tns1b cDNA ORF clone, Danio rerio(zebrafish)

The following tns1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tns1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa144113 XM_021479027.1
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144114 XM_021479028.1
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144115 XM_021479029.1
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144116 XM_021479030.1
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144117 XM_021479031.1
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144118 XM_021479032.1
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144119 XM_021479033.1
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144120 XM_021479034.1
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144121 XM_021479035.1
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144122 XM_021479036.1
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144123 XM_021479037.1
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa60375 XM_017357846.1
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa60376 XM_017357847.1
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa60377 XM_017357848.1
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa60378 XM_017357849.1
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa60379 XM_009304969.2
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa60380 XM_005167931.3
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa60381 XM_017357850.1
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa42135 XM_009304966.2
Latest version!
Danio rerio tensin 1b (tns1b), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa144113
    Clone ID Related Accession (Same CDS sequence) XM_021479027.1
    Accession Version XM_021479027.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product tensin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
    CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA
    GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT
    TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT
    CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC
    CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG
    AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG
    AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT
    AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC
    AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG
    CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG
    ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG
    GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT
    TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT
    TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC
    AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC
    ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC
    TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG
    ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG
    TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC
    ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT
    AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA
    AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC
    CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC
    ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT
    AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC
    ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT
    GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG
    CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG
    GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT
    GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC
    AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC
    CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT
    CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA
    GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA
    GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG
    TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA
    ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA
    GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG
    TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG
    ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA
    GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC
    CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC
    TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT
    ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC
    ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA
    AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA
    TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA
    GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT
    CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG
    TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA
    GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG
    CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC
    TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT
    GGTGAGGTCC TACCCCAAAC CCCTGCCTTC CCTGTCTCGC CACAAACCCC TTACTTCAAC
    ATGTGTCGAT CTCCTCCAGG ATTGGCTAAA ACACCACTGT CCTTTCTGGG CCTGAAGCCC
    CACAACCCTG CTGAGATCCT GCTCAACCAG ACAGGCTCAG AGAGTGAGGA TGAGGAAGAG
    CCTCGTAGTT ATGTGGAGTC TGTGGCGAAG GCAGCAGCAG TACCTGCATC TCCAACATAT
    GACAGCGACA GCTTAGCTCG TCCTGCCGGA CCGTCCCATG CTTACAATGC TCCACTTTCC
    TCCAGCAGCC CCATTCAGAA CCCAGATCCA GTTGAAAGCA CATTGGCTGA CAGCGGGGTC
    GGGATGAATT CTTTATCCCA GGCTGCTGTC GACATTTTCC ATTTCCAGCC CTCCGAAATC
    CCATACCAAC CACCACCACC CCCCTACCCA ACCTCCATGT TGGGTAGCTA CATCAGCCCT
    TCTCCAGCAG AGGCCATGCA AAATAATACC ACCACCATCC AGCCACTTTC TGGAAGTCCT
    GGGACAAGAC ACCACACCAT CCTTTCTGAC ATGGCGTCCG TTCCCAACTT TCAACACAGA
    TTAGCAAATC AGGATGGTCC CATACTTGGG CGACAGCAGG CTCCAGCTAA TGGTCATGTG
    CCTGGAATTG CTGGTAGTCC AGTGCAAGGA AGACACACTA TGGTGTCCCA GGGTACACAA
    AGCAGTCCCA TACTGAGCAG ACAGTATCCT GTCACCCAGG GGATTCAGAG TAGTCCAATT
    CTCAGCAGGC AGCCAATGGG ACAAGCTGTC CAGAGCAGTC CAGTCCTCAA CCGACAGGCA
    TCCCTGAATC AAGCAAACCA AAGCAGTCCA GTGTTGAGTA GACAGCAGTC CATTACCCTA
    CCCAACCAAG GCAGCCCGGT CCTAAGCAGA CAGTCCTCGC TTAGTCATCC CAGTCAGGGT
    AGTCCTGTAC TAGGACGCCA CCCTTCCCTA ACACAGGGTA GCCCCAGCCT GGATAGGCAT
    CCGATGTACA GCGGTTACAC CACTCCAGAA GAGCGACACG GAGCTCTGTC ACGCCAAAGC
    AGCTCTTCAG GATACCAGCC GCCATCTACA CCGTCTTTCC CAGTTTCTCC AGCAGGATAC
    ATCGAGGGAG CGGGATTCAG ACAGGGGAGT CCTGCTTTCC AACCTCAGTT GCCCGAAAAG
    AGACGCATGT CCAGCAGTGA CCGCACCAAT GGTGGCCTGT CCTATGGGAC GCTCAATGGG
    AAGATGTCCT CACCGGTGTC CAGTGGCGGC AGCACTCCGA GTGTTCATTT CTTCCACACA
    CTCCCCGATT TCTCCAAACT CAACATGTGT GATGGAAGTC CAGAAACCAG AATGAACGTG
    AAGTTTGTCC AGGACACGTC CAAATTCTGG TATAAACCAG ATATCTCGAG AGACCAAGCG
    ATTAACATGC TGAAGGATCA AGATCCTGGG GCCTTTGTGA TTCGGGACAG TCATTCATTC
    AGAGGAGCCT ACGGCTTGGC TATGAAAGTG GCTTCACCAC CTCCAACCAC TCAACCTACA
    AAGAAAGCTG GAGATGTCAC TAATGAGTTG GTGAGGCATT TTCTAATTGA GACGAGTCCG
    AAGGGTGTGA GACTGAAGGG CTGCCCCAAC GAGCCGTACT TTGGCTGCCT GTCTGCGCTG
    GTCTACCAGC ACTCCATCAC TCCACTGGCA CTGCCCTGCA AACTAGTCAT ACCAACCAGA
    GATCCTCTGG AAGAGTCACC TGAGATCGCA ACACCAACCA ACCCGGCGGC AGATATGCTC
    AAGCAGGGCG CAGGGCAGAA GAGTTCAGCT GATGGCCATG CATGCAATGT GCTGTACATC
    AACTCTGTGG ATATGGAGTC TTTGACTGGA CCTCAAGCCA TCGCCAAAGC CATTTCAGAA
    ACCATGAACG CCAGTCCGGC ACCCTCAGCC ACCATTGTTC ACTTCAAGGT GTCGGCACAA
    GGCATCACAC TCACAGACAA CCAGAGAAAG CTTTTCTTCA GACGTCATTA TCCCATCGGC
    ACAGTCACGT TCTGTGACAT TGATCCACAG GAGAGGAAGT GGAACAAACC AGAGGGTGGC
    ACTGCCAAAT TCTTTGGCTT TGTGGCTAGA AAGCAAGGCA GCACGACAGA CAATGTCAGT
    CACCTGTTTG CTGAGATGGA CCCAGATCAG CCGGCCACCG CCATCGTCAA CTTTGTCTCT
    AAAGTGATGA TCAGCTCTCA GAAGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334702.1
    CDS285..5774
    Translation

    Target ORF information:

    RefSeq Version XM_021479027.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479027.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
    CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA
    GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT
    TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT
    CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC
    CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG
    AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG
    AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT
    AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC
    AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG
    CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG
    ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG
    GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT
    TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT
    TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC
    AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC
    ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC
    TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG
    ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG
    TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC
    ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT
    AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA
    AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC
    CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC
    ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT
    AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC
    ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT
    GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG
    CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG
    GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT
    GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC
    AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC
    CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT
    CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA
    GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA
    GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG
    TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA
    ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA
    GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG
    TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG
    ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA
    GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC
    CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC
    TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT
    ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC
    ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA
    AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA
    TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA
    GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT
    CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG
    TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA
    GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG
    CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC
    TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT
    GGTGAGGTCC TACCCCAAAC CCCTGCCTTC CCTGTCTCGC CACAAACCCC TTACTTCAAC
    ATGTGTCGAT CTCCTCCAGG ATTGGCTAAA ACACCACTGT CCTTTCTGGG CCTGAAGCCC
    CACAACCCTG CTGAGATCCT GCTCAACCAG ACAGGCTCAG AGAGTGAGGA TGAGGAAGAG
    CCTCGTAGTT ATGTGGAGTC TGTGGCGAAG GCAGCAGCAG TACCTGCATC TCCAACATAT
    GACAGCGACA GCTTAGCTCG TCCTGCCGGA CCGTCCCATG CTTACAATGC TCCACTTTCC
    TCCAGCAGCC CCATTCAGAA CCCAGATCCA GTTGAAAGCA CATTGGCTGA CAGCGGGGTC
    GGGATGAATT CTTTATCCCA GGCTGCTGTC GACATTTTCC ATTTCCAGCC CTCCGAAATC
    CCATACCAAC CACCACCACC CCCCTACCCA ACCTCCATGT TGGGTAGCTA CATCAGCCCT
    TCTCCAGCAG AGGCCATGCA AAATAATACC ACCACCATCC AGCCACTTTC TGGAAGTCCT
    GGGACAAGAC ACCACACCAT CCTTTCTGAC ATGGCGTCCG TTCCCAACTT TCAACACAGA
    TTAGCAAATC AGGATGGTCC CATACTTGGG CGACAGCAGG CTCCAGCTAA TGGTCATGTG
    CCTGGAATTG CTGGTAGTCC AGTGCAAGGA AGACACACTA TGGTGTCCCA GGGTACACAA
    AGCAGTCCCA TACTGAGCAG ACAGTATCCT GTCACCCAGG GGATTCAGAG TAGTCCAATT
    CTCAGCAGGC AGCCAATGGG ACAAGCTGTC CAGAGCAGTC CAGTCCTCAA CCGACAGGCA
    TCCCTGAATC AAGCAAACCA AAGCAGTCCA GTGTTGAGTA GACAGCAGTC CATTACCCTA
    CCCAACCAAG GCAGCCCGGT CCTAAGCAGA CAGTCCTCGC TTAGTCATCC CAGTCAGGGT
    AGTCCTGTAC TAGGACGCCA CCCTTCCCTA ACACAGGGTA GCCCCAGCCT GGATAGGCAT
    CCGATGTACA GCGGTTACAC CACTCCAGAA GAGCGACACG GAGCTCTGTC ACGCCAAAGC
    AGCTCTTCAG GATACCAGCC GCCATCTACA CCGTCTTTCC CAGTTTCTCC AGCAGGATAC
    ATCGAGGGAG CGGGATTCAG ACAGGGGAGT CCTGCTTTCC AACCTCAGTT GCCCGAAAAG
    AGACGCATGT CCAGCAGTGA CCGCACCAAT GGTGGCCTGT CCTATGGGAC GCTCAATGGG
    AAGATGTCCT CACCGGTGTC CAGTGGCGGC AGCACTCCGA GTGTTCATTT CTTCCACACA
    CTCCCCGATT TCTCCAAACT CAACATGTGT GATGGAAGTC CAGAAACCAG AATGAACGTG
    AAGTTTGTCC AGGACACGTC CAAATTCTGG TATAAACCAG ATATCTCGAG AGACCAAGCG
    ATTAACATGC TGAAGGATCA AGATCCTGGG GCCTTTGTGA TTCGGGACAG TCATTCATTC
    AGAGGAGCCT ACGGCTTGGC TATGAAAGTG GCTTCACCAC CTCCAACCAC TCAACCTACA
    AAGAAAGCTG GAGATGTCAC TAATGAGTTG GTGAGGCATT TTCTAATTGA GACGAGTCCG
    AAGGGTGTGA GACTGAAGGG CTGCCCCAAC GAGCCGTACT TTGGCTGCCT GTCTGCGCTG
    GTCTACCAGC ACTCCATCAC TCCACTGGCA CTGCCCTGCA AACTAGTCAT ACCAACCAGA
    GATCCTCTGG AAGAGTCACC TGAGATCGCA ACACCAACCA ACCCGGCGGC AGATATGCTC
    AAGCAGGGCG CAGGGCAGAA GAGTTCAGCT GATGGCCATG CATGCAATGT GCTGTACATC
    AACTCTGTGG ATATGGAGTC TTTGACTGGA CCTCAAGCCA TCGCCAAAGC CATTTCAGAA
    ACCATGAACG CCAGTCCGGC ACCCTCAGCC ACCATTGTTC ACTTCAAGGT GTCGGCACAA
    GGCATCACAC TCACAGACAA CCAGAGAAAG CTTTTCTTCA GACGTCATTA TCCCATCGGC
    ACAGTCACGT TCTGTGACAT TGATCCACAG GAGAGGAAGT GGAACAAACC AGAGGGTGGC
    ACTGCCAAAT TCTTTGGCTT TGTGGCTAGA AAGCAAGGCA GCACGACAGA CAATGTCAGT
    CACCTGTTTG CTGAGATGGA CCCAGATCAG CCGGCCACCG CCATCGTCAA CTTTGTCTCT
    AAAGTGATGA TCAGCTCTCA GAAGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144114
    Clone ID Related Accession (Same CDS sequence) XM_021479028.1
    Accession Version XM_021479028.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product tensin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
    CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA
    GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT
    TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT
    CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC
    CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG
    AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG
    AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT
    AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC
    AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG
    CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG
    ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG
    GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT
    TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT
    TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC
    AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC
    ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC
    TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG
    ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG
    TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC
    ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT
    AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA
    AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC
    CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC
    ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT
    AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC
    ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT
    GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG
    CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG
    GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT
    GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC
    AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC
    CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT
    CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA
    GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA
    GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG
    TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA
    ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA
    GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG
    TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG
    ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA
    GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC
    CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC
    TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT
    ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC
    ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA
    AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA
    TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA
    GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT
    CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG
    TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA
    GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG
    CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC
    TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT
    GGTGAGGTCC TACCCCAAAC CCCTGCCTTC CCTGTCTCGC CACAAACCCC TTACTTCAAC
    ATGTGTCGAT CTCCTCCAGG ATTGGCTAAA ACACCACTGT CCTTTCTGGG CCTGAAGCCC
    CACAACCCTG CTGAGATCCT GCTCAACCAG ACAGGCTCAG AGCCTCGTAG TTATGTGGAG
    TCTGTGGCGA AGGCAGCAGC AGTACCTGCA TCTCCAACAT ATGACAGCGA CAGCTTAGCT
    CGTCCTGCCG GACCGTCCCA TGCTTACAAT GCTCCACTTT CCTCCAGCAG CCCCATTCAG
    AACCCAGATC CAGTTGAAAG CACATTGGCT GACAGCGGGG TCGGGATGAA TTCTTTATCC
    CAGGCTGCTG TCGACATTTT CCATTTCCAG CCCTCCGAAA TCCCATACCA ACCACCACCA
    CCCCCCTACC CAACCTCCAT GTTGGGTAGC TACATCAGCC CTTCTCCAGC AGAGGCCATG
    CAAAATAATA CCACCACCAT CCAGCCACTT TCTGGAAGTC CTGGGACAAG ACACCACACC
    ATCCTTTCTG ACATGGCGTC CGTTCCCAAC TTTCAACACA GATTAGCAAA TCAGGATGGT
    CCCATACTTG GGCGACAGCA GGCTCCAGCT AATGGTCATG TGCCTGGAAT TGCTGGTAGT
    CCAGTGCAAG GAAGACACAC TATGGTGTCC CAGGGTACAC AAAGCAGTCC CATACTGAGC
    AGACAGTATC CTGTCACCCA GGGGATTCAG AGTAGTCCAA TTCTCAGCAG GCAGCCAATG
    GGACAAGCTG TCCAGAGCAG TCCAGTCCTC AACCGACAGG CATCCCTGAA TCAAGCAAAC
    CAAAGCAGTC CAGTGTTGAG TAGACAGCAG TCCATTACCC TACCCAACCA AGGCAGCCCG
    GTCCTAAGCA GACAGTCCTC GCTTAGTCAT CCCAGTCAGG GTAGTCCTGT ACTAGGACGC
    CACCCTTCCC TAACACAGGG TAGCCCCAGC CTGGATAGGC ATCCGATGTA CAGCGGTTAC
    ACCACTCCAG AAGAGCGACA CGGAGCTCTG TCACGCCAAA GCAGCTCTTC AGGATACCAG
    CCGCCATCTA CACCGTCTTT CCCAGTTTCT CCAGCAGGAT ACATCGAGGG AGCGGGATTC
    AGACAGGGGA GTCCTGCTTT CCAACCTCAG TTGCCCGAAA AGAGACGCAT GTCCAGCAGT
    GACCGCACCA ATGGTGGCCT GTCCTATGGG ACGCTCAATG GGAAGATGTC CTCACCGGTG
    TCCAGTGGCG GCAGCACTCC GAGTGTTCAT TTCTTCCACA CACTCCCCGA TTTCTCCAAA
    CTCAACATGT GTGATGGAAG TCCAGAAACC AGAATGAACG TGAAGTTTGT CCAGGACACG
    TCCAAATTCT GGTATAAACC AGATATCTCG AGAGACCAAG CGATTAACAT GCTGAAGGAT
    CAAGATCCTG GGGCCTTTGT GATTCGGGAC AGTCATTCAT TCAGAGGAGC CTACGGCTTG
    GCTATGAAAG TGGCTTCACC ACCTCCAACC ACTCAACCTA CAAAGAAAGC TGGAGATGTC
    ACTAATGAGT TGGTGAGGCA TTTTCTAATT GAGACGAGTC CGAAGGGTGT GAGACTGAAG
    GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC CTGTCTGCGC TGGTCTACCA GCACTCCATC
    ACTCCACTGG CACTGCCCTG CAAACTAGTC ATACCAACCA GAGATCCTCT GGAAGAGTCA
    CCTGAGATCG CAACACCAAC CAACCCGGCG GCAGATATGC TCAAGCAGGG CGCAGGGCAG
    AAGAGTTCAG CTGATGGCCA TGCATGCAAT GTGCTGTACA TCAACTCTGT GGATATGGAG
    TCTTTGACTG GACCTCAAGC CATCGCCAAA GCCATTTCAG AAACCATGAA CGCCAGTCCG
    GCACCCTCAG CCACCATTGT TCACTTCAAG GTGTCGGCAC AAGGCATCAC ACTCACAGAC
    AACCAGAGAA AGCTTTTCTT CAGACGTCAT TATCCCATCG GCACAGTCAC GTTCTGTGAC
    ATTGATCCAC AGGAGAGGAA GTGGAACAAA CCAGAGGGTG GCACTGCCAA ATTCTTTGGC
    TTTGTGGCTA GAAAGCAAGG CAGCACGACA GACAATGTCA GTCACCTGTT TGCTGAGATG
    GACCCAGATC AGCCGGCCAC CGCCATCGTC AACTTTGTCT CTAAAGTGAT GATCAGCTCT
    CAGAAGCGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334703.1
    CDS284..5755
    Translation

    Target ORF information:

    RefSeq Version XM_021479028.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479028.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
    CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA
    GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT
    TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT
    CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC
    CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG
    AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG
    AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT
    AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC
    AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG
    CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG
    ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG
    GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT
    TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT
    TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC
    AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC
    ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC
    TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG
    ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG
    TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC
    ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT
    AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA
    AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC
    CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC
    ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT
    AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC
    ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT
    GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG
    CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG
    GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT
    GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC
    AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC
    CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT
    CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA
    GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA
    GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG
    TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA
    ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA
    GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG
    TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG
    ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA
    GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC
    CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC
    TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT
    ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC
    ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA
    AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA
    TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA
    GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT
    CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG
    TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA
    GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG
    CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC
    TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT
    GGTGAGGTCC TACCCCAAAC CCCTGCCTTC CCTGTCTCGC CACAAACCCC TTACTTCAAC
    ATGTGTCGAT CTCCTCCAGG ATTGGCTAAA ACACCACTGT CCTTTCTGGG CCTGAAGCCC
    CACAACCCTG CTGAGATCCT GCTCAACCAG ACAGGCTCAG AGCCTCGTAG TTATGTGGAG
    TCTGTGGCGA AGGCAGCAGC AGTACCTGCA TCTCCAACAT ATGACAGCGA CAGCTTAGCT
    CGTCCTGCCG GACCGTCCCA TGCTTACAAT GCTCCACTTT CCTCCAGCAG CCCCATTCAG
    AACCCAGATC CAGTTGAAAG CACATTGGCT GACAGCGGGG TCGGGATGAA TTCTTTATCC
    CAGGCTGCTG TCGACATTTT CCATTTCCAG CCCTCCGAAA TCCCATACCA ACCACCACCA
    CCCCCCTACC CAACCTCCAT GTTGGGTAGC TACATCAGCC CTTCTCCAGC AGAGGCCATG
    CAAAATAATA CCACCACCAT CCAGCCACTT TCTGGAAGTC CTGGGACAAG ACACCACACC
    ATCCTTTCTG ACATGGCGTC CGTTCCCAAC TTTCAACACA GATTAGCAAA TCAGGATGGT
    CCCATACTTG GGCGACAGCA GGCTCCAGCT AATGGTCATG TGCCTGGAAT TGCTGGTAGT
    CCAGTGCAAG GAAGACACAC TATGGTGTCC CAGGGTACAC AAAGCAGTCC CATACTGAGC
    AGACAGTATC CTGTCACCCA GGGGATTCAG AGTAGTCCAA TTCTCAGCAG GCAGCCAATG
    GGACAAGCTG TCCAGAGCAG TCCAGTCCTC AACCGACAGG CATCCCTGAA TCAAGCAAAC
    CAAAGCAGTC CAGTGTTGAG TAGACAGCAG TCCATTACCC TACCCAACCA AGGCAGCCCG
    GTCCTAAGCA GACAGTCCTC GCTTAGTCAT CCCAGTCAGG GTAGTCCTGT ACTAGGACGC
    CACCCTTCCC TAACACAGGG TAGCCCCAGC CTGGATAGGC ATCCGATGTA CAGCGGTTAC
    ACCACTCCAG AAGAGCGACA CGGAGCTCTG TCACGCCAAA GCAGCTCTTC AGGATACCAG
    CCGCCATCTA CACCGTCTTT CCCAGTTTCT CCAGCAGGAT ACATCGAGGG AGCGGGATTC
    AGACAGGGGA GTCCTGCTTT CCAACCTCAG TTGCCCGAAA AGAGACGCAT GTCCAGCAGT
    GACCGCACCA ATGGTGGCCT GTCCTATGGG ACGCTCAATG GGAAGATGTC CTCACCGGTG
    TCCAGTGGCG GCAGCACTCC GAGTGTTCAT TTCTTCCACA CACTCCCCGA TTTCTCCAAA
    CTCAACATGT GTGATGGAAG TCCAGAAACC AGAATGAACG TGAAGTTTGT CCAGGACACG
    TCCAAATTCT GGTATAAACC AGATATCTCG AGAGACCAAG CGATTAACAT GCTGAAGGAT
    CAAGATCCTG GGGCCTTTGT GATTCGGGAC AGTCATTCAT TCAGAGGAGC CTACGGCTTG
    GCTATGAAAG TGGCTTCACC ACCTCCAACC ACTCAACCTA CAAAGAAAGC TGGAGATGTC
    ACTAATGAGT TGGTGAGGCA TTTTCTAATT GAGACGAGTC CGAAGGGTGT GAGACTGAAG
    GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC CTGTCTGCGC TGGTCTACCA GCACTCCATC
    ACTCCACTGG CACTGCCCTG CAAACTAGTC ATACCAACCA GAGATCCTCT GGAAGAGTCA
    CCTGAGATCG CAACACCAAC CAACCCGGCG GCAGATATGC TCAAGCAGGG CGCAGGGCAG
    AAGAGTTCAG CTGATGGCCA TGCATGCAAT GTGCTGTACA TCAACTCTGT GGATATGGAG
    TCTTTGACTG GACCTCAAGC CATCGCCAAA GCCATTTCAG AAACCATGAA CGCCAGTCCG
    GCACCCTCAG CCACCATTGT TCACTTCAAG GTGTCGGCAC AAGGCATCAC ACTCACAGAC
    AACCAGAGAA AGCTTTTCTT CAGACGTCAT TATCCCATCG GCACAGTCAC GTTCTGTGAC
    ATTGATCCAC AGGAGAGGAA GTGGAACAAA CCAGAGGGTG GCACTGCCAA ATTCTTTGGC
    TTTGTGGCTA GAAAGCAAGG CAGCACGACA GACAATGTCA GTCACCTGTT TGCTGAGATG
    GACCCAGATC AGCCGGCCAC CGCCATCGTC AACTTTGTCT CTAAAGTGAT GATCAGCTCT
    CAGAAGCGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144115
    Clone ID Related Accession (Same CDS sequence) XM_021479029.1
    Accession Version XM_021479029.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product tensin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
    TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG
    CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG
    GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG
    TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA
    CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG
    TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC
    ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC
    GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC
    TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT
    GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG
    GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA
    GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC
    GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG
    GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT
    AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC
    GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC
    ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA
    CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC
    CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT
    ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT
    GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT
    GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT
    TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA
    GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG
    GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT
    CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC
    ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT
    TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA
    GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT
    GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA
    GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT
    CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA
    GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG
    CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG
    TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA
    GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG
    AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC
    TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA
    GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT
    ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA
    CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC
    CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT
    TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT
    TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA
    GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG
    CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA
    CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGAGTACACT
    GCTCGTATCC GCTGCATCAA TGACAGCCAG AGTGCTCCCG AGTTTGGCCA TCGCCGCCGC
    TCTCTCTCCT TCAGTGGACT TCCATCTGGT GAAATGTCGC CAGATGAGGC TGCAGTTCCT
    GGACGCTGTC GGACCCTTAG TGAGGGCCTT TCCCAAGACG AAAGCCCAAC TCGAGGAAGC
    TCAGTCCGCT CCCCCATTCG CTGCATCTCA CCAGAGCTGG CCAATACAAT TGCTCTGAAC
    CCTGGGGGGC GGCCCAAGGA GAGGCACATG CACAGCTACA GAGAAGCGTT TGAGGAAATG
    GATGGGGGTC CCGTTAGCCC CCCTCCAATT GTTGGTGGTG AGGTCCTACC CCAAACCCCT
    GCCTTCCCTG TCTCGCCACA AACCCCTTAC TTCAACATGT GTCGATCTCC TCCAGGATTG
    GCTAAAACAC CACTGTCCTT TCTGGGCCTG AAGCCCCACA ACCCTGCTGA GATCCTGCTC
    AACCAGACAG GCTCAGAGAG TGAGGATGAG GAAGAGCCTC GTAGTTATGT GGAGTCTGTG
    GCGAAGGCAG CAGCAGTACC TGCATCTCCA ACATATGACA GCGACAGCTT AGCTCGTCCT
    GCCGGACCGT CCCATGCTTA CAATGCTCCA CTTTCCTCCA GCAGCCCCAT TCAGAACCCA
    GATCCAGTTG AAAGCACATT GGCTGACAGC GGGGTCGGGA TGAATTCTTT ATCCCAGGCT
    GCTGTCGACA TTTTCCATTT CCAGCCCTCC GAAATCCCAT ACCAACCACC ACCACCCCCC
    TACCCAACCT CCATGTTGGG TAGCTACATC AGCCCTTCTC CAGCAGAGGC CATGCAAAAT
    AATACCACCA CCATCCAGCC ACTTTCTGGA AGTCCTGGGA CAAGACACCA CACCATCCTT
    TCTGACATGG CGTCCGTTCC CAACTTTCAA CACAGATTAG CAAATCAGGA TGGTCCCATA
    CTTGGGCGAC AGCAGGCTCC AGCTAATGGT CATGTGCCTG GAATTGCTGG TAGTCCAGTG
    CAAGGAAGAC ACACTATGGT GTCCCAGGGT ACACAAAGCA GTCCCATACT GAGCAGACAG
    TATCCTGTCA CCCAGGGGAT TCAGAGTAGT CCAATTCTCA GCAGGCAGCC AATGGGACAA
    GCTGTCCAGA GCAGTCCAGT CCTCAACCGA CAGGCATCCC TGAATCAAGC AAACCAAAGC
    AGTCCAGTGT TGAGTAGACA GCAGTCCATT ACCCTACCCA ACCAAGGCAG CCCGGTCCTA
    AGCAGACAGT CCTCGCTTAG TCATCCCAGT CAGGGTAGTC CTGTACTAGG ACGCCACCCT
    TCCCTAACAC AGGGTAGCCC CAGCCTGGAT AGGCATCCGA TGTACAGCGG TTACACCACT
    CCAGAAGAGC GACACGGAGC TCTGTCACGC CAAAGCAGCT CTTCAGGATA CCAGCCGCCA
    TCTACACCGT CTTTCCCAGT TTCTCCAGCA GGATACATCG AGGGAGCGGG ATTCAGACAG
    GGGAGTCCTG CTTTCCAACC TCAGTTGCCC GAAAAGAGAC GCATGTCCAG CAGTGACCGC
    ACCAATGGTG GCCTGTCCTA TGGGACGCTC AATGGGAAGA TGTCCTCACC GGTGTCCAGT
    GGCGGCAGCA CTCCGAGTGT TCATTTCTTC CACACACTCC CCGATTTCTC CAAACTCAAC
    ATGTGTGATG GAAGTCCAGA AACCAGAATG AACGTGAAGT TTGTCCAGGA CACGTCCAAA
    TTCTGGTATA AACCAGATAT CTCGAGAGAC CAAGCGATTA ACATGCTGAA GGATCAAGAT
    CCTGGGGCCT TTGTGATTCG GGACAGTCAT TCATTCAGAG GAGCCTACGG CTTGGCTATG
    AAAGTGGCTT CACCACCTCC AACCACTCAA CCTACAAAGA AAGCTGGAGA TGTCACTAAT
    GAGTTGGTGA GGCATTTTCT AATTGAGACG AGTCCGAAGG GTGTGAGACT GAAGGGCTGC
    CCCAACGAGC CGTACTTTGG CTGCCTGTCT GCGCTGGTCT ACCAGCACTC CATCACTCCA
    CTGGCACTGC CCTGCAAACT AGTCATACCA ACCAGAGATC CTCTGGAAGA GTCACCTGAG
    ATCGCAACAC CAACCAACCC GGCGGCAGAT ATGCTCAAGC AGGGCGCAGG GCAGAAGAGT
    TCAGCTGATG GCCATGCATG CAATGTGCTG TACATCAACT CTGTGGATAT GGAGTCTTTG
    ACTGGACCTC AAGCCATCGC CAAAGCCATT TCAGAAACCA TGAACGCCAG TCCGGCACCC
    TCAGCCACCA TTGTTCACTT CAAGGTGTCG GCACAAGGCA TCACACTCAC AGACAACCAG
    AGAAAGCTTT TCTTCAGACG TCATTATCCC ATCGGCACAG TCACGTTCTG TGACATTGAT
    CCACAGGAGA GGAAGTGGAA CAAACCAGAG GGTGGCACTG CCAAATTCTT TGGCTTTGTG
    GCTAGAAAGC AAGGCAGCAC GACAGACAAT GTCAGTCACC TGTTTGCTGA GATGGACCCA
    GATCAGCCGG CCACCGCCAT CGTCAACTTT GTCTCTAAAG TGATGATCAG CTCTCAGAAG
    CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334704.1
    CDS815..6280
    Translation

    Target ORF information:

    RefSeq Version XM_021479029.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479029.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
    TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG
    CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG
    GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG
    TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA
    CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG
    TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC
    ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC
    GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC
    TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT
    GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG
    GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA
    GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC
    GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG
    GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT
    AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC
    GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC
    ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA
    CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC
    CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT
    ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT
    GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT
    GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT
    TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA
    GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG
    GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT
    CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC
    ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT
    TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA
    GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT
    GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA
    GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT
    CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA
    GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG
    CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG
    TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA
    GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG
    AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC
    TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA
    GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT
    ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA
    CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC
    CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT
    TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT
    TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA
    GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG
    CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA
    CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGAGTACACT
    GCTCGTATCC GCTGCATCAA TGACAGCCAG AGTGCTCCCG AGTTTGGCCA TCGCCGCCGC
    TCTCTCTCCT TCAGTGGACT TCCATCTGGT GAAATGTCGC CAGATGAGGC TGCAGTTCCT
    GGACGCTGTC GGACCCTTAG TGAGGGCCTT TCCCAAGACG AAAGCCCAAC TCGAGGAAGC
    TCAGTCCGCT CCCCCATTCG CTGCATCTCA CCAGAGCTGG CCAATACAAT TGCTCTGAAC
    CCTGGGGGGC GGCCCAAGGA GAGGCACATG CACAGCTACA GAGAAGCGTT TGAGGAAATG
    GATGGGGGTC CCGTTAGCCC CCCTCCAATT GTTGGTGGTG AGGTCCTACC CCAAACCCCT
    GCCTTCCCTG TCTCGCCACA AACCCCTTAC TTCAACATGT GTCGATCTCC TCCAGGATTG
    GCTAAAACAC CACTGTCCTT TCTGGGCCTG AAGCCCCACA ACCCTGCTGA GATCCTGCTC
    AACCAGACAG GCTCAGAGAG TGAGGATGAG GAAGAGCCTC GTAGTTATGT GGAGTCTGTG
    GCGAAGGCAG CAGCAGTACC TGCATCTCCA ACATATGACA GCGACAGCTT AGCTCGTCCT
    GCCGGACCGT CCCATGCTTA CAATGCTCCA CTTTCCTCCA GCAGCCCCAT TCAGAACCCA
    GATCCAGTTG AAAGCACATT GGCTGACAGC GGGGTCGGGA TGAATTCTTT ATCCCAGGCT
    GCTGTCGACA TTTTCCATTT CCAGCCCTCC GAAATCCCAT ACCAACCACC ACCACCCCCC
    TACCCAACCT CCATGTTGGG TAGCTACATC AGCCCTTCTC CAGCAGAGGC CATGCAAAAT
    AATACCACCA CCATCCAGCC ACTTTCTGGA AGTCCTGGGA CAAGACACCA CACCATCCTT
    TCTGACATGG CGTCCGTTCC CAACTTTCAA CACAGATTAG CAAATCAGGA TGGTCCCATA
    CTTGGGCGAC AGCAGGCTCC AGCTAATGGT CATGTGCCTG GAATTGCTGG TAGTCCAGTG
    CAAGGAAGAC ACACTATGGT GTCCCAGGGT ACACAAAGCA GTCCCATACT GAGCAGACAG
    TATCCTGTCA CCCAGGGGAT TCAGAGTAGT CCAATTCTCA GCAGGCAGCC AATGGGACAA
    GCTGTCCAGA GCAGTCCAGT CCTCAACCGA CAGGCATCCC TGAATCAAGC AAACCAAAGC
    AGTCCAGTGT TGAGTAGACA GCAGTCCATT ACCCTACCCA ACCAAGGCAG CCCGGTCCTA
    AGCAGACAGT CCTCGCTTAG TCATCCCAGT CAGGGTAGTC CTGTACTAGG ACGCCACCCT
    TCCCTAACAC AGGGTAGCCC CAGCCTGGAT AGGCATCCGA TGTACAGCGG TTACACCACT
    CCAGAAGAGC GACACGGAGC TCTGTCACGC CAAAGCAGCT CTTCAGGATA CCAGCCGCCA
    TCTACACCGT CTTTCCCAGT TTCTCCAGCA GGATACATCG AGGGAGCGGG ATTCAGACAG
    GGGAGTCCTG CTTTCCAACC TCAGTTGCCC GAAAAGAGAC GCATGTCCAG CAGTGACCGC
    ACCAATGGTG GCCTGTCCTA TGGGACGCTC AATGGGAAGA TGTCCTCACC GGTGTCCAGT
    GGCGGCAGCA CTCCGAGTGT TCATTTCTTC CACACACTCC CCGATTTCTC CAAACTCAAC
    ATGTGTGATG GAAGTCCAGA AACCAGAATG AACGTGAAGT TTGTCCAGGA CACGTCCAAA
    TTCTGGTATA AACCAGATAT CTCGAGAGAC CAAGCGATTA ACATGCTGAA GGATCAAGAT
    CCTGGGGCCT TTGTGATTCG GGACAGTCAT TCATTCAGAG GAGCCTACGG CTTGGCTATG
    AAAGTGGCTT CACCACCTCC AACCACTCAA CCTACAAAGA AAGCTGGAGA TGTCACTAAT
    GAGTTGGTGA GGCATTTTCT AATTGAGACG AGTCCGAAGG GTGTGAGACT GAAGGGCTGC
    CCCAACGAGC CGTACTTTGG CTGCCTGTCT GCGCTGGTCT ACCAGCACTC CATCACTCCA
    CTGGCACTGC CCTGCAAACT AGTCATACCA ACCAGAGATC CTCTGGAAGA GTCACCTGAG
    ATCGCAACAC CAACCAACCC GGCGGCAGAT ATGCTCAAGC AGGGCGCAGG GCAGAAGAGT
    TCAGCTGATG GCCATGCATG CAATGTGCTG TACATCAACT CTGTGGATAT GGAGTCTTTG
    ACTGGACCTC AAGCCATCGC CAAAGCCATT TCAGAAACCA TGAACGCCAG TCCGGCACCC
    TCAGCCACCA TTGTTCACTT CAAGGTGTCG GCACAAGGCA TCACACTCAC AGACAACCAG
    AGAAAGCTTT TCTTCAGACG TCATTATCCC ATCGGCACAG TCACGTTCTG TGACATTGAT
    CCACAGGAGA GGAAGTGGAA CAAACCAGAG GGTGGCACTG CCAAATTCTT TGGCTTTGTG
    GCTAGAAAGC AAGGCAGCAC GACAGACAAT GTCAGTCACC TGTTTGCTGA GATGGACCCA
    GATCAGCCGG CCACCGCCAT CGTCAACTTT GTCTCTAAAG TGATGATCAG CTCTCAGAAG
    CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144116
    Clone ID Related Accession (Same CDS sequence) XM_021479030.1
    Accession Version XM_021479030.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product tensin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCTTGAGT CCAGTTCTTC TTTGGGATTA AATATTCGGG GGCTAACAAG ACGAGTCGGT 
    GAGATGATGT CCGGCTGGAC GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG
    CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG
    GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG
    TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA
    CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG
    TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC
    ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC
    GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC
    TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT
    GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG
    GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA
    GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC
    GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG
    GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT
    AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC
    GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC
    ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA
    CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC
    CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT
    ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT
    GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT
    GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT
    TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA
    GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG
    GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT
    CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC
    ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT
    TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA
    GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT
    GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA
    GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT
    CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA
    GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG
    CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG
    TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA
    GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG
    AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC
    TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA
    GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT
    ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA
    CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC
    CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT
    TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT
    TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA
    GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG
    CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA
    CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGAGTACACT
    GCTCGTATCC GCTGCATCAA TGACAGCCAG AGTGCTCCCG AGTTTGGCCA TCGCCGCCGC
    TCTCTCTCCT TCAGTGGACT TCCATCTGGT GAAATGTCGC CAGATGAGGC TGCAGTTCCT
    GGACGCTGTC GGACCCTTAG TGAGGGCCTT TCCCAAGACG AAAGCCCAAC TCGAGGAAGC
    TCAGTCCGCT CCCCCATTCG CTGCATCTCA CCAGAGCTGG CCAATACAAT TGCTCTGAAC
    CCTGGGGGGC GGCCCAAGGA GAGGCACATG CACAGCTACA GAGAAGCGTT TGAGGAAATG
    GATGGGGGTC CCGTTAGCCC CCCTCCAATT GTTGGTGGTG AGGTCCTACC CCAAACCCCT
    GCCTTCCCTG TCTCGCCACA AACCCCTTAC TTCAACATGT GTCGATCTCC TCCAGGATTG
    GCTAAAACAC CACTGTCCTT TCTGGGCCTG AAGCCCCACA ACCCTGCTGA GATCCTGCTC
    AACCAGACAG GCTCAGAGAG TGAGGATGAG GAAGAGCCTC GTAGTTATGT GGAGTCTGTG
    GCGAAGGCAG CAGCAGTACC TGCATCTCCA ACATATGACA GCGACAGCTT AGCTCGTCCT
    GCCGGACCGT CCCATGCTTA CAATGCTCCA CTTTCCTCCA GCAGCCCCAT TCAGAACCCA
    GATCCAGTTG AAAGCACATT GGCTGACAGC GGGGTCGGGA TGAATTCTTT ATCCCAGGCT
    GCTGTCGACA TTTTCCATTT CCAGCCCTCC GAAATCCCAT ACCAACCACC ACCACCCCCC
    TACCCAACCT CCATGTTGGG TAGCTACATC AGCCCTTCTC CAGCAGAGGC CATGCAAAAT
    AATACCACCA CCATCCAGCC ACTTTCTGGA AGTCCTGGGA CAAGACACCA CACCATCCTT
    TCTGACATGG CGTCCGTTCC CAACTTTCAA CACAGATTAG CAAATCAGGA TGGTCCCATA
    CTTGGGCGAC AGCAGGCTCC AGCTAATGGT CATGTGCCTG GAATTGCTGG TAGTCCAGTG
    CAAGGAAGAC ACACTATGGT GTCCCAGGGT ACACAAAGCA GTCCCATACT GAGCAGACAG
    TATCCTGTCA CCCAGGGGAT TCAGAGTAGT CCAATTCTCA GCAGGCAGCC AATGGGACAA
    GCTGTCCAGA GCAGTCCAGT CCTCAACCGA CAGGCATCCC TGAATCAAGC AAACCAAAGC
    AGTCCAGTGT TGAGTAGACA GCAGTCCATT ACCCTACCCA ACCAAGGCAG CCCGGTCCTA
    AGCAGACAGT CCTCGCTTAG TCATCCCAGT CAGGGTAGTC CTGTACTAGG ACGCCACCCT
    TCCCTAACAC AGGGTAGCCC CAGCCTGGAT AGGCATCCGA TGTACAGCGG TTACACCACT
    CCAGAAGAGC GACACGGAGC TCTGTCACGC CAAAGCAGCT CTTCAGGATA CCAGCCGCCA
    TCTACACCGT CTTTCCCAGT TTCTCCAGCA GGATACATCG AGGGAGCGGG ATTCAGACAG
    GGGAGTCCTG CTTTCCAACC TCAGTTGCCC GAAAAGAGAC GCATGTCCAG CAGTGACCGC
    ACCAATGGTG GCCTGTCCTA TGGGACGCTC AATGGGAAGA TGTCCTCACC GGTGTCCAGT
    GGCGGCAGCA CTCCGAGTGT TCATTTCTTC CACACACTCC CCGATTTCTC CAAACTCAAC
    ATGTGTGATG GAAGTCCAGA AACCAGAATG AACGTGAAGT TTGTCCAGGA CACGTCCAAA
    TTCTGGTATA AACCAGATAT CTCGAGAGAC CAAGCGATTA ACATGCTGAA GGATCAAGAT
    CCTGGGGCCT TTGTGATTCG GGACAGTCAT TCATTCAGAG GAGCCTACGG CTTGGCTATG
    AAAGTGGCTT CACCACCTCC AACCACTCAA CCTACAAAGA AAGCTGGAGA TGTCACTAAT
    GAGTTGGTGA GGCATTTTCT AATTGAGACG AGTCCGAAGG GTGTGAGACT GAAGGGCTGC
    CCCAACGAGC CGTACTTTGG CTGCCTGTCT GCGCTGGTCT ACCAGCACTC CATCACTCCA
    CTGGCACTGC CCTGCAAACT AGTCATACCA ACCAGAGATC CTCTGGAAGA GTCACCTGAG
    ATCGCAACAC CAACCAACCC GGCGGCAGAT ATGCTCAAGC AGGGCGCAGG GCAGAAGAGT
    TCAGCTGATG GCCATGCATG CAATGTGCTG TACATCAACT CTGTGGATAT GGAGTCTTTG
    ACTGGACCTC AAGCCATCGC CAAAGCCATT TCAGAAACCA TGAACGCCAG TCCGGCACCC
    TCAGCCACCA TTGTTCACTT CAAGGTGTCG GCACAAGGCA TCACACTCAC AGACAACCAG
    AGAAAGCTTT TCTTCAGACG TCATTATCCC ATCGGCACAG TCACGTTCTG TGACATTGAT
    CCACAGGAGA GGAAGTGGAA CAAACCAGAG GGTGGCACTG CCAAATTCTT TGGCTTTGTG
    GCTAGAAAGC AAGGCAGCAC GACAGACAAT GTCAGTCACC TGTTTGCTGA GATGGACCCA
    GATCAGCCGG CCACCGCCAT CGTCAACTTT GTCTCTAAAG TGATGATCAG CTCTCAGAAG
    CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334705.1
    CDS222..5687
    Translation

    Target ORF information:

    RefSeq Version XM_021479030.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479030.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCTTGAGT CCAGTTCTTC TTTGGGATTA AATATTCGGG GGCTAACAAG ACGAGTCGGT 
    GAGATGATGT CCGGCTGGAC GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG
    CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG
    GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG
    TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA
    CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG
    TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC
    ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC
    GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC
    TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT
    GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG
    GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA
    GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC
    GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG
    GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT
    AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC
    GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC
    ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA
    CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC
    CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT
    ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT
    GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT
    GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT
    TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA
    GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG
    GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT
    CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC
    ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT
    TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA
    GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT
    GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA
    GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT
    CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA
    GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG
    CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG
    TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA
    GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG
    AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC
    TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA
    GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT
    ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA
    CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC
    CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT
    TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT
    TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA
    GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG
    CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA
    CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGAGTACACT
    GCTCGTATCC GCTGCATCAA TGACAGCCAG AGTGCTCCCG AGTTTGGCCA TCGCCGCCGC
    TCTCTCTCCT TCAGTGGACT TCCATCTGGT GAAATGTCGC CAGATGAGGC TGCAGTTCCT
    GGACGCTGTC GGACCCTTAG TGAGGGCCTT TCCCAAGACG AAAGCCCAAC TCGAGGAAGC
    TCAGTCCGCT CCCCCATTCG CTGCATCTCA CCAGAGCTGG CCAATACAAT TGCTCTGAAC
    CCTGGGGGGC GGCCCAAGGA GAGGCACATG CACAGCTACA GAGAAGCGTT TGAGGAAATG
    GATGGGGGTC CCGTTAGCCC CCCTCCAATT GTTGGTGGTG AGGTCCTACC CCAAACCCCT
    GCCTTCCCTG TCTCGCCACA AACCCCTTAC TTCAACATGT GTCGATCTCC TCCAGGATTG
    GCTAAAACAC CACTGTCCTT TCTGGGCCTG AAGCCCCACA ACCCTGCTGA GATCCTGCTC
    AACCAGACAG GCTCAGAGAG TGAGGATGAG GAAGAGCCTC GTAGTTATGT GGAGTCTGTG
    GCGAAGGCAG CAGCAGTACC TGCATCTCCA ACATATGACA GCGACAGCTT AGCTCGTCCT
    GCCGGACCGT CCCATGCTTA CAATGCTCCA CTTTCCTCCA GCAGCCCCAT TCAGAACCCA
    GATCCAGTTG AAAGCACATT GGCTGACAGC GGGGTCGGGA TGAATTCTTT ATCCCAGGCT
    GCTGTCGACA TTTTCCATTT CCAGCCCTCC GAAATCCCAT ACCAACCACC ACCACCCCCC
    TACCCAACCT CCATGTTGGG TAGCTACATC AGCCCTTCTC CAGCAGAGGC CATGCAAAAT
    AATACCACCA CCATCCAGCC ACTTTCTGGA AGTCCTGGGA CAAGACACCA CACCATCCTT
    TCTGACATGG CGTCCGTTCC CAACTTTCAA CACAGATTAG CAAATCAGGA TGGTCCCATA
    CTTGGGCGAC AGCAGGCTCC AGCTAATGGT CATGTGCCTG GAATTGCTGG TAGTCCAGTG
    CAAGGAAGAC ACACTATGGT GTCCCAGGGT ACACAAAGCA GTCCCATACT GAGCAGACAG
    TATCCTGTCA CCCAGGGGAT TCAGAGTAGT CCAATTCTCA GCAGGCAGCC AATGGGACAA
    GCTGTCCAGA GCAGTCCAGT CCTCAACCGA CAGGCATCCC TGAATCAAGC AAACCAAAGC
    AGTCCAGTGT TGAGTAGACA GCAGTCCATT ACCCTACCCA ACCAAGGCAG CCCGGTCCTA
    AGCAGACAGT CCTCGCTTAG TCATCCCAGT CAGGGTAGTC CTGTACTAGG ACGCCACCCT
    TCCCTAACAC AGGGTAGCCC CAGCCTGGAT AGGCATCCGA TGTACAGCGG TTACACCACT
    CCAGAAGAGC GACACGGAGC TCTGTCACGC CAAAGCAGCT CTTCAGGATA CCAGCCGCCA
    TCTACACCGT CTTTCCCAGT TTCTCCAGCA GGATACATCG AGGGAGCGGG ATTCAGACAG
    GGGAGTCCTG CTTTCCAACC TCAGTTGCCC GAAAAGAGAC GCATGTCCAG CAGTGACCGC
    ACCAATGGTG GCCTGTCCTA TGGGACGCTC AATGGGAAGA TGTCCTCACC GGTGTCCAGT
    GGCGGCAGCA CTCCGAGTGT TCATTTCTTC CACACACTCC CCGATTTCTC CAAACTCAAC
    ATGTGTGATG GAAGTCCAGA AACCAGAATG AACGTGAAGT TTGTCCAGGA CACGTCCAAA
    TTCTGGTATA AACCAGATAT CTCGAGAGAC CAAGCGATTA ACATGCTGAA GGATCAAGAT
    CCTGGGGCCT TTGTGATTCG GGACAGTCAT TCATTCAGAG GAGCCTACGG CTTGGCTATG
    AAAGTGGCTT CACCACCTCC AACCACTCAA CCTACAAAGA AAGCTGGAGA TGTCACTAAT
    GAGTTGGTGA GGCATTTTCT AATTGAGACG AGTCCGAAGG GTGTGAGACT GAAGGGCTGC
    CCCAACGAGC CGTACTTTGG CTGCCTGTCT GCGCTGGTCT ACCAGCACTC CATCACTCCA
    CTGGCACTGC CCTGCAAACT AGTCATACCA ACCAGAGATC CTCTGGAAGA GTCACCTGAG
    ATCGCAACAC CAACCAACCC GGCGGCAGAT ATGCTCAAGC AGGGCGCAGG GCAGAAGAGT
    TCAGCTGATG GCCATGCATG CAATGTGCTG TACATCAACT CTGTGGATAT GGAGTCTTTG
    ACTGGACCTC AAGCCATCGC CAAAGCCATT TCAGAAACCA TGAACGCCAG TCCGGCACCC
    TCAGCCACCA TTGTTCACTT CAAGGTGTCG GCACAAGGCA TCACACTCAC AGACAACCAG
    AGAAAGCTTT TCTTCAGACG TCATTATCCC ATCGGCACAG TCACGTTCTG TGACATTGAT
    CCACAGGAGA GGAAGTGGAA CAAACCAGAG GGTGGCACTG CCAAATTCTT TGGCTTTGTG
    GCTAGAAAGC AAGGCAGCAC GACAGACAAT GTCAGTCACC TGTTTGCTGA GATGGACCCA
    GATCAGCCGG CCACCGCCAT CGTCAACTTT GTCTCTAAAG TGATGATCAG CTCTCAGAAG
    CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144117
    Clone ID Related Accession (Same CDS sequence) XM_021479031.1
    Accession Version XM_021479031.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product tensin-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
    CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA
    GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT
    TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT
    CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC
    CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG
    AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG
    AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT
    AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC
    AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG
    CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG
    ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG
    GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT
    TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT
    TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC
    AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC
    ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC
    TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG
    ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG
    TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC
    ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT
    AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA
    AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC
    CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC
    ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT
    AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC
    ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT
    GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG
    CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG
    GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT
    GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC
    AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC
    CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT
    CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA
    GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA
    GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG
    TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA
    ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA
    GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG
    TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG
    ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA
    GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC
    CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC
    TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT
    ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC
    ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA
    AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA
    TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA
    GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT
    CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG
    TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA
    GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG
    CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC
    TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT
    GGTGAGGTCC TACCCCAAAC CCCTGCCTTC CCTGTCTCGC CACAAACCCC TTACTTCAAC
    ATGTGTCGAT CTCCTCCAGG ATTGGCTAAA ACACCACTGT CCTTTCTGGG CCTGAAGCCC
    CACAACCCTG CTGAGATCCT GCTCAACCAG ACAGGCTCAG AGAGTGAGGA TGAGGAAGAG
    CCTCGTAGTT ATGTGGAGTC TGTGGCGAAG GCAGCAGCAG TACCTGCATC TCCAACATAT
    GACAGCGACA GCTTAGCTCG TCCTGCCGGA CCGTCCCATG CTTACAATGC TCCACTTTCC
    TCCAGCAGCC CCATTCAGAA CCCAGATCCA GTTGAAAGCA CATTGGCTGA CAGCGGGGTC
    GGGATGAATT CTTTATCCCA GGCTGCTGTC GACATTTTCC ATTTCCAGCC CTCCGAAATC
    CCATACCAAC CACCACCACC CCCCTACCCA ACCTCCATGT TGGGTAGCTA CATCAGCCCT
    TCTCCAGCAG AGGCCATGCA AAATAATACC ACCACCATCC AGCCACTTTC TGGAAGTCCT
    GGGACAAGAC ACCACACCAT CCTTTCTGAC ATGGCGTCCG TTCCCAACTT TCAACACAGA
    TTAGCAAATC AGGATGGTCC CATACTTGGG CGACAGCAGG CTCCAGCTAA TGGTCATGTG
    CCTGGAATTG CTGGTAGTCC AGTGCAAGGA AGACACACTA TGGTGTCCCA GGGTACACAA
    AGCAGTCCCA TACTGAGCAG ACAGTATCCT GTCACCCAGG GGATTCAGAG TAGTCCAATT
    CTCAGCAGGC AGCCAATGGG ACAAGCTGTC CAGAGCAGTC CAGTCCTCAA CCGACAGGCA
    TCCCTGAATC AAGCAAACCA AAGCAGTCCA GTGTTGAGTA GACAGCAGTC CATTACCCTA
    CCCAACCAAG GCAGCCCGGT CCTAAGCAGA CAGTCCTCGC TTAGTCATCC CAGTCAGGGT
    AGTCCTGTAC TAGGACGCCA CCCTTCCCTA ACACAGGGTA GCCCCAGCCT GGATAGGCAT
    CCGATGTACA GCGGTTACAC CACTCCAGAA GAGCGACACG GAGCTCTGTC ACGCCAAAGC
    AGCTCTTCAG GATACCAGCC GCCATCTACA CCGTCTTTCC CAGTTTCTCC AGCAGGATAC
    ATCGAGGGAG CGGGATTCAG ACAGGGGAGT CCTGCTTTCC AACCTCAGTT GCCCGAAAAG
    AGACGCATGT CCAGCAGTGA CCGCACCAAT GGTGGCCTGT CCTATGGGAC GCTCAATGGG
    AAGATGTCCT CACCGGTGTC CAGTGGCGGC AGCACTCCGA GTGTTCATTT CTTCCACACA
    CTCCCCGATT TCTCCAAACT CAACATGTGT GATGGAAGTC CAGAAACCAG AATGAACGTG
    AAGTTTGTCC AGGACACGTC CAAATTCTGG TATAAACCAG ATATCTCGAG AGACCAAGCG
    ATTAACATGC TGAAGGATCA AGATCCTGGG GCCTTTGTGA TTCGGGACAG TCATTCATTC
    AGAGGAGCCT ACGGCTTGGC TATGAAAGTG GCTTCACCAC CTCCAACCAC TCAACCTACA
    AAGAAAGCTG GAGATGTCAC TAATGAGTTG GTGAGGCATT TTCTAATTGA GACGAGTCCG
    AAGGGTGTGA GACTGAAGGG CTGCCCCAAC GAGCCGTACT TTGGCTGCCT GTCTGCGCTG
    GTCTACCAGC ACTCCATCAC TCCACTGGCA CTGCCCTGCA AACTAGTCAT ACCAACCAGA
    GATCCTCTGG AAGAGTCACC TGAGATCGCA ACACCAACCA ACCCGGCGGC AGATATGCTC
    AAGCAGGGCG CAGCATGCAA TGTGCTGTAC ATCAACTCTG TGGATATGGA GTCTTTGACT
    GGACCTCAAG CCATCGCCAA AGCCATTTCA GAAACCATGA ACGCCAGTCC GGCACCCTCA
    GCCACCATTG TTCACTTCAA GGTGTCGGCA CAAGGCATCA CACTCACAGA CAACCAGAGA
    AAGCTTTTCT TCAGACGTCA TTATCCCATC GGCACAGTCA CGTTCTGTGA CATTGATCCA
    CAGGAGAGGA AGTGGAACAA ACCAGAGGGT GGCACTGCCA AATTCTTTGG CTTTGTGGCT
    AGAAAGCAAG GCAGCACGAC AGACAATGTC AGTCACCTGT TTGCTGAGAT GGACCCAGAT
    CAGCCGGCCA CCGCCATCGT CAACTTTGTC TCTAAAGTGA TGATCAGCTC TCAGAAGCGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334706.1
    CDS284..5746
    Translation

    Target ORF information:

    RefSeq Version XM_021479031.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479031.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
    CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA
    GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT
    TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT
    CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC
    CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG
    AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG
    AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT
    AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC
    AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG
    CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG
    ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG
    GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT
    TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT
    TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC
    AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC
    ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC
    TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG
    ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG
    TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC
    ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT
    AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA
    AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC
    CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC
    ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT
    AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC
    ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT
    GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG
    CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG
    GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT
    GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC
    AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC
    CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT
    CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA
    GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA
    GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG
    TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA
    ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA
    GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG
    TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG
    ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA
    GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC
    CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC
    TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT
    ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC
    ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA
    AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA
    TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA
    GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT
    CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG
    TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA
    GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG
    CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC
    TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT
    GGTGAGGTCC TACCCCAAAC CCCTGCCTTC CCTGTCTCGC CACAAACCCC TTACTTCAAC
    ATGTGTCGAT CTCCTCCAGG ATTGGCTAAA ACACCACTGT CCTTTCTGGG CCTGAAGCCC
    CACAACCCTG CTGAGATCCT GCTCAACCAG ACAGGCTCAG AGAGTGAGGA TGAGGAAGAG
    CCTCGTAGTT ATGTGGAGTC TGTGGCGAAG GCAGCAGCAG TACCTGCATC TCCAACATAT
    GACAGCGACA GCTTAGCTCG TCCTGCCGGA CCGTCCCATG CTTACAATGC TCCACTTTCC
    TCCAGCAGCC CCATTCAGAA CCCAGATCCA GTTGAAAGCA CATTGGCTGA CAGCGGGGTC
    GGGATGAATT CTTTATCCCA GGCTGCTGTC GACATTTTCC ATTTCCAGCC CTCCGAAATC
    CCATACCAAC CACCACCACC CCCCTACCCA ACCTCCATGT TGGGTAGCTA CATCAGCCCT
    TCTCCAGCAG AGGCCATGCA AAATAATACC ACCACCATCC AGCCACTTTC TGGAAGTCCT
    GGGACAAGAC ACCACACCAT CCTTTCTGAC ATGGCGTCCG TTCCCAACTT TCAACACAGA
    TTAGCAAATC AGGATGGTCC CATACTTGGG CGACAGCAGG CTCCAGCTAA TGGTCATGTG
    CCTGGAATTG CTGGTAGTCC AGTGCAAGGA AGACACACTA TGGTGTCCCA GGGTACACAA
    AGCAGTCCCA TACTGAGCAG ACAGTATCCT GTCACCCAGG GGATTCAGAG TAGTCCAATT
    CTCAGCAGGC AGCCAATGGG ACAAGCTGTC CAGAGCAGTC CAGTCCTCAA CCGACAGGCA
    TCCCTGAATC AAGCAAACCA AAGCAGTCCA GTGTTGAGTA GACAGCAGTC CATTACCCTA
    CCCAACCAAG GCAGCCCGGT CCTAAGCAGA CAGTCCTCGC TTAGTCATCC CAGTCAGGGT
    AGTCCTGTAC TAGGACGCCA CCCTTCCCTA ACACAGGGTA GCCCCAGCCT GGATAGGCAT
    CCGATGTACA GCGGTTACAC CACTCCAGAA GAGCGACACG GAGCTCTGTC ACGCCAAAGC
    AGCTCTTCAG GATACCAGCC GCCATCTACA CCGTCTTTCC CAGTTTCTCC AGCAGGATAC
    ATCGAGGGAG CGGGATTCAG ACAGGGGAGT CCTGCTTTCC AACCTCAGTT GCCCGAAAAG
    AGACGCATGT CCAGCAGTGA CCGCACCAAT GGTGGCCTGT CCTATGGGAC GCTCAATGGG
    AAGATGTCCT CACCGGTGTC CAGTGGCGGC AGCACTCCGA GTGTTCATTT CTTCCACACA
    CTCCCCGATT TCTCCAAACT CAACATGTGT GATGGAAGTC CAGAAACCAG AATGAACGTG
    AAGTTTGTCC AGGACACGTC CAAATTCTGG TATAAACCAG ATATCTCGAG AGACCAAGCG
    ATTAACATGC TGAAGGATCA AGATCCTGGG GCCTTTGTGA TTCGGGACAG TCATTCATTC
    AGAGGAGCCT ACGGCTTGGC TATGAAAGTG GCTTCACCAC CTCCAACCAC TCAACCTACA
    AAGAAAGCTG GAGATGTCAC TAATGAGTTG GTGAGGCATT TTCTAATTGA GACGAGTCCG
    AAGGGTGTGA GACTGAAGGG CTGCCCCAAC GAGCCGTACT TTGGCTGCCT GTCTGCGCTG
    GTCTACCAGC ACTCCATCAC TCCACTGGCA CTGCCCTGCA AACTAGTCAT ACCAACCAGA
    GATCCTCTGG AAGAGTCACC TGAGATCGCA ACACCAACCA ACCCGGCGGC AGATATGCTC
    AAGCAGGGCG CAGCATGCAA TGTGCTGTAC ATCAACTCTG TGGATATGGA GTCTTTGACT
    GGACCTCAAG CCATCGCCAA AGCCATTTCA GAAACCATGA ACGCCAGTCC GGCACCCTCA
    GCCACCATTG TTCACTTCAA GGTGTCGGCA CAAGGCATCA CACTCACAGA CAACCAGAGA
    AAGCTTTTCT TCAGACGTCA TTATCCCATC GGCACAGTCA CGTTCTGTGA CATTGATCCA
    CAGGAGAGGA AGTGGAACAA ACCAGAGGGT GGCACTGCCA AATTCTTTGG CTTTGTGGCT
    AGAAAGCAAG GCAGCACGAC AGACAATGTC AGTCACCTGT TTGCTGAGAT GGACCCAGAT
    CAGCCGGCCA CCGCCATCGT CAACTTTGTC TCTAAAGTGA TGATCAGCTC TCAGAAGCGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144118
    Clone ID Related Accession (Same CDS sequence) XM_021479032.1
    Accession Version XM_021479032.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product tensin-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
    CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA
    GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT
    TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT
    CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC
    CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG
    AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG
    AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT
    AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC
    AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG
    CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG
    ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG
    GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT
    TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT
    TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC
    AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC
    ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC
    TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG
    ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG
    TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC
    ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT
    AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA
    AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC
    CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC
    ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT
    AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC
    ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT
    GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG
    CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG
    GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT
    GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC
    AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC
    CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT
    CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA
    GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA
    GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG
    TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA
    ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA
    GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG
    TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG
    ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA
    GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC
    CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC
    TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT
    ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC
    ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA
    AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA
    TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA
    GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT
    CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG
    TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA
    GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG
    CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC
    TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT
    GGTCGATCTC CTCCAGGATT GGCTAAAACA CCACTGTCCT TTCTGGGCCT GAAGCCCCAC
    AACCCTGCTG AGATCCTGCT CAACCAGACA GGCTCAGAGA GTGAGGATGA GGAAGAGCCT
    CGTAGTTATG TGGAGTCTGT GGCGAAGGCA GCAGCAGTAC CTGCATCTCC AACATATGAC
    AGCGACAGCT TAGCTCGTCC TGCCGGACCG TCCCATGCTT ACAATGCTCC ACTTTCCTCC
    AGCAGCCCCA TTCAGAACCC AGATCCAGTT GAAAGCACAT TGGCTGACAG CGGGGTCGGG
    ATGAATTCTT TATCCCAGGC TGCTGTCGAC ATTTTCCATT TCCAGCCCTC CGAAATCCCA
    TACCAACCAC CACCACCCCC CTACCCAACC TCCATGTTGG GTAGCTACAT CAGCCCTTCT
    CCAGCAGAGG CCATGCAAAA TAATACCACC ACCATCCAGC CACTTTCTGG AAGTCCTGGG
    ACAAGACACC ACACCATCCT TTCTGACATG GCGTCCGTTC CCAACTTTCA ACACAGATTA
    GCAAATCAGG ATGGTCCCAT ACTTGGGCGA CAGCAGGCTC CAGCTAATGG TCATGTGCCT
    GGAATTGCTG GTAGTCCAGT GCAAGGAAGA CACACTATGG TGTCCCAGGG TACACAAAGC
    AGTCCCATAC TGAGCAGACA GTATCCTGTC ACCCAGGGGA TTCAGAGTAG TCCAATTCTC
    AGCAGGCAGC CAATGGGACA AGCTGTCCAG AGCAGTCCAG TCCTCAACCG ACAGGCATCC
    CTGAATCAAG CAAACCAAAG CAGTCCAGTG TTGAGTAGAC AGCAGTCCAT TACCCTACCC
    AACCAAGGCA GCCCGGTCCT AAGCAGACAG TCCTCGCTTA GTCATCCCAG TCAGGGTAGT
    CCTGTACTAG GACGCCACCC TTCCCTAACA CAGGGTAGCC CCAGCCTGGA TAGGCATCCG
    ATGTACAGCG GTTACACCAC TCCAGAAGAG CGACACGGAG CTCTGTCACG CCAAAGCAGC
    TCTTCAGGAT ACCAGCCGCC ATCTACACCG TCTTTCCCAG TTTCTCCAGC AGGATACATC
    GAGGGAGCGG GATTCAGACA GGGGAGTCCT GCTTTCCAAC CTCAGTTGCC CGAAAAGAGA
    CGCATGTCCA GCAGTGACCG CACCAATGGT GGCCTGTCCT ATGGGACGCT CAATGGGAAG
    ATGTCCTCAC CGGTGTCCAG TGGCGGCAGC ACTCCGAGTG TTCATTTCTT CCACACACTC
    CCCGATTTCT CCAAACTCAA CATGTGTGAT GGAAGTCCAG AAACCAGAAT GAACGTGAAG
    TTTGTCCAGG ACACGTCCAA ATTCTGGTAT AAACCAGATA TCTCGAGAGA CCAAGCGATT
    AACATGCTGA AGGATCAAGA TCCTGGGGCC TTTGTGATTC GGGACAGTCA TTCATTCAGA
    GGAGCCTACG GCTTGGCTAT GAAAGTGGCT TCACCACCTC CAACCACTCA ACCTACAAAG
    AAAGCTGGAG ATGTCACTAA TGAGTTGGTG AGGCATTTTC TAATTGAGAC GAGTCCGAAG
    GGTGTGAGAC TGAAGGGCTG CCCCAACGAG CCGTACTTTG GCTGCCTGTC TGCGCTGGTC
    TACCAGCACT CCATCACTCC ACTGGCACTG CCCTGCAAAC TAGTCATACC AACCAGAGAT
    CCTCTGGAAG AGTCACCTGA GATCGCAACA CCAACCAACC CGGCGGCAGA TATGCTCAAG
    CAGGGCGCAG GGCAGAAGAG TTCAGCTGAT GGCCATGCAT GCAATGTGCT GTACATCAAC
    TCTGTGGATA TGGAGTCTTT GACTGGACCT CAAGCCATCG CCAAAGCCAT TTCAGAAACC
    ATGAACGCCA GTCCGGCACC CTCAGCCACC ATTGTTCACT TCAAGGTGTC GGCACAAGGC
    ATCACACTCA CAGACAACCA GAGAAAGCTT TTCTTCAGAC GTCATTATCC CATCGGCACA
    GTCACGTTCT GTGACATTGA TCCACAGGAG AGGAAGTGGA ACAAACCAGA GGGTGGCACT
    GCCAAATTCT TTGGCTTTGT GGCTAGAAAG CAAGGCAGCA CGACAGACAA TGTCAGTCAC
    CTGTTTGCTG AGATGGACCC AGATCAGCCG GCCACCGCCA TCGTCAACTT TGTCTCTAAA
    GTGATGATCA GCTCTCAGAA GCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334707.1
    CDS285..5711
    Translation

    Target ORF information:

    RefSeq Version XM_021479032.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479032.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
    CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA
    GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT
    TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT
    CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC
    CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG
    AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG
    AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT
    AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC
    AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG
    CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG
    ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG
    GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT
    TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT
    TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC
    AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC
    ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC
    TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG
    ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG
    TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC
    ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT
    AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA
    AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC
    CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC
    ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT
    AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC
    ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT
    GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG
    CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG
    GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT
    GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC
    AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC
    CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT
    CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA
    GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA
    GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG
    TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA
    ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA
    GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG
    TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG
    ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA
    GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC
    CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC
    TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT
    ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC
    ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA
    AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA
    TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA
    GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT
    CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG
    TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA
    GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG
    CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC
    TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT
    GGTCGATCTC CTCCAGGATT GGCTAAAACA CCACTGTCCT TTCTGGGCCT GAAGCCCCAC
    AACCCTGCTG AGATCCTGCT CAACCAGACA GGCTCAGAGA GTGAGGATGA GGAAGAGCCT
    CGTAGTTATG TGGAGTCTGT GGCGAAGGCA GCAGCAGTAC CTGCATCTCC AACATATGAC
    AGCGACAGCT TAGCTCGTCC TGCCGGACCG TCCCATGCTT ACAATGCTCC ACTTTCCTCC
    AGCAGCCCCA TTCAGAACCC AGATCCAGTT GAAAGCACAT TGGCTGACAG CGGGGTCGGG
    ATGAATTCTT TATCCCAGGC TGCTGTCGAC ATTTTCCATT TCCAGCCCTC CGAAATCCCA
    TACCAACCAC CACCACCCCC CTACCCAACC TCCATGTTGG GTAGCTACAT CAGCCCTTCT
    CCAGCAGAGG CCATGCAAAA TAATACCACC ACCATCCAGC CACTTTCTGG AAGTCCTGGG
    ACAAGACACC ACACCATCCT TTCTGACATG GCGTCCGTTC CCAACTTTCA ACACAGATTA
    GCAAATCAGG ATGGTCCCAT ACTTGGGCGA CAGCAGGCTC CAGCTAATGG TCATGTGCCT
    GGAATTGCTG GTAGTCCAGT GCAAGGAAGA CACACTATGG TGTCCCAGGG TACACAAAGC
    AGTCCCATAC TGAGCAGACA GTATCCTGTC ACCCAGGGGA TTCAGAGTAG TCCAATTCTC
    AGCAGGCAGC CAATGGGACA AGCTGTCCAG AGCAGTCCAG TCCTCAACCG ACAGGCATCC
    CTGAATCAAG CAAACCAAAG CAGTCCAGTG TTGAGTAGAC AGCAGTCCAT TACCCTACCC
    AACCAAGGCA GCCCGGTCCT AAGCAGACAG TCCTCGCTTA GTCATCCCAG TCAGGGTAGT
    CCTGTACTAG GACGCCACCC TTCCCTAACA CAGGGTAGCC CCAGCCTGGA TAGGCATCCG
    ATGTACAGCG GTTACACCAC TCCAGAAGAG CGACACGGAG CTCTGTCACG CCAAAGCAGC
    TCTTCAGGAT ACCAGCCGCC ATCTACACCG TCTTTCCCAG TTTCTCCAGC AGGATACATC
    GAGGGAGCGG GATTCAGACA GGGGAGTCCT GCTTTCCAAC CTCAGTTGCC CGAAAAGAGA
    CGCATGTCCA GCAGTGACCG CACCAATGGT GGCCTGTCCT ATGGGACGCT CAATGGGAAG
    ATGTCCTCAC CGGTGTCCAG TGGCGGCAGC ACTCCGAGTG TTCATTTCTT CCACACACTC
    CCCGATTTCT CCAAACTCAA CATGTGTGAT GGAAGTCCAG AAACCAGAAT GAACGTGAAG
    TTTGTCCAGG ACACGTCCAA ATTCTGGTAT AAACCAGATA TCTCGAGAGA CCAAGCGATT
    AACATGCTGA AGGATCAAGA TCCTGGGGCC TTTGTGATTC GGGACAGTCA TTCATTCAGA
    GGAGCCTACG GCTTGGCTAT GAAAGTGGCT TCACCACCTC CAACCACTCA ACCTACAAAG
    AAAGCTGGAG ATGTCACTAA TGAGTTGGTG AGGCATTTTC TAATTGAGAC GAGTCCGAAG
    GGTGTGAGAC TGAAGGGCTG CCCCAACGAG CCGTACTTTG GCTGCCTGTC TGCGCTGGTC
    TACCAGCACT CCATCACTCC ACTGGCACTG CCCTGCAAAC TAGTCATACC AACCAGAGAT
    CCTCTGGAAG AGTCACCTGA GATCGCAACA CCAACCAACC CGGCGGCAGA TATGCTCAAG
    CAGGGCGCAG GGCAGAAGAG TTCAGCTGAT GGCCATGCAT GCAATGTGCT GTACATCAAC
    TCTGTGGATA TGGAGTCTTT GACTGGACCT CAAGCCATCG CCAAAGCCAT TTCAGAAACC
    ATGAACGCCA GTCCGGCACC CTCAGCCACC ATTGTTCACT TCAAGGTGTC GGCACAAGGC
    ATCACACTCA CAGACAACCA GAGAAAGCTT TTCTTCAGAC GTCATTATCC CATCGGCACA
    GTCACGTTCT GTGACATTGA TCCACAGGAG AGGAAGTGGA ACAAACCAGA GGGTGGCACT
    GCCAAATTCT TTGGCTTTGT GGCTAGAAAG CAAGGCAGCA CGACAGACAA TGTCAGTCAC
    CTGTTTGCTG AGATGGACCC AGATCAGCCG GCCACCGCCA TCGTCAACTT TGTCTCTAAA
    GTGATGATCA GCTCTCAGAA GCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144119
    Clone ID Related Accession (Same CDS sequence) XM_021479033.1
    Accession Version XM_021479033.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5409bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product tensin-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
    CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA
    GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT
    TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT
    CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC
    CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG
    AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG
    AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT
    AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC
    AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG
    CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG
    ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG
    GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT
    TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT
    TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC
    AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC
    ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC
    TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG
    ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG
    TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC
    ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT
    AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA
    AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC
    CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC
    ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT
    AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC
    ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT
    GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG
    CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG
    GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT
    GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC
    AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC
    CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT
    CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA
    GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA
    GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG
    TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA
    ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA
    GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG
    TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG
    ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA
    GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC
    CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC
    TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT
    ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC
    ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA
    AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA
    TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA
    GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT
    CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG
    TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA
    GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG
    CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC
    TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT
    GGTCGATCTC CTCCAGGATT GGCTAAAACA CCACTGTCCT TTCTGGGCCT GAAGCCCCAC
    AACCCTGCTG AGATCCTGCT CAACCAGACA GGCTCAGAGC CTCGTAGTTA TGTGGAGTCT
    GTGGCGAAGG CAGCAGCAGT ACCTGCATCT CCAACATATG ACAGCGACAG CTTAGCTCGT
    CCTGCCGGAC CGTCCCATGC TTACAATGCT CCACTTTCCT CCAGCAGCCC CATTCAGAAC
    CCAGATCCAG TTGAAAGCAC ATTGGCTGAC AGCGGGGTCG GGATGAATTC TTTATCCCAG
    GCTGCTGTCG ACATTTTCCA TTTCCAGCCC TCCGAAATCC CATACCAACC ACCACCACCC
    CCCTACCCAA CCTCCATGTT GGGTAGCTAC ATCAGCCCTT CTCCAGCAGA GGCCATGCAA
    AATAATACCA CCACCATCCA GCCACTTTCT GGAAGTCCTG GGACAAGACA CCACACCATC
    CTTTCTGACA TGGCGTCCGT TCCCAACTTT CAACACAGAT TAGCAAATCA GGATGGTCCC
    ATACTTGGGC GACAGCAGGC TCCAGCTAAT GGTCATGTGC CTGGAATTGC TGGTAGTCCA
    GTGCAAGGAA GACACACTAT GGTGTCCCAG GGTACACAAA GCAGTCCCAT ACTGAGCAGA
    CAGTATCCTG TCACCCAGGG GATTCAGAGT AGTCCAATTC TCAGCAGGCA GCCAATGGGA
    CAAGCTGTCC AGAGCAGTCC AGTCCTCAAC CGACAGGCAT CCCTGAATCA AGCAAACCAA
    AGCAGTCCAG TGTTGAGTAG ACAGCAGTCC ATTACCCTAC CCAACCAAGG CAGCCCGGTC
    CTAAGCAGAC AGTCCTCGCT TAGTCATCCC AGTCAGGGTA GTCCTGTACT AGGACGCCAC
    CCTTCCCTAA CACAGGGTAG CCCCAGCCTG GATAGGCATC CGATGTACAG CGGTTACACC
    ACTCCAGAAG AGCGACACGG AGCTCTGTCA CGCCAAAGCA GCTCTTCAGG ATACCAGCCG
    CCATCTACAC CGTCTTTCCC AGTTTCTCCA GCAGGATACA TCGAGGGAGC GGGATTCAGA
    CAGGGGAGTC CTGCTTTCCA ACCTCAGTTG CCCGAAAAGA GACGCATGTC CAGCAGTGAC
    CGCACCAATG GTGGCCTGTC CTATGGGACG CTCAATGGGA AGATGTCCTC ACCGGTGTCC
    AGTGGCGGCA GCACTCCGAG TGTTCATTTC TTCCACACAC TCCCCGATTT CTCCAAACTC
    AACATGTGTG ATGGAAGTCC AGAAACCAGA ATGAACGTGA AGTTTGTCCA GGACACGTCC
    AAATTCTGGT ATAAACCAGA TATCTCGAGA GACCAAGCGA TTAACATGCT GAAGGATCAA
    GATCCTGGGG CCTTTGTGAT TCGGGACAGT CATTCATTCA GAGGAGCCTA CGGCTTGGCT
    ATGAAAGTGG CTTCACCACC TCCAACCACT CAACCTACAA AGAAAGCTGG AGATGTCACT
    AATGAGTTGG TGAGGCATTT TCTAATTGAG ACGAGTCCGA AGGGTGTGAG ACTGAAGGGC
    TGCCCCAACG AGCCGTACTT TGGCTGCCTG TCTGCGCTGG TCTACCAGCA CTCCATCACT
    CCACTGGCAC TGCCCTGCAA ACTAGTCATA CCAACCAGAG ATCCTCTGGA AGAGTCACCT
    GAGATCGCAA CACCAACCAA CCCGGCGGCA GATATGCTCA AGCAGGGCGC AGGGCAGAAG
    AGTTCAGCTG ATGGCCATGC ATGCAATGTG CTGTACATCA ACTCTGTGGA TATGGAGTCT
    TTGACTGGAC CTCAAGCCAT CGCCAAAGCC ATTTCAGAAA CCATGAACGC CAGTCCGGCA
    CCCTCAGCCA CCATTGTTCA CTTCAAGGTG TCGGCACAAG GCATCACACT CACAGACAAC
    CAGAGAAAGC TTTTCTTCAG ACGTCATTAT CCCATCGGCA CAGTCACGTT CTGTGACATT
    GATCCACAGG AGAGGAAGTG GAACAAACCA GAGGGTGGCA CTGCCAAATT CTTTGGCTTT
    GTGGCTAGAA AGCAAGGCAG CACGACAGAC AATGTCAGTC ACCTGTTTGC TGAGATGGAC
    CCAGATCAGC CGGCCACCGC CATCGTCAAC TTTGTCTCTA AAGTGATGAT CAGCTCTCAG
    AAGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334708.1
    CDS284..5692
    Translation

    Target ORF information:

    RefSeq Version XM_021479033.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479033.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
    CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA
    GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT
    TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT
    CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC
    CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG
    AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG
    AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT
    AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC
    AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG
    CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG
    ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG
    GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT
    TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT
    TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC
    AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC
    ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC
    TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG
    ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG
    TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC
    ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT
    AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA
    AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC
    CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC
    ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT
    AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC
    ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT
    GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG
    CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG
    GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT
    GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC
    AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC
    CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT
    CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA
    GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA
    GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG
    TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA
    ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA
    GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG
    TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG
    ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA
    GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC
    CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC
    TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT
    ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC
    ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA
    AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA
    TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA
    GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT
    CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG
    TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA
    GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG
    CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC
    TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT
    GGTCGATCTC CTCCAGGATT GGCTAAAACA CCACTGTCCT TTCTGGGCCT GAAGCCCCAC
    AACCCTGCTG AGATCCTGCT CAACCAGACA GGCTCAGAGC CTCGTAGTTA TGTGGAGTCT
    GTGGCGAAGG CAGCAGCAGT ACCTGCATCT CCAACATATG ACAGCGACAG CTTAGCTCGT
    CCTGCCGGAC CGTCCCATGC TTACAATGCT CCACTTTCCT CCAGCAGCCC CATTCAGAAC
    CCAGATCCAG TTGAAAGCAC ATTGGCTGAC AGCGGGGTCG GGATGAATTC TTTATCCCAG
    GCTGCTGTCG ACATTTTCCA TTTCCAGCCC TCCGAAATCC CATACCAACC ACCACCACCC
    CCCTACCCAA CCTCCATGTT GGGTAGCTAC ATCAGCCCTT CTCCAGCAGA GGCCATGCAA
    AATAATACCA CCACCATCCA GCCACTTTCT GGAAGTCCTG GGACAAGACA CCACACCATC
    CTTTCTGACA TGGCGTCCGT TCCCAACTTT CAACACAGAT TAGCAAATCA GGATGGTCCC
    ATACTTGGGC GACAGCAGGC TCCAGCTAAT GGTCATGTGC CTGGAATTGC TGGTAGTCCA
    GTGCAAGGAA GACACACTAT GGTGTCCCAG GGTACACAAA GCAGTCCCAT ACTGAGCAGA
    CAGTATCCTG TCACCCAGGG GATTCAGAGT AGTCCAATTC TCAGCAGGCA GCCAATGGGA
    CAAGCTGTCC AGAGCAGTCC AGTCCTCAAC CGACAGGCAT CCCTGAATCA AGCAAACCAA
    AGCAGTCCAG TGTTGAGTAG ACAGCAGTCC ATTACCCTAC CCAACCAAGG CAGCCCGGTC
    CTAAGCAGAC AGTCCTCGCT TAGTCATCCC AGTCAGGGTA GTCCTGTACT AGGACGCCAC
    CCTTCCCTAA CACAGGGTAG CCCCAGCCTG GATAGGCATC CGATGTACAG CGGTTACACC
    ACTCCAGAAG AGCGACACGG AGCTCTGTCA CGCCAAAGCA GCTCTTCAGG ATACCAGCCG
    CCATCTACAC CGTCTTTCCC AGTTTCTCCA GCAGGATACA TCGAGGGAGC GGGATTCAGA
    CAGGGGAGTC CTGCTTTCCA ACCTCAGTTG CCCGAAAAGA GACGCATGTC CAGCAGTGAC
    CGCACCAATG GTGGCCTGTC CTATGGGACG CTCAATGGGA AGATGTCCTC ACCGGTGTCC
    AGTGGCGGCA GCACTCCGAG TGTTCATTTC TTCCACACAC TCCCCGATTT CTCCAAACTC
    AACATGTGTG ATGGAAGTCC AGAAACCAGA ATGAACGTGA AGTTTGTCCA GGACACGTCC
    AAATTCTGGT ATAAACCAGA TATCTCGAGA GACCAAGCGA TTAACATGCT GAAGGATCAA
    GATCCTGGGG CCTTTGTGAT TCGGGACAGT CATTCATTCA GAGGAGCCTA CGGCTTGGCT
    ATGAAAGTGG CTTCACCACC TCCAACCACT CAACCTACAA AGAAAGCTGG AGATGTCACT
    AATGAGTTGG TGAGGCATTT TCTAATTGAG ACGAGTCCGA AGGGTGTGAG ACTGAAGGGC
    TGCCCCAACG AGCCGTACTT TGGCTGCCTG TCTGCGCTGG TCTACCAGCA CTCCATCACT
    CCACTGGCAC TGCCCTGCAA ACTAGTCATA CCAACCAGAG ATCCTCTGGA AGAGTCACCT
    GAGATCGCAA CACCAACCAA CCCGGCGGCA GATATGCTCA AGCAGGGCGC AGGGCAGAAG
    AGTTCAGCTG ATGGCCATGC ATGCAATGTG CTGTACATCA ACTCTGTGGA TATGGAGTCT
    TTGACTGGAC CTCAAGCCAT CGCCAAAGCC ATTTCAGAAA CCATGAACGC CAGTCCGGCA
    CCCTCAGCCA CCATTGTTCA CTTCAAGGTG TCGGCACAAG GCATCACACT CACAGACAAC
    CAGAGAAAGC TTTTCTTCAG ACGTCATTAT CCCATCGGCA CAGTCACGTT CTGTGACATT
    GATCCACAGG AGAGGAAGTG GAACAAACCA GAGGGTGGCA CTGCCAAATT CTTTGGCTTT
    GTGGCTAGAA AGCAAGGCAG CACGACAGAC AATGTCAGTC ACCTGTTTGC TGAGATGGAC
    CCAGATCAGC CGGCCACCGC CATCGTCAAC TTTGTCTCTA AAGTGATGAT CAGCTCTCAG
    AAGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144120
    Clone ID Related Accession (Same CDS sequence) XM_021479034.1
    Accession Version XM_021479034.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5406bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product tensin-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
    CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA
    GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT
    TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT
    CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC
    CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG
    AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG
    AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT
    AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC
    AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG
    CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG
    ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG
    GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT
    TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT
    TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC
    AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC
    ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC
    TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG
    ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG
    TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC
    ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT
    AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA
    AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC
    CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC
    ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT
    AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC
    ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT
    GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG
    CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG
    GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT
    GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC
    AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC
    CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT
    CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA
    GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA
    GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG
    TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA
    ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA
    GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG
    TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG
    ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA
    GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC
    CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC
    TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT
    ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC
    ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA
    AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA
    TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA
    GCTCACCGTG TAGCTGGACT TCCATCTGGT GAAATGTCGC CAGATGAGGC TGCAGTTCCT
    GGACGCTGTC GGACCCTTAG TGAGGGCCTT TCCCAAGACG AAAGCCCAAC TCGAGGAAGC
    TCAGTCCGCT CCCCCATTCG CTGCATCTCA CCAGAGCTGG CCAATACAAT TGCTCTGAAC
    CCTGGGGGGC GGCCCAAGGA GAGGCACATG CACAGCTACA GAGAAGCGTT TGAGGAAATG
    GATGGGGGTC CCGTTAGCCC CCCTCCAATT GTTGGTGGTG AGGTCCTACC CCAAACCCCT
    GCCTTCCCTG TCTCGCCACA AACCCCTTAC TTCAACATGT GTCGATCTCC TCCAGGATTG
    GCTAAAACAC CACTGTCCTT TCTGGGCCTG AAGCCCCACA ACCCTGCTGA GATCCTGCTC
    AACCAGACAG GCTCAGAGAG TGAGGATGAG GAAGAGCCTC GTAGTTATGT GGAGTCTGTG
    GCGAAGGCAG CAGCAGTACC TGCATCTCCA ACATATGACA GCGACAGCTT AGCTCGTCCT
    GCCGGACCGT CCCATGCTTA CAATGCTCCA CTTTCCTCCA GCAGCCCCAT TCAGAACCCA
    GATCCAGTTG AAAGCACATT GGCTGACAGC GGGGTCGGGA TGAATTCTTT ATCCCAGGCT
    GCTGTCGACA TTTTCCATTT CCAGCCCTCC GAAATCCCAT ACCAACCACC ACCACCCCCC
    TACCCAACCT CCATGTTGGG TAGCTACATC AGCCCTTCTC CAGCAGAGGC CATGCAAAAT
    AATACCACCA CCATCCAGCC ACTTTCTGGA AGTCCTGGGA CAAGACACCA CACCATCCTT
    TCTGACATGG CGTCCGTTCC CAACTTTCAA CACAGATTAG CAAATCAGGA TGGTCCCATA
    CTTGGGCGAC AGCAGGCTCC AGCTAATGGT CATGTGCCTG GAATTGCTGG TAGTCCAGTG
    CAAGGAAGAC ACACTATGGT GTCCCAGGGT ACACAAAGCA GTCCCATACT GAGCAGACAG
    TATCCTGTCA CCCAGGGGAT TCAGAGTAGT CCAATTCTCA GCAGGCAGCC AATGGGACAA
    GCTGTCCAGA GCAGTCCAGT CCTCAACCGA CAGGCATCCC TGAATCAAGC AAACCAAAGC
    AGTCCAGTGT TGAGTAGACA GCAGTCCATT ACCCTACCCA ACCAAGGCAG CCCGGTCCTA
    AGCAGACAGT CCTCGCTTAG TCATCCCAGT CAGGGTAGTC CTGTACTAGG ACGCCACCCT
    TCCCTAACAC AGGGTAGCCC CAGCCTGGAT AGGCATCCGA TGTACAGCGG TTACACCACT
    CCAGAAGAGC GACACGGAGC TCTGTCACGC CAAAGCAGCT CTTCAGGATA CCAGCCGCCA
    TCTACACCGT CTTTCCCAGT TTCTCCAGCA GGATACATCG AGGGAGCGGG ATTCAGACAG
    GGGAGTCCTG CTTTCCAACC TCAGTTGCCC GAAAAGAGAC GCATGTCCAG CAGTGACCGC
    ACCAATGGTG GCCTGTCCTA TGGGACGCTC AATGGGAAGA TGTCCTCACC GGTGTCCAGT
    GGCGGCAGCA CTCCGAGTGT TCATTTCTTC CACACACTCC CCGATTTCTC CAAACTCAAC
    ATGTGTGATG GAAGTCCAGA AACCAGAATG AACGTGAAGT TTGTCCAGGA CACGTCCAAA
    TTCTGGTATA AACCAGATAT CTCGAGAGAC CAAGCGATTA ACATGCTGAA GGATCAAGAT
    CCTGGGGCCT TTGTGATTCG GGACAGTCAT TCATTCAGAG GAGCCTACGG CTTGGCTATG
    AAAGTGGCTT CACCACCTCC AACCACTCAA CCTACAAAGA AAGCTGGAGA TGTCACTAAT
    GAGTTGGTGA GGCATTTTCT AATTGAGACG AGTCCGAAGG GTGTGAGACT GAAGGGCTGC
    CCCAACGAGC CGTACTTTGG CTGCCTGTCT GCGCTGGTCT ACCAGCACTC CATCACTCCA
    CTGGCACTGC CCTGCAAACT AGTCATACCA ACCAGAGATC CTCTGGAAGA GTCACCTGAG
    ATCGCAACAC CAACCAACCC GGCGGCAGAT ATGCTCAAGC AGGGCGCAGG GCAGAAGAGT
    TCAGCTGATG GCCATGCATG CAATGTGCTG TACATCAACT CTGTGGATAT GGAGTCTTTG
    ACTGGACCTC AAGCCATCGC CAAAGCCATT TCAGAAACCA TGAACGCCAG TCCGGCACCC
    TCAGCCACCA TTGTTCACTT CAAGGTGTCG GCACAAGGCA TCACACTCAC AGACAACCAG
    AGAAAGCTTT TCTTCAGACG TCATTATCCC ATCGGCACAG TCACGTTCTG TGACATTGAT
    CCACAGGAGA GGAAGTGGAA CAAACCAGAG GGTGGCACTG CCAAATTCTT TGGCTTTGTG
    GCTAGAAAGC AAGGCAGCAC GACAGACAAT GTCAGTCACC TGTTTGCTGA GATGGACCCA
    GATCAGCCGG CCACCGCCAT CGTCAACTTT GTCTCTAAAG TGATGATCAG CTCTCAGAAG
    CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334709.1
    CDS284..5689
    Translation

    Target ORF information:

    RefSeq Version XM_021479034.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479034.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
    CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA
    GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT
    TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT
    CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC
    CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG
    AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG
    AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT
    AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC
    AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG
    CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG
    ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG
    GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT
    TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT
    TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC
    AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC
    ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC
    TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG
    ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG
    TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC
    ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT
    AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA
    AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC
    CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC
    ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT
    AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC
    ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT
    GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG
    CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG
    GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT
    GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC
    AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC
    CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT
    CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA
    GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA
    GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG
    TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA
    ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA
    GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG
    TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG
    ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA
    GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC
    CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC
    TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT
    ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC
    ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA
    AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA
    TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA
    GCTCACCGTG TAGCTGGACT TCCATCTGGT GAAATGTCGC CAGATGAGGC TGCAGTTCCT
    GGACGCTGTC GGACCCTTAG TGAGGGCCTT TCCCAAGACG AAAGCCCAAC TCGAGGAAGC
    TCAGTCCGCT CCCCCATTCG CTGCATCTCA CCAGAGCTGG CCAATACAAT TGCTCTGAAC
    CCTGGGGGGC GGCCCAAGGA GAGGCACATG CACAGCTACA GAGAAGCGTT TGAGGAAATG
    GATGGGGGTC CCGTTAGCCC CCCTCCAATT GTTGGTGGTG AGGTCCTACC CCAAACCCCT
    GCCTTCCCTG TCTCGCCACA AACCCCTTAC TTCAACATGT GTCGATCTCC TCCAGGATTG
    GCTAAAACAC CACTGTCCTT TCTGGGCCTG AAGCCCCACA ACCCTGCTGA GATCCTGCTC
    AACCAGACAG GCTCAGAGAG TGAGGATGAG GAAGAGCCTC GTAGTTATGT GGAGTCTGTG
    GCGAAGGCAG CAGCAGTACC TGCATCTCCA ACATATGACA GCGACAGCTT AGCTCGTCCT
    GCCGGACCGT CCCATGCTTA CAATGCTCCA CTTTCCTCCA GCAGCCCCAT TCAGAACCCA
    GATCCAGTTG AAAGCACATT GGCTGACAGC GGGGTCGGGA TGAATTCTTT ATCCCAGGCT
    GCTGTCGACA TTTTCCATTT CCAGCCCTCC GAAATCCCAT ACCAACCACC ACCACCCCCC
    TACCCAACCT CCATGTTGGG TAGCTACATC AGCCCTTCTC CAGCAGAGGC CATGCAAAAT
    AATACCACCA CCATCCAGCC ACTTTCTGGA AGTCCTGGGA CAAGACACCA CACCATCCTT
    TCTGACATGG CGTCCGTTCC CAACTTTCAA CACAGATTAG CAAATCAGGA TGGTCCCATA
    CTTGGGCGAC AGCAGGCTCC AGCTAATGGT CATGTGCCTG GAATTGCTGG TAGTCCAGTG
    CAAGGAAGAC ACACTATGGT GTCCCAGGGT ACACAAAGCA GTCCCATACT GAGCAGACAG
    TATCCTGTCA CCCAGGGGAT TCAGAGTAGT CCAATTCTCA GCAGGCAGCC AATGGGACAA
    GCTGTCCAGA GCAGTCCAGT CCTCAACCGA CAGGCATCCC TGAATCAAGC AAACCAAAGC
    AGTCCAGTGT TGAGTAGACA GCAGTCCATT ACCCTACCCA ACCAAGGCAG CCCGGTCCTA
    AGCAGACAGT CCTCGCTTAG TCATCCCAGT CAGGGTAGTC CTGTACTAGG ACGCCACCCT
    TCCCTAACAC AGGGTAGCCC CAGCCTGGAT AGGCATCCGA TGTACAGCGG TTACACCACT
    CCAGAAGAGC GACACGGAGC TCTGTCACGC CAAAGCAGCT CTTCAGGATA CCAGCCGCCA
    TCTACACCGT CTTTCCCAGT TTCTCCAGCA GGATACATCG AGGGAGCGGG ATTCAGACAG
    GGGAGTCCTG CTTTCCAACC TCAGTTGCCC GAAAAGAGAC GCATGTCCAG CAGTGACCGC
    ACCAATGGTG GCCTGTCCTA TGGGACGCTC AATGGGAAGA TGTCCTCACC GGTGTCCAGT
    GGCGGCAGCA CTCCGAGTGT TCATTTCTTC CACACACTCC CCGATTTCTC CAAACTCAAC
    ATGTGTGATG GAAGTCCAGA AACCAGAATG AACGTGAAGT TTGTCCAGGA CACGTCCAAA
    TTCTGGTATA AACCAGATAT CTCGAGAGAC CAAGCGATTA ACATGCTGAA GGATCAAGAT
    CCTGGGGCCT TTGTGATTCG GGACAGTCAT TCATTCAGAG GAGCCTACGG CTTGGCTATG
    AAAGTGGCTT CACCACCTCC AACCACTCAA CCTACAAAGA AAGCTGGAGA TGTCACTAAT
    GAGTTGGTGA GGCATTTTCT AATTGAGACG AGTCCGAAGG GTGTGAGACT GAAGGGCTGC
    CCCAACGAGC CGTACTTTGG CTGCCTGTCT GCGCTGGTCT ACCAGCACTC CATCACTCCA
    CTGGCACTGC CCTGCAAACT AGTCATACCA ACCAGAGATC CTCTGGAAGA GTCACCTGAG
    ATCGCAACAC CAACCAACCC GGCGGCAGAT ATGCTCAAGC AGGGCGCAGG GCAGAAGAGT
    TCAGCTGATG GCCATGCATG CAATGTGCTG TACATCAACT CTGTGGATAT GGAGTCTTTG
    ACTGGACCTC AAGCCATCGC CAAAGCCATT TCAGAAACCA TGAACGCCAG TCCGGCACCC
    TCAGCCACCA TTGTTCACTT CAAGGTGTCG GCACAAGGCA TCACACTCAC AGACAACCAG
    AGAAAGCTTT TCTTCAGACG TCATTATCCC ATCGGCACAG TCACGTTCTG TGACATTGAT
    CCACAGGAGA GGAAGTGGAA CAAACCAGAG GGTGGCACTG CCAAATTCTT TGGCTTTGTG
    GCTAGAAAGC AAGGCAGCAC GACAGACAAT GTCAGTCACC TGTTTGCTGA GATGGACCCA
    GATCAGCCGG CCACCGCCAT CGTCAACTTT GTCTCTAAAG TGATGATCAG CTCTCAGAAG
    CGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144121
    Clone ID Related Accession (Same CDS sequence) XM_021479035.1
    Accession Version XM_021479035.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product tensin-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
    CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA
    GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT
    TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT
    CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC
    CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG
    AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG
    AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT
    AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC
    AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG
    CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG
    ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG
    GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT
    TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT
    TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC
    AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC
    ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC
    TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG
    ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG
    TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC
    ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT
    AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA
    AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC
    CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC
    ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT
    AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC
    ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT
    GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG
    CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG
    GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT
    GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC
    AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC
    CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT
    CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA
    GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA
    GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG
    TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA
    ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA
    GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG
    TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG
    ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA
    GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC
    CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC
    TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT
    ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC
    ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA
    AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA
    TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA
    GCTCACCGTG TAGCTGGACT TCCATCTGGT GAAATGTCGC CAGATGAGGC TGCAGTTCCT
    GGACGCTGTC GGACCCTTAG TGAGGGCCTT TCCCAAGACG AAAGCCCAAC TCGAGGAAGC
    TCAGTCCGCT CCCCCATTCG CTGCATCTCA CCAGAGCTGG CCAATACAAT TGCTCTGAAC
    CCTGGGGGGC GGCCCAAGGA GAGGCACATG CACAGCTACA GAGAAGCGTT TGAGGAAATG
    GATGGGGGTC CCGTTAGCCC CCCTCCAATT GTTGGTGGTC GATCTCCTCC AGGATTGGCT
    AAAACACCAC TGTCCTTTCT GGGCCTGAAG CCCCACAACC CTGCTGAGAT CCTGCTCAAC
    CAGACAGGCT CAGAGCCTCG TAGTTATGTG GAGTCTGTGG CGAAGGCAGC AGCAGTACCT
    GCATCTCCAA CATATGACAG CGACAGCTTA GCTCGTCCTG CCGGACCGTC CCATGCTTAC
    AATGCTCCAC TTTCCTCCAG CAGCCCCATT CAGAACCCAG ATCCAGTTGA AAGCACATTG
    GCTGACAGCG GGGTCGGGAT GAATTCTTTA TCCCAGGCTG CTGTCGACAT TTTCCATTTC
    CAGCCCTCCG AAATCCCATA CCAACCACCA CCACCCCCCT ACCCAACCTC CATGTTGGGT
    AGCTACATCA GCCCTTCTCC AGCAGAGGCC ATGCAAAATA ATACCACCAC CATCCAGCCA
    CTTTCTGGAA GTCCTGGGAC AAGACACCAC ACCATCCTTT CTGACATGGC GTCCGTTCCC
    AACTTTCAAC ACAGATTAGC AAATCAGGAT GGTCCCATAC TTGGGCGACA GCAGGCTCCA
    GCTAATGGTC ATGTGCCTGG AATTGCTGGT AGTCCAGTGC AAGGAAGACA CACTATGGTG
    TCCCAGGGTA CACAAAGCAG TCCCATACTG AGCAGACAGT ATCCTGTCAC CCAGGGGATT
    CAGAGTAGTC CAATTCTCAG CAGGCAGCCA ATGGGACAAG CTGTCCAGAG CAGTCCAGTC
    CTCAACCGAC AGGCATCCCT GAATCAAGCA AACCAAAGCA GTCCAGTGTT GAGTAGACAG
    CAGTCCATTA CCCTACCCAA CCAAGGCAGC CCGGTCCTAA GCAGACAGTC CTCGCTTAGT
    CATCCCAGTC AGGGTAGTCC TGTACTAGGA CGCCACCCTT CCCTAACACA GGGTAGCCCC
    AGCCTGGATA GGCATCCGAT GTACAGCGGT TACACCACTC CAGAAGAGCG ACACGGAGCT
    CTGTCACGCC AAAGCAGCTC TTCAGGATAC CAGCCGCCAT CTACACCGTC TTTCCCAGTT
    TCTCCAGCAG GATACATCGA GGGAGCGGGA TTCAGACAGG GGAGTCCTGC TTTCCAACCT
    CAGTTGCCCG AAAAGAGACG CATGTCCAGC AGTGACCGCA CCAATGGTGG CCTGTCCTAT
    GGGACGCTCA ATGGGAAGAT GTCCTCACCG GTGTCCAGTG GCGGCAGCAC TCCGAGTGTT
    CATTTCTTCC ACACACTCCC CGATTTCTCC AAACTCAACA TGTGTGATGG AAGTCCAGAA
    ACCAGAATGA ACGTGAAGTT TGTCCAGGAC ACGTCCAAAT TCTGGTATAA ACCAGATATC
    TCGAGAGACC AAGCGATTAA CATGCTGAAG GATCAAGATC CTGGGGCCTT TGTGATTCGG
    GACAGTCATT CATTCAGAGG AGCCTACGGC TTGGCTATGA AAGTGGCTTC ACCACCTCCA
    ACCACTCAAC CTACAAAGAA AGCTGGAGAT GTCACTAATG AGTTGGTGAG GCATTTTCTA
    ATTGAGACGA GTCCGAAGGG TGTGAGACTG AAGGGCTGCC CCAACGAGCC GTACTTTGGC
    TGCCTGTCTG CGCTGGTCTA CCAGCACTCC ATCACTCCAC TGGCACTGCC CTGCAAACTA
    GTCATACCAA CCAGAGATCC TCTGGAAGAG TCACCTGAGA TCGCAACACC AACCAACCCG
    GCGGCAGATA TGCTCAAGCA GGGCGCAGGG CAGAAGAGTT CAGCTGATGG CCATGCATGC
    AATGTGCTGT ACATCAACTC TGTGGATATG GAGTCTTTGA CTGGACCTCA AGCCATCGCC
    AAAGCCATTT CAGAAACCAT GAACGCCAGT CCGGCACCCT CAGCCACCAT TGTTCACTTC
    AAGGTGTCGG CACAAGGCAT CACACTCACA GACAACCAGA GAAAGCTTTT CTTCAGACGT
    CATTATCCCA TCGGCACAGT CACGTTCTGT GACATTGATC CACAGGAGAG GAAGTGGAAC
    AAACCAGAGG GTGGCACTGC CAAATTCTTT GGCTTTGTGG CTAGAAAGCA AGGCAGCACG
    ACAGACAATG TCAGTCACCT GTTTGCTGAG ATGGACCCAG ATCAGCCGGC CACCGCCATC
    GTCAACTTTG TCTCTAAAGT GATGATCAGC TCTCAGAAGC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334710.1
    CDS283..5607
    Translation

    Target ORF information:

    RefSeq Version XM_021479035.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479035.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
    CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA
    GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT
    TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT
    CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC
    CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG
    AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG
    AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT
    AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC
    AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG
    CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG
    ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG
    GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT
    TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT
    TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC
    AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC
    ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC
    TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG
    ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG
    TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC
    ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT
    AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA
    AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC
    CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC
    ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT
    AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC
    ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT
    GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG
    CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG
    GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT
    GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC
    AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC
    CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT
    CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA
    GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA
    GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG
    TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA
    ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA
    GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG
    TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG
    ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA
    GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC
    CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC
    TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT
    ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC
    ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA
    AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA
    TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA
    GCTCACCGTG TAGCTGGACT TCCATCTGGT GAAATGTCGC CAGATGAGGC TGCAGTTCCT
    GGACGCTGTC GGACCCTTAG TGAGGGCCTT TCCCAAGACG AAAGCCCAAC TCGAGGAAGC
    TCAGTCCGCT CCCCCATTCG CTGCATCTCA CCAGAGCTGG CCAATACAAT TGCTCTGAAC
    CCTGGGGGGC GGCCCAAGGA GAGGCACATG CACAGCTACA GAGAAGCGTT TGAGGAAATG
    GATGGGGGTC CCGTTAGCCC CCCTCCAATT GTTGGTGGTC GATCTCCTCC AGGATTGGCT
    AAAACACCAC TGTCCTTTCT GGGCCTGAAG CCCCACAACC CTGCTGAGAT CCTGCTCAAC
    CAGACAGGCT CAGAGCCTCG TAGTTATGTG GAGTCTGTGG CGAAGGCAGC AGCAGTACCT
    GCATCTCCAA CATATGACAG CGACAGCTTA GCTCGTCCTG CCGGACCGTC CCATGCTTAC
    AATGCTCCAC TTTCCTCCAG CAGCCCCATT CAGAACCCAG ATCCAGTTGA AAGCACATTG
    GCTGACAGCG GGGTCGGGAT GAATTCTTTA TCCCAGGCTG CTGTCGACAT TTTCCATTTC
    CAGCCCTCCG AAATCCCATA CCAACCACCA CCACCCCCCT ACCCAACCTC CATGTTGGGT
    AGCTACATCA GCCCTTCTCC AGCAGAGGCC ATGCAAAATA ATACCACCAC CATCCAGCCA
    CTTTCTGGAA GTCCTGGGAC AAGACACCAC ACCATCCTTT CTGACATGGC GTCCGTTCCC
    AACTTTCAAC ACAGATTAGC AAATCAGGAT GGTCCCATAC TTGGGCGACA GCAGGCTCCA
    GCTAATGGTC ATGTGCCTGG AATTGCTGGT AGTCCAGTGC AAGGAAGACA CACTATGGTG
    TCCCAGGGTA CACAAAGCAG TCCCATACTG AGCAGACAGT ATCCTGTCAC CCAGGGGATT
    CAGAGTAGTC CAATTCTCAG CAGGCAGCCA ATGGGACAAG CTGTCCAGAG CAGTCCAGTC
    CTCAACCGAC AGGCATCCCT GAATCAAGCA AACCAAAGCA GTCCAGTGTT GAGTAGACAG
    CAGTCCATTA CCCTACCCAA CCAAGGCAGC CCGGTCCTAA GCAGACAGTC CTCGCTTAGT
    CATCCCAGTC AGGGTAGTCC TGTACTAGGA CGCCACCCTT CCCTAACACA GGGTAGCCCC
    AGCCTGGATA GGCATCCGAT GTACAGCGGT TACACCACTC CAGAAGAGCG ACACGGAGCT
    CTGTCACGCC AAAGCAGCTC TTCAGGATAC CAGCCGCCAT CTACACCGTC TTTCCCAGTT
    TCTCCAGCAG GATACATCGA GGGAGCGGGA TTCAGACAGG GGAGTCCTGC TTTCCAACCT
    CAGTTGCCCG AAAAGAGACG CATGTCCAGC AGTGACCGCA CCAATGGTGG CCTGTCCTAT
    GGGACGCTCA ATGGGAAGAT GTCCTCACCG GTGTCCAGTG GCGGCAGCAC TCCGAGTGTT
    CATTTCTTCC ACACACTCCC CGATTTCTCC AAACTCAACA TGTGTGATGG AAGTCCAGAA
    ACCAGAATGA ACGTGAAGTT TGTCCAGGAC ACGTCCAAAT TCTGGTATAA ACCAGATATC
    TCGAGAGACC AAGCGATTAA CATGCTGAAG GATCAAGATC CTGGGGCCTT TGTGATTCGG
    GACAGTCATT CATTCAGAGG AGCCTACGGC TTGGCTATGA AAGTGGCTTC ACCACCTCCA
    ACCACTCAAC CTACAAAGAA AGCTGGAGAT GTCACTAATG AGTTGGTGAG GCATTTTCTA
    ATTGAGACGA GTCCGAAGGG TGTGAGACTG AAGGGCTGCC CCAACGAGCC GTACTTTGGC
    TGCCTGTCTG CGCTGGTCTA CCAGCACTCC ATCACTCCAC TGGCACTGCC CTGCAAACTA
    GTCATACCAA CCAGAGATCC TCTGGAAGAG TCACCTGAGA TCGCAACACC AACCAACCCG
    GCGGCAGATA TGCTCAAGCA GGGCGCAGGG CAGAAGAGTT CAGCTGATGG CCATGCATGC
    AATGTGCTGT ACATCAACTC TGTGGATATG GAGTCTTTGA CTGGACCTCA AGCCATCGCC
    AAAGCCATTT CAGAAACCAT GAACGCCAGT CCGGCACCCT CAGCCACCAT TGTTCACTTC
    AAGGTGTCGG CACAAGGCAT CACACTCACA GACAACCAGA GAAAGCTTTT CTTCAGACGT
    CATTATCCCA TCGGCACAGT CACGTTCTGT GACATTGATC CACAGGAGAG GAAGTGGAAC
    AAACCAGAGG GTGGCACTGC CAAATTCTTT GGCTTTGTGG CTAGAAAGCA AGGCAGCACG
    ACAGACAATG TCAGTCACCT GTTTGCTGAG ATGGACCCAG ATCAGCCGGC CACCGCCATC
    GTCAACTTTG TCTCTAAAGT GATGATCAGC TCTCAGAAGC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144122
    Clone ID Related Accession (Same CDS sequence) XM_021479036.1
    Accession Version XM_021479036.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product tensin-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCACCG CTGTGTTTCA GGTCTCGTCC TCCTGTGTTC CTGTCGCCAA CTATGAACTG 
    GCCCCCAGCA ATGAGCTCCC CCTGAAACAT ATCGAGACCA CAGGCTCAAC CAAATCCTCC
    AAGAGCATGG AGTCTCGACG CAGACCGTCA AGGAGTTTGA GTTTGATCCA GGCCATGGAG
    GAGAACTATG AGGTGGATCT GGTTTACATC ACCGAGCGCA TCATTTCTGT CACCTTTCCC
    AGTAATGCAG AGGAGCCCAG TTACAGCGCC AACATTAAAG AAGTGGCAAA CATGCTGCGC
    TCCAAACATG GAGACAACTA CCTGCTCTTC AACCTCAGTG AAAAGCGCTT GGATATCTCC
    AAGCTGAATC CCAAAGTTCT GGATTTTGGC TGGCCGGATC ATCACGCTCC TGCTTTGGAT
    AAGATCTGCA GCATCTGCAA AGCCATGGAC ACTTGGATGA ACGCTGGCAG TCACAACGTG
    GTGGTCATAC ACAACAAGGG AAACCGAGGT CGAACTGGGG TGGTTGTGGC TGCGTACATG
    CATTACAGCA ACATATCAGC GAGTGCCGAC CAGGCTCTGG ACAGGTTTGC CATGAAGAGG
    TTTTATGAAG ATAAAGTGCT TCCTGTGGGT CAGCCTTCCC AGAGAAGATA TGTGCAGTAC
    TTCAGCGGTC TGCTCTCCGG CCACATTAAG ATCAACAACA AACCCCTGTT TCTCCATCAC
    GTCATCATGC ACGGCATCCC AAACTTCGAG TCTAAAGGAG GTTGCCGCCC TTTCCTGAAG
    ATCTACCAGG CCATGCAGCC CGTCTACACA TCTGGCATCT ATAATGTCCA AGGAGACAGT
    CAGACTAGTA TCTGCATCAC CATTGAACCG GGCCTGCTTT TGAAAGGAGA CATACTGCTA
    AAGTGTTATC ACAAGCGTTT CCGCAGCCCA TGCCGTGATG TCATATTCAG AGTTCAGTTT
    CACACCTGTG CTGTTCATGA TCTGGGTATC GTCTTCGGCA AAGATGAGCT TGATGAAACC
    TTTAAAGACG ACAGATTTCC AGAATATGGA AAGGTGGAAT TCGTCTTTTC TTTTGGCCCA
    GAAAAAATCC AAGGAATGGA CCACCTTGAG AACGGGCCCA GTGTTTCTGT GGACTACAAT
    ACCCAAGATG CACTAATCCG CTGGGATTCC TACGAGAACT TCAACCAGCG CTGTGAAGAC
    TCCATGGAAG ACGTTATTCA CACCGAAGGT CCATTGGATG GTAGTCTCTA CGCTAAAGTT
    CGTAAGAAAG AGTCTCTGGA AGGAGGGGTT ACAGCCAATG GTCTCCCAGC TGCTGCTGAT
    CACACTTTAC CTGCTGTAGA CCACACTCTA CCACCAGTTG ACCACGCCTT GTCTGTAAGC
    AGTGATTCAG GCAACTCCAC AGCGTCCATC AAGACTGACC GCACTGATGA AGCCAGCCAT
    CAGCCAGCAT TGGTGAGTCA GCCATCTTCC CAGCCCCTCA GCCCTGAGGA GAAACAGGAG
    CTGGACCAGT TGCTCAGTGG TTTGGAAGCA CCAATGCATC GCACAGGTTA CCAAAGCAGC
    CCTGGTGGGG GTGGTGGTGG AGGTGGTGGA GTCCTACATC TCGTCCCTGC CCAGGTACAC
    GTCAATGGAC ACAGCAACAT AGACAGAGAG ACAGATATCC TTGATGATGA CTTGCCCATC
    AGCCAGGAAG GGAACAGTGT GGACAGTCTT GGAACTCTCT CATCTTTTGA GGGCAGAGCC
    ACTCCTGCGG ATCTATACTA CCAGTCAGAG ACAGTCATTA ATGGACAAGA TATGCCCTAC
    TTAGAGAAGA GTGTCCATGA GAAAATGCTA GAGCCCAGAG TTCACAGCTC ATCATCTGTC
    CAAGAGCACT TGGGAGCCCC TAGTGGGTAT GCTAACCAGA ATGGCAACAT CTATCGATCT
    CAGTCATTAG GAGCAACCCC GAACACAGAC CAGAAGTACA TGCCCAAAGC TCCAGCACGC
    ACAACCAGCA GTAGGGATGC CGTGCAGAGA CTGAATGTTT GGCAACAATT TGGTGTACCA
    GAAGTCCCAG TCACAGATGG GGTCACCTTC AACCCTGTAC TACAATCCCA CAGCCTTCCA
    GAGTTCCCTC AGATGGCATC TCAACAAGAG ATTGAGCAGT CCATTGAAGC CCTCAACATG
    CTGATGCTGG ACCTTGATCC AGCACTTACT CAAGTCCCCA AATCCCACAG CGCTCCATCT
    GGAGAAAGTG GTGTGGTCAC CACCCAACCA TCTTTCTCTC AAACCCAAGC ACGCCCATCC
    TACCAGGCTG ACACTGCTAT TCATGACCGC GGTGCGGCCT CTTCAATGGG ATTTCCTTTG
    ACCTTTCAAC AAGTAACTGG AGAGCCTTCA CAACCCACCA TGAAACAGCA GAAACCCACA
    ACTACATCCC AGCATGTCGA ATTCGCTTCT GAAGCAGGAG GTCAGGCCCC CATGTCCCAG
    TCCACAGTGC CTTCCTCCAC CTTTGGAGTT CTCCAGTTGA AACCCCTCAA CATTTATCCC
    TCAAGTACCA CATCCCAGTC CCCCGAGCCT CAGGAGACTC AGCGTAGCTA CTCTTCTTCC
    TCATCCCCTC TTCCCAAAGA GTCTGAACCA GAAGATAGCA GCTACAAATT GGAGGGTCTT
    GTAGCTCACC GTGTAGCTGA GTACACTGCT CGTATCCGCT GCATCAATGA CAGCCAGAGT
    GCTCCCGAGT TTGGCCATCG CCGCCGCTCT CTCTCCTTCA GTGGACTTCC ATCTGGTGAA
    ATGTCGCCAG ATGAGGCTGC AGTTCCTGGA CGCTGTCGGA CCCTTAGTGA GGGCCTTTCC
    CAAGACGAAA GCCCAACTCG AGGAAGCTCA GTCCGCTCCC CCATTCGCTG CATCTCACCA
    GAGCTGGCCA ATACAATTGC TCTGAACCCT GGGGGGCGGC CCAAGGAGAG GCACATGCAC
    AGCTACAGAG AAGCGTTTGA GGAAATGGAT GGGGGTCCCG TTAGCCCCCC TCCAATTGTT
    GGTGGTGAGG TCCTACCCCA AACCCCTGCC TTCCCTGTCT CGCCACAAAC CCCTTACTTC
    AACATGTGTC GATCTCCTCC AGGATTGGCT AAAACACCAC TGTCCTTTCT GGGCCTGAAG
    CCCCACAACC CTGCTGAGAT CCTGCTCAAC CAGACAGGCT CAGAGAGTGA GGATGAGGAA
    GAGCCTCGTA GTTATGTGGA GTCTGTGGCG AAGGCAGCAG CAGTACCTGC ATCTCCAACA
    TATGACAGCG ACAGCTTAGC TCGTCCTGCC GGACCGTCCC ATGCTTACAA TGCTCCACTT
    TCCTCCAGCA GCCCCATTCA GAACCCAGAT CCAGTTGAAA GCACATTGGC TGACAGCGGG
    GTCGGGATGA ATTCTTTATC CCAGGCTGCT GTCGACATTT TCCATTTCCA GCCCTCCGAA
    ATCCCATACC AACCACCACC ACCCCCCTAC CCAACCTCCA TGTTGGGTAG CTACATCAGC
    CCTTCTCCAG CAGAGGCCAT GCAAAATAAT ACCACCACCA TCCAGCCACT TTCTGGAAGT
    CCTGGGACAA GACACCACAC CATCCTTTCT GACATGGCGT CCGTTCCCAA CTTTCAACAC
    AGATTAGCAA ATCAGGATGG TCCCATACTT GGGCGACAGC AGGCTCCAGC TAATGGTCAT
    GTGCCTGGAA TTGCTGGTAG TCCAGTGCAA GGAAGACACA CTATGGTGTC CCAGGGTACA
    CAAAGCAGTC CCATACTGAG CAGACAGTAT CCTGTCACCC AGGGGATTCA GAGTAGTCCA
    ATTCTCAGCA GGCAGCCAAT GGGACAAGCT GTCCAGAGCA GTCCAGTCCT CAACCGACAG
    GCATCCCTGA ATCAAGCAAA CCAAAGCAGT CCAGTGTTGA GTAGACAGCA GTCCATTACC
    CTACCCAACC AAGGCAGCCC GGTCCTAAGC AGACAGTCCT CGCTTAGTCA TCCCAGTCAG
    GGTAGTCCTG TACTAGGACG CCACCCTTCC CTAACACAGG GTAGCCCCAG CCTGGATAGG
    CATCCGATGT ACAGCGGTTA CACCACTCCA GAAGAGCGAC ACGGAGCTCT GTCACGCCAA
    AGCAGCTCTT CAGGATACCA GCCGCCATCT ACACCGTCTT TCCCAGTTTC TCCAGCAGGA
    TACATCGAGG GAGCGGGATT CAGACAGGGG AGTCCTGCTT TCCAACCTCA GTTGCCCGAA
    AAGAGACGCA TGTCCAGCAG TGACCGCACC AATGGTGGCC TGTCCTATGG GACGCTCAAT
    GGGAAGATGT CCTCACCGGT GTCCAGTGGC GGCAGCACTC CGAGTGTTCA TTTCTTCCAC
    ACACTCCCCG ATTTCTCCAA ACTCAACATG TGTGATGGAA GTCCAGAAAC CAGAATGAAC
    GTGAAGTTTG TCCAGGACAC GTCCAAATTC TGGTATAAAC CAGATATCTC GAGAGACCAA
    GCGATTAACA TGCTGAAGGA TCAAGATCCT GGGGCCTTTG TGATTCGGGA CAGTCATTCA
    TTCAGAGGAG CCTACGGCTT GGCTATGAAA GTGGCTTCAC CACCTCCAAC CACTCAACCT
    ACAAAGAAAG CTGGAGATGT CACTAATGAG TTGGTGAGGC ATTTTCTAAT TGAGACGAGT
    CCGAAGGGTG TGAGACTGAA GGGCTGCCCC AACGAGCCGT ACTTTGGCTG CCTGTCTGCG
    CTGGTCTACC AGCACTCCAT CACTCCACTG GCACTGCCCT GCAAACTAGT CATACCAACC
    AGAGATCCTC TGGAAGAGTC ACCTGAGATC GCAACACCAA CCAACCCGGC GGCAGATATG
    CTCAAGCAGG GCGCAGGGCA GAAGAGTTCA GCTGATGGCC ATGCATGCAA TGTGCTGTAC
    ATCAACTCTG TGGATATGGA GTCTTTGACT GGACCTCAAG CCATCGCCAA AGCCATTTCA
    GAAACCATGA ACGCCAGTCC GGCACCCTCA GCCACCATTG TTCACTTCAA GGTGTCGGCA
    CAAGGCATCA CACTCACAGA CAACCAGAGA AAGCTTTTCT TCAGACGTCA TTATCCCATC
    GGCACAGTCA CGTTCTGTGA CATTGATCCA CAGGAGAGGA AGTGGAACAA ACCAGAGGGT
    GGCACTGCCA AATTCTTTGG CTTTGTGGCT AGAAAGCAAG GCAGCACGAC AGACAATGTC
    AGTCACCTGT TTGCTGAGAT GGACCCAGAT CAGCCGGCCA CCGCCATCGT CAACTTTGTC
    TCTAAAGTGA TGATCAGCTC TCAGAAGCGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334711.1
    CDS414..5666
    Translation

    Target ORF information:

    RefSeq Version XM_021479036.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479036.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCACCG CTGTGTTTCA GGTCTCGTCC TCCTGTGTTC CTGTCGCCAA CTATGAACTG 
    GCCCCCAGCA ATGAGCTCCC CCTGAAACAT ATCGAGACCA CAGGCTCAAC CAAATCCTCC
    AAGAGCATGG AGTCTCGACG CAGACCGTCA AGGAGTTTGA GTTTGATCCA GGCCATGGAG
    GAGAACTATG AGGTGGATCT GGTTTACATC ACCGAGCGCA TCATTTCTGT CACCTTTCCC
    AGTAATGCAG AGGAGCCCAG TTACAGCGCC AACATTAAAG AAGTGGCAAA CATGCTGCGC
    TCCAAACATG GAGACAACTA CCTGCTCTTC AACCTCAGTG AAAAGCGCTT GGATATCTCC
    AAGCTGAATC CCAAAGTTCT GGATTTTGGC TGGCCGGATC ATCACGCTCC TGCTTTGGAT
    AAGATCTGCA GCATCTGCAA AGCCATGGAC ACTTGGATGA ACGCTGGCAG TCACAACGTG
    GTGGTCATAC ACAACAAGGG AAACCGAGGT CGAACTGGGG TGGTTGTGGC TGCGTACATG
    CATTACAGCA ACATATCAGC GAGTGCCGAC CAGGCTCTGG ACAGGTTTGC CATGAAGAGG
    TTTTATGAAG ATAAAGTGCT TCCTGTGGGT CAGCCTTCCC AGAGAAGATA TGTGCAGTAC
    TTCAGCGGTC TGCTCTCCGG CCACATTAAG ATCAACAACA AACCCCTGTT TCTCCATCAC
    GTCATCATGC ACGGCATCCC AAACTTCGAG TCTAAAGGAG GTTGCCGCCC TTTCCTGAAG
    ATCTACCAGG CCATGCAGCC CGTCTACACA TCTGGCATCT ATAATGTCCA AGGAGACAGT
    CAGACTAGTA TCTGCATCAC CATTGAACCG GGCCTGCTTT TGAAAGGAGA CATACTGCTA
    AAGTGTTATC ACAAGCGTTT CCGCAGCCCA TGCCGTGATG TCATATTCAG AGTTCAGTTT
    CACACCTGTG CTGTTCATGA TCTGGGTATC GTCTTCGGCA AAGATGAGCT TGATGAAACC
    TTTAAAGACG ACAGATTTCC AGAATATGGA AAGGTGGAAT TCGTCTTTTC TTTTGGCCCA
    GAAAAAATCC AAGGAATGGA CCACCTTGAG AACGGGCCCA GTGTTTCTGT GGACTACAAT
    ACCCAAGATG CACTAATCCG CTGGGATTCC TACGAGAACT TCAACCAGCG CTGTGAAGAC
    TCCATGGAAG ACGTTATTCA CACCGAAGGT CCATTGGATG GTAGTCTCTA CGCTAAAGTT
    CGTAAGAAAG AGTCTCTGGA AGGAGGGGTT ACAGCCAATG GTCTCCCAGC TGCTGCTGAT
    CACACTTTAC CTGCTGTAGA CCACACTCTA CCACCAGTTG ACCACGCCTT GTCTGTAAGC
    AGTGATTCAG GCAACTCCAC AGCGTCCATC AAGACTGACC GCACTGATGA AGCCAGCCAT
    CAGCCAGCAT TGGTGAGTCA GCCATCTTCC CAGCCCCTCA GCCCTGAGGA GAAACAGGAG
    CTGGACCAGT TGCTCAGTGG TTTGGAAGCA CCAATGCATC GCACAGGTTA CCAAAGCAGC
    CCTGGTGGGG GTGGTGGTGG AGGTGGTGGA GTCCTACATC TCGTCCCTGC CCAGGTACAC
    GTCAATGGAC ACAGCAACAT AGACAGAGAG ACAGATATCC TTGATGATGA CTTGCCCATC
    AGCCAGGAAG GGAACAGTGT GGACAGTCTT GGAACTCTCT CATCTTTTGA GGGCAGAGCC
    ACTCCTGCGG ATCTATACTA CCAGTCAGAG ACAGTCATTA ATGGACAAGA TATGCCCTAC
    TTAGAGAAGA GTGTCCATGA GAAAATGCTA GAGCCCAGAG TTCACAGCTC ATCATCTGTC
    CAAGAGCACT TGGGAGCCCC TAGTGGGTAT GCTAACCAGA ATGGCAACAT CTATCGATCT
    CAGTCATTAG GAGCAACCCC GAACACAGAC CAGAAGTACA TGCCCAAAGC TCCAGCACGC
    ACAACCAGCA GTAGGGATGC CGTGCAGAGA CTGAATGTTT GGCAACAATT TGGTGTACCA
    GAAGTCCCAG TCACAGATGG GGTCACCTTC AACCCTGTAC TACAATCCCA CAGCCTTCCA
    GAGTTCCCTC AGATGGCATC TCAACAAGAG ATTGAGCAGT CCATTGAAGC CCTCAACATG
    CTGATGCTGG ACCTTGATCC AGCACTTACT CAAGTCCCCA AATCCCACAG CGCTCCATCT
    GGAGAAAGTG GTGTGGTCAC CACCCAACCA TCTTTCTCTC AAACCCAAGC ACGCCCATCC
    TACCAGGCTG ACACTGCTAT TCATGACCGC GGTGCGGCCT CTTCAATGGG ATTTCCTTTG
    ACCTTTCAAC AAGTAACTGG AGAGCCTTCA CAACCCACCA TGAAACAGCA GAAACCCACA
    ACTACATCCC AGCATGTCGA ATTCGCTTCT GAAGCAGGAG GTCAGGCCCC CATGTCCCAG
    TCCACAGTGC CTTCCTCCAC CTTTGGAGTT CTCCAGTTGA AACCCCTCAA CATTTATCCC
    TCAAGTACCA CATCCCAGTC CCCCGAGCCT CAGGAGACTC AGCGTAGCTA CTCTTCTTCC
    TCATCCCCTC TTCCCAAAGA GTCTGAACCA GAAGATAGCA GCTACAAATT GGAGGGTCTT
    GTAGCTCACC GTGTAGCTGA GTACACTGCT CGTATCCGCT GCATCAATGA CAGCCAGAGT
    GCTCCCGAGT TTGGCCATCG CCGCCGCTCT CTCTCCTTCA GTGGACTTCC ATCTGGTGAA
    ATGTCGCCAG ATGAGGCTGC AGTTCCTGGA CGCTGTCGGA CCCTTAGTGA GGGCCTTTCC
    CAAGACGAAA GCCCAACTCG AGGAAGCTCA GTCCGCTCCC CCATTCGCTG CATCTCACCA
    GAGCTGGCCA ATACAATTGC TCTGAACCCT GGGGGGCGGC CCAAGGAGAG GCACATGCAC
    AGCTACAGAG AAGCGTTTGA GGAAATGGAT GGGGGTCCCG TTAGCCCCCC TCCAATTGTT
    GGTGGTGAGG TCCTACCCCA AACCCCTGCC TTCCCTGTCT CGCCACAAAC CCCTTACTTC
    AACATGTGTC GATCTCCTCC AGGATTGGCT AAAACACCAC TGTCCTTTCT GGGCCTGAAG
    CCCCACAACC CTGCTGAGAT CCTGCTCAAC CAGACAGGCT CAGAGAGTGA GGATGAGGAA
    GAGCCTCGTA GTTATGTGGA GTCTGTGGCG AAGGCAGCAG CAGTACCTGC ATCTCCAACA
    TATGACAGCG ACAGCTTAGC TCGTCCTGCC GGACCGTCCC ATGCTTACAA TGCTCCACTT
    TCCTCCAGCA GCCCCATTCA GAACCCAGAT CCAGTTGAAA GCACATTGGC TGACAGCGGG
    GTCGGGATGA ATTCTTTATC CCAGGCTGCT GTCGACATTT TCCATTTCCA GCCCTCCGAA
    ATCCCATACC AACCACCACC ACCCCCCTAC CCAACCTCCA TGTTGGGTAG CTACATCAGC
    CCTTCTCCAG CAGAGGCCAT GCAAAATAAT ACCACCACCA TCCAGCCACT TTCTGGAAGT
    CCTGGGACAA GACACCACAC CATCCTTTCT GACATGGCGT CCGTTCCCAA CTTTCAACAC
    AGATTAGCAA ATCAGGATGG TCCCATACTT GGGCGACAGC AGGCTCCAGC TAATGGTCAT
    GTGCCTGGAA TTGCTGGTAG TCCAGTGCAA GGAAGACACA CTATGGTGTC CCAGGGTACA
    CAAAGCAGTC CCATACTGAG CAGACAGTAT CCTGTCACCC AGGGGATTCA GAGTAGTCCA
    ATTCTCAGCA GGCAGCCAAT GGGACAAGCT GTCCAGAGCA GTCCAGTCCT CAACCGACAG
    GCATCCCTGA ATCAAGCAAA CCAAAGCAGT CCAGTGTTGA GTAGACAGCA GTCCATTACC
    CTACCCAACC AAGGCAGCCC GGTCCTAAGC AGACAGTCCT CGCTTAGTCA TCCCAGTCAG
    GGTAGTCCTG TACTAGGACG CCACCCTTCC CTAACACAGG GTAGCCCCAG CCTGGATAGG
    CATCCGATGT ACAGCGGTTA CACCACTCCA GAAGAGCGAC ACGGAGCTCT GTCACGCCAA
    AGCAGCTCTT CAGGATACCA GCCGCCATCT ACACCGTCTT TCCCAGTTTC TCCAGCAGGA
    TACATCGAGG GAGCGGGATT CAGACAGGGG AGTCCTGCTT TCCAACCTCA GTTGCCCGAA
    AAGAGACGCA TGTCCAGCAG TGACCGCACC AATGGTGGCC TGTCCTATGG GACGCTCAAT
    GGGAAGATGT CCTCACCGGT GTCCAGTGGC GGCAGCACTC CGAGTGTTCA TTTCTTCCAC
    ACACTCCCCG ATTTCTCCAA ACTCAACATG TGTGATGGAA GTCCAGAAAC CAGAATGAAC
    GTGAAGTTTG TCCAGGACAC GTCCAAATTC TGGTATAAAC CAGATATCTC GAGAGACCAA
    GCGATTAACA TGCTGAAGGA TCAAGATCCT GGGGCCTTTG TGATTCGGGA CAGTCATTCA
    TTCAGAGGAG CCTACGGCTT GGCTATGAAA GTGGCTTCAC CACCTCCAAC CACTCAACCT
    ACAAAGAAAG CTGGAGATGT CACTAATGAG TTGGTGAGGC ATTTTCTAAT TGAGACGAGT
    CCGAAGGGTG TGAGACTGAA GGGCTGCCCC AACGAGCCGT ACTTTGGCTG CCTGTCTGCG
    CTGGTCTACC AGCACTCCAT CACTCCACTG GCACTGCCCT GCAAACTAGT CATACCAACC
    AGAGATCCTC TGGAAGAGTC ACCTGAGATC GCAACACCAA CCAACCCGGC GGCAGATATG
    CTCAAGCAGG GCGCAGGGCA GAAGAGTTCA GCTGATGGCC ATGCATGCAA TGTGCTGTAC
    ATCAACTCTG TGGATATGGA GTCTTTGACT GGACCTCAAG CCATCGCCAA AGCCATTTCA
    GAAACCATGA ACGCCAGTCC GGCACCCTCA GCCACCATTG TTCACTTCAA GGTGTCGGCA
    CAAGGCATCA CACTCACAGA CAACCAGAGA AAGCTTTTCT TCAGACGTCA TTATCCCATC
    GGCACAGTCA CGTTCTGTGA CATTGATCCA CAGGAGAGGA AGTGGAACAA ACCAGAGGGT
    GGCACTGCCA AATTCTTTGG CTTTGTGGCT AGAAAGCAAG GCAGCACGAC AGACAATGTC
    AGTCACCTGT TTGCTGAGAT GGACCCAGAT CAGCCGGCCA CCGCCATCGT CAACTTTGTC
    TCTAAAGTGA TGATCAGCTC TCAGAAGCGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144123
    Clone ID Related Accession (Same CDS sequence) XM_021479037.1
    Accession Version XM_021479037.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5445bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product tensin-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
    CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA
    GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT
    TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT
    CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC
    CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG
    AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG
    AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT
    AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC
    AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG
    CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG
    ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG
    GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT
    TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT
    TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC
    AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC
    ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC
    TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG
    ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG
    TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC
    ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT
    AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA
    AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC
    CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC
    ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT
    AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC
    ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT
    GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG
    CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG
    GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT
    GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC
    AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC
    CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT
    CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA
    GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA
    GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG
    TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA
    ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA
    GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG
    TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG
    ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA
    GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC
    CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC
    TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT
    ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC
    ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA
    AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA
    TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA
    GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT
    CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG
    TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA
    GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG
    CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC
    TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT
    GGTGAGGTCC TACCCCAAAC CCCTGCCTTC CCTGTCTCGC CACAAACCCC TTACTTCAAC
    ATGTGTCGAT CTCCTCCAGG ATTGGCTAAA ACACCACTGT CCTTTCTGGG CCTGAAGCCC
    CACAACCCTG CTGAGATCCT GCTCAACCAG ACAGGCTCAG AGCCTCGTAG TTATGTGGAG
    TCTGTGGCGA AGGCAGCAGC AGTACCTGCA TCTCCAACAT ATGACAGCGA CAGCTTAGCT
    CGTCCTGCCG GACCGTCCCA TGCTTACAAT GCTCCACTTT CCTCCAGCAG CCCCATTCAG
    AACCCAGATC CAGTTGAAAG CACATTGGCT GACAGCGGGG TCGGGATGAA TTCTTTATCC
    CAGGCTGCTG TCGACATTTT CCATTTCCAG CCCTCCGAAA TCCCATACCA ACCACCACCA
    CCCCCCTACC CAACCTCCAT GTTGGGTAGC TACATCAGCC CTTCTCCAGC AGAGGCCATG
    CAAAATAATA CCACCACCAT CCAGCCACTT TCTGGAAGTC CTGGGACAAG ACACCACACC
    ATCCTTTCTG ACATGGCGTC CGTTCCCAAC TTTCAACACA GATTAGCAAA TCAGGATGGT
    CCCATACTTG GGCGACAGCA GGCTCCAGCT AATGGTCATG TGCCTGGAAT TGCTGGTAGT
    CCAGTGCAAG GAAGACACAC TATGGTGTCC CAGGGTACAC AAAGCAGTCC CATACTGAGC
    AGACAGTATC CTGTCACCCA GGGGATTCAG AGTAGTCCAA TTCTCAGCAG GCAGCCAATG
    GGACAAGCTG TCCAGAGCAG TCCAGTCCTC AACCGACAGG CATCCCTGAA TCAAGCAAAC
    CAAAGCAGTC CAGTGTTGAG TAGACAGCAG TCCATTACCC TACCCAACCA AGGCAGCCCG
    GTCCTAAGCA GACAGTCCTC GCTTAGTCAT CCCAGTCAGG GTAGTCCTGT ACTAGGACGC
    CACCCTTCCC TAACACAGGG TAGCCCCAGC CTGGATAGGC ATCCGATGTA CAGCGGTTAC
    ACCACTCCAG AAGAGCGACA CGGAGCTCTG TCACGCCAAA GCAGCTCTTC AGGATACCAG
    CCGCCATCTA CACCGTCTTT CCCAGTTTCT CCAGCAGGAT ACATCGAGGG AGCGGGATTC
    AGACAGGGGA GTCCTGCTTT CCAACCTCAG TTGCCCGAAA AGAGACGCAT GTCCAGCAGT
    GACCGCACCA ATGGTGGCCT GTCCTATGGG ACGCTCAATG GGAAGATGTC CTCACCGGTG
    TCCAGTGGCG GCAGCACTCC GAGTGTTCAT TTCTTCCACA CACTCCCCGA TTTCTCCAAA
    CTCAACATGT GTGATGGAAG TCCAGAAACC AGAATGAACG TGAAGTTTGT CCAGGACACG
    TCCAAATTCT GGTATAAACC AGATATCTCG AGAGACCAAG CGATTAACAT GCTGAAGGAT
    CAAGATCCTG GGGCCTTTGT GATTCGGGAC AGTCATTCAT TCAGAGGAGC CTACGGCTTG
    GCTATGAAAG TGGCTTCACC ACCTCCAACC ACTCAACCTA CAAAGAAAGC TGGAGATGTC
    ACTAATGAGT TGGTGAGGCA TTTTCTAATT GAGACGAGTC CGAAGGGTGT GAGACTGAAG
    GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC CTGTCTGCGC TGGTCTACCA GCACTCCATC
    ACTCCACTGG CACTGCCCTG CAAACTAGTC ATACCAACCA GAGATCCTCT GGAAGAGTCA
    CCTGAGATCG CAACACCAAC CAACCCGGCG GCAGATATGC TCAAGCAGGG CGCAGCATGC
    AATGTGCTGT ACATCAACTC TGTGGATATG GAGTCTTTGA CTGGACCTCA AGCCATCGCC
    AAAGCCATTT CAGAAACCAT GAACGCCAGT CCGGCACCCT CAGCCACCAT TGTTCACTTC
    AAGGTGTCGG CACAAGGCAT CACACTCACA GACAACCAGA GAAAGCTTTT CTTCAGACGT
    CATTATCCCA TCGGCACAGT CACGTTCTGT GACATTGATC CACAGGAGAG GAAGTGGAAC
    AAACCAGAGG GTGGCACTGC CAAATTCTTT GGCTTTGTGG CTAGAAAGCA AGGCAGCACG
    ACAGACAATG TCAGTCACCT GTTTGCTGAG ATGGACCCAG ATCAGCCGGC CACCGCCATC
    GTCAACTTTG TCTCTAAAGT GATGATCAGC TCTCAGAAGC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334712.1
    CDS284..5728
    Translation

    Target ORF information:

    RefSeq Version XM_021479037.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479037.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
    CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA
    GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT
    TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT
    CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC
    CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG
    AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG
    AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT
    AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC
    AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG
    CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG
    ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG
    GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT
    TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT
    TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC
    AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC
    ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC
    TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG
    ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG
    TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC
    ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT
    AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA
    AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC
    CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC
    ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT
    AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC
    ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT
    GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG
    CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG
    GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT
    GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC
    AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC
    CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT
    CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA
    GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA
    GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG
    TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA
    ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA
    GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG
    TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG
    ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA
    GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC
    CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC
    TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT
    ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC
    ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA
    AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA
    TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA
    GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT
    CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG
    TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA
    GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG
    CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC
    TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT
    GGTGAGGTCC TACCCCAAAC CCCTGCCTTC CCTGTCTCGC CACAAACCCC TTACTTCAAC
    ATGTGTCGAT CTCCTCCAGG ATTGGCTAAA ACACCACTGT CCTTTCTGGG CCTGAAGCCC
    CACAACCCTG CTGAGATCCT GCTCAACCAG ACAGGCTCAG AGCCTCGTAG TTATGTGGAG
    TCTGTGGCGA AGGCAGCAGC AGTACCTGCA TCTCCAACAT ATGACAGCGA CAGCTTAGCT
    CGTCCTGCCG GACCGTCCCA TGCTTACAAT GCTCCACTTT CCTCCAGCAG CCCCATTCAG
    AACCCAGATC CAGTTGAAAG CACATTGGCT GACAGCGGGG TCGGGATGAA TTCTTTATCC
    CAGGCTGCTG TCGACATTTT CCATTTCCAG CCCTCCGAAA TCCCATACCA ACCACCACCA
    CCCCCCTACC CAACCTCCAT GTTGGGTAGC TACATCAGCC CTTCTCCAGC AGAGGCCATG
    CAAAATAATA CCACCACCAT CCAGCCACTT TCTGGAAGTC CTGGGACAAG ACACCACACC
    ATCCTTTCTG ACATGGCGTC CGTTCCCAAC TTTCAACACA GATTAGCAAA TCAGGATGGT
    CCCATACTTG GGCGACAGCA GGCTCCAGCT AATGGTCATG TGCCTGGAAT TGCTGGTAGT
    CCAGTGCAAG GAAGACACAC TATGGTGTCC CAGGGTACAC AAAGCAGTCC CATACTGAGC
    AGACAGTATC CTGTCACCCA GGGGATTCAG AGTAGTCCAA TTCTCAGCAG GCAGCCAATG
    GGACAAGCTG TCCAGAGCAG TCCAGTCCTC AACCGACAGG CATCCCTGAA TCAAGCAAAC
    CAAAGCAGTC CAGTGTTGAG TAGACAGCAG TCCATTACCC TACCCAACCA AGGCAGCCCG
    GTCCTAAGCA GACAGTCCTC GCTTAGTCAT CCCAGTCAGG GTAGTCCTGT ACTAGGACGC
    CACCCTTCCC TAACACAGGG TAGCCCCAGC CTGGATAGGC ATCCGATGTA CAGCGGTTAC
    ACCACTCCAG AAGAGCGACA CGGAGCTCTG TCACGCCAAA GCAGCTCTTC AGGATACCAG
    CCGCCATCTA CACCGTCTTT CCCAGTTTCT CCAGCAGGAT ACATCGAGGG AGCGGGATTC
    AGACAGGGGA GTCCTGCTTT CCAACCTCAG TTGCCCGAAA AGAGACGCAT GTCCAGCAGT
    GACCGCACCA ATGGTGGCCT GTCCTATGGG ACGCTCAATG GGAAGATGTC CTCACCGGTG
    TCCAGTGGCG GCAGCACTCC GAGTGTTCAT TTCTTCCACA CACTCCCCGA TTTCTCCAAA
    CTCAACATGT GTGATGGAAG TCCAGAAACC AGAATGAACG TGAAGTTTGT CCAGGACACG
    TCCAAATTCT GGTATAAACC AGATATCTCG AGAGACCAAG CGATTAACAT GCTGAAGGAT
    CAAGATCCTG GGGCCTTTGT GATTCGGGAC AGTCATTCAT TCAGAGGAGC CTACGGCTTG
    GCTATGAAAG TGGCTTCACC ACCTCCAACC ACTCAACCTA CAAAGAAAGC TGGAGATGTC
    ACTAATGAGT TGGTGAGGCA TTTTCTAATT GAGACGAGTC CGAAGGGTGT GAGACTGAAG
    GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC CTGTCTGCGC TGGTCTACCA GCACTCCATC
    ACTCCACTGG CACTGCCCTG CAAACTAGTC ATACCAACCA GAGATCCTCT GGAAGAGTCA
    CCTGAGATCG CAACACCAAC CAACCCGGCG GCAGATATGC TCAAGCAGGG CGCAGCATGC
    AATGTGCTGT ACATCAACTC TGTGGATATG GAGTCTTTGA CTGGACCTCA AGCCATCGCC
    AAAGCCATTT CAGAAACCAT GAACGCCAGT CCGGCACCCT CAGCCACCAT TGTTCACTTC
    AAGGTGTCGG CACAAGGCAT CACACTCACA GACAACCAGA GAAAGCTTTT CTTCAGACGT
    CATTATCCCA TCGGCACAGT CACGTTCTGT GACATTGATC CACAGGAGAG GAAGTGGAAC
    AAACCAGAGG GTGGCACTGC CAAATTCTTT GGCTTTGTGG CTAGAAAGCA AGGCAGCACG
    ACAGACAATG TCAGTCACCT GTTTGCTGAG ATGGACCCAG ATCAGCCGGC CACCGCCATC
    GTCAACTTTG TCTCTAAAGT GATGATCAGC TCTCAGAAGC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60375
    Clone ID Related Accession (Same CDS sequence) XM_017357846.1
    Accession Version XM_017357846.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product tensin-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
    TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG
    CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG
    GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG
    TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA
    CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG
    TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC
    ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC
    GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC
    TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT
    GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG
    GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA
    GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC
    GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG
    GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT
    AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC
    GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC
    ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA
    CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC
    CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT
    ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT
    GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT
    GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT
    TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA
    GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG
    GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT
    CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC
    ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT
    TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA
    GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT
    GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA
    GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT
    CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA
    GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG
    CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG
    TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA
    GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG
    AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC
    TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA
    GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT
    ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA
    CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC
    CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT
    TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT
    TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA
    GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG
    CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA
    CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA
    TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG
    GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC
    ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG
    CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT
    CCAATTGTTG GTGGTGAGGT CCTACCCCAA ACCCCTGCCT TCCCTGTCTC GCCACAAACC
    CCTTACTTCA ACATGTGTCG ATCTCCTCCA GGATTGGCTA AAACACCACT GTCCTTTCTG
    GGCCTGAAGC CCCACAACCC TGCTGAGATC CTGCTCAACC AGACAGGCTC AGAGAGTGAG
    GATGAGGAAG AGCCTCGTAG TTATGTGGAG TCTGTGGCGA AGGCAGCAGC AGTACCTGCA
    TCTCCAACAT ATGACAGCGA CAGCTTAGCT CGTCCTGCCG GACCGTCCCA TGCTTACAAT
    GCTCCACTTT CCTCCAGCAG CCCCATTCAG AACCCAGATC CAGTTGAAAG CACATTGGCT
    GACAGCGGGG TCGGGATGAA TTCTTTATCC CAGGCTGCTG TCGACATTTT CCATTTCCAG
    CCCTCCGAAA TCCCATACCA ACCACCACCA CCCCCCTACC CAACCTCCAT GTTGGGTAGC
    TACATCAGCC CTTCTCCAGC AGAGGCCATG CAAAATAATA CCACCACCAT CCAGCCACTT
    TCTGGAAGTC CTGGGACAAG ACACCACACC ATCCTTTCTG ACATGGCGTC CGTTCCCAAC
    TTTCAACACA GATTAGCAAA TCAGGATGGT CCCATACTTG GGCGACAGCA GGCTCCAGCT
    AATGGTCATG TGCCTGGAAT TGCTGGTAGT CCAGTGCAAG GAAGACACAC TATGGTGTCC
    CAGGGTACAC AAAGCAGTCC CATACTGAGC AGACAGTATC CTGTCACCCA GGGGATTCAG
    AGTAGTCCAA TTCTCAGCAG GCAGCCAATG GGACAAGCTG TCCAGAGCAG TCCAGTCCTC
    AACCGACAGG CATCCCTGAA TCAAGCAAAC CAAAGCAGTC CAGTGTTGAG TAGACAGCAG
    TCCATTACCC TACCCAACCA AGGCAGCCCG GTCCTAAGCA GACAGTCCTC GCTTAGTCAT
    CCCAGTCAGG GTAGTCCTGT ACTAGGACGC CACCCTTCCC TAACACAGGG TAGCCCCAGC
    CTGGATAGGC ATCCGATGTA CAGCGGTTAC ACCACTCCAG AAGAGCGACA CGGAGCTCTG
    TCACGCCAAA GCAGCTCTTC AGGATACCAG CCGCCATCTA CACCGTCTTT CCCAGTTTCT
    CCAGCAGGAT ACATCGAGGG AGCGGGATTC AGACAGGGGA GTCCTGCTTT CCAACCTCAG
    TTGCCCGAAA AGAGACGCAT GTCCAGCAGT GACCGCACCA ATGGTGGCCT GTCCTATGGG
    ACGCTCAATG GGAAGATGTC CTCACCGGTG TCCAGTGGCG GCAGCACTCC GAGTGTTCAT
    TTCTTCCACA CACTCCCCGA TTTCTCCAAA CTCAACATGT GTGATGGAAG TCCAGAAACC
    AGAATGAACG TGAAGTTTGT CCAGGACACG TCCAAATTCT GGTATAAACC AGATATCTCG
    AGAGACCAAG CGATTAACAT GCTGAAGGAT CAAGATCCTG GGGCCTTTGT GATTCGGGAC
    AGTCATTCAT TCAGAGGAGC CTACGGCTTG GCTATGAAAG TGGCTTCACC ACCTCCAACC
    ACTCAACCTA CAAAGAAAGC TGGAGATGTC ACTAATGAGT TGGTGAGGCA TTTTCTAATT
    GAGACGAGTC CGAAGGGTGT GAGACTGAAG GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC
    CTGTCTGCGC TGGTCTACCA GCACTCCATC ACTCCACTGG CACTGCCCTG CAAACTAGTC
    ATACCAACCA GAGATCCTCT GGAAGAGTCA CCTGAGATCG CAACACCAAC CAACCCGGCG
    GCAGATATGC TCAAGCAGGG CGCAGGGCAG AAGAGTTCAG CTGATGGCCA TGCATGCAAT
    GTGCTGTACA TCAACTCTGT GGATATGGAG TCTTTGACTG GACCTCAAGC CATCGCCAAA
    GCCATTTCAG AAACCATGAA CGCCAGTCCG GCACCCTCAG CCACCATTGT TCACTTCAAG
    GTGTCGGCAC AAGGCATCAC ACTCACAGAC AACCAGAGAA AGCTTTTCTT CAGACGTCAT
    TATCCCATCG GCACAGTCAC GTTCTGTGAC ATTGATCCAC AGGAGAGGAA GTGGAACAAA
    CCAGAGGGTG GCACTGCCAA ATTCTTTGGC TTTGTGGCTA GAAAGCAAGG CAGCACGACA
    GACAATGTCA GTCACCTGTT TGCTGAGATG GACCCAGATC AGCCGGCCAC CGCCATCGTC
    AACTTTGTCT CTAAAGTGAT GATCAGCTCT CAGAAGCGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213335.1
    CDS715..6096
    Translation

    Target ORF information:

    RefSeq Version XM_017357846.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357846.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
    TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG
    CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG
    GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG
    TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA
    CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG
    TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC
    ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC
    GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC
    TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT
    GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG
    GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA
    GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC
    GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG
    GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT
    AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC
    GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC
    ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA
    CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC
    CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT
    ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT
    GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT
    GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT
    TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA
    GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG
    GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT
    CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC
    ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT
    TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA
    GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT
    GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA
    GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT
    CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA
    GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG
    CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG
    TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA
    GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG
    AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC
    TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA
    GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT
    ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA
    CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC
    CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT
    TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT
    TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA
    GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG
    CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA
    CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA
    TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG
    GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC
    ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG
    CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT
    CCAATTGTTG GTGGTGAGGT CCTACCCCAA ACCCCTGCCT TCCCTGTCTC GCCACAAACC
    CCTTACTTCA ACATGTGTCG ATCTCCTCCA GGATTGGCTA AAACACCACT GTCCTTTCTG
    GGCCTGAAGC CCCACAACCC TGCTGAGATC CTGCTCAACC AGACAGGCTC AGAGAGTGAG
    GATGAGGAAG AGCCTCGTAG TTATGTGGAG TCTGTGGCGA AGGCAGCAGC AGTACCTGCA
    TCTCCAACAT ATGACAGCGA CAGCTTAGCT CGTCCTGCCG GACCGTCCCA TGCTTACAAT
    GCTCCACTTT CCTCCAGCAG CCCCATTCAG AACCCAGATC CAGTTGAAAG CACATTGGCT
    GACAGCGGGG TCGGGATGAA TTCTTTATCC CAGGCTGCTG TCGACATTTT CCATTTCCAG
    CCCTCCGAAA TCCCATACCA ACCACCACCA CCCCCCTACC CAACCTCCAT GTTGGGTAGC
    TACATCAGCC CTTCTCCAGC AGAGGCCATG CAAAATAATA CCACCACCAT CCAGCCACTT
    TCTGGAAGTC CTGGGACAAG ACACCACACC ATCCTTTCTG ACATGGCGTC CGTTCCCAAC
    TTTCAACACA GATTAGCAAA TCAGGATGGT CCCATACTTG GGCGACAGCA GGCTCCAGCT
    AATGGTCATG TGCCTGGAAT TGCTGGTAGT CCAGTGCAAG GAAGACACAC TATGGTGTCC
    CAGGGTACAC AAAGCAGTCC CATACTGAGC AGACAGTATC CTGTCACCCA GGGGATTCAG
    AGTAGTCCAA TTCTCAGCAG GCAGCCAATG GGACAAGCTG TCCAGAGCAG TCCAGTCCTC
    AACCGACAGG CATCCCTGAA TCAAGCAAAC CAAAGCAGTC CAGTGTTGAG TAGACAGCAG
    TCCATTACCC TACCCAACCA AGGCAGCCCG GTCCTAAGCA GACAGTCCTC GCTTAGTCAT
    CCCAGTCAGG GTAGTCCTGT ACTAGGACGC CACCCTTCCC TAACACAGGG TAGCCCCAGC
    CTGGATAGGC ATCCGATGTA CAGCGGTTAC ACCACTCCAG AAGAGCGACA CGGAGCTCTG
    TCACGCCAAA GCAGCTCTTC AGGATACCAG CCGCCATCTA CACCGTCTTT CCCAGTTTCT
    CCAGCAGGAT ACATCGAGGG AGCGGGATTC AGACAGGGGA GTCCTGCTTT CCAACCTCAG
    TTGCCCGAAA AGAGACGCAT GTCCAGCAGT GACCGCACCA ATGGTGGCCT GTCCTATGGG
    ACGCTCAATG GGAAGATGTC CTCACCGGTG TCCAGTGGCG GCAGCACTCC GAGTGTTCAT
    TTCTTCCACA CACTCCCCGA TTTCTCCAAA CTCAACATGT GTGATGGAAG TCCAGAAACC
    AGAATGAACG TGAAGTTTGT CCAGGACACG TCCAAATTCT GGTATAAACC AGATATCTCG
    AGAGACCAAG CGATTAACAT GCTGAAGGAT CAAGATCCTG GGGCCTTTGT GATTCGGGAC
    AGTCATTCAT TCAGAGGAGC CTACGGCTTG GCTATGAAAG TGGCTTCACC ACCTCCAACC
    ACTCAACCTA CAAAGAAAGC TGGAGATGTC ACTAATGAGT TGGTGAGGCA TTTTCTAATT
    GAGACGAGTC CGAAGGGTGT GAGACTGAAG GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC
    CTGTCTGCGC TGGTCTACCA GCACTCCATC ACTCCACTGG CACTGCCCTG CAAACTAGTC
    ATACCAACCA GAGATCCTCT GGAAGAGTCA CCTGAGATCG CAACACCAAC CAACCCGGCG
    GCAGATATGC TCAAGCAGGG CGCAGGGCAG AAGAGTTCAG CTGATGGCCA TGCATGCAAT
    GTGCTGTACA TCAACTCTGT GGATATGGAG TCTTTGACTG GACCTCAAGC CATCGCCAAA
    GCCATTTCAG AAACCATGAA CGCCAGTCCG GCACCCTCAG CCACCATTGT TCACTTCAAG
    GTGTCGGCAC AAGGCATCAC ACTCACAGAC AACCAGAGAA AGCTTTTCTT CAGACGTCAT
    TATCCCATCG GCACAGTCAC GTTCTGTGAC ATTGATCCAC AGGAGAGGAA GTGGAACAAA
    CCAGAGGGTG GCACTGCCAA ATTCTTTGGC TTTGTGGCTA GAAAGCAAGG CAGCACGACA
    GACAATGTCA GTCACCTGTT TGCTGAGATG GACCCAGATC AGCCGGCCAC CGCCATCGTC
    AACTTTGTCT CTAAAGTGAT GATCAGCTCT CAGAAGCGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60376
    Clone ID Related Accession (Same CDS sequence) XM_017357847.1
    Accession Version XM_017357847.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product tensin-1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCTTGAGT CCAGTTCTTC TTTGGGATTA AATATTCGGG GGCTAACAAG ACGAGTCGGT 
    GAGATGATGT CCGGCTGGAC GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG
    CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG
    GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG
    TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA
    CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG
    TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC
    ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC
    GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC
    TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT
    GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG
    GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA
    GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC
    GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG
    GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT
    AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC
    GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC
    ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA
    CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC
    CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT
    ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT
    GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT
    GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT
    TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA
    GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG
    GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT
    CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC
    ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT
    TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA
    GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT
    GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA
    GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT
    CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA
    GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG
    CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG
    TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA
    GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG
    AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC
    TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA
    GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT
    ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA
    CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC
    CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT
    TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT
    TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA
    GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG
    CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA
    CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA
    TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG
    GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC
    ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG
    CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT
    CCAATTGTTG GTGGTGAGGT CCTACCCCAA ACCCCTGCCT TCCCTGTCTC GCCACAAACC
    CCTTACTTCA ACATGTGTCG ATCTCCTCCA GGATTGGCTA AAACACCACT GTCCTTTCTG
    GGCCTGAAGC CCCACAACCC TGCTGAGATC CTGCTCAACC AGACAGGCTC AGAGAGTGAG
    GATGAGGAAG AGCCTCGTAG TTATGTGGAG TCTGTGGCGA AGGCAGCAGC AGTACCTGCA
    TCTCCAACAT ATGACAGCGA CAGCTTAGCT CGTCCTGCCG GACCGTCCCA TGCTTACAAT
    GCTCCACTTT CCTCCAGCAG CCCCATTCAG AACCCAGATC CAGTTGAAAG CACATTGGCT
    GACAGCGGGG TCGGGATGAA TTCTTTATCC CAGGCTGCTG TCGACATTTT CCATTTCCAG
    CCCTCCGAAA TCCCATACCA ACCACCACCA CCCCCCTACC CAACCTCCAT GTTGGGTAGC
    TACATCAGCC CTTCTCCAGC AGAGGCCATG CAAAATAATA CCACCACCAT CCAGCCACTT
    TCTGGAAGTC CTGGGACAAG ACACCACACC ATCCTTTCTG ACATGGCGTC CGTTCCCAAC
    TTTCAACACA GATTAGCAAA TCAGGATGGT CCCATACTTG GGCGACAGCA GGCTCCAGCT
    AATGGTCATG TGCCTGGAAT TGCTGGTAGT CCAGTGCAAG GAAGACACAC TATGGTGTCC
    CAGGGTACAC AAAGCAGTCC CATACTGAGC AGACAGTATC CTGTCACCCA GGGGATTCAG
    AGTAGTCCAA TTCTCAGCAG GCAGCCAATG GGACAAGCTG TCCAGAGCAG TCCAGTCCTC
    AACCGACAGG CATCCCTGAA TCAAGCAAAC CAAAGCAGTC CAGTGTTGAG TAGACAGCAG
    TCCATTACCC TACCCAACCA AGGCAGCCCG GTCCTAAGCA GACAGTCCTC GCTTAGTCAT
    CCCAGTCAGG GTAGTCCTGT ACTAGGACGC CACCCTTCCC TAACACAGGG TAGCCCCAGC
    CTGGATAGGC ATCCGATGTA CAGCGGTTAC ACCACTCCAG AAGAGCGACA CGGAGCTCTG
    TCACGCCAAA GCAGCTCTTC AGGATACCAG CCGCCATCTA CACCGTCTTT CCCAGTTTCT
    CCAGCAGGAT ACATCGAGGG AGCGGGATTC AGACAGGGGA GTCCTGCTTT CCAACCTCAG
    TTGCCCGAAA AGAGACGCAT GTCCAGCAGT GACCGCACCA ATGGTGGCCT GTCCTATGGG
    ACGCTCAATG GGAAGATGTC CTCACCGGTG TCCAGTGGCG GCAGCACTCC GAGTGTTCAT
    TTCTTCCACA CACTCCCCGA TTTCTCCAAA CTCAACATGT GTGATGGAAG TCCAGAAACC
    AGAATGAACG TGAAGTTTGT CCAGGACACG TCCAAATTCT GGTATAAACC AGATATCTCG
    AGAGACCAAG CGATTAACAT GCTGAAGGAT CAAGATCCTG GGGCCTTTGT GATTCGGGAC
    AGTCATTCAT TCAGAGGAGC CTACGGCTTG GCTATGAAAG TGGCTTCACC ACCTCCAACC
    ACTCAACCTA CAAAGAAAGC TGGAGATGTC ACTAATGAGT TGGTGAGGCA TTTTCTAATT
    GAGACGAGTC CGAAGGGTGT GAGACTGAAG GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC
    CTGTCTGCGC TGGTCTACCA GCACTCCATC ACTCCACTGG CACTGCCCTG CAAACTAGTC
    ATACCAACCA GAGATCCTCT GGAAGAGTCA CCTGAGATCG CAACACCAAC CAACCCGGCG
    GCAGATATGC TCAAGCAGGG CGCAGGGCAG AAGAGTTCAG CTGATGGCCA TGCATGCAAT
    GTGCTGTACA TCAACTCTGT GGATATGGAG TCTTTGACTG GACCTCAAGC CATCGCCAAA
    GCCATTTCAG AAACCATGAA CGCCAGTCCG GCACCCTCAG CCACCATTGT TCACTTCAAG
    GTGTCGGCAC AAGGCATCAC ACTCACAGAC AACCAGAGAA AGCTTTTCTT CAGACGTCAT
    TATCCCATCG GCACAGTCAC GTTCTGTGAC ATTGATCCAC AGGAGAGGAA GTGGAACAAA
    CCAGAGGGTG GCACTGCCAA ATTCTTTGGC TTTGTGGCTA GAAAGCAAGG CAGCACGACA
    GACAATGTCA GTCACCTGTT TGCTGAGATG GACCCAGATC AGCCGGCCAC CGCCATCGTC
    AACTTTGTCT CTAAAGTGAT GATCAGCTCT CAGAAGCGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213336.1
    CDS119..5500
    Translation

    Target ORF information:

    RefSeq Version XM_017357847.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357847.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCTTGAGT CCAGTTCTTC TTTGGGATTA AATATTCGGG GGCTAACAAG ACGAGTCGGT 
    GAGATGATGT CCGGCTGGAC GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG
    CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG
    GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG
    TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA
    CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG
    TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC
    ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC
    GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC
    TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT
    GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG
    GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA
    GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC
    GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG
    GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT
    AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC
    GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC
    ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA
    CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC
    CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT
    ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT
    GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT
    GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT
    TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA
    GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG
    GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT
    CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC
    ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT
    TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA
    GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT
    GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA
    GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT
    CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA
    GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG
    CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG
    TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA
    GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG
    AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC
    TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA
    GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT
    ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA
    CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC
    CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT
    TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT
    TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA
    GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG
    CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA
    CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA
    TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG
    GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC
    ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG
    CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT
    CCAATTGTTG GTGGTGAGGT CCTACCCCAA ACCCCTGCCT TCCCTGTCTC GCCACAAACC
    CCTTACTTCA ACATGTGTCG ATCTCCTCCA GGATTGGCTA AAACACCACT GTCCTTTCTG
    GGCCTGAAGC CCCACAACCC TGCTGAGATC CTGCTCAACC AGACAGGCTC AGAGAGTGAG
    GATGAGGAAG AGCCTCGTAG TTATGTGGAG TCTGTGGCGA AGGCAGCAGC AGTACCTGCA
    TCTCCAACAT ATGACAGCGA CAGCTTAGCT CGTCCTGCCG GACCGTCCCA TGCTTACAAT
    GCTCCACTTT CCTCCAGCAG CCCCATTCAG AACCCAGATC CAGTTGAAAG CACATTGGCT
    GACAGCGGGG TCGGGATGAA TTCTTTATCC CAGGCTGCTG TCGACATTTT CCATTTCCAG
    CCCTCCGAAA TCCCATACCA ACCACCACCA CCCCCCTACC CAACCTCCAT GTTGGGTAGC
    TACATCAGCC CTTCTCCAGC AGAGGCCATG CAAAATAATA CCACCACCAT CCAGCCACTT
    TCTGGAAGTC CTGGGACAAG ACACCACACC ATCCTTTCTG ACATGGCGTC CGTTCCCAAC
    TTTCAACACA GATTAGCAAA TCAGGATGGT CCCATACTTG GGCGACAGCA GGCTCCAGCT
    AATGGTCATG TGCCTGGAAT TGCTGGTAGT CCAGTGCAAG GAAGACACAC TATGGTGTCC
    CAGGGTACAC AAAGCAGTCC CATACTGAGC AGACAGTATC CTGTCACCCA GGGGATTCAG
    AGTAGTCCAA TTCTCAGCAG GCAGCCAATG GGACAAGCTG TCCAGAGCAG TCCAGTCCTC
    AACCGACAGG CATCCCTGAA TCAAGCAAAC CAAAGCAGTC CAGTGTTGAG TAGACAGCAG
    TCCATTACCC TACCCAACCA AGGCAGCCCG GTCCTAAGCA GACAGTCCTC GCTTAGTCAT
    CCCAGTCAGG GTAGTCCTGT ACTAGGACGC CACCCTTCCC TAACACAGGG TAGCCCCAGC
    CTGGATAGGC ATCCGATGTA CAGCGGTTAC ACCACTCCAG AAGAGCGACA CGGAGCTCTG
    TCACGCCAAA GCAGCTCTTC AGGATACCAG CCGCCATCTA CACCGTCTTT CCCAGTTTCT
    CCAGCAGGAT ACATCGAGGG AGCGGGATTC AGACAGGGGA GTCCTGCTTT CCAACCTCAG
    TTGCCCGAAA AGAGACGCAT GTCCAGCAGT GACCGCACCA ATGGTGGCCT GTCCTATGGG
    ACGCTCAATG GGAAGATGTC CTCACCGGTG TCCAGTGGCG GCAGCACTCC GAGTGTTCAT
    TTCTTCCACA CACTCCCCGA TTTCTCCAAA CTCAACATGT GTGATGGAAG TCCAGAAACC
    AGAATGAACG TGAAGTTTGT CCAGGACACG TCCAAATTCT GGTATAAACC AGATATCTCG
    AGAGACCAAG CGATTAACAT GCTGAAGGAT CAAGATCCTG GGGCCTTTGT GATTCGGGAC
    AGTCATTCAT TCAGAGGAGC CTACGGCTTG GCTATGAAAG TGGCTTCACC ACCTCCAACC
    ACTCAACCTA CAAAGAAAGC TGGAGATGTC ACTAATGAGT TGGTGAGGCA TTTTCTAATT
    GAGACGAGTC CGAAGGGTGT GAGACTGAAG GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC
    CTGTCTGCGC TGGTCTACCA GCACTCCATC ACTCCACTGG CACTGCCCTG CAAACTAGTC
    ATACCAACCA GAGATCCTCT GGAAGAGTCA CCTGAGATCG CAACACCAAC CAACCCGGCG
    GCAGATATGC TCAAGCAGGG CGCAGGGCAG AAGAGTTCAG CTGATGGCCA TGCATGCAAT
    GTGCTGTACA TCAACTCTGT GGATATGGAG TCTTTGACTG GACCTCAAGC CATCGCCAAA
    GCCATTTCAG AAACCATGAA CGCCAGTCCG GCACCCTCAG CCACCATTGT TCACTTCAAG
    GTGTCGGCAC AAGGCATCAC ACTCACAGAC AACCAGAGAA AGCTTTTCTT CAGACGTCAT
    TATCCCATCG GCACAGTCAC GTTCTGTGAC ATTGATCCAC AGGAGAGGAA GTGGAACAAA
    CCAGAGGGTG GCACTGCCAA ATTCTTTGGC TTTGTGGCTA GAAAGCAAGG CAGCACGACA
    GACAATGTCA GTCACCTGTT TGCTGAGATG GACCCAGATC AGCCGGCCAC CGCCATCGTC
    AACTTTGTCT CTAAAGTGAT GATCAGCTCT CAGAAGCGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60377
    Clone ID Related Accession (Same CDS sequence) XM_017357848.1
    Accession Version XM_017357848.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product tensin-1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
    TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG
    CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG
    GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG
    TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA
    CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG
    TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC
    ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC
    GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC
    TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT
    GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG
    GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA
    GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC
    GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG
    GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT
    AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC
    GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC
    ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA
    CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC
    CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT
    ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT
    GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT
    GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT
    TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA
    GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG
    GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT
    CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC
    ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT
    TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA
    GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT
    GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA
    GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT
    CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA
    GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG
    CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG
    TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA
    GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG
    AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC
    TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA
    GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT
    ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA
    CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC
    CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT
    TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT
    TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA
    GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG
    CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA
    CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA
    TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG
    GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC
    ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG
    CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT
    CCAATTGTTG GTGGTGAGGT CCTACCCCAA ACCCCTGCCT TCCCTGTCTC GCCACAAACC
    CCTTACTTCA ACATGTGTCG ATCTCCTCCA GGATTGGCTA AAACACCACT GTCCTTTCTG
    GGCCTGAAGC CCCACAACCC TGCTGAGATC CTGCTCAACC AGACAGGCTC AGAGCCTCGT
    AGTTATGTGG AGTCTGTGGC GAAGGCAGCA GCAGTACCTG CATCTCCAAC ATATGACAGC
    GACAGCTTAG CTCGTCCTGC CGGACCGTCC CATGCTTACA ATGCTCCACT TTCCTCCAGC
    AGCCCCATTC AGAACCCAGA TCCAGTTGAA AGCACATTGG CTGACAGCGG GGTCGGGATG
    AATTCTTTAT CCCAGGCTGC TGTCGACATT TTCCATTTCC AGCCCTCCGA AATCCCATAC
    CAACCACCAC CACCCCCCTA CCCAACCTCC ATGTTGGGTA GCTACATCAG CCCTTCTCCA
    GCAGAGGCCA TGCAAAATAA TACCACCACC ATCCAGCCAC TTTCTGGAAG TCCTGGGACA
    AGACACCACA CCATCCTTTC TGACATGGCG TCCGTTCCCA ACTTTCAACA CAGATTAGCA
    AATCAGGATG GTCCCATACT TGGGCGACAG CAGGCTCCAG CTAATGGTCA TGTGCCTGGA
    ATTGCTGGTA GTCCAGTGCA AGGAAGACAC ACTATGGTGT CCCAGGGTAC ACAAAGCAGT
    CCCATACTGA GCAGACAGTA TCCTGTCACC CAGGGGATTC AGAGTAGTCC AATTCTCAGC
    AGGCAGCCAA TGGGACAAGC TGTCCAGAGC AGTCCAGTCC TCAACCGACA GGCATCCCTG
    AATCAAGCAA ACCAAAGCAG TCCAGTGTTG AGTAGACAGC AGTCCATTAC CCTACCCAAC
    CAAGGCAGCC CGGTCCTAAG CAGACAGTCC TCGCTTAGTC ATCCCAGTCA GGGTAGTCCT
    GTACTAGGAC GCCACCCTTC CCTAACACAG GGTAGCCCCA GCCTGGATAG GCATCCGATG
    TACAGCGGTT ACACCACTCC AGAAGAGCGA CACGGAGCTC TGTCACGCCA AAGCAGCTCT
    TCAGGATACC AGCCGCCATC TACACCGTCT TTCCCAGTTT CTCCAGCAGG ATACATCGAG
    GGAGCGGGAT TCAGACAGGG GAGTCCTGCT TTCCAACCTC AGTTGCCCGA AAAGAGACGC
    ATGTCCAGCA GTGACCGCAC CAATGGTGGC CTGTCCTATG GGACGCTCAA TGGGAAGATG
    TCCTCACCGG TGTCCAGTGG CGGCAGCACT CCGAGTGTTC ATTTCTTCCA CACACTCCCC
    GATTTCTCCA AACTCAACAT GTGTGATGGA AGTCCAGAAA CCAGAATGAA CGTGAAGTTT
    GTCCAGGACA CGTCCAAATT CTGGTATAAA CCAGATATCT CGAGAGACCA AGCGATTAAC
    ATGCTGAAGG ATCAAGATCC TGGGGCCTTT GTGATTCGGG ACAGTCATTC ATTCAGAGGA
    GCCTACGGCT TGGCTATGAA AGTGGCTTCA CCACCTCCAA CCACTCAACC TACAAAGAAA
    GCTGGAGATG TCACTAATGA GTTGGTGAGG CATTTTCTAA TTGAGACGAG TCCGAAGGGT
    GTGAGACTGA AGGGCTGCCC CAACGAGCCG TACTTTGGCT GCCTGTCTGC GCTGGTCTAC
    CAGCACTCCA TCACTCCACT GGCACTGCCC TGCAAACTAG TCATACCAAC CAGAGATCCT
    CTGGAAGAGT CACCTGAGAT CGCAACACCA ACCAACCCGG CGGCAGATAT GCTCAAGCAG
    GGCGCAGGGC AGAAGAGTTC AGCTGATGGC CATGCATGCA ATGTGCTGTA CATCAACTCT
    GTGGATATGG AGTCTTTGAC TGGACCTCAA GCCATCGCCA AAGCCATTTC AGAAACCATG
    AACGCCAGTC CGGCACCCTC AGCCACCATT GTTCACTTCA AGGTGTCGGC ACAAGGCATC
    ACACTCACAG ACAACCAGAG AAAGCTTTTC TTCAGACGTC ATTATCCCAT CGGCACAGTC
    ACGTTCTGTG ACATTGATCC ACAGGAGAGG AAGTGGAACA AACCAGAGGG TGGCACTGCC
    AAATTCTTTG GCTTTGTGGC TAGAAAGCAA GGCAGCACGA CAGACAATGT CAGTCACCTG
    TTTGCTGAGA TGGACCCAGA TCAGCCGGCC ACCGCCATCG TCAACTTTGT CTCTAAAGTG
    ATGATCAGCT CTCAGAAGCG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213337.1
    CDS714..6077
    Translation

    Target ORF information:

    RefSeq Version XM_017357848.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
    TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG
    CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG
    GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG
    TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA
    CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG
    TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC
    ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC
    GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC
    TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT
    GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG
    GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA
    GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC
    GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG
    GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT
    AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC
    GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC
    ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA
    CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC
    CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT
    ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT
    GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT
    GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT
    TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA
    GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG
    GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT
    CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC
    ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT
    TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA
    GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT
    GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA
    GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT
    CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA
    GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG
    CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG
    TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA
    GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG
    AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC
    TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA
    GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT
    ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA
    CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC
    CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT
    TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT
    TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA
    GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG
    CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA
    CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA
    TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG
    GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC
    ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG
    CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT
    CCAATTGTTG GTGGTGAGGT CCTACCCCAA ACCCCTGCCT TCCCTGTCTC GCCACAAACC
    CCTTACTTCA ACATGTGTCG ATCTCCTCCA GGATTGGCTA AAACACCACT GTCCTTTCTG
    GGCCTGAAGC CCCACAACCC TGCTGAGATC CTGCTCAACC AGACAGGCTC AGAGCCTCGT
    AGTTATGTGG AGTCTGTGGC GAAGGCAGCA GCAGTACCTG CATCTCCAAC ATATGACAGC
    GACAGCTTAG CTCGTCCTGC CGGACCGTCC CATGCTTACA ATGCTCCACT TTCCTCCAGC
    AGCCCCATTC AGAACCCAGA TCCAGTTGAA AGCACATTGG CTGACAGCGG GGTCGGGATG
    AATTCTTTAT CCCAGGCTGC TGTCGACATT TTCCATTTCC AGCCCTCCGA AATCCCATAC
    CAACCACCAC CACCCCCCTA CCCAACCTCC ATGTTGGGTA GCTACATCAG CCCTTCTCCA
    GCAGAGGCCA TGCAAAATAA TACCACCACC ATCCAGCCAC TTTCTGGAAG TCCTGGGACA
    AGACACCACA CCATCCTTTC TGACATGGCG TCCGTTCCCA ACTTTCAACA CAGATTAGCA
    AATCAGGATG GTCCCATACT TGGGCGACAG CAGGCTCCAG CTAATGGTCA TGTGCCTGGA
    ATTGCTGGTA GTCCAGTGCA AGGAAGACAC ACTATGGTGT CCCAGGGTAC ACAAAGCAGT
    CCCATACTGA GCAGACAGTA TCCTGTCACC CAGGGGATTC AGAGTAGTCC AATTCTCAGC
    AGGCAGCCAA TGGGACAAGC TGTCCAGAGC AGTCCAGTCC TCAACCGACA GGCATCCCTG
    AATCAAGCAA ACCAAAGCAG TCCAGTGTTG AGTAGACAGC AGTCCATTAC CCTACCCAAC
    CAAGGCAGCC CGGTCCTAAG CAGACAGTCC TCGCTTAGTC ATCCCAGTCA GGGTAGTCCT
    GTACTAGGAC GCCACCCTTC CCTAACACAG GGTAGCCCCA GCCTGGATAG GCATCCGATG
    TACAGCGGTT ACACCACTCC AGAAGAGCGA CACGGAGCTC TGTCACGCCA AAGCAGCTCT
    TCAGGATACC AGCCGCCATC TACACCGTCT TTCCCAGTTT CTCCAGCAGG ATACATCGAG
    GGAGCGGGAT TCAGACAGGG GAGTCCTGCT TTCCAACCTC AGTTGCCCGA AAAGAGACGC
    ATGTCCAGCA GTGACCGCAC CAATGGTGGC CTGTCCTATG GGACGCTCAA TGGGAAGATG
    TCCTCACCGG TGTCCAGTGG CGGCAGCACT CCGAGTGTTC ATTTCTTCCA CACACTCCCC
    GATTTCTCCA AACTCAACAT GTGTGATGGA AGTCCAGAAA CCAGAATGAA CGTGAAGTTT
    GTCCAGGACA CGTCCAAATT CTGGTATAAA CCAGATATCT CGAGAGACCA AGCGATTAAC
    ATGCTGAAGG ATCAAGATCC TGGGGCCTTT GTGATTCGGG ACAGTCATTC ATTCAGAGGA
    GCCTACGGCT TGGCTATGAA AGTGGCTTCA CCACCTCCAA CCACTCAACC TACAAAGAAA
    GCTGGAGATG TCACTAATGA GTTGGTGAGG CATTTTCTAA TTGAGACGAG TCCGAAGGGT
    GTGAGACTGA AGGGCTGCCC CAACGAGCCG TACTTTGGCT GCCTGTCTGC GCTGGTCTAC
    CAGCACTCCA TCACTCCACT GGCACTGCCC TGCAAACTAG TCATACCAAC CAGAGATCCT
    CTGGAAGAGT CACCTGAGAT CGCAACACCA ACCAACCCGG CGGCAGATAT GCTCAAGCAG
    GGCGCAGGGC AGAAGAGTTC AGCTGATGGC CATGCATGCA ATGTGCTGTA CATCAACTCT
    GTGGATATGG AGTCTTTGAC TGGACCTCAA GCCATCGCCA AAGCCATTTC AGAAACCATG
    AACGCCAGTC CGGCACCCTC AGCCACCATT GTTCACTTCA AGGTGTCGGC ACAAGGCATC
    ACACTCACAG ACAACCAGAG AAAGCTTTTC TTCAGACGTC ATTATCCCAT CGGCACAGTC
    ACGTTCTGTG ACATTGATCC ACAGGAGAGG AAGTGGAACA AACCAGAGGG TGGCACTGCC
    AAATTCTTTG GCTTTGTGGC TAGAAAGCAA GGCAGCACGA CAGACAATGT CAGTCACCTG
    TTTGCTGAGA TGGACCCAGA TCAGCCGGCC ACCGCCATCG TCAACTTTGT CTCTAAAGTG
    ATGATCAGCT CTCAGAAGCG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60378
    Clone ID Related Accession (Same CDS sequence) XM_017357849.1
    Accession Version XM_017357849.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product tensin-1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
    TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG
    CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG
    GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG
    TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA
    CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG
    TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC
    ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC
    GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC
    TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT
    GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG
    GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA
    GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC
    GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG
    GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT
    AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC
    GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC
    ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA
    CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC
    CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT
    ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT
    GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT
    GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT
    TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA
    GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG
    GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT
    CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC
    ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT
    TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA
    GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT
    GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA
    GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT
    CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA
    GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG
    CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG
    TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA
    GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG
    AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC
    TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA
    GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT
    ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA
    CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC
    CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT
    TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT
    TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA
    GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG
    CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA
    CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA
    TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG
    GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC
    ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG
    CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT
    CCAATTGTTG GTGGTGAGGT CCTACCCCAA ACCCCTGCCT TCCCTGTCTC GCCACAAACC
    CCTTACTTCA ACATGTGTCG ATCTCCTCCA GGATTGGCTA AAACACCACT GTCCTTTCTG
    GGCCTGAAGC CCCACAACCC TGCTGAGATC CTGCTCAACC AGACAGGCTC AGAGAGTGAG
    GATGAGGAAG AGCCTCGTAG TTATGTGGAG TCTGTGGCGA AGGCAGCAGC AGTACCTGCA
    TCTCCAACAT ATGACAGCGA CAGCTTAGCT CGTCCTGCCG GACCGTCCCA TGCTTACAAT
    GCTCCACTTT CCTCCAGCAG CCCCATTCAG AACCCAGATC CAGTTGAAAG CACATTGGCT
    GACAGCGGGG TCGGGATGAA TTCTTTATCC CAGGCTGCTG TCGACATTTT CCATTTCCAG
    CCCTCCGAAA TCCCATACCA ACCACCACCA CCCCCCTACC CAACCTCCAT GTTGGGTAGC
    TACATCAGCC CTTCTCCAGC AGAGGCCATG CAAAATAATA CCACCACCAT CCAGCCACTT
    TCTGGAAGTC CTGGGACAAG ACACCACACC ATCCTTTCTG ACATGGCGTC CGTTCCCAAC
    TTTCAACACA GATTAGCAAA TCAGGATGGT CCCATACTTG GGCGACAGCA GGCTCCAGCT
    AATGGTCATG TGCCTGGAAT TGCTGGTAGT CCAGTGCAAG GAAGACACAC TATGGTGTCC
    CAGGGTACAC AAAGCAGTCC CATACTGAGC AGACAGTATC CTGTCACCCA GGGGATTCAG
    AGTAGTCCAA TTCTCAGCAG GCAGCCAATG GGACAAGCTG TCCAGAGCAG TCCAGTCCTC
    AACCGACAGG CATCCCTGAA TCAAGCAAAC CAAAGCAGTC CAGTGTTGAG TAGACAGCAG
    TCCATTACCC TACCCAACCA AGGCAGCCCG GTCCTAAGCA GACAGTCCTC GCTTAGTCAT
    CCCAGTCAGG GTAGTCCTGT ACTAGGACGC CACCCTTCCC TAACACAGGG TAGCCCCAGC
    CTGGATAGGC ATCCGATGTA CAGCGGTTAC ACCACTCCAG AAGAGCGACA CGGAGCTCTG
    TCACGCCAAA GCAGCTCTTC AGGATACCAG CCGCCATCTA CACCGTCTTT CCCAGTTTCT
    CCAGCAGGAT ACATCGAGGG AGCGGGATTC AGACAGGGGA GTCCTGCTTT CCAACCTCAG
    TTGCCCGAAA AGAGACGCAT GTCCAGCAGT GACCGCACCA ATGGTGGCCT GTCCTATGGG
    ACGCTCAATG GGAAGATGTC CTCACCGGTG TCCAGTGGCG GCAGCACTCC GAGTGTTCAT
    TTCTTCCACA CACTCCCCGA TTTCTCCAAA CTCAACATGT GTGATGGAAG TCCAGAAACC
    AGAATGAACG TGAAGTTTGT CCAGGACACG TCCAAATTCT GGTATAAACC AGATATCTCG
    AGAGACCAAG CGATTAACAT GCTGAAGGAT CAAGATCCTG GGGCCTTTGT GATTCGGGAC
    AGTCATTCAT TCAGAGGAGC CTACGGCTTG GCTATGAAAG TGGCTTCACC ACCTCCAACC
    ACTCAACCTA CAAAGAAAGC TGGAGATGTC ACTAATGAGT TGGTGAGGCA TTTTCTAATT
    GAGACGAGTC CGAAGGGTGT GAGACTGAAG GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC
    CTGTCTGCGC TGGTCTACCA GCACTCCATC ACTCCACTGG CACTGCCCTG CAAACTAGTC
    ATACCAACCA GAGATCCTCT GGAAGAGTCA CCTGAGATCG CAACACCAAC CAACCCGGCG
    GCAGATATGC TCAAGCAGGG CGCAGCATGC AATGTGCTGT ACATCAACTC TGTGGATATG
    GAGTCTTTGA CTGGACCTCA AGCCATCGCC AAAGCCATTT CAGAAACCAT GAACGCCAGT
    CCGGCACCCT CAGCCACCAT TGTTCACTTC AAGGTGTCGG CACAAGGCAT CACACTCACA
    GACAACCAGA GAAAGCTTTT CTTCAGACGT CATTATCCCA TCGGCACAGT CACGTTCTGT
    GACATTGATC CACAGGAGAG GAAGTGGAAC AAACCAGAGG GTGGCACTGC CAAATTCTTT
    GGCTTTGTGG CTAGAAAGCA AGGCAGCACG ACAGACAATG TCAGTCACCT GTTTGCTGAG
    ATGGACCCAG ATCAGCCGGC CACCGCCATC GTCAACTTTG TCTCTAAAGT GATGATCAGC
    TCTCAGAAGC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213338.1
    CDS714..6068
    Translation

    Target ORF information:

    RefSeq Version XM_017357849.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357849.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
    TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG
    CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG
    GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG
    TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA
    CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG
    TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC
    ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC
    GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC
    TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT
    GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG
    GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA
    GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC
    GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG
    GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT
    AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC
    GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC
    ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA
    CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC
    CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT
    ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT
    GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT
    GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT
    TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA
    GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG
    GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT
    CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC
    ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT
    TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA
    GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT
    GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA
    GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT
    CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA
    GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG
    CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG
    TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA
    GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG
    AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC
    TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA
    GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT
    ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA
    CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC
    CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT
    TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT
    TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA
    GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG
    CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA
    CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA
    TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG
    GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC
    ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG
    CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT
    CCAATTGTTG GTGGTGAGGT CCTACCCCAA ACCCCTGCCT TCCCTGTCTC GCCACAAACC
    CCTTACTTCA ACATGTGTCG ATCTCCTCCA GGATTGGCTA AAACACCACT GTCCTTTCTG
    GGCCTGAAGC CCCACAACCC TGCTGAGATC CTGCTCAACC AGACAGGCTC AGAGAGTGAG
    GATGAGGAAG AGCCTCGTAG TTATGTGGAG TCTGTGGCGA AGGCAGCAGC AGTACCTGCA
    TCTCCAACAT ATGACAGCGA CAGCTTAGCT CGTCCTGCCG GACCGTCCCA TGCTTACAAT
    GCTCCACTTT CCTCCAGCAG CCCCATTCAG AACCCAGATC CAGTTGAAAG CACATTGGCT
    GACAGCGGGG TCGGGATGAA TTCTTTATCC CAGGCTGCTG TCGACATTTT CCATTTCCAG
    CCCTCCGAAA TCCCATACCA ACCACCACCA CCCCCCTACC CAACCTCCAT GTTGGGTAGC
    TACATCAGCC CTTCTCCAGC AGAGGCCATG CAAAATAATA CCACCACCAT CCAGCCACTT
    TCTGGAAGTC CTGGGACAAG ACACCACACC ATCCTTTCTG ACATGGCGTC CGTTCCCAAC
    TTTCAACACA GATTAGCAAA TCAGGATGGT CCCATACTTG GGCGACAGCA GGCTCCAGCT
    AATGGTCATG TGCCTGGAAT TGCTGGTAGT CCAGTGCAAG GAAGACACAC TATGGTGTCC
    CAGGGTACAC AAAGCAGTCC CATACTGAGC AGACAGTATC CTGTCACCCA GGGGATTCAG
    AGTAGTCCAA TTCTCAGCAG GCAGCCAATG GGACAAGCTG TCCAGAGCAG TCCAGTCCTC
    AACCGACAGG CATCCCTGAA TCAAGCAAAC CAAAGCAGTC CAGTGTTGAG TAGACAGCAG
    TCCATTACCC TACCCAACCA AGGCAGCCCG GTCCTAAGCA GACAGTCCTC GCTTAGTCAT
    CCCAGTCAGG GTAGTCCTGT ACTAGGACGC CACCCTTCCC TAACACAGGG TAGCCCCAGC
    CTGGATAGGC ATCCGATGTA CAGCGGTTAC ACCACTCCAG AAGAGCGACA CGGAGCTCTG
    TCACGCCAAA GCAGCTCTTC AGGATACCAG CCGCCATCTA CACCGTCTTT CCCAGTTTCT
    CCAGCAGGAT ACATCGAGGG AGCGGGATTC AGACAGGGGA GTCCTGCTTT CCAACCTCAG
    TTGCCCGAAA AGAGACGCAT GTCCAGCAGT GACCGCACCA ATGGTGGCCT GTCCTATGGG
    ACGCTCAATG GGAAGATGTC CTCACCGGTG TCCAGTGGCG GCAGCACTCC GAGTGTTCAT
    TTCTTCCACA CACTCCCCGA TTTCTCCAAA CTCAACATGT GTGATGGAAG TCCAGAAACC
    AGAATGAACG TGAAGTTTGT CCAGGACACG TCCAAATTCT GGTATAAACC AGATATCTCG
    AGAGACCAAG CGATTAACAT GCTGAAGGAT CAAGATCCTG GGGCCTTTGT GATTCGGGAC
    AGTCATTCAT TCAGAGGAGC CTACGGCTTG GCTATGAAAG TGGCTTCACC ACCTCCAACC
    ACTCAACCTA CAAAGAAAGC TGGAGATGTC ACTAATGAGT TGGTGAGGCA TTTTCTAATT
    GAGACGAGTC CGAAGGGTGT GAGACTGAAG GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC
    CTGTCTGCGC TGGTCTACCA GCACTCCATC ACTCCACTGG CACTGCCCTG CAAACTAGTC
    ATACCAACCA GAGATCCTCT GGAAGAGTCA CCTGAGATCG CAACACCAAC CAACCCGGCG
    GCAGATATGC TCAAGCAGGG CGCAGCATGC AATGTGCTGT ACATCAACTC TGTGGATATG
    GAGTCTTTGA CTGGACCTCA AGCCATCGCC AAAGCCATTT CAGAAACCAT GAACGCCAGT
    CCGGCACCCT CAGCCACCAT TGTTCACTTC AAGGTGTCGG CACAAGGCAT CACACTCACA
    GACAACCAGA GAAAGCTTTT CTTCAGACGT CATTATCCCA TCGGCACAGT CACGTTCTGT
    GACATTGATC CACAGGAGAG GAAGTGGAAC AAACCAGAGG GTGGCACTGC CAAATTCTTT
    GGCTTTGTGG CTAGAAAGCA AGGCAGCACG ACAGACAATG TCAGTCACCT GTTTGCTGAG
    ATGGACCCAG ATCAGCCGGC CACCGCCATC GTCAACTTTG TCTCTAAAGT GATGATCAGC
    TCTCAGAAGC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60379
    Clone ID Related Accession (Same CDS sequence) XM_009304969.2
    Accession Version XM_009304969.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product tensin-1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
    TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG
    CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG
    GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG
    TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA
    CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG
    TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC
    ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC
    GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC
    TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT
    GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG
    GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA
    GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC
    GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG
    GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT
    AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC
    GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC
    ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA
    CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC
    CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT
    ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT
    GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT
    GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT
    TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA
    GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG
    GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT
    CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC
    ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT
    TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA
    GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT
    GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA
    GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT
    CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA
    GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG
    CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG
    TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA
    GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG
    AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC
    TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA
    GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT
    ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA
    CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC
    CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT
    TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT
    TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA
    GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG
    CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA
    CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA
    TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG
    GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC
    ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG
    CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT
    CCAATTGTTG GTGGTCGATC TCCTCCAGGA TTGGCTAAAA CACCACTGTC CTTTCTGGGC
    CTGAAGCCCC ACAACCCTGC TGAGATCCTG CTCAACCAGA CAGGCTCAGA GAGTGAGGAT
    GAGGAAGAGC CTCGTAGTTA TGTGGAGTCT GTGGCGAAGG CAGCAGCAGT ACCTGCATCT
    CCAACATATG ACAGCGACAG CTTAGCTCGT CCTGCCGGAC CGTCCCATGC TTACAATGCT
    CCACTTTCCT CCAGCAGCCC CATTCAGAAC CCAGATCCAG TTGAAAGCAC ATTGGCTGAC
    AGCGGGGTCG GGATGAATTC TTTATCCCAG GCTGCTGTCG ACATTTTCCA TTTCCAGCCC
    TCCGAAATCC CATACCAACC ACCACCACCC CCCTACCCAA CCTCCATGTT GGGTAGCTAC
    ATCAGCCCTT CTCCAGCAGA GGCCATGCAA AATAATACCA CCACCATCCA GCCACTTTCT
    GGAAGTCCTG GGACAAGACA CCACACCATC CTTTCTGACA TGGCGTCCGT TCCCAACTTT
    CAACACAGAT TAGCAAATCA GGATGGTCCC ATACTTGGGC GACAGCAGGC TCCAGCTAAT
    GGTCATGTGC CTGGAATTGC TGGTAGTCCA GTGCAAGGAA GACACACTAT GGTGTCCCAG
    GGTACACAAA GCAGTCCCAT ACTGAGCAGA CAGTATCCTG TCACCCAGGG GATTCAGAGT
    AGTCCAATTC TCAGCAGGCA GCCAATGGGA CAAGCTGTCC AGAGCAGTCC AGTCCTCAAC
    CGACAGGCAT CCCTGAATCA AGCAAACCAA AGCAGTCCAG TGTTGAGTAG ACAGCAGTCC
    ATTACCCTAC CCAACCAAGG CAGCCCGGTC CTAAGCAGAC AGTCCTCGCT TAGTCATCCC
    AGTCAGGGTA GTCCTGTACT AGGACGCCAC CCTTCCCTAA CACAGGGTAG CCCCAGCCTG
    GATAGGCATC CGATGTACAG CGGTTACACC ACTCCAGAAG AGCGACACGG AGCTCTGTCA
    CGCCAAAGCA GCTCTTCAGG ATACCAGCCG CCATCTACAC CGTCTTTCCC AGTTTCTCCA
    GCAGGATACA TCGAGGGAGC GGGATTCAGA CAGGGGAGTC CTGCTTTCCA ACCTCAGTTG
    CCCGAAAAGA GACGCATGTC CAGCAGTGAC CGCACCAATG GTGGCCTGTC CTATGGGACG
    CTCAATGGGA AGATGTCCTC ACCGGTGTCC AGTGGCGGCA GCACTCCGAG TGTTCATTTC
    TTCCACACAC TCCCCGATTT CTCCAAACTC AACATGTGTG ATGGAAGTCC AGAAACCAGA
    ATGAACGTGA AGTTTGTCCA GGACACGTCC AAATTCTGGT ATAAACCAGA TATCTCGAGA
    GACCAAGCGA TTAACATGCT GAAGGATCAA GATCCTGGGG CCTTTGTGAT TCGGGACAGT
    CATTCATTCA GAGGAGCCTA CGGCTTGGCT ATGAAAGTGG CTTCACCACC TCCAACCACT
    CAACCTACAA AGAAAGCTGG AGATGTCACT AATGAGTTGG TGAGGCATTT TCTAATTGAG
    ACGAGTCCGA AGGGTGTGAG ACTGAAGGGC TGCCCCAACG AGCCGTACTT TGGCTGCCTG
    TCTGCGCTGG TCTACCAGCA CTCCATCACT CCACTGGCAC TGCCCTGCAA ACTAGTCATA
    CCAACCAGAG ATCCTCTGGA AGAGTCACCT GAGATCGCAA CACCAACCAA CCCGGCGGCA
    GATATGCTCA AGCAGGGCGC AGGGCAGAAG AGTTCAGCTG ATGGCCATGC ATGCAATGTG
    CTGTACATCA ACTCTGTGGA TATGGAGTCT TTGACTGGAC CTCAAGCCAT CGCCAAAGCC
    ATTTCAGAAA CCATGAACGC CAGTCCGGCA CCCTCAGCCA CCATTGTTCA CTTCAAGGTG
    TCGGCACAAG GCATCACACT CACAGACAAC CAGAGAAAGC TTTTCTTCAG ACGTCATTAT
    CCCATCGGCA CAGTCACGTT CTGTGACATT GATCCACAGG AGAGGAAGTG GAACAAACCA
    GAGGGTGGCA CTGCCAAATT CTTTGGCTTT GTGGCTAGAA AGCAAGGCAG CACGACAGAC
    AATGTCAGTC ACCTGTTTGC TGAGATGGAC CCAGATCAGC CGGCCACCGC CATCGTCAAC
    TTTGTCTCTA AAGTGATGAT CAGCTCTCAG AAGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303244.2
    CDS715..6033
    Translation

    Target ORF information:

    RefSeq Version XM_009304969.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304969.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
    TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG
    CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG
    GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG
    TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA
    CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG
    TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC
    ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC
    GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC
    TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT
    GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG
    GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA
    GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC
    GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG
    GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT
    AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC
    GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC
    ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA
    CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC
    CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT
    ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT
    GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT
    GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT
    TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA
    GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG
    GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT
    CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC
    ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT
    TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA
    GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT
    GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA
    GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT
    CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA
    GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG
    CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG
    TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA
    GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG
    AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC
    TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA
    GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT
    ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA
    CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC
    CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT
    TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT
    TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA
    GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG
    CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA
    CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA
    TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG
    GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC
    ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG
    CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT
    CCAATTGTTG GTGGTCGATC TCCTCCAGGA TTGGCTAAAA CACCACTGTC CTTTCTGGGC
    CTGAAGCCCC ACAACCCTGC TGAGATCCTG CTCAACCAGA CAGGCTCAGA GAGTGAGGAT
    GAGGAAGAGC CTCGTAGTTA TGTGGAGTCT GTGGCGAAGG CAGCAGCAGT ACCTGCATCT
    CCAACATATG ACAGCGACAG CTTAGCTCGT CCTGCCGGAC CGTCCCATGC TTACAATGCT
    CCACTTTCCT CCAGCAGCCC CATTCAGAAC CCAGATCCAG TTGAAAGCAC ATTGGCTGAC
    AGCGGGGTCG GGATGAATTC TTTATCCCAG GCTGCTGTCG ACATTTTCCA TTTCCAGCCC
    TCCGAAATCC CATACCAACC ACCACCACCC CCCTACCCAA CCTCCATGTT GGGTAGCTAC
    ATCAGCCCTT CTCCAGCAGA GGCCATGCAA AATAATACCA CCACCATCCA GCCACTTTCT
    GGAAGTCCTG GGACAAGACA CCACACCATC CTTTCTGACA TGGCGTCCGT TCCCAACTTT
    CAACACAGAT TAGCAAATCA GGATGGTCCC ATACTTGGGC GACAGCAGGC TCCAGCTAAT
    GGTCATGTGC CTGGAATTGC TGGTAGTCCA GTGCAAGGAA GACACACTAT GGTGTCCCAG
    GGTACACAAA GCAGTCCCAT ACTGAGCAGA CAGTATCCTG TCACCCAGGG GATTCAGAGT
    AGTCCAATTC TCAGCAGGCA GCCAATGGGA CAAGCTGTCC AGAGCAGTCC AGTCCTCAAC
    CGACAGGCAT CCCTGAATCA AGCAAACCAA AGCAGTCCAG TGTTGAGTAG ACAGCAGTCC
    ATTACCCTAC CCAACCAAGG CAGCCCGGTC CTAAGCAGAC AGTCCTCGCT TAGTCATCCC
    AGTCAGGGTA GTCCTGTACT AGGACGCCAC CCTTCCCTAA CACAGGGTAG CCCCAGCCTG
    GATAGGCATC CGATGTACAG CGGTTACACC ACTCCAGAAG AGCGACACGG AGCTCTGTCA
    CGCCAAAGCA GCTCTTCAGG ATACCAGCCG CCATCTACAC CGTCTTTCCC AGTTTCTCCA
    GCAGGATACA TCGAGGGAGC GGGATTCAGA CAGGGGAGTC CTGCTTTCCA ACCTCAGTTG
    CCCGAAAAGA GACGCATGTC CAGCAGTGAC CGCACCAATG GTGGCCTGTC CTATGGGACG
    CTCAATGGGA AGATGTCCTC ACCGGTGTCC AGTGGCGGCA GCACTCCGAG TGTTCATTTC
    TTCCACACAC TCCCCGATTT CTCCAAACTC AACATGTGTG ATGGAAGTCC AGAAACCAGA
    ATGAACGTGA AGTTTGTCCA GGACACGTCC AAATTCTGGT ATAAACCAGA TATCTCGAGA
    GACCAAGCGA TTAACATGCT GAAGGATCAA GATCCTGGGG CCTTTGTGAT TCGGGACAGT
    CATTCATTCA GAGGAGCCTA CGGCTTGGCT ATGAAAGTGG CTTCACCACC TCCAACCACT
    CAACCTACAA AGAAAGCTGG AGATGTCACT AATGAGTTGG TGAGGCATTT TCTAATTGAG
    ACGAGTCCGA AGGGTGTGAG ACTGAAGGGC TGCCCCAACG AGCCGTACTT TGGCTGCCTG
    TCTGCGCTGG TCTACCAGCA CTCCATCACT CCACTGGCAC TGCCCTGCAA ACTAGTCATA
    CCAACCAGAG ATCCTCTGGA AGAGTCACCT GAGATCGCAA CACCAACCAA CCCGGCGGCA
    GATATGCTCA AGCAGGGCGC AGGGCAGAAG AGTTCAGCTG ATGGCCATGC ATGCAATGTG
    CTGTACATCA ACTCTGTGGA TATGGAGTCT TTGACTGGAC CTCAAGCCAT CGCCAAAGCC
    ATTTCAGAAA CCATGAACGC CAGTCCGGCA CCCTCAGCCA CCATTGTTCA CTTCAAGGTG
    TCGGCACAAG GCATCACACT CACAGACAAC CAGAGAAAGC TTTTCTTCAG ACGTCATTAT
    CCCATCGGCA CAGTCACGTT CTGTGACATT GATCCACAGG AGAGGAAGTG GAACAAACCA
    GAGGGTGGCA CTGCCAAATT CTTTGGCTTT GTGGCTAGAA AGCAAGGCAG CACGACAGAC
    AATGTCAGTC ACCTGTTTGC TGAGATGGAC CCAGATCAGC CGGCCACCGC CATCGTCAAC
    TTTGTCTCTA AAGTGATGAT CAGCTCTCAG AAGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60380
    Clone ID Related Accession (Same CDS sequence) XM_005167931.3
    Accession Version XM_005167931.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product tensin-1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167931.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
    TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG
    CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG
    GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG
    TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA
    CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG
    TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC
    ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC
    GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC
    TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT
    GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG
    GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA
    GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC
    GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG
    GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT
    AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC
    GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC
    ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA
    CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC
    CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT
    ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT
    GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT
    GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT
    TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA
    GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG
    GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT
    CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC
    ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT
    TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA
    GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT
    GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA
    GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT
    CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA
    GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG
    CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG
    TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA
    GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG
    AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC
    TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA
    GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT
    ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA
    CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC
    CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT
    TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT
    TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA
    GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG
    CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA
    CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA
    TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG
    GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC
    ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG
    CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT
    CCAATTGTTG GTGGTCGATC TCCTCCAGGA TTGGCTAAAA CACCACTGTC CTTTCTGGGC
    CTGAAGCCCC ACAACCCTGC TGAGATCCTG CTCAACCAGA CAGGCTCAGA GCCTCGTAGT
    TATGTGGAGT CTGTGGCGAA GGCAGCAGCA GTACCTGCAT CTCCAACATA TGACAGCGAC
    AGCTTAGCTC GTCCTGCCGG ACCGTCCCAT GCTTACAATG CTCCACTTTC CTCCAGCAGC
    CCCATTCAGA ACCCAGATCC AGTTGAAAGC ACATTGGCTG ACAGCGGGGT CGGGATGAAT
    TCTTTATCCC AGGCTGCTGT CGACATTTTC CATTTCCAGC CCTCCGAAAT CCCATACCAA
    CCACCACCAC CCCCCTACCC AACCTCCATG TTGGGTAGCT ACATCAGCCC TTCTCCAGCA
    GAGGCCATGC AAAATAATAC CACCACCATC CAGCCACTTT CTGGAAGTCC TGGGACAAGA
    CACCACACCA TCCTTTCTGA CATGGCGTCC GTTCCCAACT TTCAACACAG ATTAGCAAAT
    CAGGATGGTC CCATACTTGG GCGACAGCAG GCTCCAGCTA ATGGTCATGT GCCTGGAATT
    GCTGGTAGTC CAGTGCAAGG AAGACACACT ATGGTGTCCC AGGGTACACA AAGCAGTCCC
    ATACTGAGCA GACAGTATCC TGTCACCCAG GGGATTCAGA GTAGTCCAAT TCTCAGCAGG
    CAGCCAATGG GACAAGCTGT CCAGAGCAGT CCAGTCCTCA ACCGACAGGC ATCCCTGAAT
    CAAGCAAACC AAAGCAGTCC AGTGTTGAGT AGACAGCAGT CCATTACCCT ACCCAACCAA
    GGCAGCCCGG TCCTAAGCAG ACAGTCCTCG CTTAGTCATC CCAGTCAGGG TAGTCCTGTA
    CTAGGACGCC ACCCTTCCCT AACACAGGGT AGCCCCAGCC TGGATAGGCA TCCGATGTAC
    AGCGGTTACA CCACTCCAGA AGAGCGACAC GGAGCTCTGT CACGCCAAAG CAGCTCTTCA
    GGATACCAGC CGCCATCTAC ACCGTCTTTC CCAGTTTCTC CAGCAGGATA CATCGAGGGA
    GCGGGATTCA GACAGGGGAG TCCTGCTTTC CAACCTCAGT TGCCCGAAAA GAGACGCATG
    TCCAGCAGTG ACCGCACCAA TGGTGGCCTG TCCTATGGGA CGCTCAATGG GAAGATGTCC
    TCACCGGTGT CCAGTGGCGG CAGCACTCCG AGTGTTCATT TCTTCCACAC ACTCCCCGAT
    TTCTCCAAAC TCAACATGTG TGATGGAAGT CCAGAAACCA GAATGAACGT GAAGTTTGTC
    CAGGACACGT CCAAATTCTG GTATAAACCA GATATCTCGA GAGACCAAGC GATTAACATG
    CTGAAGGATC AAGATCCTGG GGCCTTTGTG ATTCGGGACA GTCATTCATT CAGAGGAGCC
    TACGGCTTGG CTATGAAAGT GGCTTCACCA CCTCCAACCA CTCAACCTAC AAAGAAAGCT
    GGAGATGTCA CTAATGAGTT GGTGAGGCAT TTTCTAATTG AGACGAGTCC GAAGGGTGTG
    AGACTGAAGG GCTGCCCCAA CGAGCCGTAC TTTGGCTGCC TGTCTGCGCT GGTCTACCAG
    CACTCCATCA CTCCACTGGC ACTGCCCTGC AAACTAGTCA TACCAACCAG AGATCCTCTG
    GAAGAGTCAC CTGAGATCGC AACACCAACC AACCCGGCGG CAGATATGCT CAAGCAGGGC
    GCAGGGCAGA AGAGTTCAGC TGATGGCCAT GCATGCAATG TGCTGTACAT CAACTCTGTG
    GATATGGAGT CTTTGACTGG ACCTCAAGCC ATCGCCAAAG CCATTTCAGA AACCATGAAC
    GCCAGTCCGG CACCCTCAGC CACCATTGTT CACTTCAAGG TGTCGGCACA AGGCATCACA
    CTCACAGACA ACCAGAGAAA GCTTTTCTTC AGACGTCATT ATCCCATCGG CACAGTCACG
    TTCTGTGACA TTGATCCACA GGAGAGGAAG TGGAACAAAC CAGAGGGTGG CACTGCCAAA
    TTCTTTGGCT TTGTGGCTAG AAAGCAAGGC AGCACGACAG ACAATGTCAG TCACCTGTTT
    GCTGAGATGG ACCCAGATCA GCCGGCCACC GCCATCGTCA ACTTTGTCTC TAAAGTGATG
    ATCAGCTCTC AGAAGCGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005167988.2
    CDS714..6014
    Translation

    Target ORF information:

    RefSeq Version XM_005167931.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005167931.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
    TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG
    CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG
    GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG
    TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA
    CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG
    TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC
    ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC
    GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC
    TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT
    GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG
    GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA
    GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC
    GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG
    GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT
    AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC
    GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC
    ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA
    CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC
    CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT
    ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT
    GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT
    GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT
    TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA
    GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG
    GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT
    CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC
    ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT
    TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA
    GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT
    GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA
    GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT
    CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA
    GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG
    CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG
    TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA
    GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG
    AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC
    TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA
    GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT
    ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA
    CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC
    CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT
    TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT
    TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA
    GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG
    CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA
    CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA
    TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG
    GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC
    ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG
    CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT
    CCAATTGTTG GTGGTCGATC TCCTCCAGGA TTGGCTAAAA CACCACTGTC CTTTCTGGGC
    CTGAAGCCCC ACAACCCTGC TGAGATCCTG CTCAACCAGA CAGGCTCAGA GCCTCGTAGT
    TATGTGGAGT CTGTGGCGAA GGCAGCAGCA GTACCTGCAT CTCCAACATA TGACAGCGAC
    AGCTTAGCTC GTCCTGCCGG ACCGTCCCAT GCTTACAATG CTCCACTTTC CTCCAGCAGC
    CCCATTCAGA ACCCAGATCC AGTTGAAAGC ACATTGGCTG ACAGCGGGGT CGGGATGAAT
    TCTTTATCCC AGGCTGCTGT CGACATTTTC CATTTCCAGC CCTCCGAAAT CCCATACCAA
    CCACCACCAC CCCCCTACCC AACCTCCATG TTGGGTAGCT ACATCAGCCC TTCTCCAGCA
    GAGGCCATGC AAAATAATAC CACCACCATC CAGCCACTTT CTGGAAGTCC TGGGACAAGA
    CACCACACCA TCCTTTCTGA CATGGCGTCC GTTCCCAACT TTCAACACAG ATTAGCAAAT
    CAGGATGGTC CCATACTTGG GCGACAGCAG GCTCCAGCTA ATGGTCATGT GCCTGGAATT
    GCTGGTAGTC CAGTGCAAGG AAGACACACT ATGGTGTCCC AGGGTACACA AAGCAGTCCC
    ATACTGAGCA GACAGTATCC TGTCACCCAG GGGATTCAGA GTAGTCCAAT TCTCAGCAGG
    CAGCCAATGG GACAAGCTGT CCAGAGCAGT CCAGTCCTCA ACCGACAGGC ATCCCTGAAT
    CAAGCAAACC AAAGCAGTCC AGTGTTGAGT AGACAGCAGT CCATTACCCT ACCCAACCAA
    GGCAGCCCGG TCCTAAGCAG ACAGTCCTCG CTTAGTCATC CCAGTCAGGG TAGTCCTGTA
    CTAGGACGCC ACCCTTCCCT AACACAGGGT AGCCCCAGCC TGGATAGGCA TCCGATGTAC
    AGCGGTTACA CCACTCCAGA AGAGCGACAC GGAGCTCTGT CACGCCAAAG CAGCTCTTCA
    GGATACCAGC CGCCATCTAC ACCGTCTTTC CCAGTTTCTC CAGCAGGATA CATCGAGGGA
    GCGGGATTCA GACAGGGGAG TCCTGCTTTC CAACCTCAGT TGCCCGAAAA GAGACGCATG
    TCCAGCAGTG ACCGCACCAA TGGTGGCCTG TCCTATGGGA CGCTCAATGG GAAGATGTCC
    TCACCGGTGT CCAGTGGCGG CAGCACTCCG AGTGTTCATT TCTTCCACAC ACTCCCCGAT
    TTCTCCAAAC TCAACATGTG TGATGGAAGT CCAGAAACCA GAATGAACGT GAAGTTTGTC
    CAGGACACGT CCAAATTCTG GTATAAACCA GATATCTCGA GAGACCAAGC GATTAACATG
    CTGAAGGATC AAGATCCTGG GGCCTTTGTG ATTCGGGACA GTCATTCATT CAGAGGAGCC
    TACGGCTTGG CTATGAAAGT GGCTTCACCA CCTCCAACCA CTCAACCTAC AAAGAAAGCT
    GGAGATGTCA CTAATGAGTT GGTGAGGCAT TTTCTAATTG AGACGAGTCC GAAGGGTGTG
    AGACTGAAGG GCTGCCCCAA CGAGCCGTAC TTTGGCTGCC TGTCTGCGCT GGTCTACCAG
    CACTCCATCA CTCCACTGGC ACTGCCCTGC AAACTAGTCA TACCAACCAG AGATCCTCTG
    GAAGAGTCAC CTGAGATCGC AACACCAACC AACCCGGCGG CAGATATGCT CAAGCAGGGC
    GCAGGGCAGA AGAGTTCAGC TGATGGCCAT GCATGCAATG TGCTGTACAT CAACTCTGTG
    GATATGGAGT CTTTGACTGG ACCTCAAGCC ATCGCCAAAG CCATTTCAGA AACCATGAAC
    GCCAGTCCGG CACCCTCAGC CACCATTGTT CACTTCAAGG TGTCGGCACA AGGCATCACA
    CTCACAGACA ACCAGAGAAA GCTTTTCTTC AGACGTCATT ATCCCATCGG CACAGTCACG
    TTCTGTGACA TTGATCCACA GGAGAGGAAG TGGAACAAAC CAGAGGGTGG CACTGCCAAA
    TTCTTTGGCT TTGTGGCTAG AAAGCAAGGC AGCACGACAG ACAATGTCAG TCACCTGTTT
    GCTGAGATGG ACCCAGATCA GCCGGCCACC GCCATCGTCA ACTTTGTCTC TAAAGTGATG
    ATCAGCTCTC AGAAGCGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60381
    Clone ID Related Accession (Same CDS sequence) XM_017357850.1
    Accession Version XM_017357850.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product tensin-1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCCACCG CTGTGTTTCA GGTCTCGTCC TCCTGTGTTC CTGTCGCCAA CTATGAACTG 
    GCCCCCAGCA ATGAGCTCCC CCTGAAACAT ATCGAGACCA CAGGCTCAAC CAAATCCTCC
    AAGAGCATGG AGTCTCGACG CAGACCGTCA AGGAGTTTGA GTTTGATCCA GGCCATGGAG
    GAGAACTATG AGGTGGATCT GGTTTACATC ACCGAGCGCA TCATTTCTGT CACCTTTCCC
    AGTAATGCAG AGGAGCCCAG TTACAGCGCC AACATTAAAG AAGTGGCAAA CATGCTGCGC
    TCCAAACATG GAGACAACTA CCTGCTCTTC AACCTCAGTG AAAAGCGCTT GGATATCTCC
    AAGCTGAATC CCAAAGTTCT GGATTTTGGC TGGCCGGATC ATCACGCTCC TGCTTTGGAT
    AAGATCTGCA GCATCTGCAA AGCCATGGAC ACTTGGATGA ACGCTGGCAG TCACAACGTG
    GTGGTCATAC ACAACAAGGG AAACCGAGGT CGAACTGGGG TGGTTGTGGC TGCGTACATG
    CATTACAGCA ACATATCAGC GAGTGCCGAC CAGGCTCTGG ACAGGTTTGC CATGAAGAGG
    TTTTATGAAG ATAAAGTGCT TCCTGTGGGT CAGCCTTCCC AGAGAAGATA TGTGCAGTAC
    TTCAGCGGTC TGCTCTCCGG CCACATTAAG ATCAACAACA AACCCCTGTT TCTCCATCAC
    GTCATCATGC ACGGCATCCC AAACTTCGAG TCTAAAGGAG GTTGCCGCCC TTTCCTGAAG
    ATCTACCAGG CCATGCAGCC CGTCTACACA TCTGGCATCT ATAATGTCCA AGGAGACAGT
    CAGACTAGTA TCTGCATCAC CATTGAACCG GGCCTGCTTT TGAAAGGAGA CATACTGCTA
    AAGTGTTATC ACAAGCGTTT CCGCAGCCCA TGCCGTGATG TCATATTCAG AGTTCAGTTT
    CACACCTGTG CTGTTCATGA TCTGGGTATC GTCTTCGGCA AAGATGAGCT TGATGAAACC
    TTTAAAGACG ACAGATTTCC AGAATATGGA AAGGTGGAAT TCGTCTTTTC TTTTGGCCCA
    GAAAAAATCC AAGGAATGGA CCACCTTGAG AACGGGCCCA GTGTTTCTGT GGACTACAAT
    ACCCAAGATG CACTAATCCG CTGGGATTCC TACGAGAACT TCAACCAGCG CTGTGAAGAC
    TCCATGGAAG ACGTTATTCA CACCGAAGGT CCATTGGATG GTAGTCTCTA CGCTAAAGTT
    CGTAAGAAAG AGTCTCTGGA AGGAGGGGTT ACAGCCAATG GTCTCCCAGC TGCTGCTGAT
    CACACTTTAC CTGCTGTAGA CCACACTCTA CCACCAGTTG ACCACGCCTT GTCTGTAAGC
    AGTGATTCAG GCAACTCCAC AGCGTCCATC AAGACTGACC GCACTGATGA AGCCAGCCAT
    CAGCCAGCAT TGGTGAGTCA GCCATCTTCC CAGCCCCTCA GCCCTGAGGA GAAACAGGAG
    CTGGACCAGT TGCTCAGTGG TTTGGAAGCA CCAATGCATC GCACAGGTTA CCAAAGCAGC
    CCTGGTGGGG GTGGTGGTGG AGGTGGTGGA GTCCTACATC TCGTCCCTGC CCAGGTACAC
    GTCAATGGAC ACAGCAACAT AGACAGAGAG ACAGATATCC TTGATGATGA CTTGCCCATC
    AGCCAGGAAG GGAACAGTGT GGACAGTCTT GGAACTCTCT CATCTTTTGA GGGCAGAGCC
    ACTCCTGCGG ATCTATACTA CCAGTCAGAG ACAGTCATTA ATGGACAAGA TATGCCCTAC
    TTAGAGAAGA GTGTCCATGA GAAAATGCTA GAGCCCAGAG TTCACAGCTC ATCATCTGTC
    CAAGAGCACT TGGGAGCCCC TAGTGGGTAT GCTAACCAGA ATGGCAACAT CTATCGATCT
    CAGTCATTAG GAGCAACCCC GAACACAGAC CAGAAGTACA TGCCCAAAGC TCCAGCACGC
    ACAACCAGCA GTAGGGATGC CGTGCAGAGA CTGAATGTTT GGCAACAATT TGGTGTACCA
    GAAGTCCCAG TCACAGATGG GGTCACCTTC AACCCTGTAC TACAATCCCA CAGCCTTCCA
    GAGTTCCCTC AGATGGCATC TCAACAAGAG ATTGAGCAGT CCATTGAAGC CCTCAACATG
    CTGATGCTGG ACCTTGATCC AGCACTTACT CAAGTCCCCA AATCCCACAG CGCTCCATCT
    GGAGAAAGTG GTGTGGTCAC CACCCAACCA TCTTTCTCTC AAACCCAAGC ACGCCCATCC
    TACCAGGCTG ACACTGCTAT TCATGACCGC GGTGCGGCCT CTTCAATGGG ATTTCCTTTG
    ACCTTTCAAC AAGTAACTGG AGAGCCTTCA CAACCCACCA TGAAACAGCA GAAACCCACA
    ACTACATCCC AGCATGTCGA ATTCGCTTCT GAAGCAGGAG GTCAGGCCCC CATGTCCCAG
    TCCACAGTGC CTTCCTCCAC CTTTGGAGTT CTCCAGTTGA AACCCCTCAA CATTTATCCC
    TCAAGTACCA CATCCCAGTC CCCCGAGCCT CAGGAGACTC AGCGTAGCTA CTCTTCTTCC
    TCATCCCCTC TTCCCAAAGA GTCTGAACCA GAAGATAGCA GCTACAAATT GGAGGGTCTT
    GTAGCTCACC GTGTAGCTGG ACTTCCATCT GGTGAAATGT CGCCAGATGA GGCTGCAGTT
    CCTGGACGCT GTCGGACCCT TAGTGAGGGC CTTTCCCAAG ACGAAAGCCC AACTCGAGGA
    AGCTCAGTCC GCTCCCCCAT TCGCTGCATC TCACCAGAGC TGGCCAATAC AATTGCTCTG
    AACCCTGGGG GGCGGCCCAA GGAGAGGCAC ATGCACAGCT ACAGAGAAGC GTTTGAGGAA
    ATGGATGGGG GTCCCGTTAG CCCCCCTCCA ATTGTTGGTG GTGAGGTCCT ACCCCAAACC
    CCTGCCTTCC CTGTCTCGCC ACAAACCCCT TACTTCAACA TGTGTCGATC TCCTCCAGGA
    TTGGCTAAAA CACCACTGTC CTTTCTGGGC CTGAAGCCCC ACAACCCTGC TGAGATCCTG
    CTCAACCAGA CAGGCTCAGA GAGTGAGGAT GAGGAAGAGC CTCGTAGTTA TGTGGAGTCT
    GTGGCGAAGG CAGCAGCAGT ACCTGCATCT CCAACATATG ACAGCGACAG CTTAGCTCGT
    CCTGCCGGAC CGTCCCATGC TTACAATGCT CCACTTTCCT CCAGCAGCCC CATTCAGAAC
    CCAGATCCAG TTGAAAGCAC ATTGGCTGAC AGCGGGGTCG GGATGAATTC TTTATCCCAG
    GCTGCTGTCG ACATTTTCCA TTTCCAGCCC TCCGAAATCC CATACCAACC ACCACCACCC
    CCCTACCCAA CCTCCATGTT GGGTAGCTAC ATCAGCCCTT CTCCAGCAGA GGCCATGCAA
    AATAATACCA CCACCATCCA GCCACTTTCT GGAAGTCCTG GGACAAGACA CCACACCATC
    CTTTCTGACA TGGCGTCCGT TCCCAACTTT CAACACAGAT TAGCAAATCA GGATGGTCCC
    ATACTTGGGC GACAGCAGGC TCCAGCTAAT GGTCATGTGC CTGGAATTGC TGGTAGTCCA
    GTGCAAGGAA GACACACTAT GGTGTCCCAG GGTACACAAA GCAGTCCCAT ACTGAGCAGA
    CAGTATCCTG TCACCCAGGG GATTCAGAGT AGTCCAATTC TCAGCAGGCA GCCAATGGGA
    CAAGCTGTCC AGAGCAGTCC AGTCCTCAAC CGACAGGCAT CCCTGAATCA AGCAAACCAA
    AGCAGTCCAG TGTTGAGTAG ACAGCAGTCC ATTACCCTAC CCAACCAAGG CAGCCCGGTC
    CTAAGCAGAC AGTCCTCGCT TAGTCATCCC AGTCAGGGTA GTCCTGTACT AGGACGCCAC
    CCTTCCCTAA CACAGGGTAG CCCCAGCCTG GATAGGCATC CGATGTACAG CGGTTACACC
    ACTCCAGAAG AGCGACACGG AGCTCTGTCA CGCCAAAGCA GCTCTTCAGG ATACCAGCCG
    CCATCTACAC CGTCTTTCCC AGTTTCTCCA GCAGGATACA TCGAGGGAGC GGGATTCAGA
    CAGGGGAGTC CTGCTTTCCA ACCTCAGTTG CCCGAAAAGA GACGCATGTC CAGCAGTGAC
    CGCACCAATG GTGGCCTGTC CTATGGGACG CTCAATGGGA AGATGTCCTC ACCGGTGTCC
    AGTGGCGGCA GCACTCCGAG TGTTCATTTC TTCCACACAC TCCCCGATTT CTCCAAACTC
    AACATGTGTG ATGGAAGTCC AGAAACCAGA ATGAACGTGA AGTTTGTCCA GGACACGTCC
    AAATTCTGGT ATAAACCAGA TATCTCGAGA GACCAAGCGA TTAACATGCT GAAGGATCAA
    GATCCTGGGG CCTTTGTGAT TCGGGACAGT CATTCATTCA GAGGAGCCTA CGGCTTGGCT
    ATGAAAGTGG CTTCACCACC TCCAACCACT CAACCTACAA AGAAAGCTGG AGATGTCACT
    AATGAGTTGG TGAGGCATTT TCTAATTGAG ACGAGTCCGA AGGGTGTGAG ACTGAAGGGC
    TGCCCCAACG AGCCGTACTT TGGCTGCCTG TCTGCGCTGG TCTACCAGCA CTCCATCACT
    CCACTGGCAC TGCCCTGCAA ACTAGTCATA CCAACCAGAG ATCCTCTGGA AGAGTCACCT
    GAGATCGCAA CACCAACCAA CCCGGCGGCA GATATGCTCA AGCAGGGCGC AGGGCAGAAG
    AGTTCAGCTG ATGGCCATGC ATGCAATGTG CTGTACATCA ACTCTGTGGA TATGGAGTCT
    TTGACTGGAC CTCAAGCCAT CGCCAAAGCC ATTTCAGAAA CCATGAACGC CAGTCCGGCA
    CCCTCAGCCA CCATTGTTCA CTTCAAGGTG TCGGCACAAG GCATCACACT CACAGACAAC
    CAGAGAAAGC TTTTCTTCAG ACGTCATTAT CCCATCGGCA CAGTCACGTT CTGTGACATT
    GATCCACAGG AGAGGAAGTG GAACAAACCA GAGGGTGGCA CTGCCAAATT CTTTGGCTTT
    GTGGCTAGAA AGCAAGGCAG CACGACAGAC AATGTCAGTC ACCTGTTTGC TGAGATGGAC
    CCAGATCAGC CGGCCACCGC CATCGTCAAC TTTGTCTCTA AAGTGATGAT CAGCTCTCAG
    AAGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213339.1
    CDS387..5555
    Translation

    Target ORF information:

    RefSeq Version XM_017357850.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357850.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCCACCG CTGTGTTTCA GGTCTCGTCC TCCTGTGTTC CTGTCGCCAA CTATGAACTG 
    GCCCCCAGCA ATGAGCTCCC CCTGAAACAT ATCGAGACCA CAGGCTCAAC CAAATCCTCC
    AAGAGCATGG AGTCTCGACG CAGACCGTCA AGGAGTTTGA GTTTGATCCA GGCCATGGAG
    GAGAACTATG AGGTGGATCT GGTTTACATC ACCGAGCGCA TCATTTCTGT CACCTTTCCC
    AGTAATGCAG AGGAGCCCAG TTACAGCGCC AACATTAAAG AAGTGGCAAA CATGCTGCGC
    TCCAAACATG GAGACAACTA CCTGCTCTTC AACCTCAGTG AAAAGCGCTT GGATATCTCC
    AAGCTGAATC CCAAAGTTCT GGATTTTGGC TGGCCGGATC ATCACGCTCC TGCTTTGGAT
    AAGATCTGCA GCATCTGCAA AGCCATGGAC ACTTGGATGA ACGCTGGCAG TCACAACGTG
    GTGGTCATAC ACAACAAGGG AAACCGAGGT CGAACTGGGG TGGTTGTGGC TGCGTACATG
    CATTACAGCA ACATATCAGC GAGTGCCGAC CAGGCTCTGG ACAGGTTTGC CATGAAGAGG
    TTTTATGAAG ATAAAGTGCT TCCTGTGGGT CAGCCTTCCC AGAGAAGATA TGTGCAGTAC
    TTCAGCGGTC TGCTCTCCGG CCACATTAAG ATCAACAACA AACCCCTGTT TCTCCATCAC
    GTCATCATGC ACGGCATCCC AAACTTCGAG TCTAAAGGAG GTTGCCGCCC TTTCCTGAAG
    ATCTACCAGG CCATGCAGCC CGTCTACACA TCTGGCATCT ATAATGTCCA AGGAGACAGT
    CAGACTAGTA TCTGCATCAC CATTGAACCG GGCCTGCTTT TGAAAGGAGA CATACTGCTA
    AAGTGTTATC ACAAGCGTTT CCGCAGCCCA TGCCGTGATG TCATATTCAG AGTTCAGTTT
    CACACCTGTG CTGTTCATGA TCTGGGTATC GTCTTCGGCA AAGATGAGCT TGATGAAACC
    TTTAAAGACG ACAGATTTCC AGAATATGGA AAGGTGGAAT TCGTCTTTTC TTTTGGCCCA
    GAAAAAATCC AAGGAATGGA CCACCTTGAG AACGGGCCCA GTGTTTCTGT GGACTACAAT
    ACCCAAGATG CACTAATCCG CTGGGATTCC TACGAGAACT TCAACCAGCG CTGTGAAGAC
    TCCATGGAAG ACGTTATTCA CACCGAAGGT CCATTGGATG GTAGTCTCTA CGCTAAAGTT
    CGTAAGAAAG AGTCTCTGGA AGGAGGGGTT ACAGCCAATG GTCTCCCAGC TGCTGCTGAT
    CACACTTTAC CTGCTGTAGA CCACACTCTA CCACCAGTTG ACCACGCCTT GTCTGTAAGC
    AGTGATTCAG GCAACTCCAC AGCGTCCATC AAGACTGACC GCACTGATGA AGCCAGCCAT
    CAGCCAGCAT TGGTGAGTCA GCCATCTTCC CAGCCCCTCA GCCCTGAGGA GAAACAGGAG
    CTGGACCAGT TGCTCAGTGG TTTGGAAGCA CCAATGCATC GCACAGGTTA CCAAAGCAGC
    CCTGGTGGGG GTGGTGGTGG AGGTGGTGGA GTCCTACATC TCGTCCCTGC CCAGGTACAC
    GTCAATGGAC ACAGCAACAT AGACAGAGAG ACAGATATCC TTGATGATGA CTTGCCCATC
    AGCCAGGAAG GGAACAGTGT GGACAGTCTT GGAACTCTCT CATCTTTTGA GGGCAGAGCC
    ACTCCTGCGG ATCTATACTA CCAGTCAGAG ACAGTCATTA ATGGACAAGA TATGCCCTAC
    TTAGAGAAGA GTGTCCATGA GAAAATGCTA GAGCCCAGAG TTCACAGCTC ATCATCTGTC
    CAAGAGCACT TGGGAGCCCC TAGTGGGTAT GCTAACCAGA ATGGCAACAT CTATCGATCT
    CAGTCATTAG GAGCAACCCC GAACACAGAC CAGAAGTACA TGCCCAAAGC TCCAGCACGC
    ACAACCAGCA GTAGGGATGC CGTGCAGAGA CTGAATGTTT GGCAACAATT TGGTGTACCA
    GAAGTCCCAG TCACAGATGG GGTCACCTTC AACCCTGTAC TACAATCCCA CAGCCTTCCA
    GAGTTCCCTC AGATGGCATC TCAACAAGAG ATTGAGCAGT CCATTGAAGC CCTCAACATG
    CTGATGCTGG ACCTTGATCC AGCACTTACT CAAGTCCCCA AATCCCACAG CGCTCCATCT
    GGAGAAAGTG GTGTGGTCAC CACCCAACCA TCTTTCTCTC AAACCCAAGC ACGCCCATCC
    TACCAGGCTG ACACTGCTAT TCATGACCGC GGTGCGGCCT CTTCAATGGG ATTTCCTTTG
    ACCTTTCAAC AAGTAACTGG AGAGCCTTCA CAACCCACCA TGAAACAGCA GAAACCCACA
    ACTACATCCC AGCATGTCGA ATTCGCTTCT GAAGCAGGAG GTCAGGCCCC CATGTCCCAG
    TCCACAGTGC CTTCCTCCAC CTTTGGAGTT CTCCAGTTGA AACCCCTCAA CATTTATCCC
    TCAAGTACCA CATCCCAGTC CCCCGAGCCT CAGGAGACTC AGCGTAGCTA CTCTTCTTCC
    TCATCCCCTC TTCCCAAAGA GTCTGAACCA GAAGATAGCA GCTACAAATT GGAGGGTCTT
    GTAGCTCACC GTGTAGCTGG ACTTCCATCT GGTGAAATGT CGCCAGATGA GGCTGCAGTT
    CCTGGACGCT GTCGGACCCT TAGTGAGGGC CTTTCCCAAG ACGAAAGCCC AACTCGAGGA
    AGCTCAGTCC GCTCCCCCAT TCGCTGCATC TCACCAGAGC TGGCCAATAC AATTGCTCTG
    AACCCTGGGG GGCGGCCCAA GGAGAGGCAC ATGCACAGCT ACAGAGAAGC GTTTGAGGAA
    ATGGATGGGG GTCCCGTTAG CCCCCCTCCA ATTGTTGGTG GTGAGGTCCT ACCCCAAACC
    CCTGCCTTCC CTGTCTCGCC ACAAACCCCT TACTTCAACA TGTGTCGATC TCCTCCAGGA
    TTGGCTAAAA CACCACTGTC CTTTCTGGGC CTGAAGCCCC ACAACCCTGC TGAGATCCTG
    CTCAACCAGA CAGGCTCAGA GAGTGAGGAT GAGGAAGAGC CTCGTAGTTA TGTGGAGTCT
    GTGGCGAAGG CAGCAGCAGT ACCTGCATCT CCAACATATG ACAGCGACAG CTTAGCTCGT
    CCTGCCGGAC CGTCCCATGC TTACAATGCT CCACTTTCCT CCAGCAGCCC CATTCAGAAC
    CCAGATCCAG TTGAAAGCAC ATTGGCTGAC AGCGGGGTCG GGATGAATTC TTTATCCCAG
    GCTGCTGTCG ACATTTTCCA TTTCCAGCCC TCCGAAATCC CATACCAACC ACCACCACCC
    CCCTACCCAA CCTCCATGTT GGGTAGCTAC ATCAGCCCTT CTCCAGCAGA GGCCATGCAA
    AATAATACCA CCACCATCCA GCCACTTTCT GGAAGTCCTG GGACAAGACA CCACACCATC
    CTTTCTGACA TGGCGTCCGT TCCCAACTTT CAACACAGAT TAGCAAATCA GGATGGTCCC
    ATACTTGGGC GACAGCAGGC TCCAGCTAAT GGTCATGTGC CTGGAATTGC TGGTAGTCCA
    GTGCAAGGAA GACACACTAT GGTGTCCCAG GGTACACAAA GCAGTCCCAT ACTGAGCAGA
    CAGTATCCTG TCACCCAGGG GATTCAGAGT AGTCCAATTC TCAGCAGGCA GCCAATGGGA
    CAAGCTGTCC AGAGCAGTCC AGTCCTCAAC CGACAGGCAT CCCTGAATCA AGCAAACCAA
    AGCAGTCCAG TGTTGAGTAG ACAGCAGTCC ATTACCCTAC CCAACCAAGG CAGCCCGGTC
    CTAAGCAGAC AGTCCTCGCT TAGTCATCCC AGTCAGGGTA GTCCTGTACT AGGACGCCAC
    CCTTCCCTAA CACAGGGTAG CCCCAGCCTG GATAGGCATC CGATGTACAG CGGTTACACC
    ACTCCAGAAG AGCGACACGG AGCTCTGTCA CGCCAAAGCA GCTCTTCAGG ATACCAGCCG
    CCATCTACAC CGTCTTTCCC AGTTTCTCCA GCAGGATACA TCGAGGGAGC GGGATTCAGA
    CAGGGGAGTC CTGCTTTCCA ACCTCAGTTG CCCGAAAAGA GACGCATGTC CAGCAGTGAC
    CGCACCAATG GTGGCCTGTC CTATGGGACG CTCAATGGGA AGATGTCCTC ACCGGTGTCC
    AGTGGCGGCA GCACTCCGAG TGTTCATTTC TTCCACACAC TCCCCGATTT CTCCAAACTC
    AACATGTGTG ATGGAAGTCC AGAAACCAGA ATGAACGTGA AGTTTGTCCA GGACACGTCC
    AAATTCTGGT ATAAACCAGA TATCTCGAGA GACCAAGCGA TTAACATGCT GAAGGATCAA
    GATCCTGGGG CCTTTGTGAT TCGGGACAGT CATTCATTCA GAGGAGCCTA CGGCTTGGCT
    ATGAAAGTGG CTTCACCACC TCCAACCACT CAACCTACAA AGAAAGCTGG AGATGTCACT
    AATGAGTTGG TGAGGCATTT TCTAATTGAG ACGAGTCCGA AGGGTGTGAG ACTGAAGGGC
    TGCCCCAACG AGCCGTACTT TGGCTGCCTG TCTGCGCTGG TCTACCAGCA CTCCATCACT
    CCACTGGCAC TGCCCTGCAA ACTAGTCATA CCAACCAGAG ATCCTCTGGA AGAGTCACCT
    GAGATCGCAA CACCAACCAA CCCGGCGGCA GATATGCTCA AGCAGGGCGC AGGGCAGAAG
    AGTTCAGCTG ATGGCCATGC ATGCAATGTG CTGTACATCA ACTCTGTGGA TATGGAGTCT
    TTGACTGGAC CTCAAGCCAT CGCCAAAGCC ATTTCAGAAA CCATGAACGC CAGTCCGGCA
    CCCTCAGCCA CCATTGTTCA CTTCAAGGTG TCGGCACAAG GCATCACACT CACAGACAAC
    CAGAGAAAGC TTTTCTTCAG ACGTCATTAT CCCATCGGCA CAGTCACGTT CTGTGACATT
    GATCCACAGG AGAGGAAGTG GAACAAACCA GAGGGTGGCA CTGCCAAATT CTTTGGCTTT
    GTGGCTAGAA AGCAAGGCAG CACGACAGAC AATGTCAGTC ACCTGTTTGC TGAGATGGAC
    CCAGATCAGC CGGCCACCGC CATCGTCAAC TTTGTCTCTA AAGTGATGAT CAGCTCTCAG
    AAGCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42135
    Clone ID Related Accession (Same CDS sequence) XM_009304966.2
    Accession Version XM_009304966.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product tensin-1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGTCTC GACGCAGACC GTCAAGGAGT TTGAGTTTGA TCCAGGCCAT GGAGGAGAAC 
    TATGAGGTGG ATCTGGTTTA CATCACCGAG CGCATCATTT CTGTCACCTT TCCCAGTAAT
    GCAGAGGAGC CCAGTTACAG CGCCAACATT AAAGAAGTGG CAAACATGCT GCGCTCCAAA
    CATGGAGACA ACTACCTGCT CTTCAACCTC AGTGAAAAGC GCTTGGATAT CTCCAAGCTG
    AATCCCAAAG TTCTGGATTT TGGCTGGCCG GATCATCACG CTCCTGCTTT GGATAAGATC
    TGCAGCATCT GCAAAGCCAT GGACACTTGG ATGAACGCTG GCAGTCACAA CGTGGTGGTC
    ATACACAACA AGGGAAACCG AGGTCGAACT GGGGTGGTTG TGGCTGCGTA CATGCATTAC
    AGCAACATAT CAGCGAGTGC CGACCAGGCT CTGGACAGGT TTGCCATGAA GAGGTTTTAT
    GAAGATAAAG TGCTTCCTGT GGGTCAGCCT TCCCAGAGAA GATATGTGCA GTACTTCAGC
    GGTCTGCTCT CCGGCCACAT TAAGATCAAC AACAAACCCC TGTTTCTCCA TCACGTCATC
    ATGCACGGCA TCCCAAACTT CGAGTCTAAA GGAGGTTGCC GCCCTTTCCT GAAGATCTAC
    CAGGCCATGC AGCCCGTCTA CACATCTGGC ATCTATAATG TCCAAGGAGA CAGTCAGACT
    AGTATCTGCA TCACCATTGA ACCGGGCCTG CTTTTGAAAG GAGACATACT GCTAAAGTGT
    TATCACAAGC GTTTCCGCAG CCCATGCCGT GATGTCATAT TCAGAGTTCA GTTTCACACC
    TGTGCTGTTC ATGATCTGGG TATCGTCTTC GGCAAAGATG AGCTTGATGA AACCTTTAAA
    GACGACAGAT TTCCAGAATA TGGAAAGGTG GAATTCGTCT TTTCTTTTGG CCCAGAAAAA
    ATCCAAGGAA TGGACCACCT TGAGAACGGG CCCAGTGTTT CTGTGGACTA CAATACCCAA
    GATGCACTAA TCCGCTGGGA TTCCTACGAG AACTTCAACC AGCGCTGTGA AGACTCCATG
    GAAGACGTTA TTCACACCGA AGGTCCATTG GATGGTAGTC TCTACGCTAA AGTTCGTAAG
    AAAGAGTCTC TGGAAGGAGG GGTTACAGCC AATGGTCTCC CAGCTGCTGC TGATCACACT
    TTACCTGCTG TAGACCACAC TCTACCACCA GTTGACCACG CCTTGTCTGT AAGCAGTGAT
    TCAGGCAACT CCACAGCGTC CATCAAGACT GACCGCACTG ATGAAGCCAG CCATCAGCCA
    GCATTGGTGA GTCAGCCATC TTCCCAGCCC CTCAGCCCTG AGGAGAAACA GGAGCTGGAC
    CAGTTGCTCA GTGGTTTGGA AGCACCAATG CATCGCACAG GTTACCAAAG CAGCCCTGGT
    GGGGGTGGTG GTGGAGGTGG TGGAGTCCTA CATCTCGTCC CTGCCCAGGT ACACGTCAAT
    GGACACAGCA ACATAGACAG AGAGACAGAT ATCCTTGATG ATGACTTGCC CATCAGCCAG
    GAAGGGAACA GTGTGGACAG TCTTGGAACT CTCTCATCTT TTGAGGGCAG AGCCACTCCT
    GCGGATCTAT ACTACCAGTC AGAGACAGTC ATTAATGGAC AAGATATGCC CTACTTAGAG
    AAGAGTGTCC ATGAGAAAAT GCTAGAGCCC AGAGTTCACA GCTCATCATC TGTCCAAGAG
    CACTTGGGAG CCCCTAGTGG GTATGCTAAC CAGAATGGCA ACATCTATCG ATCTCAGTCA
    TTAGGAGCAA CCCCGAACAC AGACCAGAAG TACATGCCCA AAGCTCCAGC ACGCACAACC
    AGCAGTAGGG ATGCCGTGCA GAGACTGAAT GTTTGGCAAC AATTTGGTGT ACCAGAAGTC
    CCAGTCACAG ATGGGGTCAC CTTCAACCCT GTACTACAAT CCCACAGCCT TCCAGAGTTC
    CCTCAGATGG CATCTCAACA AGAGATTGAG CAGTCCATTG AAGCCCTCAA CATGCTGATG
    CTGGACCTTG ATCCAGCACT TACTCAAGTC CCCAAATCCC ACAGCGCTCC ATCTGGAGAA
    AGTGGTGTGG TCACCACCCA ACCATCTTTC TCTCAAACCC AAGCACGCCC ATCCTACCAG
    GCTGACACTG CTATTCATGA CCGCGGTGCG GCCTCTTCAA TGGGATTTCC TTTGACCTTT
    CAACAAGTAA CTGGAGAGCC TTCACAACCC ACCATGAAAC AGCAGAAACC CACAACTACA
    TCCCAGCATG TCGAATTCGC TTCTGAAGCA GGAGGTCAGG CCCCCATGTC CCAGTCCACA
    GTGCCTTCCT CCACCTTTGG AGTTCTCCAG TTGAAACCCC TCAACATTTA TCCCTCAAGT
    ACCACATCCC AGTCCCCCGA GCCTCAGGAG ACTCAGCGTA GCTACTCTTC TTCCTCATCC
    CCTCTTCCCA AAGAGTCTGA ACCAGAAGAT AGCAGCTACA AATTGGAGGG TCTTGTAGCT
    CACCGTGTAG CTGGACTTCC ATCTGGTGAA ATGTCGCCAG ATGAGGCTGC AGTTCCTGGA
    CGCTGTCGGA CCCTTAGTGA GGGCCTTTCC CAAGACGAAA GCCCAACTCG AGGAAGCTCA
    GTCCGCTCCC CCATTCGCTG CATCTCACCA GAGCTGGCCA ATACAATTGC TCTGAACCCT
    GGGGGGCGGC CCAAGGAGAG GCACATGCAC AGCTACAGAG AAGCGTTTGA GGAAATGGAT
    GGGGGTCCCG TTAGCCCCCC TCCAATTGTT GGTGGTGAGG TCCTACCCCA AACCCCTGCC
    TTCCCTGTCT CGCCACAAAC CCCTTACTTC AACATGTGTC GATCTCCTCC AGGATTGGCT
    AAAACACCAC TGTCCTTTCT GGGCCTGAAG CCCCACAACC CTGCTGAGAT CCTGCTCAAC
    CAGACAGGCT CAGAGAGTGA GGATGAGGAA GAGCCTCGTA GTTATGTGGA GTCTGTGGCG
    AAGGCAGCAG CAGTACCTGC ATCTCCAACA TATGACAGCG ACAGCTTAGC TCGTCCTGCC
    GGACCGTCCC ATGCTTACAA TGCTCCACTT TCCTCCAGCA GCCCCATTCA GAACCCAGAT
    CCAGTTGAAA GCACATTGGC TGACAGCGGG GTCGGGATGA ATTCTTTATC CCAGGCTGCT
    GTCGACATTT TCCATTTCCA GCCCTCCGAA ATCCCATACC AACCACCACC ACCCCCCTAC
    CCAACCTCCA TGTTGGGTAG CTACATCAGC CCTTCTCCAG CAGAGGCCAT GCAAAATAAT
    ACCACCACCA TCCAGCCACT TTCTGGAAGT CCTGGGACAA GACACCACAC CATCCTTTCT
    GACATGGCGT CCGTTCCCAA CTTTCAACAC AGATTAGCAA ATCAGGATGG TCCCATACTT
    GGGCGACAGC AGGCTCCAGC TAATGGTCAT GTGCCTGGAA TTGCTGGTAG TCCAGTGCAA
    GGAAGACACA CTATGGTGTC CCAGGGTACA CAAAGCAGTC CCATACTGAG CAGACAGTAT
    CCTGTCACCC AGGGGATTCA GAGTAGTCCA ATTCTCAGCA GGCAGCCAAT GGGACAAGCT
    GTCCAGAGCA GTCCAGTCCT CAACCGACAG GCATCCCTGA ATCAAGCAAA CCAAAGCAGT
    CCAGTGTTGA GTAGACAGCA GTCCATTACC CTACCCAACC AAGGCAGCCC GGTCCTAAGC
    AGACAGTCCT CGCTTAGTCA TCCCAGTCAG GGTAGTCCTG TACTAGGACG CCACCCTTCC
    CTAACACAGG GTAGCCCCAG CCTGGATAGG CATCCGATGT ACAGCGGTTA CACCACTCCA
    GAAGAGCGAC ACGGAGCTCT GTCACGCCAA AGCAGCTCTT CAGGATACCA GCCGCCATCT
    ACACCGTCTT TCCCAGTTTC TCCAGCAGGA TACATCGAGG GAGCGGGATT CAGACAGGGG
    AGTCCTGCTT TCCAACCTCA GTTGCCCGAA AAGAGACGCA TGTCCAGCAG TGACCGCACC
    AATGGTGGCC TGTCCTATGG GACGCTCAAT GGGAAGATGT CCTCACCGGT GTCCAGTGGC
    GGCAGCACTC CGAGTGTTCA TTTCTTCCAC ACACTCCCCG ATTTCTCCAA ACTCAACATG
    TGTGATGGAA GTCCAGAAAC CAGAATGAAC GTGAAGTTTG TCCAGGACAC GTCCAAATTC
    TGGTATAAAC CAGATATCTC GAGAGACCAA GCGATTAACA TGCTGAAGGA TCAAGATCCT
    GGGGCCTTTG TGATTCGGGA CAGTCATTCA TTCAGAGGAG CCTACGGCTT GGCTATGAAA
    GTGGCTTCAC CACCTCCAAC CACTCAACCT ACAAAGAAAG CTGGAGATGT CACTAATGAG
    TTGGTGAGGC ATTTTCTAAT TGAGACGAGT CCGAAGGGTG TGAGACTGAA GGGCTGCCCC
    AACGAGCCGT ACTTTGGCTG CCTGTCTGCG CTGGTCTACC AGCACTCCAT CACTCCACTG
    GCACTGCCCT GCAAACTAGT CATACCAACC AGAGATCCTC TGGAAGAGTC ACCTGAGATC
    GCAACACCAA CCAACCCGGC GGCAGATATG CTCAAGCAGG GCGCAGGGCA GAAGAGTTCA
    GCTGATGGCC ATGCATGCAA TGTGCTGTAC ATCAACTCTG TGGATATGGA GTCTTTGACT
    GGACCTCAAG CCATCGCCAA AGCCATTTCA GAAACCATGA ACGCCAGTCC GGCACCCTCA
    GCCACCATTG TTCACTTCAA GGTGTCGGCA CAAGGCATCA CACTCACAGA CAACCAGAGA
    AAGCTTTTCT TCAGACGTCA TTATCCCATC GGCACAGTCA CGTTCTGTGA CATTGATCCA
    CAGGAGAGGA AGTGGAACAA ACCAGAGGGT GGCACTGCCA AATTCTTTGG CTTTGTGGCT
    AGAAAGCAAG GCAGCACGAC AGACAATGTC AGTCACCTGT TTGCTGAGAT GGACCCAGAT
    CAGCCGGCCA CCGCCATCGT CAACTTTGTC TCTAAAGTGA TGATCAGCTC TCAGAAGCGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303241.1
    CDS1839..6881
    Translation

    Target ORF information:

    RefSeq Version XM_009304966.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tensin 1b (tns1b), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304966.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGTCTC GACGCAGACC GTCAAGGAGT TTGAGTTTGA TCCAGGCCAT GGAGGAGAAC 
    TATGAGGTGG ATCTGGTTTA CATCACCGAG CGCATCATTT CTGTCACCTT TCCCAGTAAT
    GCAGAGGAGC CCAGTTACAG CGCCAACATT AAAGAAGTGG CAAACATGCT GCGCTCCAAA
    CATGGAGACA ACTACCTGCT CTTCAACCTC AGTGAAAAGC GCTTGGATAT CTCCAAGCTG
    AATCCCAAAG TTCTGGATTT TGGCTGGCCG GATCATCACG CTCCTGCTTT GGATAAGATC
    TGCAGCATCT GCAAAGCCAT GGACACTTGG ATGAACGCTG GCAGTCACAA CGTGGTGGTC
    ATACACAACA AGGGAAACCG AGGTCGAACT GGGGTGGTTG TGGCTGCGTA CATGCATTAC
    AGCAACATAT CAGCGAGTGC CGACCAGGCT CTGGACAGGT TTGCCATGAA GAGGTTTTAT
    GAAGATAAAG TGCTTCCTGT GGGTCAGCCT TCCCAGAGAA GATATGTGCA GTACTTCAGC
    GGTCTGCTCT CCGGCCACAT TAAGATCAAC AACAAACCCC TGTTTCTCCA TCACGTCATC
    ATGCACGGCA TCCCAAACTT CGAGTCTAAA GGAGGTTGCC GCCCTTTCCT GAAGATCTAC
    CAGGCCATGC AGCCCGTCTA CACATCTGGC ATCTATAATG TCCAAGGAGA CAGTCAGACT
    AGTATCTGCA TCACCATTGA ACCGGGCCTG CTTTTGAAAG GAGACATACT GCTAAAGTGT
    TATCACAAGC GTTTCCGCAG CCCATGCCGT GATGTCATAT TCAGAGTTCA GTTTCACACC
    TGTGCTGTTC ATGATCTGGG TATCGTCTTC GGCAAAGATG AGCTTGATGA AACCTTTAAA
    GACGACAGAT TTCCAGAATA TGGAAAGGTG GAATTCGTCT TTTCTTTTGG CCCAGAAAAA
    ATCCAAGGAA TGGACCACCT TGAGAACGGG CCCAGTGTTT CTGTGGACTA CAATACCCAA
    GATGCACTAA TCCGCTGGGA TTCCTACGAG AACTTCAACC AGCGCTGTGA AGACTCCATG
    GAAGACGTTA TTCACACCGA AGGTCCATTG GATGGTAGTC TCTACGCTAA AGTTCGTAAG
    AAAGAGTCTC TGGAAGGAGG GGTTACAGCC AATGGTCTCC CAGCTGCTGC TGATCACACT
    TTACCTGCTG TAGACCACAC TCTACCACCA GTTGACCACG CCTTGTCTGT AAGCAGTGAT
    TCAGGCAACT CCACAGCGTC CATCAAGACT GACCGCACTG ATGAAGCCAG CCATCAGCCA
    GCATTGGTGA GTCAGCCATC TTCCCAGCCC CTCAGCCCTG AGGAGAAACA GGAGCTGGAC
    CAGTTGCTCA GTGGTTTGGA AGCACCAATG CATCGCACAG GTTACCAAAG CAGCCCTGGT
    GGGGGTGGTG GTGGAGGTGG TGGAGTCCTA CATCTCGTCC CTGCCCAGGT ACACGTCAAT
    GGACACAGCA ACATAGACAG AGAGACAGAT ATCCTTGATG ATGACTTGCC CATCAGCCAG
    GAAGGGAACA GTGTGGACAG TCTTGGAACT CTCTCATCTT TTGAGGGCAG AGCCACTCCT
    GCGGATCTAT ACTACCAGTC AGAGACAGTC ATTAATGGAC AAGATATGCC CTACTTAGAG
    AAGAGTGTCC ATGAGAAAAT GCTAGAGCCC AGAGTTCACA GCTCATCATC TGTCCAAGAG
    CACTTGGGAG CCCCTAGTGG GTATGCTAAC CAGAATGGCA ACATCTATCG ATCTCAGTCA
    TTAGGAGCAA CCCCGAACAC AGACCAGAAG TACATGCCCA AAGCTCCAGC ACGCACAACC
    AGCAGTAGGG ATGCCGTGCA GAGACTGAAT GTTTGGCAAC AATTTGGTGT ACCAGAAGTC
    CCAGTCACAG ATGGGGTCAC CTTCAACCCT GTACTACAAT CCCACAGCCT TCCAGAGTTC
    CCTCAGATGG CATCTCAACA AGAGATTGAG CAGTCCATTG AAGCCCTCAA CATGCTGATG
    CTGGACCTTG ATCCAGCACT TACTCAAGTC CCCAAATCCC ACAGCGCTCC ATCTGGAGAA
    AGTGGTGTGG TCACCACCCA ACCATCTTTC TCTCAAACCC AAGCACGCCC ATCCTACCAG
    GCTGACACTG CTATTCATGA CCGCGGTGCG GCCTCTTCAA TGGGATTTCC TTTGACCTTT
    CAACAAGTAA CTGGAGAGCC TTCACAACCC ACCATGAAAC AGCAGAAACC CACAACTACA
    TCCCAGCATG TCGAATTCGC TTCTGAAGCA GGAGGTCAGG CCCCCATGTC CCAGTCCACA
    GTGCCTTCCT CCACCTTTGG AGTTCTCCAG TTGAAACCCC TCAACATTTA TCCCTCAAGT
    ACCACATCCC AGTCCCCCGA GCCTCAGGAG ACTCAGCGTA GCTACTCTTC TTCCTCATCC
    CCTCTTCCCA AAGAGTCTGA ACCAGAAGAT AGCAGCTACA AATTGGAGGG TCTTGTAGCT
    CACCGTGTAG CTGGACTTCC ATCTGGTGAA ATGTCGCCAG ATGAGGCTGC AGTTCCTGGA
    CGCTGTCGGA CCCTTAGTGA GGGCCTTTCC CAAGACGAAA GCCCAACTCG AGGAAGCTCA
    GTCCGCTCCC CCATTCGCTG CATCTCACCA GAGCTGGCCA ATACAATTGC TCTGAACCCT
    GGGGGGCGGC CCAAGGAGAG GCACATGCAC AGCTACAGAG AAGCGTTTGA GGAAATGGAT
    GGGGGTCCCG TTAGCCCCCC TCCAATTGTT GGTGGTGAGG TCCTACCCCA AACCCCTGCC
    TTCCCTGTCT CGCCACAAAC CCCTTACTTC AACATGTGTC GATCTCCTCC AGGATTGGCT
    AAAACACCAC TGTCCTTTCT GGGCCTGAAG CCCCACAACC CTGCTGAGAT CCTGCTCAAC
    CAGACAGGCT CAGAGAGTGA GGATGAGGAA GAGCCTCGTA GTTATGTGGA GTCTGTGGCG
    AAGGCAGCAG CAGTACCTGC ATCTCCAACA TATGACAGCG ACAGCTTAGC TCGTCCTGCC
    GGACCGTCCC ATGCTTACAA TGCTCCACTT TCCTCCAGCA GCCCCATTCA GAACCCAGAT
    CCAGTTGAAA GCACATTGGC TGACAGCGGG GTCGGGATGA ATTCTTTATC CCAGGCTGCT
    GTCGACATTT TCCATTTCCA GCCCTCCGAA ATCCCATACC AACCACCACC ACCCCCCTAC
    CCAACCTCCA TGTTGGGTAG CTACATCAGC CCTTCTCCAG CAGAGGCCAT GCAAAATAAT
    ACCACCACCA TCCAGCCACT TTCTGGAAGT CCTGGGACAA GACACCACAC CATCCTTTCT
    GACATGGCGT CCGTTCCCAA CTTTCAACAC AGATTAGCAA ATCAGGATGG TCCCATACTT
    GGGCGACAGC AGGCTCCAGC TAATGGTCAT GTGCCTGGAA TTGCTGGTAG TCCAGTGCAA
    GGAAGACACA CTATGGTGTC CCAGGGTACA CAAAGCAGTC CCATACTGAG CAGACAGTAT
    CCTGTCACCC AGGGGATTCA GAGTAGTCCA ATTCTCAGCA GGCAGCCAAT GGGACAAGCT
    GTCCAGAGCA GTCCAGTCCT CAACCGACAG GCATCCCTGA ATCAAGCAAA CCAAAGCAGT
    CCAGTGTTGA GTAGACAGCA GTCCATTACC CTACCCAACC AAGGCAGCCC GGTCCTAAGC
    AGACAGTCCT CGCTTAGTCA TCCCAGTCAG GGTAGTCCTG TACTAGGACG CCACCCTTCC
    CTAACACAGG GTAGCCCCAG CCTGGATAGG CATCCGATGT ACAGCGGTTA CACCACTCCA
    GAAGAGCGAC ACGGAGCTCT GTCACGCCAA AGCAGCTCTT CAGGATACCA GCCGCCATCT
    ACACCGTCTT TCCCAGTTTC TCCAGCAGGA TACATCGAGG GAGCGGGATT CAGACAGGGG
    AGTCCTGCTT TCCAACCTCA GTTGCCCGAA AAGAGACGCA TGTCCAGCAG TGACCGCACC
    AATGGTGGCC TGTCCTATGG GACGCTCAAT GGGAAGATGT CCTCACCGGT GTCCAGTGGC
    GGCAGCACTC CGAGTGTTCA TTTCTTCCAC ACACTCCCCG ATTTCTCCAA ACTCAACATG
    TGTGATGGAA GTCCAGAAAC CAGAATGAAC GTGAAGTTTG TCCAGGACAC GTCCAAATTC
    TGGTATAAAC CAGATATCTC GAGAGACCAA GCGATTAACA TGCTGAAGGA TCAAGATCCT
    GGGGCCTTTG TGATTCGGGA CAGTCATTCA TTCAGAGGAG CCTACGGCTT GGCTATGAAA
    GTGGCTTCAC CACCTCCAAC CACTCAACCT ACAAAGAAAG CTGGAGATGT CACTAATGAG
    TTGGTGAGGC ATTTTCTAAT TGAGACGAGT CCGAAGGGTG TGAGACTGAA GGGCTGCCCC
    AACGAGCCGT ACTTTGGCTG CCTGTCTGCG CTGGTCTACC AGCACTCCAT CACTCCACTG
    GCACTGCCCT GCAAACTAGT CATACCAACC AGAGATCCTC TGGAAGAGTC ACCTGAGATC
    GCAACACCAA CCAACCCGGC GGCAGATATG CTCAAGCAGG GCGCAGGGCA GAAGAGTTCA
    GCTGATGGCC ATGCATGCAA TGTGCTGTAC ATCAACTCTG TGGATATGGA GTCTTTGACT
    GGACCTCAAG CCATCGCCAA AGCCATTTCA GAAACCATGA ACGCCAGTCC GGCACCCTCA
    GCCACCATTG TTCACTTCAA GGTGTCGGCA CAAGGCATCA CACTCACAGA CAACCAGAGA
    AAGCTTTTCT TCAGACGTCA TTATCCCATC GGCACAGTCA CGTTCTGTGA CATTGATCCA
    CAGGAGAGGA AGTGGAACAA ACCAGAGGGT GGCACTGCCA AATTCTTTGG CTTTGTGGCT
    AGAAAGCAAG GCAGCACGAC AGACAATGTC AGTCACCTGT TTGCTGAGAT GGACCCAGAT
    CAGCCGGCCA CCGCCATCGT CAACTTTGTC TCTAAAGTGA TGATCAGCTC TCAGAAGCGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.