Srgap2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Srgap2
Entrez Gene ID	14270
Full Name	SLIT-ROBO Rho GTPase activating protein 2
Synonyms	9930124L22Rik,AI448945,FBP2,Fnbp2,srGAP3
General protein information	Preferred Names SLIT-ROBO Rho GTPase activating protein 2 Names SLIT-ROBO Rho GTPase-activating protein 2 FBP-27 formin binding protein 2 formin-binding protein 27
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 E4

mRNA and Protein(s)

mRNA	Protein	Name
XM_006529134.3	XP_006529197.1	SLIT-ROBO Rho GTPase-activating protein 2 isoform X1
XM_011247923.3	XP_011246225.1	SLIT-ROBO Rho GTPase-activating protein 2 isoform X1
NM_001081011.2	NP_001074480.2	SLIT-ROBO Rho GTPase-activating protein 2
XM_011247923.2	XP_011246225.1	SLIT-ROBO Rho GTPase-activating protein 2 isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SRGAP2	XP_417972.3
Homo sapiens (human)	SRGAP2	NP_056141.2
	SRGAP2	XP_001089021.1
Pan troglodytes (chimpanzee)	SRGAP2	XP_001163967.1
Canis lupus familiaris (dog)	SRGAP2	XP_005640863.1
Mus musculus (house mouse)	Srgap2	NP_001074480.2
Danio rerio (zebrafish)	srgap2a	NP_001041700.1
Xenopus tropicalis (tropical clawed frog)	srgap2	XP_002940554.1
Bos taurus (cattle)	SRGAP2	NP_001192794.1
Rattus norvegicus (Norway rat)	Srgap2	NP_001128430.1

Gene Ontology
Bibliography

Related articles in PubMed

Dissection of Glomerular Transcriptional Profile in Patients with Diabetic Nephropathy: SRGAP2a Protects Podocyte Structure and Function.
Pan Y, Jiang S, Hou Q, Qiu D, Shi J, Wang L, Chen Z, Zhang M, Duan A, Qin W, Zen K, Liu Z
Diabetes(2017 Dec)

Slit-Robo GTPase-Activating Protein 2 as a metastasis suppressor in osteosarcoma.
Marko TA, Shamsan GA, Edwards EN, Hazelton PE, Rathe SK, Cornax I, Overn PR, Varshney J, Diessner BJ, Moriarity BS, O'Sullivan MG, Odde DJ, Largaespada DA
Scientific reports639059(2016 Dec)

SRGAP2 and Its Human-Specific Paralog Co-Regulate the Development of Excitatory and Inhibitory Synapses.
Fossati M, Pizzarelli R, Schmidt ER, Kupferman JV, Stroebel D, Polleux F, Charrier C
Neuron91(2)356-69(2016 Jul)

The inverse F-BAR domain protein srGAP2 acts through srGAP3 to modulate neuronal differentiation and neurite outgrowth of mouse neuroblastoma cells.
Ma Y, Mi YJ, Dai YK, Fu HL, Cui DX, Jin WL
PloS one8(3)e57865(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The inverse F-BAR domain protein srGAP2 acts through srGAP3 to modulate neuronal differentiation and neurite outgrowth of mouse neuroblastoma cells.
Title: The inverse F-BAR domain protein srGAP2 acts through srGAP3 to modulate neuronal differentiation and neurite outgrowth of mouse neuroblastoma cells.

Study reports that srGAP2 negatively regulates neuronal migration and induces neurite outgrowth and branching through the ability of its F-BAR domain to induce filopodia-like membrane protrusions resembling those induced by I-BAR domains.
Title: The F-BAR domain of srGAP2 induces membrane protrusions required for neuronal migration and morphogenesis.

Data show that srGAP2 is expressed in zones of neuronal differentiation in many different tissues of the central nervous system.
Title: Dynamic expression of the Slit-Robo GTPase activating protein genes during development of the murine nervous system.

The following Srgap2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Srgap2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu67765	XM_011247923.3 Latest version!	Mus musculus SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu21052	NM_001081011.2 Latest version!	Mus musculus SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu21052	XM_006529134.3 Latest version!	Mus musculus SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu67765	XM_011247923.2	Mus musculus SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Srgap2 ( XM_011247923.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246225.1 Mus musculus SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu67765
Clone ID Related Accession (Same CDS sequence)	XM_011247923.3 , XM_011247923.2
Accession Version	XM_011247923.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	SLIT-ROBO Rho GTPase-activating protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247923.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCTC CAGCCAAATT CAAAAAGGAT AAGGAGATCA TCGCAGAGTA CGATACTCAG 
GTCAAAGAGA TTAGGGCTCA GCTCACAGAG CAGATGAAAT GCCTAGATCA GCAATGTGAG 
CTCCGGGTGC AGCTGTTGCA GGACCTGCAG GACTTCTTCC GCAAGAAGGC TGAGATTGAA 
ATGGACTACT CTCGAAACCT GGAGAAGCTA GCCGAGCGCT TTCTAGCCAA GACACGAAGC 
ACCAAGGACC AGCAATTTAA GAAGGACCAG AATGTTCTTT CTCCAGTCAA CTGCTGGAAC 
CTCCTCTTAA ACCAAGTGAA GCGGGAGAGC AGGGATCATA CCACCCTGAG TGACATCTAC 
CTGAATAATA TAATTCCTCG ATTTGTTCAA GTCAGCGAGG ACTCAGGAAG ACTCTTTAAA 
AAGAGTAAAG AAGTTGGCCA GCAACTCCAA GATGATTTGA TGAAAGTCCT GAACGAGCTT 
TACTCGGTCA TGAAGACATA TCACATGTAC AATGCCGACA GCATCAGTGC TCAGAGCAAA 
CTGAAGGAAG CAGAGAAACA AGAAGAGAAG CAAATTGGCA AATCAGTAAA GCAAGAGGAC 
CGGCAGACCC CTCGCTCCCC TGACTCCACA GCCAATGTCC GCATTGAGGA GAAGCATGTC 
CGGAGGAGCT CAGTGAAGAA GATTGAGAAG ATGAAGGAGA AGCGACAAGC CAAGTACACA 
GAAAATAAGC TGAAGGCCAT TAAAGCCCGG AATGAGTATT TATTGGCTTT GGAGGCAACC 
AATGCATCTG TCTTCAAGTA CTATATCCAT GACCTGTCTG ATATTATTGA TCAATGTTGT 
GACCTAGGCT ACCATGCTAG CCTGAACCGG GCTCTACGCA CTTTCCTATC TGCTGAATTA 
AATCTGGAAC AGTCAAAACA TGAAGGTCTG GATGCTATTG AAAATGCAGT AGAAAACCTA 
GATGCCACCA GTGACAAGCA ACGGCTCATG GAGATGTACA ACAATGTTTT TTGCCCCCCT 
ATGAAATTTG AATTCCAGCC CCACATGGGA GATATGGCCT CTCAACTCTG TGCCCAGCAG 
CCTGTCCAGA GTGAGCTGGT GCAGAGATGC CAACAGCTGC AGTCTCGCTT ATCCACTTTG 
AAGATTGAGA ATGAAGAGGT GAAAAAGACA ATGGAGGCCA CCCTGCAGAC CATTCAAGAC 
ATTGTGACTG TTGAGGATTT TGATGTATCT GACTGCTTCC AGTATAGCAA TTCCATGGAG 
TCTGTCAAAT CAACTGTCTC TGAAACATTC ATGAGCAAGC CCAGCATTGC TAAGAGGAGA 
GCGAACCAGC AAGAGACGGA ACAGTTTTAT TTCACGAAAA TGAAGGAGTA CTTGGAGGGT 
AGGAACCTCA TCACAAAGCT ACAAGCCAAG CATGACCTCC TGCAGAAAAC CCTGGGAGAA 
AGTCAGCGGA CAGACTGTAG TCTTGCCAGG CGAAGCTCAA CTGTGAGGAA ACAGGATTCC 
AGCCAAGCAA TTCCTCTGGT GGTAGAAAGC TGCATCCGGT TTATTAGCAG ACATGGCCTA 
CAACATGAGG GAATTTTCCG GGTTTCTGGA TCACAAGTAG AAGTGAACGA CATAAAAAAC 
GCCTTTGAGA GAGGAGAGGA TCCCCTGGCT GGGGACCAGA ATGACCATGA CATGGACTCT 
ATAGCTGGTG TCCTCAAGCT TTACTTCCGG GGCCTGGAAC ACCCGCTCTT CCCTAAAGAC 
ATCTTCCATG ACTTGATTGC CTGTGTCACA ATGGACAACC TGCAAGAGAG AGCTGTGCAT 
ATCCGGAAAG TCCTTCTGGT CCTGCCCAAG CCCACTCTGA TTATCATGAG ATATCTCTTT 
GCCTTCCTCA ATCACTTATC ACAGTTCAGT GAAGAGAACA TGATGGACCC CTACAACCTT 
GCCATCTGCT TCGGGCCCTC ACTGATGTCA GTGCCAGAGG GCCACGACCA GGTGTCCTGT 
CAAGCCCACG TGAATGAGCT GATCAAAACC ATCATCATCC AACATGAGAA CATTTTCCCA 
AACCCCAGGG AGCTGGAGGG TCCCATCTAC AGCAGAGGAG GAAGCATGGA GGATTACTGT 
GACAGCACTC ATGGAGAGAC TACCTCTGCT GAAGACTCCA CCCAGGACGT TACAGCGGAG 
CACCACACAA GCGATGATGA ATGTGAGCCC ATAGAAGCCA TTGCCAAGTT TGACTACGTA 
GGCCGGACAG CCCGAGAACT GTCTTTCAAG AAGGGAGCAT CCCTGCTGCT CTACCAGCGA 
GCTTCTGATG ACTGGTGGGA GGGCCGGCAC AACGGTATAG ATGGACTCAT CCCCCATCAG 
TACATCGTAG TCCAAGACAC CGAGGACGGT GTCGTGGAGA GGTCCAGCCC CAAGTCTGAG 
ATTGAGGTCA TGTCTGAGCC ACCTGAAGAA AAGGTGACAG CCAGAACGGG GGCCAGCTGT 
CCCAGTGGGG GTCATGTAGC TGATATTTAT CTTGCAAACA TCAACAAGCA AAGGAAGCGT 
CCAGAATCTG GGAGCATCAG AAAAGCATTT CGGAGTGACA GCCATGGGCT GGGCAGTTCT 
CTGACTGACT CCTCCTCCCT GGGGGTGGGG GCTAGCTGCC GTCCATCCTC CCAGCCCATC 
ATGAGCCAGA ATCTCCCCAA GGAAGGGCCA GATAAGTGTT CCATCAGCGG CCATGGCAGC 
CTCAACTCTA TCAGCCGCCA CTCATCCTTG AAGAACCGGA TGGACAGTCC GCAGATCCGG 
AAGACCGCTA CGGCAGGAAG GTCAAAAAGT TTCAATAACC ATCGGCCCAT GGACCCTGAA 
GTCATTGCAC AGAAGCCCAA CCCGACATCT AAACATCCAG CTACTGGGGC CACTCCAAGC 
AGTTCTCTCA GACGTCTCTC CCAGCTATAT AATTTTCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246225.1
CDS	679..3600
Translation	MTSPAKFKKDKEIIAEYDTQVKEIRAQLTEQMKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKLAERFLAKTRSTKDQQFKKDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIYLNNIIPRFVQVSEDSGRLFKKSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISAQSKLKEAEKQEEKQIGKSVKQEDRQTPRSPDSTANVRIEEKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNASVFKYYIHDLSDIIDQCCDLGYHASLNRALRTFLSAELNLEQSKHEGLDAIENAVENLDATSDKQRLMEMYNNVFCPPMKFEFQPHMGDMASQLCAQQPVQSELVQRCQQLQSRLSTLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSMESVKSTVSETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKTLGESQRTDCSLARRSSTVRKQDSSQAIPLVVESCIRFISRHGLQHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLEHPLFPKDIFHDLIACVTMDNLQERAVHIRKVLLVLPKPTLIIMRYLFAFLNHLSQFSEENMMDPYNLAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHENIFPNPRELEGPIYSRGGSMEDYCDSTHGETTSAEDSTQDVTAEHHTSDDECEPIEAIAKFDYVGRTARELSFKKGASLLLYQRASDDWWEGRHNGIDGLIPHQYIVVQDTEDGVVERSSPKSEIEVMSEPPEEKVTARTGASCPSGGHVADIYLANINKQRKRPESGSIRKAFRSDSHGLGSSLTDSSSLGVGASCRPSSQPIMSQNLPKEGPDKCSISGHGSLNSISRHSSLKNRMDSPQIRKTATAGRSKSFNNHRPMDPEVIAQKPNPTSKHPATGATPSSSLRRLSQLYNFH

Target ORF information:

RefSeq Version	XM_011247923.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247923.2

ORF Insert Sequence:

ATGACGTCTC CAGCCAAATT CAAAAAGGAT AAGGAGATCA TCGCAGAGTA CGATACTCAG 
GTCAAAGAGA TTAGGGCTCA GCTCACAGAG CAGATGAAAT GCCTAGATCA GCAATGTGAG 
CTCCGGGTGC AGCTGTTGCA GGACCTGCAG GACTTCTTCC GCAAGAAGGC TGAGATTGAA 
ATGGACTACT CTCGAAACCT GGAGAAGCTA GCCGAGCGCT TTCTAGCCAA GACACGAAGC 
ACCAAGGACC AGCAATTTAA GAAGGACCAG AATGTTCTTT CTCCAGTCAA CTGCTGGAAC 
CTCCTCTTAA ACCAAGTGAA GCGGGAGAGC AGGGATCATA CCACCCTGAG TGACATCTAC 
CTGAATAATA TAATTCCTCG ATTTGTTCAA GTCAGCGAGG ACTCAGGAAG ACTCTTTAAA 
AAGAGTAAAG AAGTTGGCCA GCAACTCCAA GATGATTTGA TGAAAGTCCT GAACGAGCTT 
TACTCGGTCA TGAAGACATA TCACATGTAC AATGCCGACA GCATCAGTGC TCAGAGCAAA 
CTGAAGGAAG CAGAGAAACA AGAAGAGAAG CAAATTGGCA AATCAGTAAA GCAAGAGGAC 
CGGCAGACCC CTCGCTCCCC TGACTCCACA GCCAATGTCC GCATTGAGGA GAAGCATGTC 
CGGAGGAGCT CAGTGAAGAA GATTGAGAAG ATGAAGGAGA AGCGACAAGC CAAGTACACA 
GAAAATAAGC TGAAGGCCAT TAAAGCCCGG AATGAGTATT TATTGGCTTT GGAGGCAACC 
AATGCATCTG TCTTCAAGTA CTATATCCAT GACCTGTCTG ATATTATTGA TCAATGTTGT 
GACCTAGGCT ACCATGCTAG CCTGAACCGG GCTCTACGCA CTTTCCTATC TGCTGAATTA 
AATCTGGAAC AGTCAAAACA TGAAGGTCTG GATGCTATTG AAAATGCAGT AGAAAACCTA 
GATGCCACCA GTGACAAGCA ACGGCTCATG GAGATGTACA ACAATGTTTT TTGCCCCCCT 
ATGAAATTTG AATTCCAGCC CCACATGGGA GATATGGCCT CTCAACTCTG TGCCCAGCAG 
CCTGTCCAGA GTGAGCTGGT GCAGAGATGC CAACAGCTGC AGTCTCGCTT ATCCACTTTG 
AAGATTGAGA ATGAAGAGGT GAAAAAGACA ATGGAGGCCA CCCTGCAGAC CATTCAAGAC 
ATTGTGACTG TTGAGGATTT TGATGTATCT GACTGCTTCC AGTATAGCAA TTCCATGGAG 
TCTGTCAAAT CAACTGTCTC TGAAACATTC ATGAGCAAGC CCAGCATTGC TAAGAGGAGA 
GCGAACCAGC AAGAGACGGA ACAGTTTTAT TTCACGAAAA TGAAGGAGTA CTTGGAGGGT 
AGGAACCTCA TCACAAAGCT ACAAGCCAAG CATGACCTCC TGCAGAAAAC CCTGGGAGAA 
AGTCAGCGGA CAGACTGTAG TCTTGCCAGG CGAAGCTCAA CTGTGAGGAA ACAGGATTCC 
AGCCAAGCAA TTCCTCTGGT GGTAGAAAGC TGCATCCGGT TTATTAGCAG ACATGGCCTA 
CAACATGAGG GAATTTTCCG GGTTTCTGGA TCACAAGTAG AAGTGAACGA CATAAAAAAC 
GCCTTTGAGA GAGGAGAGGA TCCCCTGGCT GGGGACCAGA ATGACCATGA CATGGACTCT 
ATAGCTGGTG TCCTCAAGCT TTACTTCCGG GGCCTGGAAC ACCCGCTCTT CCCTAAAGAC 
ATCTTCCATG ACTTGATTGC CTGTGTCACA ATGGACAACC TGCAAGAGAG AGCTGTGCAT 
ATCCGGAAAG TCCTTCTGGT CCTGCCCAAG CCCACTCTGA TTATCATGAG ATATCTCTTT 
GCCTTCCTCA ATCACTTATC ACAGTTCAGT GAAGAGAACA TGATGGACCC CTACAACCTT 
GCCATCTGCT TCGGGCCCTC ACTGATGTCA GTGCCAGAGG GCCACGACCA GGTGTCCTGT 
CAAGCCCACG TGAATGAGCT GATCAAAACC ATCATCATCC AACATGAGAA CATTTTCCCA 
AACCCCAGGG AGCTGGAGGG TCCCATCTAC AGCAGAGGAG GAAGCATGGA GGATTACTGT 
GACAGCACTC ATGGAGAGAC TACCTCTGCT GAAGACTCCA CCCAGGACGT TACAGCGGAG 
CACCACACAA GCGATGATGA ATGTGAGCCC ATAGAAGCCA TTGCCAAGTT TGACTACGTA 
GGCCGGACAG CCCGAGAACT GTCTTTCAAG AAGGGAGCAT CCCTGCTGCT CTACCAGCGA 
GCTTCTGATG ACTGGTGGGA GGGCCGGCAC AACGGTATAG ATGGACTCAT CCCCCATCAG 
TACATCGTAG TCCAAGACAC CGAGGACGGT GTCGTGGAGA GGTCCAGCCC CAAGTCTGAG 
ATTGAGGTCA TGTCTGAGCC ACCTGAAGAA AAGGTGACAG CCAGAACGGG GGCCAGCTGT 
CCCAGTGGGG GTCATGTAGC TGATATTTAT CTTGCAAACA TCAACAAGCA AAGGAAGCGT 
CCAGAATCTG GGAGCATCAG AAAAGCATTT CGGAGTGACA GCCATGGGCT GGGCAGTTCT 
CTGACTGACT CCTCCTCCCT GGGGGTGGGG GCTAGCTGCC GTCCATCCTC CCAGCCCATC 
ATGAGCCAGA ATCTCCCCAA GGAAGGGCCA GATAAGTGTT CCATCAGCGG CCATGGCAGC 
CTCAACTCTA TCAGCCGCCA CTCATCCTTG AAGAACCGGA TGGACAGTCC GCAGATCCGG 
AAGACCGCTA CGGCAGGAAG GTCAAAAAGT TTCAATAACC ATCGGCCCAT GGACCCTGAA 
GTCATTGCAC AGAAGCCCAA CCCGACATCT AAACATCCAG CTACTGGGGC CACTCCAAGC 
AGTTCTCTCA GACGTCTCTC CCAGCTATAT AATTTTCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Srgap2 ( XM_011247923.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246225.1 Mus musculus SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67765
Clone ID Related Accession (Same CDS sequence)	XM_011247923.3 , XM_011247923.2
Accession Version	XM_011247923.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	SLIT-ROBO Rho GTPase-activating protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247923.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCTC CAGCCAAATT CAAAAAGGAT AAGGAGATCA TCGCAGAGTA CGATACTCAG 
GTCAAAGAGA TTAGGGCTCA GCTCACAGAG CAGATGAAAT GCCTAGATCA GCAATGTGAG 
CTCCGGGTGC AGCTGTTGCA GGACCTGCAG GACTTCTTCC GCAAGAAGGC TGAGATTGAA 
ATGGACTACT CTCGAAACCT GGAGAAGCTA GCCGAGCGCT TTCTAGCCAA GACACGAAGC 
ACCAAGGACC AGCAATTTAA GAAGGACCAG AATGTTCTTT CTCCAGTCAA CTGCTGGAAC 
CTCCTCTTAA ACCAAGTGAA GCGGGAGAGC AGGGATCATA CCACCCTGAG TGACATCTAC 
CTGAATAATA TAATTCCTCG ATTTGTTCAA GTCAGCGAGG ACTCAGGAAG ACTCTTTAAA 
AAGAGTAAAG AAGTTGGCCA GCAACTCCAA GATGATTTGA TGAAAGTCCT GAACGAGCTT 
TACTCGGTCA TGAAGACATA TCACATGTAC AATGCCGACA GCATCAGTGC TCAGAGCAAA 
CTGAAGGAAG CAGAGAAACA AGAAGAGAAG CAAATTGGCA AATCAGTAAA GCAAGAGGAC 
CGGCAGACCC CTCGCTCCCC TGACTCCACA GCCAATGTCC GCATTGAGGA GAAGCATGTC 
CGGAGGAGCT CAGTGAAGAA GATTGAGAAG ATGAAGGAGA AGCGACAAGC CAAGTACACA 
GAAAATAAGC TGAAGGCCAT TAAAGCCCGG AATGAGTATT TATTGGCTTT GGAGGCAACC 
AATGCATCTG TCTTCAAGTA CTATATCCAT GACCTGTCTG ATATTATTGA TCAATGTTGT 
GACCTAGGCT ACCATGCTAG CCTGAACCGG GCTCTACGCA CTTTCCTATC TGCTGAATTA 
AATCTGGAAC AGTCAAAACA TGAAGGTCTG GATGCTATTG AAAATGCAGT AGAAAACCTA 
GATGCCACCA GTGACAAGCA ACGGCTCATG GAGATGTACA ACAATGTTTT TTGCCCCCCT 
ATGAAATTTG AATTCCAGCC CCACATGGGA GATATGGCCT CTCAACTCTG TGCCCAGCAG 
CCTGTCCAGA GTGAGCTGGT GCAGAGATGC CAACAGCTGC AGTCTCGCTT ATCCACTTTG 
AAGATTGAGA ATGAAGAGGT GAAAAAGACA ATGGAGGCCA CCCTGCAGAC CATTCAAGAC 
ATTGTGACTG TTGAGGATTT TGATGTATCT GACTGCTTCC AGTATAGCAA TTCCATGGAG 
TCTGTCAAAT CAACTGTCTC TGAAACATTC ATGAGCAAGC CCAGCATTGC TAAGAGGAGA 
GCGAACCAGC AAGAGACGGA ACAGTTTTAT TTCACGAAAA TGAAGGAGTA CTTGGAGGGT 
AGGAACCTCA TCACAAAGCT ACAAGCCAAG CATGACCTCC TGCAGAAAAC CCTGGGAGAA 
AGTCAGCGGA CAGACTGTAG TCTTGCCAGG CGAAGCTCAA CTGTGAGGAA ACAGGATTCC 
AGCCAAGCAA TTCCTCTGGT GGTAGAAAGC TGCATCCGGT TTATTAGCAG ACATGGCCTA 
CAACATGAGG GAATTTTCCG GGTTTCTGGA TCACAAGTAG AAGTGAACGA CATAAAAAAC 
GCCTTTGAGA GAGGAGAGGA TCCCCTGGCT GGGGACCAGA ATGACCATGA CATGGACTCT 
ATAGCTGGTG TCCTCAAGCT TTACTTCCGG GGCCTGGAAC ACCCGCTCTT CCCTAAAGAC 
ATCTTCCATG ACTTGATTGC CTGTGTCACA ATGGACAACC TGCAAGAGAG AGCTGTGCAT 
ATCCGGAAAG TCCTTCTGGT CCTGCCCAAG CCCACTCTGA TTATCATGAG ATATCTCTTT 
GCCTTCCTCA ATCACTTATC ACAGTTCAGT GAAGAGAACA TGATGGACCC CTACAACCTT 
GCCATCTGCT TCGGGCCCTC ACTGATGTCA GTGCCAGAGG GCCACGACCA GGTGTCCTGT 
CAAGCCCACG TGAATGAGCT GATCAAAACC ATCATCATCC AACATGAGAA CATTTTCCCA 
AACCCCAGGG AGCTGGAGGG TCCCATCTAC AGCAGAGGAG GAAGCATGGA GGATTACTGT 
GACAGCACTC ATGGAGAGAC TACCTCTGCT GAAGACTCCA CCCAGGACGT TACAGCGGAG 
CACCACACAA GCGATGATGA ATGTGAGCCC ATAGAAGCCA TTGCCAAGTT TGACTACGTA 
GGCCGGACAG CCCGAGAACT GTCTTTCAAG AAGGGAGCAT CCCTGCTGCT CTACCAGCGA 
GCTTCTGATG ACTGGTGGGA GGGCCGGCAC AACGGTATAG ATGGACTCAT CCCCCATCAG 
TACATCGTAG TCCAAGACAC CGAGGACGGT GTCGTGGAGA GGTCCAGCCC CAAGTCTGAG 
ATTGAGGTCA TGTCTGAGCC ACCTGAAGAA AAGGTGACAG CCAGAACGGG GGCCAGCTGT 
CCCAGTGGGG GTCATGTAGC TGATATTTAT CTTGCAAACA TCAACAAGCA AAGGAAGCGT 
CCAGAATCTG GGAGCATCAG AAAAGCATTT CGGAGTGACA GCCATGGGCT GGGCAGTTCT 
CTGACTGACT CCTCCTCCCT GGGGGTGGGG GCTAGCTGCC GTCCATCCTC CCAGCCCATC 
ATGAGCCAGA ATCTCCCCAA GGAAGGGCCA GATAAGTGTT CCATCAGCGG CCATGGCAGC 
CTCAACTCTA TCAGCCGCCA CTCATCCTTG AAGAACCGGA TGGACAGTCC GCAGATCCGG 
AAGACCGCTA CGGCAGGAAG GTCAAAAAGT TTCAATAACC ATCGGCCCAT GGACCCTGAA 
GTCATTGCAC AGAAGCCCAA CCCGACATCT AAACATCCAG CTACTGGGGC CACTCCAAGC 
AGTTCTCTCA GACGTCTCTC CCAGCTATAT AATTTTCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246225.1
CDS	695..3616
Translation	MTSPAKFKKDKEIIAEYDTQVKEIRAQLTEQMKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKLAERFLAKTRSTKDQQFKKDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIYLNNIIPRFVQVSEDSGRLFKKSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISAQSKLKEAEKQEEKQIGKSVKQEDRQTPRSPDSTANVRIEEKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNASVFKYYIHDLSDIIDQCCDLGYHASLNRALRTFLSAELNLEQSKHEGLDAIENAVENLDATSDKQRLMEMYNNVFCPPMKFEFQPHMGDMASQLCAQQPVQSELVQRCQQLQSRLSTLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSMESVKSTVSETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKTLGESQRTDCSLARRSSTVRKQDSSQAIPLVVESCIRFISRHGLQHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLEHPLFPKDIFHDLIACVTMDNLQERAVHIRKVLLVLPKPTLIIMRYLFAFLNHLSQFSEENMMDPYNLAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHENIFPNPRELEGPIYSRGGSMEDYCDSTHGETTSAEDSTQDVTAEHHTSDDECEPIEAIAKFDYVGRTARELSFKKGASLLLYQRASDDWWEGRHNGIDGLIPHQYIVVQDTEDGVVERSSPKSEIEVMSEPPEEKVTARTGASCPSGGHVADIYLANINKQRKRPESGSIRKAFRSDSHGLGSSLTDSSSLGVGASCRPSSQPIMSQNLPKEGPDKCSISGHGSLNSISRHSSLKNRMDSPQIRKTATAGRSKSFNNHRPMDPEVIAQKPNPTSKHPATGATPSSSLRRLSQLYNFH

Target ORF information:

RefSeq Version	XM_011247923.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247923.3

ORF Insert Sequence:

ATGACGTCTC CAGCCAAATT CAAAAAGGAT AAGGAGATCA TCGCAGAGTA CGATACTCAG 
GTCAAAGAGA TTAGGGCTCA GCTCACAGAG CAGATGAAAT GCCTAGATCA GCAATGTGAG 
CTCCGGGTGC AGCTGTTGCA GGACCTGCAG GACTTCTTCC GCAAGAAGGC TGAGATTGAA 
ATGGACTACT CTCGAAACCT GGAGAAGCTA GCCGAGCGCT TTCTAGCCAA GACACGAAGC 
ACCAAGGACC AGCAATTTAA GAAGGACCAG AATGTTCTTT CTCCAGTCAA CTGCTGGAAC 
CTCCTCTTAA ACCAAGTGAA GCGGGAGAGC AGGGATCATA CCACCCTGAG TGACATCTAC 
CTGAATAATA TAATTCCTCG ATTTGTTCAA GTCAGCGAGG ACTCAGGAAG ACTCTTTAAA 
AAGAGTAAAG AAGTTGGCCA GCAACTCCAA GATGATTTGA TGAAAGTCCT GAACGAGCTT 
TACTCGGTCA TGAAGACATA TCACATGTAC AATGCCGACA GCATCAGTGC TCAGAGCAAA 
CTGAAGGAAG CAGAGAAACA AGAAGAGAAG CAAATTGGCA AATCAGTAAA GCAAGAGGAC 
CGGCAGACCC CTCGCTCCCC TGACTCCACA GCCAATGTCC GCATTGAGGA GAAGCATGTC 
CGGAGGAGCT CAGTGAAGAA GATTGAGAAG ATGAAGGAGA AGCGACAAGC CAAGTACACA 
GAAAATAAGC TGAAGGCCAT TAAAGCCCGG AATGAGTATT TATTGGCTTT GGAGGCAACC 
AATGCATCTG TCTTCAAGTA CTATATCCAT GACCTGTCTG ATATTATTGA TCAATGTTGT 
GACCTAGGCT ACCATGCTAG CCTGAACCGG GCTCTACGCA CTTTCCTATC TGCTGAATTA 
AATCTGGAAC AGTCAAAACA TGAAGGTCTG GATGCTATTG AAAATGCAGT AGAAAACCTA 
GATGCCACCA GTGACAAGCA ACGGCTCATG GAGATGTACA ACAATGTTTT TTGCCCCCCT 
ATGAAATTTG AATTCCAGCC CCACATGGGA GATATGGCCT CTCAACTCTG TGCCCAGCAG 
CCTGTCCAGA GTGAGCTGGT GCAGAGATGC CAACAGCTGC AGTCTCGCTT ATCCACTTTG 
AAGATTGAGA ATGAAGAGGT GAAAAAGACA ATGGAGGCCA CCCTGCAGAC CATTCAAGAC 
ATTGTGACTG TTGAGGATTT TGATGTATCT GACTGCTTCC AGTATAGCAA TTCCATGGAG 
TCTGTCAAAT CAACTGTCTC TGAAACATTC ATGAGCAAGC CCAGCATTGC TAAGAGGAGA 
GCGAACCAGC AAGAGACGGA ACAGTTTTAT TTCACGAAAA TGAAGGAGTA CTTGGAGGGT 
AGGAACCTCA TCACAAAGCT ACAAGCCAAG CATGACCTCC TGCAGAAAAC CCTGGGAGAA 
AGTCAGCGGA CAGACTGTAG TCTTGCCAGG CGAAGCTCAA CTGTGAGGAA ACAGGATTCC 
AGCCAAGCAA TTCCTCTGGT GGTAGAAAGC TGCATCCGGT TTATTAGCAG ACATGGCCTA 
CAACATGAGG GAATTTTCCG GGTTTCTGGA TCACAAGTAG AAGTGAACGA CATAAAAAAC 
GCCTTTGAGA GAGGAGAGGA TCCCCTGGCT GGGGACCAGA ATGACCATGA CATGGACTCT 
ATAGCTGGTG TCCTCAAGCT TTACTTCCGG GGCCTGGAAC ACCCGCTCTT CCCTAAAGAC 
ATCTTCCATG ACTTGATTGC CTGTGTCACA ATGGACAACC TGCAAGAGAG AGCTGTGCAT 
ATCCGGAAAG TCCTTCTGGT CCTGCCCAAG CCCACTCTGA TTATCATGAG ATATCTCTTT 
GCCTTCCTCA ATCACTTATC ACAGTTCAGT GAAGAGAACA TGATGGACCC CTACAACCTT 
GCCATCTGCT TCGGGCCCTC ACTGATGTCA GTGCCAGAGG GCCACGACCA GGTGTCCTGT 
CAAGCCCACG TGAATGAGCT GATCAAAACC ATCATCATCC AACATGAGAA CATTTTCCCA 
AACCCCAGGG AGCTGGAGGG TCCCATCTAC AGCAGAGGAG GAAGCATGGA GGATTACTGT 
GACAGCACTC ATGGAGAGAC TACCTCTGCT GAAGACTCCA CCCAGGACGT TACAGCGGAG 
CACCACACAA GCGATGATGA ATGTGAGCCC ATAGAAGCCA TTGCCAAGTT TGACTACGTA 
GGCCGGACAG CCCGAGAACT GTCTTTCAAG AAGGGAGCAT CCCTGCTGCT CTACCAGCGA 
GCTTCTGATG ACTGGTGGGA GGGCCGGCAC AACGGTATAG ATGGACTCAT CCCCCATCAG 
TACATCGTAG TCCAAGACAC CGAGGACGGT GTCGTGGAGA GGTCCAGCCC CAAGTCTGAG 
ATTGAGGTCA TGTCTGAGCC ACCTGAAGAA AAGGTGACAG CCAGAACGGG GGCCAGCTGT 
CCCAGTGGGG GTCATGTAGC TGATATTTAT CTTGCAAACA TCAACAAGCA AAGGAAGCGT 
CCAGAATCTG GGAGCATCAG AAAAGCATTT CGGAGTGACA GCCATGGGCT GGGCAGTTCT 
CTGACTGACT CCTCCTCCCT GGGGGTGGGG GCTAGCTGCC GTCCATCCTC CCAGCCCATC 
ATGAGCCAGA ATCTCCCCAA GGAAGGGCCA GATAAGTGTT CCATCAGCGG CCATGGCAGC 
CTCAACTCTA TCAGCCGCCA CTCATCCTTG AAGAACCGGA TGGACAGTCC GCAGATCCGG 
AAGACCGCTA CGGCAGGAAG GTCAAAAAGT TTCAATAACC ATCGGCCCAT GGACCCTGAA 
GTCATTGCAC AGAAGCCCAA CCCGACATCT AAACATCCAG CTACTGGGGC CACTCCAAGC 
AGTTCTCTCA GACGTCTCTC CCAGCTATAT AATTTTCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Srgap2 ( NM_001081011.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074480.2 Mus musculus SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), mRNA.

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CloneID	OMu21052
Clone ID Related Accession (Same CDS sequence)	XM_006529134.3 , NM_001081011.2
Accession Version	NM_001081011.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	SLIT-ROBO Rho GTPase-activating protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY779945.1, AK084412.1, CF164437.1, BC030457.1 and AC165436.5. On Oct 6, 2007 this sequence version replaced NM_001081011.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC151081.1, SRR1660813.53198.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGACGTCTC CAGCCAAATT CAAAAAGGAT AAGGAGATCA TCGCAGAGTA CGATACTCAG 
GTCAAAGAGA TTAGGGCTCA GCTCACAGAG CAGATGAAAT GCCTAGATCA GCAATGTGAG 
CTCCGGGTGC AGCTGTTGCA GGACCTGCAG GACTTCTTCC GCAAGAAGGC TGAGATTGAA 
ATGGACTACT CTCGAAACCT GGAGAAGCTA GCCGAGCGCT TTCTAGCCAA GACACGAAGC 
ACCAAGGACC AGCAATTTAA GAAGGACCAG AATGTTCTTT CTCCAGTCAA CTGCTGGAAC 
CTCCTCTTAA ACCAAGTGAA GCGGGAGAGC AGGGATCATA CCACCCTGAG TGACATCTAC 
CTGAATAATA TAATTCCTCG ATTTGTTCAA GTCAGCGAGG ACTCAGGAAG ACTCTTTAAA 
AAGAGTAAAG AAGTTGGCCA GCAACTCCAA GATGATTTGA TGAAAGTCCT GAACGAGCTT 
TACTCGGTCA TGAAGACATA TCACATGTAC AATGCCGACA GCATCAGTGC TCAGAGCAAA 
CTGAAGGAAG CAGAGAAACA AGAAGAGAAG CAAATTGGCA AATCAGTAAA GCAAGAGGAC 
CGGCAGACCC CTCGCTCCCC TGACTCCACA GCCAATGTCC GCATTGAGGA GAAGCATGTC 
CGGAGGAGCT CAGTGAAGAA GATTGAGAAG ATGAAGGAGA AGCGACAAGC CAAGTACACA 
GAAAATAAGC TGAAGGCCAT TAAAGCCCGG AATGAGTATT TATTGGCTTT GGAGGCAACC 
AATGCATCTG TCTTCAAGTA CTATATCCAT GACCTGTCTG ATATTATTGA TCAATGTTGT 
GACCTAGGCT ACCATGCTAG CCTGAACCGG GCTCTACGCA CTTTCCTATC TGCTGAATTA 
AATCTGGAAC AGTCAAAACA TGAAGGTCTG GATGCTATTG AAAATGCAGT AGAAAACCTA 
GATGCCACCA GTGACAAGCA ACGGCTCATG GAGATGTACA ACAATGTTTT TTGCCCCCCT 
ATGAAATTTG AATTCCAGCC CCACATGGGA GATATGGCCT CTCAACTCTG TGCCCAGCAG 
CCTGTCCAGA GTGAGCTGGT GCAGAGATGC CAACAGCTGC AGTCTCGCTT ATCCACTTTG 
AAGATTGAGA ATGAAGAGGT GAAAAAGACA ATGGAGGCCA CCCTGCAGAC CATTCAAGAC 
ATTGTGACTG TTGAGGATTT TGATGTATCT GACTGCTTCC AGTATAGCAA TTCCATGGAG 
TCTGTCAAAT CAACTGTCTC TGAAACATTC ATGAGCAAGC CCAGCATTGC TAAGAGGAGA 
GCGAACCAGC AAGAGACGGA ACAGTTTTAT TTCACGAAAA TGAAGGAGTA CTTGGAGGGT 
AGGAACCTCA TCACAAAGCT ACAAGCCAAG CATGACCTCC TGCAGAAAAC CCTGGGAGAA 
AGTCAGCGGA CAGACTGTAG TCTTGCCAGG CGAAGCTCAA CTGTGAGGAA ACAGGATTCC 
AGCCAAGCAA TTCCTCTGGT GGTAGAAAGC TGCATCCGGT TTATTAGCAG ACATGGCCTA 
CAACATGAGG GAATTTTCCG GGTTTCTGGA TCACAAGTAG AAGTGAACGA CATAAAAAAC 
GCCTTTGAGA GAGGAGAGGA TCCCCTGGCT GGGGACCAGA ATGACCATGA CATGGACTCT 
ATAGCTGGTG TCCTCAAGCT TTACTTCCGG GGCCTGGAAC ACCCGCTCTT CCCTAAAGAC 
ATCTTCCATG ACTTGATTGC CTGTGTCACA ATGGACAACC TGCAAGAGAG AGCTGTGCAT 
ATCCGGAAAG TCCTTCTGGT CCTGCCCAAG CCCACTCTGA TTATCATGAG ATATCTCTTT 
GCCTTCCTCA ATCACTTATC ACAGTTCAGT GAAGAGAACA TGATGGACCC CTACAACCTT 
GCCATCTGCT TCGGGCCCTC ACTGATGTCA GTGCCAGAGG GCCACGACCA GGTGTCCTGT 
CAAGCCCACG TGAATGAGCT GATCAAAACC ATCATCATCC AACATGAGAA CATTTTCCCA 
AACCCCAGGG AGCTGGAGGG TCCCATCTAC AGCAGAGGAG GAAGCATGGA GGATTACTGT 
GACAGCACTC ATGGAGAGAC TACCTCTGCT GAAGACTCCA CCCAGGACGT TACAGCGGAG 
CACCACACAA GCGATGATGA ATGTGAGCCC ATAGAAGCCA TTGCCAAGTT TGACTACGTA 
GGCCGGACAG CCCGAGAACT GTCTTTCAAG AAGGGAGCAT CCCTGCTGCT CTACCAGCGA 
GCTTCTGATG ACTGGTGGGA GGGCCGGCAC AACGGTATAG ATGGACTCAT CCCCCATCAG 
TACATCGTAG TCCAAGACAC CGAGGACGGT GTCGTGGAGA GGTCCAGCCC CAAGTCTGAG 
ATTGAGGTCA TGTCTGAGCC ACCTGAAGAA AAGGTGACAG CCAGAACGGG GGCCAGCTGT 
CCCAGTGGGG GTCATGTAGC TGATATTTAT CTTGCAAACA TCAACAAGCA AAGGAAGCGT 
CCAGAATCTG GGAGCATCAG AAAAGCATTT CGGAGTGACA GCCATGGGCT GGGCAGTTCT 
CTGACTGACT CCTCCTCCCT GGGGGTGGGG GCTAGCTGCC GTCCATCCTC CCAGCCCATC 
ATGAGCCAGA ATCTCCCCAA GGAAGGGCCA GATAAGTGTT CCATCAGCGG CCATGGCAGC 
CTCAACTCTA TCAGCCGCCA CTCATCCTTG AAGAACCGGA TGGACAGTCC GCAGATCCGG 
AAGACCGCTA CGGCAGGAAG GTCAAAAAGT TTCAATAACC ATCGGCCCAT GGACCCTGAA 
GTCATTGCAC AGGATATTGA AGCAACAATG AACTCTGCCC TGAATGAGCT TCAAGAGCTA 
GAGCGGCAGA GCAGTGCTAA GCACACACCT GATGTGGTTC TGGACACCTT GGAACCACTC 
AAGACCTCCC CGGTGGTAGC CCCCACATCT GAGCCCTCCA GCCCTCTGCA CACCCAGCTC 
CTCAAGGACC CTGAGCCTGC CTTCCAGCGC AGCGCTAGTA CTGCTGGGGA CATTGCCTGC 
GCCTTCCGGC CTGTAAAGTC TGTCAAGATG GCTGCTCCAG TCAAACCACC AGCCACACGG 
CCCAAGCCAA CTGTCTTCCC CAAAACAAAC GCCACTAGCC CTGGTGTCAA TTCATCTGCT 
TCCCCACAGG CCACTGACAA GTCTTGTACT GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074480.2
CDS	719..3934
Translation	MTSPAKFKKDKEIIAEYDTQVKEIRAQLTEQMKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKLAERFLAKTRSTKDQQFKKDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIYLNNIIPRFVQVSEDSGRLFKKSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISAQSKLKEAEKQEEKQIGKSVKQEDRQTPRSPDSTANVRIEEKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNASVFKYYIHDLSDIIDQCCDLGYHASLNRALRTFLSAELNLEQSKHEGLDAIENAVENLDATSDKQRLMEMYNNVFCPPMKFEFQPHMGDMASQLCAQQPVQSELVQRCQQLQSRLSTLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSMESVKSTVSETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKTLGESQRTDCSLARRSSTVRKQDSSQAIPLVVESCIRFISRHGLQHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLEHPLFPKDIFHDLIACVTMDNLQERAVHIRKVLLVLPKPTLIIMRYLFAFLNHLSQFSEENMMDPYNLAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHENIFPNPRELEGPIYSRGGSMEDYCDSTHGETTSAEDSTQDVTAEHHTSDDECEPIEAIAKFDYVGRTARELSFKKGASLLLYQRASDDWWEGRHNGIDGLIPHQYIVVQDTEDGVVERSSPKSEIEVMSEPPEEKVTARTGASCPSGGHVADIYLANINKQRKRPESGSIRKAFRSDSHGLGSSLTDSSSLGVGASCRPSSQPIMSQNLPKEGPDKCSISGHGSLNSISRHSSLKNRMDSPQIRKTATAGRSKSFNNHRPMDPEVIAQDIEATMNSALNELQELERQSSAKHTPDVVLDTLEPLKTSPVVAPTSEPSSPLHTQLLKDPEPAFQRSASTAGDIACAFRPVKSVKMAAPVKPPATRPKPTVFPKTNATSPGVNSSASPQATDKSCTV

Target ORF information:

RefSeq Version	NM_001081011.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081011.2

ORF Insert Sequence:

ATGACGTCTC CAGCCAAATT CAAAAAGGAT AAGGAGATCA TCGCAGAGTA CGATACTCAG 
GTCAAAGAGA TTAGGGCTCA GCTCACAGAG CAGATGAAAT GCCTAGATCA GCAATGTGAG 
CTCCGGGTGC AGCTGTTGCA GGACCTGCAG GACTTCTTCC GCAAGAAGGC TGAGATTGAA 
ATGGACTACT CTCGAAACCT GGAGAAGCTA GCCGAGCGCT TTCTAGCCAA GACACGAAGC 
ACCAAGGACC AGCAATTTAA GAAGGACCAG AATGTTCTTT CTCCAGTCAA CTGCTGGAAC 
CTCCTCTTAA ACCAAGTGAA GCGGGAGAGC AGGGATCATA CCACCCTGAG TGACATCTAC 
CTGAATAATA TAATTCCTCG ATTTGTTCAA GTCAGCGAGG ACTCAGGAAG ACTCTTTAAA 
AAGAGTAAAG AAGTTGGCCA GCAACTCCAA GATGATTTGA TGAAAGTCCT GAACGAGCTT 
TACTCGGTCA TGAAGACATA TCACATGTAC AATGCCGACA GCATCAGTGC TCAGAGCAAA 
CTGAAGGAAG CAGAGAAACA AGAAGAGAAG CAAATTGGCA AATCAGTAAA GCAAGAGGAC 
CGGCAGACCC CTCGCTCCCC TGACTCCACA GCCAATGTCC GCATTGAGGA GAAGCATGTC 
CGGAGGAGCT CAGTGAAGAA GATTGAGAAG ATGAAGGAGA AGCGACAAGC CAAGTACACA 
GAAAATAAGC TGAAGGCCAT TAAAGCCCGG AATGAGTATT TATTGGCTTT GGAGGCAACC 
AATGCATCTG TCTTCAAGTA CTATATCCAT GACCTGTCTG ATATTATTGA TCAATGTTGT 
GACCTAGGCT ACCATGCTAG CCTGAACCGG GCTCTACGCA CTTTCCTATC TGCTGAATTA 
AATCTGGAAC AGTCAAAACA TGAAGGTCTG GATGCTATTG AAAATGCAGT AGAAAACCTA 
GATGCCACCA GTGACAAGCA ACGGCTCATG GAGATGTACA ACAATGTTTT TTGCCCCCCT 
ATGAAATTTG AATTCCAGCC CCACATGGGA GATATGGCCT CTCAACTCTG TGCCCAGCAG 
CCTGTCCAGA GTGAGCTGGT GCAGAGATGC CAACAGCTGC AGTCTCGCTT ATCCACTTTG 
AAGATTGAGA ATGAAGAGGT GAAAAAGACA ATGGAGGCCA CCCTGCAGAC CATTCAAGAC 
ATTGTGACTG TTGAGGATTT TGATGTATCT GACTGCTTCC AGTATAGCAA TTCCATGGAG 
TCTGTCAAAT CAACTGTCTC TGAAACATTC ATGAGCAAGC CCAGCATTGC TAAGAGGAGA 
GCGAACCAGC AAGAGACGGA ACAGTTTTAT TTCACGAAAA TGAAGGAGTA CTTGGAGGGT 
AGGAACCTCA TCACAAAGCT ACAAGCCAAG CATGACCTCC TGCAGAAAAC CCTGGGAGAA 
AGTCAGCGGA CAGACTGTAG TCTTGCCAGG CGAAGCTCAA CTGTGAGGAA ACAGGATTCC 
AGCCAAGCAA TTCCTCTGGT GGTAGAAAGC TGCATCCGGT TTATTAGCAG ACATGGCCTA 
CAACATGAGG GAATTTTCCG GGTTTCTGGA TCACAAGTAG AAGTGAACGA CATAAAAAAC 
GCCTTTGAGA GAGGAGAGGA TCCCCTGGCT GGGGACCAGA ATGACCATGA CATGGACTCT 
ATAGCTGGTG TCCTCAAGCT TTACTTCCGG GGCCTGGAAC ACCCGCTCTT CCCTAAAGAC 
ATCTTCCATG ACTTGATTGC CTGTGTCACA ATGGACAACC TGCAAGAGAG AGCTGTGCAT 
ATCCGGAAAG TCCTTCTGGT CCTGCCCAAG CCCACTCTGA TTATCATGAG ATATCTCTTT 
GCCTTCCTCA ATCACTTATC ACAGTTCAGT GAAGAGAACA TGATGGACCC CTACAACCTT 
GCCATCTGCT TCGGGCCCTC ACTGATGTCA GTGCCAGAGG GCCACGACCA GGTGTCCTGT 
CAAGCCCACG TGAATGAGCT GATCAAAACC ATCATCATCC AACATGAGAA CATTTTCCCA 
AACCCCAGGG AGCTGGAGGG TCCCATCTAC AGCAGAGGAG GAAGCATGGA GGATTACTGT 
GACAGCACTC ATGGAGAGAC TACCTCTGCT GAAGACTCCA CCCAGGACGT TACAGCGGAG 
CACCACACAA GCGATGATGA ATGTGAGCCC ATAGAAGCCA TTGCCAAGTT TGACTACGTA 
GGCCGGACAG CCCGAGAACT GTCTTTCAAG AAGGGAGCAT CCCTGCTGCT CTACCAGCGA 
GCTTCTGATG ACTGGTGGGA GGGCCGGCAC AACGGTATAG ATGGACTCAT CCCCCATCAG 
TACATCGTAG TCCAAGACAC CGAGGACGGT GTCGTGGAGA GGTCCAGCCC CAAGTCTGAG 
ATTGAGGTCA TGTCTGAGCC ACCTGAAGAA AAGGTGACAG CCAGAACGGG GGCCAGCTGT 
CCCAGTGGGG GTCATGTAGC TGATATTTAT CTTGCAAACA TCAACAAGCA AAGGAAGCGT 
CCAGAATCTG GGAGCATCAG AAAAGCATTT CGGAGTGACA GCCATGGGCT GGGCAGTTCT 
CTGACTGACT CCTCCTCCCT GGGGGTGGGG GCTAGCTGCC GTCCATCCTC CCAGCCCATC 
ATGAGCCAGA ATCTCCCCAA GGAAGGGCCA GATAAGTGTT CCATCAGCGG CCATGGCAGC 
CTCAACTCTA TCAGCCGCCA CTCATCCTTG AAGAACCGGA TGGACAGTCC GCAGATCCGG 
AAGACCGCTA CGGCAGGAAG GTCAAAAAGT TTCAATAACC ATCGGCCCAT GGACCCTGAA 
GTCATTGCAC AGGATATTGA AGCAACAATG AACTCTGCCC TGAATGAGCT TCAAGAGCTA 
GAGCGGCAGA GCAGTGCTAA GCACACACCT GATGTGGTTC TGGACACCTT GGAACCACTC 
AAGACCTCCC CGGTGGTAGC CCCCACATCT GAGCCCTCCA GCCCTCTGCA CACCCAGCTC 
CTCAAGGACC CTGAGCCTGC CTTCCAGCGC AGCGCTAGTA CTGCTGGGGA CATTGCCTGC 
GCCTTCCGGC CTGTAAAGTC TGTCAAGATG GCTGCTCCAG TCAAACCACC AGCCACACGG 
CCCAAGCCAA CTGTCTTCCC CAAAACAAAC GCCACTAGCC CTGGTGTCAA TTCATCTGCT 
TCCCCACAGG CCACTGACAA GTCTTGTACT GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Srgap2 ( XM_006529134.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529197.1 Mus musculus SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu21052
Clone ID Related Accession (Same CDS sequence)	XM_006529134.3 , NM_001081011.2
Accession Version	XM_006529134.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	SLIT-ROBO Rho GTPase-activating protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529134.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCTC CAGCCAAATT CAAAAAGGAT AAGGAGATCA TCGCAGAGTA CGATACTCAG 
GTCAAAGAGA TTAGGGCTCA GCTCACAGAG CAGATGAAAT GCCTAGATCA GCAATGTGAG 
CTCCGGGTGC AGCTGTTGCA GGACCTGCAG GACTTCTTCC GCAAGAAGGC TGAGATTGAA 
ATGGACTACT CTCGAAACCT GGAGAAGCTA GCCGAGCGCT TTCTAGCCAA GACACGAAGC 
ACCAAGGACC AGCAATTTAA GAAGGACCAG AATGTTCTTT CTCCAGTCAA CTGCTGGAAC 
CTCCTCTTAA ACCAAGTGAA GCGGGAGAGC AGGGATCATA CCACCCTGAG TGACATCTAC 
CTGAATAATA TAATTCCTCG ATTTGTTCAA GTCAGCGAGG ACTCAGGAAG ACTCTTTAAA 
AAGAGTAAAG AAGTTGGCCA GCAACTCCAA GATGATTTGA TGAAAGTCCT GAACGAGCTT 
TACTCGGTCA TGAAGACATA TCACATGTAC AATGCCGACA GCATCAGTGC TCAGAGCAAA 
CTGAAGGAAG CAGAGAAACA AGAAGAGAAG CAAATTGGCA AATCAGTAAA GCAAGAGGAC 
CGGCAGACCC CTCGCTCCCC TGACTCCACA GCCAATGTCC GCATTGAGGA GAAGCATGTC 
CGGAGGAGCT CAGTGAAGAA GATTGAGAAG ATGAAGGAGA AGCGACAAGC CAAGTACACA 
GAAAATAAGC TGAAGGCCAT TAAAGCCCGG AATGAGTATT TATTGGCTTT GGAGGCAACC 
AATGCATCTG TCTTCAAGTA CTATATCCAT GACCTGTCTG ATATTATTGA TCAATGTTGT 
GACCTAGGCT ACCATGCTAG CCTGAACCGG GCTCTACGCA CTTTCCTATC TGCTGAATTA 
AATCTGGAAC AGTCAAAACA TGAAGGTCTG GATGCTATTG AAAATGCAGT AGAAAACCTA 
GATGCCACCA GTGACAAGCA ACGGCTCATG GAGATGTACA ACAATGTTTT TTGCCCCCCT 
ATGAAATTTG AATTCCAGCC CCACATGGGA GATATGGCCT CTCAACTCTG TGCCCAGCAG 
CCTGTCCAGA GTGAGCTGGT GCAGAGATGC CAACAGCTGC AGTCTCGCTT ATCCACTTTG 
AAGATTGAGA ATGAAGAGGT GAAAAAGACA ATGGAGGCCA CCCTGCAGAC CATTCAAGAC 
ATTGTGACTG TTGAGGATTT TGATGTATCT GACTGCTTCC AGTATAGCAA TTCCATGGAG 
TCTGTCAAAT CAACTGTCTC TGAAACATTC ATGAGCAAGC CCAGCATTGC TAAGAGGAGA 
GCGAACCAGC AAGAGACGGA ACAGTTTTAT TTCACGAAAA TGAAGGAGTA CTTGGAGGGT 
AGGAACCTCA TCACAAAGCT ACAAGCCAAG CATGACCTCC TGCAGAAAAC CCTGGGAGAA 
AGTCAGCGGA CAGACTGTAG TCTTGCCAGG CGAAGCTCAA CTGTGAGGAA ACAGGATTCC 
AGCCAAGCAA TTCCTCTGGT GGTAGAAAGC TGCATCCGGT TTATTAGCAG ACATGGCCTA 
CAACATGAGG GAATTTTCCG GGTTTCTGGA TCACAAGTAG AAGTGAACGA CATAAAAAAC 
GCCTTTGAGA GAGGAGAGGA TCCCCTGGCT GGGGACCAGA ATGACCATGA CATGGACTCT 
ATAGCTGGTG TCCTCAAGCT TTACTTCCGG GGCCTGGAAC ACCCGCTCTT CCCTAAAGAC 
ATCTTCCATG ACTTGATTGC CTGTGTCACA ATGGACAACC TGCAAGAGAG AGCTGTGCAT 
ATCCGGAAAG TCCTTCTGGT CCTGCCCAAG CCCACTCTGA TTATCATGAG ATATCTCTTT 
GCCTTCCTCA ATCACTTATC ACAGTTCAGT GAAGAGAACA TGATGGACCC CTACAACCTT 
GCCATCTGCT TCGGGCCCTC ACTGATGTCA GTGCCAGAGG GCCACGACCA GGTGTCCTGT 
CAAGCCCACG TGAATGAGCT GATCAAAACC ATCATCATCC AACATGAGAA CATTTTCCCA 
AACCCCAGGG AGCTGGAGGG TCCCATCTAC AGCAGAGGAG GAAGCATGGA GGATTACTGT 
GACAGCACTC ATGGAGAGAC TACCTCTGCT GAAGACTCCA CCCAGGACGT TACAGCGGAG 
CACCACACAA GCGATGATGA ATGTGAGCCC ATAGAAGCCA TTGCCAAGTT TGACTACGTA 
GGCCGGACAG CCCGAGAACT GTCTTTCAAG AAGGGAGCAT CCCTGCTGCT CTACCAGCGA 
GCTTCTGATG ACTGGTGGGA GGGCCGGCAC AACGGTATAG ATGGACTCAT CCCCCATCAG 
TACATCGTAG TCCAAGACAC CGAGGACGGT GTCGTGGAGA GGTCCAGCCC CAAGTCTGAG 
ATTGAGGTCA TGTCTGAGCC ACCTGAAGAA AAGGTGACAG CCAGAACGGG GGCCAGCTGT 
CCCAGTGGGG GTCATGTAGC TGATATTTAT CTTGCAAACA TCAACAAGCA AAGGAAGCGT 
CCAGAATCTG GGAGCATCAG AAAAGCATTT CGGAGTGACA GCCATGGGCT GGGCAGTTCT 
CTGACTGACT CCTCCTCCCT GGGGGTGGGG GCTAGCTGCC GTCCATCCTC CCAGCCCATC 
ATGAGCCAGA ATCTCCCCAA GGAAGGGCCA GATAAGTGTT CCATCAGCGG CCATGGCAGC 
CTCAACTCTA TCAGCCGCCA CTCATCCTTG AAGAACCGGA TGGACAGTCC GCAGATCCGG 
AAGACCGCTA CGGCAGGAAG GTCAAAAAGT TTCAATAACC ATCGGCCCAT GGACCCTGAA 
GTCATTGCAC AGGATATTGA AGCAACAATG AACTCTGCCC TGAATGAGCT TCAAGAGCTA 
GAGCGGCAGA GCAGTGCTAA GCACACACCT GATGTGGTTC TGGACACCTT GGAACCACTC 
AAGACCTCCC CGGTGGTAGC CCCCACATCT GAGCCCTCCA GCCCTCTGCA CACCCAGCTC 
CTCAAGGACC CTGAGCCTGC CTTCCAGCGC AGCGCTAGTA CTGCTGGGGA CATTGCCTGC 
GCCTTCCGGC CTGTAAAGTC TGTCAAGATG GCTGCTCCAG TCAAACCACC AGCCACACGG 
CCCAAGCCAA CTGTCTTCCC CAAAACAAAC GCCACTAGCC CTGGTGTCAA TTCATCTGCT 
TCCCCACAGG CCACTGACAA GTCTTGTACT GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529197.1
CDS	799..4014
Translation	MTSPAKFKKDKEIIAEYDTQVKEIRAQLTEQMKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKLAERFLAKTRSTKDQQFKKDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIYLNNIIPRFVQVSEDSGRLFKKSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISAQSKLKEAEKQEEKQIGKSVKQEDRQTPRSPDSTANVRIEEKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNASVFKYYIHDLSDIIDQCCDLGYHASLNRALRTFLSAELNLEQSKHEGLDAIENAVENLDATSDKQRLMEMYNNVFCPPMKFEFQPHMGDMASQLCAQQPVQSELVQRCQQLQSRLSTLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSMESVKSTVSETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKTLGESQRTDCSLARRSSTVRKQDSSQAIPLVVESCIRFISRHGLQHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLEHPLFPKDIFHDLIACVTMDNLQERAVHIRKVLLVLPKPTLIIMRYLFAFLNHLSQFSEENMMDPYNLAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHENIFPNPRELEGPIYSRGGSMEDYCDSTHGETTSAEDSTQDVTAEHHTSDDECEPIEAIAKFDYVGRTARELSFKKGASLLLYQRASDDWWEGRHNGIDGLIPHQYIVVQDTEDGVVERSSPKSEIEVMSEPPEEKVTARTGASCPSGGHVADIYLANINKQRKRPESGSIRKAFRSDSHGLGSSLTDSSSLGVGASCRPSSQPIMSQNLPKEGPDKCSISGHGSLNSISRHSSLKNRMDSPQIRKTATAGRSKSFNNHRPMDPEVIAQDIEATMNSALNELQELERQSSAKHTPDVVLDTLEPLKTSPVVAPTSEPSSPLHTQLLKDPEPAFQRSASTAGDIACAFRPVKSVKMAAPVKPPATRPKPTVFPKTNATSPGVNSSASPQATDKSCTV

Target ORF information:

RefSeq Version	XM_006529134.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529134.3

ORF Insert Sequence:

ATGACGTCTC CAGCCAAATT CAAAAAGGAT AAGGAGATCA TCGCAGAGTA CGATACTCAG 
GTCAAAGAGA TTAGGGCTCA GCTCACAGAG CAGATGAAAT GCCTAGATCA GCAATGTGAG 
CTCCGGGTGC AGCTGTTGCA GGACCTGCAG GACTTCTTCC GCAAGAAGGC TGAGATTGAA 
ATGGACTACT CTCGAAACCT GGAGAAGCTA GCCGAGCGCT TTCTAGCCAA GACACGAAGC 
ACCAAGGACC AGCAATTTAA GAAGGACCAG AATGTTCTTT CTCCAGTCAA CTGCTGGAAC 
CTCCTCTTAA ACCAAGTGAA GCGGGAGAGC AGGGATCATA CCACCCTGAG TGACATCTAC 
CTGAATAATA TAATTCCTCG ATTTGTTCAA GTCAGCGAGG ACTCAGGAAG ACTCTTTAAA 
AAGAGTAAAG AAGTTGGCCA GCAACTCCAA GATGATTTGA TGAAAGTCCT GAACGAGCTT 
TACTCGGTCA TGAAGACATA TCACATGTAC AATGCCGACA GCATCAGTGC TCAGAGCAAA 
CTGAAGGAAG CAGAGAAACA AGAAGAGAAG CAAATTGGCA AATCAGTAAA GCAAGAGGAC 
CGGCAGACCC CTCGCTCCCC TGACTCCACA GCCAATGTCC GCATTGAGGA GAAGCATGTC 
CGGAGGAGCT CAGTGAAGAA GATTGAGAAG ATGAAGGAGA AGCGACAAGC CAAGTACACA 
GAAAATAAGC TGAAGGCCAT TAAAGCCCGG AATGAGTATT TATTGGCTTT GGAGGCAACC 
AATGCATCTG TCTTCAAGTA CTATATCCAT GACCTGTCTG ATATTATTGA TCAATGTTGT 
GACCTAGGCT ACCATGCTAG CCTGAACCGG GCTCTACGCA CTTTCCTATC TGCTGAATTA 
AATCTGGAAC AGTCAAAACA TGAAGGTCTG GATGCTATTG AAAATGCAGT AGAAAACCTA 
GATGCCACCA GTGACAAGCA ACGGCTCATG GAGATGTACA ACAATGTTTT TTGCCCCCCT 
ATGAAATTTG AATTCCAGCC CCACATGGGA GATATGGCCT CTCAACTCTG TGCCCAGCAG 
CCTGTCCAGA GTGAGCTGGT GCAGAGATGC CAACAGCTGC AGTCTCGCTT ATCCACTTTG 
AAGATTGAGA ATGAAGAGGT GAAAAAGACA ATGGAGGCCA CCCTGCAGAC CATTCAAGAC 
ATTGTGACTG TTGAGGATTT TGATGTATCT GACTGCTTCC AGTATAGCAA TTCCATGGAG 
TCTGTCAAAT CAACTGTCTC TGAAACATTC ATGAGCAAGC CCAGCATTGC TAAGAGGAGA 
GCGAACCAGC AAGAGACGGA ACAGTTTTAT TTCACGAAAA TGAAGGAGTA CTTGGAGGGT 
AGGAACCTCA TCACAAAGCT ACAAGCCAAG CATGACCTCC TGCAGAAAAC CCTGGGAGAA 
AGTCAGCGGA CAGACTGTAG TCTTGCCAGG CGAAGCTCAA CTGTGAGGAA ACAGGATTCC 
AGCCAAGCAA TTCCTCTGGT GGTAGAAAGC TGCATCCGGT TTATTAGCAG ACATGGCCTA 
CAACATGAGG GAATTTTCCG GGTTTCTGGA TCACAAGTAG AAGTGAACGA CATAAAAAAC 
GCCTTTGAGA GAGGAGAGGA TCCCCTGGCT GGGGACCAGA ATGACCATGA CATGGACTCT 
ATAGCTGGTG TCCTCAAGCT TTACTTCCGG GGCCTGGAAC ACCCGCTCTT CCCTAAAGAC 
ATCTTCCATG ACTTGATTGC CTGTGTCACA ATGGACAACC TGCAAGAGAG AGCTGTGCAT 
ATCCGGAAAG TCCTTCTGGT CCTGCCCAAG CCCACTCTGA TTATCATGAG ATATCTCTTT 
GCCTTCCTCA ATCACTTATC ACAGTTCAGT GAAGAGAACA TGATGGACCC CTACAACCTT 
GCCATCTGCT TCGGGCCCTC ACTGATGTCA GTGCCAGAGG GCCACGACCA GGTGTCCTGT 
CAAGCCCACG TGAATGAGCT GATCAAAACC ATCATCATCC AACATGAGAA CATTTTCCCA 
AACCCCAGGG AGCTGGAGGG TCCCATCTAC AGCAGAGGAG GAAGCATGGA GGATTACTGT 
GACAGCACTC ATGGAGAGAC TACCTCTGCT GAAGACTCCA CCCAGGACGT TACAGCGGAG 
CACCACACAA GCGATGATGA ATGTGAGCCC ATAGAAGCCA TTGCCAAGTT TGACTACGTA 
GGCCGGACAG CCCGAGAACT GTCTTTCAAG AAGGGAGCAT CCCTGCTGCT CTACCAGCGA 
GCTTCTGATG ACTGGTGGGA GGGCCGGCAC AACGGTATAG ATGGACTCAT CCCCCATCAG 
TACATCGTAG TCCAAGACAC CGAGGACGGT GTCGTGGAGA GGTCCAGCCC CAAGTCTGAG 
ATTGAGGTCA TGTCTGAGCC ACCTGAAGAA AAGGTGACAG CCAGAACGGG GGCCAGCTGT 
CCCAGTGGGG GTCATGTAGC TGATATTTAT CTTGCAAACA TCAACAAGCA AAGGAAGCGT 
CCAGAATCTG GGAGCATCAG AAAAGCATTT CGGAGTGACA GCCATGGGCT GGGCAGTTCT 
CTGACTGACT CCTCCTCCCT GGGGGTGGGG GCTAGCTGCC GTCCATCCTC CCAGCCCATC 
ATGAGCCAGA ATCTCCCCAA GGAAGGGCCA GATAAGTGTT CCATCAGCGG CCATGGCAGC 
CTCAACTCTA TCAGCCGCCA CTCATCCTTG AAGAACCGGA TGGACAGTCC GCAGATCCGG 
AAGACCGCTA CGGCAGGAAG GTCAAAAAGT TTCAATAACC ATCGGCCCAT GGACCCTGAA 
GTCATTGCAC AGGATATTGA AGCAACAATG AACTCTGCCC TGAATGAGCT TCAAGAGCTA 
GAGCGGCAGA GCAGTGCTAA GCACACACCT GATGTGGTTC TGGACACCTT GGAACCACTC 
AAGACCTCCC CGGTGGTAGC CCCCACATCT GAGCCCTCCA GCCCTCTGCA CACCCAGCTC 
CTCAAGGACC CTGAGCCTGC CTTCCAGCGC AGCGCTAGTA CTGCTGGGGA CATTGCCTGC 
GCCTTCCGGC CTGTAAAGTC TGTCAAGATG GCTGCTCCAG TCAAACCACC AGCCACACGG 
CCCAAGCCAA CTGTCTTCCC CAAAACAAAC GCCACTAGCC CTGGTGTCAA TTCATCTGCT 
TCCCCACAGG CCACTGACAA GTCTTGTACT GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dissection of Glomerular Transcriptional Profile in Patients with Diabetic Nephropathy: SRGAP2a Protects Podocyte Structure and Function.
Diabetes(2017 Dec)
Pan Y,Jiang S,Hou Q,Qiu D,Shi J,Wang L,Chen Z,Zhang M,Duan A,Qin W,Zen K,Liu Z

Slit-Robo GTPase-Activating Protein 2 as a metastasis suppressor in osteosarcoma.
Scientific reports639059(2016 Dec)
Marko TA,Shamsan GA,Edwards EN,Hazelton PE,Rathe SK,Cornax I,Overn PR,Varshney J,Diessner BJ,Moriarity BS,O'Sullivan MG,Odde DJ,Largaespada DA

SRGAP2 and Its Human-Specific Paralog Co-Regulate the Development of Excitatory and Inhibitory Synapses.
Neuron91(2)356-69(2016 Jul)
Fossati M,Pizzarelli R,Schmidt ER,Kupferman JV,Stroebel D,Polleux F,Charrier C

The inverse F-BAR domain protein srGAP2 acts through srGAP3 to modulate neuronal differentiation and neurite outgrowth of mouse neuroblastoma cells.
PloS one8(3)e57865(2013)
Ma Y,Mi YJ,Dai YK,Fu HL,Cui DX,Jin WL

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Srgap2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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