gapvd1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following gapvd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gapvd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa14181 XM_002943148.5
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa14182 XM_004916739.4
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa14183 XM_004916740.4
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa31122 XM_012969064.3
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa31123 XM_031891621.1
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa31124 XM_031891622.1
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa31125 XM_012969067.3
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa31126 XM_031891624.1
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa31127 XM_012969069.3
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa31128 XM_031891625.1
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa46317 XM_018096655.1
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa46318 XM_018096656.1
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa46319 XM_018096657.1
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa46320 XM_018096658.1
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa64952 XM_031891626.1
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa64953 XM_031891627.1
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa14180 XM_004916738.4
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa31121 XM_031891620.1
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa46314 XM_018096652.1
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa46315 XM_018096653.1
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa46316 XM_018096654.1
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa31129 XM_012969071.2
Latest version!
Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OXa14180 XM_004916738.3 Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa31125 XM_012969067.2 Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa31122 XM_012969064.2 Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa31127 XM_012969069.2 Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa14181 XM_002943148.4 Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa14182 XM_004916739.3 Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa14183 XM_004916740.3 Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa14181
    Clone ID Related Accession (Same CDS sequence) XM_002943148.4 , XM_002943148.5
    Accession Version XM_002943148.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4380bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002943148.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG AAGAGGAAAA TCCGTGTTCA
    ACAGGTCCTT CAAATCGTTC CAACTCAGTT TCCTCTTTGG ATCTGGAAGG GGAGTCTGTA
    TCTGAGCTAG GAGGAGGACC ATCCGGAAGC AATGGGGTGG AGGCTTTGCA GTTATTGGAA
    CATGAACAAG CTACCACACA GGATAATCTA GATGACAAAC TGCGCAAATT TGAAATTCGA
    GACATGATGG GATTGACGGA TGATAGAGAT ATATCAGAGA CAGTCAGTGA GACATGGAGC
    ACGGATGTGC TTGGAAGTGA TTTTGACCCC AACATTGATG AAGATCGACT ACAAGAAATT
    GCAGGTGCAG CAGCTGAGAA CATGCTGGGA AGCCTGCTGT GCCTTCCCGG CTCTGGCTCA
    ATACTGCTTG ATCCTTGTAC TGGATCCACT ATATCTGAGA CTACCAGTGA AGCATGGAGT
    GTTGAGGTGC TCCCTAGTGA TTCTGAAGCA CCAGATCTAA AGCAAGAGGA GCGGCTGCAG
    GAACTGGAGA GCTGTTCAGG TTTGGGAAGC ACATCAGATG ACACTGAAGT TAGAGAGGTC
    AGCTCCCGGC CCAGCACTCC AGGACTCAGT GTGGTGTCAG GTATCAGTGC TACCTCAGAG
    GACATCCCAA ACAAGATAGA GGACCTACGC TCAGAGTGCA GCTCTGACTT TGGAGGAAAG
    GATTCTGTTA CAAGTCCTGA GGCAGAAGAG TCTGTTCATG GACCTCACCA TATAACTGCA
    CCTCCAACCC AGACTGAATC CTTGTTGGCC ATGTTTGACC CACTAGCTCC AGCATCCAAT
    GAAGTGGTTC GACCTAAAGT ACACTATGCG AGACCCTCAC ATCCGCCTCC TGACCCGCCT
    GTACTGGAAG GAGCAAGTGG GGGAAATGAA GCTCGTCTTC CTATGTTCGG TTCTCATGTA
    TTTGTTCCCA TGGAGTCTGA AGCTTACAGG CAGAGACACT CATGTCCTGA AAGACTGGTG
    CGCAGTCGCA GCTCCGACAT CGCTTCTTCC ATACGGAGGC CAATGAGTGA CCCTGGCTGG
    GTGCGGCGTG GGGTGAATGA AGATAGGGAG CTGCCAACAG GAACTGCAAT TAATGCACTA
    ATTAATCCCC CCCAGGCTTC ATCCTCCTCA CCAAGCAAGG ACTCTTCCAG GGGAGAGCCA
    GAAGAAAAAA AAGACAGCGA TGATGAGCGA TCAGATAGGA ATAAGCCTTG GTGGAAGAAG
    CGGTTTGTGT CTGCAATGCC CAAAGCTCCT ATCCCATTTA GGAAGAAGGA AAAACACGAG
    AAGGAGAGGG AAGACTTGGT ACAAGACAGG TTTTCAGTAG CAGATGACTC AACATCTCAG
    CTAGGGCCAC ATTCCCAGGC AGCAGAAGAC ATCCTGGCTA AGTACAGGAA TGCTATTAAG
    AGGACAATCC CCAATGAAGG TTCCTCCGTG CCCTATGAAG GAGCAGATGC TGTTGGAGAT
    GGAGAAAGCC TTCATGATTC TCCTCGTGAT GAAGCTTTGC AGAACATGGC CTCAGATGAC
    CTGCTTGATT CTGCCAGCCA AGTAGCCCAC CCACAGGAGC CTGCATTCTC CTATAGGGAT
    GCTAAGAAAA AATTACGCCT GGCCCTGTGC TCTGCGGATT CAGTAGCGTT CCCTGTATTG
    TCTCATTCGA CACGGAATGG CCTGCCAGAT CACACAGACC CAGAAGACAA TGAGATTGTG
    TGCTTCCTTA AAGTCCAACT TGCAGAGGCC ATAAACCTGC AGGACAAAAA CCTGATGGCT
    CAGATCCAGG AAACTATGCG ATGTGTGAGC CGATTTGACA GCCGCTCCTG CAGCAAAATT
    TTGGCATCCA TTGCAGAGGA TTACCGGAAG AGGGCCTCAT ATATTGCATA TCTAACAAGA
    TGCCGACAGG GACTACAGAC CACCCAGGCT CACCTTGAGC GCCTCCTGCA GAGAGTGCTA
    CGAGATAAAG AGGTCTCCAC GCGCTACTTC ACCACTGTCT GTGTGAGGCT TCTACTTGAG
    AGCAAAGAGA AGGAAATCCA TGAATTTATC CAAGATTTTC AAAAGTTAAC TGCAGCTGAT
    GATAAAACAG CACAAGTTGA AGAGTTCTTG CAATCACTTT ACGGGGCTAT GGCACAAGAT
    GTGATTTGGC AAAATGCCAG TGAGGAGCAG CTGCAAGATG CACAGATTGC TATTGAGCGA
    AGCATTATGA ACCGCATCTT TAAGCTGGCC TTCTATCCCA ATCAAGATGC AGATATTCTG
    CGAGATCAGG TCCTGCATGA ACACATTAAA AGGTTGTCTA AAGTGGTGAC TGCCAATCAC
    AGGGCACTGC AGATACCTGA GGTGTATCTG AGGGAGGCTC CTTGGCCATC TGCCCAAACC
    GAAATCCGTA CCATCAGCGC ATACAAGACA CCCAGGGACA AAGTGCAGTG CATATTGCGC
    ATGTGCTCAA CAATCATGAA CCTTCTAAGC CTGGCCAATG AGTACTCTGT CCCAGGAGCA
    GATGATTTTG TTCCTGTGCT GGTTTTTGTC CTTATTAAGG CTAATCCCCC CTGCCTACTA
    TCAACAGTGC AATATATCAG CAACTTCTAT TCCAATCGGC TCACTGGCGA GGAGTCCTAC
    TGGTGGATGC AGTTCACAGC TGCTGTTGAG TTCATTAAAA CCATTGACGA TCGCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002943194.1
    CDS158..4537
    Translation

    Target ORF information:

    RefSeq Version XM_002943148.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002943148.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG AAGAGGAAAA TCCGTGTTCA
    ACAGGTCCTT CAAATCGTTC CAACTCAGTT TCCTCTTTGG ATCTGGAAGG GGAGTCTGTA
    TCTGAGCTAG GAGGAGGACC ATCCGGAAGC AATGGGGTGG AGGCTTTGCA GTTATTGGAA
    CATGAACAAG CTACCACACA GGATAATCTA GATGACAAAC TGCGCAAATT TGAAATTCGA
    GACATGATGG GATTGACGGA TGATAGAGAT ATATCAGAGA CAGTCAGTGA GACATGGAGC
    ACGGATGTGC TTGGAAGTGA TTTTGACCCC AACATTGATG AAGATCGACT ACAAGAAATT
    GCAGGTGCAG CAGCTGAGAA CATGCTGGGA AGCCTGCTGT GCCTTCCCGG CTCTGGCTCA
    ATACTGCTTG ATCCTTGTAC TGGATCCACT ATATCTGAGA CTACCAGTGA AGCATGGAGT
    GTTGAGGTGC TCCCTAGTGA TTCTGAAGCA CCAGATCTAA AGCAAGAGGA GCGGCTGCAG
    GAACTGGAGA GCTGTTCAGG TTTGGGAAGC ACATCAGATG ACACTGAAGT TAGAGAGGTC
    AGCTCCCGGC CCAGCACTCC AGGACTCAGT GTGGTGTCAG GTATCAGTGC TACCTCAGAG
    GACATCCCAA ACAAGATAGA GGACCTACGC TCAGAGTGCA GCTCTGACTT TGGAGGAAAG
    GATTCTGTTA CAAGTCCTGA GGCAGAAGAG TCTGTTCATG GACCTCACCA TATAACTGCA
    CCTCCAACCC AGACTGAATC CTTGTTGGCC ATGTTTGACC CACTAGCTCC AGCATCCAAT
    GAAGTGGTTC GACCTAAAGT ACACTATGCG AGACCCTCAC ATCCGCCTCC TGACCCGCCT
    GTACTGGAAG GAGCAAGTGG GGGAAATGAA GCTCGTCTTC CTATGTTCGG TTCTCATGTA
    TTTGTTCCCA TGGAGTCTGA AGCTTACAGG CAGAGACACT CATGTCCTGA AAGACTGGTG
    CGCAGTCGCA GCTCCGACAT CGCTTCTTCC ATACGGAGGC CAATGAGTGA CCCTGGCTGG
    GTGCGGCGTG GGGTGAATGA AGATAGGGAG CTGCCAACAG GAACTGCAAT TAATGCACTA
    ATTAATCCCC CCCAGGCTTC ATCCTCCTCA CCAAGCAAGG ACTCTTCCAG GGGAGAGCCA
    GAAGAAAAAA AAGACAGCGA TGATGAGCGA TCAGATAGGA ATAAGCCTTG GTGGAAGAAG
    CGGTTTGTGT CTGCAATGCC CAAAGCTCCT ATCCCATTTA GGAAGAAGGA AAAACACGAG
    AAGGAGAGGG AAGACTTGGT ACAAGACAGG TTTTCAGTAG CAGATGACTC AACATCTCAG
    CTAGGGCCAC ATTCCCAGGC AGCAGAAGAC ATCCTGGCTA AGTACAGGAA TGCTATTAAG
    AGGACAATCC CCAATGAAGG TTCCTCCGTG CCCTATGAAG GAGCAGATGC TGTTGGAGAT
    GGAGAAAGCC TTCATGATTC TCCTCGTGAT GAAGCTTTGC AGAACATGGC CTCAGATGAC
    CTGCTTGATT CTGCCAGCCA AGTAGCCCAC CCACAGGAGC CTGCATTCTC CTATAGGGAT
    GCTAAGAAAA AATTACGCCT GGCCCTGTGC TCTGCGGATT CAGTAGCGTT CCCTGTATTG
    TCTCATTCGA CACGGAATGG CCTGCCAGAT CACACAGACC CAGAAGACAA TGAGATTGTG
    TGCTTCCTTA AAGTCCAACT TGCAGAGGCC ATAAACCTGC AGGACAAAAA CCTGATGGCT
    CAGATCCAGG AAACTATGCG ATGTGTGAGC CGATTTGACA GCCGCTCCTG CAGCAAAATT
    TTGGCATCCA TTGCAGAGGA TTACCGGAAG AGGGCCTCAT ATATTGCATA TCTAACAAGA
    TGCCGACAGG GACTACAGAC CACCCAGGCT CACCTTGAGC GCCTCCTGCA GAGAGTGCTA
    CGAGATAAAG AGGTCTCCAC GCGCTACTTC ACCACTGTCT GTGTGAGGCT TCTACTTGAG
    AGCAAAGAGA AGGAAATCCA TGAATTTATC CAAGATTTTC AAAAGTTAAC TGCAGCTGAT
    GATAAAACAG CACAAGTTGA AGAGTTCTTG CAATCACTTT ACGGGGCTAT GGCACAAGAT
    GTGATTTGGC AAAATGCCAG TGAGGAGCAG CTGCAAGATG CACAGATTGC TATTGAGCGA
    AGCATTATGA ACCGCATCTT TAAGCTGGCC TTCTATCCCA ATCAAGATGC AGATATTCTG
    CGAGATCAGG TCCTGCATGA ACACATTAAA AGGTTGTCTA AAGTGGTGAC TGCCAATCAC
    AGGGCACTGC AGATACCTGA GGTGTATCTG AGGGAGGCTC CTTGGCCATC TGCCCAAACC
    GAAATCCGTA CCATCAGCGC ATACAAGACA CCCAGGGACA AAGTGCAGTG CATATTGCGC
    ATGTGCTCAA CAATCATGAA CCTTCTAAGC CTGGCCAATG AGTACTCTGT CCCAGGAGCA
    GATGATTTTG TTCCTGTGCT GGTTTTTGTC CTTATTAAGG CTAATCCCCC CTGCCTACTA
    TCAACAGTGC AATATATCAG CAACTTCTAT TCCAATCGGC TCACTGGCGA GGAGTCCTAC
    TGGTGGATGC AGTTCACAGC TGCTGTTGAG TTCATTAAAA CCATTGACGA TCGCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14182
    Clone ID Related Accession (Same CDS sequence) XM_004916739.3 , XM_004916739.4
    Accession Version XM_004916739.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916739.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG AAGAGGAAAA TCCGTGTTCA
    ACAGGTCCTT CAAATCGTTC CAACTCAGTT TCCTCTTTGG ATCTGGAAGG GGAGTCTGTA
    TCTGAGCTAG GAGGAGGACC ATCCGGAAGC AATGGGGTGG AGGCTTTGCA GTTATTGGAA
    CATGAACAAG CTACCACACA GGATAATCTA GATGACAAAC TGCGCAAATT TGAAATTCGA
    GACATGATGG GATTGACGGA TGATAGAGAT ATATCAGAGA CAGTCAGTGA GACATGGAGC
    ACGGATGTGC TTGGAAGTGA TTTTGACCCC AACATTGATG AAGATCGACT ACAAGAAATT
    GCAGGTGCAG CAGCTGAGAA CATGCTGGGA AGCCTGCTGT GCCTTCCCGG CTCTGGCTCA
    ATACTGCTTG ATCCTTGTAC TGGATCCACT ATATCTGAGA CTACCAGTGA AGCATGGAGT
    GTTGAGGTGC TCCCTAGTGA TTCTGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTA TCAGTGCTAC CTCAGAGGAC
    ATCCCAAACA AGATAGAGGA CCTACGCTCA GAGTGCAGCT CTGACTTTGG AGGAAAGGAT
    TCTGTTACAA GTCCTGAGGC AGAAGAGTCT GTTCATGGAC CTCACCATAT AACTGCACCT
    CCAACCCAGA CTGAATCCTT GTTGGCCATG TTTGACCCAC TAGCTCCAGC ATCCAATGAA
    GTGGTTCGAC CTAAAGTACA CTATGCGAGA CCCTCACATC CGCCTCCTGA CCCGCCTGTA
    CTGGAAGGAG CAAGTGGGGG AAATGAAGCT CGTCTTCCTA TGTTCGGTTC TCATGTATTT
    GTTCCCATGG AGTCTGAAGC TTACAGGCAG AGACACTCAT GTCCTGAAAG ACTGGTGCGC
    AGTCGCAGCT CCGACATCGC TTCTTCCATA CGGAGGCCAA TGAGTGACCC TGGCTGGGTG
    CGGCGTGGGG TGAATGAAGA TAGGGAGCTG CCAACAGGAA CTGCAATTAA TGCACTAATT
    AATCCCCCCC AGGCTTCATC CTCCTCACCA AGCAAGGACT CTTCCAGGGG AGAGCCAGAA
    GAAAAAAAAG ACAGCGATGA TGAGCGATCA GATAGGAATA AGCCTTGGTG GAAGAAGCGG
    TTTGTGTCTG CAATGCCCAA AGCTCCTATC CCATTTAGGA AGAAGGAAAA ACACGAGAAG
    GAGAGGGAAG ACTTGGTACA AGACAGGTTT TCAGTAGCAG ATGACTCAAC ATCTCAGCTA
    GGGCCACATT CCCAGGCAGC AGAAGACATC CTGGCTAAGT ACAGGAATGC TATTAAGAGG
    ACAATCCCCA ATGAAGGTTC CTCCGTGCCC TATGAAGGAG CAGATGCTGT TGGAGATGGA
    GAAAGCCTTC ATGATTCTCC TCGTGATGAA GCTTTGCAGA ACATGGCCTC AGATGACCTG
    CTTGATTCTG CCAGCCAAGT AGCCCACCCA CAGGAGCCTG CATTCTCCTA TAGGGATGCT
    AAGAAAAAAT TACGCCTGGC CCTGTGCTCT GCGGATTCAG TAGCGTTCCC TGTATTGTCT
    CATTCGACAC GGAATGGCCT GCCAGATCAC ACAGACCCAG AAGACAATGA GATTGTGTGC
    TTCCTTAAAG TCCAACTTGC AGAGGCCATA AACCTGCAGG ACAAAAACCT GATGGCTCAG
    ATCCAGGAAA CTATGCGATG TGTGAGCCGA TTTGACAGCC GCTCCTGCAG CAAAATTTTG
    GCATCCATTG CAGAGGATTA CCGGAAGAGG GCCTCATATA TTGCATATCT AACAAGATGC
    CGACAGGGAC TACAGACCAC CCAGGCTCAC CTTGAGCGCC TCCTGCAGAG AGTGCTACGA
    GATAAAGAGG TCTCCACGCG CTACTTCACC ACTGTCTGTG TGAGGCTTCT ACTTGAGAGC
    AAAGAGAAGG AAATCCATGA ATTTATCCAA GATTTTCAAA AGTTAACTGC AGCTGATGAT
    AAAACAGCAC AAGTTGAAGA GTTCTTGCAA TCACTTTACG GGGCTATGGC ACAAGATGTG
    ATTTGGCAAA ATGCCAGTGA GGAGCAGCTG CAAGATGCAC AGATTGCTAT TGAGCGAAGC
    ATTATGAACC GCATCTTTAA GCTGGCCTTC TATCCCAATC AAGATGCAGA TATTCTGCGA
    GATCAGGTCC TGCATGAACA CATTAAAAGG TTGTCTAAAG TGGTGACTGC CAATCACAGG
    GCACTGCAGA TACCTGAGGT GTATCTGAGG GAGGCTCCTT GGCCATCTGC CCAAACCGAA
    ATCCGTACCA TCAGCGCATA CAAGACACCC AGGGACAAAG TGCAGTGCAT ATTGCGCATG
    TGCTCAACAA TCATGAACCT TCTAAGCCTG GCCAATGAGT ACTCTGTCCC AGGAGCAGAT
    GATTTTGTTC CTGTGCTGGT TTTTGTCCTT ATTAAGGCTA ATCCCCCCTG CCTACTATCA
    ACAGTGCAAT ATATCAGCAA CTTCTATTCC AATCGGCTCA CTGGCGAGGA GTCCTACTGG
    TGGATGCAGT TCACAGCTGC TGTTGAGTTC ATTAAAACCA TTGACGATCG CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916796.1
    CDS159..4535
    Translation

    Target ORF information:

    RefSeq Version XM_004916739.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916739.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG AAGAGGAAAA TCCGTGTTCA
    ACAGGTCCTT CAAATCGTTC CAACTCAGTT TCCTCTTTGG ATCTGGAAGG GGAGTCTGTA
    TCTGAGCTAG GAGGAGGACC ATCCGGAAGC AATGGGGTGG AGGCTTTGCA GTTATTGGAA
    CATGAACAAG CTACCACACA GGATAATCTA GATGACAAAC TGCGCAAATT TGAAATTCGA
    GACATGATGG GATTGACGGA TGATAGAGAT ATATCAGAGA CAGTCAGTGA GACATGGAGC
    ACGGATGTGC TTGGAAGTGA TTTTGACCCC AACATTGATG AAGATCGACT ACAAGAAATT
    GCAGGTGCAG CAGCTGAGAA CATGCTGGGA AGCCTGCTGT GCCTTCCCGG CTCTGGCTCA
    ATACTGCTTG ATCCTTGTAC TGGATCCACT ATATCTGAGA CTACCAGTGA AGCATGGAGT
    GTTGAGGTGC TCCCTAGTGA TTCTGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTA TCAGTGCTAC CTCAGAGGAC
    ATCCCAAACA AGATAGAGGA CCTACGCTCA GAGTGCAGCT CTGACTTTGG AGGAAAGGAT
    TCTGTTACAA GTCCTGAGGC AGAAGAGTCT GTTCATGGAC CTCACCATAT AACTGCACCT
    CCAACCCAGA CTGAATCCTT GTTGGCCATG TTTGACCCAC TAGCTCCAGC ATCCAATGAA
    GTGGTTCGAC CTAAAGTACA CTATGCGAGA CCCTCACATC CGCCTCCTGA CCCGCCTGTA
    CTGGAAGGAG CAAGTGGGGG AAATGAAGCT CGTCTTCCTA TGTTCGGTTC TCATGTATTT
    GTTCCCATGG AGTCTGAAGC TTACAGGCAG AGACACTCAT GTCCTGAAAG ACTGGTGCGC
    AGTCGCAGCT CCGACATCGC TTCTTCCATA CGGAGGCCAA TGAGTGACCC TGGCTGGGTG
    CGGCGTGGGG TGAATGAAGA TAGGGAGCTG CCAACAGGAA CTGCAATTAA TGCACTAATT
    AATCCCCCCC AGGCTTCATC CTCCTCACCA AGCAAGGACT CTTCCAGGGG AGAGCCAGAA
    GAAAAAAAAG ACAGCGATGA TGAGCGATCA GATAGGAATA AGCCTTGGTG GAAGAAGCGG
    TTTGTGTCTG CAATGCCCAA AGCTCCTATC CCATTTAGGA AGAAGGAAAA ACACGAGAAG
    GAGAGGGAAG ACTTGGTACA AGACAGGTTT TCAGTAGCAG ATGACTCAAC ATCTCAGCTA
    GGGCCACATT CCCAGGCAGC AGAAGACATC CTGGCTAAGT ACAGGAATGC TATTAAGAGG
    ACAATCCCCA ATGAAGGTTC CTCCGTGCCC TATGAAGGAG CAGATGCTGT TGGAGATGGA
    GAAAGCCTTC ATGATTCTCC TCGTGATGAA GCTTTGCAGA ACATGGCCTC AGATGACCTG
    CTTGATTCTG CCAGCCAAGT AGCCCACCCA CAGGAGCCTG CATTCTCCTA TAGGGATGCT
    AAGAAAAAAT TACGCCTGGC CCTGTGCTCT GCGGATTCAG TAGCGTTCCC TGTATTGTCT
    CATTCGACAC GGAATGGCCT GCCAGATCAC ACAGACCCAG AAGACAATGA GATTGTGTGC
    TTCCTTAAAG TCCAACTTGC AGAGGCCATA AACCTGCAGG ACAAAAACCT GATGGCTCAG
    ATCCAGGAAA CTATGCGATG TGTGAGCCGA TTTGACAGCC GCTCCTGCAG CAAAATTTTG
    GCATCCATTG CAGAGGATTA CCGGAAGAGG GCCTCATATA TTGCATATCT AACAAGATGC
    CGACAGGGAC TACAGACCAC CCAGGCTCAC CTTGAGCGCC TCCTGCAGAG AGTGCTACGA
    GATAAAGAGG TCTCCACGCG CTACTTCACC ACTGTCTGTG TGAGGCTTCT ACTTGAGAGC
    AAAGAGAAGG AAATCCATGA ATTTATCCAA GATTTTCAAA AGTTAACTGC AGCTGATGAT
    AAAACAGCAC AAGTTGAAGA GTTCTTGCAA TCACTTTACG GGGCTATGGC ACAAGATGTG
    ATTTGGCAAA ATGCCAGTGA GGAGCAGCTG CAAGATGCAC AGATTGCTAT TGAGCGAAGC
    ATTATGAACC GCATCTTTAA GCTGGCCTTC TATCCCAATC AAGATGCAGA TATTCTGCGA
    GATCAGGTCC TGCATGAACA CATTAAAAGG TTGTCTAAAG TGGTGACTGC CAATCACAGG
    GCACTGCAGA TACCTGAGGT GTATCTGAGG GAGGCTCCTT GGCCATCTGC CCAAACCGAA
    ATCCGTACCA TCAGCGCATA CAAGACACCC AGGGACAAAG TGCAGTGCAT ATTGCGCATG
    TGCTCAACAA TCATGAACCT TCTAAGCCTG GCCAATGAGT ACTCTGTCCC AGGAGCAGAT
    GATTTTGTTC CTGTGCTGGT TTTTGTCCTT ATTAAGGCTA ATCCCCCCTG CCTACTATCA
    ACAGTGCAAT ATATCAGCAA CTTCTATTCC AATCGGCTCA CTGGCGAGGA GTCCTACTGG
    TGGATGCAGT TCACAGCTGC TGTTGAGTTC ATTAAAACCA TTGACGATCG CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14183
    Clone ID Related Accession (Same CDS sequence) XM_004916740.3 , XM_004916740.4
    Accession Version XM_004916740.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916740.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG GTCCTTCAAA TCGTTCCAAC
    TCAGTTTCCT CTTTGGATCT GGAAGGGGAG TCTGTATCTG AGCTAGGAGG AGGACCATCC
    GGAAGCAATG GGGTGGAGGC TTTGCAGTTA TTGGAACATG AACAAGCTAC CACACAGGAT
    AATCTAGATG ACAAACTGCG CAAATTTGAA ATTCGAGACA TGATGGGATT GACGGATGAT
    AGAGATATAT CAGAGACAGT CAGTGAGACA TGGAGCACGG ATGTGCTTGG AAGTGATTTT
    GACCCCAACA TTGATGAAGA TCGACTACAA GAAATTGCAG GTGCAGCAGC TGAGAACATG
    CTGGGAAGCC TGCTGTGCCT TCCCGGCTCT GGCTCAATAC TGCTTGATCC TTGTACTGGA
    TCCACTATAT CTGAGACTAC CAGTGAAGCA TGGAGTGTTG AGGTGCTCCC TAGTGATTCT
    GAAGCACCAG ATCTAAAGCA AGAGGAGCGG CTGCAGGAAC TGGAGAGCTG TTCAGGTTTG
    GGAAGCACAT CAGATGACAC TGAAGTTAGA GAGGTCAGCT CCCGGCCCAG CACTCCAGGA
    CTCAGTGTGG TGTCAGGTAT CAGTGCTACC TCAGAGGACA TCCCAAACAA GATAGAGGAC
    CTACGCTCAG AGTGCAGCTC TGACTTTGGA GGAAAGGATT CTGTTACAAG TCCTGAGGCA
    GAAGAGTCTG TTCATGGACC TCACCATATA ACTGCACCTC CAACCCAGAC TGAATCCTTG
    TTGGCCATGT TTGACCCACT AGCTCCAGCA TCCAATGAAG TGGTTCGACC TAAAGTACAC
    TATGCGAGAC CCTCACATCC GCCTCCTGAC CCGCCTGTAC TGGAAGGAGC AAGTGGGGGA
    AATGAAGCTC GTCTTCCTAT GTTCGGTTCT CATGTATTTG TTCCCATGGA GTCTGAAGCT
    TACAGGCAGA GACACTCATG TCCTGAAAGA CTGGTGCGCA GTCGCAGCTC CGACATCGCT
    TCTTCCATAC GGAGGCCAAT GAGTGACCCT GGCTGGGTGC GGCGTGGGGT GAATGAAGAT
    AGGGAGCTGC CAACAGGAAC TGCAATTAAT GCACTAATTA ATCCCCCCCA GGCTTCATCC
    TCCTCACCAA GCAAGGACTC TTCCAGGGGA GAGCCAGAAG AAAAAAAAGA CAGCGATGAT
    GAGCGATCAG ATAGGAATAA GCCTTGGTGG AAGAAGCGGT TTGTGTCTGC AATGCCCAAA
    GCTCCTATCC CATTTAGGAA GAAGGAAAAA CACGAGAAGG AGAGGGAAGA CTTGGTACAA
    GACAGGTTTT CAGTAGCAGA TGACTCAACA TCTCAGCTAG GGCCACATTC CCAGGCAGCA
    GAAGACATCC TGGCTAAGTA CAGGAATGCT ATTAAGAGGA CAATCCCCAA TGAAGGTTCC
    TCCGTGCCCT ATGAAGGAGC AGATGCTGTT GGAGATGGAG AAAGCCTTCA TGATTCTCCT
    CGTGATGAAG CTTTGCAGAA CATGGCCTCA GATGACCTGC TTGATTCTGC CAGCCAAGTA
    GCCCACCCAC AGGAGCCTGC ATTCTCCTAT AGGGATGCTA AGAAAAAATT ACGCCTGGCC
    CTGTGCTCTG CGGATTCAGT AGCGTTCCCT GTATTGTCTC ATTCGACACG GAATGGCCTG
    CCAGATCACA CAGACCCAGA AGACAATGAG ATTGTGTGCT TCCTTAAAGT CCAACTTGCA
    GAGGCCATAA ACCTGCAGGA CAAAAACCTG ATGGCTCAGA TCCAGGAAAC TATGCGATGT
    GTGAGCCGAT TTGACAGCCG CTCCTGCAGC AAAATTTTGG CATCCATTGC AGAGGATTAC
    CGGAAGAGGG CCTCATATAT TGCATATCTA ACAAGATGCC GACAGGGACT ACAGACCACC
    CAGGCTCACC TTGAGCGCCT CCTGCAGAGA GTGCTACGAG ATAAAGAGGT CTCCACGCGC
    TACTTCACCA CTGTCTGTGT GAGGCTTCTA CTTGAGAGCA AAGAGAAGGA AATCCATGAA
    TTTATCCAAG ATTTTCAAAA GTTAACTGCA GCTGATGATA AAACAGCACA AGTTGAAGAG
    TTCTTGCAAT CACTTTACGG GGCTATGGCA CAAGATGTGA TTTGGCAAAA TGCCAGTGAG
    GAGCAGCTGC AAGATGCACA GATTGCTATT GAGCGAAGCA TTATGAACCG CATCTTTAAG
    CTGGCCTTCT ATCCCAATCA AGATGCAGAT ATTCTGCGAG ATCAGGTCCT GCATGAACAC
    ATTAAAAGGT TGTCTAAAGT GGTGACTGCC AATCACAGGG CACTGCAGAT ACCTGAGGTG
    TATCTGAGGG AGGCTCCTTG GCCATCTGCC CAAACCGAAA TCCGTACCAT CAGCGCATAC
    AAGACACCCA GGGACAAAGT GCAGTGCATA TTGCGCATGT GCTCAACAAT CATGAACCTT
    CTAAGCCTGG CCAATGAGTA CTCTGTCCCA GGAGCAGATG ATTTTGTTCC TGTGCTGGTT
    TTTGTCCTTA TTAAGGCTAA TCCCCCCTGC CTACTATCAA CAGTGCAATA TATCAGCAAC
    TTCTATTCCA ATCGGCTCAC TGGCGAGGAG TCCTACTGGT GGATGCAGTT CACAGCTGCT
    GTTGAGTTCA TTAAAACCAT TGACGATCGC AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916797.1
    CDS158..4513
    Translation

    Target ORF information:

    RefSeq Version XM_004916740.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916740.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG GTCCTTCAAA TCGTTCCAAC
    TCAGTTTCCT CTTTGGATCT GGAAGGGGAG TCTGTATCTG AGCTAGGAGG AGGACCATCC
    GGAAGCAATG GGGTGGAGGC TTTGCAGTTA TTGGAACATG AACAAGCTAC CACACAGGAT
    AATCTAGATG ACAAACTGCG CAAATTTGAA ATTCGAGACA TGATGGGATT GACGGATGAT
    AGAGATATAT CAGAGACAGT CAGTGAGACA TGGAGCACGG ATGTGCTTGG AAGTGATTTT
    GACCCCAACA TTGATGAAGA TCGACTACAA GAAATTGCAG GTGCAGCAGC TGAGAACATG
    CTGGGAAGCC TGCTGTGCCT TCCCGGCTCT GGCTCAATAC TGCTTGATCC TTGTACTGGA
    TCCACTATAT CTGAGACTAC CAGTGAAGCA TGGAGTGTTG AGGTGCTCCC TAGTGATTCT
    GAAGCACCAG ATCTAAAGCA AGAGGAGCGG CTGCAGGAAC TGGAGAGCTG TTCAGGTTTG
    GGAAGCACAT CAGATGACAC TGAAGTTAGA GAGGTCAGCT CCCGGCCCAG CACTCCAGGA
    CTCAGTGTGG TGTCAGGTAT CAGTGCTACC TCAGAGGACA TCCCAAACAA GATAGAGGAC
    CTACGCTCAG AGTGCAGCTC TGACTTTGGA GGAAAGGATT CTGTTACAAG TCCTGAGGCA
    GAAGAGTCTG TTCATGGACC TCACCATATA ACTGCACCTC CAACCCAGAC TGAATCCTTG
    TTGGCCATGT TTGACCCACT AGCTCCAGCA TCCAATGAAG TGGTTCGACC TAAAGTACAC
    TATGCGAGAC CCTCACATCC GCCTCCTGAC CCGCCTGTAC TGGAAGGAGC AAGTGGGGGA
    AATGAAGCTC GTCTTCCTAT GTTCGGTTCT CATGTATTTG TTCCCATGGA GTCTGAAGCT
    TACAGGCAGA GACACTCATG TCCTGAAAGA CTGGTGCGCA GTCGCAGCTC CGACATCGCT
    TCTTCCATAC GGAGGCCAAT GAGTGACCCT GGCTGGGTGC GGCGTGGGGT GAATGAAGAT
    AGGGAGCTGC CAACAGGAAC TGCAATTAAT GCACTAATTA ATCCCCCCCA GGCTTCATCC
    TCCTCACCAA GCAAGGACTC TTCCAGGGGA GAGCCAGAAG AAAAAAAAGA CAGCGATGAT
    GAGCGATCAG ATAGGAATAA GCCTTGGTGG AAGAAGCGGT TTGTGTCTGC AATGCCCAAA
    GCTCCTATCC CATTTAGGAA GAAGGAAAAA CACGAGAAGG AGAGGGAAGA CTTGGTACAA
    GACAGGTTTT CAGTAGCAGA TGACTCAACA TCTCAGCTAG GGCCACATTC CCAGGCAGCA
    GAAGACATCC TGGCTAAGTA CAGGAATGCT ATTAAGAGGA CAATCCCCAA TGAAGGTTCC
    TCCGTGCCCT ATGAAGGAGC AGATGCTGTT GGAGATGGAG AAAGCCTTCA TGATTCTCCT
    CGTGATGAAG CTTTGCAGAA CATGGCCTCA GATGACCTGC TTGATTCTGC CAGCCAAGTA
    GCCCACCCAC AGGAGCCTGC ATTCTCCTAT AGGGATGCTA AGAAAAAATT ACGCCTGGCC
    CTGTGCTCTG CGGATTCAGT AGCGTTCCCT GTATTGTCTC ATTCGACACG GAATGGCCTG
    CCAGATCACA CAGACCCAGA AGACAATGAG ATTGTGTGCT TCCTTAAAGT CCAACTTGCA
    GAGGCCATAA ACCTGCAGGA CAAAAACCTG ATGGCTCAGA TCCAGGAAAC TATGCGATGT
    GTGAGCCGAT TTGACAGCCG CTCCTGCAGC AAAATTTTGG CATCCATTGC AGAGGATTAC
    CGGAAGAGGG CCTCATATAT TGCATATCTA ACAAGATGCC GACAGGGACT ACAGACCACC
    CAGGCTCACC TTGAGCGCCT CCTGCAGAGA GTGCTACGAG ATAAAGAGGT CTCCACGCGC
    TACTTCACCA CTGTCTGTGT GAGGCTTCTA CTTGAGAGCA AAGAGAAGGA AATCCATGAA
    TTTATCCAAG ATTTTCAAAA GTTAACTGCA GCTGATGATA AAACAGCACA AGTTGAAGAG
    TTCTTGCAAT CACTTTACGG GGCTATGGCA CAAGATGTGA TTTGGCAAAA TGCCAGTGAG
    GAGCAGCTGC AAGATGCACA GATTGCTATT GAGCGAAGCA TTATGAACCG CATCTTTAAG
    CTGGCCTTCT ATCCCAATCA AGATGCAGAT ATTCTGCGAG ATCAGGTCCT GCATGAACAC
    ATTAAAAGGT TGTCTAAAGT GGTGACTGCC AATCACAGGG CACTGCAGAT ACCTGAGGTG
    TATCTGAGGG AGGCTCCTTG GCCATCTGCC CAAACCGAAA TCCGTACCAT CAGCGCATAC
    AAGACACCCA GGGACAAAGT GCAGTGCATA TTGCGCATGT GCTCAACAAT CATGAACCTT
    CTAAGCCTGG CCAATGAGTA CTCTGTCCCA GGAGCAGATG ATTTTGTTCC TGTGCTGGTT
    TTTGTCCTTA TTAAGGCTAA TCCCCCCTGC CTACTATCAA CAGTGCAATA TATCAGCAAC
    TTCTATTCCA ATCGGCTCAC TGGCGAGGAG TCCTACTGGT GGATGCAGTT CACAGCTGCT
    GTTGAGTTCA TTAAAACCAT TGACGATCGC AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31122
    Clone ID Related Accession (Same CDS sequence) XM_012969064.2 , XM_012969064.3
    Accession Version XM_012969064.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969064.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GAAGAGGAAA ATCCGTGTTC AACAGGTCCT TCAAATCGTT CCAACTCAGT TTCCTCTTTG
    GATCTGGAAG GGGAGTCTGT ATCTGAGCTA GGAGGAGGAC CATCCGGAAG CAATGGGGTG
    GAGGCTTTGC AGTTATTGGA ACATGAACAA GCTACCACAC AGGATAATCT AGATGACAAA
    CTGCGCAAAT TTGAAATTCG AGACATGATG GGATTGACGG ATGATAGAGA TATATCAGAG
    ACAGTCAGTG AGACATGGAG CACGGATGTG CTTGGAAGTG ATTTTGACCC CAACATTGAT
    GAAGATCGAC TACAAGAAAT TGCAGGTGCA GCAGCTGAGA ACATGCTGGG AAGCCTGCTG
    TGCCTTCCCG GCTCTGGCTC AATACTGCTT GATCCTTGTA CTGGATCCAC TATATCTGAG
    ACTACCAGTG AAGCATGGAG TGTTGAGGTG CTCCCTAGTG ATTCTGAAGC ACCAGATCTA
    AAGCAAGAGG AGCGGCTGCA GGAACTGGAG AGCTGTTCAG GTTTGGGAAG CACATCAGAT
    GACACTGAAG TTAGAGAGGT CAGCTCCCGG CCCAGCACTC CAGGACTCAG TGTGGTGTCA
    GGTGACTTTC CCAAACCTTT CTATTCATTG CCGGGTATCA GTGCTACCTC AGAGGACATC
    CCAAACAAGA TAGAGGACCT ACGCTCAGAG TGCAGCTCTG ACTTTGGAGG AAAGGATTCT
    GTTACAAGTC CTGAGGCAGA AGAGTCTGTT CATGGACCTC ACCATATAAC TGCACCTCCA
    ACCCAGACTG AATCCTTGTT GGCCATGTTT GACCCACTAG CTCCAGCATC CAATGAAGTG
    GTTCGACCTA AAGTACACTA TGCGAGACCC TCACATCCGC CTCCTGACCC GCCTGTACTG
    GAAGGAGCAA GTGGGGGAAA TGAAGCTCGT CTTCCTATGT TCGGTTCTCA TGTATTTGTT
    CCCATGGAGT CTGAAGCTTA CAGGCAGAGA CACTCATGTC CTGAAAGACT GGTGCGCAGT
    CGCAGCTCCG ACATCGCTTC TTCCATACGG AGGCCAATGA GTGACCCTGG CTGGGTGCGG
    CGTGGGGTGA ATGAAGATAG GGAGCTGCCA ACAGGAACTG CAATTAATGC ACTAATTAAT
    CCCCCCCAGG CTTCATCCTC CTCACCAAGC AAGGACTCTT CCAGGGGAGA GCCAGAAGAA
    AAAAAAGACA GCGATGATGA GCGATCAGAT AGGAATAAGC CTTGGTGGAA GAAGCGGTTT
    GTGTCTGCAA TGCCCAAAGC TCCTATCCCA TTTAGGAAGA AGGAAAAACA CGAGAAGGAG
    AGGGAAGACT TGGTACAAGA CAGGTTTTCA GTAGCAGATG ACTCAACATC TCAGCTAGGG
    CCACATTCCC AGGCAGCAGA AGACATCCTG GCTAAGTACA GGAATGCTAT TAAGAGGACA
    ATCCCCAATG AAGGTTCCTC CGTGCCCTAT GAAGGAGCAG ATGCTGTTGG AGATGGAGAA
    AGCCTTCATG ATTCTCCTCG TGATGAAGCT TTGCAGAACA TGGCCTCAGA TGACCTGCTT
    GATTCTGCCA GCCAAGTAGC CCACCCACAG GAGCCTGCAT TCTCCTATAG GGATGCTAAG
    AAAAAATTAC GCCTGGCCCT GTGCTCTGCG GATTCAGTAG CGTTCCCTGT ATTGTCTCAT
    TCGACACGGA ATGGCCTGCC AGATCACACA GACCCAGAAG ACAATGAGAT TGTGTGCTTC
    CTTAAAGTCC AACTTGCAGA GGCCATAAAC CTGCAGGACA AAAACCTGAT GGCTCAGATC
    CAGGAAACTA TGCGATGTGT GAGCCGATTT GACAGCCGCT CCTGCAGCAA AATTTTGGCA
    TCCATTGCAG AGGATTACCG GAAGAGGGCC TCATATATTG CATATCTAAC AAGATGCCGA
    CAGGGACTAC AGACCACCCA GGCTCACCTT GAGCGCCTCC TGCAGAGAGT GCTACGAGAT
    AAAGAGGTCT CCACGCGCTA CTTCACCACT GTCTGTGTGA GGCTTCTACT TGAGAGCAAA
    GAGAAGGAAA TCCATGAATT TATCCAAGAT TTTCAAAAGT TAACTGCAGC TGATGATAAA
    ACAGCACAAG TTGAAGAGTT CTTGCAATCA CTTTACGGGG CTATGGCACA AGATGTGATT
    TGGCAAAATG CCAGTGAGGA GCAGCTGCAA GATGCACAGA TTGCTATTGA GCGAAGCATT
    ATGAACCGCA TCTTTAAGCT GGCCTTCTAT CCCAATCAAG ATGCAGATAT TCTGCGAGAT
    CAGGTCCTGC ATGAACACAT TAAAAGGTTG TCTAAAGTGG TGACTGCCAA TCACAGGGCA
    CTGCAGATAC CTGAGGTGTA TCTGAGGGAG GCTCCTTGGC CATCTGCCCA AACCGAAATC
    CGTACCATCA GCGCATACAA GACACCCAGG GACAAAGTGC AGTGCATATT GCGCATGTGC
    TCAACAATCA TGAACCTTCT AAGCCTGGCC AATGAGTACT CTGTCCCAGG AGCAGATGAT
    TTTGTTCCTG TGCTGGTTTT TGTCCTTATT AAGGCTAATC CCCCCTGCCT ACTATCAACA
    GTGCAATATA TCAGCAACTT CTATTCCAAT CGGCTCACTG GCGAGGAGTC CTACTGGTGG
    ATGCAGTTCA CAGCTGCTGT TGAGTTCATT AAAACCATTG ACGATCGCAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824518.1
    CDS160..4653
    Translation

    Target ORF information:

    RefSeq Version XM_012969064.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969064.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GAAGAGGAAA ATCCGTGTTC AACAGGTCCT TCAAATCGTT CCAACTCAGT TTCCTCTTTG
    GATCTGGAAG GGGAGTCTGT ATCTGAGCTA GGAGGAGGAC CATCCGGAAG CAATGGGGTG
    GAGGCTTTGC AGTTATTGGA ACATGAACAA GCTACCACAC AGGATAATCT AGATGACAAA
    CTGCGCAAAT TTGAAATTCG AGACATGATG GGATTGACGG ATGATAGAGA TATATCAGAG
    ACAGTCAGTG AGACATGGAG CACGGATGTG CTTGGAAGTG ATTTTGACCC CAACATTGAT
    GAAGATCGAC TACAAGAAAT TGCAGGTGCA GCAGCTGAGA ACATGCTGGG AAGCCTGCTG
    TGCCTTCCCG GCTCTGGCTC AATACTGCTT GATCCTTGTA CTGGATCCAC TATATCTGAG
    ACTACCAGTG AAGCATGGAG TGTTGAGGTG CTCCCTAGTG ATTCTGAAGC ACCAGATCTA
    AAGCAAGAGG AGCGGCTGCA GGAACTGGAG AGCTGTTCAG GTTTGGGAAG CACATCAGAT
    GACACTGAAG TTAGAGAGGT CAGCTCCCGG CCCAGCACTC CAGGACTCAG TGTGGTGTCA
    GGTGACTTTC CCAAACCTTT CTATTCATTG CCGGGTATCA GTGCTACCTC AGAGGACATC
    CCAAACAAGA TAGAGGACCT ACGCTCAGAG TGCAGCTCTG ACTTTGGAGG AAAGGATTCT
    GTTACAAGTC CTGAGGCAGA AGAGTCTGTT CATGGACCTC ACCATATAAC TGCACCTCCA
    ACCCAGACTG AATCCTTGTT GGCCATGTTT GACCCACTAG CTCCAGCATC CAATGAAGTG
    GTTCGACCTA AAGTACACTA TGCGAGACCC TCACATCCGC CTCCTGACCC GCCTGTACTG
    GAAGGAGCAA GTGGGGGAAA TGAAGCTCGT CTTCCTATGT TCGGTTCTCA TGTATTTGTT
    CCCATGGAGT CTGAAGCTTA CAGGCAGAGA CACTCATGTC CTGAAAGACT GGTGCGCAGT
    CGCAGCTCCG ACATCGCTTC TTCCATACGG AGGCCAATGA GTGACCCTGG CTGGGTGCGG
    CGTGGGGTGA ATGAAGATAG GGAGCTGCCA ACAGGAACTG CAATTAATGC ACTAATTAAT
    CCCCCCCAGG CTTCATCCTC CTCACCAAGC AAGGACTCTT CCAGGGGAGA GCCAGAAGAA
    AAAAAAGACA GCGATGATGA GCGATCAGAT AGGAATAAGC CTTGGTGGAA GAAGCGGTTT
    GTGTCTGCAA TGCCCAAAGC TCCTATCCCA TTTAGGAAGA AGGAAAAACA CGAGAAGGAG
    AGGGAAGACT TGGTACAAGA CAGGTTTTCA GTAGCAGATG ACTCAACATC TCAGCTAGGG
    CCACATTCCC AGGCAGCAGA AGACATCCTG GCTAAGTACA GGAATGCTAT TAAGAGGACA
    ATCCCCAATG AAGGTTCCTC CGTGCCCTAT GAAGGAGCAG ATGCTGTTGG AGATGGAGAA
    AGCCTTCATG ATTCTCCTCG TGATGAAGCT TTGCAGAACA TGGCCTCAGA TGACCTGCTT
    GATTCTGCCA GCCAAGTAGC CCACCCACAG GAGCCTGCAT TCTCCTATAG GGATGCTAAG
    AAAAAATTAC GCCTGGCCCT GTGCTCTGCG GATTCAGTAG CGTTCCCTGT ATTGTCTCAT
    TCGACACGGA ATGGCCTGCC AGATCACACA GACCCAGAAG ACAATGAGAT TGTGTGCTTC
    CTTAAAGTCC AACTTGCAGA GGCCATAAAC CTGCAGGACA AAAACCTGAT GGCTCAGATC
    CAGGAAACTA TGCGATGTGT GAGCCGATTT GACAGCCGCT CCTGCAGCAA AATTTTGGCA
    TCCATTGCAG AGGATTACCG GAAGAGGGCC TCATATATTG CATATCTAAC AAGATGCCGA
    CAGGGACTAC AGACCACCCA GGCTCACCTT GAGCGCCTCC TGCAGAGAGT GCTACGAGAT
    AAAGAGGTCT CCACGCGCTA CTTCACCACT GTCTGTGTGA GGCTTCTACT TGAGAGCAAA
    GAGAAGGAAA TCCATGAATT TATCCAAGAT TTTCAAAAGT TAACTGCAGC TGATGATAAA
    ACAGCACAAG TTGAAGAGTT CTTGCAATCA CTTTACGGGG CTATGGCACA AGATGTGATT
    TGGCAAAATG CCAGTGAGGA GCAGCTGCAA GATGCACAGA TTGCTATTGA GCGAAGCATT
    ATGAACCGCA TCTTTAAGCT GGCCTTCTAT CCCAATCAAG ATGCAGATAT TCTGCGAGAT
    CAGGTCCTGC ATGAACACAT TAAAAGGTTG TCTAAAGTGG TGACTGCCAA TCACAGGGCA
    CTGCAGATAC CTGAGGTGTA TCTGAGGGAG GCTCCTTGGC CATCTGCCCA AACCGAAATC
    CGTACCATCA GCGCATACAA GACACCCAGG GACAAAGTGC AGTGCATATT GCGCATGTGC
    TCAACAATCA TGAACCTTCT AAGCCTGGCC AATGAGTACT CTGTCCCAGG AGCAGATGAT
    TTTGTTCCTG TGCTGGTTTT TGTCCTTATT AAGGCTAATC CCCCCTGCCT ACTATCAACA
    GTGCAATATA TCAGCAACTT CTATTCCAAT CGGCTCACTG GCGAGGAGTC CTACTGGTGG
    ATGCAGTTCA CAGCTGCTGT TGAGTTCATT AAAACCATTG ACGATCGCAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31123
    Clone ID Related Accession (Same CDS sequence) XM_031891621.1
    Accession Version XM_031891621.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGGTC CTTCAAATCG TTCCAACTCA
    GTTTCCTCTT TGGATCTGGA AGGGGAGTCT GTATCTGAGC TAGGAGGAGG ACCATCCGGA
    AGCAATGGGG TGGAGGCTTT GCAGTTATTG GAACATGAAC AAGCTACCAC ACAGGATAAT
    CTAGATGACA AACTGCGCAA ATTTGAAATT CGAGACATGA TGGGATTGAC GGATGATAGA
    GATATATCAG AGACAGTCAG TGAGACATGG AGCACGGATG TGCTTGGAAG TGATTTTGAC
    CCCAACATTG ATGAAGATCG ACTACAAGAA ATTGCAGGTG CAGCAGCTGA GAACATGCTG
    GGAAGCCTGC TGTGCCTTCC CGGCTCTGGC TCAATACTGC TTGATCCTTG TACTGGATCC
    ACTATATCTG AGACTACCAG TGAAGCATGG AGTGTTGAGG TGCTCCCTAG TGATTCTGAA
    GCACCAGATC TAAAGCAAGA GGAGCGGCTG CAGGAACTGG AGAGCTGTTC AGGTTTGGGA
    AGCACATCAG ATGACACTGA AGTTAGAGAG GTCAGCTCCC GGCCCAGCAC TCCAGGACTC
    AGTGTGGTGT CAGGTGTTGC TCTTTCAGGT ATCAGTGCTA CCTCAGAGGA CATCCCAAAC
    AAGATAGAGG ACCTACGCTC AGAGTGCAGC TCTGACTTTG GAGGAAAGGA TTCTGTTACA
    AGTCCTGAGG CAGAAGAGTC TGTTCATGGA CCTCACCATA TAACTGCACC TCCAACCCAG
    ACTGAATCCT TGTTGGCCAT GTTTGACCCA CTAGCTCCAG CATCCAATGA AGTGGTTCGA
    CCTAAAGTAC ACTATGCGAG ACCCTCACAT CCGCCTCCTG ACCCGCCTGT ACTGGAAGGA
    GCAAGTGGGG GAAATGAAGC TCGTCTTCCT ATGTTCGGTT CTCATGTATT TGTTCCCATG
    GAGTCTGAAG CTTACAGGCA GAGACACTCA TGTCCTGAAA GACTGGTGCG CAGTCGCAGC
    TCCGACATCG CTTCTTCCAT ACGGAGGCCA ATGAGTGACC CTGGCTGGGT GCGGCGTGGG
    GTGAATGAAG ATAGGGAGCT GCCAACAGGA ACTGCAATTA ATGCACTAAT TAATCCCCCC
    CAGGCTTCAT CCTCCTCACC AAGCAAGGAC TCTTCCAGGG GAGAGCCAGA AGAAAAAAAA
    GACAGCGATG ATGAGCGATC AGATAGGAAT AAGCCTTGGT GGAAGAAGCG GTTTGTGTCT
    GCAATGCCCA AAGCTCCTAT CCCATTTAGG AAGAAGGAAA AACACGAGAA GGAGAGGGAA
    GACTTGGTAC AAGACAGGTT TTCAGTAGCA GATGACTCAA CATCTCAGCT AGGGCCACAT
    TCCCAGGCAG CAGAAGACAT CCTGGCTAAG TACAGGAATG CTATTAAGAG GACAATCCCC
    AATGAAGGTT CCTCCGTGCC CTATGAAGGA GCAGATGCTG TTGGAGATGG AGAAAGCCTT
    CATGATTCTC CTCGTGATGA AGCTTTGCAG AACATGGCCT CAGATGACCT GCTTGATTCT
    GCCAGCCAAG TAGCCCACCC ACAGGAGCCT GCATTCTCCT ATAGGGATGC TAAGAAAAAA
    TTACGCCTGG CCCTGTGCTC TGCGGATTCA GTAGCGTTCC CTGTATTGTC TCATTCGACA
    CGGAATGGCC TGCCAGATCA CACAGACCCA GAAGACAATG AGATTGTGTG CTTCCTTAAA
    GTCCAACTTG CAGAGGCCAT AAACCTGCAG GACAAAAACC TGATGGCTCA GATCCAGGAA
    ACTATGCGAT GTGTGAGCCG ATTTGACAGC CGCTCCTGCA GCAAAATTTT GGCATCCATT
    GCAGAGGATT ACCGGAAGAG GGCCTCATAT ATTGCATATC TAACAAGATG CCGACAGGGA
    CTACAGACCA CCCAGGCTCA CCTTGAGCGC CTCCTGCAGA GAGTGCTACG AGATAAAGAG
    GTCTCCACGC GCTACTTCAC CACTGTCTGT GTGAGGCTTC TACTTGAGAG CAAAGAGAAG
    GAAATCCATG AATTTATCCA AGATTTTCAA AAGTTAACTG CAGCTGATGA TAAAACAGCA
    CAAGTTGAAG AGTTCTTGCA ATCACTTTAC GGGGCTATGG CACAAGATGT GATTTGGCAA
    AATGCCAGTG AGGAGCAGCT GCAAGATGCA CAGATTGCTA TTGAGCGAAG CATTATGAAC
    CGCATCTTTA AGCTGGCCTT CTATCCCAAT CAAGATGCAG ATATTCTGCG AGATCAGGTC
    CTGCATGAAC ACATTAAAAG GTTGTCTAAA GTGGTGACTG CCAATCACAG GGCACTGCAG
    ATACCTGAGG TGTATCTGAG GGAGGCTCCT TGGCCATCTG CCCAAACCGA AATCCGTACC
    ATCAGCGCAT ACAAGACACC CAGGGACAAA GTGCAGTGCA TATTGCGCAT GTGCTCAACA
    ATCATGAACC TTCTAAGCCT GGCCAATGAG TACTCTGTCC CAGGAGCAGA TGATTTTGTT
    CCTGTGCTGG TTTTTGTCCT TATTAAGGCT AATCCCCCCT GCCTACTATC AACAGTGCAA
    TATATCAGCA ACTTCTATTC CAATCGGCTC ACTGGCGAGG AGTCCTACTG GTGGATGCAG
    TTCACAGCTG CTGTTGAGTT CATTAAAACC ATTGACGATC GCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031747481.1
    CDS161..4648
    Translation

    Target ORF information:

    RefSeq Version XM_031891621.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031891621.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGGTC CTTCAAATCG TTCCAACTCA
    GTTTCCTCTT TGGATCTGGA AGGGGAGTCT GTATCTGAGC TAGGAGGAGG ACCATCCGGA
    AGCAATGGGG TGGAGGCTTT GCAGTTATTG GAACATGAAC AAGCTACCAC ACAGGATAAT
    CTAGATGACA AACTGCGCAA ATTTGAAATT CGAGACATGA TGGGATTGAC GGATGATAGA
    GATATATCAG AGACAGTCAG TGAGACATGG AGCACGGATG TGCTTGGAAG TGATTTTGAC
    CCCAACATTG ATGAAGATCG ACTACAAGAA ATTGCAGGTG CAGCAGCTGA GAACATGCTG
    GGAAGCCTGC TGTGCCTTCC CGGCTCTGGC TCAATACTGC TTGATCCTTG TACTGGATCC
    ACTATATCTG AGACTACCAG TGAAGCATGG AGTGTTGAGG TGCTCCCTAG TGATTCTGAA
    GCACCAGATC TAAAGCAAGA GGAGCGGCTG CAGGAACTGG AGAGCTGTTC AGGTTTGGGA
    AGCACATCAG ATGACACTGA AGTTAGAGAG GTCAGCTCCC GGCCCAGCAC TCCAGGACTC
    AGTGTGGTGT CAGGTGTTGC TCTTTCAGGT ATCAGTGCTA CCTCAGAGGA CATCCCAAAC
    AAGATAGAGG ACCTACGCTC AGAGTGCAGC TCTGACTTTG GAGGAAAGGA TTCTGTTACA
    AGTCCTGAGG CAGAAGAGTC TGTTCATGGA CCTCACCATA TAACTGCACC TCCAACCCAG
    ACTGAATCCT TGTTGGCCAT GTTTGACCCA CTAGCTCCAG CATCCAATGA AGTGGTTCGA
    CCTAAAGTAC ACTATGCGAG ACCCTCACAT CCGCCTCCTG ACCCGCCTGT ACTGGAAGGA
    GCAAGTGGGG GAAATGAAGC TCGTCTTCCT ATGTTCGGTT CTCATGTATT TGTTCCCATG
    GAGTCTGAAG CTTACAGGCA GAGACACTCA TGTCCTGAAA GACTGGTGCG CAGTCGCAGC
    TCCGACATCG CTTCTTCCAT ACGGAGGCCA ATGAGTGACC CTGGCTGGGT GCGGCGTGGG
    GTGAATGAAG ATAGGGAGCT GCCAACAGGA ACTGCAATTA ATGCACTAAT TAATCCCCCC
    CAGGCTTCAT CCTCCTCACC AAGCAAGGAC TCTTCCAGGG GAGAGCCAGA AGAAAAAAAA
    GACAGCGATG ATGAGCGATC AGATAGGAAT AAGCCTTGGT GGAAGAAGCG GTTTGTGTCT
    GCAATGCCCA AAGCTCCTAT CCCATTTAGG AAGAAGGAAA AACACGAGAA GGAGAGGGAA
    GACTTGGTAC AAGACAGGTT TTCAGTAGCA GATGACTCAA CATCTCAGCT AGGGCCACAT
    TCCCAGGCAG CAGAAGACAT CCTGGCTAAG TACAGGAATG CTATTAAGAG GACAATCCCC
    AATGAAGGTT CCTCCGTGCC CTATGAAGGA GCAGATGCTG TTGGAGATGG AGAAAGCCTT
    CATGATTCTC CTCGTGATGA AGCTTTGCAG AACATGGCCT CAGATGACCT GCTTGATTCT
    GCCAGCCAAG TAGCCCACCC ACAGGAGCCT GCATTCTCCT ATAGGGATGC TAAGAAAAAA
    TTACGCCTGG CCCTGTGCTC TGCGGATTCA GTAGCGTTCC CTGTATTGTC TCATTCGACA
    CGGAATGGCC TGCCAGATCA CACAGACCCA GAAGACAATG AGATTGTGTG CTTCCTTAAA
    GTCCAACTTG CAGAGGCCAT AAACCTGCAG GACAAAAACC TGATGGCTCA GATCCAGGAA
    ACTATGCGAT GTGTGAGCCG ATTTGACAGC CGCTCCTGCA GCAAAATTTT GGCATCCATT
    GCAGAGGATT ACCGGAAGAG GGCCTCATAT ATTGCATATC TAACAAGATG CCGACAGGGA
    CTACAGACCA CCCAGGCTCA CCTTGAGCGC CTCCTGCAGA GAGTGCTACG AGATAAAGAG
    GTCTCCACGC GCTACTTCAC CACTGTCTGT GTGAGGCTTC TACTTGAGAG CAAAGAGAAG
    GAAATCCATG AATTTATCCA AGATTTTCAA AAGTTAACTG CAGCTGATGA TAAAACAGCA
    CAAGTTGAAG AGTTCTTGCA ATCACTTTAC GGGGCTATGG CACAAGATGT GATTTGGCAA
    AATGCCAGTG AGGAGCAGCT GCAAGATGCA CAGATTGCTA TTGAGCGAAG CATTATGAAC
    CGCATCTTTA AGCTGGCCTT CTATCCCAAT CAAGATGCAG ATATTCTGCG AGATCAGGTC
    CTGCATGAAC ACATTAAAAG GTTGTCTAAA GTGGTGACTG CCAATCACAG GGCACTGCAG
    ATACCTGAGG TGTATCTGAG GGAGGCTCCT TGGCCATCTG CCCAAACCGA AATCCGTACC
    ATCAGCGCAT ACAAGACACC CAGGGACAAA GTGCAGTGCA TATTGCGCAT GTGCTCAACA
    ATCATGAACC TTCTAAGCCT GGCCAATGAG TACTCTGTCC CAGGAGCAGA TGATTTTGTT
    CCTGTGCTGG TTTTTGTCCT TATTAAGGCT AATCCCCCCT GCCTACTATC AACAGTGCAA
    TATATCAGCA ACTTCTATTC CAATCGGCTC ACTGGCGAGG AGTCCTACTG GTGGATGCAG
    TTCACAGCTG CTGTTGAGTT CATTAAAACC ATTGACGATC GCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31124
    Clone ID Related Accession (Same CDS sequence) XM_031891622.1
    Accession Version XM_031891622.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGGTC CTTCAAATCG TTCCAACTCA
    GTTTCCTCTT TGGATCTGGA AGGGGAGTCT GTATCTGAGC TAGGAGGAGG ACCATCCGGA
    AGCAATGGGG TGGAGGCTTT GCAGTTATTG GAACATGAAC AAGCTACCAC ACAGGATAAT
    CTAGATGACA AACTGCGCAA ATTTGAAATT CGAGACATGA TGGGATTGAC GGATGATAGA
    GATATATCAG AGACAGTCAG TGAGACATGG AGCACGGATG TGCTTGGAAG TGATTTTGAC
    CCCAACATTG ATGAAGATCG ACTACAAGAA ATTGCAGGTG CAGCAGCTGA GAACATGCTG
    GGAAGCCTGC TGTGCCTTCC CGGCTCTGGC TCAATACTGC TTGATCCTTG TACTGGATCC
    ACTATATCTG AGACTACCAG TGAAGCATGG AGTGTTGAGG TGCTCCCTAG TGATTCTGAA
    GCACCAGATC TAAAGCAAGA GGAGCGGCTG CAGGAACTGG AGAGCTGTTC AGGTTTGGGA
    AGCACATCAG ATGACACTGA AGTTAGAGAG GTCAGCTCCC GGCCCAGCAC TCCAGGACTC
    AGTGTGGTGT CAGGTATCAG TGCTACCTCA GAGGACATCC CAAACAAGAT AGAGGACCTA
    CGCTCAGAGT GCAGCTCTGA CTTTGGAGGA AAGGATTCTG TTACAAGTCC TGAGGCAGAA
    GAGTCTGTTC ATGGACCTCA CCATATAACT GCACCTCCAA CCCAGACTGA ATCCTTGTTG
    GCCATGTTTG ACCCACTAGC TCCAGCATCC AATGAAGTGG TTCGACCTAA AGTACACTAT
    GCGAGACCCT CACATCCGCC TCCTGACCCG CCTGTACTGG AAGGAGCAAG TGGGGGAAAT
    GAAGCTCGTC TTCCTATGTT CGGTTCTCAT GTATTTGTTC CCATGGAGTC TGAAGCTTAC
    AGGCAGAGAC ACTCATGTCC TGAAAGACTG GTGCGCAGTC GCAGCTCCGA CATCGCTTCT
    TCCATACGGA GGCCAATGAG TGACCCTGGC TGGGTGCGGC GTGGGGTGAA TGAAGATAGG
    GAGCTGCCAA CAGGAACTGC AATTAATGCA CTAATTAATC CCCCCCAGGC TTCATCCTCC
    TCACCAAGCA AGGACTCTTC CAGGGGAGAG CCAGAAGAAA AAAAAGACAG CGATGATGAG
    CGATCAGATA GGAATAAGCC TTGGTGGAAG AAGCGGTTTG TGTCTGCAAT GCCCAAAGCT
    CCTATCCCAT TTAGGAAGAA GGAAAAACAC GAGAAGGAGA GGGAAGACTT GGTACAAGAC
    AGGTTTTCAG TAGCAGATGA CTCAACATCT CAGCTAGGGC CACATTCCCA GGCAGCAGAA
    GACATCCTGG CTAAGTACAG GAATGCTATT AAGAGGACAA TCCCCAATGA AGGTTCCTCC
    GTGCCCTATG AAGGAGCAGA TGCTGTTGGA GATGGAGAAA GCCTTCATGA TTCTCCTCGT
    GATGAAGCTT TGCAGAACAT GGCCTCAGAT GACCTGCTTG ATTCTGCCAG CCAAGTAGCC
    CACCCACAGG AGCCTGCATT CTCCTATAGG GATGCTAAGA AAAAATTACG CCTGGCCCTG
    TGCTCTGCGG ATTCAGTAGC GTTCCCTGTA TTGTCTCATT CGACACGGAA TGGCCTGCCA
    GATCACACAG ACCCAGAAGA CAATGAGATT GTGTGCTTCC TTAAAGTCCA ACTTGCAGAG
    GCCATAAACC TGCAGGACAA AAACCTGATG GCTCAGATCC AGGAAACTAT GCGATGTGTG
    AGCCGATTTG ACAGCCGCTC CTGCAGCAAA ATTTTGGCAT CCATTGCAGA GGATTACCGG
    AAGAGGGCCT CATATATTGC ATATCTAACA AGATGCCGAC AGGGACTACA GACCACCCAG
    GCTCACCTTG AGCGCCTCCT GCAGAGAGTG CTACGAGATA AAGAGGTCTC CACGCGCTAC
    TTCACCACTG TCTGTGTGAG GCTTCTACTT GAGAGCAAAG AGAAGGAAAT CCATGAATTT
    ATCCAAGATT TTCAAAAGTT AACTGCAGCT GATGATAAAA CAGCACAAGT TGAAGAGTTC
    TTGCAATCAC TTTACGGGGC TATGGCACAA GATGTGATTT GGCAAAATGC CAGTGAGGAG
    CAGCTGCAAG ATGCACAGAT TGCTATTGAG CGAAGCATTA TGAACCGCAT CTTTAAGCTG
    GCCTTCTATC CCAATCAAGA TGCAGATATT CTGCGAGATC AGGTCCTGCA TGAACACATT
    AAAAGGTTGT CTAAAGTGGT GACTGCCAAT CACAGGGCAC TGCAGATACC TGAGGTGTAT
    CTGAGGGAGG CTCCTTGGCC ATCTGCCCAA ACCGAAATCC GTACCATCAG CGCATACAAG
    ACACCCAGGG ACAAAGTGCA GTGCATATTG CGCATGTGCT CAACAATCAT GAACCTTCTA
    AGCCTGGCCA ATGAGTACTC TGTCCCAGGA GCAGATGATT TTGTTCCTGT GCTGGTTTTT
    GTCCTTATTA AGGCTAATCC CCCCTGCCTA CTATCAACAG TGCAATATAT CAGCAACTTC
    TATTCCAATC GGCTCACTGG CGAGGAGTCC TACTGGTGGA TGCAGTTCAC AGCTGCTGTT
    GAGTTCATTA AAACCATTGA CGATCGCAAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031747482.1
    CDS160..4632
    Translation

    Target ORF information:

    RefSeq Version XM_031891622.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031891622.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGGTC CTTCAAATCG TTCCAACTCA
    GTTTCCTCTT TGGATCTGGA AGGGGAGTCT GTATCTGAGC TAGGAGGAGG ACCATCCGGA
    AGCAATGGGG TGGAGGCTTT GCAGTTATTG GAACATGAAC AAGCTACCAC ACAGGATAAT
    CTAGATGACA AACTGCGCAA ATTTGAAATT CGAGACATGA TGGGATTGAC GGATGATAGA
    GATATATCAG AGACAGTCAG TGAGACATGG AGCACGGATG TGCTTGGAAG TGATTTTGAC
    CCCAACATTG ATGAAGATCG ACTACAAGAA ATTGCAGGTG CAGCAGCTGA GAACATGCTG
    GGAAGCCTGC TGTGCCTTCC CGGCTCTGGC TCAATACTGC TTGATCCTTG TACTGGATCC
    ACTATATCTG AGACTACCAG TGAAGCATGG AGTGTTGAGG TGCTCCCTAG TGATTCTGAA
    GCACCAGATC TAAAGCAAGA GGAGCGGCTG CAGGAACTGG AGAGCTGTTC AGGTTTGGGA
    AGCACATCAG ATGACACTGA AGTTAGAGAG GTCAGCTCCC GGCCCAGCAC TCCAGGACTC
    AGTGTGGTGT CAGGTATCAG TGCTACCTCA GAGGACATCC CAAACAAGAT AGAGGACCTA
    CGCTCAGAGT GCAGCTCTGA CTTTGGAGGA AAGGATTCTG TTACAAGTCC TGAGGCAGAA
    GAGTCTGTTC ATGGACCTCA CCATATAACT GCACCTCCAA CCCAGACTGA ATCCTTGTTG
    GCCATGTTTG ACCCACTAGC TCCAGCATCC AATGAAGTGG TTCGACCTAA AGTACACTAT
    GCGAGACCCT CACATCCGCC TCCTGACCCG CCTGTACTGG AAGGAGCAAG TGGGGGAAAT
    GAAGCTCGTC TTCCTATGTT CGGTTCTCAT GTATTTGTTC CCATGGAGTC TGAAGCTTAC
    AGGCAGAGAC ACTCATGTCC TGAAAGACTG GTGCGCAGTC GCAGCTCCGA CATCGCTTCT
    TCCATACGGA GGCCAATGAG TGACCCTGGC TGGGTGCGGC GTGGGGTGAA TGAAGATAGG
    GAGCTGCCAA CAGGAACTGC AATTAATGCA CTAATTAATC CCCCCCAGGC TTCATCCTCC
    TCACCAAGCA AGGACTCTTC CAGGGGAGAG CCAGAAGAAA AAAAAGACAG CGATGATGAG
    CGATCAGATA GGAATAAGCC TTGGTGGAAG AAGCGGTTTG TGTCTGCAAT GCCCAAAGCT
    CCTATCCCAT TTAGGAAGAA GGAAAAACAC GAGAAGGAGA GGGAAGACTT GGTACAAGAC
    AGGTTTTCAG TAGCAGATGA CTCAACATCT CAGCTAGGGC CACATTCCCA GGCAGCAGAA
    GACATCCTGG CTAAGTACAG GAATGCTATT AAGAGGACAA TCCCCAATGA AGGTTCCTCC
    GTGCCCTATG AAGGAGCAGA TGCTGTTGGA GATGGAGAAA GCCTTCATGA TTCTCCTCGT
    GATGAAGCTT TGCAGAACAT GGCCTCAGAT GACCTGCTTG ATTCTGCCAG CCAAGTAGCC
    CACCCACAGG AGCCTGCATT CTCCTATAGG GATGCTAAGA AAAAATTACG CCTGGCCCTG
    TGCTCTGCGG ATTCAGTAGC GTTCCCTGTA TTGTCTCATT CGACACGGAA TGGCCTGCCA
    GATCACACAG ACCCAGAAGA CAATGAGATT GTGTGCTTCC TTAAAGTCCA ACTTGCAGAG
    GCCATAAACC TGCAGGACAA AAACCTGATG GCTCAGATCC AGGAAACTAT GCGATGTGTG
    AGCCGATTTG ACAGCCGCTC CTGCAGCAAA ATTTTGGCAT CCATTGCAGA GGATTACCGG
    AAGAGGGCCT CATATATTGC ATATCTAACA AGATGCCGAC AGGGACTACA GACCACCCAG
    GCTCACCTTG AGCGCCTCCT GCAGAGAGTG CTACGAGATA AAGAGGTCTC CACGCGCTAC
    TTCACCACTG TCTGTGTGAG GCTTCTACTT GAGAGCAAAG AGAAGGAAAT CCATGAATTT
    ATCCAAGATT TTCAAAAGTT AACTGCAGCT GATGATAAAA CAGCACAAGT TGAAGAGTTC
    TTGCAATCAC TTTACGGGGC TATGGCACAA GATGTGATTT GGCAAAATGC CAGTGAGGAG
    CAGCTGCAAG ATGCACAGAT TGCTATTGAG CGAAGCATTA TGAACCGCAT CTTTAAGCTG
    GCCTTCTATC CCAATCAAGA TGCAGATATT CTGCGAGATC AGGTCCTGCA TGAACACATT
    AAAAGGTTGT CTAAAGTGGT GACTGCCAAT CACAGGGCAC TGCAGATACC TGAGGTGTAT
    CTGAGGGAGG CTCCTTGGCC ATCTGCCCAA ACCGAAATCC GTACCATCAG CGCATACAAG
    ACACCCAGGG ACAAAGTGCA GTGCATATTG CGCATGTGCT CAACAATCAT GAACCTTCTA
    AGCCTGGCCA ATGAGTACTC TGTCCCAGGA GCAGATGATT TTGTTCCTGT GCTGGTTTTT
    GTCCTTATTA AGGCTAATCC CCCCTGCCTA CTATCAACAG TGCAATATAT CAGCAACTTC
    TATTCCAATC GGCTCACTGG CGAGGAGTCC TACTGGTGGA TGCAGTTCAC AGCTGCTGTT
    GAGTTCATTA AAACCATTGA CGATCGCAAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31125
    Clone ID Related Accession (Same CDS sequence) XM_012969067.3 , XM_012969067.2
    Accession Version XM_012969067.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969067.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGAAGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTG ACTTTCCCAA ACCTTTCTAT
    TCATTGCCGG GTATCAGTGC TACCTCAGAG GACATCCCAA ACAAGATAGA GGACCTACGC
    TCAGAGTGCA GCTCTGACTT TGGAGGAAAG GATTCTGTTA CAAGTCCTGA GGCAGAAGAG
    TCTGTTCATG GACCTCACCA TATAACTGCA CCTCCAACCC AGACTGAATC CTTGTTGGCC
    ATGTTTGACC CACTAGCTCC AGCATCCAAT GAAGTGGTTC GACCTAAAGT ACACTATGCG
    AGACCCTCAC ATCCGCCTCC TGACCCGCCT GTACTGGAAG GAGCAAGTGG GGGAAATGAA
    GCTCGTCTTC CTATGTTCGG TTCTCATGTA TTTGTTCCCA TGGAGTCTGA AGCTTACAGG
    CAGAGACACT CATGTCCTGA AAGACTGGTG CGCAGTCGCA GCTCCGACAT CGCTTCTTCC
    ATACGGAGGC CAATGAGTGA CCCTGGCTGG GTGCGGCGTG GGGTGAATGA AGATAGGGAG
    CTGCCAACAG GAACTGCAAT TAATGCACTA ATTAATCCCC CCCAGGCTTC ATCCTCCTCA
    CCAAGCAAGG ACTCTTCCAG GGGAGAGCCA GAAGAAAAAA AAGACAGCGA TGATGAGCGA
    TCAGATAGGA ATAAGCCTTG GTGGAAGAAG CGGTTTGTGT CTGCAATGCC CAAAGCTCCT
    ATCCCATTTA GGAAGAAGGA AAAACACGAG AAGGAGAGGG AAGACTTGGT ACAAGACAGG
    TTTTCAGTAG CAGATGACTC AACATCTCAG CTAGGGCCAC ATTCCCAGGC AGCAGAAGAC
    ATCCTGGCTA AGTACAGGAA TGCTATTAAG AGGACAATCC CCAATGAAGG TTCCTCCGTG
    CCCTATGAAG GAGCAGATGC TGTTGGAGAT GGAGAAAGCC TTCATGATTC TCCTCGTGAT
    GAAGCTTTGC AGAACATGGC CTCAGATGAC CTGCTTGATT CTGCCAGCCA AGTAGCCCAC
    CCACAGGAGC CTGCATTCTC CTATAGGGAT GCTAAGAAAA AATTACGCCT GGCCCTGTGC
    TCTGCGGATT CAGTAGCGTT CCCTGTATTG TCTCATTCGA CACGGAATGG CCTGCCAGAT
    CACACAGACC CAGAAGACAA TGAGATTGTG TGCTTCCTTA AAGTCCAACT TGCAGAGGCC
    ATAAACCTGC AGGACAAAAA CCTGATGGCT CAGATCCAGG AAACTATGCG ATGTGTGAGC
    CGATTTGACA GCCGCTCCTG CAGCAAAATT TTGGCATCCA TTGCAGAGGA TTACCGGAAG
    AGGGCCTCAT ATATTGCATA TCTAACAAGA TGCCGACAGG GACTACAGAC CACCCAGGCT
    CACCTTGAGC GCCTCCTGCA GAGAGTGCTA CGAGATAAAG AGGTCTCCAC GCGCTACTTC
    ACCACTGTCT GTGTGAGGCT TCTACTTGAG AGCAAAGAGA AGGAAATCCA TGAATTTATC
    CAAGATTTTC AAAAGTTAAC TGCAGCTGAT GATAAAACAG CACAAGTTGA AGAGTTCTTG
    CAATCACTTT ACGGGGCTAT GGCACAAGAT GTGATTTGGC AAAATGCCAG TGAGGAGCAG
    CTGCAAGATG CACAGATTGC TATTGAGCGA AGCATTATGA ACCGCATCTT TAAGCTGGCC
    TTCTATCCCA ATCAAGATGC AGATATTCTG CGAGATCAGG TCCTGCATGA ACACATTAAA
    AGGTTGTCTA AAGTGGTGAC TGCCAATCAC AGGGCACTGC AGATACCTGA GGTGTATCTG
    AGGGAGGCTC CTTGGCCATC TGCCCAAACC GAAATCCGTA CCATCAGCGC ATACAAGACA
    CCCAGGGACA AAGTGCAGTG CATATTGCGC ATGTGCTCAA CAATCATGAA CCTTCTAAGC
    CTGGCCAATG AGTACTCTGT CCCAGGAGCA GATGATTTTG TTCCTGTGCT GGTTTTTGTC
    CTTATTAAGG CTAATCCCCC CTGCCTACTA TCAACAGTGC AATATATCAG CAACTTCTAT
    TCCAATCGGC TCACTGGCGA GGAGTCCTAC TGGTGGATGC AGTTCACAGC TGCTGTTGAG
    TTCATTAAAA CCATTGACGA TCGCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824521.1
    CDS160..4629
    Translation

    Target ORF information:

    RefSeq Version XM_012969067.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969067.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGAAGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTG ACTTTCCCAA ACCTTTCTAT
    TCATTGCCGG GTATCAGTGC TACCTCAGAG GACATCCCAA ACAAGATAGA GGACCTACGC
    TCAGAGTGCA GCTCTGACTT TGGAGGAAAG GATTCTGTTA CAAGTCCTGA GGCAGAAGAG
    TCTGTTCATG GACCTCACCA TATAACTGCA CCTCCAACCC AGACTGAATC CTTGTTGGCC
    ATGTTTGACC CACTAGCTCC AGCATCCAAT GAAGTGGTTC GACCTAAAGT ACACTATGCG
    AGACCCTCAC ATCCGCCTCC TGACCCGCCT GTACTGGAAG GAGCAAGTGG GGGAAATGAA
    GCTCGTCTTC CTATGTTCGG TTCTCATGTA TTTGTTCCCA TGGAGTCTGA AGCTTACAGG
    CAGAGACACT CATGTCCTGA AAGACTGGTG CGCAGTCGCA GCTCCGACAT CGCTTCTTCC
    ATACGGAGGC CAATGAGTGA CCCTGGCTGG GTGCGGCGTG GGGTGAATGA AGATAGGGAG
    CTGCCAACAG GAACTGCAAT TAATGCACTA ATTAATCCCC CCCAGGCTTC ATCCTCCTCA
    CCAAGCAAGG ACTCTTCCAG GGGAGAGCCA GAAGAAAAAA AAGACAGCGA TGATGAGCGA
    TCAGATAGGA ATAAGCCTTG GTGGAAGAAG CGGTTTGTGT CTGCAATGCC CAAAGCTCCT
    ATCCCATTTA GGAAGAAGGA AAAACACGAG AAGGAGAGGG AAGACTTGGT ACAAGACAGG
    TTTTCAGTAG CAGATGACTC AACATCTCAG CTAGGGCCAC ATTCCCAGGC AGCAGAAGAC
    ATCCTGGCTA AGTACAGGAA TGCTATTAAG AGGACAATCC CCAATGAAGG TTCCTCCGTG
    CCCTATGAAG GAGCAGATGC TGTTGGAGAT GGAGAAAGCC TTCATGATTC TCCTCGTGAT
    GAAGCTTTGC AGAACATGGC CTCAGATGAC CTGCTTGATT CTGCCAGCCA AGTAGCCCAC
    CCACAGGAGC CTGCATTCTC CTATAGGGAT GCTAAGAAAA AATTACGCCT GGCCCTGTGC
    TCTGCGGATT CAGTAGCGTT CCCTGTATTG TCTCATTCGA CACGGAATGG CCTGCCAGAT
    CACACAGACC CAGAAGACAA TGAGATTGTG TGCTTCCTTA AAGTCCAACT TGCAGAGGCC
    ATAAACCTGC AGGACAAAAA CCTGATGGCT CAGATCCAGG AAACTATGCG ATGTGTGAGC
    CGATTTGACA GCCGCTCCTG CAGCAAAATT TTGGCATCCA TTGCAGAGGA TTACCGGAAG
    AGGGCCTCAT ATATTGCATA TCTAACAAGA TGCCGACAGG GACTACAGAC CACCCAGGCT
    CACCTTGAGC GCCTCCTGCA GAGAGTGCTA CGAGATAAAG AGGTCTCCAC GCGCTACTTC
    ACCACTGTCT GTGTGAGGCT TCTACTTGAG AGCAAAGAGA AGGAAATCCA TGAATTTATC
    CAAGATTTTC AAAAGTTAAC TGCAGCTGAT GATAAAACAG CACAAGTTGA AGAGTTCTTG
    CAATCACTTT ACGGGGCTAT GGCACAAGAT GTGATTTGGC AAAATGCCAG TGAGGAGCAG
    CTGCAAGATG CACAGATTGC TATTGAGCGA AGCATTATGA ACCGCATCTT TAAGCTGGCC
    TTCTATCCCA ATCAAGATGC AGATATTCTG CGAGATCAGG TCCTGCATGA ACACATTAAA
    AGGTTGTCTA AAGTGGTGAC TGCCAATCAC AGGGCACTGC AGATACCTGA GGTGTATCTG
    AGGGAGGCTC CTTGGCCATC TGCCCAAACC GAAATCCGTA CCATCAGCGC ATACAAGACA
    CCCAGGGACA AAGTGCAGTG CATATTGCGC ATGTGCTCAA CAATCATGAA CCTTCTAAGC
    CTGGCCAATG AGTACTCTGT CCCAGGAGCA GATGATTTTG TTCCTGTGCT GGTTTTTGTC
    CTTATTAAGG CTAATCCCCC CTGCCTACTA TCAACAGTGC AATATATCAG CAACTTCTAT
    TCCAATCGGC TCACTGGCGA GGAGTCCTAC TGGTGGATGC AGTTCACAGC TGCTGTTGAG
    TTCATTAAAA CCATTGACGA TCGCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31126
    Clone ID Related Accession (Same CDS sequence) XM_031891624.1
    Accession Version XM_031891624.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGGTC CTTCAAATCG TTCCAACTCA
    GTTTCCTCTT TGGATCTGGA AGGGGAGTCT GTATCTGAGC TAGGAGGAGG ACCATCCGGA
    AGCAATGGGG TGGAGGCTTT GCAGTTATTG GAACATGAAC AAGCTACCAC ACAGGATAAT
    CTAGATGACA AACTGCGCAA ATTTGAAATT CGAGACATGA TGGGATTGAC GGATGATAGA
    GATATATCAG AGACAGTCAG TGAGACATGG AGCACGGATG TGCTTGGAAG TGATTTTGAC
    CCCAACATTG ATGAAGATCG ACTACAAGAA ATTGCAGGTG CAGCAGCTGA GAACATGCTG
    GGAAGCCTGC TGTGCCTTCC CGGCTCTGGC TCAATACTGC TTGATCCTTG TACTGGATCC
    ACTATATCTG AGACTACCAG TGAAGCATGG AGTGTTGAGG TGCTCCCTAG TGATTCTGCA
    CCAGATCTAA AGCAAGAGGA GCGGCTGCAG GAACTGGAGA GCTGTTCAGG TTTGGGAAGC
    ACATCAGATG ACACTGAAGT TAGAGAGGTC AGCTCCCGGC CCAGCACTCC AGGACTCAGT
    GTGGTGTCAG GTATCAGTGC TACCTCAGAG GACATCCCAA ACAAGATAGA GGACCTACGC
    TCAGAGTGCA GCTCTGACTT TGGAGGAAAG GATTCTGTTA CAAGTCCTGA GGCAGAAGAG
    TCTGTTCATG GACCTCACCA TATAACTGCA CCTCCAACCC AGACTGAATC CTTGTTGGCC
    ATGTTTGACC CACTAGCTCC AGCATCCAAT GAAGTGGTTC GACCTAAAGT ACACTATGCG
    AGACCCTCAC ATCCGCCTCC TGACCCGCCT GTACTGGAAG GAGCAAGTGG GGGAAATGAA
    GCTCGTCTTC CTATGTTCGG TTCTCATGTA TTTGTTCCCA TGGAGTCTGA AGCTTACAGG
    CAGAGACACT CATGTCCTGA AAGACTGGTG CGCAGTCGCA GCTCCGACAT CGCTTCTTCC
    ATACGGAGGC CAATGAGTGA CCCTGGCTGG GTGCGGCGTG GGGTGAATGA AGATAGGGAG
    CTGCCAACAG GAACTGCAAT TAATGCACTA ATTAATCCCC CCCAGGCTTC ATCCTCCTCA
    CCAAGCAAGG ACTCTTCCAG GGGAGAGCCA GAAGAAAAAA AAGACAGCGA TGATGAGCGA
    TCAGATAGGA ATAAGCCTTG GTGGAAGAAG CGGTTTGTGT CTGCAATGCC CAAAGCTCCT
    ATCCCATTTA GGAAGAAGGA AAAACACGAG AAGGAGAGGG AAGACTTGGT ACAAGACAGG
    TTTTCAGTAG CAGATGACTC AACATCTCAG CTAGGGCCAC ATTCCCAGGC AGCAGAAGAC
    ATCCTGGCTA AGTACAGGAA TGCTATTAAG AGGACAATCC CCAATGAAGG TTCCTCCGTG
    CCCTATGAAG GAGCAGATGC TGTTGGAGAT GGAGAAAGCC TTCATGATTC TCCTCGTGAT
    GAAGCTTTGC AGAACATGGC CTCAGATGAC CTGCTTGATT CTGCCAGCCA AGTAGCCCAC
    CCACAGGAGC CTGCATTCTC CTATAGGGAT GCTAAGAAAA AATTACGCCT GGCCCTGTGC
    TCTGCGGATT CAGTAGCGTT CCCTGTATTG TCTCATTCGA CACGGAATGG CCTGCCAGAT
    CACACAGACC CAGAAGACAA TGAGATTGTG TGCTTCCTTA AAGTCCAACT TGCAGAGGCC
    ATAAACCTGC AGGACAAAAA CCTGATGGCT CAGATCCAGG AAACTATGCG ATGTGTGAGC
    CGATTTGACA GCCGCTCCTG CAGCAAAATT TTGGCATCCA TTGCAGAGGA TTACCGGAAG
    AGGGCCTCAT ATATTGCATA TCTAACAAGA TGCCGACAGG GACTACAGAC CACCCAGGCT
    CACCTTGAGC GCCTCCTGCA GAGAGTGCTA CGAGATAAAG AGGTCTCCAC GCGCTACTTC
    ACCACTGTCT GTGTGAGGCT TCTACTTGAG AGCAAAGAGA AGGAAATCCA TGAATTTATC
    CAAGATTTTC AAAAGTTAAC TGCAGCTGAT GATAAAACAG CACAAGTTGA AGAGTTCTTG
    CAATCACTTT ACGGGGCTAT GGCACAAGAT GTGATTTGGC AAAATGCCAG TGAGGAGCAG
    CTGCAAGATG CACAGATTGC TATTGAGCGA AGCATTATGA ACCGCATCTT TAAGCTGGCC
    TTCTATCCCA ATCAAGATGC AGATATTCTG CGAGATCAGG TCCTGCATGA ACACATTAAA
    AGGTTGTCTA AAGTGGTGAC TGCCAATCAC AGGGCACTGC AGATACCTGA GGTGTATCTG
    AGGGAGGCTC CTTGGCCATC TGCCCAAACC GAAATCCGTA CCATCAGCGC ATACAAGACA
    CCCAGGGACA AAGTGCAGTG CATATTGCGC ATGTGCTCAA CAATCATGAA CCTTCTAAGC
    CTGGCCAATG AGTACTCTGT CCCAGGAGCA GATGATTTTG TTCCTGTGCT GGTTTTTGTC
    CTTATTAAGG CTAATCCCCC CTGCCTACTA TCAACAGTGC AATATATCAG CAACTTCTAT
    TCCAATCGGC TCACTGGCGA GGAGTCCTAC TGGTGGATGC AGTTCACAGC TGCTGTTGAG
    TTCATTAAAA CCATTGACGA TCGCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031747484.1
    CDS161..4630
    Translation

    Target ORF information:

    RefSeq Version XM_031891624.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031891624.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGGTC CTTCAAATCG TTCCAACTCA
    GTTTCCTCTT TGGATCTGGA AGGGGAGTCT GTATCTGAGC TAGGAGGAGG ACCATCCGGA
    AGCAATGGGG TGGAGGCTTT GCAGTTATTG GAACATGAAC AAGCTACCAC ACAGGATAAT
    CTAGATGACA AACTGCGCAA ATTTGAAATT CGAGACATGA TGGGATTGAC GGATGATAGA
    GATATATCAG AGACAGTCAG TGAGACATGG AGCACGGATG TGCTTGGAAG TGATTTTGAC
    CCCAACATTG ATGAAGATCG ACTACAAGAA ATTGCAGGTG CAGCAGCTGA GAACATGCTG
    GGAAGCCTGC TGTGCCTTCC CGGCTCTGGC TCAATACTGC TTGATCCTTG TACTGGATCC
    ACTATATCTG AGACTACCAG TGAAGCATGG AGTGTTGAGG TGCTCCCTAG TGATTCTGCA
    CCAGATCTAA AGCAAGAGGA GCGGCTGCAG GAACTGGAGA GCTGTTCAGG TTTGGGAAGC
    ACATCAGATG ACACTGAAGT TAGAGAGGTC AGCTCCCGGC CCAGCACTCC AGGACTCAGT
    GTGGTGTCAG GTATCAGTGC TACCTCAGAG GACATCCCAA ACAAGATAGA GGACCTACGC
    TCAGAGTGCA GCTCTGACTT TGGAGGAAAG GATTCTGTTA CAAGTCCTGA GGCAGAAGAG
    TCTGTTCATG GACCTCACCA TATAACTGCA CCTCCAACCC AGACTGAATC CTTGTTGGCC
    ATGTTTGACC CACTAGCTCC AGCATCCAAT GAAGTGGTTC GACCTAAAGT ACACTATGCG
    AGACCCTCAC ATCCGCCTCC TGACCCGCCT GTACTGGAAG GAGCAAGTGG GGGAAATGAA
    GCTCGTCTTC CTATGTTCGG TTCTCATGTA TTTGTTCCCA TGGAGTCTGA AGCTTACAGG
    CAGAGACACT CATGTCCTGA AAGACTGGTG CGCAGTCGCA GCTCCGACAT CGCTTCTTCC
    ATACGGAGGC CAATGAGTGA CCCTGGCTGG GTGCGGCGTG GGGTGAATGA AGATAGGGAG
    CTGCCAACAG GAACTGCAAT TAATGCACTA ATTAATCCCC CCCAGGCTTC ATCCTCCTCA
    CCAAGCAAGG ACTCTTCCAG GGGAGAGCCA GAAGAAAAAA AAGACAGCGA TGATGAGCGA
    TCAGATAGGA ATAAGCCTTG GTGGAAGAAG CGGTTTGTGT CTGCAATGCC CAAAGCTCCT
    ATCCCATTTA GGAAGAAGGA AAAACACGAG AAGGAGAGGG AAGACTTGGT ACAAGACAGG
    TTTTCAGTAG CAGATGACTC AACATCTCAG CTAGGGCCAC ATTCCCAGGC AGCAGAAGAC
    ATCCTGGCTA AGTACAGGAA TGCTATTAAG AGGACAATCC CCAATGAAGG TTCCTCCGTG
    CCCTATGAAG GAGCAGATGC TGTTGGAGAT GGAGAAAGCC TTCATGATTC TCCTCGTGAT
    GAAGCTTTGC AGAACATGGC CTCAGATGAC CTGCTTGATT CTGCCAGCCA AGTAGCCCAC
    CCACAGGAGC CTGCATTCTC CTATAGGGAT GCTAAGAAAA AATTACGCCT GGCCCTGTGC
    TCTGCGGATT CAGTAGCGTT CCCTGTATTG TCTCATTCGA CACGGAATGG CCTGCCAGAT
    CACACAGACC CAGAAGACAA TGAGATTGTG TGCTTCCTTA AAGTCCAACT TGCAGAGGCC
    ATAAACCTGC AGGACAAAAA CCTGATGGCT CAGATCCAGG AAACTATGCG ATGTGTGAGC
    CGATTTGACA GCCGCTCCTG CAGCAAAATT TTGGCATCCA TTGCAGAGGA TTACCGGAAG
    AGGGCCTCAT ATATTGCATA TCTAACAAGA TGCCGACAGG GACTACAGAC CACCCAGGCT
    CACCTTGAGC GCCTCCTGCA GAGAGTGCTA CGAGATAAAG AGGTCTCCAC GCGCTACTTC
    ACCACTGTCT GTGTGAGGCT TCTACTTGAG AGCAAAGAGA AGGAAATCCA TGAATTTATC
    CAAGATTTTC AAAAGTTAAC TGCAGCTGAT GATAAAACAG CACAAGTTGA AGAGTTCTTG
    CAATCACTTT ACGGGGCTAT GGCACAAGAT GTGATTTGGC AAAATGCCAG TGAGGAGCAG
    CTGCAAGATG CACAGATTGC TATTGAGCGA AGCATTATGA ACCGCATCTT TAAGCTGGCC
    TTCTATCCCA ATCAAGATGC AGATATTCTG CGAGATCAGG TCCTGCATGA ACACATTAAA
    AGGTTGTCTA AAGTGGTGAC TGCCAATCAC AGGGCACTGC AGATACCTGA GGTGTATCTG
    AGGGAGGCTC CTTGGCCATC TGCCCAAACC GAAATCCGTA CCATCAGCGC ATACAAGACA
    CCCAGGGACA AAGTGCAGTG CATATTGCGC ATGTGCTCAA CAATCATGAA CCTTCTAAGC
    CTGGCCAATG AGTACTCTGT CCCAGGAGCA GATGATTTTG TTCCTGTGCT GGTTTTTGTC
    CTTATTAAGG CTAATCCCCC CTGCCTACTA TCAACAGTGC AATATATCAG CAACTTCTAT
    TCCAATCGGC TCACTGGCGA GGAGTCCTAC TGGTGGATGC AGTTCACAGC TGCTGTTGAG
    TTCATTAAAA CCATTGACGA TCGCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31127
    Clone ID Related Accession (Same CDS sequence) XM_012969069.2 , XM_012969069.3
    Accession Version XM_012969069.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969069.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGAAGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTA TCAGTGCTAC CTCAGAGGAC
    ATCCCAAACA AGATAGAGGA CCTACGCTCA GAGTGCAGCT CTGACTTTGG AGGAAAGGAT
    TCTGTTACAA GTCCTGAGGC AGAAGAGTCT GTTCATGGAC CTCACCATAT AACTGCACCT
    CCAACCCAGA CTGAATCCTT GTTGGCCATG TTTGACCCAC TAGCTCCAGC ATCCAATGAA
    GTGGTTCGAC CTAAAGTACA CTATGCGAGA CCCTCACATC CGCCTCCTGA CCCGCCTGTA
    CTGGAAGGAG CAAGTGGGGG AAATGAAGCT CGTCTTCCTA TGTTCGGTTC TCATGTATTT
    GTTCCCATGG AGTCTGAAGC TTACAGGCAG AGACACTCAT GTCCTGAAAG ACTGGTGCGC
    AGTCGCAGCT CCGACATCGC TTCTTCCATA CGGAGGCCAA TGAGTGACCC TGGCTGGGTG
    CGGCGTGGGG TGAATGAAGA TAGGGAGCTG CCAACAGGAA CTGCAATTAA TGCACTAATT
    AATCCCCCCC AGGCTTCATC CTCCTCACCA AGCAAGGACT CTTCCAGGGG AGAGCCAGAA
    GAAAAAAAAG ACAGCGATGA TGAGCGATCA GATAGGAATA AGCCTTGGTG GAAGAAGCGG
    TTTGTGTCTG CAATGCCCAA AGCTCCTATC CCATTTAGGA AGAAGGAAAA ACACGAGAAG
    GAGAGGGAAG ACTTGGTACA AGACAGGTTT TCAGTAGCAG ATGACTCAAC ATCTCAGCTA
    GGGCCACATT CCCAGGCAGC AGAAGACATC CTGGCTAAGT ACAGGAATGC TATTAAGAGG
    ACAATCCCCA ATGAAGGTTC CTCCGTGCCC TATGAAGGAG CAGATGCTGT TGGAGATGGA
    GAAAGCCTTC ATGATTCTCC TCGTGATGAA GCTTTGCAGA ACATGGCCTC AGATGACCTG
    CTTGATTCTG CCAGCCAAGT AGCCCACCCA CAGGAGCCTG CATTCTCCTA TAGGGATGCT
    AAGAAAAAAT TACGCCTGGC CCTGTGCTCT GCGGATTCAG TAGCGTTCCC TGTATTGTCT
    CATTCGACAC GGAATGGCCT GCCAGATCAC ACAGACCCAG AAGACAATGA GATTGTGTGC
    TTCCTTAAAG TCCAACTTGC AGAGGCCATA AACCTGCAGG ACAAAAACCT GATGGCTCAG
    ATCCAGGAAA CTATGCGATG TGTGAGCCGA TTTGACAGCC GCTCCTGCAG CAAAATTTTG
    GCATCCATTG CAGAGGATTA CCGGAAGAGG GCCTCATATA TTGCATATCT AACAAGATGC
    CGACAGGGAC TACAGACCAC CCAGGCTCAC CTTGAGCGCC TCCTGCAGAG AGTGCTACGA
    GATAAAGAGG TCTCCACGCG CTACTTCACC ACTGTCTGTG TGAGGCTTCT ACTTGAGAGC
    AAAGAGAAGG AAATCCATGA ATTTATCCAA GATTTTCAAA AGTTAACTGC AGCTGATGAT
    AAAACAGCAC AAGTTGAAGA GTTCTTGCAA TCACTTTACG GGGCTATGGC ACAAGATGTG
    ATTTGGCAAA ATGCCAGTGA GGAGCAGCTG CAAGATGCAC AGATTGCTAT TGAGCGAAGC
    ATTATGAACC GCATCTTTAA GCTGGCCTTC TATCCCAATC AAGATGCAGA TATTCTGCGA
    GATCAGGTCC TGCATGAACA CATTAAAAGG TTGTCTAAAG TGGTGACTGC CAATCACAGG
    GCACTGCAGA TACCTGAGGT GTATCTGAGG GAGGCTCCTT GGCCATCTGC CCAAACCGAA
    ATCCGTACCA TCAGCGCATA CAAGACACCC AGGGACAAAG TGCAGTGCAT ATTGCGCATG
    TGCTCAACAA TCATGAACCT TCTAAGCCTG GCCAATGAGT ACTCTGTCCC AGGAGCAGAT
    GATTTTGTTC CTGTGCTGGT TTTTGTCCTT ATTAAGGCTA ATCCCCCCTG CCTACTATCA
    ACAGTGCAAT ATATCAGCAA CTTCTATTCC AATCGGCTCA CTGGCGAGGA GTCCTACTGG
    TGGATGCAGT TCACAGCTGC TGTTGAGTTC ATTAAAACCA TTGACGATCG CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824523.1
    CDS159..4595
    Translation

    Target ORF information:

    RefSeq Version XM_012969069.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969069.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGAAGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTA TCAGTGCTAC CTCAGAGGAC
    ATCCCAAACA AGATAGAGGA CCTACGCTCA GAGTGCAGCT CTGACTTTGG AGGAAAGGAT
    TCTGTTACAA GTCCTGAGGC AGAAGAGTCT GTTCATGGAC CTCACCATAT AACTGCACCT
    CCAACCCAGA CTGAATCCTT GTTGGCCATG TTTGACCCAC TAGCTCCAGC ATCCAATGAA
    GTGGTTCGAC CTAAAGTACA CTATGCGAGA CCCTCACATC CGCCTCCTGA CCCGCCTGTA
    CTGGAAGGAG CAAGTGGGGG AAATGAAGCT CGTCTTCCTA TGTTCGGTTC TCATGTATTT
    GTTCCCATGG AGTCTGAAGC TTACAGGCAG AGACACTCAT GTCCTGAAAG ACTGGTGCGC
    AGTCGCAGCT CCGACATCGC TTCTTCCATA CGGAGGCCAA TGAGTGACCC TGGCTGGGTG
    CGGCGTGGGG TGAATGAAGA TAGGGAGCTG CCAACAGGAA CTGCAATTAA TGCACTAATT
    AATCCCCCCC AGGCTTCATC CTCCTCACCA AGCAAGGACT CTTCCAGGGG AGAGCCAGAA
    GAAAAAAAAG ACAGCGATGA TGAGCGATCA GATAGGAATA AGCCTTGGTG GAAGAAGCGG
    TTTGTGTCTG CAATGCCCAA AGCTCCTATC CCATTTAGGA AGAAGGAAAA ACACGAGAAG
    GAGAGGGAAG ACTTGGTACA AGACAGGTTT TCAGTAGCAG ATGACTCAAC ATCTCAGCTA
    GGGCCACATT CCCAGGCAGC AGAAGACATC CTGGCTAAGT ACAGGAATGC TATTAAGAGG
    ACAATCCCCA ATGAAGGTTC CTCCGTGCCC TATGAAGGAG CAGATGCTGT TGGAGATGGA
    GAAAGCCTTC ATGATTCTCC TCGTGATGAA GCTTTGCAGA ACATGGCCTC AGATGACCTG
    CTTGATTCTG CCAGCCAAGT AGCCCACCCA CAGGAGCCTG CATTCTCCTA TAGGGATGCT
    AAGAAAAAAT TACGCCTGGC CCTGTGCTCT GCGGATTCAG TAGCGTTCCC TGTATTGTCT
    CATTCGACAC GGAATGGCCT GCCAGATCAC ACAGACCCAG AAGACAATGA GATTGTGTGC
    TTCCTTAAAG TCCAACTTGC AGAGGCCATA AACCTGCAGG ACAAAAACCT GATGGCTCAG
    ATCCAGGAAA CTATGCGATG TGTGAGCCGA TTTGACAGCC GCTCCTGCAG CAAAATTTTG
    GCATCCATTG CAGAGGATTA CCGGAAGAGG GCCTCATATA TTGCATATCT AACAAGATGC
    CGACAGGGAC TACAGACCAC CCAGGCTCAC CTTGAGCGCC TCCTGCAGAG AGTGCTACGA
    GATAAAGAGG TCTCCACGCG CTACTTCACC ACTGTCTGTG TGAGGCTTCT ACTTGAGAGC
    AAAGAGAAGG AAATCCATGA ATTTATCCAA GATTTTCAAA AGTTAACTGC AGCTGATGAT
    AAAACAGCAC AAGTTGAAGA GTTCTTGCAA TCACTTTACG GGGCTATGGC ACAAGATGTG
    ATTTGGCAAA ATGCCAGTGA GGAGCAGCTG CAAGATGCAC AGATTGCTAT TGAGCGAAGC
    ATTATGAACC GCATCTTTAA GCTGGCCTTC TATCCCAATC AAGATGCAGA TATTCTGCGA
    GATCAGGTCC TGCATGAACA CATTAAAAGG TTGTCTAAAG TGGTGACTGC CAATCACAGG
    GCACTGCAGA TACCTGAGGT GTATCTGAGG GAGGCTCCTT GGCCATCTGC CCAAACCGAA
    ATCCGTACCA TCAGCGCATA CAAGACACCC AGGGACAAAG TGCAGTGCAT ATTGCGCATG
    TGCTCAACAA TCATGAACCT TCTAAGCCTG GCCAATGAGT ACTCTGTCCC AGGAGCAGAT
    GATTTTGTTC CTGTGCTGGT TTTTGTCCTT ATTAAGGCTA ATCCCCCCTG CCTACTATCA
    ACAGTGCAAT ATATCAGCAA CTTCTATTCC AATCGGCTCA CTGGCGAGGA GTCCTACTGG
    TGGATGCAGT TCACAGCTGC TGTTGAGTTC ATTAAAACCA TTGACGATCG CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31128
    Clone ID Related Accession (Same CDS sequence) XM_031891625.1
    Accession Version XM_031891625.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG GTGAATGTTT GGGTTTAAGA
    ATAATTGACG TTCCAGGTCC TTCAAATCGT TCCAACTCAG TTTCCTCTTT GGATCTGGAA
    GGGGAGTCTG TATCTGAGCT AGGAGGAGGA CCATCCGGAA GCAATGGGGT GGAGGCTTTG
    CAGTTATTGG AACATGAACA AGCTACCACA CAGGATAATC TAGATGACAA ACTGCGCAAA
    TTTGAAATTC GAGACATGAT GGGATTGACG GATGATAGAG ATATATCAGA GACAGTCAGT
    GAGACATGGA GCACGGATGT GCTTGGAAGT GATTTTGACC CCAACATTGA TGAAGATCGA
    CTACAAGAAA TTGCAGGTGC AGCAGCTGAG AACATGCTGG GAAGCCTGCT GTGCCTTCCC
    GGCTCTGGCT CAATACTGCT TGATCCTTGT ACTGGATCCA CTATATCTGA GACTACCAGT
    GAAGCATGGA GTGTTGAGGT GCTCCCTAGT GATTCTGAAG CACCAGATCT AAAGCAAGAG
    GAGCGGCTGC AGGAACTGGA GAGCTGTTCA GGTTTGGGAA GCACATCAGA TGACACTGAA
    GTTAGAGAGG TCAGCTCCCG GCCCAGCACT CCAGGACTCA GTGTGGTGTC AGGTGACTTT
    CCCAAACCTT TCTATTCATT GCCGGGTATC AGTGCTACCT CAGAGGACAT CCCAAACAAG
    ATAGAGGACC TACGCTCAGA GTGCAGCTCT GACTTTGGAG GAAAGGATTC TGTTACAAGT
    CCTGAGGCAG AAGAGTCTGT TCATGGACCT CACCATATAA CTGCACCTCC AACCCAGACT
    GAATCCTTGT TGGCCATGTT TGACCCACTA GCTCCAGCAT CCAATGAAGT GGTTCGACCT
    AAAGTACACT ATGCGAGACC CTCACATCCG CCTCCTGACC CGCCTGTACT GGAAGGAGCA
    AGTGGGGGAA ATGAAGCTCG TCTTCCTATG TTCGGTTCTC ATGTATTTGT TCCCATGGAG
    TCTGAAGCTT ACAGGCAGAG ACACTCATGT CCTGAAAGAC TGGTGCGCAG TCGCAGCTCC
    GACATCGCTT CTTCCATACG GAGGCCAATG AGTGACCCTG GCTGGGTGCG GCGTGGGGTG
    AATGAAGATA GGGAGCTGCC AACAGGAACT GCAATTAATG CACTAATTAA TCCCCCCCAG
    GCTTCATCCT CCTCACCAAG CAAGGACTCT TCCAGGGGAG AGCCAGAAGA AAAAAAAGAC
    AGCGATGATG AGCGATCAGA TAGGAATAAG CCTTGGTGGA AGAAGCGGTT TGTGTCTGCA
    ATGCCCAAAG CTCCTATCCC ATTTAGGAAG AAGGAAAAAC ACGAGAAGGA GAGGGAAGAC
    TTGGTACAAG ACAGGTTTTC AGTAGCAGAT GACTCAACAT CTCAGCTAGG GCCACATTCC
    CAGGCAGCAG AAGACATCCT GGCTAAGTAC AGGAATGCTA TTAAGAGGAC AATCCCCAAT
    GAAGGTTCCT CCGTGCCCTA TGAAGGAGCA GATGCTGTTG GAGATGGAGA AAGCCTTCAT
    GATTCTCCTC GTGATGAAGC TTTGCAGAAC ATGGCCTCAG ATGACCTGCT TGATTCTGCC
    AGCCAAGTAG CCCACCCACA GGAGCCTGCA TTCTCCTATA GGGATGCTAA GAAAAAATTA
    CGCCTGGCCC TGTGCTCTGC GGATTCAGTA GCGTTCCCTG TATTGTCTCA TTCGACACGG
    AATGGCCTGC CAGATCACAC AGACCCAGAA GACAATGAGA TTGTGTGCTT CCTTAAAGTC
    CAACTTGCAG AGGCCATAAA CCTGCAGGAC AAAAACCTGA TGGCTCAGAT CCAGGAAACT
    ATGCGATGTG TGAGCCGATT TGACAGCCGC TCCTGCAGCA AAATTTTGGC ATCCATTGCA
    GAGGATTACC GGAAGAGGGC CTCATATATT GCATATCTAA CAAGATGCCG ACAGGGACTA
    CAGACCACCC AGGCTCACCT TGAGCGCCTC CTGCAGAGAG TGCTACGAGA TAAAGAGGTC
    TCCACGCGCT ACTTCACCAC TGTCTGTGTG AGGCTTCTAC TTGAGAGCAA AGAGAAGGAA
    ATCCATGAAT TTATCCAAGA TTTTCAAAAG TTAACTGCAG CTGATGATAA AACAGCACAA
    GTTGAAGAGT TCTTGCAATC ACTTTACGGG GCTATGGCAC AAGATGTGAT TTGGCAAAAT
    GCCAGTGAGG AGCAGCTGCA AGATGCACAG ATTGCTATTG AGCGAAGCAT TATGAACCGC
    ATCTTTAAGC TGGCCTTCTA TCCCAATCAA GATGCAGATA TTCTGCGAGA TCAGGTCCTG
    CATGAACACA TTAAAAGGTT GTCTAAAGTG GTGACTGCCA ATCACAGGGC ACTGCAGATA
    CCTGAGGTGT ATCTGAGGGA GGCTCCTTGG CCATCTGCCC AAACCGAAAT CCGTACCATC
    AGCGCATACA AGACACCCAG GGACAAAGTG CAGTGCATAT TGCGCATGTG CTCAACAATC
    ATGAACCTTC TAAGCCTGGC CAATGAGTAC TCTGTCCCAG GAGCAGATGA TTTTGTTCCT
    GTGCTGGTTT TTGTCCTTAT TAAGGCTAAT CCCCCCTGCC TACTATCAAC AGTGCAATAT
    ATCAGCAACT TCTATTCCAA TCGGCTCACT GGCGAGGAGT CCTACTGGTG GATGCAGTTC
    ACAGCTGCTG TTGAGTTCAT TAAAACCATT GACGATCGCA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031747485.1
    CDS160..4584
    Translation

    Target ORF information:

    RefSeq Version XM_031891625.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031891625.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG GTGAATGTTT GGGTTTAAGA
    ATAATTGACG TTCCAGGTCC TTCAAATCGT TCCAACTCAG TTTCCTCTTT GGATCTGGAA
    GGGGAGTCTG TATCTGAGCT AGGAGGAGGA CCATCCGGAA GCAATGGGGT GGAGGCTTTG
    CAGTTATTGG AACATGAACA AGCTACCACA CAGGATAATC TAGATGACAA ACTGCGCAAA
    TTTGAAATTC GAGACATGAT GGGATTGACG GATGATAGAG ATATATCAGA GACAGTCAGT
    GAGACATGGA GCACGGATGT GCTTGGAAGT GATTTTGACC CCAACATTGA TGAAGATCGA
    CTACAAGAAA TTGCAGGTGC AGCAGCTGAG AACATGCTGG GAAGCCTGCT GTGCCTTCCC
    GGCTCTGGCT CAATACTGCT TGATCCTTGT ACTGGATCCA CTATATCTGA GACTACCAGT
    GAAGCATGGA GTGTTGAGGT GCTCCCTAGT GATTCTGAAG CACCAGATCT AAAGCAAGAG
    GAGCGGCTGC AGGAACTGGA GAGCTGTTCA GGTTTGGGAA GCACATCAGA TGACACTGAA
    GTTAGAGAGG TCAGCTCCCG GCCCAGCACT CCAGGACTCA GTGTGGTGTC AGGTGACTTT
    CCCAAACCTT TCTATTCATT GCCGGGTATC AGTGCTACCT CAGAGGACAT CCCAAACAAG
    ATAGAGGACC TACGCTCAGA GTGCAGCTCT GACTTTGGAG GAAAGGATTC TGTTACAAGT
    CCTGAGGCAG AAGAGTCTGT TCATGGACCT CACCATATAA CTGCACCTCC AACCCAGACT
    GAATCCTTGT TGGCCATGTT TGACCCACTA GCTCCAGCAT CCAATGAAGT GGTTCGACCT
    AAAGTACACT ATGCGAGACC CTCACATCCG CCTCCTGACC CGCCTGTACT GGAAGGAGCA
    AGTGGGGGAA ATGAAGCTCG TCTTCCTATG TTCGGTTCTC ATGTATTTGT TCCCATGGAG
    TCTGAAGCTT ACAGGCAGAG ACACTCATGT CCTGAAAGAC TGGTGCGCAG TCGCAGCTCC
    GACATCGCTT CTTCCATACG GAGGCCAATG AGTGACCCTG GCTGGGTGCG GCGTGGGGTG
    AATGAAGATA GGGAGCTGCC AACAGGAACT GCAATTAATG CACTAATTAA TCCCCCCCAG
    GCTTCATCCT CCTCACCAAG CAAGGACTCT TCCAGGGGAG AGCCAGAAGA AAAAAAAGAC
    AGCGATGATG AGCGATCAGA TAGGAATAAG CCTTGGTGGA AGAAGCGGTT TGTGTCTGCA
    ATGCCCAAAG CTCCTATCCC ATTTAGGAAG AAGGAAAAAC ACGAGAAGGA GAGGGAAGAC
    TTGGTACAAG ACAGGTTTTC AGTAGCAGAT GACTCAACAT CTCAGCTAGG GCCACATTCC
    CAGGCAGCAG AAGACATCCT GGCTAAGTAC AGGAATGCTA TTAAGAGGAC AATCCCCAAT
    GAAGGTTCCT CCGTGCCCTA TGAAGGAGCA GATGCTGTTG GAGATGGAGA AAGCCTTCAT
    GATTCTCCTC GTGATGAAGC TTTGCAGAAC ATGGCCTCAG ATGACCTGCT TGATTCTGCC
    AGCCAAGTAG CCCACCCACA GGAGCCTGCA TTCTCCTATA GGGATGCTAA GAAAAAATTA
    CGCCTGGCCC TGTGCTCTGC GGATTCAGTA GCGTTCCCTG TATTGTCTCA TTCGACACGG
    AATGGCCTGC CAGATCACAC AGACCCAGAA GACAATGAGA TTGTGTGCTT CCTTAAAGTC
    CAACTTGCAG AGGCCATAAA CCTGCAGGAC AAAAACCTGA TGGCTCAGAT CCAGGAAACT
    ATGCGATGTG TGAGCCGATT TGACAGCCGC TCCTGCAGCA AAATTTTGGC ATCCATTGCA
    GAGGATTACC GGAAGAGGGC CTCATATATT GCATATCTAA CAAGATGCCG ACAGGGACTA
    CAGACCACCC AGGCTCACCT TGAGCGCCTC CTGCAGAGAG TGCTACGAGA TAAAGAGGTC
    TCCACGCGCT ACTTCACCAC TGTCTGTGTG AGGCTTCTAC TTGAGAGCAA AGAGAAGGAA
    ATCCATGAAT TTATCCAAGA TTTTCAAAAG TTAACTGCAG CTGATGATAA AACAGCACAA
    GTTGAAGAGT TCTTGCAATC ACTTTACGGG GCTATGGCAC AAGATGTGAT TTGGCAAAAT
    GCCAGTGAGG AGCAGCTGCA AGATGCACAG ATTGCTATTG AGCGAAGCAT TATGAACCGC
    ATCTTTAAGC TGGCCTTCTA TCCCAATCAA GATGCAGATA TTCTGCGAGA TCAGGTCCTG
    CATGAACACA TTAAAAGGTT GTCTAAAGTG GTGACTGCCA ATCACAGGGC ACTGCAGATA
    CCTGAGGTGT ATCTGAGGGA GGCTCCTTGG CCATCTGCCC AAACCGAAAT CCGTACCATC
    AGCGCATACA AGACACCCAG GGACAAAGTG CAGTGCATAT TGCGCATGTG CTCAACAATC
    ATGAACCTTC TAAGCCTGGC CAATGAGTAC TCTGTCCCAG GAGCAGATGA TTTTGTTCCT
    GTGCTGGTTT TTGTCCTTAT TAAGGCTAAT CCCCCCTGCC TACTATCAAC AGTGCAATAT
    ATCAGCAACT TCTATTCCAA TCGGCTCACT GGCGAGGAGT CCTACTGGTG GATGCAGTTC
    ACAGCTGCTG TTGAGTTCAT TAAAACCATT GACGATCGCA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46317
    Clone ID Related Accession (Same CDS sequence) XM_018096655.1
    Accession Version XM_018096655.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGAAG AGGAAAATCC GTGTTCAACA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGAAGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTA TCAGTGCTAC CTCAGAGGAC
    ATCCCAAACA AGATAGAGGA CCTACGCTCA GAGTGCAGCT CTGACTTTGG AGGAAAGGAT
    TCTGTTACAA GTCCTGAGGC AGAAGAGTCT GTTCATGGAC CTCACCATAT AACTGCACCT
    CCAACCCAGA CTGAATCCTT GTTGGCCATG TTTGACCCAC TAGCTCCAGC ATCCAATGAA
    GTGGTTCGAC CTAAAGTACA CTATGCGAGA CCCTCACATC CGCCTCCTGA CCCGCCTGTA
    CTGGAAGGAG CAAGTGGGGG AAATGAAGCT CGTCTTCCTA TGTTCGGTTC TCATGTATTT
    GTTCCCATGG AGTCTGAAGC TTACAGGCAG AGACACTCAT GTCCTGAAAG ACTGGTGCGC
    AGTCGCAGCT CCGACATCGC TTCTTCCATA CGGAGGCCAA TGAGTGACCC TGGCTGGGTG
    CGGCGTGGGG TGAATGAAGA TAGGGAGCTG CCAACAGGAA CTGCAATTAA TGCACTAATT
    AATCCCCCCC AGGCTTCATC CTCCTCACCA AGCAAGGACT CTTCCAGGGG AGAGCCAGAA
    GAAAAAAAAG ACAGCGATGA TGAGCGATCA GATAGGAATA AGCCTTGGTG GAAGAAGCGG
    TTTGTGTCTG CAATGCCCAA AGCTCCTATC CCATTTAGGA AGAAGGAAAA ACACGAGAAG
    GAGAGGGAAG ACTTGGTACA AGACAGGTTT TCAGTAGCAG ATGACTCAAC ATCTCAGCTA
    GGGCCACATT CCCAGGCAGC AGAAGACATC CTGGCTAAGT ACAGGAATGC TATTAAGAGG
    ACAATCCCCA ATGAAGGTTC CTCCGTGCCC TATGAAGGAG CAGATGCTGT TGGAGATGGA
    GAAAGCCTTC ATGATTCTCC TCGTGATGAA GCTTTGCAGA ACATGGCCTC AGATGACCTG
    CTTGATTCTG CCAGCCAAGT AGCCCACCCA CAGGAGCCTG CATTCTCCTA TAGGGATGCT
    AAGAAAAAAT TACGCCTGGC CCTGTGCTCT GCGGATTCAG TAGCGTTCCC TGTATTGTCT
    CATTCGACAC GGAATGGCCT GCCAGATCAC ACAGACCCAG AAGACAATGA GATTGTGTGC
    TTCCTTAAAG TCCAACTTGC AGAGGCCATA AACCTGCAGG ACAAAAACCT GATGGCTCAG
    ATCCAGGAAA CTATGCGATG TGTGAGCCGA TTTGACAGCC GCTCCTGCAG CAAAATTTTG
    GCATCCATTG CAGAGGATTA CCGGAAGAGG GCCTCATATA TTGCATATCT AACAAGATGC
    CGACAGGGAC TACAGACCAC CCAGGCTCAC CTTGAGCGCC TCCTGCAGAG AGTGCTACGA
    GATAAAGAGG TCTCCACGCG CTACTTCACC ACTGTCTGTG TGAGGCTTCT ACTTGAGAGC
    AAAGAGAAGG AAATCCATGA ATTTATCCAA GATTTTCAAA AGTTAACTGC AGCTGATGAT
    AAAACAGCAC AAGTTGAAGA GTTCTTGCAA TCACTTTACG GGGCTATGGC ACAAGATGTG
    ATTTGGCAAA ATGCCAGTGA GGAGCAGCTG CAAGATGCAC AGATTGCTAT TGAGCGAAGC
    ATTATGAACC GCATCTTTAA GCTGGCCTTC TATCCCAATC AAGATGCAGA TATTCTGCGA
    GATCAGGTCC TGCATGAACA CATTAAAAGG TTGTCTAAAG TGGTGACTGC CAATCACAGG
    GCACTGCAGA TACCTGAGGT GTATCTGAGG GAGGCTCCTT GGCCATCTGC CCAAACCGAA
    ATCCGTACCA TCAGCGCATA CAAGACACCC AGGGACAAAG TGCAGTGCAT ATTGCGCATG
    TGCTCAACAA TCATGAACCT TCTAAGCCTG GCCAATGAGT ACTCTGTCCC AGGAGCAGAT
    GATTTTGTTC CTGTGCTGGT TTTTGTCCTT ATTAAGGCTA ATCCCCCCTG CCTACTATCA
    ACAGTGCAAT ATATCAGCAA CTTCTATTCC AATCGGCTCA CTGGCGAGGA GTCCTACTGG
    TGGATGCAGT TCACAGCTGC TGTTGAGTTC ATTAAAACCA TTGACGATCG CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952144.1
    CDS169..4665
    Translation

    Target ORF information:

    RefSeq Version XM_018096655.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096655.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGAAG AGGAAAATCC GTGTTCAACA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGAAGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTA TCAGTGCTAC CTCAGAGGAC
    ATCCCAAACA AGATAGAGGA CCTACGCTCA GAGTGCAGCT CTGACTTTGG AGGAAAGGAT
    TCTGTTACAA GTCCTGAGGC AGAAGAGTCT GTTCATGGAC CTCACCATAT AACTGCACCT
    CCAACCCAGA CTGAATCCTT GTTGGCCATG TTTGACCCAC TAGCTCCAGC ATCCAATGAA
    GTGGTTCGAC CTAAAGTACA CTATGCGAGA CCCTCACATC CGCCTCCTGA CCCGCCTGTA
    CTGGAAGGAG CAAGTGGGGG AAATGAAGCT CGTCTTCCTA TGTTCGGTTC TCATGTATTT
    GTTCCCATGG AGTCTGAAGC TTACAGGCAG AGACACTCAT GTCCTGAAAG ACTGGTGCGC
    AGTCGCAGCT CCGACATCGC TTCTTCCATA CGGAGGCCAA TGAGTGACCC TGGCTGGGTG
    CGGCGTGGGG TGAATGAAGA TAGGGAGCTG CCAACAGGAA CTGCAATTAA TGCACTAATT
    AATCCCCCCC AGGCTTCATC CTCCTCACCA AGCAAGGACT CTTCCAGGGG AGAGCCAGAA
    GAAAAAAAAG ACAGCGATGA TGAGCGATCA GATAGGAATA AGCCTTGGTG GAAGAAGCGG
    TTTGTGTCTG CAATGCCCAA AGCTCCTATC CCATTTAGGA AGAAGGAAAA ACACGAGAAG
    GAGAGGGAAG ACTTGGTACA AGACAGGTTT TCAGTAGCAG ATGACTCAAC ATCTCAGCTA
    GGGCCACATT CCCAGGCAGC AGAAGACATC CTGGCTAAGT ACAGGAATGC TATTAAGAGG
    ACAATCCCCA ATGAAGGTTC CTCCGTGCCC TATGAAGGAG CAGATGCTGT TGGAGATGGA
    GAAAGCCTTC ATGATTCTCC TCGTGATGAA GCTTTGCAGA ACATGGCCTC AGATGACCTG
    CTTGATTCTG CCAGCCAAGT AGCCCACCCA CAGGAGCCTG CATTCTCCTA TAGGGATGCT
    AAGAAAAAAT TACGCCTGGC CCTGTGCTCT GCGGATTCAG TAGCGTTCCC TGTATTGTCT
    CATTCGACAC GGAATGGCCT GCCAGATCAC ACAGACCCAG AAGACAATGA GATTGTGTGC
    TTCCTTAAAG TCCAACTTGC AGAGGCCATA AACCTGCAGG ACAAAAACCT GATGGCTCAG
    ATCCAGGAAA CTATGCGATG TGTGAGCCGA TTTGACAGCC GCTCCTGCAG CAAAATTTTG
    GCATCCATTG CAGAGGATTA CCGGAAGAGG GCCTCATATA TTGCATATCT AACAAGATGC
    CGACAGGGAC TACAGACCAC CCAGGCTCAC CTTGAGCGCC TCCTGCAGAG AGTGCTACGA
    GATAAAGAGG TCTCCACGCG CTACTTCACC ACTGTCTGTG TGAGGCTTCT ACTTGAGAGC
    AAAGAGAAGG AAATCCATGA ATTTATCCAA GATTTTCAAA AGTTAACTGC AGCTGATGAT
    AAAACAGCAC AAGTTGAAGA GTTCTTGCAA TCACTTTACG GGGCTATGGC ACAAGATGTG
    ATTTGGCAAA ATGCCAGTGA GGAGCAGCTG CAAGATGCAC AGATTGCTAT TGAGCGAAGC
    ATTATGAACC GCATCTTTAA GCTGGCCTTC TATCCCAATC AAGATGCAGA TATTCTGCGA
    GATCAGGTCC TGCATGAACA CATTAAAAGG TTGTCTAAAG TGGTGACTGC CAATCACAGG
    GCACTGCAGA TACCTGAGGT GTATCTGAGG GAGGCTCCTT GGCCATCTGC CCAAACCGAA
    ATCCGTACCA TCAGCGCATA CAAGACACCC AGGGACAAAG TGCAGTGCAT ATTGCGCATG
    TGCTCAACAA TCATGAACCT TCTAAGCCTG GCCAATGAGT ACTCTGTCCC AGGAGCAGAT
    GATTTTGTTC CTGTGCTGGT TTTTGTCCTT ATTAAGGCTA ATCCCCCCTG CCTACTATCA
    ACAGTGCAAT ATATCAGCAA CTTCTATTCC AATCGGCTCA CTGGCGAGGA GTCCTACTGG
    TGGATGCAGT TCACAGCTGC TGTTGAGTTC ATTAAAACCA TTGACGATCG CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46318
    Clone ID Related Accession (Same CDS sequence) XM_018096656.1
    Accession Version XM_018096656.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGAAG AGGAAAATCC GTGTTCAACA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGCACCAGAT CTAAAGCAAG AGGAGCGGCT GCAGGAACTG
    GAGAGCTGTT CAGGTTTGGG AAGCACATCA GATGACACTG AAGTTAGAGA GGTCAGCTCC
    CGGCCCAGCA CTCCAGGACT CAGTGTGGTG TCAGGTATCA GTGCTACCTC AGAGGACATC
    CCAAACAAGA TAGAGGACCT ACGCTCAGAG TGCAGCTCTG ACTTTGGAGG AAAGGATTCT
    GTTACAAGTC CTGAGGCAGA AGAGTCTGTT CATGGACCTC ACCATATAAC TGCACCTCCA
    ACCCAGACTG AATCCTTGTT GGCCATGTTT GACCCACTAG CTCCAGCATC CAATGAAGTG
    GTTCGACCTA AAGTACACTA TGCGAGACCC TCACATCCGC CTCCTGACCC GCCTGTACTG
    GAAGGAGCAA GTGGGGGAAA TGAAGCTCGT CTTCCTATGT TCGGTTCTCA TGTATTTGTT
    CCCATGGAGT CTGAAGCTTA CAGGCAGAGA CACTCATGTC CTGAAAGACT GGTGCGCAGT
    CGCAGCTCCG ACATCGCTTC TTCCATACGG AGGCCAATGA GTGACCCTGG CTGGGTGCGG
    CGTGGGGTGA ATGAAGATAG GGAGCTGCCA ACAGGAACTG CAATTAATGC ACTAATTAAT
    CCCCCCCAGG CTTCATCCTC CTCACCAAGC AAGGACTCTT CCAGGGGAGA GCCAGAAGAA
    AAAAAAGACA GCGATGATGA GCGATCAGAT AGGAATAAGC CTTGGTGGAA GAAGCGGTTT
    GTGTCTGCAA TGCCCAAAGC TCCTATCCCA TTTAGGAAGA AGGAAAAACA CGAGAAGGAG
    AGGGAAGACT TGGTACAAGA CAGGTTTTCA GTAGCAGATG ACTCAACATC TCAGCTAGGG
    CCACATTCCC AGGCAGCAGA AGACATCCTG GCTAAGTACA GGAATGCTAT TAAGAGGACA
    ATCCCCAATG AAGGTTCCTC CGTGCCCTAT GAAGGAGCAG ATGCTGTTGG AGATGGAGAA
    AGCCTTCATG ATTCTCCTCG TGATGAAGCT TTGCAGAACA TGGCCTCAGA TGACCTGCTT
    GATTCTGCCA GCCAAGTAGC CCACCCACAG GAGCCTGCAT TCTCCTATAG GGATGCTAAG
    AAAAAATTAC GCCTGGCCCT GTGCTCTGCG GATTCAGTAG CGTTCCCTGT ATTGTCTCAT
    TCGACACGGA ATGGCCTGCC AGATCACACA GACCCAGAAG ACAATGAGAT TGTGTGCTTC
    CTTAAAGTCC AACTTGCAGA GGCCATAAAC CTGCAGGACA AAAACCTGAT GGCTCAGATC
    CAGGAAACTA TGCGATGTGT GAGCCGATTT GACAGCCGCT CCTGCAGCAA AATTTTGGCA
    TCCATTGCAG AGGATTACCG GAAGAGGGCC TCATATATTG CATATCTAAC AAGATGCCGA
    CAGGGACTAC AGACCACCCA GGCTCACCTT GAGCGCCTCC TGCAGAGAGT GCTACGAGAT
    AAAGAGGTCT CCACGCGCTA CTTCACCACT GTCTGTGTGA GGCTTCTACT TGAGAGCAAA
    GAGAAGGAAA TCCATGAATT TATCCAAGAT TTTCAAAAGT TAACTGCAGC TGATGATAAA
    ACAGCACAAG TTGAAGAGTT CTTGCAATCA CTTTACGGGG CTATGGCACA AGATGTGATT
    TGGCAAAATG CCAGTGAGGA GCAGCTGCAA GATGCACAGA TTGCTATTGA GCGAAGCATT
    ATGAACCGCA TCTTTAAGCT GGCCTTCTAT CCCAATCAAG ATGCAGATAT TCTGCGAGAT
    CAGGTCCTGC ATGAACACAT TAAAAGGTTG TCTAAAGTGG TGACTGCCAA TCACAGGGCA
    CTGCAGATAC CTGAGGTGTA TCTGAGGGAG GCTCCTTGGC CATCTGCCCA AACCGAAATC
    CGTACCATCA GCGCATACAA GACACCCAGG GACAAAGTGC AGTGCATATT GCGCATGTGC
    TCAACAATCA TGAACCTTCT AAGCCTGGCC AATGAGTACT CTGTCCCAGG AGCAGATGAT
    TTTGTTCCTG TGCTGGTTTT TGTCCTTATT AAGGCTAATC CCCCCTGCCT ACTATCAACA
    GTGCAATATA TCAGCAACTT CTATTCCAAT CGGCTCACTG GCGAGGAGTC CTACTGGTGG
    ATGCAGTTCA CAGCTGCTGT TGAGTTCATT AAAACCATTG ACGATCGCAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952145.1
    CDS170..4663
    Translation

    Target ORF information:

    RefSeq Version XM_018096656.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096656.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGAAG AGGAAAATCC GTGTTCAACA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGCACCAGAT CTAAAGCAAG AGGAGCGGCT GCAGGAACTG
    GAGAGCTGTT CAGGTTTGGG AAGCACATCA GATGACACTG AAGTTAGAGA GGTCAGCTCC
    CGGCCCAGCA CTCCAGGACT CAGTGTGGTG TCAGGTATCA GTGCTACCTC AGAGGACATC
    CCAAACAAGA TAGAGGACCT ACGCTCAGAG TGCAGCTCTG ACTTTGGAGG AAAGGATTCT
    GTTACAAGTC CTGAGGCAGA AGAGTCTGTT CATGGACCTC ACCATATAAC TGCACCTCCA
    ACCCAGACTG AATCCTTGTT GGCCATGTTT GACCCACTAG CTCCAGCATC CAATGAAGTG
    GTTCGACCTA AAGTACACTA TGCGAGACCC TCACATCCGC CTCCTGACCC GCCTGTACTG
    GAAGGAGCAA GTGGGGGAAA TGAAGCTCGT CTTCCTATGT TCGGTTCTCA TGTATTTGTT
    CCCATGGAGT CTGAAGCTTA CAGGCAGAGA CACTCATGTC CTGAAAGACT GGTGCGCAGT
    CGCAGCTCCG ACATCGCTTC TTCCATACGG AGGCCAATGA GTGACCCTGG CTGGGTGCGG
    CGTGGGGTGA ATGAAGATAG GGAGCTGCCA ACAGGAACTG CAATTAATGC ACTAATTAAT
    CCCCCCCAGG CTTCATCCTC CTCACCAAGC AAGGACTCTT CCAGGGGAGA GCCAGAAGAA
    AAAAAAGACA GCGATGATGA GCGATCAGAT AGGAATAAGC CTTGGTGGAA GAAGCGGTTT
    GTGTCTGCAA TGCCCAAAGC TCCTATCCCA TTTAGGAAGA AGGAAAAACA CGAGAAGGAG
    AGGGAAGACT TGGTACAAGA CAGGTTTTCA GTAGCAGATG ACTCAACATC TCAGCTAGGG
    CCACATTCCC AGGCAGCAGA AGACATCCTG GCTAAGTACA GGAATGCTAT TAAGAGGACA
    ATCCCCAATG AAGGTTCCTC CGTGCCCTAT GAAGGAGCAG ATGCTGTTGG AGATGGAGAA
    AGCCTTCATG ATTCTCCTCG TGATGAAGCT TTGCAGAACA TGGCCTCAGA TGACCTGCTT
    GATTCTGCCA GCCAAGTAGC CCACCCACAG GAGCCTGCAT TCTCCTATAG GGATGCTAAG
    AAAAAATTAC GCCTGGCCCT GTGCTCTGCG GATTCAGTAG CGTTCCCTGT ATTGTCTCAT
    TCGACACGGA ATGGCCTGCC AGATCACACA GACCCAGAAG ACAATGAGAT TGTGTGCTTC
    CTTAAAGTCC AACTTGCAGA GGCCATAAAC CTGCAGGACA AAAACCTGAT GGCTCAGATC
    CAGGAAACTA TGCGATGTGT GAGCCGATTT GACAGCCGCT CCTGCAGCAA AATTTTGGCA
    TCCATTGCAG AGGATTACCG GAAGAGGGCC TCATATATTG CATATCTAAC AAGATGCCGA
    CAGGGACTAC AGACCACCCA GGCTCACCTT GAGCGCCTCC TGCAGAGAGT GCTACGAGAT
    AAAGAGGTCT CCACGCGCTA CTTCACCACT GTCTGTGTGA GGCTTCTACT TGAGAGCAAA
    GAGAAGGAAA TCCATGAATT TATCCAAGAT TTTCAAAAGT TAACTGCAGC TGATGATAAA
    ACAGCACAAG TTGAAGAGTT CTTGCAATCA CTTTACGGGG CTATGGCACA AGATGTGATT
    TGGCAAAATG CCAGTGAGGA GCAGCTGCAA GATGCACAGA TTGCTATTGA GCGAAGCATT
    ATGAACCGCA TCTTTAAGCT GGCCTTCTAT CCCAATCAAG ATGCAGATAT TCTGCGAGAT
    CAGGTCCTGC ATGAACACAT TAAAAGGTTG TCTAAAGTGG TGACTGCCAA TCACAGGGCA
    CTGCAGATAC CTGAGGTGTA TCTGAGGGAG GCTCCTTGGC CATCTGCCCA AACCGAAATC
    CGTACCATCA GCGCATACAA GACACCCAGG GACAAAGTGC AGTGCATATT GCGCATGTGC
    TCAACAATCA TGAACCTTCT AAGCCTGGCC AATGAGTACT CTGTCCCAGG AGCAGATGAT
    TTTGTTCCTG TGCTGGTTTT TGTCCTTATT AAGGCTAATC CCCCCTGCCT ACTATCAACA
    GTGCAATATA TCAGCAACTT CTATTCCAAT CGGCTCACTG GCGAGGAGTC CTACTGGTGG
    ATGCAGTTCA CAGCTGCTGT TGAGTTCATT AAAACCATTG ACGATCGCAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46319
    Clone ID Related Accession (Same CDS sequence) XM_018096657.1
    Accession Version XM_018096657.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4449bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG GTGAATGTTT GGGTTTAAGA
    ATAATTGACG TTCCAGAAGA GGAAAATCCG TGTTCAACAG GTCCTTCAAA TCGTTCCAAC
    TCAGTTTCCT CTTTGGATCT GGAAGGGGAG TCTGTATCTG AGCTAGGAGG AGGACCATCC
    GGAAGCAATG GGGTGGAGGC TTTGCAGTTA TTGGAACATG AACAAGCTAC CACACAGGAT
    AATCTAGATG ACAAACTGCG CAAATTTGAA ATTCGAGACA TGATGGGATT GACGGATGAT
    AGAGATATAT CAGAGACAGT CAGTGAGACA TGGAGCACGG ATGTGCTTGG AAGTGATTTT
    GACCCCAACA TTGATGAAGA TCGACTACAA GAAATTGCAG GTGCAGCAGC TGAGAACATG
    CTGGGAAGCC TGCTGTGCCT TCCCGGCTCT GGCTCAATAC TGCTTGATCC TTGTACTGGA
    TCCACTATAT CTGAGACTAC CAGTGAAGCA TGGAGTGTTG AGGTGCTCCC TAGTGATTCT
    GAAGCACCAG ATCTAAAGCA AGAGGAGCGG CTGCAGGAAC TGGAGAGCTG TTCAGGTTTG
    GGAAGCACAT CAGATGACAC TGAAGTTAGA GAGGTCAGCT CCCGGCCCAG CACTCCAGGA
    CTCAGTGTGG TGTCAGGTGA CTTTCCCAAA CCTTTCTATT CATTGCCGGG TATCAGTGCT
    ACCTCAGAGG ACATCCCAAA CAAGATAGAG GACCTACGCT CAGAGTGCAG CTCTGACTTT
    GGAGGAAAGG ATTCTGTTAC AAGTCCTGAG GCAGAAGAGT CTGTTCATGG ACCTCACCAT
    ATAACTGCAC CTCCAACCCA GACTGAATCC TTGTTGGCCA TGTTTGACCC ACTAGCTCCA
    GCATCCAATG AAGTGGTTCG ACCTAAAGTA CACTATGCGA GACCCTCACA TCCGCCTCCT
    GACCCGCCTG TACTGGAAGG AGCAAGTGGG GGAAATGAAG CTCGTCTTCC TATGTTCGGT
    TCTCATGTAT TTGTTCCCAT GGAGTCTGAA GCTTACAGGC AGAGACACTC ATGTCCTGAA
    AGACTGGTGC GCAGTCGCAG CTCCGACATC GCTTCTTCCA TACGGAGGCC AATGAGTGAC
    CCTGGCTGGG TGCGGCGTGG GGTGAATGAA GATAGGGAGC TGCCAACAGG AACTGCAATT
    AATGCACTAA TTAATCCCCC CCAGGCTTCA TCCTCCTCAC CAAGCAAGGA CTCTTCCAGG
    GGAGAGCCAG AAGAAAAAAA AGACAGCGAT GATGAGCGAT CAGATAGGAA TAAGCCTTGG
    TGGAAGAAGC GGTTTGTGTC TGCAATGCCC AAAGCTCCTA TCCCATTTAG GAAGAAGGAA
    AAACACGAGA AGGAGAGGGA AGACTTGGTA CAAGACAGGT TTTCAGTAGC AGATGACTCA
    ACATCTCAGC TAGGGCCACA TTCCCAGGCA GCAGAAGACA TCCTGGCTAA GTACAGGAAT
    GCTATTAAGA GGACAATCCC CAATGAAGGT TCCTCCGTGC CCTATGAAGG AGCAGATGCT
    GTTGGAGATG GAGAAAGCCT TCATGATTCT CCTCGTGATG AAGCTTTGCA GAACATGGCC
    TCAGATGACC TGCTTGATTC TGCCAGCCAA GTAGCCCACC CACAGGAGCC TGCATTCTCC
    TATAGGGATG CTAAGAAAAA ATTACGCCTG GCCCTGTGCT CTGCGGATTC AGTAGCGTTC
    CCTGTATTGT CTCATTCGAC ACGGAATGGC CTGCCAGATC ACACAGACCC AGAAGACAAT
    GAGATTGTGT GCTTCCTTAA AGTCCAACTT GCAGAGGCCA TAAACCTGCA GGACAAAAAC
    CTGATGGCTC AGATCCAGGA AACTATGCGA TGTGTGAGCC GATTTGACAG CCGCTCCTGC
    AGCAAAATTT TGGCATCCAT TGCAGAGGAT TACCGGAAGA GGGCCTCATA TATTGCATAT
    CTAACAAGAT GCCGACAGGG ACTACAGACC ACCCAGGCTC ACCTTGAGCG CCTCCTGCAG
    AGAGTGCTAC GAGATAAAGA GGTCTCCACG CGCTACTTCA CCACTGTCTG TGTGAGGCTT
    CTACTTGAGA GCAAAGAGAA GGAAATCCAT GAATTTATCC AAGATTTTCA AAAGTTAACT
    GCAGCTGATG ATAAAACAGC ACAAGTTGAA GAGTTCTTGC AATCACTTTA CGGGGCTATG
    GCACAAGATG TGATTTGGCA AAATGCCAGT GAGGAGCAGC TGCAAGATGC ACAGATTGCT
    ATTGAGCGAA GCATTATGAA CCGCATCTTT AAGCTGGCCT TCTATCCCAA TCAAGATGCA
    GATATTCTGC GAGATCAGGT CCTGCATGAA CACATTAAAA GGTTGTCTAA AGTGGTGACT
    GCCAATCACA GGGCACTGCA GATACCTGAG GTGTATCTGA GGGAGGCTCC TTGGCCATCT
    GCCCAAACCG AAATCCGTAC CATCAGCGCA TACAAGACAC CCAGGGACAA AGTGCAGTGC
    ATATTGCGCA TGTGCTCAAC AATCATGAAC CTTCTAAGCC TGGCCAATGA GTACTCTGTC
    CCAGGAGCAG ATGATTTTGT TCCTGTGCTG GTTTTTGTCC TTATTAAGGC TAATCCCCCC
    TGCCTACTAT CAACAGTGCA ATATATCAGC AACTTCTATT CCAATCGGCT CACTGGCGAG
    GAGTCCTACT GGTGGATGCA GTTCACAGCT GCTGTTGAGT TCATTAAAAC CATTGACGAT
    CGCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952146.1
    CDS169..4617
    Translation

    Target ORF information:

    RefSeq Version XM_018096657.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096657.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG GTGAATGTTT GGGTTTAAGA
    ATAATTGACG TTCCAGAAGA GGAAAATCCG TGTTCAACAG GTCCTTCAAA TCGTTCCAAC
    TCAGTTTCCT CTTTGGATCT GGAAGGGGAG TCTGTATCTG AGCTAGGAGG AGGACCATCC
    GGAAGCAATG GGGTGGAGGC TTTGCAGTTA TTGGAACATG AACAAGCTAC CACACAGGAT
    AATCTAGATG ACAAACTGCG CAAATTTGAA ATTCGAGACA TGATGGGATT GACGGATGAT
    AGAGATATAT CAGAGACAGT CAGTGAGACA TGGAGCACGG ATGTGCTTGG AAGTGATTTT
    GACCCCAACA TTGATGAAGA TCGACTACAA GAAATTGCAG GTGCAGCAGC TGAGAACATG
    CTGGGAAGCC TGCTGTGCCT TCCCGGCTCT GGCTCAATAC TGCTTGATCC TTGTACTGGA
    TCCACTATAT CTGAGACTAC CAGTGAAGCA TGGAGTGTTG AGGTGCTCCC TAGTGATTCT
    GAAGCACCAG ATCTAAAGCA AGAGGAGCGG CTGCAGGAAC TGGAGAGCTG TTCAGGTTTG
    GGAAGCACAT CAGATGACAC TGAAGTTAGA GAGGTCAGCT CCCGGCCCAG CACTCCAGGA
    CTCAGTGTGG TGTCAGGTGA CTTTCCCAAA CCTTTCTATT CATTGCCGGG TATCAGTGCT
    ACCTCAGAGG ACATCCCAAA CAAGATAGAG GACCTACGCT CAGAGTGCAG CTCTGACTTT
    GGAGGAAAGG ATTCTGTTAC AAGTCCTGAG GCAGAAGAGT CTGTTCATGG ACCTCACCAT
    ATAACTGCAC CTCCAACCCA GACTGAATCC TTGTTGGCCA TGTTTGACCC ACTAGCTCCA
    GCATCCAATG AAGTGGTTCG ACCTAAAGTA CACTATGCGA GACCCTCACA TCCGCCTCCT
    GACCCGCCTG TACTGGAAGG AGCAAGTGGG GGAAATGAAG CTCGTCTTCC TATGTTCGGT
    TCTCATGTAT TTGTTCCCAT GGAGTCTGAA GCTTACAGGC AGAGACACTC ATGTCCTGAA
    AGACTGGTGC GCAGTCGCAG CTCCGACATC GCTTCTTCCA TACGGAGGCC AATGAGTGAC
    CCTGGCTGGG TGCGGCGTGG GGTGAATGAA GATAGGGAGC TGCCAACAGG AACTGCAATT
    AATGCACTAA TTAATCCCCC CCAGGCTTCA TCCTCCTCAC CAAGCAAGGA CTCTTCCAGG
    GGAGAGCCAG AAGAAAAAAA AGACAGCGAT GATGAGCGAT CAGATAGGAA TAAGCCTTGG
    TGGAAGAAGC GGTTTGTGTC TGCAATGCCC AAAGCTCCTA TCCCATTTAG GAAGAAGGAA
    AAACACGAGA AGGAGAGGGA AGACTTGGTA CAAGACAGGT TTTCAGTAGC AGATGACTCA
    ACATCTCAGC TAGGGCCACA TTCCCAGGCA GCAGAAGACA TCCTGGCTAA GTACAGGAAT
    GCTATTAAGA GGACAATCCC CAATGAAGGT TCCTCCGTGC CCTATGAAGG AGCAGATGCT
    GTTGGAGATG GAGAAAGCCT TCATGATTCT CCTCGTGATG AAGCTTTGCA GAACATGGCC
    TCAGATGACC TGCTTGATTC TGCCAGCCAA GTAGCCCACC CACAGGAGCC TGCATTCTCC
    TATAGGGATG CTAAGAAAAA ATTACGCCTG GCCCTGTGCT CTGCGGATTC AGTAGCGTTC
    CCTGTATTGT CTCATTCGAC ACGGAATGGC CTGCCAGATC ACACAGACCC AGAAGACAAT
    GAGATTGTGT GCTTCCTTAA AGTCCAACTT GCAGAGGCCA TAAACCTGCA GGACAAAAAC
    CTGATGGCTC AGATCCAGGA AACTATGCGA TGTGTGAGCC GATTTGACAG CCGCTCCTGC
    AGCAAAATTT TGGCATCCAT TGCAGAGGAT TACCGGAAGA GGGCCTCATA TATTGCATAT
    CTAACAAGAT GCCGACAGGG ACTACAGACC ACCCAGGCTC ACCTTGAGCG CCTCCTGCAG
    AGAGTGCTAC GAGATAAAGA GGTCTCCACG CGCTACTTCA CCACTGTCTG TGTGAGGCTT
    CTACTTGAGA GCAAAGAGAA GGAAATCCAT GAATTTATCC AAGATTTTCA AAAGTTAACT
    GCAGCTGATG ATAAAACAGC ACAAGTTGAA GAGTTCTTGC AATCACTTTA CGGGGCTATG
    GCACAAGATG TGATTTGGCA AAATGCCAGT GAGGAGCAGC TGCAAGATGC ACAGATTGCT
    ATTGAGCGAA GCATTATGAA CCGCATCTTT AAGCTGGCCT TCTATCCCAA TCAAGATGCA
    GATATTCTGC GAGATCAGGT CCTGCATGAA CACATTAAAA GGTTGTCTAA AGTGGTGACT
    GCCAATCACA GGGCACTGCA GATACCTGAG GTGTATCTGA GGGAGGCTCC TTGGCCATCT
    GCCCAAACCG AAATCCGTAC CATCAGCGCA TACAAGACAC CCAGGGACAA AGTGCAGTGC
    ATATTGCGCA TGTGCTCAAC AATCATGAAC CTTCTAAGCC TGGCCAATGA GTACTCTGTC
    CCAGGAGCAG ATGATTTTGT TCCTGTGCTG GTTTTTGTCC TTATTAAGGC TAATCCCCCC
    TGCCTACTAT CAACAGTGCA ATATATCAGC AACTTCTATT CCAATCGGCT CACTGGCGAG
    GAGTCCTACT GGTGGATGCA GTTCACAGCT GCTGTTGAGT TCATTAAAAC CATTGACGAT
    CGCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46320
    Clone ID Related Accession (Same CDS sequence) XM_018096658.1
    Accession Version XM_018096658.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGAAG AGGAAAATCC GTGTTCAACA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGAAGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTG ACTTTCCCAA ACCTTTCTAT
    TCATTGCCGG GTATCAGTGC TACCTCAGAG GACATCCCAA ACAAGATAGA GGACCTACGC
    TCAGAGTGCA GCTCTGACTT TGGAGGAAAG GATTCTGTTA CAAGTCCTGA GGCAGAAGAG
    TCTGTTCATG TGGTTCGACC TAAAGTACAC TATGCGAGAC CCTCACATCC GCCTCCTGAC
    CCGCCTGTAC TGGAAGGAGC AAGTGGGGGA AATGAAGCTC GTCTTCCTAT GTTCGGTTCT
    CATGTATTTG TTCCCATGGA GTCTGAAGCT TACAGGCAGA GACACTCATG TCCTGAAAGA
    CTGGTGCGCA GTCGCAGCTC CGACATCGCT TCTTCCATAC GGAGGCCAAT GAGTGACCCT
    GGCTGGGTGC GGCGTGGGGT GAATGAAGAT AGGGAGCTGC CAACAGGAAC TGCAATTAAT
    GCACTAATTA ATCCCCCCCA GGCTTCATCC TCCTCACCAA GCAAGGACTC TTCCAGGGGA
    GAGCCAGAAG AAAAAAAAGA CAGCGATGAT GAGCGATCAG ATAGGAATAA GCCTTGGTGG
    AAGAAGCGGT TTGTGTCTGC AATGCCCAAA GCTCCTATCC CATTTAGGAA GAAGGAAAAA
    CACGAGAAGG AGAGGGAAGA CTTGGTACAA GACAGGTTTT CAGTAGCAGA TGACTCAACA
    TCTCAGCTAG GGCCACATTC CCAGGCAGCA GAAGACATCC TGGCTAAGTA CAGGAATGCT
    ATTAAGAGGA CAATCCCCAA TGAAGGTTCC TCCGTGCCCT ATGAAGGAGC AGATGCTGTT
    GGAGATGGAG AAAGCCTTCA TGATTCTCCT CGTGATGAAG CTTTGCAGAA CATGGCCTCA
    GATGACCTGC TTGATTCTGC CAGCCAAGTA GCCCACCCAC AGGAGCCTGC ATTCTCCTAT
    AGGGATGCTA AGAAAAAATT ACGCCTGGCC CTGTGCTCTG CGGATTCAGT AGCGTTCCCT
    GTATTGTCTC ATTCGACACG GAATGGCCTG CCAGATCACA CAGACCCAGA AGACAATGAG
    ATTGTGTGCT TCCTTAAAGT CCAACTTGCA GAGGCCATAA ACCTGCAGGA CAAAAACCTG
    ATGGCTCAGA TCCAGGAAAC TATGCGATGT GTGAGCCGAT TTGACAGCCG CTCCTGCAGC
    AAAATTTTGG CATCCATTGC AGAGGATTAC CGGAAGAGGG CCTCATATAT TGCATATCTA
    ACAAGATGCC GACAGGGACT ACAGACCACC CAGGCTCACC TTGAGCGCCT CCTGCAGAGA
    GTGCTACGAG ATAAAGAGGT CTCCACGCGC TACTTCACCA CTGTCTGTGT GAGGCTTCTA
    CTTGAGAGCA AAGAGAAGGA AATCCATGAA TTTATCCAAG ATTTTCAAAA GTTAACTGCA
    GCTGATGATA AAACAGCACA AGTTGAAGAG TTCTTGCAAT CACTTTACGG GGCTATGGCA
    CAAGATGTGA TTTGGCAAAA TGCCAGTGAG GAGCAGCTGC AAGATGCACA GATTGCTATT
    GAGCGAAGCA TTATGAACCG CATCTTTAAG CTGGCCTTCT ATCCCAATCA AGATGCAGAT
    ATTCTGCGAG ATCAGGTCCT GCATGAACAC ATTAAAAGGT TGTCTAAAGT GGTGACTGCC
    AATCACAGGG CACTGCAGAT ACCTGAGGTG TATCTGAGGG AGGCTCCTTG GCCATCTGCC
    CAAACCGAAA TCCGTACCAT CAGCGCATAC AAGACACCCA GGGACAAAGT GCAGTGCATA
    TTGCGCATGT GCTCAACAAT CATGAACCTT CTAAGCCTGG CCAATGAGTA CTCTGTCCCA
    GGAGCAGATG ATTTTGTTCC TGTGCTGGTT TTTGTCCTTA TTAAGGCTAA TCCCCCCTGC
    CTACTATCAA CAGTGCAATA TATCAGCAAC TTCTATTCCA ATCGGCTCAC TGGCGAGGAG
    TCCTACTGGT GGATGCAGTT CACAGCTGCT GTTGAGTTCA TTAAAACCAT TGACGATCGC
    AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952147.1
    CDS172..4617
    Translation

    Target ORF information:

    RefSeq Version XM_018096658.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096658.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGAAG AGGAAAATCC GTGTTCAACA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGAAGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTG ACTTTCCCAA ACCTTTCTAT
    TCATTGCCGG GTATCAGTGC TACCTCAGAG GACATCCCAA ACAAGATAGA GGACCTACGC
    TCAGAGTGCA GCTCTGACTT TGGAGGAAAG GATTCTGTTA CAAGTCCTGA GGCAGAAGAG
    TCTGTTCATG TGGTTCGACC TAAAGTACAC TATGCGAGAC CCTCACATCC GCCTCCTGAC
    CCGCCTGTAC TGGAAGGAGC AAGTGGGGGA AATGAAGCTC GTCTTCCTAT GTTCGGTTCT
    CATGTATTTG TTCCCATGGA GTCTGAAGCT TACAGGCAGA GACACTCATG TCCTGAAAGA
    CTGGTGCGCA GTCGCAGCTC CGACATCGCT TCTTCCATAC GGAGGCCAAT GAGTGACCCT
    GGCTGGGTGC GGCGTGGGGT GAATGAAGAT AGGGAGCTGC CAACAGGAAC TGCAATTAAT
    GCACTAATTA ATCCCCCCCA GGCTTCATCC TCCTCACCAA GCAAGGACTC TTCCAGGGGA
    GAGCCAGAAG AAAAAAAAGA CAGCGATGAT GAGCGATCAG ATAGGAATAA GCCTTGGTGG
    AAGAAGCGGT TTGTGTCTGC AATGCCCAAA GCTCCTATCC CATTTAGGAA GAAGGAAAAA
    CACGAGAAGG AGAGGGAAGA CTTGGTACAA GACAGGTTTT CAGTAGCAGA TGACTCAACA
    TCTCAGCTAG GGCCACATTC CCAGGCAGCA GAAGACATCC TGGCTAAGTA CAGGAATGCT
    ATTAAGAGGA CAATCCCCAA TGAAGGTTCC TCCGTGCCCT ATGAAGGAGC AGATGCTGTT
    GGAGATGGAG AAAGCCTTCA TGATTCTCCT CGTGATGAAG CTTTGCAGAA CATGGCCTCA
    GATGACCTGC TTGATTCTGC CAGCCAAGTA GCCCACCCAC AGGAGCCTGC ATTCTCCTAT
    AGGGATGCTA AGAAAAAATT ACGCCTGGCC CTGTGCTCTG CGGATTCAGT AGCGTTCCCT
    GTATTGTCTC ATTCGACACG GAATGGCCTG CCAGATCACA CAGACCCAGA AGACAATGAG
    ATTGTGTGCT TCCTTAAAGT CCAACTTGCA GAGGCCATAA ACCTGCAGGA CAAAAACCTG
    ATGGCTCAGA TCCAGGAAAC TATGCGATGT GTGAGCCGAT TTGACAGCCG CTCCTGCAGC
    AAAATTTTGG CATCCATTGC AGAGGATTAC CGGAAGAGGG CCTCATATAT TGCATATCTA
    ACAAGATGCC GACAGGGACT ACAGACCACC CAGGCTCACC TTGAGCGCCT CCTGCAGAGA
    GTGCTACGAG ATAAAGAGGT CTCCACGCGC TACTTCACCA CTGTCTGTGT GAGGCTTCTA
    CTTGAGAGCA AAGAGAAGGA AATCCATGAA TTTATCCAAG ATTTTCAAAA GTTAACTGCA
    GCTGATGATA AAACAGCACA AGTTGAAGAG TTCTTGCAAT CACTTTACGG GGCTATGGCA
    CAAGATGTGA TTTGGCAAAA TGCCAGTGAG GAGCAGCTGC AAGATGCACA GATTGCTATT
    GAGCGAAGCA TTATGAACCG CATCTTTAAG CTGGCCTTCT ATCCCAATCA AGATGCAGAT
    ATTCTGCGAG ATCAGGTCCT GCATGAACAC ATTAAAAGGT TGTCTAAAGT GGTGACTGCC
    AATCACAGGG CACTGCAGAT ACCTGAGGTG TATCTGAGGG AGGCTCCTTG GCCATCTGCC
    CAAACCGAAA TCCGTACCAT CAGCGCATAC AAGACACCCA GGGACAAAGT GCAGTGCATA
    TTGCGCATGT GCTCAACAAT CATGAACCTT CTAAGCCTGG CCAATGAGTA CTCTGTCCCA
    GGAGCAGATG ATTTTGTTCC TGTGCTGGTT TTTGTCCTTA TTAAGGCTAA TCCCCCCTGC
    CTACTATCAA CAGTGCAATA TATCAGCAAC TTCTATTCCA ATCGGCTCAC TGGCGAGGAG
    TCCTACTGGT GGATGCAGTT CACAGCTGCT GTTGAGTTCA TTAAAACCAT TGACGATCGC
    AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa64952
    Clone ID Related Accession (Same CDS sequence) XM_031891626.1
    Accession Version XM_031891626.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGGTC CTTCAAATCG TTCCAACTCA
    GTTTCCTCTT TGGATCTGGA AGGGGAGTCT GTATCTGAGC TAGGAGGAGG ACCATCCGGA
    AGCAATGGGG TGGAGGCTTT GCAGTTATTG GAACATGAAC AAGCTACCAC ACAGGATAAT
    CTAGATGACA AACTGCGCAA ATTTGAAATT CGAGACATGA TGGGATTGAC GGATGATAGA
    GATATATCAG AGACAGTCAG TGAGACATGG AGCACGGATG TGCTTGGAAG TGATTTTGAC
    CCCAACATTG ATGAAGATCG ACTACAAGAA ATTGCAGGTG CAGCAGCTGA GAACATGCTG
    GGAAGCCTGC TGTGCCTTCC CGGCTCTGGC TCAATACTGC TTGATCCTTG TACTGGATCC
    ACTATATCTG AGACTACCAG TGAAGCATGG AGTGTTGAGG TGCTCCCTAG TGATTCTGAA
    GCACCAGATC TAAAGCAAGA GGAGCGGCTG CAGGAACTGG AGAGCTGTTC AGGTTTGGGA
    AGCACATCAG ATGACACTGA AGTTAGAGAG GTCAGCTCCC GGCCCAGCAC TCCAGGACTC
    AGTGTGGTGT CAGGTGACTT TCCCAAACCT TTCTATTCAT TGCCGGGTAT CAGTGCTACC
    TCAGAGGACA TCCCAAACAA GATAGAGGAC CTACGCTCAG AGTGCAGCTC TGACTTTGGA
    GGAAAGGATT CTGTTACAAG TCCTGAGGCA GAAGAGTCTG TTCATGTGGT TCGACCTAAA
    GTACACTATG CGAGACCCTC ACATCCGCCT CCTGACCCGC CTGTACTGGA AGGAGCAAGT
    GGGGGAAATG AAGCTCGTCT TCCTATGTTC GGTTCTCATG TATTTGTTCC CATGGAGTCT
    GAAGCTTACA GGCAGAGACA CTCATGTCCT GAAAGACTGG TGCGCAGTCG CAGCTCCGAC
    ATCGCTTCTT CCATACGGAG GCCAATGAGT GACCCTGGCT GGGTGCGGCG TGGGGTGAAT
    GAAGATAGGG AGCTGCCAAC AGGAACTGCA ATTAATGCAC TAATTAATCC CCCCCAGGCT
    TCATCCTCCT CACCAAGCAA GGACTCTTCC AGGGGAGAGC CAGAAGAAAA AAAAGACAGC
    GATGATGAGC GATCAGATAG GAATAAGCCT TGGTGGAAGA AGCGGTTTGT GTCTGCAATG
    CCCAAAGCTC CTATCCCATT TAGGAAGAAG GAAAAACACG AGAAGGAGAG GGAAGACTTG
    GTACAAGACA GGTTTTCAGT AGCAGATGAC TCAACATCTC AGCTAGGGCC ACATTCCCAG
    GCAGCAGAAG ACATCCTGGC TAAGTACAGG AATGCTATTA AGAGGACAAT CCCCAATGAA
    GGTTCCTCCG TGCCCTATGA AGGAGCAGAT GCTGTTGGAG ATGGAGAAAG CCTTCATGAT
    TCTCCTCGTG ATGAAGCTTT GCAGAACATG GCCTCAGATG ACCTGCTTGA TTCTGCCAGC
    CAAGTAGCCC ACCCACAGGA GCCTGCATTC TCCTATAGGG ATGCTAAGAA AAAATTACGC
    CTGGCCCTGT GCTCTGCGGA TTCAGTAGCG TTCCCTGTAT TGTCTCATTC GACACGGAAT
    GGCCTGCCAG ATCACACAGA CCCAGAAGAC AATGAGATTG TGTGCTTCCT TAAAGTCCAA
    CTTGCAGAGG CCATAAACCT GCAGGACAAA AACCTGATGG CTCAGATCCA GGAAACTATG
    CGATGTGTGA GCCGATTTGA CAGCCGCTCC TGCAGCAAAA TTTTGGCATC CATTGCAGAG
    GATTACCGGA AGAGGGCCTC ATATATTGCA TATCTAACAA GATGCCGACA GGGACTACAG
    ACCACCCAGG CTCACCTTGA GCGCCTCCTG CAGAGAGTGC TACGAGATAA AGAGGTCTCC
    ACGCGCTACT TCACCACTGT CTGTGTGAGG CTTCTACTTG AGAGCAAAGA GAAGGAAATC
    CATGAATTTA TCCAAGATTT TCAAAAGTTA ACTGCAGCTG ATGATAAAAC AGCACAAGTT
    GAAGAGTTCT TGCAATCACT TTACGGGGCT ATGGCACAAG ATGTGATTTG GCAAAATGCC
    AGTGAGGAGC AGCTGCAAGA TGCACAGATT GCTATTGAGC GAAGCATTAT GAACCGCATC
    TTTAAGCTGG CCTTCTATCC CAATCAAGAT GCAGATATTC TGCGAGATCA GGTCCTGCAT
    GAACACATTA AAAGGTTGTC TAAAGTGGTG ACTGCCAATC ACAGGGCACT GCAGATACCT
    GAGGTGTATC TGAGGGAGGC TCCTTGGCCA TCTGCCCAAA CCGAAATCCG TACCATCAGC
    GCATACAAGA CACCCAGGGA CAAAGTGCAG TGCATATTGC GCATGTGCTC AACAATCATG
    AACCTTCTAA GCCTGGCCAA TGAGTACTCT GTCCCAGGAG CAGATGATTT TGTTCCTGTG
    CTGGTTTTTG TCCTTATTAA GGCTAATCCC CCCTGCCTAC TATCAACAGT GCAATATATC
    AGCAACTTCT ATTCCAATCG GCTCACTGGC GAGGAGTCCT ACTGGTGGAT GCAGTTCACA
    GCTGCTGTTG AGTTCATTAA AACCATTGAC GATCGCAAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031747486.1
    CDS162..4583
    Translation

    Target ORF information:

    RefSeq Version XM_031891626.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031891626.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGGTC CTTCAAATCG TTCCAACTCA
    GTTTCCTCTT TGGATCTGGA AGGGGAGTCT GTATCTGAGC TAGGAGGAGG ACCATCCGGA
    AGCAATGGGG TGGAGGCTTT GCAGTTATTG GAACATGAAC AAGCTACCAC ACAGGATAAT
    CTAGATGACA AACTGCGCAA ATTTGAAATT CGAGACATGA TGGGATTGAC GGATGATAGA
    GATATATCAG AGACAGTCAG TGAGACATGG AGCACGGATG TGCTTGGAAG TGATTTTGAC
    CCCAACATTG ATGAAGATCG ACTACAAGAA ATTGCAGGTG CAGCAGCTGA GAACATGCTG
    GGAAGCCTGC TGTGCCTTCC CGGCTCTGGC TCAATACTGC TTGATCCTTG TACTGGATCC
    ACTATATCTG AGACTACCAG TGAAGCATGG AGTGTTGAGG TGCTCCCTAG TGATTCTGAA
    GCACCAGATC TAAAGCAAGA GGAGCGGCTG CAGGAACTGG AGAGCTGTTC AGGTTTGGGA
    AGCACATCAG ATGACACTGA AGTTAGAGAG GTCAGCTCCC GGCCCAGCAC TCCAGGACTC
    AGTGTGGTGT CAGGTGACTT TCCCAAACCT TTCTATTCAT TGCCGGGTAT CAGTGCTACC
    TCAGAGGACA TCCCAAACAA GATAGAGGAC CTACGCTCAG AGTGCAGCTC TGACTTTGGA
    GGAAAGGATT CTGTTACAAG TCCTGAGGCA GAAGAGTCTG TTCATGTGGT TCGACCTAAA
    GTACACTATG CGAGACCCTC ACATCCGCCT CCTGACCCGC CTGTACTGGA AGGAGCAAGT
    GGGGGAAATG AAGCTCGTCT TCCTATGTTC GGTTCTCATG TATTTGTTCC CATGGAGTCT
    GAAGCTTACA GGCAGAGACA CTCATGTCCT GAAAGACTGG TGCGCAGTCG CAGCTCCGAC
    ATCGCTTCTT CCATACGGAG GCCAATGAGT GACCCTGGCT GGGTGCGGCG TGGGGTGAAT
    GAAGATAGGG AGCTGCCAAC AGGAACTGCA ATTAATGCAC TAATTAATCC CCCCCAGGCT
    TCATCCTCCT CACCAAGCAA GGACTCTTCC AGGGGAGAGC CAGAAGAAAA AAAAGACAGC
    GATGATGAGC GATCAGATAG GAATAAGCCT TGGTGGAAGA AGCGGTTTGT GTCTGCAATG
    CCCAAAGCTC CTATCCCATT TAGGAAGAAG GAAAAACACG AGAAGGAGAG GGAAGACTTG
    GTACAAGACA GGTTTTCAGT AGCAGATGAC TCAACATCTC AGCTAGGGCC ACATTCCCAG
    GCAGCAGAAG ACATCCTGGC TAAGTACAGG AATGCTATTA AGAGGACAAT CCCCAATGAA
    GGTTCCTCCG TGCCCTATGA AGGAGCAGAT GCTGTTGGAG ATGGAGAAAG CCTTCATGAT
    TCTCCTCGTG ATGAAGCTTT GCAGAACATG GCCTCAGATG ACCTGCTTGA TTCTGCCAGC
    CAAGTAGCCC ACCCACAGGA GCCTGCATTC TCCTATAGGG ATGCTAAGAA AAAATTACGC
    CTGGCCCTGT GCTCTGCGGA TTCAGTAGCG TTCCCTGTAT TGTCTCATTC GACACGGAAT
    GGCCTGCCAG ATCACACAGA CCCAGAAGAC AATGAGATTG TGTGCTTCCT TAAAGTCCAA
    CTTGCAGAGG CCATAAACCT GCAGGACAAA AACCTGATGG CTCAGATCCA GGAAACTATG
    CGATGTGTGA GCCGATTTGA CAGCCGCTCC TGCAGCAAAA TTTTGGCATC CATTGCAGAG
    GATTACCGGA AGAGGGCCTC ATATATTGCA TATCTAACAA GATGCCGACA GGGACTACAG
    ACCACCCAGG CTCACCTTGA GCGCCTCCTG CAGAGAGTGC TACGAGATAA AGAGGTCTCC
    ACGCGCTACT TCACCACTGT CTGTGTGAGG CTTCTACTTG AGAGCAAAGA GAAGGAAATC
    CATGAATTTA TCCAAGATTT TCAAAAGTTA ACTGCAGCTG ATGATAAAAC AGCACAAGTT
    GAAGAGTTCT TGCAATCACT TTACGGGGCT ATGGCACAAG ATGTGATTTG GCAAAATGCC
    AGTGAGGAGC AGCTGCAAGA TGCACAGATT GCTATTGAGC GAAGCATTAT GAACCGCATC
    TTTAAGCTGG CCTTCTATCC CAATCAAGAT GCAGATATTC TGCGAGATCA GGTCCTGCAT
    GAACACATTA AAAGGTTGTC TAAAGTGGTG ACTGCCAATC ACAGGGCACT GCAGATACCT
    GAGGTGTATC TGAGGGAGGC TCCTTGGCCA TCTGCCCAAA CCGAAATCCG TACCATCAGC
    GCATACAAGA CACCCAGGGA CAAAGTGCAG TGCATATTGC GCATGTGCTC AACAATCATG
    AACCTTCTAA GCCTGGCCAA TGAGTACTCT GTCCCAGGAG CAGATGATTT TGTTCCTGTG
    CTGGTTTTTG TCCTTATTAA GGCTAATCCC CCCTGCCTAC TATCAACAGT GCAATATATC
    AGCAACTTCT ATTCCAATCG GCTCACTGGC GAGGAGTCCT ACTGGTGGAT GCAGTTCACA
    GCTGCTGTTG AGTTCATTAA AACCATTGAC GATCGCAAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa64953
    Clone ID Related Accession (Same CDS sequence) XM_031891627.1
    Accession Version XM_031891627.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG GTCCTTCAAA TCGTTCCAAC
    TCAGTTTCCT CTTTGGATCT GGAAGGGGAG TCTGTATCTG AGCTAGGAGG AGGACCATCC
    GGAAGCAATG GGGTGGAGGC TTTGCAGTTA TTGGAACATG AACAAGCTAC CACACAGGAT
    AATCTAGATG ACAAACTGCG CAAATTTGAA ATTCGAGACA TGATGGGATT GACGGATGAT
    AGAGATATAT CAGAGACAGT CAGTGAGACA TGGAGCACGG ATGTGCTTGG AAGTGATTTT
    GACCCCAACA TTGATGAAGA TCGACTACAA GAAATTGCAG GTGCAGCAGC TGAGAACATG
    CTGGGAAGCC TGCTGTGCCT TCCCGGCTCT GGCTCAATAC TGCTTGATCC TTGTACTGGA
    TCCACTATAT CTGAGACTAC CAGTGAAGCA TGGAGTGTTG AGGTGCTCCC TAGTGATTCT
    GAAGCACCAG ATCTAAAGCA AGAGGAGCGG CTGCAGGAAC TGGAGAGCTG TTCAGGTTTG
    GGAAGCACAT CAGATGACAC TGAAGTTAGA GAGGTCAGCT CCCGGCCCAG CACTCCAGGA
    CTCAGTGTGG TGTCAGGTGA CTTTCCCAAA CCTTTCTATT CATTGCCGGG TATCAGTGCT
    ACCTCAGAGG ACATCCCAAA CAAGATAGAG GACCTACGCT CAGAGTGCAG CTCTGACTTT
    GGAGGAAAGG ATTCTGTTAC AAGTCCTGAG GCAGAAGAGT CTGTTCATGG ACCTCACCAT
    ATAACTGCAC CTCCAACCCA GACTGAATCC TTGTTGGCCA TGTTTGACCC ACTAGCTCCA
    GCATCCAATG AAGTGGTTCG ACCTAAAGTA CACTATGCGA GACCCTCACA TCCGCCTCCT
    GACCCGCCTG TACTGGAAGG AGCAAGTGGG GGAAATGAAG CTCGTCTTCC TATGTTCGGT
    TCTCATGTAT TTGTTCCCAT GGAGTCTGAA GCTTACAGGC AGAGACACTC ATGTCCTGAA
    AGACTGGTGC GCAGTCGCAG CTCCGACATC GCTTCTTCCA TACGGAGGCC AATGAGTGAC
    CCTGGCTGGG TGCGGCGTGG GGTGAATGAA GATAGGGAGC TGCCAACAGG AACTGCAATT
    AATGCACTAA TTAATCCCCC CCAGGCTTCA TCCTCCTCAC CAAGCAAGGA CTCTTCCAGG
    GGAGAGCCAG AAGAAAAAAA AGACAGCGAT GATGAGCGAT CAGATAGGAA TAAGCCTTGG
    TGGAAGAAGC GGTTTGTGTC TGCAATGCCC AAAGCTCCTA TCCCATTTAG GAAGAAGGAA
    AAACACGAGA AGGAGAGGGA AGACTTGGTA CAAGACAGGT TTTCAGTAGC AGATGACTCA
    ACATCTCAGC TAGGGCCACA TTCCCAGGCA GCAGAAGACA TCCTGGCTAA GTACAGGAAT
    GCTATTAAGA GGACAATCCC CAATGAAGGT TCCTCCGTGC CCTATGAAGG AGCAGATGCT
    GTTGGAGATG GAGAAAGCCT TCATGATTCT CCTCGTGATG AAGCTTTGCA GAACATGGCC
    TCAGATGACC TGCTTGATTC TGCCAGCCAA GTAGCCCACC CACAGGAGCC TGCATTCTCC
    TATAGGGATG CTAAGAAAAA ATTACGCCTG GCCCTGTGCT CTGCGGATTC AGTAGCGTTC
    CCTGTATTGT CTCATTCGAC ACGGAATGGC CTGCCAGATC ACACAGACCC AGAAGACAAT
    GAGATTGTGT GCTTCCTTAA AGTCCAACTT GCAGAGGCCA TAAACCTGCA GGACAAAAAC
    CTGATGGCTC AGATCCAGGA AACTATGCGA TGTGTGAGCC GATTTGACAG CCGCTCCTGC
    AGCAAAATTT TGGCATCCAT TGCAGAGGAT TACCGGAAGA GGGCCTCATA TATTGCATAT
    CTAACAAGAT GCCGACAGGG ACTACAGACC ACCCAGGCTC ACCTTGAGCG CCTCCTGCAG
    AGAGTGCTAC GAGATAAAGA GGTCTCCACG CGCTACTTCA CCACTGTCTG TGTGAGGCTT
    CTACTTGAGA GCAAAGAGAA GGAAATCCAT GAATTTATCC AAGATTTTCA AAAGTTAACT
    GCAGCTGATG ATAAAACAGC ACAAGTTGAA GAGTTCTTGC AATCACTTTA CGGGGCTATG
    GCACAAGATG TGATTTGGCA AAATGCCAGT GAGGAGCAGC TGCAAGATGC ACAGATTGCT
    ATTGAGCGAA GCATTATGAA CCGCATCTTT AAGCTGGCCT TCTATCCCAA TCAAGATGCA
    GATATTCTGC GAGATCAGGT CCTGCATGAA CACATTAAAA GGTTGTCTAA AGTGGTGACT
    GCCAATCACA GGGCACTGCA GATACCTGAG GTGTATCTGA GGGAGGCTCC TTGGCCATCT
    GCCCAAACCG AAATCCGTAC CATCAGCGCA TACAAGACAC CCAGGGACAA AGTGCAGTGC
    ATATTGCGCA TGTGCTCAAC AATCATGAAC CTTCTAAGCC TGGCCAATGA GTACTCTGTC
    CCAGGAGCAG ATGATTTTGT TCCTGTGCTG GTTTTTGTCC TTATTAAGGC TAATCCCCCC
    TGCCTACTAT CAACAGTGCA ATATATCAGC AACTTCTATT CCAATCGGCT CACTGGCGAG
    GAGTCCTACT GGTGGATGCA GTTCACAGCT GCTGTTGAGT TCATTAAAAC CATTGACGAT
    CGCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031747487.1
    CDS159..4547
    Translation

    Target ORF information:

    RefSeq Version XM_031891627.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031891627.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG GTCCTTCAAA TCGTTCCAAC
    TCAGTTTCCT CTTTGGATCT GGAAGGGGAG TCTGTATCTG AGCTAGGAGG AGGACCATCC
    GGAAGCAATG GGGTGGAGGC TTTGCAGTTA TTGGAACATG AACAAGCTAC CACACAGGAT
    AATCTAGATG ACAAACTGCG CAAATTTGAA ATTCGAGACA TGATGGGATT GACGGATGAT
    AGAGATATAT CAGAGACAGT CAGTGAGACA TGGAGCACGG ATGTGCTTGG AAGTGATTTT
    GACCCCAACA TTGATGAAGA TCGACTACAA GAAATTGCAG GTGCAGCAGC TGAGAACATG
    CTGGGAAGCC TGCTGTGCCT TCCCGGCTCT GGCTCAATAC TGCTTGATCC TTGTACTGGA
    TCCACTATAT CTGAGACTAC CAGTGAAGCA TGGAGTGTTG AGGTGCTCCC TAGTGATTCT
    GAAGCACCAG ATCTAAAGCA AGAGGAGCGG CTGCAGGAAC TGGAGAGCTG TTCAGGTTTG
    GGAAGCACAT CAGATGACAC TGAAGTTAGA GAGGTCAGCT CCCGGCCCAG CACTCCAGGA
    CTCAGTGTGG TGTCAGGTGA CTTTCCCAAA CCTTTCTATT CATTGCCGGG TATCAGTGCT
    ACCTCAGAGG ACATCCCAAA CAAGATAGAG GACCTACGCT CAGAGTGCAG CTCTGACTTT
    GGAGGAAAGG ATTCTGTTAC AAGTCCTGAG GCAGAAGAGT CTGTTCATGG ACCTCACCAT
    ATAACTGCAC CTCCAACCCA GACTGAATCC TTGTTGGCCA TGTTTGACCC ACTAGCTCCA
    GCATCCAATG AAGTGGTTCG ACCTAAAGTA CACTATGCGA GACCCTCACA TCCGCCTCCT
    GACCCGCCTG TACTGGAAGG AGCAAGTGGG GGAAATGAAG CTCGTCTTCC TATGTTCGGT
    TCTCATGTAT TTGTTCCCAT GGAGTCTGAA GCTTACAGGC AGAGACACTC ATGTCCTGAA
    AGACTGGTGC GCAGTCGCAG CTCCGACATC GCTTCTTCCA TACGGAGGCC AATGAGTGAC
    CCTGGCTGGG TGCGGCGTGG GGTGAATGAA GATAGGGAGC TGCCAACAGG AACTGCAATT
    AATGCACTAA TTAATCCCCC CCAGGCTTCA TCCTCCTCAC CAAGCAAGGA CTCTTCCAGG
    GGAGAGCCAG AAGAAAAAAA AGACAGCGAT GATGAGCGAT CAGATAGGAA TAAGCCTTGG
    TGGAAGAAGC GGTTTGTGTC TGCAATGCCC AAAGCTCCTA TCCCATTTAG GAAGAAGGAA
    AAACACGAGA AGGAGAGGGA AGACTTGGTA CAAGACAGGT TTTCAGTAGC AGATGACTCA
    ACATCTCAGC TAGGGCCACA TTCCCAGGCA GCAGAAGACA TCCTGGCTAA GTACAGGAAT
    GCTATTAAGA GGACAATCCC CAATGAAGGT TCCTCCGTGC CCTATGAAGG AGCAGATGCT
    GTTGGAGATG GAGAAAGCCT TCATGATTCT CCTCGTGATG AAGCTTTGCA GAACATGGCC
    TCAGATGACC TGCTTGATTC TGCCAGCCAA GTAGCCCACC CACAGGAGCC TGCATTCTCC
    TATAGGGATG CTAAGAAAAA ATTACGCCTG GCCCTGTGCT CTGCGGATTC AGTAGCGTTC
    CCTGTATTGT CTCATTCGAC ACGGAATGGC CTGCCAGATC ACACAGACCC AGAAGACAAT
    GAGATTGTGT GCTTCCTTAA AGTCCAACTT GCAGAGGCCA TAAACCTGCA GGACAAAAAC
    CTGATGGCTC AGATCCAGGA AACTATGCGA TGTGTGAGCC GATTTGACAG CCGCTCCTGC
    AGCAAAATTT TGGCATCCAT TGCAGAGGAT TACCGGAAGA GGGCCTCATA TATTGCATAT
    CTAACAAGAT GCCGACAGGG ACTACAGACC ACCCAGGCTC ACCTTGAGCG CCTCCTGCAG
    AGAGTGCTAC GAGATAAAGA GGTCTCCACG CGCTACTTCA CCACTGTCTG TGTGAGGCTT
    CTACTTGAGA GCAAAGAGAA GGAAATCCAT GAATTTATCC AAGATTTTCA AAAGTTAACT
    GCAGCTGATG ATAAAACAGC ACAAGTTGAA GAGTTCTTGC AATCACTTTA CGGGGCTATG
    GCACAAGATG TGATTTGGCA AAATGCCAGT GAGGAGCAGC TGCAAGATGC ACAGATTGCT
    ATTGAGCGAA GCATTATGAA CCGCATCTTT AAGCTGGCCT TCTATCCCAA TCAAGATGCA
    GATATTCTGC GAGATCAGGT CCTGCATGAA CACATTAAAA GGTTGTCTAA AGTGGTGACT
    GCCAATCACA GGGCACTGCA GATACCTGAG GTGTATCTGA GGGAGGCTCC TTGGCCATCT
    GCCCAAACCG AAATCCGTAC CATCAGCGCA TACAAGACAC CCAGGGACAA AGTGCAGTGC
    ATATTGCGCA TGTGCTCAAC AATCATGAAC CTTCTAAGCC TGGCCAATGA GTACTCTGTC
    CCAGGAGCAG ATGATTTTGT TCCTGTGCTG GTTTTTGTCC TTATTAAGGC TAATCCCCCC
    TGCCTACTAT CAACAGTGCA ATATATCAGC AACTTCTATT CCAATCGGCT CACTGGCGAG
    GAGTCCTACT GGTGGATGCA GTTCACAGCT GCTGTTGAGT TCATTAAAAC CATTGACGAT
    CGCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14180
    Clone ID Related Accession (Same CDS sequence) XM_004916738.3 , XM_004916738.4
    Accession Version XM_004916738.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916738.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGGTC CTTCAAATCG TTCCAACTCA
    GTTTCCTCTT TGGATCTGGA AGGGGAGTCT GTATCTGAGC TAGGAGGAGG ACCATCCGGA
    AGCAATGGGG TGGAGGCTTT GCAGTTATTG GAACATGAAC AAGCTACCAC ACAGGATAAT
    CTAGATGACA AACTGCGCAA ATTTGAAATT CGAGACATGA TGGGATTGAC GGATGATAGA
    GATATATCAG AGACAGTCAG TGAGACATGG AGCACGGATG TGCTTGGAAG TGATTTTGAC
    CCCAACATTG ATGAAGATCG ACTACAAGAA ATTGCAGGTG CAGCAGCTGA GAACATGCTG
    GGAAGCCTGC TGTGCCTTCC CGGCTCTGGC TCAATACTGC TTGATCCTTG TACTGGATCC
    ACTATATCTG AGACTACCAG TGAAGCATGG AGTGTTGAGG TGCTCCCTAG TGATTCTGAA
    GCACCAGATC TAAAGCAAGA GGAGCGGCTG CAGGAACTGG AGAGCTGTTC AGGTTTGGGA
    AGCACATCAG ATGACACTGA AGTTAGAGAG GTCAGCTCCC GGCCCAGCAC TCCAGGACTC
    AGTGTGGTGT CAGGTGACTT TCCCAAACCT TTCTATTCAT TGCCGGGTAT CAGTGCTACC
    TCAGAGGACA TCCCAAACAA GATAGAGGAC CTACGCTCAG AGTGCAGCTC TGACTTTGGA
    GGAAAGGATT CTGTTACAAG TCCTGAGGCA GAAGAGTCTG TTCATGGACC TCACCATATA
    ACTGCACCTC CAACCCAGAC TGAATCCTTG TTGGCCATGT TTGACCCACT AGCTCCAGCA
    TCCAATGAAG TGGTTCGACC TAAAGTACAC TATGCGAGAC CCTCACATCC GCCTCCTGAC
    CCGCCTGTAC TGGAAGGAGC AAGTGGGGGA AATGAAGCTC GTCTTCCTAT GTTCGGTTCT
    CATGTATTTG TTCCCATGGA GTCTGAAGCT TACAGGCAGA GACACTCATG TCCTGAAAGA
    CTGGTGCGCA GTCGCAGCTC CGACATCGCT TCTTCCATAC GGAGGCCAAT GAGTGACCCT
    GGCTGGGTGC GGCGTGGGGT GAATGAAGAT AGGGAGCTGC CAACAGGAAC TGCAATTAAT
    GCACTAATTA ATCCCCCCCA GGCTTCATCC TCCTCACCAA GCAAGGACTC TTCCAGGGGA
    GAGCCAGAAG AAAAAAAAGA CAGCGATGAT GAGCGATCAG ATAGGAATAA GCCTTGGTGG
    AAGAAGCGGT TTGTGTCTGC AATGCCCAAA GCTCCTATCC CATTTAGGAA GAAGGAAAAA
    CACGAGAAGG AGAGGGAAGA CTTGGTACAA GACAGGTTTT CAGTAGCAGA TGACTCAACA
    TCTCAGCTAG GGCCACATTC CCAGGCAGCA GAAGACATCC TGGCTAAGTA CAGGAATGCT
    ATTAAGAGGA CAATCCCCAA TGAAGGTTCC TCCGTGCCCT ATGAAGGAGC AGATGCTGTT
    GGAGATGGAG AAAGCCTTCA TGATTCTCCT CGTGATGAAG CTTTGCAGAA CATGGCCTCA
    GATGACCTGC TTGATTCTGC CAGCCAAGTA GCCCACCCAC AGGAGCCTGC ATTCTCCTAT
    AGGGATGCTA AGAAAAAATT ACGCCTGGCC CTGTGCTCTG CGGATTCAGT AGCGTTCCCT
    GTATTGTCTC ATTCGACACG GAATGGCCTG CCAGATCACA CAGACCCAGA AGACAATGAG
    ATTGTGTGCT TCCTTAAAGT CCAACTTGCA GAGGCCATAA ACCTGCAGGA CAAAAACCTG
    ATGGCTCAGA TCCAGGAAAC TATGCGATGT GTGAGCCGAT TTGACAGCCG CTCCTGCAGC
    AAAATTTTGG CATCCATTGC AGAGGATTAC CGGAAGAGGG CCTCATATAT TGCATATCTA
    ACAAGATGCC GACAGGGACT ACAGACCACC CAGGCTCACC TTGAGCGCCT CCTGCAGAGA
    GTGCTACGAG ATAAAGAGGT CTCCACGCGC TACTTCACCA CTGTCTGTGT GAGGCTTCTA
    CTTGAGAGCA AAGAGAAGGA AATCCATGAA TTTATCCAAG ATTTTCAAAA GTTAACTGCA
    GCTGATGATA AAACAGCACA AGTTGAAGAG TTCTTGCAAT CACTTTACGG GGCTATGGCA
    CAAGATGTGA TTTGGCAAAA TGCCAGTGAG GAGCAGCTGC AAGATGCACA GATTGCTATT
    GAGCGAAGCA TTATGAACCG CATCTTTAAG CTGGCCTTCT ATCCCAATCA AGATGCAGAT
    ATTCTGCGAG ATCAGGTCCT GCATGAACAC ATTAAAAGGT TGTCTAAAGT GGTGACTGCC
    AATCACAGGG CACTGCAGAT ACCTGAGGTG TATCTGAGGG AGGCTCCTTG GCCATCTGCC
    CAAACCGAAA TCCGTACCAT CAGCGCATAC AAGACACCCA GGGACAAAGT GCAGTGCATA
    TTGCGCATGT GCTCAACAAT CATGAACCTT CTAAGCCTGG CCAATGAGTA CTCTGTCCCA
    GGAGCAGATG ATTTTGTTCC TGTGCTGGTT TTTGTCCTTA TTAAGGCTAA TCCCCCCTGC
    CTACTATCAA CAGTGCAATA TATCAGCAAC TTCTATTCCA ATCGGCTCAC TGGCGAGGAG
    TCCTACTGGT GGATGCAGTT CACAGCTGCT GTTGAGTTCA TTAAAACCAT TGACGATCGC
    AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916795.1
    CDS161..4666
    Translation

    Target ORF information:

    RefSeq Version XM_004916738.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916738.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGGTC CTTCAAATCG TTCCAACTCA
    GTTTCCTCTT TGGATCTGGA AGGGGAGTCT GTATCTGAGC TAGGAGGAGG ACCATCCGGA
    AGCAATGGGG TGGAGGCTTT GCAGTTATTG GAACATGAAC AAGCTACCAC ACAGGATAAT
    CTAGATGACA AACTGCGCAA ATTTGAAATT CGAGACATGA TGGGATTGAC GGATGATAGA
    GATATATCAG AGACAGTCAG TGAGACATGG AGCACGGATG TGCTTGGAAG TGATTTTGAC
    CCCAACATTG ATGAAGATCG ACTACAAGAA ATTGCAGGTG CAGCAGCTGA GAACATGCTG
    GGAAGCCTGC TGTGCCTTCC CGGCTCTGGC TCAATACTGC TTGATCCTTG TACTGGATCC
    ACTATATCTG AGACTACCAG TGAAGCATGG AGTGTTGAGG TGCTCCCTAG TGATTCTGAA
    GCACCAGATC TAAAGCAAGA GGAGCGGCTG CAGGAACTGG AGAGCTGTTC AGGTTTGGGA
    AGCACATCAG ATGACACTGA AGTTAGAGAG GTCAGCTCCC GGCCCAGCAC TCCAGGACTC
    AGTGTGGTGT CAGGTGACTT TCCCAAACCT TTCTATTCAT TGCCGGGTAT CAGTGCTACC
    TCAGAGGACA TCCCAAACAA GATAGAGGAC CTACGCTCAG AGTGCAGCTC TGACTTTGGA
    GGAAAGGATT CTGTTACAAG TCCTGAGGCA GAAGAGTCTG TTCATGGACC TCACCATATA
    ACTGCACCTC CAACCCAGAC TGAATCCTTG TTGGCCATGT TTGACCCACT AGCTCCAGCA
    TCCAATGAAG TGGTTCGACC TAAAGTACAC TATGCGAGAC CCTCACATCC GCCTCCTGAC
    CCGCCTGTAC TGGAAGGAGC AAGTGGGGGA AATGAAGCTC GTCTTCCTAT GTTCGGTTCT
    CATGTATTTG TTCCCATGGA GTCTGAAGCT TACAGGCAGA GACACTCATG TCCTGAAAGA
    CTGGTGCGCA GTCGCAGCTC CGACATCGCT TCTTCCATAC GGAGGCCAAT GAGTGACCCT
    GGCTGGGTGC GGCGTGGGGT GAATGAAGAT AGGGAGCTGC CAACAGGAAC TGCAATTAAT
    GCACTAATTA ATCCCCCCCA GGCTTCATCC TCCTCACCAA GCAAGGACTC TTCCAGGGGA
    GAGCCAGAAG AAAAAAAAGA CAGCGATGAT GAGCGATCAG ATAGGAATAA GCCTTGGTGG
    AAGAAGCGGT TTGTGTCTGC AATGCCCAAA GCTCCTATCC CATTTAGGAA GAAGGAAAAA
    CACGAGAAGG AGAGGGAAGA CTTGGTACAA GACAGGTTTT CAGTAGCAGA TGACTCAACA
    TCTCAGCTAG GGCCACATTC CCAGGCAGCA GAAGACATCC TGGCTAAGTA CAGGAATGCT
    ATTAAGAGGA CAATCCCCAA TGAAGGTTCC TCCGTGCCCT ATGAAGGAGC AGATGCTGTT
    GGAGATGGAG AAAGCCTTCA TGATTCTCCT CGTGATGAAG CTTTGCAGAA CATGGCCTCA
    GATGACCTGC TTGATTCTGC CAGCCAAGTA GCCCACCCAC AGGAGCCTGC ATTCTCCTAT
    AGGGATGCTA AGAAAAAATT ACGCCTGGCC CTGTGCTCTG CGGATTCAGT AGCGTTCCCT
    GTATTGTCTC ATTCGACACG GAATGGCCTG CCAGATCACA CAGACCCAGA AGACAATGAG
    ATTGTGTGCT TCCTTAAAGT CCAACTTGCA GAGGCCATAA ACCTGCAGGA CAAAAACCTG
    ATGGCTCAGA TCCAGGAAAC TATGCGATGT GTGAGCCGAT TTGACAGCCG CTCCTGCAGC
    AAAATTTTGG CATCCATTGC AGAGGATTAC CGGAAGAGGG CCTCATATAT TGCATATCTA
    ACAAGATGCC GACAGGGACT ACAGACCACC CAGGCTCACC TTGAGCGCCT CCTGCAGAGA
    GTGCTACGAG ATAAAGAGGT CTCCACGCGC TACTTCACCA CTGTCTGTGT GAGGCTTCTA
    CTTGAGAGCA AAGAGAAGGA AATCCATGAA TTTATCCAAG ATTTTCAAAA GTTAACTGCA
    GCTGATGATA AAACAGCACA AGTTGAAGAG TTCTTGCAAT CACTTTACGG GGCTATGGCA
    CAAGATGTGA TTTGGCAAAA TGCCAGTGAG GAGCAGCTGC AAGATGCACA GATTGCTATT
    GAGCGAAGCA TTATGAACCG CATCTTTAAG CTGGCCTTCT ATCCCAATCA AGATGCAGAT
    ATTCTGCGAG ATCAGGTCCT GCATGAACAC ATTAAAAGGT TGTCTAAAGT GGTGACTGCC
    AATCACAGGG CACTGCAGAT ACCTGAGGTG TATCTGAGGG AGGCTCCTTG GCCATCTGCC
    CAAACCGAAA TCCGTACCAT CAGCGCATAC AAGACACCCA GGGACAAAGT GCAGTGCATA
    TTGCGCATGT GCTCAACAAT CATGAACCTT CTAAGCCTGG CCAATGAGTA CTCTGTCCCA
    GGAGCAGATG ATTTTGTTCC TGTGCTGGTT TTTGTCCTTA TTAAGGCTAA TCCCCCCTGC
    CTACTATCAA CAGTGCAATA TATCAGCAAC TTCTATTCCA ATCGGCTCAC TGGCGAGGAG
    TCCTACTGGT GGATGCAGTT CACAGCTGCT GTTGAGTTCA TTAAAACCAT TGACGATCGC
    AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31121
    Clone ID Related Accession (Same CDS sequence) XM_031891620.1
    Accession Version XM_031891620.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGGTC CTTCAAATCG TTCCAACTCA
    GTTTCCTCTT TGGATCTGGA AGGGGAGTCT GTATCTGAGC TAGGAGGAGG ACCATCCGGA
    AGCAATGGGG TGGAGGCTTT GCAGTTATTG GAACATGAAC AAGCTACCAC ACAGGATAAT
    CTAGATGACA AACTGCGCAA ATTTGAAATT CGAGACATGA TGGGATTGAC GGATGATAGA
    GATATATCAG AGACAGTCAG TGAGACATGG AGCACGGATG TGCTTGGAAG TGATTTTGAC
    CCCAACATTG ATGAAGATCG ACTACAAGAA ATTGCAGGTG CAGCAGCTGA GAACATGCTG
    GGAAGCCTGC TGTGCCTTCC CGGCTCTGGC TCAATACTGC TTGATCCTTG TACTGGATCC
    ACTATATCTG AGACTACCAG TGAAGCATGG AGTGTTGAGG TGCTCCCTAG TGATTCTGCA
    CCAGATCTAA AGCAAGAGGA GCGGCTGCAG GAACTGGAGA GCTGTTCAGG TTTGGGAAGC
    ACATCAGATG ACACTGAAGT TAGAGAGGTC AGCTCCCGGC CCAGCACTCC AGGACTCAGT
    GTGGTGTCAG GTGACTTTCC CAAACCTTTC TATTCATTGC CGGGTATCAG TGCTACCTCA
    GAGGACATCC CAAACAAGAT AGAGGACCTA CGCTCAGAGT GCAGCTCTGA CTTTGGAGGA
    AAGGATTCTG TTACAAGTCC TGAGGCAGAA GAGTCTGTTC ATGGACCTCA CCATATAACT
    GCACCTCCAA CCCAGACTGA ATCCTTGTTG GCCATGTTTG ACCCACTAGC TCCAGCATCC
    AATGAAGTGG TTCGACCTAA AGTACACTAT GCGAGACCCT CACATCCGCC TCCTGACCCG
    CCTGTACTGG AAGGAGCAAG TGGGGGAAAT GAAGCTCGTC TTCCTATGTT CGGTTCTCAT
    GTATTTGTTC CCATGGAGTC TGAAGCTTAC AGGCAGAGAC ACTCATGTCC TGAAAGACTG
    GTGCGCAGTC GCAGCTCCGA CATCGCTTCT TCCATACGGA GGCCAATGAG TGACCCTGGC
    TGGGTGCGGC GTGGGGTGAA TGAAGATAGG GAGCTGCCAA CAGGAACTGC AATTAATGCA
    CTAATTAATC CCCCCCAGGC TTCATCCTCC TCACCAAGCA AGGACTCTTC CAGGGGAGAG
    CCAGAAGAAA AAAAAGACAG CGATGATGAG CGATCAGATA GGAATAAGCC TTGGTGGAAG
    AAGCGGTTTG TGTCTGCAAT GCCCAAAGCT CCTATCCCAT TTAGGAAGAA GGAAAAACAC
    GAGAAGGAGA GGGAAGACTT GGTACAAGAC AGGTTTTCAG TAGCAGATGA CTCAACATCT
    CAGCTAGGGC CACATTCCCA GGCAGCAGAA GACATCCTGG CTAAGTACAG GAATGCTATT
    AAGAGGACAA TCCCCAATGA AGGTTCCTCC GTGCCCTATG AAGGAGCAGA TGCTGTTGGA
    GATGGAGAAA GCCTTCATGA TTCTCCTCGT GATGAAGCTT TGCAGAACAT GGCCTCAGAT
    GACCTGCTTG ATTCTGCCAG CCAAGTAGCC CACCCACAGG AGCCTGCATT CTCCTATAGG
    GATGCTAAGA AAAAATTACG CCTGGCCCTG TGCTCTGCGG ATTCAGTAGC GTTCCCTGTA
    TTGTCTCATT CGACACGGAA TGGCCTGCCA GATCACACAG ACCCAGAAGA CAATGAGATT
    GTGTGCTTCC TTAAAGTCCA ACTTGCAGAG GCCATAAACC TGCAGGACAA AAACCTGATG
    GCTCAGATCC AGGAAACTAT GCGATGTGTG AGCCGATTTG ACAGCCGCTC CTGCAGCAAA
    ATTTTGGCAT CCATTGCAGA GGATTACCGG AAGAGGGCCT CATATATTGC ATATCTAACA
    AGATGCCGAC AGGGACTACA GACCACCCAG GCTCACCTTG AGCGCCTCCT GCAGAGAGTG
    CTACGAGATA AAGAGGTCTC CACGCGCTAC TTCACCACTG TCTGTGTGAG GCTTCTACTT
    GAGAGCAAAG AGAAGGAAAT CCATGAATTT ATCCAAGATT TTCAAAAGTT AACTGCAGCT
    GATGATAAAA CAGCACAAGT TGAAGAGTTC TTGCAATCAC TTTACGGGGC TATGGCACAA
    GATGTGATTT GGCAAAATGC CAGTGAGGAG CAGCTGCAAG ATGCACAGAT TGCTATTGAG
    CGAAGCATTA TGAACCGCAT CTTTAAGCTG GCCTTCTATC CCAATCAAGA TGCAGATATT
    CTGCGAGATC AGGTCCTGCA TGAACACATT AAAAGGTTGT CTAAAGTGGT GACTGCCAAT
    CACAGGGCAC TGCAGATACC TGAGGTGTAT CTGAGGGAGG CTCCTTGGCC ATCTGCCCAA
    ACCGAAATCC GTACCATCAG CGCATACAAG ACACCCAGGG ACAAAGTGCA GTGCATATTG
    CGCATGTGCT CAACAATCAT GAACCTTCTA AGCCTGGCCA ATGAGTACTC TGTCCCAGGA
    GCAGATGATT TTGTTCCTGT GCTGGTTTTT GTCCTTATTA AGGCTAATCC CCCCTGCCTA
    CTATCAACAG TGCAATATAT CAGCAACTTC TATTCCAATC GGCTCACTGG CGAGGAGTCC
    TACTGGTGGA TGCAGTTCAC AGCTGCTGTT GAGTTCATTA AAACCATTGA CGATCGCAAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031747480.1
    CDS162..4664
    Translation

    Target ORF information:

    RefSeq Version XM_031891620.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031891620.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGGTC CTTCAAATCG TTCCAACTCA
    GTTTCCTCTT TGGATCTGGA AGGGGAGTCT GTATCTGAGC TAGGAGGAGG ACCATCCGGA
    AGCAATGGGG TGGAGGCTTT GCAGTTATTG GAACATGAAC AAGCTACCAC ACAGGATAAT
    CTAGATGACA AACTGCGCAA ATTTGAAATT CGAGACATGA TGGGATTGAC GGATGATAGA
    GATATATCAG AGACAGTCAG TGAGACATGG AGCACGGATG TGCTTGGAAG TGATTTTGAC
    CCCAACATTG ATGAAGATCG ACTACAAGAA ATTGCAGGTG CAGCAGCTGA GAACATGCTG
    GGAAGCCTGC TGTGCCTTCC CGGCTCTGGC TCAATACTGC TTGATCCTTG TACTGGATCC
    ACTATATCTG AGACTACCAG TGAAGCATGG AGTGTTGAGG TGCTCCCTAG TGATTCTGCA
    CCAGATCTAA AGCAAGAGGA GCGGCTGCAG GAACTGGAGA GCTGTTCAGG TTTGGGAAGC
    ACATCAGATG ACACTGAAGT TAGAGAGGTC AGCTCCCGGC CCAGCACTCC AGGACTCAGT
    GTGGTGTCAG GTGACTTTCC CAAACCTTTC TATTCATTGC CGGGTATCAG TGCTACCTCA
    GAGGACATCC CAAACAAGAT AGAGGACCTA CGCTCAGAGT GCAGCTCTGA CTTTGGAGGA
    AAGGATTCTG TTACAAGTCC TGAGGCAGAA GAGTCTGTTC ATGGACCTCA CCATATAACT
    GCACCTCCAA CCCAGACTGA ATCCTTGTTG GCCATGTTTG ACCCACTAGC TCCAGCATCC
    AATGAAGTGG TTCGACCTAA AGTACACTAT GCGAGACCCT CACATCCGCC TCCTGACCCG
    CCTGTACTGG AAGGAGCAAG TGGGGGAAAT GAAGCTCGTC TTCCTATGTT CGGTTCTCAT
    GTATTTGTTC CCATGGAGTC TGAAGCTTAC AGGCAGAGAC ACTCATGTCC TGAAAGACTG
    GTGCGCAGTC GCAGCTCCGA CATCGCTTCT TCCATACGGA GGCCAATGAG TGACCCTGGC
    TGGGTGCGGC GTGGGGTGAA TGAAGATAGG GAGCTGCCAA CAGGAACTGC AATTAATGCA
    CTAATTAATC CCCCCCAGGC TTCATCCTCC TCACCAAGCA AGGACTCTTC CAGGGGAGAG
    CCAGAAGAAA AAAAAGACAG CGATGATGAG CGATCAGATA GGAATAAGCC TTGGTGGAAG
    AAGCGGTTTG TGTCTGCAAT GCCCAAAGCT CCTATCCCAT TTAGGAAGAA GGAAAAACAC
    GAGAAGGAGA GGGAAGACTT GGTACAAGAC AGGTTTTCAG TAGCAGATGA CTCAACATCT
    CAGCTAGGGC CACATTCCCA GGCAGCAGAA GACATCCTGG CTAAGTACAG GAATGCTATT
    AAGAGGACAA TCCCCAATGA AGGTTCCTCC GTGCCCTATG AAGGAGCAGA TGCTGTTGGA
    GATGGAGAAA GCCTTCATGA TTCTCCTCGT GATGAAGCTT TGCAGAACAT GGCCTCAGAT
    GACCTGCTTG ATTCTGCCAG CCAAGTAGCC CACCCACAGG AGCCTGCATT CTCCTATAGG
    GATGCTAAGA AAAAATTACG CCTGGCCCTG TGCTCTGCGG ATTCAGTAGC GTTCCCTGTA
    TTGTCTCATT CGACACGGAA TGGCCTGCCA GATCACACAG ACCCAGAAGA CAATGAGATT
    GTGTGCTTCC TTAAAGTCCA ACTTGCAGAG GCCATAAACC TGCAGGACAA AAACCTGATG
    GCTCAGATCC AGGAAACTAT GCGATGTGTG AGCCGATTTG ACAGCCGCTC CTGCAGCAAA
    ATTTTGGCAT CCATTGCAGA GGATTACCGG AAGAGGGCCT CATATATTGC ATATCTAACA
    AGATGCCGAC AGGGACTACA GACCACCCAG GCTCACCTTG AGCGCCTCCT GCAGAGAGTG
    CTACGAGATA AAGAGGTCTC CACGCGCTAC TTCACCACTG TCTGTGTGAG GCTTCTACTT
    GAGAGCAAAG AGAAGGAAAT CCATGAATTT ATCCAAGATT TTCAAAAGTT AACTGCAGCT
    GATGATAAAA CAGCACAAGT TGAAGAGTTC TTGCAATCAC TTTACGGGGC TATGGCACAA
    GATGTGATTT GGCAAAATGC CAGTGAGGAG CAGCTGCAAG ATGCACAGAT TGCTATTGAG
    CGAAGCATTA TGAACCGCAT CTTTAAGCTG GCCTTCTATC CCAATCAAGA TGCAGATATT
    CTGCGAGATC AGGTCCTGCA TGAACACATT AAAAGGTTGT CTAAAGTGGT GACTGCCAAT
    CACAGGGCAC TGCAGATACC TGAGGTGTAT CTGAGGGAGG CTCCTTGGCC ATCTGCCCAA
    ACCGAAATCC GTACCATCAG CGCATACAAG ACACCCAGGG ACAAAGTGCA GTGCATATTG
    CGCATGTGCT CAACAATCAT GAACCTTCTA AGCCTGGCCA ATGAGTACTC TGTCCCAGGA
    GCAGATGATT TTGTTCCTGT GCTGGTTTTT GTCCTTATTA AGGCTAATCC CCCCTGCCTA
    CTATCAACAG TGCAATATAT CAGCAACTTC TATTCCAATC GGCTCACTGG CGAGGAGTCC
    TACTGGTGGA TGCAGTTCAC AGCTGCTGTT GAGTTCATTA AAACCATTGA CGATCGCAAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46314
    Clone ID Related Accession (Same CDS sequence) XM_018096652.1
    Accession Version XM_018096652.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGAAG AGGAAAATCC GTGTTCAACA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGAAGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTG ACTTTCCCAA ACCTTTCTAT
    TCATTGCCGG GTATCAGTGC TACCTCAGAG GACATCCCAA ACAAGATAGA GGACCTACGC
    TCAGAGTGCA GCTCTGACTT TGGAGGAAAG GATTCTGTTA CAAGTCCTGA GGCAGAAGAG
    TCTGTTCATG GACCTCACCA TATAACTGCA CCTCCAACCC AGACTGAATC CTTGTTGGCC
    ATGTTTGACC CACTAGCTCC AGCATCCAAT GAAGTGGTTC GACCTAAAGT ACACTATGCG
    AGACCCTCAC ATCCGCCTCC TGACCCGCCT GTACTGGAAG GAGCAAGTGG GGGAAATGAA
    GCTCGTCTTC CTATGTTCGG TTCTCATGTA TTTGTTCCCA TGGAGTCTGA AGCTTACAGG
    CAGAGACACT CATGTCCTGA AAGACTGGTG CGCAGTCGCA GCTCCGACAT CGCTTCTTCC
    ATACGGAGGC CAATGAGTGA CCCTGGCTGG GTGCGGCGTG GGGTGAATGA AGATAGGGAG
    CTGCCAACAG GAACTGCAAT TAATGCACTA ATTAATCCCC CCCAGGCTTC ATCCTCCTCA
    CCAAGCAAGG ACTCTTCCAG GGGAGAGCCA GAAGAAAAAA AAGACAGCGA TGATGAGCGA
    TCAGATAGGA ATAAGCCTTG GTGGAAGAAG CGGTTTGTGT CTGCAATGCC CAAAGCTCCT
    ATCCCATTTA GGAAGAAGGA AAAACACGAG AAGGAGAGGG AAGACTTGGT ACAAGACAGG
    TTTTCAGTAG CAGATGACTC AACATCTCAG CTAGGGCCAC ATTCCCAGGC AGCAGAAGAC
    ATCCTGGCTA AGTACAGGAA TGCTATTAAG AGGACAATCC CCAATGAAGG TTCCTCCGTG
    CCCTATGAAG GAGCAGATGC TGTTGGAGAT GGAGAAAGCC TTCATGATTC TCCTCGTGAT
    GAAGCTTTGC AGAACATGGC CTCAGATGAC CTGCTTGATT CTGCCAGCCA AGTAGCCCAC
    CCACAGGAGC CTGCATTCTC CTATAGGGAT GCTAAGAAAA AATTACGCCT GGCCCTGTGC
    TCTGCGGATT CAGTAGCGTT CCCTGTATTG TCTCATTCGA CACGGAATGG CCTGCCAGAT
    CACACAGACC CAGAAGACAA TGAGATTGTG TGCTTCCTTA AAGTCCAACT TGCAGAGGCC
    ATAAACCTGC AGGACAAAAA CCTGATGGCT CAGATCCAGG AAACTATGCG ATGTGTGAGC
    CGATTTGACA GCCGCTCCTG CAGCAAAATT TTGGCATCCA TTGCAGAGGA TTACCGGAAG
    AGGGCCTCAT ATATTGCATA TCTAACAAGA TGCCGACAGG GACTACAGAC CACCCAGGCT
    CACCTTGAGC GCCTCCTGCA GAGAGTGCTA CGAGATAAAG AGGTCTCCAC GCGCTACTTC
    ACCACTGTCT GTGTGAGGCT TCTACTTGAG AGCAAAGAGA AGGAAATCCA TGAATTTATC
    CAAGATTTTC AAAAGTTAAC TGCAGCTGAT GATAAAACAG CACAAGTTGA AGAGTTCTTG
    CAATCACTTT ACGGGGCTAT GGCACAAGAT GTGATTTGGC AAAATGCCAG TGAGGAGCAG
    CTGCAAGATG CACAGATTGC TATTGAGCGA AGCATTATGA ACCGCATCTT TAAGCTGGCC
    TTCTATCCCA ATCAAGATGC AGATATTCTG CGAGATCAGG TCCTGCATGA ACACATTAAA
    AGGTTGTCTA AAGTGGTGAC TGCCAATCAC AGGGCACTGC AGATACCTGA GGTGTATCTG
    AGGGAGGCTC CTTGGCCATC TGCCCAAACC GAAATCCGTA CCATCAGCGC ATACAAGACA
    CCCAGGGACA AAGTGCAGTG CATATTGCGC ATGTGCTCAA CAATCATGAA CCTTCTAAGC
    CTGGCCAATG AGTACTCTGT CCCAGGAGCA GATGATTTTG TTCCTGTGCT GGTTTTTGTC
    CTTATTAAGG CTAATCCCCC CTGCCTACTA TCAACAGTGC AATATATCAG CAACTTCTAT
    TCCAATCGGC TCACTGGCGA GGAGTCCTAC TGGTGGATGC AGTTCACAGC TGCTGTTGAG
    TTCATTAAAA CCATTGACGA TCGCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952141.1
    CDS171..4700
    Translation

    Target ORF information:

    RefSeq Version XM_018096652.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096652.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGAAG AGGAAAATCC GTGTTCAACA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGAAGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTG ACTTTCCCAA ACCTTTCTAT
    TCATTGCCGG GTATCAGTGC TACCTCAGAG GACATCCCAA ACAAGATAGA GGACCTACGC
    TCAGAGTGCA GCTCTGACTT TGGAGGAAAG GATTCTGTTA CAAGTCCTGA GGCAGAAGAG
    TCTGTTCATG GACCTCACCA TATAACTGCA CCTCCAACCC AGACTGAATC CTTGTTGGCC
    ATGTTTGACC CACTAGCTCC AGCATCCAAT GAAGTGGTTC GACCTAAAGT ACACTATGCG
    AGACCCTCAC ATCCGCCTCC TGACCCGCCT GTACTGGAAG GAGCAAGTGG GGGAAATGAA
    GCTCGTCTTC CTATGTTCGG TTCTCATGTA TTTGTTCCCA TGGAGTCTGA AGCTTACAGG
    CAGAGACACT CATGTCCTGA AAGACTGGTG CGCAGTCGCA GCTCCGACAT CGCTTCTTCC
    ATACGGAGGC CAATGAGTGA CCCTGGCTGG GTGCGGCGTG GGGTGAATGA AGATAGGGAG
    CTGCCAACAG GAACTGCAAT TAATGCACTA ATTAATCCCC CCCAGGCTTC ATCCTCCTCA
    CCAAGCAAGG ACTCTTCCAG GGGAGAGCCA GAAGAAAAAA AAGACAGCGA TGATGAGCGA
    TCAGATAGGA ATAAGCCTTG GTGGAAGAAG CGGTTTGTGT CTGCAATGCC CAAAGCTCCT
    ATCCCATTTA GGAAGAAGGA AAAACACGAG AAGGAGAGGG AAGACTTGGT ACAAGACAGG
    TTTTCAGTAG CAGATGACTC AACATCTCAG CTAGGGCCAC ATTCCCAGGC AGCAGAAGAC
    ATCCTGGCTA AGTACAGGAA TGCTATTAAG AGGACAATCC CCAATGAAGG TTCCTCCGTG
    CCCTATGAAG GAGCAGATGC TGTTGGAGAT GGAGAAAGCC TTCATGATTC TCCTCGTGAT
    GAAGCTTTGC AGAACATGGC CTCAGATGAC CTGCTTGATT CTGCCAGCCA AGTAGCCCAC
    CCACAGGAGC CTGCATTCTC CTATAGGGAT GCTAAGAAAA AATTACGCCT GGCCCTGTGC
    TCTGCGGATT CAGTAGCGTT CCCTGTATTG TCTCATTCGA CACGGAATGG CCTGCCAGAT
    CACACAGACC CAGAAGACAA TGAGATTGTG TGCTTCCTTA AAGTCCAACT TGCAGAGGCC
    ATAAACCTGC AGGACAAAAA CCTGATGGCT CAGATCCAGG AAACTATGCG ATGTGTGAGC
    CGATTTGACA GCCGCTCCTG CAGCAAAATT TTGGCATCCA TTGCAGAGGA TTACCGGAAG
    AGGGCCTCAT ATATTGCATA TCTAACAAGA TGCCGACAGG GACTACAGAC CACCCAGGCT
    CACCTTGAGC GCCTCCTGCA GAGAGTGCTA CGAGATAAAG AGGTCTCCAC GCGCTACTTC
    ACCACTGTCT GTGTGAGGCT TCTACTTGAG AGCAAAGAGA AGGAAATCCA TGAATTTATC
    CAAGATTTTC AAAAGTTAAC TGCAGCTGAT GATAAAACAG CACAAGTTGA AGAGTTCTTG
    CAATCACTTT ACGGGGCTAT GGCACAAGAT GTGATTTGGC AAAATGCCAG TGAGGAGCAG
    CTGCAAGATG CACAGATTGC TATTGAGCGA AGCATTATGA ACCGCATCTT TAAGCTGGCC
    TTCTATCCCA ATCAAGATGC AGATATTCTG CGAGATCAGG TCCTGCATGA ACACATTAAA
    AGGTTGTCTA AAGTGGTGAC TGCCAATCAC AGGGCACTGC AGATACCTGA GGTGTATCTG
    AGGGAGGCTC CTTGGCCATC TGCCCAAACC GAAATCCGTA CCATCAGCGC ATACAAGACA
    CCCAGGGACA AAGTGCAGTG CATATTGCGC ATGTGCTCAA CAATCATGAA CCTTCTAAGC
    CTGGCCAATG AGTACTCTGT CCCAGGAGCA GATGATTTTG TTCCTGTGCT GGTTTTTGTC
    CTTATTAAGG CTAATCCCCC CTGCCTACTA TCAACAGTGC AATATATCAG CAACTTCTAT
    TCCAATCGGC TCACTGGCGA GGAGTCCTAC TGGTGGATGC AGTTCACAGC TGCTGTTGAG
    TTCATTAAAA CCATTGACGA TCGCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46315
    Clone ID Related Accession (Same CDS sequence) XM_018096653.1
    Accession Version XM_018096653.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4527bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGAAG AGGAAAATCC GTGTTCAACA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGCACCAGAT CTAAAGCAAG AGGAGCGGCT GCAGGAACTG
    GAGAGCTGTT CAGGTTTGGG AAGCACATCA GATGACACTG AAGTTAGAGA GGTCAGCTCC
    CGGCCCAGCA CTCCAGGACT CAGTGTGGTG TCAGGTGACT TTCCCAAACC TTTCTATTCA
    TTGCCGGGTA TCAGTGCTAC CTCAGAGGAC ATCCCAAACA AGATAGAGGA CCTACGCTCA
    GAGTGCAGCT CTGACTTTGG AGGAAAGGAT TCTGTTACAA GTCCTGAGGC AGAAGAGTCT
    GTTCATGGAC CTCACCATAT AACTGCACCT CCAACCCAGA CTGAATCCTT GTTGGCCATG
    TTTGACCCAC TAGCTCCAGC ATCCAATGAA GTGGTTCGAC CTAAAGTACA CTATGCGAGA
    CCCTCACATC CGCCTCCTGA CCCGCCTGTA CTGGAAGGAG CAAGTGGGGG AAATGAAGCT
    CGTCTTCCTA TGTTCGGTTC TCATGTATTT GTTCCCATGG AGTCTGAAGC TTACAGGCAG
    AGACACTCAT GTCCTGAAAG ACTGGTGCGC AGTCGCAGCT CCGACATCGC TTCTTCCATA
    CGGAGGCCAA TGAGTGACCC TGGCTGGGTG CGGCGTGGGG TGAATGAAGA TAGGGAGCTG
    CCAACAGGAA CTGCAATTAA TGCACTAATT AATCCCCCCC AGGCTTCATC CTCCTCACCA
    AGCAAGGACT CTTCCAGGGG AGAGCCAGAA GAAAAAAAAG ACAGCGATGA TGAGCGATCA
    GATAGGAATA AGCCTTGGTG GAAGAAGCGG TTTGTGTCTG CAATGCCCAA AGCTCCTATC
    CCATTTAGGA AGAAGGAAAA ACACGAGAAG GAGAGGGAAG ACTTGGTACA AGACAGGTTT
    TCAGTAGCAG ATGACTCAAC ATCTCAGCTA GGGCCACATT CCCAGGCAGC AGAAGACATC
    CTGGCTAAGT ACAGGAATGC TATTAAGAGG ACAATCCCCA ATGAAGGTTC CTCCGTGCCC
    TATGAAGGAG CAGATGCTGT TGGAGATGGA GAAAGCCTTC ATGATTCTCC TCGTGATGAA
    GCTTTGCAGA ACATGGCCTC AGATGACCTG CTTGATTCTG CCAGCCAAGT AGCCCACCCA
    CAGGAGCCTG CATTCTCCTA TAGGGATGCT AAGAAAAAAT TACGCCTGGC CCTGTGCTCT
    GCGGATTCAG TAGCGTTCCC TGTATTGTCT CATTCGACAC GGAATGGCCT GCCAGATCAC
    ACAGACCCAG AAGACAATGA GATTGTGTGC TTCCTTAAAG TCCAACTTGC AGAGGCCATA
    AACCTGCAGG ACAAAAACCT GATGGCTCAG ATCCAGGAAA CTATGCGATG TGTGAGCCGA
    TTTGACAGCC GCTCCTGCAG CAAAATTTTG GCATCCATTG CAGAGGATTA CCGGAAGAGG
    GCCTCATATA TTGCATATCT AACAAGATGC CGACAGGGAC TACAGACCAC CCAGGCTCAC
    CTTGAGCGCC TCCTGCAGAG AGTGCTACGA GATAAAGAGG TCTCCACGCG CTACTTCACC
    ACTGTCTGTG TGAGGCTTCT ACTTGAGAGC AAAGAGAAGG AAATCCATGA ATTTATCCAA
    GATTTTCAAA AGTTAACTGC AGCTGATGAT AAAACAGCAC AAGTTGAAGA GTTCTTGCAA
    TCACTTTACG GGGCTATGGC ACAAGATGTG ATTTGGCAAA ATGCCAGTGA GGAGCAGCTG
    CAAGATGCAC AGATTGCTAT TGAGCGAAGC ATTATGAACC GCATCTTTAA GCTGGCCTTC
    TATCCCAATC AAGATGCAGA TATTCTGCGA GATCAGGTCC TGCATGAACA CATTAAAAGG
    TTGTCTAAAG TGGTGACTGC CAATCACAGG GCACTGCAGA TACCTGAGGT GTATCTGAGG
    GAGGCTCCTT GGCCATCTGC CCAAACCGAA ATCCGTACCA TCAGCGCATA CAAGACACCC
    AGGGACAAAG TGCAGTGCAT ATTGCGCATG TGCTCAACAA TCATGAACCT TCTAAGCCTG
    GCCAATGAGT ACTCTGTCCC AGGAGCAGAT GATTTTGTTC CTGTGCTGGT TTTTGTCCTT
    ATTAAGGCTA ATCCCCCCTG CCTACTATCA ACAGTGCAAT ATATCAGCAA CTTCTATTCC
    AATCGGCTCA CTGGCGAGGA GTCCTACTGG TGGATGCAGT TCACAGCTGC TGTTGAGTTC
    ATTAAAACCA TTGACGATCG CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952142.1
    CDS172..4698
    Translation

    Target ORF information:

    RefSeq Version XM_018096653.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096653.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGAAG AGGAAAATCC GTGTTCAACA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGCACCAGAT CTAAAGCAAG AGGAGCGGCT GCAGGAACTG
    GAGAGCTGTT CAGGTTTGGG AAGCACATCA GATGACACTG AAGTTAGAGA GGTCAGCTCC
    CGGCCCAGCA CTCCAGGACT CAGTGTGGTG TCAGGTGACT TTCCCAAACC TTTCTATTCA
    TTGCCGGGTA TCAGTGCTAC CTCAGAGGAC ATCCCAAACA AGATAGAGGA CCTACGCTCA
    GAGTGCAGCT CTGACTTTGG AGGAAAGGAT TCTGTTACAA GTCCTGAGGC AGAAGAGTCT
    GTTCATGGAC CTCACCATAT AACTGCACCT CCAACCCAGA CTGAATCCTT GTTGGCCATG
    TTTGACCCAC TAGCTCCAGC ATCCAATGAA GTGGTTCGAC CTAAAGTACA CTATGCGAGA
    CCCTCACATC CGCCTCCTGA CCCGCCTGTA CTGGAAGGAG CAAGTGGGGG AAATGAAGCT
    CGTCTTCCTA TGTTCGGTTC TCATGTATTT GTTCCCATGG AGTCTGAAGC TTACAGGCAG
    AGACACTCAT GTCCTGAAAG ACTGGTGCGC AGTCGCAGCT CCGACATCGC TTCTTCCATA
    CGGAGGCCAA TGAGTGACCC TGGCTGGGTG CGGCGTGGGG TGAATGAAGA TAGGGAGCTG
    CCAACAGGAA CTGCAATTAA TGCACTAATT AATCCCCCCC AGGCTTCATC CTCCTCACCA
    AGCAAGGACT CTTCCAGGGG AGAGCCAGAA GAAAAAAAAG ACAGCGATGA TGAGCGATCA
    GATAGGAATA AGCCTTGGTG GAAGAAGCGG TTTGTGTCTG CAATGCCCAA AGCTCCTATC
    CCATTTAGGA AGAAGGAAAA ACACGAGAAG GAGAGGGAAG ACTTGGTACA AGACAGGTTT
    TCAGTAGCAG ATGACTCAAC ATCTCAGCTA GGGCCACATT CCCAGGCAGC AGAAGACATC
    CTGGCTAAGT ACAGGAATGC TATTAAGAGG ACAATCCCCA ATGAAGGTTC CTCCGTGCCC
    TATGAAGGAG CAGATGCTGT TGGAGATGGA GAAAGCCTTC ATGATTCTCC TCGTGATGAA
    GCTTTGCAGA ACATGGCCTC AGATGACCTG CTTGATTCTG CCAGCCAAGT AGCCCACCCA
    CAGGAGCCTG CATTCTCCTA TAGGGATGCT AAGAAAAAAT TACGCCTGGC CCTGTGCTCT
    GCGGATTCAG TAGCGTTCCC TGTATTGTCT CATTCGACAC GGAATGGCCT GCCAGATCAC
    ACAGACCCAG AAGACAATGA GATTGTGTGC TTCCTTAAAG TCCAACTTGC AGAGGCCATA
    AACCTGCAGG ACAAAAACCT GATGGCTCAG ATCCAGGAAA CTATGCGATG TGTGAGCCGA
    TTTGACAGCC GCTCCTGCAG CAAAATTTTG GCATCCATTG CAGAGGATTA CCGGAAGAGG
    GCCTCATATA TTGCATATCT AACAAGATGC CGACAGGGAC TACAGACCAC CCAGGCTCAC
    CTTGAGCGCC TCCTGCAGAG AGTGCTACGA GATAAAGAGG TCTCCACGCG CTACTTCACC
    ACTGTCTGTG TGAGGCTTCT ACTTGAGAGC AAAGAGAAGG AAATCCATGA ATTTATCCAA
    GATTTTCAAA AGTTAACTGC AGCTGATGAT AAAACAGCAC AAGTTGAAGA GTTCTTGCAA
    TCACTTTACG GGGCTATGGC ACAAGATGTG ATTTGGCAAA ATGCCAGTGA GGAGCAGCTG
    CAAGATGCAC AGATTGCTAT TGAGCGAAGC ATTATGAACC GCATCTTTAA GCTGGCCTTC
    TATCCCAATC AAGATGCAGA TATTCTGCGA GATCAGGTCC TGCATGAACA CATTAAAAGG
    TTGTCTAAAG TGGTGACTGC CAATCACAGG GCACTGCAGA TACCTGAGGT GTATCTGAGG
    GAGGCTCCTT GGCCATCTGC CCAAACCGAA ATCCGTACCA TCAGCGCATA CAAGACACCC
    AGGGACAAAG TGCAGTGCAT ATTGCGCATG TGCTCAACAA TCATGAACCT TCTAAGCCTG
    GCCAATGAGT ACTCTGTCCC AGGAGCAGAT GATTTTGTTC CTGTGCTGGT TTTTGTCCTT
    ATTAAGGCTA ATCCCCCCTG CCTACTATCA ACAGTGCAAT ATATCAGCAA CTTCTATTCC
    AATCGGCTCA CTGGCGAGGA GTCCTACTGG TGGATGCAGT TCACAGCTGC TGTTGAGTTC
    ATTAAAACCA TTGACGATCG CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46316
    Clone ID Related Accession (Same CDS sequence) XM_018096654.1
    Accession Version XM_018096654.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGAAG AGGAAAATCC GTGTTCAACA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGAAGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTG TTGCTCTTTC AGGTATCAGT
    GCTACCTCAG AGGACATCCC AAACAAGATA GAGGACCTAC GCTCAGAGTG CAGCTCTGAC
    TTTGGAGGAA AGGATTCTGT TACAAGTCCT GAGGCAGAAG AGTCTGTTCA TGGACCTCAC
    CATATAACTG CACCTCCAAC CCAGACTGAA TCCTTGTTGG CCATGTTTGA CCCACTAGCT
    CCAGCATCCA ATGAAGTGGT TCGACCTAAA GTACACTATG CGAGACCCTC ACATCCGCCT
    CCTGACCCGC CTGTACTGGA AGGAGCAAGT GGGGGAAATG AAGCTCGTCT TCCTATGTTC
    GGTTCTCATG TATTTGTTCC CATGGAGTCT GAAGCTTACA GGCAGAGACA CTCATGTCCT
    GAAAGACTGG TGCGCAGTCG CAGCTCCGAC ATCGCTTCTT CCATACGGAG GCCAATGAGT
    GACCCTGGCT GGGTGCGGCG TGGGGTGAAT GAAGATAGGG AGCTGCCAAC AGGAACTGCA
    ATTAATGCAC TAATTAATCC CCCCCAGGCT TCATCCTCCT CACCAAGCAA GGACTCTTCC
    AGGGGAGAGC CAGAAGAAAA AAAAGACAGC GATGATGAGC GATCAGATAG GAATAAGCCT
    TGGTGGAAGA AGCGGTTTGT GTCTGCAATG CCCAAAGCTC CTATCCCATT TAGGAAGAAG
    GAAAAACACG AGAAGGAGAG GGAAGACTTG GTACAAGACA GGTTTTCAGT AGCAGATGAC
    TCAACATCTC AGCTAGGGCC ACATTCCCAG GCAGCAGAAG ACATCCTGGC TAAGTACAGG
    AATGCTATTA AGAGGACAAT CCCCAATGAA GGTTCCTCCG TGCCCTATGA AGGAGCAGAT
    GCTGTTGGAG ATGGAGAAAG CCTTCATGAT TCTCCTCGTG ATGAAGCTTT GCAGAACATG
    GCCTCAGATG ACCTGCTTGA TTCTGCCAGC CAAGTAGCCC ACCCACAGGA GCCTGCATTC
    TCCTATAGGG ATGCTAAGAA AAAATTACGC CTGGCCCTGT GCTCTGCGGA TTCAGTAGCG
    TTCCCTGTAT TGTCTCATTC GACACGGAAT GGCCTGCCAG ATCACACAGA CCCAGAAGAC
    AATGAGATTG TGTGCTTCCT TAAAGTCCAA CTTGCAGAGG CCATAAACCT GCAGGACAAA
    AACCTGATGG CTCAGATCCA GGAAACTATG CGATGTGTGA GCCGATTTGA CAGCCGCTCC
    TGCAGCAAAA TTTTGGCATC CATTGCAGAG GATTACCGGA AGAGGGCCTC ATATATTGCA
    TATCTAACAA GATGCCGACA GGGACTACAG ACCACCCAGG CTCACCTTGA GCGCCTCCTG
    CAGAGAGTGC TACGAGATAA AGAGGTCTCC ACGCGCTACT TCACCACTGT CTGTGTGAGG
    CTTCTACTTG AGAGCAAAGA GAAGGAAATC CATGAATTTA TCCAAGATTT TCAAAAGTTA
    ACTGCAGCTG ATGATAAAAC AGCACAAGTT GAAGAGTTCT TGCAATCACT TTACGGGGCT
    ATGGCACAAG ATGTGATTTG GCAAAATGCC AGTGAGGAGC AGCTGCAAGA TGCACAGATT
    GCTATTGAGC GAAGCATTAT GAACCGCATC TTTAAGCTGG CCTTCTATCC CAATCAAGAT
    GCAGATATTC TGCGAGATCA GGTCCTGCAT GAACACATTA AAAGGTTGTC TAAAGTGGTG
    ACTGCCAATC ACAGGGCACT GCAGATACCT GAGGTGTATC TGAGGGAGGC TCCTTGGCCA
    TCTGCCCAAA CCGAAATCCG TACCATCAGC GCATACAAGA CACCCAGGGA CAAAGTGCAG
    TGCATATTGC GCATGTGCTC AACAATCATG AACCTTCTAA GCCTGGCCAA TGAGTACTCT
    GTCCCAGGAG CAGATGATTT TGTTCCTGTG CTGGTTTTTG TCCTTATTAA GGCTAATCCC
    CCCTGCCTAC TATCAACAGT GCAATATATC AGCAACTTCT ATTCCAATCG GCTCACTGGC
    GAGGAGTCCT ACTGGTGGAT GCAGTTCACA GCTGCTGTTG AGTTCATTAA AACCATTGAC
    GATCGCAAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952143.1
    CDS171..4682
    Translation

    Target ORF information:

    RefSeq Version XM_018096654.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096654.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGAAG AGGAAAATCC GTGTTCAACA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGAAGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTG TTGCTCTTTC AGGTATCAGT
    GCTACCTCAG AGGACATCCC AAACAAGATA GAGGACCTAC GCTCAGAGTG CAGCTCTGAC
    TTTGGAGGAA AGGATTCTGT TACAAGTCCT GAGGCAGAAG AGTCTGTTCA TGGACCTCAC
    CATATAACTG CACCTCCAAC CCAGACTGAA TCCTTGTTGG CCATGTTTGA CCCACTAGCT
    CCAGCATCCA ATGAAGTGGT TCGACCTAAA GTACACTATG CGAGACCCTC ACATCCGCCT
    CCTGACCCGC CTGTACTGGA AGGAGCAAGT GGGGGAAATG AAGCTCGTCT TCCTATGTTC
    GGTTCTCATG TATTTGTTCC CATGGAGTCT GAAGCTTACA GGCAGAGACA CTCATGTCCT
    GAAAGACTGG TGCGCAGTCG CAGCTCCGAC ATCGCTTCTT CCATACGGAG GCCAATGAGT
    GACCCTGGCT GGGTGCGGCG TGGGGTGAAT GAAGATAGGG AGCTGCCAAC AGGAACTGCA
    ATTAATGCAC TAATTAATCC CCCCCAGGCT TCATCCTCCT CACCAAGCAA GGACTCTTCC
    AGGGGAGAGC CAGAAGAAAA AAAAGACAGC GATGATGAGC GATCAGATAG GAATAAGCCT
    TGGTGGAAGA AGCGGTTTGT GTCTGCAATG CCCAAAGCTC CTATCCCATT TAGGAAGAAG
    GAAAAACACG AGAAGGAGAG GGAAGACTTG GTACAAGACA GGTTTTCAGT AGCAGATGAC
    TCAACATCTC AGCTAGGGCC ACATTCCCAG GCAGCAGAAG ACATCCTGGC TAAGTACAGG
    AATGCTATTA AGAGGACAAT CCCCAATGAA GGTTCCTCCG TGCCCTATGA AGGAGCAGAT
    GCTGTTGGAG ATGGAGAAAG CCTTCATGAT TCTCCTCGTG ATGAAGCTTT GCAGAACATG
    GCCTCAGATG ACCTGCTTGA TTCTGCCAGC CAAGTAGCCC ACCCACAGGA GCCTGCATTC
    TCCTATAGGG ATGCTAAGAA AAAATTACGC CTGGCCCTGT GCTCTGCGGA TTCAGTAGCG
    TTCCCTGTAT TGTCTCATTC GACACGGAAT GGCCTGCCAG ATCACACAGA CCCAGAAGAC
    AATGAGATTG TGTGCTTCCT TAAAGTCCAA CTTGCAGAGG CCATAAACCT GCAGGACAAA
    AACCTGATGG CTCAGATCCA GGAAACTATG CGATGTGTGA GCCGATTTGA CAGCCGCTCC
    TGCAGCAAAA TTTTGGCATC CATTGCAGAG GATTACCGGA AGAGGGCCTC ATATATTGCA
    TATCTAACAA GATGCCGACA GGGACTACAG ACCACCCAGG CTCACCTTGA GCGCCTCCTG
    CAGAGAGTGC TACGAGATAA AGAGGTCTCC ACGCGCTACT TCACCACTGT CTGTGTGAGG
    CTTCTACTTG AGAGCAAAGA GAAGGAAATC CATGAATTTA TCCAAGATTT TCAAAAGTTA
    ACTGCAGCTG ATGATAAAAC AGCACAAGTT GAAGAGTTCT TGCAATCACT TTACGGGGCT
    ATGGCACAAG ATGTGATTTG GCAAAATGCC AGTGAGGAGC AGCTGCAAGA TGCACAGATT
    GCTATTGAGC GAAGCATTAT GAACCGCATC TTTAAGCTGG CCTTCTATCC CAATCAAGAT
    GCAGATATTC TGCGAGATCA GGTCCTGCAT GAACACATTA AAAGGTTGTC TAAAGTGGTG
    ACTGCCAATC ACAGGGCACT GCAGATACCT GAGGTGTATC TGAGGGAGGC TCCTTGGCCA
    TCTGCCCAAA CCGAAATCCG TACCATCAGC GCATACAAGA CACCCAGGGA CAAAGTGCAG
    TGCATATTGC GCATGTGCTC AACAATCATG AACCTTCTAA GCCTGGCCAA TGAGTACTCT
    GTCCCAGGAG CAGATGATTT TGTTCCTGTG CTGGTTTTTG TCCTTATTAA GGCTAATCCC
    CCCTGCCTAC TATCAACAGT GCAATATATC AGCAACTTCT ATTCCAATCG GCTCACTGGC
    GAGGAGTCCT ACTGGTGGAT GCAGTTCACA GCTGCTGTTG AGTTCATTAA AACCATTGAC
    GATCGCAAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14180
    Clone ID Related Accession (Same CDS sequence) XM_004916738.3 , XM_004916738.4
    Accession Version XM_004916738.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916738.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGGTC CTTCAAATCG TTCCAACTCA
    GTTTCCTCTT TGGATCTGGA AGGGGAGTCT GTATCTGAGC TAGGAGGAGG ACCATCCGGA
    AGCAATGGGG TGGAGGCTTT GCAGTTATTG GAACATGAAC AAGCTACCAC ACAGGATAAT
    CTAGATGACA AACTGCGCAA ATTTGAAATT CGAGACATGA TGGGATTGAC GGATGATAGA
    GATATATCAG AGACAGTCAG TGAGACATGG AGCACGGATG TGCTTGGAAG TGATTTTGAC
    CCCAACATTG ATGAAGATCG ACTACAAGAA ATTGCAGGTG CAGCAGCTGA GAACATGCTG
    GGAAGCCTGC TGTGCCTTCC CGGCTCTGGC TCAATACTGC TTGATCCTTG TACTGGATCC
    ACTATATCTG AGACTACCAG TGAAGCATGG AGTGTTGAGG TGCTCCCTAG TGATTCTGAA
    GCACCAGATC TAAAGCAAGA GGAGCGGCTG CAGGAACTGG AGAGCTGTTC AGGTTTGGGA
    AGCACATCAG ATGACACTGA AGTTAGAGAG GTCAGCTCCC GGCCCAGCAC TCCAGGACTC
    AGTGTGGTGT CAGGTGACTT TCCCAAACCT TTCTATTCAT TGCCGGGTAT CAGTGCTACC
    TCAGAGGACA TCCCAAACAA GATAGAGGAC CTACGCTCAG AGTGCAGCTC TGACTTTGGA
    GGAAAGGATT CTGTTACAAG TCCTGAGGCA GAAGAGTCTG TTCATGGACC TCACCATATA
    ACTGCACCTC CAACCCAGAC TGAATCCTTG TTGGCCATGT TTGACCCACT AGCTCCAGCA
    TCCAATGAAG TGGTTCGACC TAAAGTACAC TATGCGAGAC CCTCACATCC GCCTCCTGAC
    CCGCCTGTAC TGGAAGGAGC AAGTGGGGGA AATGAAGCTC GTCTTCCTAT GTTCGGTTCT
    CATGTATTTG TTCCCATGGA GTCTGAAGCT TACAGGCAGA GACACTCATG TCCTGAAAGA
    CTGGTGCGCA GTCGCAGCTC CGACATCGCT TCTTCCATAC GGAGGCCAAT GAGTGACCCT
    GGCTGGGTGC GGCGTGGGGT GAATGAAGAT AGGGAGCTGC CAACAGGAAC TGCAATTAAT
    GCACTAATTA ATCCCCCCCA GGCTTCATCC TCCTCACCAA GCAAGGACTC TTCCAGGGGA
    GAGCCAGAAG AAAAAAAAGA CAGCGATGAT GAGCGATCAG ATAGGAATAA GCCTTGGTGG
    AAGAAGCGGT TTGTGTCTGC AATGCCCAAA GCTCCTATCC CATTTAGGAA GAAGGAAAAA
    CACGAGAAGG AGAGGGAAGA CTTGGTACAA GACAGGTTTT CAGTAGCAGA TGACTCAACA
    TCTCAGCTAG GGCCACATTC CCAGGCAGCA GAAGACATCC TGGCTAAGTA CAGGAATGCT
    ATTAAGAGGA CAATCCCCAA TGAAGGTTCC TCCGTGCCCT ATGAAGGAGC AGATGCTGTT
    GGAGATGGAG AAAGCCTTCA TGATTCTCCT CGTGATGAAG CTTTGCAGAA CATGGCCTCA
    GATGACCTGC TTGATTCTGC CAGCCAAGTA GCCCACCCAC AGGAGCCTGC ATTCTCCTAT
    AGGGATGCTA AGAAAAAATT ACGCCTGGCC CTGTGCTCTG CGGATTCAGT AGCGTTCCCT
    GTATTGTCTC ATTCGACACG GAATGGCCTG CCAGATCACA CAGACCCAGA AGACAATGAG
    ATTGTGTGCT TCCTTAAAGT CCAACTTGCA GAGGCCATAA ACCTGCAGGA CAAAAACCTG
    ATGGCTCAGA TCCAGGAAAC TATGCGATGT GTGAGCCGAT TTGACAGCCG CTCCTGCAGC
    AAAATTTTGG CATCCATTGC AGAGGATTAC CGGAAGAGGG CCTCATATAT TGCATATCTA
    ACAAGATGCC GACAGGGACT ACAGACCACC CAGGCTCACC TTGAGCGCCT CCTGCAGAGA
    GTGCTACGAG ATAAAGAGGT CTCCACGCGC TACTTCACCA CTGTCTGTGT GAGGCTTCTA
    CTTGAGAGCA AAGAGAAGGA AATCCATGAA TTTATCCAAG ATTTTCAAAA GTTAACTGCA
    GCTGATGATA AAACAGCACA AGTTGAAGAG TTCTTGCAAT CACTTTACGG GGCTATGGCA
    CAAGATGTGA TTTGGCAAAA TGCCAGTGAG GAGCAGCTGC AAGATGCACA GATTGCTATT
    GAGCGAAGCA TTATGAACCG CATCTTTAAG CTGGCCTTCT ATCCCAATCA AGATGCAGAT
    ATTCTGCGAG ATCAGGTCCT GCATGAACAC ATTAAAAGGT TGTCTAAAGT GGTGACTGCC
    AATCACAGGG CACTGCAGAT ACCTGAGGTG TATCTGAGGG AGGCTCCTTG GCCATCTGCC
    CAAACCGAAA TCCGTACCAT CAGCGCATAC AAGACACCCA GGGACAAAGT GCAGTGCATA
    TTGCGCATGT GCTCAACAAT CATGAACCTT CTAAGCCTGG CCAATGAGTA CTCTGTCCCA
    GGAGCAGATG ATTTTGTTCC TGTGCTGGTT TTTGTCCTTA TTAAGGCTAA TCCCCCCTGC
    CTACTATCAA CAGTGCAATA TATCAGCAAC TTCTATTCCA ATCGGCTCAC TGGCGAGGAG
    TCCTACTGGT GGATGCAGTT CACAGCTGCT GTTGAGTTCA TTAAAACCAT TGACGATCGC
    AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916795.1
    CDS171..4676
    Translation

    Target ORF information:

    RefSeq Version XM_004916738.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916738.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTGAATGTT TGGGTTTAAG AATAATTGAC GTTCCAGGTC CTTCAAATCG TTCCAACTCA
    GTTTCCTCTT TGGATCTGGA AGGGGAGTCT GTATCTGAGC TAGGAGGAGG ACCATCCGGA
    AGCAATGGGG TGGAGGCTTT GCAGTTATTG GAACATGAAC AAGCTACCAC ACAGGATAAT
    CTAGATGACA AACTGCGCAA ATTTGAAATT CGAGACATGA TGGGATTGAC GGATGATAGA
    GATATATCAG AGACAGTCAG TGAGACATGG AGCACGGATG TGCTTGGAAG TGATTTTGAC
    CCCAACATTG ATGAAGATCG ACTACAAGAA ATTGCAGGTG CAGCAGCTGA GAACATGCTG
    GGAAGCCTGC TGTGCCTTCC CGGCTCTGGC TCAATACTGC TTGATCCTTG TACTGGATCC
    ACTATATCTG AGACTACCAG TGAAGCATGG AGTGTTGAGG TGCTCCCTAG TGATTCTGAA
    GCACCAGATC TAAAGCAAGA GGAGCGGCTG CAGGAACTGG AGAGCTGTTC AGGTTTGGGA
    AGCACATCAG ATGACACTGA AGTTAGAGAG GTCAGCTCCC GGCCCAGCAC TCCAGGACTC
    AGTGTGGTGT CAGGTGACTT TCCCAAACCT TTCTATTCAT TGCCGGGTAT CAGTGCTACC
    TCAGAGGACA TCCCAAACAA GATAGAGGAC CTACGCTCAG AGTGCAGCTC TGACTTTGGA
    GGAAAGGATT CTGTTACAAG TCCTGAGGCA GAAGAGTCTG TTCATGGACC TCACCATATA
    ACTGCACCTC CAACCCAGAC TGAATCCTTG TTGGCCATGT TTGACCCACT AGCTCCAGCA
    TCCAATGAAG TGGTTCGACC TAAAGTACAC TATGCGAGAC CCTCACATCC GCCTCCTGAC
    CCGCCTGTAC TGGAAGGAGC AAGTGGGGGA AATGAAGCTC GTCTTCCTAT GTTCGGTTCT
    CATGTATTTG TTCCCATGGA GTCTGAAGCT TACAGGCAGA GACACTCATG TCCTGAAAGA
    CTGGTGCGCA GTCGCAGCTC CGACATCGCT TCTTCCATAC GGAGGCCAAT GAGTGACCCT
    GGCTGGGTGC GGCGTGGGGT GAATGAAGAT AGGGAGCTGC CAACAGGAAC TGCAATTAAT
    GCACTAATTA ATCCCCCCCA GGCTTCATCC TCCTCACCAA GCAAGGACTC TTCCAGGGGA
    GAGCCAGAAG AAAAAAAAGA CAGCGATGAT GAGCGATCAG ATAGGAATAA GCCTTGGTGG
    AAGAAGCGGT TTGTGTCTGC AATGCCCAAA GCTCCTATCC CATTTAGGAA GAAGGAAAAA
    CACGAGAAGG AGAGGGAAGA CTTGGTACAA GACAGGTTTT CAGTAGCAGA TGACTCAACA
    TCTCAGCTAG GGCCACATTC CCAGGCAGCA GAAGACATCC TGGCTAAGTA CAGGAATGCT
    ATTAAGAGGA CAATCCCCAA TGAAGGTTCC TCCGTGCCCT ATGAAGGAGC AGATGCTGTT
    GGAGATGGAG AAAGCCTTCA TGATTCTCCT CGTGATGAAG CTTTGCAGAA CATGGCCTCA
    GATGACCTGC TTGATTCTGC CAGCCAAGTA GCCCACCCAC AGGAGCCTGC ATTCTCCTAT
    AGGGATGCTA AGAAAAAATT ACGCCTGGCC CTGTGCTCTG CGGATTCAGT AGCGTTCCCT
    GTATTGTCTC ATTCGACACG GAATGGCCTG CCAGATCACA CAGACCCAGA AGACAATGAG
    ATTGTGTGCT TCCTTAAAGT CCAACTTGCA GAGGCCATAA ACCTGCAGGA CAAAAACCTG
    ATGGCTCAGA TCCAGGAAAC TATGCGATGT GTGAGCCGAT TTGACAGCCG CTCCTGCAGC
    AAAATTTTGG CATCCATTGC AGAGGATTAC CGGAAGAGGG CCTCATATAT TGCATATCTA
    ACAAGATGCC GACAGGGACT ACAGACCACC CAGGCTCACC TTGAGCGCCT CCTGCAGAGA
    GTGCTACGAG ATAAAGAGGT CTCCACGCGC TACTTCACCA CTGTCTGTGT GAGGCTTCTA
    CTTGAGAGCA AAGAGAAGGA AATCCATGAA TTTATCCAAG ATTTTCAAAA GTTAACTGCA
    GCTGATGATA AAACAGCACA AGTTGAAGAG TTCTTGCAAT CACTTTACGG GGCTATGGCA
    CAAGATGTGA TTTGGCAAAA TGCCAGTGAG GAGCAGCTGC AAGATGCACA GATTGCTATT
    GAGCGAAGCA TTATGAACCG CATCTTTAAG CTGGCCTTCT ATCCCAATCA AGATGCAGAT
    ATTCTGCGAG ATCAGGTCCT GCATGAACAC ATTAAAAGGT TGTCTAAAGT GGTGACTGCC
    AATCACAGGG CACTGCAGAT ACCTGAGGTG TATCTGAGGG AGGCTCCTTG GCCATCTGCC
    CAAACCGAAA TCCGTACCAT CAGCGCATAC AAGACACCCA GGGACAAAGT GCAGTGCATA
    TTGCGCATGT GCTCAACAAT CATGAACCTT CTAAGCCTGG CCAATGAGTA CTCTGTCCCA
    GGAGCAGATG ATTTTGTTCC TGTGCTGGTT TTTGTCCTTA TTAAGGCTAA TCCCCCCTGC
    CTACTATCAA CAGTGCAATA TATCAGCAAC TTCTATTCCA ATCGGCTCAC TGGCGAGGAG
    TCCTACTGGT GGATGCAGTT CACAGCTGCT GTTGAGTTCA TTAAAACCAT TGACGATCGC
    AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14181
    Clone ID Related Accession (Same CDS sequence) XM_002943148.4 , XM_002943148.5
    Accession Version XM_002943148.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4380bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002943148.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG AAGAGGAAAA TCCGTGTTCA
    ACAGGTCCTT CAAATCGTTC CAACTCAGTT TCCTCTTTGG ATCTGGAAGG GGAGTCTGTA
    TCTGAGCTAG GAGGAGGACC ATCCGGAAGC AATGGGGTGG AGGCTTTGCA GTTATTGGAA
    CATGAACAAG CTACCACACA GGATAATCTA GATGACAAAC TGCGCAAATT TGAAATTCGA
    GACATGATGG GATTGACGGA TGATAGAGAT ATATCAGAGA CAGTCAGTGA GACATGGAGC
    ACGGATGTGC TTGGAAGTGA TTTTGACCCC AACATTGATG AAGATCGACT ACAAGAAATT
    GCAGGTGCAG CAGCTGAGAA CATGCTGGGA AGCCTGCTGT GCCTTCCCGG CTCTGGCTCA
    ATACTGCTTG ATCCTTGTAC TGGATCCACT ATATCTGAGA CTACCAGTGA AGCATGGAGT
    GTTGAGGTGC TCCCTAGTGA TTCTGAAGCA CCAGATCTAA AGCAAGAGGA GCGGCTGCAG
    GAACTGGAGA GCTGTTCAGG TTTGGGAAGC ACATCAGATG ACACTGAAGT TAGAGAGGTC
    AGCTCCCGGC CCAGCACTCC AGGACTCAGT GTGGTGTCAG GTATCAGTGC TACCTCAGAG
    GACATCCCAA ACAAGATAGA GGACCTACGC TCAGAGTGCA GCTCTGACTT TGGAGGAAAG
    GATTCTGTTA CAAGTCCTGA GGCAGAAGAG TCTGTTCATG GACCTCACCA TATAACTGCA
    CCTCCAACCC AGACTGAATC CTTGTTGGCC ATGTTTGACC CACTAGCTCC AGCATCCAAT
    GAAGTGGTTC GACCTAAAGT ACACTATGCG AGACCCTCAC ATCCGCCTCC TGACCCGCCT
    GTACTGGAAG GAGCAAGTGG GGGAAATGAA GCTCGTCTTC CTATGTTCGG TTCTCATGTA
    TTTGTTCCCA TGGAGTCTGA AGCTTACAGG CAGAGACACT CATGTCCTGA AAGACTGGTG
    CGCAGTCGCA GCTCCGACAT CGCTTCTTCC ATACGGAGGC CAATGAGTGA CCCTGGCTGG
    GTGCGGCGTG GGGTGAATGA AGATAGGGAG CTGCCAACAG GAACTGCAAT TAATGCACTA
    ATTAATCCCC CCCAGGCTTC ATCCTCCTCA CCAAGCAAGG ACTCTTCCAG GGGAGAGCCA
    GAAGAAAAAA AAGACAGCGA TGATGAGCGA TCAGATAGGA ATAAGCCTTG GTGGAAGAAG
    CGGTTTGTGT CTGCAATGCC CAAAGCTCCT ATCCCATTTA GGAAGAAGGA AAAACACGAG
    AAGGAGAGGG AAGACTTGGT ACAAGACAGG TTTTCAGTAG CAGATGACTC AACATCTCAG
    CTAGGGCCAC ATTCCCAGGC AGCAGAAGAC ATCCTGGCTA AGTACAGGAA TGCTATTAAG
    AGGACAATCC CCAATGAAGG TTCCTCCGTG CCCTATGAAG GAGCAGATGC TGTTGGAGAT
    GGAGAAAGCC TTCATGATTC TCCTCGTGAT GAAGCTTTGC AGAACATGGC CTCAGATGAC
    CTGCTTGATT CTGCCAGCCA AGTAGCCCAC CCACAGGAGC CTGCATTCTC CTATAGGGAT
    GCTAAGAAAA AATTACGCCT GGCCCTGTGC TCTGCGGATT CAGTAGCGTT CCCTGTATTG
    TCTCATTCGA CACGGAATGG CCTGCCAGAT CACACAGACC CAGAAGACAA TGAGATTGTG
    TGCTTCCTTA AAGTCCAACT TGCAGAGGCC ATAAACCTGC AGGACAAAAA CCTGATGGCT
    CAGATCCAGG AAACTATGCG ATGTGTGAGC CGATTTGACA GCCGCTCCTG CAGCAAAATT
    TTGGCATCCA TTGCAGAGGA TTACCGGAAG AGGGCCTCAT ATATTGCATA TCTAACAAGA
    TGCCGACAGG GACTACAGAC CACCCAGGCT CACCTTGAGC GCCTCCTGCA GAGAGTGCTA
    CGAGATAAAG AGGTCTCCAC GCGCTACTTC ACCACTGTCT GTGTGAGGCT TCTACTTGAG
    AGCAAAGAGA AGGAAATCCA TGAATTTATC CAAGATTTTC AAAAGTTAAC TGCAGCTGAT
    GATAAAACAG CACAAGTTGA AGAGTTCTTG CAATCACTTT ACGGGGCTAT GGCACAAGAT
    GTGATTTGGC AAAATGCCAG TGAGGAGCAG CTGCAAGATG CACAGATTGC TATTGAGCGA
    AGCATTATGA ACCGCATCTT TAAGCTGGCC TTCTATCCCA ATCAAGATGC AGATATTCTG
    CGAGATCAGG TCCTGCATGA ACACATTAAA AGGTTGTCTA AAGTGGTGAC TGCCAATCAC
    AGGGCACTGC AGATACCTGA GGTGTATCTG AGGGAGGCTC CTTGGCCATC TGCCCAAACC
    GAAATCCGTA CCATCAGCGC ATACAAGACA CCCAGGGACA AAGTGCAGTG CATATTGCGC
    ATGTGCTCAA CAATCATGAA CCTTCTAAGC CTGGCCAATG AGTACTCTGT CCCAGGAGCA
    GATGATTTTG TTCCTGTGCT GGTTTTTGTC CTTATTAAGG CTAATCCCCC CTGCCTACTA
    TCAACAGTGC AATATATCAG CAACTTCTAT TCCAATCGGC TCACTGGCGA GGAGTCCTAC
    TGGTGGATGC AGTTCACAGC TGCTGTTGAG TTCATTAAAA CCATTGACGA TCGCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002943194.1
    CDS167..4546
    Translation

    Target ORF information:

    RefSeq Version XM_002943148.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002943148.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG AAGAGGAAAA TCCGTGTTCA
    ACAGGTCCTT CAAATCGTTC CAACTCAGTT TCCTCTTTGG ATCTGGAAGG GGAGTCTGTA
    TCTGAGCTAG GAGGAGGACC ATCCGGAAGC AATGGGGTGG AGGCTTTGCA GTTATTGGAA
    CATGAACAAG CTACCACACA GGATAATCTA GATGACAAAC TGCGCAAATT TGAAATTCGA
    GACATGATGG GATTGACGGA TGATAGAGAT ATATCAGAGA CAGTCAGTGA GACATGGAGC
    ACGGATGTGC TTGGAAGTGA TTTTGACCCC AACATTGATG AAGATCGACT ACAAGAAATT
    GCAGGTGCAG CAGCTGAGAA CATGCTGGGA AGCCTGCTGT GCCTTCCCGG CTCTGGCTCA
    ATACTGCTTG ATCCTTGTAC TGGATCCACT ATATCTGAGA CTACCAGTGA AGCATGGAGT
    GTTGAGGTGC TCCCTAGTGA TTCTGAAGCA CCAGATCTAA AGCAAGAGGA GCGGCTGCAG
    GAACTGGAGA GCTGTTCAGG TTTGGGAAGC ACATCAGATG ACACTGAAGT TAGAGAGGTC
    AGCTCCCGGC CCAGCACTCC AGGACTCAGT GTGGTGTCAG GTATCAGTGC TACCTCAGAG
    GACATCCCAA ACAAGATAGA GGACCTACGC TCAGAGTGCA GCTCTGACTT TGGAGGAAAG
    GATTCTGTTA CAAGTCCTGA GGCAGAAGAG TCTGTTCATG GACCTCACCA TATAACTGCA
    CCTCCAACCC AGACTGAATC CTTGTTGGCC ATGTTTGACC CACTAGCTCC AGCATCCAAT
    GAAGTGGTTC GACCTAAAGT ACACTATGCG AGACCCTCAC ATCCGCCTCC TGACCCGCCT
    GTACTGGAAG GAGCAAGTGG GGGAAATGAA GCTCGTCTTC CTATGTTCGG TTCTCATGTA
    TTTGTTCCCA TGGAGTCTGA AGCTTACAGG CAGAGACACT CATGTCCTGA AAGACTGGTG
    CGCAGTCGCA GCTCCGACAT CGCTTCTTCC ATACGGAGGC CAATGAGTGA CCCTGGCTGG
    GTGCGGCGTG GGGTGAATGA AGATAGGGAG CTGCCAACAG GAACTGCAAT TAATGCACTA
    ATTAATCCCC CCCAGGCTTC ATCCTCCTCA CCAAGCAAGG ACTCTTCCAG GGGAGAGCCA
    GAAGAAAAAA AAGACAGCGA TGATGAGCGA TCAGATAGGA ATAAGCCTTG GTGGAAGAAG
    CGGTTTGTGT CTGCAATGCC CAAAGCTCCT ATCCCATTTA GGAAGAAGGA AAAACACGAG
    AAGGAGAGGG AAGACTTGGT ACAAGACAGG TTTTCAGTAG CAGATGACTC AACATCTCAG
    CTAGGGCCAC ATTCCCAGGC AGCAGAAGAC ATCCTGGCTA AGTACAGGAA TGCTATTAAG
    AGGACAATCC CCAATGAAGG TTCCTCCGTG CCCTATGAAG GAGCAGATGC TGTTGGAGAT
    GGAGAAAGCC TTCATGATTC TCCTCGTGAT GAAGCTTTGC AGAACATGGC CTCAGATGAC
    CTGCTTGATT CTGCCAGCCA AGTAGCCCAC CCACAGGAGC CTGCATTCTC CTATAGGGAT
    GCTAAGAAAA AATTACGCCT GGCCCTGTGC TCTGCGGATT CAGTAGCGTT CCCTGTATTG
    TCTCATTCGA CACGGAATGG CCTGCCAGAT CACACAGACC CAGAAGACAA TGAGATTGTG
    TGCTTCCTTA AAGTCCAACT TGCAGAGGCC ATAAACCTGC AGGACAAAAA CCTGATGGCT
    CAGATCCAGG AAACTATGCG ATGTGTGAGC CGATTTGACA GCCGCTCCTG CAGCAAAATT
    TTGGCATCCA TTGCAGAGGA TTACCGGAAG AGGGCCTCAT ATATTGCATA TCTAACAAGA
    TGCCGACAGG GACTACAGAC CACCCAGGCT CACCTTGAGC GCCTCCTGCA GAGAGTGCTA
    CGAGATAAAG AGGTCTCCAC GCGCTACTTC ACCACTGTCT GTGTGAGGCT TCTACTTGAG
    AGCAAAGAGA AGGAAATCCA TGAATTTATC CAAGATTTTC AAAAGTTAAC TGCAGCTGAT
    GATAAAACAG CACAAGTTGA AGAGTTCTTG CAATCACTTT ACGGGGCTAT GGCACAAGAT
    GTGATTTGGC AAAATGCCAG TGAGGAGCAG CTGCAAGATG CACAGATTGC TATTGAGCGA
    AGCATTATGA ACCGCATCTT TAAGCTGGCC TTCTATCCCA ATCAAGATGC AGATATTCTG
    CGAGATCAGG TCCTGCATGA ACACATTAAA AGGTTGTCTA AAGTGGTGAC TGCCAATCAC
    AGGGCACTGC AGATACCTGA GGTGTATCTG AGGGAGGCTC CTTGGCCATC TGCCCAAACC
    GAAATCCGTA CCATCAGCGC ATACAAGACA CCCAGGGACA AAGTGCAGTG CATATTGCGC
    ATGTGCTCAA CAATCATGAA CCTTCTAAGC CTGGCCAATG AGTACTCTGT CCCAGGAGCA
    GATGATTTTG TTCCTGTGCT GGTTTTTGTC CTTATTAAGG CTAATCCCCC CTGCCTACTA
    TCAACAGTGC AATATATCAG CAACTTCTAT TCCAATCGGC TCACTGGCGA GGAGTCCTAC
    TGGTGGATGC AGTTCACAGC TGCTGTTGAG TTCATTAAAA CCATTGACGA TCGCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14182
    Clone ID Related Accession (Same CDS sequence) XM_004916739.3 , XM_004916739.4
    Accession Version XM_004916739.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916739.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG AAGAGGAAAA TCCGTGTTCA
    ACAGGTCCTT CAAATCGTTC CAACTCAGTT TCCTCTTTGG ATCTGGAAGG GGAGTCTGTA
    TCTGAGCTAG GAGGAGGACC ATCCGGAAGC AATGGGGTGG AGGCTTTGCA GTTATTGGAA
    CATGAACAAG CTACCACACA GGATAATCTA GATGACAAAC TGCGCAAATT TGAAATTCGA
    GACATGATGG GATTGACGGA TGATAGAGAT ATATCAGAGA CAGTCAGTGA GACATGGAGC
    ACGGATGTGC TTGGAAGTGA TTTTGACCCC AACATTGATG AAGATCGACT ACAAGAAATT
    GCAGGTGCAG CAGCTGAGAA CATGCTGGGA AGCCTGCTGT GCCTTCCCGG CTCTGGCTCA
    ATACTGCTTG ATCCTTGTAC TGGATCCACT ATATCTGAGA CTACCAGTGA AGCATGGAGT
    GTTGAGGTGC TCCCTAGTGA TTCTGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTA TCAGTGCTAC CTCAGAGGAC
    ATCCCAAACA AGATAGAGGA CCTACGCTCA GAGTGCAGCT CTGACTTTGG AGGAAAGGAT
    TCTGTTACAA GTCCTGAGGC AGAAGAGTCT GTTCATGGAC CTCACCATAT AACTGCACCT
    CCAACCCAGA CTGAATCCTT GTTGGCCATG TTTGACCCAC TAGCTCCAGC ATCCAATGAA
    GTGGTTCGAC CTAAAGTACA CTATGCGAGA CCCTCACATC CGCCTCCTGA CCCGCCTGTA
    CTGGAAGGAG CAAGTGGGGG AAATGAAGCT CGTCTTCCTA TGTTCGGTTC TCATGTATTT
    GTTCCCATGG AGTCTGAAGC TTACAGGCAG AGACACTCAT GTCCTGAAAG ACTGGTGCGC
    AGTCGCAGCT CCGACATCGC TTCTTCCATA CGGAGGCCAA TGAGTGACCC TGGCTGGGTG
    CGGCGTGGGG TGAATGAAGA TAGGGAGCTG CCAACAGGAA CTGCAATTAA TGCACTAATT
    AATCCCCCCC AGGCTTCATC CTCCTCACCA AGCAAGGACT CTTCCAGGGG AGAGCCAGAA
    GAAAAAAAAG ACAGCGATGA TGAGCGATCA GATAGGAATA AGCCTTGGTG GAAGAAGCGG
    TTTGTGTCTG CAATGCCCAA AGCTCCTATC CCATTTAGGA AGAAGGAAAA ACACGAGAAG
    GAGAGGGAAG ACTTGGTACA AGACAGGTTT TCAGTAGCAG ATGACTCAAC ATCTCAGCTA
    GGGCCACATT CCCAGGCAGC AGAAGACATC CTGGCTAAGT ACAGGAATGC TATTAAGAGG
    ACAATCCCCA ATGAAGGTTC CTCCGTGCCC TATGAAGGAG CAGATGCTGT TGGAGATGGA
    GAAAGCCTTC ATGATTCTCC TCGTGATGAA GCTTTGCAGA ACATGGCCTC AGATGACCTG
    CTTGATTCTG CCAGCCAAGT AGCCCACCCA CAGGAGCCTG CATTCTCCTA TAGGGATGCT
    AAGAAAAAAT TACGCCTGGC CCTGTGCTCT GCGGATTCAG TAGCGTTCCC TGTATTGTCT
    CATTCGACAC GGAATGGCCT GCCAGATCAC ACAGACCCAG AAGACAATGA GATTGTGTGC
    TTCCTTAAAG TCCAACTTGC AGAGGCCATA AACCTGCAGG ACAAAAACCT GATGGCTCAG
    ATCCAGGAAA CTATGCGATG TGTGAGCCGA TTTGACAGCC GCTCCTGCAG CAAAATTTTG
    GCATCCATTG CAGAGGATTA CCGGAAGAGG GCCTCATATA TTGCATATCT AACAAGATGC
    CGACAGGGAC TACAGACCAC CCAGGCTCAC CTTGAGCGCC TCCTGCAGAG AGTGCTACGA
    GATAAAGAGG TCTCCACGCG CTACTTCACC ACTGTCTGTG TGAGGCTTCT ACTTGAGAGC
    AAAGAGAAGG AAATCCATGA ATTTATCCAA GATTTTCAAA AGTTAACTGC AGCTGATGAT
    AAAACAGCAC AAGTTGAAGA GTTCTTGCAA TCACTTTACG GGGCTATGGC ACAAGATGTG
    ATTTGGCAAA ATGCCAGTGA GGAGCAGCTG CAAGATGCAC AGATTGCTAT TGAGCGAAGC
    ATTATGAACC GCATCTTTAA GCTGGCCTTC TATCCCAATC AAGATGCAGA TATTCTGCGA
    GATCAGGTCC TGCATGAACA CATTAAAAGG TTGTCTAAAG TGGTGACTGC CAATCACAGG
    GCACTGCAGA TACCTGAGGT GTATCTGAGG GAGGCTCCTT GGCCATCTGC CCAAACCGAA
    ATCCGTACCA TCAGCGCATA CAAGACACCC AGGGACAAAG TGCAGTGCAT ATTGCGCATG
    TGCTCAACAA TCATGAACCT TCTAAGCCTG GCCAATGAGT ACTCTGTCCC AGGAGCAGAT
    GATTTTGTTC CTGTGCTGGT TTTTGTCCTT ATTAAGGCTA ATCCCCCCTG CCTACTATCA
    ACAGTGCAAT ATATCAGCAA CTTCTATTCC AATCGGCTCA CTGGCGAGGA GTCCTACTGG
    TGGATGCAGT TCACAGCTGC TGTTGAGTTC ATTAAAACCA TTGACGATCG CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916796.1
    CDS168..4544
    Translation

    Target ORF information:

    RefSeq Version XM_004916739.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916739.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG AAGAGGAAAA TCCGTGTTCA
    ACAGGTCCTT CAAATCGTTC CAACTCAGTT TCCTCTTTGG ATCTGGAAGG GGAGTCTGTA
    TCTGAGCTAG GAGGAGGACC ATCCGGAAGC AATGGGGTGG AGGCTTTGCA GTTATTGGAA
    CATGAACAAG CTACCACACA GGATAATCTA GATGACAAAC TGCGCAAATT TGAAATTCGA
    GACATGATGG GATTGACGGA TGATAGAGAT ATATCAGAGA CAGTCAGTGA GACATGGAGC
    ACGGATGTGC TTGGAAGTGA TTTTGACCCC AACATTGATG AAGATCGACT ACAAGAAATT
    GCAGGTGCAG CAGCTGAGAA CATGCTGGGA AGCCTGCTGT GCCTTCCCGG CTCTGGCTCA
    ATACTGCTTG ATCCTTGTAC TGGATCCACT ATATCTGAGA CTACCAGTGA AGCATGGAGT
    GTTGAGGTGC TCCCTAGTGA TTCTGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTA TCAGTGCTAC CTCAGAGGAC
    ATCCCAAACA AGATAGAGGA CCTACGCTCA GAGTGCAGCT CTGACTTTGG AGGAAAGGAT
    TCTGTTACAA GTCCTGAGGC AGAAGAGTCT GTTCATGGAC CTCACCATAT AACTGCACCT
    CCAACCCAGA CTGAATCCTT GTTGGCCATG TTTGACCCAC TAGCTCCAGC ATCCAATGAA
    GTGGTTCGAC CTAAAGTACA CTATGCGAGA CCCTCACATC CGCCTCCTGA CCCGCCTGTA
    CTGGAAGGAG CAAGTGGGGG AAATGAAGCT CGTCTTCCTA TGTTCGGTTC TCATGTATTT
    GTTCCCATGG AGTCTGAAGC TTACAGGCAG AGACACTCAT GTCCTGAAAG ACTGGTGCGC
    AGTCGCAGCT CCGACATCGC TTCTTCCATA CGGAGGCCAA TGAGTGACCC TGGCTGGGTG
    CGGCGTGGGG TGAATGAAGA TAGGGAGCTG CCAACAGGAA CTGCAATTAA TGCACTAATT
    AATCCCCCCC AGGCTTCATC CTCCTCACCA AGCAAGGACT CTTCCAGGGG AGAGCCAGAA
    GAAAAAAAAG ACAGCGATGA TGAGCGATCA GATAGGAATA AGCCTTGGTG GAAGAAGCGG
    TTTGTGTCTG CAATGCCCAA AGCTCCTATC CCATTTAGGA AGAAGGAAAA ACACGAGAAG
    GAGAGGGAAG ACTTGGTACA AGACAGGTTT TCAGTAGCAG ATGACTCAAC ATCTCAGCTA
    GGGCCACATT CCCAGGCAGC AGAAGACATC CTGGCTAAGT ACAGGAATGC TATTAAGAGG
    ACAATCCCCA ATGAAGGTTC CTCCGTGCCC TATGAAGGAG CAGATGCTGT TGGAGATGGA
    GAAAGCCTTC ATGATTCTCC TCGTGATGAA GCTTTGCAGA ACATGGCCTC AGATGACCTG
    CTTGATTCTG CCAGCCAAGT AGCCCACCCA CAGGAGCCTG CATTCTCCTA TAGGGATGCT
    AAGAAAAAAT TACGCCTGGC CCTGTGCTCT GCGGATTCAG TAGCGTTCCC TGTATTGTCT
    CATTCGACAC GGAATGGCCT GCCAGATCAC ACAGACCCAG AAGACAATGA GATTGTGTGC
    TTCCTTAAAG TCCAACTTGC AGAGGCCATA AACCTGCAGG ACAAAAACCT GATGGCTCAG
    ATCCAGGAAA CTATGCGATG TGTGAGCCGA TTTGACAGCC GCTCCTGCAG CAAAATTTTG
    GCATCCATTG CAGAGGATTA CCGGAAGAGG GCCTCATATA TTGCATATCT AACAAGATGC
    CGACAGGGAC TACAGACCAC CCAGGCTCAC CTTGAGCGCC TCCTGCAGAG AGTGCTACGA
    GATAAAGAGG TCTCCACGCG CTACTTCACC ACTGTCTGTG TGAGGCTTCT ACTTGAGAGC
    AAAGAGAAGG AAATCCATGA ATTTATCCAA GATTTTCAAA AGTTAACTGC AGCTGATGAT
    AAAACAGCAC AAGTTGAAGA GTTCTTGCAA TCACTTTACG GGGCTATGGC ACAAGATGTG
    ATTTGGCAAA ATGCCAGTGA GGAGCAGCTG CAAGATGCAC AGATTGCTAT TGAGCGAAGC
    ATTATGAACC GCATCTTTAA GCTGGCCTTC TATCCCAATC AAGATGCAGA TATTCTGCGA
    GATCAGGTCC TGCATGAACA CATTAAAAGG TTGTCTAAAG TGGTGACTGC CAATCACAGG
    GCACTGCAGA TACCTGAGGT GTATCTGAGG GAGGCTCCTT GGCCATCTGC CCAAACCGAA
    ATCCGTACCA TCAGCGCATA CAAGACACCC AGGGACAAAG TGCAGTGCAT ATTGCGCATG
    TGCTCAACAA TCATGAACCT TCTAAGCCTG GCCAATGAGT ACTCTGTCCC AGGAGCAGAT
    GATTTTGTTC CTGTGCTGGT TTTTGTCCTT ATTAAGGCTA ATCCCCCCTG CCTACTATCA
    ACAGTGCAAT ATATCAGCAA CTTCTATTCC AATCGGCTCA CTGGCGAGGA GTCCTACTGG
    TGGATGCAGT TCACAGCTGC TGTTGAGTTC ATTAAAACCA TTGACGATCG CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14183
    Clone ID Related Accession (Same CDS sequence) XM_004916740.3 , XM_004916740.4
    Accession Version XM_004916740.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916740.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG GTCCTTCAAA TCGTTCCAAC
    TCAGTTTCCT CTTTGGATCT GGAAGGGGAG TCTGTATCTG AGCTAGGAGG AGGACCATCC
    GGAAGCAATG GGGTGGAGGC TTTGCAGTTA TTGGAACATG AACAAGCTAC CACACAGGAT
    AATCTAGATG ACAAACTGCG CAAATTTGAA ATTCGAGACA TGATGGGATT GACGGATGAT
    AGAGATATAT CAGAGACAGT CAGTGAGACA TGGAGCACGG ATGTGCTTGG AAGTGATTTT
    GACCCCAACA TTGATGAAGA TCGACTACAA GAAATTGCAG GTGCAGCAGC TGAGAACATG
    CTGGGAAGCC TGCTGTGCCT TCCCGGCTCT GGCTCAATAC TGCTTGATCC TTGTACTGGA
    TCCACTATAT CTGAGACTAC CAGTGAAGCA TGGAGTGTTG AGGTGCTCCC TAGTGATTCT
    GAAGCACCAG ATCTAAAGCA AGAGGAGCGG CTGCAGGAAC TGGAGAGCTG TTCAGGTTTG
    GGAAGCACAT CAGATGACAC TGAAGTTAGA GAGGTCAGCT CCCGGCCCAG CACTCCAGGA
    CTCAGTGTGG TGTCAGGTAT CAGTGCTACC TCAGAGGACA TCCCAAACAA GATAGAGGAC
    CTACGCTCAG AGTGCAGCTC TGACTTTGGA GGAAAGGATT CTGTTACAAG TCCTGAGGCA
    GAAGAGTCTG TTCATGGACC TCACCATATA ACTGCACCTC CAACCCAGAC TGAATCCTTG
    TTGGCCATGT TTGACCCACT AGCTCCAGCA TCCAATGAAG TGGTTCGACC TAAAGTACAC
    TATGCGAGAC CCTCACATCC GCCTCCTGAC CCGCCTGTAC TGGAAGGAGC AAGTGGGGGA
    AATGAAGCTC GTCTTCCTAT GTTCGGTTCT CATGTATTTG TTCCCATGGA GTCTGAAGCT
    TACAGGCAGA GACACTCATG TCCTGAAAGA CTGGTGCGCA GTCGCAGCTC CGACATCGCT
    TCTTCCATAC GGAGGCCAAT GAGTGACCCT GGCTGGGTGC GGCGTGGGGT GAATGAAGAT
    AGGGAGCTGC CAACAGGAAC TGCAATTAAT GCACTAATTA ATCCCCCCCA GGCTTCATCC
    TCCTCACCAA GCAAGGACTC TTCCAGGGGA GAGCCAGAAG AAAAAAAAGA CAGCGATGAT
    GAGCGATCAG ATAGGAATAA GCCTTGGTGG AAGAAGCGGT TTGTGTCTGC AATGCCCAAA
    GCTCCTATCC CATTTAGGAA GAAGGAAAAA CACGAGAAGG AGAGGGAAGA CTTGGTACAA
    GACAGGTTTT CAGTAGCAGA TGACTCAACA TCTCAGCTAG GGCCACATTC CCAGGCAGCA
    GAAGACATCC TGGCTAAGTA CAGGAATGCT ATTAAGAGGA CAATCCCCAA TGAAGGTTCC
    TCCGTGCCCT ATGAAGGAGC AGATGCTGTT GGAGATGGAG AAAGCCTTCA TGATTCTCCT
    CGTGATGAAG CTTTGCAGAA CATGGCCTCA GATGACCTGC TTGATTCTGC CAGCCAAGTA
    GCCCACCCAC AGGAGCCTGC ATTCTCCTAT AGGGATGCTA AGAAAAAATT ACGCCTGGCC
    CTGTGCTCTG CGGATTCAGT AGCGTTCCCT GTATTGTCTC ATTCGACACG GAATGGCCTG
    CCAGATCACA CAGACCCAGA AGACAATGAG ATTGTGTGCT TCCTTAAAGT CCAACTTGCA
    GAGGCCATAA ACCTGCAGGA CAAAAACCTG ATGGCTCAGA TCCAGGAAAC TATGCGATGT
    GTGAGCCGAT TTGACAGCCG CTCCTGCAGC AAAATTTTGG CATCCATTGC AGAGGATTAC
    CGGAAGAGGG CCTCATATAT TGCATATCTA ACAAGATGCC GACAGGGACT ACAGACCACC
    CAGGCTCACC TTGAGCGCCT CCTGCAGAGA GTGCTACGAG ATAAAGAGGT CTCCACGCGC
    TACTTCACCA CTGTCTGTGT GAGGCTTCTA CTTGAGAGCA AAGAGAAGGA AATCCATGAA
    TTTATCCAAG ATTTTCAAAA GTTAACTGCA GCTGATGATA AAACAGCACA AGTTGAAGAG
    TTCTTGCAAT CACTTTACGG GGCTATGGCA CAAGATGTGA TTTGGCAAAA TGCCAGTGAG
    GAGCAGCTGC AAGATGCACA GATTGCTATT GAGCGAAGCA TTATGAACCG CATCTTTAAG
    CTGGCCTTCT ATCCCAATCA AGATGCAGAT ATTCTGCGAG ATCAGGTCCT GCATGAACAC
    ATTAAAAGGT TGTCTAAAGT GGTGACTGCC AATCACAGGG CACTGCAGAT ACCTGAGGTG
    TATCTGAGGG AGGCTCCTTG GCCATCTGCC CAAACCGAAA TCCGTACCAT CAGCGCATAC
    AAGACACCCA GGGACAAAGT GCAGTGCATA TTGCGCATGT GCTCAACAAT CATGAACCTT
    CTAAGCCTGG CCAATGAGTA CTCTGTCCCA GGAGCAGATG ATTTTGTTCC TGTGCTGGTT
    TTTGTCCTTA TTAAGGCTAA TCCCCCCTGC CTACTATCAA CAGTGCAATA TATCAGCAAC
    TTCTATTCCA ATCGGCTCAC TGGCGAGGAG TCCTACTGGT GGATGCAGTT CACAGCTGCT
    GTTGAGTTCA TTAAAACCAT TGACGATCGC AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916797.1
    CDS167..4522
    Translation

    Target ORF information:

    RefSeq Version XM_004916740.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916740.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG GTCCTTCAAA TCGTTCCAAC
    TCAGTTTCCT CTTTGGATCT GGAAGGGGAG TCTGTATCTG AGCTAGGAGG AGGACCATCC
    GGAAGCAATG GGGTGGAGGC TTTGCAGTTA TTGGAACATG AACAAGCTAC CACACAGGAT
    AATCTAGATG ACAAACTGCG CAAATTTGAA ATTCGAGACA TGATGGGATT GACGGATGAT
    AGAGATATAT CAGAGACAGT CAGTGAGACA TGGAGCACGG ATGTGCTTGG AAGTGATTTT
    GACCCCAACA TTGATGAAGA TCGACTACAA GAAATTGCAG GTGCAGCAGC TGAGAACATG
    CTGGGAAGCC TGCTGTGCCT TCCCGGCTCT GGCTCAATAC TGCTTGATCC TTGTACTGGA
    TCCACTATAT CTGAGACTAC CAGTGAAGCA TGGAGTGTTG AGGTGCTCCC TAGTGATTCT
    GAAGCACCAG ATCTAAAGCA AGAGGAGCGG CTGCAGGAAC TGGAGAGCTG TTCAGGTTTG
    GGAAGCACAT CAGATGACAC TGAAGTTAGA GAGGTCAGCT CCCGGCCCAG CACTCCAGGA
    CTCAGTGTGG TGTCAGGTAT CAGTGCTACC TCAGAGGACA TCCCAAACAA GATAGAGGAC
    CTACGCTCAG AGTGCAGCTC TGACTTTGGA GGAAAGGATT CTGTTACAAG TCCTGAGGCA
    GAAGAGTCTG TTCATGGACC TCACCATATA ACTGCACCTC CAACCCAGAC TGAATCCTTG
    TTGGCCATGT TTGACCCACT AGCTCCAGCA TCCAATGAAG TGGTTCGACC TAAAGTACAC
    TATGCGAGAC CCTCACATCC GCCTCCTGAC CCGCCTGTAC TGGAAGGAGC AAGTGGGGGA
    AATGAAGCTC GTCTTCCTAT GTTCGGTTCT CATGTATTTG TTCCCATGGA GTCTGAAGCT
    TACAGGCAGA GACACTCATG TCCTGAAAGA CTGGTGCGCA GTCGCAGCTC CGACATCGCT
    TCTTCCATAC GGAGGCCAAT GAGTGACCCT GGCTGGGTGC GGCGTGGGGT GAATGAAGAT
    AGGGAGCTGC CAACAGGAAC TGCAATTAAT GCACTAATTA ATCCCCCCCA GGCTTCATCC
    TCCTCACCAA GCAAGGACTC TTCCAGGGGA GAGCCAGAAG AAAAAAAAGA CAGCGATGAT
    GAGCGATCAG ATAGGAATAA GCCTTGGTGG AAGAAGCGGT TTGTGTCTGC AATGCCCAAA
    GCTCCTATCC CATTTAGGAA GAAGGAAAAA CACGAGAAGG AGAGGGAAGA CTTGGTACAA
    GACAGGTTTT CAGTAGCAGA TGACTCAACA TCTCAGCTAG GGCCACATTC CCAGGCAGCA
    GAAGACATCC TGGCTAAGTA CAGGAATGCT ATTAAGAGGA CAATCCCCAA TGAAGGTTCC
    TCCGTGCCCT ATGAAGGAGC AGATGCTGTT GGAGATGGAG AAAGCCTTCA TGATTCTCCT
    CGTGATGAAG CTTTGCAGAA CATGGCCTCA GATGACCTGC TTGATTCTGC CAGCCAAGTA
    GCCCACCCAC AGGAGCCTGC ATTCTCCTAT AGGGATGCTA AGAAAAAATT ACGCCTGGCC
    CTGTGCTCTG CGGATTCAGT AGCGTTCCCT GTATTGTCTC ATTCGACACG GAATGGCCTG
    CCAGATCACA CAGACCCAGA AGACAATGAG ATTGTGTGCT TCCTTAAAGT CCAACTTGCA
    GAGGCCATAA ACCTGCAGGA CAAAAACCTG ATGGCTCAGA TCCAGGAAAC TATGCGATGT
    GTGAGCCGAT TTGACAGCCG CTCCTGCAGC AAAATTTTGG CATCCATTGC AGAGGATTAC
    CGGAAGAGGG CCTCATATAT TGCATATCTA ACAAGATGCC GACAGGGACT ACAGACCACC
    CAGGCTCACC TTGAGCGCCT CCTGCAGAGA GTGCTACGAG ATAAAGAGGT CTCCACGCGC
    TACTTCACCA CTGTCTGTGT GAGGCTTCTA CTTGAGAGCA AAGAGAAGGA AATCCATGAA
    TTTATCCAAG ATTTTCAAAA GTTAACTGCA GCTGATGATA AAACAGCACA AGTTGAAGAG
    TTCTTGCAAT CACTTTACGG GGCTATGGCA CAAGATGTGA TTTGGCAAAA TGCCAGTGAG
    GAGCAGCTGC AAGATGCACA GATTGCTATT GAGCGAAGCA TTATGAACCG CATCTTTAAG
    CTGGCCTTCT ATCCCAATCA AGATGCAGAT ATTCTGCGAG ATCAGGTCCT GCATGAACAC
    ATTAAAAGGT TGTCTAAAGT GGTGACTGCC AATCACAGGG CACTGCAGAT ACCTGAGGTG
    TATCTGAGGG AGGCTCCTTG GCCATCTGCC CAAACCGAAA TCCGTACCAT CAGCGCATAC
    AAGACACCCA GGGACAAAGT GCAGTGCATA TTGCGCATGT GCTCAACAAT CATGAACCTT
    CTAAGCCTGG CCAATGAGTA CTCTGTCCCA GGAGCAGATG ATTTTGTTCC TGTGCTGGTT
    TTTGTCCTTA TTAAGGCTAA TCCCCCCTGC CTACTATCAA CAGTGCAATA TATCAGCAAC
    TTCTATTCCA ATCGGCTCAC TGGCGAGGAG TCCTACTGGT GGATGCAGTT CACAGCTGCT
    GTTGAGTTCA TTAAAACCAT TGACGATCGC AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31122
    Clone ID Related Accession (Same CDS sequence) XM_012969064.2 , XM_012969064.3
    Accession Version XM_012969064.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969064.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GAAGAGGAAA ATCCGTGTTC AACAGGTCCT TCAAATCGTT CCAACTCAGT TTCCTCTTTG
    GATCTGGAAG GGGAGTCTGT ATCTGAGCTA GGAGGAGGAC CATCCGGAAG CAATGGGGTG
    GAGGCTTTGC AGTTATTGGA ACATGAACAA GCTACCACAC AGGATAATCT AGATGACAAA
    CTGCGCAAAT TTGAAATTCG AGACATGATG GGATTGACGG ATGATAGAGA TATATCAGAG
    ACAGTCAGTG AGACATGGAG CACGGATGTG CTTGGAAGTG ATTTTGACCC CAACATTGAT
    GAAGATCGAC TACAAGAAAT TGCAGGTGCA GCAGCTGAGA ACATGCTGGG AAGCCTGCTG
    TGCCTTCCCG GCTCTGGCTC AATACTGCTT GATCCTTGTA CTGGATCCAC TATATCTGAG
    ACTACCAGTG AAGCATGGAG TGTTGAGGTG CTCCCTAGTG ATTCTGAAGC ACCAGATCTA
    AAGCAAGAGG AGCGGCTGCA GGAACTGGAG AGCTGTTCAG GTTTGGGAAG CACATCAGAT
    GACACTGAAG TTAGAGAGGT CAGCTCCCGG CCCAGCACTC CAGGACTCAG TGTGGTGTCA
    GGTGACTTTC CCAAACCTTT CTATTCATTG CCGGGTATCA GTGCTACCTC AGAGGACATC
    CCAAACAAGA TAGAGGACCT ACGCTCAGAG TGCAGCTCTG ACTTTGGAGG AAAGGATTCT
    GTTACAAGTC CTGAGGCAGA AGAGTCTGTT CATGGACCTC ACCATATAAC TGCACCTCCA
    ACCCAGACTG AATCCTTGTT GGCCATGTTT GACCCACTAG CTCCAGCATC CAATGAAGTG
    GTTCGACCTA AAGTACACTA TGCGAGACCC TCACATCCGC CTCCTGACCC GCCTGTACTG
    GAAGGAGCAA GTGGGGGAAA TGAAGCTCGT CTTCCTATGT TCGGTTCTCA TGTATTTGTT
    CCCATGGAGT CTGAAGCTTA CAGGCAGAGA CACTCATGTC CTGAAAGACT GGTGCGCAGT
    CGCAGCTCCG ACATCGCTTC TTCCATACGG AGGCCAATGA GTGACCCTGG CTGGGTGCGG
    CGTGGGGTGA ATGAAGATAG GGAGCTGCCA ACAGGAACTG CAATTAATGC ACTAATTAAT
    CCCCCCCAGG CTTCATCCTC CTCACCAAGC AAGGACTCTT CCAGGGGAGA GCCAGAAGAA
    AAAAAAGACA GCGATGATGA GCGATCAGAT AGGAATAAGC CTTGGTGGAA GAAGCGGTTT
    GTGTCTGCAA TGCCCAAAGC TCCTATCCCA TTTAGGAAGA AGGAAAAACA CGAGAAGGAG
    AGGGAAGACT TGGTACAAGA CAGGTTTTCA GTAGCAGATG ACTCAACATC TCAGCTAGGG
    CCACATTCCC AGGCAGCAGA AGACATCCTG GCTAAGTACA GGAATGCTAT TAAGAGGACA
    ATCCCCAATG AAGGTTCCTC CGTGCCCTAT GAAGGAGCAG ATGCTGTTGG AGATGGAGAA
    AGCCTTCATG ATTCTCCTCG TGATGAAGCT TTGCAGAACA TGGCCTCAGA TGACCTGCTT
    GATTCTGCCA GCCAAGTAGC CCACCCACAG GAGCCTGCAT TCTCCTATAG GGATGCTAAG
    AAAAAATTAC GCCTGGCCCT GTGCTCTGCG GATTCAGTAG CGTTCCCTGT ATTGTCTCAT
    TCGACACGGA ATGGCCTGCC AGATCACACA GACCCAGAAG ACAATGAGAT TGTGTGCTTC
    CTTAAAGTCC AACTTGCAGA GGCCATAAAC CTGCAGGACA AAAACCTGAT GGCTCAGATC
    CAGGAAACTA TGCGATGTGT GAGCCGATTT GACAGCCGCT CCTGCAGCAA AATTTTGGCA
    TCCATTGCAG AGGATTACCG GAAGAGGGCC TCATATATTG CATATCTAAC AAGATGCCGA
    CAGGGACTAC AGACCACCCA GGCTCACCTT GAGCGCCTCC TGCAGAGAGT GCTACGAGAT
    AAAGAGGTCT CCACGCGCTA CTTCACCACT GTCTGTGTGA GGCTTCTACT TGAGAGCAAA
    GAGAAGGAAA TCCATGAATT TATCCAAGAT TTTCAAAAGT TAACTGCAGC TGATGATAAA
    ACAGCACAAG TTGAAGAGTT CTTGCAATCA CTTTACGGGG CTATGGCACA AGATGTGATT
    TGGCAAAATG CCAGTGAGGA GCAGCTGCAA GATGCACAGA TTGCTATTGA GCGAAGCATT
    ATGAACCGCA TCTTTAAGCT GGCCTTCTAT CCCAATCAAG ATGCAGATAT TCTGCGAGAT
    CAGGTCCTGC ATGAACACAT TAAAAGGTTG TCTAAAGTGG TGACTGCCAA TCACAGGGCA
    CTGCAGATAC CTGAGGTGTA TCTGAGGGAG GCTCCTTGGC CATCTGCCCA AACCGAAATC
    CGTACCATCA GCGCATACAA GACACCCAGG GACAAAGTGC AGTGCATATT GCGCATGTGC
    TCAACAATCA TGAACCTTCT AAGCCTGGCC AATGAGTACT CTGTCCCAGG AGCAGATGAT
    TTTGTTCCTG TGCTGGTTTT TGTCCTTATT AAGGCTAATC CCCCCTGCCT ACTATCAACA
    GTGCAATATA TCAGCAACTT CTATTCCAAT CGGCTCACTG GCGAGGAGTC CTACTGGTGG
    ATGCAGTTCA CAGCTGCTGT TGAGTTCATT AAAACCATTG ACGATCGCAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824518.1
    CDS170..4663
    Translation

    Target ORF information:

    RefSeq Version XM_012969064.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969064.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GAAGAGGAAA ATCCGTGTTC AACAGGTCCT TCAAATCGTT CCAACTCAGT TTCCTCTTTG
    GATCTGGAAG GGGAGTCTGT ATCTGAGCTA GGAGGAGGAC CATCCGGAAG CAATGGGGTG
    GAGGCTTTGC AGTTATTGGA ACATGAACAA GCTACCACAC AGGATAATCT AGATGACAAA
    CTGCGCAAAT TTGAAATTCG AGACATGATG GGATTGACGG ATGATAGAGA TATATCAGAG
    ACAGTCAGTG AGACATGGAG CACGGATGTG CTTGGAAGTG ATTTTGACCC CAACATTGAT
    GAAGATCGAC TACAAGAAAT TGCAGGTGCA GCAGCTGAGA ACATGCTGGG AAGCCTGCTG
    TGCCTTCCCG GCTCTGGCTC AATACTGCTT GATCCTTGTA CTGGATCCAC TATATCTGAG
    ACTACCAGTG AAGCATGGAG TGTTGAGGTG CTCCCTAGTG ATTCTGAAGC ACCAGATCTA
    AAGCAAGAGG AGCGGCTGCA GGAACTGGAG AGCTGTTCAG GTTTGGGAAG CACATCAGAT
    GACACTGAAG TTAGAGAGGT CAGCTCCCGG CCCAGCACTC CAGGACTCAG TGTGGTGTCA
    GGTGACTTTC CCAAACCTTT CTATTCATTG CCGGGTATCA GTGCTACCTC AGAGGACATC
    CCAAACAAGA TAGAGGACCT ACGCTCAGAG TGCAGCTCTG ACTTTGGAGG AAAGGATTCT
    GTTACAAGTC CTGAGGCAGA AGAGTCTGTT CATGGACCTC ACCATATAAC TGCACCTCCA
    ACCCAGACTG AATCCTTGTT GGCCATGTTT GACCCACTAG CTCCAGCATC CAATGAAGTG
    GTTCGACCTA AAGTACACTA TGCGAGACCC TCACATCCGC CTCCTGACCC GCCTGTACTG
    GAAGGAGCAA GTGGGGGAAA TGAAGCTCGT CTTCCTATGT TCGGTTCTCA TGTATTTGTT
    CCCATGGAGT CTGAAGCTTA CAGGCAGAGA CACTCATGTC CTGAAAGACT GGTGCGCAGT
    CGCAGCTCCG ACATCGCTTC TTCCATACGG AGGCCAATGA GTGACCCTGG CTGGGTGCGG
    CGTGGGGTGA ATGAAGATAG GGAGCTGCCA ACAGGAACTG CAATTAATGC ACTAATTAAT
    CCCCCCCAGG CTTCATCCTC CTCACCAAGC AAGGACTCTT CCAGGGGAGA GCCAGAAGAA
    AAAAAAGACA GCGATGATGA GCGATCAGAT AGGAATAAGC CTTGGTGGAA GAAGCGGTTT
    GTGTCTGCAA TGCCCAAAGC TCCTATCCCA TTTAGGAAGA AGGAAAAACA CGAGAAGGAG
    AGGGAAGACT TGGTACAAGA CAGGTTTTCA GTAGCAGATG ACTCAACATC TCAGCTAGGG
    CCACATTCCC AGGCAGCAGA AGACATCCTG GCTAAGTACA GGAATGCTAT TAAGAGGACA
    ATCCCCAATG AAGGTTCCTC CGTGCCCTAT GAAGGAGCAG ATGCTGTTGG AGATGGAGAA
    AGCCTTCATG ATTCTCCTCG TGATGAAGCT TTGCAGAACA TGGCCTCAGA TGACCTGCTT
    GATTCTGCCA GCCAAGTAGC CCACCCACAG GAGCCTGCAT TCTCCTATAG GGATGCTAAG
    AAAAAATTAC GCCTGGCCCT GTGCTCTGCG GATTCAGTAG CGTTCCCTGT ATTGTCTCAT
    TCGACACGGA ATGGCCTGCC AGATCACACA GACCCAGAAG ACAATGAGAT TGTGTGCTTC
    CTTAAAGTCC AACTTGCAGA GGCCATAAAC CTGCAGGACA AAAACCTGAT GGCTCAGATC
    CAGGAAACTA TGCGATGTGT GAGCCGATTT GACAGCCGCT CCTGCAGCAA AATTTTGGCA
    TCCATTGCAG AGGATTACCG GAAGAGGGCC TCATATATTG CATATCTAAC AAGATGCCGA
    CAGGGACTAC AGACCACCCA GGCTCACCTT GAGCGCCTCC TGCAGAGAGT GCTACGAGAT
    AAAGAGGTCT CCACGCGCTA CTTCACCACT GTCTGTGTGA GGCTTCTACT TGAGAGCAAA
    GAGAAGGAAA TCCATGAATT TATCCAAGAT TTTCAAAAGT TAACTGCAGC TGATGATAAA
    ACAGCACAAG TTGAAGAGTT CTTGCAATCA CTTTACGGGG CTATGGCACA AGATGTGATT
    TGGCAAAATG CCAGTGAGGA GCAGCTGCAA GATGCACAGA TTGCTATTGA GCGAAGCATT
    ATGAACCGCA TCTTTAAGCT GGCCTTCTAT CCCAATCAAG ATGCAGATAT TCTGCGAGAT
    CAGGTCCTGC ATGAACACAT TAAAAGGTTG TCTAAAGTGG TGACTGCCAA TCACAGGGCA
    CTGCAGATAC CTGAGGTGTA TCTGAGGGAG GCTCCTTGGC CATCTGCCCA AACCGAAATC
    CGTACCATCA GCGCATACAA GACACCCAGG GACAAAGTGC AGTGCATATT GCGCATGTGC
    TCAACAATCA TGAACCTTCT AAGCCTGGCC AATGAGTACT CTGTCCCAGG AGCAGATGAT
    TTTGTTCCTG TGCTGGTTTT TGTCCTTATT AAGGCTAATC CCCCCTGCCT ACTATCAACA
    GTGCAATATA TCAGCAACTT CTATTCCAAT CGGCTCACTG GCGAGGAGTC CTACTGGTGG
    ATGCAGTTCA CAGCTGCTGT TGAGTTCATT AAAACCATTG ACGATCGCAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31125
    Clone ID Related Accession (Same CDS sequence) XM_012969067.3 , XM_012969067.2
    Accession Version XM_012969067.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969067.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGAAGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTG ACTTTCCCAA ACCTTTCTAT
    TCATTGCCGG GTATCAGTGC TACCTCAGAG GACATCCCAA ACAAGATAGA GGACCTACGC
    TCAGAGTGCA GCTCTGACTT TGGAGGAAAG GATTCTGTTA CAAGTCCTGA GGCAGAAGAG
    TCTGTTCATG GACCTCACCA TATAACTGCA CCTCCAACCC AGACTGAATC CTTGTTGGCC
    ATGTTTGACC CACTAGCTCC AGCATCCAAT GAAGTGGTTC GACCTAAAGT ACACTATGCG
    AGACCCTCAC ATCCGCCTCC TGACCCGCCT GTACTGGAAG GAGCAAGTGG GGGAAATGAA
    GCTCGTCTTC CTATGTTCGG TTCTCATGTA TTTGTTCCCA TGGAGTCTGA AGCTTACAGG
    CAGAGACACT CATGTCCTGA AAGACTGGTG CGCAGTCGCA GCTCCGACAT CGCTTCTTCC
    ATACGGAGGC CAATGAGTGA CCCTGGCTGG GTGCGGCGTG GGGTGAATGA AGATAGGGAG
    CTGCCAACAG GAACTGCAAT TAATGCACTA ATTAATCCCC CCCAGGCTTC ATCCTCCTCA
    CCAAGCAAGG ACTCTTCCAG GGGAGAGCCA GAAGAAAAAA AAGACAGCGA TGATGAGCGA
    TCAGATAGGA ATAAGCCTTG GTGGAAGAAG CGGTTTGTGT CTGCAATGCC CAAAGCTCCT
    ATCCCATTTA GGAAGAAGGA AAAACACGAG AAGGAGAGGG AAGACTTGGT ACAAGACAGG
    TTTTCAGTAG CAGATGACTC AACATCTCAG CTAGGGCCAC ATTCCCAGGC AGCAGAAGAC
    ATCCTGGCTA AGTACAGGAA TGCTATTAAG AGGACAATCC CCAATGAAGG TTCCTCCGTG
    CCCTATGAAG GAGCAGATGC TGTTGGAGAT GGAGAAAGCC TTCATGATTC TCCTCGTGAT
    GAAGCTTTGC AGAACATGGC CTCAGATGAC CTGCTTGATT CTGCCAGCCA AGTAGCCCAC
    CCACAGGAGC CTGCATTCTC CTATAGGGAT GCTAAGAAAA AATTACGCCT GGCCCTGTGC
    TCTGCGGATT CAGTAGCGTT CCCTGTATTG TCTCATTCGA CACGGAATGG CCTGCCAGAT
    CACACAGACC CAGAAGACAA TGAGATTGTG TGCTTCCTTA AAGTCCAACT TGCAGAGGCC
    ATAAACCTGC AGGACAAAAA CCTGATGGCT CAGATCCAGG AAACTATGCG ATGTGTGAGC
    CGATTTGACA GCCGCTCCTG CAGCAAAATT TTGGCATCCA TTGCAGAGGA TTACCGGAAG
    AGGGCCTCAT ATATTGCATA TCTAACAAGA TGCCGACAGG GACTACAGAC CACCCAGGCT
    CACCTTGAGC GCCTCCTGCA GAGAGTGCTA CGAGATAAAG AGGTCTCCAC GCGCTACTTC
    ACCACTGTCT GTGTGAGGCT TCTACTTGAG AGCAAAGAGA AGGAAATCCA TGAATTTATC
    CAAGATTTTC AAAAGTTAAC TGCAGCTGAT GATAAAACAG CACAAGTTGA AGAGTTCTTG
    CAATCACTTT ACGGGGCTAT GGCACAAGAT GTGATTTGGC AAAATGCCAG TGAGGAGCAG
    CTGCAAGATG CACAGATTGC TATTGAGCGA AGCATTATGA ACCGCATCTT TAAGCTGGCC
    TTCTATCCCA ATCAAGATGC AGATATTCTG CGAGATCAGG TCCTGCATGA ACACATTAAA
    AGGTTGTCTA AAGTGGTGAC TGCCAATCAC AGGGCACTGC AGATACCTGA GGTGTATCTG
    AGGGAGGCTC CTTGGCCATC TGCCCAAACC GAAATCCGTA CCATCAGCGC ATACAAGACA
    CCCAGGGACA AAGTGCAGTG CATATTGCGC ATGTGCTCAA CAATCATGAA CCTTCTAAGC
    CTGGCCAATG AGTACTCTGT CCCAGGAGCA GATGATTTTG TTCCTGTGCT GGTTTTTGTC
    CTTATTAAGG CTAATCCCCC CTGCCTACTA TCAACAGTGC AATATATCAG CAACTTCTAT
    TCCAATCGGC TCACTGGCGA GGAGTCCTAC TGGTGGATGC AGTTCACAGC TGCTGTTGAG
    TTCATTAAAA CCATTGACGA TCGCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824521.1
    CDS170..4639
    Translation

    Target ORF information:

    RefSeq Version XM_012969067.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969067.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGAAGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTG ACTTTCCCAA ACCTTTCTAT
    TCATTGCCGG GTATCAGTGC TACCTCAGAG GACATCCCAA ACAAGATAGA GGACCTACGC
    TCAGAGTGCA GCTCTGACTT TGGAGGAAAG GATTCTGTTA CAAGTCCTGA GGCAGAAGAG
    TCTGTTCATG GACCTCACCA TATAACTGCA CCTCCAACCC AGACTGAATC CTTGTTGGCC
    ATGTTTGACC CACTAGCTCC AGCATCCAAT GAAGTGGTTC GACCTAAAGT ACACTATGCG
    AGACCCTCAC ATCCGCCTCC TGACCCGCCT GTACTGGAAG GAGCAAGTGG GGGAAATGAA
    GCTCGTCTTC CTATGTTCGG TTCTCATGTA TTTGTTCCCA TGGAGTCTGA AGCTTACAGG
    CAGAGACACT CATGTCCTGA AAGACTGGTG CGCAGTCGCA GCTCCGACAT CGCTTCTTCC
    ATACGGAGGC CAATGAGTGA CCCTGGCTGG GTGCGGCGTG GGGTGAATGA AGATAGGGAG
    CTGCCAACAG GAACTGCAAT TAATGCACTA ATTAATCCCC CCCAGGCTTC ATCCTCCTCA
    CCAAGCAAGG ACTCTTCCAG GGGAGAGCCA GAAGAAAAAA AAGACAGCGA TGATGAGCGA
    TCAGATAGGA ATAAGCCTTG GTGGAAGAAG CGGTTTGTGT CTGCAATGCC CAAAGCTCCT
    ATCCCATTTA GGAAGAAGGA AAAACACGAG AAGGAGAGGG AAGACTTGGT ACAAGACAGG
    TTTTCAGTAG CAGATGACTC AACATCTCAG CTAGGGCCAC ATTCCCAGGC AGCAGAAGAC
    ATCCTGGCTA AGTACAGGAA TGCTATTAAG AGGACAATCC CCAATGAAGG TTCCTCCGTG
    CCCTATGAAG GAGCAGATGC TGTTGGAGAT GGAGAAAGCC TTCATGATTC TCCTCGTGAT
    GAAGCTTTGC AGAACATGGC CTCAGATGAC CTGCTTGATT CTGCCAGCCA AGTAGCCCAC
    CCACAGGAGC CTGCATTCTC CTATAGGGAT GCTAAGAAAA AATTACGCCT GGCCCTGTGC
    TCTGCGGATT CAGTAGCGTT CCCTGTATTG TCTCATTCGA CACGGAATGG CCTGCCAGAT
    CACACAGACC CAGAAGACAA TGAGATTGTG TGCTTCCTTA AAGTCCAACT TGCAGAGGCC
    ATAAACCTGC AGGACAAAAA CCTGATGGCT CAGATCCAGG AAACTATGCG ATGTGTGAGC
    CGATTTGACA GCCGCTCCTG CAGCAAAATT TTGGCATCCA TTGCAGAGGA TTACCGGAAG
    AGGGCCTCAT ATATTGCATA TCTAACAAGA TGCCGACAGG GACTACAGAC CACCCAGGCT
    CACCTTGAGC GCCTCCTGCA GAGAGTGCTA CGAGATAAAG AGGTCTCCAC GCGCTACTTC
    ACCACTGTCT GTGTGAGGCT TCTACTTGAG AGCAAAGAGA AGGAAATCCA TGAATTTATC
    CAAGATTTTC AAAAGTTAAC TGCAGCTGAT GATAAAACAG CACAAGTTGA AGAGTTCTTG
    CAATCACTTT ACGGGGCTAT GGCACAAGAT GTGATTTGGC AAAATGCCAG TGAGGAGCAG
    CTGCAAGATG CACAGATTGC TATTGAGCGA AGCATTATGA ACCGCATCTT TAAGCTGGCC
    TTCTATCCCA ATCAAGATGC AGATATTCTG CGAGATCAGG TCCTGCATGA ACACATTAAA
    AGGTTGTCTA AAGTGGTGAC TGCCAATCAC AGGGCACTGC AGATACCTGA GGTGTATCTG
    AGGGAGGCTC CTTGGCCATC TGCCCAAACC GAAATCCGTA CCATCAGCGC ATACAAGACA
    CCCAGGGACA AAGTGCAGTG CATATTGCGC ATGTGCTCAA CAATCATGAA CCTTCTAAGC
    CTGGCCAATG AGTACTCTGT CCCAGGAGCA GATGATTTTG TTCCTGTGCT GGTTTTTGTC
    CTTATTAAGG CTAATCCCCC CTGCCTACTA TCAACAGTGC AATATATCAG CAACTTCTAT
    TCCAATCGGC TCACTGGCGA GGAGTCCTAC TGGTGGATGC AGTTCACAGC TGCTGTTGAG
    TTCATTAAAA CCATTGACGA TCGCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31127
    Clone ID Related Accession (Same CDS sequence) XM_012969069.2 , XM_012969069.3
    Accession Version XM_012969069.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969069.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGAAGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTA TCAGTGCTAC CTCAGAGGAC
    ATCCCAAACA AGATAGAGGA CCTACGCTCA GAGTGCAGCT CTGACTTTGG AGGAAAGGAT
    TCTGTTACAA GTCCTGAGGC AGAAGAGTCT GTTCATGGAC CTCACCATAT AACTGCACCT
    CCAACCCAGA CTGAATCCTT GTTGGCCATG TTTGACCCAC TAGCTCCAGC ATCCAATGAA
    GTGGTTCGAC CTAAAGTACA CTATGCGAGA CCCTCACATC CGCCTCCTGA CCCGCCTGTA
    CTGGAAGGAG CAAGTGGGGG AAATGAAGCT CGTCTTCCTA TGTTCGGTTC TCATGTATTT
    GTTCCCATGG AGTCTGAAGC TTACAGGCAG AGACACTCAT GTCCTGAAAG ACTGGTGCGC
    AGTCGCAGCT CCGACATCGC TTCTTCCATA CGGAGGCCAA TGAGTGACCC TGGCTGGGTG
    CGGCGTGGGG TGAATGAAGA TAGGGAGCTG CCAACAGGAA CTGCAATTAA TGCACTAATT
    AATCCCCCCC AGGCTTCATC CTCCTCACCA AGCAAGGACT CTTCCAGGGG AGAGCCAGAA
    GAAAAAAAAG ACAGCGATGA TGAGCGATCA GATAGGAATA AGCCTTGGTG GAAGAAGCGG
    TTTGTGTCTG CAATGCCCAA AGCTCCTATC CCATTTAGGA AGAAGGAAAA ACACGAGAAG
    GAGAGGGAAG ACTTGGTACA AGACAGGTTT TCAGTAGCAG ATGACTCAAC ATCTCAGCTA
    GGGCCACATT CCCAGGCAGC AGAAGACATC CTGGCTAAGT ACAGGAATGC TATTAAGAGG
    ACAATCCCCA ATGAAGGTTC CTCCGTGCCC TATGAAGGAG CAGATGCTGT TGGAGATGGA
    GAAAGCCTTC ATGATTCTCC TCGTGATGAA GCTTTGCAGA ACATGGCCTC AGATGACCTG
    CTTGATTCTG CCAGCCAAGT AGCCCACCCA CAGGAGCCTG CATTCTCCTA TAGGGATGCT
    AAGAAAAAAT TACGCCTGGC CCTGTGCTCT GCGGATTCAG TAGCGTTCCC TGTATTGTCT
    CATTCGACAC GGAATGGCCT GCCAGATCAC ACAGACCCAG AAGACAATGA GATTGTGTGC
    TTCCTTAAAG TCCAACTTGC AGAGGCCATA AACCTGCAGG ACAAAAACCT GATGGCTCAG
    ATCCAGGAAA CTATGCGATG TGTGAGCCGA TTTGACAGCC GCTCCTGCAG CAAAATTTTG
    GCATCCATTG CAGAGGATTA CCGGAAGAGG GCCTCATATA TTGCATATCT AACAAGATGC
    CGACAGGGAC TACAGACCAC CCAGGCTCAC CTTGAGCGCC TCCTGCAGAG AGTGCTACGA
    GATAAAGAGG TCTCCACGCG CTACTTCACC ACTGTCTGTG TGAGGCTTCT ACTTGAGAGC
    AAAGAGAAGG AAATCCATGA ATTTATCCAA GATTTTCAAA AGTTAACTGC AGCTGATGAT
    AAAACAGCAC AAGTTGAAGA GTTCTTGCAA TCACTTTACG GGGCTATGGC ACAAGATGTG
    ATTTGGCAAA ATGCCAGTGA GGAGCAGCTG CAAGATGCAC AGATTGCTAT TGAGCGAAGC
    ATTATGAACC GCATCTTTAA GCTGGCCTTC TATCCCAATC AAGATGCAGA TATTCTGCGA
    GATCAGGTCC TGCATGAACA CATTAAAAGG TTGTCTAAAG TGGTGACTGC CAATCACAGG
    GCACTGCAGA TACCTGAGGT GTATCTGAGG GAGGCTCCTT GGCCATCTGC CCAAACCGAA
    ATCCGTACCA TCAGCGCATA CAAGACACCC AGGGACAAAG TGCAGTGCAT ATTGCGCATG
    TGCTCAACAA TCATGAACCT TCTAAGCCTG GCCAATGAGT ACTCTGTCCC AGGAGCAGAT
    GATTTTGTTC CTGTGCTGGT TTTTGTCCTT ATTAAGGCTA ATCCCCCCTG CCTACTATCA
    ACAGTGCAAT ATATCAGCAA CTTCTATTCC AATCGGCTCA CTGGCGAGGA GTCCTACTGG
    TGGATGCAGT TCACAGCTGC TGTTGAGTTC ATTAAAACCA TTGACGATCG CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824523.1
    CDS168..4604
    Translation

    Target ORF information:

    RefSeq Version XM_012969069.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969069.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GGTCAGCAGC TGGCAGCCAT GACAGTATGG GACACATCTG CCTCAAATCT CACCGCTCAC
    ATTAACCTGG TGACCCCGTT TGTTACTCGT AGTAGAAGCA AAACAAGCCT GATGGAAGCA
    GAGAATGAGG GATCTCCGCA GGAAGTCACT CCTGGTCAGC CTGAAGAAGT CCTGGTCATC
    TCACTAGGGA CTGGTCCCCA GCTCACACCA GGAATGATGT CTGAAAATGA GGTTTTGAAC
    ATGCAGCTGG TAGATGGGGG TCAGGTGGAC GTGCCTGTGG ATGAATCTAA ACTTCATGGA
    AAGCCAGACA AAACCCTGCG TTTCTCACTT TGCAGTGACA ACCTAGAAGG CATTTCAGAA
    GGTCCTTCAA ATCGTTCCAA CTCAGTTTCC TCTTTGGATC TGGAAGGGGA GTCTGTATCT
    GAGCTAGGAG GAGGACCATC CGGAAGCAAT GGGGTGGAGG CTTTGCAGTT ATTGGAACAT
    GAACAAGCTA CCACACAGGA TAATCTAGAT GACAAACTGC GCAAATTTGA AATTCGAGAC
    ATGATGGGAT TGACGGATGA TAGAGATATA TCAGAGACAG TCAGTGAGAC ATGGAGCACG
    GATGTGCTTG GAAGTGATTT TGACCCCAAC ATTGATGAAG ATCGACTACA AGAAATTGCA
    GGTGCAGCAG CTGAGAACAT GCTGGGAAGC CTGCTGTGCC TTCCCGGCTC TGGCTCAATA
    CTGCTTGATC CTTGTACTGG ATCCACTATA TCTGAGACTA CCAGTGAAGC ATGGAGTGTT
    GAGGTGCTCC CTAGTGATTC TGAAGCACCA GATCTAAAGC AAGAGGAGCG GCTGCAGGAA
    CTGGAGAGCT GTTCAGGTTT GGGAAGCACA TCAGATGACA CTGAAGTTAG AGAGGTCAGC
    TCCCGGCCCA GCACTCCAGG ACTCAGTGTG GTGTCAGGTA TCAGTGCTAC CTCAGAGGAC
    ATCCCAAACA AGATAGAGGA CCTACGCTCA GAGTGCAGCT CTGACTTTGG AGGAAAGGAT
    TCTGTTACAA GTCCTGAGGC AGAAGAGTCT GTTCATGGAC CTCACCATAT AACTGCACCT
    CCAACCCAGA CTGAATCCTT GTTGGCCATG TTTGACCCAC TAGCTCCAGC ATCCAATGAA
    GTGGTTCGAC CTAAAGTACA CTATGCGAGA CCCTCACATC CGCCTCCTGA CCCGCCTGTA
    CTGGAAGGAG CAAGTGGGGG AAATGAAGCT CGTCTTCCTA TGTTCGGTTC TCATGTATTT
    GTTCCCATGG AGTCTGAAGC TTACAGGCAG AGACACTCAT GTCCTGAAAG ACTGGTGCGC
    AGTCGCAGCT CCGACATCGC TTCTTCCATA CGGAGGCCAA TGAGTGACCC TGGCTGGGTG
    CGGCGTGGGG TGAATGAAGA TAGGGAGCTG CCAACAGGAA CTGCAATTAA TGCACTAATT
    AATCCCCCCC AGGCTTCATC CTCCTCACCA AGCAAGGACT CTTCCAGGGG AGAGCCAGAA
    GAAAAAAAAG ACAGCGATGA TGAGCGATCA GATAGGAATA AGCCTTGGTG GAAGAAGCGG
    TTTGTGTCTG CAATGCCCAA AGCTCCTATC CCATTTAGGA AGAAGGAAAA ACACGAGAAG
    GAGAGGGAAG ACTTGGTACA AGACAGGTTT TCAGTAGCAG ATGACTCAAC ATCTCAGCTA
    GGGCCACATT CCCAGGCAGC AGAAGACATC CTGGCTAAGT ACAGGAATGC TATTAAGAGG
    ACAATCCCCA ATGAAGGTTC CTCCGTGCCC TATGAAGGAG CAGATGCTGT TGGAGATGGA
    GAAAGCCTTC ATGATTCTCC TCGTGATGAA GCTTTGCAGA ACATGGCCTC AGATGACCTG
    CTTGATTCTG CCAGCCAAGT AGCCCACCCA CAGGAGCCTG CATTCTCCTA TAGGGATGCT
    AAGAAAAAAT TACGCCTGGC CCTGTGCTCT GCGGATTCAG TAGCGTTCCC TGTATTGTCT
    CATTCGACAC GGAATGGCCT GCCAGATCAC ACAGACCCAG AAGACAATGA GATTGTGTGC
    TTCCTTAAAG TCCAACTTGC AGAGGCCATA AACCTGCAGG ACAAAAACCT GATGGCTCAG
    ATCCAGGAAA CTATGCGATG TGTGAGCCGA TTTGACAGCC GCTCCTGCAG CAAAATTTTG
    GCATCCATTG CAGAGGATTA CCGGAAGAGG GCCTCATATA TTGCATATCT AACAAGATGC
    CGACAGGGAC TACAGACCAC CCAGGCTCAC CTTGAGCGCC TCCTGCAGAG AGTGCTACGA
    GATAAAGAGG TCTCCACGCG CTACTTCACC ACTGTCTGTG TGAGGCTTCT ACTTGAGAGC
    AAAGAGAAGG AAATCCATGA ATTTATCCAA GATTTTCAAA AGTTAACTGC AGCTGATGAT
    AAAACAGCAC AAGTTGAAGA GTTCTTGCAA TCACTTTACG GGGCTATGGC ACAAGATGTG
    ATTTGGCAAA ATGCCAGTGA GGAGCAGCTG CAAGATGCAC AGATTGCTAT TGAGCGAAGC
    ATTATGAACC GCATCTTTAA GCTGGCCTTC TATCCCAATC AAGATGCAGA TATTCTGCGA
    GATCAGGTCC TGCATGAACA CATTAAAAGG TTGTCTAAAG TGGTGACTGC CAATCACAGG
    GCACTGCAGA TACCTGAGGT GTATCTGAGG GAGGCTCCTT GGCCATCTGC CCAAACCGAA
    ATCCGTACCA TCAGCGCATA CAAGACACCC AGGGACAAAG TGCAGTGCAT ATTGCGCATG
    TGCTCAACAA TCATGAACCT TCTAAGCCTG GCCAATGAGT ACTCTGTCCC AGGAGCAGAT
    GATTTTGTTC CTGTGCTGGT TTTTGTCCTT ATTAAGGCTA ATCCCCCCTG CCTACTATCA
    ACAGTGCAAT ATATCAGCAA CTTCTATTCC AATCGGCTCA CTGGCGAGGA GTCCTACTGG
    TGGATGCAGT TCACAGCTGC TGTTGAGTTC ATTAAAACCA TTGACGATCG CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31129
    Clone ID Related Accession (Same CDS sequence) XM_012969071.2
    Accession Version XM_012969071.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4413bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969071.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG AAGAGGAAAA TCCGTGTTCA
    ACAGGTCCTT CAAATCGTTC CAACTCAGTT TCCTCTTTGG ATCTGGAAGG GGAGTCTGTA
    TCTGAGCTAG GAGGAGGACC ATCCGGAAGC AATGGGGTGG AGGCTTTGCA GTTATTGGAA
    CATGAACAAG CTACCACACA GGATAATCTA GATGACAAAC TGCGCAAATT TGAAATTCGA
    GACATGATGG GATTGACGGA TGATAGAGAT ATATCAGAGA CAGTCAGTGA GACATGGAGC
    ACGGATGTGC TTGGAAGTGA TTTTGACCCC AACATTGATG AAGATCGACT ACAAGAAATT
    GCAGGTGCAG CAGCTGAGAA CATGCTGGGA AGCCTGCTGT GCCTTCCCGG CTCTGGCTCA
    ATACTGCTTG ATCCTTGTAC TGGATCCACT ATATCTGAGA CTACCAGTGA AGCATGGAGT
    GTTGAGGTGC TCCCTAGTGA TTCTGAAGCA CCAGATCTAA AGCAAGAGGA GCGGCTGCAG
    GAACTGGAGA GCTGTTCAGG TTTGGGAAGC ACATCAGATG ACACTGAAGT TAGAGAGGTC
    AGCTCCCGGC CCAGCACTCC AGGACTCAGT GTGGTGTCAG GTGACTTTCC CAAACCTTTC
    TATTCATTGC CGGGTATCAG TGCTACCTCA GAGGACATCC CAAACAAGAT AGAGGACCTA
    CGCTCAGAGT GCAGCTCTGA CTTTGGAGGA AAGGATTCTG TTACAAGTCC TGAGGCAGAA
    GAGTCTGTTC ATGGACCTCA CCATATAACT GCACCTCCAA CCCAGACTGA ATCCTTGTTG
    GCCATGTTTG ACCCACTAGC TCCAGCATCC AATGAAGTGG TTCGACCTAA AGTACACTAT
    GCGAGACCCT CACATCCGCC TCCTGACCCG CCTGTACTGG AAGGAGCAAG TGGGGGAAAT
    GAAGCTCGTC TTCCTATGTT CGGTTCTCAT GTATTTGTTC CCATGGAGTC TGAAGCTTAC
    AGGCAGAGAC ACTCATGTCC TGAAAGACTG GTGCGCAGTC GCAGCTCCGA CATCGCTTCT
    TCCATACGGA GGCCAATGAG TGACCCTGGC TGGGTGCGGC GTGGGGTGAA TGAAGATAGG
    GAGCTGCCAA CAGGAACTGC AATTAATGCA CTAATTAATC CCCCCCAGGC TTCATCCTCC
    TCACCAAGCA AGGACTCTTC CAGGGGAGAG CCAGAAGAAA AAAAAGACAG CGATGATGAG
    CGATCAGATA GGAATAAGCC TTGGTGGAAG AAGCGGTTTG TGTCTGCAAT GCCCAAAGCT
    CCTATCCCAT TTAGGAAGAA GGAAAAACAC GAGAAGGAGA GGGAAGACTT GGTACAAGAC
    AGGTTTTCAG TAGCAGATGA CTCAACATCT CAGCTAGGGC CACATTCCCA GGCAGCAGAA
    GACATCCTGG CTAAGTACAG GAATGCTATT AAGAGGACAA TCCCCAATGA AGGTTCCTCC
    GTGCCCTATG AAGGAGCAGA TGCTGTTGGA GATGGAGAAA GCCTTCATGA TTCTCCTCGT
    GATGAAGCTT TGCAGAACAT GGCCTCAGAT GACCTGCTTG ATTCTGCCAG CCAAGTAGCC
    CACCCACAGG AGCCTGCATT CTCCTATAGG GATGCTAAGA AAAAATTACG CCTGGCCCTG
    TGCTCTGCGG ATTCAGTAGC GTTCCCTGTA TTGTCTCATT CGACACGGAA TGGCCTGCCA
    GATCACACAG ACCCAGAAGA CAATGAGATT GTGTGCTTCC TTAAAGTCCA ACTTGCAGAG
    GCCATAAACC TGCAGGACAA AAACCTGATG GCTCAGATCC AGGAAACTAT GCGATGTGTG
    AGCCGATTTG ACAGCCGCTC CTGCAGCAAA ATTTTGGCAT CCATTGCAGA GGATTACCGG
    AAGAGGGCCT CATATATTGC ATATCTAACA AGATGCCGAC AGGGACTACA GACCACCCAG
    GCTCACCTTG AGCGCCTCCT GCAGAGAGTG CTACGAGATA AAGAGGTCTC CACGCGCTAC
    TTCACCACTG TCTGTGTGAG GCTTCTACTT GAGAGCAAAG AGAAGGAAAT CCATGAATTT
    ATCCAAGATT TTCAAAAGTT AACTGCAGCT GATGATAAAA CAGCACAAGT TGAAGAGTTC
    TTGCAATCAC TTTACGGGGC TATGGCACAA GATGTGATTT GGCAAAATGC CAGTGAGGAG
    CAGCTGCAAG ATGCACAGAT TGCTATTGAG CGAAGCATTA TGAACCGCAT CTTTAAGCTG
    GCCTTCTATC CCAATCAAGA TGCAGATATT CTGCGAGATC AGGTCCTGCA TGAACACATT
    AAAAGGTTGT CTAAAGTGGT GACTGCCAAT CACAGGGCAC TGCAGATACC TGAGGTGTAT
    CTGAGGGAGG CTCCTTGGCC ATCTGCCCAA ACCGAAATCC GTACCATCAG CGCATACAAG
    ACACCCAGGG ACAAAGTGCA GTGCATATTG CGCATGTGCT CAACAATCAT GAACCTTCTA
    AGCCTGGCCA ATGAGTACTC TGTCCCAGGA GCAGATGATT TTGTTCCTGT GCTGGTTTTT
    GTCCTTATTA AGGCTAATCC CCCCTGCCTA CTATCAACAG TGCAATATAT CAGCAACTTC
    TATTCCAATC GGCTCACTGG CGAGGAGTCC TACTGGTGGA TGCAGTTCAC AGCTGCTGTT
    GAGTTCATTA AAACCATTGA CGATCGCAAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824525.1
    CDS169..4581
    Translation

    Target ORF information:

    RefSeq Version XM_012969071.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GTPase activating protein and VPS9 domains 1 (gapvd1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969071.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTAAAGC CGGATATCCA CACTCTTGCT CGCCATCTGA AGCAAGAGCG GCTCTATGTG 
    AACTCAGAGA AGCAGCTCAT CCAGAGGCTG AATGCTGATG TGCTGAAGAC AGCAGAAAGG
    CTCTACCGAA CATCATGGAT AGCCAAGCAA CAAAGAATTA ACCTGGACAG ACTCATCCTC
    ACAAGCGCTG AAGCATCTCC AGCTGAGTGT TGCCAACATG CAAAGGTCCT GGAAGATACT
    CAGTTTGTGG ATGGTTACAA GCAGCTGGGC TTCCAAGAGA CTGCATATGG AGAGTTTCTT
    AATCGCCTGC GTGAGAACCC ACGCCTCATT GCATCTTGCC TCGTGGCTGG AGAAAAACTC
    AACCATGAGA ATGCCCAGAG TGTCATACAC ACTGTCTTTA CCTCTCTGTA TGGCAACTGT
    ATAATGCAGG AGGATGAGAG CTACTTGTTA CAGGTCCTGC GTTACTTAAT TGAGTTTGAA
    CTGAAGGAGA GTGATAACCC CCAAAGGCTG CTCAGGAGAG GCACATGTGC TTTTAGCATT
    ATCTTCAAAC TTTTCTCTGA AGGCCTTTTC TCCGCCAAGC TCTTCCTAAC AGCAACCCTA
    CATGAGCCGA TCATGCAGTT GCTGGTGGAA GATGAGGACC ACCTAGAAAC TGACCCTTCA
    AAATTGATTG AGCGGTTTTC TCCAGCCCAG CAGGAAAAGT TGTTTGGGGA GAAGGGCAGT
    GAGGCTTTTC GTCAAAGGGT TCAGGAAATG GTGGAGTCAA ATGAAGCTAA GCTTGTGACA
    CTTGTCAATA AATTCATAGG ATACTTGAAG CAGAACACAT ACTGCTTCCC ACACAGTCTA
    AGGTGGATCG TCTCACAGAT GTTTAAAACT TTGTCCTGTG TGGAGGGGCT GGAGGTTAGT
    GAGGTGAGAT CTATGTGCAC CGACCTGCTA CTGACTTGTT TCATCTGCCC AGCAATTGTG
    AACCCAGAGC AGTATGGGAT AATTTCAGAT GCTCCAATCA ATGAAGTGGC AAGGTTCAAC
    TTAATGCAGG TGGGGCGTCT CTTACAGCAG CTGGCACTAA CTGGATTTGA GGAGGGAGAT
    TCACGGAACA AGAATAACCT CAGTAAATTT GACAAAAGTT GTGTGGCTGC TTTCCTGGAT
    GTGGTTATTG GTGGTCGAGC AGTTGAAACA CCTCCCCTTT CATCTGTGAA TCTCTTGGAA
    GGATTAAGTC GAACAGTTGT ATACATGACA TACAGCCAAC TTATGAGTCT ATTGGGGTTC
    TTGCGTACTG TCATCTCCAG TGACCAACTG CGGGAGGAGG ACAGGATGGC ACTGGAAAAC
    CTCCTGGCTA CAGTGCCCCA GACCAAGCCA GGGAAGAGTA ACAATGATAC TACACCTTAC
    AGCACCCCTC AGCTCTCACC AGCCACAACA CCAGCCTGTA AGAAGAACAG GCTACCTATA
    GTTACTCGTA GTAGAAGCAA AACAAGCCTG ATGGAAGCAG AGAATGAGGG ATCTCCGCAG
    GAAGTCACTC CTGGTCAGCC TGAAGAAGTC CTGGTCATCT CACTAGGGAC TGGTCCCCAG
    CTCACACCAG GAATGATGTC TGAAAATGAG GTTTTGAACA TGCAGCTGGT AGATGGGGGT
    CAGGTGGACG TGCCTGTGGA TGAATCTAAA CTTCATGGAA AGCCAGACAA AACCCTGCGT
    TTCTCACTTT GCAGTGACAA CCTAGAAGGC ATTTCAGAAG AAGAGGAAAA TCCGTGTTCA
    ACAGGTCCTT CAAATCGTTC CAACTCAGTT TCCTCTTTGG ATCTGGAAGG GGAGTCTGTA
    TCTGAGCTAG GAGGAGGACC ATCCGGAAGC AATGGGGTGG AGGCTTTGCA GTTATTGGAA
    CATGAACAAG CTACCACACA GGATAATCTA GATGACAAAC TGCGCAAATT TGAAATTCGA
    GACATGATGG GATTGACGGA TGATAGAGAT ATATCAGAGA CAGTCAGTGA GACATGGAGC
    ACGGATGTGC TTGGAAGTGA TTTTGACCCC AACATTGATG AAGATCGACT ACAAGAAATT
    GCAGGTGCAG CAGCTGAGAA CATGCTGGGA AGCCTGCTGT GCCTTCCCGG CTCTGGCTCA
    ATACTGCTTG ATCCTTGTAC TGGATCCACT ATATCTGAGA CTACCAGTGA AGCATGGAGT
    GTTGAGGTGC TCCCTAGTGA TTCTGAAGCA CCAGATCTAA AGCAAGAGGA GCGGCTGCAG
    GAACTGGAGA GCTGTTCAGG TTTGGGAAGC ACATCAGATG ACACTGAAGT TAGAGAGGTC
    AGCTCCCGGC CCAGCACTCC AGGACTCAGT GTGGTGTCAG GTGACTTTCC CAAACCTTTC
    TATTCATTGC CGGGTATCAG TGCTACCTCA GAGGACATCC CAAACAAGAT AGAGGACCTA
    CGCTCAGAGT GCAGCTCTGA CTTTGGAGGA AAGGATTCTG TTACAAGTCC TGAGGCAGAA
    GAGTCTGTTC ATGGACCTCA CCATATAACT GCACCTCCAA CCCAGACTGA ATCCTTGTTG
    GCCATGTTTG ACCCACTAGC TCCAGCATCC AATGAAGTGG TTCGACCTAA AGTACACTAT
    GCGAGACCCT CACATCCGCC TCCTGACCCG CCTGTACTGG AAGGAGCAAG TGGGGGAAAT
    GAAGCTCGTC TTCCTATGTT CGGTTCTCAT GTATTTGTTC CCATGGAGTC TGAAGCTTAC
    AGGCAGAGAC ACTCATGTCC TGAAAGACTG GTGCGCAGTC GCAGCTCCGA CATCGCTTCT
    TCCATACGGA GGCCAATGAG TGACCCTGGC TGGGTGCGGC GTGGGGTGAA TGAAGATAGG
    GAGCTGCCAA CAGGAACTGC AATTAATGCA CTAATTAATC CCCCCCAGGC TTCATCCTCC
    TCACCAAGCA AGGACTCTTC CAGGGGAGAG CCAGAAGAAA AAAAAGACAG CGATGATGAG
    CGATCAGATA GGAATAAGCC TTGGTGGAAG AAGCGGTTTG TGTCTGCAAT GCCCAAAGCT
    CCTATCCCAT TTAGGAAGAA GGAAAAACAC GAGAAGGAGA GGGAAGACTT GGTACAAGAC
    AGGTTTTCAG TAGCAGATGA CTCAACATCT CAGCTAGGGC CACATTCCCA GGCAGCAGAA
    GACATCCTGG CTAAGTACAG GAATGCTATT AAGAGGACAA TCCCCAATGA AGGTTCCTCC
    GTGCCCTATG AAGGAGCAGA TGCTGTTGGA GATGGAGAAA GCCTTCATGA TTCTCCTCGT
    GATGAAGCTT TGCAGAACAT GGCCTCAGAT GACCTGCTTG ATTCTGCCAG CCAAGTAGCC
    CACCCACAGG AGCCTGCATT CTCCTATAGG GATGCTAAGA AAAAATTACG CCTGGCCCTG
    TGCTCTGCGG ATTCAGTAGC GTTCCCTGTA TTGTCTCATT CGACACGGAA TGGCCTGCCA
    GATCACACAG ACCCAGAAGA CAATGAGATT GTGTGCTTCC TTAAAGTCCA ACTTGCAGAG
    GCCATAAACC TGCAGGACAA AAACCTGATG GCTCAGATCC AGGAAACTAT GCGATGTGTG
    AGCCGATTTG ACAGCCGCTC CTGCAGCAAA ATTTTGGCAT CCATTGCAGA GGATTACCGG
    AAGAGGGCCT CATATATTGC ATATCTAACA AGATGCCGAC AGGGACTACA GACCACCCAG
    GCTCACCTTG AGCGCCTCCT GCAGAGAGTG CTACGAGATA AAGAGGTCTC CACGCGCTAC
    TTCACCACTG TCTGTGTGAG GCTTCTACTT GAGAGCAAAG AGAAGGAAAT CCATGAATTT
    ATCCAAGATT TTCAAAAGTT AACTGCAGCT GATGATAAAA CAGCACAAGT TGAAGAGTTC
    TTGCAATCAC TTTACGGGGC TATGGCACAA GATGTGATTT GGCAAAATGC CAGTGAGGAG
    CAGCTGCAAG ATGCACAGAT TGCTATTGAG CGAAGCATTA TGAACCGCAT CTTTAAGCTG
    GCCTTCTATC CCAATCAAGA TGCAGATATT CTGCGAGATC AGGTCCTGCA TGAACACATT
    AAAAGGTTGT CTAAAGTGGT GACTGCCAAT CACAGGGCAC TGCAGATACC TGAGGTGTAT
    CTGAGGGAGG CTCCTTGGCC ATCTGCCCAA ACCGAAATCC GTACCATCAG CGCATACAAG
    ACACCCAGGG ACAAAGTGCA GTGCATATTG CGCATGTGCT CAACAATCAT GAACCTTCTA
    AGCCTGGCCA ATGAGTACTC TGTCCCAGGA GCAGATGATT TTGTTCCTGT GCTGGTTTTT
    GTCCTTATTA AGGCTAATCC CCCCTGCCTA CTATCAACAG TGCAATATAT CAGCAACTTC
    TATTCCAATC GGCTCACTGG CGAGGAGTCC TACTGGTGGA TGCAGTTCAC AGCTGCTGTT
    GAGTTCATTA AAACCATTGA CGATCGCAAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.