GTACAGTCAC 
CTCAACCTCT CACAGCTGGT GCGTCCCGTT ACTGACAGGA CCCTGAGCAA AGCTGACAAG 
CAGGACCTGA GGAGATGCCT GCTAGAGGAG GATGAGGAGG CCAAGAGGAA GTGGGCTGCC 
ACAGTGGATC GTTGTACTAA AAGGGTTCTC TTGAGAATCC ACCAAAAGTC TAGAACCTGC 
AGCTTTGGAG GATTTGACTT GACAAATCGT TCCCTTCATA TTGGAAACAA TTCTGACCAA 
ATGGGCAAAG AAGGTGACTT TGTTTATAAA GAAGTAATCA AATCACCCTC TGCCTCCCGT 
ATATCTCTGG GCAAAAAGGT GAAATCTGTG AAAGAAACCA TGAAGAAAAG GATGTCTAAA 
AAATACAGCA GCTCTCTGTC TGAGCAGGAG TCGAGCCCTG GAGTCATACC GGGTTCTCCG 
CAGTCGCCGC CGCCAGACAC TGACTCTCTG GACAAGCCCA AGCTGAAAGC TGGTGGCTCA 
GTTGAGAGCC TGAGGAGTTC CTTAAGTGGT CAGAGCTCAA TGAGTGGTCA GACAGTGAGT 
ACAACAGATT CCTCAACCAG CAACAGGGAG AGCGTGAAAT CAGAAGATGG GGATGATGAA 
GAACCTCCCT ACAGAGGACC CTTCTGTGGA AGAGCCAGGG TTCACACTGA TTTTACTCCA 
AGTCCTTATG ATACAGACTC TCTGAAGCTC AAGAAAGGTG ATATCATTGA CATCATCAGC 
AAACCCCCCA TGGGCACTTG GATGGGCCTA TTGAACAACA AAGTTGGCAC TTTCAAATTT 
ATCTATGTGG ATGTGTTAAA TGAAGAGGAA GAGAAGCCGA AGCGACCCAC CAGGAGACGC 
CGAAAAAGCA GACCACCCCA ACCCAAGTCA GTTGAGGATC TCTTGGATCG CATCAACTTA 
AAGGAGCACA TGCCCACTTT TCTGTTCAAT GGTTATGAAG ATCTGGATAC CTTTAAGCTT 
TTAGAAGAAG AGGATTTGGA TGAACTGAAC ATCCGAGATC CTGAGCACAG AGCTGTTCTC 
CTCACAGCCG TGGAACATCT ACAGGAGTAT GATAGTAACA GCGACCAGTC AGGATCTCAG 
GAGAAACTTC TCATTGAAGG TCAAGGCCTA GCTGGATGCT CTCCTCGGGA TTCTGGCTGC 
TATGAAAGCA GTGAAAACCT GGAGAATGGT AAAACCCGAA GGACCTGTCT TCTGCCCTCA 
AAATCATCAA GTGAGCATAG CTTTAAAGAC TTCAGCAGAC ACCAGCTATC AAATTATCCC 
ACACTCCCAC TGACCAAGTC AATAGAAACT CTACAGCAAG GAGAAAAAGA AAGCCGGTTG 
AGCTGTGCAC ATCGTGCTCT GAAGAGCTCT GTCAAGACCC CAGCTGTTAT GGGTCTGAAG 
AAGAACCGTA GAAGTTTACC AGTTGCTGTC TGTCGGAGCT ATGAAACTCT GGATGGCCCC 
CAGAGTGTGG ATGTATGGCC AAGATCTCAC TCCCTGGACG ATCTCCAGGG AGATTCCGAT 
ACTAATCTAC AAGATGGTAG CAAGAAAGAT TATTTTGCTC GGGATTCACT GGATACAGCG 
AAAAAGGCAA CTGGATCTGC CCTGCCACCT CAGTCGTGTG GGGGCAGTTG TATGGTCGAT 
AACTTGATGC CTAAGCAGGG TAAAGCAGCT GCTGGTTCTC AGAAGGGTAG AGGTAAGGAG 
TTGGAGTGCT CTGTAATGGA GAGTGCAGTT GGGAAACCTT TTCCATTCCC CATGAAACAC 
TGTGAAGCTC AGGCTGCCTT GGTGACACAT TCTGCAACAA GGACACCTCT GGAAATACAG 
AGTAAGGGCT TTCATGACCT TGCACGGGCT GACTATGCTC CAGTCCTCAA GGGAGGTCTA 
GAAGCTGAGC AAAAAGGCAC AAATGAGACA AGAATGCAAC CAAAAAATCC CTCTCAGCCG 
CCACCTGTTC CTGCCAAAAA ATGCCGAGAA CGCCTTACTA ATGGATTATA TCATCCTCCC 
ATGACCCCCA GTGGGAACAA CTCAAGTCAA GAAGCACCGT GTTTGCCAGT AAAAAAGACC 
AGTGCATCCA ATCCCACTGA TTTTCATGGA GTACCTGTCC ATAGGACATC TGAACAGGAG 
CCCAGTACCC CTCCTAGCTC ACTGCCACCC TGGCTGTTGG AGCAACCAGA GACTAAAAGC 
GTTCAGCAGC ACGTGATAAA GCTAGGGCCT GCTTCAGCAA GGAAAGTCTC TTGTAGCAGG 
GGAATGGATC TGGAAATGGT AATAGAAAAC AAGTTGCAGT CTGAAGGTAT TGATCTTACT 
GAAGAACCAT ACTCAGACAA GCATGGTCGC TGTGGCATTC CTGAGGCTTT GGTACAGAGG 
TACTCCGAAG ACTTAGACCA GCCTGAAAAG GATGTTGCTG CAAACATGGA CCAAATCCGG 
GTAAAGCAGC TGAGGAAGCA GCATCGCATG GCAATTCCAA GTGGAGGGCT CACTGAAATT 
TGCCGAAAAC CTGTATCACC AGGACTTATC ACCTCCGTAT CTGACTGGCT GATTTCCATT 
GGCCTACCCA TGTATTCCAG CCCACTCACA GAAGCAGGGT TCAACACACT GAGCAAAGTG 
CCTTCTCTGT CACAAACTTG CCTTCAGGAA GCTGGCATCA CAGAGGAAAG GCACATAAGC 
AAGCTCCTGG CAGCAGCAAG ACTCTTCAAA CCTCTCGATC CAGACGCAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005142.1
CDS	412..3945
Translation	MPRILRCTDLEIKMSREQSDDETEESVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGVEEQSLDNSAILDDRSALYSGVHKKQFYFDGSSEKQTEDDSDSLTTSPSSSSLDTWGASRKLVKTFSKTDSRGLIKPPKKLGTFFSYPEDEKTQKVCRSLTDGEMKKSLGSLSHGVSTESICFYDSNRHKHHLLVSLEEIQSVRKQIRLKKMDTNYSCSRAFLCQRPARKGASATTCDLQLLRHKVKGGGGCGFPNRRLRGRTSVSEFNITYVVERSLYSHLNLSQLVRPVTDRTLSKADKQDLRRCLLEEDEEAKRKWAATVDRCTKRVLLRIHQKSRTCSFGGFDLTNRSLHIGNNSDQMGKEGDFVYKEVIKSPSASRISLGKKVKSVKETMKKRMSKKYSSSLSEQESSPGVIPGSPQSPPPDTDSLDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRRKSRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVEHLQEYDSNSDQSGSQEKLLIEGQGLAGCSPRDSGCYESSENLENGKTRRTCLLPSKSSSEHSFKDFSRHQLSNYPTLPLTKSIETLQQGEKESRLSCAHRALKSSVKTPAVMGLKKNRRSLPVAVCRSYETLDGPQSVDVWPRSHSLDDLQGDSDTNLQDGSKKDYFARDSLDTAKKATGSALPPQSCGGSCMVDNLMPKQGKAAAGSQKGRGKELECSVMESAVGKPFPFPMKHCEAQAALVTHSATRTPLEIQSKGFHDLARADYAPVLKGGLEAEQKGTNETRMQPKNPSQPPPVPAKKCRERLTNGLYHPPMTPSGNNSSQEAPCLPVKKTSASNPTDFHGVPVHRTSEQEPSTPPSSLPPWLLEQPETKSVQQHVIKLGPASARKVSCSRGMDLEMVIENKLQSEGIDLTEEPYSDKHGRCGIPEALVQRYSEDLDQPEKDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGLITSVSDWLISIGLPMYSSPLTEAGFNTLSKVPSLSQTCLQEAGITEERHISKLLAAARLFKPLDPDAI

Target ORF information:

RefSeq Version	XM_025149374.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149374.1

ORF Insert Sequence:

ATGCCTAGAA TCTTACGTTG CACAGATTTA GAAATCAAAA TGTCAAGAGA ACAATCCGAT 
GATGAAACAG AGGAGTCGGT GAAGTTTAAG AGATTGCACA AGCTGGTAAA TTCTACTCGC 
AGAGTCAGGA AGAAACTCAT AAGGGTGGAA GAAATGAAAA AACCCAGTAC AGAAGGCGTA 
GAGGAGCAGT CGCTTGACAA CTCTGCTATA CTGGATGACA GATCGGCACT TTACTCTGGA 
GTTCACAAGA AGCAATTCTA CTTTGATGGT TCCTCCGAGA AGCAGACAGA GGATGACTCG 
GACTCACTTA CTACTTCTCC CTCATCTAGT AGTCTTGATA CATGGGGAGC TAGCCGGAAA 
CTGGTGAAGA CCTTCAGCAA AACTGATAGC CGTGGCCTTA TCAAGCCTCC CAAGAAACTT 
GGGACATTTT TTTCTTACCC AGAAGATGAG AAGACCCAGA AAGTTTGCCG CTCCTTGACA 
GATGGGGAGA TGAAGAAGAG CCTCGGCTCA CTGAGCCATG GGGTAAGTAC AGAAAGCATT 
TGCTTTTATG ACAGCAACAG GCACAAGCAC CATCTTCTGG TGTCCCTGGA GGAGATTCAG 
AGTGTAAGGA AGCAGATCCG ATTGAAGAAA ATGGATACTA ACTATTCCTG TTCCAGAGCT 
TTTCTTTGCC AGCGCCCTGC TAGGAAAGGA GCATCCGCCA CAACTTGCGA CCTACAATTA 
CTGCGGCACA AAGTGAAAGG GGGTGGAGGT TGTGGCTTCC CAAACAGAAG GCTACGAGGG 
CGCACTTCTG TCAGCGAGTT CAATATTACC TATGTGGTGG AGAGGAGCCT GTACAGTCAC 
CTCAACCTCT CACAGCTGGT GCGTCCCGTT ACTGACAGGA CCCTGAGCAA AGCTGACAAG 
CAGGACCTGA GGAGATGCCT GCTAGAGGAG GATGAGGAGG CCAAGAGGAA GTGGGCTGCC 
ACAGTGGATC GTTGTACTAA AAGGGTTCTC TTGAGAATCC ACCAAAAGTC TAGAACCTGC 
AGCTTTGGAG GATTTGACTT GACAAATCGT TCCCTTCATA TTGGAAACAA TTCTGACCAA 
ATGGGCAAAG AAGGTGACTT TGTTTATAAA GAAGTAATCA AATCACCCTC TGCCTCCCGT 
ATATCTCTGG GCAAAAAGGT GAAATCTGTG AAAGAAACCA TGAAGAAAAG GATGTCTAAA 
AAATACAGCA GCTCTCTGTC TGAGCAGGAG TCGAGCCCTG GAGTCATACC GGGTTCTCCG 
CAGTCGCCGC CGCCAGACAC TGACTCTCTG GACAAGCCCA AGCTGAAAGC TGGTGGCTCA 
GTTGAGAGCC TGAGGAGTTC CTTAAGTGGT CAGAGCTCAA TGAGTGGTCA GACAGTGAGT 
ACAACAGATT CCTCAACCAG CAACAGGGAG AGCGTGAAAT CAGAAGATGG GGATGATGAA 
GAACCTCCCT ACAGAGGACC CTTCTGTGGA AGAGCCAGGG TTCACACTGA TTTTACTCCA 
AGTCCTTATG ATACAGACTC TCTGAAGCTC AAGAAAGGTG ATATCATTGA CATCATCAGC 
AAACCCCCCA TGGGCACTTG GATGGGCCTA TTGAACAACA AAGTTGGCAC TTTCAAATTT 
ATCTATGTGG ATGTGTTAAA TGAAGAGGAA GAGAAGCCGA AGCGACCCAC CAGGAGACGC 
CGAAAAAGCA GACCACCCCA ACCCAAGTCA GTTGAGGATC TCTTGGATCG CATCAACTTA 
AAGGAGCACA TGCCCACTTT TCTGTTCAAT GGTTATGAAG ATCTGGATAC CTTTAAGCTT 
TTAGAAGAAG AGGATTTGGA TGAACTGAAC ATCCGAGATC CTGAGCACAG AGCTGTTCTC 
CTCACAGCCG TGGAACATCT ACAGGAGTAT GATAGTAACA GCGACCAGTC AGGATCTCAG 
GAGAAACTTC TCATTGAAGG TCAAGGCCTA GCTGGATGCT CTCCTCGGGA TTCTGGCTGC 
TATGAAAGCA GTGAAAACCT GGAGAATGGT AAAACCCGAA GGACCTGTCT TCTGCCCTCA 
AAATCATCAA GTGAGCATAG CTTTAAAGAC TTCAGCAGAC ACCAGCTATC AAATTATCCC 
ACACTCCCAC TGACCAAGTC AATAGAAACT CTACAGCAAG GAGAAAAAGA AAGCCGGTTG 
AGCTGTGCAC ATCGTGCTCT GAAGAGCTCT GTCAAGACCC CAGCTGTTAT GGGTCTGAAG 
AAGAACCGTA GAAGTTTACC AGTTGCTGTC TGTCGGAGCT ATGAAACTCT GGATGGCCCC 
CAGAGTGTGG ATGTATGGCC AAGATCTCAC TCCCTGGACG ATCTCCAGGG AGATTCCGAT 
ACTAATCTAC AAGATGGTAG CAAGAAAGAT TATTTTGCTC GGGATTCACT GGATACAGCG 
AAAAAGGCAA CTGGATCTGC CCTGCCACCT CAGTCGTGTG GGGGCAGTTG TATGGTCGAT 
AACTTGATGC CTAAGCAGGG TAAAGCAGCT GCTGGTTCTC AGAAGGGTAG AGGTAAGGAG 
TTGGAGTGCT CTGTAATGGA GAGTGCAGTT GGGAAACCTT TTCCATTCCC CATGAAACAC 
TGTGAAGCTC AGGCTGCCTT GGTGACACAT TCTGCAACAA GGACACCTCT GGAAATACAG 
AGTAAGGGCT TTCATGACCT TGCACGGGCT GACTATGCTC CAGTCCTCAA GGGAGGTCTA 
GAAGCTGAGC AAAAAGGCAC AAATGAGACA AGAATGCAAC CAAAAAATCC CTCTCAGCCG 
CCACCTGTTC CTGCCAAAAA ATGCCGAGAA CGCCTTACTA ATGGATTATA TCATCCTCCC 
ATGACCCCCA GTGGGAACAA CTCAAGTCAA GAAGCACCGT GTTTGCCAGT AAAAAAGACC 
AGTGCATCCA ATCCCACTGA TTTTCATGGA GTACCTGTCC ATAGGACATC TGAACAGGAG 
CCCAGTACCC CTCCTAGCTC ACTGCCACCC TGGCTGTTGG AGCAACCAGA GACTAAAAGC 
GTTCAGCAGC ACGTGATAAA GCTAGGGCCT GCTTCAGCAA GGAAAGTCTC TTGTAGCAGG 
GGAATGGATC TGGAAATGGT AATAGAAAAC AAGTTGCAGT CTGAAGGTAT TGATCTTACT 
GAAGAACCAT ACTCAGACAA GCATGGTCGC TGTGGCATTC CTGAGGCTTT GGTACAGAGG 
TACTCCGAAG ACTTAGACCA GCCTGAAAAG GATGTTGCTG CAAACATGGA CCAAATCCGG 
GTAAAGCAGC TGAGGAAGCA GCATCGCATG GCAATTCCAA GTGGAGGGCT CACTGAAATT 
TGCCGAAAAC CTGTATCACC AGGACTTATC ACCTCCGTAT CTGACTGGCT GATTTCCATT 
GGCCTACCCA TGTATTCCAG CCCACTCACA GAAGCAGGGT TCAACACACT GAGCAAAGTG 
CCTTCTCTGT CACAAACTTG CCTTCAGGAA GCTGGCATCA CAGAGGAAAG GCACATAAGC 
AAGCTCCTGG CAGCAGCAAG ACTCTTCAAA CCTCTCGATC CAGACGCAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH1 ( XM_025149375.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005143.1 Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OGa149136
Clone ID Related Accession (Same CDS sequence)	XM_025149375.1
Accession Version	XM_025149375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	SAM and SH3 domain-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC TGTCAAGAGA ACAATCCGAT GATGAAACAG AGGAGTCGGT GAAGTTTAAG 
AGATTGCACA AGCTGGTAAA TTCTACTCGC AGAGTCAGGA AGAAACTCAT AAGGGTGGAA 
GAAATGAAAA AACCCAGTAC AGAAGGCGTA GAGGAGCAGT CGCTTGACAA CTCTGCTATA 
CTGGATGACA GATCGGCACT TTACTCTGGA GTTCACAAGA AGCAATTCTA CTTTGATGGT 
TCCTCCGAGA AGCAGACAGA GGATGACTCG GACTCACTTA CTACTTCTCC CTCATCTAGT 
AGTCTTGATA CATGGGGAGC TAGCCGGAAA CTGGTGAAGA CCTTCAGCAA AACTGATAGC 
CGTGGCCTTA TCAAGCCTCC CAAGAAACTT GGGACATTTT TTTCTTACCC AGAAGATGAG 
AAGACCCAGA AAGTTTGCCG CTCCTTGACA GATGGGGAGA TGAAGAAGAG CCTCGGCTCA 
CTGAGCCATG GGGTAAGTAC AGAAAGCATT TGCTTTTATG ACAGCAACAG GCACAAGCAC 
CATCTTCTGG TGTCCCTGGA GGAGATTCAG AGTGTAAGGA AGCAGATCCG ATTGAAGAAA 
ATGGATACTA ACTATTCCTG TTCCAGAGCT TTTCTTTGCC AGCGCCCTGC TAGGAAAGGA 
GCATCCGCCA CAACTTGCGA CCTACAATTA CTGCGGCACA AAGTGAAAGG GGGTGGAGGT 
TGTGGCTTCC CAAACAGAAG GCTACGAGGG CGCACTTCTG TCAGCGAGTT CAATATTACC 
TATGTGGTGG AGAGGAGCCT GTACAGTCAC CTCAACCTCT CACAGCTGGT GCGTCCCGTT 
ACTGACAGGA CCCTGAGCAA AGCTGACAAG CAGGACCTGA GGAGATGCCT GCTAGAGGAG 
GATGAGGAGG CCAAGAGGAA GTGGGCTGCC ACAGTGGATC GTTGTACTAA AAGGGTTCTC 
TTGAGAATCC ACCAAAAGTC TAGAACCTGC AGCTTTGGAG GATTTGACTT GACAAATCGT 
TCCCTTCATA TTGGAAACAA TTCTGACCAA ATGGGCAAAG AAGGTGACTT TGTTTATAAA 
GAAGTAATCA AATCACCCTC TGCCTCCCGT ATATCTCTGG GCAAAAAGGT GAAATCTGTG 
AAAGAAACCA TGAAGAAAAG GATGTCTAAA AAATACAGCA GCTCTCTGTC TGAGCAGGAG 
TCGAGCCCTG GAGTCATACC GGGTTCTCCG CAGTCGCCGC CGCCAGACAC TGACTCTCTG 
GACAAGCCCA AGCTGAAAGC TGGTGGCTCA GTTGAGAGCC TGAGGAGTTC CTTAAGTGGT 
CAGAGCTCAA TGAGTGGTCA GACAGTGAGT ACAACAGATT CCTCAACCAG CAACAGGGAG 
AGCGTGAAAT CAGAAGATGG GGATGATGAA GAACCTCCCT ACAGAGGACC CTTCTGTGGA 
AGAGCCAGGG TTCACACTGA TTTTACTCCA AGTCCTTATG ATACAGACTC TCTGAAGCTC 
AAGAAAGGTG ATATCATTGA CATCATCAGC AAACCCCCCA TGGGCACTTG GATGGGCCTA 
TTGAACAACA AAGTTGGCAC TTTCAAATTT ATCTATGTGG ATGTGTTAAA TGAAGAGGAA 
GAGAAGCCGA AGCGACCCAC CAGGAGACGC CGAAAAAGCA GACCACCCCA ACCCAAGTCA 
GTTGAGGATC TCTTGGATCG CATCAACTTA AAGGAGCACA TGCCCACTTT TCTGTTCAAT 
GGTTATGAAG ATCTGGATAC CTTTAAGCTT TTAGAAGAAG AGGATTTGGA TGAACTGAAC 
ATCCGAGATC CTGAGCACAG AGCTGTTCTC CTCACAGCCG TGGAACATCT ACAGGAGTAT 
GATAGTAACA GCGACCAGTC AGGATCTCAG GAGAAACTTC TCATTGAAGG TCAAGGCCTA 
GCTGGATGCT CTCCTCGGGA TTCTGGCTGC TATGAAAGCA GTGAAAACCT GGAGAATGGT 
AAAACCCGAA GGACCTGTCT TCTGCCCTCA AAATCATCAA GTGAGCATAG CTTTAAAGAC 
TTCAGCAGAC ACCAGCTATC AAATTATCCC ACACTCCCAC TGACCAAGTC AATAGAAACT 
CTACAGCAAG GAGAAAAAGA AAGCCGGTTG AGCTGTGCAC ATCGTGCTCT GAAGAGCTCT 
GTCAAGACCC CAGCTGTTAT GGGTCTGAAG AAGAACCGTA GAAGTTTACC AGTTGCTGTC 
TGTCGGAGCT ATGAAACTCT GGATGGCCCC CAGAGTGTGG ATGTATGGCC AAGATCTCAC 
TCCCTGGACG ATCTCCAGGG AGATTCCGAT ACTAATCTAC AAGATGGTAG CAAGAAAGAT 
TATTTTGCTC GGGATTCACT GGATACAGCG AAAAAGGCAA CTGGATCTGC CCTGCCACCT 
CAGTCGTGTG GGGGCAGTTG TATGGTCGAT AACTTGATGC CTAAGCAGGG TAAAGCAGCT 
GCTGGTTCTC AGAAGGGTAG AGGTAAGGAG TTGGAGTGCT CTGTAATGGA GAGTGCAGTT 
GGGAAACCTT TTCCATTCCC CATGAAACAC TGTGAAGCTC AGGCTGCCTT GGTGACACAT 
TCTGCAACAA GGACACCTCT GGAAATACAG AGTAAGGGCT TTCATGACCT TGCACGGGCT 
GACTATGCTC CAGTCCTCAA GGGAGGTCTA GAAGCTGAGC AAAAAGGCAC AAATGAGACA 
AGAATGCAAC CAAAAAATCC CTCTCAGCCG CCACCTGTTC CTGCCAAAAA ATGCCGAGAA 
CGCCTTACTA ATGGATTATA TCATCCTCCC ATGACCCCCA GTGGGAACAA CTCAAGTCAA 
GAAGCACCGT GTTTGCCAGT AAAAAAGACC AGTGCATCCA ATCCCACTGA TTTTCATGGA 
GTACCTGTCC ATAGGACATC TGAACAGGAG CCCAGTACCC CTCCTAGCTC ACTGCCACCC 
TGGCTGTTGG AGCAACCAGA GACTAAAAGC GTTCAGCAGC ACGTGATAAA GCTAGGGCCT 
GCTTCAGCAA GGAAAGTCTC TTGTAGCAGG GGAATGGATC TGGAAATGGT AATAGAAAAC 
AAGTTGCAGT CTGAAGGTAT TGATCTTACT GAAGAACCAT ACTCAGACAA GCATGGTCGC 
TGTGGCATTC CTGAGGCTTT GGTACAGAGG TACTCCGAAG ACTTAGACCA GCCTGAAAAG 
GATGTTGCTG CAAACATGGA CCAAATCCGG GTAAAGCAGC TGAGGAAGCA GCATCGCATG 
GCAATTCCAA GTGGAGGGCT CACTGAAATT TGCCGAAAAC CTGTATCACC AGGACTTATC 
ACCTCCGTAT CTGACTGGCT GATTTCCATT GGCCTACCCA TGTATTCCAG CCCACTCACA 
GAAGCAGGGT TCAACACACT GAGCAAAGTG CCTTCTCTGT CACAAACTTG CCTTCAGGAA 
GCTGGCATCA CAGAGGAAAG GCACATAAGC AAGCTCCTGG CAGCAGCAAG ACTCTTCAAA 
CCTCTCGATC CAGACGCAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005143.1
CDS	206..3709
Translation	MPKLSREQSDDETEESVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGVEEQSLDNSAILDDRSALYSGVHKKQFYFDGSSEKQTEDDSDSLTTSPSSSSLDTWGASRKLVKTFSKTDSRGLIKPPKKLGTFFSYPEDEKTQKVCRSLTDGEMKKSLGSLSHGVSTESICFYDSNRHKHHLLVSLEEIQSVRKQIRLKKMDTNYSCSRAFLCQRPARKGASATTCDLQLLRHKVKGGGGCGFPNRRLRGRTSVSEFNITYVVERSLYSHLNLSQLVRPVTDRTLSKADKQDLRRCLLEEDEEAKRKWAATVDRCTKRVLLRIHQKSRTCSFGGFDLTNRSLHIGNNSDQMGKEGDFVYKEVIKSPSASRISLGKKVKSVKETMKKRMSKKYSSSLSEQESSPGVIPGSPQSPPPDTDSLDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRRKSRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVEHLQEYDSNSDQSGSQEKLLIEGQGLAGCSPRDSGCYESSENLENGKTRRTCLLPSKSSSEHSFKDFSRHQLSNYPTLPLTKSIETLQQGEKESRLSCAHRALKSSVKTPAVMGLKKNRRSLPVAVCRSYETLDGPQSVDVWPRSHSLDDLQGDSDTNLQDGSKKDYFARDSLDTAKKATGSALPPQSCGGSCMVDNLMPKQGKAAAGSQKGRGKELECSVMESAVGKPFPFPMKHCEAQAALVTHSATRTPLEIQSKGFHDLARADYAPVLKGGLEAEQKGTNETRMQPKNPSQPPPVPAKKCRERLTNGLYHPPMTPSGNNSSQEAPCLPVKKTSASNPTDFHGVPVHRTSEQEPSTPPSSLPPWLLEQPETKSVQQHVIKLGPASARKVSCSRGMDLEMVIENKLQSEGIDLTEEPYSDKHGRCGIPEALVQRYSEDLDQPEKDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGLITSVSDWLISIGLPMYSSPLTEAGFNTLSKVPSLSQTCLQEAGITEERHISKLLAAARLFKPLDPDAI

Target ORF information:

RefSeq Version	XM_025149375.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149375.1

ORF Insert Sequence:

ATGCCGAAGC TGTCAAGAGA ACAATCCGAT GATGAAACAG AGGAGTCGGT GAAGTTTAAG 
AGATTGCACA AGCTGGTAAA TTCTACTCGC AGAGTCAGGA AGAAACTCAT AAGGGTGGAA 
GAAATGAAAA AACCCAGTAC AGAAGGCGTA GAGGAGCAGT CGCTTGACAA CTCTGCTATA 
CTGGATGACA GATCGGCACT TTACTCTGGA GTTCACAAGA AGCAATTCTA CTTTGATGGT 
TCCTCCGAGA AGCAGACAGA GGATGACTCG GACTCACTTA CTACTTCTCC CTCATCTAGT 
AGTCTTGATA CATGGGGAGC TAGCCGGAAA CTGGTGAAGA CCTTCAGCAA AACTGATAGC 
CGTGGCCTTA TCAAGCCTCC CAAGAAACTT GGGACATTTT TTTCTTACCC AGAAGATGAG 
AAGACCCAGA AAGTTTGCCG CTCCTTGACA GATGGGGAGA TGAAGAAGAG CCTCGGCTCA 
CTGAGCCATG GGGTAAGTAC AGAAAGCATT TGCTTTTATG ACAGCAACAG GCACAAGCAC 
CATCTTCTGG TGTCCCTGGA GGAGATTCAG AGTGTAAGGA AGCAGATCCG ATTGAAGAAA 
ATGGATACTA ACTATTCCTG TTCCAGAGCT TTTCTTTGCC AGCGCCCTGC TAGGAAAGGA 
GCATCCGCCA CAACTTGCGA CCTACAATTA CTGCGGCACA AAGTGAAAGG GGGTGGAGGT 
TGTGGCTTCC CAAACAGAAG GCTACGAGGG CGCACTTCTG TCAGCGAGTT CAATATTACC 
TATGTGGTGG AGAGGAGCCT GTACAGTCAC CTCAACCTCT CACAGCTGGT GCGTCCCGTT 
ACTGACAGGA CCCTGAGCAA AGCTGACAAG CAGGACCTGA GGAGATGCCT GCTAGAGGAG 
GATGAGGAGG CCAAGAGGAA GTGGGCTGCC ACAGTGGATC GTTGTACTAA AAGGGTTCTC 
TTGAGAATCC ACCAAAAGTC TAGAACCTGC AGCTTTGGAG GATTTGACTT GACAAATCGT 
TCCCTTCATA TTGGAAACAA TTCTGACCAA ATGGGCAAAG AAGGTGACTT TGTTTATAAA 
GAAGTAATCA AATCACCCTC TGCCTCCCGT ATATCTCTGG GCAAAAAGGT GAAATCTGTG 
AAAGAAACCA TGAAGAAAAG GATGTCTAAA AAATACAGCA GCTCTCTGTC TGAGCAGGAG 
TCGAGCCCTG GAGTCATACC GGGTTCTCCG CAGTCGCCGC CGCCAGACAC TGACTCTCTG 
GACAAGCCCA AGCTGAAAGC TGGTGGCTCA GTTGAGAGCC TGAGGAGTTC CTTAAGTGGT 
CAGAGCTCAA TGAGTGGTCA GACAGTGAGT ACAACAGATT CCTCAACCAG CAACAGGGAG 
AGCGTGAAAT CAGAAGATGG GGATGATGAA GAACCTCCCT ACAGAGGACC CTTCTGTGGA 
AGAGCCAGGG TTCACACTGA TTTTACTCCA AGTCCTTATG ATACAGACTC TCTGAAGCTC 
AAGAAAGGTG ATATCATTGA CATCATCAGC AAACCCCCCA TGGGCACTTG GATGGGCCTA 
TTGAACAACA AAGTTGGCAC TTTCAAATTT ATCTATGTGG ATGTGTTAAA TGAAGAGGAA 
GAGAAGCCGA AGCGACCCAC CAGGAGACGC CGAAAAAGCA GACCACCCCA ACCCAAGTCA 
GTTGAGGATC TCTTGGATCG CATCAACTTA AAGGAGCACA TGCCCACTTT TCTGTTCAAT 
GGTTATGAAG ATCTGGATAC CTTTAAGCTT TTAGAAGAAG AGGATTTGGA TGAACTGAAC 
ATCCGAGATC CTGAGCACAG AGCTGTTCTC CTCACAGCCG TGGAACATCT ACAGGAGTAT 
GATAGTAACA GCGACCAGTC AGGATCTCAG GAGAAACTTC TCATTGAAGG TCAAGGCCTA 
GCTGGATGCT CTCCTCGGGA TTCTGGCTGC TATGAAAGCA GTGAAAACCT GGAGAATGGT 
AAAACCCGAA GGACCTGTCT TCTGCCCTCA AAATCATCAA GTGAGCATAG CTTTAAAGAC 
TTCAGCAGAC ACCAGCTATC AAATTATCCC ACACTCCCAC TGACCAAGTC AATAGAAACT 
CTACAGCAAG GAGAAAAAGA AAGCCGGTTG AGCTGTGCAC ATCGTGCTCT GAAGAGCTCT 
GTCAAGACCC CAGCTGTTAT GGGTCTGAAG AAGAACCGTA GAAGTTTACC AGTTGCTGTC 
TGTCGGAGCT ATGAAACTCT GGATGGCCCC CAGAGTGTGG ATGTATGGCC AAGATCTCAC 
TCCCTGGACG ATCTCCAGGG AGATTCCGAT ACTAATCTAC AAGATGGTAG CAAGAAAGAT 
TATTTTGCTC GGGATTCACT GGATACAGCG AAAAAGGCAA CTGGATCTGC CCTGCCACCT 
CAGTCGTGTG GGGGCAGTTG TATGGTCGAT AACTTGATGC CTAAGCAGGG TAAAGCAGCT 
GCTGGTTCTC AGAAGGGTAG AGGTAAGGAG TTGGAGTGCT CTGTAATGGA GAGTGCAGTT 
GGGAAACCTT TTCCATTCCC CATGAAACAC TGTGAAGCTC AGGCTGCCTT GGTGACACAT 
TCTGCAACAA GGACACCTCT GGAAATACAG AGTAAGGGCT TTCATGACCT TGCACGGGCT 
GACTATGCTC CAGTCCTCAA GGGAGGTCTA GAAGCTGAGC AAAAAGGCAC AAATGAGACA 
AGAATGCAAC CAAAAAATCC CTCTCAGCCG CCACCTGTTC CTGCCAAAAA ATGCCGAGAA 
CGCCTTACTA ATGGATTATA TCATCCTCCC ATGACCCCCA GTGGGAACAA CTCAAGTCAA 
GAAGCACCGT GTTTGCCAGT AAAAAAGACC AGTGCATCCA ATCCCACTGA TTTTCATGGA 
GTACCTGTCC ATAGGACATC TGAACAGGAG CCCAGTACCC CTCCTAGCTC ACTGCCACCC 
TGGCTGTTGG AGCAACCAGA GACTAAAAGC GTTCAGCAGC ACGTGATAAA GCTAGGGCCT 
GCTTCAGCAA GGAAAGTCTC TTGTAGCAGG GGAATGGATC TGGAAATGGT AATAGAAAAC 
AAGTTGCAGT CTGAAGGTAT TGATCTTACT GAAGAACCAT ACTCAGACAA GCATGGTCGC 
TGTGGCATTC CTGAGGCTTT GGTACAGAGG TACTCCGAAG ACTTAGACCA GCCTGAAAAG 
GATGTTGCTG CAAACATGGA CCAAATCCGG GTAAAGCAGC TGAGGAAGCA GCATCGCATG 
GCAATTCCAA GTGGAGGGCT CACTGAAATT TGCCGAAAAC CTGTATCACC AGGACTTATC 
ACCTCCGTAT CTGACTGGCT GATTTCCATT GGCCTACCCA TGTATTCCAG CCCACTCACA 
GAAGCAGGGT TCAACACACT GAGCAAAGTG CCTTCTCTGT CACAAACTTG CCTTCAGGAA 
GCTGGCATCA CAGAGGAAAG GCACATAAGC AAGCTCCTGG CAGCAGCAAG ACTCTTCAAA 
CCTCTCGATC CAGACGCAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH1 ( XM_025149376.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005144.1 Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OGa149137
Clone ID Related Accession (Same CDS sequence)	XM_025149376.1
Accession Version	XM_025149376.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	SAM and SH3 domain-containing protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAC CCAGTACAGA AGGCGTAGAG GAGCAGTCGC TTGACAACTC TGCTATACTG 
GATGACAGAT CGGCACTTTA CTCTGGAGTT CACAAGAAGC AATTCTACTT TGATGGTTCC 
TCCGAGAAGC AGACAGAGGA TGACTCGGAC TCACTTACTA CTTCTCCCTC ATCTAGTAGT 
CTTGATACAT GGGGAGCTAG CCGGAAACTG GTGAAGACCT TCAGCAAAAC TGATAGCCGT 
GGCCTTATCA AGCCTCCCAA GAAACTTGGG ACATTTTTTT CTTACCCAGA AGATGAGAAG 
ACCCAGAAAG TTTGCCGCTC CTTGACAGAT GGGGAGATGA AGAAGAGCCT CGGCTCACTG 
AGCCATGGGG TAAGTACAGA AAGCATTTGC TTTTATGACA GCAACAGGCA CAAGCACCAT 
CTTCTGGTGT CCCTGGAGGA GATTCAGAGT GTAAGGAAGC AGATCCGATT GAAGAAAATG 
GATACTAACT ATTCCTGTTC CAGAGCTTTT CTTTGCCAGC GCCCTGCTAG GAAAGGAGCA 
TCCGCCACAA CTTGCGACCT ACAATTACTG CGGCACAAAG TGAAAGGGGG TGGAGGTTGT 
GGCTTCCCAA ACAGAAGGCT ACGAGGGCGC ACTTCTGTCA GCGAGTTCAA TATTACCTAT 
GTGGTGGAGA GGAGCCTGTA CAGTCACCTC AACCTCTCAC AGCTGGTGCG TCCCGTTACT 
GACAGGACCC TGAGCAAAGC TGACAAGCAG GACCTGAGGA GATGCCTGCT AGAGGAGGAT 
GAGGAGGCCA AGAGGAAGTG GGCTGCCACA GTGGATCGTT GTACTAAAAG GGTTCTCTTG 
AGAATCCACC AAAAGTCTAG AACCTGCAGC TTTGGAGGAT TTGACTTGAC AAATCGTTCC 
CTTCATATTG GAAACAATTC TGACCAAATG GGCAAAGAAG GTGACTTTGT TTATAAAGAA 
GTAATCAAAT CACCCTCTGC CTCCCGTATA TCTCTGGGCA AAAAGGTGAA ATCTGTGAAA 
GAAACCATGA AGAAAAGGAT GTCTAAAAAA TACAGCAGCT CTCTGTCTGA GCAGGAGTCG 
AGCCCTGGAG TCATACCGGG TTCTCCGCAG TCGCCGCCGC CAGACACTGA CTCTCTGGAC 
AAGCCCAAGC TGAAAGCTGG TGGCTCAGTT GAGAGCCTGA GGAGTTCCTT AAGTGGTCAG 
AGCTCAATGA GTGGTCAGAC AGTGAGTACA ACAGATTCCT CAACCAGCAA CAGGGAGAGC 
GTGAAATCAG AAGATGGGGA TGATGAAGAA CCTCCCTACA GAGGACCCTT CTGTGGAAGA 
GCCAGGGTTC ACACTGATTT TACTCCAAGT CCTTATGATA CAGACTCTCT GAAGCTCAAG 
AAAGGTGATA TCATTGACAT CATCAGCAAA CCCCCCATGG GCACTTGGAT GGGCCTATTG 
AACAACAAAG TTGGCACTTT CAAATTTATC TATGTGGATG TGTTAAATGA AGAGGAAGAG 
AAGCCGAAGC GACCCACCAG GAGACGCCGA AAAAGCAGAC CACCCCAACC CAAGTCAGTT 
GAGGATCTCT TGGATCGCAT CAACTTAAAG GAGCACATGC CCACTTTTCT GTTCAATGGT 
TATGAAGATC TGGATACCTT TAAGCTTTTA GAAGAAGAGG ATTTGGATGA ACTGAACATC 
CGAGATCCTG AGCACAGAGC TGTTCTCCTC ACAGCCGTGG AACATCTACA GGAGTATGAT 
AGTAACAGCG ACCAGTCAGG ATCTCAGGAG AAACTTCTCA TTGAAGGTCA AGGCCTAGCT 
GGATGCTCTC CTCGGGATTC TGGCTGCTAT GAAAGCAGTG AAAACCTGGA GAATGGTAAA 
ACCCGAAGGA CCTGTCTTCT GCCCTCAAAA TCATCAAGTG AGCATAGCTT TAAAGACTTC 
AGCAGACACC AGCTATCAAA TTATCCCACA CTCCCACTGA CCAAGTCAAT AGAAACTCTA 
CAGCAAGGAG AAAAAGAAAG CCGGTTGAGC TGTGCACATC GTGCTCTGAA GAGCTCTGTC 
AAGACCCCAG CTGTTATGGG TCTGAAGAAG AACCGTAGAA GTTTACCAGT TGCTGTCTGT 
CGGAGCTATG AAACTCTGGA TGGCCCCCAG AGTGTGGATG TATGGCCAAG ATCTCACTCC 
CTGGACGATC TCCAGGGAGA TTCCGATACT AATCTACAAG ATGGTAGCAA GAAAGATTAT 
TTTGCTCGGG ATTCACTGGA TACAGCGAAA AAGGCAACTG GATCTGCCCT GCCACCTCAG 
TCGTGTGGGG GCAGTTGTAT GGTCGATAAC TTGATGCCTA AGCAGGGTAA AGCAGCTGCT 
GGTTCTCAGA AGGGTAGAGG TAAGGAGTTG GAGTGCTCTG TAATGGAGAG TGCAGTTGGG 
AAACCTTTTC CATTCCCCAT GAAACACTGT GAAGCTCAGG CTGCCTTGGT GACACATTCT 
GCAACAAGGA CACCTCTGGA AATACAGAGT AAGGGCTTTC ATGACCTTGC ACGGGCTGAC 
TATGCTCCAG TCCTCAAGGG AGGTCTAGAA GCTGAGCAAA AAGGCACAAA TGAGACAAGA 
ATGCAACCAA AAAATCCCTC TCAGCCGCCA CCTGTTCCTG CCAAAAAATG CCGAGAACGC 
CTTACTAATG GATTATATCA TCCTCCCATG ACCCCCAGTG GGAACAACTC AAGTCAAGAA 
GCACCGTGTT TGCCAGTAAA AAAGACCAGT GCATCCAATC CCACTGATTT TCATGGAGTA 
CCTGTCCATA GGACATCTGA ACAGGAGCCC AGTACCCCTC CTAGCTCACT GCCACCCTGG 
CTGTTGGAGC AACCAGAGAC TAAAAGCGTT CAGCAGCACG TGATAAAGCT AGGGCCTGCT 
TCAGCAAGGA AAGTCTCTTG TAGCAGGGGA ATGGATCTGG AAATGGTAAT AGAAAACAAG 
TTGCAGTCTG AAGGTATTGA TCTTACTGAA GAACCATACT CAGACAAGCA TGGTCGCTGT 
GGCATTCCTG AGGCTTTGGT ACAGAGGTAC TCCGAAGACT TAGACCAGCC TGAAAAGGAT 
GTTGCTGCAA ACATGGACCA AATCCGGGTA AAGCAGCTGA GGAAGCAGCA TCGCATGGCA 
ATTCCAAGTG GAGGGCTCAC TGAAATTTGC CGAAAACCTG TATCACCAGG ACTTATCACC 
TCCGTATCTG ACTGGCTGAT TTCCATTGGC CTACCCATGT ATTCCAGCCC ACTCACAGAA 
GCAGGGTTCA ACACACTGAG CAAAGTGCCT TCTCTGTCAC AAACTTGCCT TCAGGAAGCT 
GGCATCACAG AGGAAAGGCA CATAAGCAAG CTCCTGGCAG CAGCAAGACT CTTCAAACCT 
CTCGATCCAG ACGCAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005144.1
CDS	451..3831
Translation	MKKPSTEGVEEQSLDNSAILDDRSALYSGVHKKQFYFDGSSEKQTEDDSDSLTTSPSSSSLDTWGASRKLVKTFSKTDSRGLIKPPKKLGTFFSYPEDEKTQKVCRSLTDGEMKKSLGSLSHGVSTESICFYDSNRHKHHLLVSLEEIQSVRKQIRLKKMDTNYSCSRAFLCQRPARKGASATTCDLQLLRHKVKGGGGCGFPNRRLRGRTSVSEFNITYVVERSLYSHLNLSQLVRPVTDRTLSKADKQDLRRCLLEEDEEAKRKWAATVDRCTKRVLLRIHQKSRTCSFGGFDLTNRSLHIGNNSDQMGKEGDFVYKEVIKSPSASRISLGKKVKSVKETMKKRMSKKYSSSLSEQESSPGVIPGSPQSPPPDTDSLDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRRKSRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVEHLQEYDSNSDQSGSQEKLLIEGQGLAGCSPRDSGCYESSENLENGKTRRTCLLPSKSSSEHSFKDFSRHQLSNYPTLPLTKSIETLQQGEKESRLSCAHRALKSSVKTPAVMGLKKNRRSLPVAVCRSYETLDGPQSVDVWPRSHSLDDLQGDSDTNLQDGSKKDYFARDSLDTAKKATGSALPPQSCGGSCMVDNLMPKQGKAAAGSQKGRGKELECSVMESAVGKPFPFPMKHCEAQAALVTHSATRTPLEIQSKGFHDLARADYAPVLKGGLEAEQKGTNETRMQPKNPSQPPPVPAKKCRERLTNGLYHPPMTPSGNNSSQEAPCLPVKKTSASNPTDFHGVPVHRTSEQEPSTPPSSLPPWLLEQPETKSVQQHVIKLGPASARKVSCSRGMDLEMVIENKLQSEGIDLTEEPYSDKHGRCGIPEALVQRYSEDLDQPEKDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGLITSVSDWLISIGLPMYSSPLTEAGFNTLSKVPSLSQTCLQEAGITEERHISKLLAAARLFKPLDPDAI

Target ORF information:

RefSeq Version	XM_025149376.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149376.1

ORF Insert Sequence:

ATGAAAAAAC CCAGTACAGA AGGCGTAGAG GAGCAGTCGC TTGACAACTC TGCTATACTG 
GATGACAGAT CGGCACTTTA CTCTGGAGTT CACAAGAAGC AATTCTACTT TGATGGTTCC 
TCCGAGAAGC AGACAGAGGA TGACTCGGAC TCACTTACTA CTTCTCCCTC ATCTAGTAGT 
CTTGATACAT GGGGAGCTAG CCGGAAACTG GTGAAGACCT TCAGCAAAAC TGATAGCCGT 
GGCCTTATCA AGCCTCCCAA GAAACTTGGG ACATTTTTTT CTTACCCAGA AGATGAGAAG 
ACCCAGAAAG TTTGCCGCTC CTTGACAGAT GGGGAGATGA AGAAGAGCCT CGGCTCACTG 
AGCCATGGGG TAAGTACAGA AAGCATTTGC TTTTATGACA GCAACAGGCA CAAGCACCAT 
CTTCTGGTGT CCCTGGAGGA GATTCAGAGT GTAAGGAAGC AGATCCGATT GAAGAAAATG 
GATACTAACT ATTCCTGTTC CAGAGCTTTT CTTTGCCAGC GCCCTGCTAG GAAAGGAGCA 
TCCGCCACAA CTTGCGACCT ACAATTACTG CGGCACAAAG TGAAAGGGGG TGGAGGTTGT 
GGCTTCCCAA ACAGAAGGCT ACGAGGGCGC ACTTCTGTCA GCGAGTTCAA TATTACCTAT 
GTGGTGGAGA GGAGCCTGTA CAGTCACCTC AACCTCTCAC AGCTGGTGCG TCCCGTTACT 
GACAGGACCC TGAGCAAAGC TGACAAGCAG GACCTGAGGA GATGCCTGCT AGAGGAGGAT 
GAGGAGGCCA AGAGGAAGTG GGCTGCCACA GTGGATCGTT GTACTAAAAG GGTTCTCTTG 
AGAATCCACC AAAAGTCTAG AACCTGCAGC TTTGGAGGAT TTGACTTGAC AAATCGTTCC 
CTTCATATTG GAAACAATTC TGACCAAATG GGCAAAGAAG GTGACTTTGT TTATAAAGAA 
GTAATCAAAT CACCCTCTGC CTCCCGTATA TCTCTGGGCA AAAAGGTGAA ATCTGTGAAA 
GAAACCATGA AGAAAAGGAT GTCTAAAAAA TACAGCAGCT CTCTGTCTGA GCAGGAGTCG 
AGCCCTGGAG TCATACCGGG TTCTCCGCAG TCGCCGCCGC CAGACACTGA CTCTCTGGAC 
AAGCCCAAGC TGAAAGCTGG TGGCTCAGTT GAGAGCCTGA GGAGTTCCTT AAGTGGTCAG 
AGCTCAATGA GTGGTCAGAC AGTGAGTACA ACAGATTCCT CAACCAGCAA CAGGGAGAGC 
GTGAAATCAG AAGATGGGGA TGATGAAGAA CCTCCCTACA GAGGACCCTT CTGTGGAAGA 
GCCAGGGTTC ACACTGATTT TACTCCAAGT CCTTATGATA CAGACTCTCT GAAGCTCAAG 
AAAGGTGATA TCATTGACAT CATCAGCAAA CCCCCCATGG GCACTTGGAT GGGCCTATTG 
AACAACAAAG TTGGCACTTT CAAATTTATC TATGTGGATG TGTTAAATGA AGAGGAAGAG 
AAGCCGAAGC GACCCACCAG GAGACGCCGA AAAAGCAGAC CACCCCAACC CAAGTCAGTT 
GAGGATCTCT TGGATCGCAT CAACTTAAAG GAGCACATGC CCACTTTTCT GTTCAATGGT 
TATGAAGATC TGGATACCTT TAAGCTTTTA GAAGAAGAGG ATTTGGATGA ACTGAACATC 
CGAGATCCTG AGCACAGAGC TGTTCTCCTC ACAGCCGTGG AACATCTACA GGAGTATGAT 
AGTAACAGCG ACCAGTCAGG ATCTCAGGAG AAACTTCTCA TTGAAGGTCA AGGCCTAGCT 
GGATGCTCTC CTCGGGATTC TGGCTGCTAT GAAAGCAGTG AAAACCTGGA GAATGGTAAA 
ACCCGAAGGA CCTGTCTTCT GCCCTCAAAA TCATCAAGTG AGCATAGCTT TAAAGACTTC 
AGCAGACACC AGCTATCAAA TTATCCCACA CTCCCACTGA CCAAGTCAAT AGAAACTCTA 
CAGCAAGGAG AAAAAGAAAG CCGGTTGAGC TGTGCACATC GTGCTCTGAA GAGCTCTGTC 
AAGACCCCAG CTGTTATGGG TCTGAAGAAG AACCGTAGAA GTTTACCAGT TGCTGTCTGT 
CGGAGCTATG AAACTCTGGA TGGCCCCCAG AGTGTGGATG TATGGCCAAG ATCTCACTCC 
CTGGACGATC TCCAGGGAGA TTCCGATACT AATCTACAAG ATGGTAGCAA GAAAGATTAT 
TTTGCTCGGG ATTCACTGGA TACAGCGAAA AAGGCAACTG GATCTGCCCT GCCACCTCAG 
TCGTGTGGGG GCAGTTGTAT GGTCGATAAC TTGATGCCTA AGCAGGGTAA AGCAGCTGCT 
GGTTCTCAGA AGGGTAGAGG TAAGGAGTTG GAGTGCTCTG TAATGGAGAG TGCAGTTGGG 
AAACCTTTTC CATTCCCCAT GAAACACTGT GAAGCTCAGG CTGCCTTGGT GACACATTCT 
GCAACAAGGA CACCTCTGGA AATACAGAGT AAGGGCTTTC ATGACCTTGC ACGGGCTGAC 
TATGCTCCAG TCCTCAAGGG AGGTCTAGAA GCTGAGCAAA AAGGCACAAA TGAGACAAGA 
ATGCAACCAA AAAATCCCTC TCAGCCGCCA CCTGTTCCTG CCAAAAAATG CCGAGAACGC 
CTTACTAATG GATTATATCA TCCTCCCATG ACCCCCAGTG GGAACAACTC AAGTCAAGAA 
GCACCGTGTT TGCCAGTAAA AAAGACCAGT GCATCCAATC CCACTGATTT TCATGGAGTA 
CCTGTCCATA GGACATCTGA ACAGGAGCCC AGTACCCCTC CTAGCTCACT GCCACCCTGG 
CTGTTGGAGC AACCAGAGAC TAAAAGCGTT CAGCAGCACG TGATAAAGCT AGGGCCTGCT 
TCAGCAAGGA AAGTCTCTTG TAGCAGGGGA ATGGATCTGG AAATGGTAAT AGAAAACAAG 
TTGCAGTCTG AAGGTATTGA TCTTACTGAA GAACCATACT CAGACAAGCA TGGTCGCTGT 
GGCATTCCTG AGGCTTTGGT ACAGAGGTAC TCCGAAGACT TAGACCAGCC TGAAAAGGAT 
GTTGCTGCAA ACATGGACCA AATCCGGGTA AAGCAGCTGA GGAAGCAGCA TCGCATGGCA 
ATTCCAAGTG GAGGGCTCAC TGAAATTTGC CGAAAACCTG TATCACCAGG ACTTATCACC 
TCCGTATCTG ACTGGCTGAT TTCCATTGGC CTACCCATGT ATTCCAGCCC ACTCACAGAA 
GCAGGGTTCA ACACACTGAG CAAAGTGCCT TCTCTGTCAC AAACTTGCCT TCAGGAAGCT 
GGCATCACAG AGGAAAGGCA CATAAGCAAG CTCCTGGCAG CAGCAAGACT CTTCAAACCT 
CTCGATCCAG ACGCAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH1 ( XM_025149377.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005145.1 Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OGa149138
Clone ID Related Accession (Same CDS sequence)	XM_025149377.1
Accession Version	XM_025149377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	SAM and SH3 domain-containing protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGC TGTCAAGAGA ACAATCCGAT GATGAAACAG AGGAGTCGGT GAAGTTTAAG 
AGATTGCACA AGCTGGTAAA TTCTACTCGC AGAGTCAGGA AGAAACTCAT AAGGGTGGAA 
GAAATGAAAA AACCCAGTAC AGAAGGCGTA GAGGAGCAGT CGCTTGACAA CTCTGCTATA 
CTGGATGACA GATCGGCACT TTACTCTGGA GTTCACAAGA AGCAATTCTA CTTTGATGGT 
TCCTCCGAGA AGCAGACAGA GGATGACTCG GACTCACTTA CTACTTCTCC CTCATCTAGT 
AGTCTTGATA CATGGGGAGC TAGCCGGAAA CTGGTGAAGA CCTTCAGCAA AACTGATAGC 
CGTGGCCTTA TCAAGCCTCC CAAGAAACTT GGGACATTTT TTTCTTACCC AGAAGATGAG 
AAGACCCAGA AAGTTTGCCG CTCCTTGACA GATGGGGAGA TGAAGAAGAG CCTCGGCTCA 
CTGAGCCATG GGAGAACCTG CAGCTTTGGA GGATTTGACT TGACAAATCG TTCCCTTCAT 
ATTGGAAACA ATTCTGACCA AATGGGCAAA GAAGGTGACT TTGTTTATAA AGAAGTAATC 
AAATCACCCT CTGCCTCCCG TATATCTCTG GGCAAAAAGG TGAAATCTGT GAAAGAAACC 
ATGAAGAAAA GGATGTCTAA AAAATACAGC AGCTCTCTGT CTGAGCAGGA GTCGAGCCCT 
GGAGTCATAC CGGGTTCTCC GCAGTCGCCG CCGCCAGACA CTGACTCTCT GGACAAGCCC 
AAGCTGAAAG CTGGTGGCTC AGTTGAGAGC CTGAGGAGTT CCTTAAGTGG TCAGAGCTCA 
ATGAGTGGTC AGACAGTGAG TACAACAGAT TCCTCAACCA GCAACAGGGA GAGCGTGAAA 
TCAGAAGATG GGGATGATGA AGAACCTCCC TACAGAGGAC CCTTCTGTGG AAGAGCCAGG 
GTTCACACTG ATTTTACTCC AAGTCCTTAT GATACAGACT CTCTGAAGCT CAAGAAAGGT 
GATATCATTG ACATCATCAG CAAACCCCCC ATGGGCACTT GGATGGGCCT ATTGAACAAC 
AAAGTTGGCA CTTTCAAATT TATCTATGTG GATGTGTTAA ATGAAGAGGA AGAGAAGCCG 
AAGCGACCCA CCAGGAGACG CCGAAAAAGC AGACCACCCC AACCCAAGTC AGTTGAGGAT 
CTCTTGGATC GCATCAACTT AAAGGAGCAC ATGCCCACTT TTCTGTTCAA TGGTTATGAA 
GATCTGGATA CCTTTAAGCT TTTAGAAGAA GAGGATTTGG ATGAACTGAA CATCCGAGAT 
CCTGAGCACA GAGCTGTTCT CCTCACAGCC GTGGAACATC TACAGGAGTA TGATAGTAAC 
AGCGACCAGT CAGGATCTCA GGAGAAACTT CTCATTGAAG GTCAAGGCCT AGCTGGATGC 
TCTCCTCGGG ATTCTGGCTG CTATGAAAGC AGTGAAAACC TGGAGAATGG TAAAACCCGA 
AGGACCTGTC TTCTGCCCTC AAAATCATCA AGTGAGCATA GCTTTAAAGA CTTCAGCAGA 
CACCAGCTAT CAAATTATCC CACACTCCCA CTGACCAAGT CAATAGAAAC TCTACAGCAA 
GGAGAAAAAG AAAGCCGGTT GAGCTGTGCA CATCGTGCTC TGAAGAGCTC TGTCAAGACC 
CCAGCTGTTA TGGGTCTGAA GAAGAACCGT AGAAGTTTAC CAGTTGCTGT CTGTCGGAGC 
TATGAAACTC TGGATGGCCC CCAGAGTGTG GATGTATGGC CAAGATCTCA CTCCCTGGAC 
GATCTCCAGG GAGATTCCGA TACTAATCTA CAAGATGGTA GCAAGAAAGA TTATTTTGCT 
CGGGATTCAC TGGATACAGC GAAAAAGGCA ACTGGATCTG CCCTGCCACC TCAGTCGTGT 
GGGGGCAGTT GTATGGTCGA TAACTTGATG CCTAAGCAGG GTAAAGCAGC TGCTGGTTCT 
CAGAAGGGTA GAGGTAAGGA GTTGGAGTGC TCTGTAATGG AGAGTGCAGT TGGGAAACCT 
TTTCCATTCC CCATGAAACA CTGTGAAGCT CAGGCTGCCT TGGTGACACA TTCTGCAACA 
AGGACACCTC TGGAAATACA GAGTAAGGGC TTTCATGACC TTGCACGGGC TGACTATGCT 
CCAGTCCTCA AGGGAGGTCT AGAAGCTGAG CAAAAAGGCA CAAATGAGAC AAGAATGCAA 
CCAAAAAATC CCTCTCAGCC GCCACCTGTT CCTGCCAAAA AATGCCGAGA ACGCCTTACT 
AATGGATTAT ATCATCCTCC CATGACCCCC AGTGGGAACA ACTCAAGTCA AGAAGCACCG 
TGTTTGCCAG TAAAAAAGAC CAGTGCATCC AATCCCACTG ATTTTCATGG AGTACCTGTC 
CATAGGACAT CTGAACAGGA GCCCAGTACC CCTCCTAGCT CACTGCCACC CTGGCTGTTG 
GAGCAACCAG AGACTAAAAG CGTTCAGCAG CACGTGATAA AGCTAGGGCC TGCTTCAGCA 
AGGAAAGTCT CTTGTAGCAG GGGAATGGAT CTGGAAATGG TAATAGAAAA CAAGTTGCAG 
TCTGAAGGTA TTGATCTTAC TGAAGAACCA TACTCAGACA AGCATGGTCG CTGTGGCATT 
CCTGAGGCTT TGGTACAGAG GTACTCCGAA GACTTAGACC AGCCTGAAAA GGATGTTGCT 
GCAAACATGG ACCAAATCCG GGTAAAGCAG CTGAGGAAGC AGCATCGCAT GGCAATTCCA 
AGTGGAGGGC TCACTGAAAT TTGCCGAAAA CCTGTATCAC CAGGACTTAT CACCTCCGTA 
TCTGACTGGC TGATTTCCAT TGGCCTACCC ATGTATTCCA GCCCACTCAC AGAAGCAGGG 
TTCAACACAC TGAGCAAAGT GCCTTCTCTG TCACAAACTT GCCTTCAGGA AGCTGGCATC 
ACAGAGGAAA GGCACATAAG CAAGCTCCTG GCAGCAGCAA GACTCTTCAA ACCTCTCGAT 
CCAGACGCAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005145.1
CDS	139..3153
Translation	MPKLSREQSDDETEESVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGVEEQSLDNSAILDDRSALYSGVHKKQFYFDGSSEKQTEDDSDSLTTSPSSSSLDTWGASRKLVKTFSKTDSRGLIKPPKKLGTFFSYPEDEKTQKVCRSLTDGEMKKSLGSLSHGRTCSFGGFDLTNRSLHIGNNSDQMGKEGDFVYKEVIKSPSASRISLGKKVKSVKETMKKRMSKKYSSSLSEQESSPGVIPGSPQSPPPDTDSLDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRRKSRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVEHLQEYDSNSDQSGSQEKLLIEGQGLAGCSPRDSGCYESSENLENGKTRRTCLLPSKSSSEHSFKDFSRHQLSNYPTLPLTKSIETLQQGEKESRLSCAHRALKSSVKTPAVMGLKKNRRSLPVAVCRSYETLDGPQSVDVWPRSHSLDDLQGDSDTNLQDGSKKDYFARDSLDTAKKATGSALPPQSCGGSCMVDNLMPKQGKAAAGSQKGRGKELECSVMESAVGKPFPFPMKHCEAQAALVTHSATRTPLEIQSKGFHDLARADYAPVLKGGLEAEQKGTNETRMQPKNPSQPPPVPAKKCRERLTNGLYHPPMTPSGNNSSQEAPCLPVKKTSASNPTDFHGVPVHRTSEQEPSTPPSSLPPWLLEQPETKSVQQHVIKLGPASARKVSCSRGMDLEMVIENKLQSEGIDLTEEPYSDKHGRCGIPEALVQRYSEDLDQPEKDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGLITSVSDWLISIGLPMYSSPLTEAGFNTLSKVPSLSQTCLQEAGITEERHISKLLAAARLFKPLDPDAI

Target ORF information:

RefSeq Version	XM_025149377.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149377.1

ORF Insert Sequence:

ATGCCGAAGC TGTCAAGAGA ACAATCCGAT GATGAAACAG AGGAGTCGGT GAAGTTTAAG 
AGATTGCACA AGCTGGTAAA TTCTACTCGC AGAGTCAGGA AGAAACTCAT AAGGGTGGAA 
GAAATGAAAA AACCCAGTAC AGAAGGCGTA GAGGAGCAGT CGCTTGACAA CTCTGCTATA 
CTGGATGACA GATCGGCACT TTACTCTGGA GTTCACAAGA AGCAATTCTA CTTTGATGGT 
TCCTCCGAGA AGCAGACAGA GGATGACTCG GACTCACTTA CTACTTCTCC CTCATCTAGT 
AGTCTTGATA CATGGGGAGC TAGCCGGAAA CTGGTGAAGA CCTTCAGCAA AACTGATAGC 
CGTGGCCTTA TCAAGCCTCC CAAGAAACTT GGGACATTTT TTTCTTACCC AGAAGATGAG 
AAGACCCAGA AAGTTTGCCG CTCCTTGACA GATGGGGAGA TGAAGAAGAG CCTCGGCTCA 
CTGAGCCATG GGAGAACCTG CAGCTTTGGA GGATTTGACT TGACAAATCG TTCCCTTCAT 
ATTGGAAACA ATTCTGACCA AATGGGCAAA GAAGGTGACT TTGTTTATAA AGAAGTAATC 
AAATCACCCT CTGCCTCCCG TATATCTCTG GGCAAAAAGG TGAAATCTGT GAAAGAAACC 
ATGAAGAAAA GGATGTCTAA AAAATACAGC AGCTCTCTGT CTGAGCAGGA GTCGAGCCCT 
GGAGTCATAC CGGGTTCTCC GCAGTCGCCG CCGCCAGACA CTGACTCTCT GGACAAGCCC 
AAGCTGAAAG CTGGTGGCTC AGTTGAGAGC CTGAGGAGTT CCTTAAGTGG TCAGAGCTCA 
ATGAGTGGTC AGACAGTGAG TACAACAGAT TCCTCAACCA GCAACAGGGA GAGCGTGAAA 
TCAGAAGATG GGGATGATGA AGAACCTCCC TACAGAGGAC CCTTCTGTGG AAGAGCCAGG 
GTTCACACTG ATTTTACTCC AAGTCCTTAT GATACAGACT CTCTGAAGCT CAAGAAAGGT 
GATATCATTG ACATCATCAG CAAACCCCCC ATGGGCACTT GGATGGGCCT ATTGAACAAC 
AAAGTTGGCA CTTTCAAATT TATCTATGTG GATGTGTTAA ATGAAGAGGA AGAGAAGCCG 
AAGCGACCCA CCAGGAGACG CCGAAAAAGC AGACCACCCC AACCCAAGTC AGTTGAGGAT 
CTCTTGGATC GCATCAACTT AAAGGAGCAC ATGCCCACTT TTCTGTTCAA TGGTTATGAA 
GATCTGGATA CCTTTAAGCT TTTAGAAGAA GAGGATTTGG ATGAACTGAA CATCCGAGAT 
CCTGAGCACA GAGCTGTTCT CCTCACAGCC GTGGAACATC TACAGGAGTA TGATAGTAAC 
AGCGACCAGT CAGGATCTCA GGAGAAACTT CTCATTGAAG GTCAAGGCCT AGCTGGATGC 
TCTCCTCGGG ATTCTGGCTG CTATGAAAGC AGTGAAAACC TGGAGAATGG TAAAACCCGA 
AGGACCTGTC TTCTGCCCTC AAAATCATCA AGTGAGCATA GCTTTAAAGA CTTCAGCAGA 
CACCAGCTAT CAAATTATCC CACACTCCCA CTGACCAAGT CAATAGAAAC TCTACAGCAA 
GGAGAAAAAG AAAGCCGGTT GAGCTGTGCA CATCGTGCTC TGAAGAGCTC TGTCAAGACC 
CCAGCTGTTA TGGGTCTGAA GAAGAACCGT AGAAGTTTAC CAGTTGCTGT CTGTCGGAGC 
TATGAAACTC TGGATGGCCC CCAGAGTGTG GATGTATGGC CAAGATCTCA CTCCCTGGAC 
GATCTCCAGG GAGATTCCGA TACTAATCTA CAAGATGGTA GCAAGAAAGA TTATTTTGCT 
CGGGATTCAC TGGATACAGC GAAAAAGGCA ACTGGATCTG CCCTGCCACC TCAGTCGTGT 
GGGGGCAGTT GTATGGTCGA TAACTTGATG CCTAAGCAGG GTAAAGCAGC TGCTGGTTCT 
CAGAAGGGTA GAGGTAAGGA GTTGGAGTGC TCTGTAATGG AGAGTGCAGT TGGGAAACCT 
TTTCCATTCC CCATGAAACA CTGTGAAGCT CAGGCTGCCT TGGTGACACA TTCTGCAACA 
AGGACACCTC TGGAAATACA GAGTAAGGGC TTTCATGACC TTGCACGGGC TGACTATGCT 
CCAGTCCTCA AGGGAGGTCT AGAAGCTGAG CAAAAAGGCA CAAATGAGAC AAGAATGCAA 
CCAAAAAATC CCTCTCAGCC GCCACCTGTT CCTGCCAAAA AATGCCGAGA ACGCCTTACT 
AATGGATTAT ATCATCCTCC CATGACCCCC AGTGGGAACA ACTCAAGTCA AGAAGCACCG 
TGTTTGCCAG TAAAAAAGAC CAGTGCATCC AATCCCACTG ATTTTCATGG AGTACCTGTC 
CATAGGACAT CTGAACAGGA GCCCAGTACC CCTCCTAGCT CACTGCCACC CTGGCTGTTG 
GAGCAACCAG AGACTAAAAG CGTTCAGCAG CACGTGATAA AGCTAGGGCC TGCTTCAGCA 
AGGAAAGTCT CTTGTAGCAG GGGAATGGAT CTGGAAATGG TAATAGAAAA CAAGTTGCAG 
TCTGAAGGTA TTGATCTTAC TGAAGAACCA TACTCAGACA AGCATGGTCG CTGTGGCATT 
CCTGAGGCTT TGGTACAGAG GTACTCCGAA GACTTAGACC AGCCTGAAAA GGATGTTGCT 
GCAAACATGG ACCAAATCCG GGTAAAGCAG CTGAGGAAGC AGCATCGCAT GGCAATTCCA 
AGTGGAGGGC TCACTGAAAT TTGCCGAAAA CCTGTATCAC CAGGACTTAT CACCTCCGTA 
TCTGACTGGC TGATTTCCAT TGGCCTACCC ATGTATTCCA GCCCACTCAC AGAAGCAGGG 
TTCAACACAC TGAGCAAAGT GCCTTCTCTG TCACAAACTT GCCTTCAGGA AGCTGGCATC 
ACAGAGGAAA GGCACATAAG CAAGCTCCTG GCAGCAGCAA GACTCTTCAA ACCTCTCGAT 
CCAGACGCAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH1 ( XM_015284278.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139764.1 Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X4, mRNA.

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CloneID	OGa33753
Clone ID Related Accession (Same CDS sequence)	XM_015284278.1
Accession Version	XM_015284278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	SAM and SH3 domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGA CGCTTCTACA GCCCTTCCCC AAGGAGCGCC GTGCCTCACG CGCAACCGCT 
GCCGGGCCTC CTTCCCCCCG GAGCGCGGAG CCCCGGCAGC GGCCGCAGGA TGGGTCTCTG 
GGAAACATTG ATGACCTCGC ACAGCAGTAT GCAGATTATT ACAACACCTG CTTCACGGAT 
GTGTGTGAGA GGATGGAAGA GCTGCGCAAA CGGAGGGTTT CCCAGGACCT TGATGTGGAG 
AAACCTGATG CCAGTCCCAC ATCTCTACAA CTGCGATCCC AGATTGAGGA GTCACTTGGT 
CTCAGCAGCA CCGCATCAAC ACCTGACACT GAAAGAAAGC TCTCCATTCA TAAATCAAGT 
TCGGAAGAAG GCTCTGTAGG GAAAGCAGAC TGGAAAAAGA AAAATAAGTT TTTTTGGCAA 
AATTTCCGAA AGAACCAGAA AGGATTGATG AGGCAGACTT CAAAAGGAGA GGATGTGGGG 
TATGTGGCCA GTGAGATTAC GATGAGCGAT GAGGAACGGA TCCAGCTGAT GATGATGGTG 
AAAGAGAAGA TGATCACTAT TGAGGAAGCA CTTGCTAGGC TGAAGGAGTA TGAGTCCCAG 
CACAGGCAAT CAAGTACTGT AGATGCTACA GACTGGCCTG ATGGTTCTTA CTCAACATTT 
GATGGGTCTT CCAATTGTAA TTCAAGAGAA CAATCCGATG ATGAAACAGA GGAGTCGGTG 
AAGTTTAAGA GATTGCACAA GCTGGTAAAT TCTACTCGCA GAGTCAGGAA GAAACTCATA 
AGGGTGGAAG AAATGAAAAA ACCCAGTACA GAAGGCGTAG AGGAGCAGTC GCTTGACAAC 
TCTGCTATAC TGGATGACAG ATCGGCACTT TACTCTGGAG TTCACAAGAA GCAATTCTAC 
TTTGATGGTT CCTCCGAGAA GCAGACAGAG GATGACTCGG ACTCACTTAC TACTTCTCCC 
TCATCTAGTA GTCTTGATAC ATGGGGAGCT AGCCGGAAAC TGGTGAAGAC CTTCAGCAAA 
ACTGATAGCC GTGGCCTTAT CAAGCCTCCC AAGAAACTTG GGACATTTTT TTCTTACCCA 
GAAGATGAGA AGACCCAGAA AGTTTGCCGC TCCTTGACAG ATGGGGAGAT GAAGAAGAGC 
CTCGGCTCAC TGAGCCATGG GAGAACCTGC AGCTTTGGAG GATTTGACTT GACAAATCGT 
TCCCTTCATA TTGGAAACAA TTCTGACCAA ATGGGCAAAG AAGGTGACTT TGTTTATAAA 
GAAGTAATCA AATCACCCTC TGCCTCCCGT ATATCTCTGG GCAAAAAGGT GAAATCTGTG 
AAAGAAACCA TGAAGAAAAG GATGTCTAAA AAATACAGCA GCTCTCTGTC TGAGCAGGAG 
TCGAGCCCTG GAGTCATACC GGGTTCTCCG CAGTCGCCGC CGCCAGACAC TGACTCTCTG 
GACAAGCCCA AGCTGAAAGC TGGTGGCTCA GTTGAGAGCC TGAGGAGTTC CTTAAGTGGT 
CAGAGCTCAA TGAGTGGTCA GACAGTGAGT ACAACAGATT CCTCAACCAG CAACAGGGAG 
AGCGTGAAAT CAGAAGATGG GGATGATGAA GAACCTCCCT ACAGAGGACC CTTCTGTGGA 
AGAGCCAGGG TTCACACTGA TTTTACTCCA AGTCCTTATG ATACAGACTC TCTGAAGCTC 
AAGAAAGGTG ATATCATTGA CATCATCAGC AAACCCCCCA TGGGCACTTG GATGGGCCTA 
TTGAACAACA AAGTTGGCAC TTTCAAATTT ATCTATGTGG ATGTGTTAAA TGAAGAGGAA 
GAGAAGCCGA AGCGACCCAC CAGGAGACGC CGAAAAAGCA GACCACCCCA ACCCAAGTCA 
GTTGAGGATC TCTTGGATCG CATCAACTTA AAGGAGCACA TGCCCACTTT TCTGTTCAAT 
GGTTATGAAG ATCTGGATAC CTTTAAGCTT TTAGAAGAAG AGGATTTGGA TGAACTGAAC 
ATCCGAGATC CTGAGCACAG AGCTGTTCTC CTCACAGCCG TGGAACATCT ACAGGAGTAT 
GATAGTAACA GCGACCAGTC AGGATCTCAG GAGAAACTTC TCATTGAAGG TCAAGGCCTA 
GCTGGATGCT CTCCTCGGGA TTCTGGCTGC TATGAAAGCA GTGAAAACCT GGAGAATGGT 
AAAACCCGAA GGACCTGTCT TCTGCCCTCA AAATCATCAA GTGAGCATAG CTTTAAAGAC 
TTCAGCAGAC ACCAGCTATC AAATTATCCC ACACTCCCAC TGACCAAGTC AATAGAAACT 
CTACAGCAAG GAGAAAAAGA AAGCCGGTTG AGCTGTGCAC ATCGTGCTCT GAAGAGCTCT 
GTCAAGACCC CAGCTGTTAT GGGTCTGAAG AAGAACCGTA GAAGTTTACC AGTTGCTGTC 
TGTCGGAGCT ATGAAACTCT GGATGGCCCC CAGAGTGTGG ATGTATGGCC AAGATCTCAC 
TCCCTGGACG ATCTCCAGGG AGATTCCGAT ACTAATCTAC AAGATGGTAG CAAGAAAGAT 
TATTTTGCTC GGGATTCACT GGATACAGCG AAAAAGGCAA CTGGATCTGC CCTGCCACCT 
CAGTCGTGTG GGGGCAGTTG TATGGTCGAT AACTTGATGC CTAAGCAGGG TAAAGCAGCT 
GCTGGTTCTC AGAAGGGTAG AGGTAAGGAG TTGGAGTGCT CTGTAATGGA GAGTGCAGTT 
GGGAAACCTT TTCCATTCCC CATGAAACAC TGTGAAGCTC AGGCTGCCTT GGTGACACAT 
TCTGCAACAA GGACACCTCT GGAAATACAG AGTAAGGGCT TTCATGACCT TGCACGGGCT 
GACTATGCTC CAGTCCTCAA GGGAGGTCTA GAAGCTGAGC AAAAAGGCAC AAATGAGACA 
AGAATGCAAC CAAAAAATCC CTCTCAGCCG CCACCTGTTC CTGCCAAAAA ATGCCGAGAA 
CGCCTTACTA ATGGATTATA TCATCCTCCC ATGACCCCCA GTGGGAACAA CTCAAGTCAA 
GAAGCACCGT GTTTGCCAGT AAAAAAGACC AGTGCATCCA ATCCCACTGA TTTTCATGGA 
GTACCTGTCC ATAGGACATC TGAACAGGAG CCCAGTACCC CTCCTAGCTC ACTGCCACCC 
TGGCTGTTGG AGCAACCAGA GACTAAAAGC GTTCAGCAGC ACGTGATAAA GCTAGGGCCT 
GCTTCAGCAA GGAAAGTCTC TTGTAGCAGG GGAATGGATC TGGAAATGGT AATAGAAAAC 
AAGTTGCAGT CTGAAGGTAT TGATCTTACT GAAGAACCAT ACTCAGACAA GCATGGTCGC 
TGTGGCATTC CTGAGGCTTT GGTACAGAGG TACTCCGAAG ACTTAGACCA GCCTGAAAAG 
GATGTTGCTG CAAACATGGA CCAAATCCGG GTAAAGCAGC TGAGGAAGCA GCATCGCATG 
GCAATTCCAA GTGGAGGGCT CACTGAAATT TGCCGAAAAC CTGTATCACC AGGACTTATC 
ACCTCCGTAT CTGACTGGCT GATTTCCATT GGCCTACCCA TGTATTCCAG CCCACTCACA 
GAAGCAGGGT TCAACACACT GAGCAAAGTG CCTTCTCTGT CACAAACTTG CCTTCAGGAA 
GCTGGCATCA CAGAGGAAAG GCACATAAGC AAGCTCCTGG CAGCAGCAAG ACTCTTCAAA 
CCTCTCGATC CAGACGCAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139764.1
CDS	16..3699
Translation	MEATLLQPFPKERRASRATAAGPPSPRSAEPRQRPQDGSLGNIDDLAQQYADYYNTCFTDVCERMEELRKRRVSQDLDVEKPDASPTSLQLRSQIEESLGLSSTASTPDTERKLSIHKSSSEEGSVGKADWKKKNKFFWQNFRKNQKGLMRQTSKGEDVGYVASEITMSDEERIQLMMMVKEKMITIEEALARLKEYESQHRQSSTVDATDWPDGSYSTFDGSSNCNSREQSDDETEESVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGVEEQSLDNSAILDDRSALYSGVHKKQFYFDGSSEKQTEDDSDSLTTSPSSSSLDTWGASRKLVKTFSKTDSRGLIKPPKKLGTFFSYPEDEKTQKVCRSLTDGEMKKSLGSLSHGRTCSFGGFDLTNRSLHIGNNSDQMGKEGDFVYKEVIKSPSASRISLGKKVKSVKETMKKRMSKKYSSSLSEQESSPGVIPGSPQSPPPDTDSLDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRRKSRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVEHLQEYDSNSDQSGSQEKLLIEGQGLAGCSPRDSGCYESSENLENGKTRRTCLLPSKSSSEHSFKDFSRHQLSNYPTLPLTKSIETLQQGEKESRLSCAHRALKSSVKTPAVMGLKKNRRSLPVAVCRSYETLDGPQSVDVWPRSHSLDDLQGDSDTNLQDGSKKDYFARDSLDTAKKATGSALPPQSCGGSCMVDNLMPKQGKAAAGSQKGRGKELECSVMESAVGKPFPFPMKHCEAQAALVTHSATRTPLEIQSKGFHDLARADYAPVLKGGLEAEQKGTNETRMQPKNPSQPPPVPAKKCRERLTNGLYHPPMTPSGNNSSQEAPCLPVKKTSASNPTDFHGVPVHRTSEQEPSTPPSSLPPWLLEQPETKSVQQHVIKLGPASARKVSCSRGMDLEMVIENKLQSEGIDLTEEPYSDKHGRCGIPEALVQRYSEDLDQPEKDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGLITSVSDWLISIGLPMYSSPLTEAGFNTLSKVPSLSQTCLQEAGITEERHISKLLAAARLFKPLDPDAI

Target ORF information:

RefSeq Version	XM_015284278.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015284278.1

ORF Insert Sequence:

ATGGAGGCGA CGCTTCTACA GCCCTTCCCC AAGGAGCGCC GTGCCTCACG CGCAACCGCT 
GCCGGGCCTC CTTCCCCCCG GAGCGCGGAG CCCCGGCAGC GGCCGCAGGA TGGGTCTCTG 
GGAAACATTG ATGACCTCGC ACAGCAGTAT GCAGATTATT ACAACACCTG CTTCACGGAT 
GTGTGTGAGA GGATGGAAGA GCTGCGCAAA CGGAGGGTTT CCCAGGACCT TGATGTGGAG 
AAACCTGATG CCAGTCCCAC ATCTCTACAA CTGCGATCCC AGATTGAGGA GTCACTTGGT 
CTCAGCAGCA CCGCATCAAC ACCTGACACT GAAAGAAAGC TCTCCATTCA TAAATCAAGT 
TCGGAAGAAG GCTCTGTAGG GAAAGCAGAC TGGAAAAAGA AAAATAAGTT TTTTTGGCAA 
AATTTCCGAA AGAACCAGAA AGGATTGATG AGGCAGACTT CAAAAGGAGA GGATGTGGGG 
TATGTGGCCA GTGAGATTAC GATGAGCGAT GAGGAACGGA TCCAGCTGAT GATGATGGTG 
AAAGAGAAGA TGATCACTAT TGAGGAAGCA CTTGCTAGGC TGAAGGAGTA TGAGTCCCAG 
CACAGGCAAT CAAGTACTGT AGATGCTACA GACTGGCCTG ATGGTTCTTA CTCAACATTT 
GATGGGTCTT CCAATTGTAA TTCAAGAGAA CAATCCGATG ATGAAACAGA GGAGTCGGTG 
AAGTTTAAGA GATTGCACAA GCTGGTAAAT TCTACTCGCA GAGTCAGGAA GAAACTCATA 
AGGGTGGAAG AAATGAAAAA ACCCAGTACA GAAGGCGTAG AGGAGCAGTC GCTTGACAAC 
TCTGCTATAC TGGATGACAG ATCGGCACTT TACTCTGGAG TTCACAAGAA GCAATTCTAC 
TTTGATGGTT CCTCCGAGAA GCAGACAGAG GATGACTCGG ACTCACTTAC TACTTCTCCC 
TCATCTAGTA GTCTTGATAC ATGGGGAGCT AGCCGGAAAC TGGTGAAGAC CTTCAGCAAA 
ACTGATAGCC GTGGCCTTAT CAAGCCTCCC AAGAAACTTG GGACATTTTT TTCTTACCCA 
GAAGATGAGA AGACCCAGAA AGTTTGCCGC TCCTTGACAG ATGGGGAGAT GAAGAAGAGC 
CTCGGCTCAC TGAGCCATGG GAGAACCTGC AGCTTTGGAG GATTTGACTT GACAAATCGT 
TCCCTTCATA TTGGAAACAA TTCTGACCAA ATGGGCAAAG AAGGTGACTT TGTTTATAAA 
GAAGTAATCA AATCACCCTC TGCCTCCCGT ATATCTCTGG GCAAAAAGGT GAAATCTGTG 
AAAGAAACCA TGAAGAAAAG GATGTCTAAA AAATACAGCA GCTCTCTGTC TGAGCAGGAG 
TCGAGCCCTG GAGTCATACC GGGTTCTCCG CAGTCGCCGC CGCCAGACAC TGACTCTCTG 
GACAAGCCCA AGCTGAAAGC TGGTGGCTCA GTTGAGAGCC TGAGGAGTTC CTTAAGTGGT 
CAGAGCTCAA TGAGTGGTCA GACAGTGAGT ACAACAGATT CCTCAACCAG CAACAGGGAG 
AGCGTGAAAT CAGAAGATGG GGATGATGAA GAACCTCCCT ACAGAGGACC CTTCTGTGGA 
AGAGCCAGGG TTCACACTGA TTTTACTCCA AGTCCTTATG ATACAGACTC TCTGAAGCTC 
AAGAAAGGTG ATATCATTGA CATCATCAGC AAACCCCCCA TGGGCACTTG GATGGGCCTA 
TTGAACAACA AAGTTGGCAC TTTCAAATTT ATCTATGTGG ATGTGTTAAA TGAAGAGGAA 
GAGAAGCCGA AGCGACCCAC CAGGAGACGC CGAAAAAGCA GACCACCCCA ACCCAAGTCA 
GTTGAGGATC TCTTGGATCG CATCAACTTA AAGGAGCACA TGCCCACTTT TCTGTTCAAT 
GGTTATGAAG ATCTGGATAC CTTTAAGCTT TTAGAAGAAG AGGATTTGGA TGAACTGAAC 
ATCCGAGATC CTGAGCACAG AGCTGTTCTC CTCACAGCCG TGGAACATCT ACAGGAGTAT 
GATAGTAACA GCGACCAGTC AGGATCTCAG GAGAAACTTC TCATTGAAGG TCAAGGCCTA 
GCTGGATGCT CTCCTCGGGA TTCTGGCTGC TATGAAAGCA GTGAAAACCT GGAGAATGGT 
AAAACCCGAA GGACCTGTCT TCTGCCCTCA AAATCATCAA GTGAGCATAG CTTTAAAGAC 
TTCAGCAGAC ACCAGCTATC AAATTATCCC ACACTCCCAC TGACCAAGTC AATAGAAACT 
CTACAGCAAG GAGAAAAAGA AAGCCGGTTG AGCTGTGCAC ATCGTGCTCT GAAGAGCTCT 
GTCAAGACCC CAGCTGTTAT GGGTCTGAAG AAGAACCGTA GAAGTTTACC AGTTGCTGTC 
TGTCGGAGCT ATGAAACTCT GGATGGCCCC CAGAGTGTGG ATGTATGGCC AAGATCTCAC 
TCCCTGGACG ATCTCCAGGG AGATTCCGAT ACTAATCTAC AAGATGGTAG CAAGAAAGAT 
TATTTTGCTC GGGATTCACT GGATACAGCG AAAAAGGCAA CTGGATCTGC CCTGCCACCT 
CAGTCGTGTG GGGGCAGTTG TATGGTCGAT AACTTGATGC CTAAGCAGGG TAAAGCAGCT 
GCTGGTTCTC AGAAGGGTAG AGGTAAGGAG TTGGAGTGCT CTGTAATGGA GAGTGCAGTT 
GGGAAACCTT TTCCATTCCC CATGAAACAC TGTGAAGCTC AGGCTGCCTT GGTGACACAT 
TCTGCAACAA GGACACCTCT GGAAATACAG AGTAAGGGCT TTCATGACCT TGCACGGGCT 
GACTATGCTC CAGTCCTCAA GGGAGGTCTA GAAGCTGAGC AAAAAGGCAC AAATGAGACA 
AGAATGCAAC CAAAAAATCC CTCTCAGCCG CCACCTGTTC CTGCCAAAAA ATGCCGAGAA 
CGCCTTACTA ATGGATTATA TCATCCTCCC ATGACCCCCA GTGGGAACAA CTCAAGTCAA 
GAAGCACCGT GTTTGCCAGT AAAAAAGACC AGTGCATCCA ATCCCACTGA TTTTCATGGA 
GTACCTGTCC ATAGGACATC TGAACAGGAG CCCAGTACCC CTCCTAGCTC ACTGCCACCC 
TGGCTGTTGG AGCAACCAGA GACTAAAAGC GTTCAGCAGC ACGTGATAAA GCTAGGGCCT 
GCTTCAGCAA GGAAAGTCTC TTGTAGCAGG GGAATGGATC TGGAAATGGT AATAGAAAAC 
AAGTTGCAGT CTGAAGGTAT TGATCTTACT GAAGAACCAT ACTCAGACAA GCATGGTCGC 
TGTGGCATTC CTGAGGCTTT GGTACAGAGG TACTCCGAAG ACTTAGACCA GCCTGAAAAG 
GATGTTGCTG CAAACATGGA CCAAATCCGG GTAAAGCAGC TGAGGAAGCA GCATCGCATG 
GCAATTCCAA GTGGAGGGCT CACTGAAATT TGCCGAAAAC CTGTATCACC AGGACTTATC 
ACCTCCGTAT CTGACTGGCT GATTTCCATT GGCCTACCCA TGTATTCCAG CCCACTCACA 
GAAGCAGGGT TCAACACACT GAGCAAAGTG CCTTCTCTGT CACAAACTTG CCTTCAGGAA 
GCTGGCATCA CAGAGGAAAG GCACATAAGC AAGCTCCTGG CAGCAGCAAG ACTCTTCAAA 
CCTCTCGATC CAGACGCAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH1 ( XM_419659.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_419659.5 Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa33754
Clone ID Related Accession (Same CDS sequence)	XM_419659.5
Accession Version	XM_419659.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	SAM and SH3 domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_419659.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGG AGCGGGGCCC GGGCGGCCCG TCGGAGAGCG GCCCGTCGGA GGCGTTCGCG 
CAGCTGTGGG CCGACGTGAT GGGCATTCTG GATGGGTCTC TGGGAAACAT TGATGACCTC 
GCACAGCAGT ATGCAGATTA TTACAACACC TGCTTCACGG ATGTGTGTGA GAGGATGGAA 
GAGCTGCGCA AACGGAGGGT TTCCCAGGAC CTTGATGTGG AGAAACCTGA TGCCAGTCCC 
ACATCTCTAC AACTGCGATC CCAGATTGAG GAGTCACTTG GTCTCAGCAG CACCGCATCA 
ACACCTGACA CTGAAAGAAA GCTCTCCATT CATAAATCAA GTTCGGAAGA AGGCTCTGTA 
GGGAAAGCAG ACTGGAAAAA GAAAAATAAG TTTTTTTGGC AAAATTTCCG AAAGAACCAG 
AAAGGATTGA TGAGGCAGAC TTCAAAAGGA GAGGATGTGG GGTATGTGGC CAGTGAGATT 
ACGATGAGCG ATGAGGAACG GATCCAGCTG ATGATGATGG TGAAAGAGAA GATGATCACT 
ATTGAGGAAG CACTTGCTAG GCTGAAGGAG TATGAGTCCC AGCACAGGCA ATCAAGTACT 
GTAGATGCTA CAGACTGGCC TGATGGTTCT TACTCAACAT TTGATGGGTC TTCCAATTGT 
AATTCAAGAG AACAATCCGA TGATGAAACA GAGGAGTCGG TGAAGTTTAA GAGATTGCAC 
AAGCTGGTAA ATTCTACTCG CAGAGTCAGG AAGAAACTCA TAAGGGTGGA AGAAATGAAA 
AAACCCAGTA CAGAAGGCGT AGAGGAGCAG TCGCTTGACA ACTCTGCTAT ACTGGATGAC 
AGATCGGCAC TTTACTCTGG AGTTCACAAG AAGCAATTCT ACTTTGATGG TTCCTCCGAG 
AAGCAGACAG AGGATGACTC GGACTCACTT ACTACTTCTC CCTCATCTAG TAGTCTTGAT 
ACATGGGGAG CTAGCCGGAA ACTGGTGAAG ACCTTCAGCA AAACTGATAG CCGTGGCCTT 
ATCAAGCCTC CCAAGAAACT TGGGACATTT TTTTCTTACC CAGAAGATGA GAAGACCCAG 
AAAGTTTGCC GCTCCTTGAC AGATGGGGAG ATGAAGAAGA GCCTCGGCTC ACTGAGCCAT 
GGGAGAACCT GCAGCTTTGG AGGATTTGAC TTGACAAATC GTTCCCTTCA TATTGGAAAC 
AATTCTGACC AAATGGGCAA AGAAGGTGAC TTTGTTTATA AAGAAGTAAT CAAATCACCC 
TCTGCCTCCC GTATATCTCT GGGCAAAAAG GTGAAATCTG TGAAAGAAAC CATGAAGAAA 
AGGATGTCTA AAAAATACAG CAGCTCTCTG TCTGAGCAGG AGTCGAGCCC TGGAGTCATA 
CCGGGTTCTC CGCAGTCGCC GCCGCCAGAC ACTGACTCTC TGGACAAGCC CAAGCTGAAA 
GCTGGTGGCT CAGTTGAGAG CCTGAGGAGT TCCTTAAGTG GTCAGAGCTC AATGAGTGGT 
CAGACAGTGA GTACAACAGA TTCCTCAACC AGCAACAGGG AGAGCGTGAA ATCAGAAGAT 
GGGGATGATG AAGAACCTCC CTACAGAGGA CCCTTCTGTG GAAGAGCCAG GGTTCACACT 
GATTTTACTC CAAGTCCTTA TGATACAGAC TCTCTGAAGC TCAAGAAAGG TGATATCATT 
GACATCATCA GCAAACCCCC CATGGGCACT TGGATGGGCC TATTGAACAA CAAAGTTGGC 
ACTTTCAAAT TTATCTATGT GGATGTGTTA AATGAAGAGG AAGAGAAGCC GAAGCGACCC 
ACCAGGAGAC GCCGAAAAAG CAGACCACCC CAACCCAAGT CAGTTGAGGA TCTCTTGGAT 
CGCATCAACT TAAAGGAGCA CATGCCCACT TTTCTGTTCA ATGGTTATGA AGATCTGGAT 
ACCTTTAAGC TTTTAGAAGA AGAGGATTTG GATGAACTGA ACATCCGAGA TCCTGAGCAC 
AGAGCTGTTC TCCTCACAGC CGTGGAACAT CTACAGGAGT ATGATAGTAA CAGCGACCAG 
TCAGGATCTC AGGAGAAACT TCTCATTGAA GGTCAAGGCC TAGCTGGATG CTCTCCTCGG 
GATTCTGGCT GCTATGAAAG CAGTGAAAAC CTGGAGAATG GTAAAACCCG AAGGACCTGT 
CTTCTGCCCT CAAAATCATC AAGTGAGCAT AGCTTTAAAG ACTTCAGCAG ACACCAGCTA 
TCAAATTATC CCACACTCCC ACTGACCAAG TCAATAGAAA CTCTACAGCA AGGAGAAAAA 
GAAAGCCGGT TGAGCTGTGC ACATCGTGCT CTGAAGAGCT CTGTCAAGAC CCCAGCTGTT 
ATGGGTCTGA AGAAGAACCG TAGAAGTTTA CCAGTTGCTG TCTGTCGGAG CTATGAAACT 
CTGGATGGCC CCCAGAGTGT GGATGTATGG CCAAGATCTC ACTCCCTGGA CGATCTCCAG 
GGAGATTCCG ATACTAATCT ACAAGATGGT AGCAAGAAAG ATTATTTTGC TCGGGATTCA 
CTGGATACAG CGAAAAAGGC AACTGGATCT GCCCTGCCAC CTCAGTCGTG TGGGGGCAGT 
TGTATGGTCG ATAACTTGAT GCCTAAGCAG GGTAAAGCAG CTGCTGGTTC TCAGAAGGGT 
AGAGGTAAGG AGTTGGAGTG CTCTGTAATG GAGAGTGCAG TTGGGAAACC TTTTCCATTC 
CCCATGAAAC ACTGTGAAGC TCAGGCTGCC TTGGTGACAC ATTCTGCAAC AAGGACACCT 
CTGGAAATAC AGAGTAAGGG CTTTCATGAC CTTGCACGGG CTGACTATGC TCCAGTCCTC 
AAGGGAGGTC TAGAAGCTGA GCAAAAAGGC ACAAATGAGA CAAGAATGCA ACCAAAAAAT 
CCCTCTCAGC CGCCACCTGT TCCTGCCAAA AAATGCCGAG AACGCCTTAC TAATGGATTA 
TATCATCCTC CCATGACCCC CAGTGGGAAC AACTCAAGTC AAGAAGCACC GTGTTTGCCA 
GTAAAAAAGA CCAGTGCATC CAATCCCACT GATTTTCATG GAGTACCTGT CCATAGGACA 
TCTGAACAGG AGCCCAGTAC CCCTCCTAGC TCACTGCCAC CCTGGCTGTT GGAGCAACCA 
GAGACTAAAA GCGTTCAGCA GCACGTGATA AAGCTAGGGC CTGCTTCAGC AAGGAAAGTC 
TCTTGTAGCA GGGGAATGGA TCTGGAAATG GTAATAGAAA ACAAGTTGCA GTCTGAAGGT 
ATTGATCTTA CTGAAGAACC ATACTCAGAC AAGCATGGTC GCTGTGGCAT TCCTGAGGCT 
TTGGTACAGA GGTACTCCGA AGACTTAGAC CAGCCTGAAA AGGATGTTGC TGCAAACATG 
GACCAAATCC GGGTAAAGCA GCTGAGGAAG CAGCATCGCA TGGCAATTCC AAGTGGAGGG 
CTCACTGAAA TTTGCCGAAA ACCTGTATCA CCAGGACTTA TCACCTCCGT ATCTGACTGG 
CTGATTTCCA TTGGCCTACC CATGTATTCC AGCCCACTCA CAGAAGCAGG GTTCAACACA 
CTGAGCAAAG TGCCTTCTCT GTCACAAACT TGCCTTCAGG AAGCTGGCAT CACAGAGGAA 
AGGCACATAA GCAAGCTCCT GGCAGCAGCA AGACTCTTCA AACCTCTCGA TCCAGACGCA 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_419659.5
CDS	133..3798
Translation	MEGERGPGGPSESGPSEAFAQLWADVMGILDGSLGNIDDLAQQYADYYNTCFTDVCERMEELRKRRVSQDLDVEKPDASPTSLQLRSQIEESLGLSSTASTPDTERKLSIHKSSSEEGSVGKADWKKKNKFFWQNFRKNQKGLMRQTSKGEDVGYVASEITMSDEERIQLMMMVKEKMITIEEALARLKEYESQHRQSSTVDATDWPDGSYSTFDGSSNCNSREQSDDETEESVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGVEEQSLDNSAILDDRSALYSGVHKKQFYFDGSSEKQTEDDSDSLTTSPSSSSLDTWGASRKLVKTFSKTDSRGLIKPPKKLGTFFSYPEDEKTQKVCRSLTDGEMKKSLGSLSHGRTCSFGGFDLTNRSLHIGNNSDQMGKEGDFVYKEVIKSPSASRISLGKKVKSVKETMKKRMSKKYSSSLSEQESSPGVIPGSPQSPPPDTDSLDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRRKSRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVEHLQEYDSNSDQSGSQEKLLIEGQGLAGCSPRDSGCYESSENLENGKTRRTCLLPSKSSSEHSFKDFSRHQLSNYPTLPLTKSIETLQQGEKESRLSCAHRALKSSVKTPAVMGLKKNRRSLPVAVCRSYETLDGPQSVDVWPRSHSLDDLQGDSDTNLQDGSKKDYFARDSLDTAKKATGSALPPQSCGGSCMVDNLMPKQGKAAAGSQKGRGKELECSVMESAVGKPFPFPMKHCEAQAALVTHSATRTPLEIQSKGFHDLARADYAPVLKGGLEAEQKGTNETRMQPKNPSQPPPVPAKKCRERLTNGLYHPPMTPSGNNSSQEAPCLPVKKTSASNPTDFHGVPVHRTSEQEPSTPPSSLPPWLLEQPETKSVQQHVIKLGPASARKVSCSRGMDLEMVIENKLQSEGIDLTEEPYSDKHGRCGIPEALVQRYSEDLDQPEKDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGLITSVSDWLISIGLPMYSSPLTEAGFNTLSKVPSLSQTCLQEAGITEERHISKLLAAARLFKPLDPDAI

Target ORF information:

RefSeq Version	XM_419659.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_419659.5

ORF Insert Sequence:

ATGGAGGGGG AGCGGGGCCC GGGCGGCCCG TCGGAGAGCG GCCCGTCGGA GGCGTTCGCG 
CAGCTGTGGG CCGACGTGAT GGGCATTCTG GATGGGTCTC TGGGAAACAT TGATGACCTC 
GCACAGCAGT ATGCAGATTA TTACAACACC TGCTTCACGG ATGTGTGTGA GAGGATGGAA 
GAGCTGCGCA AACGGAGGGT TTCCCAGGAC CTTGATGTGG AGAAACCTGA TGCCAGTCCC 
ACATCTCTAC AACTGCGATC CCAGATTGAG GAGTCACTTG GTCTCAGCAG CACCGCATCA 
ACACCTGACA CTGAAAGAAA GCTCTCCATT CATAAATCAA GTTCGGAAGA AGGCTCTGTA 
GGGAAAGCAG ACTGGAAAAA GAAAAATAAG TTTTTTTGGC AAAATTTCCG AAAGAACCAG 
AAAGGATTGA TGAGGCAGAC TTCAAAAGGA GAGGATGTGG GGTATGTGGC CAGTGAGATT 
ACGATGAGCG ATGAGGAACG GATCCAGCTG ATGATGATGG TGAAAGAGAA GATGATCACT 
ATTGAGGAAG CACTTGCTAG GCTGAAGGAG TATGAGTCCC AGCACAGGCA ATCAAGTACT 
GTAGATGCTA CAGACTGGCC TGATGGTTCT TACTCAACAT TTGATGGGTC TTCCAATTGT 
AATTCAAGAG AACAATCCGA TGATGAAACA GAGGAGTCGG TGAAGTTTAA GAGATTGCAC 
AAGCTGGTAA ATTCTACTCG CAGAGTCAGG AAGAAACTCA TAAGGGTGGA AGAAATGAAA 
AAACCCAGTA CAGAAGGCGT AGAGGAGCAG TCGCTTGACA ACTCTGCTAT ACTGGATGAC 
AGATCGGCAC TTTACTCTGG AGTTCACAAG AAGCAATTCT ACTTTGATGG TTCCTCCGAG 
AAGCAGACAG AGGATGACTC GGACTCACTT ACTACTTCTC CCTCATCTAG TAGTCTTGAT 
ACATGGGGAG CTAGCCGGAA ACTGGTGAAG ACCTTCAGCA AAACTGATAG CCGTGGCCTT 
ATCAAGCCTC CCAAGAAACT TGGGACATTT TTTTCTTACC CAGAAGATGA GAAGACCCAG 
AAAGTTTGCC GCTCCTTGAC AGATGGGGAG ATGAAGAAGA GCCTCGGCTC ACTGAGCCAT 
GGGAGAACCT GCAGCTTTGG AGGATTTGAC TTGACAAATC GTTCCCTTCA TATTGGAAAC 
AATTCTGACC AAATGGGCAA AGAAGGTGAC TTTGTTTATA AAGAAGTAAT CAAATCACCC 
TCTGCCTCCC GTATATCTCT GGGCAAAAAG GTGAAATCTG TGAAAGAAAC CATGAAGAAA 
AGGATGTCTA AAAAATACAG CAGCTCTCTG TCTGAGCAGG AGTCGAGCCC TGGAGTCATA 
CCGGGTTCTC CGCAGTCGCC GCCGCCAGAC ACTGACTCTC TGGACAAGCC CAAGCTGAAA 
GCTGGTGGCT CAGTTGAGAG CCTGAGGAGT TCCTTAAGTG GTCAGAGCTC AATGAGTGGT 
CAGACAGTGA GTACAACAGA TTCCTCAACC AGCAACAGGG AGAGCGTGAA ATCAGAAGAT 
GGGGATGATG AAGAACCTCC CTACAGAGGA CCCTTCTGTG GAAGAGCCAG GGTTCACACT 
GATTTTACTC CAAGTCCTTA TGATACAGAC TCTCTGAAGC TCAAGAAAGG TGATATCATT 
GACATCATCA GCAAACCCCC CATGGGCACT TGGATGGGCC TATTGAACAA CAAAGTTGGC 
ACTTTCAAAT TTATCTATGT GGATGTGTTA AATGAAGAGG AAGAGAAGCC GAAGCGACCC 
ACCAGGAGAC GCCGAAAAAG CAGACCACCC CAACCCAAGT CAGTTGAGGA TCTCTTGGAT 
CGCATCAACT TAAAGGAGCA CATGCCCACT TTTCTGTTCA ATGGTTATGA AGATCTGGAT 
ACCTTTAAGC TTTTAGAAGA AGAGGATTTG GATGAACTGA ACATCCGAGA TCCTGAGCAC 
AGAGCTGTTC TCCTCACAGC CGTGGAACAT CTACAGGAGT ATGATAGTAA CAGCGACCAG 
TCAGGATCTC AGGAGAAACT TCTCATTGAA GGTCAAGGCC TAGCTGGATG CTCTCCTCGG 
GATTCTGGCT GCTATGAAAG CAGTGAAAAC CTGGAGAATG GTAAAACCCG AAGGACCTGT 
CTTCTGCCCT CAAAATCATC AAGTGAGCAT AGCTTTAAAG ACTTCAGCAG ACACCAGCTA 
TCAAATTATC CCACACTCCC ACTGACCAAG TCAATAGAAA CTCTACAGCA AGGAGAAAAA 
GAAAGCCGGT TGAGCTGTGC ACATCGTGCT CTGAAGAGCT CTGTCAAGAC CCCAGCTGTT 
ATGGGTCTGA AGAAGAACCG TAGAAGTTTA CCAGTTGCTG TCTGTCGGAG CTATGAAACT 
CTGGATGGCC CCCAGAGTGT GGATGTATGG CCAAGATCTC ACTCCCTGGA CGATCTCCAG 
GGAGATTCCG ATACTAATCT ACAAGATGGT AGCAAGAAAG ATTATTTTGC TCGGGATTCA 
CTGGATACAG CGAAAAAGGC AACTGGATCT GCCCTGCCAC CTCAGTCGTG TGGGGGCAGT 
TGTATGGTCG ATAACTTGAT GCCTAAGCAG GGTAAAGCAG CTGCTGGTTC TCAGAAGGGT 
AGAGGTAAGG AGTTGGAGTG CTCTGTAATG GAGAGTGCAG TTGGGAAACC TTTTCCATTC 
CCCATGAAAC ACTGTGAAGC TCAGGCTGCC TTGGTGACAC ATTCTGCAAC AAGGACACCT 
CTGGAAATAC AGAGTAAGGG CTTTCATGAC CTTGCACGGG CTGACTATGC TCCAGTCCTC 
AAGGGAGGTC TAGAAGCTGA GCAAAAAGGC ACAAATGAGA CAAGAATGCA ACCAAAAAAT 
CCCTCTCAGC CGCCACCTGT TCCTGCCAAA AAATGCCGAG AACGCCTTAC TAATGGATTA 
TATCATCCTC CCATGACCCC CAGTGGGAAC AACTCAAGTC AAGAAGCACC GTGTTTGCCA 
GTAAAAAAGA CCAGTGCATC CAATCCCACT GATTTTCATG GAGTACCTGT CCATAGGACA 
TCTGAACAGG AGCCCAGTAC CCCTCCTAGC TCACTGCCAC CCTGGCTGTT GGAGCAACCA 
GAGACTAAAA GCGTTCAGCA GCACGTGATA AAGCTAGGGC CTGCTTCAGC AAGGAAAGTC 
TCTTGTAGCA GGGGAATGGA TCTGGAAATG GTAATAGAAA ACAAGTTGCA GTCTGAAGGT 
ATTGATCTTA CTGAAGAACC ATACTCAGAC AAGCATGGTC GCTGTGGCAT TCCTGAGGCT 
TTGGTACAGA GGTACTCCGA AGACTTAGAC CAGCCTGAAA AGGATGTTGC TGCAAACATG 
GACCAAATCC GGGTAAAGCA GCTGAGGAAG CAGCATCGCA TGGCAATTCC AAGTGGAGGG 
CTCACTGAAA TTTGCCGAAA ACCTGTATCA CCAGGACTTA TCACCTCCGT ATCTGACTGG 
CTGATTTCCA TTGGCCTACC CATGTATTCC AGCCCACTCA CAGAAGCAGG GTTCAACACA 
CTGAGCAAAG TGCCTTCTCT GTCACAAACT TGCCTTCAGG AAGCTGGCAT CACAGAGGAA 
AGGCACATAA GCAAGCTCCT GGCAGCAGCA AGACTCTTCA AACCTCTCGA TCCAGACGCA 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH1 ( XM_015284279.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139765.1 Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OGa33755
Clone ID Related Accession (Same CDS sequence)	XM_015284279.1 , XM_015284279.2
Accession Version	XM_015284279.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	SAM and SH3 domain-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC TGCGCAAACG GAGGGTTTCC CAGGACCTTG ATGTGGAGAA ACCTGATGCC 
AGTCCCACAT CTCTACAACT GCGATCCCAG ATTGAGGAGT CACTTGGTCT CAGCAGCACC 
GCATCAACAC CTGACACTGA AAGAAAGCTC TCCATTCATA AATCAAGTTC GGAAGAAGGC 
TCTGTAGGGA AAGCAGACTG GAAAAAGAAA AATAAGTTTT TTTGGCAAAA TTTCCGAAAG 
AACCAGAAAG GATTGATGAG GCAGACTTCA AAAGGAGAGG ATGTGGGGTA TGTGGCCAGT 
GAGATTACGA TGAGCGATGA GGAACGGATC CAGCTGATGA TGATGGTGAA AGAGAAGATG 
ATCACTATTG AGGAAGCACT TGCTAGGCTG AAGGAGTATG AGTCCCAGCA CAGGCAATCA 
AGTACTGTAG ATGCTACAGA CTGGCCTGAT GGTTCTTACT CAACATTTGA TGGGTCTTCC 
AATTGTAATT CAAGAGAACA ATCCGATGAT GAAACAGAGG AGTCGGTGAA GTTTAAGAGA 
TTGCACAAGC TGGTAAATTC TACTCGCAGA GTCAGGAAGA AACTCATAAG GGTGGAAGAA 
ATGAAAAAAC CCAGTACAGA AGGCGTAGAG GAGCAGTCGC TTGACAACTC TGCTATACTG 
GATGACAGAT CGGCACTTTA CTCTGGAGTT CACAAGAAGC AATTCTACTT TGATGGTTCC 
TCCGAGAAGC AGACAGAGGA TGACTCGGAC TCACTTACTA CTTCTCCCTC ATCTAGTAGT 
CTTGATACAT GGGGAGCTAG CCGGAAACTG GTGAAGACCT TCAGCAAAAC TGATAGCCGT 
GGCCTTATCA AGCCTCCCAA GAAACTTGGG ACATTTTTTT CTTACCCAGA AGATGAGAAG 
ACCCAGAAAG TTTGCCGCTC CTTGACAGAT GGGGAGATGA AGAAGAGCCT CGGCTCACTG 
AGCCATGGGA GAACCTGCAG CTTTGGAGGA TTTGACTTGA CAAATCGTTC CCTTCATATT 
GGAAACAATT CTGACCAAAT GGGCAAAGAA GGTGACTTTG TTTATAAAGA AGTAATCAAA 
TCACCCTCTG CCTCCCGTAT ATCTCTGGGC AAAAAGGTGA AATCTGTGAA AGAAACCATG 
AAGAAAAGGA TGTCTAAAAA ATACAGCAGC TCTCTGTCTG AGCAGGAGTC GAGCCCTGGA 
GTCATACCGG GTTCTCCGCA GTCGCCGCCG CCAGACACTG ACTCTCTGGA CAAGCCCAAG 
CTGAAAGCTG GTGGCTCAGT TGAGAGCCTG AGGAGTTCCT TAAGTGGTCA GAGCTCAATG 
AGTGGTCAGA CAGTGAGTAC AACAGATTCC TCAACCAGCA ACAGGGAGAG CGTGAAATCA 
GAAGATGGGG ATGATGAAGA ACCTCCCTAC AGAGGACCCT TCTGTGGAAG AGCCAGGGTT 
CACACTGATT TTACTCCAAG TCCTTATGAT ACAGACTCTC TGAAGCTCAA GAAAGGTGAT 
ATCATTGACA TCATCAGCAA ACCCCCCATG GGCACTTGGA TGGGCCTATT GAACAACAAA 
GTTGGCACTT TCAAATTTAT CTATGTGGAT GTGTTAAATG AAGAGGAAGA GAAGCCGAAG 
CGACCCACCA GGAGACGCCG AAAAAGCAGA CCACCCCAAC CCAAGTCAGT TGAGGATCTC 
TTGGATCGCA TCAACTTAAA GGAGCACATG CCCACTTTTC TGTTCAATGG TTATGAAGAT 
CTGGATACCT TTAAGCTTTT AGAAGAAGAG GATTTGGATG AACTGAACAT CCGAGATCCT 
GAGCACAGAG CTGTTCTCCT CACAGCCGTG GAACATCTAC AGGAGTATGA TAGTAACAGC 
GACCAGTCAG GATCTCAGGA GAAACTTCTC ATTGAAGGTC AAGGCCTAGC TGGATGCTCT 
CCTCGGGATT CTGGCTGCTA TGAAAGCAGT GAAAACCTGG AGAATGGTAA AACCCGAAGG 
ACCTGTCTTC TGCCCTCAAA ATCATCAAGT GAGCATAGCT TTAAAGACTT CAGCAGACAC 
CAGCTATCAA ATTATCCCAC ACTCCCACTG ACCAAGTCAA TAGAAACTCT ACAGCAAGGA 
GAAAAAGAAA GCCGGTTGAG CTGTGCACAT CGTGCTCTGA AGAGCTCTGT CAAGACCCCA 
GCTGTTATGG GTCTGAAGAA GAACCGTAGA AGTTTACCAG TTGCTGTCTG TCGGAGCTAT 
GAAACTCTGG ATGGCCCCCA GAGTGTGGAT GTATGGCCAA GATCTCACTC CCTGGACGAT 
CTCCAGGGAG ATTCCGATAC TAATCTACAA GATGGTAGCA AGAAAGATTA TTTTGCTCGG 
GATTCACTGG ATACAGCGAA AAAGGCAACT GGATCTGCCC TGCCACCTCA GTCGTGTGGG 
GGCAGTTGTA TGGTCGATAA CTTGATGCCT AAGCAGGGTA AAGCAGCTGC TGGTTCTCAG 
AAGGGTAGAG GTAAGGAGTT GGAGTGCTCT GTAATGGAGA GTGCAGTTGG GAAACCTTTT 
CCATTCCCCA TGAAACACTG TGAAGCTCAG GCTGCCTTGG TGACACATTC TGCAACAAGG 
ACACCTCTGG AAATACAGAG TAAGGGCTTT CATGACCTTG CACGGGCTGA CTATGCTCCA 
GTCCTCAAGG GAGGTCTAGA AGCTGAGCAA AAAGGCACAA ATGAGACAAG AATGCAACCA 
AAAAATCCCT CTCAGCCGCC ACCTGTTCCT GCCAAAAAAT GCCGAGAACG CCTTACTAAT 
GGATTATATC ATCCTCCCAT GACCCCCAGT GGGAACAACT CAAGTCAAGA AGCACCGTGT 
TTGCCAGTAA AAAAGACCAG TGCATCCAAT CCCACTGATT TTCATGGAGT ACCTGTCCAT 
AGGACATCTG AACAGGAGCC CAGTACCCCT CCTAGCTCAC TGCCACCCTG GCTGTTGGAG 
CAACCAGAGA CTAAAAGCGT TCAGCAGCAC GTGATAAAGC TAGGGCCTGC TTCAGCAAGG 
AAAGTCTCTT GTAGCAGGGG AATGGATCTG GAAATGGTAA TAGAAAACAA GTTGCAGTCT 
GAAGGTATTG ATCTTACTGA AGAACCATAC TCAGACAAGC ATGGTCGCTG TGGCATTCCT 
GAGGCTTTGG TACAGAGGTA CTCCGAAGAC TTAGACCAGC CTGAAAAGGA TGTTGCTGCA 
AACATGGACC AAATCCGGGT AAAGCAGCTG AGGAAGCAGC ATCGCATGGC AATTCCAAGT 
GGAGGGCTCA CTGAAATTTG CCGAAAACCT GTATCACCAG GACTTATCAC CTCCGTATCT 
GACTGGCTGA TTTCCATTGG CCTACCCATG TATTCCAGCC CACTCACAGA AGCAGGGTTC 
AACACACTGA GCAAAGTGCC TTCTCTGTCA CAAACTTGCC TTCAGGAAGC TGGCATCACA 
GAGGAAAGGC ACATAAGCAA GCTCCTGGCA GCAGCAAGAC TCTTCAAACC TCTCGATCCA 
GACGCAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139765.1
CDS	249..3740
Translation	MEELRKRRVSQDLDVEKPDASPTSLQLRSQIEESLGLSSTASTPDTERKLSIHKSSSEEGSVGKADWKKKNKFFWQNFRKNQKGLMRQTSKGEDVGYVASEITMSDEERIQLMMMVKEKMITIEEALARLKEYESQHRQSSTVDATDWPDGSYSTFDGSSNCNSREQSDDETEESVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGVEEQSLDNSAILDDRSALYSGVHKKQFYFDGSSEKQTEDDSDSLTTSPSSSSLDTWGASRKLVKTFSKTDSRGLIKPPKKLGTFFSYPEDEKTQKVCRSLTDGEMKKSLGSLSHGRTCSFGGFDLTNRSLHIGNNSDQMGKEGDFVYKEVIKSPSASRISLGKKVKSVKETMKKRMSKKYSSSLSEQESSPGVIPGSPQSPPPDTDSLDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRRKSRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVEHLQEYDSNSDQSGSQEKLLIEGQGLAGCSPRDSGCYESSENLENGKTRRTCLLPSKSSSEHSFKDFSRHQLSNYPTLPLTKSIETLQQGEKESRLSCAHRALKSSVKTPAVMGLKKNRRSLPVAVCRSYETLDGPQSVDVWPRSHSLDDLQGDSDTNLQDGSKKDYFARDSLDTAKKATGSALPPQSCGGSCMVDNLMPKQGKAAAGSQKGRGKELECSVMESAVGKPFPFPMKHCEAQAALVTHSATRTPLEIQSKGFHDLARADYAPVLKGGLEAEQKGTNETRMQPKNPSQPPPVPAKKCRERLTNGLYHPPMTPSGNNSSQEAPCLPVKKTSASNPTDFHGVPVHRTSEQEPSTPPSSLPPWLLEQPETKSVQQHVIKLGPASARKVSCSRGMDLEMVIENKLQSEGIDLTEEPYSDKHGRCGIPEALVQRYSEDLDQPEKDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGLITSVSDWLISIGLPMYSSPLTEAGFNTLSKVPSLSQTCLQEAGITEERHISKLLAAARLFKPLDPDAI

Target ORF information:

RefSeq Version	XM_015284279.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015284279.1

ORF Insert Sequence:

ATGGAAGAGC TGCGCAAACG GAGGGTTTCC CAGGACCTTG ATGTGGAGAA ACCTGATGCC 
AGTCCCACAT CTCTACAACT GCGATCCCAG ATTGAGGAGT CACTTGGTCT CAGCAGCACC 
GCATCAACAC CTGACACTGA AAGAAAGCTC TCCATTCATA AATCAAGTTC GGAAGAAGGC 
TCTGTAGGGA AAGCAGACTG GAAAAAGAAA AATAAGTTTT TTTGGCAAAA TTTCCGAAAG 
AACCAGAAAG GATTGATGAG GCAGACTTCA AAAGGAGAGG ATGTGGGGTA TGTGGCCAGT 
GAGATTACGA TGAGCGATGA GGAACGGATC CAGCTGATGA TGATGGTGAA AGAGAAGATG 
ATCACTATTG AGGAAGCACT TGCTAGGCTG AAGGAGTATG AGTCCCAGCA CAGGCAATCA 
AGTACTGTAG ATGCTACAGA CTGGCCTGAT GGTTCTTACT CAACATTTGA TGGGTCTTCC 
AATTGTAATT CAAGAGAACA ATCCGATGAT GAAACAGAGG AGTCGGTGAA GTTTAAGAGA 
TTGCACAAGC TGGTAAATTC TACTCGCAGA GTCAGGAAGA AACTCATAAG GGTGGAAGAA 
ATGAAAAAAC CCAGTACAGA AGGCGTAGAG GAGCAGTCGC TTGACAACTC TGCTATACTG 
GATGACAGAT CGGCACTTTA CTCTGGAGTT CACAAGAAGC AATTCTACTT TGATGGTTCC 
TCCGAGAAGC AGACAGAGGA TGACTCGGAC TCACTTACTA CTTCTCCCTC ATCTAGTAGT 
CTTGATACAT GGGGAGCTAG CCGGAAACTG GTGAAGACCT TCAGCAAAAC TGATAGCCGT 
GGCCTTATCA AGCCTCCCAA GAAACTTGGG ACATTTTTTT CTTACCCAGA AGATGAGAAG 
ACCCAGAAAG TTTGCCGCTC CTTGACAGAT GGGGAGATGA AGAAGAGCCT CGGCTCACTG 
AGCCATGGGA GAACCTGCAG CTTTGGAGGA TTTGACTTGA CAAATCGTTC CCTTCATATT 
GGAAACAATT CTGACCAAAT GGGCAAAGAA GGTGACTTTG TTTATAAAGA AGTAATCAAA 
TCACCCTCTG CCTCCCGTAT ATCTCTGGGC AAAAAGGTGA AATCTGTGAA AGAAACCATG 
AAGAAAAGGA TGTCTAAAAA ATACAGCAGC TCTCTGTCTG AGCAGGAGTC GAGCCCTGGA 
GTCATACCGG GTTCTCCGCA GTCGCCGCCG CCAGACACTG ACTCTCTGGA CAAGCCCAAG 
CTGAAAGCTG GTGGCTCAGT TGAGAGCCTG AGGAGTTCCT TAAGTGGTCA GAGCTCAATG 
AGTGGTCAGA CAGTGAGTAC AACAGATTCC TCAACCAGCA ACAGGGAGAG CGTGAAATCA 
GAAGATGGGG ATGATGAAGA ACCTCCCTAC AGAGGACCCT TCTGTGGAAG AGCCAGGGTT 
CACACTGATT TTACTCCAAG TCCTTATGAT ACAGACTCTC TGAAGCTCAA GAAAGGTGAT 
ATCATTGACA TCATCAGCAA ACCCCCCATG GGCACTTGGA TGGGCCTATT GAACAACAAA 
GTTGGCACTT TCAAATTTAT CTATGTGGAT GTGTTAAATG AAGAGGAAGA GAAGCCGAAG 
CGACCCACCA GGAGACGCCG AAAAAGCAGA CCACCCCAAC CCAAGTCAGT TGAGGATCTC 
TTGGATCGCA TCAACTTAAA GGAGCACATG CCCACTTTTC TGTTCAATGG TTATGAAGAT 
CTGGATACCT TTAAGCTTTT AGAAGAAGAG GATTTGGATG AACTGAACAT CCGAGATCCT 
GAGCACAGAG CTGTTCTCCT CACAGCCGTG GAACATCTAC AGGAGTATGA TAGTAACAGC 
GACCAGTCAG GATCTCAGGA GAAACTTCTC ATTGAAGGTC AAGGCCTAGC TGGATGCTCT 
CCTCGGGATT CTGGCTGCTA TGAAAGCAGT GAAAACCTGG AGAATGGTAA AACCCGAAGG 
ACCTGTCTTC TGCCCTCAAA ATCATCAAGT GAGCATAGCT TTAAAGACTT CAGCAGACAC 
CAGCTATCAA ATTATCCCAC ACTCCCACTG ACCAAGTCAA TAGAAACTCT ACAGCAAGGA 
GAAAAAGAAA GCCGGTTGAG CTGTGCACAT CGTGCTCTGA AGAGCTCTGT CAAGACCCCA 
GCTGTTATGG GTCTGAAGAA GAACCGTAGA AGTTTACCAG TTGCTGTCTG TCGGAGCTAT 
GAAACTCTGG ATGGCCCCCA GAGTGTGGAT GTATGGCCAA GATCTCACTC CCTGGACGAT 
CTCCAGGGAG ATTCCGATAC TAATCTACAA GATGGTAGCA AGAAAGATTA TTTTGCTCGG 
GATTCACTGG ATACAGCGAA AAAGGCAACT GGATCTGCCC TGCCACCTCA GTCGTGTGGG 
GGCAGTTGTA TGGTCGATAA CTTGATGCCT AAGCAGGGTA AAGCAGCTGC TGGTTCTCAG 
AAGGGTAGAG GTAAGGAGTT GGAGTGCTCT GTAATGGAGA GTGCAGTTGG GAAACCTTTT 
CCATTCCCCA TGAAACACTG TGAAGCTCAG GCTGCCTTGG TGACACATTC TGCAACAAGG 
ACACCTCTGG AAATACAGAG TAAGGGCTTT CATGACCTTG CACGGGCTGA CTATGCTCCA 
GTCCTCAAGG GAGGTCTAGA AGCTGAGCAA AAAGGCACAA ATGAGACAAG AATGCAACCA 
AAAAATCCCT CTCAGCCGCC ACCTGTTCCT GCCAAAAAAT GCCGAGAACG CCTTACTAAT 
GGATTATATC ATCCTCCCAT GACCCCCAGT GGGAACAACT CAAGTCAAGA AGCACCGTGT 
TTGCCAGTAA AAAAGACCAG TGCATCCAAT CCCACTGATT TTCATGGAGT ACCTGTCCAT 
AGGACATCTG AACAGGAGCC CAGTACCCCT CCTAGCTCAC TGCCACCCTG GCTGTTGGAG 
CAACCAGAGA CTAAAAGCGT TCAGCAGCAC GTGATAAAGC TAGGGCCTGC TTCAGCAAGG 
AAAGTCTCTT GTAGCAGGGG AATGGATCTG GAAATGGTAA TAGAAAACAA GTTGCAGTCT 
GAAGGTATTG ATCTTACTGA AGAACCATAC TCAGACAAGC ATGGTCGCTG TGGCATTCCT 
GAGGCTTTGG TACAGAGGTA CTCCGAAGAC TTAGACCAGC CTGAAAAGGA TGTTGCTGCA 
AACATGGACC AAATCCGGGT AAAGCAGCTG AGGAAGCAGC ATCGCATGGC AATTCCAAGT 
GGAGGGCTCA CTGAAATTTG CCGAAAACCT GTATCACCAG GACTTATCAC CTCCGTATCT 
GACTGGCTGA TTTCCATTGG CCTACCCATG TATTCCAGCC CACTCACAGA AGCAGGGTTC 
AACACACTGA GCAAAGTGCC TTCTCTGTCA CAAACTTGCC TTCAGGAAGC TGGCATCACA 
GAGGAAAGGC ACATAAGCAA GCTCCTGGCA GCAGCAAGAC TCTTCAAACC TCTCGATCCA 
GACGCAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH1 ( XM_015284279.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139765.1 Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OGa33755
Clone ID Related Accession (Same CDS sequence)	XM_015284279.1 , XM_015284279.2
Accession Version	XM_015284279.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	SAM and SH3 domain-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284279.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC TGCGCAAACG GAGGGTTTCC CAGGACCTTG ATGTGGAGAA ACCTGATGCC 
AGTCCCACAT CTCTACAACT GCGATCCCAG ATTGAGGAGT CACTTGGTCT CAGCAGCACC 
GCATCAACAC CTGACACTGA AAGAAAGCTC TCCATTCATA AATCAAGTTC GGAAGAAGGC 
TCTGTAGGGA AAGCAGACTG GAAAAAGAAA AATAAGTTTT TTTGGCAAAA TTTCCGAAAG 
AACCAGAAAG GATTGATGAG GCAGACTTCA AAAGGAGAGG ATGTGGGGTA TGTGGCCAGT 
GAGATTACGA TGAGCGATGA GGAACGGATC CAGCTGATGA TGATGGTGAA AGAGAAGATG 
ATCACTATTG AGGAAGCACT TGCTAGGCTG AAGGAGTATG AGTCCCAGCA CAGGCAATCA 
AGTACTGTAG ATGCTACAGA CTGGCCTGAT GGTTCTTACT CAACATTTGA TGGGTCTTCC 
AATTGTAATT CAAGAGAACA ATCCGATGAT GAAACAGAGG AGTCGGTGAA GTTTAAGAGA 
TTGCACAAGC TGGTAAATTC TACTCGCAGA GTCAGGAAGA AACTCATAAG GGTGGAAGAA 
ATGAAAAAAC CCAGTACAGA AGGCGTAGAG GAGCAGTCGC TTGACAACTC TGCTATACTG 
GATGACAGAT CGGCACTTTA CTCTGGAGTT CACAAGAAGC AATTCTACTT TGATGGTTCC 
TCCGAGAAGC AGACAGAGGA TGACTCGGAC TCACTTACTA CTTCTCCCTC ATCTAGTAGT 
CTTGATACAT GGGGAGCTAG CCGGAAACTG GTGAAGACCT TCAGCAAAAC TGATAGCCGT 
GGCCTTATCA AGCCTCCCAA GAAACTTGGG ACATTTTTTT CTTACCCAGA AGATGAGAAG 
ACCCAGAAAG TTTGCCGCTC CTTGACAGAT GGGGAGATGA AGAAGAGCCT CGGCTCACTG 
AGCCATGGGA GAACCTGCAG CTTTGGAGGA TTTGACTTGA CAAATCGTTC CCTTCATATT 
GGAAACAATT CTGACCAAAT GGGCAAAGAA GGTGACTTTG TTTATAAAGA AGTAATCAAA 
TCACCCTCTG CCTCCCGTAT ATCTCTGGGC AAAAAGGTGA AATCTGTGAA AGAAACCATG 
AAGAAAAGGA TGTCTAAAAA ATACAGCAGC TCTCTGTCTG AGCAGGAGTC GAGCCCTGGA 
GTCATACCGG GTTCTCCGCA GTCGCCGCCG CCAGACACTG ACTCTCTGGA CAAGCCCAAG 
CTGAAAGCTG GTGGCTCAGT TGAGAGCCTG AGGAGTTCCT TAAGTGGTCA GAGCTCAATG 
AGTGGTCAGA CAGTGAGTAC AACAGATTCC TCAACCAGCA ACAGGGAGAG CGTGAAATCA 
GAAGATGGGG ATGATGAAGA ACCTCCCTAC AGAGGACCCT TCTGTGGAAG AGCCAGGGTT 
CACACTGATT TTACTCCAAG TCCTTATGAT ACAGACTCTC TGAAGCTCAA GAAAGGTGAT 
ATCATTGACA TCATCAGCAA ACCCCCCATG GGCACTTGGA TGGGCCTATT GAACAACAAA 
GTTGGCACTT TCAAATTTAT CTATGTGGAT GTGTTAAATG AAGAGGAAGA GAAGCCGAAG 
CGACCCACCA GGAGACGCCG AAAAAGCAGA CCACCCCAAC CCAAGTCAGT TGAGGATCTC 
TTGGATCGCA TCAACTTAAA GGAGCACATG CCCACTTTTC TGTTCAATGG TTATGAAGAT 
CTGGATACCT TTAAGCTTTT AGAAGAAGAG GATTTGGATG AACTGAACAT CCGAGATCCT 
GAGCACAGAG CTGTTCTCCT CACAGCCGTG GAACATCTAC AGGAGTATGA TAGTAACAGC 
GACCAGTCAG GATCTCAGGA GAAACTTCTC ATTGAAGGTC AAGGCCTAGC TGGATGCTCT 
CCTCGGGATT CTGGCTGCTA TGAAAGCAGT GAAAACCTGG AGAATGGTAA AACCCGAAGG 
ACCTGTCTTC TGCCCTCAAA ATCATCAAGT GAGCATAGCT TTAAAGACTT CAGCAGACAC 
CAGCTATCAA ATTATCCCAC ACTCCCACTG ACCAAGTCAA TAGAAACTCT ACAGCAAGGA 
GAAAAAGAAA GCCGGTTGAG CTGTGCACAT CGTGCTCTGA AGAGCTCTGT CAAGACCCCA 
GCTGTTATGG GTCTGAAGAA GAACCGTAGA AGTTTACCAG TTGCTGTCTG TCGGAGCTAT 
GAAACTCTGG ATGGCCCCCA GAGTGTGGAT GTATGGCCAA GATCTCACTC CCTGGACGAT 
CTCCAGGGAG ATTCCGATAC TAATCTACAA GATGGTAGCA AGAAAGATTA TTTTGCTCGG 
GATTCACTGG ATACAGCGAA AAAGGCAACT GGATCTGCCC TGCCACCTCA GTCGTGTGGG 
GGCAGTTGTA TGGTCGATAA CTTGATGCCT AAGCAGGGTA AAGCAGCTGC TGGTTCTCAG 
AAGGGTAGAG GTAAGGAGTT GGAGTGCTCT GTAATGGAGA GTGCAGTTGG GAAACCTTTT 
CCATTCCCCA TGAAACACTG TGAAGCTCAG GCTGCCTTGG TGACACATTC TGCAACAAGG 
ACACCTCTGG AAATACAGAG TAAGGGCTTT CATGACCTTG CACGGGCTGA CTATGCTCCA 
GTCCTCAAGG GAGGTCTAGA AGCTGAGCAA AAAGGCACAA ATGAGACAAG AATGCAACCA 
AAAAATCCCT CTCAGCCGCC ACCTGTTCCT GCCAAAAAAT GCCGAGAACG CCTTACTAAT 
GGATTATATC ATCCTCCCAT GACCCCCAGT GGGAACAACT CAAGTCAAGA AGCACCGTGT 
TTGCCAGTAA AAAAGACCAG TGCATCCAAT CCCACTGATT TTCATGGAGT ACCTGTCCAT 
AGGACATCTG AACAGGAGCC CAGTACCCCT CCTAGCTCAC TGCCACCCTG GCTGTTGGAG 
CAACCAGAGA CTAAAAGCGT TCAGCAGCAC GTGATAAAGC TAGGGCCTGC TTCAGCAAGG 
AAAGTCTCTT GTAGCAGGGG AATGGATCTG GAAATGGTAA TAGAAAACAA GTTGCAGTCT 
GAAGGTATTG ATCTTACTGA AGAACCATAC TCAGACAAGC ATGGTCGCTG TGGCATTCCT 
GAGGCTTTGG TACAGAGGTA CTCCGAAGAC TTAGACCAGC CTGAAAAGGA TGTTGCTGCA 
AACATGGACC AAATCCGGGT AAAGCAGCTG AGGAAGCAGC ATCGCATGGC AATTCCAAGT 
GGAGGGCTCA CTGAAATTTG CCGAAAACCT GTATCACCAG GACTTATCAC CTCCGTATCT 
GACTGGCTGA TTTCCATTGG CCTACCCATG TATTCCAGCC CACTCACAGA AGCAGGGTTC 
AACACACTGA GCAAAGTGCC TTCTCTGTCA CAAACTTGCC TTCAGGAAGC TGGCATCACA 
GAGGAAAGGC ACATAAGCAA GCTCCTGGCA GCAGCAAGAC TCTTCAAACC TCTCGATCCA 
GACGCAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139765.1
CDS	249..3740
Translation	MEELRKRRVSQDLDVEKPDASPTSLQLRSQIEESLGLSSTASTPDTERKLSIHKSSSEEGSVGKADWKKKNKFFWQNFRKNQKGLMRQTSKGEDVGYVASEITMSDEERIQLMMMVKEKMITIEEALARLKEYESQHRQSSTVDATDWPDGSYSTFDGSSNCNSREQSDDETEESVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGVEEQSLDNSAILDDRSALYSGVHKKQFYFDGSSEKQTEDDSDSLTTSPSSSSLDTWGASRKLVKTFSKTDSRGLIKPPKKLGTFFSYPEDEKTQKVCRSLTDGEMKKSLGSLSHGRTCSFGGFDLTNRSLHIGNNSDQMGKEGDFVYKEVIKSPSASRISLGKKVKSVKETMKKRMSKKYSSSLSEQESSPGVIPGSPQSPPPDTDSLDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRRKSRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVEHLQEYDSNSDQSGSQEKLLIEGQGLAGCSPRDSGCYESSENLENGKTRRTCLLPSKSSSEHSFKDFSRHQLSNYPTLPLTKSIETLQQGEKESRLSCAHRALKSSVKTPAVMGLKKNRRSLPVAVCRSYETLDGPQSVDVWPRSHSLDDLQGDSDTNLQDGSKKDYFARDSLDTAKKATGSALPPQSCGGSCMVDNLMPKQGKAAAGSQKGRGKELECSVMESAVGKPFPFPMKHCEAQAALVTHSATRTPLEIQSKGFHDLARADYAPVLKGGLEAEQKGTNETRMQPKNPSQPPPVPAKKCRERLTNGLYHPPMTPSGNNSSQEAPCLPVKKTSASNPTDFHGVPVHRTSEQEPSTPPSSLPPWLLEQPETKSVQQHVIKLGPASARKVSCSRGMDLEMVIENKLQSEGIDLTEEPYSDKHGRCGIPEALVQRYSEDLDQPEKDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGLITSVSDWLISIGLPMYSSPLTEAGFNTLSKVPSLSQTCLQEAGITEERHISKLLAAARLFKPLDPDAI

Target ORF information:

RefSeq Version	XM_015284279.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015284279.2

ORF Insert Sequence:

ATGGAAGAGC TGCGCAAACG GAGGGTTTCC CAGGACCTTG ATGTGGAGAA ACCTGATGCC 
AGTCCCACAT CTCTACAACT GCGATCCCAG ATTGAGGAGT CACTTGGTCT CAGCAGCACC 
GCATCAACAC CTGACACTGA AAGAAAGCTC TCCATTCATA AATCAAGTTC GGAAGAAGGC 
TCTGTAGGGA AAGCAGACTG GAAAAAGAAA AATAAGTTTT TTTGGCAAAA TTTCCGAAAG 
AACCAGAAAG GATTGATGAG GCAGACTTCA AAAGGAGAGG ATGTGGGGTA TGTGGCCAGT 
GAGATTACGA TGAGCGATGA GGAACGGATC CAGCTGATGA TGATGGTGAA AGAGAAGATG 
ATCACTATTG AGGAAGCACT TGCTAGGCTG AAGGAGTATG AGTCCCAGCA CAGGCAATCA 
AGTACTGTAG ATGCTACAGA CTGGCCTGAT GGTTCTTACT CAACATTTGA TGGGTCTTCC 
AATTGTAATT CAAGAGAACA ATCCGATGAT GAAACAGAGG AGTCGGTGAA GTTTAAGAGA 
TTGCACAAGC TGGTAAATTC TACTCGCAGA GTCAGGAAGA AACTCATAAG GGTGGAAGAA 
ATGAAAAAAC CCAGTACAGA AGGCGTAGAG GAGCAGTCGC TTGACAACTC TGCTATACTG 
GATGACAGAT CGGCACTTTA CTCTGGAGTT CACAAGAAGC AATTCTACTT TGATGGTTCC 
TCCGAGAAGC AGACAGAGGA TGACTCGGAC TCACTTACTA CTTCTCCCTC ATCTAGTAGT 
CTTGATACAT GGGGAGCTAG CCGGAAACTG GTGAAGACCT TCAGCAAAAC TGATAGCCGT 
GGCCTTATCA AGCCTCCCAA GAAACTTGGG ACATTTTTTT CTTACCCAGA AGATGAGAAG 
ACCCAGAAAG TTTGCCGCTC CTTGACAGAT GGGGAGATGA AGAAGAGCCT CGGCTCACTG 
AGCCATGGGA GAACCTGCAG CTTTGGAGGA TTTGACTTGA CAAATCGTTC CCTTCATATT 
GGAAACAATT CTGACCAAAT GGGCAAAGAA GGTGACTTTG TTTATAAAGA AGTAATCAAA 
TCACCCTCTG CCTCCCGTAT ATCTCTGGGC AAAAAGGTGA AATCTGTGAA AGAAACCATG 
AAGAAAAGGA TGTCTAAAAA ATACAGCAGC TCTCTGTCTG AGCAGGAGTC GAGCCCTGGA 
GTCATACCGG GTTCTCCGCA GTCGCCGCCG CCAGACACTG ACTCTCTGGA CAAGCCCAAG 
CTGAAAGCTG GTGGCTCAGT TGAGAGCCTG AGGAGTTCCT TAAGTGGTCA GAGCTCAATG 
AGTGGTCAGA CAGTGAGTAC AACAGATTCC TCAACCAGCA ACAGGGAGAG CGTGAAATCA 
GAAGATGGGG ATGATGAAGA ACCTCCCTAC AGAGGACCCT TCTGTGGAAG AGCCAGGGTT 
CACACTGATT TTACTCCAAG TCCTTATGAT ACAGACTCTC TGAAGCTCAA GAAAGGTGAT 
ATCATTGACA TCATCAGCAA ACCCCCCATG GGCACTTGGA TGGGCCTATT GAACAACAAA 
GTTGGCACTT TCAAATTTAT CTATGTGGAT GTGTTAAATG AAGAGGAAGA GAAGCCGAAG 
CGACCCACCA GGAGACGCCG AAAAAGCAGA CCACCCCAAC CCAAGTCAGT TGAGGATCTC 
TTGGATCGCA TCAACTTAAA GGAGCACATG CCCACTTTTC TGTTCAATGG TTATGAAGAT 
CTGGATACCT TTAAGCTTTT AGAAGAAGAG GATTTGGATG AACTGAACAT CCGAGATCCT 
GAGCACAGAG CTGTTCTCCT CACAGCCGTG GAACATCTAC AGGAGTATGA TAGTAACAGC 
GACCAGTCAG GATCTCAGGA GAAACTTCTC ATTGAAGGTC AAGGCCTAGC TGGATGCTCT 
CCTCGGGATT CTGGCTGCTA TGAAAGCAGT GAAAACCTGG AGAATGGTAA AACCCGAAGG 
ACCTGTCTTC TGCCCTCAAA ATCATCAAGT GAGCATAGCT TTAAAGACTT CAGCAGACAC 
CAGCTATCAA ATTATCCCAC ACTCCCACTG ACCAAGTCAA TAGAAACTCT ACAGCAAGGA 
GAAAAAGAAA GCCGGTTGAG CTGTGCACAT CGTGCTCTGA AGAGCTCTGT CAAGACCCCA 
GCTGTTATGG GTCTGAAGAA GAACCGTAGA AGTTTACCAG TTGCTGTCTG TCGGAGCTAT 
GAAACTCTGG ATGGCCCCCA GAGTGTGGAT GTATGGCCAA GATCTCACTC CCTGGACGAT 
CTCCAGGGAG ATTCCGATAC TAATCTACAA GATGGTAGCA AGAAAGATTA TTTTGCTCGG 
GATTCACTGG ATACAGCGAA AAAGGCAACT GGATCTGCCC TGCCACCTCA GTCGTGTGGG 
GGCAGTTGTA TGGTCGATAA CTTGATGCCT AAGCAGGGTA AAGCAGCTGC TGGTTCTCAG 
AAGGGTAGAG GTAAGGAGTT GGAGTGCTCT GTAATGGAGA GTGCAGTTGG GAAACCTTTT 
CCATTCCCCA TGAAACACTG TGAAGCTCAG GCTGCCTTGG TGACACATTC TGCAACAAGG 
ACACCTCTGG AAATACAGAG TAAGGGCTTT CATGACCTTG CACGGGCTGA CTATGCTCCA 
GTCCTCAAGG GAGGTCTAGA AGCTGAGCAA AAAGGCACAA ATGAGACAAG AATGCAACCA 
AAAAATCCCT CTCAGCCGCC ACCTGTTCCT GCCAAAAAAT GCCGAGAACG CCTTACTAAT 
GGATTATATC ATCCTCCCAT GACCCCCAGT GGGAACAACT CAAGTCAAGA AGCACCGTGT 
TTGCCAGTAA AAAAGACCAG TGCATCCAAT CCCACTGATT TTCATGGAGT ACCTGTCCAT 
AGGACATCTG AACAGGAGCC CAGTACCCCT CCTAGCTCAC TGCCACCCTG GCTGTTGGAG 
CAACCAGAGA CTAAAAGCGT TCAGCAGCAC GTGATAAAGC TAGGGCCTGC TTCAGCAAGG 
AAAGTCTCTT GTAGCAGGGG AATGGATCTG GAAATGGTAA TAGAAAACAA GTTGCAGTCT 
GAAGGTATTG ATCTTACTGA AGAACCATAC TCAGACAAGC ATGGTCGCTG TGGCATTCCT 
GAGGCTTTGG TACAGAGGTA CTCCGAAGAC TTAGACCAGC CTGAAAAGGA TGTTGCTGCA 
AACATGGACC AAATCCGGGT AAAGCAGCTG AGGAAGCAGC ATCGCATGGC AATTCCAAGT 
GGAGGGCTCA CTGAAATTTG CCGAAAACCT GTATCACCAG GACTTATCAC CTCCGTATCT 
GACTGGCTGA TTTCCATTGG CCTACCCATG TATTCCAGCC CACTCACAGA AGCAGGGTTC 
AACACACTGA GCAAAGTGCC TTCTCTGTCA CAAACTTGCC TTCAGGAAGC TGGCATCACA 
GAGGAAAGGC ACATAAGCAA GCTCCTGGCA GCAGCAAGAC TCTTCAAACC TCTCGATCCA 
GACGCAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH1 ( XM_004935599.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004935656.1 Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OGa06284
Clone ID Related Accession (Same CDS sequence)	XM_004935599.2
Accession Version	XM_004935599.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	SAM and SH3 domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004935599.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAAT TTTCACGTGA AACTGAGTTT TGGAATAAGG TGATGGATGG GTCTCTGGGA 
AACATTGATG ACCTCGCACA GCAGTATGCA GATTATTACA ACACCTGCTT CACGGATGTG 
TGTGAGAGGA TGGAAGAGCT GCGCAAACGG AGGGTTTCCC AGGACCTTGA TGTGGAGAAA 
CCTGATGCCA GTCCCACATC TCTACAACTG CGATCCCAGA TTGAGGAGTC ACTTGGTCTC 
AGCAGCACCG CATCAACACC TGACACTGAA AGAAAGCTCT CCATTCATAA ATCAAGTTCG 
GAAGAAGGCT CTGTAGGGAA AGCAGACTGG AAAAAGAAAA ATAAGTTTTT TTGGCAAAAT 
TTCCGAAAGA ACCAGAAAGG ATTGATGAGG CAGACTTCAA AAGGAGAGGA TGTGGGGTAT 
GTGGCCAGTG AGATTACGAT GAGCGATGAG GAACGGATCC AGCTGATGAT GATGGTGAAA 
GAGAAGATGA TCACTATTGA GGAAGCACTT GCTAGGCTGA AGGAGTATGA GTCCCAGCAC 
AGGCAATCAA GTACTGTAGA TGCTACAGAC TGGCCTGATG GTTCTTACTC AACATTTGAT 
GGGTCTTCCA ATTGTAATTC AAGAGAACAA TCCGATGATG AAACAGAGGA GTCGGTGAAG 
TTTAAGAGAT TGCACAAGCT GGTAAATTCT ACTCGCAGAG TCAGGAAGAA ACTCATAAGG 
GTGGAAGAAA TGAAAAAACC CAGTACAGAA GGCGTAGAGG AGCAGTCGCT TGACAACTCT 
GCTATACTGG ATGACAGATC GGCACTTTAC TCTGGAGTTC ACAAGAAGCA ATTCTACTTT 
GATGGTTCCT CCGAGAAGCA GACAGAGGAT GACTCGGACT CACTTACTAC TTCTCCCTCA 
TCTAGTAGTC TTGATACATG GGGAGCTAGC CGGAAACTGG TGAAGACCTT CAGCAAAACT 
GATAGCCGTG GCCTTATCAA GCCTCCCAAG AAACTTGGGA CATTTTTTTC TTACCCAGAA 
GATGAGAAGA CCCAGAAAGT TTGCCGCTCC TTGACAGATG GGGAGATGAA GAAGAGCCTC 
GGCTCACTGA GCCATGGGAG AACCTGCAGC TTTGGAGGAT TTGACTTGAC AAATCGTTCC 
CTTCATATTG GAAACAATTC TGACCAAATG GGCAAAGAAG GTGACTTTGT TTATAAAGAA 
GTAATCAAAT CACCCTCTGC CTCCCGTATA TCTCTGGGCA AAAAGGTGAA ATCTGTGAAA 
GAAACCATGA AGAAAAGGAT GTCTAAAAAA TACAGCAGCT CTCTGTCTGA GCAGGAGTCG 
AGCCCTGGAG TCATACCGGG TTCTCCGCAG TCGCCGCCGC CAGACACTGA CTCTCTGGAC 
AAGCCCAAGC TGAAAGCTGG TGGCTCAGTT GAGAGCCTGA GGAGTTCCTT AAGTGGTCAG 
AGCTCAATGA GTGGTCAGAC AGTGAGTACA ACAGATTCCT CAACCAGCAA CAGGGAGAGC 
GTGAAATCAG AAGATGGGGA TGATGAAGAA CCTCCCTACA GAGGACCCTT CTGTGGAAGA 
GCCAGGGTTC ACACTGATTT TACTCCAAGT CCTTATGATA CAGACTCTCT GAAGCTCAAG 
AAAGGTGATA TCATTGACAT CATCAGCAAA CCCCCCATGG GCACTTGGAT GGGCCTATTG 
AACAACAAAG TTGGCACTTT CAAATTTATC TATGTGGATG TGTTAAATGA AGAGGAAGAG 
AAGCCGAAGC GACCCACCAG GAGACGCCGA AAAAGCAGAC CACCCCAACC CAAGTCAGTT 
GAGGATCTCT TGGATCGCAT CAACTTAAAG GAGCACATGC CCACTTTTCT GTTCAATGGT 
TATGAAGATC TGGATACCTT TAAGCTTTTA GAAGAAGAGG ATTTGGATGA ACTGAACATC 
CGAGATCCTG AGCACAGAGC TGTTCTCCTC ACAGCCGTGG AACATCTACA GGAGTATGAT 
AGTAACAGCG ACCAGTCAGG ATCTCAGGAG AAACTTCTCA TTGAAGGTCA AGGCCTAGCT 
GGATGCTCTC CTCGGGATTC TGGCTGCTAT GAAAGCAGTG AAAACCTGGA GAATGGTAAA 
ACCCGAAGGA CCTGTCTTCT GCCCTCAAAA TCATCAAGTG AGCATAGCTT TAAAGACTTC 
AGCAGACACC AGCTATCAAA TTATCCCACA CTCCCACTGA CCAAGTCAAT AGAAACTCTA 
CAGCAAGGAG AAAAAGAAAG CCGGTTGAGC TGTGCACATC GTGCTCTGAA GAGCTCTGTC 
AAGACCCCAG CTGTTATGGG TCTGAAGAAG AACCGTAGAA GTTTACCAGT TGCTGTCTGT 
CGGAGCTATG AAACTCTGGA TGGCCCCCAG AGTGTGGATG TATGGCCAAG ATCTCACTCC 
CTGGACGATC TCCAGGGAGA TTCCGATACT AATCTACAAG ATGGTAGCAA GAAAGATTAT 
TTTGCTCGGG ATTCACTGGA TACAGCGAAA AAGGCAACTG GATCTGCCCT GCCACCTCAG 
TCGTGTGGGG GCAGTTGTAT GGTCGATAAC TTGATGCCTA AGCAGGGTAA AGCAGCTGCT 
GGTTCTCAGA AGGGTAGAGG TAAGGAGTTG GAGTGCTCTG TAATGGAGAG TGCAGTTGGG 
AAACCTTTTC CATTCCCCAT GAAACACTGT GAAGCTCAGG CTGCCTTGGT GACACATTCT 
GCAACAAGGA CACCTCTGGA AATACAGAGT AAGGGCTTTC ATGACCTTGC ACGGGCTGAC 
TATGCTCCAG TCCTCAAGGG AGGTCTAGAA GCTGAGCAAA AAGGCACAAA TGAGACAAGA 
ATGCAACCAA AAAATCCCTC TCAGCCGCCA CCTGTTCCTG CCAAAAAATG CCGAGAACGC 
CTTACTAATG GATTATATCA TCCTCCCATG ACCCCCAGTG GGAACAACTC AAGTCAAGAA 
GCACCGTGTT TGCCAGTAAA AAAGACCAGT GCATCCAATC CCACTGATTT TCATGGAGTA 
CCTGTCCATA GGACATCTGA ACAGGAGCCC AGTACCCCTC CTAGCTCACT GCCACCCTGG 
CTGTTGGAGC AACCAGAGAC TAAAAGCGTT CAGCAGCACG TGATAAAGCT AGGGCCTGCT 
TCAGCAAGGA AAGTCTCTTG TAGCAGGGGA ATGGATCTGG AAATGGTAAT AGAAAACAAG 
TTGCAGTCTG AAGGTATTGA TCTTACTGAA GAACCATACT CAGACAAGCA TGGTCGCTGT 
GGCATTCCTG AGGCTTTGGT ACAGAGGTAC TCCGAAGACT TAGACCAGCC TGAAAAGGAT 
GTTGCTGCAA ACATGGACCA AATCCGGGTA AAGCAGCTGA GGAAGCAGCA TCGCATGGCA 
ATTCCAAGTG GAGGGCTCAC TGAAATTTGC CGAAAACCTG TATCACCAGG ACTTATCACC 
TCCGTATCTG ACTGGCTGAT TTCCATTGGC CTACCCATGT ATTCCAGCCC ACTCACAGAA 
GCAGGGTTCA ACACACTGAG CAAAGTGCCT TCTCTGTCAC AAACTTGCCT TCAGGAAGCT 
GGCATCACAG AGGAAAGGCA CATAAGCAAG CTCCTGGCAG CAGCAAGACT CTTCAAACCT 
CTCGATCCAG ACGCAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004935656.1
CDS	117..3737
Translation	MDQFSRETEFWNKVMDGSLGNIDDLAQQYADYYNTCFTDVCERMEELRKRRVSQDLDVEKPDASPTSLQLRSQIEESLGLSSTASTPDTERKLSIHKSSSEEGSVGKADWKKKNKFFWQNFRKNQKGLMRQTSKGEDVGYVASEITMSDEERIQLMMMVKEKMITIEEALARLKEYESQHRQSSTVDATDWPDGSYSTFDGSSNCNSREQSDDETEESVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGVEEQSLDNSAILDDRSALYSGVHKKQFYFDGSSEKQTEDDSDSLTTSPSSSSLDTWGASRKLVKTFSKTDSRGLIKPPKKLGTFFSYPEDEKTQKVCRSLTDGEMKKSLGSLSHGRTCSFGGFDLTNRSLHIGNNSDQMGKEGDFVYKEVIKSPSASRISLGKKVKSVKETMKKRMSKKYSSSLSEQESSPGVIPGSPQSPPPDTDSLDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRRKSRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVEHLQEYDSNSDQSGSQEKLLIEGQGLAGCSPRDSGCYESSENLENGKTRRTCLLPSKSSSEHSFKDFSRHQLSNYPTLPLTKSIETLQQGEKESRLSCAHRALKSSVKTPAVMGLKKNRRSLPVAVCRSYETLDGPQSVDVWPRSHSLDDLQGDSDTNLQDGSKKDYFARDSLDTAKKATGSALPPQSCGGSCMVDNLMPKQGKAAAGSQKGRGKELECSVMESAVGKPFPFPMKHCEAQAALVTHSATRTPLEIQSKGFHDLARADYAPVLKGGLEAEQKGTNETRMQPKNPSQPPPVPAKKCRERLTNGLYHPPMTPSGNNSSQEAPCLPVKKTSASNPTDFHGVPVHRTSEQEPSTPPSSLPPWLLEQPETKSVQQHVIKLGPASARKVSCSRGMDLEMVIENKLQSEGIDLTEEPYSDKHGRCGIPEALVQRYSEDLDQPEKDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGLITSVSDWLISIGLPMYSSPLTEAGFNTLSKVPSLSQTCLQEAGITEERHISKLLAAARLFKPLDPDAI

Target ORF information:

RefSeq Version	XM_004935599.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004935599.2

ORF Insert Sequence:

ATGGATCAAT TTTCACGTGA AACTGAGTTT TGGAATAAGG TGATGGATGG GTCTCTGGGA 
AACATTGATG ACCTCGCACA GCAGTATGCA GATTATTACA ACACCTGCTT CACGGATGTG 
TGTGAGAGGA TGGAAGAGCT GCGCAAACGG AGGGTTTCCC AGGACCTTGA TGTGGAGAAA 
CCTGATGCCA GTCCCACATC TCTACAACTG CGATCCCAGA TTGAGGAGTC ACTTGGTCTC 
AGCAGCACCG CATCAACACC TGACACTGAA AGAAAGCTCT CCATTCATAA ATCAAGTTCG 
GAAGAAGGCT CTGTAGGGAA AGCAGACTGG AAAAAGAAAA ATAAGTTTTT TTGGCAAAAT 
TTCCGAAAGA ACCAGAAAGG ATTGATGAGG CAGACTTCAA AAGGAGAGGA TGTGGGGTAT 
GTGGCCAGTG AGATTACGAT GAGCGATGAG GAACGGATCC AGCTGATGAT GATGGTGAAA 
GAGAAGATGA TCACTATTGA GGAAGCACTT GCTAGGCTGA AGGAGTATGA GTCCCAGCAC 
AGGCAATCAA GTACTGTAGA TGCTACAGAC TGGCCTGATG GTTCTTACTC AACATTTGAT 
GGGTCTTCCA ATTGTAATTC AAGAGAACAA TCCGATGATG AAACAGAGGA GTCGGTGAAG 
TTTAAGAGAT TGCACAAGCT GGTAAATTCT ACTCGCAGAG TCAGGAAGAA ACTCATAAGG 
GTGGAAGAAA TGAAAAAACC CAGTACAGAA GGCGTAGAGG AGCAGTCGCT TGACAACTCT 
GCTATACTGG ATGACAGATC GGCACTTTAC TCTGGAGTTC ACAAGAAGCA ATTCTACTTT 
GATGGTTCCT CCGAGAAGCA GACAGAGGAT GACTCGGACT CACTTACTAC TTCTCCCTCA 
TCTAGTAGTC TTGATACATG GGGAGCTAGC CGGAAACTGG TGAAGACCTT CAGCAAAACT 
GATAGCCGTG GCCTTATCAA GCCTCCCAAG AAACTTGGGA CATTTTTTTC TTACCCAGAA 
GATGAGAAGA CCCAGAAAGT TTGCCGCTCC TTGACAGATG GGGAGATGAA GAAGAGCCTC 
GGCTCACTGA GCCATGGGAG AACCTGCAGC TTTGGAGGAT TTGACTTGAC AAATCGTTCC 
CTTCATATTG GAAACAATTC TGACCAAATG GGCAAAGAAG GTGACTTTGT TTATAAAGAA 
GTAATCAAAT CACCCTCTGC CTCCCGTATA TCTCTGGGCA AAAAGGTGAA ATCTGTGAAA 
GAAACCATGA AGAAAAGGAT GTCTAAAAAA TACAGCAGCT CTCTGTCTGA GCAGGAGTCG 
AGCCCTGGAG TCATACCGGG TTCTCCGCAG TCGCCGCCGC CAGACACTGA CTCTCTGGAC 
AAGCCCAAGC TGAAAGCTGG TGGCTCAGTT GAGAGCCTGA GGAGTTCCTT AAGTGGTCAG 
AGCTCAATGA GTGGTCAGAC AGTGAGTACA ACAGATTCCT CAACCAGCAA CAGGGAGAGC 
GTGAAATCAG AAGATGGGGA TGATGAAGAA CCTCCCTACA GAGGACCCTT CTGTGGAAGA 
GCCAGGGTTC ACACTGATTT TACTCCAAGT CCTTATGATA CAGACTCTCT GAAGCTCAAG 
AAAGGTGATA TCATTGACAT CATCAGCAAA CCCCCCATGG GCACTTGGAT GGGCCTATTG 
AACAACAAAG TTGGCACTTT CAAATTTATC TATGTGGATG TGTTAAATGA AGAGGAAGAG 
AAGCCGAAGC GACCCACCAG GAGACGCCGA AAAAGCAGAC CACCCCAACC CAAGTCAGTT 
GAGGATCTCT TGGATCGCAT CAACTTAAAG GAGCACATGC CCACTTTTCT GTTCAATGGT 
TATGAAGATC TGGATACCTT TAAGCTTTTA GAAGAAGAGG ATTTGGATGA ACTGAACATC 
CGAGATCCTG AGCACAGAGC TGTTCTCCTC ACAGCCGTGG AACATCTACA GGAGTATGAT 
AGTAACAGCG ACCAGTCAGG ATCTCAGGAG AAACTTCTCA TTGAAGGTCA AGGCCTAGCT 
GGATGCTCTC CTCGGGATTC TGGCTGCTAT GAAAGCAGTG AAAACCTGGA GAATGGTAAA 
ACCCGAAGGA CCTGTCTTCT GCCCTCAAAA TCATCAAGTG AGCATAGCTT TAAAGACTTC 
AGCAGACACC AGCTATCAAA TTATCCCACA CTCCCACTGA CCAAGTCAAT AGAAACTCTA 
CAGCAAGGAG AAAAAGAAAG CCGGTTGAGC TGTGCACATC GTGCTCTGAA GAGCTCTGTC 
AAGACCCCAG CTGTTATGGG TCTGAAGAAG AACCGTAGAA GTTTACCAGT TGCTGTCTGT 
CGGAGCTATG AAACTCTGGA TGGCCCCCAG AGTGTGGATG TATGGCCAAG ATCTCACTCC 
CTGGACGATC TCCAGGGAGA TTCCGATACT AATCTACAAG ATGGTAGCAA GAAAGATTAT 
TTTGCTCGGG ATTCACTGGA TACAGCGAAA AAGGCAACTG GATCTGCCCT GCCACCTCAG 
TCGTGTGGGG GCAGTTGTAT GGTCGATAAC TTGATGCCTA AGCAGGGTAA AGCAGCTGCT 
GGTTCTCAGA AGGGTAGAGG TAAGGAGTTG GAGTGCTCTG TAATGGAGAG TGCAGTTGGG 
AAACCTTTTC CATTCCCCAT GAAACACTGT GAAGCTCAGG CTGCCTTGGT GACACATTCT 
GCAACAAGGA CACCTCTGGA AATACAGAGT AAGGGCTTTC ATGACCTTGC ACGGGCTGAC 
TATGCTCCAG TCCTCAAGGG AGGTCTAGAA GCTGAGCAAA AAGGCACAAA TGAGACAAGA 
ATGCAACCAA AAAATCCCTC TCAGCCGCCA CCTGTTCCTG CCAAAAAATG CCGAGAACGC 
CTTACTAATG GATTATATCA TCCTCCCATG ACCCCCAGTG GGAACAACTC AAGTCAAGAA 
GCACCGTGTT TGCCAGTAAA AAAGACCAGT GCATCCAATC CCACTGATTT TCATGGAGTA 
CCTGTCCATA GGACATCTGA ACAGGAGCCC AGTACCCCTC CTAGCTCACT GCCACCCTGG 
CTGTTGGAGC AACCAGAGAC TAAAAGCGTT CAGCAGCACG TGATAAAGCT AGGGCCTGCT 
TCAGCAAGGA AAGTCTCTTG TAGCAGGGGA ATGGATCTGG AAATGGTAAT AGAAAACAAG 
TTGCAGTCTG AAGGTATTGA TCTTACTGAA GAACCATACT CAGACAAGCA TGGTCGCTGT 
GGCATTCCTG AGGCTTTGGT ACAGAGGTAC TCCGAAGACT TAGACCAGCC TGAAAAGGAT 
GTTGCTGCAA ACATGGACCA AATCCGGGTA AAGCAGCTGA GGAAGCAGCA TCGCATGGCA 
ATTCCAAGTG GAGGGCTCAC TGAAATTTGC CGAAAACCTG TATCACCAGG ACTTATCACC 
TCCGTATCTG ACTGGCTGAT TTCCATTGGC CTACCCATGT ATTCCAGCCC ACTCACAGAA 
GCAGGGTTCA ACACACTGAG CAAAGTGCCT TCTCTGTCAC AAACTTGCCT TCAGGAAGCT 
GGCATCACAG AGGAAAGGCA CATAAGCAAG CTCCTGGCAG CAGCAAGACT CTTCAAACCT 
CTCGATCCAG ACGCAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH1 ( XM_004935596.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004935653.1 Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X1, mRNA.

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CloneID	OGa06281
Clone ID Related Accession (Same CDS sequence)	XM_004935596.2 , XM_025149372.1
Accession Version	XM_004935596.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	SAM and SH3 domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004935596.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACCA ACATAGTGTA CGAGTGGCTG AAAACCCTGC AGCTCCCCCA GTACGCCGAG 
TCCTTTGTCG ACAATGGCTA CGACGACCTG GAGGTCTGCA AGCAGATCGG CGACCCGGAC 
CTGGATGCCA TCGGCGTGGC CGTGCCCCAG CACCGCCGCC GCATCCACGA GGCCGTGCGG 
CGGCTGCGGG AGGCGGACGA GCGGGCGGCG GGGCTCTACT TCACCCTGGA GCCGCCGCCC 
GCGCCTCCCG CCAAGGGGCC CCGCCGCCCG CCGGGGCAGG ACGGGGCGGC CCCCCGGCAC 
CGGCCTGGCC GCGGGGGCCC GGTGGTCTAC CCCAGGCTGA AGCTGAAGAT CATGATCCGG 
GACAAGCTGG TCCGCGACGG CATCAACCTG AGCAAGCCCC CCTACTCCAA CAAGGATGGG 
TCTCTGGGAA ACATTGATGA CCTCGCACAG CAGTATGCAG ATTATTACAA CACCTGCTTC 
ACGGATGTGT GTGAGAGGAT GGAAGAGCTG CGCAAACGGA GGGTTTCCCA GGACCTTGAT 
GTGGAGAAAC CTGATGCCAG TCCCACATCT CTACAACTGC GATCCCAGAT TGAGGAGTCA 
CTTGGTCTCA GCAGCACCGC ATCAACACCT GACACTGAAA GAAAGCTCTC CATTCATAAA 
TCAAGTTCGG AAGAAGGCTC TGTAGGGAAA GCAGACTGGA AAAAGAAAAA TAAGTTTTTT 
TGGCAAAATT TCCGAAAGAA CCAGAAAGGA TTGATGAGGC AGACTTCAAA AGGAGAGGAT 
GTGGGGTATG TGGCCAGTGA GATTACGATG AGCGATGAGG AACGGATCCA GCTGATGATG 
ATGGTGAAAG AGAAGATGAT CACTATTGAG GAAGCACTTG CTAGGCTGAA GGAGTATGAG 
TCCCAGCACA GGCAATCAAG TACTGTAGAT GCTACAGACT GGCCTGATGG TTCTTACTCA 
ACATTTGATG GGTCTTCCAA TTGTAATTCA AGAGAACAAT CCGATGATGA AACAGAGGAG 
TCGGTGAAGT TTAAGAGATT GCACAAGCTG GTAAATTCTA CTCGCAGAGT CAGGAAGAAA 
CTCATAAGGG TGGAAGAAAT GAAAAAACCC AGTACAGAAG GCGTAGAGGA GCAGTCGCTT 
GACAACTCTG CTATACTGGA TGACAGATCG GCACTTTACT CTGGAGTTCA CAAGAAGCAA 
TTCTACTTTG ATGGTTCCTC CGAGAAGCAG ACAGAGGATG ACTCGGACTC ACTTACTACT 
TCTCCCTCAT CTAGTAGTCT TGATACATGG GGAGCTAGCC GGAAACTGGT GAAGACCTTC 
AGCAAAACTG ATAGCCGTGG CCTTATCAAG CCTCCCAAGA AACTTGGGAC ATTTTTTTCT 
TACCCAGAAG ATGAGAAGAC CCAGAAAGTT TGCCGCTCCT TGACAGATGG GGAGATGAAG 
AAGAGCCTCG GCTCACTGAG CCATGGGAGA ACCTGCAGCT TTGGAGGATT TGACTTGACA 
AATCGTTCCC TTCATATTGG AAACAATTCT GACCAAATGG GCAAAGAAGG TGACTTTGTT 
TATAAAGAAG TAATCAAATC ACCCTCTGCC TCCCGTATAT CTCTGGGCAA AAAGGTGAAA 
TCTGTGAAAG AAACCATGAA GAAAAGGATG TCTAAAAAAT ACAGCAGCTC TCTGTCTGAG 
CAGGAGTCGA GCCCTGGAGT CATACCGGGT TCTCCGCAGT CGCCGCCGCC AGACACTGAC 
TCTCTGGACA AGCCCAAGCT GAAAGCTGGT GGCTCAGTTG AGAGCCTGAG GAGTTCCTTA 
AGTGGTCAGA GCTCAATGAG TGGTCAGACA GTGAGTACAA CAGATTCCTC AACCAGCAAC 
AGGGAGAGCG TGAAATCAGA AGATGGGGAT GATGAAGAAC CTCCCTACAG AGGACCCTTC 
TGTGGAAGAG CCAGGGTTCA CACTGATTTT ACTCCAAGTC CTTATGATAC AGACTCTCTG 
AAGCTCAAGA AAGGTGATAT CATTGACATC ATCAGCAAAC CCCCCATGGG CACTTGGATG 
GGCCTATTGA ACAACAAAGT TGGCACTTTC AAATTTATCT ATGTGGATGT GTTAAATGAA 
GAGGAAGAGA AGCCGAAGCG ACCCACCAGG AGACGCCGAA AAAGCAGACC ACCCCAACCC 
AAGTCAGTTG AGGATCTCTT GGATCGCATC AACTTAAAGG AGCACATGCC CACTTTTCTG 
TTCAATGGTT ATGAAGATCT GGATACCTTT AAGCTTTTAG AAGAAGAGGA TTTGGATGAA 
CTGAACATCC GAGATCCTGA GCACAGAGCT GTTCTCCTCA CAGCCGTGGA ACATCTACAG 
GAGTATGATA GTAACAGCGA CCAGTCAGGA TCTCAGGAGA AACTTCTCAT TGAAGGTCAA 
GGCCTAGCTG GATGCTCTCC TCGGGATTCT GGCTGCTATG AAAGCAGTGA AAACCTGGAG 
AATGGTAAAA CCCGAAGGAC CTGTCTTCTG CCCTCAAAAT CATCAAGTGA GCATAGCTTT 
AAAGACTTCA GCAGACACCA GCTATCAAAT TATCCCACAC TCCCACTGAC CAAGTCAATA 
GAAACTCTAC AGCAAGGAGA AAAAGAAAGC CGGTTGAGCT GTGCACATCG TGCTCTGAAG 
AGCTCTGTCA AGACCCCAGC TGTTATGGGT CTGAAGAAGA ACCGTAGAAG TTTACCAGTT 
GCTGTCTGTC GGAGCTATGA AACTCTGGAT GGCCCCCAGA GTGTGGATGT ATGGCCAAGA 
TCTCACTCCC TGGACGATCT CCAGGGAGAT TCCGATACTA ATCTACAAGA TGGTAGCAAG 
AAAGATTATT TTGCTCGGGA TTCACTGGAT ACAGCGAAAA AGGCAACTGG ATCTGCCCTG 
CCACCTCAGT CGTGTGGGGG CAGTTGTATG GTCGATAACT TGATGCCTAA GCAGGGTAAA 
GCAGCTGCTG GTTCTCAGAA GGGTAGAGGT AAGGAGTTGG AGTGCTCTGT AATGGAGAGT 
GCAGTTGGGA AACCTTTTCC ATTCCCCATG AAACACTGTG AAGCTCAGGC TGCCTTGGTG 
ACACATTCTG CAACAAGGAC ACCTCTGGAA ATACAGAGTA AGGGCTTTCA TGACCTTGCA 
CGGGCTGACT ATGCTCCAGT CCTCAAGGGA GGTCTAGAAG CTGAGCAAAA AGGCACAAAT 
GAGACAAGAA TGCAACCAAA AAATCCCTCT CAGCCGCCAC CTGTTCCTGC CAAAAAATGC 
CGAGAACGCC TTACTAATGG ATTATATCAT CCTCCCATGA CCCCCAGTGG GAACAACTCA 
AGTCAAGAAG CACCGTGTTT GCCAGTAAAA AAGACCAGTG CATCCAATCC CACTGATTTT 
CATGGAGTAC CTGTCCATAG GACATCTGAA CAGGAGCCCA GTACCCCTCC TAGCTCACTG 
CCACCCTGGC TGTTGGAGCA ACCAGAGACT AAAAGCGTTC AGCAGCACGT GATAAAGCTA 
GGGCCTGCTT CAGCAAGGAA AGTCTCTTGT AGCAGGGGAA TGGATCTGGA AATGGTAATA 
GAAAACAAGT TGCAGTCTGA AGGTATTGAT CTTACTGAAG AACCATACTC AGACAAGCAT 
GGTCGCTGTG GCATTCCTGA GGCTTTGGTA CAGAGGTACT CCGAAGACTT AGACCAGCCT 
GAAAAGGATG TTGCTGCAAA CATGGACCAA ATCCGGGTAA AGCAGCTGAG GAAGCAGCAT 
CGCATGGCAA TTCCAAGTGG AGGGCTCACT GAAATTTGCC GAAAACCTGT ATCACCAGGA 
CTTATCACCT CCGTATCTGA CTGGCTGATT TCCATTGGCC TACCCATGTA TTCCAGCCCA 
CTCACAGAAG CAGGGTTCAA CACACTGAGC AAAGTGCCTT CTCTGTCACA AACTTGCCTT 
CAGGAAGCTG GCATCACAGA GGAAAGGCAC ATAAGCAAGC TCCTGGCAGC AGCAAGACTC 
TTCAAACCTC TCGATCCAGA CGCAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004935653.1
CDS	727..4716
Translation	MCTNIVYEWLKTLQLPQYAESFVDNGYDDLEVCKQIGDPDLDAIGVAVPQHRRRIHEAVRRLREADERAAGLYFTLEPPPAPPAKGPRRPPGQDGAAPRHRPGRGGPVVYPRLKLKIMIRDKLVRDGINLSKPPYSNKDGSLGNIDDLAQQYADYYNTCFTDVCERMEELRKRRVSQDLDVEKPDASPTSLQLRSQIEESLGLSSTASTPDTERKLSIHKSSSEEGSVGKADWKKKNKFFWQNFRKNQKGLMRQTSKGEDVGYVASEITMSDEERIQLMMMVKEKMITIEEALARLKEYESQHRQSSTVDATDWPDGSYSTFDGSSNCNSREQSDDETEESVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGVEEQSLDNSAILDDRSALYSGVHKKQFYFDGSSEKQTEDDSDSLTTSPSSSSLDTWGASRKLVKTFSKTDSRGLIKPPKKLGTFFSYPEDEKTQKVCRSLTDGEMKKSLGSLSHGRTCSFGGFDLTNRSLHIGNNSDQMGKEGDFVYKEVIKSPSASRISLGKKVKSVKETMKKRMSKKYSSSLSEQESSPGVIPGSPQSPPPDTDSLDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRRKSRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVEHLQEYDSNSDQSGSQEKLLIEGQGLAGCSPRDSGCYESSENLENGKTRRTCLLPSKSSSEHSFKDFSRHQLSNYPTLPLTKSIETLQQGEKESRLSCAHRALKSSVKTPAVMGLKKNRRSLPVAVCRSYETLDGPQSVDVWPRSHSLDDLQGDSDTNLQDGSKKDYFARDSLDTAKKATGSALPPQSCGGSCMVDNLMPKQGKAAAGSQKGRGKELECSVMESAVGKPFPFPMKHCEAQAALVTHSATRTPLEIQSKGFHDLARADYAPVLKGGLEAEQKGTNETRMQPKNPSQPPPVPAKKCRERLTNGLYHPPMTPSGNNSSQEAPCLPVKKTSASNPTDFHGVPVHRTSEQEPSTPPSSLPPWLLEQPETKSVQQHVIKLGPASARKVSCSRGMDLEMVIENKLQSEGIDLTEEPYSDKHGRCGIPEALVQRYSEDLDQPEKDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGLITSVSDWLISIGLPMYSSPLTEAGFNTLSKVPSLSQTCLQEAGITEERHISKLLAAARLFKPLDPDAI

Target ORF information:

RefSeq Version	XM_004935596.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004935596.2

ORF Insert Sequence:

ATGTGCACCA ACATAGTGTA CGAGTGGCTG AAAACCCTGC AGCTCCCCCA GTACGCCGAG 
TCCTTTGTCG ACAATGGCTA CGACGACCTG GAGGTCTGCA AGCAGATCGG CGACCCGGAC 
CTGGATGCCA TCGGCGTGGC CGTGCCCCAG CACCGCCGCC GCATCCACGA GGCCGTGCGG 
CGGCTGCGGG AGGCGGACGA GCGGGCGGCG GGGCTCTACT TCACCCTGGA GCCGCCGCCC 
GCGCCTCCCG CCAAGGGGCC CCGCCGCCCG CCGGGGCAGG ACGGGGCGGC CCCCCGGCAC 
CGGCCTGGCC GCGGGGGCCC GGTGGTCTAC CCCAGGCTGA AGCTGAAGAT CATGATCCGG 
GACAAGCTGG TCCGCGACGG CATCAACCTG AGCAAGCCCC CCTACTCCAA CAAGGATGGG 
TCTCTGGGAA ACATTGATGA CCTCGCACAG CAGTATGCAG ATTATTACAA CACCTGCTTC 
ACGGATGTGT GTGAGAGGAT GGAAGAGCTG CGCAAACGGA GGGTTTCCCA GGACCTTGAT 
GTGGAGAAAC CTGATGCCAG TCCCACATCT CTACAACTGC GATCCCAGAT TGAGGAGTCA 
CTTGGTCTCA GCAGCACCGC ATCAACACCT GACACTGAAA GAAAGCTCTC CATTCATAAA 
TCAAGTTCGG AAGAAGGCTC TGTAGGGAAA GCAGACTGGA AAAAGAAAAA TAAGTTTTTT 
TGGCAAAATT TCCGAAAGAA CCAGAAAGGA TTGATGAGGC AGACTTCAAA AGGAGAGGAT 
GTGGGGTATG TGGCCAGTGA GATTACGATG AGCGATGAGG AACGGATCCA GCTGATGATG 
ATGGTGAAAG AGAAGATGAT CACTATTGAG GAAGCACTTG CTAGGCTGAA GGAGTATGAG 
TCCCAGCACA GGCAATCAAG TACTGTAGAT GCTACAGACT GGCCTGATGG TTCTTACTCA 
ACATTTGATG GGTCTTCCAA TTGTAATTCA AGAGAACAAT CCGATGATGA AACAGAGGAG 
TCGGTGAAGT TTAAGAGATT GCACAAGCTG GTAAATTCTA CTCGCAGAGT CAGGAAGAAA 
CTCATAAGGG TGGAAGAAAT GAAAAAACCC AGTACAGAAG GCGTAGAGGA GCAGTCGCTT 
GACAACTCTG CTATACTGGA TGACAGATCG GCACTTTACT CTGGAGTTCA CAAGAAGCAA 
TTCTACTTTG ATGGTTCCTC CGAGAAGCAG ACAGAGGATG ACTCGGACTC ACTTACTACT 
TCTCCCTCAT CTAGTAGTCT TGATACATGG GGAGCTAGCC GGAAACTGGT GAAGACCTTC 
AGCAAAACTG ATAGCCGTGG CCTTATCAAG CCTCCCAAGA AACTTGGGAC ATTTTTTTCT 
TACCCAGAAG ATGAGAAGAC CCAGAAAGTT TGCCGCTCCT TGACAGATGG GGAGATGAAG 
AAGAGCCTCG GCTCACTGAG CCATGGGAGA ACCTGCAGCT TTGGAGGATT TGACTTGACA 
AATCGTTCCC TTCATATTGG AAACAATTCT GACCAAATGG GCAAAGAAGG TGACTTTGTT 
TATAAAGAAG TAATCAAATC ACCCTCTGCC TCCCGTATAT CTCTGGGCAA AAAGGTGAAA 
TCTGTGAAAG AAACCATGAA GAAAAGGATG TCTAAAAAAT ACAGCAGCTC TCTGTCTGAG 
CAGGAGTCGA GCCCTGGAGT CATACCGGGT TCTCCGCAGT CGCCGCCGCC AGACACTGAC 
TCTCTGGACA AGCCCAAGCT GAAAGCTGGT GGCTCAGTTG AGAGCCTGAG GAGTTCCTTA 
AGTGGTCAGA GCTCAATGAG TGGTCAGACA GTGAGTACAA CAGATTCCTC AACCAGCAAC 
AGGGAGAGCG TGAAATCAGA AGATGGGGAT GATGAAGAAC CTCCCTACAG AGGACCCTTC 
TGTGGAAGAG CCAGGGTTCA CACTGATTTT ACTCCAAGTC CTTATGATAC AGACTCTCTG 
AAGCTCAAGA AAGGTGATAT CATTGACATC ATCAGCAAAC CCCCCATGGG CACTTGGATG 
GGCCTATTGA ACAACAAAGT TGGCACTTTC AAATTTATCT ATGTGGATGT GTTAAATGAA 
GAGGAAGAGA AGCCGAAGCG ACCCACCAGG AGACGCCGAA AAAGCAGACC ACCCCAACCC 
AAGTCAGTTG AGGATCTCTT GGATCGCATC AACTTAAAGG AGCACATGCC CACTTTTCTG 
TTCAATGGTT ATGAAGATCT GGATACCTTT AAGCTTTTAG AAGAAGAGGA TTTGGATGAA 
CTGAACATCC GAGATCCTGA GCACAGAGCT GTTCTCCTCA CAGCCGTGGA ACATCTACAG 
GAGTATGATA GTAACAGCGA CCAGTCAGGA TCTCAGGAGA AACTTCTCAT TGAAGGTCAA 
GGCCTAGCTG GATGCTCTCC TCGGGATTCT GGCTGCTATG AAAGCAGTGA AAACCTGGAG 
AATGGTAAAA CCCGAAGGAC CTGTCTTCTG CCCTCAAAAT CATCAAGTGA GCATAGCTTT 
AAAGACTTCA GCAGACACCA GCTATCAAAT TATCCCACAC TCCCACTGAC CAAGTCAATA 
GAAACTCTAC AGCAAGGAGA AAAAGAAAGC CGGTTGAGCT GTGCACATCG TGCTCTGAAG 
AGCTCTGTCA AGACCCCAGC TGTTATGGGT CTGAAGAAGA ACCGTAGAAG TTTACCAGTT 
GCTGTCTGTC GGAGCTATGA AACTCTGGAT GGCCCCCAGA GTGTGGATGT ATGGCCAAGA 
TCTCACTCCC TGGACGATCT CCAGGGAGAT TCCGATACTA ATCTACAAGA TGGTAGCAAG 
AAAGATTATT TTGCTCGGGA TTCACTGGAT ACAGCGAAAA AGGCAACTGG ATCTGCCCTG 
CCACCTCAGT CGTGTGGGGG CAGTTGTATG GTCGATAACT TGATGCCTAA GCAGGGTAAA 
GCAGCTGCTG GTTCTCAGAA GGGTAGAGGT AAGGAGTTGG AGTGCTCTGT AATGGAGAGT 
GCAGTTGGGA AACCTTTTCC ATTCCCCATG AAACACTGTG AAGCTCAGGC TGCCTTGGTG 
ACACATTCTG CAACAAGGAC ACCTCTGGAA ATACAGAGTA AGGGCTTTCA TGACCTTGCA 
CGGGCTGACT ATGCTCCAGT CCTCAAGGGA GGTCTAGAAG CTGAGCAAAA AGGCACAAAT 
GAGACAAGAA TGCAACCAAA AAATCCCTCT CAGCCGCCAC CTGTTCCTGC CAAAAAATGC 
CGAGAACGCC TTACTAATGG ATTATATCAT CCTCCCATGA CCCCCAGTGG GAACAACTCA 
AGTCAAGAAG CACCGTGTTT GCCAGTAAAA AAGACCAGTG CATCCAATCC CACTGATTTT 
CATGGAGTAC CTGTCCATAG GACATCTGAA CAGGAGCCCA GTACCCCTCC TAGCTCACTG 
CCACCCTGGC TGTTGGAGCA ACCAGAGACT AAAAGCGTTC AGCAGCACGT GATAAAGCTA 
GGGCCTGCTT CAGCAAGGAA AGTCTCTTGT AGCAGGGGAA TGGATCTGGA AATGGTAATA 
GAAAACAAGT TGCAGTCTGA AGGTATTGAT CTTACTGAAG AACCATACTC AGACAAGCAT 
GGTCGCTGTG GCATTCCTGA GGCTTTGGTA CAGAGGTACT CCGAAGACTT AGACCAGCCT 
GAAAAGGATG TTGCTGCAAA CATGGACCAA ATCCGGGTAA AGCAGCTGAG GAAGCAGCAT 
CGCATGGCAA TTCCAAGTGG AGGGCTCACT GAAATTTGCC GAAAACCTGT ATCACCAGGA 
CTTATCACCT CCGTATCTGA CTGGCTGATT TCCATTGGCC TACCCATGTA TTCCAGCCCA 
CTCACAGAAG CAGGGTTCAA CACACTGAGC AAAGTGCCTT CTCTGTCACA AACTTGCCTT 
CAGGAAGCTG GCATCACAGA GGAAAGGCAC ATAAGCAAGC TCCTGGCAGC AGCAAGACTC 
TTCAAACCTC TCGATCCAGA CGCAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH1 ( XM_025149372.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005140.1 Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X4, mRNA.

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CloneID	OGa06281
Clone ID Related Accession (Same CDS sequence)	XM_004935596.2 , XM_025149372.1
Accession Version	XM_025149372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	SAM and SH3 domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACCA ACATAGTGTA CGAGTGGCTG AAAACCCTGC AGCTCCCCCA GTACGCCGAG 
TCCTTTGTCG ACAATGGCTA CGACGACCTG GAGGTCTGCA AGCAGATCGG CGACCCGGAC 
CTGGATGCCA TCGGCGTGGC CGTGCCCCAG CACCGCCGCC GCATCCACGA GGCCGTGCGG 
CGGCTGCGGG AGGCGGACGA GCGGGCGGCG GGGCTCTACT TCACCCTGGA GCCGCCGCCC 
GCGCCTCCCG CCAAGGGGCC CCGCCGCCCG CCGGGGCAGG ACGGGGCGGC CCCCCGGCAC 
CGGCCTGGCC GCGGGGGCCC GGTGGTCTAC CCCAGGCTGA AGCTGAAGAT CATGATCCGG 
GACAAGCTGG TCCGCGACGG CATCAACCTG AGCAAGCCCC CCTACTCCAA CAAGGATGGG 
TCTCTGGGAA ACATTGATGA CCTCGCACAG CAGTATGCAG ATTATTACAA CACCTGCTTC 
ACGGATGTGT GTGAGAGGAT GGAAGAGCTG CGCAAACGGA GGGTTTCCCA GGACCTTGAT 
GTGGAGAAAC CTGATGCCAG TCCCACATCT CTACAACTGC GATCCCAGAT TGAGGAGTCA 
CTTGGTCTCA GCAGCACCGC ATCAACACCT GACACTGAAA GAAAGCTCTC CATTCATAAA 
TCAAGTTCGG AAGAAGGCTC TGTAGGGAAA GCAGACTGGA AAAAGAAAAA TAAGTTTTTT 
TGGCAAAATT TCCGAAAGAA CCAGAAAGGA TTGATGAGGC AGACTTCAAA AGGAGAGGAT 
GTGGGGTATG TGGCCAGTGA GATTACGATG AGCGATGAGG AACGGATCCA GCTGATGATG 
ATGGTGAAAG AGAAGATGAT CACTATTGAG GAAGCACTTG CTAGGCTGAA GGAGTATGAG 
TCCCAGCACA GGCAATCAAG TACTGTAGAT GCTACAGACT GGCCTGATGG TTCTTACTCA 
ACATTTGATG GGTCTTCCAA TTGTAATTCA AGAGAACAAT CCGATGATGA AACAGAGGAG 
TCGGTGAAGT TTAAGAGATT GCACAAGCTG GTAAATTCTA CTCGCAGAGT CAGGAAGAAA 
CTCATAAGGG TGGAAGAAAT GAAAAAACCC AGTACAGAAG GCGTAGAGGA GCAGTCGCTT 
GACAACTCTG CTATACTGGA TGACAGATCG GCACTTTACT CTGGAGTTCA CAAGAAGCAA 
TTCTACTTTG ATGGTTCCTC CGAGAAGCAG ACAGAGGATG ACTCGGACTC ACTTACTACT 
TCTCCCTCAT CTAGTAGTCT TGATACATGG GGAGCTAGCC GGAAACTGGT GAAGACCTTC 
AGCAAAACTG ATAGCCGTGG CCTTATCAAG CCTCCCAAGA AACTTGGGAC ATTTTTTTCT 
TACCCAGAAG ATGAGAAGAC CCAGAAAGTT TGCCGCTCCT TGACAGATGG GGAGATGAAG 
AAGAGCCTCG GCTCACTGAG CCATGGGAGA ACCTGCAGCT TTGGAGGATT TGACTTGACA 
AATCGTTCCC TTCATATTGG AAACAATTCT GACCAAATGG GCAAAGAAGG TGACTTTGTT 
TATAAAGAAG TAATCAAATC ACCCTCTGCC TCCCGTATAT CTCTGGGCAA AAAGGTGAAA 
TCTGTGAAAG AAACCATGAA GAAAAGGATG TCTAAAAAAT ACAGCAGCTC TCTGTCTGAG 
CAGGAGTCGA GCCCTGGAGT CATACCGGGT TCTCCGCAGT CGCCGCCGCC AGACACTGAC 
TCTCTGGACA AGCCCAAGCT GAAAGCTGGT GGCTCAGTTG AGAGCCTGAG GAGTTCCTTA 
AGTGGTCAGA GCTCAATGAG TGGTCAGACA GTGAGTACAA CAGATTCCTC AACCAGCAAC 
AGGGAGAGCG TGAAATCAGA AGATGGGGAT GATGAAGAAC CTCCCTACAG AGGACCCTTC 
TGTGGAAGAG CCAGGGTTCA CACTGATTTT ACTCCAAGTC CTTATGATAC AGACTCTCTG 
AAGCTCAAGA AAGGTGATAT CATTGACATC ATCAGCAAAC CCCCCATGGG CACTTGGATG 
GGCCTATTGA ACAACAAAGT TGGCACTTTC AAATTTATCT ATGTGGATGT GTTAAATGAA 
GAGGAAGAGA AGCCGAAGCG ACCCACCAGG AGACGCCGAA AAAGCAGACC ACCCCAACCC 
AAGTCAGTTG AGGATCTCTT GGATCGCATC AACTTAAAGG AGCACATGCC CACTTTTCTG 
TTCAATGGTT ATGAAGATCT GGATACCTTT AAGCTTTTAG AAGAAGAGGA TTTGGATGAA 
CTGAACATCC GAGATCCTGA GCACAGAGCT GTTCTCCTCA CAGCCGTGGA ACATCTACAG 
GAGTATGATA GTAACAGCGA CCAGTCAGGA TCTCAGGAGA AACTTCTCAT TGAAGGTCAA 
GGCCTAGCTG GATGCTCTCC TCGGGATTCT GGCTGCTATG AAAGCAGTGA AAACCTGGAG 
AATGGTAAAA CCCGAAGGAC CTGTCTTCTG CCCTCAAAAT CATCAAGTGA GCATAGCTTT 
AAAGACTTCA GCAGACACCA GCTATCAAAT TATCCCACAC TCCCACTGAC CAAGTCAATA 
GAAACTCTAC AGCAAGGAGA AAAAGAAAGC CGGTTGAGCT GTGCACATCG TGCTCTGAAG 
AGCTCTGTCA AGACCCCAGC TGTTATGGGT CTGAAGAAGA ACCGTAGAAG TTTACCAGTT 
GCTGTCTGTC GGAGCTATGA AACTCTGGAT GGCCCCCAGA GTGTGGATGT ATGGCCAAGA 
TCTCACTCCC TGGACGATCT CCAGGGAGAT TCCGATACTA ATCTACAAGA TGGTAGCAAG 
AAAGATTATT TTGCTCGGGA TTCACTGGAT ACAGCGAAAA AGGCAACTGG ATCTGCCCTG 
CCACCTCAGT CGTGTGGGGG CAGTTGTATG GTCGATAACT TGATGCCTAA GCAGGGTAAA 
GCAGCTGCTG GTTCTCAGAA GGGTAGAGGT AAGGAGTTGG AGTGCTCTGT AATGGAGAGT 
GCAGTTGGGA AACCTTTTCC ATTCCCCATG AAACACTGTG AAGCTCAGGC TGCCTTGGTG 
ACACATTCTG CAACAAGGAC ACCTCTGGAA ATACAGAGTA AGGGCTTTCA TGACCTTGCA 
CGGGCTGACT ATGCTCCAGT CCTCAAGGGA GGTCTAGAAG CTGAGCAAAA AGGCACAAAT 
GAGACAAGAA TGCAACCAAA AAATCCCTCT CAGCCGCCAC CTGTTCCTGC CAAAAAATGC 
CGAGAACGCC TTACTAATGG ATTATATCAT CCTCCCATGA CCCCCAGTGG GAACAACTCA 
AGTCAAGAAG CACCGTGTTT GCCAGTAAAA AAGACCAGTG CATCCAATCC CACTGATTTT 
CATGGAGTAC CTGTCCATAG GACATCTGAA CAGGAGCCCA GTACCCCTCC TAGCTCACTG 
CCACCCTGGC TGTTGGAGCA ACCAGAGACT AAAAGCGTTC AGCAGCACGT GATAAAGCTA 
GGGCCTGCTT CAGCAAGGAA AGTCTCTTGT AGCAGGGGAA TGGATCTGGA AATGGTAATA 
GAAAACAAGT TGCAGTCTGA AGGTATTGAT CTTACTGAAG AACCATACTC AGACAAGCAT 
GGTCGCTGTG GCATTCCTGA GGCTTTGGTA CAGAGGTACT CCGAAGACTT AGACCAGCCT 
GAAAAGGATG TTGCTGCAAA CATGGACCAA ATCCGGGTAA AGCAGCTGAG GAAGCAGCAT 
CGCATGGCAA TTCCAAGTGG AGGGCTCACT GAAATTTGCC GAAAACCTGT ATCACCAGGA 
CTTATCACCT CCGTATCTGA CTGGCTGATT TCCATTGGCC TACCCATGTA TTCCAGCCCA 
CTCACAGAAG CAGGGTTCAA CACACTGAGC AAAGTGCCTT CTCTGTCACA AACTTGCCTT 
CAGGAAGCTG GCATCACAGA GGAAAGGCAC ATAAGCAAGC TCCTGGCAGC AGCAAGACTC 
TTCAAACCTC TCGATCCAGA CGCAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005140.1
CDS	131..4120
Translation	MCTNIVYEWLKTLQLPQYAESFVDNGYDDLEVCKQIGDPDLDAIGVAVPQHRRRIHEAVRRLREADERAAGLYFTLEPPPAPPAKGPRRPPGQDGAAPRHRPGRGGPVVYPRLKLKIMIRDKLVRDGINLSKPPYSNKDGSLGNIDDLAQQYADYYNTCFTDVCERMEELRKRRVSQDLDVEKPDASPTSLQLRSQIEESLGLSSTASTPDTERKLSIHKSSSEEGSVGKADWKKKNKFFWQNFRKNQKGLMRQTSKGEDVGYVASEITMSDEERIQLMMMVKEKMITIEEALARLKEYESQHRQSSTVDATDWPDGSYSTFDGSSNCNSREQSDDETEESVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGVEEQSLDNSAILDDRSALYSGVHKKQFYFDGSSEKQTEDDSDSLTTSPSSSSLDTWGASRKLVKTFSKTDSRGLIKPPKKLGTFFSYPEDEKTQKVCRSLTDGEMKKSLGSLSHGRTCSFGGFDLTNRSLHIGNNSDQMGKEGDFVYKEVIKSPSASRISLGKKVKSVKETMKKRMSKKYSSSLSEQESSPGVIPGSPQSPPPDTDSLDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRRKSRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVEHLQEYDSNSDQSGSQEKLLIEGQGLAGCSPRDSGCYESSENLENGKTRRTCLLPSKSSSEHSFKDFSRHQLSNYPTLPLTKSIETLQQGEKESRLSCAHRALKSSVKTPAVMGLKKNRRSLPVAVCRSYETLDGPQSVDVWPRSHSLDDLQGDSDTNLQDGSKKDYFARDSLDTAKKATGSALPPQSCGGSCMVDNLMPKQGKAAAGSQKGRGKELECSVMESAVGKPFPFPMKHCEAQAALVTHSATRTPLEIQSKGFHDLARADYAPVLKGGLEAEQKGTNETRMQPKNPSQPPPVPAKKCRERLTNGLYHPPMTPSGNNSSQEAPCLPVKKTSASNPTDFHGVPVHRTSEQEPSTPPSSLPPWLLEQPETKSVQQHVIKLGPASARKVSCSRGMDLEMVIENKLQSEGIDLTEEPYSDKHGRCGIPEALVQRYSEDLDQPEKDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGLITSVSDWLISIGLPMYSSPLTEAGFNTLSKVPSLSQTCLQEAGITEERHISKLLAAARLFKPLDPDAI

Target ORF information:

RefSeq Version	XM_025149372.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149372.1

ORF Insert Sequence:

ATGTGCACCA ACATAGTGTA CGAGTGGCTG AAAACCCTGC AGCTCCCCCA GTACGCCGAG 
TCCTTTGTCG ACAATGGCTA CGACGACCTG GAGGTCTGCA AGCAGATCGG CGACCCGGAC 
CTGGATGCCA TCGGCGTGGC CGTGCCCCAG CACCGCCGCC GCATCCACGA GGCCGTGCGG 
CGGCTGCGGG AGGCGGACGA GCGGGCGGCG GGGCTCTACT TCACCCTGGA GCCGCCGCCC 
GCGCCTCCCG CCAAGGGGCC CCGCCGCCCG CCGGGGCAGG ACGGGGCGGC CCCCCGGCAC 
CGGCCTGGCC GCGGGGGCCC GGTGGTCTAC CCCAGGCTGA AGCTGAAGAT CATGATCCGG 
GACAAGCTGG TCCGCGACGG CATCAACCTG AGCAAGCCCC CCTACTCCAA CAAGGATGGG 
TCTCTGGGAA ACATTGATGA CCTCGCACAG CAGTATGCAG ATTATTACAA CACCTGCTTC 
ACGGATGTGT GTGAGAGGAT GGAAGAGCTG CGCAAACGGA GGGTTTCCCA GGACCTTGAT 
GTGGAGAAAC CTGATGCCAG TCCCACATCT CTACAACTGC GATCCCAGAT TGAGGAGTCA 
CTTGGTCTCA GCAGCACCGC ATCAACACCT GACACTGAAA GAAAGCTCTC CATTCATAAA 
TCAAGTTCGG AAGAAGGCTC TGTAGGGAAA GCAGACTGGA AAAAGAAAAA TAAGTTTTTT 
TGGCAAAATT TCCGAAAGAA CCAGAAAGGA TTGATGAGGC AGACTTCAAA AGGAGAGGAT 
GTGGGGTATG TGGCCAGTGA GATTACGATG AGCGATGAGG AACGGATCCA GCTGATGATG 
ATGGTGAAAG AGAAGATGAT CACTATTGAG GAAGCACTTG CTAGGCTGAA GGAGTATGAG 
TCCCAGCACA GGCAATCAAG TACTGTAGAT GCTACAGACT GGCCTGATGG TTCTTACTCA 
ACATTTGATG GGTCTTCCAA TTGTAATTCA AGAGAACAAT CCGATGATGA AACAGAGGAG 
TCGGTGAAGT TTAAGAGATT GCACAAGCTG GTAAATTCTA CTCGCAGAGT CAGGAAGAAA 
CTCATAAGGG TGGAAGAAAT GAAAAAACCC AGTACAGAAG GCGTAGAGGA GCAGTCGCTT 
GACAACTCTG CTATACTGGA TGACAGATCG GCACTTTACT CTGGAGTTCA CAAGAAGCAA 
TTCTACTTTG ATGGTTCCTC CGAGAAGCAG ACAGAGGATG ACTCGGACTC ACTTACTACT 
TCTCCCTCAT CTAGTAGTCT TGATACATGG GGAGCTAGCC GGAAACTGGT GAAGACCTTC 
AGCAAAACTG ATAGCCGTGG CCTTATCAAG CCTCCCAAGA AACTTGGGAC ATTTTTTTCT 
TACCCAGAAG ATGAGAAGAC CCAGAAAGTT TGCCGCTCCT TGACAGATGG GGAGATGAAG 
AAGAGCCTCG GCTCACTGAG CCATGGGAGA ACCTGCAGCT TTGGAGGATT TGACTTGACA 
AATCGTTCCC TTCATATTGG AAACAATTCT GACCAAATGG GCAAAGAAGG TGACTTTGTT 
TATAAAGAAG TAATCAAATC ACCCTCTGCC TCCCGTATAT CTCTGGGCAA AAAGGTGAAA 
TCTGTGAAAG AAACCATGAA GAAAAGGATG TCTAAAAAAT ACAGCAGCTC TCTGTCTGAG 
CAGGAGTCGA GCCCTGGAGT CATACCGGGT TCTCCGCAGT CGCCGCCGCC AGACACTGAC 
TCTCTGGACA AGCCCAAGCT GAAAGCTGGT GGCTCAGTTG AGAGCCTGAG GAGTTCCTTA 
AGTGGTCAGA GCTCAATGAG TGGTCAGACA GTGAGTACAA CAGATTCCTC AACCAGCAAC 
AGGGAGAGCG TGAAATCAGA AGATGGGGAT GATGAAGAAC CTCCCTACAG AGGACCCTTC 
TGTGGAAGAG CCAGGGTTCA CACTGATTTT ACTCCAAGTC CTTATGATAC AGACTCTCTG 
AAGCTCAAGA AAGGTGATAT CATTGACATC ATCAGCAAAC CCCCCATGGG CACTTGGATG 
GGCCTATTGA ACAACAAAGT TGGCACTTTC AAATTTATCT ATGTGGATGT GTTAAATGAA 
GAGGAAGAGA AGCCGAAGCG ACCCACCAGG AGACGCCGAA AAAGCAGACC ACCCCAACCC 
AAGTCAGTTG AGGATCTCTT GGATCGCATC AACTTAAAGG AGCACATGCC CACTTTTCTG 
TTCAATGGTT ATGAAGATCT GGATACCTTT AAGCTTTTAG AAGAAGAGGA TTTGGATGAA 
CTGAACATCC GAGATCCTGA GCACAGAGCT GTTCTCCTCA CAGCCGTGGA ACATCTACAG 
GAGTATGATA GTAACAGCGA CCAGTCAGGA TCTCAGGAGA AACTTCTCAT TGAAGGTCAA 
GGCCTAGCTG GATGCTCTCC TCGGGATTCT GGCTGCTATG AAAGCAGTGA AAACCTGGAG 
AATGGTAAAA CCCGAAGGAC CTGTCTTCTG CCCTCAAAAT CATCAAGTGA GCATAGCTTT 
AAAGACTTCA GCAGACACCA GCTATCAAAT TATCCCACAC TCCCACTGAC CAAGTCAATA 
GAAACTCTAC AGCAAGGAGA AAAAGAAAGC CGGTTGAGCT GTGCACATCG TGCTCTGAAG 
AGCTCTGTCA AGACCCCAGC TGTTATGGGT CTGAAGAAGA ACCGTAGAAG TTTACCAGTT 
GCTGTCTGTC GGAGCTATGA AACTCTGGAT GGCCCCCAGA GTGTGGATGT ATGGCCAAGA 
TCTCACTCCC TGGACGATCT CCAGGGAGAT TCCGATACTA ATCTACAAGA TGGTAGCAAG 
AAAGATTATT TTGCTCGGGA TTCACTGGAT ACAGCGAAAA AGGCAACTGG ATCTGCCCTG 
CCACCTCAGT CGTGTGGGGG CAGTTGTATG GTCGATAACT TGATGCCTAA GCAGGGTAAA 
GCAGCTGCTG GTTCTCAGAA GGGTAGAGGT AAGGAGTTGG AGTGCTCTGT AATGGAGAGT 
GCAGTTGGGA AACCTTTTCC ATTCCCCATG AAACACTGTG AAGCTCAGGC TGCCTTGGTG 
ACACATTCTG CAACAAGGAC ACCTCTGGAA ATACAGAGTA AGGGCTTTCA TGACCTTGCA 
CGGGCTGACT ATGCTCCAGT CCTCAAGGGA GGTCTAGAAG CTGAGCAAAA AGGCACAAAT 
GAGACAAGAA TGCAACCAAA AAATCCCTCT CAGCCGCCAC CTGTTCCTGC CAAAAAATGC 
CGAGAACGCC TTACTAATGG ATTATATCAT CCTCCCATGA CCCCCAGTGG GAACAACTCA 
AGTCAAGAAG CACCGTGTTT GCCAGTAAAA AAGACCAGTG CATCCAATCC CACTGATTTT 
CATGGAGTAC CTGTCCATAG GACATCTGAA CAGGAGCCCA GTACCCCTCC TAGCTCACTG 
CCACCCTGGC TGTTGGAGCA ACCAGAGACT AAAAGCGTTC AGCAGCACGT GATAAAGCTA 
GGGCCTGCTT CAGCAAGGAA AGTCTCTTGT AGCAGGGGAA TGGATCTGGA AATGGTAATA 
GAAAACAAGT TGCAGTCTGA AGGTATTGAT CTTACTGAAG AACCATACTC AGACAAGCAT 
GGTCGCTGTG GCATTCCTGA GGCTTTGGTA CAGAGGTACT CCGAAGACTT AGACCAGCCT 
GAAAAGGATG TTGCTGCAAA CATGGACCAA ATCCGGGTAA AGCAGCTGAG GAAGCAGCAT 
CGCATGGCAA TTCCAAGTGG AGGGCTCACT GAAATTTGCC GAAAACCTGT ATCACCAGGA 
CTTATCACCT CCGTATCTGA CTGGCTGATT TCCATTGGCC TACCCATGTA TTCCAGCCCA 
CTCACAGAAG CAGGGTTCAA CACACTGAGC AAAGTGCCTT CTCTGTCACA AACTTGCCTT 
CAGGAAGCTG GCATCACAGA GGAAAGGCAC ATAAGCAAGC TCCTGGCAGC AGCAAGACTC 
TTCAAACCTC TCGATCCAGA CGCAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH1 ( XM_004935597.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004935654.1 Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa06282
Clone ID Related Accession (Same CDS sequence)	XM_004935597.2
Accession Version	XM_004935597.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	SAM and SH3 domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004935597.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACCA ACATAGTGTA CGAGTGGCTG AAAACCCTGC AGCTCCCCCA GTACGCCGAG 
TCCTTTGTCG ACAATGGCTA CGACGACCTG GAGGTCTGCA AGCAGATCGG CGACCCGGAC 
CTGGATGCCA TCGGCGTGGC CGTGCCCCAG CACCGCCGCC GCATCCACGA GGCCGTGCGG 
CGGCTGCGGG AGGCGGACGA GCGGGCGGCG GGGCTCTACT TCACCCTGGA GCCGCCGCCC 
GCGCCTCCCG CCAAGGGGCC CCGCCGCCCG CCGGGGCAGG ACGGGGCGGC CCCCCGGCAC 
CGGCCTGGCC GCGGGGGCCC GGTGGTCTAC CCCAGGCTGA AGCTGAAGAT CATGATCCGG 
GACAAGCTGG TCCGCGACGG CATCAACCTG AGCAAGCCCC CCTACTCCAA CAAGGATGGG 
TCTCTGGGAA ACATTGATGA CCTCGCACAG CAGTATGCAG ATTATTACAA CACCTGCTTC 
ACGGATGTGT GTGAGAGGAT GGAAGAGCTG CGCAAACGGA GGGTTTCCCA GGACCTTGAT 
GTGGAGAAAC CTGATGCCAG TCCCACATCT CTACAACTGC GATCCCAGAT TGAGGAGTCA 
CTTGGTCTCA GCAGCACCGC ATCAACACCT GACACTGAAA GAAAGCTCTC CATTCATAAA 
TCAAGTTCGG AAGAAGGCTC TGTAGACTGG AAAAAGAAAA ATAAGTTTTT TTGGCAAAAT 
TTCCGAAAGA ACCAGAAAGG ATTGATGAGG CAGACTTCAA AAGGAGAGGA TGTGGGGTAT 
GTGGCCAGTG AGATTACGAT GAGCGATGAG GAACGGATCC AGCTGATGAT GATGGTGAAA 
GAGAAGATGA TCACTATTGA GGAAGCACTT GCTAGGCTGA AGGAGTATGA GTCCCAGCAC 
AGGCAATCAA GTACTGTAGA TGCTACAGAC TGGCCTGATG GTTCTTACTC AACATTTGAT 
GGGTCTTCCA ATTGTAATTC AAGAGAACAA TCCGATGATG AAACAGAGGA GTCGGTGAAG 
TTTAAGAGAT TGCACAAGCT GGTAAATTCT ACTCGCAGAG TCAGGAAGAA ACTCATAAGG 
GTGGAAGAAA TGAAAAAACC CAGTACAGAA GGCGTAGAGG AGCAGTCGCT TGACAACTCT 
GCTATACTGG ATGACAGATC GGCACTTTAC TCTGGAGTTC ACAAGAAGCA ATTCTACTTT 
GATGGTTCCT CCGAGAAGCA GACAGAGGAT GACTCGGACT CACTTACTAC TTCTCCCTCA 
TCTAGTAGTC TTGATACATG GGGAGCTAGC CGGAAACTGG TGAAGACCTT CAGCAAAACT 
GATAGCCGTG GCCTTATCAA GCCTCCCAAG AAACTTGGGA CATTTTTTTC TTACCCAGAA 
GATGAGAAGA CCCAGAAAGT TTGCCGCTCC TTGACAGATG GGGAGATGAA GAAGAGCCTC 
GGCTCACTGA GCCATGGGAG AACCTGCAGC TTTGGAGGAT TTGACTTGAC AAATCGTTCC 
CTTCATATTG GAAACAATTC TGACCAAATG GGCAAAGAAG GTGACTTTGT TTATAAAGAA 
GTAATCAAAT CACCCTCTGC CTCCCGTATA TCTCTGGGCA AAAAGGTGAA ATCTGTGAAA 
GAAACCATGA AGAAAAGGAT GTCTAAAAAA TACAGCAGCT CTCTGTCTGA GCAGGAGTCG 
AGCCCTGGAG TCATACCGGG TTCTCCGCAG TCGCCGCCGC CAGACACTGA CTCTCTGGAC 
AAGCCCAAGC TGAAAGCTGG TGGCTCAGTT GAGAGCCTGA GGAGTTCCTT AAGTGGTCAG 
AGCTCAATGA GTGGTCAGAC AGTGAGTACA ACAGATTCCT CAACCAGCAA CAGGGAGAGC 
GTGAAATCAG AAGATGGGGA TGATGAAGAA CCTCCCTACA GAGGACCCTT CTGTGGAAGA 
GCCAGGGTTC ACACTGATTT TACTCCAAGT CCTTATGATA CAGACTCTCT GAAGCTCAAG 
AAAGGTGATA TCATTGACAT CATCAGCAAA CCCCCCATGG GCACTTGGAT GGGCCTATTG 
AACAACAAAG TTGGCACTTT CAAATTTATC TATGTGGATG TGTTAAATGA AGAGGAAGAG 
AAGCCGAAGC GACCCACCAG GAGACGCCGA AAAAGCAGAC CACCCCAACC CAAGTCAGTT 
GAGGATCTCT TGGATCGCAT CAACTTAAAG GAGCACATGC CCACTTTTCT GTTCAATGGT 
TATGAAGATC TGGATACCTT TAAGCTTTTA GAAGAAGAGG ATTTGGATGA ACTGAACATC 
CGAGATCCTG AGCACAGAGC TGTTCTCCTC ACAGCCGTGG AACATCTACA GGAGTATGAT 
AGTAACAGCG ACCAGTCAGG ATCTCAGGAG AAACTTCTCA TTGAAGGTCA AGGCCTAGCT 
GGATGCTCTC CTCGGGATTC TGGCTGCTAT GAAAGCAGTG AAAACCTGGA GAATGGTAAA 
ACCCGAAGGA CCTGTCTTCT GCCCTCAAAA TCATCAAGTG AGCATAGCTT TAAAGACTTC 
AGCAGACACC AGCTATCAAA TTATCCCACA CTCCCACTGA CCAAGTCAAT AGAAACTCTA 
CAGCAAGGAG AAAAAGAAAG CCGGTTGAGC TGTGCACATC GTGCTCTGAA GAGCTCTGTC 
AAGACCCCAG CTGTTATGGG TCTGAAGAAG AACCGTAGAA GTTTACCAGT TGCTGTCTGT 
CGGAGCTATG AAACTCTGGA TGGCCCCCAG AGTGTGGATG TATGGCCAAG ATCTCACTCC 
CTGGACGATC TCCAGGGAGA TTCCGATACT AATCTACAAG ATGGTAGCAA GAAAGATTAT 
TTTGCTCGGG ATTCACTGGA TACAGCGAAA AAGGCAACTG GATCTGCCCT GCCACCTCAG 
TCGTGTGGGG GCAGTTGTAT GGTCGATAAC TTGATGCCTA AGCAGGGTAA AGCAGCTGCT 
GGTTCTCAGA AGGGTAGAGG TAAGGAGTTG GAGTGCTCTG TAATGGAGAG TGCAGTTGGG 
AAACCTTTTC CATTCCCCAT GAAACACTGT GAAGCTCAGG CTGCCTTGGT GACACATTCT 
GCAACAAGGA CACCTCTGGA AATACAGAGT AAGGGCTTTC ATGACCTTGC ACGGGCTGAC 
TATGCTCCAG TCCTCAAGGG AGGTCTAGAA GCTGAGCAAA AAGGCACAAA TGAGACAAGA 
ATGCAACCAA AAAATCCCTC TCAGCCGCCA CCTGTTCCTG CCAAAAAATG CCGAGAACGC 
CTTACTAATG GATTATATCA TCCTCCCATG ACCCCCAGTG GGAACAACTC AAGTCAAGAA 
GCACCGTGTT TGCCAGTAAA AAAGACCAGT GCATCCAATC CCACTGATTT TCATGGAGTA 
CCTGTCCATA GGACATCTGA ACAGGAGCCC AGTACCCCTC CTAGCTCACT GCCACCCTGG 
CTGTTGGAGC AACCAGAGAC TAAAAGCGTT CAGCAGCACG TGATAAAGCT AGGGCCTGCT 
TCAGCAAGGA AAGTCTCTTG TAGCAGGGGA ATGGATCTGG AAATGGTAAT AGAAAACAAG 
TTGCAGTCTG AAGGTATTGA TCTTACTGAA GAACCATACT CAGACAAGCA TGGTCGCTGT 
GGCATTCCTG AGGCTTTGGT ACAGAGGTAC TCCGAAGACT TAGACCAGCC TGAAAAGGAT 
GTTGCTGCAA ACATGGACCA AATCCGGGTA AAGCAGCTGA GGAAGCAGCA TCGCATGGCA 
ATTCCAAGTG GAGGGCTCAC TGAAATTTGC CGAAAACCTG TATCACCAGG ACTTATCACC 
TCCGTATCTG ACTGGCTGAT TTCCATTGGC CTACCCATGT ATTCCAGCCC ACTCACAGAA 
GCAGGGTTCA ACACACTGAG CAAAGTGCCT TCTCTGTCAC AAACTTGCCT TCAGGAAGCT 
GGCATCACAG AGGAAAGGCA CATAAGCAAG CTCCTGGCAG CAGCAAGACT CTTCAAACCT 
CTCGATCCAG ACGCAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004935654.1
CDS	728..4708
Translation	MCTNIVYEWLKTLQLPQYAESFVDNGYDDLEVCKQIGDPDLDAIGVAVPQHRRRIHEAVRRLREADERAAGLYFTLEPPPAPPAKGPRRPPGQDGAAPRHRPGRGGPVVYPRLKLKIMIRDKLVRDGINLSKPPYSNKDGSLGNIDDLAQQYADYYNTCFTDVCERMEELRKRRVSQDLDVEKPDASPTSLQLRSQIEESLGLSSTASTPDTERKLSIHKSSSEEGSVDWKKKNKFFWQNFRKNQKGLMRQTSKGEDVGYVASEITMSDEERIQLMMMVKEKMITIEEALARLKEYESQHRQSSTVDATDWPDGSYSTFDGSSNCNSREQSDDETEESVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGVEEQSLDNSAILDDRSALYSGVHKKQFYFDGSSEKQTEDDSDSLTTSPSSSSLDTWGASRKLVKTFSKTDSRGLIKPPKKLGTFFSYPEDEKTQKVCRSLTDGEMKKSLGSLSHGRTCSFGGFDLTNRSLHIGNNSDQMGKEGDFVYKEVIKSPSASRISLGKKVKSVKETMKKRMSKKYSSSLSEQESSPGVIPGSPQSPPPDTDSLDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRRKSRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVEHLQEYDSNSDQSGSQEKLLIEGQGLAGCSPRDSGCYESSENLENGKTRRTCLLPSKSSSEHSFKDFSRHQLSNYPTLPLTKSIETLQQGEKESRLSCAHRALKSSVKTPAVMGLKKNRRSLPVAVCRSYETLDGPQSVDVWPRSHSLDDLQGDSDTNLQDGSKKDYFARDSLDTAKKATGSALPPQSCGGSCMVDNLMPKQGKAAAGSQKGRGKELECSVMESAVGKPFPFPMKHCEAQAALVTHSATRTPLEIQSKGFHDLARADYAPVLKGGLEAEQKGTNETRMQPKNPSQPPPVPAKKCRERLTNGLYHPPMTPSGNNSSQEAPCLPVKKTSASNPTDFHGVPVHRTSEQEPSTPPSSLPPWLLEQPETKSVQQHVIKLGPASARKVSCSRGMDLEMVIENKLQSEGIDLTEEPYSDKHGRCGIPEALVQRYSEDLDQPEKDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGLITSVSDWLISIGLPMYSSPLTEAGFNTLSKVPSLSQTCLQEAGITEERHISKLLAAARLFKPLDPDAI

Target ORF information:

RefSeq Version	XM_004935597.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004935597.2

ORF Insert Sequence:

ATGTGCACCA ACATAGTGTA CGAGTGGCTG AAAACCCTGC AGCTCCCCCA GTACGCCGAG 
TCCTTTGTCG ACAATGGCTA CGACGACCTG GAGGTCTGCA AGCAGATCGG CGACCCGGAC 
CTGGATGCCA TCGGCGTGGC CGTGCCCCAG CACCGCCGCC GCATCCACGA GGCCGTGCGG 
CGGCTGCGGG AGGCGGACGA GCGGGCGGCG GGGCTCTACT TCACCCTGGA GCCGCCGCCC 
GCGCCTCCCG CCAAGGGGCC CCGCCGCCCG CCGGGGCAGG ACGGGGCGGC CCCCCGGCAC 
CGGCCTGGCC GCGGGGGCCC GGTGGTCTAC CCCAGGCTGA AGCTGAAGAT CATGATCCGG 
GACAAGCTGG TCCGCGACGG CATCAACCTG AGCAAGCCCC CCTACTCCAA CAAGGATGGG 
TCTCTGGGAA ACATTGATGA CCTCGCACAG CAGTATGCAG ATTATTACAA CACCTGCTTC 
ACGGATGTGT GTGAGAGGAT GGAAGAGCTG CGCAAACGGA GGGTTTCCCA GGACCTTGAT 
GTGGAGAAAC CTGATGCCAG TCCCACATCT CTACAACTGC GATCCCAGAT TGAGGAGTCA 
CTTGGTCTCA GCAGCACCGC ATCAACACCT GACACTGAAA GAAAGCTCTC CATTCATAAA 
TCAAGTTCGG AAGAAGGCTC TGTAGACTGG AAAAAGAAAA ATAAGTTTTT TTGGCAAAAT 
TTCCGAAAGA ACCAGAAAGG ATTGATGAGG CAGACTTCAA AAGGAGAGGA TGTGGGGTAT 
GTGGCCAGTG AGATTACGAT GAGCGATGAG GAACGGATCC AGCTGATGAT GATGGTGAAA 
GAGAAGATGA TCACTATTGA GGAAGCACTT GCTAGGCTGA AGGAGTATGA GTCCCAGCAC 
AGGCAATCAA GTACTGTAGA TGCTACAGAC TGGCCTGATG GTTCTTACTC AACATTTGAT 
GGGTCTTCCA ATTGTAATTC AAGAGAACAA TCCGATGATG AAACAGAGGA GTCGGTGAAG 
TTTAAGAGAT TGCACAAGCT GGTAAATTCT ACTCGCAGAG TCAGGAAGAA ACTCATAAGG 
GTGGAAGAAA TGAAAAAACC CAGTACAGAA GGCGTAGAGG AGCAGTCGCT TGACAACTCT 
GCTATACTGG ATGACAGATC GGCACTTTAC TCTGGAGTTC ACAAGAAGCA ATTCTACTTT 
GATGGTTCCT CCGAGAAGCA GACAGAGGAT GACTCGGACT CACTTACTAC TTCTCCCTCA 
TCTAGTAGTC TTGATACATG GGGAGCTAGC CGGAAACTGG TGAAGACCTT CAGCAAAACT 
GATAGCCGTG GCCTTATCAA GCCTCCCAAG AAACTTGGGA CATTTTTTTC TTACCCAGAA 
GATGAGAAGA CCCAGAAAGT TTGCCGCTCC TTGACAGATG GGGAGATGAA GAAGAGCCTC 
GGCTCACTGA GCCATGGGAG AACCTGCAGC TTTGGAGGAT TTGACTTGAC AAATCGTTCC 
CTTCATATTG GAAACAATTC TGACCAAATG GGCAAAGAAG GTGACTTTGT TTATAAAGAA 
GTAATCAAAT CACCCTCTGC CTCCCGTATA TCTCTGGGCA AAAAGGTGAA ATCTGTGAAA 
GAAACCATGA AGAAAAGGAT GTCTAAAAAA TACAGCAGCT CTCTGTCTGA GCAGGAGTCG 
AGCCCTGGAG TCATACCGGG TTCTCCGCAG TCGCCGCCGC CAGACACTGA CTCTCTGGAC 
AAGCCCAAGC TGAAAGCTGG TGGCTCAGTT GAGAGCCTGA GGAGTTCCTT AAGTGGTCAG 
AGCTCAATGA GTGGTCAGAC AGTGAGTACA ACAGATTCCT CAACCAGCAA CAGGGAGAGC 
GTGAAATCAG AAGATGGGGA TGATGAAGAA CCTCCCTACA GAGGACCCTT CTGTGGAAGA 
GCCAGGGTTC ACACTGATTT TACTCCAAGT CCTTATGATA CAGACTCTCT GAAGCTCAAG 
AAAGGTGATA TCATTGACAT CATCAGCAAA CCCCCCATGG GCACTTGGAT GGGCCTATTG 
AACAACAAAG TTGGCACTTT CAAATTTATC TATGTGGATG TGTTAAATGA AGAGGAAGAG 
AAGCCGAAGC GACCCACCAG GAGACGCCGA AAAAGCAGAC CACCCCAACC CAAGTCAGTT 
GAGGATCTCT TGGATCGCAT CAACTTAAAG GAGCACATGC CCACTTTTCT GTTCAATGGT 
TATGAAGATC TGGATACCTT TAAGCTTTTA GAAGAAGAGG ATTTGGATGA ACTGAACATC 
CGAGATCCTG AGCACAGAGC TGTTCTCCTC ACAGCCGTGG AACATCTACA GGAGTATGAT 
AGTAACAGCG ACCAGTCAGG ATCTCAGGAG AAACTTCTCA TTGAAGGTCA AGGCCTAGCT 
GGATGCTCTC CTCGGGATTC TGGCTGCTAT GAAAGCAGTG AAAACCTGGA GAATGGTAAA 
ACCCGAAGGA CCTGTCTTCT GCCCTCAAAA TCATCAAGTG AGCATAGCTT TAAAGACTTC 
AGCAGACACC AGCTATCAAA TTATCCCACA CTCCCACTGA CCAAGTCAAT AGAAACTCTA 
CAGCAAGGAG AAAAAGAAAG CCGGTTGAGC TGTGCACATC GTGCTCTGAA GAGCTCTGTC 
AAGACCCCAG CTGTTATGGG TCTGAAGAAG AACCGTAGAA GTTTACCAGT TGCTGTCTGT 
CGGAGCTATG AAACTCTGGA TGGCCCCCAG AGTGTGGATG TATGGCCAAG ATCTCACTCC 
CTGGACGATC TCCAGGGAGA TTCCGATACT AATCTACAAG ATGGTAGCAA GAAAGATTAT 
TTTGCTCGGG ATTCACTGGA TACAGCGAAA AAGGCAACTG GATCTGCCCT GCCACCTCAG 
TCGTGTGGGG GCAGTTGTAT GGTCGATAAC TTGATGCCTA AGCAGGGTAA AGCAGCTGCT 
GGTTCTCAGA AGGGTAGAGG TAAGGAGTTG GAGTGCTCTG TAATGGAGAG TGCAGTTGGG 
AAACCTTTTC CATTCCCCAT GAAACACTGT GAAGCTCAGG CTGCCTTGGT GACACATTCT 
GCAACAAGGA CACCTCTGGA AATACAGAGT AAGGGCTTTC ATGACCTTGC ACGGGCTGAC 
TATGCTCCAG TCCTCAAGGG AGGTCTAGAA GCTGAGCAAA AAGGCACAAA TGAGACAAGA 
ATGCAACCAA AAAATCCCTC TCAGCCGCCA CCTGTTCCTG CCAAAAAATG CCGAGAACGC 
CTTACTAATG GATTATATCA TCCTCCCATG ACCCCCAGTG GGAACAACTC AAGTCAAGAA 
GCACCGTGTT TGCCAGTAAA AAAGACCAGT GCATCCAATC CCACTGATTT TCATGGAGTA 
CCTGTCCATA GGACATCTGA ACAGGAGCCC AGTACCCCTC CTAGCTCACT GCCACCCTGG 
CTGTTGGAGC AACCAGAGAC TAAAAGCGTT CAGCAGCACG TGATAAAGCT AGGGCCTGCT 
TCAGCAAGGA AAGTCTCTTG TAGCAGGGGA ATGGATCTGG AAATGGTAAT AGAAAACAAG 
TTGCAGTCTG AAGGTATTGA TCTTACTGAA GAACCATACT CAGACAAGCA TGGTCGCTGT 
GGCATTCCTG AGGCTTTGGT ACAGAGGTAC TCCGAAGACT TAGACCAGCC TGAAAAGGAT 
GTTGCTGCAA ACATGGACCA AATCCGGGTA AAGCAGCTGA GGAAGCAGCA TCGCATGGCA 
ATTCCAAGTG GAGGGCTCAC TGAAATTTGC CGAAAACCTG TATCACCAGG ACTTATCACC 
TCCGTATCTG ACTGGCTGAT TTCCATTGGC CTACCCATGT ATTCCAGCCC ACTCACAGAA 
GCAGGGTTCA ACACACTGAG CAAAGTGCCT TCTCTGTCAC AAACTTGCCT TCAGGAAGCT 
GGCATCACAG AGGAAAGGCA CATAAGCAAG CTCCTGGCAG CAGCAAGACT CTTCAAACCT 
CTCGATCCAG ACGCAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH1 ( XM_004935598.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004935655.1 Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa06283
Clone ID Related Accession (Same CDS sequence)	XM_004935598.2
Accession Version	XM_004935598.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	SAM and SH3 domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004935598.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACCA ACATAGTGTA CGAGTGGCTG AAAACCCTGC AGCTCCCCCA GTACGCCGAG 
TCCTTTGTCG ACAATGGCTA CGACGACCTG GAGGTCTGCA AGCAGATCGG CGACCCGGAC 
CTGGATGCCA TCGGCGTGGC CGTGCCCCAG CACCGCCGCC GCATCCACGA GGCCGTGCGG 
CGGCTGCGGG AGGCGGACGA GCGGGCGGCG GGGCTCTACT TCACCCTGGA GCCGCCGCCC 
GCGCCTCCCG CCAAGGGGCC CCGCCGCCCG CCGGGGCAGG ACGGGGCGGC CCCCCGGCAC 
CGGCCTGGCC GCGGGGGCCC GGTGGTCTAC CCCAGGCTGA AGCTGAAGAT CATGATCCGG 
GACAAGCTGG TCCGCGACGG CATCAACCTG AGCAAGCCCC CCTACTCCAA CAAGGATGGG 
TCTCTGGGAA ACATTGATGA CCTCGCACAG CAGTATGCAG ATTATTACAA CACCTGCTTC 
ACGGATGTGT GTGAGAGGAT GGAAGAGCTG CGCAAACGGA GGGTTTCCCA GGACCTTGAT 
GTGGAGAAAC CTGATGCCAG TCCCACATCT CTACAACTGC GATCCCAGAT TGAGGAGTCA 
CTTGGTCTCA GCAGCACCGC ATCAACACCT GACACTGAAA GAAAGCTCTC CATTCATAAA 
TCAAGTTCGG AAGAAGGCTC TGTAGGGAAA GCAGACTGGA AAAAGAAAAA TAAGTTTTTT 
TGGCAAAATT TCCGAAAGAA CCAGAAAGGA TTGATGAGGC AGACTTCAAA AGGAGAGGAT 
GTGGGGTATG TGGCCAGTGA GATTACGATG AGCGATGAGG AACGGATCCA GCTGATGATG 
ATGGTGAAAG AGAAGATGAT CACTATTGAG GAAGCACTTG CTAGGTCAAG AGAACAATCC 
GATGATGAAA CAGAGGAGTC GGTGAAGTTT AAGAGATTGC ACAAGCTGGT AAATTCTACT 
CGCAGAGTCA GGAAGAAACT CATAAGGGTG GAAGAAATGA AAAAACCCAG TACAGAAGGC 
GTAGAGGAGC AGTCGCTTGA CAACTCTGCT ATACTGGATG ACAGATCGGC ACTTTACTCT 
GGAGTTCACA AGAAGCAATT CTACTTTGAT GGTTCCTCCG AGAAGCAGAC AGAGGATGAC 
TCGGACTCAC TTACTACTTC TCCCTCATCT AGTAGTCTTG ATACATGGGG AGCTAGCCGG 
AAACTGGTGA AGACCTTCAG CAAAACTGAT AGCCGTGGCC TTATCAAGCC TCCCAAGAAA 
CTTGGGACAT TTTTTTCTTA CCCAGAAGAT GAGAAGACCC AGAAAGTTTG CCGCTCCTTG 
ACAGATGGGG AGATGAAGAA GAGCCTCGGC TCACTGAGCC ATGGGAGAAC CTGCAGCTTT 
GGAGGATTTG ACTTGACAAA TCGTTCCCTT CATATTGGAA ACAATTCTGA CCAAATGGGC 
AAAGAAGGTG ACTTTGTTTA TAAAGAAGTA ATCAAATCAC CCTCTGCCTC CCGTATATCT 
CTGGGCAAAA AGGTGAAATC TGTGAAAGAA ACCATGAAGA AAAGGATGTC TAAAAAATAC 
AGCAGCTCTC TGTCTGAGCA GGAGTCGAGC CCTGGAGTCA TACCGGGTTC TCCGCAGTCG 
CCGCCGCCAG ACACTGACTC TCTGGACAAG CCCAAGCTGA AAGCTGGTGG CTCAGTTGAG 
AGCCTGAGGA GTTCCTTAAG TGGTCAGAGC TCAATGAGTG GTCAGACAGT GAGTACAACA 
GATTCCTCAA CCAGCAACAG GGAGAGCGTG AAATCAGAAG ATGGGGATGA TGAAGAACCT 
CCCTACAGAG GACCCTTCTG TGGAAGAGCC AGGGTTCACA CTGATTTTAC TCCAAGTCCT 
TATGATACAG ACTCTCTGAA GCTCAAGAAA GGTGATATCA TTGACATCAT CAGCAAACCC 
CCCATGGGCA CTTGGATGGG CCTATTGAAC AACAAAGTTG GCACTTTCAA ATTTATCTAT 
GTGGATGTGT TAAATGAAGA GGAAGAGAAG CCGAAGCGAC CCACCAGGAG ACGCCGAAAA 
AGCAGACCAC CCCAACCCAA GTCAGTTGAG GATCTCTTGG ATCGCATCAA CTTAAAGGAG 
CACATGCCCA CTTTTCTGTT CAATGGTTAT GAAGATCTGG ATACCTTTAA GCTTTTAGAA 
GAAGAGGATT TGGATGAACT GAACATCCGA GATCCTGAGC ACAGAGCTGT TCTCCTCACA 
GCCGTGGAAC ATCTACAGGA GTATGATAGT AACAGCGACC AGTCAGGATC TCAGGAGAAA 
CTTCTCATTG AAGGTCAAGG CCTAGCTGGA TGCTCTCCTC GGGATTCTGG CTGCTATGAA 
AGCAGTGAAA ACCTGGAGAA TGGTAAAACC CGAAGGACCT GTCTTCTGCC CTCAAAATCA 
TCAAGTGAGC ATAGCTTTAA AGACTTCAGC AGACACCAGC TATCAAATTA TCCCACACTC 
CCACTGACCA AGTCAATAGA AACTCTACAG CAAGGAGAAA AAGAAAGCCG GTTGAGCTGT 
GCACATCGTG CTCTGAAGAG CTCTGTCAAG ACCCCAGCTG TTATGGGTCT GAAGAAGAAC 
CGTAGAAGTT TACCAGTTGC TGTCTGTCGG AGCTATGAAA CTCTGGATGG CCCCCAGAGT 
GTGGATGTAT GGCCAAGATC TCACTCCCTG GACGATCTCC AGGGAGATTC CGATACTAAT 
CTACAAGATG GTAGCAAGAA AGATTATTTT GCTCGGGATT CACTGGATAC AGCGAAAAAG 
GCAACTGGAT CTGCCCTGCC ACCTCAGTCG TGTGGGGGCA GTTGTATGGT CGATAACTTG 
ATGCCTAAGC AGGGTAAAGC AGCTGCTGGT TCTCAGAAGG GTAGAGGTAA GGAGTTGGAG 
TGCTCTGTAA TGGAGAGTGC AGTTGGGAAA CCTTTTCCAT TCCCCATGAA ACACTGTGAA 
GCTCAGGCTG CCTTGGTGAC ACATTCTGCA ACAAGGACAC CTCTGGAAAT ACAGAGTAAG 
GGCTTTCATG ACCTTGCACG GGCTGACTAT GCTCCAGTCC TCAAGGGAGG TCTAGAAGCT 
GAGCAAAAAG GCACAAATGA GACAAGAATG CAACCAAAAA ATCCCTCTCA GCCGCCACCT 
GTTCCTGCCA AAAAATGCCG AGAACGCCTT ACTAATGGAT TATATCATCC TCCCATGACC 
CCCAGTGGGA ACAACTCAAG TCAAGAAGCA CCGTGTTTGC CAGTAAAAAA GACCAGTGCA 
TCCAATCCCA CTGATTTTCA TGGAGTACCT GTCCATAGGA CATCTGAACA GGAGCCCAGT 
ACCCCTCCTA GCTCACTGCC ACCCTGGCTG TTGGAGCAAC CAGAGACTAA AAGCGTTCAG 
CAGCACGTGA TAAAGCTAGG GCCTGCTTCA GCAAGGAAAG TCTCTTGTAG CAGGGGAATG 
GATCTGGAAA TGGTAATAGA AAACAAGTTG CAGTCTGAAG GTATTGATCT TACTGAAGAA 
CCATACTCAG ACAAGCATGG TCGCTGTGGC ATTCCTGAGG CTTTGGTACA GAGGTACTCC 
GAAGACTTAG ACCAGCCTGA AAAGGATGTT GCTGCAAACA TGGACCAAAT CCGGGTAAAG 
CAGCTGAGGA AGCAGCATCG CATGGCAATT CCAAGTGGAG GGCTCACTGA AATTTGCCGA 
AAACCTGTAT CACCAGGACT TATCACCTCC GTATCTGACT GGCTGATTTC CATTGGCCTA 
CCCATGTATT CCAGCCCACT CACAGAAGCA GGGTTCAACA CACTGAGCAA AGTGCCTTCT 
CTGTCACAAA CTTGCCTTCA GGAAGCTGGC ATCACAGAGG AAAGGCACAT AAGCAAGCTC 
CTGGCAGCAG CAAGACTCTT CAAACCTCTC GATCCAGACG CAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004935655.1
CDS	728..4615
Translation	MCTNIVYEWLKTLQLPQYAESFVDNGYDDLEVCKQIGDPDLDAIGVAVPQHRRRIHEAVRRLREADERAAGLYFTLEPPPAPPAKGPRRPPGQDGAAPRHRPGRGGPVVYPRLKLKIMIRDKLVRDGINLSKPPYSNKDGSLGNIDDLAQQYADYYNTCFTDVCERMEELRKRRVSQDLDVEKPDASPTSLQLRSQIEESLGLSSTASTPDTERKLSIHKSSSEEGSVGKADWKKKNKFFWQNFRKNQKGLMRQTSKGEDVGYVASEITMSDEERIQLMMMVKEKMITIEEALARSREQSDDETEESVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGVEEQSLDNSAILDDRSALYSGVHKKQFYFDGSSEKQTEDDSDSLTTSPSSSSLDTWGASRKLVKTFSKTDSRGLIKPPKKLGTFFSYPEDEKTQKVCRSLTDGEMKKSLGSLSHGRTCSFGGFDLTNRSLHIGNNSDQMGKEGDFVYKEVIKSPSASRISLGKKVKSVKETMKKRMSKKYSSSLSEQESSPGVIPGSPQSPPPDTDSLDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRRKSRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVEHLQEYDSNSDQSGSQEKLLIEGQGLAGCSPRDSGCYESSENLENGKTRRTCLLPSKSSSEHSFKDFSRHQLSNYPTLPLTKSIETLQQGEKESRLSCAHRALKSSVKTPAVMGLKKNRRSLPVAVCRSYETLDGPQSVDVWPRSHSLDDLQGDSDTNLQDGSKKDYFARDSLDTAKKATGSALPPQSCGGSCMVDNLMPKQGKAAAGSQKGRGKELECSVMESAVGKPFPFPMKHCEAQAALVTHSATRTPLEIQSKGFHDLARADYAPVLKGGLEAEQKGTNETRMQPKNPSQPPPVPAKKCRERLTNGLYHPPMTPSGNNSSQEAPCLPVKKTSASNPTDFHGVPVHRTSEQEPSTPPSSLPPWLLEQPETKSVQQHVIKLGPASARKVSCSRGMDLEMVIENKLQSEGIDLTEEPYSDKHGRCGIPEALVQRYSEDLDQPEKDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGLITSVSDWLISIGLPMYSSPLTEAGFNTLSKVPSLSQTCLQEAGITEERHISKLLAAARLFKPLDPDAI

Target ORF information:

RefSeq Version	XM_004935598.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAM and SH3 domain containing 1 (SASH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004935598.2

ORF Insert Sequence:

ATGTGCACCA ACATAGTGTA CGAGTGGCTG AAAACCCTGC AGCTCCCCCA GTACGCCGAG 
TCCTTTGTCG ACAATGGCTA CGACGACCTG GAGGTCTGCA AGCAGATCGG CGACCCGGAC 
CTGGATGCCA TCGGCGTGGC CGTGCCCCAG CACCGCCGCC GCATCCACGA GGCCGTGCGG 
CGGCTGCGGG AGGCGGACGA GCGGGCGGCG GGGCTCTACT TCACCCTGGA GCCGCCGCCC 
GCGCCTCCCG CCAAGGGGCC CCGCCGCCCG CCGGGGCAGG ACGGGGCGGC CCCCCGGCAC 
CGGCCTGGCC GCGGGGGCCC GGTGGTCTAC CCCAGGCTGA AGCTGAAGAT CATGATCCGG 
GACAAGCTGG TCCGCGACGG CATCAACCTG AGCAAGCCCC CCTACTCCAA CAAGGATGGG 
TCTCTGGGAA ACATTGATGA CCTCGCACAG CAGTATGCAG ATTATTACAA CACCTGCTTC 
ACGGATGTGT GTGAGAGGAT GGAAGAGCTG CGCAAACGGA GGGTTTCCCA GGACCTTGAT 
GTGGAGAAAC CTGATGCCAG TCCCACATCT CTACAACTGC GATCCCAGAT TGAGGAGTCA 
CTTGGTCTCA GCAGCACCGC ATCAACACCT GACACTGAAA GAAAGCTCTC CATTCATAAA 
TCAAGTTCGG AAGAAGGCTC TGTAGGGAAA GCAGACTGGA AAAAGAAAAA TAAGTTTTTT 
TGGCAAAATT TCCGAAAGAA CCAGAAAGGA TTGATGAGGC AGACTTCAAA AGGAGAGGAT 
GTGGGGTATG TGGCCAGTGA GATTACGATG AGCGATGAGG AACGGATCCA GCTGATGATG 
ATGGTGAAAG AGAAGATGAT CACTATTGAG GAAGCACTTG CTAGGTCAAG AGAACAATCC 
GATGATGAAA CAGAGGAGTC GGTGAAGTTT AAGAGATTGC ACAAGCTGGT AAATTCTACT 
CGCAGAGTCA GGAAGAAACT CATAAGGGTG GAAGAAATGA AAAAACCCAG TACAGAAGGC 
GTAGAGGAGC AGTCGCTTGA CAACTCTGCT ATACTGGATG ACAGATCGGC ACTTTACTCT 
GGAGTTCACA AGAAGCAATT CTACTTTGAT GGTTCCTCCG AGAAGCAGAC AGAGGATGAC 
TCGGACTCAC TTACTACTTC TCCCTCATCT AGTAGTCTTG ATACATGGGG AGCTAGCCGG 
AAACTGGTGA AGACCTTCAG CAAAACTGAT AGCCGTGGCC TTATCAAGCC TCCCAAGAAA 
CTTGGGACAT TTTTTTCTTA CCCAGAAGAT GAGAAGACCC AGAAAGTTTG CCGCTCCTTG 
ACAGATGGGG AGATGAAGAA GAGCCTCGGC TCACTGAGCC ATGGGAGAAC CTGCAGCTTT 
GGAGGATTTG ACTTGACAAA TCGTTCCCTT CATATTGGAA ACAATTCTGA CCAAATGGGC 
AAAGAAGGTG ACTTTGTTTA TAAAGAAGTA ATCAAATCAC CCTCTGCCTC CCGTATATCT 
CTGGGCAAAA AGGTGAAATC TGTGAAAGAA ACCATGAAGA AAAGGATGTC TAAAAAATAC 
AGCAGCTCTC TGTCTGAGCA GGAGTCGAGC CCTGGAGTCA TACCGGGTTC TCCGCAGTCG 
CCGCCGCCAG ACACTGACTC TCTGGACAAG CCCAAGCTGA AAGCTGGTGG CTCAGTTGAG 
AGCCTGAGGA GTTCCTTAAG TGGTCAGAGC TCAATGAGTG GTCAGACAGT GAGTACAACA 
GATTCCTCAA CCAGCAACAG GGAGAGCGTG AAATCAGAAG ATGGGGATGA TGAAGAACCT 
CCCTACAGAG GACCCTTCTG TGGAAGAGCC AGGGTTCACA CTGATTTTAC TCCAAGTCCT 
TATGATACAG ACTCTCTGAA GCTCAAGAAA GGTGATATCA TTGACATCAT CAGCAAACCC 
CCCATGGGCA CTTGGATGGG CCTATTGAAC AACAAAGTTG GCACTTTCAA ATTTATCTAT 
GTGGATGTGT TAAATGAAGA GGAAGAGAAG CCGAAGCGAC CCACCAGGAG ACGCCGAAAA 
AGCAGACCAC CCCAACCCAA GTCAGTTGAG GATCTCTTGG ATCGCATCAA CTTAAAGGAG 
CACATGCCCA CTTTTCTGTT CAATGGTTAT GAAGATCTGG ATACCTTTAA GCTTTTAGAA 
GAAGAGGATT TGGATGAACT GAACATCCGA GATCCTGAGC ACAGAGCTGT TCTCCTCACA 
GCCGTGGAAC ATCTACAGGA GTATGATAGT AACAGCGACC AGTCAGGATC TCAGGAGAAA 
CTTCTCATTG AAGGTCAAGG CCTAGCTGGA TGCTCTCCTC GGGATTCTGG CTGCTATGAA 
AGCAGTGAAA ACCTGGAGAA TGGTAAAACC CGAAGGACCT GTCTTCTGCC CTCAAAATCA 
TCAAGTGAGC ATAGCTTTAA AGACTTCAGC AGACACCAGC TATCAAATTA TCCCACACTC 
CCACTGACCA AGTCAATAGA AACTCTACAG CAAGGAGAAA AAGAAAGCCG GTTGAGCTGT 
GCACATCGTG CTCTGAAGAG CTCTGTCAAG ACCCCAGCTG TTATGGGTCT GAAGAAGAAC 
CGTAGAAGTT TACCAGTTGC TGTCTGTCGG AGCTATGAAA CTCTGGATGG CCCCCAGAGT 
GTGGATGTAT GGCCAAGATC TCACTCCCTG GACGATCTCC AGGGAGATTC CGATACTAAT 
CTACAAGATG GTAGCAAGAA AGATTATTTT GCTCGGGATT CACTGGATAC AGCGAAAAAG 
GCAACTGGAT CTGCCCTGCC ACCTCAGTCG TGTGGGGGCA GTTGTATGGT CGATAACTTG 
ATGCCTAAGC AGGGTAAAGC AGCTGCTGGT TCTCAGAAGG GTAGAGGTAA GGAGTTGGAG 
TGCTCTGTAA TGGAGAGTGC AGTTGGGAAA CCTTTTCCAT TCCCCATGAA ACACTGTGAA 
GCTCAGGCTG CCTTGGTGAC ACATTCTGCA ACAAGGACAC CTCTGGAAAT ACAGAGTAAG 
GGCTTTCATG ACCTTGCACG GGCTGACTAT GCTCCAGTCC TCAAGGGAGG TCTAGAAGCT 
GAGCAAAAAG GCACAAATGA GACAAGAATG CAACCAAAAA ATCCCTCTCA GCCGCCACCT 
GTTCCTGCCA AAAAATGCCG AGAACGCCTT ACTAATGGAT TATATCATCC TCCCATGACC 
CCCAGTGGGA ACAACTCAAG TCAAGAAGCA CCGTGTTTGC CAGTAAAAAA GACCAGTGCA 
TCCAATCCCA CTGATTTTCA TGGAGTACCT GTCCATAGGA CATCTGAACA GGAGCCCAGT 
ACCCCTCCTA GCTCACTGCC ACCCTGGCTG TTGGAGCAAC CAGAGACTAA AAGCGTTCAG 
CAGCACGTGA TAAAGCTAGG GCCTGCTTCA GCAAGGAAAG TCTCTTGTAG CAGGGGAATG 
GATCTGGAAA TGGTAATAGA AAACAAGTTG CAGTCTGAAG GTATTGATCT TACTGAAGAA 
CCATACTCAG ACAAGCATGG TCGCTGTGGC ATTCCTGAGG CTTTGGTACA GAGGTACTCC 
GAAGACTTAG ACCAGCCTGA AAAGGATGTT GCTGCAAACA TGGACCAAAT CCGGGTAAAG 
CAGCTGAGGA AGCAGCATCG CATGGCAATT CCAAGTGGAG GGCTCACTGA AATTTGCCGA 
AAACCTGTAT CACCAGGACT TATCACCTCC GTATCTGACT GGCTGATTTC CATTGGCCTA 
CCCATGTATT CCAGCCCACT CACAGAAGCA GGGTTCAACA CACTGAGCAA AGTGCCTTCT 
CTGTCACAAA CTTGCCTTCA GGAAGCTGGC ATCACAGAGG AAAGGCACAT AAGCAAGCTC 
CTGGCAGCAG CAAGACTCTT CAAACCTCTC GATCCAGACG CAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SASH1
Entrez Gene ID	421621
Full Name	SAM and SH3 domain containing 1
General protein information	Preferred Names SAM and SH3 domain containing 1 Names SAM and SH3 domain-containing protein 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Canis lupus familiaris (dog)	SASH1	XP_850394.1
Bos taurus (cattle)	SASH1	XP_002690361.1
Danio rerio (zebrafish)	sash1a	NP_001038284.1
Homo sapiens (human)	SASH1	NP_056093.3
	SASH1	XP_002803910.1
Gallus gallus (chicken)	SASH1	XP_004935653.1
Pan troglodytes (chimpanzee)	SASH1	XP_518791.3
Mus musculus (house mouse)	Sash1	NP_780364.3
Rattus norvegicus (Norway rat)	Sash1	XP_003748693.1
Xenopus tropicalis (tropical clawed frog)	sash1	XP_002936309.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SASH1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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