gapvd1 cDNA ORF clone, Danio rerio(zebrafish)

The following gapvd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gapvd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa30537 NM_001002420.2
Latest version!
Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa14363 XM_005167157.4
Latest version!
Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa41043 XM_009304093.3
Latest version!
Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa41044 XM_009304094.3
Latest version!
Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa41045 XM_009304095.3
Latest version!
Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa41046 XM_009304096.3
Latest version!
Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
ODa41046 XM_009304096.2 Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa41044 XM_009304094.2 Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa14363 XM_005167157.3 Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa41043 XM_009304093.2 Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa41045 XM_009304095.2 Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa30537
    Clone ID Related Accession (Same CDS sequence) NM_001002420.2
    Accession Version NM_001002420.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product GTPase-activating protein and VPS9 domain-containing protein 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX005399.30 and BX511073.3. On Jan 20, 2011 this sequence version replaced NM_001002420.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGCTCTC GCAGCCGAAG CCGATCCAAC ATGGCCCAGG AAGGAGAGAC TGAAGCCAGT
    TCTCAAGAGT CTCTGCAAGA GGTCCCCGAG GAAGTGCTGG TCATCTCTCT AGGAACATCA
    CCACAGACCA TTCCCGGGAT GATGTCAGAA AACGAGGTTC TGACTTTGCA TCTTGGAGAT
    GGAGGACAAG GTGACGCCCC TGTCGACGAC ACCAAATTAC ATGGCAAGCC TGACAAAACA
    CTGCGCTTCT CACTTTGCAG TGACAACCTG GAGGGCATTT CTGAGGGGCC GTCTAACCGC
    TCAAATTCAG TTTCTTCTTT GGATTTGGAA GTGGAGTCCG TGTCAGAAGG AGGACCAGGA
    CCGTCTGGTA GTAACGGTGC AGAAGCCTTA CAGCTACTGG AGCACGAACA AGCCACTACA
    CAGGATAATT TGGACGATAA ACTGCGCAAG TTTGAGATTC GGGACATGAT GGGCCTGACG
    GATGACCGAG ACATTTCTGA GACGGTCAGT GAGACTTGGA GCACTGATGT CTTGGGGAGT
    GACTTTGACC CCAACATGGA TGAGGACCGA CTGCAGGAGA TAGGGGCTGC AGCGGAGAAT
    GCTCTGGGCA GCATGCTGTG TTTGCCTGTG CTTTTGGATC CATATGGTTC AACCATCTCA
    GAGACCACGA GCGAGGCTTG GAGTGTGGAG GTTCTGCCGA GTGACTCAGA AGCTGCAGAC
    CTGAAGCAGG AGGAACGTTT GCAGGAGCTG GAGAGCTGCT CCGGTGTGGG AAGCACTTCA
    GACGACACAG AGGTCCGAGA GGTCAGCTCA AGACCAAGTA CTCCAGGACT CAGCGTTGTT
    TCAGGCATCA GTGCCACGTC GGAGGACATC CCTAATAAGA TTGAAGACCT ACGCTCGGAA
    TGCAGCTCCG ATTTTGGAGG CAAAGACTCC GTGACCAGTC CAGATGGAGA GGACTCTGCT
    CATGGAGCTC ACAGTCTCTC CTGTGCACCT TCTCAAACAG ATTCACTGCT GGCCATGTTC
    GATCCCCTCT CCTCTGGAGA AGGTTCGTGC ACTGGCACCA TAGTGCGACC CAAGGTACAC
    TATCCTCGTC CTCCTCACCC TCCGCCTGAC CCACCCATTC CAGAGGCCAG CATAACCAGC
    CACATGGAAC CCCGTCCGCC CCTCTTTCCT CCCCACCTGG CACAGGGTGA CCTGGAGCAC
    CCCAAGCAGA GGCACTCTTA CCCTGAGAGG CTGGTGAGGA GCCGCAGCAC AGATGTGGTG
    TCCGCTGGAC GCAGACCTAA CAGTGACCCC GGACTGAACC GCAGAACCAT GATGGAGGAG
    AGAGACCCGG CTGGAGCTTA CTCTACTGGG CCCACCTCCT CTCCCAGCAA GGATTCCCTC
    AAAGGAGAGT CTGAGGACAG GAAGGACAGT GATGATGAGA AGTCTGATAG GAACAGACCC
    TGGTGGAAGA AGCGCTTTGT TCCTGCCATT CCTAAAGTGC CGATTGCCGG GCTTCGGAAG
    CGGGACAAGG CAGAAAAAGA CGAGCAAGGA GCAGAGAGGG TCCCACAAGA TGAGCCTTCA
    CTGCTCAGAC AGAGCTCCAA AACCCAGTCA GCAGAGGCAA TTATGGACAA GTACATCAGG
    TACATGGAGA AACGACCCGC ACAGGCTGAC GGAGCAACTG CTGGAGGTTA TGTTGGACAA
    GAGTCTTGTC GAGAAGGGGA CAGTGAGCAC GACTCTCCTA AAGATGAAGC TCTGCAGAAC
    ATCTCTGCTG ATGATCTTCC GGATTCTGCC AGTCAGACGG CACCACCTCA GGACAAGACT
    TTCTCCTTCA GTGATATTAA GAAGAAGCTA CGCCTGGCAC TTTGCTCTGC AGATTCAGTT
    GTGTTTCCCA TCATTCCACC TGCAACAACA CGCAACGGTC TTCCAGATCA TGCAGATCCT
    GAAGACAACG AGATTGTGTG CTTTCTGAAG GTGCAGCTGG CGGAGGCCAT TAACCTGCAG
    GATAAGAACC AGATGGCACA GATCCAGGAG ACCACGCGCT GCGTCAGCCA CCTCGACCCG
    CGCACCTGCA GAAAACTGCT CACTGCCATG GCAGAGGACT ACAGGAAGCG GGCTCCATAC
    ATCGCCTATC TGACACGTTG TCGGCAGGGC CTGCAGACAT CTCAGGCTCA TCTGGAGAGA
    CTCCTGCAGA GAGTGTTGAG AGATAAAGAA GTGGCCAACC GCTACTTTAC CACTGTCTGT
    GTCCGACTAC TGCTGGAGCA CATGGAGTCC AAGATGCTTG ACTTCATTAA AGCATTTCAG
    GGATGCACAG CAGCTGATGA TAAGACAGCA GCCGTGGAGG ATTTCCTGCG TTACCTGTAT
    GGAGCCATGG CCCATGATGC CATCTGGCAG TACGCCAGTG AAGAGCAGCT CCAGGACGCA
    CAGATGGCCA TCGAACGCAG CGTCATGAAC CGCATCTTCA AACTGGCATT CTACCCCAAT
    CAGGATGGGG ACATCCACAG AGACGAGCTA TTCCATGAGC ACATTCAGCG ACTGTCTAGA
    GTGGTGTCGG CCAATCATAA AGCTCTGCAG ATCCCTGAGG TGTATCTGAA AGAGGCTCCG
    TGGCCTTCTG CACAGGCTGA GATCAAGACC ATCAACGCCT ATAAGACCCC GCGAGATAAA
    GTGCAGTGTA TCTTGCGCAT GTGCTCCACC ATTATGAACC TGCTCAGCCT GGCCAATGAG
    GATGCTGTAC CCGGCGCGGA TGACTTCGTC CCTGTGCTCG TCTTTGTTCT GATTAAAGCA
    AACCCACCCT GCCTTCTGTC CACCATTCAG TACATCAATA ATTTCTACGC CAGCAGGCTG
    AGCGGGGAGG AGTGCTATTG GTGGATGCAG TTCACTGCTG CCGTGGAATT CATTAAAACC
    ATCGACGATC GCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001002420.2
    CDS145..4482
    Translation

    Target ORF information:

    RefSeq Version NM_001002420.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001002420.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGCTCTC GCAGCCGAAG CCGATCCAAC ATGGCCCAGG AAGGAGAGAC TGAAGCCAGT
    TCTCAAGAGT CTCTGCAAGA GGTCCCCGAG GAAGTGCTGG TCATCTCTCT AGGAACATCA
    CCACAGACCA TTCCCGGGAT GATGTCAGAA AACGAGGTTC TGACTTTGCA TCTTGGAGAT
    GGAGGACAAG GTGACGCCCC TGTCGACGAC ACCAAATTAC ATGGCAAGCC TGACAAAACA
    CTGCGCTTCT CACTTTGCAG TGACAACCTG GAGGGCATTT CTGAGGGGCC GTCTAACCGC
    TCAAATTCAG TTTCTTCTTT GGATTTGGAA GTGGAGTCCG TGTCAGAAGG AGGACCAGGA
    CCGTCTGGTA GTAACGGTGC AGAAGCCTTA CAGCTACTGG AGCACGAACA AGCCACTACA
    CAGGATAATT TGGACGATAA ACTGCGCAAG TTTGAGATTC GGGACATGAT GGGCCTGACG
    GATGACCGAG ACATTTCTGA GACGGTCAGT GAGACTTGGA GCACTGATGT CTTGGGGAGT
    GACTTTGACC CCAACATGGA TGAGGACCGA CTGCAGGAGA TAGGGGCTGC AGCGGAGAAT
    GCTCTGGGCA GCATGCTGTG TTTGCCTGTG CTTTTGGATC CATATGGTTC AACCATCTCA
    GAGACCACGA GCGAGGCTTG GAGTGTGGAG GTTCTGCCGA GTGACTCAGA AGCTGCAGAC
    CTGAAGCAGG AGGAACGTTT GCAGGAGCTG GAGAGCTGCT CCGGTGTGGG AAGCACTTCA
    GACGACACAG AGGTCCGAGA GGTCAGCTCA AGACCAAGTA CTCCAGGACT CAGCGTTGTT
    TCAGGCATCA GTGCCACGTC GGAGGACATC CCTAATAAGA TTGAAGACCT ACGCTCGGAA
    TGCAGCTCCG ATTTTGGAGG CAAAGACTCC GTGACCAGTC CAGATGGAGA GGACTCTGCT
    CATGGAGCTC ACAGTCTCTC CTGTGCACCT TCTCAAACAG ATTCACTGCT GGCCATGTTC
    GATCCCCTCT CCTCTGGAGA AGGTTCGTGC ACTGGCACCA TAGTGCGACC CAAGGTACAC
    TATCCTCGTC CTCCTCACCC TCCGCCTGAC CCACCCATTC CAGAGGCCAG CATAACCAGC
    CACATGGAAC CCCGTCCGCC CCTCTTTCCT CCCCACCTGG CACAGGGTGA CCTGGAGCAC
    CCCAAGCAGA GGCACTCTTA CCCTGAGAGG CTGGTGAGGA GCCGCAGCAC AGATGTGGTG
    TCCGCTGGAC GCAGACCTAA CAGTGACCCC GGACTGAACC GCAGAACCAT GATGGAGGAG
    AGAGACCCGG CTGGAGCTTA CTCTACTGGG CCCACCTCCT CTCCCAGCAA GGATTCCCTC
    AAAGGAGAGT CTGAGGACAG GAAGGACAGT GATGATGAGA AGTCTGATAG GAACAGACCC
    TGGTGGAAGA AGCGCTTTGT TCCTGCCATT CCTAAAGTGC CGATTGCCGG GCTTCGGAAG
    CGGGACAAGG CAGAAAAAGA CGAGCAAGGA GCAGAGAGGG TCCCACAAGA TGAGCCTTCA
    CTGCTCAGAC AGAGCTCCAA AACCCAGTCA GCAGAGGCAA TTATGGACAA GTACATCAGG
    TACATGGAGA AACGACCCGC ACAGGCTGAC GGAGCAACTG CTGGAGGTTA TGTTGGACAA
    GAGTCTTGTC GAGAAGGGGA CAGTGAGCAC GACTCTCCTA AAGATGAAGC TCTGCAGAAC
    ATCTCTGCTG ATGATCTTCC GGATTCTGCC AGTCAGACGG CACCACCTCA GGACAAGACT
    TTCTCCTTCA GTGATATTAA GAAGAAGCTA CGCCTGGCAC TTTGCTCTGC AGATTCAGTT
    GTGTTTCCCA TCATTCCACC TGCAACAACA CGCAACGGTC TTCCAGATCA TGCAGATCCT
    GAAGACAACG AGATTGTGTG CTTTCTGAAG GTGCAGCTGG CGGAGGCCAT TAACCTGCAG
    GATAAGAACC AGATGGCACA GATCCAGGAG ACCACGCGCT GCGTCAGCCA CCTCGACCCG
    CGCACCTGCA GAAAACTGCT CACTGCCATG GCAGAGGACT ACAGGAAGCG GGCTCCATAC
    ATCGCCTATC TGACACGTTG TCGGCAGGGC CTGCAGACAT CTCAGGCTCA TCTGGAGAGA
    CTCCTGCAGA GAGTGTTGAG AGATAAAGAA GTGGCCAACC GCTACTTTAC CACTGTCTGT
    GTCCGACTAC TGCTGGAGCA CATGGAGTCC AAGATGCTTG ACTTCATTAA AGCATTTCAG
    GGATGCACAG CAGCTGATGA TAAGACAGCA GCCGTGGAGG ATTTCCTGCG TTACCTGTAT
    GGAGCCATGG CCCATGATGC CATCTGGCAG TACGCCAGTG AAGAGCAGCT CCAGGACGCA
    CAGATGGCCA TCGAACGCAG CGTCATGAAC CGCATCTTCA AACTGGCATT CTACCCCAAT
    CAGGATGGGG ACATCCACAG AGACGAGCTA TTCCATGAGC ACATTCAGCG ACTGTCTAGA
    GTGGTGTCGG CCAATCATAA AGCTCTGCAG ATCCCTGAGG TGTATCTGAA AGAGGCTCCG
    TGGCCTTCTG CACAGGCTGA GATCAAGACC ATCAACGCCT ATAAGACCCC GCGAGATAAA
    GTGCAGTGTA TCTTGCGCAT GTGCTCCACC ATTATGAACC TGCTCAGCCT GGCCAATGAG
    GATGCTGTAC CCGGCGCGGA TGACTTCGTC CCTGTGCTCG TCTTTGTTCT GATTAAAGCA
    AACCCACCCT GCCTTCTGTC CACCATTCAG TACATCAATA ATTTCTACGC CAGCAGGCTG
    AGCGGGGAGG AGTGCTATTG GTGGATGCAG TTCACTGCTG CCGTGGAATT CATTAAAACC
    ATCGACGATC GCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa14363
    Clone ID Related Accession (Same CDS sequence) XM_005167157.3 , XM_005167157.4
    Accession Version XM_005167157.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167157.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGACAAC AGTTGGCAGC CATAACATGT TGGGAGCCCT CTGCCACCTC CCTGTCCGCT
    CATATACCCT TAGTAACCCC GTTTGCAGGC TCTCGCAGCC GAAGCCGATC CAACATGGCC
    CAGGAAGGAG AGACTGAAGC CAGTTCTCAA GAGTCTCTGC AAGAGGTCCC CGAGGAAGTG
    CTGGTCATCT CTCTAGGAAC ATCACCACAG ACCATTCCCG GGATGATGTC AGAAAACGAG
    GTTCTGACTT TGCATCTTGG AGATGGAGGA CAAGGTGACG CCCCTGTCGA CGACACCAAA
    TTACATGGCA AGCCTGACAA AACACTGCGC TTCTCACTTT GCAGTGACAA CCTGGAGGGC
    ATTTCTGAGG GGCCGTCTAA CCGCTCAAAT TCAGTTTCTT CTTTGGATTT GGAAGTGGAG
    TCCGTGTCAG AAGGAGGACC AGGACCGTCT GGTAGTAACG GTGCAGAAGC CTTACAGCTA
    CTGGAGCACG AACAAGCCAC TACACAGGAT AATTTGGACG ATAAACTGCG CAAGTTTGAG
    ATTCGGGACA TGATGGGCCT GACGGATGAC CGAGACATTT CTGAGACGGT CAGTGAGACT
    TGGAGCACTG ATGTCTTGGG GAGTGACTTT GACCCCAACA TGGATGAGGA CCGACTGCAG
    GAGATAGGGG CTGCAGCGGA GAATGCTCTG GGCAGCATGC TGTGTTTGCC TGTGCTTTTG
    GATCCATATG GTTCAACCAT CTCAGAGACC ACGAGCGAGG CTTGGAGTGT GGAGGTTCTG
    CCGAGTGACT CAGAAGCTGC AGACCTGAAG CAGGAGGAAC GTTTGCAGGA GCTGGAGAGC
    TGCTCCGGTG TGGGAAGCAC TTCAGACGAC ACAGAGGTCC GAGAGGTCAG CTCAAGACCA
    AGTACTCCAG GACTCAGCGT TGTTTCAGGC ATCAGTGCCA CGTCGGAGGA CATCCCTAAT
    AAGATTGAAG ACCTACGCTC GGAATGCAGC TCCGATTTTG GAGGCAAAGA CTCCGTGACC
    AGTCCAGATG GAGAGGACTC TGCTCATGGA GCTCACAGTC TCTCCTGTGC ACCTTCTCAA
    ACAGATTCAC TGCTGGCCAT GTTCGATCCC CTCTCCTCTG GAGAAGGTTC GTGCACTGGC
    ACCATAGTGC GACCCAAGGT ACACTATCCT CGTCCTCCTC ACCCTCCGCC TGACCCACCC
    ATTCCAGAGG CCAGCATAAC CAGCCACATG GAACCCCGTC CGCCCCTCTT TCCTCCCCAC
    CTGGCACAGG GTGACCTGGA GCACCCCAAG CAGAGGCACT CTTACCCTGA GAGGCTGGTG
    AGGAGCCGCA GCACAGATGT GGTGTCCGCT GGACGCAGAC CTAACAGTGA CCCCGGACTG
    AACCGCAGAA CCATGATGGA GGAGAGAGAC CCGGCTGGAG CTTACTCTAC TGGGCCCACC
    TCCTCTCCCA GCAAGGATTC CCTCAAAGGA GAGTCTGAGG ACAGGAAGGA CAGTGATGAT
    GAGAAGTCTG ATAGGAACAG ACCCTGGTGG AAGAAGCGCT TTGTTCCTGC CATTCCTAAA
    GTGCCGATTG CCGGGCTTCG GAAGCGGGAC AAGGCAGAAA AAGACGAGCA AGGAGCAGAG
    AGGGTCCCAC AAGATGAGCC TTCACTGCTC AGACAGAGCT CCAAAACCCA GTCAGCAGAG
    GCAATTATGG ACAAGTACAT CAGGTACATG GAGAAACGAC CCGCACAGGC TGACGGAGCA
    ACTGCTGGAG GTTATGTTGG ACAAGAGTCT TGTCGAGAAG GGGACAGTGA GCACGACTCT
    CCTAAAGATG AAGCTCTGCA GAACATCTCT GCTGATGATC TTCCGGATTC TGCCAGTCAG
    ACGGCACCAC CTCAGGACAA GACTTTCTCC TTCAGTGATA TTAAGAAGAA GCTACGCCTG
    GCACTTTGCT CTGCAGATTC AGTTGTGTTT CCCATCATTC CACCTGCAAC AACACGCAAC
    GGTCTTCCAG ATCATGCAGA TCCTGAAGAC AACGAGATTG TGTGCTTTCT GAAGGTGCAG
    CTGGCGGAGG CCATTAACCT GCAGGATAAG AACCAGATGG CACAGATCCA GGAGACCACG
    CGCTGCGTCA GCCACCTCGA CCCGCGCACC TGCAGAAAAC TGCTCACTGC CATGGCAGAG
    GACTACAGGA AGCGGGCTCC ATACATCGCC TATCTGACAC GTTGTCGGCA GGGCCTGCAG
    ACATCTCAGG CTCATCTGGA GAGACTCCTG CAGAGAGTGT TGAGAGATAA AGAAGTGGCC
    AACCGCTACT TTACCACTGT CTGTGTCCGA CTACTGCTGG AGCACATGGA GTCCAAGATG
    CTTGACTTCA TTAAAGCATT TCAGGGATGC ACAGCAGCTG ATGATAAGAC AGCAGCCGTG
    GAGGATTTCC TGCGTTACCT GTATGGAGCC ATGGCCCATG ATGCCATCTG GCAGTACGCC
    AGTGAAGAGC AGCTCCAGGA CGCACAGATG GCCATCGAAC GCAGCGTCAT GAACCGCATC
    TTCAAACTGG CATTCTACCC CAATCAGGAT GGGGACATCC ACAGAGACGA GCTATTCCAT
    GAGCACATTC AGCGACTGTC TAGAGTGGTG TCGGCCAATC ATAAAGCTCT GCAGATCCCT
    GAGGTGTATC TGAAAGAGGC TCCGTGGCCT TCTGCACAGG CTGAGATCAA GACCATCAAC
    GCCTATAAGA CCCCGCGAGA TAAAGTGCAG TGTATCTTGC GCATGTGCTC CACCATTATG
    AACCTGCTCA GCCTGGCCAA TGAGGATGCT GTACCCGGCG CGGATGACTT CGTCCCTGTG
    CTCGTCTTTG TTCTGATTAA AGCAAACCCA CCCTGCCTTC TGTCCACCAT TCAGTACATC
    AATAATTTCT ACGCCAGCAG GCTGAGCGGG GAGGAGTGCT ATTGGTGGAT GCAGTTCACT
    GCTGCCGTGG AATTCATTAA AACCATCGAC GATCGCAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005167214.1
    CDS200..4621
    Translation

    Target ORF information:

    RefSeq Version XM_005167157.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005167157.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGACAAC AGTTGGCAGC CATAACATGT TGGGAGCCCT CTGCCACCTC CCTGTCCGCT
    CATATACCCT TAGTAACCCC GTTTGCAGGC TCTCGCAGCC GAAGCCGATC CAACATGGCC
    CAGGAAGGAG AGACTGAAGC CAGTTCTCAA GAGTCTCTGC AAGAGGTCCC CGAGGAAGTG
    CTGGTCATCT CTCTAGGAAC ATCACCACAG ACCATTCCCG GGATGATGTC AGAAAACGAG
    GTTCTGACTT TGCATCTTGG AGATGGAGGA CAAGGTGACG CCCCTGTCGA CGACACCAAA
    TTACATGGCA AGCCTGACAA AACACTGCGC TTCTCACTTT GCAGTGACAA CCTGGAGGGC
    ATTTCTGAGG GGCCGTCTAA CCGCTCAAAT TCAGTTTCTT CTTTGGATTT GGAAGTGGAG
    TCCGTGTCAG AAGGAGGACC AGGACCGTCT GGTAGTAACG GTGCAGAAGC CTTACAGCTA
    CTGGAGCACG AACAAGCCAC TACACAGGAT AATTTGGACG ATAAACTGCG CAAGTTTGAG
    ATTCGGGACA TGATGGGCCT GACGGATGAC CGAGACATTT CTGAGACGGT CAGTGAGACT
    TGGAGCACTG ATGTCTTGGG GAGTGACTTT GACCCCAACA TGGATGAGGA CCGACTGCAG
    GAGATAGGGG CTGCAGCGGA GAATGCTCTG GGCAGCATGC TGTGTTTGCC TGTGCTTTTG
    GATCCATATG GTTCAACCAT CTCAGAGACC ACGAGCGAGG CTTGGAGTGT GGAGGTTCTG
    CCGAGTGACT CAGAAGCTGC AGACCTGAAG CAGGAGGAAC GTTTGCAGGA GCTGGAGAGC
    TGCTCCGGTG TGGGAAGCAC TTCAGACGAC ACAGAGGTCC GAGAGGTCAG CTCAAGACCA
    AGTACTCCAG GACTCAGCGT TGTTTCAGGC ATCAGTGCCA CGTCGGAGGA CATCCCTAAT
    AAGATTGAAG ACCTACGCTC GGAATGCAGC TCCGATTTTG GAGGCAAAGA CTCCGTGACC
    AGTCCAGATG GAGAGGACTC TGCTCATGGA GCTCACAGTC TCTCCTGTGC ACCTTCTCAA
    ACAGATTCAC TGCTGGCCAT GTTCGATCCC CTCTCCTCTG GAGAAGGTTC GTGCACTGGC
    ACCATAGTGC GACCCAAGGT ACACTATCCT CGTCCTCCTC ACCCTCCGCC TGACCCACCC
    ATTCCAGAGG CCAGCATAAC CAGCCACATG GAACCCCGTC CGCCCCTCTT TCCTCCCCAC
    CTGGCACAGG GTGACCTGGA GCACCCCAAG CAGAGGCACT CTTACCCTGA GAGGCTGGTG
    AGGAGCCGCA GCACAGATGT GGTGTCCGCT GGACGCAGAC CTAACAGTGA CCCCGGACTG
    AACCGCAGAA CCATGATGGA GGAGAGAGAC CCGGCTGGAG CTTACTCTAC TGGGCCCACC
    TCCTCTCCCA GCAAGGATTC CCTCAAAGGA GAGTCTGAGG ACAGGAAGGA CAGTGATGAT
    GAGAAGTCTG ATAGGAACAG ACCCTGGTGG AAGAAGCGCT TTGTTCCTGC CATTCCTAAA
    GTGCCGATTG CCGGGCTTCG GAAGCGGGAC AAGGCAGAAA AAGACGAGCA AGGAGCAGAG
    AGGGTCCCAC AAGATGAGCC TTCACTGCTC AGACAGAGCT CCAAAACCCA GTCAGCAGAG
    GCAATTATGG ACAAGTACAT CAGGTACATG GAGAAACGAC CCGCACAGGC TGACGGAGCA
    ACTGCTGGAG GTTATGTTGG ACAAGAGTCT TGTCGAGAAG GGGACAGTGA GCACGACTCT
    CCTAAAGATG AAGCTCTGCA GAACATCTCT GCTGATGATC TTCCGGATTC TGCCAGTCAG
    ACGGCACCAC CTCAGGACAA GACTTTCTCC TTCAGTGATA TTAAGAAGAA GCTACGCCTG
    GCACTTTGCT CTGCAGATTC AGTTGTGTTT CCCATCATTC CACCTGCAAC AACACGCAAC
    GGTCTTCCAG ATCATGCAGA TCCTGAAGAC AACGAGATTG TGTGCTTTCT GAAGGTGCAG
    CTGGCGGAGG CCATTAACCT GCAGGATAAG AACCAGATGG CACAGATCCA GGAGACCACG
    CGCTGCGTCA GCCACCTCGA CCCGCGCACC TGCAGAAAAC TGCTCACTGC CATGGCAGAG
    GACTACAGGA AGCGGGCTCC ATACATCGCC TATCTGACAC GTTGTCGGCA GGGCCTGCAG
    ACATCTCAGG CTCATCTGGA GAGACTCCTG CAGAGAGTGT TGAGAGATAA AGAAGTGGCC
    AACCGCTACT TTACCACTGT CTGTGTCCGA CTACTGCTGG AGCACATGGA GTCCAAGATG
    CTTGACTTCA TTAAAGCATT TCAGGGATGC ACAGCAGCTG ATGATAAGAC AGCAGCCGTG
    GAGGATTTCC TGCGTTACCT GTATGGAGCC ATGGCCCATG ATGCCATCTG GCAGTACGCC
    AGTGAAGAGC AGCTCCAGGA CGCACAGATG GCCATCGAAC GCAGCGTCAT GAACCGCATC
    TTCAAACTGG CATTCTACCC CAATCAGGAT GGGGACATCC ACAGAGACGA GCTATTCCAT
    GAGCACATTC AGCGACTGTC TAGAGTGGTG TCGGCCAATC ATAAAGCTCT GCAGATCCCT
    GAGGTGTATC TGAAAGAGGC TCCGTGGCCT TCTGCACAGG CTGAGATCAA GACCATCAAC
    GCCTATAAGA CCCCGCGAGA TAAAGTGCAG TGTATCTTGC GCATGTGCTC CACCATTATG
    AACCTGCTCA GCCTGGCCAA TGAGGATGCT GTACCCGGCG CGGATGACTT CGTCCCTGTG
    CTCGTCTTTG TTCTGATTAA AGCAAACCCA CCCTGCCTTC TGTCCACCAT TCAGTACATC
    AATAATTTCT ACGCCAGCAG GCTGAGCGGG GAGGAGTGCT ATTGGTGGAT GCAGTTCACT
    GCTGCCGTGG AATTCATTAA AACCATCGAC GATCGCAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa14363
    Clone ID Related Accession (Same CDS sequence) XM_005167157.3 , XM_005167157.4
    Accession Version XM_005167157.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167157.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGACAAC AGTTGGCAGC CATAACATGT TGGGAGCCCT CTGCCACCTC CCTGTCCGCT
    CATATACCCT TAGTAACCCC GTTTGCAGGC TCTCGCAGCC GAAGCCGATC CAACATGGCC
    CAGGAAGGAG AGACTGAAGC CAGTTCTCAA GAGTCTCTGC AAGAGGTCCC CGAGGAAGTG
    CTGGTCATCT CTCTAGGAAC ATCACCACAG ACCATTCCCG GGATGATGTC AGAAAACGAG
    GTTCTGACTT TGCATCTTGG AGATGGAGGA CAAGGTGACG CCCCTGTCGA CGACACCAAA
    TTACATGGCA AGCCTGACAA AACACTGCGC TTCTCACTTT GCAGTGACAA CCTGGAGGGC
    ATTTCTGAGG GGCCGTCTAA CCGCTCAAAT TCAGTTTCTT CTTTGGATTT GGAAGTGGAG
    TCCGTGTCAG AAGGAGGACC AGGACCGTCT GGTAGTAACG GTGCAGAAGC CTTACAGCTA
    CTGGAGCACG AACAAGCCAC TACACAGGAT AATTTGGACG ATAAACTGCG CAAGTTTGAG
    ATTCGGGACA TGATGGGCCT GACGGATGAC CGAGACATTT CTGAGACGGT CAGTGAGACT
    TGGAGCACTG ATGTCTTGGG GAGTGACTTT GACCCCAACA TGGATGAGGA CCGACTGCAG
    GAGATAGGGG CTGCAGCGGA GAATGCTCTG GGCAGCATGC TGTGTTTGCC TGTGCTTTTG
    GATCCATATG GTTCAACCAT CTCAGAGACC ACGAGCGAGG CTTGGAGTGT GGAGGTTCTG
    CCGAGTGACT CAGAAGCTGC AGACCTGAAG CAGGAGGAAC GTTTGCAGGA GCTGGAGAGC
    TGCTCCGGTG TGGGAAGCAC TTCAGACGAC ACAGAGGTCC GAGAGGTCAG CTCAAGACCA
    AGTACTCCAG GACTCAGCGT TGTTTCAGGC ATCAGTGCCA CGTCGGAGGA CATCCCTAAT
    AAGATTGAAG ACCTACGCTC GGAATGCAGC TCCGATTTTG GAGGCAAAGA CTCCGTGACC
    AGTCCAGATG GAGAGGACTC TGCTCATGGA GCTCACAGTC TCTCCTGTGC ACCTTCTCAA
    ACAGATTCAC TGCTGGCCAT GTTCGATCCC CTCTCCTCTG GAGAAGGTTC GTGCACTGGC
    ACCATAGTGC GACCCAAGGT ACACTATCCT CGTCCTCCTC ACCCTCCGCC TGACCCACCC
    ATTCCAGAGG CCAGCATAAC CAGCCACATG GAACCCCGTC CGCCCCTCTT TCCTCCCCAC
    CTGGCACAGG GTGACCTGGA GCACCCCAAG CAGAGGCACT CTTACCCTGA GAGGCTGGTG
    AGGAGCCGCA GCACAGATGT GGTGTCCGCT GGACGCAGAC CTAACAGTGA CCCCGGACTG
    AACCGCAGAA CCATGATGGA GGAGAGAGAC CCGGCTGGAG CTTACTCTAC TGGGCCCACC
    TCCTCTCCCA GCAAGGATTC CCTCAAAGGA GAGTCTGAGG ACAGGAAGGA CAGTGATGAT
    GAGAAGTCTG ATAGGAACAG ACCCTGGTGG AAGAAGCGCT TTGTTCCTGC CATTCCTAAA
    GTGCCGATTG CCGGGCTTCG GAAGCGGGAC AAGGCAGAAA AAGACGAGCA AGGAGCAGAG
    AGGGTCCCAC AAGATGAGCC TTCACTGCTC AGACAGAGCT CCAAAACCCA GTCAGCAGAG
    GCAATTATGG ACAAGTACAT CAGGTACATG GAGAAACGAC CCGCACAGGC TGACGGAGCA
    ACTGCTGGAG GTTATGTTGG ACAAGAGTCT TGTCGAGAAG GGGACAGTGA GCACGACTCT
    CCTAAAGATG AAGCTCTGCA GAACATCTCT GCTGATGATC TTCCGGATTC TGCCAGTCAG
    ACGGCACCAC CTCAGGACAA GACTTTCTCC TTCAGTGATA TTAAGAAGAA GCTACGCCTG
    GCACTTTGCT CTGCAGATTC AGTTGTGTTT CCCATCATTC CACCTGCAAC AACACGCAAC
    GGTCTTCCAG ATCATGCAGA TCCTGAAGAC AACGAGATTG TGTGCTTTCT GAAGGTGCAG
    CTGGCGGAGG CCATTAACCT GCAGGATAAG AACCAGATGG CACAGATCCA GGAGACCACG
    CGCTGCGTCA GCCACCTCGA CCCGCGCACC TGCAGAAAAC TGCTCACTGC CATGGCAGAG
    GACTACAGGA AGCGGGCTCC ATACATCGCC TATCTGACAC GTTGTCGGCA GGGCCTGCAG
    ACATCTCAGG CTCATCTGGA GAGACTCCTG CAGAGAGTGT TGAGAGATAA AGAAGTGGCC
    AACCGCTACT TTACCACTGT CTGTGTCCGA CTACTGCTGG AGCACATGGA GTCCAAGATG
    CTTGACTTCA TTAAAGCATT TCAGGGATGC ACAGCAGCTG ATGATAAGAC AGCAGCCGTG
    GAGGATTTCC TGCGTTACCT GTATGGAGCC ATGGCCCATG ATGCCATCTG GCAGTACGCC
    AGTGAAGAGC AGCTCCAGGA CGCACAGATG GCCATCGAAC GCAGCGTCAT GAACCGCATC
    TTCAAACTGG CATTCTACCC CAATCAGGAT GGGGACATCC ACAGAGACGA GCTATTCCAT
    GAGCACATTC AGCGACTGTC TAGAGTGGTG TCGGCCAATC ATAAAGCTCT GCAGATCCCT
    GAGGTGTATC TGAAAGAGGC TCCGTGGCCT TCTGCACAGG CTGAGATCAA GACCATCAAC
    GCCTATAAGA CCCCGCGAGA TAAAGTGCAG TGTATCTTGC GCATGTGCTC CACCATTATG
    AACCTGCTCA GCCTGGCCAA TGAGGATGCT GTACCCGGCG CGGATGACTT CGTCCCTGTG
    CTCGTCTTTG TTCTGATTAA AGCAAACCCA CCCTGCCTTC TGTCCACCAT TCAGTACATC
    AATAATTTCT ACGCCAGCAG GCTGAGCGGG GAGGAGTGCT ATTGGTGGAT GCAGTTCACT
    GCTGCCGTGG AATTCATTAA AACCATCGAC GATCGCAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005167214.1
    CDS202..4623
    Translation

    Target ORF information:

    RefSeq Version XM_005167157.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005167157.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGACAAC AGTTGGCAGC CATAACATGT TGGGAGCCCT CTGCCACCTC CCTGTCCGCT
    CATATACCCT TAGTAACCCC GTTTGCAGGC TCTCGCAGCC GAAGCCGATC CAACATGGCC
    CAGGAAGGAG AGACTGAAGC CAGTTCTCAA GAGTCTCTGC AAGAGGTCCC CGAGGAAGTG
    CTGGTCATCT CTCTAGGAAC ATCACCACAG ACCATTCCCG GGATGATGTC AGAAAACGAG
    GTTCTGACTT TGCATCTTGG AGATGGAGGA CAAGGTGACG CCCCTGTCGA CGACACCAAA
    TTACATGGCA AGCCTGACAA AACACTGCGC TTCTCACTTT GCAGTGACAA CCTGGAGGGC
    ATTTCTGAGG GGCCGTCTAA CCGCTCAAAT TCAGTTTCTT CTTTGGATTT GGAAGTGGAG
    TCCGTGTCAG AAGGAGGACC AGGACCGTCT GGTAGTAACG GTGCAGAAGC CTTACAGCTA
    CTGGAGCACG AACAAGCCAC TACACAGGAT AATTTGGACG ATAAACTGCG CAAGTTTGAG
    ATTCGGGACA TGATGGGCCT GACGGATGAC CGAGACATTT CTGAGACGGT CAGTGAGACT
    TGGAGCACTG ATGTCTTGGG GAGTGACTTT GACCCCAACA TGGATGAGGA CCGACTGCAG
    GAGATAGGGG CTGCAGCGGA GAATGCTCTG GGCAGCATGC TGTGTTTGCC TGTGCTTTTG
    GATCCATATG GTTCAACCAT CTCAGAGACC ACGAGCGAGG CTTGGAGTGT GGAGGTTCTG
    CCGAGTGACT CAGAAGCTGC AGACCTGAAG CAGGAGGAAC GTTTGCAGGA GCTGGAGAGC
    TGCTCCGGTG TGGGAAGCAC TTCAGACGAC ACAGAGGTCC GAGAGGTCAG CTCAAGACCA
    AGTACTCCAG GACTCAGCGT TGTTTCAGGC ATCAGTGCCA CGTCGGAGGA CATCCCTAAT
    AAGATTGAAG ACCTACGCTC GGAATGCAGC TCCGATTTTG GAGGCAAAGA CTCCGTGACC
    AGTCCAGATG GAGAGGACTC TGCTCATGGA GCTCACAGTC TCTCCTGTGC ACCTTCTCAA
    ACAGATTCAC TGCTGGCCAT GTTCGATCCC CTCTCCTCTG GAGAAGGTTC GTGCACTGGC
    ACCATAGTGC GACCCAAGGT ACACTATCCT CGTCCTCCTC ACCCTCCGCC TGACCCACCC
    ATTCCAGAGG CCAGCATAAC CAGCCACATG GAACCCCGTC CGCCCCTCTT TCCTCCCCAC
    CTGGCACAGG GTGACCTGGA GCACCCCAAG CAGAGGCACT CTTACCCTGA GAGGCTGGTG
    AGGAGCCGCA GCACAGATGT GGTGTCCGCT GGACGCAGAC CTAACAGTGA CCCCGGACTG
    AACCGCAGAA CCATGATGGA GGAGAGAGAC CCGGCTGGAG CTTACTCTAC TGGGCCCACC
    TCCTCTCCCA GCAAGGATTC CCTCAAAGGA GAGTCTGAGG ACAGGAAGGA CAGTGATGAT
    GAGAAGTCTG ATAGGAACAG ACCCTGGTGG AAGAAGCGCT TTGTTCCTGC CATTCCTAAA
    GTGCCGATTG CCGGGCTTCG GAAGCGGGAC AAGGCAGAAA AAGACGAGCA AGGAGCAGAG
    AGGGTCCCAC AAGATGAGCC TTCACTGCTC AGACAGAGCT CCAAAACCCA GTCAGCAGAG
    GCAATTATGG ACAAGTACAT CAGGTACATG GAGAAACGAC CCGCACAGGC TGACGGAGCA
    ACTGCTGGAG GTTATGTTGG ACAAGAGTCT TGTCGAGAAG GGGACAGTGA GCACGACTCT
    CCTAAAGATG AAGCTCTGCA GAACATCTCT GCTGATGATC TTCCGGATTC TGCCAGTCAG
    ACGGCACCAC CTCAGGACAA GACTTTCTCC TTCAGTGATA TTAAGAAGAA GCTACGCCTG
    GCACTTTGCT CTGCAGATTC AGTTGTGTTT CCCATCATTC CACCTGCAAC AACACGCAAC
    GGTCTTCCAG ATCATGCAGA TCCTGAAGAC AACGAGATTG TGTGCTTTCT GAAGGTGCAG
    CTGGCGGAGG CCATTAACCT GCAGGATAAG AACCAGATGG CACAGATCCA GGAGACCACG
    CGCTGCGTCA GCCACCTCGA CCCGCGCACC TGCAGAAAAC TGCTCACTGC CATGGCAGAG
    GACTACAGGA AGCGGGCTCC ATACATCGCC TATCTGACAC GTTGTCGGCA GGGCCTGCAG
    ACATCTCAGG CTCATCTGGA GAGACTCCTG CAGAGAGTGT TGAGAGATAA AGAAGTGGCC
    AACCGCTACT TTACCACTGT CTGTGTCCGA CTACTGCTGG AGCACATGGA GTCCAAGATG
    CTTGACTTCA TTAAAGCATT TCAGGGATGC ACAGCAGCTG ATGATAAGAC AGCAGCCGTG
    GAGGATTTCC TGCGTTACCT GTATGGAGCC ATGGCCCATG ATGCCATCTG GCAGTACGCC
    AGTGAAGAGC AGCTCCAGGA CGCACAGATG GCCATCGAAC GCAGCGTCAT GAACCGCATC
    TTCAAACTGG CATTCTACCC CAATCAGGAT GGGGACATCC ACAGAGACGA GCTATTCCAT
    GAGCACATTC AGCGACTGTC TAGAGTGGTG TCGGCCAATC ATAAAGCTCT GCAGATCCCT
    GAGGTGTATC TGAAAGAGGC TCCGTGGCCT TCTGCACAGG CTGAGATCAA GACCATCAAC
    GCCTATAAGA CCCCGCGAGA TAAAGTGCAG TGTATCTTGC GCATGTGCTC CACCATTATG
    AACCTGCTCA GCCTGGCCAA TGAGGATGCT GTACCCGGCG CGGATGACTT CGTCCCTGTG
    CTCGTCTTTG TTCTGATTAA AGCAAACCCA CCCTGCCTTC TGTCCACCAT TCAGTACATC
    AATAATTTCT ACGCCAGCAG GCTGAGCGGG GAGGAGTGCT ATTGGTGGAT GCAGTTCACT
    GCTGCCGTGG AATTCATTAA AACCATCGAC GATCGCAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41043
    Clone ID Related Accession (Same CDS sequence) XM_009304093.3 , XM_009304093.2
    Accession Version XM_009304093.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304093.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGACAAC AGTTGGCAGC CATAACATGT TGGGAGCCCT CTGCCACCTC CCTGTCCGCT
    CATATACCCT TAGTAACCCC GTTTGCAGGC TCTCGCAGCC GAAGCCGATC CAACATGGCC
    CAGGAAGGAG AGACTGAAGC CAGTTCTCAA GAGTCTCTGC AAGAGGTCCC CGAGGAAGTG
    CTGGTCATCT CTCTAGGAAC ATCACCACAG ACCATTCCCG GGATGATGTC AGAAAACGAG
    GTTCTGACTT TGCATCTTGG AGATGGAGGA CAAGGTGACG CCCCTGTCGA CGACACCAAA
    TTACATGGCA AGCCTGACAA AACACTGCGC TTCTCACTTT GCAGTGACAA CCTGGAGGGC
    ATTTCTGAGG GGCCGTCTAA CCGCTCAAAT TCAGTTTCTT CTTTGGATTT GGAAGTGGAG
    TCCGTGTCAG AAGGAGGACC AGGACCGTCT GGTAGTAACG GTGCAGAAGC CTTACAGCTA
    CTGGAGCACG AACAAGCCAC TACACAGGAT AATTTGGACG ATAAACTGCG CAAGTTTGAG
    ATTCGGGACA TGATGGGCCT GACGGATGAC CGAGACATTT CTGAGACGGT CAGTGAGACT
    TGGAGCACTG ATGTCTTGGG GAGTGACTTT GACCCCAACA TGGATGAGGA CCGACTGCAG
    GAGATAGGGG CTGCAGCGGA GAATGCTCTG GGCAGCATGC TGTGTTTGCC TGTGCTTTTG
    GATCCATATG GTTCAACCAT CTCAGAGACC ACGAGCGAGG CTTGGAGTGT GGAGGTTCTG
    CCGAGTGACT CAGAAGCTGC AGACCTGAAG CAGGAGGAAC GTTTGCAGGA GCTGGAGAGC
    TGCTCCGGTG TGGGAAGCAC TTCAGACGAC ACAGAGGTCC GAGAGGTCAG CTCAAGACCA
    AGTACTCCAG GACTCAGCGT TGTTTCAGGC ATCAGTGCCA CGTCGGAGGA CATCCCTAAT
    AAGATTGAAG ACCTACGCTC GGAATGCAGC TCCGATTTTG GAGGCAAAGA CTCCGTGACC
    AGTCCAGATG GAGAGGACTC TGCTCATGGA GCTCACAGTC TCTCCTGTGC ACCTTCTCAA
    ACAGATTCAC TGCTGGCCAT GTTCGATCCC CTCTCCTCTG GAGAAGGTTC GTGCACTGGC
    ACCATAGTGC GACCCAAGGT ACACTATCCT CGTCCTCCTC ACCCTCCGCC TGACCCACCC
    ATTCCAGAGG CCAGCATAAC CAGCCACATG GAACCCCGTC CGCCCCTCTT TCCTCCCCAC
    CTGGCACAGG GTGACCTGGA GCACCCCAAG CAGAGGCACT CTTACCCTGA GAGGCTGGTG
    AGGAGCCGCA GCACAGATGT GGTGTCCGCT GGACGCAGAC CTAACAGTGA CCCCGGACTG
    AACCGCAGAA CCATGATGGA GGAGAGAGAC CCGGCTGGAG CTTACTCTAC TGGGCCCACC
    TCCTCTCCCA GCAAGGATTC CCTCAAAGGA GAGTCTGAGG ACAGGAAGGA CAGTGATGAT
    GAGAAGTCTG ATAGGAACAG ACCCTGGTGG AAGAAGCGCT TTGTTCCTGC CATTCCTAAA
    GTGCTCTATT GGACGGCAGA GGGTGAACTC CCAGTGCCGA TTGCCGGGCT TCGGAAGCGG
    GACAAGGCAG AAAAAGACGA GCAAGGAGCA GAGAGGGTCC CACAAGATGA GCCTTCACTG
    CTCAGACAGA GCTCCAAAAC CCAGTCAGCA GAGGCAATTA TGGACAAGTA CATCAGGTAC
    ATGGAGAAAC GACCCGCACA GGCTGACGGA GCAACTGCTG GAGGTTATGT TGGACAAGAG
    TCTTGTCGAG AAGGGGACAG TGAGCACGAC TCTCCTAAAG ATGAAGCTCT GCAGAACATC
    TCTGCTGATG ATCTTCCGGA TTCTGCCAGT CAGACGGCAC CACCTCAGGA CAAGACTTTC
    TCCTTCAGTG ATATTAAGAA GAAGCTACGC CTGGCACTTT GCTCTGCAGA TTCAGTTGTG
    TTTCCCATCA TTCCACCTGC AACAACACGC AACGGTCTTC CAGATCATGC AGATCCTGAA
    GACAACGAGA TTGTGTGCTT TCTGAAGGTG CAGCTGGCGG AGGCCATTAA CCTGCAGGAT
    AAGAACCAGA TGGCACAGAT CCAGGAGACC ACGCGCTGCG TCAGCCACCT CGACCCGCGC
    ACCTGCAGAA AACTGCTCAC TGCCATGGCA GAGGACTACA GGAAGCGGGC TCCATACATC
    GCCTATCTGA CACGTTGTCG GCAGGGCCTG CAGACATCTC AGGCTCATCT GGAGAGACTC
    CTGCAGAGAG TGTTGAGAGA TAAAGAAGTG GCCAACCGCT ACTTTACCAC TGTCTGTGTC
    CGACTACTGC TGGAGCACAT GGAGTCCAAG ATGCTTGACT TCATTAAAGC ATTTCAGGGA
    TGCACAGCAG CTGATGATAA GACAGCAGCC GTGGAGGATT TCCTGCGTTA CCTGTATGGA
    GCCATGGCCC ATGATGCCAT CTGGCAGTAC GCCAGTGAAG AGCAGCTCCA GGACGCACAG
    ATGGCCATCG AACGCAGCGT CATGAACCGC ATCTTCAAAC TGGCATTCTA CCCCAATCAG
    GATGGGGACA TCCACAGAGA CGAGCTATTC CATGAGCACA TTCAGCGACT GTCTAGAGTG
    GTGTCGGCCA ATCATAAAGC TCTGCAGATC CCTGAGGTGT ATCTGAAAGA GGCTCCGTGG
    CCTTCTGCAC AGGCTGAGAT CAAGACCATC AACGCCTATA AGACCCCGCG AGATAAAGTG
    CAGTGTATCT TGCGCATGTG CTCCACCATT ATGAACCTGC TCAGCCTGGC CAATGAGGAT
    GCTGTACCCG GCGCGGATGA CTTCGTCCCT GTGCTCGTCT TTGTTCTGAT TAAAGCAAAC
    CCACCCTGCC TTCTGTCCAC CATTCAGTAC ATCAATAATT TCTACGCCAG CAGGCTGAGC
    GGGGAGGAGT GCTATTGGTG GATGCAGTTC ACTGCTGCCG TGGAATTCAT TAAAACCATC
    GACGATCGCA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302368.1
    CDS202..4656
    Translation

    Target ORF information:

    RefSeq Version XM_009304093.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304093.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGACAAC AGTTGGCAGC CATAACATGT TGGGAGCCCT CTGCCACCTC CCTGTCCGCT
    CATATACCCT TAGTAACCCC GTTTGCAGGC TCTCGCAGCC GAAGCCGATC CAACATGGCC
    CAGGAAGGAG AGACTGAAGC CAGTTCTCAA GAGTCTCTGC AAGAGGTCCC CGAGGAAGTG
    CTGGTCATCT CTCTAGGAAC ATCACCACAG ACCATTCCCG GGATGATGTC AGAAAACGAG
    GTTCTGACTT TGCATCTTGG AGATGGAGGA CAAGGTGACG CCCCTGTCGA CGACACCAAA
    TTACATGGCA AGCCTGACAA AACACTGCGC TTCTCACTTT GCAGTGACAA CCTGGAGGGC
    ATTTCTGAGG GGCCGTCTAA CCGCTCAAAT TCAGTTTCTT CTTTGGATTT GGAAGTGGAG
    TCCGTGTCAG AAGGAGGACC AGGACCGTCT GGTAGTAACG GTGCAGAAGC CTTACAGCTA
    CTGGAGCACG AACAAGCCAC TACACAGGAT AATTTGGACG ATAAACTGCG CAAGTTTGAG
    ATTCGGGACA TGATGGGCCT GACGGATGAC CGAGACATTT CTGAGACGGT CAGTGAGACT
    TGGAGCACTG ATGTCTTGGG GAGTGACTTT GACCCCAACA TGGATGAGGA CCGACTGCAG
    GAGATAGGGG CTGCAGCGGA GAATGCTCTG GGCAGCATGC TGTGTTTGCC TGTGCTTTTG
    GATCCATATG GTTCAACCAT CTCAGAGACC ACGAGCGAGG CTTGGAGTGT GGAGGTTCTG
    CCGAGTGACT CAGAAGCTGC AGACCTGAAG CAGGAGGAAC GTTTGCAGGA GCTGGAGAGC
    TGCTCCGGTG TGGGAAGCAC TTCAGACGAC ACAGAGGTCC GAGAGGTCAG CTCAAGACCA
    AGTACTCCAG GACTCAGCGT TGTTTCAGGC ATCAGTGCCA CGTCGGAGGA CATCCCTAAT
    AAGATTGAAG ACCTACGCTC GGAATGCAGC TCCGATTTTG GAGGCAAAGA CTCCGTGACC
    AGTCCAGATG GAGAGGACTC TGCTCATGGA GCTCACAGTC TCTCCTGTGC ACCTTCTCAA
    ACAGATTCAC TGCTGGCCAT GTTCGATCCC CTCTCCTCTG GAGAAGGTTC GTGCACTGGC
    ACCATAGTGC GACCCAAGGT ACACTATCCT CGTCCTCCTC ACCCTCCGCC TGACCCACCC
    ATTCCAGAGG CCAGCATAAC CAGCCACATG GAACCCCGTC CGCCCCTCTT TCCTCCCCAC
    CTGGCACAGG GTGACCTGGA GCACCCCAAG CAGAGGCACT CTTACCCTGA GAGGCTGGTG
    AGGAGCCGCA GCACAGATGT GGTGTCCGCT GGACGCAGAC CTAACAGTGA CCCCGGACTG
    AACCGCAGAA CCATGATGGA GGAGAGAGAC CCGGCTGGAG CTTACTCTAC TGGGCCCACC
    TCCTCTCCCA GCAAGGATTC CCTCAAAGGA GAGTCTGAGG ACAGGAAGGA CAGTGATGAT
    GAGAAGTCTG ATAGGAACAG ACCCTGGTGG AAGAAGCGCT TTGTTCCTGC CATTCCTAAA
    GTGCTCTATT GGACGGCAGA GGGTGAACTC CCAGTGCCGA TTGCCGGGCT TCGGAAGCGG
    GACAAGGCAG AAAAAGACGA GCAAGGAGCA GAGAGGGTCC CACAAGATGA GCCTTCACTG
    CTCAGACAGA GCTCCAAAAC CCAGTCAGCA GAGGCAATTA TGGACAAGTA CATCAGGTAC
    ATGGAGAAAC GACCCGCACA GGCTGACGGA GCAACTGCTG GAGGTTATGT TGGACAAGAG
    TCTTGTCGAG AAGGGGACAG TGAGCACGAC TCTCCTAAAG ATGAAGCTCT GCAGAACATC
    TCTGCTGATG ATCTTCCGGA TTCTGCCAGT CAGACGGCAC CACCTCAGGA CAAGACTTTC
    TCCTTCAGTG ATATTAAGAA GAAGCTACGC CTGGCACTTT GCTCTGCAGA TTCAGTTGTG
    TTTCCCATCA TTCCACCTGC AACAACACGC AACGGTCTTC CAGATCATGC AGATCCTGAA
    GACAACGAGA TTGTGTGCTT TCTGAAGGTG CAGCTGGCGG AGGCCATTAA CCTGCAGGAT
    AAGAACCAGA TGGCACAGAT CCAGGAGACC ACGCGCTGCG TCAGCCACCT CGACCCGCGC
    ACCTGCAGAA AACTGCTCAC TGCCATGGCA GAGGACTACA GGAAGCGGGC TCCATACATC
    GCCTATCTGA CACGTTGTCG GCAGGGCCTG CAGACATCTC AGGCTCATCT GGAGAGACTC
    CTGCAGAGAG TGTTGAGAGA TAAAGAAGTG GCCAACCGCT ACTTTACCAC TGTCTGTGTC
    CGACTACTGC TGGAGCACAT GGAGTCCAAG ATGCTTGACT TCATTAAAGC ATTTCAGGGA
    TGCACAGCAG CTGATGATAA GACAGCAGCC GTGGAGGATT TCCTGCGTTA CCTGTATGGA
    GCCATGGCCC ATGATGCCAT CTGGCAGTAC GCCAGTGAAG AGCAGCTCCA GGACGCACAG
    ATGGCCATCG AACGCAGCGT CATGAACCGC ATCTTCAAAC TGGCATTCTA CCCCAATCAG
    GATGGGGACA TCCACAGAGA CGAGCTATTC CATGAGCACA TTCAGCGACT GTCTAGAGTG
    GTGTCGGCCA ATCATAAAGC TCTGCAGATC CCTGAGGTGT ATCTGAAAGA GGCTCCGTGG
    CCTTCTGCAC AGGCTGAGAT CAAGACCATC AACGCCTATA AGACCCCGCG AGATAAAGTG
    CAGTGTATCT TGCGCATGTG CTCCACCATT ATGAACCTGC TCAGCCTGGC CAATGAGGAT
    GCTGTACCCG GCGCGGATGA CTTCGTCCCT GTGCTCGTCT TTGTTCTGAT TAAAGCAAAC
    CCACCCTGCC TTCTGTCCAC CATTCAGTAC ATCAATAATT TCTACGCCAG CAGGCTGAGC
    GGGGAGGAGT GCTATTGGTG GATGCAGTTC ACTGCTGCCG TGGAATTCAT TAAAACCATC
    GACGATCGCA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41043
    Clone ID Related Accession (Same CDS sequence) XM_009304093.3 , XM_009304093.2
    Accession Version XM_009304093.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304093.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGACAAC AGTTGGCAGC CATAACATGT TGGGAGCCCT CTGCCACCTC CCTGTCCGCT
    CATATACCCT TAGTAACCCC GTTTGCAGGC TCTCGCAGCC GAAGCCGATC CAACATGGCC
    CAGGAAGGAG AGACTGAAGC CAGTTCTCAA GAGTCTCTGC AAGAGGTCCC CGAGGAAGTG
    CTGGTCATCT CTCTAGGAAC ATCACCACAG ACCATTCCCG GGATGATGTC AGAAAACGAG
    GTTCTGACTT TGCATCTTGG AGATGGAGGA CAAGGTGACG CCCCTGTCGA CGACACCAAA
    TTACATGGCA AGCCTGACAA AACACTGCGC TTCTCACTTT GCAGTGACAA CCTGGAGGGC
    ATTTCTGAGG GGCCGTCTAA CCGCTCAAAT TCAGTTTCTT CTTTGGATTT GGAAGTGGAG
    TCCGTGTCAG AAGGAGGACC AGGACCGTCT GGTAGTAACG GTGCAGAAGC CTTACAGCTA
    CTGGAGCACG AACAAGCCAC TACACAGGAT AATTTGGACG ATAAACTGCG CAAGTTTGAG
    ATTCGGGACA TGATGGGCCT GACGGATGAC CGAGACATTT CTGAGACGGT CAGTGAGACT
    TGGAGCACTG ATGTCTTGGG GAGTGACTTT GACCCCAACA TGGATGAGGA CCGACTGCAG
    GAGATAGGGG CTGCAGCGGA GAATGCTCTG GGCAGCATGC TGTGTTTGCC TGTGCTTTTG
    GATCCATATG GTTCAACCAT CTCAGAGACC ACGAGCGAGG CTTGGAGTGT GGAGGTTCTG
    CCGAGTGACT CAGAAGCTGC AGACCTGAAG CAGGAGGAAC GTTTGCAGGA GCTGGAGAGC
    TGCTCCGGTG TGGGAAGCAC TTCAGACGAC ACAGAGGTCC GAGAGGTCAG CTCAAGACCA
    AGTACTCCAG GACTCAGCGT TGTTTCAGGC ATCAGTGCCA CGTCGGAGGA CATCCCTAAT
    AAGATTGAAG ACCTACGCTC GGAATGCAGC TCCGATTTTG GAGGCAAAGA CTCCGTGACC
    AGTCCAGATG GAGAGGACTC TGCTCATGGA GCTCACAGTC TCTCCTGTGC ACCTTCTCAA
    ACAGATTCAC TGCTGGCCAT GTTCGATCCC CTCTCCTCTG GAGAAGGTTC GTGCACTGGC
    ACCATAGTGC GACCCAAGGT ACACTATCCT CGTCCTCCTC ACCCTCCGCC TGACCCACCC
    ATTCCAGAGG CCAGCATAAC CAGCCACATG GAACCCCGTC CGCCCCTCTT TCCTCCCCAC
    CTGGCACAGG GTGACCTGGA GCACCCCAAG CAGAGGCACT CTTACCCTGA GAGGCTGGTG
    AGGAGCCGCA GCACAGATGT GGTGTCCGCT GGACGCAGAC CTAACAGTGA CCCCGGACTG
    AACCGCAGAA CCATGATGGA GGAGAGAGAC CCGGCTGGAG CTTACTCTAC TGGGCCCACC
    TCCTCTCCCA GCAAGGATTC CCTCAAAGGA GAGTCTGAGG ACAGGAAGGA CAGTGATGAT
    GAGAAGTCTG ATAGGAACAG ACCCTGGTGG AAGAAGCGCT TTGTTCCTGC CATTCCTAAA
    GTGCTCTATT GGACGGCAGA GGGTGAACTC CCAGTGCCGA TTGCCGGGCT TCGGAAGCGG
    GACAAGGCAG AAAAAGACGA GCAAGGAGCA GAGAGGGTCC CACAAGATGA GCCTTCACTG
    CTCAGACAGA GCTCCAAAAC CCAGTCAGCA GAGGCAATTA TGGACAAGTA CATCAGGTAC
    ATGGAGAAAC GACCCGCACA GGCTGACGGA GCAACTGCTG GAGGTTATGT TGGACAAGAG
    TCTTGTCGAG AAGGGGACAG TGAGCACGAC TCTCCTAAAG ATGAAGCTCT GCAGAACATC
    TCTGCTGATG ATCTTCCGGA TTCTGCCAGT CAGACGGCAC CACCTCAGGA CAAGACTTTC
    TCCTTCAGTG ATATTAAGAA GAAGCTACGC CTGGCACTTT GCTCTGCAGA TTCAGTTGTG
    TTTCCCATCA TTCCACCTGC AACAACACGC AACGGTCTTC CAGATCATGC AGATCCTGAA
    GACAACGAGA TTGTGTGCTT TCTGAAGGTG CAGCTGGCGG AGGCCATTAA CCTGCAGGAT
    AAGAACCAGA TGGCACAGAT CCAGGAGACC ACGCGCTGCG TCAGCCACCT CGACCCGCGC
    ACCTGCAGAA AACTGCTCAC TGCCATGGCA GAGGACTACA GGAAGCGGGC TCCATACATC
    GCCTATCTGA CACGTTGTCG GCAGGGCCTG CAGACATCTC AGGCTCATCT GGAGAGACTC
    CTGCAGAGAG TGTTGAGAGA TAAAGAAGTG GCCAACCGCT ACTTTACCAC TGTCTGTGTC
    CGACTACTGC TGGAGCACAT GGAGTCCAAG ATGCTTGACT TCATTAAAGC ATTTCAGGGA
    TGCACAGCAG CTGATGATAA GACAGCAGCC GTGGAGGATT TCCTGCGTTA CCTGTATGGA
    GCCATGGCCC ATGATGCCAT CTGGCAGTAC GCCAGTGAAG AGCAGCTCCA GGACGCACAG
    ATGGCCATCG AACGCAGCGT CATGAACCGC ATCTTCAAAC TGGCATTCTA CCCCAATCAG
    GATGGGGACA TCCACAGAGA CGAGCTATTC CATGAGCACA TTCAGCGACT GTCTAGAGTG
    GTGTCGGCCA ATCATAAAGC TCTGCAGATC CCTGAGGTGT ATCTGAAAGA GGCTCCGTGG
    CCTTCTGCAC AGGCTGAGAT CAAGACCATC AACGCCTATA AGACCCCGCG AGATAAAGTG
    CAGTGTATCT TGCGCATGTG CTCCACCATT ATGAACCTGC TCAGCCTGGC CAATGAGGAT
    GCTGTACCCG GCGCGGATGA CTTCGTCCCT GTGCTCGTCT TTGTTCTGAT TAAAGCAAAC
    CCACCCTGCC TTCTGTCCAC CATTCAGTAC ATCAATAATT TCTACGCCAG CAGGCTGAGC
    GGGGAGGAGT GCTATTGGTG GATGCAGTTC ACTGCTGCCG TGGAATTCAT TAAAACCATC
    GACGATCGCA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302368.1
    CDS200..4654
    Translation

    Target ORF information:

    RefSeq Version XM_009304093.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304093.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGACAAC AGTTGGCAGC CATAACATGT TGGGAGCCCT CTGCCACCTC CCTGTCCGCT
    CATATACCCT TAGTAACCCC GTTTGCAGGC TCTCGCAGCC GAAGCCGATC CAACATGGCC
    CAGGAAGGAG AGACTGAAGC CAGTTCTCAA GAGTCTCTGC AAGAGGTCCC CGAGGAAGTG
    CTGGTCATCT CTCTAGGAAC ATCACCACAG ACCATTCCCG GGATGATGTC AGAAAACGAG
    GTTCTGACTT TGCATCTTGG AGATGGAGGA CAAGGTGACG CCCCTGTCGA CGACACCAAA
    TTACATGGCA AGCCTGACAA AACACTGCGC TTCTCACTTT GCAGTGACAA CCTGGAGGGC
    ATTTCTGAGG GGCCGTCTAA CCGCTCAAAT TCAGTTTCTT CTTTGGATTT GGAAGTGGAG
    TCCGTGTCAG AAGGAGGACC AGGACCGTCT GGTAGTAACG GTGCAGAAGC CTTACAGCTA
    CTGGAGCACG AACAAGCCAC TACACAGGAT AATTTGGACG ATAAACTGCG CAAGTTTGAG
    ATTCGGGACA TGATGGGCCT GACGGATGAC CGAGACATTT CTGAGACGGT CAGTGAGACT
    TGGAGCACTG ATGTCTTGGG GAGTGACTTT GACCCCAACA TGGATGAGGA CCGACTGCAG
    GAGATAGGGG CTGCAGCGGA GAATGCTCTG GGCAGCATGC TGTGTTTGCC TGTGCTTTTG
    GATCCATATG GTTCAACCAT CTCAGAGACC ACGAGCGAGG CTTGGAGTGT GGAGGTTCTG
    CCGAGTGACT CAGAAGCTGC AGACCTGAAG CAGGAGGAAC GTTTGCAGGA GCTGGAGAGC
    TGCTCCGGTG TGGGAAGCAC TTCAGACGAC ACAGAGGTCC GAGAGGTCAG CTCAAGACCA
    AGTACTCCAG GACTCAGCGT TGTTTCAGGC ATCAGTGCCA CGTCGGAGGA CATCCCTAAT
    AAGATTGAAG ACCTACGCTC GGAATGCAGC TCCGATTTTG GAGGCAAAGA CTCCGTGACC
    AGTCCAGATG GAGAGGACTC TGCTCATGGA GCTCACAGTC TCTCCTGTGC ACCTTCTCAA
    ACAGATTCAC TGCTGGCCAT GTTCGATCCC CTCTCCTCTG GAGAAGGTTC GTGCACTGGC
    ACCATAGTGC GACCCAAGGT ACACTATCCT CGTCCTCCTC ACCCTCCGCC TGACCCACCC
    ATTCCAGAGG CCAGCATAAC CAGCCACATG GAACCCCGTC CGCCCCTCTT TCCTCCCCAC
    CTGGCACAGG GTGACCTGGA GCACCCCAAG CAGAGGCACT CTTACCCTGA GAGGCTGGTG
    AGGAGCCGCA GCACAGATGT GGTGTCCGCT GGACGCAGAC CTAACAGTGA CCCCGGACTG
    AACCGCAGAA CCATGATGGA GGAGAGAGAC CCGGCTGGAG CTTACTCTAC TGGGCCCACC
    TCCTCTCCCA GCAAGGATTC CCTCAAAGGA GAGTCTGAGG ACAGGAAGGA CAGTGATGAT
    GAGAAGTCTG ATAGGAACAG ACCCTGGTGG AAGAAGCGCT TTGTTCCTGC CATTCCTAAA
    GTGCTCTATT GGACGGCAGA GGGTGAACTC CCAGTGCCGA TTGCCGGGCT TCGGAAGCGG
    GACAAGGCAG AAAAAGACGA GCAAGGAGCA GAGAGGGTCC CACAAGATGA GCCTTCACTG
    CTCAGACAGA GCTCCAAAAC CCAGTCAGCA GAGGCAATTA TGGACAAGTA CATCAGGTAC
    ATGGAGAAAC GACCCGCACA GGCTGACGGA GCAACTGCTG GAGGTTATGT TGGACAAGAG
    TCTTGTCGAG AAGGGGACAG TGAGCACGAC TCTCCTAAAG ATGAAGCTCT GCAGAACATC
    TCTGCTGATG ATCTTCCGGA TTCTGCCAGT CAGACGGCAC CACCTCAGGA CAAGACTTTC
    TCCTTCAGTG ATATTAAGAA GAAGCTACGC CTGGCACTTT GCTCTGCAGA TTCAGTTGTG
    TTTCCCATCA TTCCACCTGC AACAACACGC AACGGTCTTC CAGATCATGC AGATCCTGAA
    GACAACGAGA TTGTGTGCTT TCTGAAGGTG CAGCTGGCGG AGGCCATTAA CCTGCAGGAT
    AAGAACCAGA TGGCACAGAT CCAGGAGACC ACGCGCTGCG TCAGCCACCT CGACCCGCGC
    ACCTGCAGAA AACTGCTCAC TGCCATGGCA GAGGACTACA GGAAGCGGGC TCCATACATC
    GCCTATCTGA CACGTTGTCG GCAGGGCCTG CAGACATCTC AGGCTCATCT GGAGAGACTC
    CTGCAGAGAG TGTTGAGAGA TAAAGAAGTG GCCAACCGCT ACTTTACCAC TGTCTGTGTC
    CGACTACTGC TGGAGCACAT GGAGTCCAAG ATGCTTGACT TCATTAAAGC ATTTCAGGGA
    TGCACAGCAG CTGATGATAA GACAGCAGCC GTGGAGGATT TCCTGCGTTA CCTGTATGGA
    GCCATGGCCC ATGATGCCAT CTGGCAGTAC GCCAGTGAAG AGCAGCTCCA GGACGCACAG
    ATGGCCATCG AACGCAGCGT CATGAACCGC ATCTTCAAAC TGGCATTCTA CCCCAATCAG
    GATGGGGACA TCCACAGAGA CGAGCTATTC CATGAGCACA TTCAGCGACT GTCTAGAGTG
    GTGTCGGCCA ATCATAAAGC TCTGCAGATC CCTGAGGTGT ATCTGAAAGA GGCTCCGTGG
    CCTTCTGCAC AGGCTGAGAT CAAGACCATC AACGCCTATA AGACCCCGCG AGATAAAGTG
    CAGTGTATCT TGCGCATGTG CTCCACCATT ATGAACCTGC TCAGCCTGGC CAATGAGGAT
    GCTGTACCCG GCGCGGATGA CTTCGTCCCT GTGCTCGTCT TTGTTCTGAT TAAAGCAAAC
    CCACCCTGCC TTCTGTCCAC CATTCAGTAC ATCAATAATT TCTACGCCAG CAGGCTGAGC
    GGGGAGGAGT GCTATTGGTG GATGCAGTTC ACTGCTGCCG TGGAATTCAT TAAAACCATC
    GACGATCGCA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41044
    Clone ID Related Accession (Same CDS sequence) XM_009304094.2 , XM_009304094.3
    Accession Version XM_009304094.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4452bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304094.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGACAAC AGTTGGCAGC CATAACATGT TGGGAGCCCT CTGCCACCTC CCTGTCCGCT
    CATATACCCT TAGTAACCCC GTTTGGCTCT CGCAGCCGAA GCCGATCCAA CATGGCCCAG
    GAAGGAGAGA CTGAAGCCAG TTCTCAAGAG TCTCTGCAAG AGGTCCCCGA GGAAGTGCTG
    GTCATCTCTC TAGGAACATC ACCACAGACC ATTCCCGGGA TGATGTCAGA AAACGAGGTT
    CTGACTTTGC ATCTTGGAGA TGGAGGACAA GGTGACGCCC CTGTCGACGA CACCAAATTA
    CATGGCAAGC CTGACAAAAC ACTGCGCTTC TCACTTTGCA GTGACAACCT GGAGGGCATT
    TCTGAGGGGC CGTCTAACCG CTCAAATTCA GTTTCTTCTT TGGATTTGGA AGTGGAGTCC
    GTGTCAGAAG GAGGACCAGG ACCGTCTGGT AGTAACGGTG CAGAAGCCTT ACAGCTACTG
    GAGCACGAAC AAGCCACTAC ACAGGATAAT TTGGACGATA AACTGCGCAA GTTTGAGATT
    CGGGACATGA TGGGCCTGAC GGATGACCGA GACATTTCTG AGACGGTCAG TGAGACTTGG
    AGCACTGATG TCTTGGGGAG TGACTTTGAC CCCAACATGG ATGAGGACCG ACTGCAGGAG
    ATAGGGGCTG CAGCGGAGAA TGCTCTGGGC AGCATGCTGT GTTTGCCTGT GCTTTTGGAT
    CCATATGGTT CAACCATCTC AGAGACCACG AGCGAGGCTT GGAGTGTGGA GGTTCTGCCG
    AGTGACTCAG AAGCTGCAGA CCTGAAGCAG GAGGAACGTT TGCAGGAGCT GGAGAGCTGC
    TCCGGTGTGG GAAGCACTTC AGACGACACA GAGGTCCGAG AGGTCAGCTC AAGACCAAGT
    ACTCCAGGAC TCAGCGTTGT TTCAGGCATC AGTGCCACGT CGGAGGACAT CCCTAATAAG
    ATTGAAGACC TACGCTCGGA ATGCAGCTCC GATTTTGGAG GCAAAGACTC CGTGACCAGT
    CCAGATGGAG AGGACTCTGC TCATGGAGCT CACAGTCTCT CCTGTGCACC TTCTCAAACA
    GATTCACTGC TGGCCATGTT CGATCCCCTC TCCTCTGGAG AAGGTTCGTG CACTGGCACC
    ATAGTGCGAC CCAAGGTACA CTATCCTCGT CCTCCTCACC CTCCGCCTGA CCCACCCATT
    CCAGAGGCCA GCATAACCAG CCACATGGAA CCCCGTCCGC CCCTCTTTCC TCCCCACCTG
    GCACAGGGTG ACCTGGAGCA CCCCAAGCAG AGGCACTCTT ACCCTGAGAG GCTGGTGAGG
    AGCCGCAGCA CAGATGTGGT GTCCGCTGGA CGCAGACCTA ACAGTGACCC CGGACTGAAC
    CGCAGAACCA TGATGGAGGA GAGAGACCCG GCTGGAGCTT ACTCTACTGG GCCCACCTCC
    TCTCCCAGCA AGGATTCCCT CAAAGGAGAG TCTGAGGACA GGAAGGACAG TGATGATGAG
    AAGTCTGATA GGAACAGACC CTGGTGGAAG AAGCGCTTTG TTCCTGCCAT TCCTAAAGTG
    CTCTATTGGA CGGCAGAGGG TGAACTCCCA GTGCCGATTG CCGGGCTTCG GAAGCGGGAC
    AAGGCAGAAA AAGACGAGCA AGGAGCAGAG AGGGTCCCAC AAGATGAGCC TTCACTGCTC
    AGACAGAGCT CCAAAACCCA GTCAGCAGAG GCAATTATGG ACAAGTACAT CAGGTACATG
    GAGAAACGAC CCGCACAGGC TGACGGAGCA ACTGCTGGAG GTTATGTTGG ACAAGAGTCT
    TGTCGAGAAG GGGACAGTGA GCACGACTCT CCTAAAGATG AAGCTCTGCA GAACATCTCT
    GCTGATGATC TTCCGGATTC TGCCAGTCAG ACGGCACCAC CTCAGGACAA GACTTTCTCC
    TTCAGTGATA TTAAGAAGAA GCTACGCCTG GCACTTTGCT CTGCAGATTC AGTTGTGTTT
    CCCATCATTC CACCTGCAAC AACACGCAAC GGTCTTCCAG ATCATGCAGA TCCTGAAGAC
    AACGAGATTG TGTGCTTTCT GAAGGTGCAG CTGGCGGAGG CCATTAACCT GCAGGATAAG
    AACCAGATGG CACAGATCCA GGAGACCACG CGCTGCGTCA GCCACCTCGA CCCGCGCACC
    TGCAGAAAAC TGCTCACTGC CATGGCAGAG GACTACAGGA AGCGGGCTCC ATACATCGCC
    TATCTGACAC GTTGTCGGCA GGGCCTGCAG ACATCTCAGG CTCATCTGGA GAGACTCCTG
    CAGAGAGTGT TGAGAGATAA AGAAGTGGCC AACCGCTACT TTACCACTGT CTGTGTCCGA
    CTACTGCTGG AGCACATGGA GTCCAAGATG CTTGACTTCA TTAAAGCATT TCAGGGATGC
    ACAGCAGCTG ATGATAAGAC AGCAGCCGTG GAGGATTTCC TGCGTTACCT GTATGGAGCC
    ATGGCCCATG ATGCCATCTG GCAGTACGCC AGTGAAGAGC AGCTCCAGGA CGCACAGATG
    GCCATCGAAC GCAGCGTCAT GAACCGCATC TTCAAACTGG CATTCTACCC CAATCAGGAT
    GGGGACATCC ACAGAGACGA GCTATTCCAT GAGCACATTC AGCGACTGTC TAGAGTGGTG
    TCGGCCAATC ATAAAGCTCT GCAGATCCCT GAGGTGTATC TGAAAGAGGC TCCGTGGCCT
    TCTGCACAGG CTGAGATCAA GACCATCAAC GCCTATAAGA CCCCGCGAGA TAAAGTGCAG
    TGTATCTTGC GCATGTGCTC CACCATTATG AACCTGCTCA GCCTGGCCAA TGAGGATGCT
    GTACCCGGCG CGGATGACTT CGTCCCTGTG CTCGTCTTTG TTCTGATTAA AGCAAACCCA
    CCCTGCCTTC TGTCCACCAT TCAGTACATC AATAATTTCT ACGCCAGCAG GCTGAGCGGG
    GAGGAGTGCT ATTGGTGGAT GCAGTTCACT GCTGCCGTGG AATTCATTAA AACCATCGAC
    GATCGCAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302369.1
    CDS200..4651
    Translation

    Target ORF information:

    RefSeq Version XM_009304094.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304094.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGACAAC AGTTGGCAGC CATAACATGT TGGGAGCCCT CTGCCACCTC CCTGTCCGCT
    CATATACCCT TAGTAACCCC GTTTGGCTCT CGCAGCCGAA GCCGATCCAA CATGGCCCAG
    GAAGGAGAGA CTGAAGCCAG TTCTCAAGAG TCTCTGCAAG AGGTCCCCGA GGAAGTGCTG
    GTCATCTCTC TAGGAACATC ACCACAGACC ATTCCCGGGA TGATGTCAGA AAACGAGGTT
    CTGACTTTGC ATCTTGGAGA TGGAGGACAA GGTGACGCCC CTGTCGACGA CACCAAATTA
    CATGGCAAGC CTGACAAAAC ACTGCGCTTC TCACTTTGCA GTGACAACCT GGAGGGCATT
    TCTGAGGGGC CGTCTAACCG CTCAAATTCA GTTTCTTCTT TGGATTTGGA AGTGGAGTCC
    GTGTCAGAAG GAGGACCAGG ACCGTCTGGT AGTAACGGTG CAGAAGCCTT ACAGCTACTG
    GAGCACGAAC AAGCCACTAC ACAGGATAAT TTGGACGATA AACTGCGCAA GTTTGAGATT
    CGGGACATGA TGGGCCTGAC GGATGACCGA GACATTTCTG AGACGGTCAG TGAGACTTGG
    AGCACTGATG TCTTGGGGAG TGACTTTGAC CCCAACATGG ATGAGGACCG ACTGCAGGAG
    ATAGGGGCTG CAGCGGAGAA TGCTCTGGGC AGCATGCTGT GTTTGCCTGT GCTTTTGGAT
    CCATATGGTT CAACCATCTC AGAGACCACG AGCGAGGCTT GGAGTGTGGA GGTTCTGCCG
    AGTGACTCAG AAGCTGCAGA CCTGAAGCAG GAGGAACGTT TGCAGGAGCT GGAGAGCTGC
    TCCGGTGTGG GAAGCACTTC AGACGACACA GAGGTCCGAG AGGTCAGCTC AAGACCAAGT
    ACTCCAGGAC TCAGCGTTGT TTCAGGCATC AGTGCCACGT CGGAGGACAT CCCTAATAAG
    ATTGAAGACC TACGCTCGGA ATGCAGCTCC GATTTTGGAG GCAAAGACTC CGTGACCAGT
    CCAGATGGAG AGGACTCTGC TCATGGAGCT CACAGTCTCT CCTGTGCACC TTCTCAAACA
    GATTCACTGC TGGCCATGTT CGATCCCCTC TCCTCTGGAG AAGGTTCGTG CACTGGCACC
    ATAGTGCGAC CCAAGGTACA CTATCCTCGT CCTCCTCACC CTCCGCCTGA CCCACCCATT
    CCAGAGGCCA GCATAACCAG CCACATGGAA CCCCGTCCGC CCCTCTTTCC TCCCCACCTG
    GCACAGGGTG ACCTGGAGCA CCCCAAGCAG AGGCACTCTT ACCCTGAGAG GCTGGTGAGG
    AGCCGCAGCA CAGATGTGGT GTCCGCTGGA CGCAGACCTA ACAGTGACCC CGGACTGAAC
    CGCAGAACCA TGATGGAGGA GAGAGACCCG GCTGGAGCTT ACTCTACTGG GCCCACCTCC
    TCTCCCAGCA AGGATTCCCT CAAAGGAGAG TCTGAGGACA GGAAGGACAG TGATGATGAG
    AAGTCTGATA GGAACAGACC CTGGTGGAAG AAGCGCTTTG TTCCTGCCAT TCCTAAAGTG
    CTCTATTGGA CGGCAGAGGG TGAACTCCCA GTGCCGATTG CCGGGCTTCG GAAGCGGGAC
    AAGGCAGAAA AAGACGAGCA AGGAGCAGAG AGGGTCCCAC AAGATGAGCC TTCACTGCTC
    AGACAGAGCT CCAAAACCCA GTCAGCAGAG GCAATTATGG ACAAGTACAT CAGGTACATG
    GAGAAACGAC CCGCACAGGC TGACGGAGCA ACTGCTGGAG GTTATGTTGG ACAAGAGTCT
    TGTCGAGAAG GGGACAGTGA GCACGACTCT CCTAAAGATG AAGCTCTGCA GAACATCTCT
    GCTGATGATC TTCCGGATTC TGCCAGTCAG ACGGCACCAC CTCAGGACAA GACTTTCTCC
    TTCAGTGATA TTAAGAAGAA GCTACGCCTG GCACTTTGCT CTGCAGATTC AGTTGTGTTT
    CCCATCATTC CACCTGCAAC AACACGCAAC GGTCTTCCAG ATCATGCAGA TCCTGAAGAC
    AACGAGATTG TGTGCTTTCT GAAGGTGCAG CTGGCGGAGG CCATTAACCT GCAGGATAAG
    AACCAGATGG CACAGATCCA GGAGACCACG CGCTGCGTCA GCCACCTCGA CCCGCGCACC
    TGCAGAAAAC TGCTCACTGC CATGGCAGAG GACTACAGGA AGCGGGCTCC ATACATCGCC
    TATCTGACAC GTTGTCGGCA GGGCCTGCAG ACATCTCAGG CTCATCTGGA GAGACTCCTG
    CAGAGAGTGT TGAGAGATAA AGAAGTGGCC AACCGCTACT TTACCACTGT CTGTGTCCGA
    CTACTGCTGG AGCACATGGA GTCCAAGATG CTTGACTTCA TTAAAGCATT TCAGGGATGC
    ACAGCAGCTG ATGATAAGAC AGCAGCCGTG GAGGATTTCC TGCGTTACCT GTATGGAGCC
    ATGGCCCATG ATGCCATCTG GCAGTACGCC AGTGAAGAGC AGCTCCAGGA CGCACAGATG
    GCCATCGAAC GCAGCGTCAT GAACCGCATC TTCAAACTGG CATTCTACCC CAATCAGGAT
    GGGGACATCC ACAGAGACGA GCTATTCCAT GAGCACATTC AGCGACTGTC TAGAGTGGTG
    TCGGCCAATC ATAAAGCTCT GCAGATCCCT GAGGTGTATC TGAAAGAGGC TCCGTGGCCT
    TCTGCACAGG CTGAGATCAA GACCATCAAC GCCTATAAGA CCCCGCGAGA TAAAGTGCAG
    TGTATCTTGC GCATGTGCTC CACCATTATG AACCTGCTCA GCCTGGCCAA TGAGGATGCT
    GTACCCGGCG CGGATGACTT CGTCCCTGTG CTCGTCTTTG TTCTGATTAA AGCAAACCCA
    CCCTGCCTTC TGTCCACCAT TCAGTACATC AATAATTTCT ACGCCAGCAG GCTGAGCGGG
    GAGGAGTGCT ATTGGTGGAT GCAGTTCACT GCTGCCGTGG AATTCATTAA AACCATCGAC
    GATCGCAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41044
    Clone ID Related Accession (Same CDS sequence) XM_009304094.2 , XM_009304094.3
    Accession Version XM_009304094.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4452bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304094.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGACAAC AGTTGGCAGC CATAACATGT TGGGAGCCCT CTGCCACCTC CCTGTCCGCT
    CATATACCCT TAGTAACCCC GTTTGGCTCT CGCAGCCGAA GCCGATCCAA CATGGCCCAG
    GAAGGAGAGA CTGAAGCCAG TTCTCAAGAG TCTCTGCAAG AGGTCCCCGA GGAAGTGCTG
    GTCATCTCTC TAGGAACATC ACCACAGACC ATTCCCGGGA TGATGTCAGA AAACGAGGTT
    CTGACTTTGC ATCTTGGAGA TGGAGGACAA GGTGACGCCC CTGTCGACGA CACCAAATTA
    CATGGCAAGC CTGACAAAAC ACTGCGCTTC TCACTTTGCA GTGACAACCT GGAGGGCATT
    TCTGAGGGGC CGTCTAACCG CTCAAATTCA GTTTCTTCTT TGGATTTGGA AGTGGAGTCC
    GTGTCAGAAG GAGGACCAGG ACCGTCTGGT AGTAACGGTG CAGAAGCCTT ACAGCTACTG
    GAGCACGAAC AAGCCACTAC ACAGGATAAT TTGGACGATA AACTGCGCAA GTTTGAGATT
    CGGGACATGA TGGGCCTGAC GGATGACCGA GACATTTCTG AGACGGTCAG TGAGACTTGG
    AGCACTGATG TCTTGGGGAG TGACTTTGAC CCCAACATGG ATGAGGACCG ACTGCAGGAG
    ATAGGGGCTG CAGCGGAGAA TGCTCTGGGC AGCATGCTGT GTTTGCCTGT GCTTTTGGAT
    CCATATGGTT CAACCATCTC AGAGACCACG AGCGAGGCTT GGAGTGTGGA GGTTCTGCCG
    AGTGACTCAG AAGCTGCAGA CCTGAAGCAG GAGGAACGTT TGCAGGAGCT GGAGAGCTGC
    TCCGGTGTGG GAAGCACTTC AGACGACACA GAGGTCCGAG AGGTCAGCTC AAGACCAAGT
    ACTCCAGGAC TCAGCGTTGT TTCAGGCATC AGTGCCACGT CGGAGGACAT CCCTAATAAG
    ATTGAAGACC TACGCTCGGA ATGCAGCTCC GATTTTGGAG GCAAAGACTC CGTGACCAGT
    CCAGATGGAG AGGACTCTGC TCATGGAGCT CACAGTCTCT CCTGTGCACC TTCTCAAACA
    GATTCACTGC TGGCCATGTT CGATCCCCTC TCCTCTGGAG AAGGTTCGTG CACTGGCACC
    ATAGTGCGAC CCAAGGTACA CTATCCTCGT CCTCCTCACC CTCCGCCTGA CCCACCCATT
    CCAGAGGCCA GCATAACCAG CCACATGGAA CCCCGTCCGC CCCTCTTTCC TCCCCACCTG
    GCACAGGGTG ACCTGGAGCA CCCCAAGCAG AGGCACTCTT ACCCTGAGAG GCTGGTGAGG
    AGCCGCAGCA CAGATGTGGT GTCCGCTGGA CGCAGACCTA ACAGTGACCC CGGACTGAAC
    CGCAGAACCA TGATGGAGGA GAGAGACCCG GCTGGAGCTT ACTCTACTGG GCCCACCTCC
    TCTCCCAGCA AGGATTCCCT CAAAGGAGAG TCTGAGGACA GGAAGGACAG TGATGATGAG
    AAGTCTGATA GGAACAGACC CTGGTGGAAG AAGCGCTTTG TTCCTGCCAT TCCTAAAGTG
    CTCTATTGGA CGGCAGAGGG TGAACTCCCA GTGCCGATTG CCGGGCTTCG GAAGCGGGAC
    AAGGCAGAAA AAGACGAGCA AGGAGCAGAG AGGGTCCCAC AAGATGAGCC TTCACTGCTC
    AGACAGAGCT CCAAAACCCA GTCAGCAGAG GCAATTATGG ACAAGTACAT CAGGTACATG
    GAGAAACGAC CCGCACAGGC TGACGGAGCA ACTGCTGGAG GTTATGTTGG ACAAGAGTCT
    TGTCGAGAAG GGGACAGTGA GCACGACTCT CCTAAAGATG AAGCTCTGCA GAACATCTCT
    GCTGATGATC TTCCGGATTC TGCCAGTCAG ACGGCACCAC CTCAGGACAA GACTTTCTCC
    TTCAGTGATA TTAAGAAGAA GCTACGCCTG GCACTTTGCT CTGCAGATTC AGTTGTGTTT
    CCCATCATTC CACCTGCAAC AACACGCAAC GGTCTTCCAG ATCATGCAGA TCCTGAAGAC
    AACGAGATTG TGTGCTTTCT GAAGGTGCAG CTGGCGGAGG CCATTAACCT GCAGGATAAG
    AACCAGATGG CACAGATCCA GGAGACCACG CGCTGCGTCA GCCACCTCGA CCCGCGCACC
    TGCAGAAAAC TGCTCACTGC CATGGCAGAG GACTACAGGA AGCGGGCTCC ATACATCGCC
    TATCTGACAC GTTGTCGGCA GGGCCTGCAG ACATCTCAGG CTCATCTGGA GAGACTCCTG
    CAGAGAGTGT TGAGAGATAA AGAAGTGGCC AACCGCTACT TTACCACTGT CTGTGTCCGA
    CTACTGCTGG AGCACATGGA GTCCAAGATG CTTGACTTCA TTAAAGCATT TCAGGGATGC
    ACAGCAGCTG ATGATAAGAC AGCAGCCGTG GAGGATTTCC TGCGTTACCT GTATGGAGCC
    ATGGCCCATG ATGCCATCTG GCAGTACGCC AGTGAAGAGC AGCTCCAGGA CGCACAGATG
    GCCATCGAAC GCAGCGTCAT GAACCGCATC TTCAAACTGG CATTCTACCC CAATCAGGAT
    GGGGACATCC ACAGAGACGA GCTATTCCAT GAGCACATTC AGCGACTGTC TAGAGTGGTG
    TCGGCCAATC ATAAAGCTCT GCAGATCCCT GAGGTGTATC TGAAAGAGGC TCCGTGGCCT
    TCTGCACAGG CTGAGATCAA GACCATCAAC GCCTATAAGA CCCCGCGAGA TAAAGTGCAG
    TGTATCTTGC GCATGTGCTC CACCATTATG AACCTGCTCA GCCTGGCCAA TGAGGATGCT
    GTACCCGGCG CGGATGACTT CGTCCCTGTG CTCGTCTTTG TTCTGATTAA AGCAAACCCA
    CCCTGCCTTC TGTCCACCAT TCAGTACATC AATAATTTCT ACGCCAGCAG GCTGAGCGGG
    GAGGAGTGCT ATTGGTGGAT GCAGTTCACT GCTGCCGTGG AATTCATTAA AACCATCGAC
    GATCGCAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302369.1
    CDS202..4653
    Translation

    Target ORF information:

    RefSeq Version XM_009304094.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304094.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGACAAC AGTTGGCAGC CATAACATGT TGGGAGCCCT CTGCCACCTC CCTGTCCGCT
    CATATACCCT TAGTAACCCC GTTTGGCTCT CGCAGCCGAA GCCGATCCAA CATGGCCCAG
    GAAGGAGAGA CTGAAGCCAG TTCTCAAGAG TCTCTGCAAG AGGTCCCCGA GGAAGTGCTG
    GTCATCTCTC TAGGAACATC ACCACAGACC ATTCCCGGGA TGATGTCAGA AAACGAGGTT
    CTGACTTTGC ATCTTGGAGA TGGAGGACAA GGTGACGCCC CTGTCGACGA CACCAAATTA
    CATGGCAAGC CTGACAAAAC ACTGCGCTTC TCACTTTGCA GTGACAACCT GGAGGGCATT
    TCTGAGGGGC CGTCTAACCG CTCAAATTCA GTTTCTTCTT TGGATTTGGA AGTGGAGTCC
    GTGTCAGAAG GAGGACCAGG ACCGTCTGGT AGTAACGGTG CAGAAGCCTT ACAGCTACTG
    GAGCACGAAC AAGCCACTAC ACAGGATAAT TTGGACGATA AACTGCGCAA GTTTGAGATT
    CGGGACATGA TGGGCCTGAC GGATGACCGA GACATTTCTG AGACGGTCAG TGAGACTTGG
    AGCACTGATG TCTTGGGGAG TGACTTTGAC CCCAACATGG ATGAGGACCG ACTGCAGGAG
    ATAGGGGCTG CAGCGGAGAA TGCTCTGGGC AGCATGCTGT GTTTGCCTGT GCTTTTGGAT
    CCATATGGTT CAACCATCTC AGAGACCACG AGCGAGGCTT GGAGTGTGGA GGTTCTGCCG
    AGTGACTCAG AAGCTGCAGA CCTGAAGCAG GAGGAACGTT TGCAGGAGCT GGAGAGCTGC
    TCCGGTGTGG GAAGCACTTC AGACGACACA GAGGTCCGAG AGGTCAGCTC AAGACCAAGT
    ACTCCAGGAC TCAGCGTTGT TTCAGGCATC AGTGCCACGT CGGAGGACAT CCCTAATAAG
    ATTGAAGACC TACGCTCGGA ATGCAGCTCC GATTTTGGAG GCAAAGACTC CGTGACCAGT
    CCAGATGGAG AGGACTCTGC TCATGGAGCT CACAGTCTCT CCTGTGCACC TTCTCAAACA
    GATTCACTGC TGGCCATGTT CGATCCCCTC TCCTCTGGAG AAGGTTCGTG CACTGGCACC
    ATAGTGCGAC CCAAGGTACA CTATCCTCGT CCTCCTCACC CTCCGCCTGA CCCACCCATT
    CCAGAGGCCA GCATAACCAG CCACATGGAA CCCCGTCCGC CCCTCTTTCC TCCCCACCTG
    GCACAGGGTG ACCTGGAGCA CCCCAAGCAG AGGCACTCTT ACCCTGAGAG GCTGGTGAGG
    AGCCGCAGCA CAGATGTGGT GTCCGCTGGA CGCAGACCTA ACAGTGACCC CGGACTGAAC
    CGCAGAACCA TGATGGAGGA GAGAGACCCG GCTGGAGCTT ACTCTACTGG GCCCACCTCC
    TCTCCCAGCA AGGATTCCCT CAAAGGAGAG TCTGAGGACA GGAAGGACAG TGATGATGAG
    AAGTCTGATA GGAACAGACC CTGGTGGAAG AAGCGCTTTG TTCCTGCCAT TCCTAAAGTG
    CTCTATTGGA CGGCAGAGGG TGAACTCCCA GTGCCGATTG CCGGGCTTCG GAAGCGGGAC
    AAGGCAGAAA AAGACGAGCA AGGAGCAGAG AGGGTCCCAC AAGATGAGCC TTCACTGCTC
    AGACAGAGCT CCAAAACCCA GTCAGCAGAG GCAATTATGG ACAAGTACAT CAGGTACATG
    GAGAAACGAC CCGCACAGGC TGACGGAGCA ACTGCTGGAG GTTATGTTGG ACAAGAGTCT
    TGTCGAGAAG GGGACAGTGA GCACGACTCT CCTAAAGATG AAGCTCTGCA GAACATCTCT
    GCTGATGATC TTCCGGATTC TGCCAGTCAG ACGGCACCAC CTCAGGACAA GACTTTCTCC
    TTCAGTGATA TTAAGAAGAA GCTACGCCTG GCACTTTGCT CTGCAGATTC AGTTGTGTTT
    CCCATCATTC CACCTGCAAC AACACGCAAC GGTCTTCCAG ATCATGCAGA TCCTGAAGAC
    AACGAGATTG TGTGCTTTCT GAAGGTGCAG CTGGCGGAGG CCATTAACCT GCAGGATAAG
    AACCAGATGG CACAGATCCA GGAGACCACG CGCTGCGTCA GCCACCTCGA CCCGCGCACC
    TGCAGAAAAC TGCTCACTGC CATGGCAGAG GACTACAGGA AGCGGGCTCC ATACATCGCC
    TATCTGACAC GTTGTCGGCA GGGCCTGCAG ACATCTCAGG CTCATCTGGA GAGACTCCTG
    CAGAGAGTGT TGAGAGATAA AGAAGTGGCC AACCGCTACT TTACCACTGT CTGTGTCCGA
    CTACTGCTGG AGCACATGGA GTCCAAGATG CTTGACTTCA TTAAAGCATT TCAGGGATGC
    ACAGCAGCTG ATGATAAGAC AGCAGCCGTG GAGGATTTCC TGCGTTACCT GTATGGAGCC
    ATGGCCCATG ATGCCATCTG GCAGTACGCC AGTGAAGAGC AGCTCCAGGA CGCACAGATG
    GCCATCGAAC GCAGCGTCAT GAACCGCATC TTCAAACTGG CATTCTACCC CAATCAGGAT
    GGGGACATCC ACAGAGACGA GCTATTCCAT GAGCACATTC AGCGACTGTC TAGAGTGGTG
    TCGGCCAATC ATAAAGCTCT GCAGATCCCT GAGGTGTATC TGAAAGAGGC TCCGTGGCCT
    TCTGCACAGG CTGAGATCAA GACCATCAAC GCCTATAAGA CCCCGCGAGA TAAAGTGCAG
    TGTATCTTGC GCATGTGCTC CACCATTATG AACCTGCTCA GCCTGGCCAA TGAGGATGCT
    GTACCCGGCG CGGATGACTT CGTCCCTGTG CTCGTCTTTG TTCTGATTAA AGCAAACCCA
    CCCTGCCTTC TGTCCACCAT TCAGTACATC AATAATTTCT ACGCCAGCAG GCTGAGCGGG
    GAGGAGTGCT ATTGGTGGAT GCAGTTCACT GCTGCCGTGG AATTCATTAA AACCATCGAC
    GATCGCAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41045
    Clone ID Related Accession (Same CDS sequence) XM_009304095.3 , XM_009304095.2
    Accession Version XM_009304095.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304095.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGCAGGCT CTCGCAGCCG AAGCCGATCC AACATGGCCC AGGAAGGAGA GACTGAAGCC
    AGTTCTCAAG AGTCTCTGCA AGAGGTCCCC GAGGAAGTGC TGGTCATCTC TCTAGGAACA
    TCACCACAGA CCATTCCCGG GATGATGTCA GAAAACGAGG TTCTGACTTT GCATCTTGGA
    GATGGAGGAC AAGGTGACGC CCCTGTCGAC GACACCAAAT TACATGGCAA GCCTGACAAA
    ACACTGCGCT TCTCACTTTG CAGTGACAAC CTGGAGGGCA TTTCTGAGGG GCCGTCTAAC
    CGCTCAAATT CAGTTTCTTC TTTGGATTTG GAAGTGGAGT CCGTGTCAGA AGGAGGACCA
    GGACCGTCTG GTAGTAACGG TGCAGAAGCC TTACAGCTAC TGGAGCACGA ACAAGCCACT
    ACACAGGATA ATTTGGACGA TAAACTGCGC AAGTTTGAGA TTCGGGACAT GATGGGCCTG
    ACGGATGACC GAGACATTTC TGAGACGGTC AGTGAGACTT GGAGCACTGA TGTCTTGGGG
    AGTGACTTTG ACCCCAACAT GGATGAGGAC CGACTGCAGG AGATAGGGGC TGCAGCGGAG
    AATGCTCTGG GCAGCATGCT GTGTTTGCCT GTGCTTTTGG ATCCATATGG TTCAACCATC
    TCAGAGACCA CGAGCGAGGC TTGGAGTGTG GAGGTTCTGC CGAGTGACTC AGAAGCTGCA
    GACCTGAAGC AGGAGGAACG TTTGCAGGAG CTGGAGAGCT GCTCCGGTGT GGGAAGCACT
    TCAGACGACA CAGAGGTCCG AGAGGTCAGC TCAAGACCAA GTACTCCAGG ACTCAGCGTT
    GTTTCAGGCA TCAGTGCCAC GTCGGAGGAC ATCCCTAATA AGATTGAAGA CCTACGCTCG
    GAATGCAGCT CCGATTTTGG AGGCAAAGAC TCCGTGACCA GTCCAGATGG AGAGGACTCT
    GCTCATGGAG CTCACAGTCT CTCCTGTGCA CCTTCTCAAA CAGATTCACT GCTGGCCATG
    TTCGATCCCC TCTCCTCTGG AGAAGGTTCG TGCACTGGCA CCATAGTGCG ACCCAAGGTA
    CACTATCCTC GTCCTCCTCA CCCTCCGCCT GACCCACCCA TTCCAGAGGC CAGCATAACC
    AGCCACATGG AACCCCGTCC GCCCCTCTTT CCTCCCCACC TGGCACAGGG TGACCTGGAG
    CACCCCAAGC AGAGGCACTC TTACCCTGAG AGGCTGGTGA GGAGCCGCAG CACAGATGTG
    GTGTCCGCTG GACGCAGACC TAACAGTGAC CCCGGACTGA ACCGCAGAAC CATGATGGAG
    GAGAGAGACC CGGCTGGAGC TTACTCTACT GGGCCCACCT CCTCTCCCAG CAAGGATTCC
    CTCAAAGGAG AGTCTGAGGA CAGGAAGGAC AGTGATGATG AGAAGTCTGA TAGGAACAGA
    CCCTGGTGGA AGAAGCGCTT TGTTCCTGCC ATTCCTAAAG TGCTCTATTG GACGGCAGAG
    GGTGAACTCC CAGTGCCGAT TGCCGGGCTT CGGAAGCGGG ACAAGGCAGA AAAAGACGAG
    CAAGGAGCAG AGAGGGTCCC ACAAGATGAG CCTTCACTGC TCAGACAGAG CTCCAAAACC
    CAGTCAGCAG AGGCAATTAT GGACAAGTAC ATCAGGTACA TGGAGAAACG ACCCGCACAG
    GCTGACGGAG CAACTGCTGG AGGTTATGTT GGACAAGAGT CTTGTCGAGA AGGGGACAGT
    GAGCACGACT CTCCTAAAGA TGAAGCTCTG CAGAACATCT CTGCTGATGA TCTTCCGGAT
    TCTGCCAGTC AGACGGCACC ACCTCAGGAC AAGACTTTCT CCTTCAGTGA TATTAAGAAG
    AAGCTACGCC TGGCACTTTG CTCTGCAGAT TCAGTTGTGT TTCCCATCAT TCCACCTGCA
    ACAACACGCA ACGGTCTTCC AGATCATGCA GATCCTGAAG ACAACGAGAT TGTGTGCTTT
    CTGAAGGTGC AGCTGGCGGA GGCCATTAAC CTGCAGGATA AGAACCAGAT GGCACAGATC
    CAGGAGACCA CGCGCTGCGT CAGCCACCTC GACCCGCGCA CCTGCAGAAA ACTGCTCACT
    GCCATGGCAG AGGACTACAG GAAGCGGGCT CCATACATCG CCTATCTGAC ACGTTGTCGG
    CAGGGCCTGC AGACATCTCA GGCTCATCTG GAGAGACTCC TGCAGAGAGT GTTGAGAGAT
    AAAGAAGTGG CCAACCGCTA CTTTACCACT GTCTGTGTCC GACTACTGCT GGAGCACATG
    GAGTCCAAGA TGCTTGACTT CATTAAAGCA TTTCAGGGAT GCACAGCAGC TGATGATAAG
    ACAGCAGCCG TGGAGGATTT CCTGCGTTAC CTGTATGGAG CCATGGCCCA TGATGCCATC
    TGGCAGTACG CCAGTGAAGA GCAGCTCCAG GACGCACAGA TGGCCATCGA ACGCAGCGTC
    ATGAACCGCA TCTTCAAACT GGCATTCTAC CCCAATCAGG ATGGGGACAT CCACAGAGAC
    GAGCTATTCC ATGAGCACAT TCAGCGACTG TCTAGAGTGG TGTCGGCCAA TCATAAAGCT
    CTGCAGATCC CTGAGGTGTA TCTGAAAGAG GCTCCGTGGC CTTCTGCACA GGCTGAGATC
    AAGACCATCA ACGCCTATAA GACCCCGCGA GATAAAGTGC AGTGTATCTT GCGCATGTGC
    TCCACCATTA TGAACCTGCT CAGCCTGGCC AATGAGGATG CTGTACCCGG CGCGGATGAC
    TTCGTCCCTG TGCTCGTCTT TGTTCTGATT AAAGCAAACC CACCCTGCCT TCTGTCCACC
    ATTCAGTACA TCAATAATTT CTACGCCAGC AGGCTGAGCG GGGAGGAGTG CTATTGGTGG
    ATGCAGTTCA CTGCTGCCGT GGAATTCATT AAAACCATCG ACGATCGCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302370.1
    CDS202..4575
    Translation

    Target ORF information:

    RefSeq Version XM_009304095.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304095.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGCAGGCT CTCGCAGCCG AAGCCGATCC AACATGGCCC AGGAAGGAGA GACTGAAGCC
    AGTTCTCAAG AGTCTCTGCA AGAGGTCCCC GAGGAAGTGC TGGTCATCTC TCTAGGAACA
    TCACCACAGA CCATTCCCGG GATGATGTCA GAAAACGAGG TTCTGACTTT GCATCTTGGA
    GATGGAGGAC AAGGTGACGC CCCTGTCGAC GACACCAAAT TACATGGCAA GCCTGACAAA
    ACACTGCGCT TCTCACTTTG CAGTGACAAC CTGGAGGGCA TTTCTGAGGG GCCGTCTAAC
    CGCTCAAATT CAGTTTCTTC TTTGGATTTG GAAGTGGAGT CCGTGTCAGA AGGAGGACCA
    GGACCGTCTG GTAGTAACGG TGCAGAAGCC TTACAGCTAC TGGAGCACGA ACAAGCCACT
    ACACAGGATA ATTTGGACGA TAAACTGCGC AAGTTTGAGA TTCGGGACAT GATGGGCCTG
    ACGGATGACC GAGACATTTC TGAGACGGTC AGTGAGACTT GGAGCACTGA TGTCTTGGGG
    AGTGACTTTG ACCCCAACAT GGATGAGGAC CGACTGCAGG AGATAGGGGC TGCAGCGGAG
    AATGCTCTGG GCAGCATGCT GTGTTTGCCT GTGCTTTTGG ATCCATATGG TTCAACCATC
    TCAGAGACCA CGAGCGAGGC TTGGAGTGTG GAGGTTCTGC CGAGTGACTC AGAAGCTGCA
    GACCTGAAGC AGGAGGAACG TTTGCAGGAG CTGGAGAGCT GCTCCGGTGT GGGAAGCACT
    TCAGACGACA CAGAGGTCCG AGAGGTCAGC TCAAGACCAA GTACTCCAGG ACTCAGCGTT
    GTTTCAGGCA TCAGTGCCAC GTCGGAGGAC ATCCCTAATA AGATTGAAGA CCTACGCTCG
    GAATGCAGCT CCGATTTTGG AGGCAAAGAC TCCGTGACCA GTCCAGATGG AGAGGACTCT
    GCTCATGGAG CTCACAGTCT CTCCTGTGCA CCTTCTCAAA CAGATTCACT GCTGGCCATG
    TTCGATCCCC TCTCCTCTGG AGAAGGTTCG TGCACTGGCA CCATAGTGCG ACCCAAGGTA
    CACTATCCTC GTCCTCCTCA CCCTCCGCCT GACCCACCCA TTCCAGAGGC CAGCATAACC
    AGCCACATGG AACCCCGTCC GCCCCTCTTT CCTCCCCACC TGGCACAGGG TGACCTGGAG
    CACCCCAAGC AGAGGCACTC TTACCCTGAG AGGCTGGTGA GGAGCCGCAG CACAGATGTG
    GTGTCCGCTG GACGCAGACC TAACAGTGAC CCCGGACTGA ACCGCAGAAC CATGATGGAG
    GAGAGAGACC CGGCTGGAGC TTACTCTACT GGGCCCACCT CCTCTCCCAG CAAGGATTCC
    CTCAAAGGAG AGTCTGAGGA CAGGAAGGAC AGTGATGATG AGAAGTCTGA TAGGAACAGA
    CCCTGGTGGA AGAAGCGCTT TGTTCCTGCC ATTCCTAAAG TGCTCTATTG GACGGCAGAG
    GGTGAACTCC CAGTGCCGAT TGCCGGGCTT CGGAAGCGGG ACAAGGCAGA AAAAGACGAG
    CAAGGAGCAG AGAGGGTCCC ACAAGATGAG CCTTCACTGC TCAGACAGAG CTCCAAAACC
    CAGTCAGCAG AGGCAATTAT GGACAAGTAC ATCAGGTACA TGGAGAAACG ACCCGCACAG
    GCTGACGGAG CAACTGCTGG AGGTTATGTT GGACAAGAGT CTTGTCGAGA AGGGGACAGT
    GAGCACGACT CTCCTAAAGA TGAAGCTCTG CAGAACATCT CTGCTGATGA TCTTCCGGAT
    TCTGCCAGTC AGACGGCACC ACCTCAGGAC AAGACTTTCT CCTTCAGTGA TATTAAGAAG
    AAGCTACGCC TGGCACTTTG CTCTGCAGAT TCAGTTGTGT TTCCCATCAT TCCACCTGCA
    ACAACACGCA ACGGTCTTCC AGATCATGCA GATCCTGAAG ACAACGAGAT TGTGTGCTTT
    CTGAAGGTGC AGCTGGCGGA GGCCATTAAC CTGCAGGATA AGAACCAGAT GGCACAGATC
    CAGGAGACCA CGCGCTGCGT CAGCCACCTC GACCCGCGCA CCTGCAGAAA ACTGCTCACT
    GCCATGGCAG AGGACTACAG GAAGCGGGCT CCATACATCG CCTATCTGAC ACGTTGTCGG
    CAGGGCCTGC AGACATCTCA GGCTCATCTG GAGAGACTCC TGCAGAGAGT GTTGAGAGAT
    AAAGAAGTGG CCAACCGCTA CTTTACCACT GTCTGTGTCC GACTACTGCT GGAGCACATG
    GAGTCCAAGA TGCTTGACTT CATTAAAGCA TTTCAGGGAT GCACAGCAGC TGATGATAAG
    ACAGCAGCCG TGGAGGATTT CCTGCGTTAC CTGTATGGAG CCATGGCCCA TGATGCCATC
    TGGCAGTACG CCAGTGAAGA GCAGCTCCAG GACGCACAGA TGGCCATCGA ACGCAGCGTC
    ATGAACCGCA TCTTCAAACT GGCATTCTAC CCCAATCAGG ATGGGGACAT CCACAGAGAC
    GAGCTATTCC ATGAGCACAT TCAGCGACTG TCTAGAGTGG TGTCGGCCAA TCATAAAGCT
    CTGCAGATCC CTGAGGTGTA TCTGAAAGAG GCTCCGTGGC CTTCTGCACA GGCTGAGATC
    AAGACCATCA ACGCCTATAA GACCCCGCGA GATAAAGTGC AGTGTATCTT GCGCATGTGC
    TCCACCATTA TGAACCTGCT CAGCCTGGCC AATGAGGATG CTGTACCCGG CGCGGATGAC
    TTCGTCCCTG TGCTCGTCTT TGTTCTGATT AAAGCAAACC CACCCTGCCT TCTGTCCACC
    ATTCAGTACA TCAATAATTT CTACGCCAGC AGGCTGAGCG GGGAGGAGTG CTATTGGTGG
    ATGCAGTTCA CTGCTGCCGT GGAATTCATT AAAACCATCG ACGATCGCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41045
    Clone ID Related Accession (Same CDS sequence) XM_009304095.3 , XM_009304095.2
    Accession Version XM_009304095.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304095.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGCAGGCT CTCGCAGCCG AAGCCGATCC AACATGGCCC AGGAAGGAGA GACTGAAGCC
    AGTTCTCAAG AGTCTCTGCA AGAGGTCCCC GAGGAAGTGC TGGTCATCTC TCTAGGAACA
    TCACCACAGA CCATTCCCGG GATGATGTCA GAAAACGAGG TTCTGACTTT GCATCTTGGA
    GATGGAGGAC AAGGTGACGC CCCTGTCGAC GACACCAAAT TACATGGCAA GCCTGACAAA
    ACACTGCGCT TCTCACTTTG CAGTGACAAC CTGGAGGGCA TTTCTGAGGG GCCGTCTAAC
    CGCTCAAATT CAGTTTCTTC TTTGGATTTG GAAGTGGAGT CCGTGTCAGA AGGAGGACCA
    GGACCGTCTG GTAGTAACGG TGCAGAAGCC TTACAGCTAC TGGAGCACGA ACAAGCCACT
    ACACAGGATA ATTTGGACGA TAAACTGCGC AAGTTTGAGA TTCGGGACAT GATGGGCCTG
    ACGGATGACC GAGACATTTC TGAGACGGTC AGTGAGACTT GGAGCACTGA TGTCTTGGGG
    AGTGACTTTG ACCCCAACAT GGATGAGGAC CGACTGCAGG AGATAGGGGC TGCAGCGGAG
    AATGCTCTGG GCAGCATGCT GTGTTTGCCT GTGCTTTTGG ATCCATATGG TTCAACCATC
    TCAGAGACCA CGAGCGAGGC TTGGAGTGTG GAGGTTCTGC CGAGTGACTC AGAAGCTGCA
    GACCTGAAGC AGGAGGAACG TTTGCAGGAG CTGGAGAGCT GCTCCGGTGT GGGAAGCACT
    TCAGACGACA CAGAGGTCCG AGAGGTCAGC TCAAGACCAA GTACTCCAGG ACTCAGCGTT
    GTTTCAGGCA TCAGTGCCAC GTCGGAGGAC ATCCCTAATA AGATTGAAGA CCTACGCTCG
    GAATGCAGCT CCGATTTTGG AGGCAAAGAC TCCGTGACCA GTCCAGATGG AGAGGACTCT
    GCTCATGGAG CTCACAGTCT CTCCTGTGCA CCTTCTCAAA CAGATTCACT GCTGGCCATG
    TTCGATCCCC TCTCCTCTGG AGAAGGTTCG TGCACTGGCA CCATAGTGCG ACCCAAGGTA
    CACTATCCTC GTCCTCCTCA CCCTCCGCCT GACCCACCCA TTCCAGAGGC CAGCATAACC
    AGCCACATGG AACCCCGTCC GCCCCTCTTT CCTCCCCACC TGGCACAGGG TGACCTGGAG
    CACCCCAAGC AGAGGCACTC TTACCCTGAG AGGCTGGTGA GGAGCCGCAG CACAGATGTG
    GTGTCCGCTG GACGCAGACC TAACAGTGAC CCCGGACTGA ACCGCAGAAC CATGATGGAG
    GAGAGAGACC CGGCTGGAGC TTACTCTACT GGGCCCACCT CCTCTCCCAG CAAGGATTCC
    CTCAAAGGAG AGTCTGAGGA CAGGAAGGAC AGTGATGATG AGAAGTCTGA TAGGAACAGA
    CCCTGGTGGA AGAAGCGCTT TGTTCCTGCC ATTCCTAAAG TGCTCTATTG GACGGCAGAG
    GGTGAACTCC CAGTGCCGAT TGCCGGGCTT CGGAAGCGGG ACAAGGCAGA AAAAGACGAG
    CAAGGAGCAG AGAGGGTCCC ACAAGATGAG CCTTCACTGC TCAGACAGAG CTCCAAAACC
    CAGTCAGCAG AGGCAATTAT GGACAAGTAC ATCAGGTACA TGGAGAAACG ACCCGCACAG
    GCTGACGGAG CAACTGCTGG AGGTTATGTT GGACAAGAGT CTTGTCGAGA AGGGGACAGT
    GAGCACGACT CTCCTAAAGA TGAAGCTCTG CAGAACATCT CTGCTGATGA TCTTCCGGAT
    TCTGCCAGTC AGACGGCACC ACCTCAGGAC AAGACTTTCT CCTTCAGTGA TATTAAGAAG
    AAGCTACGCC TGGCACTTTG CTCTGCAGAT TCAGTTGTGT TTCCCATCAT TCCACCTGCA
    ACAACACGCA ACGGTCTTCC AGATCATGCA GATCCTGAAG ACAACGAGAT TGTGTGCTTT
    CTGAAGGTGC AGCTGGCGGA GGCCATTAAC CTGCAGGATA AGAACCAGAT GGCACAGATC
    CAGGAGACCA CGCGCTGCGT CAGCCACCTC GACCCGCGCA CCTGCAGAAA ACTGCTCACT
    GCCATGGCAG AGGACTACAG GAAGCGGGCT CCATACATCG CCTATCTGAC ACGTTGTCGG
    CAGGGCCTGC AGACATCTCA GGCTCATCTG GAGAGACTCC TGCAGAGAGT GTTGAGAGAT
    AAAGAAGTGG CCAACCGCTA CTTTACCACT GTCTGTGTCC GACTACTGCT GGAGCACATG
    GAGTCCAAGA TGCTTGACTT CATTAAAGCA TTTCAGGGAT GCACAGCAGC TGATGATAAG
    ACAGCAGCCG TGGAGGATTT CCTGCGTTAC CTGTATGGAG CCATGGCCCA TGATGCCATC
    TGGCAGTACG CCAGTGAAGA GCAGCTCCAG GACGCACAGA TGGCCATCGA ACGCAGCGTC
    ATGAACCGCA TCTTCAAACT GGCATTCTAC CCCAATCAGG ATGGGGACAT CCACAGAGAC
    GAGCTATTCC ATGAGCACAT TCAGCGACTG TCTAGAGTGG TGTCGGCCAA TCATAAAGCT
    CTGCAGATCC CTGAGGTGTA TCTGAAAGAG GCTCCGTGGC CTTCTGCACA GGCTGAGATC
    AAGACCATCA ACGCCTATAA GACCCCGCGA GATAAAGTGC AGTGTATCTT GCGCATGTGC
    TCCACCATTA TGAACCTGCT CAGCCTGGCC AATGAGGATG CTGTACCCGG CGCGGATGAC
    TTCGTCCCTG TGCTCGTCTT TGTTCTGATT AAAGCAAACC CACCCTGCCT TCTGTCCACC
    ATTCAGTACA TCAATAATTT CTACGCCAGC AGGCTGAGCG GGGAGGAGTG CTATTGGTGG
    ATGCAGTTCA CTGCTGCCGT GGAATTCATT AAAACCATCG ACGATCGCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302370.1
    CDS200..4573
    Translation

    Target ORF information:

    RefSeq Version XM_009304095.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304095.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGCAGGCT CTCGCAGCCG AAGCCGATCC AACATGGCCC AGGAAGGAGA GACTGAAGCC
    AGTTCTCAAG AGTCTCTGCA AGAGGTCCCC GAGGAAGTGC TGGTCATCTC TCTAGGAACA
    TCACCACAGA CCATTCCCGG GATGATGTCA GAAAACGAGG TTCTGACTTT GCATCTTGGA
    GATGGAGGAC AAGGTGACGC CCCTGTCGAC GACACCAAAT TACATGGCAA GCCTGACAAA
    ACACTGCGCT TCTCACTTTG CAGTGACAAC CTGGAGGGCA TTTCTGAGGG GCCGTCTAAC
    CGCTCAAATT CAGTTTCTTC TTTGGATTTG GAAGTGGAGT CCGTGTCAGA AGGAGGACCA
    GGACCGTCTG GTAGTAACGG TGCAGAAGCC TTACAGCTAC TGGAGCACGA ACAAGCCACT
    ACACAGGATA ATTTGGACGA TAAACTGCGC AAGTTTGAGA TTCGGGACAT GATGGGCCTG
    ACGGATGACC GAGACATTTC TGAGACGGTC AGTGAGACTT GGAGCACTGA TGTCTTGGGG
    AGTGACTTTG ACCCCAACAT GGATGAGGAC CGACTGCAGG AGATAGGGGC TGCAGCGGAG
    AATGCTCTGG GCAGCATGCT GTGTTTGCCT GTGCTTTTGG ATCCATATGG TTCAACCATC
    TCAGAGACCA CGAGCGAGGC TTGGAGTGTG GAGGTTCTGC CGAGTGACTC AGAAGCTGCA
    GACCTGAAGC AGGAGGAACG TTTGCAGGAG CTGGAGAGCT GCTCCGGTGT GGGAAGCACT
    TCAGACGACA CAGAGGTCCG AGAGGTCAGC TCAAGACCAA GTACTCCAGG ACTCAGCGTT
    GTTTCAGGCA TCAGTGCCAC GTCGGAGGAC ATCCCTAATA AGATTGAAGA CCTACGCTCG
    GAATGCAGCT CCGATTTTGG AGGCAAAGAC TCCGTGACCA GTCCAGATGG AGAGGACTCT
    GCTCATGGAG CTCACAGTCT CTCCTGTGCA CCTTCTCAAA CAGATTCACT GCTGGCCATG
    TTCGATCCCC TCTCCTCTGG AGAAGGTTCG TGCACTGGCA CCATAGTGCG ACCCAAGGTA
    CACTATCCTC GTCCTCCTCA CCCTCCGCCT GACCCACCCA TTCCAGAGGC CAGCATAACC
    AGCCACATGG AACCCCGTCC GCCCCTCTTT CCTCCCCACC TGGCACAGGG TGACCTGGAG
    CACCCCAAGC AGAGGCACTC TTACCCTGAG AGGCTGGTGA GGAGCCGCAG CACAGATGTG
    GTGTCCGCTG GACGCAGACC TAACAGTGAC CCCGGACTGA ACCGCAGAAC CATGATGGAG
    GAGAGAGACC CGGCTGGAGC TTACTCTACT GGGCCCACCT CCTCTCCCAG CAAGGATTCC
    CTCAAAGGAG AGTCTGAGGA CAGGAAGGAC AGTGATGATG AGAAGTCTGA TAGGAACAGA
    CCCTGGTGGA AGAAGCGCTT TGTTCCTGCC ATTCCTAAAG TGCTCTATTG GACGGCAGAG
    GGTGAACTCC CAGTGCCGAT TGCCGGGCTT CGGAAGCGGG ACAAGGCAGA AAAAGACGAG
    CAAGGAGCAG AGAGGGTCCC ACAAGATGAG CCTTCACTGC TCAGACAGAG CTCCAAAACC
    CAGTCAGCAG AGGCAATTAT GGACAAGTAC ATCAGGTACA TGGAGAAACG ACCCGCACAG
    GCTGACGGAG CAACTGCTGG AGGTTATGTT GGACAAGAGT CTTGTCGAGA AGGGGACAGT
    GAGCACGACT CTCCTAAAGA TGAAGCTCTG CAGAACATCT CTGCTGATGA TCTTCCGGAT
    TCTGCCAGTC AGACGGCACC ACCTCAGGAC AAGACTTTCT CCTTCAGTGA TATTAAGAAG
    AAGCTACGCC TGGCACTTTG CTCTGCAGAT TCAGTTGTGT TTCCCATCAT TCCACCTGCA
    ACAACACGCA ACGGTCTTCC AGATCATGCA GATCCTGAAG ACAACGAGAT TGTGTGCTTT
    CTGAAGGTGC AGCTGGCGGA GGCCATTAAC CTGCAGGATA AGAACCAGAT GGCACAGATC
    CAGGAGACCA CGCGCTGCGT CAGCCACCTC GACCCGCGCA CCTGCAGAAA ACTGCTCACT
    GCCATGGCAG AGGACTACAG GAAGCGGGCT CCATACATCG CCTATCTGAC ACGTTGTCGG
    CAGGGCCTGC AGACATCTCA GGCTCATCTG GAGAGACTCC TGCAGAGAGT GTTGAGAGAT
    AAAGAAGTGG CCAACCGCTA CTTTACCACT GTCTGTGTCC GACTACTGCT GGAGCACATG
    GAGTCCAAGA TGCTTGACTT CATTAAAGCA TTTCAGGGAT GCACAGCAGC TGATGATAAG
    ACAGCAGCCG TGGAGGATTT CCTGCGTTAC CTGTATGGAG CCATGGCCCA TGATGCCATC
    TGGCAGTACG CCAGTGAAGA GCAGCTCCAG GACGCACAGA TGGCCATCGA ACGCAGCGTC
    ATGAACCGCA TCTTCAAACT GGCATTCTAC CCCAATCAGG ATGGGGACAT CCACAGAGAC
    GAGCTATTCC ATGAGCACAT TCAGCGACTG TCTAGAGTGG TGTCGGCCAA TCATAAAGCT
    CTGCAGATCC CTGAGGTGTA TCTGAAAGAG GCTCCGTGGC CTTCTGCACA GGCTGAGATC
    AAGACCATCA ACGCCTATAA GACCCCGCGA GATAAAGTGC AGTGTATCTT GCGCATGTGC
    TCCACCATTA TGAACCTGCT CAGCCTGGCC AATGAGGATG CTGTACCCGG CGCGGATGAC
    TTCGTCCCTG TGCTCGTCTT TGTTCTGATT AAAGCAAACC CACCCTGCCT TCTGTCCACC
    ATTCAGTACA TCAATAATTT CTACGCCAGC AGGCTGAGCG GGGAGGAGTG CTATTGGTGG
    ATGCAGTTCA CTGCTGCCGT GGAATTCATT AAAACCATCG ACGATCGCAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41046
    Clone ID Related Accession (Same CDS sequence) XM_009304096.2 , XM_009304096.3
    Accession Version XM_009304096.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGCTCTC GCAGCCGAAG CCGATCCAAC ATGGCCCAGG AAGGAGAGAC TGAAGCCAGT
    TCTCAAGAGT CTCTGCAAGA GGTCCCCGAG GAAGTGCTGG TCATCTCTCT AGGAACATCA
    CCACAGACCA TTCCCGGGAT GATGTCAGAA AACGAGGTTC TGACTTTGCA TCTTGGAGAT
    GGAGGACAAG GTGACGCCCC TGTCGACGAC ACCAAATTAC ATGGCAAGCC TGACAAAACA
    CTGCGCTTCT CACTTTGCAG TGACAACCTG GAGGGCATTT CTGAGGGGCC GTCTAACCGC
    TCAAATTCAG TTTCTTCTTT GGATTTGGAA GTGGAGTCCG TGTCAGAAGG AGGACCAGGA
    CCGTCTGGTA GTAACGGTGC AGAAGCCTTA CAGCTACTGG AGCACGAACA AGCCACTACA
    CAGGATAATT TGGACGATAA ACTGCGCAAG TTTGAGATTC GGGACATGAT GGGCCTGACG
    GATGACCGAG ACATTTCTGA GACGGTCAGT GAGACTTGGA GCACTGATGT CTTGGGGAGT
    GACTTTGACC CCAACATGGA TGAGGACCGA CTGCAGGAGA TAGGGGCTGC AGCGGAGAAT
    GCTCTGGGCA GCATGCTGTG TTTGCCTGTG CTTTTGGATC CATATGGTTC AACCATCTCA
    GAGACCACGA GCGAGGCTTG GAGTGTGGAG GTTCTGCCGA GTGACTCAGA AGCTGCAGAC
    CTGAAGCAGG AGGAACGTTT GCAGGAGCTG GAGAGCTGCT CCGGTGTGGG AAGCACTTCA
    GACGACACAG AGGTCCGAGA GGTCAGCTCA AGACCAAGTA CTCCAGGACT CAGCGTTGTT
    TCAGGCATCA GTGCCACGTC GGAGGACATC CCTAATAAGA TTGAAGACCT ACGCTCGGAA
    TGCAGCTCCG ATTTTGGAGG CAAAGACTCC GTGACCAGTC CAGATGGAGA GGACTCTGCT
    CATGGAGCTC ACAGTCTCTC CTGTGCACCT TCTCAAACAG ATTCACTGCT GGCCATGTTC
    GATCCCCTCT CCTCTGGAGA AGGTTCGTGC ACTGGCACCA TAGTGCGACC CAAGGTACAC
    TATCCTCGTC CTCCTCACCC TCCGCCTGAC CCACCCATTC CAGAGGCCAG CATAACCAGC
    CACATGGAAC CCCGTCCGCC CCTCTTTCCT CCCCACCTGG CACAGGGTGA CCTGGAGCAC
    CCCAAGCAGA GGCACTCTTA CCCTGAGAGG CTGGTGAGGA GCCGCAGCAC AGATGTGGTG
    TCCGCTGGAC GCAGACCTAA CAGTGACCCC GGACTGAACC GCAGAACCAT GATGGAGGAG
    AGAGACCCGG CTGGAGCTTA CTCTACTGGG CCCACCTCCT CTCCCAGCAA GGATTCCCTC
    AAAGGAGAGT CTGAGGACAG GAAGGACAGT GATGATGAGA AGTCTGATAG GAACAGACCC
    TGGTGGAAGA AGCGCTTTGT TCCTGCCATT CCTAAAGTGC TCTATTGGAC GGCAGAGGGT
    GAACTCCCAG TGCCGATTGC CGGGCTTCGG AAGCGGGACA AGGCAGAAAA AGACGAGCAA
    GGAGCAGAGA GGGTCCCACA AGATGAGCCT TCACTGCTCA GACAGAGCTC CAAAACCCAG
    TCAGCAGAGG CAATTATGGA CAAGTACATC AGGTACATGG AGAAACGACC CGCACAGGCT
    GACGGAGCAA CTGCTGGAGG TTATGTTGGA CAAGAGTCTT GTCGAGAAGG GGACAGTGAG
    CACGACTCTC CTAAAGATGA AGCTCTGCAG AACATCTCTG CTGATGATCT TCCGGATTCT
    GCCAGTCAGA CGGCACCACC TCAGGACAAG ACTTTCTCCT TCAGTGATAT TAAGAAGAAG
    CTACGCCTGG CACTTTGCTC TGCAGATTCA GTTGTGTTTC CCATCATTCC ACCTGCAACA
    ACACGCAACG GTCTTCCAGA TCATGCAGAT CCTGAAGACA ACGAGATTGT GTGCTTTCTG
    AAGGTGCAGC TGGCGGAGGC CATTAACCTG CAGGATAAGA ACCAGATGGC ACAGATCCAG
    GAGACCACGC GCTGCGTCAG CCACCTCGAC CCGCGCACCT GCAGAAAACT GCTCACTGCC
    ATGGCAGAGG ACTACAGGAA GCGGGCTCCA TACATCGCCT ATCTGACACG TTGTCGGCAG
    GGCCTGCAGA CATCTCAGGC TCATCTGGAG AGACTCCTGC AGAGAGTGTT GAGAGATAAA
    GAAGTGGCCA ACCGCTACTT TACCACTGTC TGTGTCCGAC TACTGCTGGA GCACATGGAG
    TCCAAGATGC TTGACTTCAT TAAAGCATTT CAGGGATGCA CAGCAGCTGA TGATAAGACA
    GCAGCCGTGG AGGATTTCCT GCGTTACCTG TATGGAGCCA TGGCCCATGA TGCCATCTGG
    CAGTACGCCA GTGAAGAGCA GCTCCAGGAC GCACAGATGG CCATCGAACG CAGCGTCATG
    AACCGCATCT TCAAACTGGC ATTCTACCCC AATCAGGATG GGGACATCCA CAGAGACGAG
    CTATTCCATG AGCACATTCA GCGACTGTCT AGAGTGGTGT CGGCCAATCA TAAAGCTCTG
    CAGATCCCTG AGGTGTATCT GAAAGAGGCT CCGTGGCCTT CTGCACAGGC TGAGATCAAG
    ACCATCAACG CCTATAAGAC CCCGCGAGAT AAAGTGCAGT GTATCTTGCG CATGTGCTCC
    ACCATTATGA ACCTGCTCAG CCTGGCCAAT GAGGATGCTG TACCCGGCGC GGATGACTTC
    GTCCCTGTGC TCGTCTTTGT TCTGATTAAA GCAAACCCAC CCTGCCTTCT GTCCACCATT
    CAGTACATCA ATAATTTCTA CGCCAGCAGG CTGAGCGGGG AGGAGTGCTA TTGGTGGATG
    CAGTTCACTG CTGCCGTGGA ATTCATTAAA ACCATCGACG ATCGCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302371.1
    CDS200..4570
    Translation

    Target ORF information:

    RefSeq Version XM_009304096.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304096.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGCTCTC GCAGCCGAAG CCGATCCAAC ATGGCCCAGG AAGGAGAGAC TGAAGCCAGT
    TCTCAAGAGT CTCTGCAAGA GGTCCCCGAG GAAGTGCTGG TCATCTCTCT AGGAACATCA
    CCACAGACCA TTCCCGGGAT GATGTCAGAA AACGAGGTTC TGACTTTGCA TCTTGGAGAT
    GGAGGACAAG GTGACGCCCC TGTCGACGAC ACCAAATTAC ATGGCAAGCC TGACAAAACA
    CTGCGCTTCT CACTTTGCAG TGACAACCTG GAGGGCATTT CTGAGGGGCC GTCTAACCGC
    TCAAATTCAG TTTCTTCTTT GGATTTGGAA GTGGAGTCCG TGTCAGAAGG AGGACCAGGA
    CCGTCTGGTA GTAACGGTGC AGAAGCCTTA CAGCTACTGG AGCACGAACA AGCCACTACA
    CAGGATAATT TGGACGATAA ACTGCGCAAG TTTGAGATTC GGGACATGAT GGGCCTGACG
    GATGACCGAG ACATTTCTGA GACGGTCAGT GAGACTTGGA GCACTGATGT CTTGGGGAGT
    GACTTTGACC CCAACATGGA TGAGGACCGA CTGCAGGAGA TAGGGGCTGC AGCGGAGAAT
    GCTCTGGGCA GCATGCTGTG TTTGCCTGTG CTTTTGGATC CATATGGTTC AACCATCTCA
    GAGACCACGA GCGAGGCTTG GAGTGTGGAG GTTCTGCCGA GTGACTCAGA AGCTGCAGAC
    CTGAAGCAGG AGGAACGTTT GCAGGAGCTG GAGAGCTGCT CCGGTGTGGG AAGCACTTCA
    GACGACACAG AGGTCCGAGA GGTCAGCTCA AGACCAAGTA CTCCAGGACT CAGCGTTGTT
    TCAGGCATCA GTGCCACGTC GGAGGACATC CCTAATAAGA TTGAAGACCT ACGCTCGGAA
    TGCAGCTCCG ATTTTGGAGG CAAAGACTCC GTGACCAGTC CAGATGGAGA GGACTCTGCT
    CATGGAGCTC ACAGTCTCTC CTGTGCACCT TCTCAAACAG ATTCACTGCT GGCCATGTTC
    GATCCCCTCT CCTCTGGAGA AGGTTCGTGC ACTGGCACCA TAGTGCGACC CAAGGTACAC
    TATCCTCGTC CTCCTCACCC TCCGCCTGAC CCACCCATTC CAGAGGCCAG CATAACCAGC
    CACATGGAAC CCCGTCCGCC CCTCTTTCCT CCCCACCTGG CACAGGGTGA CCTGGAGCAC
    CCCAAGCAGA GGCACTCTTA CCCTGAGAGG CTGGTGAGGA GCCGCAGCAC AGATGTGGTG
    TCCGCTGGAC GCAGACCTAA CAGTGACCCC GGACTGAACC GCAGAACCAT GATGGAGGAG
    AGAGACCCGG CTGGAGCTTA CTCTACTGGG CCCACCTCCT CTCCCAGCAA GGATTCCCTC
    AAAGGAGAGT CTGAGGACAG GAAGGACAGT GATGATGAGA AGTCTGATAG GAACAGACCC
    TGGTGGAAGA AGCGCTTTGT TCCTGCCATT CCTAAAGTGC TCTATTGGAC GGCAGAGGGT
    GAACTCCCAG TGCCGATTGC CGGGCTTCGG AAGCGGGACA AGGCAGAAAA AGACGAGCAA
    GGAGCAGAGA GGGTCCCACA AGATGAGCCT TCACTGCTCA GACAGAGCTC CAAAACCCAG
    TCAGCAGAGG CAATTATGGA CAAGTACATC AGGTACATGG AGAAACGACC CGCACAGGCT
    GACGGAGCAA CTGCTGGAGG TTATGTTGGA CAAGAGTCTT GTCGAGAAGG GGACAGTGAG
    CACGACTCTC CTAAAGATGA AGCTCTGCAG AACATCTCTG CTGATGATCT TCCGGATTCT
    GCCAGTCAGA CGGCACCACC TCAGGACAAG ACTTTCTCCT TCAGTGATAT TAAGAAGAAG
    CTACGCCTGG CACTTTGCTC TGCAGATTCA GTTGTGTTTC CCATCATTCC ACCTGCAACA
    ACACGCAACG GTCTTCCAGA TCATGCAGAT CCTGAAGACA ACGAGATTGT GTGCTTTCTG
    AAGGTGCAGC TGGCGGAGGC CATTAACCTG CAGGATAAGA ACCAGATGGC ACAGATCCAG
    GAGACCACGC GCTGCGTCAG CCACCTCGAC CCGCGCACCT GCAGAAAACT GCTCACTGCC
    ATGGCAGAGG ACTACAGGAA GCGGGCTCCA TACATCGCCT ATCTGACACG TTGTCGGCAG
    GGCCTGCAGA CATCTCAGGC TCATCTGGAG AGACTCCTGC AGAGAGTGTT GAGAGATAAA
    GAAGTGGCCA ACCGCTACTT TACCACTGTC TGTGTCCGAC TACTGCTGGA GCACATGGAG
    TCCAAGATGC TTGACTTCAT TAAAGCATTT CAGGGATGCA CAGCAGCTGA TGATAAGACA
    GCAGCCGTGG AGGATTTCCT GCGTTACCTG TATGGAGCCA TGGCCCATGA TGCCATCTGG
    CAGTACGCCA GTGAAGAGCA GCTCCAGGAC GCACAGATGG CCATCGAACG CAGCGTCATG
    AACCGCATCT TCAAACTGGC ATTCTACCCC AATCAGGATG GGGACATCCA CAGAGACGAG
    CTATTCCATG AGCACATTCA GCGACTGTCT AGAGTGGTGT CGGCCAATCA TAAAGCTCTG
    CAGATCCCTG AGGTGTATCT GAAAGAGGCT CCGTGGCCTT CTGCACAGGC TGAGATCAAG
    ACCATCAACG CCTATAAGAC CCCGCGAGAT AAAGTGCAGT GTATCTTGCG CATGTGCTCC
    ACCATTATGA ACCTGCTCAG CCTGGCCAAT GAGGATGCTG TACCCGGCGC GGATGACTTC
    GTCCCTGTGC TCGTCTTTGT TCTGATTAAA GCAAACCCAC CCTGCCTTCT GTCCACCATT
    CAGTACATCA ATAATTTCTA CGCCAGCAGG CTGAGCGGGG AGGAGTGCTA TTGGTGGATG
    CAGTTCACTG CTGCCGTGGA ATTCATTAAA ACCATCGACG ATCGCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41046
    Clone ID Related Accession (Same CDS sequence) XM_009304096.2 , XM_009304096.3
    Accession Version XM_009304096.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304096.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGCTCTC GCAGCCGAAG CCGATCCAAC ATGGCCCAGG AAGGAGAGAC TGAAGCCAGT
    TCTCAAGAGT CTCTGCAAGA GGTCCCCGAG GAAGTGCTGG TCATCTCTCT AGGAACATCA
    CCACAGACCA TTCCCGGGAT GATGTCAGAA AACGAGGTTC TGACTTTGCA TCTTGGAGAT
    GGAGGACAAG GTGACGCCCC TGTCGACGAC ACCAAATTAC ATGGCAAGCC TGACAAAACA
    CTGCGCTTCT CACTTTGCAG TGACAACCTG GAGGGCATTT CTGAGGGGCC GTCTAACCGC
    TCAAATTCAG TTTCTTCTTT GGATTTGGAA GTGGAGTCCG TGTCAGAAGG AGGACCAGGA
    CCGTCTGGTA GTAACGGTGC AGAAGCCTTA CAGCTACTGG AGCACGAACA AGCCACTACA
    CAGGATAATT TGGACGATAA ACTGCGCAAG TTTGAGATTC GGGACATGAT GGGCCTGACG
    GATGACCGAG ACATTTCTGA GACGGTCAGT GAGACTTGGA GCACTGATGT CTTGGGGAGT
    GACTTTGACC CCAACATGGA TGAGGACCGA CTGCAGGAGA TAGGGGCTGC AGCGGAGAAT
    GCTCTGGGCA GCATGCTGTG TTTGCCTGTG CTTTTGGATC CATATGGTTC AACCATCTCA
    GAGACCACGA GCGAGGCTTG GAGTGTGGAG GTTCTGCCGA GTGACTCAGA AGCTGCAGAC
    CTGAAGCAGG AGGAACGTTT GCAGGAGCTG GAGAGCTGCT CCGGTGTGGG AAGCACTTCA
    GACGACACAG AGGTCCGAGA GGTCAGCTCA AGACCAAGTA CTCCAGGACT CAGCGTTGTT
    TCAGGCATCA GTGCCACGTC GGAGGACATC CCTAATAAGA TTGAAGACCT ACGCTCGGAA
    TGCAGCTCCG ATTTTGGAGG CAAAGACTCC GTGACCAGTC CAGATGGAGA GGACTCTGCT
    CATGGAGCTC ACAGTCTCTC CTGTGCACCT TCTCAAACAG ATTCACTGCT GGCCATGTTC
    GATCCCCTCT CCTCTGGAGA AGGTTCGTGC ACTGGCACCA TAGTGCGACC CAAGGTACAC
    TATCCTCGTC CTCCTCACCC TCCGCCTGAC CCACCCATTC CAGAGGCCAG CATAACCAGC
    CACATGGAAC CCCGTCCGCC CCTCTTTCCT CCCCACCTGG CACAGGGTGA CCTGGAGCAC
    CCCAAGCAGA GGCACTCTTA CCCTGAGAGG CTGGTGAGGA GCCGCAGCAC AGATGTGGTG
    TCCGCTGGAC GCAGACCTAA CAGTGACCCC GGACTGAACC GCAGAACCAT GATGGAGGAG
    AGAGACCCGG CTGGAGCTTA CTCTACTGGG CCCACCTCCT CTCCCAGCAA GGATTCCCTC
    AAAGGAGAGT CTGAGGACAG GAAGGACAGT GATGATGAGA AGTCTGATAG GAACAGACCC
    TGGTGGAAGA AGCGCTTTGT TCCTGCCATT CCTAAAGTGC TCTATTGGAC GGCAGAGGGT
    GAACTCCCAG TGCCGATTGC CGGGCTTCGG AAGCGGGACA AGGCAGAAAA AGACGAGCAA
    GGAGCAGAGA GGGTCCCACA AGATGAGCCT TCACTGCTCA GACAGAGCTC CAAAACCCAG
    TCAGCAGAGG CAATTATGGA CAAGTACATC AGGTACATGG AGAAACGACC CGCACAGGCT
    GACGGAGCAA CTGCTGGAGG TTATGTTGGA CAAGAGTCTT GTCGAGAAGG GGACAGTGAG
    CACGACTCTC CTAAAGATGA AGCTCTGCAG AACATCTCTG CTGATGATCT TCCGGATTCT
    GCCAGTCAGA CGGCACCACC TCAGGACAAG ACTTTCTCCT TCAGTGATAT TAAGAAGAAG
    CTACGCCTGG CACTTTGCTC TGCAGATTCA GTTGTGTTTC CCATCATTCC ACCTGCAACA
    ACACGCAACG GTCTTCCAGA TCATGCAGAT CCTGAAGACA ACGAGATTGT GTGCTTTCTG
    AAGGTGCAGC TGGCGGAGGC CATTAACCTG CAGGATAAGA ACCAGATGGC ACAGATCCAG
    GAGACCACGC GCTGCGTCAG CCACCTCGAC CCGCGCACCT GCAGAAAACT GCTCACTGCC
    ATGGCAGAGG ACTACAGGAA GCGGGCTCCA TACATCGCCT ATCTGACACG TTGTCGGCAG
    GGCCTGCAGA CATCTCAGGC TCATCTGGAG AGACTCCTGC AGAGAGTGTT GAGAGATAAA
    GAAGTGGCCA ACCGCTACTT TACCACTGTC TGTGTCCGAC TACTGCTGGA GCACATGGAG
    TCCAAGATGC TTGACTTCAT TAAAGCATTT CAGGGATGCA CAGCAGCTGA TGATAAGACA
    GCAGCCGTGG AGGATTTCCT GCGTTACCTG TATGGAGCCA TGGCCCATGA TGCCATCTGG
    CAGTACGCCA GTGAAGAGCA GCTCCAGGAC GCACAGATGG CCATCGAACG CAGCGTCATG
    AACCGCATCT TCAAACTGGC ATTCTACCCC AATCAGGATG GGGACATCCA CAGAGACGAG
    CTATTCCATG AGCACATTCA GCGACTGTCT AGAGTGGTGT CGGCCAATCA TAAAGCTCTG
    CAGATCCCTG AGGTGTATCT GAAAGAGGCT CCGTGGCCTT CTGCACAGGC TGAGATCAAG
    ACCATCAACG CCTATAAGAC CCCGCGAGAT AAAGTGCAGT GTATCTTGCG CATGTGCTCC
    ACCATTATGA ACCTGCTCAG CCTGGCCAAT GAGGATGCTG TACCCGGCGC GGATGACTTC
    GTCCCTGTGC TCGTCTTTGT TCTGATTAAA GCAAACCCAC CCTGCCTTCT GTCCACCATT
    CAGTACATCA ATAATTTCTA CGCCAGCAGG CTGAGCGGGG AGGAGTGCTA TTGGTGGATG
    CAGTTCACTG CTGCCGTGGA ATTCATTAAA ACCATCGACG ATCGCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302371.1
    CDS202..4572
    Translation

    Target ORF information:

    RefSeq Version XM_009304096.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304096.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC CAGACATTCA CACTCTGGCC CACCACCTGA AGCAGGAACG TCTCTATGTG 
    GCATCAGAGA AGCAGCTTAT CCAGCGGCTC AACAACGAGG TGCTAAAGAC AGCAGAAAGG
    CTCTACCGCG CAGCCTGGAT CGCCAAACAA CAGAGGATCA ACCTCGACCG CCTCATTCTC
    ACTAGTGCCG AGGCCTCACC TGCAGAATGC TGCCAGCATG CCAAGTTTCT GGAGGACACA
    CAGTTTGTGG ATGGTTATAA AACACTGGGC TTTCAGGAGA GCATCTATGG AGAGTTCCTG
    AGCCGATTGA GGGAGAACCC GCGATTGGTG GCGTCCTGTC TGGTGGCAGG AGAGAGACTT
    AATAATGAGC ACACACAGGG GGTCATACAC ACTGTCTTCA CCACTCTCTA TGGCAACTGC
    ATCATGCAGG AGGATGAAAG CTACCTGCTG CAGGTGCTGC GTTATTTGGT AGAATTTGAG
    CTAAAGGAAA ATGACAACCC TCGTCGGTTG TTACGGCGAG GCACGTGTGC CTTCAGCATC
    CTCTTCAAAC TCTTCTCCGA AGGCCTGTAT TCAGCTAAAC TTTTCCTCAC CGCTACTCTC
    CACGAGCCCA TCATGCAGCT TCTGGTAGAG GACGAGGACC ATCTAGAGAC AGATCCAGCC
    AAACTGATAG AGCGCTTTAC CCCAGCCCAG CAGGAGCGCC TGTTTGGGGA GAAAGGCACA
    GACGAGTACC GCAGAAAGGT TCAAGCAGCA GTGGAGGAAA ATGAGGCCAA GCTGGTGGCT
    CTTGTAAACA CCTTTATCGG CTACTTGAAG CAGAACACCT ACTGCTTCCC ACAGAGCCTG
    CGCTGGATCG TGTCTCAGAT GTATAAGACG CTGGCACGAG TGGAGAGGTT GGAGGTTGGG
    GAGGTGAGGA CGATGTGCAC AGACTTGCTG CTCACCTGCT TCATCTGTCC AGCCATCGTC
    AACCCCGAAC AGTACGGCAT CATCTCAGAT GCACCCATCA ATGAGGTGGC ACGCTTCAAC
    CTCATGCAGG TGGGGCAGTT GTTGCAGCAG TTAGCAATGT CTGATGCAGA TGATGGAGAT
    CCTCGACGTA AAAGCAGTTT ATTAAAGTTT GACAAGATGT GTGTTGCCGC CTTTTTGGAC
    GTGGTCATTG GTGGAAGGGC TGTCGAGACT CCACCGATGT CGTCAATGAA TTTACTGGAG
    GGCCTCACAC GGACTGTTGT TTACATGACT CATAATCAGC TTTTAGCTTT GGTGGACTTT
    GTGCGCAGTG TTACAGCAGG CGATCAGCTC AGAGAGGAAG AGCATCTGGC TCTAGAAACT
    CTTCTTGCTA ATGTTCCTCA GTCCCGCACA ACAAAGAGCA ACAGTCTGGA GCTCACGCCC
    TCAAACACAC CCCAGCTCTC CCCTGCTACT ACACCTGCAA ACAAGAAGAA CAGACTCCCC
    ATTGGCTCTC GCAGCCGAAG CCGATCCAAC ATGGCCCAGG AAGGAGAGAC TGAAGCCAGT
    TCTCAAGAGT CTCTGCAAGA GGTCCCCGAG GAAGTGCTGG TCATCTCTCT AGGAACATCA
    CCACAGACCA TTCCCGGGAT GATGTCAGAA AACGAGGTTC TGACTTTGCA TCTTGGAGAT
    GGAGGACAAG GTGACGCCCC TGTCGACGAC ACCAAATTAC ATGGCAAGCC TGACAAAACA
    CTGCGCTTCT CACTTTGCAG TGACAACCTG GAGGGCATTT CTGAGGGGCC GTCTAACCGC
    TCAAATTCAG TTTCTTCTTT GGATTTGGAA GTGGAGTCCG TGTCAGAAGG AGGACCAGGA
    CCGTCTGGTA GTAACGGTGC AGAAGCCTTA CAGCTACTGG AGCACGAACA AGCCACTACA
    CAGGATAATT TGGACGATAA ACTGCGCAAG TTTGAGATTC GGGACATGAT GGGCCTGACG
    GATGACCGAG ACATTTCTGA GACGGTCAGT GAGACTTGGA GCACTGATGT CTTGGGGAGT
    GACTTTGACC CCAACATGGA TGAGGACCGA CTGCAGGAGA TAGGGGCTGC AGCGGAGAAT
    GCTCTGGGCA GCATGCTGTG TTTGCCTGTG CTTTTGGATC CATATGGTTC AACCATCTCA
    GAGACCACGA GCGAGGCTTG GAGTGTGGAG GTTCTGCCGA GTGACTCAGA AGCTGCAGAC
    CTGAAGCAGG AGGAACGTTT GCAGGAGCTG GAGAGCTGCT CCGGTGTGGG AAGCACTTCA
    GACGACACAG AGGTCCGAGA GGTCAGCTCA AGACCAAGTA CTCCAGGACT CAGCGTTGTT
    TCAGGCATCA GTGCCACGTC GGAGGACATC CCTAATAAGA TTGAAGACCT ACGCTCGGAA
    TGCAGCTCCG ATTTTGGAGG CAAAGACTCC GTGACCAGTC CAGATGGAGA GGACTCTGCT
    CATGGAGCTC ACAGTCTCTC CTGTGCACCT TCTCAAACAG ATTCACTGCT GGCCATGTTC
    GATCCCCTCT CCTCTGGAGA AGGTTCGTGC ACTGGCACCA TAGTGCGACC CAAGGTACAC
    TATCCTCGTC CTCCTCACCC TCCGCCTGAC CCACCCATTC CAGAGGCCAG CATAACCAGC
    CACATGGAAC CCCGTCCGCC CCTCTTTCCT CCCCACCTGG CACAGGGTGA CCTGGAGCAC
    CCCAAGCAGA GGCACTCTTA CCCTGAGAGG CTGGTGAGGA GCCGCAGCAC AGATGTGGTG
    TCCGCTGGAC GCAGACCTAA CAGTGACCCC GGACTGAACC GCAGAACCAT GATGGAGGAG
    AGAGACCCGG CTGGAGCTTA CTCTACTGGG CCCACCTCCT CTCCCAGCAA GGATTCCCTC
    AAAGGAGAGT CTGAGGACAG GAAGGACAGT GATGATGAGA AGTCTGATAG GAACAGACCC
    TGGTGGAAGA AGCGCTTTGT TCCTGCCATT CCTAAAGTGC TCTATTGGAC GGCAGAGGGT
    GAACTCCCAG TGCCGATTGC CGGGCTTCGG AAGCGGGACA AGGCAGAAAA AGACGAGCAA
    GGAGCAGAGA GGGTCCCACA AGATGAGCCT TCACTGCTCA GACAGAGCTC CAAAACCCAG
    TCAGCAGAGG CAATTATGGA CAAGTACATC AGGTACATGG AGAAACGACC CGCACAGGCT
    GACGGAGCAA CTGCTGGAGG TTATGTTGGA CAAGAGTCTT GTCGAGAAGG GGACAGTGAG
    CACGACTCTC CTAAAGATGA AGCTCTGCAG AACATCTCTG CTGATGATCT TCCGGATTCT
    GCCAGTCAGA CGGCACCACC TCAGGACAAG ACTTTCTCCT TCAGTGATAT TAAGAAGAAG
    CTACGCCTGG CACTTTGCTC TGCAGATTCA GTTGTGTTTC CCATCATTCC ACCTGCAACA
    ACACGCAACG GTCTTCCAGA TCATGCAGAT CCTGAAGACA ACGAGATTGT GTGCTTTCTG
    AAGGTGCAGC TGGCGGAGGC CATTAACCTG CAGGATAAGA ACCAGATGGC ACAGATCCAG
    GAGACCACGC GCTGCGTCAG CCACCTCGAC CCGCGCACCT GCAGAAAACT GCTCACTGCC
    ATGGCAGAGG ACTACAGGAA GCGGGCTCCA TACATCGCCT ATCTGACACG TTGTCGGCAG
    GGCCTGCAGA CATCTCAGGC TCATCTGGAG AGACTCCTGC AGAGAGTGTT GAGAGATAAA
    GAAGTGGCCA ACCGCTACTT TACCACTGTC TGTGTCCGAC TACTGCTGGA GCACATGGAG
    TCCAAGATGC TTGACTTCAT TAAAGCATTT CAGGGATGCA CAGCAGCTGA TGATAAGACA
    GCAGCCGTGG AGGATTTCCT GCGTTACCTG TATGGAGCCA TGGCCCATGA TGCCATCTGG
    CAGTACGCCA GTGAAGAGCA GCTCCAGGAC GCACAGATGG CCATCGAACG CAGCGTCATG
    AACCGCATCT TCAAACTGGC ATTCTACCCC AATCAGGATG GGGACATCCA CAGAGACGAG
    CTATTCCATG AGCACATTCA GCGACTGTCT AGAGTGGTGT CGGCCAATCA TAAAGCTCTG
    CAGATCCCTG AGGTGTATCT GAAAGAGGCT CCGTGGCCTT CTGCACAGGC TGAGATCAAG
    ACCATCAACG CCTATAAGAC CCCGCGAGAT AAAGTGCAGT GTATCTTGCG CATGTGCTCC
    ACCATTATGA ACCTGCTCAG CCTGGCCAAT GAGGATGCTG TACCCGGCGC GGATGACTTC
    GTCCCTGTGC TCGTCTTTGT TCTGATTAAA GCAAACCCAC CCTGCCTTCT GTCCACCATT
    CAGTACATCA ATAATTTCTA CGCCAGCAGG CTGAGCGGGG AGGAGTGCTA TTGGTGGATG
    CAGTTCACTG CTGCCGTGGA ATTCATTAAA ACCATCGACG ATCGCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Evolution of complexity in the zebrafish synapse proteome.
    Nature communications814613(2017 Mar)
    Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG


    Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
    Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
    Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,