SRGAP2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SRGAP2
Entrez Gene ID	556198
Full Name	SLIT-ROBO Rho GTPase activating protein 2
Synonyms	srgap2a,wu:fb47h12,zgc:136814
General protein information	Preferred Names SLIT-ROBO Rho GTPase activating protein 2 Names SLIT-ROBO Rho GTPase-activating protein 2 SLIT-ROBO Rho GTPase activating protein 2a
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017358230.1	XP_017213719.1	SLIT-ROBO Rho GTPase-activating protein 2 isoform X4
XM_005172876.4	XP_005172933.1	SLIT-ROBO Rho GTPase-activating protein 2 isoform X1
XM_009305994.2	XP_009304269.1	SLIT-ROBO Rho GTPase-activating protein 2 isoform X1
NM_001048235.1	NP_001041700.1	SLIT-ROBO Rho GTPase-activating protein 2
XM_009305995.2	XP_009304270.1	SLIT-ROBO Rho GTPase-activating protein 2 isoform X2
XM_005172876.3	XP_005172933.1	SLIT-ROBO Rho GTPase-activating protein 2 isoform X3

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SRGAP2	XP_417972.3
Homo sapiens (human)	SRGAP2	NP_056141.2
	SRGAP2	XP_001089021.1
Pan troglodytes (chimpanzee)	SRGAP2	XP_001163967.1
Canis lupus familiaris (dog)	SRGAP2	XP_005640863.1
Mus musculus (house mouse)	Srgap2	NP_001074480.2
Danio rerio (zebrafish)	srgap2a	NP_001041700.1
Xenopus tropicalis (tropical clawed frog)	srgap2	XP_002940554.1
Bos taurus (cattle)	SRGAP2	NP_001192794.1
Rattus norvegicus (Norway rat)	Srgap2	NP_001128430.1

Gene Ontology
Bibliography

Related articles in PubMed

Dissection of Glomerular Transcriptional Profile in Patients with Diabetic Nephropathy: SRGAP2a Protects Podocyte Structure and Function.
Pan Y, Jiang S, Hou Q, Qiu D, Shi J, Wang L, Chen Z, Zhang M, Duan A, Qin W, Zen K, Liu Z
Diabetes(2017 Dec)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SRGAP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SRGAP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa54268	XM_017358230.1 Latest version!	Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
ODa38107	NM_001048235.1 Latest version!	Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa17034	XM_005172876.4 Latest version!	Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa42319	XM_009305994.2 Latest version!	Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa42320	XM_009305995.2 Latest version!	Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa17034	XM_005172876.3	Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SRGAP2 ( XM_017358230.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213719.1 Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa54268
Clone ID Related Accession (Same CDS sequence)	XM_017358230.1
Accession Version	XM_017358230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SLIT-ROBO Rho GTPase-activating protein 2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCTC CTGCTAAATT CCGAAAGGAC AAGGAAATTA TAGCCGAATA TGAAACGCAA 
GTTAAAGAGA TCCGAGCTCA GTTGGTGGAG CAGCTTAAGT GTTTGGACCA GCAATGTGAG 
CTCCGTGTGC AGCTCCTGCA AGACCTGCAG GACTTCTTCC GCAAGAAGGC AGAGATTGAG 
ATGGACTACT CCCGCAACCT AGAGAAACTA GCTGAGAGGT TCCTGGCAAA GACACGCTAC 
ACAAAAGACC CTCAATTCAA AAAAGAGCAG AATATCTTGT CTCCAGTCAA CTGCTGGAAC 
CTTCTCTTGG CTCAGGTGAA GAGAGAAAGT CGAGACCATG CCACTCTCAG TGATCTCTAC 
CTCAACAACA TCATCCCACG CTTCGCCCAG ATCAGCGAAG ACTCAGGACG CCTCTTTAAA 
AAGAGCAAAG AGGTGGGCCT CCAGCTGCAG GAGGATCTGA TGAAAGTCCT CAATGAGTTG 
TACACGGTGA TGAAGACTTA TCACATGTAC AACATGGACA GCATCAACGC AGAGAGCAAG 
CTGAAGGAGG CAGAGAAGCA GGAGGAGAAA CAGATGAGCC GCAGTGTCCG GCATGAGGAA 
AAACAGACCC CTCGCTCACC TGACTCTCTC ACCAACATCA AGATCGAGGA TAAACACGTG 
CGCCGCAGCT CCGTCAAGAA GATTGAGAAG ATGAAGGAGA AGCGGCAGGC CAAATACACT 
GAGAATAAGC TGAAAGCTAT CAAGGCCAGG AATGAGTATC TTCTGGCTCT GGAGGCCACA 
AACAGCTGCG TCTTCAAATA CTACATCCAT GATCTGTCTG ACCTCATCGA TTGCTGTGAT 
CTGGGCTACC ATGCCAGCCT GAACCGTGCA CTGCGTACCT ACCTCTCGGC TGAGTTTAAC 
GTGGAGACAT CCAAACACGG GGGCCTGGAG ACCATTGAGA ATGCAGCAGA AAACCTGGAG 
GCCAACTCAG ACAAACAGCG GCTCATGGAG ACCTACAACA ACGTCTTCTG TCCTCCGATG 
CGCTTCGATT TCCAGTCTCA CATGGGTGAC ATGGTTGGAC ATTTGTGTGC CCAGCAGCCT 
GTGCAGGGAG AGCTGATCCA GAGATGCCAG CAGCTTCAGT CCCGTCTGTC CACACTGAAT 
ATAGAGAATG AAGAGGTTAA GAAGACCATG GAGGCCACTT TACAGACCAT CCAGGACATG 
GTGACCATAG AGGACTTCGA TGTGACCGAC TGCTTCCACC ACAGCAACTC AATGGAGTCG 
GTCAAGTCCA CAGTGTCTGA GTCTTTCATG AGCAAACCCA GCCTGGCCAA GAGACGAGCC 
AACCAGCAGG AGACCGAACA GTTCTACTTC ACGGTAAATG AGACTGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213719.1
CDS	477..1847
Translation	MTSPAKFRKDKEIIAEYETQVKEIRAQLVEQLKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKLAERFLAKTRYTKDPQFKKEQNILSPVNCWNLLLAQVKRESRDHATLSDLYLNNIIPRFAQISEDSGRLFKKSKEVGLQLQEDLMKVLNELYTVMKTYHMYNMDSINAESKLKEAEKQEEKQMSRSVRHEEKQTPRSPDSLTNIKIEDKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNSCVFKYYIHDLSDLIDCCDLGYHASLNRALRTYLSAEFNVETSKHGGLETIENAAENLEANSDKQRLMETYNNVFCPPMRFDFQSHMGDMVGHLCAQQPVQGELIQRCQQLQSRLSTLNIENEEVKKTMEATLQTIQDMVTIEDFDVTDCFHHSNSMESVKSTVSESFMSKPSLAKRRANQQETEQFYFTVNETE

Target ORF information:

RefSeq Version	XM_017358230.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358230.1

ORF Insert Sequence:

ATGACGTCTC CTGCTAAATT CCGAAAGGAC AAGGAAATTA TAGCCGAATA TGAAACGCAA 
GTTAAAGAGA TCCGAGCTCA GTTGGTGGAG CAGCTTAAGT GTTTGGACCA GCAATGTGAG 
CTCCGTGTGC AGCTCCTGCA AGACCTGCAG GACTTCTTCC GCAAGAAGGC AGAGATTGAG 
ATGGACTACT CCCGCAACCT AGAGAAACTA GCTGAGAGGT TCCTGGCAAA GACACGCTAC 
ACAAAAGACC CTCAATTCAA AAAAGAGCAG AATATCTTGT CTCCAGTCAA CTGCTGGAAC 
CTTCTCTTGG CTCAGGTGAA GAGAGAAAGT CGAGACCATG CCACTCTCAG TGATCTCTAC 
CTCAACAACA TCATCCCACG CTTCGCCCAG ATCAGCGAAG ACTCAGGACG CCTCTTTAAA 
AAGAGCAAAG AGGTGGGCCT CCAGCTGCAG GAGGATCTGA TGAAAGTCCT CAATGAGTTG 
TACACGGTGA TGAAGACTTA TCACATGTAC AACATGGACA GCATCAACGC AGAGAGCAAG 
CTGAAGGAGG CAGAGAAGCA GGAGGAGAAA CAGATGAGCC GCAGTGTCCG GCATGAGGAA 
AAACAGACCC CTCGCTCACC TGACTCTCTC ACCAACATCA AGATCGAGGA TAAACACGTG 
CGCCGCAGCT CCGTCAAGAA GATTGAGAAG ATGAAGGAGA AGCGGCAGGC CAAATACACT 
GAGAATAAGC TGAAAGCTAT CAAGGCCAGG AATGAGTATC TTCTGGCTCT GGAGGCCACA 
AACAGCTGCG TCTTCAAATA CTACATCCAT GATCTGTCTG ACCTCATCGA TTGCTGTGAT 
CTGGGCTACC ATGCCAGCCT GAACCGTGCA CTGCGTACCT ACCTCTCGGC TGAGTTTAAC 
GTGGAGACAT CCAAACACGG GGGCCTGGAG ACCATTGAGA ATGCAGCAGA AAACCTGGAG 
GCCAACTCAG ACAAACAGCG GCTCATGGAG ACCTACAACA ACGTCTTCTG TCCTCCGATG 
CGCTTCGATT TCCAGTCTCA CATGGGTGAC ATGGTTGGAC ATTTGTGTGC CCAGCAGCCT 
GTGCAGGGAG AGCTGATCCA GAGATGCCAG CAGCTTCAGT CCCGTCTGTC CACACTGAAT 
ATAGAGAATG AAGAGGTTAA GAAGACCATG GAGGCCACTT TACAGACCAT CCAGGACATG 
GTGACCATAG AGGACTTCGA TGTGACCGAC TGCTTCCACC ACAGCAACTC AATGGAGTCG 
GTCAAGTCCA CAGTGTCTGA GTCTTTCATG AGCAAACCCA GCCTGGCCAA GAGACGAGCC 
AACCAGCAGG AGACCGAACA GTTCTACTTC ACGGTAAATG AGACTGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRGAP2 ( NM_001048235.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001041700.1 Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), mRNA.

Price & Availability↑

CloneID	ODa38107
Clone ID Related Accession (Same CDS sequence)	NM_001048235.1
Accession Version	NM_001048235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	SLIT-ROBO Rho GTPase-activating protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC122886.1. On Sep 27, 2006 this sequence version replaced XM_678951.1. ##Evidence-Data-START## Transcript exon combination :: BC122886.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACGTCTC CTGCTAAATT CCGAAAGGAC AAGGAAATTA TAGCCGAATA TGAAACGCAA 
GTTAAAGAGA TCCGAGCTCA GTTGGTGGAG CAGCTTAAGT GTTTGGACCA GCAATGTGAG 
CTCCGTGTGC AGCTCCTGCA AGACCTGCAG GACTTCTTCC GCAAGAAGGC AGAGATTGAG 
ATGGACTACT CCCGCAACCT AGAGAAACTA GCTGAGAGGT TCCTGGCAAA GACACGCTAC 
ACAAAAGACC CTCAATTCAA AAAAGAGCAG AATATCTTGT CTCCAGTCAA CTGCTGGAAC 
CTTCTCTTGG CTCAGGTGAA GAGAGAAAGT CGAGACCATG CCACTCTCAG TGATCTCTAC 
CTCAACAACA TCATCCCACG CTTCGCCCAG ATCAGCGAAG ACTCAGGACG CCTCTTTAAA 
AAGAGCAAAG AGGTGGGCCT CCAGCTGCAG GAGGATCTGA TGAAAGTCCT CAATGAGTTG 
TACACGGTGA TGAAGACCTA TCACATGTAC AACATGGACA GCATCAACGC AGAGAGCAAG 
CTGAAGGAGG CAGAGAAGCA GGAGGAGAAA CAGATGAGCC GCAGTGTCCG GCATGAGGAA 
AAACAGACCC CTCGCTCACC TGACTCTCTC ACCAACATCA AGATCGAGGA TAAACACGTG 
CGCCGCAGCT CCGTCAAGAA GATTGAGAAG ATGAAGGAGA AGCGGCAGGC CAAATACACT 
GAGAATAAGC TGAAAGCTAT CAAGGCCAGG AATGAGTATC TTCTGGCTCT GGAGGCCACA 
AACAGCTGCG TCTTCAAATA CTACATCCAT GATCTGTCTG ACCTCATCGA TTGCTGTGAT 
CTGGGCTACC ATGCCAGCCT GAACCGTGCA CTGCGTACCT ACCTCTCGGC TGAGTTTAAC 
GTGGAGACAT CCAAACACGG GGGCCTGGAG ACCATTGAGA ATGCAGCAAA AAACCTGGAG 
GCCAACTCAG ACAAACAGCG GCTCATGGAG ACCTACAACA ACGTCTTCTG TCCTCCGATG 
CGCTTCGATT TCCAGTCTCA CATGGGTGAC ATGGTTGGAC ATTTGTGTGC CCAGCAGCCT 
GTGCAGGGAG AGCTGATCCA GAGATGCCAG CAGCTTCAGT CCCGTCTGTC CACACTGAAT 
ATAGAGAATG AAGAGGTTAA GAAGACCATG GAGGCCACTT TACAGACCAT CCAGGACATG 
GTGACCATAG AGGACTTCGA TGTGACCGAC TGCTTCCACC ACAGCAACTC AATGGAGTCG 
GTCAAGTCCA CAGTGTCTGA GTCTTTCATG AGCAAACCCA GCCTGGCCAA GAGACGAGCC 
AACCAGCAGG AGACCGAACA GTTCTACTTC ACGAAACTGA AGGAGTTTCT GGAGGGCAGG 
AACCTCATCA CCAAACTGCA GGCTAAACAC GACCTGATCC AAAAGACTCT GGGAGAAAGT 
CAGAAGACGG ACTACTGCCT TGCCAGCGGA CGGAGGGACT CCACTGTGCG GAAACAGGAA 
GCCATCCAAA TCATTCCCCT GATGGTGGAG AGTTGTATTC GCTTCATCAG TCGACACGGT 
CTTCATCATG AGGGGATTTT CAGGGTGTCT GGCTCCCAGG TGGAGGTCAA TGACATTAAA 
AACGCCTTTG AAAGAGGTGA GGATCCGCTG GCTGGAGATC AGAATGATCA CGATATGGAC 
TCCATCGCTG GAGTGCTGAA GCTCTACTTC AGAGGCCTGG AAAACGCCCT GTTTCCTAAG 
GAAGTCTTCC ATGACCTCAT GTCCTGTGTC TCCATTGAGA GCCTCCAGGA AAGAGCTGTG 
CACATCCGAA AAGTCCTGCT GTCGCTGCCC AGCAACATCC TGGTTATCAT GAGATACCTG 
TTTGCTTTTC TCAATCATCT GTCTCAGTAC AGTGACGACA ACATGATGGA CCCCTACAAC 
CTGGCTATCT GCTTCGGCCC CACACTCATG TCTGTTCCTG AGGGCCACGA TCAGGTCTCC 
TGCCAGGCAC ATGTGAACGA GCTCATCAAA ACCATTATTA TCCACCACGA GAGCATCTTT 
CCAGGGCCCC GTGAGCTGGA GGGCCCCGTC TACGACCGTG GAGGAGCCCC TGAGGAATAC 
TGTGATAGTC CACACATTGA GCCCCCTCTA GTGGACGAAC CTGCTCCTGA CACGGTCTCT 
GTAATCCACA ACAGTGATGA CGTAAAGTCA GGGCCATTGA CCGTTTCAGA GTCGGACCCC 
ATCGAGGCCA TCGCTCGCTT CGATTACTCT GGACGCACCA ACCGCGAGCT GAGCTTCAAA 
AAGGGGGCGT CTCTGCTGCT GTACAGCCGC GCATCTGATG ACTGGTGGGA GGGACGACAC 
AACGGCACTG AGGGTCTCGT GCCGCACCAG TACATAGTAG TGCAGGACAT GCCGGATGGA 
TATGCAGGCA GAGGAAGTCC TAAAGCTGAC CTCGAAGGAT CCCATGATTC AGTGGAAGAG 
AAGGTTTCCA CGAGAGCCAG TGCCAGCTCA CCCACTGGAG GTCATGTGGC TGATATCTAT 
CTAGCCAACC TCAACAAGTT GAGGAAGCGT CCAGAAGCGA CGAGCATCCG GAGAACCATC 
CGGCCTGTGG AGGAGGGCTC ATCAGGAGCA GCAGGGGGCT TAAAGACCTC CTCTATGCCT 
GCTGGAGGAC TGGCCAAGGA CAGCTCAGAC AAGCGTCCTG TCAGCGCTCA CAGCGTCCTG 
AACTCCATCA CCAGACACTC GTCTCTCAAG ACTAAAGTAG AGGGTCCTCA AGTTCGCAAA 
TCCACACCTA CTGGACGATC CAAGAGCTTC AGCAACCACA GACCCCTCGA CCCAGAGGTG 
ATCGCGCAGG TGGAGCACAG CTCACAGGAC ATCGAGGCCA CTATGAACAC GGCTCTGAGT 
GAGCTGCGAG AGCTTGAGAG GCAGAGCAAC GTCAAACATG CTCCAGACGT GGTTCTCGAC 
ACACTGGAGC AGCTCAAAAG TGGCGGGACA TCTGAACCAT CCAGCCCTCT CCATTCCCGC 
CTGCTCCGCG AAGCTGAATC CTCTCAGCAT CCTCTGCAGC GCAGCGCCAG TTCAGCCAGC 
GACATGCCCT CCACTTTCCG ACCCAGCAAA ACCACAGGCC CCAAGAGCCC TCTGTCCTCC 
ATGACCACCG CTTCTGGCTC CACATTCAGA GATAACAAAC CTCCAGCCAC CAGACCCAAG 
CCTGTGGTGT TCCCCAAAAG CAGTTCAGCC GGAGGAAGTC CGGCTATGGG ATCTCCAACC 
ACCACCATCC CACCAACACC ACCACCTCCA CCACCACCCA CCGACAAATC CTGCCCCGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001041700.1
CDS	407..3709
Translation	MTSPAKFRKDKEIIAEYETQVKEIRAQLVEQLKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKLAERFLAKTRYTKDPQFKKEQNILSPVNCWNLLLAQVKRESRDHATLSDLYLNNIIPRFAQISEDSGRLFKKSKEVGLQLQEDLMKVLNELYTVMKTYHMYNMDSINAESKLKEAEKQEEKQMSRSVRHEEKQTPRSPDSLTNIKIEDKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNSCVFKYYIHDLSDLIDCCDLGYHASLNRALRTYLSAEFNVETSKHGGLETIENAAKNLEANSDKQRLMETYNNVFCPPMRFDFQSHMGDMVGHLCAQQPVQGELIQRCQQLQSRLSTLNIENEEVKKTMEATLQTIQDMVTIEDFDVTDCFHHSNSMESVKSTVSESFMSKPSLAKRRANQQETEQFYFTKLKEFLEGRNLITKLQAKHDLIQKTLGESQKTDYCLASGRRDSTVRKQEAIQIIPLMVESCIRFISRHGLHHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLENALFPKEVFHDLMSCVSIESLQERAVHIRKVLLSLPSNILVIMRYLFAFLNHLSQYSDDNMMDPYNLAICFGPTLMSVPEGHDQVSCQAHVNELIKTIIIHHESIFPGPRELEGPVYDRGGAPEEYCDSPHIEPPLVDEPAPDTVSVIHNSDDVKSGPLTVSESDPIEAIARFDYSGRTNRELSFKKGASLLLYSRASDDWWEGRHNGTEGLVPHQYIVVQDMPDGYAGRGSPKADLEGSHDSVEEKVSTRASASSPTGGHVADIYLANLNKLRKRPEATSIRRTIRPVEEGSSGAAGGLKTSSMPAGGLAKDSSDKRPVSAHSVLNSITRHSSLKTKVEGPQVRKSTPTGRSKSFSNHRPLDPEVIAQVEHSSQDIEATMNTALSELRELERQSNVKHAPDVVLDTLEQLKSGGTSEPSSPLHSRLLREAESSQHPLQRSASSASDMPSTFRPSKTTGPKSPLSSMTTASGSTFRDNKPPATRPKPVVFPKSSSAGGSPAMGSPTTTIPPTPPPPPPPTDKSCPV

Target ORF information:

RefSeq Version	NM_001048235.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001048235.1

ORF Insert Sequence:

ATGACGTCTC CTGCTAAATT CCGAAAGGAC AAGGAAATTA TAGCCGAATA TGAAACGCAA 
GTTAAAGAGA TCCGAGCTCA GTTGGTGGAG CAGCTTAAGT GTTTGGACCA GCAATGTGAG 
CTCCGTGTGC AGCTCCTGCA AGACCTGCAG GACTTCTTCC GCAAGAAGGC AGAGATTGAG 
ATGGACTACT CCCGCAACCT AGAGAAACTA GCTGAGAGGT TCCTGGCAAA GACACGCTAC 
ACAAAAGACC CTCAATTCAA AAAAGAGCAG AATATCTTGT CTCCAGTCAA CTGCTGGAAC 
CTTCTCTTGG CTCAGGTGAA GAGAGAAAGT CGAGACCATG CCACTCTCAG TGATCTCTAC 
CTCAACAACA TCATCCCACG CTTCGCCCAG ATCAGCGAAG ACTCAGGACG CCTCTTTAAA 
AAGAGCAAAG AGGTGGGCCT CCAGCTGCAG GAGGATCTGA TGAAAGTCCT CAATGAGTTG 
TACACGGTGA TGAAGACCTA TCACATGTAC AACATGGACA GCATCAACGC AGAGAGCAAG 
CTGAAGGAGG CAGAGAAGCA GGAGGAGAAA CAGATGAGCC GCAGTGTCCG GCATGAGGAA 
AAACAGACCC CTCGCTCACC TGACTCTCTC ACCAACATCA AGATCGAGGA TAAACACGTG 
CGCCGCAGCT CCGTCAAGAA GATTGAGAAG ATGAAGGAGA AGCGGCAGGC CAAATACACT 
GAGAATAAGC TGAAAGCTAT CAAGGCCAGG AATGAGTATC TTCTGGCTCT GGAGGCCACA 
AACAGCTGCG TCTTCAAATA CTACATCCAT GATCTGTCTG ACCTCATCGA TTGCTGTGAT 
CTGGGCTACC ATGCCAGCCT GAACCGTGCA CTGCGTACCT ACCTCTCGGC TGAGTTTAAC 
GTGGAGACAT CCAAACACGG GGGCCTGGAG ACCATTGAGA ATGCAGCAAA AAACCTGGAG 
GCCAACTCAG ACAAACAGCG GCTCATGGAG ACCTACAACA ACGTCTTCTG TCCTCCGATG 
CGCTTCGATT TCCAGTCTCA CATGGGTGAC ATGGTTGGAC ATTTGTGTGC CCAGCAGCCT 
GTGCAGGGAG AGCTGATCCA GAGATGCCAG CAGCTTCAGT CCCGTCTGTC CACACTGAAT 
ATAGAGAATG AAGAGGTTAA GAAGACCATG GAGGCCACTT TACAGACCAT CCAGGACATG 
GTGACCATAG AGGACTTCGA TGTGACCGAC TGCTTCCACC ACAGCAACTC AATGGAGTCG 
GTCAAGTCCA CAGTGTCTGA GTCTTTCATG AGCAAACCCA GCCTGGCCAA GAGACGAGCC 
AACCAGCAGG AGACCGAACA GTTCTACTTC ACGAAACTGA AGGAGTTTCT GGAGGGCAGG 
AACCTCATCA CCAAACTGCA GGCTAAACAC GACCTGATCC AAAAGACTCT GGGAGAAAGT 
CAGAAGACGG ACTACTGCCT TGCCAGCGGA CGGAGGGACT CCACTGTGCG GAAACAGGAA 
GCCATCCAAA TCATTCCCCT GATGGTGGAG AGTTGTATTC GCTTCATCAG TCGACACGGT 
CTTCATCATG AGGGGATTTT CAGGGTGTCT GGCTCCCAGG TGGAGGTCAA TGACATTAAA 
AACGCCTTTG AAAGAGGTGA GGATCCGCTG GCTGGAGATC AGAATGATCA CGATATGGAC 
TCCATCGCTG GAGTGCTGAA GCTCTACTTC AGAGGCCTGG AAAACGCCCT GTTTCCTAAG 
GAAGTCTTCC ATGACCTCAT GTCCTGTGTC TCCATTGAGA GCCTCCAGGA AAGAGCTGTG 
CACATCCGAA AAGTCCTGCT GTCGCTGCCC AGCAACATCC TGGTTATCAT GAGATACCTG 
TTTGCTTTTC TCAATCATCT GTCTCAGTAC AGTGACGACA ACATGATGGA CCCCTACAAC 
CTGGCTATCT GCTTCGGCCC CACACTCATG TCTGTTCCTG AGGGCCACGA TCAGGTCTCC 
TGCCAGGCAC ATGTGAACGA GCTCATCAAA ACCATTATTA TCCACCACGA GAGCATCTTT 
CCAGGGCCCC GTGAGCTGGA GGGCCCCGTC TACGACCGTG GAGGAGCCCC TGAGGAATAC 
TGTGATAGTC CACACATTGA GCCCCCTCTA GTGGACGAAC CTGCTCCTGA CACGGTCTCT 
GTAATCCACA ACAGTGATGA CGTAAAGTCA GGGCCATTGA CCGTTTCAGA GTCGGACCCC 
ATCGAGGCCA TCGCTCGCTT CGATTACTCT GGACGCACCA ACCGCGAGCT GAGCTTCAAA 
AAGGGGGCGT CTCTGCTGCT GTACAGCCGC GCATCTGATG ACTGGTGGGA GGGACGACAC 
AACGGCACTG AGGGTCTCGT GCCGCACCAG TACATAGTAG TGCAGGACAT GCCGGATGGA 
TATGCAGGCA GAGGAAGTCC TAAAGCTGAC CTCGAAGGAT CCCATGATTC AGTGGAAGAG 
AAGGTTTCCA CGAGAGCCAG TGCCAGCTCA CCCACTGGAG GTCATGTGGC TGATATCTAT 
CTAGCCAACC TCAACAAGTT GAGGAAGCGT CCAGAAGCGA CGAGCATCCG GAGAACCATC 
CGGCCTGTGG AGGAGGGCTC ATCAGGAGCA GCAGGGGGCT TAAAGACCTC CTCTATGCCT 
GCTGGAGGAC TGGCCAAGGA CAGCTCAGAC AAGCGTCCTG TCAGCGCTCA CAGCGTCCTG 
AACTCCATCA CCAGACACTC GTCTCTCAAG ACTAAAGTAG AGGGTCCTCA AGTTCGCAAA 
TCCACACCTA CTGGACGATC CAAGAGCTTC AGCAACCACA GACCCCTCGA CCCAGAGGTG 
ATCGCGCAGG TGGAGCACAG CTCACAGGAC ATCGAGGCCA CTATGAACAC GGCTCTGAGT 
GAGCTGCGAG AGCTTGAGAG GCAGAGCAAC GTCAAACATG CTCCAGACGT GGTTCTCGAC 
ACACTGGAGC AGCTCAAAAG TGGCGGGACA TCTGAACCAT CCAGCCCTCT CCATTCCCGC 
CTGCTCCGCG AAGCTGAATC CTCTCAGCAT CCTCTGCAGC GCAGCGCCAG TTCAGCCAGC 
GACATGCCCT CCACTTTCCG ACCCAGCAAA ACCACAGGCC CCAAGAGCCC TCTGTCCTCC 
ATGACCACCG CTTCTGGCTC CACATTCAGA GATAACAAAC CTCCAGCCAC CAGACCCAAG 
CCTGTGGTGT TCCCCAAAAG CAGTTCAGCC GGAGGAAGTC CGGCTATGGG ATCTCCAACC 
ACCACCATCC CACCAACACC ACCACCTCCA CCACCACCCA CCGACAAATC CTGCCCCGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRGAP2 ( XM_005172876.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005172933.1 Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17034
Clone ID Related Accession (Same CDS sequence)	XM_005172876.4 , XM_005172876.3
Accession Version	XM_005172876.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SLIT-ROBO Rho GTPase-activating protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172876.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCTC CTGCTAAATT CCGAAAGGAC AAGGAAATTA TAGCCGAATA TGAAACGCAA 
GTTAAAGAGA TCCGAGCTCA GTTGGTGGAG CAGCTTAAGT GTTTGGACCA GCAATGTGAG 
CTCCGTGTGC AGCTCCTGCA AGACCTGCAG GACTTCTTCC GCAAGAAGGC AGAGATTGAG 
ATGGACTACT CCCGCAACCT AGAGAAACTA GCTGAGAGGT TCCTGGCAAA GACACGCTAC 
ACAAAAGACC CTCAATTCAA AAAAGAGCAG AATATCTTGT CTCCAGTCAA CTGCTGGAAC 
CTTCTCTTGG CTCAGGTGAA GAGAGAAAGT CGAGACCATG CCACTCTCAG TGATCTCTAC 
CTCAACAACA TCATCCCACG CTTCGCCCAG ATCAGCGAAG ACTCAGGACG CCTCTTTAAA 
AAGAGCAAAG AGGTGGGCCT CCAGCTGCAG GAGGATCTGA TGAAAGTCCT CAATGAGTTG 
TACACGGTGA TGAAGACTTA TCACATGTAC AACATGGACA GCATCAACGC AGAGAGCAAG 
CTGAAGGAGG CAGAGAAGCA GGAGGAGAAA CAGATGAGCC GCAGTGTCCG GCATGAGGAA 
AAACAGACCC CTCGCTCACC TGACTCTCTC ACCAACATCA AGATCGAGGA TAAACACGTG 
CGCCGCAGCT CCGTCAAGAA GATTGAGAAG ATGAAGGAGA AGCGGCAGGC CAAATACACT 
GAGAATAAGC TGAAAGCTAT CAAGGCCAGG AATGAGTATC TTCTGGCTCT GGAGGCCACA 
AACAGCTGCG TCTTCAAATA CTACATCCAT GATCTGTCTG ACCTCATCGA TTGCTGTGAT 
CTGGGCTACC ATGCCAGCCT GAACCGTGCA CTGCGTACCT ACCTCTCGGC TGAGTTTAAC 
GTGGAGACAT CCAAACACGG GGGCCTGGAG ACCATTGAGA ATGCAGCAGA AAACCTGGAG 
GCCAACTCAG ACAAACAGCG GCTCATGGAG ACCTACAACA ACGTCTTCTG TCCTCCGATG 
CGCTTCGATT TCCAGTCTCA CATGGGTGAC ATGGTTGGAC ATTTGTGTGC CCAGCAGCCT 
GTGCAGGGAG AGCTGATCCA GAGATGCCAG CAGCTTCAGT CCCGTCTGTC CACACTGAAT 
ATAGAGAATG AAGAGGTTAA GAAGACCATG GAGGCCACTT TACAGACCAT CCAGGACATG 
GTGACCATAG AGGACTTCGA TGTGACCGAC TGCTTCCACC ACAGCAACTC AATGGAGTCG 
GTCAAGTCCA CAGTGTCTGA GTCTTTCATG AGCAAACCCA GCCTGGCCAA GAGACGAGCC 
AACCAGCAGG AGACCGAACA GTTCTACTTC ACGAAACTGA AGGAGTTTCT GGAGGGCAGG 
AACCTCATCA CCAAACTGCA GGCTAAACAC GATCTGATCC AAAAGACTCT GGGAGAAAGT 
CAGAAGACGG ACTACTGCCT TGCCAGCGGA CGGAGGGACT CCACTGTGCG GAAACAGGAG 
GCCATCCAAA TCATTCCCCT GATGGTGGAG AGTTGTATTC GCTTCATCAG TCGACACGGT 
CTCCATCATG AGGGGATTTT CAGGGTGTCT GGCTCCCAGG TGGAGGTCAA TGACATTAAA 
AACGCCTTTG AAAGAGGTGA GGATCCGCTG GCTGGAGATC AGAATGATCA CGATATGGAC 
TCCATCGCTG GAGTGCTGAA GCTCTACTTC AGAGGCCTGG AAAACGCCCT GTTTCCTAAG 
GAAGTCTTCC ATGACCTCAT GTCCTGTGTC TCCATTGAGA GCCTCCAGGA AAGAGCTGTG 
CACATCCGAA AAGTCCTGCT GTCGCTGCCC AGCAACATCC TGGTTATCAT GAGATACCTG 
TTTGCTTTTC TCAATCATCT GTCTCAGTAC AGTGACGACA ACATGATGGA CCCCTACAAC 
CTGGCTATCT GCTTCGGCCC CACACTCATG TCTGTTCCTG AGGGCCACGA TCAAGTCTCC 
TGCCAGGCAC ATGTGAACGA GCTCATCAAA ACCATTATTA TCCACCACGA GAGCATCTTT 
CCAGGGCCCC GTGAGCTGGA GGGCCCCGTC TACGACCGTG GAGGAGCCCC TGAGGAATAC 
TGTGATAGTC CACACATTGA GCCCCCTCTA GTGGACGAAC CTGCTCCTGA CACGGTCTCT 
GTAATCCACA ACAGTGATGA CGAGTCGGAC CCCATCGAGG CCATCGCTCG CTTCGATTAC 
TCTGGACGCA CCAACCGCGA GCTGAGCTTC AAAAAGGGGG CGTCTCTGCT GCTGTACAGC 
CGCGCATCTG ATGACTGGTG GGAGGGACGA CACAACGGCA CTGAGGGTCT CGTGCCGCAC 
CAGTACATAG TAGTGCAGGA CATGCCGGAT GGATATGCAG GCAGAGGAAG TCCTAAAGCT 
GACCTCGAAG GATCCCATGA TTCAGTGGAA GAGAAGGTTT CCACGAGAGC CAGTGCCAGC 
TCACCCACTG GAGGTCATGT GGCTGATATC TATCTAGCCA ACCTCAACAA GTTGAGGAAG 
CGTCCAGAAG CGACGAGCAT CCGGAGAACC ATCCGGCCTG TGGAGGAGGG CTCATCAGGA 
GCAGCAGGGG GCTTAAAGAC CTCCTCTATG CCTGCTGGAG GACTGGCCAA GGACAGCTCA 
GACAAGCGTC CTGTCAGCGC TCACAGCGTC CTGAACTCCA TCACCAGACA CTCGTCTCTC 
AAGACTAAAG TAGAGGGTCC TCAAGTTCGC AAATCCACAC CTACTGGACG ATCCAAGAGC 
TTCAGCAACC ACAGACCCCT CGACCCAGAG GTGATCGCGC AGGTGGAGCA CAGCTCACAG 
GACATCGAGG CCACTATGAA CACGGCTCTG AGTGAGCTGC GAGAGCTTGA GAGGCAGAGC 
AACGTCAAAC ATGCTCCAGA CGTGGTTCTC GACACACTGG AGCAGCTCAA AAGTGGCGGG 
ACATCTGAAC CATCCAGCCC TCTCCATTCC CGCCTGCTCC GCGAAGCTGA ATCCTCTCAG 
CATCCTCTGC AGCGCAGCGC CAGTTCAGCC AGCGACATGC CCTCCACTTT CCGACCCAGC 
AAAACCACAG GCCCCAAGAG CCCTCTGTCC TCCATGACCA CCGCTTCTGG CTCCACATTC 
AGAGATAACA AACCTCCAGC CACCAGACCC AAGCCTGTGG TGTTCCCCAA AAGCAGTTCA 
GCCGGAGGAA GTCCGGCTAT GGGATCTCCA ACCACCACCA TCCCACCAAC ACCACCACCT 
CCACCACCAC CCACCGACAA ATCCTGCCCC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172933.1
CDS	483..3758
Translation	MTSPAKFRKDKEIIAEYETQVKEIRAQLVEQLKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKLAERFLAKTRYTKDPQFKKEQNILSPVNCWNLLLAQVKRESRDHATLSDLYLNNIIPRFAQISEDSGRLFKKSKEVGLQLQEDLMKVLNELYTVMKTYHMYNMDSINAESKLKEAEKQEEKQMSRSVRHEEKQTPRSPDSLTNIKIEDKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNSCVFKYYIHDLSDLIDCCDLGYHASLNRALRTYLSAEFNVETSKHGGLETIENAAENLEANSDKQRLMETYNNVFCPPMRFDFQSHMGDMVGHLCAQQPVQGELIQRCQQLQSRLSTLNIENEEVKKTMEATLQTIQDMVTIEDFDVTDCFHHSNSMESVKSTVSESFMSKPSLAKRRANQQETEQFYFTKLKEFLEGRNLITKLQAKHDLIQKTLGESQKTDYCLASGRRDSTVRKQEAIQIIPLMVESCIRFISRHGLHHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLENALFPKEVFHDLMSCVSIESLQERAVHIRKVLLSLPSNILVIMRYLFAFLNHLSQYSDDNMMDPYNLAICFGPTLMSVPEGHDQVSCQAHVNELIKTIIIHHESIFPGPRELEGPVYDRGGAPEEYCDSPHIEPPLVDEPAPDTVSVIHNSDDESDPIEAIARFDYSGRTNRELSFKKGASLLLYSRASDDWWEGRHNGTEGLVPHQYIVVQDMPDGYAGRGSPKADLEGSHDSVEEKVSTRASASSPTGGHVADIYLANLNKLRKRPEATSIRRTIRPVEEGSSGAAGGLKTSSMPAGGLAKDSSDKRPVSAHSVLNSITRHSSLKTKVEGPQVRKSTPTGRSKSFSNHRPLDPEVIAQVEHSSQDIEATMNTALSELRELERQSNVKHAPDVVLDTLEQLKSGGTSEPSSPLHSRLLREAESSQHPLQRSASSASDMPSTFRPSKTTGPKSPLSSMTTASGSTFRDNKPPATRPKPVVFPKSSSAGGSPAMGSPTTTIPPTPPPPPPPTDKSCPV

Target ORF information:

RefSeq Version	XM_005172876.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172876.4

ORF Insert Sequence:

ATGACGTCTC CTGCTAAATT CCGAAAGGAC AAGGAAATTA TAGCCGAATA TGAAACGCAA 
GTTAAAGAGA TCCGAGCTCA GTTGGTGGAG CAGCTTAAGT GTTTGGACCA GCAATGTGAG 
CTCCGTGTGC AGCTCCTGCA AGACCTGCAG GACTTCTTCC GCAAGAAGGC AGAGATTGAG 
ATGGACTACT CCCGCAACCT AGAGAAACTA GCTGAGAGGT TCCTGGCAAA GACACGCTAC 
ACAAAAGACC CTCAATTCAA AAAAGAGCAG AATATCTTGT CTCCAGTCAA CTGCTGGAAC 
CTTCTCTTGG CTCAGGTGAA GAGAGAAAGT CGAGACCATG CCACTCTCAG TGATCTCTAC 
CTCAACAACA TCATCCCACG CTTCGCCCAG ATCAGCGAAG ACTCAGGACG CCTCTTTAAA 
AAGAGCAAAG AGGTGGGCCT CCAGCTGCAG GAGGATCTGA TGAAAGTCCT CAATGAGTTG 
TACACGGTGA TGAAGACTTA TCACATGTAC AACATGGACA GCATCAACGC AGAGAGCAAG 
CTGAAGGAGG CAGAGAAGCA GGAGGAGAAA CAGATGAGCC GCAGTGTCCG GCATGAGGAA 
AAACAGACCC CTCGCTCACC TGACTCTCTC ACCAACATCA AGATCGAGGA TAAACACGTG 
CGCCGCAGCT CCGTCAAGAA GATTGAGAAG ATGAAGGAGA AGCGGCAGGC CAAATACACT 
GAGAATAAGC TGAAAGCTAT CAAGGCCAGG AATGAGTATC TTCTGGCTCT GGAGGCCACA 
AACAGCTGCG TCTTCAAATA CTACATCCAT GATCTGTCTG ACCTCATCGA TTGCTGTGAT 
CTGGGCTACC ATGCCAGCCT GAACCGTGCA CTGCGTACCT ACCTCTCGGC TGAGTTTAAC 
GTGGAGACAT CCAAACACGG GGGCCTGGAG ACCATTGAGA ATGCAGCAGA AAACCTGGAG 
GCCAACTCAG ACAAACAGCG GCTCATGGAG ACCTACAACA ACGTCTTCTG TCCTCCGATG 
CGCTTCGATT TCCAGTCTCA CATGGGTGAC ATGGTTGGAC ATTTGTGTGC CCAGCAGCCT 
GTGCAGGGAG AGCTGATCCA GAGATGCCAG CAGCTTCAGT CCCGTCTGTC CACACTGAAT 
ATAGAGAATG AAGAGGTTAA GAAGACCATG GAGGCCACTT TACAGACCAT CCAGGACATG 
GTGACCATAG AGGACTTCGA TGTGACCGAC TGCTTCCACC ACAGCAACTC AATGGAGTCG 
GTCAAGTCCA CAGTGTCTGA GTCTTTCATG AGCAAACCCA GCCTGGCCAA GAGACGAGCC 
AACCAGCAGG AGACCGAACA GTTCTACTTC ACGAAACTGA AGGAGTTTCT GGAGGGCAGG 
AACCTCATCA CCAAACTGCA GGCTAAACAC GATCTGATCC AAAAGACTCT GGGAGAAAGT 
CAGAAGACGG ACTACTGCCT TGCCAGCGGA CGGAGGGACT CCACTGTGCG GAAACAGGAG 
GCCATCCAAA TCATTCCCCT GATGGTGGAG AGTTGTATTC GCTTCATCAG TCGACACGGT 
CTCCATCATG AGGGGATTTT CAGGGTGTCT GGCTCCCAGG TGGAGGTCAA TGACATTAAA 
AACGCCTTTG AAAGAGGTGA GGATCCGCTG GCTGGAGATC AGAATGATCA CGATATGGAC 
TCCATCGCTG GAGTGCTGAA GCTCTACTTC AGAGGCCTGG AAAACGCCCT GTTTCCTAAG 
GAAGTCTTCC ATGACCTCAT GTCCTGTGTC TCCATTGAGA GCCTCCAGGA AAGAGCTGTG 
CACATCCGAA AAGTCCTGCT GTCGCTGCCC AGCAACATCC TGGTTATCAT GAGATACCTG 
TTTGCTTTTC TCAATCATCT GTCTCAGTAC AGTGACGACA ACATGATGGA CCCCTACAAC 
CTGGCTATCT GCTTCGGCCC CACACTCATG TCTGTTCCTG AGGGCCACGA TCAAGTCTCC 
TGCCAGGCAC ATGTGAACGA GCTCATCAAA ACCATTATTA TCCACCACGA GAGCATCTTT 
CCAGGGCCCC GTGAGCTGGA GGGCCCCGTC TACGACCGTG GAGGAGCCCC TGAGGAATAC 
TGTGATAGTC CACACATTGA GCCCCCTCTA GTGGACGAAC CTGCTCCTGA CACGGTCTCT 
GTAATCCACA ACAGTGATGA CGAGTCGGAC CCCATCGAGG CCATCGCTCG CTTCGATTAC 
TCTGGACGCA CCAACCGCGA GCTGAGCTTC AAAAAGGGGG CGTCTCTGCT GCTGTACAGC 
CGCGCATCTG ATGACTGGTG GGAGGGACGA CACAACGGCA CTGAGGGTCT CGTGCCGCAC 
CAGTACATAG TAGTGCAGGA CATGCCGGAT GGATATGCAG GCAGAGGAAG TCCTAAAGCT 
GACCTCGAAG GATCCCATGA TTCAGTGGAA GAGAAGGTTT CCACGAGAGC CAGTGCCAGC 
TCACCCACTG GAGGTCATGT GGCTGATATC TATCTAGCCA ACCTCAACAA GTTGAGGAAG 
CGTCCAGAAG CGACGAGCAT CCGGAGAACC ATCCGGCCTG TGGAGGAGGG CTCATCAGGA 
GCAGCAGGGG GCTTAAAGAC CTCCTCTATG CCTGCTGGAG GACTGGCCAA GGACAGCTCA 
GACAAGCGTC CTGTCAGCGC TCACAGCGTC CTGAACTCCA TCACCAGACA CTCGTCTCTC 
AAGACTAAAG TAGAGGGTCC TCAAGTTCGC AAATCCACAC CTACTGGACG ATCCAAGAGC 
TTCAGCAACC ACAGACCCCT CGACCCAGAG GTGATCGCGC AGGTGGAGCA CAGCTCACAG 
GACATCGAGG CCACTATGAA CACGGCTCTG AGTGAGCTGC GAGAGCTTGA GAGGCAGAGC 
AACGTCAAAC ATGCTCCAGA CGTGGTTCTC GACACACTGG AGCAGCTCAA AAGTGGCGGG 
ACATCTGAAC CATCCAGCCC TCTCCATTCC CGCCTGCTCC GCGAAGCTGA ATCCTCTCAG 
CATCCTCTGC AGCGCAGCGC CAGTTCAGCC AGCGACATGC CCTCCACTTT CCGACCCAGC 
AAAACCACAG GCCCCAAGAG CCCTCTGTCC TCCATGACCA CCGCTTCTGG CTCCACATTC 
AGAGATAACA AACCTCCAGC CACCAGACCC AAGCCTGTGG TGTTCCCCAA AAGCAGTTCA 
GCCGGAGGAA GTCCGGCTAT GGGATCTCCA ACCACCACCA TCCCACCAAC ACCACCACCT 
CCACCACCAC CCACCGACAA ATCCTGCCCC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRGAP2 ( XM_005172876.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005172933.1 Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa17034
Clone ID Related Accession (Same CDS sequence)	XM_005172876.4 , XM_005172876.3
Accession Version	XM_005172876.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SLIT-ROBO Rho GTPase-activating protein 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172876.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCTC CTGCTAAATT CCGAAAGGAC AAGGAAATTA TAGCCGAATA TGAAACGCAA 
GTTAAAGAGA TCCGAGCTCA GTTGGTGGAG CAGCTTAAGT GTTTGGACCA GCAATGTGAG 
CTCCGTGTGC AGCTCCTGCA AGACCTGCAG GACTTCTTCC GCAAGAAGGC AGAGATTGAG 
ATGGACTACT CCCGCAACCT AGAGAAACTA GCTGAGAGGT TCCTGGCAAA GACACGCTAC 
ACAAAAGACC CTCAATTCAA AAAAGAGCAG AATATCTTGT CTCCAGTCAA CTGCTGGAAC 
CTTCTCTTGG CTCAGGTGAA GAGAGAAAGT CGAGACCATG CCACTCTCAG TGATCTCTAC 
CTCAACAACA TCATCCCACG CTTCGCCCAG ATCAGCGAAG ACTCAGGACG CCTCTTTAAA 
AAGAGCAAAG AGGTGGGCCT CCAGCTGCAG GAGGATCTGA TGAAAGTCCT CAATGAGTTG 
TACACGGTGA TGAAGACTTA TCACATGTAC AACATGGACA GCATCAACGC AGAGAGCAAG 
CTGAAGGAGG CAGAGAAGCA GGAGGAGAAA CAGATGAGCC GCAGTGTCCG GCATGAGGAA 
AAACAGACCC CTCGCTCACC TGACTCTCTC ACCAACATCA AGATCGAGGA TAAACACGTG 
CGCCGCAGCT CCGTCAAGAA GATTGAGAAG ATGAAGGAGA AGCGGCAGGC CAAATACACT 
GAGAATAAGC TGAAAGCTAT CAAGGCCAGG AATGAGTATC TTCTGGCTCT GGAGGCCACA 
AACAGCTGCG TCTTCAAATA CTACATCCAT GATCTGTCTG ACCTCATCGA TTGCTGTGAT 
CTGGGCTACC ATGCCAGCCT GAACCGTGCA CTGCGTACCT ACCTCTCGGC TGAGTTTAAC 
GTGGAGACAT CCAAACACGG GGGCCTGGAG ACCATTGAGA ATGCAGCAGA AAACCTGGAG 
GCCAACTCAG ACAAACAGCG GCTCATGGAG ACCTACAACA ACGTCTTCTG TCCTCCGATG 
CGCTTCGATT TCCAGTCTCA CATGGGTGAC ATGGTTGGAC ATTTGTGTGC CCAGCAGCCT 
GTGCAGGGAG AGCTGATCCA GAGATGCCAG CAGCTTCAGT CCCGTCTGTC CACACTGAAT 
ATAGAGAATG AAGAGGTTAA GAAGACCATG GAGGCCACTT TACAGACCAT CCAGGACATG 
GTGACCATAG AGGACTTCGA TGTGACCGAC TGCTTCCACC ACAGCAACTC AATGGAGTCG 
GTCAAGTCCA CAGTGTCTGA GTCTTTCATG AGCAAACCCA GCCTGGCCAA GAGACGAGCC 
AACCAGCAGG AGACCGAACA GTTCTACTTC ACGAAACTGA AGGAGTTTCT GGAGGGCAGG 
AACCTCATCA CCAAACTGCA GGCTAAACAC GATCTGATCC AAAAGACTCT GGGAGAAAGT 
CAGAAGACGG ACTACTGCCT TGCCAGCGGA CGGAGGGACT CCACTGTGCG GAAACAGGAG 
GCCATCCAAA TCATTCCCCT GATGGTGGAG AGTTGTATTC GCTTCATCAG TCGACACGGT 
CTCCATCATG AGGGGATTTT CAGGGTGTCT GGCTCCCAGG TGGAGGTCAA TGACATTAAA 
AACGCCTTTG AAAGAGGTGA GGATCCGCTG GCTGGAGATC AGAATGATCA CGATATGGAC 
TCCATCGCTG GAGTGCTGAA GCTCTACTTC AGAGGCCTGG AAAACGCCCT GTTTCCTAAG 
GAAGTCTTCC ATGACCTCAT GTCCTGTGTC TCCATTGAGA GCCTCCAGGA AAGAGCTGTG 
CACATCCGAA AAGTCCTGCT GTCGCTGCCC AGCAACATCC TGGTTATCAT GAGATACCTG 
TTTGCTTTTC TCAATCATCT GTCTCAGTAC AGTGACGACA ACATGATGGA CCCCTACAAC 
CTGGCTATCT GCTTCGGCCC CACACTCATG TCTGTTCCTG AGGGCCACGA TCAAGTCTCC 
TGCCAGGCAC ATGTGAACGA GCTCATCAAA ACCATTATTA TCCACCACGA GAGCATCTTT 
CCAGGGCCCC GTGAGCTGGA GGGCCCCGTC TACGACCGTG GAGGAGCCCC TGAGGAATAC 
TGTGATAGTC CACACATTGA GCCCCCTCTA GTGGACGAAC CTGCTCCTGA CACGGTCTCT 
GTAATCCACA ACAGTGATGA CGAGTCGGAC CCCATCGAGG CCATCGCTCG CTTCGATTAC 
TCTGGACGCA CCAACCGCGA GCTGAGCTTC AAAAAGGGGG CGTCTCTGCT GCTGTACAGC 
CGCGCATCTG ATGACTGGTG GGAGGGACGA CACAACGGCA CTGAGGGTCT CGTGCCGCAC 
CAGTACATAG TAGTGCAGGA CATGCCGGAT GGATATGCAG GCAGAGGAAG TCCTAAAGCT 
GACCTCGAAG GATCCCATGA TTCAGTGGAA GAGAAGGTTT CCACGAGAGC CAGTGCCAGC 
TCACCCACTG GAGGTCATGT GGCTGATATC TATCTAGCCA ACCTCAACAA GTTGAGGAAG 
CGTCCAGAAG CGACGAGCAT CCGGAGAACC ATCCGGCCTG TGGAGGAGGG CTCATCAGGA 
GCAGCAGGGG GCTTAAAGAC CTCCTCTATG CCTGCTGGAG GACTGGCCAA GGACAGCTCA 
GACAAGCGTC CTGTCAGCGC TCACAGCGTC CTGAACTCCA TCACCAGACA CTCGTCTCTC 
AAGACTAAAG TAGAGGGTCC TCAAGTTCGC AAATCCACAC CTACTGGACG ATCCAAGAGC 
TTCAGCAACC ACAGACCCCT CGACCCAGAG GTGATCGCGC AGGTGGAGCA CAGCTCACAG 
GACATCGAGG CCACTATGAA CACGGCTCTG AGTGAGCTGC GAGAGCTTGA GAGGCAGAGC 
AACGTCAAAC ATGCTCCAGA CGTGGTTCTC GACACACTGG AGCAGCTCAA AAGTGGCGGG 
ACATCTGAAC CATCCAGCCC TCTCCATTCC CGCCTGCTCC GCGAAGCTGA ATCCTCTCAG 
CATCCTCTGC AGCGCAGCGC CAGTTCAGCC AGCGACATGC CCTCCACTTT CCGACCCAGC 
AAAACCACAG GCCCCAAGAG CCCTCTGTCC TCCATGACCA CCGCTTCTGG CTCCACATTC 
AGAGATAACA AACCTCCAGC CACCAGACCC AAGCCTGTGG TGTTCCCCAA AAGCAGTTCA 
GCCGGAGGAA GTCCGGCTAT GGGATCTCCA ACCACCACCA TCCCACCAAC ACCACCACCT 
CCACCACCAC CCACCGACAA ATCCTGCCCC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172933.1
CDS	477..3752
Translation	MTSPAKFRKDKEIIAEYETQVKEIRAQLVEQLKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKLAERFLAKTRYTKDPQFKKEQNILSPVNCWNLLLAQVKRESRDHATLSDLYLNNIIPRFAQISEDSGRLFKKSKEVGLQLQEDLMKVLNELYTVMKTYHMYNMDSINAESKLKEAEKQEEKQMSRSVRHEEKQTPRSPDSLTNIKIEDKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNSCVFKYYIHDLSDLIDCCDLGYHASLNRALRTYLSAEFNVETSKHGGLETIENAAENLEANSDKQRLMETYNNVFCPPMRFDFQSHMGDMVGHLCAQQPVQGELIQRCQQLQSRLSTLNIENEEVKKTMEATLQTIQDMVTIEDFDVTDCFHHSNSMESVKSTVSESFMSKPSLAKRRANQQETEQFYFTKLKEFLEGRNLITKLQAKHDLIQKTLGESQKTDYCLASGRRDSTVRKQEAIQIIPLMVESCIRFISRHGLHHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLENALFPKEVFHDLMSCVSIESLQERAVHIRKVLLSLPSNILVIMRYLFAFLNHLSQYSDDNMMDPYNLAICFGPTLMSVPEGHDQVSCQAHVNELIKTIIIHHESIFPGPRELEGPVYDRGGAPEEYCDSPHIEPPLVDEPAPDTVSVIHNSDDESDPIEAIARFDYSGRTNRELSFKKGASLLLYSRASDDWWEGRHNGTEGLVPHQYIVVQDMPDGYAGRGSPKADLEGSHDSVEEKVSTRASASSPTGGHVADIYLANLNKLRKRPEATSIRRTIRPVEEGSSGAAGGLKTSSMPAGGLAKDSSDKRPVSAHSVLNSITRHSSLKTKVEGPQVRKSTPTGRSKSFSNHRPLDPEVIAQVEHSSQDIEATMNTALSELRELERQSNVKHAPDVVLDTLEQLKSGGTSEPSSPLHSRLLREAESSQHPLQRSASSASDMPSTFRPSKTTGPKSPLSSMTTASGSTFRDNKPPATRPKPVVFPKSSSAGGSPAMGSPTTTIPPTPPPPPPPTDKSCPV

Target ORF information:

RefSeq Version	XM_005172876.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172876.3

ORF Insert Sequence:

ATGACGTCTC CTGCTAAATT CCGAAAGGAC AAGGAAATTA TAGCCGAATA TGAAACGCAA 
GTTAAAGAGA TCCGAGCTCA GTTGGTGGAG CAGCTTAAGT GTTTGGACCA GCAATGTGAG 
CTCCGTGTGC AGCTCCTGCA AGACCTGCAG GACTTCTTCC GCAAGAAGGC AGAGATTGAG 
ATGGACTACT CCCGCAACCT AGAGAAACTA GCTGAGAGGT TCCTGGCAAA GACACGCTAC 
ACAAAAGACC CTCAATTCAA AAAAGAGCAG AATATCTTGT CTCCAGTCAA CTGCTGGAAC 
CTTCTCTTGG CTCAGGTGAA GAGAGAAAGT CGAGACCATG CCACTCTCAG TGATCTCTAC 
CTCAACAACA TCATCCCACG CTTCGCCCAG ATCAGCGAAG ACTCAGGACG CCTCTTTAAA 
AAGAGCAAAG AGGTGGGCCT CCAGCTGCAG GAGGATCTGA TGAAAGTCCT CAATGAGTTG 
TACACGGTGA TGAAGACTTA TCACATGTAC AACATGGACA GCATCAACGC AGAGAGCAAG 
CTGAAGGAGG CAGAGAAGCA GGAGGAGAAA CAGATGAGCC GCAGTGTCCG GCATGAGGAA 
AAACAGACCC CTCGCTCACC TGACTCTCTC ACCAACATCA AGATCGAGGA TAAACACGTG 
CGCCGCAGCT CCGTCAAGAA GATTGAGAAG ATGAAGGAGA AGCGGCAGGC CAAATACACT 
GAGAATAAGC TGAAAGCTAT CAAGGCCAGG AATGAGTATC TTCTGGCTCT GGAGGCCACA 
AACAGCTGCG TCTTCAAATA CTACATCCAT GATCTGTCTG ACCTCATCGA TTGCTGTGAT 
CTGGGCTACC ATGCCAGCCT GAACCGTGCA CTGCGTACCT ACCTCTCGGC TGAGTTTAAC 
GTGGAGACAT CCAAACACGG GGGCCTGGAG ACCATTGAGA ATGCAGCAGA AAACCTGGAG 
GCCAACTCAG ACAAACAGCG GCTCATGGAG ACCTACAACA ACGTCTTCTG TCCTCCGATG 
CGCTTCGATT TCCAGTCTCA CATGGGTGAC ATGGTTGGAC ATTTGTGTGC CCAGCAGCCT 
GTGCAGGGAG AGCTGATCCA GAGATGCCAG CAGCTTCAGT CCCGTCTGTC CACACTGAAT 
ATAGAGAATG AAGAGGTTAA GAAGACCATG GAGGCCACTT TACAGACCAT CCAGGACATG 
GTGACCATAG AGGACTTCGA TGTGACCGAC TGCTTCCACC ACAGCAACTC AATGGAGTCG 
GTCAAGTCCA CAGTGTCTGA GTCTTTCATG AGCAAACCCA GCCTGGCCAA GAGACGAGCC 
AACCAGCAGG AGACCGAACA GTTCTACTTC ACGAAACTGA AGGAGTTTCT GGAGGGCAGG 
AACCTCATCA CCAAACTGCA GGCTAAACAC GATCTGATCC AAAAGACTCT GGGAGAAAGT 
CAGAAGACGG ACTACTGCCT TGCCAGCGGA CGGAGGGACT CCACTGTGCG GAAACAGGAG 
GCCATCCAAA TCATTCCCCT GATGGTGGAG AGTTGTATTC GCTTCATCAG TCGACACGGT 
CTCCATCATG AGGGGATTTT CAGGGTGTCT GGCTCCCAGG TGGAGGTCAA TGACATTAAA 
AACGCCTTTG AAAGAGGTGA GGATCCGCTG GCTGGAGATC AGAATGATCA CGATATGGAC 
TCCATCGCTG GAGTGCTGAA GCTCTACTTC AGAGGCCTGG AAAACGCCCT GTTTCCTAAG 
GAAGTCTTCC ATGACCTCAT GTCCTGTGTC TCCATTGAGA GCCTCCAGGA AAGAGCTGTG 
CACATCCGAA AAGTCCTGCT GTCGCTGCCC AGCAACATCC TGGTTATCAT GAGATACCTG 
TTTGCTTTTC TCAATCATCT GTCTCAGTAC AGTGACGACA ACATGATGGA CCCCTACAAC 
CTGGCTATCT GCTTCGGCCC CACACTCATG TCTGTTCCTG AGGGCCACGA TCAAGTCTCC 
TGCCAGGCAC ATGTGAACGA GCTCATCAAA ACCATTATTA TCCACCACGA GAGCATCTTT 
CCAGGGCCCC GTGAGCTGGA GGGCCCCGTC TACGACCGTG GAGGAGCCCC TGAGGAATAC 
TGTGATAGTC CACACATTGA GCCCCCTCTA GTGGACGAAC CTGCTCCTGA CACGGTCTCT 
GTAATCCACA ACAGTGATGA CGAGTCGGAC CCCATCGAGG CCATCGCTCG CTTCGATTAC 
TCTGGACGCA CCAACCGCGA GCTGAGCTTC AAAAAGGGGG CGTCTCTGCT GCTGTACAGC 
CGCGCATCTG ATGACTGGTG GGAGGGACGA CACAACGGCA CTGAGGGTCT CGTGCCGCAC 
CAGTACATAG TAGTGCAGGA CATGCCGGAT GGATATGCAG GCAGAGGAAG TCCTAAAGCT 
GACCTCGAAG GATCCCATGA TTCAGTGGAA GAGAAGGTTT CCACGAGAGC CAGTGCCAGC 
TCACCCACTG GAGGTCATGT GGCTGATATC TATCTAGCCA ACCTCAACAA GTTGAGGAAG 
CGTCCAGAAG CGACGAGCAT CCGGAGAACC ATCCGGCCTG TGGAGGAGGG CTCATCAGGA 
GCAGCAGGGG GCTTAAAGAC CTCCTCTATG CCTGCTGGAG GACTGGCCAA GGACAGCTCA 
GACAAGCGTC CTGTCAGCGC TCACAGCGTC CTGAACTCCA TCACCAGACA CTCGTCTCTC 
AAGACTAAAG TAGAGGGTCC TCAAGTTCGC AAATCCACAC CTACTGGACG ATCCAAGAGC 
TTCAGCAACC ACAGACCCCT CGACCCAGAG GTGATCGCGC AGGTGGAGCA CAGCTCACAG 
GACATCGAGG CCACTATGAA CACGGCTCTG AGTGAGCTGC GAGAGCTTGA GAGGCAGAGC 
AACGTCAAAC ATGCTCCAGA CGTGGTTCTC GACACACTGG AGCAGCTCAA AAGTGGCGGG 
ACATCTGAAC CATCCAGCCC TCTCCATTCC CGCCTGCTCC GCGAAGCTGA ATCCTCTCAG 
CATCCTCTGC AGCGCAGCGC CAGTTCAGCC AGCGACATGC CCTCCACTTT CCGACCCAGC 
AAAACCACAG GCCCCAAGAG CCCTCTGTCC TCCATGACCA CCGCTTCTGG CTCCACATTC 
AGAGATAACA AACCTCCAGC CACCAGACCC AAGCCTGTGG TGTTCCCCAA AAGCAGTTCA 
GCCGGAGGAA GTCCGGCTAT GGGATCTCCA ACCACCACCA TCCCACCAAC ACCACCACCT 
CCACCACCAC CCACCGACAA ATCCTGCCCC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRGAP2 ( XM_009305994.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304269.1 Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42319
Clone ID Related Accession (Same CDS sequence)	XM_009305994.2
Accession Version	XM_009305994.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SLIT-ROBO Rho GTPase-activating protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305994.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCTC CTGCTAAATT CCGAAAGGAC AAGGAAATTA TAGCCGAATA TGAAACGCAA 
GTTAAAGAGA TCCGAGCTCA GTTGGTGGAG CAGCTTAAGT GTTTGGACCA GCAATGTGAG 
CTCCGTGTGC AGCTCCTGCA AGACCTGCAG GACTTCTTCC GCAAGAAGGC AGAGATTGAG 
ATGGACTACT CCCGCAACCT AGAGAAACTA GCTGAGAGGT TCCTGGCAAA GACACGCTAC 
ACAAAAGACC CTCAATTCAA AAAAGAGCAG AATATCTTGT CTCCAGTCAA CTGCTGGAAC 
CTTCTCTTGG CTCAGGTGAA GAGAGAAAGT CGAGACCATG CCACTCTCAG TGATCTCTAC 
CTCAACAACA TCATCCCACG CTTCGCCCAG ATCAGCGAAG ACTCAGGACG CCTCTTTAAA 
AAGAGCAAAG AGGTGGGCCT CCAGCTGCAG GAGGATCTGA TGAAAGTCCT CAATGAGTTG 
TACACGGTGA TGAAGACTTA TCACATGTAC AACATGGACA GCATCAACGC AGAGAGCAAG 
CTGAAGGAGG CAGAGAAGCA GGAGGAGAAA CAGATGAGCC GCAGTGTCCG GCATGAGGAA 
AAACAGACCC CTCGCTCACC TGACTCTCTC ACCAACATCA AGATCGAGGA TAAACACGTG 
CGCCGCAGCT CCGTCAAGAA GATTGAGAAG ATGAAGGAGA AGCGGCAGGC CAAATACACT 
GAGAATAAGC TGAAAGCTAT CAAGGCCAGG AATGAGTATC TTCTGGCTCT GGAGGCCACA 
AACAGCTGCG TCTTCAAATA CTACATCCAT GATCTGTCTG ACCTCATCGA TTGCTGTGAT 
CTGGGCTACC ATGCCAGCCT GAACCGTGCA CTGCGTACCT ACCTCTCGGC TGAGTTTAAC 
GTGGAGACAT CCAAACACGG GGGCCTGGAG ACCATTGAGA ATGCAGCAGA AAACCTGGAG 
GCCAACTCAG ACAAACAGCG GCTCATGGAG ACCTACAACA ACGTCTTCTG TCCTCCGATG 
CGCTTCGATT TCCAGTCTCA CATGGGTGAC ATGGTTGGAC ATTTGTGTGC CCAGCAGCCT 
GTGCAGGGAG AGCTGATCCA GAGATGCCAG CAGCTTCAGT CCCGTCTGTC CACACTGAAT 
ATAGAGAATG AAGAGGTTAA GAAGACCATG GAGGCCACTT TACAGACCAT CCAGGACATG 
GTGACCATAG AGGACTTCGA TGTGACCGAC TGCTTCCACC ACAGCAACTC AATGGAGTCG 
GTCAAGTCCA CAGTGTCTGA GTCTTTCATG AGCAAACCCA GCCTGGCCAA GAGACGAGCC 
AACCAGCAGG AGACCGAACA GTTCTACTTC ACGAAACTGA AGGAGTTTCT GGAGGGCAGG 
AACCTCATCA CCAAACTGCA GGCTAAACAC GATCTGATCC AAAAGACTCT GGGAGAAAGT 
CAGAAGACGG ACTACTGCCT TGCCAGCGGA CGGAGGGACT CCACTGTGCG GAAACAGGAG 
GCCATCCAAA TCATTCCCCT GATGGTGGAG AGTTGTATTC GCTTCATCAG TCGACACGGT 
CTCCATCATG AGGGGATTTT CAGGGTGTCT GGCTCCCAGG TGGAGGTCAA TGACATTAAA 
AACGCCTTTG AAAGAGGTGA GGATCCGCTG GCTGGAGATC AGAATGATCA CGATATGGAC 
TCCATCGCTG GAGTGCTGAA GCTCTACTTC AGAGGCCTGG AAAACGCCCT GTTTCCTAAG 
GAAGTCTTCC ATGACCTCAT GTCCTGTGTC TCCATTGAGA GCCTCCAGGA AAGAGCTGTG 
CACATCCGAA AAGTCCTGCT GTCGCTGCCC AGCAACATCC TGGTTATCAT GAGATACCTG 
TTTGCTTTTC TCAATCATCT GTCTCAGTAC AGTGACGACA ACATGATGGA CCCCTACAAC 
CTGGCTATCT GCTTCGGCCC CACACTCATG TCTGTTCCTG AGGGCCACGA TCAAGTCTCC 
TGCCAGGCAC ATGTGAACGA GCTCATCAAA ACCATTATTA TCCACCACGA GAGCATCTTT 
CCAGGGCCCC GTGAGCTGGA GGGCCCCGTC TACGACCGTG GAGGAGCCCC TGAGGAATAC 
TGTGATAGTC CACACATTGA GCCCCCTCTA GTGGACGAAC CTGCTCCTGA CACGGTCTCT 
GTAATCCACA ACAGTGATGA CGTAAAGTCA GGGCCATTGA CCGTTTCAGA GTCGGACCCC 
ATCGAGGCCA TCGCTCGCTT CGATTACTCT GGACGCACCA ACCGCGAGCT GAGCTTCAAA 
AAGGGGGCGT CTCTGCTGCT GTACAGCCGC GCATCTGATG ACTGGTGGGA GGGACGACAC 
AACGGCACTG AGGGTCTCGT GCCGCACCAG TACATAGTAG TGCAGGACAT GCCGGATGGA 
TATGCAGGCA GAGGAAGTCC TAAAGCTGAC CTCGAAGGAT CCCATGATTC AGTGGAAGAG 
AAGGTTTCCA CGAGAGCCAG TGCCAGCTCA CCCACTGGAG GTCATGTGGC TGATATCTAT 
CTAGCCAACC TCAACAAGTT GAGGAAGCGT CCAGAAGCGA CGAGCATCCG GAGAACCATC 
CGGCCTGTGG AGGAGGGCTC ATCAGGAGCA GCAGGGGGCT TAAAGACCTC CTCTATGCCT 
GCTGGAGGAC TGGCCAAGGA CAGCTCAGAC AAGCGTCCTG TCAGCGCTCA CAGCGTCCTG 
AACTCCATCA CCAGACACTC GTCTCTCAAG ACTAAAGTAG AGGGTCCTCA AGTTCGCAAA 
TCCACACCTA CTGGACGATC CAAGAGCTTC AGCAACCACA GACCCCTCGA CCCAGAGGTG 
ATCGCGCAGG TGGAGCACAG CTCACAGGAC ATCGAGGCCA CTATGAACAC GGCTCTGAGT 
GAGCTGCGAG AGCTTGAGAG GCAGAGCAAC GTCAAACATG CTCCAGACGT GGTTCTCGAC 
ACACTGGAGC AGCTCAAAAG TGGCGGGACA TCTGAACCAT CCAGCCCTCT CCATTCCCGC 
CTGCTCCGCG AAGCTGAATC CTCTCAGCAT CCTCTGCAGC GCAGCGCCAG TTCAGCCAGC 
GACATGCCCT CCACTTTCCG ACCCAGCAAA ACCACAGGCC CCAAGAGCCC TCTGTCCTCC 
ATGACCACCG CTTCTGGCTC CACATTCAGA GATAACAAAC CTCCAGCCAC CAGACCCAAG 
CCTGTGGTGT TCCCCAAAAG CAGTTCAGCC GGAGGAAGTC CGGCTATGGG ATCTCCAACC 
ACCACCATCC CACCAACACC ACCACCTCCA CCACCACCCA CCGACAAATC CTGCCCCGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304269.1
CDS	440..3742
Translation	MTSPAKFRKDKEIIAEYETQVKEIRAQLVEQLKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKLAERFLAKTRYTKDPQFKKEQNILSPVNCWNLLLAQVKRESRDHATLSDLYLNNIIPRFAQISEDSGRLFKKSKEVGLQLQEDLMKVLNELYTVMKTYHMYNMDSINAESKLKEAEKQEEKQMSRSVRHEEKQTPRSPDSLTNIKIEDKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNSCVFKYYIHDLSDLIDCCDLGYHASLNRALRTYLSAEFNVETSKHGGLETIENAAENLEANSDKQRLMETYNNVFCPPMRFDFQSHMGDMVGHLCAQQPVQGELIQRCQQLQSRLSTLNIENEEVKKTMEATLQTIQDMVTIEDFDVTDCFHHSNSMESVKSTVSESFMSKPSLAKRRANQQETEQFYFTKLKEFLEGRNLITKLQAKHDLIQKTLGESQKTDYCLASGRRDSTVRKQEAIQIIPLMVESCIRFISRHGLHHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLENALFPKEVFHDLMSCVSIESLQERAVHIRKVLLSLPSNILVIMRYLFAFLNHLSQYSDDNMMDPYNLAICFGPTLMSVPEGHDQVSCQAHVNELIKTIIIHHESIFPGPRELEGPVYDRGGAPEEYCDSPHIEPPLVDEPAPDTVSVIHNSDDVKSGPLTVSESDPIEAIARFDYSGRTNRELSFKKGASLLLYSRASDDWWEGRHNGTEGLVPHQYIVVQDMPDGYAGRGSPKADLEGSHDSVEEKVSTRASASSPTGGHVADIYLANLNKLRKRPEATSIRRTIRPVEEGSSGAAGGLKTSSMPAGGLAKDSSDKRPVSAHSVLNSITRHSSLKTKVEGPQVRKSTPTGRSKSFSNHRPLDPEVIAQVEHSSQDIEATMNTALSELRELERQSNVKHAPDVVLDTLEQLKSGGTSEPSSPLHSRLLREAESSQHPLQRSASSASDMPSTFRPSKTTGPKSPLSSMTTASGSTFRDNKPPATRPKPVVFPKSSSAGGSPAMGSPTTTIPPTPPPPPPPTDKSCPV

Target ORF information:

RefSeq Version	XM_009305994.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305994.2

ORF Insert Sequence:

ATGACGTCTC CTGCTAAATT CCGAAAGGAC AAGGAAATTA TAGCCGAATA TGAAACGCAA 
GTTAAAGAGA TCCGAGCTCA GTTGGTGGAG CAGCTTAAGT GTTTGGACCA GCAATGTGAG 
CTCCGTGTGC AGCTCCTGCA AGACCTGCAG GACTTCTTCC GCAAGAAGGC AGAGATTGAG 
ATGGACTACT CCCGCAACCT AGAGAAACTA GCTGAGAGGT TCCTGGCAAA GACACGCTAC 
ACAAAAGACC CTCAATTCAA AAAAGAGCAG AATATCTTGT CTCCAGTCAA CTGCTGGAAC 
CTTCTCTTGG CTCAGGTGAA GAGAGAAAGT CGAGACCATG CCACTCTCAG TGATCTCTAC 
CTCAACAACA TCATCCCACG CTTCGCCCAG ATCAGCGAAG ACTCAGGACG CCTCTTTAAA 
AAGAGCAAAG AGGTGGGCCT CCAGCTGCAG GAGGATCTGA TGAAAGTCCT CAATGAGTTG 
TACACGGTGA TGAAGACTTA TCACATGTAC AACATGGACA GCATCAACGC AGAGAGCAAG 
CTGAAGGAGG CAGAGAAGCA GGAGGAGAAA CAGATGAGCC GCAGTGTCCG GCATGAGGAA 
AAACAGACCC CTCGCTCACC TGACTCTCTC ACCAACATCA AGATCGAGGA TAAACACGTG 
CGCCGCAGCT CCGTCAAGAA GATTGAGAAG ATGAAGGAGA AGCGGCAGGC CAAATACACT 
GAGAATAAGC TGAAAGCTAT CAAGGCCAGG AATGAGTATC TTCTGGCTCT GGAGGCCACA 
AACAGCTGCG TCTTCAAATA CTACATCCAT GATCTGTCTG ACCTCATCGA TTGCTGTGAT 
CTGGGCTACC ATGCCAGCCT GAACCGTGCA CTGCGTACCT ACCTCTCGGC TGAGTTTAAC 
GTGGAGACAT CCAAACACGG GGGCCTGGAG ACCATTGAGA ATGCAGCAGA AAACCTGGAG 
GCCAACTCAG ACAAACAGCG GCTCATGGAG ACCTACAACA ACGTCTTCTG TCCTCCGATG 
CGCTTCGATT TCCAGTCTCA CATGGGTGAC ATGGTTGGAC ATTTGTGTGC CCAGCAGCCT 
GTGCAGGGAG AGCTGATCCA GAGATGCCAG CAGCTTCAGT CCCGTCTGTC CACACTGAAT 
ATAGAGAATG AAGAGGTTAA GAAGACCATG GAGGCCACTT TACAGACCAT CCAGGACATG 
GTGACCATAG AGGACTTCGA TGTGACCGAC TGCTTCCACC ACAGCAACTC AATGGAGTCG 
GTCAAGTCCA CAGTGTCTGA GTCTTTCATG AGCAAACCCA GCCTGGCCAA GAGACGAGCC 
AACCAGCAGG AGACCGAACA GTTCTACTTC ACGAAACTGA AGGAGTTTCT GGAGGGCAGG 
AACCTCATCA CCAAACTGCA GGCTAAACAC GATCTGATCC AAAAGACTCT GGGAGAAAGT 
CAGAAGACGG ACTACTGCCT TGCCAGCGGA CGGAGGGACT CCACTGTGCG GAAACAGGAG 
GCCATCCAAA TCATTCCCCT GATGGTGGAG AGTTGTATTC GCTTCATCAG TCGACACGGT 
CTCCATCATG AGGGGATTTT CAGGGTGTCT GGCTCCCAGG TGGAGGTCAA TGACATTAAA 
AACGCCTTTG AAAGAGGTGA GGATCCGCTG GCTGGAGATC AGAATGATCA CGATATGGAC 
TCCATCGCTG GAGTGCTGAA GCTCTACTTC AGAGGCCTGG AAAACGCCCT GTTTCCTAAG 
GAAGTCTTCC ATGACCTCAT GTCCTGTGTC TCCATTGAGA GCCTCCAGGA AAGAGCTGTG 
CACATCCGAA AAGTCCTGCT GTCGCTGCCC AGCAACATCC TGGTTATCAT GAGATACCTG 
TTTGCTTTTC TCAATCATCT GTCTCAGTAC AGTGACGACA ACATGATGGA CCCCTACAAC 
CTGGCTATCT GCTTCGGCCC CACACTCATG TCTGTTCCTG AGGGCCACGA TCAAGTCTCC 
TGCCAGGCAC ATGTGAACGA GCTCATCAAA ACCATTATTA TCCACCACGA GAGCATCTTT 
CCAGGGCCCC GTGAGCTGGA GGGCCCCGTC TACGACCGTG GAGGAGCCCC TGAGGAATAC 
TGTGATAGTC CACACATTGA GCCCCCTCTA GTGGACGAAC CTGCTCCTGA CACGGTCTCT 
GTAATCCACA ACAGTGATGA CGTAAAGTCA GGGCCATTGA CCGTTTCAGA GTCGGACCCC 
ATCGAGGCCA TCGCTCGCTT CGATTACTCT GGACGCACCA ACCGCGAGCT GAGCTTCAAA 
AAGGGGGCGT CTCTGCTGCT GTACAGCCGC GCATCTGATG ACTGGTGGGA GGGACGACAC 
AACGGCACTG AGGGTCTCGT GCCGCACCAG TACATAGTAG TGCAGGACAT GCCGGATGGA 
TATGCAGGCA GAGGAAGTCC TAAAGCTGAC CTCGAAGGAT CCCATGATTC AGTGGAAGAG 
AAGGTTTCCA CGAGAGCCAG TGCCAGCTCA CCCACTGGAG GTCATGTGGC TGATATCTAT 
CTAGCCAACC TCAACAAGTT GAGGAAGCGT CCAGAAGCGA CGAGCATCCG GAGAACCATC 
CGGCCTGTGG AGGAGGGCTC ATCAGGAGCA GCAGGGGGCT TAAAGACCTC CTCTATGCCT 
GCTGGAGGAC TGGCCAAGGA CAGCTCAGAC AAGCGTCCTG TCAGCGCTCA CAGCGTCCTG 
AACTCCATCA CCAGACACTC GTCTCTCAAG ACTAAAGTAG AGGGTCCTCA AGTTCGCAAA 
TCCACACCTA CTGGACGATC CAAGAGCTTC AGCAACCACA GACCCCTCGA CCCAGAGGTG 
ATCGCGCAGG TGGAGCACAG CTCACAGGAC ATCGAGGCCA CTATGAACAC GGCTCTGAGT 
GAGCTGCGAG AGCTTGAGAG GCAGAGCAAC GTCAAACATG CTCCAGACGT GGTTCTCGAC 
ACACTGGAGC AGCTCAAAAG TGGCGGGACA TCTGAACCAT CCAGCCCTCT CCATTCCCGC 
CTGCTCCGCG AAGCTGAATC CTCTCAGCAT CCTCTGCAGC GCAGCGCCAG TTCAGCCAGC 
GACATGCCCT CCACTTTCCG ACCCAGCAAA ACCACAGGCC CCAAGAGCCC TCTGTCCTCC 
ATGACCACCG CTTCTGGCTC CACATTCAGA GATAACAAAC CTCCAGCCAC CAGACCCAAG 
CCTGTGGTGT TCCCCAAAAG CAGTTCAGCC GGAGGAAGTC CGGCTATGGG ATCTCCAACC 
ACCACCATCC CACCAACACC ACCACCTCCA CCACCACCCA CCGACAAATC CTGCCCCGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRGAP2 ( XM_009305995.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304270.1 Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa42320
Clone ID Related Accession (Same CDS sequence)	XM_009305995.2
Accession Version	XM_009305995.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SLIT-ROBO Rho GTPase-activating protein 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305995.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAA AAGTCATCCG ATTTCTCTAT AGCAGGAAAT GCAAAGAGAT CCGAGCTCAG 
TTGGTGGAGC AGCTTAAGTG TTTGGACCAG CAATGTGAGC TCCGTGTGCA GCTCCTGCAA 
GACCTGCAGG ACTTCTTCCG CAAGAAGGCA GAGATTGAGA TGGACTACTC CCGCAACCTA 
GAGAAACTAG CTGAGAGGTT CCTGGCAAAG ACACGCTACA CAAAAGACCC TCAATTCAAA 
AAAGAGCAGA ATATCTTGTC TCCAGTCAAC TGCTGGAACC TTCTCTTGGC TCAGGTGAAG 
AGAGAAAGTC GAGACCATGC CACTCTCAGT GATCTCTACC TCAACAACAT CATCCCACGC 
TTCGCCCAGA TCAGCGAAGA CTCAGGACGC CTCTTTAAAA AGAGCAAAGA GGTGGGCCTC 
CAGCTGCAGG AGGATCTGAT GAAAGTCCTC AATGAGTTGT ACACGGTGAT GAAGACTTAT 
CACATGTACA ACATGGACAG CATCAACGCA GAGAGCAAGC TGAAGGAGGC AGAGAAGCAG 
GAGGAGAAAC AGATGAGCCG CAGTGTCCGG CATGAGGAAA AACAGACCCC TCGCTCACCT 
GACTCTCTCA CCAACATCAA GATCGAGGAT AAACACGTGC GCCGCAGCTC CGTCAAGAAG 
ATTGAGAAGA TGAAGGAGAA GCGGCAGGCC AAATACACTG AGAATAAGCT GAAAGCTATC 
AAGGCCAGGA ATGAGTATCT TCTGGCTCTG GAGGCCACAA ACAGCTGCGT CTTCAAATAC 
TACATCCATG ATCTGTCTGA CCTCATCGAT TGCTGTGATC TGGGCTACCA TGCCAGCCTG 
AACCGTGCAC TGCGTACCTA CCTCTCGGCT GAGTTTAACG TGGAGACATC CAAACACGGG 
GGCCTGGAGA CCATTGAGAA TGCAGCAGAA AACCTGGAGG CCAACTCAGA CAAACAGCGG 
CTCATGGAGA CCTACAACAA CGTCTTCTGT CCTCCGATGC GCTTCGATTT CCAGTCTCAC 
ATGGGTGACA TGGTTGGACA TTTGTGTGCC CAGCAGCCTG TGCAGGGAGA GCTGATCCAG 
AGATGCCAGC AGCTTCAGTC CCGTCTGTCC ACACTGAATA TAGAGAATGA AGAGGTTAAG 
AAGACCATGG AGGCCACTTT ACAGACCATC CAGGACATGG TGACCATAGA GGACTTCGAT 
GTGACCGACT GCTTCCACCA CAGCAACTCA ATGGAGTCGG TCAAGTCCAC AGTGTCTGAG 
TCTTTCATGA GCAAACCCAG CCTGGCCAAG AGACGAGCCA ACCAGCAGGA GACCGAACAG 
TTCTACTTCA CGAAACTGAA GGAGTTTCTG GAGGGCAGGA ACCTCATCAC CAAACTGCAG 
GCTAAACACG ATCTGATCCA AAAGACTCTG GGAGAAAGTC AGAAGACGGA CTACTGCCTT 
GCCAGCGGAC GGAGGGACTC CACTGTGCGG AAACAGGAGG CCATCCAAAT CATTCCCCTG 
ATGGTGGAGA GTTGTATTCG CTTCATCAGT CGACACGGTC TCCATCATGA GGGGATTTTC 
AGGGTGTCTG GCTCCCAGGT GGAGGTCAAT GACATTAAAA ACGCCTTTGA AAGAGGTGAG 
GATCCGCTGG CTGGAGATCA GAATGATCAC GATATGGACT CCATCGCTGG AGTGCTGAAG 
CTCTACTTCA GAGGCCTGGA AAACGCCCTG TTTCCTAAGG AAGTCTTCCA TGACCTCATG 
TCCTGTGTCT CCATTGAGAG CCTCCAGGAA AGAGCTGTGC ACATCCGAAA AGTCCTGCTG 
TCGCTGCCCA GCAACATCCT GGTTATCATG AGATACCTGT TTGCTTTTCT CAATCATCTG 
TCTCAGTACA GTGACGACAA CATGATGGAC CCCTACAACC TGGCTATCTG CTTCGGCCCC 
ACACTCATGT CTGTTCCTGA GGGCCACGAT CAAGTCTCCT GCCAGGCACA TGTGAACGAG 
CTCATCAAAA CCATTATTAT CCACCACGAG AGCATCTTTC CAGGGCCCCG TGAGCTGGAG 
GGCCCCGTCT ACGACCGTGG AGGAGCCCCT GAGGAATACT GTGATAGTCC ACACATTGAG 
CCCCCTCTAG TGGACGAACC TGCTCCTGAC ACGGTCTCTG TAATCCACAA CAGTGATGAC 
GTAAAGTCAG GGCCATTGAC CGTTTCAGAG TCGGACCCCA TCGAGGCCAT CGCTCGCTTC 
GATTACTCTG GACGCACCAA CCGCGAGCTG AGCTTCAAAA AGGGGGCGTC TCTGCTGCTG 
TACAGCCGCG CATCTGATGA CTGGTGGGAG GGACGACACA ACGGCACTGA GGGTCTCGTG 
CCGCACCAGT ACATAGTAGT GCAGGACATG CCGGATGGAT ATGCAGGCAG AGGAAGTCCT 
AAAGCTGACC TCGAAGGATC CCATGATTCA GTGGAAGAGA AGGTTTCCAC GAGAGCCAGT 
GCCAGCTCAC CCACTGGAGG TCATGTGGCT GATATCTATC TAGCCAACCT CAACAAGTTG 
AGGAAGCGTC CAGAAGCGAC GAGCATCCGG AGAACCATCC GGCCTGTGGA GGAGGGCTCA 
TCAGGAGCAG CAGGGGGCTT AAAGACCTCC TCTATGCCTG CTGGAGGACT GGCCAAGGAC 
AGCTCAGACA AGCGTCCTGT CAGCGCTCAC AGCGTCCTGA ACTCCATCAC CAGACACTCG 
TCTCTCAAGA CTAAAGTAGA GGGTCCTCAA GTTCGCAAAT CCACACCTAC TGGACGATCC 
AAGAGCTTCA GCAACCACAG ACCCCTCGAC CCAGAGGTGA TCGCGCAGGT GGAGCACAGC 
TCACAGGACA TCGAGGCCAC TATGAACACG GCTCTGAGTG AGCTGCGAGA GCTTGAGAGG 
CAGAGCAACG TCAAACATGC TCCAGACGTG GTTCTCGACA CACTGGAGCA GCTCAAAAGT 
GGCGGGACAT CTGAACCATC CAGCCCTCTC CATTCCCGCC TGCTCCGCGA AGCTGAATCC 
TCTCAGCATC CTCTGCAGCG CAGCGCCAGT TCAGCCAGCG ACATGCCCTC CACTTTCCGA 
CCCAGCAAAA CCACAGGCCC CAAGAGCCCT CTGTCCTCCA TGACCACCGC TTCTGGCTCC 
ACATTCAGAG ATAACAAACC TCCAGCCACC AGACCCAAGC CTGTGGTGTT CCCCAAAAGC 
AGTTCAGCCG GAGGAAGTCC GGCTATGGGA TCTCCAACCA CCACCATCCC ACCAACACCA 
CCACCTCCAC CACCACCCAC CGACAAATCC TGCCCCGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304270.1
CDS	169..3450
Translation	MEAKVIRFLYSRKCKEIRAQLVEQLKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKLAERFLAKTRYTKDPQFKKEQNILSPVNCWNLLLAQVKRESRDHATLSDLYLNNIIPRFAQISEDSGRLFKKSKEVGLQLQEDLMKVLNELYTVMKTYHMYNMDSINAESKLKEAEKQEEKQMSRSVRHEEKQTPRSPDSLTNIKIEDKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNSCVFKYYIHDLSDLIDCCDLGYHASLNRALRTYLSAEFNVETSKHGGLETIENAAENLEANSDKQRLMETYNNVFCPPMRFDFQSHMGDMVGHLCAQQPVQGELIQRCQQLQSRLSTLNIENEEVKKTMEATLQTIQDMVTIEDFDVTDCFHHSNSMESVKSTVSESFMSKPSLAKRRANQQETEQFYFTKLKEFLEGRNLITKLQAKHDLIQKTLGESQKTDYCLASGRRDSTVRKQEAIQIIPLMVESCIRFISRHGLHHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLENALFPKEVFHDLMSCVSIESLQERAVHIRKVLLSLPSNILVIMRYLFAFLNHLSQYSDDNMMDPYNLAICFGPTLMSVPEGHDQVSCQAHVNELIKTIIIHHESIFPGPRELEGPVYDRGGAPEEYCDSPHIEPPLVDEPAPDTVSVIHNSDDVKSGPLTVSESDPIEAIARFDYSGRTNRELSFKKGASLLLYSRASDDWWEGRHNGTEGLVPHQYIVVQDMPDGYAGRGSPKADLEGSHDSVEEKVSTRASASSPTGGHVADIYLANLNKLRKRPEATSIRRTIRPVEEGSSGAAGGLKTSSMPAGGLAKDSSDKRPVSAHSVLNSITRHSSLKTKVEGPQVRKSTPTGRSKSFSNHRPLDPEVIAQVEHSSQDIEATMNTALSELRELERQSNVKHAPDVVLDTLEQLKSGGTSEPSSPLHSRLLREAESSQHPLQRSASSASDMPSTFRPSKTTGPKSPLSSMTTASGSTFRDNKPPATRPKPVVFPKSSSAGGSPAMGSPTTTIPPTPPPPPPPTDKSCPV

Target ORF information:

RefSeq Version	XM_009305995.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SLIT-ROBO Rho GTPase activating protein 2 (srgap2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305995.2

ORF Insert Sequence:

ATGGAGGCAA AAGTCATCCG ATTTCTCTAT AGCAGGAAAT GCAAAGAGAT CCGAGCTCAG 
TTGGTGGAGC AGCTTAAGTG TTTGGACCAG CAATGTGAGC TCCGTGTGCA GCTCCTGCAA 
GACCTGCAGG ACTTCTTCCG CAAGAAGGCA GAGATTGAGA TGGACTACTC CCGCAACCTA 
GAGAAACTAG CTGAGAGGTT CCTGGCAAAG ACACGCTACA CAAAAGACCC TCAATTCAAA 
AAAGAGCAGA ATATCTTGTC TCCAGTCAAC TGCTGGAACC TTCTCTTGGC TCAGGTGAAG 
AGAGAAAGTC GAGACCATGC CACTCTCAGT GATCTCTACC TCAACAACAT CATCCCACGC 
TTCGCCCAGA TCAGCGAAGA CTCAGGACGC CTCTTTAAAA AGAGCAAAGA GGTGGGCCTC 
CAGCTGCAGG AGGATCTGAT GAAAGTCCTC AATGAGTTGT ACACGGTGAT GAAGACTTAT 
CACATGTACA ACATGGACAG CATCAACGCA GAGAGCAAGC TGAAGGAGGC AGAGAAGCAG 
GAGGAGAAAC AGATGAGCCG CAGTGTCCGG CATGAGGAAA AACAGACCCC TCGCTCACCT 
GACTCTCTCA CCAACATCAA GATCGAGGAT AAACACGTGC GCCGCAGCTC CGTCAAGAAG 
ATTGAGAAGA TGAAGGAGAA GCGGCAGGCC AAATACACTG AGAATAAGCT GAAAGCTATC 
AAGGCCAGGA ATGAGTATCT TCTGGCTCTG GAGGCCACAA ACAGCTGCGT CTTCAAATAC 
TACATCCATG ATCTGTCTGA CCTCATCGAT TGCTGTGATC TGGGCTACCA TGCCAGCCTG 
AACCGTGCAC TGCGTACCTA CCTCTCGGCT GAGTTTAACG TGGAGACATC CAAACACGGG 
GGCCTGGAGA CCATTGAGAA TGCAGCAGAA AACCTGGAGG CCAACTCAGA CAAACAGCGG 
CTCATGGAGA CCTACAACAA CGTCTTCTGT CCTCCGATGC GCTTCGATTT CCAGTCTCAC 
ATGGGTGACA TGGTTGGACA TTTGTGTGCC CAGCAGCCTG TGCAGGGAGA GCTGATCCAG 
AGATGCCAGC AGCTTCAGTC CCGTCTGTCC ACACTGAATA TAGAGAATGA AGAGGTTAAG 
AAGACCATGG AGGCCACTTT ACAGACCATC CAGGACATGG TGACCATAGA GGACTTCGAT 
GTGACCGACT GCTTCCACCA CAGCAACTCA ATGGAGTCGG TCAAGTCCAC AGTGTCTGAG 
TCTTTCATGA GCAAACCCAG CCTGGCCAAG AGACGAGCCA ACCAGCAGGA GACCGAACAG 
TTCTACTTCA CGAAACTGAA GGAGTTTCTG GAGGGCAGGA ACCTCATCAC CAAACTGCAG 
GCTAAACACG ATCTGATCCA AAAGACTCTG GGAGAAAGTC AGAAGACGGA CTACTGCCTT 
GCCAGCGGAC GGAGGGACTC CACTGTGCGG AAACAGGAGG CCATCCAAAT CATTCCCCTG 
ATGGTGGAGA GTTGTATTCG CTTCATCAGT CGACACGGTC TCCATCATGA GGGGATTTTC 
AGGGTGTCTG GCTCCCAGGT GGAGGTCAAT GACATTAAAA ACGCCTTTGA AAGAGGTGAG 
GATCCGCTGG CTGGAGATCA GAATGATCAC GATATGGACT CCATCGCTGG AGTGCTGAAG 
CTCTACTTCA GAGGCCTGGA AAACGCCCTG TTTCCTAAGG AAGTCTTCCA TGACCTCATG 
TCCTGTGTCT CCATTGAGAG CCTCCAGGAA AGAGCTGTGC ACATCCGAAA AGTCCTGCTG 
TCGCTGCCCA GCAACATCCT GGTTATCATG AGATACCTGT TTGCTTTTCT CAATCATCTG 
TCTCAGTACA GTGACGACAA CATGATGGAC CCCTACAACC TGGCTATCTG CTTCGGCCCC 
ACACTCATGT CTGTTCCTGA GGGCCACGAT CAAGTCTCCT GCCAGGCACA TGTGAACGAG 
CTCATCAAAA CCATTATTAT CCACCACGAG AGCATCTTTC CAGGGCCCCG TGAGCTGGAG 
GGCCCCGTCT ACGACCGTGG AGGAGCCCCT GAGGAATACT GTGATAGTCC ACACATTGAG 
CCCCCTCTAG TGGACGAACC TGCTCCTGAC ACGGTCTCTG TAATCCACAA CAGTGATGAC 
GTAAAGTCAG GGCCATTGAC CGTTTCAGAG TCGGACCCCA TCGAGGCCAT CGCTCGCTTC 
GATTACTCTG GACGCACCAA CCGCGAGCTG AGCTTCAAAA AGGGGGCGTC TCTGCTGCTG 
TACAGCCGCG CATCTGATGA CTGGTGGGAG GGACGACACA ACGGCACTGA GGGTCTCGTG 
CCGCACCAGT ACATAGTAGT GCAGGACATG CCGGATGGAT ATGCAGGCAG AGGAAGTCCT 
AAAGCTGACC TCGAAGGATC CCATGATTCA GTGGAAGAGA AGGTTTCCAC GAGAGCCAGT 
GCCAGCTCAC CCACTGGAGG TCATGTGGCT GATATCTATC TAGCCAACCT CAACAAGTTG 
AGGAAGCGTC CAGAAGCGAC GAGCATCCGG AGAACCATCC GGCCTGTGGA GGAGGGCTCA 
TCAGGAGCAG CAGGGGGCTT AAAGACCTCC TCTATGCCTG CTGGAGGACT GGCCAAGGAC 
AGCTCAGACA AGCGTCCTGT CAGCGCTCAC AGCGTCCTGA ACTCCATCAC CAGACACTCG 
TCTCTCAAGA CTAAAGTAGA GGGTCCTCAA GTTCGCAAAT CCACACCTAC TGGACGATCC 
AAGAGCTTCA GCAACCACAG ACCCCTCGAC CCAGAGGTGA TCGCGCAGGT GGAGCACAGC 
TCACAGGACA TCGAGGCCAC TATGAACACG GCTCTGAGTG AGCTGCGAGA GCTTGAGAGG 
CAGAGCAACG TCAAACATGC TCCAGACGTG GTTCTCGACA CACTGGAGCA GCTCAAAAGT 
GGCGGGACAT CTGAACCATC CAGCCCTCTC CATTCCCGCC TGCTCCGCGA AGCTGAATCC 
TCTCAGCATC CTCTGCAGCG CAGCGCCAGT TCAGCCAGCG ACATGCCCTC CACTTTCCGA 
CCCAGCAAAA CCACAGGCCC CAAGAGCCCT CTGTCCTCCA TGACCACCGC TTCTGGCTCC 
ACATTCAGAG ATAACAAACC TCCAGCCACC AGACCCAAGC CTGTGGTGTT CCCCAAAAGC 
AGTTCAGCCG GAGGAAGTCC GGCTATGGGA TCTCCAACCA CCACCATCCC ACCAACACCA 
CCACCTCCAC CACCACCCAC CGACAAATCC TGCCCCGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dissection of Glomerular Transcriptional Profile in Patients with Diabetic Nephropathy: SRGAP2a Protects Podocyte Structure and Function.
Diabetes(2017 Dec)
Pan Y,Jiang S,Hou Q,Qiu D,Shi J,Wang L,Chen Z,Zhang M,Duan A,Qin W,Zen K,Liu Z

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

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SRGAP2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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