GAPVD1 cDNA ORF clone, Bos taurus(cattle)

The following GAPVD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAPVD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa220565 XM_024999021.1
Latest version!
Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa220566 XM_024999022.1
Latest version!
Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa44066 NM_001102520.1
Latest version!
Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa54402 XM_010810351.3
Latest version!
Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa54403 XM_010810352.3
Latest version!
Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa54404 XM_010810353.3
Latest version!
Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa54405 XM_015473586.1
Latest version!
Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa54405 XM_024999023.1
Latest version!
Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa54406 XM_010810355.3
Latest version!
Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa54408 XM_015473587.1
Latest version!
Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa54408 XM_024999024.1
Latest version!
Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa54403 XM_010810352.2 Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa54402 XM_010810351.2 Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa54404 XM_010810353.2 Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa54406 XM_010810355.2 Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa220565
    Clone ID Related Accession (Same CDS sequence) XM_024999021.1
    Accession Version XM_024999021.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGCT CCTATACCAT TTAGAAAGAA AGAAAAACAA
    GAAAAAGACA AAGATGATCT GGGGCCTGAC AGATTCTCAA CACTCACAGA TGATCCCAGC
    CCGAGACTCA GTGCACAGGC TCAGGTCGCT GAGGATATTC TGGACAAATA CAGGAATGCC
    ATCAAACGCA CCAGCCCCAG TGAGGGAGCT CTGGCAAACT ACGAAAGTGC AGTCATGGGT
    GACGGTGAAA GCGCACACGA CTCTCCTCGT GACGAAACCC TGCAGAACAT CTCCGCTGAC
    GATCTCCCAG ATTCTGCGAG CCAAGCGGCT CACCCTCAGG ATTCAGCCTT CTCTTACAGA
    GATGCAAAAA AGAAACTGAG GCTCGCTCTT TGCTCTGCGG ATTCTGTGGC TTTCCCAGTG
    CTAACCCACT CAACAAGAAA CGGTTTACCA GACCATACAG ACCCAGAAGA CAATGAAATT
    GTCTGCTTCT TAAAAGTGCA AATAGCTGAA GCAATTAACT TACAAGATAA GAACTTAATG
    GCTCAGCTTC AGGAAACAAT GCGTTGTGTG TGCCGCTTTG ATAACCGGAC CTGTAGGAAG
    CTGCTGGCGT CCATCGCCGA GGACTACAGC GTCTTTCCTT TCAGGAAGCG GGCCCCCTAC
    ATTGCTTACC TCACTCGCTG TCGACAAGGA CTGCAGACCA CGCAGGCTCA CCTGGAAAGG
    CTACTGCAGA GGGTTCTGCG GGACAAAGAG GTGGCCAATC GATACTTCAC CACCGTCTGT
    GTGAGGCTGC TGCTTGAGAG CAAAGAGAAG AAGATCAGGG AATTCATTCA AGACTTTCAG
    AAACTCACAG CCGCTGATGA TAAAACCGCC CAAGTGGAAG ATTTTCTGCA GTTCCTGTAC
    GGCGCGATGG CCCAGGACGT CATATGGCAG AACGCGAGTG AGGAACAGCT TCAGGACGCT
    CAGCTGGCCA TTGAGAGAAG TGTGATGAAC CGGATCTTCA AACTTGCCTT CTACCCAAAT
    CAGGACGGGG ACATACTCCG TGACCAGGTT CTTCATGAAC ATATTCAGAG ATTGTCTAAA
    GTAGTGACTG CAAATCACAG AGCTCTGCAG ATACCAGAGG TTTATCTTAA GGAGGCACCG
    TGGCCCTCGG CACAGTCAGA AATCAGGACA ATAAGTGCTT ACAAGACGCC CCGGGACAAA
    GTGCAGTGCA TCCTGAGAAT GTGCTCCACG ATCATGAACC TCCTGAGCCT GGCCAACGAG
    GACTCCGTCC CAGGGGCAGA TGACTTCGTC CCTGTGCTGG TGTTTGTGTT GATCAAGGCA
    AATCCACCCT GTTTGCTGTC CACTGTTCAG TACATCAGTA GCTTTTATGC CAGCTGTCTG
    TCTGGAGAGG AGTCCTATTG GTGGATGCAG TTCACGGCGG CCGTGGAATT CATTAAGACT
    ATCGATGACC GAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854789.1
    CDS184..4581
    Translation

    Target ORF information:

    RefSeq Version XM_024999021.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999021.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGCT CCTATACCAT TTAGAAAGAA AGAAAAACAA
    GAAAAAGACA AAGATGATCT GGGGCCTGAC AGATTCTCAA CACTCACAGA TGATCCCAGC
    CCGAGACTCA GTGCACAGGC TCAGGTCGCT GAGGATATTC TGGACAAATA CAGGAATGCC
    ATCAAACGCA CCAGCCCCAG TGAGGGAGCT CTGGCAAACT ACGAAAGTGC AGTCATGGGT
    GACGGTGAAA GCGCACACGA CTCTCCTCGT GACGAAACCC TGCAGAACAT CTCCGCTGAC
    GATCTCCCAG ATTCTGCGAG CCAAGCGGCT CACCCTCAGG ATTCAGCCTT CTCTTACAGA
    GATGCAAAAA AGAAACTGAG GCTCGCTCTT TGCTCTGCGG ATTCTGTGGC TTTCCCAGTG
    CTAACCCACT CAACAAGAAA CGGTTTACCA GACCATACAG ACCCAGAAGA CAATGAAATT
    GTCTGCTTCT TAAAAGTGCA AATAGCTGAA GCAATTAACT TACAAGATAA GAACTTAATG
    GCTCAGCTTC AGGAAACAAT GCGTTGTGTG TGCCGCTTTG ATAACCGGAC CTGTAGGAAG
    CTGCTGGCGT CCATCGCCGA GGACTACAGC GTCTTTCCTT TCAGGAAGCG GGCCCCCTAC
    ATTGCTTACC TCACTCGCTG TCGACAAGGA CTGCAGACCA CGCAGGCTCA CCTGGAAAGG
    CTACTGCAGA GGGTTCTGCG GGACAAAGAG GTGGCCAATC GATACTTCAC CACCGTCTGT
    GTGAGGCTGC TGCTTGAGAG CAAAGAGAAG AAGATCAGGG AATTCATTCA AGACTTTCAG
    AAACTCACAG CCGCTGATGA TAAAACCGCC CAAGTGGAAG ATTTTCTGCA GTTCCTGTAC
    GGCGCGATGG CCCAGGACGT CATATGGCAG AACGCGAGTG AGGAACAGCT TCAGGACGCT
    CAGCTGGCCA TTGAGAGAAG TGTGATGAAC CGGATCTTCA AACTTGCCTT CTACCCAAAT
    CAGGACGGGG ACATACTCCG TGACCAGGTT CTTCATGAAC ATATTCAGAG ATTGTCTAAA
    GTAGTGACTG CAAATCACAG AGCTCTGCAG ATACCAGAGG TTTATCTTAA GGAGGCACCG
    TGGCCCTCGG CACAGTCAGA AATCAGGACA ATAAGTGCTT ACAAGACGCC CCGGGACAAA
    GTGCAGTGCA TCCTGAGAAT GTGCTCCACG ATCATGAACC TCCTGAGCCT GGCCAACGAG
    GACTCCGTCC CAGGGGCAGA TGACTTCGTC CCTGTGCTGG TGTTTGTGTT GATCAAGGCA
    AATCCACCCT GTTTGCTGTC CACTGTTCAG TACATCAGTA GCTTTTATGC CAGCTGTCTG
    TCTGGAGAGG AGTCCTATTG GTGGATGCAG TTCACGGCGG CCGTGGAATT CATTAAGACT
    ATCGATGACC GAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220566
    Clone ID Related Accession (Same CDS sequence) XM_024999022.1
    Accession Version XM_024999022.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4383bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGCT CCTATACCAT TTAGAAAGAA AGAAAAACAA
    GAAAAAGACA AAGATGATCT GGGGCCTGAC AGATTCTCAA CACTCACAGA TGATCCCAGC
    CCGAGACTCA GTGCACAGGC TCAGGTCGCT GAGGATATTC TGGACAAATA CAGGAATGCC
    ATCAAACGCA CCAGCCCCAG TGAGGGAGCT CTGGCAAACT ACGAAAGTGC AGTCATGGGT
    GACGGTGAAA GCGCACACGA CTCTCCTCGT GACGAAACCC TGCAGAACAT CTCCGCTGAC
    GATCTCCCAG ATTCTGCGAG CCAAGCGGCT CACCCTCAGG ATTCAGCCTT CTCTTACAGA
    GATGCAAAAA AGAAACTGAG GCTCGCTCTT TGCTCTGCGG ATTCTGTGGC TTTCCCAGTG
    CTAACCCACT CAACAAGAAA CGGTTTACCA GACCATACAG ACCCAGAAGA CAATGAAATT
    GTCTGCTTCT TAAAAGTGCA AATAGCTGAA GCAATTAACT TACAAGATAA GAACTTAATG
    GCTCAGCTTC AGGAAACAAT GCGTTGTGTG TGCCGCTTTG ATAACCGGAC CTGTAGGAAG
    CTGCTGGCGT CCATCGCCGA GGACTACAGG AAGCGGGCCC CCTACATTGC TTACCTCACT
    CGCTGTCGAC AAGGACTGCA GACCACGCAG GCTCACCTGG AAAGGCTACT GCAGAGGGTT
    CTGCGGGACA AAGAGGTGGC CAATCGATAC TTCACCACCG TCTGTGTGAG GCTGCTGCTT
    GAGAGCAAAG AGAAGAAGAT CAGGGAATTC ATTCAAGACT TTCAGAAACT CACAGCCGCT
    GATGATAAAA CCGCCCAAGT GGAAGATTTT CTGCAGTTCC TGTACGGCGC GATGGCCCAG
    GACGTCATAT GGCAGAACGC GAGTGAGGAA CAGCTTCAGG ACGCTCAGCT GGCCATTGAG
    AGAAGTGTGA TGAACCGGAT CTTCAAACTT GCCTTCTACC CAAATCAGGA CGGGGACATA
    CTCCGTGACC AGGTTCTTCA TGAACATATT CAGAGATTGT CTAAAGTAGT GACTGCAAAT
    CACAGAGCTC TGCAGATACC AGAGGTTTAT CTTAAGGAGG CACCGTGGCC CTCGGCACAG
    TCAGAAATCA GGACAATAAG TGCTTACAAG ACGCCCCGGG ACAAAGTGCA GTGCATCCTG
    AGAATGTGCT CCACGATCAT GAACCTCCTG AGCCTGGCCA ACGAGGACTC CGTCCCAGGG
    GCAGATGACT TCGTCCCTGT GCTGGTGTTT GTGTTGATCA AGGCAAATCC ACCCTGTTTG
    CTGTCCACTG TTCAGTACAT CAGTAGCTTT TATGCCAGCT GTCTGTCTGG AGAGGAGTCC
    TATTGGTGGA TGCAGTTCAC GGCGGCCGTG GAATTCATTA AGACTATCGA TGACCGAAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854790.1
    CDS184..4566
    Translation

    Target ORF information:

    RefSeq Version XM_024999022.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999022.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGCT CCTATACCAT TTAGAAAGAA AGAAAAACAA
    GAAAAAGACA AAGATGATCT GGGGCCTGAC AGATTCTCAA CACTCACAGA TGATCCCAGC
    CCGAGACTCA GTGCACAGGC TCAGGTCGCT GAGGATATTC TGGACAAATA CAGGAATGCC
    ATCAAACGCA CCAGCCCCAG TGAGGGAGCT CTGGCAAACT ACGAAAGTGC AGTCATGGGT
    GACGGTGAAA GCGCACACGA CTCTCCTCGT GACGAAACCC TGCAGAACAT CTCCGCTGAC
    GATCTCCCAG ATTCTGCGAG CCAAGCGGCT CACCCTCAGG ATTCAGCCTT CTCTTACAGA
    GATGCAAAAA AGAAACTGAG GCTCGCTCTT TGCTCTGCGG ATTCTGTGGC TTTCCCAGTG
    CTAACCCACT CAACAAGAAA CGGTTTACCA GACCATACAG ACCCAGAAGA CAATGAAATT
    GTCTGCTTCT TAAAAGTGCA AATAGCTGAA GCAATTAACT TACAAGATAA GAACTTAATG
    GCTCAGCTTC AGGAAACAAT GCGTTGTGTG TGCCGCTTTG ATAACCGGAC CTGTAGGAAG
    CTGCTGGCGT CCATCGCCGA GGACTACAGG AAGCGGGCCC CCTACATTGC TTACCTCACT
    CGCTGTCGAC AAGGACTGCA GACCACGCAG GCTCACCTGG AAAGGCTACT GCAGAGGGTT
    CTGCGGGACA AAGAGGTGGC CAATCGATAC TTCACCACCG TCTGTGTGAG GCTGCTGCTT
    GAGAGCAAAG AGAAGAAGAT CAGGGAATTC ATTCAAGACT TTCAGAAACT CACAGCCGCT
    GATGATAAAA CCGCCCAAGT GGAAGATTTT CTGCAGTTCC TGTACGGCGC GATGGCCCAG
    GACGTCATAT GGCAGAACGC GAGTGAGGAA CAGCTTCAGG ACGCTCAGCT GGCCATTGAG
    AGAAGTGTGA TGAACCGGAT CTTCAAACTT GCCTTCTACC CAAATCAGGA CGGGGACATA
    CTCCGTGACC AGGTTCTTCA TGAACATATT CAGAGATTGT CTAAAGTAGT GACTGCAAAT
    CACAGAGCTC TGCAGATACC AGAGGTTTAT CTTAAGGAGG CACCGTGGCC CTCGGCACAG
    TCAGAAATCA GGACAATAAG TGCTTACAAG ACGCCCCGGG ACAAAGTGCA GTGCATCCTG
    AGAATGTGCT CCACGATCAT GAACCTCCTG AGCCTGGCCA ACGAGGACTC CGTCCCAGGG
    GCAGATGACT TCGTCCCTGT GCTGGTGTTT GTGTTGATCA AGGCAAATCC ACCCTGTTTG
    CTGTCCACTG TTCAGTACAT CAGTAGCTTT TATGCCAGCT GTCTGTCTGG AGAGGAGTCC
    TATTGGTGGA TGCAGTTCAC GGCGGCCGTG GAATTCATTA AGACTATCGA TGACCGAAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa44066
    Clone ID Related Accession (Same CDS sequence) NM_001102520.1
    Accession Version NM_001102520.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Bos taurus(cattle)
    Product GTPase-activating protein and VPS9 domain-containing protein 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC140474.1. On Aug 29, 2007 this sequence version replaced XR_027782.1. ##Evidence-Data-START## Transcript exon combination :: BC140474.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGATCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TCCTTCAAAT
    CGTTCTAATT CAGTGTCCTC ACTAGACCTG GAGGGGGAAT CTGTGTCAGA GCTTGGAGCA
    GGACCTTCTG GGAGTAATGG AGTTGAAGCT CTACAGCTGT TAGAGCATGA GCAAGCTACC
    ACGCAGGATA ATCTTGATGA TAAGCTCCGG AAGTTTGAAA TCCGTGACAT GATGGGACTG
    ACGGATGACA GGGATATATC AGAAACAGTG AGTGAGACGT GGAGTACGGA TGTCTTGGGA
    AGTGATTTTG ACCCTAACAT TGACGAAGAT CGCTTGCAAG AAATCGCAGG TGCAGCAGCA
    GAGAACATGT TAGGCAGTTT ACTGTGCCTG CCAGGTTCGG GGTCAGTGCT TCTTGACCCC
    TGCACTGGTT CCACCATATC AGAGACAACA AGCGAAGCTT GGAGTGTAGA GGTATTGCCA
    AGTGACTCAG AGGCCCCAGA TCTAAAGCAG GAAGAGCGAC TGCAGGAACT GGAGAGCTGT
    TCTGGACTGG GTAGCACATC TGATGATACG GATGTCAGGG AGGTCAGTTC CCGCCCCAGC
    ACACCAGGCC TCAGTGTTGT GTCGGGCATA AGTGCAACCT CTGAGGATAT TCCCAATAAG
    ATTGAAGATC TGAGATCTGA GTGCAGCTCT GATTTTGGGG GTAAAGATTC TGTCACTAGT
    CCAGACATGG ATGAAGTCAC CCATGGTGCC CACCAGCTGA CCTCTCCTCC GTCTCAGTCA
    GAGTCTCTGC TTGCCATGTT TGATCCATTG TCTTCGCACG AAGGGGGAGC TTCTGCTGTG
    GTAAGGCCGA AGGTTCACTA TGCCCGGCCG TCGCACCCAC CCCCAGACCC CCCGATCCTG
    GAAGGAGCTG TGGGAGGAAA CGAGGCCAGG TTGCCTACCT TCGGTTCCCA TATCCTGACT
    CCAGCTGAGA TGGAGGCGTT TAAGCAAAGG CACTCGTACC CCGAGAGATT AGTTCGCAGC
    AGGAGCTCCG ACGTCGTGTC TTCTGTCCGG CGCCCGATGA GCGACCCCAG CTGGAACCGA
    CGCCCAGGGA ATGAAGAGCG AGAACTCCCT CCAGCTGCAG CCATTGGTGC TACTTCTTTG
    GTGGCTGCGC CTCACTCTTC ATCTTCATCC CCGAGTAAGG ACTCCTCAAG AGGAGAGACT
    GAAGAACGCA AAGATAGCGA TGATGAGAAA TCAGACAGGA ACAGACCTTG GTGGAGAAAA
    CGTTTTGTTT CTGCCATGCC CAAAGATGAT CCCAGCCCGA GACTCAGTGC ACAGGCTCAG
    GTCGCTGAGG ATATTCTGGA CAAATACAGG AATGCCATCA AACGCACCAG CCCCAGTGAG
    GGAGCTCTGG CAAACTACGA AAGTGCAGAA GTCATGGGTG ACGGTGAAAG CGCACACGAC
    TCTCCTCGTG ACGAAACCCT GCAGAACATC TCCGCTGACG ATCTCCCAGA TTCTGCGAGC
    CAAGCGGCTC ACCCTCAGGA TTCAGCCTTC TCTTACAGAG ATGCAAAAAA GAAACTGAGG
    CTCGCTCTTT GCTCTGCGGA TTCTGTGGCT TTCCCAGTGC TAACCCACTC AACAAGAAAC
    GGTTTACCAG ACCATACAGA CCCAGAAGAC AATGAAATTG TCTGCTTCTT AAAAGTGCAA
    ATAGCTGAAG CAATTAACTT ACAAGATAAG AACTTAATGG CTCAGCTTCA GGAAACAATG
    CGTTGTGTGT GCCGCTTTGA TAACCGGACC TGTAGGAAGC TGCTGGCGTC CATCGCCGAG
    GACTACAGGA AGCGGGCCCC CTACATTGCT TACCTCACTC GCTGTCGACA AGGACTGCAG
    ACCACGCAGG CTCACCTGGA AAGGCTACTG CAGAGGGTTC TGCGGGACAA AGAGGTGGCC
    AATCGATACT TCACCACCGT CTGTGTGAGG CTGCTGCTTG AGAGCAAAGA GAAGAAGATC
    AGGGAATTCA TTCAAGACTT TCAGAAACTC ACAGCCGCTG ATGATAAAAC CGCCCAAGTG
    GAAGATTTTC TGCAGTTCCT GTACGGCGCG ATGGCCCAGG ACGTCATATG GCAGAACGCG
    AGTGAGGAAC AGCTTCAGGA CGCTCAGCTG GCCATTGAGA GAAGTGTGAT GAACCGGATC
    TTCAAACTTG CCTTCTACCC AAATCAGGAC GGGGACATAC TCCGTGACCA GGTTCTTCAT
    GAACATATTC AGAGATTGTC TAAAGTAGTG ACTGCAAATC ACAGAGCTCT GCAGATACCA
    GAGGTTTATC TTAAGGAGGC ACCGTGGCCC TCGGCACAGT CAGAAATCAG GACAATAAGT
    GCTTACAAGA CGCCCCGGGA CAAAGTGCAG TGCATCCTGA GAATGTGCTC CACGATCATG
    AACCTCCTGA GCCTGGCCAA CGAGGACTCC GTCCCAGGGG CAGATGACTT CGTCCCTGTG
    CTGGTGTTTG TGTTGATCAA GGCAAATCCA CCCTGTTTGC TGTCCACTGT TCAGTACATC
    AGTAGCTTTT ATGCCAGCTG TCTGTCTGGA GAGGAGTCCT ATTGGTGGAT GCAGTTCACG
    GCGGCCGTGG AATTCATTAA GACTATCGAT GACCGAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001095990.1
    CDS152..4393
    Translation

    Target ORF information:

    RefSeq Version NM_001102520.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001102520.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGATCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TCCTTCAAAT
    CGTTCTAATT CAGTGTCCTC ACTAGACCTG GAGGGGGAAT CTGTGTCAGA GCTTGGAGCA
    GGACCTTCTG GGAGTAATGG AGTTGAAGCT CTACAGCTGT TAGAGCATGA GCAAGCTACC
    ACGCAGGATA ATCTTGATGA TAAGCTCCGG AAGTTTGAAA TCCGTGACAT GATGGGACTG
    ACGGATGACA GGGATATATC AGAAACAGTG AGTGAGACGT GGAGTACGGA TGTCTTGGGA
    AGTGATTTTG ACCCTAACAT TGACGAAGAT CGCTTGCAAG AAATCGCAGG TGCAGCAGCA
    GAGAACATGT TAGGCAGTTT ACTGTGCCTG CCAGGTTCGG GGTCAGTGCT TCTTGACCCC
    TGCACTGGTT CCACCATATC AGAGACAACA AGCGAAGCTT GGAGTGTAGA GGTATTGCCA
    AGTGACTCAG AGGCCCCAGA TCTAAAGCAG GAAGAGCGAC TGCAGGAACT GGAGAGCTGT
    TCTGGACTGG GTAGCACATC TGATGATACG GATGTCAGGG AGGTCAGTTC CCGCCCCAGC
    ACACCAGGCC TCAGTGTTGT GTCGGGCATA AGTGCAACCT CTGAGGATAT TCCCAATAAG
    ATTGAAGATC TGAGATCTGA GTGCAGCTCT GATTTTGGGG GTAAAGATTC TGTCACTAGT
    CCAGACATGG ATGAAGTCAC CCATGGTGCC CACCAGCTGA CCTCTCCTCC GTCTCAGTCA
    GAGTCTCTGC TTGCCATGTT TGATCCATTG TCTTCGCACG AAGGGGGAGC TTCTGCTGTG
    GTAAGGCCGA AGGTTCACTA TGCCCGGCCG TCGCACCCAC CCCCAGACCC CCCGATCCTG
    GAAGGAGCTG TGGGAGGAAA CGAGGCCAGG TTGCCTACCT TCGGTTCCCA TATCCTGACT
    CCAGCTGAGA TGGAGGCGTT TAAGCAAAGG CACTCGTACC CCGAGAGATT AGTTCGCAGC
    AGGAGCTCCG ACGTCGTGTC TTCTGTCCGG CGCCCGATGA GCGACCCCAG CTGGAACCGA
    CGCCCAGGGA ATGAAGAGCG AGAACTCCCT CCAGCTGCAG CCATTGGTGC TACTTCTTTG
    GTGGCTGCGC CTCACTCTTC ATCTTCATCC CCGAGTAAGG ACTCCTCAAG AGGAGAGACT
    GAAGAACGCA AAGATAGCGA TGATGAGAAA TCAGACAGGA ACAGACCTTG GTGGAGAAAA
    CGTTTTGTTT CTGCCATGCC CAAAGATGAT CCCAGCCCGA GACTCAGTGC ACAGGCTCAG
    GTCGCTGAGG ATATTCTGGA CAAATACAGG AATGCCATCA AACGCACCAG CCCCAGTGAG
    GGAGCTCTGG CAAACTACGA AAGTGCAGAA GTCATGGGTG ACGGTGAAAG CGCACACGAC
    TCTCCTCGTG ACGAAACCCT GCAGAACATC TCCGCTGACG ATCTCCCAGA TTCTGCGAGC
    CAAGCGGCTC ACCCTCAGGA TTCAGCCTTC TCTTACAGAG ATGCAAAAAA GAAACTGAGG
    CTCGCTCTTT GCTCTGCGGA TTCTGTGGCT TTCCCAGTGC TAACCCACTC AACAAGAAAC
    GGTTTACCAG ACCATACAGA CCCAGAAGAC AATGAAATTG TCTGCTTCTT AAAAGTGCAA
    ATAGCTGAAG CAATTAACTT ACAAGATAAG AACTTAATGG CTCAGCTTCA GGAAACAATG
    CGTTGTGTGT GCCGCTTTGA TAACCGGACC TGTAGGAAGC TGCTGGCGTC CATCGCCGAG
    GACTACAGGA AGCGGGCCCC CTACATTGCT TACCTCACTC GCTGTCGACA AGGACTGCAG
    ACCACGCAGG CTCACCTGGA AAGGCTACTG CAGAGGGTTC TGCGGGACAA AGAGGTGGCC
    AATCGATACT TCACCACCGT CTGTGTGAGG CTGCTGCTTG AGAGCAAAGA GAAGAAGATC
    AGGGAATTCA TTCAAGACTT TCAGAAACTC ACAGCCGCTG ATGATAAAAC CGCCCAAGTG
    GAAGATTTTC TGCAGTTCCT GTACGGCGCG ATGGCCCAGG ACGTCATATG GCAGAACGCG
    AGTGAGGAAC AGCTTCAGGA CGCTCAGCTG GCCATTGAGA GAAGTGTGAT GAACCGGATC
    TTCAAACTTG CCTTCTACCC AAATCAGGAC GGGGACATAC TCCGTGACCA GGTTCTTCAT
    GAACATATTC AGAGATTGTC TAAAGTAGTG ACTGCAAATC ACAGAGCTCT GCAGATACCA
    GAGGTTTATC TTAAGGAGGC ACCGTGGCCC TCGGCACAGT CAGAAATCAG GACAATAAGT
    GCTTACAAGA CGCCCCGGGA CAAAGTGCAG TGCATCCTGA GAATGTGCTC CACGATCATG
    AACCTCCTGA GCCTGGCCAA CGAGGACTCC GTCCCAGGGG CAGATGACTT CGTCCCTGTG
    CTGGTGTTTG TGTTGATCAA GGCAAATCCA CCCTGTTTGC TGTCCACTGT TCAGTACATC
    AGTAGCTTTT ATGCCAGCTG TCTGTCTGGA GAGGAGTCCT ATTGGTGGAT GCAGTTCACG
    GCGGCCGTGG AATTCATTAA GACTATCGAT GACCGAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54402
    Clone ID Related Accession (Same CDS sequence) XM_010810351.2 , XM_010810351.3
    Accession Version XM_010810351.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104301.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810351.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGCT CCTATACCAT TTAGAAAGAA AGAAAAACAA
    GAAAAAGACA AAGATGATCT GGGGCCTGAC AGATTCTCAA CACTCACAGA TGATCCCAGC
    CCGAGACTCA GTGCACAGGC TCAGGTCGCT GAGGATATTC TGGACAAATA CAGGAATGCC
    ATCAAACGCA CCAGCCCCAG TGAGGGAGCT CTGGCAAACT ACGAAAGTGC AGAAGTCATG
    GGTGACGGTG AAAGCGCACA CGACTCTCCT CGTGACGAAA CCCTGCAGAA CATCTCCGCT
    GACGATCTCC CAGATTCTGC GAGCCAAGCG GCTCACCCTC AGGATTCAGC CTTCTCTTAC
    AGAGATGCAA AAAAGAAACT GAGGCTCGCT CTTTGCTCTG CGGATTCTGT GGCTTTCCCA
    GTGCTAACCC ACTCAACAAG AAACGGTTTA CCAGACCATA CAGACCCAGA AGACAATGAA
    ATTGTCTGCT TCTTAAAAGT GCAAATAGCT GAAGCAATTA ACTTACAAGA TAAGAACTTA
    ATGGCTCAGC TTCAGGAAAC AATGCGTTGT GTGTGCCGCT TTGATAACCG GACCTGTAGG
    AAGCTGCTGG CGTCCATCGC CGAGGACTAC AGCGTCTTTC CTTTCAGGAA GCGGGCCCCC
    TACATTGCTT ACCTCACTCG CTGTCGACAA GGACTGCAGA CCACGCAGGC TCACCTGGAA
    AGGCTACTGC AGAGGGTTCT GCGGGACAAA GAGGTGGCCA ATCGATACTT CACCACCGTC
    TGTGTGAGGC TGCTGCTTGA GAGCAAAGAG AAGAAGATCA GGGAATTCAT TCAAGACTTT
    CAGAAACTCA CAGCCGCTGA TGATAAAACC GCCCAAGTGG AAGATTTTCT GCAGTTCCTG
    TACGGCGCGA TGGCCCAGGA CGTCATATGG CAGAACGCGA GTGAGGAACA GCTTCAGGAC
    GCTCAGCTGG CCATTGAGAG AAGTGTGATG AACCGGATCT TCAAACTTGC CTTCTACCCA
    AATCAGGACG GGGACATACT CCGTGACCAG GTTCTTCATG AACATATTCA GAGATTGTCT
    AAAGTAGTGA CTGCAAATCA CAGAGCTCTG CAGATACCAG AGGTTTATCT TAAGGAGGCA
    CCGTGGCCCT CGGCACAGTC AGAAATCAGG ACAATAAGTG CTTACAAGAC GCCCCGGGAC
    AAAGTGCAGT GCATCCTGAG AATGTGCTCC ACGATCATGA ACCTCCTGAG CCTGGCCAAC
    GAGGACTCCG TCCCAGGGGC AGATGACTTC GTCCCTGTGC TGGTGTTTGT GTTGATCAAG
    GCAAATCCAC CCTGTTTGCT GTCCACTGTT CAGTACATCA GTAGCTTTTA TGCCAGCTGT
    CTGTCTGGAG AGGAGTCCTA TTGGTGGATG CAGTTCACGG CGGCCGTGGA ATTCATTAAG
    ACTATCGATG ACCGAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808653.1
    CDS174..4574
    Translation

    Target ORF information:

    RefSeq Version XM_010810351.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810351.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGCT CCTATACCAT TTAGAAAGAA AGAAAAACAA
    GAAAAAGACA AAGATGATCT GGGGCCTGAC AGATTCTCAA CACTCACAGA TGATCCCAGC
    CCGAGACTCA GTGCACAGGC TCAGGTCGCT GAGGATATTC TGGACAAATA CAGGAATGCC
    ATCAAACGCA CCAGCCCCAG TGAGGGAGCT CTGGCAAACT ACGAAAGTGC AGAAGTCATG
    GGTGACGGTG AAAGCGCACA CGACTCTCCT CGTGACGAAA CCCTGCAGAA CATCTCCGCT
    GACGATCTCC CAGATTCTGC GAGCCAAGCG GCTCACCCTC AGGATTCAGC CTTCTCTTAC
    AGAGATGCAA AAAAGAAACT GAGGCTCGCT CTTTGCTCTG CGGATTCTGT GGCTTTCCCA
    GTGCTAACCC ACTCAACAAG AAACGGTTTA CCAGACCATA CAGACCCAGA AGACAATGAA
    ATTGTCTGCT TCTTAAAAGT GCAAATAGCT GAAGCAATTA ACTTACAAGA TAAGAACTTA
    ATGGCTCAGC TTCAGGAAAC AATGCGTTGT GTGTGCCGCT TTGATAACCG GACCTGTAGG
    AAGCTGCTGG CGTCCATCGC CGAGGACTAC AGCGTCTTTC CTTTCAGGAA GCGGGCCCCC
    TACATTGCTT ACCTCACTCG CTGTCGACAA GGACTGCAGA CCACGCAGGC TCACCTGGAA
    AGGCTACTGC AGAGGGTTCT GCGGGACAAA GAGGTGGCCA ATCGATACTT CACCACCGTC
    TGTGTGAGGC TGCTGCTTGA GAGCAAAGAG AAGAAGATCA GGGAATTCAT TCAAGACTTT
    CAGAAACTCA CAGCCGCTGA TGATAAAACC GCCCAAGTGG AAGATTTTCT GCAGTTCCTG
    TACGGCGCGA TGGCCCAGGA CGTCATATGG CAGAACGCGA GTGAGGAACA GCTTCAGGAC
    GCTCAGCTGG CCATTGAGAG AAGTGTGATG AACCGGATCT TCAAACTTGC CTTCTACCCA
    AATCAGGACG GGGACATACT CCGTGACCAG GTTCTTCATG AACATATTCA GAGATTGTCT
    AAAGTAGTGA CTGCAAATCA CAGAGCTCTG CAGATACCAG AGGTTTATCT TAAGGAGGCA
    CCGTGGCCCT CGGCACAGTC AGAAATCAGG ACAATAAGTG CTTACAAGAC GCCCCGGGAC
    AAAGTGCAGT GCATCCTGAG AATGTGCTCC ACGATCATGA ACCTCCTGAG CCTGGCCAAC
    GAGGACTCCG TCCCAGGGGC AGATGACTTC GTCCCTGTGC TGGTGTTTGT GTTGATCAAG
    GCAAATCCAC CCTGTTTGCT GTCCACTGTT CAGTACATCA GTAGCTTTTA TGCCAGCTGT
    CTGTCTGGAG AGGAGTCCTA TTGGTGGATG CAGTTCACGG CGGCCGTGGA ATTCATTAAG
    ACTATCGATG ACCGAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54402
    Clone ID Related Accession (Same CDS sequence) XM_010810351.2 , XM_010810351.3
    Accession Version XM_010810351.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810351.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGCT CCTATACCAT TTAGAAAGAA AGAAAAACAA
    GAAAAAGACA AAGATGATCT GGGGCCTGAC AGATTCTCAA CACTCACAGA TGATCCCAGC
    CCGAGACTCA GTGCACAGGC TCAGGTCGCT GAGGATATTC TGGACAAATA CAGGAATGCC
    ATCAAACGCA CCAGCCCCAG TGAGGGAGCT CTGGCAAACT ACGAAAGTGC AGAAGTCATG
    GGTGACGGTG AAAGCGCACA CGACTCTCCT CGTGACGAAA CCCTGCAGAA CATCTCCGCT
    GACGATCTCC CAGATTCTGC GAGCCAAGCG GCTCACCCTC AGGATTCAGC CTTCTCTTAC
    AGAGATGCAA AAAAGAAACT GAGGCTCGCT CTTTGCTCTG CGGATTCTGT GGCTTTCCCA
    GTGCTAACCC ACTCAACAAG AAACGGTTTA CCAGACCATA CAGACCCAGA AGACAATGAA
    ATTGTCTGCT TCTTAAAAGT GCAAATAGCT GAAGCAATTA ACTTACAAGA TAAGAACTTA
    ATGGCTCAGC TTCAGGAAAC AATGCGTTGT GTGTGCCGCT TTGATAACCG GACCTGTAGG
    AAGCTGCTGG CGTCCATCGC CGAGGACTAC AGCGTCTTTC CTTTCAGGAA GCGGGCCCCC
    TACATTGCTT ACCTCACTCG CTGTCGACAA GGACTGCAGA CCACGCAGGC TCACCTGGAA
    AGGCTACTGC AGAGGGTTCT GCGGGACAAA GAGGTGGCCA ATCGATACTT CACCACCGTC
    TGTGTGAGGC TGCTGCTTGA GAGCAAAGAG AAGAAGATCA GGGAATTCAT TCAAGACTTT
    CAGAAACTCA CAGCCGCTGA TGATAAAACC GCCCAAGTGG AAGATTTTCT GCAGTTCCTG
    TACGGCGCGA TGGCCCAGGA CGTCATATGG CAGAACGCGA GTGAGGAACA GCTTCAGGAC
    GCTCAGCTGG CCATTGAGAG AAGTGTGATG AACCGGATCT TCAAACTTGC CTTCTACCCA
    AATCAGGACG GGGACATACT CCGTGACCAG GTTCTTCATG AACATATTCA GAGATTGTCT
    AAAGTAGTGA CTGCAAATCA CAGAGCTCTG CAGATACCAG AGGTTTATCT TAAGGAGGCA
    CCGTGGCCCT CGGCACAGTC AGAAATCAGG ACAATAAGTG CTTACAAGAC GCCCCGGGAC
    AAAGTGCAGT GCATCCTGAG AATGTGCTCC ACGATCATGA ACCTCCTGAG CCTGGCCAAC
    GAGGACTCCG TCCCAGGGGC AGATGACTTC GTCCCTGTGC TGGTGTTTGT GTTGATCAAG
    GCAAATCCAC CCTGTTTGCT GTCCACTGTT CAGTACATCA GTAGCTTTTA TGCCAGCTGT
    CTGTCTGGAG AGGAGTCCTA TTGGTGGATG CAGTTCACGG CGGCCGTGGA ATTCATTAAG
    ACTATCGATG ACCGAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808653.1
    CDS185..4585
    Translation

    Target ORF information:

    RefSeq Version XM_010810351.3
    Organism Bos taurus(cattle)
    Definition Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810351.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGCT CCTATACCAT TTAGAAAGAA AGAAAAACAA
    GAAAAAGACA AAGATGATCT GGGGCCTGAC AGATTCTCAA CACTCACAGA TGATCCCAGC
    CCGAGACTCA GTGCACAGGC TCAGGTCGCT GAGGATATTC TGGACAAATA CAGGAATGCC
    ATCAAACGCA CCAGCCCCAG TGAGGGAGCT CTGGCAAACT ACGAAAGTGC AGAAGTCATG
    GGTGACGGTG AAAGCGCACA CGACTCTCCT CGTGACGAAA CCCTGCAGAA CATCTCCGCT
    GACGATCTCC CAGATTCTGC GAGCCAAGCG GCTCACCCTC AGGATTCAGC CTTCTCTTAC
    AGAGATGCAA AAAAGAAACT GAGGCTCGCT CTTTGCTCTG CGGATTCTGT GGCTTTCCCA
    GTGCTAACCC ACTCAACAAG AAACGGTTTA CCAGACCATA CAGACCCAGA AGACAATGAA
    ATTGTCTGCT TCTTAAAAGT GCAAATAGCT GAAGCAATTA ACTTACAAGA TAAGAACTTA
    ATGGCTCAGC TTCAGGAAAC AATGCGTTGT GTGTGCCGCT TTGATAACCG GACCTGTAGG
    AAGCTGCTGG CGTCCATCGC CGAGGACTAC AGCGTCTTTC CTTTCAGGAA GCGGGCCCCC
    TACATTGCTT ACCTCACTCG CTGTCGACAA GGACTGCAGA CCACGCAGGC TCACCTGGAA
    AGGCTACTGC AGAGGGTTCT GCGGGACAAA GAGGTGGCCA ATCGATACTT CACCACCGTC
    TGTGTGAGGC TGCTGCTTGA GAGCAAAGAG AAGAAGATCA GGGAATTCAT TCAAGACTTT
    CAGAAACTCA CAGCCGCTGA TGATAAAACC GCCCAAGTGG AAGATTTTCT GCAGTTCCTG
    TACGGCGCGA TGGCCCAGGA CGTCATATGG CAGAACGCGA GTGAGGAACA GCTTCAGGAC
    GCTCAGCTGG CCATTGAGAG AAGTGTGATG AACCGGATCT TCAAACTTGC CTTCTACCCA
    AATCAGGACG GGGACATACT CCGTGACCAG GTTCTTCATG AACATATTCA GAGATTGTCT
    AAAGTAGTGA CTGCAAATCA CAGAGCTCTG CAGATACCAG AGGTTTATCT TAAGGAGGCA
    CCGTGGCCCT CGGCACAGTC AGAAATCAGG ACAATAAGTG CTTACAAGAC GCCCCGGGAC
    AAAGTGCAGT GCATCCTGAG AATGTGCTCC ACGATCATGA ACCTCCTGAG CCTGGCCAAC
    GAGGACTCCG TCCCAGGGGC AGATGACTTC GTCCCTGTGC TGGTGTTTGT GTTGATCAAG
    GCAAATCCAC CCTGTTTGCT GTCCACTGTT CAGTACATCA GTAGCTTTTA TGCCAGCTGT
    CTGTCTGGAG AGGAGTCCTA TTGGTGGATG CAGTTCACGG CGGCCGTGGA ATTCATTAAG
    ACTATCGATG ACCGAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54403
    Clone ID Related Accession (Same CDS sequence) XM_010810352.3 , XM_010810352.2
    Accession Version XM_010810352.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4386bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810352.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGCT CCTATACCAT TTAGAAAGAA AGAAAAACAA
    GAAAAAGACA AAGATGATCT GGGGCCTGAC AGATTCTCAA CACTCACAGA TGATCCCAGC
    CCGAGACTCA GTGCACAGGC TCAGGTCGCT GAGGATATTC TGGACAAATA CAGGAATGCC
    ATCAAACGCA CCAGCCCCAG TGAGGGAGCT CTGGCAAACT ACGAAAGTGC AGAAGTCATG
    GGTGACGGTG AAAGCGCACA CGACTCTCCT CGTGACGAAA CCCTGCAGAA CATCTCCGCT
    GACGATCTCC CAGATTCTGC GAGCCAAGCG GCTCACCCTC AGGATTCAGC CTTCTCTTAC
    AGAGATGCAA AAAAGAAACT GAGGCTCGCT CTTTGCTCTG CGGATTCTGT GGCTTTCCCA
    GTGCTAACCC ACTCAACAAG AAACGGTTTA CCAGACCATA CAGACCCAGA AGACAATGAA
    ATTGTCTGCT TCTTAAAAGT GCAAATAGCT GAAGCAATTA ACTTACAAGA TAAGAACTTA
    ATGGCTCAGC TTCAGGAAAC AATGCGTTGT GTGTGCCGCT TTGATAACCG GACCTGTAGG
    AAGCTGCTGG CGTCCATCGC CGAGGACTAC AGGAAGCGGG CCCCCTACAT TGCTTACCTC
    ACTCGCTGTC GACAAGGACT GCAGACCACG CAGGCTCACC TGGAAAGGCT ACTGCAGAGG
    GTTCTGCGGG ACAAAGAGGT GGCCAATCGA TACTTCACCA CCGTCTGTGT GAGGCTGCTG
    CTTGAGAGCA AAGAGAAGAA GATCAGGGAA TTCATTCAAG ACTTTCAGAA ACTCACAGCC
    GCTGATGATA AAACCGCCCA AGTGGAAGAT TTTCTGCAGT TCCTGTACGG CGCGATGGCC
    CAGGACGTCA TATGGCAGAA CGCGAGTGAG GAACAGCTTC AGGACGCTCA GCTGGCCATT
    GAGAGAAGTG TGATGAACCG GATCTTCAAA CTTGCCTTCT ACCCAAATCA GGACGGGGAC
    ATACTCCGTG ACCAGGTTCT TCATGAACAT ATTCAGAGAT TGTCTAAAGT AGTGACTGCA
    AATCACAGAG CTCTGCAGAT ACCAGAGGTT TATCTTAAGG AGGCACCGTG GCCCTCGGCA
    CAGTCAGAAA TCAGGACAAT AAGTGCTTAC AAGACGCCCC GGGACAAAGT GCAGTGCATC
    CTGAGAATGT GCTCCACGAT CATGAACCTC CTGAGCCTGG CCAACGAGGA CTCCGTCCCA
    GGGGCAGATG ACTTCGTCCC TGTGCTGGTG TTTGTGTTGA TCAAGGCAAA TCCACCCTGT
    TTGCTGTCCA CTGTTCAGTA CATCAGTAGC TTTTATGCCA GCTGTCTGTC TGGAGAGGAG
    TCCTATTGGT GGATGCAGTT CACGGCGGCC GTGGAATTCA TTAAGACTAT CGATGACCGA
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808654.1
    CDS170..4555
    Translation

    Target ORF information:

    RefSeq Version XM_010810352.3
    Organism Bos taurus(cattle)
    Definition Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810352.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGCT CCTATACCAT TTAGAAAGAA AGAAAAACAA
    GAAAAAGACA AAGATGATCT GGGGCCTGAC AGATTCTCAA CACTCACAGA TGATCCCAGC
    CCGAGACTCA GTGCACAGGC TCAGGTCGCT GAGGATATTC TGGACAAATA CAGGAATGCC
    ATCAAACGCA CCAGCCCCAG TGAGGGAGCT CTGGCAAACT ACGAAAGTGC AGAAGTCATG
    GGTGACGGTG AAAGCGCACA CGACTCTCCT CGTGACGAAA CCCTGCAGAA CATCTCCGCT
    GACGATCTCC CAGATTCTGC GAGCCAAGCG GCTCACCCTC AGGATTCAGC CTTCTCTTAC
    AGAGATGCAA AAAAGAAACT GAGGCTCGCT CTTTGCTCTG CGGATTCTGT GGCTTTCCCA
    GTGCTAACCC ACTCAACAAG AAACGGTTTA CCAGACCATA CAGACCCAGA AGACAATGAA
    ATTGTCTGCT TCTTAAAAGT GCAAATAGCT GAAGCAATTA ACTTACAAGA TAAGAACTTA
    ATGGCTCAGC TTCAGGAAAC AATGCGTTGT GTGTGCCGCT TTGATAACCG GACCTGTAGG
    AAGCTGCTGG CGTCCATCGC CGAGGACTAC AGGAAGCGGG CCCCCTACAT TGCTTACCTC
    ACTCGCTGTC GACAAGGACT GCAGACCACG CAGGCTCACC TGGAAAGGCT ACTGCAGAGG
    GTTCTGCGGG ACAAAGAGGT GGCCAATCGA TACTTCACCA CCGTCTGTGT GAGGCTGCTG
    CTTGAGAGCA AAGAGAAGAA GATCAGGGAA TTCATTCAAG ACTTTCAGAA ACTCACAGCC
    GCTGATGATA AAACCGCCCA AGTGGAAGAT TTTCTGCAGT TCCTGTACGG CGCGATGGCC
    CAGGACGTCA TATGGCAGAA CGCGAGTGAG GAACAGCTTC AGGACGCTCA GCTGGCCATT
    GAGAGAAGTG TGATGAACCG GATCTTCAAA CTTGCCTTCT ACCCAAATCA GGACGGGGAC
    ATACTCCGTG ACCAGGTTCT TCATGAACAT ATTCAGAGAT TGTCTAAAGT AGTGACTGCA
    AATCACAGAG CTCTGCAGAT ACCAGAGGTT TATCTTAAGG AGGCACCGTG GCCCTCGGCA
    CAGTCAGAAA TCAGGACAAT AAGTGCTTAC AAGACGCCCC GGGACAAAGT GCAGTGCATC
    CTGAGAATGT GCTCCACGAT CATGAACCTC CTGAGCCTGG CCAACGAGGA CTCCGTCCCA
    GGGGCAGATG ACTTCGTCCC TGTGCTGGTG TTTGTGTTGA TCAAGGCAAA TCCACCCTGT
    TTGCTGTCCA CTGTTCAGTA CATCAGTAGC TTTTATGCCA GCTGTCTGTC TGGAGAGGAG
    TCCTATTGGT GGATGCAGTT CACGGCGGCC GTGGAATTCA TTAAGACTAT CGATGACCGA
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54403
    Clone ID Related Accession (Same CDS sequence) XM_010810352.3 , XM_010810352.2
    Accession Version XM_010810352.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4386bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104301.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810352.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGCT CCTATACCAT TTAGAAAGAA AGAAAAACAA
    GAAAAAGACA AAGATGATCT GGGGCCTGAC AGATTCTCAA CACTCACAGA TGATCCCAGC
    CCGAGACTCA GTGCACAGGC TCAGGTCGCT GAGGATATTC TGGACAAATA CAGGAATGCC
    ATCAAACGCA CCAGCCCCAG TGAGGGAGCT CTGGCAAACT ACGAAAGTGC AGAAGTCATG
    GGTGACGGTG AAAGCGCACA CGACTCTCCT CGTGACGAAA CCCTGCAGAA CATCTCCGCT
    GACGATCTCC CAGATTCTGC GAGCCAAGCG GCTCACCCTC AGGATTCAGC CTTCTCTTAC
    AGAGATGCAA AAAAGAAACT GAGGCTCGCT CTTTGCTCTG CGGATTCTGT GGCTTTCCCA
    GTGCTAACCC ACTCAACAAG AAACGGTTTA CCAGACCATA CAGACCCAGA AGACAATGAA
    ATTGTCTGCT TCTTAAAAGT GCAAATAGCT GAAGCAATTA ACTTACAAGA TAAGAACTTA
    ATGGCTCAGC TTCAGGAAAC AATGCGTTGT GTGTGCCGCT TTGATAACCG GACCTGTAGG
    AAGCTGCTGG CGTCCATCGC CGAGGACTAC AGGAAGCGGG CCCCCTACAT TGCTTACCTC
    ACTCGCTGTC GACAAGGACT GCAGACCACG CAGGCTCACC TGGAAAGGCT ACTGCAGAGG
    GTTCTGCGGG ACAAAGAGGT GGCCAATCGA TACTTCACCA CCGTCTGTGT GAGGCTGCTG
    CTTGAGAGCA AAGAGAAGAA GATCAGGGAA TTCATTCAAG ACTTTCAGAA ACTCACAGCC
    GCTGATGATA AAACCGCCCA AGTGGAAGAT TTTCTGCAGT TCCTGTACGG CGCGATGGCC
    CAGGACGTCA TATGGCAGAA CGCGAGTGAG GAACAGCTTC AGGACGCTCA GCTGGCCATT
    GAGAGAAGTG TGATGAACCG GATCTTCAAA CTTGCCTTCT ACCCAAATCA GGACGGGGAC
    ATACTCCGTG ACCAGGTTCT TCATGAACAT ATTCAGAGAT TGTCTAAAGT AGTGACTGCA
    AATCACAGAG CTCTGCAGAT ACCAGAGGTT TATCTTAAGG AGGCACCGTG GCCCTCGGCA
    CAGTCAGAAA TCAGGACAAT AAGTGCTTAC AAGACGCCCC GGGACAAAGT GCAGTGCATC
    CTGAGAATGT GCTCCACGAT CATGAACCTC CTGAGCCTGG CCAACGAGGA CTCCGTCCCA
    GGGGCAGATG ACTTCGTCCC TGTGCTGGTG TTTGTGTTGA TCAAGGCAAA TCCACCCTGT
    TTGCTGTCCA CTGTTCAGTA CATCAGTAGC TTTTATGCCA GCTGTCTGTC TGGAGAGGAG
    TCCTATTGGT GGATGCAGTT CACGGCGGCC GTGGAATTCA TTAAGACTAT CGATGACCGA
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808654.1
    CDS174..4559
    Translation

    Target ORF information:

    RefSeq Version XM_010810352.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810352.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGCT CCTATACCAT TTAGAAAGAA AGAAAAACAA
    GAAAAAGACA AAGATGATCT GGGGCCTGAC AGATTCTCAA CACTCACAGA TGATCCCAGC
    CCGAGACTCA GTGCACAGGC TCAGGTCGCT GAGGATATTC TGGACAAATA CAGGAATGCC
    ATCAAACGCA CCAGCCCCAG TGAGGGAGCT CTGGCAAACT ACGAAAGTGC AGAAGTCATG
    GGTGACGGTG AAAGCGCACA CGACTCTCCT CGTGACGAAA CCCTGCAGAA CATCTCCGCT
    GACGATCTCC CAGATTCTGC GAGCCAAGCG GCTCACCCTC AGGATTCAGC CTTCTCTTAC
    AGAGATGCAA AAAAGAAACT GAGGCTCGCT CTTTGCTCTG CGGATTCTGT GGCTTTCCCA
    GTGCTAACCC ACTCAACAAG AAACGGTTTA CCAGACCATA CAGACCCAGA AGACAATGAA
    ATTGTCTGCT TCTTAAAAGT GCAAATAGCT GAAGCAATTA ACTTACAAGA TAAGAACTTA
    ATGGCTCAGC TTCAGGAAAC AATGCGTTGT GTGTGCCGCT TTGATAACCG GACCTGTAGG
    AAGCTGCTGG CGTCCATCGC CGAGGACTAC AGGAAGCGGG CCCCCTACAT TGCTTACCTC
    ACTCGCTGTC GACAAGGACT GCAGACCACG CAGGCTCACC TGGAAAGGCT ACTGCAGAGG
    GTTCTGCGGG ACAAAGAGGT GGCCAATCGA TACTTCACCA CCGTCTGTGT GAGGCTGCTG
    CTTGAGAGCA AAGAGAAGAA GATCAGGGAA TTCATTCAAG ACTTTCAGAA ACTCACAGCC
    GCTGATGATA AAACCGCCCA AGTGGAAGAT TTTCTGCAGT TCCTGTACGG CGCGATGGCC
    CAGGACGTCA TATGGCAGAA CGCGAGTGAG GAACAGCTTC AGGACGCTCA GCTGGCCATT
    GAGAGAAGTG TGATGAACCG GATCTTCAAA CTTGCCTTCT ACCCAAATCA GGACGGGGAC
    ATACTCCGTG ACCAGGTTCT TCATGAACAT ATTCAGAGAT TGTCTAAAGT AGTGACTGCA
    AATCACAGAG CTCTGCAGAT ACCAGAGGTT TATCTTAAGG AGGCACCGTG GCCCTCGGCA
    CAGTCAGAAA TCAGGACAAT AAGTGCTTAC AAGACGCCCC GGGACAAAGT GCAGTGCATC
    CTGAGAATGT GCTCCACGAT CATGAACCTC CTGAGCCTGG CCAACGAGGA CTCCGTCCCA
    GGGGCAGATG ACTTCGTCCC TGTGCTGGTG TTTGTGTTGA TCAAGGCAAA TCCACCCTGT
    TTGCTGTCCA CTGTTCAGTA CATCAGTAGC TTTTATGCCA GCTGTCTGTC TGGAGAGGAG
    TCCTATTGGT GGATGCAGTT CACGGCGGCC GTGGAATTCA TTAAGACTAT CGATGACCGA
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54404
    Clone ID Related Accession (Same CDS sequence) XM_010810353.2 , XM_010810353.3
    Accession Version XM_010810353.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104301.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TCCTTCAAAT
    CGTTCTAATT CAGTGTCCTC ACTAGACCTG GAGGGGGAAT CTGTGTCAGA GCTTGGAGCA
    GGACCTTCTG GGAGTAATGG AGTTGAAGCT CTACAGCTGT TAGAGCATGA GCAAGCTACC
    ACGCAGGATA ATCTTGATGA TAAGCTCCGG AAGTTTGAAA TCCGTGACAT GATGGGACTG
    ACGGATGACA GGGATATATC AGAAACAGTG AGTGAGACGT GGAGTACGGA TGTCTTGGGA
    AGTGATTTTG ACCCTAACAT TGACGAAGAT CGCTTGCAAG AAATCGCAGG TGCAGCAGCA
    GAGAACATGT TAGGCAGTTT ACTGTGCCTG CCAGGTTCGG GGTCAGTGCT TCTTGACCCC
    TGCACTGGTT CCACCATATC AGAGACAACA AGCGAAGCTT GGAGTGTAGA GGTATTGCCA
    AGTGACTCAG AGGCCCCAGA TCTAAAGCAG GAAGAGCGAC TGCAGGAACT GGAGAGCTGT
    TCTGGACTGG GTAGCACATC TGATGATACG GATGTCAGGG AGGTCAGTTC CCGCCCCAGC
    ACACCAGGCC TCAGTGTTGT GTCGGGCATA AGTGCAACCT CTGAGGATAT TCCCAATAAG
    ATTGAAGATC TGAGATCTGA GTGCAGCTCT GATTTTGGGG GTAAAGATTC TGTCACTAGT
    CCAGACATGG ATGAAGTCAC CCATGGTGCC CACCAGCTGA CCTCTCCTCC GTCTCAGTCA
    GAGTCTCTGC TTGCCATGTT TGATCCATTG TCTTCGCACG AAGGGGGAGC TTCTGCTGTG
    GTAAGGCCGA AGGTTCACTA TGCCCGGCCG TCGCACCCAC CCCCAGACCC CCCGATCCTG
    GAAGGAGCTG TGGGAGGAAA CGAGGCCAGG TTGCCTACCT TCGGTTCCCA TATCCTGACT
    CCAGCTGAGA TGGAGGCGTT TAAGCAAAGG CACTCGTACC CCGAGAGATT AGTTCGCAGC
    AGGAGCTCCG ACGTCGTGTC TTCTGTCCGG CGCCCGATGA GCGACCCCAG CTGGAACCGA
    CGCCCAGGGA ATGAAGAGCG AGAACTCCCT CCAGCTGCAG CCATTGGTGC TACTTCTTTG
    GTGGCTGCGC CTCACTCTTC ATCTTCATCC CCGAGTAAGG ACTCCTCAAG AGGAGAGACT
    GAAGAACGCA AAGATAGCGA TGATGAGAAA TCAGACAGGA ACAGACCTTG GTGGAGAAAA
    CGTTTTGTTT CTGCCATGCC CAAAGCTCCT ATACCATTTA GAAAGAAAGA AAAACAAGAA
    AAAGACAAAG ATGATCTGGG GCCTGACAGA TTCTCAACAC TCACAGATGA TCCCAGCCCG
    AGACTCAGTG CACAGGCTCA GGTCGCTGAG GATATTCTGG ACAAATACAG GAATGCCATC
    AAACGCACCA GCCCCAGTGA GGGAGCTCTG GCAAACTACG AAAGTGCAGA AGTCATGGGT
    GACGGTGAAA GCGCACACGA CTCTCCTCGT GACGAAACCC TGCAGAACAT CTCCGCTGAC
    GATCTCCCAG ATTCTGCGAG CCAAGCGGCT CACCCTCAGG ATTCAGCCTT CTCTTACAGA
    GATGCAAAAA AGAAACTGAG GCTCGCTCTT TGCTCTGCGG ATTCTGTGGC TTTCCCAGTG
    CTAACCCACT CAACAAGAAA CGGTTTACCA GACCATACAG ACCCAGAAGA CAATGAAATT
    GTCTGCTTCT TAAAAGTGCA AATAGCTGAA GCAATTAACT TACAAGATAA GAACTTAATG
    GCTCAGCTTC AGGAAACAAT GCGTTGTGTG TGCCGCTTTG ATAACCGGAC CTGTAGGAAG
    CTGCTGGCGT CCATCGCCGA GGACTACAGC GTCTTTCCTT TCAGGAAGCG GGCCCCCTAC
    ATTGCTTACC TCACTCGCTG TCGACAAGGA CTGCAGACCA CGCAGGCTCA CCTGGAAAGG
    CTACTGCAGA GGGTTCTGCG GGACAAAGAG GTGGCCAATC GATACTTCAC CACCGTCTGT
    GTGAGGCTGC TGCTTGAGAG CAAAGAGAAG AAGATCAGGG AATTCATTCA AGACTTTCAG
    AAACTCACAG CCGCTGATGA TAAAACCGCC CAAGTGGAAG ATTTTCTGCA GTTCCTGTAC
    GGCGCGATGG CCCAGGACGT CATATGGCAG AACGCGAGTG AGGAACAGCT TCAGGACGCT
    CAGCTGGCCA TTGAGAGAAG TGTGATGAAC CGGATCTTCA AACTTGCCTT CTACCCAAAT
    CAGGACGGGG ACATACTCCG TGACCAGGTT CTTCATGAAC ATATTCAGAG ATTGTCTAAA
    GTAGTGACTG CAAATCACAG AGCTCTGCAG ATACCAGAGG TTTATCTTAA GGAGGCACCG
    TGGCCCTCGG CACAGTCAGA AATCAGGACA ATAAGTGCTT ACAAGACGCC CCGGGACAAA
    GTGCAGTGCA TCCTGAGAAT GTGCTCCACG ATCATGAACC TCCTGAGCCT GGCCAACGAG
    GACTCCGTCC CAGGGGCAGA TGACTTCGTC CCTGTGCTGG TGTTTGTGTT GATCAAGGCA
    AATCCACCCT GTTTGCTGTC CACTGTTCAG TACATCAGTA GCTTTTATGC CAGCTGTCTG
    TCTGGAGAGG AGTCCTATTG GTGGATGCAG TTCACGGCGG CCGTGGAATT CATTAAGACT
    ATCGATGACC GAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808655.1
    CDS174..4511
    Translation

    Target ORF information:

    RefSeq Version XM_010810353.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810353.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TCCTTCAAAT
    CGTTCTAATT CAGTGTCCTC ACTAGACCTG GAGGGGGAAT CTGTGTCAGA GCTTGGAGCA
    GGACCTTCTG GGAGTAATGG AGTTGAAGCT CTACAGCTGT TAGAGCATGA GCAAGCTACC
    ACGCAGGATA ATCTTGATGA TAAGCTCCGG AAGTTTGAAA TCCGTGACAT GATGGGACTG
    ACGGATGACA GGGATATATC AGAAACAGTG AGTGAGACGT GGAGTACGGA TGTCTTGGGA
    AGTGATTTTG ACCCTAACAT TGACGAAGAT CGCTTGCAAG AAATCGCAGG TGCAGCAGCA
    GAGAACATGT TAGGCAGTTT ACTGTGCCTG CCAGGTTCGG GGTCAGTGCT TCTTGACCCC
    TGCACTGGTT CCACCATATC AGAGACAACA AGCGAAGCTT GGAGTGTAGA GGTATTGCCA
    AGTGACTCAG AGGCCCCAGA TCTAAAGCAG GAAGAGCGAC TGCAGGAACT GGAGAGCTGT
    TCTGGACTGG GTAGCACATC TGATGATACG GATGTCAGGG AGGTCAGTTC CCGCCCCAGC
    ACACCAGGCC TCAGTGTTGT GTCGGGCATA AGTGCAACCT CTGAGGATAT TCCCAATAAG
    ATTGAAGATC TGAGATCTGA GTGCAGCTCT GATTTTGGGG GTAAAGATTC TGTCACTAGT
    CCAGACATGG ATGAAGTCAC CCATGGTGCC CACCAGCTGA CCTCTCCTCC GTCTCAGTCA
    GAGTCTCTGC TTGCCATGTT TGATCCATTG TCTTCGCACG AAGGGGGAGC TTCTGCTGTG
    GTAAGGCCGA AGGTTCACTA TGCCCGGCCG TCGCACCCAC CCCCAGACCC CCCGATCCTG
    GAAGGAGCTG TGGGAGGAAA CGAGGCCAGG TTGCCTACCT TCGGTTCCCA TATCCTGACT
    CCAGCTGAGA TGGAGGCGTT TAAGCAAAGG CACTCGTACC CCGAGAGATT AGTTCGCAGC
    AGGAGCTCCG ACGTCGTGTC TTCTGTCCGG CGCCCGATGA GCGACCCCAG CTGGAACCGA
    CGCCCAGGGA ATGAAGAGCG AGAACTCCCT CCAGCTGCAG CCATTGGTGC TACTTCTTTG
    GTGGCTGCGC CTCACTCTTC ATCTTCATCC CCGAGTAAGG ACTCCTCAAG AGGAGAGACT
    GAAGAACGCA AAGATAGCGA TGATGAGAAA TCAGACAGGA ACAGACCTTG GTGGAGAAAA
    CGTTTTGTTT CTGCCATGCC CAAAGCTCCT ATACCATTTA GAAAGAAAGA AAAACAAGAA
    AAAGACAAAG ATGATCTGGG GCCTGACAGA TTCTCAACAC TCACAGATGA TCCCAGCCCG
    AGACTCAGTG CACAGGCTCA GGTCGCTGAG GATATTCTGG ACAAATACAG GAATGCCATC
    AAACGCACCA GCCCCAGTGA GGGAGCTCTG GCAAACTACG AAAGTGCAGA AGTCATGGGT
    GACGGTGAAA GCGCACACGA CTCTCCTCGT GACGAAACCC TGCAGAACAT CTCCGCTGAC
    GATCTCCCAG ATTCTGCGAG CCAAGCGGCT CACCCTCAGG ATTCAGCCTT CTCTTACAGA
    GATGCAAAAA AGAAACTGAG GCTCGCTCTT TGCTCTGCGG ATTCTGTGGC TTTCCCAGTG
    CTAACCCACT CAACAAGAAA CGGTTTACCA GACCATACAG ACCCAGAAGA CAATGAAATT
    GTCTGCTTCT TAAAAGTGCA AATAGCTGAA GCAATTAACT TACAAGATAA GAACTTAATG
    GCTCAGCTTC AGGAAACAAT GCGTTGTGTG TGCCGCTTTG ATAACCGGAC CTGTAGGAAG
    CTGCTGGCGT CCATCGCCGA GGACTACAGC GTCTTTCCTT TCAGGAAGCG GGCCCCCTAC
    ATTGCTTACC TCACTCGCTG TCGACAAGGA CTGCAGACCA CGCAGGCTCA CCTGGAAAGG
    CTACTGCAGA GGGTTCTGCG GGACAAAGAG GTGGCCAATC GATACTTCAC CACCGTCTGT
    GTGAGGCTGC TGCTTGAGAG CAAAGAGAAG AAGATCAGGG AATTCATTCA AGACTTTCAG
    AAACTCACAG CCGCTGATGA TAAAACCGCC CAAGTGGAAG ATTTTCTGCA GTTCCTGTAC
    GGCGCGATGG CCCAGGACGT CATATGGCAG AACGCGAGTG AGGAACAGCT TCAGGACGCT
    CAGCTGGCCA TTGAGAGAAG TGTGATGAAC CGGATCTTCA AACTTGCCTT CTACCCAAAT
    CAGGACGGGG ACATACTCCG TGACCAGGTT CTTCATGAAC ATATTCAGAG ATTGTCTAAA
    GTAGTGACTG CAAATCACAG AGCTCTGCAG ATACCAGAGG TTTATCTTAA GGAGGCACCG
    TGGCCCTCGG CACAGTCAGA AATCAGGACA ATAAGTGCTT ACAAGACGCC CCGGGACAAA
    GTGCAGTGCA TCCTGAGAAT GTGCTCCACG ATCATGAACC TCCTGAGCCT GGCCAACGAG
    GACTCCGTCC CAGGGGCAGA TGACTTCGTC CCTGTGCTGG TGTTTGTGTT GATCAAGGCA
    AATCCACCCT GTTTGCTGTC CACTGTTCAG TACATCAGTA GCTTTTATGC CAGCTGTCTG
    TCTGGAGAGG AGTCCTATTG GTGGATGCAG TTCACGGCGG CCGTGGAATT CATTAAGACT
    ATCGATGACC GAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54404
    Clone ID Related Accession (Same CDS sequence) XM_010810353.2 , XM_010810353.3
    Accession Version XM_010810353.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810353.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TCCTTCAAAT
    CGTTCTAATT CAGTGTCCTC ACTAGACCTG GAGGGGGAAT CTGTGTCAGA GCTTGGAGCA
    GGACCTTCTG GGAGTAATGG AGTTGAAGCT CTACAGCTGT TAGAGCATGA GCAAGCTACC
    ACGCAGGATA ATCTTGATGA TAAGCTCCGG AAGTTTGAAA TCCGTGACAT GATGGGACTG
    ACGGATGACA GGGATATATC AGAAACAGTG AGTGAGACGT GGAGTACGGA TGTCTTGGGA
    AGTGATTTTG ACCCTAACAT TGACGAAGAT CGCTTGCAAG AAATCGCAGG TGCAGCAGCA
    GAGAACATGT TAGGCAGTTT ACTGTGCCTG CCAGGTTCGG GGTCAGTGCT TCTTGACCCC
    TGCACTGGTT CCACCATATC AGAGACAACA AGCGAAGCTT GGAGTGTAGA GGTATTGCCA
    AGTGACTCAG AGGCCCCAGA TCTAAAGCAG GAAGAGCGAC TGCAGGAACT GGAGAGCTGT
    TCTGGACTGG GTAGCACATC TGATGATACG GATGTCAGGG AGGTCAGTTC CCGCCCCAGC
    ACACCAGGCC TCAGTGTTGT GTCGGGCATA AGTGCAACCT CTGAGGATAT TCCCAATAAG
    ATTGAAGATC TGAGATCTGA GTGCAGCTCT GATTTTGGGG GTAAAGATTC TGTCACTAGT
    CCAGACATGG ATGAAGTCAC CCATGGTGCC CACCAGCTGA CCTCTCCTCC GTCTCAGTCA
    GAGTCTCTGC TTGCCATGTT TGATCCATTG TCTTCGCACG AAGGGGGAGC TTCTGCTGTG
    GTAAGGCCGA AGGTTCACTA TGCCCGGCCG TCGCACCCAC CCCCAGACCC CCCGATCCTG
    GAAGGAGCTG TGGGAGGAAA CGAGGCCAGG TTGCCTACCT TCGGTTCCCA TATCCTGACT
    CCAGCTGAGA TGGAGGCGTT TAAGCAAAGG CACTCGTACC CCGAGAGATT AGTTCGCAGC
    AGGAGCTCCG ACGTCGTGTC TTCTGTCCGG CGCCCGATGA GCGACCCCAG CTGGAACCGA
    CGCCCAGGGA ATGAAGAGCG AGAACTCCCT CCAGCTGCAG CCATTGGTGC TACTTCTTTG
    GTGGCTGCGC CTCACTCTTC ATCTTCATCC CCGAGTAAGG ACTCCTCAAG AGGAGAGACT
    GAAGAACGCA AAGATAGCGA TGATGAGAAA TCAGACAGGA ACAGACCTTG GTGGAGAAAA
    CGTTTTGTTT CTGCCATGCC CAAAGCTCCT ATACCATTTA GAAAGAAAGA AAAACAAGAA
    AAAGACAAAG ATGATCTGGG GCCTGACAGA TTCTCAACAC TCACAGATGA TCCCAGCCCG
    AGACTCAGTG CACAGGCTCA GGTCGCTGAG GATATTCTGG ACAAATACAG GAATGCCATC
    AAACGCACCA GCCCCAGTGA GGGAGCTCTG GCAAACTACG AAAGTGCAGA AGTCATGGGT
    GACGGTGAAA GCGCACACGA CTCTCCTCGT GACGAAACCC TGCAGAACAT CTCCGCTGAC
    GATCTCCCAG ATTCTGCGAG CCAAGCGGCT CACCCTCAGG ATTCAGCCTT CTCTTACAGA
    GATGCAAAAA AGAAACTGAG GCTCGCTCTT TGCTCTGCGG ATTCTGTGGC TTTCCCAGTG
    CTAACCCACT CAACAAGAAA CGGTTTACCA GACCATACAG ACCCAGAAGA CAATGAAATT
    GTCTGCTTCT TAAAAGTGCA AATAGCTGAA GCAATTAACT TACAAGATAA GAACTTAATG
    GCTCAGCTTC AGGAAACAAT GCGTTGTGTG TGCCGCTTTG ATAACCGGAC CTGTAGGAAG
    CTGCTGGCGT CCATCGCCGA GGACTACAGC GTCTTTCCTT TCAGGAAGCG GGCCCCCTAC
    ATTGCTTACC TCACTCGCTG TCGACAAGGA CTGCAGACCA CGCAGGCTCA CCTGGAAAGG
    CTACTGCAGA GGGTTCTGCG GGACAAAGAG GTGGCCAATC GATACTTCAC CACCGTCTGT
    GTGAGGCTGC TGCTTGAGAG CAAAGAGAAG AAGATCAGGG AATTCATTCA AGACTTTCAG
    AAACTCACAG CCGCTGATGA TAAAACCGCC CAAGTGGAAG ATTTTCTGCA GTTCCTGTAC
    GGCGCGATGG CCCAGGACGT CATATGGCAG AACGCGAGTG AGGAACAGCT TCAGGACGCT
    CAGCTGGCCA TTGAGAGAAG TGTGATGAAC CGGATCTTCA AACTTGCCTT CTACCCAAAT
    CAGGACGGGG ACATACTCCG TGACCAGGTT CTTCATGAAC ATATTCAGAG ATTGTCTAAA
    GTAGTGACTG CAAATCACAG AGCTCTGCAG ATACCAGAGG TTTATCTTAA GGAGGCACCG
    TGGCCCTCGG CACAGTCAGA AATCAGGACA ATAAGTGCTT ACAAGACGCC CCGGGACAAA
    GTGCAGTGCA TCCTGAGAAT GTGCTCCACG ATCATGAACC TCCTGAGCCT GGCCAACGAG
    GACTCCGTCC CAGGGGCAGA TGACTTCGTC CCTGTGCTGG TGTTTGTGTT GATCAAGGCA
    AATCCACCCT GTTTGCTGTC CACTGTTCAG TACATCAGTA GCTTTTATGC CAGCTGTCTG
    TCTGGAGAGG AGTCCTATTG GTGGATGCAG TTCACGGCGG CCGTGGAATT CATTAAGACT
    ATCGATGACC GAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808655.1
    CDS170..4507
    Translation

    Target ORF information:

    RefSeq Version XM_010810353.3
    Organism Bos taurus(cattle)
    Definition Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810353.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TCCTTCAAAT
    CGTTCTAATT CAGTGTCCTC ACTAGACCTG GAGGGGGAAT CTGTGTCAGA GCTTGGAGCA
    GGACCTTCTG GGAGTAATGG AGTTGAAGCT CTACAGCTGT TAGAGCATGA GCAAGCTACC
    ACGCAGGATA ATCTTGATGA TAAGCTCCGG AAGTTTGAAA TCCGTGACAT GATGGGACTG
    ACGGATGACA GGGATATATC AGAAACAGTG AGTGAGACGT GGAGTACGGA TGTCTTGGGA
    AGTGATTTTG ACCCTAACAT TGACGAAGAT CGCTTGCAAG AAATCGCAGG TGCAGCAGCA
    GAGAACATGT TAGGCAGTTT ACTGTGCCTG CCAGGTTCGG GGTCAGTGCT TCTTGACCCC
    TGCACTGGTT CCACCATATC AGAGACAACA AGCGAAGCTT GGAGTGTAGA GGTATTGCCA
    AGTGACTCAG AGGCCCCAGA TCTAAAGCAG GAAGAGCGAC TGCAGGAACT GGAGAGCTGT
    TCTGGACTGG GTAGCACATC TGATGATACG GATGTCAGGG AGGTCAGTTC CCGCCCCAGC
    ACACCAGGCC TCAGTGTTGT GTCGGGCATA AGTGCAACCT CTGAGGATAT TCCCAATAAG
    ATTGAAGATC TGAGATCTGA GTGCAGCTCT GATTTTGGGG GTAAAGATTC TGTCACTAGT
    CCAGACATGG ATGAAGTCAC CCATGGTGCC CACCAGCTGA CCTCTCCTCC GTCTCAGTCA
    GAGTCTCTGC TTGCCATGTT TGATCCATTG TCTTCGCACG AAGGGGGAGC TTCTGCTGTG
    GTAAGGCCGA AGGTTCACTA TGCCCGGCCG TCGCACCCAC CCCCAGACCC CCCGATCCTG
    GAAGGAGCTG TGGGAGGAAA CGAGGCCAGG TTGCCTACCT TCGGTTCCCA TATCCTGACT
    CCAGCTGAGA TGGAGGCGTT TAAGCAAAGG CACTCGTACC CCGAGAGATT AGTTCGCAGC
    AGGAGCTCCG ACGTCGTGTC TTCTGTCCGG CGCCCGATGA GCGACCCCAG CTGGAACCGA
    CGCCCAGGGA ATGAAGAGCG AGAACTCCCT CCAGCTGCAG CCATTGGTGC TACTTCTTTG
    GTGGCTGCGC CTCACTCTTC ATCTTCATCC CCGAGTAAGG ACTCCTCAAG AGGAGAGACT
    GAAGAACGCA AAGATAGCGA TGATGAGAAA TCAGACAGGA ACAGACCTTG GTGGAGAAAA
    CGTTTTGTTT CTGCCATGCC CAAAGCTCCT ATACCATTTA GAAAGAAAGA AAAACAAGAA
    AAAGACAAAG ATGATCTGGG GCCTGACAGA TTCTCAACAC TCACAGATGA TCCCAGCCCG
    AGACTCAGTG CACAGGCTCA GGTCGCTGAG GATATTCTGG ACAAATACAG GAATGCCATC
    AAACGCACCA GCCCCAGTGA GGGAGCTCTG GCAAACTACG AAAGTGCAGA AGTCATGGGT
    GACGGTGAAA GCGCACACGA CTCTCCTCGT GACGAAACCC TGCAGAACAT CTCCGCTGAC
    GATCTCCCAG ATTCTGCGAG CCAAGCGGCT CACCCTCAGG ATTCAGCCTT CTCTTACAGA
    GATGCAAAAA AGAAACTGAG GCTCGCTCTT TGCTCTGCGG ATTCTGTGGC TTTCCCAGTG
    CTAACCCACT CAACAAGAAA CGGTTTACCA GACCATACAG ACCCAGAAGA CAATGAAATT
    GTCTGCTTCT TAAAAGTGCA AATAGCTGAA GCAATTAACT TACAAGATAA GAACTTAATG
    GCTCAGCTTC AGGAAACAAT GCGTTGTGTG TGCCGCTTTG ATAACCGGAC CTGTAGGAAG
    CTGCTGGCGT CCATCGCCGA GGACTACAGC GTCTTTCCTT TCAGGAAGCG GGCCCCCTAC
    ATTGCTTACC TCACTCGCTG TCGACAAGGA CTGCAGACCA CGCAGGCTCA CCTGGAAAGG
    CTACTGCAGA GGGTTCTGCG GGACAAAGAG GTGGCCAATC GATACTTCAC CACCGTCTGT
    GTGAGGCTGC TGCTTGAGAG CAAAGAGAAG AAGATCAGGG AATTCATTCA AGACTTTCAG
    AAACTCACAG CCGCTGATGA TAAAACCGCC CAAGTGGAAG ATTTTCTGCA GTTCCTGTAC
    GGCGCGATGG CCCAGGACGT CATATGGCAG AACGCGAGTG AGGAACAGCT TCAGGACGCT
    CAGCTGGCCA TTGAGAGAAG TGTGATGAAC CGGATCTTCA AACTTGCCTT CTACCCAAAT
    CAGGACGGGG ACATACTCCG TGACCAGGTT CTTCATGAAC ATATTCAGAG ATTGTCTAAA
    GTAGTGACTG CAAATCACAG AGCTCTGCAG ATACCAGAGG TTTATCTTAA GGAGGCACCG
    TGGCCCTCGG CACAGTCAGA AATCAGGACA ATAAGTGCTT ACAAGACGCC CCGGGACAAA
    GTGCAGTGCA TCCTGAGAAT GTGCTCCACG ATCATGAACC TCCTGAGCCT GGCCAACGAG
    GACTCCGTCC CAGGGGCAGA TGACTTCGTC CCTGTGCTGG TGTTTGTGTT GATCAAGGCA
    AATCCACCCT GTTTGCTGTC CACTGTTCAG TACATCAGTA GCTTTTATGC CAGCTGTCTG
    TCTGGAGAGG AGTCCTATTG GTGGATGCAG TTCACGGCGG CCGTGGAATT CATTAAGACT
    ATCGATGACC GAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54405
    Clone ID Related Accession (Same CDS sequence) XM_015473586.1 , XM_024999023.1
    Accession Version XM_015473586.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104301.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TCCTTCAAAT
    CGTTCTAATT CAGTGTCCTC ACTAGACCTG GAGGGGGAAT CTGTGTCAGA GCTTGGAGCA
    GGACCTTCTG GGAGTAATGG AGTTGAAGCT CTACAGCTGT TAGAGCATGA GCAAGCTACC
    ACGCAGGATA ATCTTGATGA TAAGCTCCGG AAGTTTGAAA TCCGTGACAT GATGGGACTG
    ACGGATGACA GGGATATATC AGAAACAGTG AGTGAGACGT GGAGTACGGA TGTCTTGGGA
    AGTGATTTTG ACCCTAACAT TGACGAAGAT CGCTTGCAAG AAATCGCAGG TGCAGCAGCA
    GAGAACATGT TAGGCAGTTT ACTGTGCCTG CCAGGTTCGG GGTCAGTGCT TCTTGACCCC
    TGCACTGGTT CCACCATATC AGAGACAACA AGCGAAGCTT GGAGTGTAGA GGTATTGCCA
    AGTGACTCAG AGGCCCCAGA TCTAAAGCAG GAAGAGCGAC TGCAGGAACT GGAGAGCTGT
    TCTGGACTGG GTAGCACATC TGATGATACG GATGTCAGGG AGGTCAGTTC CCGCCCCAGC
    ACACCAGGCC TCAGTGTTGT GTCGGGCATA AGTGCAACCT CTGAGGATAT TCCCAATAAG
    ATTGAAGATC TGAGATCTGA GTGCAGCTCT GATTTTGGGG GTAAAGATTC TGTCACTAGT
    CCAGACATGG ATGAAGTCAC CCATGGTGCC CACCAGCTGA CCTCTCCTCC GTCTCAGTCA
    GAGTCTCTGC TTGCCATGTT TGATCCATTG TCTTCGCACG AAGGGGGAGC TTCTGCTGTG
    GTAAGGCCGA AGGTTCACTA TGCCCGGCCG TCGCACCCAC CCCCAGACCC CCCGATCCTG
    GAAGGAGCTG TGGGAGGAAA CGAGGCCAGG TTGCCTACCT TCGGTTCCCA TATCCTGACT
    CCAGCTGAGA TGGAGGCGTT TAAGCAAAGG CACTCGTACC CCGAGAGATT AGTTCGCAGC
    AGGAGCTCCG ACGTCGTGTC TTCTGTCCGG CGCCCGATGA GCGACCCCAG CTGGAACCGA
    CGCCCAGGGA ATGAAGAGCG AGAACTCCCT CCAGCTGCAG CCATTGGTGC TACTTCTTTG
    GTGGCTGCGC CTCACTCTTC ATCTTCATCC CCGAGTAAGG ACTCCTCAAG AGGAGAGACT
    GAAGAACGCA AAGATAGCGA TGATGAGAAA TCAGACAGGA ACAGACCTTG GTGGAGAAAA
    CGTTTTGTTT CTGCCATGCC CAAAGCTCCT ATACCATTTA GAAAGAAAGA AAAACAAGAA
    AAAGACAAAG ATGATCTGGG GCCTGACAGA TTCTCAACAC TCACAGATGA TCCCAGCCCG
    AGACTCAGTG CACAGGCTCA GGTCGCTGAG GATATTCTGG ACAAATACAG GAATGCCATC
    AAACGCACCA GCCCCAGTGA GGGAGCTCTG GCAAACTACG AAAGTGCAGA AGTCATGGGT
    GACGGTGAAA GCGCACACGA CTCTCCTCGT GACGAAACCC TGCAGAACAT CTCCGCTGAC
    GATCTCCCAG ATTCTGCGAG CCAAGCGGCT CACCCTCAGG ATTCAGCCTT CTCTTACAGA
    GATGCAAAAA AGAAACTGAG GCTCGCTCTT TGCTCTGCGG ATTCTGTGGC TTTCCCAGTG
    CTAACCCACT CAACAAGAAA CGGTTTACCA GACCATACAG ACCCAGAAGA CAATGAAATT
    GTCTGCTTCT TAAAAGTGCA AATAGCTGAA GCAATTAACT TACAAGATAA GAACTTAATG
    GCTCAGCTTC AGGAAACAAT GCGTTGTGTG TGCCGCTTTG ATAACCGGAC CTGTAGGAAG
    CTGCTGGCGT CCATCGCCGA GGACTACAGG AAGCGGGCCC CCTACATTGC TTACCTCACT
    CGCTGTCGAC AAGGACTGCA GACCACGCAG GCTCACCTGG AAAGGCTACT GCAGAGGGTT
    CTGCGGGACA AAGAGGTGGC CAATCGATAC TTCACCACCG TCTGTGTGAG GCTGCTGCTT
    GAGAGCAAAG AGAAGAAGAT CAGGGAATTC ATTCAAGACT TTCAGAAACT CACAGCCGCT
    GATGATAAAA CCGCCCAAGT GGAAGATTTT CTGCAGTTCC TGTACGGCGC GATGGCCCAG
    GACGTCATAT GGCAGAACGC GAGTGAGGAA CAGCTTCAGG ACGCTCAGCT GGCCATTGAG
    AGAAGTGTGA TGAACCGGAT CTTCAAACTT GCCTTCTACC CAAATCAGGA CGGGGACATA
    CTCCGTGACC AGGTTCTTCA TGAACATATT CAGAGATTGT CTAAAGTAGT GACTGCAAAT
    CACAGAGCTC TGCAGATACC AGAGGTTTAT CTTAAGGAGG CACCGTGGCC CTCGGCACAG
    TCAGAAATCA GGACAATAAG TGCTTACAAG ACGCCCCGGG ACAAAGTGCA GTGCATCCTG
    AGAATGTGCT CCACGATCAT GAACCTCCTG AGCCTGGCCA ACGAGGACTC CGTCCCAGGG
    GCAGATGACT TCGTCCCTGT GCTGGTGTTT GTGTTGATCA AGGCAAATCC ACCCTGTTTG
    CTGTCCACTG TTCAGTACAT CAGTAGCTTT TATGCCAGCT GTCTGTCTGG AGAGGAGTCC
    TATTGGTGGA TGCAGTTCAC GGCGGCCGTG GAATTCATTA AGACTATCGA TGACCGAAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329072.1
    CDS1..4323
    Translation

    Target ORF information:

    RefSeq Version XM_015473586.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473586.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TCCTTCAAAT
    CGTTCTAATT CAGTGTCCTC ACTAGACCTG GAGGGGGAAT CTGTGTCAGA GCTTGGAGCA
    GGACCTTCTG GGAGTAATGG AGTTGAAGCT CTACAGCTGT TAGAGCATGA GCAAGCTACC
    ACGCAGGATA ATCTTGATGA TAAGCTCCGG AAGTTTGAAA TCCGTGACAT GATGGGACTG
    ACGGATGACA GGGATATATC AGAAACAGTG AGTGAGACGT GGAGTACGGA TGTCTTGGGA
    AGTGATTTTG ACCCTAACAT TGACGAAGAT CGCTTGCAAG AAATCGCAGG TGCAGCAGCA
    GAGAACATGT TAGGCAGTTT ACTGTGCCTG CCAGGTTCGG GGTCAGTGCT TCTTGACCCC
    TGCACTGGTT CCACCATATC AGAGACAACA AGCGAAGCTT GGAGTGTAGA GGTATTGCCA
    AGTGACTCAG AGGCCCCAGA TCTAAAGCAG GAAGAGCGAC TGCAGGAACT GGAGAGCTGT
    TCTGGACTGG GTAGCACATC TGATGATACG GATGTCAGGG AGGTCAGTTC CCGCCCCAGC
    ACACCAGGCC TCAGTGTTGT GTCGGGCATA AGTGCAACCT CTGAGGATAT TCCCAATAAG
    ATTGAAGATC TGAGATCTGA GTGCAGCTCT GATTTTGGGG GTAAAGATTC TGTCACTAGT
    CCAGACATGG ATGAAGTCAC CCATGGTGCC CACCAGCTGA CCTCTCCTCC GTCTCAGTCA
    GAGTCTCTGC TTGCCATGTT TGATCCATTG TCTTCGCACG AAGGGGGAGC TTCTGCTGTG
    GTAAGGCCGA AGGTTCACTA TGCCCGGCCG TCGCACCCAC CCCCAGACCC CCCGATCCTG
    GAAGGAGCTG TGGGAGGAAA CGAGGCCAGG TTGCCTACCT TCGGTTCCCA TATCCTGACT
    CCAGCTGAGA TGGAGGCGTT TAAGCAAAGG CACTCGTACC CCGAGAGATT AGTTCGCAGC
    AGGAGCTCCG ACGTCGTGTC TTCTGTCCGG CGCCCGATGA GCGACCCCAG CTGGAACCGA
    CGCCCAGGGA ATGAAGAGCG AGAACTCCCT CCAGCTGCAG CCATTGGTGC TACTTCTTTG
    GTGGCTGCGC CTCACTCTTC ATCTTCATCC CCGAGTAAGG ACTCCTCAAG AGGAGAGACT
    GAAGAACGCA AAGATAGCGA TGATGAGAAA TCAGACAGGA ACAGACCTTG GTGGAGAAAA
    CGTTTTGTTT CTGCCATGCC CAAAGCTCCT ATACCATTTA GAAAGAAAGA AAAACAAGAA
    AAAGACAAAG ATGATCTGGG GCCTGACAGA TTCTCAACAC TCACAGATGA TCCCAGCCCG
    AGACTCAGTG CACAGGCTCA GGTCGCTGAG GATATTCTGG ACAAATACAG GAATGCCATC
    AAACGCACCA GCCCCAGTGA GGGAGCTCTG GCAAACTACG AAAGTGCAGA AGTCATGGGT
    GACGGTGAAA GCGCACACGA CTCTCCTCGT GACGAAACCC TGCAGAACAT CTCCGCTGAC
    GATCTCCCAG ATTCTGCGAG CCAAGCGGCT CACCCTCAGG ATTCAGCCTT CTCTTACAGA
    GATGCAAAAA AGAAACTGAG GCTCGCTCTT TGCTCTGCGG ATTCTGTGGC TTTCCCAGTG
    CTAACCCACT CAACAAGAAA CGGTTTACCA GACCATACAG ACCCAGAAGA CAATGAAATT
    GTCTGCTTCT TAAAAGTGCA AATAGCTGAA GCAATTAACT TACAAGATAA GAACTTAATG
    GCTCAGCTTC AGGAAACAAT GCGTTGTGTG TGCCGCTTTG ATAACCGGAC CTGTAGGAAG
    CTGCTGGCGT CCATCGCCGA GGACTACAGG AAGCGGGCCC CCTACATTGC TTACCTCACT
    CGCTGTCGAC AAGGACTGCA GACCACGCAG GCTCACCTGG AAAGGCTACT GCAGAGGGTT
    CTGCGGGACA AAGAGGTGGC CAATCGATAC TTCACCACCG TCTGTGTGAG GCTGCTGCTT
    GAGAGCAAAG AGAAGAAGAT CAGGGAATTC ATTCAAGACT TTCAGAAACT CACAGCCGCT
    GATGATAAAA CCGCCCAAGT GGAAGATTTT CTGCAGTTCC TGTACGGCGC GATGGCCCAG
    GACGTCATAT GGCAGAACGC GAGTGAGGAA CAGCTTCAGG ACGCTCAGCT GGCCATTGAG
    AGAAGTGTGA TGAACCGGAT CTTCAAACTT GCCTTCTACC CAAATCAGGA CGGGGACATA
    CTCCGTGACC AGGTTCTTCA TGAACATATT CAGAGATTGT CTAAAGTAGT GACTGCAAAT
    CACAGAGCTC TGCAGATACC AGAGGTTTAT CTTAAGGAGG CACCGTGGCC CTCGGCACAG
    TCAGAAATCA GGACAATAAG TGCTTACAAG ACGCCCCGGG ACAAAGTGCA GTGCATCCTG
    AGAATGTGCT CCACGATCAT GAACCTCCTG AGCCTGGCCA ACGAGGACTC CGTCCCAGGG
    GCAGATGACT TCGTCCCTGT GCTGGTGTTT GTGTTGATCA AGGCAAATCC ACCCTGTTTG
    CTGTCCACTG TTCAGTACAT CAGTAGCTTT TATGCCAGCT GTCTGTCTGG AGAGGAGTCC
    TATTGGTGGA TGCAGTTCAC GGCGGCCGTG GAATTCATTA AGACTATCGA TGACCGAAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54405
    Clone ID Related Accession (Same CDS sequence) XM_015473586.1 , XM_024999023.1
    Accession Version XM_024999023.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TCCTTCAAAT
    CGTTCTAATT CAGTGTCCTC ACTAGACCTG GAGGGGGAAT CTGTGTCAGA GCTTGGAGCA
    GGACCTTCTG GGAGTAATGG AGTTGAAGCT CTACAGCTGT TAGAGCATGA GCAAGCTACC
    ACGCAGGATA ATCTTGATGA TAAGCTCCGG AAGTTTGAAA TCCGTGACAT GATGGGACTG
    ACGGATGACA GGGATATATC AGAAACAGTG AGTGAGACGT GGAGTACGGA TGTCTTGGGA
    AGTGATTTTG ACCCTAACAT TGACGAAGAT CGCTTGCAAG AAATCGCAGG TGCAGCAGCA
    GAGAACATGT TAGGCAGTTT ACTGTGCCTG CCAGGTTCGG GGTCAGTGCT TCTTGACCCC
    TGCACTGGTT CCACCATATC AGAGACAACA AGCGAAGCTT GGAGTGTAGA GGTATTGCCA
    AGTGACTCAG AGGCCCCAGA TCTAAAGCAG GAAGAGCGAC TGCAGGAACT GGAGAGCTGT
    TCTGGACTGG GTAGCACATC TGATGATACG GATGTCAGGG AGGTCAGTTC CCGCCCCAGC
    ACACCAGGCC TCAGTGTTGT GTCGGGCATA AGTGCAACCT CTGAGGATAT TCCCAATAAG
    ATTGAAGATC TGAGATCTGA GTGCAGCTCT GATTTTGGGG GTAAAGATTC TGTCACTAGT
    CCAGACATGG ATGAAGTCAC CCATGGTGCC CACCAGCTGA CCTCTCCTCC GTCTCAGTCA
    GAGTCTCTGC TTGCCATGTT TGATCCATTG TCTTCGCACG AAGGGGGAGC TTCTGCTGTG
    GTAAGGCCGA AGGTTCACTA TGCCCGGCCG TCGCACCCAC CCCCAGACCC CCCGATCCTG
    GAAGGAGCTG TGGGAGGAAA CGAGGCCAGG TTGCCTACCT TCGGTTCCCA TATCCTGACT
    CCAGCTGAGA TGGAGGCGTT TAAGCAAAGG CACTCGTACC CCGAGAGATT AGTTCGCAGC
    AGGAGCTCCG ACGTCGTGTC TTCTGTCCGG CGCCCGATGA GCGACCCCAG CTGGAACCGA
    CGCCCAGGGA ATGAAGAGCG AGAACTCCCT CCAGCTGCAG CCATTGGTGC TACTTCTTTG
    GTGGCTGCGC CTCACTCTTC ATCTTCATCC CCGAGTAAGG ACTCCTCAAG AGGAGAGACT
    GAAGAACGCA AAGATAGCGA TGATGAGAAA TCAGACAGGA ACAGACCTTG GTGGAGAAAA
    CGTTTTGTTT CTGCCATGCC CAAAGCTCCT ATACCATTTA GAAAGAAAGA AAAACAAGAA
    AAAGACAAAG ATGATCTGGG GCCTGACAGA TTCTCAACAC TCACAGATGA TCCCAGCCCG
    AGACTCAGTG CACAGGCTCA GGTCGCTGAG GATATTCTGG ACAAATACAG GAATGCCATC
    AAACGCACCA GCCCCAGTGA GGGAGCTCTG GCAAACTACG AAAGTGCAGA AGTCATGGGT
    GACGGTGAAA GCGCACACGA CTCTCCTCGT GACGAAACCC TGCAGAACAT CTCCGCTGAC
    GATCTCCCAG ATTCTGCGAG CCAAGCGGCT CACCCTCAGG ATTCAGCCTT CTCTTACAGA
    GATGCAAAAA AGAAACTGAG GCTCGCTCTT TGCTCTGCGG ATTCTGTGGC TTTCCCAGTG
    CTAACCCACT CAACAAGAAA CGGTTTACCA GACCATACAG ACCCAGAAGA CAATGAAATT
    GTCTGCTTCT TAAAAGTGCA AATAGCTGAA GCAATTAACT TACAAGATAA GAACTTAATG
    GCTCAGCTTC AGGAAACAAT GCGTTGTGTG TGCCGCTTTG ATAACCGGAC CTGTAGGAAG
    CTGCTGGCGT CCATCGCCGA GGACTACAGG AAGCGGGCCC CCTACATTGC TTACCTCACT
    CGCTGTCGAC AAGGACTGCA GACCACGCAG GCTCACCTGG AAAGGCTACT GCAGAGGGTT
    CTGCGGGACA AAGAGGTGGC CAATCGATAC TTCACCACCG TCTGTGTGAG GCTGCTGCTT
    GAGAGCAAAG AGAAGAAGAT CAGGGAATTC ATTCAAGACT TTCAGAAACT CACAGCCGCT
    GATGATAAAA CCGCCCAAGT GGAAGATTTT CTGCAGTTCC TGTACGGCGC GATGGCCCAG
    GACGTCATAT GGCAGAACGC GAGTGAGGAA CAGCTTCAGG ACGCTCAGCT GGCCATTGAG
    AGAAGTGTGA TGAACCGGAT CTTCAAACTT GCCTTCTACC CAAATCAGGA CGGGGACATA
    CTCCGTGACC AGGTTCTTCA TGAACATATT CAGAGATTGT CTAAAGTAGT GACTGCAAAT
    CACAGAGCTC TGCAGATACC AGAGGTTTAT CTTAAGGAGG CACCGTGGCC CTCGGCACAG
    TCAGAAATCA GGACAATAAG TGCTTACAAG ACGCCCCGGG ACAAAGTGCA GTGCATCCTG
    AGAATGTGCT CCACGATCAT GAACCTCCTG AGCCTGGCCA ACGAGGACTC CGTCCCAGGG
    GCAGATGACT TCGTCCCTGT GCTGGTGTTT GTGTTGATCA AGGCAAATCC ACCCTGTTTG
    CTGTCCACTG TTCAGTACAT CAGTAGCTTT TATGCCAGCT GTCTGTCTGG AGAGGAGTCC
    TATTGGTGGA TGCAGTTCAC GGCGGCCGTG GAATTCATTA AGACTATCGA TGACCGAAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854791.1
    CDS179..4501
    Translation

    Target ORF information:

    RefSeq Version XM_024999023.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999023.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TCCTTCAAAT
    CGTTCTAATT CAGTGTCCTC ACTAGACCTG GAGGGGGAAT CTGTGTCAGA GCTTGGAGCA
    GGACCTTCTG GGAGTAATGG AGTTGAAGCT CTACAGCTGT TAGAGCATGA GCAAGCTACC
    ACGCAGGATA ATCTTGATGA TAAGCTCCGG AAGTTTGAAA TCCGTGACAT GATGGGACTG
    ACGGATGACA GGGATATATC AGAAACAGTG AGTGAGACGT GGAGTACGGA TGTCTTGGGA
    AGTGATTTTG ACCCTAACAT TGACGAAGAT CGCTTGCAAG AAATCGCAGG TGCAGCAGCA
    GAGAACATGT TAGGCAGTTT ACTGTGCCTG CCAGGTTCGG GGTCAGTGCT TCTTGACCCC
    TGCACTGGTT CCACCATATC AGAGACAACA AGCGAAGCTT GGAGTGTAGA GGTATTGCCA
    AGTGACTCAG AGGCCCCAGA TCTAAAGCAG GAAGAGCGAC TGCAGGAACT GGAGAGCTGT
    TCTGGACTGG GTAGCACATC TGATGATACG GATGTCAGGG AGGTCAGTTC CCGCCCCAGC
    ACACCAGGCC TCAGTGTTGT GTCGGGCATA AGTGCAACCT CTGAGGATAT TCCCAATAAG
    ATTGAAGATC TGAGATCTGA GTGCAGCTCT GATTTTGGGG GTAAAGATTC TGTCACTAGT
    CCAGACATGG ATGAAGTCAC CCATGGTGCC CACCAGCTGA CCTCTCCTCC GTCTCAGTCA
    GAGTCTCTGC TTGCCATGTT TGATCCATTG TCTTCGCACG AAGGGGGAGC TTCTGCTGTG
    GTAAGGCCGA AGGTTCACTA TGCCCGGCCG TCGCACCCAC CCCCAGACCC CCCGATCCTG
    GAAGGAGCTG TGGGAGGAAA CGAGGCCAGG TTGCCTACCT TCGGTTCCCA TATCCTGACT
    CCAGCTGAGA TGGAGGCGTT TAAGCAAAGG CACTCGTACC CCGAGAGATT AGTTCGCAGC
    AGGAGCTCCG ACGTCGTGTC TTCTGTCCGG CGCCCGATGA GCGACCCCAG CTGGAACCGA
    CGCCCAGGGA ATGAAGAGCG AGAACTCCCT CCAGCTGCAG CCATTGGTGC TACTTCTTTG
    GTGGCTGCGC CTCACTCTTC ATCTTCATCC CCGAGTAAGG ACTCCTCAAG AGGAGAGACT
    GAAGAACGCA AAGATAGCGA TGATGAGAAA TCAGACAGGA ACAGACCTTG GTGGAGAAAA
    CGTTTTGTTT CTGCCATGCC CAAAGCTCCT ATACCATTTA GAAAGAAAGA AAAACAAGAA
    AAAGACAAAG ATGATCTGGG GCCTGACAGA TTCTCAACAC TCACAGATGA TCCCAGCCCG
    AGACTCAGTG CACAGGCTCA GGTCGCTGAG GATATTCTGG ACAAATACAG GAATGCCATC
    AAACGCACCA GCCCCAGTGA GGGAGCTCTG GCAAACTACG AAAGTGCAGA AGTCATGGGT
    GACGGTGAAA GCGCACACGA CTCTCCTCGT GACGAAACCC TGCAGAACAT CTCCGCTGAC
    GATCTCCCAG ATTCTGCGAG CCAAGCGGCT CACCCTCAGG ATTCAGCCTT CTCTTACAGA
    GATGCAAAAA AGAAACTGAG GCTCGCTCTT TGCTCTGCGG ATTCTGTGGC TTTCCCAGTG
    CTAACCCACT CAACAAGAAA CGGTTTACCA GACCATACAG ACCCAGAAGA CAATGAAATT
    GTCTGCTTCT TAAAAGTGCA AATAGCTGAA GCAATTAACT TACAAGATAA GAACTTAATG
    GCTCAGCTTC AGGAAACAAT GCGTTGTGTG TGCCGCTTTG ATAACCGGAC CTGTAGGAAG
    CTGCTGGCGT CCATCGCCGA GGACTACAGG AAGCGGGCCC CCTACATTGC TTACCTCACT
    CGCTGTCGAC AAGGACTGCA GACCACGCAG GCTCACCTGG AAAGGCTACT GCAGAGGGTT
    CTGCGGGACA AAGAGGTGGC CAATCGATAC TTCACCACCG TCTGTGTGAG GCTGCTGCTT
    GAGAGCAAAG AGAAGAAGAT CAGGGAATTC ATTCAAGACT TTCAGAAACT CACAGCCGCT
    GATGATAAAA CCGCCCAAGT GGAAGATTTT CTGCAGTTCC TGTACGGCGC GATGGCCCAG
    GACGTCATAT GGCAGAACGC GAGTGAGGAA CAGCTTCAGG ACGCTCAGCT GGCCATTGAG
    AGAAGTGTGA TGAACCGGAT CTTCAAACTT GCCTTCTACC CAAATCAGGA CGGGGACATA
    CTCCGTGACC AGGTTCTTCA TGAACATATT CAGAGATTGT CTAAAGTAGT GACTGCAAAT
    CACAGAGCTC TGCAGATACC AGAGGTTTAT CTTAAGGAGG CACCGTGGCC CTCGGCACAG
    TCAGAAATCA GGACAATAAG TGCTTACAAG ACGCCCCGGG ACAAAGTGCA GTGCATCCTG
    AGAATGTGCT CCACGATCAT GAACCTCCTG AGCCTGGCCA ACGAGGACTC CGTCCCAGGG
    GCAGATGACT TCGTCCCTGT GCTGGTGTTT GTGTTGATCA AGGCAAATCC ACCCTGTTTG
    CTGTCCACTG TTCAGTACAT CAGTAGCTTT TATGCCAGCT GTCTGTCTGG AGAGGAGTCC
    TATTGGTGGA TGCAGTTCAC GGCGGCCGTG GAATTCATTA AGACTATCGA TGACCGAAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54406
    Clone ID Related Accession (Same CDS sequence) XM_010810355.3 , XM_010810355.2
    Accession Version XM_010810355.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4320bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810355.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGAT GATCCCAGCC CGAGACTCAG TGCACAGGCT
    CAGGTCGCTG AGGATATTCT GGACAAATAC AGGAATGCCA TCAAACGCAC CAGCCCCAGT
    GAGGGAGCTC TGGCAAACTA CGAAAGTGCA GAAGTCATGG GTGACGGTGA AAGCGCACAC
    GACTCTCCTC GTGACGAAAC CCTGCAGAAC ATCTCCGCTG ACGATCTCCC AGATTCTGCG
    AGCCAAGCGG CTCACCCTCA GGATTCAGCC TTCTCTTACA GAGATGCAAA AAAGAAACTG
    AGGCTCGCTC TTTGCTCTGC GGATTCTGTG GCTTTCCCAG TGCTAACCCA CTCAACAAGA
    AACGGTTTAC CAGACCATAC AGACCCAGAA GACAATGAAA TTGTCTGCTT CTTAAAAGTG
    CAAATAGCTG AAGCAATTAA CTTACAAGAT AAGAACTTAA TGGCTCAGCT TCAGGAAACA
    ATGCGTTGTG TGTGCCGCTT TGATAACCGG ACCTGTAGGA AGCTGCTGGC GTCCATCGCC
    GAGGACTACA GCGTCTTTCC TTTCAGGAAG CGGGCCCCCT ACATTGCTTA CCTCACTCGC
    TGTCGACAAG GACTGCAGAC CACGCAGGCT CACCTGGAAA GGCTACTGCA GAGGGTTCTG
    CGGGACAAAG AGGTGGCCAA TCGATACTTC ACCACCGTCT GTGTGAGGCT GCTGCTTGAG
    AGCAAAGAGA AGAAGATCAG GGAATTCATT CAAGACTTTC AGAAACTCAC AGCCGCTGAT
    GATAAAACCG CCCAAGTGGA AGATTTTCTG CAGTTCCTGT ACGGCGCGAT GGCCCAGGAC
    GTCATATGGC AGAACGCGAG TGAGGAACAG CTTCAGGACG CTCAGCTGGC CATTGAGAGA
    AGTGTGATGA ACCGGATCTT CAAACTTGCC TTCTACCCAA ATCAGGACGG GGACATACTC
    CGTGACCAGG TTCTTCATGA ACATATTCAG AGATTGTCTA AAGTAGTGAC TGCAAATCAC
    AGAGCTCTGC AGATACCAGA GGTTTATCTT AAGGAGGCAC CGTGGCCCTC GGCACAGTCA
    GAAATCAGGA CAATAAGTGC TTACAAGACG CCCCGGGACA AAGTGCAGTG CATCCTGAGA
    ATGTGCTCCA CGATCATGAA CCTCCTGAGC CTGGCCAACG AGGACTCCGT CCCAGGGGCA
    GATGACTTCG TCCCTGTGCT GGTGTTTGTG TTGATCAAGG CAAATCCACC CTGTTTGCTG
    TCCACTGTTC AGTACATCAG TAGCTTTTAT GCCAGCTGTC TGTCTGGAGA GGAGTCCTAT
    TGGTGGATGC AGTTCACGGC GGCCGTGGAA TTCATTAAGA CTATCGATGA CCGAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808657.1
    CDS184..4503
    Translation

    Target ORF information:

    RefSeq Version XM_010810355.3
    Organism Bos taurus(cattle)
    Definition Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810355.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGAT GATCCCAGCC CGAGACTCAG TGCACAGGCT
    CAGGTCGCTG AGGATATTCT GGACAAATAC AGGAATGCCA TCAAACGCAC CAGCCCCAGT
    GAGGGAGCTC TGGCAAACTA CGAAAGTGCA GAAGTCATGG GTGACGGTGA AAGCGCACAC
    GACTCTCCTC GTGACGAAAC CCTGCAGAAC ATCTCCGCTG ACGATCTCCC AGATTCTGCG
    AGCCAAGCGG CTCACCCTCA GGATTCAGCC TTCTCTTACA GAGATGCAAA AAAGAAACTG
    AGGCTCGCTC TTTGCTCTGC GGATTCTGTG GCTTTCCCAG TGCTAACCCA CTCAACAAGA
    AACGGTTTAC CAGACCATAC AGACCCAGAA GACAATGAAA TTGTCTGCTT CTTAAAAGTG
    CAAATAGCTG AAGCAATTAA CTTACAAGAT AAGAACTTAA TGGCTCAGCT TCAGGAAACA
    ATGCGTTGTG TGTGCCGCTT TGATAACCGG ACCTGTAGGA AGCTGCTGGC GTCCATCGCC
    GAGGACTACA GCGTCTTTCC TTTCAGGAAG CGGGCCCCCT ACATTGCTTA CCTCACTCGC
    TGTCGACAAG GACTGCAGAC CACGCAGGCT CACCTGGAAA GGCTACTGCA GAGGGTTCTG
    CGGGACAAAG AGGTGGCCAA TCGATACTTC ACCACCGTCT GTGTGAGGCT GCTGCTTGAG
    AGCAAAGAGA AGAAGATCAG GGAATTCATT CAAGACTTTC AGAAACTCAC AGCCGCTGAT
    GATAAAACCG CCCAAGTGGA AGATTTTCTG CAGTTCCTGT ACGGCGCGAT GGCCCAGGAC
    GTCATATGGC AGAACGCGAG TGAGGAACAG CTTCAGGACG CTCAGCTGGC CATTGAGAGA
    AGTGTGATGA ACCGGATCTT CAAACTTGCC TTCTACCCAA ATCAGGACGG GGACATACTC
    CGTGACCAGG TTCTTCATGA ACATATTCAG AGATTGTCTA AAGTAGTGAC TGCAAATCAC
    AGAGCTCTGC AGATACCAGA GGTTTATCTT AAGGAGGCAC CGTGGCCCTC GGCACAGTCA
    GAAATCAGGA CAATAAGTGC TTACAAGACG CCCCGGGACA AAGTGCAGTG CATCCTGAGA
    ATGTGCTCCA CGATCATGAA CCTCCTGAGC CTGGCCAACG AGGACTCCGT CCCAGGGGCA
    GATGACTTCG TCCCTGTGCT GGTGTTTGTG TTGATCAAGG CAAATCCACC CTGTTTGCTG
    TCCACTGTTC AGTACATCAG TAGCTTTTAT GCCAGCTGTC TGTCTGGAGA GGAGTCCTAT
    TGGTGGATGC AGTTCACGGC GGCCGTGGAA TTCATTAAGA CTATCGATGA CCGAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54406
    Clone ID Related Accession (Same CDS sequence) XM_010810355.3 , XM_010810355.2
    Accession Version XM_010810355.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4320bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104301.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGAT GATCCCAGCC CGAGACTCAG TGCACAGGCT
    CAGGTCGCTG AGGATATTCT GGACAAATAC AGGAATGCCA TCAAACGCAC CAGCCCCAGT
    GAGGGAGCTC TGGCAAACTA CGAAAGTGCA GAAGTCATGG GTGACGGTGA AAGCGCACAC
    GACTCTCCTC GTGACGAAAC CCTGCAGAAC ATCTCCGCTG ACGATCTCCC AGATTCTGCG
    AGCCAAGCGG CTCACCCTCA GGATTCAGCC TTCTCTTACA GAGATGCAAA AAAGAAACTG
    AGGCTCGCTC TTTGCTCTGC GGATTCTGTG GCTTTCCCAG TGCTAACCCA CTCAACAAGA
    AACGGTTTAC CAGACCATAC AGACCCAGAA GACAATGAAA TTGTCTGCTT CTTAAAAGTG
    CAAATAGCTG AAGCAATTAA CTTACAAGAT AAGAACTTAA TGGCTCAGCT TCAGGAAACA
    ATGCGTTGTG TGTGCCGCTT TGATAACCGG ACCTGTAGGA AGCTGCTGGC GTCCATCGCC
    GAGGACTACA GCGTCTTTCC TTTCAGGAAG CGGGCCCCCT ACATTGCTTA CCTCACTCGC
    TGTCGACAAG GACTGCAGAC CACGCAGGCT CACCTGGAAA GGCTACTGCA GAGGGTTCTG
    CGGGACAAAG AGGTGGCCAA TCGATACTTC ACCACCGTCT GTGTGAGGCT GCTGCTTGAG
    AGCAAAGAGA AGAAGATCAG GGAATTCATT CAAGACTTTC AGAAACTCAC AGCCGCTGAT
    GATAAAACCG CCCAAGTGGA AGATTTTCTG CAGTTCCTGT ACGGCGCGAT GGCCCAGGAC
    GTCATATGGC AGAACGCGAG TGAGGAACAG CTTCAGGACG CTCAGCTGGC CATTGAGAGA
    AGTGTGATGA ACCGGATCTT CAAACTTGCC TTCTACCCAA ATCAGGACGG GGACATACTC
    CGTGACCAGG TTCTTCATGA ACATATTCAG AGATTGTCTA AAGTAGTGAC TGCAAATCAC
    AGAGCTCTGC AGATACCAGA GGTTTATCTT AAGGAGGCAC CGTGGCCCTC GGCACAGTCA
    GAAATCAGGA CAATAAGTGC TTACAAGACG CCCCGGGACA AAGTGCAGTG CATCCTGAGA
    ATGTGCTCCA CGATCATGAA CCTCCTGAGC CTGGCCAACG AGGACTCCGT CCCAGGGGCA
    GATGACTTCG TCCCTGTGCT GGTGTTTGTG TTGATCAAGG CAAATCCACC CTGTTTGCTG
    TCCACTGTTC AGTACATCAG TAGCTTTTAT GCCAGCTGTC TGTCTGGAGA GGAGTCCTAT
    TGGTGGATGC AGTTCACGGC GGCCGTGGAA TTCATTAAGA CTATCGATGA CCGAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808657.1
    CDS174..4493
    Translation

    Target ORF information:

    RefSeq Version XM_010810355.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810355.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGAT GATCCCAGCC CGAGACTCAG TGCACAGGCT
    CAGGTCGCTG AGGATATTCT GGACAAATAC AGGAATGCCA TCAAACGCAC CAGCCCCAGT
    GAGGGAGCTC TGGCAAACTA CGAAAGTGCA GAAGTCATGG GTGACGGTGA AAGCGCACAC
    GACTCTCCTC GTGACGAAAC CCTGCAGAAC ATCTCCGCTG ACGATCTCCC AGATTCTGCG
    AGCCAAGCGG CTCACCCTCA GGATTCAGCC TTCTCTTACA GAGATGCAAA AAAGAAACTG
    AGGCTCGCTC TTTGCTCTGC GGATTCTGTG GCTTTCCCAG TGCTAACCCA CTCAACAAGA
    AACGGTTTAC CAGACCATAC AGACCCAGAA GACAATGAAA TTGTCTGCTT CTTAAAAGTG
    CAAATAGCTG AAGCAATTAA CTTACAAGAT AAGAACTTAA TGGCTCAGCT TCAGGAAACA
    ATGCGTTGTG TGTGCCGCTT TGATAACCGG ACCTGTAGGA AGCTGCTGGC GTCCATCGCC
    GAGGACTACA GCGTCTTTCC TTTCAGGAAG CGGGCCCCCT ACATTGCTTA CCTCACTCGC
    TGTCGACAAG GACTGCAGAC CACGCAGGCT CACCTGGAAA GGCTACTGCA GAGGGTTCTG
    CGGGACAAAG AGGTGGCCAA TCGATACTTC ACCACCGTCT GTGTGAGGCT GCTGCTTGAG
    AGCAAAGAGA AGAAGATCAG GGAATTCATT CAAGACTTTC AGAAACTCAC AGCCGCTGAT
    GATAAAACCG CCCAAGTGGA AGATTTTCTG CAGTTCCTGT ACGGCGCGAT GGCCCAGGAC
    GTCATATGGC AGAACGCGAG TGAGGAACAG CTTCAGGACG CTCAGCTGGC CATTGAGAGA
    AGTGTGATGA ACCGGATCTT CAAACTTGCC TTCTACCCAA ATCAGGACGG GGACATACTC
    CGTGACCAGG TTCTTCATGA ACATATTCAG AGATTGTCTA AAGTAGTGAC TGCAAATCAC
    AGAGCTCTGC AGATACCAGA GGTTTATCTT AAGGAGGCAC CGTGGCCCTC GGCACAGTCA
    GAAATCAGGA CAATAAGTGC TTACAAGACG CCCCGGGACA AAGTGCAGTG CATCCTGAGA
    ATGTGCTCCA CGATCATGAA CCTCCTGAGC CTGGCCAACG AGGACTCCGT CCCAGGGGCA
    GATGACTTCG TCCCTGTGCT GGTGTTTGTG TTGATCAAGG CAAATCCACC CTGTTTGCTG
    TCCACTGTTC AGTACATCAG TAGCTTTTAT GCCAGCTGTC TGTCTGGAGA GGAGTCCTAT
    TGGTGGATGC AGTTCACGGC GGCCGTGGAA TTCATTAAGA CTATCGATGA CCGAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54408
    Clone ID Related Accession (Same CDS sequence) XM_015473587.1 , XM_024999024.1
    Accession Version XM_015473587.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104301.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGAT GATCCCAGCC CGAGACTCAG TGCACAGGCT
    CAGGTCGCTG AGGATATTCT GGACAAATAC AGGAATGCCA TCAAACGCAC CAGCCCCAGT
    GAGGGAGCTC TGGCAAACTA CGAAAGTGCA GAAGTCATGG GTGACGGTGA AAGCGCACAC
    GACTCTCCTC GTGACGAAAC CCTGCAGAAC ATCTCCGCTG ACGATCTCCC AGATTCTGCG
    AGCCAAGCGG CTCACCCTCA GGATTCAGCC TTCTCTTACA GAGATGCAAA AAAGAAACTG
    AGGCTCGCTC TTTGCTCTGC GGATTCTGTG GCTTTCCCAG TGCTAACCCA CTCAACAAGA
    AACGGTTTAC CAGACCATAC AGACCCAGAA GACAATGAAA TTGTCTGCTT CTTAAAAGTG
    CAAATAGCTG AAGCAATTAA CTTACAAGAT AAGAACTTAA TGGCTCAGCT TCAGGAAACA
    ATGCGTTGTG TGTGCCGCTT TGATAACCGG ACCTGTAGGA AGCTGCTGGC GTCCATCGCC
    GAGGACTACA GGAAGCGGGC CCCCTACATT GCTTACCTCA CTCGCTGTCG ACAAGGACTG
    CAGACCACGC AGGCTCACCT GGAAAGGCTA CTGCAGAGGG TTCTGCGGGA CAAAGAGGTG
    GCCAATCGAT ACTTCACCAC CGTCTGTGTG AGGCTGCTGC TTGAGAGCAA AGAGAAGAAG
    ATCAGGGAAT TCATTCAAGA CTTTCAGAAA CTCACAGCCG CTGATGATAA AACCGCCCAA
    GTGGAAGATT TTCTGCAGTT CCTGTACGGC GCGATGGCCC AGGACGTCAT ATGGCAGAAC
    GCGAGTGAGG AACAGCTTCA GGACGCTCAG CTGGCCATTG AGAGAAGTGT GATGAACCGG
    ATCTTCAAAC TTGCCTTCTA CCCAAATCAG GACGGGGACA TACTCCGTGA CCAGGTTCTT
    CATGAACATA TTCAGAGATT GTCTAAAGTA GTGACTGCAA ATCACAGAGC TCTGCAGATA
    CCAGAGGTTT ATCTTAAGGA GGCACCGTGG CCCTCGGCAC AGTCAGAAAT CAGGACAATA
    AGTGCTTACA AGACGCCCCG GGACAAAGTG CAGTGCATCC TGAGAATGTG CTCCACGATC
    ATGAACCTCC TGAGCCTGGC CAACGAGGAC TCCGTCCCAG GGGCAGATGA CTTCGTCCCT
    GTGCTGGTGT TTGTGTTGAT CAAGGCAAAT CCACCCTGTT TGCTGTCCAC TGTTCAGTAC
    ATCAGTAGCT TTTATGCCAG CTGTCTGTCT GGAGAGGAGT CCTATTGGTG GATGCAGTTC
    ACGGCGGCCG TGGAATTCAT TAAGACTATC GATGACCGAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329073.1
    CDS1..4305
    Translation

    Target ORF information:

    RefSeq Version XM_015473587.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473587.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGAT GATCCCAGCC CGAGACTCAG TGCACAGGCT
    CAGGTCGCTG AGGATATTCT GGACAAATAC AGGAATGCCA TCAAACGCAC CAGCCCCAGT
    GAGGGAGCTC TGGCAAACTA CGAAAGTGCA GAAGTCATGG GTGACGGTGA AAGCGCACAC
    GACTCTCCTC GTGACGAAAC CCTGCAGAAC ATCTCCGCTG ACGATCTCCC AGATTCTGCG
    AGCCAAGCGG CTCACCCTCA GGATTCAGCC TTCTCTTACA GAGATGCAAA AAAGAAACTG
    AGGCTCGCTC TTTGCTCTGC GGATTCTGTG GCTTTCCCAG TGCTAACCCA CTCAACAAGA
    AACGGTTTAC CAGACCATAC AGACCCAGAA GACAATGAAA TTGTCTGCTT CTTAAAAGTG
    CAAATAGCTG AAGCAATTAA CTTACAAGAT AAGAACTTAA TGGCTCAGCT TCAGGAAACA
    ATGCGTTGTG TGTGCCGCTT TGATAACCGG ACCTGTAGGA AGCTGCTGGC GTCCATCGCC
    GAGGACTACA GGAAGCGGGC CCCCTACATT GCTTACCTCA CTCGCTGTCG ACAAGGACTG
    CAGACCACGC AGGCTCACCT GGAAAGGCTA CTGCAGAGGG TTCTGCGGGA CAAAGAGGTG
    GCCAATCGAT ACTTCACCAC CGTCTGTGTG AGGCTGCTGC TTGAGAGCAA AGAGAAGAAG
    ATCAGGGAAT TCATTCAAGA CTTTCAGAAA CTCACAGCCG CTGATGATAA AACCGCCCAA
    GTGGAAGATT TTCTGCAGTT CCTGTACGGC GCGATGGCCC AGGACGTCAT ATGGCAGAAC
    GCGAGTGAGG AACAGCTTCA GGACGCTCAG CTGGCCATTG AGAGAAGTGT GATGAACCGG
    ATCTTCAAAC TTGCCTTCTA CCCAAATCAG GACGGGGACA TACTCCGTGA CCAGGTTCTT
    CATGAACATA TTCAGAGATT GTCTAAAGTA GTGACTGCAA ATCACAGAGC TCTGCAGATA
    CCAGAGGTTT ATCTTAAGGA GGCACCGTGG CCCTCGGCAC AGTCAGAAAT CAGGACAATA
    AGTGCTTACA AGACGCCCCG GGACAAAGTG CAGTGCATCC TGAGAATGTG CTCCACGATC
    ATGAACCTCC TGAGCCTGGC CAACGAGGAC TCCGTCCCAG GGGCAGATGA CTTCGTCCCT
    GTGCTGGTGT TTGTGTTGAT CAAGGCAAAT CCACCCTGTT TGCTGTCCAC TGTTCAGTAC
    ATCAGTAGCT TTTATGCCAG CTGTCTGTCT GGAGAGGAGT CCTATTGGTG GATGCAGTTC
    ACGGCGGCCG TGGAATTCAT TAAGACTATC GATGACCGAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54408
    Clone ID Related Accession (Same CDS sequence) XM_015473587.1 , XM_024999024.1
    Accession Version XM_024999024.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GTPase-activating protein and VPS9 domain-containing protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGAT GATCCCAGCC CGAGACTCAG TGCACAGGCT
    CAGGTCGCTG AGGATATTCT GGACAAATAC AGGAATGCCA TCAAACGCAC CAGCCCCAGT
    GAGGGAGCTC TGGCAAACTA CGAAAGTGCA GAAGTCATGG GTGACGGTGA AAGCGCACAC
    GACTCTCCTC GTGACGAAAC CCTGCAGAAC ATCTCCGCTG ACGATCTCCC AGATTCTGCG
    AGCCAAGCGG CTCACCCTCA GGATTCAGCC TTCTCTTACA GAGATGCAAA AAAGAAACTG
    AGGCTCGCTC TTTGCTCTGC GGATTCTGTG GCTTTCCCAG TGCTAACCCA CTCAACAAGA
    AACGGTTTAC CAGACCATAC AGACCCAGAA GACAATGAAA TTGTCTGCTT CTTAAAAGTG
    CAAATAGCTG AAGCAATTAA CTTACAAGAT AAGAACTTAA TGGCTCAGCT TCAGGAAACA
    ATGCGTTGTG TGTGCCGCTT TGATAACCGG ACCTGTAGGA AGCTGCTGGC GTCCATCGCC
    GAGGACTACA GGAAGCGGGC CCCCTACATT GCTTACCTCA CTCGCTGTCG ACAAGGACTG
    CAGACCACGC AGGCTCACCT GGAAAGGCTA CTGCAGAGGG TTCTGCGGGA CAAAGAGGTG
    GCCAATCGAT ACTTCACCAC CGTCTGTGTG AGGCTGCTGC TTGAGAGCAA AGAGAAGAAG
    ATCAGGGAAT TCATTCAAGA CTTTCAGAAA CTCACAGCCG CTGATGATAA AACCGCCCAA
    GTGGAAGATT TTCTGCAGTT CCTGTACGGC GCGATGGCCC AGGACGTCAT ATGGCAGAAC
    GCGAGTGAGG AACAGCTTCA GGACGCTCAG CTGGCCATTG AGAGAAGTGT GATGAACCGG
    ATCTTCAAAC TTGCCTTCTA CCCAAATCAG GACGGGGACA TACTCCGTGA CCAGGTTCTT
    CATGAACATA TTCAGAGATT GTCTAAAGTA GTGACTGCAA ATCACAGAGC TCTGCAGATA
    CCAGAGGTTT ATCTTAAGGA GGCACCGTGG CCCTCGGCAC AGTCAGAAAT CAGGACAATA
    AGTGCTTACA AGACGCCCCG GGACAAAGTG CAGTGCATCC TGAGAATGTG CTCCACGATC
    ATGAACCTCC TGAGCCTGGC CAACGAGGAC TCCGTCCCAG GGGCAGATGA CTTCGTCCCT
    GTGCTGGTGT TTGTGTTGAT CAAGGCAAAT CCACCCTGTT TGCTGTCCAC TGTTCAGTAC
    ATCAGTAGCT TTTATGCCAG CTGTCTGTCT GGAGAGGAGT CCTATTGGTG GATGCAGTTC
    ACGGCGGCCG TGGAATTCAT TAAGACTATC GATGACCGAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854792.1
    CDS195..4499
    Translation

    Target ORF information:

    RefSeq Version XM_024999024.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999024.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGAAGC TAGATATTCA TACTCTGGCC CATCACCTCA AGCAGGAACG CTTATACGTG 
    AACTCTGAGA AACAGCTCAT TCAGAGGCTT AATGCAGATG TACTTAAGAC AGCCGAAAAG
    TTGTATCGTA CAGCATGGAT TGCTAAGCAG CAGAGAATTA ATTTGGATCG GCTTATCATC
    ACCAGTGCTG AAGCTTCCCC TGCTGAATGT TGCCAACATG CTAAGATTTT GGAAGACACA
    CAGTTTGTTG ATGGGTATAA GCAACTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCTTG
    AGTCGACTAA GGGAAAATCC TCGTCTTATT GCCTCCTCCT TGGTTGCTGG AGAGAAACTT
    AATCAGGAGA ACACACAGAG TGTCATTTAC ACAGTTTTTA CCTCTCTCTA TGGCAACTGC
    ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTGTTGA GATACTTGAT TGAATTTGAA
    CTTAAAGAAA GTGACAACCC CAGACGACTT TTGAGGAGAG GAACCTGTGC CTTCAGCATC
    TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTCAC AGCTACTTTA
    CATGAGCCGA TCATGCAACT GCTTGTTGAA GACGAAGATC ACCTGGAGAC AGACCCAAAC
    AAGCTGATTG AAAGGTTCTC CCCAGCTCAA CAGGAAAAAC TCTTTGGAGA GAAAGGCTCA
    GACAGGTTTA GGCAGAAGGT TCAAGAGATG GTGGATTCCA ATGAGGCCAA ACTTGTGGCC
    TTGGTGAACA AATTTATTGG TTATCTCAAA CAGAACACTT ACTGCTTCCC ACATAGTCTG
    AGGTGGATTG TGTCACAGAT GTACAAAACT CTCTCCTGTG TAGACAGACT GGAAGTTGGG
    GAGGTCAGGG CCATGTGCAC TGACCTCCTG TTGGCCTGCT TCATTTGTCC TGCAGTCGTC
    AATCCAGAAC AGTATGGAAT AATCTCCGAC GCTCCCATTA ATGAGGTGGC GAGATTTAAC
    CTGATGCAGG TTGGCCGCCT TTTGCAGCAA TTAGCAATGA CTGGCTCTGA AGAGGGAGAT
    CCCCGGACAA AGAGCAGCCT TGGAAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTTGAT
    GTTGTGATTG GAGGCCGTGC AGTGGAGACC CCGCCAATGT CCTCCGTTAA TCTCCTGGAA
    GGGTTGAGCA GAACTGTGGT TTATATAACC TACAGTCAGC TTATTACTCT GGTGAATTTT
    ATGAAGAGTG TGATGTCTGG AGATCAACTG AGAGAAGATA GAATGGCTCT TGACAACTTA
    CTGGCAAATC TACCCCAGGC AAAGCCGGGA AAAAGCAGCA GTCTGGAAAT GACCCCCTAC
    AGTACTCCTC AGCTGTCTCC AGCAGCCACT CCAGCAAATA AAAAGAATCG GTTGCCTATA
    GCAACTCGGA GCAGAAGTCG CACGAACGTG CTAATGGACT TGCATATGGA CCATGAAGGG
    TCAGCTCAAG AAACCATCCA GGAGGTGCAG CCGGAAGAGG TGTTGGTCAT TTCCTTAGGA
    ACAGGTCCCC AGCTTACTCC AGGGATGATG TCAGAAAATG AGGTCCTCAA CATGCAGCTT
    TCGGATGGAG GACAAGGAGA CGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT
    AAAACCTTGC GCTTCTCCCT GTGCAGTGAT AATCTGGAAG GAATATCTGA AGGTCCTTCA
    AATCGTTCTA ATTCAGTGTC CTCACTAGAC CTGGAGGGGG AATCTGTGTC AGAGCTTGGA
    GCAGGACCTT CTGGGAGTAA TGGAGTTGAA GCTCTACAGC TGTTAGAGCA TGAGCAAGCT
    ACCACGCAGG ATAATCTTGA TGATAAGCTC CGGAAGTTTG AAATCCGTGA CATGATGGGA
    CTGACGGATG ACAGGGATAT ATCAGAAACA GTGAGTGAGA CGTGGAGTAC GGATGTCTTG
    GGAAGTGATT TTGACCCTAA CATTGACGAA GATCGCTTGC AAGAAATCGC AGGTGCAGCA
    GCAGAGAACA TGTTAGGCAG TTTACTGTGC CTGCCAGGTT CGGGGTCAGT GCTTCTTGAC
    CCCTGCACTG GTTCCACCAT ATCAGAGACA ACAAGCGAAG CTTGGAGTGT AGAGGTATTG
    CCAAGTGACT CAGAGGCCCC AGATCTAAAG CAGGAAGAGC GACTGCAGGA ACTGGAGAGC
    TGTTCTGGAC TGGGTAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC
    AGCACACCAG GCCTCAGTGT TGTGTCGGGC ATAAGTGCAA CCTCTGAGGA TATTCCCAAT
    AAGATTGAAG ATCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGTAAAGA TTCTGTCACT
    AGTCCAGACA TGGATGAAGT CACCCATGGT GCCCACCAGC TGACCTCTCC TCCGTCTCAG
    TCAGAGTCTC TGCTTGCCAT GTTTGATCCA TTGTCTTCGC ACGAAGGGGG AGCTTCTGCT
    GTGGTAAGGC CGAAGGTTCA CTATGCCCGG CCGTCGCACC CACCCCCAGA CCCCCCGATC
    CTGGAAGGAG CTGTGGGAGG AAACGAGGCC AGGTTGCCTA CCTTCGGTTC CCATATCCTG
    ACTCCAGCTG AGATGGAGGC GTTTAAGCAA AGGCACTCGT ACCCCGAGAG ATTAGTTCGC
    AGCAGGAGCT CCGACGTCGT GTCTTCTGTC CGGCGCCCGA TGAGCGACCC CAGCTGGAAC
    CGACGCCCAG GGAATGAAGA GCGAGAACTC CCTCCAGCTG CAGCCATTGG TGCTACTTCT
    TTGGTGGCTG CGCCTCACTC TTCATCTTCA TCCCCGAGTA AGGACTCCTC AAGAGGAGAG
    ACTGAAGAAC GCAAAGATAG CGATGATGAG AAATCAGACA GGAACAGACC TTGGTGGAGA
    AAACGTTTTG TTTCTGCCAT GCCCAAAGAT GATCCCAGCC CGAGACTCAG TGCACAGGCT
    CAGGTCGCTG AGGATATTCT GGACAAATAC AGGAATGCCA TCAAACGCAC CAGCCCCAGT
    GAGGGAGCTC TGGCAAACTA CGAAAGTGCA GAAGTCATGG GTGACGGTGA AAGCGCACAC
    GACTCTCCTC GTGACGAAAC CCTGCAGAAC ATCTCCGCTG ACGATCTCCC AGATTCTGCG
    AGCCAAGCGG CTCACCCTCA GGATTCAGCC TTCTCTTACA GAGATGCAAA AAAGAAACTG
    AGGCTCGCTC TTTGCTCTGC GGATTCTGTG GCTTTCCCAG TGCTAACCCA CTCAACAAGA
    AACGGTTTAC CAGACCATAC AGACCCAGAA GACAATGAAA TTGTCTGCTT CTTAAAAGTG
    CAAATAGCTG AAGCAATTAA CTTACAAGAT AAGAACTTAA TGGCTCAGCT TCAGGAAACA
    ATGCGTTGTG TGTGCCGCTT TGATAACCGG ACCTGTAGGA AGCTGCTGGC GTCCATCGCC
    GAGGACTACA GGAAGCGGGC CCCCTACATT GCTTACCTCA CTCGCTGTCG ACAAGGACTG
    CAGACCACGC AGGCTCACCT GGAAAGGCTA CTGCAGAGGG TTCTGCGGGA CAAAGAGGTG
    GCCAATCGAT ACTTCACCAC CGTCTGTGTG AGGCTGCTGC TTGAGAGCAA AGAGAAGAAG
    ATCAGGGAAT TCATTCAAGA CTTTCAGAAA CTCACAGCCG CTGATGATAA AACCGCCCAA
    GTGGAAGATT TTCTGCAGTT CCTGTACGGC GCGATGGCCC AGGACGTCAT ATGGCAGAAC
    GCGAGTGAGG AACAGCTTCA GGACGCTCAG CTGGCCATTG AGAGAAGTGT GATGAACCGG
    ATCTTCAAAC TTGCCTTCTA CCCAAATCAG GACGGGGACA TACTCCGTGA CCAGGTTCTT
    CATGAACATA TTCAGAGATT GTCTAAAGTA GTGACTGCAA ATCACAGAGC TCTGCAGATA
    CCAGAGGTTT ATCTTAAGGA GGCACCGTGG CCCTCGGCAC AGTCAGAAAT CAGGACAATA
    AGTGCTTACA AGACGCCCCG GGACAAAGTG CAGTGCATCC TGAGAATGTG CTCCACGATC
    ATGAACCTCC TGAGCCTGGC CAACGAGGAC TCCGTCCCAG GGGCAGATGA CTTCGTCCCT
    GTGCTGGTGT TTGTGTTGAT CAAGGCAAAT CCACCCTGTT TGCTGTCCAC TGTTCAGTAC
    ATCAGTAGCT TTTATGCCAG CTGTCTGTCT GGAGAGGAGT CCTATTGGTG GATGCAGTTC
    ACGGCGGCCG TGGAATTCAT TAAGACTATC GATGACCGAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.